LOCUS       DAOSEX010000001        15847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_3034_length_15847_cov_3.975241, whole genome shotgun sequence.
ACCESSION   DAOSEX010000001 DAOSEX010000000
VERSION     DAOSEX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 15847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15847
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3034_length_15847_cov_3.975241"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /gene="mnmE"
                     /locus_tag="PK612_00005"
     CDS             complement(<1..827)
                     /gene="mnmE"
                     /locus_tag="PK612_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOH94540.1"
                     /translation="MLDTIVALATPPMMGALAIIRLSGSEAFNIVTKSFTKDLTKLEK
                     STMVYGKLFDDEELVDEVIAFCFKGPNSFTGEDVVEISCHGSMLIANEIIELMIKNGA
                     RLAGRGEFTNRAYLNGKLDLVQAEAVNDMITATTKEAKKLSLLSLVGETSKIVYPIRE
                     RIADVMSLIEVNIDYPEYRDIEEASYALVISEVDYINAEIKKLIDQGRQGQIIKEGIK
                     VAIVGAPNVGKSSLLNALLKEDKAIVTDIAGTTRDVVEGEINLHGLRLLLLDTAGIR"
     gene            complement(896..1537)
                     /locus_tag="PK612_00010"
     CDS             complement(896..1537)
                     /locus_tag="PK612_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOH94541.1"
                     /translation="MRIFEAKNLDEVLALASEDIGIQTKDLVYEVLEEKKGLFKSSIK
                     ISVYETTDYIEYAENYLRLVVESLGFTPEFTTSLDGEVIKIEMSTNHNSILIGKGGKT
                     LLALNEIVQVAMTVKFKKRIRVLLDINNYKNDRYRRVTGLARKVAGDVARSKQDATLD
                     PMPSDERRAIHNALSEYRNVTTESIGAGKERQVIIRYKKPEKKEKAPKEPQDE"
     gene            complement(1537..2916)
                     /locus_tag="PK612_00015"
     CDS             complement(1537..2916)
                     /locus_tag="PK612_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOH94542.1"
                     /translation="MKKNKKRIIKLGAIVAGLFVLSSCTASFCSAKDVGYMLYAQDSG
                     LVTEVVEGTETKVHNKVLHKIILDAQSQGYATPSLEYYEKLDQKVLDYAILNFDNSEN
                     LEGAALNAAALKQNGYLKFLGTKGATVVTAEGNELWVNWTIWNKEIGAEIGYENVPDR
                     DFANFYKTQIYNKIKANRACIALYDGEYGPEDSKIPVEAKTWKGAWKKGFIEGLIVYP
                     VAALLEFLTKSFGAGGWGQIWAILVTTIIVRGLLVLMTLKQTVGAQKMQALQPELAKI
                     QQKYPNSDTNQYERQALAQAQMALYKKHRINPLGSLLVMFIQFPIFIGVWGAMTGSAS
                     LASDSVLGLNLSAQLGQSMINGWFKGGWWTAWVLFILMTAAQFVSTKLSTWLNKSKTK
                     EIGKTTANPAADKQQRQSKMMMNIMFIMIVFMSFTLPAAMGVYWFVGAIISIVQTVVV
                     HNVMKGRKQ"
     gene            complement(2918..3241)
                     /gene="rnpA"
                     /locus_tag="PK612_00020"
     CDS             complement(2918..3241)
                     /gene="rnpA"
                     /locus_tag="PK612_00020"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOH94543.1"
                     /translation="MKREHRILKNEQFQNIIKNGKLTKTPLCYGYFAPNELETLRVGV
                     AVSKKIGNAVTRNKIKRQIRAVVRPYVKTHTGDFIIVAKKEINDSSFGDLVDAIAKIF
                     DSNGD"
     gene            complement(3265..3399)
                     /gene="rpmH"
                     /locus_tag="PK612_00025"
     CDS             complement(3265..3399)
                     /gene="rpmH"
                     /locus_tag="PK612_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOH94544.1"
                     /translation="MKRTYQPSKLRRARLHGFRARMATPGGRRVLRNRRAKGRESLTV
                     "
     gene            3906..5261
                     /gene="dnaA"
                     /locus_tag="PK612_00030"
     CDS             3906..5261
                     /gene="dnaA"
                     /locus_tag="PK612_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOH94545.1"
                     /translation="MNMSISEITQIWDRTLIKLQEKIQDRRVYGAFFPDTYIHSINNG
                     IITVIANSSLAKSMLNTNYRDVIEETVGEITQSNYQISFIEKSEIPRVSEIISEKKET
                     FFKNAKVNSNLTFDNFVVGSSSNLEAYQAAVVVANSPGKLYNPLFLYSASGLGKTHLL
                     HAIGNHVKQNSPNLKVLYITADEFTDEFIRFVRDNPGGSLKDFFRTVDVLLMDDVQFL
                     ADKKSTSETFFHLFNLLVGSGKQIVLTSDRHPSELKGIEERLVTRFNSGLQISISAPD
                     LETRKDILRMKISLNNLDIEDFDEEVITFFAENFTNNVRELEGALNRLLFYVVSIAPT
                     KHVTMEVALNSVQSLINVKDAVTKLSETKIINVVADYYNLTPALIIGRNRTSQVALAR
                     HIGMYLTREILDLPFARIGKAFGGKDHSTVINAITKVEKQLKEDPALIQAINEIKERL
                     Q"
     gene            5390..5749
                     /locus_tag="PK612_00035"
     CDS             5390..5749
                     /locus_tag="PK612_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94546.1"
                     /translation="MKFKINREQFLKGLSIASRPTQAKSPIPILGNILLSLDETGLTL
                     LGSSNELAIRTHIPMRVNDLEVIRMFLPGETLISSKTLNESIRKMEGNEIDVEIIDRT
                     IAQISDGRTEYRLGAID"
     gene            5742..6521
                     /gene="dnaN"
                     /locus_tag="PK612_00040"
     CDS             5742..6521
                     /gene="dnaN"
                     /locus_tag="PK612_00040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOH94547.1"
                     /translation="MIKDYPDIDLSQDGTVFEIGAKDFIKLVDQTSFAASVREQRPIL
                     TALNLNAVNGTLTAVATDSARLANKVVTLKDNVSFVANVPARIMNEVAKLLETMVGIE
                     TIEVSASANKINFMFDDTLVVSRLVAGEYPPTRSIIPKTHASFLEANANELLKAMDRV
                     SILYAAEKDNIVKLSLSPESVVVSARSTFSAESAREEVALCKYVGEPLEISFNYTLVS
                     SAIKATGAEDVTIGFNGGHKPFSIRVATDDSLVLIVTPVRT"
     gene            6690..6893
                     /locus_tag="PK612_00045"
     CDS             6690..6893
                     /locus_tag="PK612_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOH94548.1"
                     /translation="METKPYITLQQALKKYDLVSTGGEAKFVIQNGEVKVDGILETRR
                     GRKLYGGETITYAAQKVVVKDVD"
     gene            6883..7950
                     /gene="recF"
                     /locus_tag="PK612_00050"
     CDS             6883..7950
                     /gene="recF"
                     /locus_tag="PK612_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOH94549.1"
                     /translation="MLISDLELLNYRNYPKLNVIFTSGLNVIVGDNGVGKTNVVEAIH
                     LFAFAKSFRTNDTKALIKHDTAKAIVSGTIHIPSRTDISIELTNRSKRILINGKVLPK
                     VSQLSKYVRTTTFEPEDVLFFDASPAKRRKFLDANLSSADEVYLATLVRYEKLLKERN
                     ALLKTDDNDEALDLIDKLFIELAKTIVEKRTRFIRELNKVVNEVLLKLSDEHLSVRLT
                     YESSLGSVQNIERNGLKRLAEVRAKENILESTTLGPHRDDLIATINGHGVKEHASQGQ
                     KRLIVIALALAPYFLEKDLHKKPIIILDDVLSELDKRHQERLIELLLTCTQAFITATH
                     YDNHAHAIYEVTSDRELRRIT"
     gene            8010..9950
                     /gene="gyrB"
                     /locus_tag="PK612_00055"
     CDS             8010..9950
                     /gene="gyrB"
                     /locus_tag="PK612_00055"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010677441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOH94550.1"
                     /translation="MRARESYTAEDIQVLEGLQAVRQRPGMYIGTTGLPGLHHLVWEI
                     VDNAVDEALAGFCTTIDVSINKDGSVTVADNGRGMPVGIVSQTGKTAVETIFTVLHSG
                     GKFGKGAYQVAGGLHGVGAAVVNALSERVEVNVYKDGDEYYLAFNNDGHTEGELKKVG
                     PTNKRGTRVTFKPDPLIFVESVEFDYKTISTRLRQMAYLNRGIRINVEDRRGEELKRE
                     EFYYEGGIAEYVQHINKNRTPITQDVIFVEGKGDVEINGQKSHILVEVAIQYTTEYKE
                     TIFSFANNIQTGDGGTHEQGFRLAVGRVFNNYAKEAKIHKNEDDRFTYEDVVEGMTAI
                     ISVKHPNPQFEGQTKGKLGNTDTRAVVSQVLGEKLEEYLLENPNEAKLILEKISLSSK
                     ARRAATLAREGVRRKDGIEFTTLPGKLADCSSRNPEESELFIVEGNSAGGSAKNGRNR
                     HTQAILPLRGKILNVQKAQETRIYENAEIGNMIQAIGAGIAEEFDITKARYHKIIIMT
                     DADVDGSHIRVLLLTFFNRFMRPLIQNGYVYIAQPPLYKVDYRGKQYYCYNDEQLEKL
                     KVDLDLRPGYPFQRYKGLGEMDAHQLWETTMDPANRKLLRVHIEDAIEADQVFEDLMG
                     EKVEPRKEFINENALFVKNLDV"
     gene            9961..12489
                     /gene="gyrA"
                     /locus_tag="PK612_00060"
     CDS             9961..12489
                     /gene="gyrA"
                     /locus_tag="PK612_00060"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH94551.1"
                     /translation="MVEDNIKDMLEEEEKHELEGIDTGIHPVDVVDEVRKSFLDYAMS
                     VIVSRAIPDVRDGFKPVHRRIVYGMYESGMTPDKPFKKSARIVGDVMGKYHPHGDQAI
                     YSTLVRMAQPFSLRYPLVDGHGNFGSIDGDEAAAMRYTEARMEKLATEMVRDINLNTV
                     DFGGNYDGSEKEPEVLPSRFPNLIVNGSDGIAVGMATYMVPHNLSETIDAINLVAKNP
                     DVTPREIFENGMLGPDFPTGASILGRKGILDYFETGEGSIIVRAKTHFETKGDRTWIV
                     IDEMPYKLNKATTIEAIALLVRNKEIDGITDIRDESNKEGIRVVIELRRDVVPEVVLN
                     NLLKQTRLQTSYKITNLCLVNKAPRILNMKELIVEYLNFQVEIIRRRTEHQLSQAEAR
                     DHIVEGLLKATDNIDEIVSIIKNSESGEEASQKLMERFLFSEIQTREVLGMTLRRLTG
                     LEIDKLNKEREQLLANIERYRLILSARDHMVDVVLEELEEIKAKYGDERRTEISDDLA
                     DIDNEDLIPVEDIVVMLTNQGYIKRVDPEEFRTIKRGGVGVKGMRTKEEEIIDIILHT
                     NTHTDVLFFTSKGKVYRKRGYQIPQYRREGKGLPVINLLNIEKEEAVRAIVSTDTYGE
                     DHYLLYVTRNGVTKRTTLAEFSRINVSGKIAIDLREGDELMDVKITDGTAIIGIGSSN
                     GKMASFYETDVRAMGRTAAGVRGIKLDEGAYVVGVTTSLEGALIFALTTKGYGKLTPA
                     NDYRLTKRGSKGVITIKESERTGTLAGIRAIKGDEDLFVTTASGMVVRTNFKEISTTS
                     RNTMGVRVIRLRPDDVVSSFAVVEKGIAFDEDDHEGEGTPSHEE"
     gene            12492..13493
                     /locus_tag="PK612_00065"
     CDS             12492..13493
                     /locus_tag="PK612_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94552.1"
                     /translation="MKILISFPHKKIDVFFEGAMLEYQIKKACQRDDVTLVTKFSPDI
                     DIANFINLNSQSSKVIRQSIAASIPTLLWMFFANNDTYARVIETRKDGELYIPLSRLE
                     LINMMDGIVVPAQEARVILRKLGVKIPIFVIYGAVDVRRIDELKTSKTDVFRRYFRIN
                     DDQKYAITVMNIKASEHIDQLNDLAAAVPNYNFYVFISGGTSFIDRLRLKAMDKLTSP
                     NLIVTKLVPEDVYRMGVIGASYFIDLGYDKMNVMTLFEPMYLKVPLILRKNVIFKEIV
                     DKTKAYIVNDYNGAAYLIRTEAETDKITEKAYEYTQNYNAALFTEAIYNLFYKIYTR"
     gene            13749..15026
                     /gene="serS"
                     /locus_tag="PK612_00070"
     CDS             13749..15026
                     /gene="serS"
                     /locus_tag="PK612_00070"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOH94553.1"
                     /translation="MLDINLIRNEAEKVKSLLFKKGWDFDPEPILSRDEKRRKLRVEV
                     EEAKAKLNKLSASVPVVKKAGGDIQAIFAEVKDLKAENEAKEKELKKLDEEVFNLLAE
                     LPNLPDEDLLAGGKENNKVVYTFGKMPKFTFPIKDHVELCESLGLIDYKRAAKISGSG
                     TWIYTKAGALLEWGLLNFFVSEHLKAGYDMILPPHLLNYESGFTAGQFPKFKEDVFWL
                     EGTEPRKFLLPTSETALVNFYRDEILKEDELPKKYFAYTPCYRSEAGSYRTEERGMIR
                     GYQFNKVEIFQYTKEQDSSKAFEEMVAHAAGLVEKLGLHFQISKLAAGDCSHSMARTY
                     DIEVYIPSMGIYKEVSSVSNARDYQARRGMARYRDKETNAVKYLHTLNGSGLATSRLI
                     PAIVEQFQQADGTVLVPEVLQAFVGTKVLHPDW"
     gene            15036..15132
                     /gene="ffs"
                     /locus_tag="PK612_00075"
     ncRNA           15036..15132
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK612_00075"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            15137..15592
                     /gene="tadA"
                     /locus_tag="PK612_00080"
     CDS             15137..15592
                     /gene="tadA"
                     /locus_tag="PK612_00080"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOH94554.1"
                     /translation="MNKDEKYMKLALKEAHKAAAIDEVPVGAVIVLNDKVIARGFNKR
                     EINNDPLGHAEMIAIRKASKKLNNWRLVDCELYVTVEPCAMCAGAIMWSRLKRVVYGT
                     GDPKGGAMGEAFNLFDERIVNHIPEIKGGVLAKECKEVIQSFFRNKRKI"
BASE COUNT         5228 a         2848 c         3133 g         4638 t
ORIGIN      
        1 tctctaattc ccgcggtatc gagaagaaga aggcgaagtc cgtgtaaatt aatttcacct
       61 tccacaacat cacgcgttgt accagcaata tcagtaacaa ttgctttatc ctctttaagt
      121 aatgcattta ataatgacga cttcccaaca ttaggagcac caacaatagc gactttaatt
      181 ccttctttga ttatttgacc ttgtcgtcct tgatcaatca atttcttaat ctccgcattt
      241 atataatcaa cttcactaat aacaagtgcg tagcttgctt cttcaatgtc gcgatactca
      301 ggataatcaa tgttaacttc gattaaactc atcacgtccg cgattctttc tcttatcgga
      361 taaacgattt tactcgtttc accgactagc gataatagtg aaagtttctt tgcttctttt
      421 gtcgtcgcgg taatcatatc gttaacagct tcggcctgca ctaaatcgag cttgccgttt
      481 aaatatgcgc gattagtaaa ctctccgcgc cccgcaaggc gtgcgccgtt tttaatcatt
      541 aattcaatta tctcgttagc gataagcatg ctgccgtgac aactaatctc gacaacgtct
      601 tcaccagtaa aagaattcgg ccctttaaaa caaaaagcaa ttacttcgtc aacaagttct
      661 tcgtcatcaa aaagtttgcc gtacaccatc gtgctttttt ctagtttagt taaatctttc
      721 gtaaaacttt tggtaacaat attaaaagcc tcagatcctg agagacggat gatggcaagg
      781 gcgcccatca ttggtggagt agcaagagca acaatcgtat ctaacatagt taaattttat
      841 catatcctat tatctaataa gaaaaaacca ccttagcggt ggtgtttatc ttgttttatt
      901 cgtcttgagg ttcttttggt gccttttctt ttttctctgg ctttttataa cgaatgatga
      961 cttgtctttc tttgcctgca ccaattgatt cagtggtaac attgcgatat tcacttaatg
     1021 cattatgaat cgcgcgcctt tcgtcgcttg gcattggatc aagtgttgcg tcttgcttag
     1081 aacgtgcgac gtcacccgca actttacgtg ctaagccagt aactctacgg taacggtcat
     1141 ttttataatt attaatatct aataatactc gaatgcgttt tttaaattta acggtcatcg
     1201 cgacttggac aatttcgttt aacgcgagta atgttttacc acccttacca ataagaattg
     1261 aattgtggtt tgtgctcatt tcaattttaa tgacttcgcc atctaaactc gtggtgaatt
     1321 caggagtaaa acctaatgac tccacgacta accgtaaata attttcagcg tattcaatgt
     1381 aatcagttgt ttcgtataca ctgattttga ttgaactttt gaaaagtcct ttcttttctt
     1441 caagtacttc atacactaag tcttttgttt gaataccaat atcttcactt gcgagcgcta
     1501 agacttcatc aaggtttttt gcttcaaata tccgcattat tgtttccttc ctttcatcac
     1561 attatgaaca actacagttt gaacaattga gataattgcc cctacaaacc aatacacacc
     1621 catcgctgca ggaagagtaa acgacataaa gacaatcata ataaacataa tattcatcat
     1681 catttttgat tgccgttgtt gtttgtccgc agctggatta gcggttgttt taccaatttc
     1741 ttttgttttg ctcttgttaa gccatgttga taattttgtg ctaacaaatt gtgcagcggt
     1801 cattaatata aataacaccc acgctgtcca ccagccaccc ttaaaccaac cattaatcat
     1861 tgattgacca agttgagcac ttaagttaag ccctaaaaca ctatcacttg ctaaactagc
     1921 agaaccagtc attgcgcccc atacccctat aaagattggg aactgaatga acattacaag
     1981 tagtgatcca agtgggttga ttctgtgttt cttatataac gccatttgtg cttgcgctaa
     2041 agcttgtctt tcatattgat tagtgtcaga gtttggatac ttttgttgaa tctttgcaag
     2101 ctctggttgt aaggcttgca tcttttgtgc acccaccgtt tgtttaagtg tcataaggac
     2161 aagtagtccc cgcacaataa ttgttgtaac taagatggcc caaatttgtc cccatccacc
     2221 agcgccaaat gattttgtta aaaattcaag tagtgctgca acaggataaa caattaagcc
     2281 ttcaatgaac cctttcttcc atgcaccttt ccatgtcttg gcttcgactg gaattttact
     2341 atcttctggt ccatattcgc cgtcataaag cgcaatacaa gcacgatttg ccttaatttt
     2401 gttataaatt tgtgttttat aaaaattagc aaagtcgcgg tcgggaacat tttcataacc
     2461 aatttctgca ccaatttctt tgttccaaat tgtccagtta acccacagtt cattaccttc
     2521 agcagtcact actgttgcgc ctttggtacc taaaaacttt aagtaaccat tttgttttaa
     2581 ggccgcagcg ttaagtgcag cgccttctaa gttttcacta ttatcaaaat ttagaattgc
     2641 ataatctaaa actttttgat ctaacttttc ataatactct aggctaggag tagcgtagcc
     2701 ttgactttga gcatctaaaa taatcttatg taaaactttg ttgtgtactt ttgtttcagt
     2761 accttcaacg acttctgtaa ctaaccctga atcttgagca taaagcatat agcccacgtc
     2821 tttcgcggaa cagaagctcg cggtacatga agaaagcacg aaaagtccgg cgacaattgc
     2881 gcctagtttt ataatccgtt ttttattctt tttcatgtta atctccgtta ctgtcgaata
     2941 ttttcgcaat tgcatctact aaatcaccga aggatgaatc attaatttcc tttttcgcca
     3001 caatgataaa gtcaccagta tgcgttttaa catacggtct aacaactgct cttatttgtc
     3061 tttttatttt attgcgggtg acggcgttac caattttctt gctaacagcg acacctacac
     3121 gtaaagtttc gagttcgtta ggagcaaagt aaccatagca aagcggggtt tttgtgagct
     3181 ttccattttt tataatgttt tggaattgct cgttttttaa aatccgatgc tcccgtttca
     3241 caactctcct caaaagtagt cagcttagac cgttaaagat tcacgacctt tagcacgtcg
     3301 attacgaaga actctgcgac cacctggagt cgccattcta gcgcggaacc cgtgaagacg
     3361 agcccgtctt aatttacttg gttgatacgt acgtttcata atatttctcc tcattcaatc
     3421 caacataaag ttgttcgcga ttaattttag tggtttacat agtaaaagtc aaccaatgac
     3481 tattaaacac gaattagtag atgcttcttt caacaacgaa taataacagg atatcgttaa
     3541 atctggcggt ctttttggct ttttttcatc attttttatt aattaattga aaaatatcca
     3601 cttttatgtg gaaaacacat acgattatgt ttaattaatc aaaaaaaccg attatatgaa
     3661 acaaaaacat tgtggacaat tttaaaacac tgtgtataac tcttgcgcaa gtccacatta
     3721 tcaacatgga aaaggttttc cacaatgtta tccacaccat tattttgtgg atttggtgga
     3781 taactacata taatcgcgct tttataagta acaaattgtg gacaaaaaaa tcttattccc
     3841 ttatctcttt atttttatcc caattatgtt aaattattgc taaagattgc ggaggcaaat
     3901 agtttatgaa catgtcaatt tcagaaatca cccaaatttg ggatcgtacg cttatcaagc
     3961 tccaagaaaa aattcaagat cgaagagttt atggagcctt cttccctgac acttatattc
     4021 attcaattaa caacggaatc attacagtta tcgctaattc tagcttagct aagtcaatgt
     4081 taaacacgaa ttatcgcgat gttatagaag aaactgttgg tgaaattacg caatctaatt
     4141 atcaaataag ctttattgaa aaaagcgaaa tcccgcgtgt tagtgaaatt attagcgaaa
     4201 agaaagagac attttttaaa aacgcgaaag ttaattctaa tcttactttt gataattttg
     4261 ttgtcggaag ctcaagcaac ctcgaagctt atcaagcagc ggttgttgtc gctaattcac
     4321 caggtaaatt atataacccg cttttccttt atagtgcctc gggtttagga aaaacgcacc
     4381 ttttacatgc gattggtaat catgttaagc aaaattcccc aaatcttaag gttttataca
     4441 ttaccgctga tgagtttact gatgaattta ttcgttttgt tcgtgataat ccgggcggtt
     4501 cgctcaaaga ctttttccgt actgttgatg ttttattaat ggatgatgtc caatttttag
     4561 cggataaaaa gtcgacaagt gaaacatttt tccatctttt taacttattg gtcggctctg
     4621 gtaaacaaat tgtcttaact agcgaccgtc acccaagcga acttaaaggt attgaagagc
     4681 gtttagtgac acgctttaac tctggtttac aaattagtat ctccgctcct gacttagaaa
     4741 caagaaaaga tattttacga atgaaaatat ccttaaataa tttagatatt gaagattttg
     4801 atgaagaagt tattactttc tttgccgaaa actttacaaa taatgtgcgt gaactcgaag
     4861 gtgcgctcaa tcgtttacta ttttatgtag taagcatcgc cccaacaaaa cacgttacga
     4921 tggaagtagc actcaatagt gtgcagtctt taatcaatgt taaagacgct gtcacgaaat
     4981 taagtgaaac aaaaattatt aatgttgtcg ctgactacta taaccttact ccagcgctta
     5041 ttattggtcg taatcgcaca agccaagttg cgcttgctcg ccatatcgga atgtacttaa
     5101 ctcgcgagat tttagacctt ccgttcgcgc gcatcggcaa agcgtttggg ggaaaagatc
     5161 atagtactgt gattaacgct attacaaaag tggaaaaaca gttgaaagaa gatcctgctt
     5221 taattcaagc tataaacgaa ataaaagagc gtttacaata gatttatata ataaagtatt
     5281 tgcttctgtg ctaaaattaa aaagagtcaa taagcttttt tgtggagtta tccacagtct
     5341 ccacagccct tattattatc attaaatatg aaaggaatta aatattatta tgaagtttaa
     5401 gatcaatcga gaacaatttc taaaaggttt atcgatagcc tctagaccaa ctcaagcaaa
     5461 gtctcctatt cccattttag gaaacatttt attgtcatta gatgaaactg gattaacatt
     5521 acttggttca agcaacgaac tagcgatccg cacgcatatt ccaatgcggg ttaatgatct
     5581 cgaagtcatt cggatgttct taccaggtga aacacttatt tccagtaaaa cacttaatga
     5641 atcaataaga aaaatggaag ggaacgaaat tgatgtcgag attattgacc gtaccatcgc
     5701 acaaattagt gacggtcgaa cagaatatcg actcggcgcg attgattaaa gactatccag
     5761 atattgattt aagtcaagat ggcaccgtat ttgaaattgg cgctaaagat tttattaaac
     5821 tcgttgatca aacttcattt gccgctagtg tgagagaaca aaggccaatc ttaaccgcat
     5881 taaatttaaa tgcagttaat ggtactttaa cggcagttgc aacagacagt gcaagactag
     5941 caaacaaagt tgtgacatta aaagacaatg tttcgtttgt cgctaatgtt ccagcaagaa
     6001 ttatgaacga agtcgctaag cttcttgaaa caatggttgg cattgaaaca atcgaagtta
     6061 gtgcgtccgc caataaaatt aatttcatgt ttgatgatac actcgttgtc tcgcggcttg
     6121 tcgctggtga atatccacca actcgtagca ttatcccaaa aacacacgct agtttcctag
     6181 aagctaacgc taatgaactc ttaaaagcga tggaccgtgt ttcaattctt tacgcagcgg
     6241 aaaaagataa tatcgttaaa ctgtcattaa gtccagaaag tgttgttgtc tctgcgcgtt
     6301 caacattcag cgcggaaagt gcacgcgaag aagtagcgct ttgtaagtat gtcggtgaac
     6361 cacttgaaat ttcatttaac tatacattag taagctctgc aattaaagca acgggtgctg
     6421 aagatgtcac catcgggttt aacggcggtc acaaaccatt tagcatcaga gtagcgacag
     6481 atgactcgct tgtgttaatt gttacaccag ttcggacata attaaaatag agcaacttag
     6541 agcgaaaatt aataaaaaac gggcaaatct tcacgattca agcaaaagtc gttctaagga
     6601 cacaaaaagt agtaaattta agggaatatt tatattccct tttctttttt gtggtataat
     6661 acgttcatag cgacaaggag aacctataag tggaaacaaa accatatata actttacagc
     6721 aagcactaaa gaaatatgat ctcgtttcaa caggcggaga agcgaagttt gtaattcaaa
     6781 acggcgaagt aaaagttgac ggaatccttg aaactagacg gggtcgtaag ctttatggcg
     6841 gagagacaat tacttacgca gcacaaaagg tagtagtgaa agatgttgat tagcgatctt
     6901 gaactattaa actatcgcaa ctacccaaag cttaatgtca tcttcaccag tggactaaac
     6961 gtcatcgttg gtgataacgg tgtcggtaag acaaatgttg tagaagcgat tcatctcttt
     7021 gcgttcgcta aaagctttcg aacaaacgat acaaaagcac taataaagca cgataccgca
     7081 aaagcgattg taagcggaac aattcatata ccaagtcgga cagatataag tattgagtta
     7141 acaaaccgca gcaaaaggat actaataaat gggaaagtgc tcccgaaggt gtcgcaatta
     7201 agtaagtatg ttcggacgac aaccttcgaa ccagaagatg tactcttctt cgacgcaagt
     7261 cccgctaaaa gacgaaagtt cttagatgca aacttaagta gcgctgatga agtttatctt
     7321 gcgacattag tgcgttatga aaagttgctt aaagaaagaa acgcgctcct aaaaacggat
     7381 gataacgatg aagcactcga cttaatcgat aaactattca tcgaactagc aaaaacgatt
     7441 gtagaaaaac gcacaaggtt catccgtgag ttaaacaaag tagttaatga agtactcttg
     7501 aagttaagtg atgaacacct aagcgtaagg ctaacatatg aatcttcgct tggtagtgtc
     7561 caaaacattg agagaaacgg attaaaacga ctagcagaag ttagagcaaa agaaaacatt
     7621 ctcgaaagta caacactagg accgcatcgt gacgacttaa tagcaacaat taatggtcac
     7681 ggtgtaaaag aacacgcatc acaaggccaa aaaagattaa ttgttatcgc cttagcccta
     7741 gcgccttact tcttagagaa ggacttacac aaaaaaccga ttattattct tgatgatgtc
     7801 ttaagtgaat tagataagcg tcatcaagaa cgattaatag agttattact cacatgcact
     7861 caagcattta ttaccgcaac acattatgac aatcacgctc atgcaattta tgaagttaca
     7921 agtgaccgag aactaaggag gattacctaa tgagtgaaga gaaaaaacat aaaatagtag
     7981 atgaagaact agagcaagct aaacaagaaa tgcgcgctcg tgaaagttac actgctgaag
     8041 acattcaagt attagaaggg ttgcaagctg tacgacaaag acccgggatg tacattggga
     8101 caacaggact tcctggactc caccatttag tgtgggaaat cgttgataac gcagttgacg
     8161 aagcactagc aggcttttgt accacaattg atgtctcaat taacaaagac ggttcggtaa
     8221 cagtagcgga taacggccgc ggaatgccgg ttggtattgt ctcacaaact ggaaaaacag
     8281 ctgtagaaac aatctttact gtattacact ctggcggtaa attcgggaaa ggcgcttacc
     8341 aagtagcagg tggtctccac ggtgttggtg cggcagtcgt taatgccctt agtgaaagag
     8401 ttgaagttaa tgtctataaa gacggagatg aatactactt agcgtttaat aacgatggtc
     8461 acactgaagg cgaattaaaa aaggttggtc ccacaaacaa acgcggtaca agagtaacat
     8521 ttaaacctga tccacttatc tttgtcgaat cagtggaatt tgattacaaa acaatcagca
     8581 cgcgactaag acaaatggct tatttaaatc gcgggattag aataaatgtt gaagatcgcc
     8641 gtggcgaaga gcttaaacgc gaagaatttt attacgaagg cggtattgca gaatacgtcc
     8701 aacacattaa taaaaataga acaccgatta ctcaagatgt tatttttgtt gagggcaaag
     8761 gtgatgtcga aattaacgga caaaaaagcc acatcttagt ggaagtagcg attcagtaca
     8821 cgactgaata taaagaaacg attttctcgt tcgctaataa cattcaaact ggggacggcg
     8881 gaacacatga acaaggtttc cgtcttgcgg tcggccgtgt atttaacaat tatgcgaaag
     8941 aagcaaaaat tcataaaaat gaagacgacc gttttactta tgaagacgtc gttgaaggaa
     9001 tgacggcgat tatttccgtt aaacacccaa acccacaatt tgaaggacaa acaaaaggaa
     9061 agctcggtaa cactgatact cgggctgttg tcagtcaagt gttaggggaa aaactcgaag
     9121 agtacttgct tgaaaatcca aatgaagcga aacttatttt agagaagatt tcactttcat
     9181 ctaaagcgcg ccgcgctgca acattagcgc gtgaaggtgt gcgtcgtaaa gacgggattg
     9241 aatttacaac cttgccagga aaactagcag attgtagtag tcgtaatcct gaagaaagcg
     9301 aactatttat cgttgaaggt aatagtgcgg gcggtagtgc taaaaacggt cgcaaccgtc
     9361 atacacaagc gatcttgccg ttacgcggaa aaatcttaaa tgtgcaaaaa gcgcaagaaa
     9421 caagaatcta tgaaaacgct gaaattggga acatgattca agcaattggt gcaggaattg
     9481 ccgaagaatt cgacataact aaagcacggt atcataaaat tattattatg acagacgcgg
     9541 acgttgatgg tagtcatatt cgcgtattac tattaacatt ctttaatcgt tttatgcgtc
     9601 cacttattca aaatggttat gtatacattg cccaaccacc gctttataaa gtagattatc
     9661 gcggtaaaca atattattgt tataacgacg aacaattaga aaagcttaaa gttgatttag
     9721 atctccgtcc aggttatcct ttccaacgct ataaagggtt aggggaaatg gacgcacatc
     9781 aattatggga aacaacaatg gatcccgcaa acagaaaact tttaagagta cacattgaag
     9841 acgcaatcga agctgatcaa gtgtttgaag atttaatggg agaaaaggtt gaaccacgca
     9901 aagagtttat taatgaaaac gccctctttg tgaaaaacct tgacgtataa ggaggacatc
     9961 atggtagaag acaatatcaa agatatgctc gaagaagaag agaagcatga attagaaggc
    10021 attgacaccg gtatacaccc ggttgatgtc gtagatgaag ttcgtaaatc atttttagat
    10081 tatgcaatgt cggtaattgt tagtcgcgct attccagatg ttagagacgg gttcaaacca
    10141 gtacatcgtc ggatagttta cgggatgtat gaatcaggaa tgacgccaga caaaccgttt
    10201 aagaaaagcg cacggattgt tggggatgtc atgggtaaat atcaccctca tggtgaccaa
    10261 gcaatttatt caacattagt caggatggcg caaccattta gtttacgtta tccgcttgtg
    10321 gacggacacg gtaacttcgg tagtattgat ggtgacgaag cagcagcgat gcgttatacc
    10381 gaagcacgga tggaaaaact tgcaacggaa atggtgcgtg atattaattt aaacactgtt
    10441 gattttggtg gtaactatga tggtagtgaa aaagaaccag aagtattacc atcacgcttc
    10501 ccaaacttaa ttgttaatgg tagtgacggg attgctgttg ggatggcaac atatatggtg
    10561 ccgcacaatt taagtgaaac aattgatgcg attaacctag tagcgaaaaa tcctgatgtt
    10621 acaccacgcg aaatttttga aaacggaatg cttggtccag acttcccaac tggtgcgagc
    10681 attttaggtc gcaaagggat tttagattat tttgaaactg gtgaaggcag tatcattgtt
    10741 cgcgctaaga cgcactttga aacaaaaggt gatcgtacat ggatcgtcat tgatgaaatg
    10801 ccttataaat taaataaagc aacaacaatt gaagcaattg cccttttagt gcgtaataaa
    10861 gaaattgacg gcattactga tattcgtgac gaatcaaaca aagaaggaat tcgtgtggtt
    10921 atcgaattac gtcgtgatgt cgtaccagaa gtcgtgttaa acaatttatt aaaacaaaca
    10981 cgtttacaaa cttcatataa aataacgaat ttatgtttag ttaataaagc tccgcgtatt
    11041 ttaaacatga aggaattaat tgttgaatat ttaaacttcc aagtggaaat tattcgtcgt
    11101 cgtacagaac atcaactttc gcaagcagaa gcgcgcgacc acattgtgga aggattacta
    11161 aaagccactg ataacattga tgaaattgta tcaattatta aaaatagtga atctggtgaa
    11221 gaagcgagtc aaaagcttat ggaacgcttc ctctttagcg aaatccaaac acgcgaagtt
    11281 ttaggaatga cactccgtcg cttaactggt ttagaaattg acaaattaaa taaagaacgc
    11341 gaacaactac ttgcgaacat tgaacgctat cgcttaattt taagtgcacg tgaccatatg
    11401 gttgatgttg tccttgaaga gttagaagaa ataaaagcta agtatggcga tgaacgccgt
    11461 actgaaatta gtgatgacct tgctgatatt gataacgaag acttaattcc agttgaagat
    11521 atcgttgtga tgctcacaaa ccaaggctat attaaacgcg ttgatcctga agaatttaga
    11581 acgattaaac gcggtggcgt tggtgtaaaa ggaatgcgca caaaagaaga agaaataatt
    11641 gacattatct tacacacaaa tacacacact gatgtcttat tctttacaag caaagggaaa
    11701 gtatatcgta aacgcggtta tcaaatcccg caatatcgtc gtgaagggaa aggcttacca
    11761 gtcattaatc ttttaaatat tgaaaaagaa gaagcggtac gtgctattgt gtcgactgat
    11821 acatatggtg aagaccatta cctcttatat gtaacccgca acggtgtgac aaaacgtaca
    11881 actttagcgg aattctcacg cattaatgtc agtggtaaaa tcgctattga tttacgcgaa
    11941 ggcgatgaat taatggatgt taaaattact gatggcacgg caataattgg tattggttca
    12001 agtaatggta aaatggcaag tttctatgaa acggatgtgc gggcaatggg cagaactgcg
    12061 gccggagtcc gtggtattaa acttgacgaa ggtgcttatg ttgtcggcgt tacaacaagt
    12121 ttagagggtg ctttaatctt tgcgcttaca acaaaaggtt atggtaaatt aacacctgct
    12181 aacgattacc gtttaacaaa acgaggttca aaaggtgtaa ttaccattaa agaaagtgaa
    12241 cgtactggaa cacttgcggg aattagagca attaagggag atgaagactt atttgttacg
    12301 accgctagcg gaatggttgt gcgcaccaac tttaaagaaa ttagcacaac aagccgaaat
    12361 acaatgggtg tgcgtgtcat tcgtttacgc ccagatgatg ttgttagttc tttcgcagtt
    12421 gtcgaaaaag ggattgcttt tgacgaagat gatcacgaag gtgaagggac tcctagtcac
    12481 gaagaatagt tatgaaaata ttaatctctt ttccacataa aaagattgat gtcttctttg
    12541 aaggagcaat gcttgaatat caaataaaaa aagcatgcca aagagatgat gtaacacttg
    12601 ttactaaatt tagtcctgat attgatatcg ctaattttat aaatttgaat agtcaatctt
    12661 cgaaagtgat taggcaatca attgccgcaa gtatacccac gcttttatgg atgttcttcg
    12721 ctaataacga cacttacgct cgtgtgattg aaacaagaaa agatggagag ttatacattc
    12781 ctttatcgcg tcttgaatta attaatatga tggacggtat tgttgttcca gcacaagaag
    12841 cgcgtgttat tttacgaaaa ctcggtgtaa aaattccaat ctttgtaatt tatggagcgg
    12901 ttgatgttcg acggattgat gagttaaaaa caagcaaaac agatgtattt cgtcgctatt
    12961 tccgtataaa cgatgatcaa aaatatgcca taacagtgat gaacattaaa gcaagtgaac
    13021 atattgacca acttaatgac ttagccgctg ctgtaccaaa ctataatttc tatgttttca
    13081 tcagcggtgg cacatcattt attgatagat tgcggcttaa agcgatggat aaattaactt
    13141 caccgaactt aattgtgact aagcttgtac cagaagatgt ttatcggatg ggtgttattg
    13201 gtgcaagtta cttcattgat ttaggttacg acaagatgaa tgtaatgacg ctttttgagc
    13261 cgatgtactt aaaagtacca cttattttac ggaaaaatgt aatttttaaa gaaattgttg
    13321 ataagacaaa agcttacatt gtaaatgact ataacggagc ggcttactta attagaacag
    13381 aagcagaaac agataaaatt acggaaaaag cttacgaata tacacaaaat tataacgccg
    13441 ctttatttac agaagcgatt tataacttgt tttataagat atatactcgc taataaagta
    13501 aaacttgata aaataaatgt gttcagtgaa gagactagta ataattcgcg gtttaacagt
    13561 gactcgctga tagtgggaag gcgagaacta aaggttatga attccacctc ggagaacaaa
    13621 gcttaatcgc ttcgtttgtc gcgttaagac aataatgaga atacaacttt gtatcaaact
    13681 taggtggcac cacgttaaag acgtcctaat tagggcgttt tattttgttt aagaaaggag
    13741 tgcgtattat gttagatatt aatttaatta gaaatgaagc agaaaaagtt aaaagtttac
    13801 tttttaaaaa agggtgggat tttgatcctg aaccaatttt aagtcgtgat gaaaaacggc
    13861 ggaaacttcg cgttgaagtt gaagaagcta aagcaaaact caacaaatta agcgcaagtg
    13921 taccggttgt taaaaaggct ggaggcgaca ttcaggccat ttttgccgag gtaaaagacc
    13981 tgaaagcgga aaacgaagct aaggaaaaag aattaaagaa acttgatgaa gaagttttta
    14041 atttactagc agaattacca aatttaccag acgaagactt acttgcgggt ggaaaagaaa
    14101 ataataaagt tgtgtatact tttggtaaaa tgccaaagtt tacttttccg attaaagatc
    14161 atgttgaact ttgtgaaagt ttaggactta tcgattataa aagagctgca aaaatttcag
    14221 gaagcggcac gtggatttac actaaagcag gagcactact tgaatgggga cttttaaact
    14281 tttttgttag tgaacactta aaagcagggt acgacatgat tttacctccg cacttattaa
    14341 attatgaaag tggctttacc gctggacaat ttcctaaatt taaagaagat gtgttttggt
    14401 tagaaggaac agaaccgcgt aaattcttac taccaacttc ggaaacagca ctcgttaatt
    14461 tttatcgtga cgaaatatta aaagaagatg aattaccaaa gaaatacttt gcctacactc
    14521 cgtgttatcg tagcgaagca ggtagttatc gcactgaaga acgcgggatg attcgtggtt
    14581 atcaatttaa taaagttgaa atattccaat acactaaaga gcaagattca tcaaaagctt
    14641 tcgaagaaat ggtcgctcac gcagcaggtc ttgtcgagaa gttaggctta cacttccaaa
    14701 ttagtaaact tgcggcaggt gactgttcgc attcaatggc aagaacttat gatattgaag
    14761 tatatattcc gtcaatgggt atttataaag aagttagcag tgtaagtaac gctagagatt
    14821 atcaagctag gcgcggtatg gcgcgttatc gcgataaaga aacaaatgcg gtgaaatatt
    14881 tacatacact taacggttct ggactagcaa catcaagatt aataccagca attgttgaac
    14941 aattccaaca agcagacggc actgttttag ttcctgaagt tttacaagcg tttgtgggga
    15001 caaaagtttt acatcctgac tggtagttgc tataatgaag gtgccatcgc gatggttatt
    15061 cttcgaacca ggtcaggacg ggaacgtagc agccttaaga ttacttaatc atgtgcggtg
    15121 gtttttaatt taaactatga ataaagacga aaaatatatg aagctagcgc ttaaagaagc
    15181 gcacaaagca gcggcaattg atgaagtacc tgttggtgca gttatcgtgc ttaacgataa
    15241 agttattgcc cgaggtttta ataaacgcga aattaataac gacccgcttg gtcacgcgga
    15301 aatgattgcg attcgcaaag cgagtaaaaa actcaacaat tggcgacttg tcgactgtga
    15361 actctatgtc acagttgaac cgtgcgcaat gtgtgcgggc gcgataatgt ggagtcgctt
    15421 aaaacgagta gtgtacggca ctggtgatcc aaaaggcggc gccatgggtg aagcgtttaa
    15481 tcttttcgat gagcgaatag ttaaccatat accggaaata aaaggcgggg tgctcgctaa
    15541 agagtgtaag gaagtgattc aatcattttt ccgtaacaaa cgaaaaatat aatatgtata
    15601 taaataaatg tttacatacg ataattgaat aatcataatg tgcgctttct attatttgac
    15661 acaggagtag ttttgaaata tagtttaatt atgcaaaaaa gaatccttat tgtaatttcc
    15721 ttactaatta gtgttgttct taacggctgt acaaaaccac aaaaagaaca aagtgaacta
    15781 gaaaaattcc taattaaata taaagaagtg tatccagacg gatataattt tcaaacagga
    15841 ttttata
//
LOCUS       DAOSEX010000002        11381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_4809_length_11381_cov_2.928925, whole genome shotgun sequence.
ACCESSION   DAOSEX010000002 DAOSEX010000000
VERSION     DAOSEX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 11381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11381
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_11381_cov_2.928925"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1013
                     /locus_tag="PK612_00085"
     CDS             171..1013
                     /locus_tag="PK612_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94555.1"
                     /translation="MNKHGSKHITAPERGVILDMLKRDCKLKDIALAIAKDPRAVSRE
                     IRNRRLKHETEDNFINRSSKYEELCKRLTRFPFVCDGCNKRRGCRYKVKFIYEANSAH
                     NLYKRLLRDSRQGINLTEAEFRSLDKKIFAGVSKGQSLNHIVMHAEEFEVSKRTAYRY
                     IEHGILSVSNVDLKRRVRLRRRKTPKEKKVLVDSKMRLNRLFIDYIRFKAKQPGVAVV
                     QMDAIESCKLVKPCLLTLHFIDIRFMLIFLLPEKTSEHVTAVFKYLQTILTAAEYKKL
                     NWSW"
     gene            1084..1296
                     /locus_tag="PK612_00090"
     CDS             1084..1296
                     /locus_tag="PK612_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94556.1"
                     /translation="MLPKGSNLSFLTEDDVILLMNHVNSYGRELIDTTPYKLFIEYYG
                     EEIARKLKVRAIDPDDIHLKPDLFTR"
     gene            complement(1534..2067)
                     /locus_tag="PK612_00095"
     CDS             complement(1534..2067)
                     /locus_tag="PK612_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOH94557.1"
                     /translation="MLKKFLSNKQNQAEILRFIIVGVICTLVDFGVSSLIQFVIYPVA
                     EALKIGPFTITPNIFLAALFGFIFGVITNYILSVIVVFKNVENKKTSRSAKGFIIFVL
                     LSTGGFLINYAIKELGNLIIPMDTNYIWFVFIFGVATFVVLIYNYVTRKLILFKPKKE
                     EMIKSDENTTTSSQEKE"
     gene            2210..2788
                     /gene="pduL"
                     /locus_tag="PK612_00100"
     CDS             2210..2788
                     /gene="pduL"
                     /locus_tag="PK612_00100"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HOH94558.1"
                     /translation="MKKILIETSARHIHLSEKDLKILCGEDATLEVRGPLSQPGQFST
                     TTRLTIVGAKRNIERVTVLGPTRKDTQVEVSLTDAFTLGLDVPVRESGDIKGTPGVKL
                     VGPAGEVTLEQGLIVAKRHIHMTPADAKEFNVKNGEVVGVRVKTCDRSLVFEDVVVRV
                     REDFALAMHIDTDEANAAGICGEVYGEIVKLA"
     gene            2922..4247
                     /locus_tag="PK612_00105"
     CDS             2922..4247
                     /locus_tag="PK612_00105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOH94559.1"
                     /translation="MNNYDVIIVGAGTAGTYFAYLLGKRGLKVLVIDKDKEKDLAKRL
                     DIFHFTTESFARFNLPESKAGDEEFVSKFSYIYSRSALDNYEKKNFLEVSVMHLPLFI
                     KRLRNKAIKENVEFKFTTGFKDLIYDAKGRISGIETKDEKSYNARLVVDASGIPSVVR
                     RVVKDPYMETFPIGPRDKFYVFLKYVKLKDPNIKIDMSLSWPYYKSWIAPQQNKDGAI
                     VGVGANISVDYARKCMKKFEDSIKLPAYDLQYEEWGATPYRRPPYSFVSDGFLVLGDA
                     ACLTQPFNGEGITAAWVQSTPAADVVANALKDDKYPTKEALWPINVMYQTGEGALFAG
                     QRALLVGAVNMSKEDNDYLYANGIIFKSDDEEVKGSVAGNLIKGVLKGKFSFAALRSL
                     MGASSTGNKLTKHYKNYPTSPEKYFAWKKKADRLWKKAGSMADNVKDAD"
     gene            4249..4782
                     /locus_tag="PK612_00110"
     CDS             4249..4782
                     /locus_tag="PK612_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOH94560.1"
                     /translation="MKGIILYFSKYGATKKYVDWLSKSTNFDVVPVKKAKKSHIKDYD
                     VIILGSGIYASGIKGVKFLRKHAKLLSDKKVLIFCVGASPFDEKAFQTLKDHNLKDDL
                     KHIPCFYLRGAWNMDKMTFIDRTLCKLLFKMLAKKEKDELEVWEKALLAAGNRVVDWT
                     NERYLAPITKYLANLNQ"
     gene            4982..6163
                     /gene="metK"
                     /locus_tag="PK612_00115"
     CDS             4982..6163
                     /gene="metK"
                     /locus_tag="PK612_00115"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010288382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOH94561.1"
                     /translation="MANYRLFTSESVSEGHPDKVCDQISDAILDAVLAINPNARVACE
                     VFATTNFVLISGETSLTKEEEPDYENIAREVIRDIGYTNPKLGFSADAAEIVIKMNQQ
                     SSDIAQGVTQIKPEEQGAGDQGIMFGYATKESAGLMPLAIVIAHKLMRVATKMRKDGE
                     FPGARPDMKSQVTMRYYEDGSSAIDTVLMSVQHDEDVNLEEFKKFIHQKIMIPVVESF
                     GKNTDFKYLVNPTGRFVIGGPAGDTGLTGRKIIVDTYGGYAKHGGGAFSGKDPSKVDR
                     SAAYYARYIAKNLVESDVCDEVEIQLSYAIGVAEPLSVAIDTFGTGKVADEVILAAIS
                     KYFDCRPGMITQEFSLRKPKFKYRDLAALGHFGRPDINIPWEETNKAQALKSYFKKYL
                     V"
     gene            6248..6808
                     /gene="raiA"
                     /locus_tag="PK612_00120"
     CDS             6248..6808
                     /gene="raiA"
                     /locus_tag="PK612_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOH94562.1"
                     /translation="MKYQIVGKNIVVTDAIRNALEKKLSRMNKYFVIDNDIDCRAVVS
                     KIRSTAKVEVTIFTKHMIFRAEESDADLYAAFDKAIDSLEGQMRKLKTRLSRRNRVGL
                     GEAIAQAIAFDNIEDATVNEAENKVVRLKSIYLEPMSLEDAIMRMEALSHKFFLYLDA
                     EDNLISLLYIRNDGGYGIIQAENKVK"
     gene            6907..7743
                     /locus_tag="PK612_00125"
     CDS             6907..7743
                     /locus_tag="PK612_00125"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH94563.1"
                     /translation="MKKNYKLLPTDLDGTLFYPKRPRSLISRKNKKFLKKFMDEGNKV
                     VLVTGRSPAYTKNVFEVLGQEVDVIGMNGAYTIVDGQIRDEHFLDFPIEKMLYDLNAL
                     FPILGTMLISQKYPLLISTPPIGKFIQFFYNVYYFLQGSYAEDYHFSNEEFIAEIESK
                     HVYKLMIFFGMSRSGVKRAARANQYLREHYPHQFEASWTGGFIGITPVGVSKANGIKK
                     YAAEKNIPEENINVVGDSGNDISMFKAFYEHSFCMKHSHKNVKKYAKTIIRRFHHLEK
                     YI"
     gene            7757..8614
                     /locus_tag="PK612_00130"
     CDS             7757..8614
                     /locus_tag="PK612_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94564.1"
                     /translation="MNQVTQYILGIYQINMIIRDTVSYVAPRKEQSFSKEIYEHRGRS
                     LELLTAEGSPFAHFVSINKEKADKLVENINEFKKEMYSPESRVFRVVGEGIEVDDKMH
                     HRVYEMAIGIYQTLLDVLGGYIKYAKDNDQLELRIEELVAADEYFFRSLSYFALINDI
                     FKLFKEFSDAMQQHKGEPNPIAKFINEDINKMVQLIAFMNKHNRVTNLTYKKMTDLTN
                     AFIEHMGGQRQLPEGKNFPELFTELNEFALKTLQDAETNWRTIFLPVAKAHQEEMKKN
                     ERKNPEDLS"
     gene            8617..9477
                     /locus_tag="PK612_00135"
     CDS             8617..9477
                     /locus_tag="PK612_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH94565.1"
                     /translation="MRKTALIFDSTAIIDEEIVKKYDITFVSMNVAIDGVNHRALDID
                     DEEFRKQFHNLKNLKSGSPSILDFEEAINAKFDEGYKEVIVLPLASGLSATFNIAVMA
                     REGLSPERKAATYIHESNNASIGMDLLMHSLVELLDTDPTADELLAALEARAPQNRVL
                     FEIDNLRHLFRGGRLNPLKYIIGTALKIKPLVEFKDGELKVIAQNRSRRKTMDVILGK
                     IDDFVQNFKNVYVGLFTYDKEDNIFKAIHDIIKERWPHIRINFSKKICPVFTTHVGVD
                     GYSVAVVSYN"
     gene            9487..9813
                     /locus_tag="PK612_00140"
     CDS             9487..9813
                     /locus_tag="PK612_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94566.1"
                     /translation="MAKRKIDGGELALYIIYGVIALGGLTLVVLHLIGMNLANLENAL
                     RVADETFAEKMKMDFLVFGSLLVVLAGALSAITLAIYGNRAELEEEKRARRRQRMALE
                     DFSDLE"
     gene            complement(9842..10717)
                     /locus_tag="PK612_00145"
     CDS             complement(9842..10717)
                     /locus_tag="PK612_00145"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HOH94567.1"
                     /translation="MHKDKIINAFKIYLNKDEVNLEYQERFLGGMSNYTYHVKVNGKD
                     YVIRIANKEGKVFVDYATEKLHLFLLEPFNITSKTLFYDTDTGIKISEYIPGDNLTAT
                     LSAEDYAGVARALHKLHDLNIEGVDYGQIDRLARYESLVEKHLTPEYFELKAFWMNEN
                     STTYKDADIVFTHGDAQRTNIVKNGDSYTLVDFEFAGMNDRYFDIASFGNINFSDSLN
                     LLDYYLGRKALPHEVRRVKFHRLFQVLQWHVVALQKHLNGQSEKLLIDFKKYSDNYLA
                     FAAYLATKIKALPIK"
     gene            10766..10841
                     /locus_tag="PK612_00150"
     tRNA            10766..10841
                     /locus_tag="PK612_00150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10799..10801,aa:Arg,seq:cct)
     gene            10850..11203
                     /locus_tag="PK612_00155"
     CDS             10850..11203
                     /locus_tag="PK612_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOH94568.1"
                     /translation="MKRHEFTLKPIPFMRIKNKQKVIEMRLYDEKRQKLRVGDFIYFT
                     NTETKEILKVEILELTRYLNFTELYAAYTPKELGYLDGEEVAPSDMEEYYKKDDIDRF
                     GVLAIYINVLNSEID"
     gene            11242..11317
                     /locus_tag="PK612_00160"
     tRNA            11242..11317
                     /locus_tag="PK612_00160"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11275..11277,aa:Ala,seq:ggc)
BASE COUNT         3850 a         1860 c         2206 g         3465 t
ORIGIN      
        1 attttaacat attgaagaga caagaaatga aaataggctt attagcctat atatttgtta
       61 attgacaaag taaaatccgt ttttaagggg tgagcaaatt ttttgatttt tttataataa
      121 aagtgatgtt actcttgggc taccaaatac attataagga ggtgctaaac atgaataaac
      181 atggtagcaa acacatcact gcacctgaaa gaggtgttat tctcgatatg ttaaaacgag
      241 attgtaaact taaagacatc gctcttgcga tagcaaaaga tccacgcgcg gtcagtcgcg
      301 aaattagaaa tcgacgctta aaacatgaaa cagaagataa tttcatcaat cgttcatcaa
      361 agtatgaaga attgtgtaaa aggttaacac gtttcccctt tgtctgtgat ggctgtaaca
      421 aacgacgtgg ttgtcgttat aaggtgaaat ttatttatga ggcaaattcc gcacataact
      481 tatacaaacg tttactacgt gattcgcgcc aagggattaa tcttactgaa gcagaattta
      541 gaagtcttga taaaaagatt tttgctggag ttagcaaagg ccaatcgctt aatcacattg
      601 tgatgcacgc tgaagaattt gaagtttcaa agcgaacagc ttatcgctat attgaacatg
      661 gcattctctc tgtttctaat gttgatctca aacgaagagt aagactccgc agaaggaaaa
      721 caccaaaaga aaagaaagtg ctcgtcgata gtaaaatgcg cttaaatcgc ttgtttatcg
      781 actatattcg ctttaaagct aagcagcctg gtgtcgctgt ggtccaaatg gatgccattg
      841 aaagttgtaa actagttaaa ccctgtttat taacactgca tttcattgat attcgcttta
      901 tgcttatctt tcttttacct gaaaaaactt ctgagcatgt tactgcagta tttaaatatt
      961 tacaaacaat attaacagcc gcagaatata aaaaacttaa ctggtcgtgg taatgaattt
     1021 tgtgacccac aagcaagcaa ccaaaaagct caaatagaac gaaatcacgg cattatccgc
     1081 tatgtgttgc ctaaaggttc taaccttagt ttcttaaccg aagatgatgt tattttatta
     1141 atgaatcacg ttaattctta tggtcgtgaa ttaatcgata ccacacctta caaactattc
     1201 atcgaatatt atggcgaaga aattgctcgt aaattaaaag tcagggccat tgaccctgac
     1261 gatatccacc ttaaaccaga tttatttacc agatagttta gtttaaggaa tttctgcaca
     1321 agagtaacgt cggatttaag tttggcaaac ggattttgcc actccctttt tagtttgctc
     1381 cgtttctctt tgtgcaccct aattatacac accaaactca cttattagca agtccctttt
     1441 taattttcta aaatttttat ccgttttatg aatcgttttt caaaccaccc cttaaaacgg
     1501 actttacttt gtcagttaac gaaagattgt tatttactct ttttcttgac tagaagtagt
     1561 ggtgttttca tcacttttta tcatttcttc ttttttaggt ttaaataaaa taagtttacg
     1621 cgtcacatag ttataaatta agacaacgaa tgtcgcaaca ccaaagatga atacaaacca
     1681 aatataatta gtgtccatcg gaataattaa gttacctaat tctttaatgg cataatttat
     1741 taaaaaacca ccagtggata acagcacaaa aataataaaa cctttcgctg aacgggatgt
     1801 tttcttattt tcgacatttt taaaaacaac aataacggaa agaatgtagt tagtgattac
     1861 cccaaagata aaaccaaaaa gcgctgcgag aaatatattt ggtgtaattg taaagggtcc
     1921 aatcttcagt gcttcagcga cggggtaaat aacaaattga atcagtgaac taacaccaaa
     1981 atccactaaa gtacaaatta caccaacaat aatgaagcgt aatatttcag cttggttttg
     2041 tttatttgat aaaaacttct ttaacataac taagaatttc ctttctcgat aaataatata
     2101 ttatcatttt taactaaatc cgccttcaat ttcactatta atagtgagtc aatagcaaaa
     2161 taagatgatt attgttataa tttatgtgct atataaagga gaaagttaca tgaaaaaaat
     2221 acttattgaa acatctgcac gtcacattca tttaagtgaa aaagacttaa aaatattatg
     2281 tggtgaagac gctactttag aagttagagg accgcttagt caacccgggc aattttccac
     2341 gacaacacgt ttaacaatcg ttggtgcgaa acgtaatatt gaacgcgtta ctgtgttagg
     2401 accaacgcgt aaagatacgc aagttgaagt ttcactaact gatgctttta ctttaggact
     2461 agatgtccca gtgcgtgaaa gtggtgacat taaaggcacc ccaggtgtta agcttgttgg
     2521 cccagcaggt gaagttactt tagaacaagg tttaatcgtg gcaaaacgtc atatccatat
     2581 gacaccagcg gacgcaaaag agttcaatgt taaaaatggt gaagtcgtcg gcgttcgtgt
     2641 taaaacatgt gatcgtagtc ttgtgtttga agacgttgtc gtgcgggttc gtgaagactt
     2701 tgcgcttgct atgcacattg acactgacga agctaacgca gcgggtatct gtggcgaagt
     2761 ttatggcgaa attgtaaaac ttgcctagat taatctaatt attattaaaa cattaaacat
     2821 ctacttcacg agatgttttt ttgtttataa attttaaaat tatcgtaaac actaattact
     2881 tttagtaata aatatgtcac aataaaaatg gagggataca tatgaataat tatgatgtaa
     2941 ttattgtcgg ggcaggtacc gctggcactt attttgctta tttactaggg aaacgcgggc
     3001 taaaagtact tgtaatcgac aaagataaag aaaaagattt agcgaaacgc ttagacattt
     3061 tccactttac tacagaatct tttgcccgct ttaatttacc ggagtcaaaa gcaggtgatg
     3121 aagagtttgt ctcaaagttt tcttatattt attcgcgttc agcgcttgat aattatgaaa
     3181 agaaaaattt cttagaagta agcgtcatgc atttgccttt atttattaaa agactacgca
     3241 ataaggcaat caaagagaat gttgaattta agtttactac agggtttaaa gatttaatat
     3301 atgacgctaa aggacgaata agcgggatag aaacaaagga tgaaaaaagt tataacgctc
     3361 ggcttgttgt tgatgcttca ggaatcccta gtgtcgtaag aagagtagta aaagatccat
     3421 acatggaaac atttccaatt ggtccacgcg acaagttcta tgtgttctta aaatatgtaa
     3481 aactaaaaga tccgaacatt aagattgata tgtctttaag ttggccttat tacaaaagct
     3541 ggatcgcccc acaacaaaat aaagacggag caattgtcgg ggttggcgcg aatatatccg
     3601 ttgattatgc gcggaaatgt atgaagaagt ttgaagatag tattaagcta cccgcatatg
     3661 atttgcaata tgaagagtgg ggcgcaaccc catatcggcg tccgccctat agttttgtta
     3721 gtgacggttt tcttgtctta ggggatgcag catgcttaac gcaacctttt aacggtgaag
     3781 gcattaccgc tgcttgggtg caatcgacgc cagccgcaga tgtagtcgcc aacgccttaa
     3841 aagatgataa atatccaaca aaagaagcat tatggccaat taatgttatg tatcaaacgg
     3901 gagaaggtgc actctttgct ggtcaacgcg cgcttcttgt cggggccgtt aatatgagta
     3961 aagaagataa tgattactta tacgcaaacg gcattatctt taagagtgat gatgaagaag
     4021 taaaaggaag tgtagcgggt aacttaatta agggtgtctt aaaaggaaag ttctcgtttg
     4081 cggcacttcg tagtttaatg ggcgctagta gcacgggcaa caaattaaca aaacattata
     4141 aaaactatcc tacttcacca gaaaaatatt tcgcgtggaa gaaaaaagct gatcgcttgt
     4201 ggaaaaaagc aggttcaatg gcggataatg taaaggatgc tgattaagat gaaaggaata
     4261 attctctact tttctaaata cggagcgacc aaaaaatatg tcgattggct aagcaagagt
     4321 actaactttg atgttgtccc agttaaaaaa gctaaaaaga gccatatcaa agattatgat
     4381 gtaattattc ttggtagcgg tatatatgct tcaggaatta aaggtgttaa atttctgcgt
     4441 aaacacgcta aattattaag cgataaaaaa gtattaattt tttgtgttgg tgcttctccg
     4501 tttgatgaaa aagcttttca aacacttaaa gaccataatt taaaagatga ccttaaacat
     4561 ataccgtgtt tctatttgcg cggagcgtgg aacatggata agatgacatt cattgatcgc
     4621 accttatgta aattattatt taaaatgctc gctaaaaaag aaaaagacga acttgaagta
     4681 tgggaaaaag ctttgcttgc tgcgggtaat agggtagtgg actggactaa tgaacgttat
     4741 ttagcgccaa tcactaagta tcttgcaaat ctaaatcaat aagcattcgt attattttat
     4801 ttcaactata tattttgtta tacttacttt aaccttatca agaactacat tgtggcattg
     4861 atgtagtagc aactcttatg aacataagta agtgctcgct aaaggcaaat cttgtaacga
     4921 taaggaaagt gttaaattac tttccttgcg aaagtaattt taattaacgg aggataaaat
     4981 tatggctaat tatcgtttat tcactagtga atcagtaagt gagggacatc cagataaagt
     5041 ttgtgaccaa ataagtgatg caattcttga tgcagtgtta gcgattaatc ctaacgcaag
     5101 agtagcgtgt gaagtttttg ctaccactaa ttttgtctta atcagtggtg aaacctcact
     5161 aacaaaagaa gaagaaccag attatgaaaa tattgctcgt gaagtaatta gagatattgg
     5221 ctacacaaat cctaagttag gttttagcgc tgatgcggcg gagattgtga ttaaaatgaa
     5281 tcaacaaagt agtgatattg cccaaggggt aacacaaatt aaaccggaag aacaaggcgc
     5341 tggtgaccaa gggattatgt ttggttacgc tacaaaagaa agtgcagggt taatgccttt
     5401 agcaattgtt attgctcata aacttatgcg tgtcgcgact aagatgcgaa aagacggtga
     5461 gttccccggt gcaagacctg atatgaaatc gcaagtaacg atgcgctatt atgaagatgg
     5521 gagctctgca atagatacag tattaatgag tgtgcaacat gatgaagacg ttaatcttga
     5581 agagtttaaa aagtttattc atcaaaagat tatgatccca gtagtggaaa gttttggcaa
     5641 aaatactgac tttaaatatt tagttaaccc aacgggacgc tttgtcattg gtggtcctgc
     5701 tggtgatact ggtttaactg gtcgcaagat tattgtcgac acttacgggg gatacgcgaa
     5761 gcatggtggc ggtgctttta gcggtaaaga cccgagtaaa gttgaccgca gcgctgccta
     5821 ttacgcgcgt tatattgcta aaaacttagt ggaaagcgat gtgtgtgacg aagtcgaaat
     5881 tcagctaagt tacgcaatcg gagtagcgga gccactaagt gttgctattg atacatttgg
     5941 tactggtaaa gtcgcggatg aagtcatttt agcggcaatt tccaagtatt ttgactgtcg
     6001 tccaggaatg ataactcagg aatttagctt acgaaagccg aagtttaagt atcgcgacct
     6061 tgcggcgctt ggtcactttg gtcgtcccga cattaatatt ccgtgggaag aaactaataa
     6121 agcacaagca ttaaaatcat actttaaaaa atatttagtc taaattaaaa cttcctcttt
     6181 ataaattact attctcatgt taaaataaga atacataagc catctcttga tagaaaggaa
     6241 acgataaatg aaatatcaaa ttgttggcaa aaacatcgtc gtcaccgacg caataagaaa
     6301 tgcgttagaa aagaaacttt cacgcatgaa caaatacttt gtcatcgaca atgacattga
     6361 ctgtcgcgca gtagtgtcga aaattcgctc gacagcgaag gttgaagtaa caatcttcac
     6421 taagcatatg atcttccgcg cagaagaaag cgatgcagat ctctatgccg cctttgataa
     6481 ggcaattgat tcattagaag ggcaaatgcg gaaacttaaa acgaggctta gtcgacgcaa
     6541 tcgcgttggt ttaggggaag caattgctca agctattgcc tttgataata tcgaagacgc
     6601 aactgttaat gaagctgaaa acaaagtcgt acggcttaaa tcaatctatt tagaaccaat
     6661 gtcacttgaa gatgcgatta tgcgcatgga agccttgtcg cataaattct tcctttatct
     6721 tgatgctgaa gataatttaa tctcattact ttatatccgt aatgatgggg gatatggtat
     6781 tatccaagca gaaaataaag taaaatagtt aatagcgacg aagtttatca aaaaaatcgg
     6841 ctaggatatc ctagcctttt ctttatttca gtagaaataa aaaatatatt gtataattat
     6901 tacgctatga agaaaaatta caaattatta ccaactgatt tagatggaac actcttctac
     6961 ccaaagcgac cacgttcgct tatttcacga aagaataaaa agttcttaaa gaagtttatg
     7021 gatgaaggca ataaagtcgt attagtcacc ggaagaagtc cagcttatac gaaaaatgtc
     7081 ttcgaagtgc ttggccaaga agtcgatgtg attgggatga atggggccta cactattgtt
     7141 gatggccaaa ttcgtgatga acatttttta gattttccaa ttgaaaaaat gctctatgat
     7201 cttaatgcgc tctttcccat tcttggtaca atgctaatct cacaaaaata tccgctttta
     7261 atctcgactc caccgatcgg caagtttatt caattctttt acaatgttta ttacttttta
     7321 caaggtagct atgccgaaga ttatcatttt tcgaatgaag aatttattgc tgaaattgaa
     7381 tcaaaacatg tctacaaatt aatgatcttt tttggaatgt cgcggagtgg tgttaaaaga
     7441 gctgcgcgcg ctaatcaata cttgcgtgaa cattatccgc atcaatttga agcaagttgg
     7501 acgggtggat ttattggaat tacaccagta ggagtttcaa aggctaacgg gattaaaaaa
     7561 tatgcagcgg agaaaaatat tcctgaagaa aatattaatg ttgttggtga ttcggggaat
     7621 gatatttcaa tgtttaaagc attttatgag cattctttct gtatgaaaca ctcacataag
     7681 aatgttaaaa aatatgccaa aacaattatt cgacggttcc atcatttaga aaaatatatt
     7741 tagaaagaag gtatttatga atcaagttac acaatatatt ttgggtattt accaaattaa
     7801 tatgattatt cgtgatacgg taagttatgt tgcgccacgc aaggaacaat cttttagtaa
     7861 agaaatttat gaacaccgcg gacgtagtct agaactttta acagcagaag gttccccttt
     7921 tgcgcacttt gtatccatta ataaagaaaa agctgacaaa ctcgtggaaa acattaatga
     7981 atttaaaaaa gaaatgtatt caccagagtc acgagtcttt agagtagtag gagagggtat
     8041 tgaggttgat gataaaatgc atcaccgggt ttacgaaatg gcgattggga tttatcaaac
     8101 actgcttgat gttttaggcg gatacattaa atacgctaaa gataatgatc aattagaatt
     8161 acgaattgaa gaactagtgg ccgctgatga atatttcttt agaagtttat cgtactttgc
     8221 ccttattaac gatatcttta aattatttaa agagtttagt gatgcaatgc aacaacataa
     8281 gggtgaacca aatcctattg caaagtttat taacgaagac attaataaaa tggtgcaact
     8341 tattgccttt atgaataaac ataatcgtgt tactaactta acttataaga aaatgaccga
     8401 tttaacaaat gcatttattg aacatatggg tggtcagcgg caacttcctg aaggtaaaaa
     8461 cttccctgaa ttatttactg aattaaacga atttgcgctt aaaacattac aagatgctga
     8521 aactaattgg cgcacaatct ttttaccagt agcgaaagcc caccaagaag aaatgaagaa
     8581 aaatgaaaga aagaatccgg aggatttatc ataaatatga gaaaaacagc acttattttt
     8641 gattcaacag cgattattga tgaagaaatt gttaaaaaat atgacattac ttttgtatcg
     8701 atgaatgtag cgattgatgg tgtcaatcat cgcgcactcg atattgatga tgaggaattt
     8761 cgtaaacaat tccataattt aaaaaattta aaaagtggtt caccatcaat tttagatttt
     8821 gaagaagcga ttaatgctaa atttgatgaa ggatataaag aagtaatcgt gcttccatta
     8881 gcaagcggtt taagcgctac ttttaatatt gcagtaatgg cgcgtgaagg tttaagtcca
     8941 gaacgcaaag ctgctacata tatacacgaa tcaaataatg cttcaattgg gatggatcta
     9001 ttaatgcatt cacttgtgga acttttagac acagacccta ctgctgatga acttttagcg
     9061 gcgcttgaag ctcgcgctcc acaaaaccgc gtgttatttg aaattgataa tttgcgtcat
     9121 cttttccgtg gtggaaggct taatccgtta aaatacatta tcggcacagc acttaaaatc
     9181 aaaccgctcg tggagtttaa agatggtgaa ttaaaagtca ttgcgcaaaa cagaagtcgg
     9241 cgcaaaacga tggatgtaat cttaggaaaa attgatgact ttgtgcaaaa ttttaaaaat
     9301 gtttatgttg gattatttac ttatgataaa gaagacaata tatttaaagc aatccatgat
     9361 ataatcaaag aacgatggcc acatattcga attaacttta gtaaaaaaat atgtcccgtc
     9421 ttcacaaccc atgtgggagt tgatggttat tcagttgcgg ttgtaagtta taattaggag
     9481 gaagttatgg caaaaagaaa gattgatggt ggagaattag cgctttacat tatttatggc
     9541 gtgattgccc ttggtggttt aactctagtc gtattacatt taattggaat gaatcttgct
     9601 aatctagaaa acgcactgcg tgttgccgat gaaacattcg cggaaaaaat gaaaatggat
     9661 tttctcgtct ttggttcatt acttgttgtc ctagcgggcg cattaagtgc aattacttta
     9721 gcaatctatg gtaaccgcgc ggaattagaa gaagaaaaac gcgctcgtcg tcgtcaacgg
     9781 atggcgcttg aagattttag cgatttagaa taattactaa ttatttatga aaaaaagagc
     9841 actacttaat cggtagtgct tttattttag ttgctaagta agcggcaaat gcgaggtaat
     9901 tatcactata ttttttaaaa tcaattagta atttctcact ttgtccattt aaatgttttt
     9961 gtagcgctac aacatgccac tgtaatactt ggaaaagacg atgaaattta acacggcgca
    10021 cttcatgtgg taaggctttt cttcctaaat agtaatcaag aagattaaga ctatcactaa
    10081 aattaatatt accaaaacta gcaatatcaa aatagcgatc gttcattccg gcaaattcaa
    10141 aatccactag tgtataacta tcaccgtttt taacaatgtt tgtgcgttgg gcatccccat
    10201 gcgtaaaaac gatgtcagca tctttataag tcgtagagtt ttcattcatc caaaatgctt
    10261 taagttcaaa atattctggt gttaagtgtt tttctacaag tgactcataa cgcgcaagac
    10321 gatcaatttg accgtaatca acaccttcaa tatttaagtc atgcagttta tgaagggcac
    10381 gcgctacacc agcataatct tctgctgata atgtagcggt taagttatcg ccaggaatat
    10441 attcagagat tttaatcccg gtgtctgtgt cgtaaaatag tgttttactt gtaatattaa
    10501 atggctctag taaaaaaaga tgaagtttct cagttgcgta atcaacaaac accttccctt
    10561 ccttattagc aatacggata acataatctt ttccattcac ttttacgtga tatgtataat
    10621 tactcatccc accgaggaat cgttcttgat attcgagatt tacttcgtct ttattaagat
    10681 atatcttaaa agcattaata atcttgtctt tatgcatgga tatatcatac tactttttaa
    10741 ttaaatttgt tatgattaag tatgcgcgct ggtagctcag tggatagagc aatcgcctcc
    10801 taagcggtag gtcggaggtt tgaatcctcc ccagtgcgcc agccttttta tgaaaagaca
    10861 tgaatttact ttaaaaccaa ttccatttat gcgcattaaa aataaacaaa aagttataga
    10921 aatgcgcctt tatgatgaaa agcgccaaaa gcttcgcgta ggcgacttta tttattttac
    10981 taatacagag acgaaagaaa ttttaaaagt agaaatattg gaattaactc gctatctaaa
    11041 tttcactgaa ctttacgcag cttatactcc caaagaatta ggttatctcg atggtgaaga
    11101 agttgcgcct agtgatatgg aagagtatta taaaaaggac gatattgacc ggtttggcgt
    11161 tcttgctatt tatataaatg tccttaatag tgaaattgat tgacaactta tgcaaatagt
    11221 attattataa acaagcaatt aggggccgta gctcacctgg gagagcgcct ccatggcatg
    11281 gaggaggtag cgagttcgat cctcgtcggc tccaccaaaa aaatcttgat ttggtaagca
    11341 aaaaatgccg aaaaacatat aaaatcgcct taaattggcg a
//
LOCUS       DAOSEX010000003         8204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_7467_length_8204_cov_4.332188, whole genome shotgun sequence.
ACCESSION   DAOSEX010000003 DAOSEX010000000
VERSION     DAOSEX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8204
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7467_length_8204_cov_4.332188"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PK612_00165"
     CDS             complement(<1..294)
                     /locus_tag="PK612_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOH94569.1"
                     /translation="MANNKITYGLRNVHYSIATQASNGTWSFATPVALPGAQEFTSDI
                     VGGSTAVYADDIVIANLVQNAGRTLTLKLTEITEEFKTDVLGYKRLANGNLVEV"
     gene            complement(297..632)
                     /locus_tag="PK612_00170"
     CDS             complement(297..632)
                     /locus_tag="PK612_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94570.1"
                     /translation="MYLEKLRNTLLSVVPKVFYATNEYDNEDNAEPPFIVFQEISKRA
                     SVFAEDKPVFYMSTIQITLVTKKKDPALETKLKTTLLKNGYTPQNLSEFRNEDGSINK
                     IYEVRLEEI"
     gene            complement(636..1013)
                     /locus_tag="PK612_00175"
     CDS             complement(636..1013)
                     /locus_tag="PK612_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HOH94571.1"
                     /translation="MEIDKMPEALKENIQEYSENVIKQIHISLDKTADKIIDYIRSNV
                     PRSGGPNALADSFVKKAYGEGANRMIVIYSKTKSGLVHLVEFGFKHRSGKIVAARPYL
                     RPSFESLSPLMLEEIRQIIKEGG"
     gene            complement(1003..1353)
                     /locus_tag="PK612_00180"
     CDS             complement(1003..1353)
                     /locus_tag="PK612_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94572.1"
                     /translation="MISYPNSYNTSFILFRVKTAVDKLGNKSWRLVGSKEVGGVISSI
                     SSKEYYSSIQTKVLFDFKVSIQSFIYDGSKYAYFPYYGKIYEIKRIYLNGQYIELYMA
                     ETPLKWEEIINDGN"
     gene            complement(1372..1686)
                     /locus_tag="PK612_00185"
     CDS             complement(1372..1686)
                     /locus_tag="PK612_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94573.1"
                     /translation="MSENMLEIVKKALLIPVTENYADEELLIHIASCRQLLITTGVPR
                     EVAESNSDPLVKALIIIFVKTNFGFKSDGTVRELPKSFDVLLRQLCLVPLASEVHGAP
                     HE"
     gene            complement(1803..2945)
                     /locus_tag="PK612_00190"
     CDS             complement(1803..2945)
                     /locus_tag="PK612_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HOH94574.1"
                     /translation="MNLEIRKKEIAERLNEIRALAEKEDNLSVLEKFEKETDALQEER
                     NLIDKKLSMKRKFDSAAMFQIRSDAEAIENMEKRGKDLKEGRTVTVTSSNVLLPSHTN
                     AEIGAYPFRDVSTLVDKVRIVNLEGGETYKKSFVKSTDMAGLTAEGDPYTTAEPTFGY
                     ATITKVKVTAYAEITEELEKLPALPYSQEVLRGINIALRKKISQQILRGAGSTNTFKG
                     IFANNVEALADNTDIEISTIDDTTLDEIIYAYGGDEEVEGGAALILSKNDLRAFANLR
                     TTEGRKVHIVDYSASTIDGIPYVINSNCGSVVDPNTEAGTYCMAYGALTNYEVPVFSP
                     VEIAKSTDYKFKDGIISYKASVFTGGNVVGYKGFLRIKKKAATVGG"
     gene            complement(2957..3565)
                     /locus_tag="PK612_00195"
     CDS             complement(2957..3565)
                     /locus_tag="PK612_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HOH94575.1"
                     /translation="MIKNKEVRFLNIETRNEDEKESMVIEGYAIVFNQETLIGDAEYG
                     LIEIIDKNALNGANLKDVPLKYNHTDNRLILARTRNGSLTFTIDDYGLKIRADLIDTQ
                     SNRDVYKSIVAGLLDKMSFAFTVKSQSWDRSGDIPKRVITEIDRLYDVSVVDLPAYEG
                     TSIEALARSLEIADAELRALDNVKTEQLKEVIRKRLKLKTNI"
     gene            complement(3552..4778)
                     /locus_tag="PK612_00200"
     CDS             complement(3552..4778)
                     /locus_tag="PK612_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOH94576.1"
                     /translation="MGLFKRKKKTVQPINYDTKVFQSTLNIFSDFGNNINASDVVKIC
                     IDRIATHAAKLKPRYVKNLDNSSVVEKKGTLAFLLKYAPNPLMTPYDFIYRVVTLLYL
                     NNNAFIYPLYDENTYELKELWPIKPNAVEALKDNSGALFLRFSFVDGNKYTLPYESII
                     HLRRFYGVNDIFGGSGAISDHSAILKTIKINDSVLQGIDNAIRSSFQIKGLLKINAML
                     SEKDKKAQKEEFDRALKEASTSGNSAIVPVDLKSDYVPLSVDPKLVSSETLTFLQKKI
                     ITYFGVSEAIYDNKYTEDEYNAFYEGTIEGIAIALSETFSRALLTRNNLENGEQIIFY
                     SERLQYASWNTKVSAIEKLMGLGILSLNESRSLLGLEPIEGGDKRLQSLNYVDADSAN
                     KYQVGEEPKEEEDDKE"
     gene            complement(4787..6427)
                     /locus_tag="PK612_00205"
     CDS             complement(4787..6427)
                     /locus_tag="PK612_00205"
                     /inference="COORDINATES: protein motif:HMM:NF015321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HOH94577.1"
                     /translation="MSYLLDYIKEIERGNIRVGKELLSVLYALRDNMKNPDYIYDVKP
                     GQLRIKFIEKFCRHTKSPFNGQPFILELWEKAFLEASYGFKMAETGLRRFNEVLLVVA
                     RKNGKTTFIAGIDLAEFFLSSGGVDIVCASNTNEQASILFEEINNMREQSKALRNEKR
                     SKKNIFFIYSPKNKNKIKKLSAQSRNKDGYNIEVGCIDEVHEMTDSKVYDAIKQSQST
                     KKEPLIFIITTEGTTIGGFLDKKLDYARKMIKGEIKDIRILPWLYTQDSVEEVFDDPS
                     SWQKSNPSLGKIKLVSYLEDIMNKAKNDLSTRVTMLCKDFNIKQADSGSWLTYGDLNN
                     EETYNLNDLKESYAIGGVDLSSTTDLTVALLLVIKDEKKYVIPHFFMPSDVLRQRVAE
                     DNVPYDIWHKQGLITLTEGSQNDFSFVTRWFINMIQTYQIRPLWIGFDPWNSQYWIKE
                     MEESGFTMEKVRQGVYSLSEPMKQLEADLKNKVVNYNNNPIMKWCLSNTQAKVDVNGN
                     IQPSKLDSKLRRIDGTVALIIAYAVLNRYKNDYENMIS"
     gene            complement(6424..6684)
                     /locus_tag="PK612_00210"
     CDS             complement(6424..6684)
                     /locus_tag="PK612_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94578.1"
                     /translation="MEIREENGRRILYAEPGKVLQSVTDGMIIGPWLILGKNDSEIHY
                     HDIDEPIEEALPKATAPKYQAHLNDTLIEEDAPTPDSEPLEE"
     gene            complement(6699..7823)
                     /locus_tag="PK612_00215"
     CDS             complement(6699..7823)
                     /locus_tag="PK612_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOH94579.1"
                     /translation="MAYEINIHVDKKGKLVTEEVPFTISVFKESKRVKLNFTIDAEID
                     SEYHYLKFTHQKTNYLYRVHNNSFEIPKAVTAWEGRWEFSFICCDEPANSSNVITANY
                     IYASEPIVANVARGNLGNNSTTEEQNLLRELVEGTFDEFEIPNTASFISRYFLSSCPQ
                     AFTLIVPSSVITIKDRILYESGCTKIIFEEGSQLKTLEDYALYRIANLGDITFPRSID
                     SWGKYNLGFCGSGIVRFESVSNLKTLGSHAFWSIPNLTKLYLPDRLQTLAGLTAVIKN
                     CPQLNEVWIPNTITAAIPASAIQDCPLLNKITLQSNFNASANFSNVPNLTRESIVLMF
                     NALKDLSGAGAKVLTLGAGNLAKCTQEELDIALNKNWSLA"
     gene            complement(7833..>8204)
                     /locus_tag="PK612_00220"
     CDS             complement(7833..>8204)
                     /locus_tag="PK612_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94580.1"
                     /translation="QESKRPGFFIGGNMIDERVMEEYERLVDLFKDVEENKTKLVDEL
                     LMKAAFLKVQLDELEFSLKKNGAIQYSNKGNMRESVTYKTYLKTLTVYQGIIKTLSIV
                     MGKNTIDDDDEFDEFLRSANL"
BASE COUNT         2331 a         1701 c         1418 g         2754 t
ORIGIN      
        1 tacttcgaca agattgccgt tagcaagcct tttataccca agtacatccg ttttaaactc
       61 ttctgtaatt tcagtaagtt taagcgttaa agttctccct gcgttttgca ctaagtttgc
      121 aataacaatg tcgtcagcat aaaccgctgt tgaaccacca acaatatcac ttgtgaattc
      181 ttgagcgcct ggtaaggcta ctggtgtcgc aaaactccat gttccgttac ttgcttgtgt
      241 cgcaattgaa tagtggacat tacgtaaacc gtaagtaatt ttattattag ccatgtttaa
      301 atttcctcca atctaacttc atagatttta ttaattgaac catcctcatt tctaaactcg
      361 gataggtttt gtggcgtata gccattttta agaagggtcg tctttagttt agtttctagc
      421 gcaggatcct tcttcttagt aacaagcgta atttgaatag tgctcatata gaacactggt
      481 ttatcttccg cgaatactga agctctttta ctaatttctt gaaagacgat aaagggtggt
      541 tcagcattat cttcattatc gtactcatta gtagcgtaga acactttcgg gacaacgctt
      601 aagagcgtat ttcgtaattt ttctaaatac acgcattaac ctccctcttt tataatttgc
      661 cgaatttctt caagcattaa gggactaagc gattcgaaag atggtcttaa atacggtcgt
      721 gctgccacta tttttccgct tcgatgctta aaaccgaact cgactaaatg cacgagtcca
      781 cttttcgttt ttgaataaat gacaatcata cgatttgctc cttcgccata tgccttttta
      841 acaaaggaat cggcaagtgc attaggacca ccactgcgcg gaacattaga acgaatgtaa
      901 tcgataattt tatcagccgt tttatcaaga cttatatgaa tttgttttat gacattttcg
      961 gagtattctt ggatattttc ctttaatgct tctggcattt tatcaatttc catcgtttat
     1021 aatttcctcc catttaagtg gtgtttccgc catataaagt tcgatatatt gtccgtttag
     1081 atatattctt ttgatttcat agattttgcc gtagtaggga aagtaagcat acttgctccc
     1141 gtcataaata aaagactgaa tacttacctt aaaatcgaat aaaacttttg tttgaatcga
     1201 tgaataatat tccttggagg agatggagct gattactcca cccacctcct tggagccgac
     1261 caaccgccaa gacttgttgc caagtttatc gacagctgtt ttaacacgga ataggatgaa
     1321 agaagtattg taggaattag gatacgaaat cataaggaaa tccgaggatt atcactcatg
     1381 aggagcgcca tgaacctcag aggctagagg cacgaggcat aactgtctga gtaagacatc
     1441 aaagctctta ggcaactctc tcaccgtacc atcgcttttg aagccaaagt tagtcttcac
     1501 aaatatgata ataagagctt ttaccaatgg gtcactatta gattcagcga cttcacgagg
     1561 aactccagtc gttatcagca actggcggca cgaggcgata tgaattaaca attcctcatc
     1621 ggcatagttt tcggttacag ggattagcaa agcctttttt actatctcca gcatgttttc
     1681 actcatcaca atatcctccc tattttttag atattaaccc tacccgcttc aacgataggt
     1741 catgtagtac gagacgtcag acctacgtgt ttcgacctta atttaatgtt gatggggaaa
     1801 tcttatccac caacagtggc ggcttttttc ttaatgcgta agaaaccttt atagccgaca
     1861 acattaccgc cagtaaagac ggatgctttg taactgataa tcccgtcttt gaatttgtaa
     1921 tccgttgatt tagcaatttc aactggcgag aaaactggga cttcgtaatt tgttaatgcc
     1981 ccataagcca tacagtaagt accagcctca gtatttggat caacgacaga cccacagtta
     2041 ctattaatga cataaggaat gccatcaatc gttgatgcac tataatcgac aatatgaact
     2101 ttacgccctt ccgttgtccg aaggttagca aatgcccgta agtcgttctt acttaaaatt
     2161 aacgctgctc caccctcaac ttcttcatcg ccaccatagg cataaatgat ttcatcaagt
     2221 gtcgtgtcat cgattgtgct aatttcgata tcggtgttat cggcaagtgc ttcaacatta
     2281 ttagcaaaga tacctttgaa ggtattcgtg gagcctgcac cccgtaaaat ttgttgtgaa
     2341 atctttttgc ggagcgcaat attgattcct cttaacactt cttgtgagta aggtaaggct
     2401 ggaagttttt ctaattcttc cgtaatttca gcataagctg tcacttttac tttcgtaatc
     2461 gtcgcataac cgaatgtcgg ttcagcggta gtgtaaggat caccttcagc agtaagtcct
     2521 gccatatctg ttgattttac gaaggacttt ttataagtct caccaccctc taagttaacg
     2581 attcttactt tatcaacgag tgtggataca tcgcggaaag ggtatgcacc gatttcagca
     2641 ttagtgtgcg aaggaagtaa gacattactt gaagtgacag tcactgtgcg tccttctttt
     2701 aagtccttgc cacgtttctc catattttcg attgcttcag catcactacg aatttgaaac
     2761 atcgcagcac tatcgaattt acgcttcatt gataatttct tatcgattaa gtttcgttct
     2821 tcttggagcg catcagtttc tttttcgaat ttttctaata cggagaggtt gtcttctttt
     2881 tctgctaacg cccgaatttc atttaagcgt tcagcaattt cttttttacg aatttctaaa
     2941 ttcatctgaa ttcctcctaa atattcgttt ttaatttaag tcgtttgcga attacttctt
     3001 taagttgctc ggttttaaca ttatccaatg cccttagttc tgcgtccgca atttctaagg
     3061 aacgtgcgag tgcttcaata gaagttcctt catatgcagg taggtctacc acggagacat
     3121 catagagacg atcaatctcg gtaatgactc tctttggtat gtccccactt ctatcccagc
     3181 tttgactttt tacagtaaaa gcaaacgaca tcttgtctaa taatccagcg acaatcgatt
     3241 tatacacatc tcgatttgac tgggtgtcaa ttaaatccgc tctaattttt aagccataat
     3301 catcaatcgt aaaggtaagt gaaccgttac gagtacgagc taagattagg cggttatcag
     3361 tatggttata tttaagtggc acatctttta aatttgcgcc atttagtgcg tttttgtcga
     3421 tgatttcaat aagaccatat tctgcatcac caattaaagt ttcctggtta aagacaattg
     3481 cgtaaccttc aataaccatg ctttcttttt catcttcatt tcttgtttca atatttagaa
     3541 atcgcacttc tttattcttt atcatcttct tcctccttag gctcttcgcc tacttggtat
     3601 ttattagcgc tatccgcgtc aacataattt aatgactgga gacgcttgtc gccaccttca
     3661 ataggctcta agcctaataa acttcttgat tcattaagtg ataatattcc tagccccatt
     3721 aatttctcaa tcgcacttac tttggtgttc cacgatgcgt attgtagacg ctcggaataa
     3781 aagataattt gctctccatt ttctaagtta tttcttgtaa ggagagctct tgaaaaagtt
     3841 tccgatagcg caatcgcaat cccttcaatc gttccttcat agaaagcgtt atattcatct
     3901 tccgtatact tgttatcgta gattgcttca cttacaccaa aataggtgat gattttcttt
     3961 tggagaaaag ttagtgtttc gctgctaact agttttgggt caacgcttaa aggaacataa
     4021 tcacttttaa gatccacagg aacaatggca gaattaccac tagtcgaggc ttcttttaat
     4081 gccctatcaa attcttcctt ttgtgccttc ttatcttttt cagaaagcat ggcattgatt
     4141 ttaaggagcc ctttaatctg aaaactacta cgaatagcgt tatcaattcc ctgtaagact
     4201 gaatcattaa ttttgattgt tttaaggatt gcggaatggt ctgaaattgc gccgcttccg
     4261 ccaaagatat cattaacgcc gtaaaatctc cgtaaatgaa taattgattc gtaaggcaaa
     4321 gtgtatttat tcccatcaac gaaagaaaag cgaagaaata acgcaccgct attatctttg
     4381 agtgcttcaa cggcatttgg tttaattggc cataactctt ttaattcata ggtgttttca
     4441 tcatagagcg gataaataaa agcgttatta tttaagtaaa gtaatgtcac cacccgataa
     4501 ataaaatcgt aaggtgtcat taaagggtta ggcgcatact ttaaaagaaa cgctaatgtc
     4561 ccttttttct cgacaaccga agagttatca agatttttta cataacgcgg tttgagttta
     4621 gccgcgtgtg tcgcaatgcg gtcaatgcaa atcttcacca catcgctagc gtttatgttg
     4681 tttccaaaat cactaaaaat gttcaatgtc gattggaaga ctttcgtatc atagttgata
     4741 ggctgaacgg tcttcttctt tcttttaaat aatcccataa agcctcctaa cttatcatgt
     4801 tttcatagtc gtttttgtat ctgtttaaca cagcgtaagc aataattaaa gcgaccgttc
     4861 cgtcaattct tcgtaacttt gaatcaagtt tagaaggctg aatattacca ttaacatcaa
     4921 ccttggcttg tgtattactt aagcaccatt tcataatcgg gttattgtta tagttcacaa
     4981 ctttattctt taaatcggct tcaagttgtt tcattggttc agaaagcgag taaacgcctt
     5041 gtcgcacctt ttccatagtg aaacctgact cctccatctc tttaatccag tactgtgagt
     5101 tccatggatc aaaaccaatc caaagtggtc taatttgata agtttgaatc atattaataa
     5161 accacctcgt tacaaaagaa aaatcgtttt gactaccttc cgttaaagta ataagtcctt
     5221 gcttatgcca aatatcataa gggacattat cctccgctac tcgttgacga agcacatcgc
     5281 taggcataaa gaaatgcgga atgacatact ttttttcatc ttttattact agtaataacg
     5341 ctaccgttaa atcagttgta gatgataaat cgacaccgcc aatagcataa ctttctttta
     5401 aatcattaag gttataggtt tcctcattat taaggtcacc ataagtaagc cacgaaccgc
     5461 tatcagcttg cttaatatta aagtctttac aaagcatcgt aactcttgtt gagagatcat
     5521 ttttagcctt gttcatgatg tcttcaagat aggagacaag tttaatctta ccgagcgacg
     5581 gattgctttt ttgccatgag gaagggtcat cgaatacttc ttcgacacta tcttgcgtat
     5641 agagccaggg caagattcta atatccttaa tttcgccttt aatcatcttg cgagcgtaat
     5701 ctaacttttt atcaagaaag ccgccaattg tagttccctc agttgtgatg atgaaaatga
     5761 gcggttcttt cttcgttgat tgactttgct taatagcgtc ataaaccttc gaatccgtca
     5821 tttcatgcac ttcatcaata cagccaacct cgatgttata gccgtcttta ttgcgtgatt
     5881 gcgccgatag ttttttaatc ttattcttgt ttttaggcga gtagatgaag aaaatgttct
     5941 tttttgaacg cttctcattt cggagcgctt tgctctgttc acgcatatta ttaatttctt
     6001 caaaaaggat ggatgcttgc tcattagtgt ttgacgcaca gactatatct acaccacctg
     6061 aacttaagaa aaactccgct aaatcgatac ccgcaataaa ggtcgtcttc ccgtttttcc
     6121 gcgccacgac gagcaatact tcgttaaagc gacgtaaccc cgtctccgcc attttaaagc
     6181 cgtaggacgc ctctaaaaaa gccttctccc ataactccaa aatgaatggt tgcccgttaa
     6241 atggtgactt agtatgccgg caaaactttt caataaactt gatgcgtaat tggcctggtt
     6301 ttacatcata gatgtaatcg ggatttttca tgttatctct aagagcataa agtacactaa
     6361 gcaactcctt cccaacacga atatttcccc tttcaatttc tttgatgtaa tctagtaaat
     6421 aactcattcc tcaagaggct cactatcagg agtaggggcg tcttcttcaa taagggtatc
     6481 gtttaagtgc gcttgatatt ttggtgctgt cgctttaggg agtgcttctt caatcggttc
     6541 atcaatatcg tgataatgga tttcgctatc gtttttacca aggattaacc atggaccgat
     6601 aatcatgccg tctgttacgc tttgtaatac tttccctggt tcggcatata aaatgcgtct
     6661 accgttttct tctctaattt ccataaacta ataccccctt atgcaagtga ccagttttta
     6721 ttaagtgcaa tatctagttc ttcttgtgta cacttagcaa gattacctgc acctaacgtg
     6781 agcacctttg cccctgcacc acttaaatct tttaaagcgt taaacatcaa gacgattgac
     6841 tctcgtgtta gatttgggac attcgagaaa ttcgctgatg cattaaagtt gctttgcagt
     6901 gtgatcttat taagaagcgg acaatcttga attgcgctag cggggatagc agcggttata
     6961 gtatttggaa tccatacctc attaagttgc ggacagtttt taatgactgc cgttaaacca
     7021 gccaaagtct gaagacgatc tggaaggtat aatttcgtta aattcgggat actccagaaa
     7081 gcgtgacttc ccaaagtttt taaattcgaa actgattcaa accgcacgat tccgcttcca
     7141 caaaaaccta agttatattt cccccatgaa tcaattgatc tagggaacgt gatatcacca
     7201 agattcgcaa ttcgatatag cgcataatct tcaagtgttt taagttgcga cccttcttca
     7261 aatatgattt tcgtacatcc agactcatac aaaatacggt ccttaatcgt aattacactt
     7321 gagggaacaa tgagtgtgaa tgcttgcgga caacttgata aaaagtaacg actgataaat
     7381 gaagcggtgt taggaatctc aaactcatca aatgtacctt cgacaagttc acgaagtaag
     7441 ttttgttctt cagtggtcga attattacct aaattaccgc gagcgacatt tgccacaatc
     7501 ggctcacttg cataaatata attagcagta atgacattac ttgagttagc gggttcatca
     7561 caacatataa acgagaactc ccatctacct tcccaagcag ttacagcttt tggaatttcg
     7621 aaagaattat tatgcacccg atataaataa tttgtttttt gatgagtgaa ttttaaatag
     7681 tgatattcgc tatcgatttc tgcatcaatc gtgaagttta gtttcacccg cttactttct
     7741 ttaaagacgg agatagtaaa aggtacttct tctgttacga gttttccttt tttatctaca
     7801 tgaatattta tttcgtaagc cataactcct ccttaaagat ttgccgaccg taagaactca
     7861 tcaaattcat cgtcgtcatc aatcgtattt tttcccatca caatgctgag cgtttttata
     7921 attccttggt acactgtcaa agtttttaaa taagttttat aggtaactga ctcacgcata
     7981 ttacctttgt tagaatattg aatcgctcca ttctttttta aagaaaattc taattcatct
     8041 aattgaactt ttagaaaagc tgccttcatt aatagttcat ccactaattt agttttattt
     8101 tcttcaacgt ccttaaataa atctacgagg cgttcatatt cttccattac tctttcgtca
     8161 atcatatttc ctccaataaa aaatccaggt cgcttggatt cctg
//
LOCUS       DAOSEX010000004         8001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_7746_length_8001_cov_3.447269, whole genome shotgun sequence.
ACCESSION   DAOSEX010000004 DAOSEX010000000
VERSION     DAOSEX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7746_length_8001_cov_3.447269"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(613..1965)
                     /gene="rlmD"
                     /locus_tag="PK612_00225"
     CDS             complement(613..1965)
                     /gene="rlmD"
                     /locus_tag="PK612_00225"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOH94581.1"
                     /translation="MIKENDIYVVTGGDYTSEGIGLAKVDNFPIFINNLIIGEEAEIK
                     ITKVMRNFATGKIIRFTKMSSDRITPVVPETIHLAGCQFTQLDYAKEVEYKLSKVERA
                     LRVIGGIDFKIEKIHTAKNPYFYRNKTVLPFGKRKSGKVYTGLFRHNTHEIIAMEKTH
                     LDDELASTVIAEIRKLIEKYNYEVYDEKSHTGQVRKVMVRVSHYLREVMVVLITKDEQ
                     IPNLDAFCRDLVAICPNITTIIQNINSEDTNVILGKKEKVLYGPGFINEELHGLKFTL
                     SSKSFFQVNTLQAEVLYERALTLAGITKNDVVLDAYSGTGTIAILAAKKAKQVIGVEI
                     VKDAVKDAEVNAKQNNITNVKFFKDDAGAFLTREIKNYNFSVVIVDPPRKGLSDEFVN
                     TLLRALPPKIMYVSCDPATLARDLKKLTKKYNVEHIECVDMFPRTAHAETIVSLILRK
                     "
     gene            complement(1965..3545)
                     /gene="metG"
                     /locus_tag="PK612_00230"
     CDS             complement(1965..3545)
                     /gene="metG"
                     /locus_tag="PK612_00230"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOH94582.1"
                     /translation="MKKRFYITTPIYYPSAKLHIGHAYCTTLSDILARYNRMIGKEVY
                     FLTGSDEHGQKIAENAAKANKTPQKFVDDIVKTFKDLWEKLAITNDDFIRTTDPKHIA
                     TVQKIFTKLLEQGDIYLGSYEGWYCTPCESFWTTTQVGEEKLCPDCNRSVHLASEESY
                     FFKTDKYVEGLLKKYEETPNFITPESRKNEMVNTFIKPGLSELAVTRTSIEWGVPVRE
                     NERHTMYVWVDALINYISALGYLSEDDELFHKFWQSEDVEIVQILGADITRFHTIYWP
                     MILKALGLRQADRYFVHGLLMMKDGKMSKSKGNVIDPLPLIDKYGVDAVRYYLAREVV
                     FGNDGQFTPEQFVERFNVDLVNDFGNLLNRTVSMIEKYCDGVIPLYRGQVTEFDTEIE
                     DLAINVVKEYHEHFSDLKVTEAITTAFKIISASNKYIDNTKPWELNTQGKKEELDSVM
                     NHLANNLFIGAILLQPVLVNAPKELFKQLGVKEDKQDINLLNKYGVLGGEKVNKGEPL
                     FPRLKVNEEVEYIKNLMK"
     gene            complement(3606..3809)
                     /gene="rpmE"
                     /locus_tag="PK612_00235"
     CDS             complement(3606..3809)
                     /gene="rpmE"
                     /locus_tag="PK612_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOH94583.1"
                     /translation="MKQGIHPKTQKITVTCVSCGTEFEALSTKKEIRVDTCSNCHPFY
                     TGKQRFTQADGRVDKFNKKYGLK"
     gene            4631..6025
                     /locus_tag="PK612_00240"
     CDS             4631..6025
                     /locus_tag="PK612_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008979612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOH94584.1"
                     /translation="MITKKLALEVLNVALSTGGDFAEIFLEETYSGNYTVENGKTQAP
                     ATSLTYGAGIRILKGLQSVYGHTSDLSKKGLLNLATALSAGFDGQRVLSVEKLKNVRV
                     RKNHELEKSLELVDINEIVEKLKITNKVMKDYDEKIVRAISVLLTDVRNITIFNSEGN
                     AFTDKQERVRLVFQAIAMAPGAFEYSYNGPGAHKGFEYVEGLDLEKIARDVADVAVRK
                     LTAAECPSGKMPVVIGNGFGGVIFHEACGHPLEASSVSKGLSPFAGKVGKQIAHPAVT
                     AVDDGTIVNGWGSNNIDDEGVKTKKNVLIKNGILQSYLVDRFNGRRMGIESNGTGRRQ
                     NYTFEPTSRMSNTYIAAGKYTPEEIISSVKLGLYAKSLGGGQVNPATGDFNFACDEAY
                     IIRDGKVAELVKGASLVGNGADILMKIDMVANDLERAQGMCGADSGSVPVDVGQPTIR
                     VSEITVGGRGGALQ"
     gene            6022..7341
                     /locus_tag="PK612_00245"
     CDS             6022..7341
                     /locus_tag="PK612_00245"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOH94585.1"
                     /translation="MNVKKFFELAKEKGLEAAELVIQKNKGIEMSVYDESVEKYTLAS
                     TINVLARGIYKGKMGFAMSEKDDKTTAQYLVAQIKMSAGVSETEDLSIIFEGSPKYRK
                     KSFFNKELATISESTKLESLFTIEKALKKAHPLITSVSTVQYSEIESDYKLFNSYGLK
                     LTNKQNYFYYGTWVVAKEGDDVKSHVEVFLDNDYSKFDADTFVKKAADGVIEKLGGKP
                     CATKDYPVVFNPKMTASLLAAYLTSASADRVEKNTSLFADKLNERVASSKVTVLDAPL
                     TANLFYRYFDDEGVAKTNKPIIKNGILQTFFHNLSTATKMGVEPTGHGRNTGGRISVG
                     ISNITLKPGRNDFDTLIKDIKEGVFITELMGLHSGLNTTSGDFSLQAAGFMIRDGKVA
                     EPVNLITVAGNLVKLFNDVKAVANENELQLSSYTTSSILVKSLKIAG"
     gene            7464..>8001
                     /locus_tag="PK612_00250"
     CDS             7464..>8001
                     /locus_tag="PK612_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOH94586.1"
                     /translation="MKKKLLLLLPFLLLAGCKAREKPDIVVTSFVSYDAVTNIVGDKL
                     KVKNIVPWGSELHDFEPTPRDIEDINNAKLFVYTHPALETWVKDLVTNKNSFDMSIHS
                     DVEYACKVKKQEHHDHEHDHDHTLHYWTDPHIYIRVMNDLLTVLIDISPANETYFTEN
                     HNNYTARLLATSNDFRDFL"
BASE COUNT         2507 a         1380 c         1343 g         2771 t
ORIGIN      
        1 tcacattttg ggcttctgtt aggtcatatt ttgagtttct gttcgtttat atttcacatt
       61 taattgattt caactatccc aattactttt ttatttagtg ttactacaac taatattttt
      121 cgtatttttt tcagttgcac ttaaattttt acgcatttaa ataaatagtc gcgcattctt
      181 ttataaaatg attattcgac aatcattact tacttatttc aaaatcacaa tttataattt
      241 ctaaagtaag ggtttcaaga tatttccaaa aatagttttt acatttgcta agatccaata
      301 tatagttctt ttgttgtatg tgtgtctaca tttagggtat cagcacataa gcgatattat
      361 ttttgtccac ttattttatg atacatttca aataattctt ttaaagacac atcaactcct
      421 gcttcatgaa gttttttctt tcttttatta aatagttttt taatcatttt gtattccttt
      481 ctaaaaatac atctttttta atatttttaa aaactaatag tccttataga ataaaatatg
      541 tgtttgctat attaataaaa aagattatct attgaagagc gttctataat taccttataa
      601 ataatgagta tattattttc ttaaaatcaa actaacaatc gtctccgcgt gagcggtccg
      661 cggaaacatg tcaacacatt caatatgttc aacattatac ttctttgtta acttttttaa
      721 gtctcttgct aaagtcgcgg ggtcacatga tacatacatt attttaggcg gtagcgctct
      781 aagtaaagtg ttaacaaatt cgtcactcaa tcctttacgt ggtggatcaa cgatgacaac
      841 gctgaagtta tagttcttta tttctctagt taaaaaagca ccagcgtcat ctttaaagaa
      901 cttaacattt gtaatattat tttgtttagc gttcacttct gcgtctttaa cagcgtcttt
      961 aacaatttca acaccgataa cttgtttagc tttttttgct gctaaaatag caattgtgcc
     1021 tgtgccagaa taagcgtcta acactacatc atttttggtt attcccgcta gagttagtgc
     1081 tctttcgtaa agcacctcag cctggagtgt attaacttgg aaaaacgatt tacttgaaag
     1141 cgtaaattta agtccgtgta gctcttcatt aataaaacca gggccatata gtactttttc
     1201 ctttttacct aaaataacat tggtatcttc gctattgata ttttgaataa tcgttgtaat
     1261 atttgggcaa attgccacta aatcacgaca aaatgcgtct agatttggaa tttgctcatc
     1321 ttttgtaata agtacaacca ttacttcacg aagataatga cttacacgaa ccattacttt
     1381 tctaacttgc ccagtatgac ttttttcgtc ataaacttcg taattatatt tctcaattaa
     1441 tttacgaatt tctgcgataa ctgtgcttgc taattcgtca tctaaatgtg tcttctccat
     1501 cgcaattatt tcatgcgtat tatgtctaaa taaaccggta taaactttgc cgctctttct
     1561 tttgccaaac ggtaatactg ttttattacg atagaaatat gggttcttag cagtgtggat
     1621 cttttcaatt ttaaaatcaa tgccaccgat aacgcgtaag gctcgttcaa cttttgaaag
     1681 tttatattcc acttcttttg cgtaatcaag ttgggtaaat tgacaacctg caagatggat
     1741 tgtttccggg acaactggtg taatacgatc gctagacatt ttagtaaaac gaattatttt
     1801 accagtcgca aaattacgca ttaccttcgt tattttaatt tccgcttctt caccaataat
     1861 taagttatta atgaaaatag gaaaattatc gactttagct aggccaatcc cttcactagt
     1921 gtagtcgccc ccagttacga cataaatgtc attttcttta atcattattt cattaaattt
     1981 ttaatgtatt caacttcttc attaactttt aggcgtggga ataatggttc acccttatta
     2041 actttttccc cacctaaaac accatattta tttaatagat taatatcttg tttatcttct
     2101 tttactccaa gttgcttaaa taattctttg ggtgcgttta ctagtacagg ttgcagtaat
     2161 attgcgccaa taaaaaggtt atttgctaag tggttcatta ctgaatctaa ttcttctttc
     2221 ttaccttgag tgtttaattc ccacggttta gtgttatcaa tgtatttgtt tgatgcacta
     2281 ataattttaa aagcagtggt aatcgcttct gtcactttta aatcactaaa gtgttcgtga
     2341 tattctttaa cgacattaat cgctaagtct tcgatttcgg tgtcaaattc agttacttga
     2401 cctctataaa gcggaataac accatcacaa tacttttcaa tcatcgatac tgttctattt
     2461 aataaattgc cgaagtcatt tactaaatca acattaaagc gttcgacgaa ttgttcaggt
     2521 gtaaattgtc cgtcattacc aaacacaact tctcttgcta agtaataccg cacagcgtcc
     2581 acgccatatt tatcaataag cggcaacgga tcaataacat ttccttttga tttagacatc
     2641 tttccgtctt tcatcatcag tagcccatgg acaaaataac ggtctgcttg acgtaaccct
     2701 agtgctttaa gaatcattgg ccaataaatc gtgtggaagc gcgtgatatc ggcacctaag
     2761 atttgcacta tttcaacatc ttcactttgc caaaatttat ggaagagttc atcatcttca
     2821 cttaaatacc ctaatgccga aatataatta attaaagcat caacccacac atacattgtg
     2881 tggcgctcat tttctctaac aggcactccc cactcaatac tcgtgcgggt gacggcaagt
     2941 tcgcttagtc ctggtttaat aaatgtatta accatttcat ttttacgcga ttcaggcgta
     3001 atgaagtttg gcgtttcttc atatttcttt aataatcctt caacatattt atcggtttta
     3061 aagaaatatg actcttcact tgctaaatgc acgctccgat tacaatcggg acaaagtttt
     3121 tcttcaccaa cctgagttgt agtccaaaat gattcacacg gagtacagta ccacccttcg
     3181 taactcccaa gatatatatc accttgttct aaaagtttgg tgaaaatctt ctgcacagtt
     3241 gcaatgtgtt ttggatcagt cgtgcgaata aaatcatcat ttgttattgc taatttttcc
     3301 cagaggtctt taaatgtttt tacaatatca tccacgaact tttgtggcgt tttatttgct
     3361 tttgccgcgt tttccgctat tttttgcccg tgttcatcac ttccagttaa gaaataaact
     3421 tctttaccaa tcatccgatt atagcgcgct aaaatgtcac ttaaagttgt acagtaagcg
     3481 tgaccaatat gtaacttagc gcttggataa taaatggggg ttgttatgta aaaacgtttt
     3541 ttcatagtgt acctcttata tttgaaaaaa ctgccatttg atttaacagt ttttcagtaa
     3601 tcaatttact taagtccgta ttttttatta aatttatcaa cacgaccatc agcttgggta
     3661 aagcgttgtt tccctgtata aaacggatgg caatttgagc aagtgtcaac acggatttct
     3721 ttttttgttg ataatgcttc aaattcagta ccacagctaa cacaagtcac tgtgattttt
     3781 tgcgtctttg ggtgaattcc ttgtttcata atatctcctt ccgcctaggt taactcgatt
     3841 taacctaagt aagcaaaagt aataatatca ttattacagt tatttgacaa ccgaaaatat
     3901 tattcctgag ttctaataat ctcttttatg atattttatg aaatcaataa aaagttccca
     3961 aaacaagtac cctagtaaaa ttattacttg caacagatag ttgcatttta atatcatagt
     4021 aaacaataca atcttttttt gaagtgaagt gtaccccaaa tctttgataa caaaaaaggg
     4081 gtacacatca acatcaacat caacatcaat acaactcttt tttcttcttt taaatgaatg
     4141 ttatgaaata gtaatttttt gatgtttctt ctatatggta tgcactgtat cgctgcgctt
     4201 caatcaactc gttaattata ataatgaaat tactcaaagt tagatttttt gaatttgaac
     4261 gattattgca atcaaaaaca tagaaagcaa tgttgctttt acatgctttt tttgaaaatt
     4321 gacttttatc ataatctttt tttgcttcat aacttaattc atttatgttt ttttattctt
     4381 cattaatatt tctcctcctt tcgttaagtt acaacaaaac caataattgt taattttgtt
     4441 gctcatataa tttagcgtga tttttttagt gctttttaaa gaaatattaa tcttttttat
     4501 tagtagctgc ccttattcgt tacaatttga atattaaatt gttgtattat ctaaaattcc
     4561 ctaaaaaagc gcttttcccc atatttcctt catatttttg ttatcatttt taattaggag
     4621 gaagtacatt atgattacaa aaaaacttgc cctcgaagta ttaaatgtag cgcttagtac
     4681 cggtggagat ttcgctgaaa tatttttaga agaaacttac agcggtaact acactgtaga
     4741 aaacggtaaa acgcaagcgc ctgctacttc gttaacttat ggggcaggca ttcgtatttt
     4801 aaaaggatta caaagtgttt atggtcacac aagtgactta agcaaaaaag gtttattaaa
     4861 tcttgccact gcgttaagcg ctggctttga tggtcaaaga gttttaagtg ttgaaaaact
     4921 taaaaatgta agggttcgta aaaatcacga gttagaaaaa agtcttgaat tagtggatat
     4981 taatgaaatt gttgagaagt taaaaatcac taataaagta atgaaagatt atgacgaaaa
     5041 gattgtacgc gctatcagtg tattattaac tgatgttcgt aatatcacta tttttaatag
     5101 tgaaggtaat gcatttactg ataagcaaga gcgcgtaaga ttggtgtttc aagcaatagc
     5161 aatggcacca ggcgcctttg aatatagcta caatggacca ggagcacaca aaggctttga
     5221 atatgttgaa ggtttagatt tagaaaagat tgcgcgtgat gttgctgatg ttgctgtccg
     5281 taaattaacc gcagcggaat gtccaagcgg gaaaatgccg gttgtgattg gtaacggctt
     5341 tggtggagtt atttttcacg aagcatgtgg tcacccgctt gaagcaagtt ctgttagtaa
     5401 aggattatca ccttttgccg gtaaagtcgg aaagcaaatt gctcacccag cggttaccgc
     5461 agtcgatgac ggcacgattg taaacggctg gggatcaaac aatattgatg acgaaggcgt
     5521 aaaaacaaag aaaaatgttt taattaaaaa cggcatttta caaagttatc ttgttgatcg
     5581 ttttaatgga cggcggatgg gaattgaaag caatggtaca ggccggcgtc aaaattatac
     5641 ctttgaacca acaagccgga tgtcaaatac atacattgcc gctggtaaat atacacctga
     5701 agaaatcatt agcagtgtaa aacttggatt atatgcaaaa tcacttggtg gtggacaagt
     5761 taatccagca actggtgact ttaattttgc gtgtgatgaa gcttatatta ttcgtgatgg
     5821 taaagttgcc gaattagtta aaggtgcttc acttgttggt aatggcgctg atatattaat
     5881 gaaaattgat atggtcgcta atgaccttga acgcgctcaa ggcatgtgcg gtgccgatag
     5941 cgggagtgtt ccagttgatg ttggccaacc aacaattaga gttagtgaaa ttaccgttgg
     6001 tggtagagga ggggcattac aatgaacgtg aagaaattct ttgaattagc aaaagaaaaa
     6061 ggccttgaag ccgctgaact cgttattcaa aaaaataaag gtattgagat gagtgtgtat
     6121 gatgaaagtg ttgaaaaata caccttagcg tccacaatta acgttctagc gcgcggtata
     6181 tataaaggta aaatgggttt tgcaatgagt gaaaaagacg ataaaacaac tgcccaatac
     6241 ttagttgccc aaattaagat gagtgccgga gtttcagaaa cagaagactt aagtattatt
     6301 tttgaaggta gtcctaaata tcgtaaaaaa tcattcttta ataaagaact tgccactatt
     6361 agtgaaagca caaaacttga atcgctcttt accattgaaa aagcgttaaa aaaagcgcac
     6421 ccgcttatta cttctgtttc aactgttcaa tattccgaga ttgaaagtga ttataaatta
     6481 tttaattcat atggtttaaa actcacaaat aaacaaaact acttctatta tggaacatgg
     6541 gttgttgcta aagaaggcga tgatgttaaa tcgcatgtcg aagttttcct tgataatgat
     6601 tatagtaaat ttgacgctga cacatttgtg aagaaggcgg ctgatggagt tatagaaaaa
     6661 cttggtggta aaccatgtgc tactaaggat tatcccgttg tatttaaccc aaaaatgacc
     6721 gctagtttac ttgcagctta cttaacaagc gcaagtgctg atcgtgttga aaagaacact
     6781 tctttatttg ccgacaaact aaatgaaaga gtagcaagtt caaaagtaac tgttcttgat
     6841 gcaccgttaa cagctaatct cttctatcgt tattttgatg acgagggcgt tgctaaaaca
     6901 aataaaccaa ttattaaaaa tggtatctta caaacattct tccataactt aagcaccgct
     6961 actaaaatgg gtgttgaacc taccggtcac ggccgtaaca cgggcggaag aattagtgtc
     7021 ggtattagta atatcacctt aaaacctggg cgtaacgact ttgatacttt aattaaagat
     7081 attaaagaag gtgtatttat cactgaactt atgggtctcc actcgggact caacacaaca
     7141 agtggtgact ttagtttaca agcagcaggg tttatgattc gtgacggtaa agttgctgaa
     7201 cctgttaact taattaccgt tgctggtaac ttagttaaac tctttaacga tgttaaggcg
     7261 gtcgctaatg aaaatgaatt acaacttagt agctacacta cttcaagtat tcttgttaaa
     7321 tcattaaaaa ttgctggcta gtaataaagt atctttatct aaatttaaga ggcgtccttt
     7381 gtggcgcctt tgctttttat tagcgataac gctaagctgt gtgttataat tctttttgcg
     7441 aatcattcgc aaattgaagg attatgaaaa agaaattatt attactatta ccattcttac
     7501 ttttagcggg ttgtaaggcg cgcgaaaaac cagatattgt tgttacttcg tttgtaagtt
     7561 acgatgctgt aactaacatt gttggtgaca aactaaaagt taaaaacatt gtcccttggg
     7621 gcagtgaact tcatgacttt gaacctacac cacgtgatat tgaagatatt aataacgcta
     7681 agctatttgt ctatactcat cctgcgcttg aaacatgggt taaagacctt gtcactaata
     7741 aaaatagttt tgatatgagt attcattctg atgttgaata tgcttgcaaa gtaaaaaaac
     7801 aagaacatca cgatcacgaa cacgaccatg atcacacact tcattactgg acagatccac
     7861 atatatacat ccgtgttatg aatgatcttt taactgtgct tattgacatt tcgccggcaa
     7921 atgagaccta ttttacagaa aatcataata attacacagc gcgattatta gccacaagta
     7981 atgattttcg tgactttttg t
//
LOCUS       DAOSEX010000005         7678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_8225_length_7678_cov_3.274826, whole genome shotgun sequence.
ACCESSION   DAOSEX010000005 DAOSEX010000000
VERSION     DAOSEX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8225_length_7678_cov_3.274826"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PK612_00255"
     CDS             complement(<1..166)
                     /locus_tag="PK612_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015958133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94587.1"
                     /translation="MNMAKVTGKIVKVSGPLVVASGMKTAQVYDVVLVGDKQIIGEII
                     ELRDDLASIQV"
     gene            complement(173..475)
                     /locus_tag="PK612_00260"
     CDS             complement(173..475)
                     /locus_tag="PK612_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94588.1"
                     /translation="MDELVLFSNKSEAILFKAIGFLVHVVKDEEEITEILKELPKEVK
                     VIAYDTFFTSFFTEYTKNQKQLYPLYLPLPFNTEDEGKAISEMKESIRKSIGIDLL"
     gene            complement(479..1195)
                     /locus_tag="PK612_00265"
     CDS             complement(479..1195)
                     /locus_tag="PK612_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOH94589.1"
                     /translation="MNNVYLKGVLDAKISNLLTESDFATFANLSGMDRLNFFINNELV
                     STSIVTNFEALCKLALSDLKEEVSLYTKNDPLYVNYFFAHDVIKKDQGALRDFYNETY
                     EKALQRKEEALVNYLDYTHALLNILTLVRGKKRGDTTASLLANYLKQGVLSEETFSAL
                     VNGDTHTIYAYLKNNFNIDASESESTVELEEKLDEYLFNKLKILAMDSDFKPTLIYYI
                     KRKQLQIERLRNIVYTKRNG"
     gene            complement(1188..1799)
                     /locus_tag="PK612_00270"
     CDS             complement(1188..1799)
                     /locus_tag="PK612_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94590.1"
                     /translation="MKKPIFERITEEAKKEAALLVKETKSEASRILKAGKDAISAQNE
                     SELTEAKAKSVERVRQYKERNEKGLDNYKEQVRQEIVVSIFDEVVNKLANLEGEELLK
                     FVTNLIAKEKVKGDEVMHVSARNFKKFVTALSSKKDATNLDLLNKKDAKYNFTLSKEP
                     THVAEGFLLSTPQYDLIFDFKEIVINYQKKHEQRIYNELFKDE"
     gene            complement(1809..2282)
                     /locus_tag="PK612_00275"
     CDS             complement(1809..2282)
                     /locus_tag="PK612_00275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOH94591.1"
                     /translation="MTTGLVYALIGAALAAGLAGVGSAVGVSLGGKAAAGVISEKPEL
                     FGRVLILQALPGTQGIYGFLVAVLIMVKIGMIGGNPIALTNEQGLALFAAALPIAIGG
                     LASGIYQGKMAAASISMTGKNPAMSARGMTMTAMVETYAILALLVSVLMYIAVPV"
     gene            complement(2283..3377)
                     /locus_tag="PK612_00280"
     CDS             complement(2283..3377)
                     /locus_tag="PK612_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOH94592.1"
                     /translation="MREDEFETLTKNLENKVDCDIEELAVQNEEMVPTATKNNKLVKP
                     FESITNMFSVPNPNELDPNPAMSVWYWIIFGIMMGDMGYGLAMLIIFGLFLKLAKPKG
                     GLKDLATIFLYSSIPSIIFGILTGSFFGVSVDLLNIVGKWFGNPNLTSVVLQPVEDPL
                     PMLIFSLALGIAHIITGLVLKIINDARKRDIKSMLADAVSWILILVGLVIAVVVKNQI
                     VLYVAYGMIGIGAIFILLFSGRDKKGIIGKALAGLGGLYNVTSYLSDVLSYSRLLALS
                     LSTAVIAFTMNLLAGMVGGIPVVGILFAAIVYLIGHVFNFAMGLLSAYVHDGRLQYLE
                     FFGKFYEGGGYEFTPFSYKLKYVNAIKENE"
     gene            complement(3384..4190)
                     /locus_tag="PK612_00285"
     CDS             complement(3384..4190)
                     /locus_tag="PK612_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94593.1"
                     /translation="MIVPVKKIRLLTAISEQGKLIEALQHESKFMLFEHENDPSLPSD
                     INNELRAIERGIKALSKYKKKKFFAYHEVKQSEFNTIEAEGKALLSKINSALKTIENA
                     EKEIAELQAEIKTLTPYLNLSVATKPLSELVRTRIVIGQVPLNLFEDFAKEADEKGHI
                     FEESSRDHEFVYGAFGYEVVYESQALILLNSYRFTEVTLPLYDTKLKALLPELNDNLA
                     NNDKAILEAKDSLKALSNDEEKLRIYYDYYYNLALRKLVVTKTTERVAYV"
     gene            complement(4187..4495)
                     /locus_tag="PK612_00290"
     CDS             complement(4187..4495)
                     /locus_tag="PK612_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94594.1"
                     /translation="MSVLEKIVQAELDAKELLAKTREDNTKLLSDTHDELATLRAENA
                     LAIAREINALNKKADHEIEKLATTFAAKKETITNEIEKSAKANQSKLVDKVFKDIVEK
                     "
     gene            complement(4694..7135)
                     /locus_tag="PK612_00295"
     CDS             complement(4694..7135)
                     /locus_tag="PK612_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOH94595.1"
                     /translation="MEKNGQILVVSNNFNTKNAVKTALENDFILFFSTPSEMFNEVEK
                     HPELCGVIFELKDENIQSVEQFSRLYLKYPRLKLLVLASKEFYESIDEEARFGFWCME
                     TSTAADLLNKIYELKEYPLSTIEKISLVYAAFERAPFGIMIIPNDNLELALFNRKNDE
                     VLGYTGEEYKKLGLNKETHPDDLQRVAEIEKQAKLLGENSFNVTKRVIKKDGNIVWVE
                     QFISIIKLEKYEGFSRIVISWDVTKQKAVEDTLAESDRARATLLENLPGMAYRSLNDK
                     NWTMEFVSDGALALTGYRPEELIDNKTVSYYDLIVRVHRPRIRQAFDEATKSKKQFRQ
                     EYEIVTRNGTKKWVSETAKPVFDKKGNLLFYEGIITDINHMKKIEKQLKHFQQYDQKL
                     NLPNREYLIDKIQAKLDSKNYSGTIILVNLKESQRLYRSHGYQYMELLTASLVTKLKT
                     IETSRITLHYAEEDLFVFYAKYQMTREEIKKFYAELKSAIGHTISREQIRSGIGVTFL
                     HKSISIAEVYLQRARIASEFIDDENALISIHIYNDIMDQKVKREEHLRQELIAISYEP
                     GNEHKLRLMFQPIINLQTGKVRGFEALARFTSDKYGPIAPSEFIPIVENNRLIIAFGK
                     KIINLAVSFAKELLATGIHDCGIAINISVLQLLDPNFAQDLFARIESAGVPPEMIMIE
                     MTESIFSTNYEQLSEALAEIKDYGIDTAIDDFGTGFSSLARIERLNFNTVKMDRVFVT
                     PINAENAEQGIAQDIINICKKFNIQSLAEGIETKEQYDALKGLGCEFGQGYYMSRPLE
                     VDAAIEYIKNAKRKK"
     gene            complement(7391..>7678)
                     /locus_tag="PK612_00300"
     CDS             complement(7391..>7678)
                     /locus_tag="PK612_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOH94596.1"
                     /translation="GYQVPVGVFIIQEGDEVALDRYEGFPTLYYKKDFVVELISENGK
                     KTTINAFAYIMHEDRILGAPSEEYIRRVREGYKNFNFDEKILDEAIVFSAK"
BASE COUNT         2282 a         1514 c         1161 g         2721 t
ORIGIN      
        1 aaacttgaat acttgccaaa tcatcgcgta attcaatgat ttcgccaata atttgtttat
       61 caccaacaag cacaacgtca taaacttgcg cagttttcat cccgcttgct acaacaagcg
      121 gaccagatac tttaacaatt ttaccagtta cttttgccat attcatttcc tcttatagaa
      181 gatcaatccc aattgatttt ctaatacttt ctttcatctc acttattgct ttaccttcat
      241 cttccgtatt aaatgggagc ggtaaataaa ggggatataa ttgtttttga tttttagtgt
      301 actcagtaaa gaacgatgta aaaaaggtgt cataggctat tacttttact tcttttggta
      361 attcttttaa gatttcagta atttcttctt cgtctttgac aacatggact aaaaagccaa
      421 ttgctttaaa aagaattgct tcgcttttat tactaaataa cactagttcg tccatctttt
      481 atccgttcct tttcgtgtac acaatattgc gtaacctttc aatttgtaat tgcttacgtt
      541 taatgtaata aattaatgtt ggtttaaagt cactatccat cgctagtatt tttaacttat
      601 taaataagta ttcatctaac ttttcttcta actccactgt tgattcgctt tcactagcgt
      661 caatattgaa gttgtttttt aaatacgcat agatagtgtg agtatcacca tttactaaag
      721 cgctaaaagt ttcttcactt aacacaccct gttttaagta attcgctaat aaactcgcgg
      781 tggtgtcgcc tcttttttta ccacgcacta gtgttaatat gtttaagagc gcgtgcgtat
      841 aatctaaata atttactaag gcttcttctt ttctttgtag tgctttttca tatgtttcat
      901 tatagaaatc acgaagtgca ccttggtctt ttttaataac atcatgggca aagaaataat
      961 taacgtataa ggggtcgttt ttcgtataaa gactaacttc ttcttttaaa tcactaagcg
     1021 cgagcttaca tagcgcttca aagttcgtaa cgatacttgt cgaaacaagt tcattattaa
     1081 taaagaagtt aagtcgatcc ataccgctaa ggtttgcaaa agttgcaaaa tcactctctg
     1141 ttaagagatt tgatatttta gcgtcaagta ctccctttaa ataaacgtta ttcatcttta
     1201 aataattcgt tataaatcct ttgctcgtgt ttcttttgat agttaatgac gatttctttg
     1261 aagtcaaaga ttaagtcata ctgcggtgta cttagcaaaa agccttccgc aacatgtgtc
     1321 ggttctttac ttaatgtaaa gttatatttg gcatctttct tatttaataa gtcaaggttt
     1381 gtcgcatctt tcttacttga aagcgcggtc acaaatttct taaagttgcg cgcagaaaca
     1441 tgcattactt catcaccttt aactttttct ttagcaatta aattagtgac aaattttaat
     1501 aattcttcac cttctaaatt cgctaattta ttaacaacct cgtcaaagat gcttacgaca
     1561 atttcttgac gaacttgttc tttatagttg tccagtcctt tttcgttacg ctctttatat
     1621 tggcgaacgc gttcgacact ttttgcttta gcttccgtta attcggactc attttgcgcg
     1681 gaaatagcgt cctttcccgc ttttaaaata cggctggcct cgcttttagt ttccttaact
     1741 aaaagtgcag cctccttttt agcttcttca gtaattcgtt caaagattgg ctttttcata
     1801 agcgctcctt aaaccggtac cgcaatgtac attaagacac taacaagtaa cgctaaaatt
     1861 gcgtaagttt caaccatcgc tgtcattgtc ataccacgcg cagacattgc tgggttttta
     1921 ccagtcatgc taattgaagc agcagccatc ttaccttgat aaatgcctga agctagccca
     1981 ccaatcgcaa tcggtaaagc agcggcaaat aatgctaaac cttgttcatt agtaagagca
     2041 atcgggtttc cgccaatcat cccaatttta accataatta agacggcgac gaggaacccg
     2101 taaatccctt gtgtacctgg taaggcttgt aaaattaaca cccgaccaaa taattctggt
     2161 ttttctgaga ttacaccagc ggcggcttta ccacctaaag aaacacctac tgctgaacca
     2221 acgcccgcaa gacctgcggc aagagctgct ccaatgagcg catacactaa tcctgttgtc
     2281 atttattcat tctcctttat tgcattaaca tattttaatt tgtacgaaaa cggcgtaaat
     2341 tcataaccgc caccttcgta gaacttccca aaaaattcta agtattgtaa acgtccatca
     2401 tggacatagg cacttagaag gcccattgca aagttaaaga catggccaat taaatacaca
     2461 atcgccgcaa ataaaatgcc gacgactgga ataccgccaa ccatgcccgc taaaagattc
     2521 atcgtaaaag caataactgc tgtacttaaa ctaagcgcta ataaccgtga ataacttaaa
     2581 acatcactta agtaacttgt aacattgtat aatccgccta gaccagcaag cgctttacca
     2641 atgatccctt tcttatcgcg tccgctaaat aataagataa aaatagcgcc aatcccaatc
     2701 atgccgtaag ctacatagag cacaatttga ttttttacaa caaccgctat cactaatccc
     2761 actaagatta aaatccaact aacagcgtca gcaagcattg atttaatatc acgctttctc
     2821 gcatcattaa taatctttag tactaaaccc gtaataatat gagcaattcc taacgctagt
     2881 gaaaagatta acatcggcag cggatcttca acgggttgca gtactactga agttaagttt
     2941 ggatttccaa accatttacc aacaatattt aataaatcga cagaaacacc aaagaaactg
     3001 ccagtaagta tcccgaaaat aatcgaggga atacttgaat aaagaaagat agttgctaag
     3061 tctttaagtc cgcctttagg ttttgctagt tttaaaaata acccaaaaat aattagcatc
     3121 gctagaccat aacccatatc acccatcata atcccgaaaa taatccaata ccacactgac
     3181 attgcggggt taggatcaag ttcattaggg ttaggaacac taaacatgtt tgtgatcgat
     3241 tcgaatggtt tcactagctt attatttttt gtcgcagttg gcaccatttc ttcgttttgc
     3301 actgctaact cttcaatatc acaatcaact ttattttcta agttttttgt taaggtttca
     3361 aactcatctt cacgcaccca accttagaca taagcgacgc gttcagtcgt tttagttacc
     3421 acgagtttac gcagtgctaa attgtaataa tagtcgtaat aaatccgtaa cttttcttca
     3481 tcgttactta acgcttttaa actatctttt gcttctaaaa tagctttgtc gttattcgct
     3541 aaattgtcat ttaattccgg caaaagtgct ttaagttttg tatcatatag tggtagtgtt
     3601 acttcagtaa aacgatatga attaagtaaa atgagcgctt ggctttcata cactacttca
     3661 taaccaaaag cgccatagac aaattcatgg tcgcgtgatg actcttcaaa aatatgacct
     3721 ttttcgtccg cttctttagc aaaatcttca aataaattaa gcgggacttg tccaataaca
     3781 atccgtgttc gcactagttc actaagtggt ttagtggcaa cacttaaatt taagtacggc
     3841 gttaatgttt taatttctgc ttgtaattcc gcaatttctt tttcagcgtt ttcaattgtt
     3901 tttaaagcgc tattaatttt agaaagcaaa gctttacctt ccgcttcaat cgtgttaaat
     3961 tcactttgtt taacttcatg ataggcaaaa aactttttct ttttgtattt gctaagcgct
     4021 ttaatcccgc gctcaatcgc tcgaagttca ttattaatat cactaggaag gcttggatcg
     4081 ttttcatgtt caaaaagcat aaacttgctt tcgtgttgta atgcttcaat aagcttacct
     4141 tgttcactga ttgcggttaa tagtctaatt ttctttactg gaacaatcat ttttctacaa
     4201 tgtccttaaa gactttatcc actagtttac tttggtttgc ttttgcactt ttttcaatct
     4261 cattagtgat tgtttctttt ttcgcagcaa aagttgtcgc tagtttttca atctcgtgat
     4321 cagctttttt attaagcgcg ttaatttcac gcgcaattgc taaagcattt tcagcgcgaa
     4381 gtgttgctaa ttcatcgtga gtatcactta ataatttcgt gttatcttct cttgtcttag
     4441 cgagtaattc tttcgcgtca agttcagctt gtacaatttt ctctaataca ctcataggac
     4501 tcctttctag gcattttgtc cgctaccaat ttatttatat aaataggtag agttttgtat
     4561 ccacgtgtag tattttaaca tgtttacatt gctaatgtaa tgtgatacga ctatgtgctt
     4621 ccgctaaaat gttgaatgta tactaaaaaa ccgcactatg cggttatttt agataagaac
     4681 ataatagtta aaactatttt ttgcgtttag cgttttttat atattcaatt gccgcgtcaa
     4741 cttcaagtgg gcggctcata taataccctt gcccgaattc acagcccaag ccttttaggg
     4801 catcatattg ttctttagtt tcaatgcctt ctgcgagtga ctgaatatta aactttttac
     4861 aaatgttaat aatatcttgg gcaatacctt gctcagcgtt ttcggcattt attggtgtca
     4921 caaaaacgcg gtccatttta accgtgttga aattcaacct ttcgattctc gctaatgaag
     4981 aaaagccagt accaaagtca tcaattgccg tatcaatccc gtaatcttta atttcagcaa
     5041 gtgcttcact taattgttca tagtttgtag agaaaattga ttcagtcatt tcaatcataa
     5101 tcatttcagg tgggacaccc gctgattcaa tgcgtgcaaa aaggtcttgg gcaaaatttg
     5161 gatctaagag ttgtaatacg gaaatattaa ttgcgatacc acaatcatga atccctgttg
     5221 cgagcaattc ttttgcaaag gaaaccgcta agttaataat ttttttacca aaagcaataa
     5281 taagacgatt attttcgaca attggaataa attctgacgg ggcaataggc ccatatttat
     5341 cactagtgaa tcgcgcaagt gcttcaaagc cacgtacttt accagtttgc aaattaataa
     5401 tcggttggaa catgagtcgt aatttatgtt cattgcctgg ctcgtaagat atagcaatta
     5461 actcttgacg gaggtgttct tctcgcttca ctttttgatc cattatatcg ttgtagatat
     5521 ggatagaaat aagtgcattt tcatcatcaa taaattcact tgcaatccgt gctctttgta
     5581 agtacacttc cgcaatgcta atgctcttat gtaagaatgt tacaccgata ccagagcgaa
     5641 tttgttcgcg cgaaatagtg tgaccaatcg cacttttaag ttcagcgtaa aactttttaa
     5701 tttcttcgcg tgtcatctga tatttagcgt aaaatacaaa caaatcttct tcagcataat
     5761 gcagtgttat tctgcttgtt tcaattgttt ttaatttagt aacaagcgaa gctgttaata
     5821 attccatata ttgatagcca tgtgaacgat ataatctttg actttctttt aggttcacta
     5881 aaataatcgt tccgctataa tttttactat ctaattttgc ttgtatttta tcaattaaat
     5941 attcgcggtt tggtaaatta agtttttgat catactgttg aaaatgtttg agttgttttt
     6001 caattttctt catatgatta atgtcggtaa ttatcccttc atagaataat aaattacctt
     6061 ttttatcaaa tactggtttt gcagtttcac ttacccattt tttagtgcca ttcctcgtca
     6121 ctatttcata ttcttggcgg aattgctttt tactctttgt tgcttcatca aatgcttgac
     6181 gaattcttgg tctatgaaca cgcacaatta aatcgtaata ggaaacagtc ttattatcga
     6241 ttaattcttc aggtcgatac cccgttaagg caagcgctcc atcagacaca aattccattg
     6301 tccaattttt atcattaagt gagcgatacg ccatacctgg taaattttct aataaagtag
     6361 cgcgagcgcg atcactttcc gctagtgtgt cttcaaccgc tttttgtttc gtgacatccc
     6421 aagatattac tatccgtgaa aatccttcat atttttctaa ttttattatc gaaataaatt
     6481 gttcaaccca tacgatgtta ccgtcttttt ttataacgcg tttcgtgaca ttgaaactat
     6541 tttctccaag tagtttagct tgtttttcaa tttccgctac tctttgcaaa tcatcaggat
     6601 gagtttcctt atttaatccc aactttttat attcttcgcc tgtataacca agaacctcat
     6661 cattttttcg attgaaaagc gctagttcta aattatcgtt ggggataatc atgatcccaa
     6721 acggtgcacg ttcaaaagca gcgtacacca gtgatatttt ttcaattgtg gaaagcggat
     6781 attcttttaa ttcatatatc ttatttaata agtccgcagc ggtgcttgtt tccatacacc
     6841 aaaagccaaa acgtgcctct tcatcaattg actcataaaa ttctttactc gctaacacta
     6901 atagtttaag gcgaggatac tttaaatata gacgactaaa ctgctcaacg ctttgaatgt
     6961 tttcgtcttt caattcaaaa ataacgccac agagttcagg gtgtttttca acctcattaa
     7021 acatctctga tggcgtacta aaaaataata tgaaatcatt ctcaagcgca gttttgactg
     7081 cgttcttggt attaaaatta ttactgacaa ctaaaatttg tccgtttttt tccatttgac
     7141 cctgtttcta ttatacatta taacatctgt tatacatatt tttttgtttt ttgaaaaaaa
     7201 taaatattat tgagtttttt cggtgatttt tcgctcctta aaatttttaa aaatttaaaa
     7261 aaattgcaat ataaaaatta ataaaatata taaatgtttt gcattttctt tagcaacata
     7321 tactcaaaaa tcataataat attaaaagtt ttatcttgca aaacaatgtt atttcccttg
     7381 catttcgtgg ttattttgca ctaaatacaa ttgcttcatc taatattttt tcgtcaaagt
     7441 taaagttttt atatccttcg cgtactcttc ttatatattc ctcactagga gcgccaagaa
     7501 tcctgtcttc atgcatgata taggcaaagg cattaattgt tgttttcttt ccgttttcac
     7561 ttattaattc aacaacaaaa tcttttttat agtaaagtgt cggaaatcct tcataacggt
     7621 caagcgctac ttcgtcacct tcttgaataa taaaaacacc aactggcact tgataacc
//
LOCUS       DAOSEX010000006         7118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_9185_length_7118_cov_3.331304, whole genome shotgun sequence.
ACCESSION   DAOSEX010000006 DAOSEX010000000
VERSION     DAOSEX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9185_length_7118_cov_3.331304"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PK612_00305"
     CDS             <1..529
                     /locus_tag="PK612_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOH94597.1"
                     /translation="ATETLDISSDLDELARTNIAVVCAGPKAILDIEKTLEYLETKGV
                     PVLGYKTDKMPVFYTTSPSLNVLYRVDSSSEIASIVNASHALGLESGILVVNPIPKEK
                     ALPYKDIEKVIDEALEEMKKAGVKGKEETPYLLSKLNELTKGKSQAANEALVINNAKL
                     GASIAKHICRELYED"
     gene            519..1064
                     /locus_tag="PK612_00310"
     CDS             519..1064
                     /locus_tag="PK612_00310"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOH94598.1"
                     /translation="MKTKARLGNSLKKFMATMPLDTISVINITKDCKVNRQTFYYHFR
                     NIYDLLTWVFLNEEIEKPSNNELEEAIFIVLRYVKHNEAMIENTLASAGKDLVREFLF
                     NYLYNVCLNYIDKKDPFEKLTLNERKLLARVYGAGIASSIISWIEGEYLFPEHEFVMN
                     LVTFFNDFPLDAKNYKGHGYA"
     gene            1057..2229
                     /locus_tag="PK612_00315"
     CDS             1057..2229
                     /locus_tag="PK612_00315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOH94599.1"
                     /translation="MHSFNLKIQTELIFGNGRHTEVGSIIKRYGKKNVIILYGQNHAV
                     RSGLLEAVTNALDAENIDYVLYGGITPNPEVFYVREAKAIAKEYAVDFILAIGGGSVI
                     DVAKSLSVNFYYEGDALDFSKRIVTPKKALPLGVILTHASAGSEMSASCVISDSTNNF
                     KQGFRNDLVRPLFAITNPELTYSVSMYQTAVGIVDTFMHSLERYFNKSDKIELADRFA
                     EAIFVTLRECAETLIADPLDETARANLMLASTFSHNDVTSMGKKANLVVHTLEHALSA
                     LYPSIAHGHGLAVLYPAWLFVYFEELKEKLDRFARVVFNLHHEDIDTNAKEGILALKE
                     LFMRLGLTTTLSSLGIKREDIPTLADIVTENGTKELYHDQKNLTRYDIIKLYESCF"
     gene            2248..3369
                     /locus_tag="PK612_00320"
     CDS             2248..3369
                     /locus_tag="PK612_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94600.1"
                     /translation="MKKEDLLSILVYLVMLIIALFIGLRIIQPALDALDLVTDLQRYG
                     FAILTIFVGIIINVILFELGHVFGALLGGYSVISVNILGLAYYKTKSGWKFSFRRYEG
                     LTGETKIIAKSDKTKPRLYLFGPTIMVLLEFVIAIVIFLLTKDNQPIHHQVLIVAGIG
                     AMLLVYNIMPFKLDNFTDGYYIVLLGKKVNVEAYNELIRIESLVYNNEKVTDIKEFDE
                     VTTMTARLSLYRLYQLIDEKAWDKALSLIDDLEKNASKVEHEFIARIRAQKLYIYLLT
                     KASEAASAYWFDELSAADRKFISNDLTIETMRVYILYSGLVTKSQSECAFVLSRAPKA
                     LRARINDFRKEEEIALFNEAYEKIVALPGNENLKLPVLK"
     gene            complement(3427..3930)
                     /gene="mscL"
                     /locus_tag="PK612_00325"
     CDS             complement(3427..3930)
                     /gene="mscL"
                     /locus_tag="PK612_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOH94601.1"
                     /translation="MRKFFKEFKAFIARGNVLNLAVGIIIGGAFNAIITSLVNHVIMP
                     PISLLLGNEVSLLKWVIRPEVRDAADNVVTAEIAISWGLFVQAIINFLLIALVLFVIV
                     RVVTRLNEKAEALRAKLLHKEAAAAPEPEPEPEPSEEVLLLREIRDSLVAKKQEAKAT
                     KKKEPKK"
     gene            4106..4960
                     /locus_tag="PK612_00330"
     CDS             4106..4960
                     /locus_tag="PK612_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH94602.1"
                     /translation="MPKFKIVVDSTIDLSPEQYKKLGAIVTPLNVHIGEETFQDGVDI
                     NADKLYEKVAETKTAPKTSAVAPGVFKEVFEKLKAEGYTGALFVGIGEFLSATAQSAR
                     LAAEEVKDFKVRVVDSHSLSTGSGLLALRAYDLLNEGKTLDETADYLEKIAPKSRAQF
                     IVDNLEMLAAGGRVTGIKLFFGRILRAHPFLQVNNDKLEVVATPKGKSERALDLMLQV
                     AREEISAGLESPRVFITHSHGGERIDYLYDRLKEFVDPKNILISEAGAVISSHCGAGT
                     IGILYIRK"
     gene            complement(5005..5382)
                     /locus_tag="PK612_00335"
     CDS             complement(5005..5382)
                     /locus_tag="PK612_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOH94603.1"
                     /translation="MRLTPARKLVLNLFKTKSSLLCAEDIERLLKNESIFLSTIYRAL
                     EYFEKENVLGKSTINKTAYYYLIDGKHHHYMICTTCLARFPIDCKLDSMINKNMKKHN
                     FVPTSHDLTIYGYCENCAKLQNN"
     gene            complement(5360..6199)
                     /locus_tag="PK612_00340"
     CDS             complement(5360..6199)
                     /locus_tag="PK612_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOH94604.1"
                     /translation="MFSFLQHTFMVYALLLALLIALAAALLSPFLVLSKQSLIADGMA
                     HIAFTGVVIGLLFSNEPLYIALPFALLASIAITFLTERANLDNDAAIGVVSVFALALG
                     LIIISKSDGFNRSVESLLVGSLLTVTVPEIIGASVLALLVICFILIFYRKLIITTFDV
                     THARFDGVNTMFLKYALSALTSIFVVVGVRAIGALLISAFILFPTLISRQLSKSFNMT
                     LWGGVIISLIATFIGITVSYHFTLPTGPAIIMVFTTFLVVAYIIRIIIAKRRLKDAPN
                     ARS"
     gene            complement(6192..6899)
                     /locus_tag="PK612_00345"
     CDS             complement(6192..6899)
                     /locus_tag="PK612_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOH94605.1"
                     /translation="MPTSSRKMSQTSNKPLAVKLTDVSVNFGDVIALKNINFSINKGM
                     LLYVIGPNGSGKTTFIRLLAKLLEQTHGTIEHSDDRLGYLPQKLNINPNFPITVEEVI
                     YSGLAKQSLLISKGDRELIKSWLKRMQIPHLLKKPMAHLSGGQQQRVYIIRALISNPD
                     FIILDEPTSALDPSFRSFFNDIITALHHEGKTIIFVTHELHGALCDCASVLYIDQEIR
                     FFGGLQEYFEKEKNIHV"
     gene            complement(6844..>7118)
                     /locus_tag="PK612_00350"
     CDS             complement(6844..>7118)
                     /locus_tag="PK612_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94606.1"
                     /translation="KPDVDFLSQEIIDFMYEIKTFDSHYLFIEELVEPRVANLIKNEL
                     AKENYAITLLELHGYHNITAAQAKEGITYADIFAQNVANIKQAFGG"
BASE COUNT         2455 a         1167 c         1344 g         2152 t
ORIGIN      
        1 tgcgactgaa acactagata tttctagcga ccttgatgaa cttgccagaa caaacattgc
       61 ggtcgtatgc gctggtccaa aagcaattct tgatattgaa aaaacattgg aatatttaga
      121 aactaaaggt gttccagttt taggttataa aaccgacaaa atgccagtat tttacacaac
      181 aagtccgtcg ttaaacgtct tatatcgcgt tgattcttct agcgaaattg cttcgattgt
      241 taacgcaagt catgccttag ggcttgaaag cggaatactt gttgttaacc caattccaaa
      301 agaaaaagcc ttgccttata aagacattga aaaagtaatt gatgaagcgc ttgaagaaat
      361 gaaaaaagct ggggttaaag gtaaagaaga aacgccttac cttctttcca aactaaacga
      421 attaacaaaa ggtaagagtc aagccgcaaa cgaagcactt gtcattaata acgctaagtt
      481 aggagcgtca attgctaaac atatttgccg ggagttatat gaagactaaa gctcgtttag
      541 gaaattcttt aaaaaagttc atggcaacaa tgccacttga tacaataagc gttattaaca
      601 tcacgaaaga ttgtaaagtt aatcgccaaa ctttctatta tcattttcgt aatatttatg
      661 atttattaac atgggtattt ttaaatgagg aaattgaaaa accatctaac aatgaattag
      721 aagaagcaat ctttattgtg ctgcgttatg ttaaacataa cgaagcaatg attgaaaaca
      781 ctttagcgag cgctggtaaa gatttggtaa gagagtttct ttttaattat ttatataatg
      841 tgtgtttaaa ttacattgat aaaaaagatc catttgaaaa attaacatta aacgaacgaa
      901 aacttttagc aagagtatat ggcgcaggta tcgctagtag cattatttcg tggattgaag
      961 gcgaatactt attccctgaa cacgaatttg tgatgaattt agtaacattt tttaatgact
     1021 tcccgcttga tgctaaaaat tataagggac acggctatgc atagttttaa cttaaagatt
     1081 caaactgaac tcatttttgg taacggccgg catactgaag taggcagtat aattaagcgt
     1141 tacggcaaaa agaatgtaat tatattatat ggtcaaaatc acgcagtaag aagtgggctt
     1201 ttagaagcag tgactaacgc acttgatgct gaaaatattg attatgtact ttatggcggg
     1261 attacgccta atccggaagt cttttatgtg cgtgaagcca aagcgattgc aaaagaatat
     1321 gcagtagact ttattttagc aattggtggt ggcagtgtaa ttgatgtcgc aaagtcacta
     1381 tcagtgaatt tttattatga aggcgatgca ctagatttta gtaaacggat agttactcct
     1441 aaaaaagcat taccgcttgg tgtaatctta acccatgctt cagcgggtag tgaaatgtcg
     1501 gcgagctgtg taattagtga tagcactaat aattttaaac aagggtttag aaacgattta
     1561 gtaagaccgc tttttgctat tactaatcca gaattaactt atagtgtctc aatgtatcaa
     1621 acagcagtgg ggattgttga tacatttatg cattcgcttg aacgctattt taataaaagc
     1681 gacaaaattg agttagccga ccgctttgca gaagcgatat ttgttacttt acgggaatgc
     1741 gcagagacac ttattgctga cccacttgat gaaacggcgc gcgctaattt aatgttagcg
     1801 agcactttta gtcacaatga tgtaacaagc atgggtaaaa aagctaattt agttgtccat
     1861 actttagaac atgcacttag tgcgctttat ccaagtattg ctcatgggca cggtttagcg
     1921 gtgctctatc ccgcatggct ctttgtttat tttgaagaat taaaagagaa actcgatcgt
     1981 tttgcgcgtg ttgtttttaa tcttcatcat gaagacattg atacaaacgc aaaagaaggc
     2041 attttagcac ttaaagaatt atttatgcgt ttaggattaa caactacttt atctagttta
     2101 ggtattaagc gtgaagatat ccccacttta gcggatatcg taacggaaaa cggcacaaaa
     2161 gagttgtatc acgaccaaaa aaatctcaca agatatgata taattaaact ttatgaaagt
     2221 tgtttttaaa actgttggag gacgaatatg aaaaaagaag atttgcttag catattagtg
     2281 tatcttgtca tgctaattat tgcgttattt attgggttaa gaattattca acctgcgctt
     2341 gacgcacttg atttagtgac tgatttacaa cgctacggat ttgcgatatt aacaatcttt
     2401 gttggcatta ttattaatgt tattttattt gaattaggcc atgtctttgg tgcgcttctt
     2461 ggtggctata gtgtgataag cgtaaacatc cttggattag cttattacaa aacaaaaagc
     2521 gggtggaaat ttagctttag acgttatgaa ggattaacag gcgaaacaaa aattattgct
     2581 aaaagcgata aaacaaaacc acggctttat ttgtttggtc caacaataat ggtgctttta
     2641 gaatttgtga ttgcaattgt tattttctta ttaacaaagg ataatcaacc gattcatcac
     2701 caagttttaa tagttgcggg gattggtgca atgcttctag tctataacat catgcctttt
     2761 aaacttgata acttcacaga cggatattac attgtgcttc ttggcaaaaa agtaaatgtt
     2821 gaagcgtata acgaattaat tagaattgaa tcgctcgtgt ataacaatga aaaagttacg
     2881 gacattaaag agtttgacga agtcacaaca atgacagcac gtttaagttt atatcgctta
     2941 tatcaattaa ttgatgaaaa agcgtgggac aaggcattat cattaattga tgacttagaa
     3001 aagaatgcga gtaaagttga acatgaattt attgcgagaa ttagagcgca aaaactttat
     3061 atttacttat taacaaaagc tagtgaagca gcaagtgctt attggtttga tgaactaagc
     3121 gccgcagatc gtaagtttat tagtaacgac ttaacaattg aaacaatgcg tgtgtatatt
     3181 ctctatagcg ggttagttac taagagccaa agtgaatgcg cctttgtttt aagtcgtgct
     3241 cctaaagcac tgcgcgctag aataaacgat ttccgtaaag aagaagaaat agcgcttttt
     3301 aatgaagctt atgaaaaaat tgtggctttg cctggtaatg aaaatctaaa acttccagta
     3361 cttaaataaa ataaaaaaca ccagtaaatg tttacttaga aactagaaac tggtgttttt
     3421 cttttactac tttttaggtt ctttcttttt agttgctttt gcttcttgtt ttttggccac
     3481 taatgagtca cgaatttcac gaagtaataa tacttcttcg cttggttctg gttctggctc
     3541 tggctctgga gcagctgcag cttctttgtg aagaagttta gcgcgtaaag cttctgcttt
     3601 ttcattaagt ctagttacga cacgaacaat cacaaataaa acaagagcaa ttaataagaa
     3661 gttaataatt gcttggacaa ataaacccca tgaaatagca atttctgcag tcacaacatt
     3721 gtcagcagcg tcacgaactt ctggcctaat aacccatttt aatagtgaaa cctcattacc
     3781 aagtaataac gagataggcg gcataattac atgatttact aaactagtaa taattgcgtt
     3841 aaacgcgcca ccgatgataa taccaacagc aagatttaag acattaccgc gggcaatgaa
     3901 cgctttaaat tctttaaaaa actttctcat aatttatttc ctttctgctt tcaattttag
     3961 cataaagcta aaagtaagta gtaaaacaaa taagaccgta gtactttaaa tatgcaaaat
     4021 aaaataaaat aagatattat tgatagtgaa gtcacttaaa agtgatgatt aaagagttgt
     4081 gagcaaatca caaaaaggag aaaatatgcc taaatttaaa atagtcgttg attcaacaat
     4141 tgatcttagt cctgaacaat ataaaaagct tggggcgatt gttacaccat taaacgttca
     4201 tattggtgaa gaaactttcc aagacggcgt agatattaac gctgataaac tttatgaaaa
     4261 agtagcagaa acgaaaacag caccaaaaac gagcgctgtt gcccctggag tatttaaaga
     4321 agtgtttgaa aaattaaaag ccgaaggtta tacaggcgct ttatttgtgg ggattggtga
     4381 attcctaagt gcaacagccc aaagtgcgcg tttagcagca gaggaagtta aagactttaa
     4441 agtccgagtc gtcgattcac actcactttc aacaggaagc gggttattag cgctacgcgc
     4501 ttatgacctt cttaacgaag gcaaaacact tgatgaaacc gctgattact tagaaaaaat
     4561 cgcgcctaaa tcaagagcgc aattcattgt tgataattta gaaatgctcg ctgctggtgg
     4621 aagagtaaca ggcattaagt tattctttgg tcgtatacta cgtgcccacc cattcttaca
     4681 agttaataat gacaagttag aagtagtagc gacaccaaaa ggtaaaagtg aaagagcatt
     4741 agacctaatg ctacaagtag cacgcgaaga aattagtgct ggcttagaat caccacgcgt
     4801 ctttattacg cactcacacg gcggtgaacg cattgattat ctttacgacc gactaaaaga
     4861 atttgtcgat cccaaaaata ttttaattag tgaagcaggc gccgttattt caagtcattg
     4921 tggcgcaggc acaattggta ttttatatat tcgtaaataa caataattaa ttactaatga
     4981 aatgccgctt aatcgcggca ttttttagtt gttttgcaac tttgcacaat tttcacaata
     5041 cccgtaaatt gttagatcgt gacttgtggg aacaaaatta tgtttcttca tatttttgtt
     5101 aatcatacta tctaatttac aatcaatagg gaatcgcgca agacaagtag tgcaaatcat
     5161 gtaatgatga tgtttaccgt ctattaagta atagtaagct gttttattaa tagtgctctt
     5221 gcctagaaca ttttcttttt caaaatattc taaagcacga tagattgttg aaagaaaaat
     5281 agattcattt tttaataatc gttcaatgtc ttcggcacaa agtaatgaag attttgtttt
     5341 aaataaatta agcacaagtt tacgagcggg cgttaggcgc atctttaagt ctccttttag
     5401 caatgataat acgaataata tacgctacta cgagaaatgt agtaaatacc ataatgattg
     5461 cgggtcctgt cggcaaagta aagtgataag aaacagtaat cccaataaaa gtagcgatta
     5521 gtgaaataat aacgccgccc cacaaagtca tattaaaact tttagataat tgtctagaga
     5581 ttaatgttgg aaacaaaata aatgcagaga ttagtagtgc cccaatcgcg cggacaccga
     5641 caacaacaaa aatagaagta agtgcggaaa gggcatattt aagaaacata gtattaaccc
     5701 catcaaaacg cgcatgagtt acatcaaaag tcgtaattat aagcttacga tagaatatta
     5761 agataaaaca aataacgagc aaagctaaaa ctgaagcacc aataatttca ggaacagtaa
     5821 ctgttaataa actaccaact aatagcgatt ccacagagcg attaaaacca tcgcttttac
     5881 taataataat taaaccaagc gctaaagcaa agacgctaac aacaccaatt gctgcatcat
     5941 tatctagatt agcgcgctct gttaaaaagg taatggcaat tgaggctaaa agcgcaaaag
     6001 gtaaggcaat ataaaggggt tcattactaa atagtaatcc aataacaacg ccggtaaagg
     6061 caatatgcgc catgccgtcg gcaattaaag attgtttaga taaaacaaga aacgggctta
     6121 aaagagcggc cgctagggca ataagcagcg ctaacaaaag agcataaacc ataaaagtgt
     6181 gttgtaagaa actaaacatg gatatttttc tccttttcaa aatactcttg taagccgcca
     6241 aagaaacgta tttcttgatc aatatataac acactagcgc agtcacaaag cgcaccatga
     6301 agttcatgtg tgacaaaaat aattgttttt ccttcgtgat gtaatgctgt aattatgtcg
     6361 ttaaagaaac tgcgaaagga cgggtcgagt gcagaagtgg gttcatctaa aataataaaa
     6421 tcaggattac taataagcgc gcgaataata tagacgcgtt gttgttgacc gccagaaaga
     6481 tgggccattg gttttttaag caagtgcggg atttgcatcc ttttaagcca tgattttatt
     6541 agttctcgat cacctttact aatcagtagt gactgtttag caaggccgct ataaattact
     6601 tcttcaaccg tgattgggaa attagggtta atatttaatt tttgcggtaa ataacctaaa
     6661 cgatcatcac tatgttcaat agtgccatgc gtctgctcta acaatttagc aagcaacctt
     6721 ataaaagttg ttttacctga tccgttagga ccaatcacat aaaggagcat ccctttatta
     6781 attgaaaagt tgatgttttt aagcgctatg acatcaccaa aatttacact tacatcagtg
     6841 agtttaaccg ccaaaggctt gtttgatgtt tgcgacattt tgcgcgaaga tgtcggcata
     6901 tgttataccc tcctttgctt gtgctgcagt aatattgtga tacccatgaa gttctaaaag
     6961 agtaatcgca taattttcct ttgctaattc atttttaatt aaattagcga cacgcggttc
     7021 cactaattct tcgatgaata agtaatgcga atcaaatgtt ttaatttcat acataaaatc
     7081 aataatttct tgtgataaaa aatcaacatc tggtttat
//
LOCUS       DAOSEX010000007         6986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_9402_length_6986_cov_3.314096, whole genome shotgun sequence.
ACCESSION   DAOSEX010000007 DAOSEX010000000
VERSION     DAOSEX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9402_length_6986_cov_3.314096"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PK612_00355"
     CDS             <1..330
                     /locus_tag="PK612_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94607.1"
                     /translation="PAYIIWWFILYGIGAAINLAITQDINYFIQTYLFTLFDFLLGFY
                     LFTTLMSLLISVSNWKRIKMHPLKKIMLPFVAFFFLLSYVPMLLIAPFVKVTWRPVKH
                     YGLKKPL"
     gene            complement(325..1302)
                     /gene="fni"
                     /locus_tag="PK612_00360"
     CDS             complement(325..1302)
                     /gene="fni"
                     /locus_tag="PK612_00360"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOH94608.1"
                     /translation="MSRRRKDEHIAIAKIAKIRKNDFSHIRFIHHSIPSLSLKDIDLS
                     TSFLGHKFNYPFYINAMTGGAKKGDKINAKLAILAQHFGLPLFVGSQSLAFKNKESRL
                     AFETLRFNFPDLFIVANINPNFTAEMASDAVEILGANALAIHVNSVQELVMGEGDRDF
                     TKWKDNIANILKTVKVPVIVKEVGYGMHEPTLLLLQELGVKHIDISGMGGINFSLIEL
                     KRQKKKDSPLLDFGISTVDALLLARNYPNFEVLASGGINNASSIVKSLAMGAKATGMA
                     KYFLDLCNLPIPEMIKKVDELIIDLKKILVLLNVKSVRELNLDHLMFDL"
     gene            complement(1295..2272)
                     /locus_tag="PK612_00365"
     CDS             complement(1295..2272)
                     /locus_tag="PK612_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94609.1"
                     /translation="MRVHEKVPGKLYVIGEYNVLKSGHGAIVAPVNLFLQGTFVANHK
                     TTIIEDGVAYPYQTLLENGCPHQLKNTVTTITFFNEYLALKNIPLQQFEYTIENNLIS
                     DEQVKFGLGSSAASVVLTLKLLNRLYRTNLSPLVLFKMAVLIQRELNALTSGGDLACT
                     IFDMPLYYVRYDLNWLLNEKRTFELLEVAWPLLEIKLLTKLPTFLVGWTKETITPDTN
                     GTIDSAFFKKANKLVHHYLVTYDDIYIKQYQNLLDKLGLTRQGMITPRLEALVKTARS
                     LGVAAKISGAGYGDCGIAQINDQSVVEPLTNLWENEGIIVLDIWRKDNE"
     gene            complement(2259..3212)
                     /gene="mvaD"
                     /locus_tag="PK612_00370"
     CDS             complement(2259..3212)
                     /gene="mvaD"
                     /locus_tag="PK612_00370"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOH94610.1"
                     /translation="MKYKKANVNIAILKYWGKVNALINIPYQTSISVTADNFYTLTNV
                     ELDPKATKDIVILDGTALSGISYTRVAHHLDALRKHFKRKEYCIVNSINRVFTKAGYA
                     SSASGFAALTAAYIDAIGAKVSKKEMSKLARLGSGSATRSIHGGFVIWHRGNSHDTSY
                     AEKLQVKWPEFRMLFTIIDPSVKKISSRVGMQITIEKAKSYADYVKQSNESVEPLITA
                     LKENDIDAVGRIAERAAELMRNVMLEAGLEYHTPKTQQLIQKVKAIRSKHKIPVYYTF
                     DAGPNLILFTLDEYVNEVIRLLPEVKISVSGVGGGITSARS"
     gene            complement(3212..4102)
                     /gene="mvk"
                     /locus_tag="PK612_00375"
     CDS             complement(3212..4102)
                     /gene="mvk"
                     /locus_tag="PK612_00375"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOH94611.1"
                     /translation="MDGYGLAKIILIGEHSVVYGHPAIAIPFYAMRSEVVLTPHEEFI
                     LDTKYYTGPLNNAVDELRGLVALITHMAEIYKKIKPVIISVRSSLPEKSGLGSSASVA
                     KAVIDAFNNHFHLELTHIDYFELLKISENIYHHNASGIDASTVIYEKPIIYQRPHIKP
                     LKFNIDGYLAVFYSNTNSATKSAVAHVSTHPERDIHIDALGSLTANAKWALENNDITL
                     LATMFNAAHTHLRALGISTPTLEELRTKLLDAGALGVKITGGGMGGCLIALFNDEEKA
                     LATKEKLKSFPSWMIDLRNM"
     gene            complement(4116..5507)
                     /locus_tag="PK612_00380"
     CDS             complement(4116..5507)
                     /locus_tag="PK612_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOH94612.1"
                     /translation="MVITFSLILQMVGIALLMTALYLIIGIIPGTDETSVLVPMTLIL
                     VTLKLNPLVIMAGFIAAVVTLNIADSIPTAITSIPGGVMSTPLVGHSQTLKKHGLTSY
                     SVQWMMLGSLIGVLVSFPIALAFWGIFEFISVQTGLEISNFIKRYNEYIFLGGAILMS
                     LTSKEKVVSLLSIVPFGFATAFLRNPGLIINNTLLNTFIGGRTLSMTPFFLSITTGPL
                     VFALFELFFPSVFKNQKVEGKMTVILDDDVKKTKHGHKKIIAKTGIASALGSLLFFMS
                     PVGVTMMLGEFATKDEKDEVQQALQGVSAVNGLSSATYLAGIMISLFVFAFPISPAAL
                     GPGSVIFSPGGPIILEGNAGLAVPKGYLLIAIVIGLVIGLSLTVWLGMKYSHKMTELV
                     FKYISQETVLIFLFSIIALLSFAEAGLYGLIIVVSVGLLSGWLNKKGAGYGVQFMALY
                     AGGLFITILKALF"
     gene            complement(5511..5795)
                     /locus_tag="PK612_00385"
     CDS             complement(5511..5795)
                     /locus_tag="PK612_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94613.1"
                     /translation="MKKKKTMVRLWGDHIVISSLFIGLAISLAFVALTFVTIEVLAKH
                     VAIIAEKKKDFIYGFGTLAILVAFVINNFWIKPQRNIVKSSDLNKKEREG"
     gene            complement(5961..>6986)
                     /locus_tag="PK612_00390"
     CDS             complement(5961..>6986)
                     /locus_tag="PK612_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH94614.1"
                     /translation="QVTKQQPGLLIGFIVVLFFVLVAMGIYRSPAVSLMPDVTPKPLR
                     SKANAVINFMGTMGGAFAYVLVFLLASGPIFDKTGYVATFATTSLLMLLLIAIFVATV
                     KENAWYKEMVEITEKYHLDSLDIDDEGLDVSQHSDDEKMPKDVRRSFILILSAVVLWF
                     MGYNAATTKFSVYAFNHLGLEQFSLPPLIGIITAALAFIPIGYISQKIGRRRMILFGI
                     VVLTIALGSAVFITDETKVLMYIIMGLVGISWASINVNSYPMVVEMSRHGNIGVYTGY
                     YYSASMSAMIITPILSGALMDITGWYPVLFIYSAVFMGLAFIPMYFVKHGEPAPVVLK
                     EKAQKDE"
BASE COUNT         2350 a         1339 c         1148 g         2149 t
ORIGIN      
        1 ccagcttaca tcatttggtg gtttatatta tatgggatag gtgctgctat taacttagcg
       61 atcactcaag acattaatta ttttatacaa acttatcttt ttacattatt tgatttctta
      121 ttagggtttt atctttttac tacactaatg tcgttattaa ttagtgtttc aaattggaaa
      181 cggattaaaa tgcatccact taaaaaaata atgctaccgt ttgtagcatt ctttttcttg
      241 ctttcatatg taccaatgtt actaattgcg ccattcgtga aagttacctg gcgtccagtt
      301 aagcattatg gtttaaagaa accattataa atcaaacatc aagtgatcaa gatttaattc
      361 acgaacactc ttaacattta ataacactaa aattttcttt aagtcgataa ttaattcatc
      421 aactttttta atcatttcag gaattggtaa gttacataaa tcgagaaaat attttgccat
      481 acctgtcgct ttagcgccca tcgctagtga tttaacgatg gaactagcgt tattaatgcc
      541 gccactagcg agcacttcaa aatttggata attacgagca aggagcaatg catcaacagt
      601 ggaaatacca aaatcaagaa gtggtgaatc ttttttcttt tgtcttttta attcaatcaa
      661 ggagaaatta ataccaccca ttccgcttat atcaatatgc ttaacaccaa gttcttggag
      721 gagtaagaga gtcggttcat gcattccgta tcctacttcc ttaacaatta ccggtacttt
      781 aacggttttt aaaatgttag caatattatc tttccatttt gtaaaatcac gatccccttc
      841 acccatcact aattcttgga cactattaac atgaatcgct aacgcattag cgcccaagat
      901 ttccactgca tcactagcca tttctgcggt gaaattgggg ttaatatttg cgacaataaa
      961 tagatcaggg aagttaaatc taagtgtttc aaatgcaagg cgtgattctt tgtttttaaa
     1021 cgctaaactt tgtgagccaa caaacaaagg aaggccaaaa tgctgggcta atatcgctaa
     1081 tttagcattt atcttatcgc cttttttagc gccgcctgtc attgcattaa tgtaaaaagg
     1141 ataattaaat ttatgtccta aaaatgaagt cgaaagatcg atatctttta atgataaaga
     1201 aggaatacta tgatggataa aacgaatgtg actaaaatca ttttttctta tcttagctat
     1261 ttttgcaatc gcaatatgtt catctttacg gcgtctactc attatctttc ctccaaatat
     1321 caagtacgat gataccttca ttttcccata aatttgttaa tggttcaaca acactctggt
     1381 cattaatttg ggcaatcccg caatcaccat atcctgcccc tgatatttta gcggccactc
     1441 ctaaagagcg ggcagttttc actaatgctt ctaaacgcgg agtaatcatt ccttgtcgcg
     1501 ttaatcctaa tttatcaagt aggttttgat attgttttat ataaatatca tcgtaagtaa
     1561 ctaaataatg gtgaacaagc ttatttgctt ttttaaagaa agcggaatca atagtgccgt
     1621 tagtgtctgg tgttattgtt tcttttgtcc aacccacaag gaaggtagga agttttgtta
     1681 ataatttaat ttctaaaagc ggccacgcaa cttcaagtaa ttcaaatgtt cgtttttcat
     1741 taagtaacca atttaaatca taacgaacat aataaagcgg catatcaaaa atagtacacg
     1801 ctaagtcgcc accagaagta agcgcattta gttcacgctg gattaagact gccattttaa
     1861 aaagcacaag tggtgacaaa ttagtgcgat ataaacgatt taataactta agtgttaaga
     1921 caacactcgc cgcacttgat cctaaaccaa acttaacttg ttcatcacta attaaattat
     1981 tttcaatagt atattcaaat tgctgtaaag gaatattttt taaagcgaga tattcattaa
     2041 aaaatgtaat cgtagtaaca gtatttttta attgatgagg acagccattt tctagtaacg
     2101 tttgataggg ataagcgaca ccatcttcaa taattgttgt cttatgatta gcgacaaatg
     2161 ttccttgtaa aaaaagatta acgggcgcga caattgcacc atgtccgctt tttaaaacat
     2221 tgtattcccc gataacataa agtttacctg gtactttttc atgaacgcgc actagtaata
     2281 ccgcctccaa caccacttac tgaaatttta acttctggta aaagcctaat aacttcatta
     2341 acatattcat caagtgtaaa aagaattaag tttggaccag cgtcaaacgt ataataaaca
     2401 ggaattttat gtttagagcg aatcgcttta actttttgaa ttaattgttg tgtttttggt
     2461 gtatgatatt caagccccgc ttcaagcatt acattacgca taagttcagc agcgcgttcc
     2521 gcaatgcgac caacagcatc aatatcattt tctttaagcg cagtaataag cggttcgaca
     2581 gattcatttg attgcttgac ataatcagcg taagacttag ctttttcaat ggtgatttgc
     2641 attccaactc tacttgatat tttcttaaca ctcggatcaa taatagtaaa aagcattcta
     2701 aattctggcc atttaacttg taacttttca gcataacttg tgtcatgact attaccgcga
     2761 tgccaaataa caaatccgcc gtgaattgaa cgagtcgcgg agcctgaacc aagtctagca
     2821 agtttcgaca tttctttttt acttactttc gcaccgattg cgtcaatata agcagcagta
     2881 agcgcggcaa aaccgctcgc acttgaagca tagcctgctt tagtaaaaac tctattgatt
     2941 gaattaacaa tacaatactc tttacgttta aaatgtttac ggagtgcgtc taaatgatgg
     3001 gctactcttg tataagaaat accgcttaat gctgtaccat caagaataac aatatcttta
     3061 gtggcttttg gatctaattc aacattagtg agagtataaa aattatccgc ggtaacggaa
     3121 atacttgttt gataaggaat attaattaaa gcattaactt tcccccaata tttaagaata
     3181 gcgatattta catttgcttt tttatatttc atcacatgtt cctcaaatca atcatccatg
     3241 atggaaagct ctttaatttt tctttagtgg caagtgcttt ttcttcatca ttaaataggg
     3301 caattaaaca tccgcccatt cccccacccg taattttaac accaagcgca cctgcgtcta
     3361 atagcttagt gcgtaattct tcaagtgtag gcgttgaaat acctagcgct cttaaatgtg
     3421 tgtgagcagc gttaaacatc gttgcaagaa gcgtaatgtc attattttct aatgcccatt
     3481 tagcgtttgc agttaatgaa cctaaagcat caatatgaat gtcgcgttca ggatgagtgc
     3541 ttacatgtgc gacagcactt tttgtcgcac tgtttgtatt tgaataaaac accgctaaat
     3601 agccatcaat attaaatttc agtggcttta tgtgcggtct ttgatatata attggctttt
     3661 cataaataac agtagaagcg tcaattccgc ttgcgttatg atgatatata ttttcagaga
     3721 tttttaataa ttcaaagtaa tcaatatgtg ttaattcaag atgaaaatga ttgttaaaag
     3781 catcaattac cgctttagca acactagcgc ttgagcctaa gccgcttttc tctggtaaac
     3841 ttgaacgaac acttataatg acaggcttaa tctttttata aatttcagcc atatgtgtta
     3901 ttaaagcaac aagtccgcgt aattcatcta cggcattatt aagtgggcca gtgtaatact
     3961 tcgtatcaag aataaactct tcgtgcggag ttaaaacaac ttcgcttcgc attgcataaa
     4021 aaggaatcgc aatagcggga tgaccgtaaa cgacggaatg ctcaccaatt aatataattt
     4081 ttgctaaacc gtatccatcc ataattagtc cctaattaga agagcgcttt gaggattgta
     4141 ataaataacc cacctgcgta aagtgccata aactgtacac cgtaacccgc tcctttttta
     4201 tttagccaac cacttagaag gccaacacta actacaatta ttaaaccata taaaccggct
     4261 tcggcaaaag aaagaagcgc gataattgag aacaaaaaga taagcactgt ttcttgtgaa
     4321 atatacttaa atacaagttc agtcatttta tgagaatatt tcataccaag ccacactgtt
     4381 aagctaagtc caattactaa accaataaca atggcgatta ataaataacc ttttggtacc
     4441 gctaacccag cattaccttc aagaataatt ggaccgccag gactaaaaat aacggagcct
     4501 ggaccaagtg ccgctggtga aatagggaaa gcgaagacga aaagtgaaat cataattcct
     4561 gctaaataag tagcgcttga taaaccgtta acagcagaaa caccttgtaa agcttgttgt
     4621 acttcgtctt tttcatcttt cgtcgcaaat tcaccaagca tcattgtgac accaaccggt
     4681 gacatgaaga agagcaaact accaagcgca ctagcgattc cagttttagc aatgatcttt
     4741 ttatgaccat gctttgtttt ctttacatca tcatcaagaa taacagtcat ctttccttca
     4801 accttttggt ttttaaatac actagggaag aaaagttcaa ataacgcaaa cacaagtgga
     4861 ccagtcgtaa tacttaaaaa gaaaggtgtc attgataaag tccggccacc gataaacgta
     4921 tttaagagcg tattattaat aataagacct ggattacgta agaaagcagt ggcaaatcca
     4981 aacggaacga ttgataaaag tgaaacaact ttttccttac ttgttaaact catcaaaata
     5041 gcgccaccaa ggaagatgta ttcattgtaa cgcttaataa agttacttat ttctaatcca
     5101 gtttgtacag aaataaattc aaaaataccc caaaacgcta gggcaatggg gaaactaact
     5161 aagacaccga ttaaagaacc aagcatcatc cactgtacac tataagaagt aagtccgtgt
     5221 ttctttagtg tttgactatg accaacaagc ggtgttgaca ttaccccgcc tgggatcgac
     5281 gttattgctg tcggtatcga gtcagcaata ttaagagtaa caactgcagc aataaagcca
     5341 gccataatca caagtgggtt aagttttaaa gtaactagaa taagtgtcat tggcactaag
     5401 actgaagttt catcagtgcc agggataata ccgataatca gatataaagc ggtcataagg
     5461 agagcaatcc cgaccatttg taaaattaaa gaaaaagtaa taaccataaa ttagccctct
     5521 ctttctttct tgtttaaatc acttgattta acaatattgc gttggggctt aatccaaaaa
     5581 ttattaatga caaaagcaac aagaatcgct aatgtcccaa acccataaat aaaatctttc
     5641 tttttttcgg caatgatcgc aacatgtttt gctaatactt caatagtaac aaatgttaat
     5701 gccacgaagg ctaggctaat agcaagccct ataaacagtg atgatattac aatgtgatct
     5761 ccccacaagc gcaccatcgt tttcttcttt ttcataacat caaacctcgc ttgaaagatc
     5821 attatagcat tttaagtaat taatcccaaa acacataagc aaaaatcaat tattcagtaa
     5881 gaaataattg aaaagtaata atgataataa taaaaaagat gaccccacta gggtcatctt
     5941 tcgatagtgt atttagcgaa ttattcgtct ttttgtgctt tttcttttaa cactactggc
     6001 gcaggttcac cgtgcttaac aaagtacatt ggaatgaacg ctaggcccat aaagaccgca
     6061 ctataaataa agagtactgg gtaccaaccg gtaatatcca ttaatgcccc agaaagaatc
     6121 ggtgtaataa tcattgcact catactagcg ctatagtagt aaccagtgta aacaccgata
     6181 ttaccgtgac gcgacatttc cacaaccatc ggataactat taacattaat tgaagcccaa
     6241 ctaatgccga caagacccat gataatgtac attaatactt tagtttcatc agtaataaag
     6301 acggcactac ctaaagcaat tgttaacaca acaataccga agagaatcat acggcgacga
     6361 ccaatttttt gtgaaatgta accaattgga ataaacgcta acgcagcggt aataatgcca
     6421 ataagtggtg gtaaactaaa ttgttcaagt cctaaatggt taaaagcata aacagaaaac
     6481 tttgtagtcg cagcgttata acccataaac cataatacaa cagcgcttaa gatgagaata
     6541 aaactacgac gtacatcttt tggcattttt tcgtcatcac tatgttgtga tacatctaaa
     6601 ccttcgtcat caatatctaa tgaatcaagg tgatactttt cagtgatttc gaccatttct
     6661 ttgtaccatg cgttttcttt aacagtcgca acaaagatag caattaataa aagcattagt
     6721 aaactagttg ttgcaaaagt agcgacatag cccgttttat caaagatagg accgcttgca
     6781 agtaagaaca ctaagacata ggcaaacgca ccacccatgg tccccataaa gttaatgaca
     6841 gcattagctt tactacggag tggtttaggt gtaacgtctg gcattaagct aacagcagga
     6901 ctgcggtaaa ttcccattgc cactaagacg aagaataaaa caacaataaa gccaataagt
     6961 aaaccaggtt gttgttttgt tacttg
//
LOCUS       DAOSEX010000008         6559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_10251_length_6559_cov_3.381919, whole genome shotgun sequence.
ACCESSION   DAOSEX010000008 DAOSEX010000000
VERSION     DAOSEX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10251_length_6559_cov_3.381919"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..2468
                     /locus_tag="PK612_00395"
     CDS             165..2468
                     /locus_tag="PK612_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94615.1"
                     /translation="MAETVKGMNIKLGLDTTELEANIKSLNAGLKEQQRDLAVINKNL
                     RYDAGNLDLWKQKQDKLNQTLTLTKQKLEEQNKQLEKAKKAVEIGAMSEKEFNKLNRS
                     IKYTEAEVANLNSQLEKTESKIKSLSSINTAAIGKIGSALTKYVTAPAIAAGSALSAL
                     ALSTAKTVDDIADTAKKLGLSVEALQEWQYTAKLLGSTTIAMDKAFIKVNTILGNIAS
                     GNADRAREALALIGLTVDDIAGLNTEEAFEVIRNALASVEDQATRTAAANAFFGENIG
                     SELAPVLEASATEISKWKEEARELGIVTSEDAEIAGSFNDALDSLALATRSLAMSFAT
                     LLLPVLEKIVVALRDKLIPSVQKVLDWWKSLNSSVKVIIGVVAGFLIALGPSLVVFSK
                     LVPILTSVKTAFTAVSGAVKVAGLAVKFSTLGWAALIAVIAVVLLQNEKFRALLKRLI
                     DIVGDLLKRVAGFASTLVQSLMPVIVAVMSVINTLIDVVVSLLDDVLDLVIDIIDVII
                     NLIEALIPVITKIINMVVGLLIPIINAVLVLLNPIVKIIKILIGLIVKIAQVAFTLIN
                     SVIGPLIKVITVVADILNIIIKLVVSLLDIAVQILNPILQIIVALLIPIIEVISVIIE
                     VISVLMVLLEPLINVLLAPLLVMLEVIFTIIEALTPVLLVLSNVIKAVIVPVLEILFV
                     ILEPILAVLTEIIDGVKWLLDKASGVFNWFAELFNGEAFTDTNAVKNNSVTNNKVENK
                     TTTNNVTINTTGDVDIDSINAALGGAY"
     gene            2468..3292
                     /locus_tag="PK612_00400"
     CDS             2468..3292
                     /locus_tag="PK612_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94616.1"
                     /translation="MRKLYLVNEVGTTYYLDYRNNTLIESLDGFGFDFNIEYQDFADK
                     FVENKRKVQQRIINVTLLFLEGYEGFTIWREFITNTKKMRLYYYADGLKYCYVNVKST
                     SKSQLESFIIRSSVSIECLSLWLVDKTEVINVVESNEGKIYPYNYAYIYSVSYNGSVT
                     LNNECVKSVPLKIKITGNLLNPRVIIRQNGIEVSKLRLLLDERDEPVIEVSSDPTNQY
                     IKRIVDGVETDIYEYQDFSYDNFLFLPPGISEVFFDPGVMEPSTCEISFSEEYIAH"
     gene            3304..4365
                     /locus_tag="PK612_00405"
     CDS             3304..4365
                     /locus_tag="PK612_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94617.1"
                     /translation="MQIIFLSERTLAVLGYGYASDDFEIILDSLVPQQSFFHVNLNSI
                     SASVGDYLTVRKKNYFYIGIITAIEKIEDNYIKVTTKDFFSKFDVEVPVNSYTGNISQ
                     FIVNLIRAHFVYSSDPKQNLSYLQTQIMINKTGTLNYEPDKKVNILKLVAEFSKTYGL
                     RLGYELVITNGQITNIKILVLAITKGLIIKSNLGAITNLNIADSNTNALNKIIFYPKA
                     DNVTYQNIITYYLLNDGTISNYTNSPKRIPIVSFKCEFYSDKDYTSLSTKATSALIDS
                     SLEHNITFDFSFLTNRITDLTDLSVGTFVNFITPHKTYETLVTKMVYKGTFNKATITL
                     GEQRISLTDKLKLLNRRGI"
     gene            4368..5147
                     /locus_tag="PK612_00410"
     CDS             4368..5147
                     /locus_tag="PK612_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94618.1"
                     /translation="MALVKITFDSASVSAKQDADCNHFLASNQNGRIIGLGGNVAVTT
                     SNNYIILSSGYVQVYGRRVYVEANTKIAVALTGSAYGYVFIKFDLGNNEVSLEKKEAP
                     SSYPSLIQENLQFGGLIYELPVARYTKTASSLNLDYYYDPPEIVNADTLAEARVVALD
                     LAVKNRYGPVWQMNYSSVSGKFFTYDNIKTSNASNGFGNVNVAGWNVVFSTAAASGSG
                     AIYQYKFNGTWYDVAIQLKDTGLVVEPALASHAPGRVYVAR"
     gene            5134..5736
                     /locus_tag="PK612_00415"
     CDS             5134..5736
                     /locus_tag="PK612_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94619.1"
                     /translation="MLPVSIYGKKVLAIYRCGSVLHGVEKDDSDRDFIVILKNYNDVR
                     LCKVDNADYFLFGVNKFKQAVNFDRRIADYYLIWLDNTLIAKENTLFVDESFKEEYYA
                     LINIDWKKYFKVWLRINYEYFSACFDGHINEKTLYNLYRLRSLLVHYENTRKFEYYLS
                     AEDKALIIDYKNKKENLEKHYANFKTIFAFIKKVLDKEER"
     gene            5736..6056
                     /locus_tag="PK612_00420"
     CDS             5736..6056
                     /locus_tag="PK612_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94620.1"
                     /translation="MEEISNVLAVISVLGTLSSIFFAVLAFRRNDRGDQKQIGKKEGE
                     VISDIRHIKSSVDRVEKTLDKLEERYNDLDGRLIKVETDTANLAEKIDHRVRTKARYV
                     KGGS"
     gene            6060..6440
                     /locus_tag="PK612_00425"
     CDS             6060..6440
                     /locus_tag="PK612_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94621.1"
                     /translation="MDQILINIISVIVTAVVIPIISLLGTKLIQWIGTKTNNEKATKI
                     LSDATTIVLNSVKTVFQTYVDTLKKSGSFNKDAQDIALSKAKDIALSQLSTETIKFIE
                     NSYGDINNWLTIQIEATISTLKNH"
BASE COUNT         2155 a         1115 c         1265 g         2024 t
ORIGIN      
        1 tggcgcaatt aggaattcca atccgtgata gtgagttttt tgacattcaa acttacctcg
       61 aaatcgtcga aatccacaag aatgtctatg caggtgagac acctagtcga caagcaacac
      121 aagcggatat cgatacattt ttaggttagg gggagtataa aaatatggct gaaacagtaa
      181 aaggaatgaa tattaagtta ggtcttgaca ctactgaact tgaagccaat attaaatcac
      241 taaatgctgg ccttaaagaa cagcaaagag acctagcagt tattaataaa aacttaaggt
      301 atgatgctgg gaatcttgat ttatggaaac aaaagcaaga taaactaaat cagactctaa
      361 ccttaacgaa acaaaagtta gaagaacaaa ataagcaatt agagaaggct aaaaaggctg
      421 tcgaaatcgg cgccatgagc gagaaagagt ttaataaact taaccgctca ataaagtata
      481 ccgaggctga agtcgcaaac ttaaattcgc aacttgaaaa aacggaaagc aagattaaaa
      541 gtctctctag tatcaacact gctgctatcg gtaaaatcgg ttcagcatta actaaatacg
      601 ttactgctcc cgctattgcg gctggctcgg ctttgtccgc tctagcactt agcacagcaa
      661 agacagttga tgatattgcc gatactgcca aaaaactagg actttcagtt gaagcattac
      721 aagagtggca atacacagct aaactattag gctcaaccac gattgcaatg gataaagcct
      781 ttattaaggt taatacaatc ctcggtaata ttgcttcggg gaatgcggat agagcaagag
      841 aagcactcgc ccttatcggg ttaacggttg atgatattgc tgggcttaat acggaagagg
      901 cttttgaagt tattcgtaat gcactagcaa gcgttgagga tcaagcaacg aggacggcag
      961 ccgcgaatgc tttctttggc gaaaatattg gttcagagtt agcgccagta ttagaagcgt
     1021 ccgctactga aattagtaag tggaaagaag aagcaagaga gttaggaatt gttacgagtg
     1081 aagacgctga gattgctggc tcttttaatg atgcgctcga ttcactagcg ttagccacta
     1141 ggtcgctagc gatgagtttt gcaacactct tactacccgt cctagaaaag atagtggtag
     1201 cattacgaga taaacttatc ccgagtgtac aaaaagtact tgattggtgg aaaagtttaa
     1261 atagtagcgt taaagttata attggagtag tcgcgggatt tctaattgct cttggtcctt
     1321 cacttgttgt attttcaaaa cttgtcccaa tattgacgag tgttaaaacg gcatttacag
     1381 cagtgagtgg tgcggttaag gtggcaggac tagcggttaa attttcaaca cttggatggg
     1441 cggcgcttat tgcagtaatc gctgtagtat tactacaaaa tgaaaagttc cgtgctttat
     1501 taaaacggct aattgacatc gttggtgatt tacttaaaag ggtggctggg tttgcctcga
     1561 ctttagttca gtcactcatg cccgttatcg tagctgttat gagtgttatt aacaccttaa
     1621 ttgatgtagt cgtttcttta cttgatgacg ttcttgattt agttatagat attatcgacg
     1681 taatcattaa tttaattgaa gcactaattc ctgttattac gaaaatcatc aatatggttg
     1741 tagggctttt aataccaatc attaacgcag tactcgtctt actaaatcca atcgtcaaaa
     1801 taatcaaaat actaattggc ttaattgtta aaatcgctca agtagcgttt acactcatta
     1861 attcggttat tggtccgctt attaaagtga ttacagtagt agcagatatt cttaatatca
     1921 ttattaaact cgttgtttca ttacttgata ttgcagtcca aatcttaaac cctattttac
     1981 aaataatcgt agcgctcctt atcccgatta ttgaagtcat cagtgtcatc atcgaggtta
     2041 ttagcgtttt aatggtttta cttgagccac ttataaatgt gctacttgca ccacttttag
     2101 tcatgctaga agtaattttt acgataatag aggcactgac gccagtttta cttgtccttt
     2161 ctaatgtaat aaaggctgtc atcgttccgg tacttgagat actttttgtt atccttgagc
     2221 cgattctagc ggtgctaacg gagattattg atggtgttaa atggctactt gataaggcta
     2281 gcggagtgtt taactggttt gctgaactct ttaatggcga agcattcacg gatacaaatg
     2341 cggtcaagaa taatagtgtc actaataata aagtagaaaa caagactacg acaaataacg
     2401 tcacaattaa tacaacaggc gacgtcgata tcgattcgat taatgccgct ttaggaggtg
     2461 cttactaatg cgcaaactat atttagttaa cgaagtaggc accacctact atttggatta
     2521 tcgaaacaat acactgattg aatcgcttga tggttttgga tttgatttta atatcgaata
     2581 ccaggatttt gccgacaaat tcgtcgaaaa caagagaaaa gtccagcaaa gaataattaa
     2641 tgttacactc ctttttcttg aaggatatga aggctttacg atttggcgag aatttatcac
     2701 taacactaaa aaaatgcgcc tttactatta tgccgatggt cttaaatatt gctacgtcaa
     2761 tgttaaatca acatcgaaaa gtcagctcga atctttcatc attagaagca gtgtcagtat
     2821 cgaatgctta tcgctatggc tagttgataa aacggaagtg attaatgttg tcgaatctaa
     2881 tgaagggaaa atttatccct ataattacgc ttatatctac tcagtatctt ataacggttc
     2941 cgtaacgctt aataatgaat gcgttaaaag tgttccactt aaaatcaaaa ttacgggaaa
     3001 cttattaaat ccccgagtaa taattcgcca aaatggaatt gaagtaagta aacttcgctt
     3061 gctacttgac gaaagagatg aaccagtaat tgaagtatct agtgacccta ctaatcaata
     3121 tataaaaaga attgtggacg gagtagaaac tgacatatat gaatatcaag atttctccta
     3181 tgacaatttt ctttttctcc cacctggtat tagcgaagtt ttctttgatc caggagtgat
     3241 ggaacctagt acttgtgaga ttagttttag cgaagagtat atcgctcact aagaggagtc
     3301 tgtatgcaaa ttatcttttt aagtgaaagg acacttgcgg tattagggta tggctacgct
     3361 agtgatgatt tcgagattat tcttgattca ttagttccgc aacaatcttt cttccatgta
     3421 aatttaaaca gtattagtgc gagtgtcggt gattacctta ctgttcgcaa gaagaactat
     3481 ttctatattg gtatcattac ggctatcgaa aagattgagg acaactatat aaaagttact
     3541 acgaaagatt tcttctctaa atttgatgtt gaagtgccag ttaattccta caccggtaac
     3601 atctcgcaat ttattgttaa tttaattcga gcacacttcg tttatagtag tgaccccaag
     3661 caaaatctta gctacctaca aacgcaaatc atgattaata agacgggtac acttaattat
     3721 gagcccgata aaaaagtaaa tattctaaaa cttgttgccg agttttcaaa aacttatgga
     3781 ttgcgacttg gctatgaatt agttattaca aatgggcaaa tcacgaatat taaaatatta
     3841 gtcttagcaa ttacaaaagg tttgattatt aagagtaatc taggcgctat cactaactta
     3901 aatattgcgg actctaacac taacgcacta aataaaatta tcttttatcc aaaagctgac
     3961 aacgtcacct atcaaaacat tattacttac tatcttttaa atgacggtac gattagtaac
     4021 tacacaaact cgcctaaaag aataccgata gttagtttta aatgtgagtt ttatagtgat
     4081 aaagattata cctcgctatc aacgaaggca acttccgcgc ttattgattc ttcactagag
     4141 cataatataa cttttgattt ctcattttta acgaatcgaa ttaccgattt aactgactta
     4201 agtgttggta cttttgttaa cttcatcact ccgcataaga cttatgaaac gctcgtaaca
     4261 aagatggtgt ataaggggac atttaataaa gcgacaatca cgcttggtga acaacgcatc
     4321 tctttaacag ataaactcaa attactaaat agaaggggga tttaaaaatg gccttagtaa
     4381 aaattacctt tgactcagct tcagtgtcag caaagcaaga cgctgattgt aatcattttc
     4441 ttgcctcaaa tcaaaacggt cgaataatcg gcttaggtgg gaatgttgct gttacaacga
     4501 gtaataacta catcattctt tcaagtgggt atgtccaagt ttatggacga agagtgtatg
     4561 tagaagcaaa cacaaaaatt gctgtggcac ttactggtag tgcttatggc tacgtattta
     4621 ttaaatttga cctcgggaat aatgaggtct cacttgaaaa gaaagaagcg ccaagttcct
     4681 atccttcgct tattcaagaa aatttacaat ttggtggact gatttatgaa ttaccagttg
     4741 ctcgttacac aaaaacagcg tcctcgttaa atctagacta ttactatgat ccacccgaaa
     4801 ttgttaatgc tgacacactg gcagaagccc gtgttgttgc gctcgactta gcagtcaaaa
     4861 atagatatgg tcctgtatgg caaatgaatt atagttcggt aagcggaaag ttctttactt
     4921 atgacaatat taaaacaagt aatgctagta atggtttcgg taatgttaat gtcgcagggt
     4981 ggaatgttgt tttttcaaca gcggccgctt cgggcagtgg ggcaatctat caatataagt
     5041 tcaatggcac ttggtatgat gtcgcaattc aattaaaaga tactgggctt gttgtggaac
     5101 cagccttagc atcgcacgct ccaggaagag tttatgttgc ccgttagtat ctatggtaaa
     5161 aaggtactcg ccatttatcg gtgtgggtca gttttacatg gtgttgaaaa agatgacagt
     5221 gaccgtgact ttatcgtcat tcttaaaaac tataatgatg taagactttg taaagtagat
     5281 aacgccgact atttcctttt tggtgtaaat aaatttaagc aagcagttaa tttcgataga
     5341 cgcattgccg attactattt aatctggctt gataatactt taattgcgaa agaaaacaca
     5401 ttattcgtcg atgagagttt taaagaagag tactacgctt taattaatat tgattggaag
     5461 aagtacttta aggtgtggct acgcattaac tacgaatact tttcagcgtg ttttgatgga
     5521 cacatcaatg aaaaaacact ctataacctt tatcgtttgc gctccttatt agttcattat
     5581 gagaacacaa ggaaatttga atattactta agtgctgaag ataaagcgct aattattgat
     5641 tataaaaata aaaaggaaaa tttagaaaaa cactatgcca actttaaaac aattttcgcc
     5701 tttattaaaa aagtgctcga taaggaggaa cgctaatgga agaaatatct aatgttctag
     5761 cagtcatcag tgtacttggt acgctttcat ctatcttttt tgcagtttta gcttttaggc
     5821 gtaatgatcg tggtgaccag aagcaaatcg ggaaaaaaga aggagaagtc atctctgata
     5881 ttcgccacat taagtcttca gttgaccgtg tcgaaaaaac gctcgacaaa ctcgaggagc
     5941 ggtataacga tttagatggg cgtcttatta aagtcgaaac tgataccgct aatttagccg
     6001 aaaaaattga tcatcgtgta cgcaccaaag cacgatacgt aaaaggaggt tcttaattta
     6061 tggatcaaat tctaattaat atcatttcag tcattgttac ggcagtagtt attccgataa
     6121 ttagtctgct cggcacaaaa ctcattcagt ggattgggac taaaacgaac aatgaaaaag
     6181 caacgaaaat attaagtgat gcaacaacta ttgtcttaaa ttcagtaaaa acggtgtttc
     6241 agacatatgt tgatacttta aagaaaagtg ggtcatttaa taaagatgcg caagatatcg
     6301 ctttaagcaa ggcaaaagat attgcattat ctcaattatc tactgaaacc attaaattta
     6361 tcgaaaatag ttatggcgat ataaataatt ggcttactat tcaaattgaa gcaacgatta
     6421 gcacattaaa aaatcactaa actataattt atcatctcgg ctcctattga tttaggggcc
     6481 tttttttatt attggtgtta aatttttcat aattccttcc tatttatata gtgagcattc
     6541 atgactgctt cctatataa
//
LOCUS       DAOSEX010000009         6432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_10551_length_6432_cov_3.560608, whole genome shotgun sequence.
ACCESSION   DAOSEX010000009 DAOSEX010000000
VERSION     DAOSEX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10551_length_6432_cov_3.560608"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="PK612_00430"
     CDS             <1..1283
                     /locus_tag="PK612_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94622.1"
                     /translation="NMKNNKTKLFLLTALLMLVVSSCGESSLSSDNSISSISVSETTT
                     NVVSTSNEDSLAETTTEDSSEPHNESSVEPSTSESSAEIPSSSTAPLVETLVRTIDFE
                     ELDAGNMSATHHNTYYDGLIVADGRVTTTKKTSIDNKSLGMRMPASTTEEPRICTIKV
                     SGYTKIVLDLKQERTHTRIKTNTGAIYQGTKNEDILRDVEILTTELTELVFTFYTETL
                     ISSTMDCGFDNIRFYALVEEGGLPPSSETPTSSEPISSSEETSTTSEIPTSVDGTYDY
                     ITMRDIAEFLQTKDAHYPISDAFIKEYQQKTGGYIDYARQLGKVVDKSICEPDQKWHF
                     FDSWLYRTLEEGGITWTDSAKNRVYTKLLCPELLLWIYEACAAPEAKILAAYDAAVAG
                     KEAGTHISTIAKNMRACVPWEDLEINIQYFLDNK"
     gene            complement(1495..5022)
                     /gene="nifJ"
                     /locus_tag="PK612_00435"
     CDS             complement(1495..5022)
                     /gene="nifJ"
                     /locus_tag="PK612_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOH94623.1"
                     /translation="MKKKFVSLDGNAAAAEVSYLYTDIAAIYPITPSSPMAELVDLFA
                     SQGKLNYFGSKVKVTELQSEAGAAGAVHGALQAGTLATTYTASQGLLLMVPNIYKWVG
                     ELLPAVLHVSARSIAARSLSIFGDHQDIYAVRQTGVTMIASHSVQEVADLAHAAHLTA
                     IGSSAPVLHFFDGFRTSHEIQKVEVIDENEWKKLLPEDKVAEFRARALNPHANPVTRG
                     GAENDDIYFQGKEAQNTHYARVLDVACDYFAKVSKVTGRHYAPFVYLGAKDATKVIVA
                     MGSVTETIREVVADLNKKGEKVGLVKVHLYRPFSAKHLVDVLPETVKKIAVLDRTKEV
                     GALGEPLYLDVLTALKQQGRKVDVIVGGRYGISSRDTQPKHIKGVFDFLDNKPWHSFT
                     VGINDDVTKLSVPFDHDYHIDNGHTECLFYGLGSDGTVSANKSSVAIIGEHTDKYAQA
                     YFAYDSRKAGGPTRSHLRFGTNTIHSTYYVENASFISASQDSYLYKFDMIRYLKAGGT
                     FLLNTSLKGEELINALPNRVKYGLAMKNAKMYVIDANKIASEIGMGRHTNTILQSSFF
                     YLNQDIMPYEKALRLMKKYAEAAYGRRGKRIVELNYAAIDAGSHGIIEVKVDPEWKKL
                     KPLRTVELMGDKYFDEYVNPIDNLEGYDLPVSTFTKWDLLDGTMTNDITFKQKRSIAT
                     MVPRWVKENCIQCNTCSLVCPHATIRPFLLDEKEVAGLPENVREETVAAMGGPSVAGL
                     RFRIQVSPMNCVGCALCVVTCPGRGGNKALEMVEARSQFDIEPAADYLYKQVTYKTDK
                     FPLNSPKGVGFLMPYMEISGACAGCGETPYYRLITQLFGNDMLVANATGCSSIYCGSV
                     PGTPFTTNKDGEGVAWANSLFEDNAEYGFGMRMANDYKLKLIADIFTAALPKVEPELK
                     ELLEKYLANPRDRKEVRSYLPRLIELLEKSKDKGVKEALKYKDDILDKSVWIIGGDGW
                     AYDIGYGGLDHVLASGANVNVLVLDTEVYSNTGGQMSKATQTGAIAKFAASGKTGAKK
                     NLAMMAMAYGDVYVAQVALGANPVATIRALTEAENYDGPSIVIAYSPCTEHGIKGGLS
                     NHIAVEKKAVASGYWPIFRYDPRLEAAGKSPLTWDMKEPDYTQFREFVMGETRFSQLP
                     TVNPENAEFLLSKSEEEAKKRYFRLKKFE"
     gene            complement(5190..5504)
                     /locus_tag="PK612_00440"
     CDS             complement(5190..5504)
                     /locus_tag="PK612_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH94624.1"
                     /translation="MIVYMNEHFRDPNLTIAKIAESSGYSLSHFSKAFKLYMGHSPIS
                     HLNRLRLSEARYLLHTTHLPFKEIALQCGFKNISTFTESFKQSIGILPKDYRKKYQTP
                     NS"
     gene            5697..6005
                     /locus_tag="PK612_00445"
     CDS             5697..6005
                     /locus_tag="PK612_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94625.1"
                     /translation="MWVGDEEYEDPHGYTIKKSVYDHVMTIKSFTVEQQQKYKYFTNS
                     RKFKIEFRDYTSDKDYSYGTFIYNRKGTLMEIHFDSRFVNEEYSINVSDNLLIYYDFS
                     "
     gene            complement(6081..>6432)
                     /locus_tag="PK612_00450"
     CDS             complement(6081..>6432)
                     /locus_tag="PK612_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOH94626.1"
                     /translation="LHGFNSLVLQNEDGSVGDAYTIASGLDYPGVGPEHAFLHEIGRV
                     KYEAITDEEAAEAFFLLSRLEGIIPAIESSHAVAYAIKYARSVSAGSILVCLSGRGDK
                     DMEFMTRKYPNMYK"
BASE COUNT         1945 a         1299 c         1200 g         1988 t
ORIGIN      
        1 taaatatgaa aaataataaa actaaactat tcttattaac agcactatta atgttggtgg
       61 tatcttcttg tggcgaaagt tcactcagta gtgataatag tatttctagt atttctgtta
      121 gtgagaccac aacaaacgta gtatctacga gtaacgaaga ctctttagcg gaaacaacaa
      181 ctgaagactc ttcagaacca cataatgaat cttcagtgga accatctact tctgaatctt
      241 cagcagaaat accttcttca tccaccgctc cacttgtgga aacactagtg cgcacgattg
      301 attttgaaga attagatgct ggtaatatga gtgccacaca tcataatact tattacgacg
      361 gtttaattgt ggctgacggc agggtaacaa cgacgaaaaa aacaagtatc gataataaat
      421 cactagggat gcggatgcct gcttctacca cggaagaacc acgtatttgc acaattaagg
      481 taagcggcta cactaaaatt gttttggatt taaaacaaga acgaacccac acccgcatta
      541 aaacaaatac aggcgctatt tatcaaggaa ctaaaaacga agatatctta cgtgatgttg
      601 aaattcttac cacagaactt actgagctag tgtttacttt ctatacggaa acattaatct
      661 caagtacgat ggactgcggg tttgataata taagatttta cgcactagtg gaggaaggtg
      721 gccttccacc tagcagtgaa acacctactt cttctgaacc aataagtagt agtgaagaga
      781 ctagtactac aagcgaaatc ccgactagtg tagacggaac ttatgactat attacaatgc
      841 gtgatattgc tgaattttta caaactaaag acgctcatta cccaatttct gacgcgttta
      901 tcaaagaata tcaacaaaaa actggtggtt atatagatta cgcgcgtcaa cttggtaaag
      961 tcgtcgataa atcaatctgt gaacctgatc aaaagtggca cttctttgat tcatggctct
     1021 atcgtacact agaagaaggc ggtataactt ggactgattc agcaaaaaac cgcgtgtaca
     1081 ctaaactatt atgtccagaa ttattacttt ggatttatga agcatgtgct gcccctgaag
     1141 caaaaatact agcagcctat gacgctgctg ttgcgggtaa agaagccgga actcatattt
     1201 ccactattgc taaaaatatg cgcgcgtgtg taccgtggga agacttagaa attaatattc
     1261 aatatttttt agataataaa tagctaatta tttatatttt taaacataga gtacaaagta
     1321 aaaaaacgaa tatttttaat ttctgataat ggtgttatcg ttacacttta taatcgaaat
     1381 tatacagata tcgtatatat tgtcgtatat aatatttaaa aatgaggtcc aaaatgaggt
     1441 ccaaaatgaa aagcctcggg gtcgtcccga ggctctcttt taatttaaag agcgttattc
     1501 aaacttttta agacggaagt agcgtttttt cgcttcttct tcactcttac taagtaagaa
     1561 ttcagcgttt tctgggttca cagttgggag ttgactaaac cgtgtttcac ccatcacaaa
     1621 ttcacggaat tgtgtgtaat ctggttcttt catatcccaa gtaagtggtg atttacccgc
     1681 tgcttcaagc cgtgggtcat aacggaagat tggccagtaa ccactagcga cggctttctt
     1741 ttcaacggcg atatggttac taagtccgcc tttaatcccg tgctcagtac acggactata
     1801 agcgataaca attgatggtc cgtcgtaatt ttccgcttca gtaagcgcac ggatcgtggc
     1861 aactgggtta gcgccaagtg cgacttgggc gacataaacg tccccataag ccatcgccat
     1921 cattgctaag tttttctttg cccctgtttt accacttgcg gcaaatttag caatagcacc
     1981 agtttgcgtt gctttactca tttgtccgcc ggtgttacta tacacttcag tgtctaacac
     2041 aagcacatta acatttgcgc cactagcaag gacatgatca agaccgccgt aaccaatatc
     2101 atatgcccag ccatctccac caataatcca cactgattta tcaagaatgt catctttata
     2161 tttaagtgct tctttaacgc ctttgtcttt acttttttca agtaattcaa ttaagcgtgg
     2221 taagtagctt ctaacttctt tccggtcacg cggatttgcg agatattttt caaggagttc
     2281 ttttagttct ggttcaactt ttggtaaagc cgcagtgaag atgtcagcga ttaatttaag
     2341 tttataatcg ttagccatcc gcattccaaa accgtattct gcgttatctt cgaacagact
     2401 attcgcccaa gcgacgcctt caccgtcttt gtttgtggta aatggtgtac ctgggacact
     2461 accacaataa atcgagctac agccagtcgc atttgctact agcatgtcat taccgaataa
     2521 ttgcgtaatt aagcggtagt aaggtgtttc accacagccc gcacatgctc cgctaatttc
     2581 catataaggc attaagaaac ctacaccttt tggtgaatta agtgggaatt tgtcggtttt
     2641 ataagtaact tgcttatata agtagtcggc agcaggttca atgtcaaatt gactacgtgc
     2701 ttcaaccatt tcaagtgctt tattcccgcc gcgtcctgga caagtgacaa cacaaagggc
     2761 acagccgaca cagttcattg gactaacttg aatacggaag cgtaagcctg ctacgcttgg
     2821 tccacccatt gccgcgactg tttcttcacg cacattttct ggtaagcctg ccacttcttt
     2881 ttcgtcaagt aagaacggac gaattgtggc gtgcggacac actagactac atgtattaca
     2941 ttggatacag ttttctttaa cccagcgtgg caccattgtc gcgattgagc gtttttgttt
     3001 gaaggtgatg tcattagtca tcgtgccatc aagtaagtcc cactttgtga atgttgaaac
     3061 tggtaagtcg taaccttcta agttatcaat tgggttaaca tattcatcaa aatacttatc
     3121 acccataagt tccacagtgc gtagtggttt aagtttcttc cattctggat caactttaac
     3181 ttcaatgata ccgtgtgagc cagcatcgat tgccgcatag ttaagttcga caatgcgttt
     3241 cccgcgacga ccataagccg cttcagcgta ttttttcatt aagcgcaacg ctttttcgta
     3301 aggcataata tcttgattta gatagaagaa tgaagattgt aagattgtgt ttgtatggcg
     3361 acccataccg atttcactag caatcttatt tgcgtcaatt acatacatct tggcattttt
     3421 cattgctaaa ccgtatttaa ctctgttagg taatgcatta attaattctt caccttttaa
     3481 gcttgtgtta agtaagaatg ttccgcctgc ttttaagtag cgaatcatat caaatttata
     3541 aagataagaa tcttgtgaag cactaataaa gctagcattt tctacgtaat aagttgaatg
     3601 aatcgtattt gtgccaaacc gtaagtgact gcgggttgga ccaccagctt tacgtgagtc
     3661 ataggcaaag taagcttgcg catatttatc agtgtgttca ccaataatgg cgacgcttga
     3721 cttattagcg ctcacagtgc cgtcacttcc taaaccgtag aataagcatt ccgtatgtcc
     3781 gttatcaata tgataatcgt gatcaaatgg gacactaagt ttagtgacat catcattaat
     3841 tccaacggta aatgaatgcc acggtttatt atctaagaag tcaaatactc ctttaatatg
     3901 ttttggttgt gtgtcacggc tactaattcc gtagcgacca ccaacgataa catcaacttt
     3961 ccgtccttgt tgtttaagtg cggttaacac atcaagataa agtggttcac caagtgctcc
     4021 tacttcttta gtgcgatcta agacagcgat tttcttcact gtttctggta aaacatcgac
     4081 taaatgtttg gcactaaacg gacgatataa gtgcacttta acaaggccaa ctttttcgcc
     4141 ttttttattt aaatcggcaa cgacttcacg gattgtttca gtaacactgc ccatcgcgac
     4201 gattactttt gtagcgtctt tggctccgag atagacaaac ggtgcatagt gacgaccggt
     4261 tactttacta actttagcaa aataatcaca agcaacatca agcacacgag cgtaatgtgt
     4321 attttgtgct tctttacctt ggaaataaat atcatcattt tctgcaccac cacgggtcac
     4381 aggatttgcg tgtgggttca gtgcacgagc gcggaattcc gctactttat cttctggtaa
     4441 taatttcttc cattcatttt catcaatgac ttcaactttt tgaatttcat gtgatgttct
     4501 aaaaccatca aagaagtgta agactggagc acttgatcca attgctgtta agtgtgctgc
     4561 gtgcgctaaa tcagcgactt cttggacact gtgacttgca atcatcgtca caccagtttg
     4621 ccgtacagca taaatatctt gatgatcacc aaaaatggat aaggaacgcg cggcaatact
     4681 acgggcacta acatgcagta ctgctggtaa aagttcacca acccatttgt aaatatttgg
     4741 caccattaaa agtaaacctt gtgacgcggt atatgttgtc gctaaggttc cggcttgtaa
     4801 agcaccgtgc accgcaccag cagcgccagc ttcactttga agttctgtta cttttacttt
     4861 gctgccaaag tagtttaatt tcccttgtga tgcgaataaa tcaacgagtt ccgccatcgg
     4921 tgaagatggg gtaatcggat aaatcgccgc gatatccgtg tagagataac ttacctctgc
     4981 ggctgcggcg tttccatcta gggagacaaa ttttttctta atagccatat tttgacctcc
     5041 gtttataatg actctattat tataaataat aaaccccaaa aatgtaagcg ttattataaa
     5101 aacaaaacgc acatataaaa accacctatt tatcacgagt ttatcgctat aaatcaatta
     5161 aatgctaact taattatttt tattttctat taactatttg gtgtttgata ctttttgcga
     5221 taatctttag gcaatattcc tatactttgt ttaaaacttt ccgtaaaggt agaaatattt
     5281 ttaaagccac attggagcgc aatttcttta aatggtaaat gtgttgtatg taataaatag
     5341 cgtgcttctg aaagccgtaa acgattaaga tgcgaaatgg gcgaatgccc catgtatagt
     5401 ttaaatgctt tactaaagtg acttaaagaa taaccagaag actccgctat tttggcaatg
     5461 gttaaattag gatcacggaa atgttcattc atataaacaa tcattttttt aatccgcgtt
     5521 gcgtattgtt ttggtttaga ggatcattag ataatttgat cctgtacatc cctgttgatt
     5581 ttgatcggat ggtcgggatt acagaaaaac ttaaaacgcc ataacattca aaagttacct
     5641 taaccggaaa acataatggc gtagactttg aacaaaaata tacactgact gacggcgtgt
     5701 gggttgggga cgaagaatat gaagatccgc acggttacac cattaaaaaa tccgtctatg
     5761 accatgtaat gactattaaa tcgtttacag ttgagcaaca acaaaaatac aaatatttta
     5821 cgaactcccg taagtttaaa attgaattca gagattatac aagcgataaa gattattcat
     5881 acggaacatt tatttataat cgtaagggta cactaatgga aattcacttc gatagtcggt
     5941 ttgttaatga agaatacagt attaatgtaa gtgataattt attaatttat tacgatttta
     6001 gttaactaaa taattttaaa agattctaaa aagctagggc gacctggctt tttcttatgc
     6061 accaattaag tcacaaatta ttatttatac atatttggat acttacgcgt cataaattcc
     6121 atatctttat caccgcgacc acttaaacac actaaaatac ttccggcact cacgctgcgc
     6181 gcatatttaa tagcataagc tactgcgtga cttgattcaa tcgcgggaat aataccttca
     6241 agacgcgata agagaaagaa agcttctgct gcttcttcat cagttattgc ttcgtacttc
     6301 acgcgtccta tttcatggag gaaagcgtgt tctggaccaa cgcctggata atctaaacca
     6361 ctcgcgatag tgtaagcgtc tcccacactt ccgtcttcat tttgcagcac taatgagtta
     6421 aaaccgtgca aa
//
LOCUS       DAOSEX010000010         6206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_11119_length_6206_cov_3.763128, whole genome shotgun sequence.
ACCESSION   DAOSEX010000010 DAOSEX010000000
VERSION     DAOSEX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11119_length_6206_cov_3.763128"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PK612_00455"
     CDS             <1..279
                     /locus_tag="PK612_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94627.1"
                     /translation="SPTFTSGAGNHLVETNSVGVVKGQVNGVANELTSIARFYIIQNV
                     SVPASKTLTGTLEFTFVDNGTDQSMYFVDWPTTGVSRRSLSAFFYRGI"
     gene            281..979
                     /locus_tag="PK612_00460"
     CDS             281..979
                     /locus_tag="PK612_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94628.1"
                     /translation="MKEKKRNIVRTIWLMVLSLVASASLTYAWHISIYNFAQTHNVPG
                     ELTPEISLAHYNYTTNSWQNSTTAEPLSIYLGKMTNIAQLPANSNTYLKIRINETSDA
                     QTKYNVIVNNIETVISNIDGVHAVTSVDYFAAENAQNVFDFYVYTSTSDNLNPLTVFA
                     NLQTMTPFKITTLPHALLSNSATLDTWIYILLKPDIAKVQNIIRLVPIQLSPYNMVFR
                     MSLNGEVKTLDENA"
     gene            966..4196
                     /locus_tag="PK612_00465"
     CDS             966..4196
                     /locus_tag="PK612_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94629.1"
                     /translation="MKTHKFKNFFSKVWISITVFFAFVLTIGYPVYAYFRANIERQVE
                     PTLEIELLFGHLREDLVTGEWGTDTNPYLIMSTEHLSNLSVLQNGTYSHYVNENSVFQ
                     VSDENGDPIYVGGATANQLFTIQSIGNEDYPFVSKIRGITTTDPNKYVTLPTGELTDT
                     SIIGNVRVIPEENQVDIGLFGCVGPDLEGEVPETDYQGEISNILLYNIQINTSTVGVM
                     PSGHYGYAKTGVTKETNHLGILAGHVQYANLDKISVYYSQTNGVAHVDAFNVSAGDTA
                     KYTTSGGIIGFYKRVIVDGEIENPVTSDGSDQGAGQAAAGLGKGIVYAEDIWKFMEEN
                     TTVGNPAPNNSYDLQDTFGDELYDPNDNSYFHVGVFTFAHSRQSRGKDRIAKIWTDAY
                     TNEWTISTNPTNPYTQTTRDVGPATKYTATRITNNEMSWAYTGWIFGRRYYGTLNSPY
                     SSNADYRYMLVYEDTTVNPTKHYALVRYGSTAIGVEVDPNNLVIPDGEFKYFTYENLS
                     QRTADITYPPYEAGFSYRFSGPITMDFRNTTSSAGTLQYAVYGDIVTGIVDGQTKILE
                     APRPLRVNAQSNAVPTSSFMATASTSNPPYVEGYRISPTSNTGNFNKFYIQRTGSSSS
                     GGSTSNWYMTYSTAEGFSAVSTSSPGTYASSFALYAVRYTNNTSNPSESPVATRNILE
                     EFIPAGTTETIDTEHNVLFYTGTYNSSVPTSRYSYEIKDISSLGWIDNRNKAITKGDT
                     ALLIGDPTSYYYIKNASGLERFFGVNTGIPSPVDGPPINVPEGSIGFTVNAKTPDSTE
                     TTATINVIVATDPSQGVEQLITVSRFGSGTTTVGDRQLLSGFVLPPPPGSTITGTKPV
                     YLKTSASSPTYAVYPNFNRLLIAYSFEVPIETVPITYFLEASRGTACFVYLSSDRVAA
                     NDNNPLHENDIVIPSLPNIDYVFKNTTNMIATVGRTDYVPSMTMPYFGLTTNSNYDAE
                     TNPNVDHYLVTAATGFDFVYNISRIYDPSQDEKYFLYIKVHANVNGSGYAAPVDLNSL
                     KIIQQNMNFNYSENSYLDRNTNEFIHSDVVHFTINGYFLDDWSVLE"
     gene            4292..5467
                     /locus_tag="PK612_00470"
     CDS             4292..5467
                     /locus_tag="PK612_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94630.1"
                     /translation="MEMYGGNFIKLIIPIILASLSLGGFLTPNKSLVEAKQNDATRET
                     VVYHEDFENIVGEIYQDTKFWGNGMSIVENAGNHSLLYTYDGGATQIGGINTHQTGLT
                     GNDLFENGKLYRVEFDALVTSVADNATVQFDFNIEGLWTSFKVGKTTAYKTESSNGTV
                     RNVQYVDGQVSATFTCSAGGHYFEIFVANMASGDSFKIDNFKVVCLDDVYSTDNYGTI
                     AGSSQQDHYYVADGTLEPATAASGQKYLHFYGTNNTVDTWEAMFFNHLPLTYTAGKNY
                     RNVIKFLEAPTTTEMYINMVGNWAGHTTFTINPVTGEVTHDSGLETCDITWSDNTLVI
                     DFYCSTTLPEQQIRFVSKSVAGTVSDFKIAEFTVYDLDLLTAKAFAKDLLNKTANTT"
     gene            5698..>6206
                     /locus_tag="PK612_00475"
     CDS             5698..>6206
                     /locus_tag="PK612_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94631.1"
                     /translation="MSNKGKIKLKANTAYKISFDTKGYRVGGNGAYYFLKLRSGQENI
                     MTIGEWYDRPDAPANNKIFTFTTPENSKDLTIVFGIKNEGGYYVDNISLIELPNGHIV
                     DGEDVGFDNNVRPFTPNKGLGYEEGFEDGVLADSLFSFGFNRWGHFTNKPNEVINGKL
                     SFTSELDEVT"
BASE COUNT         2031 a         1106 c         1213 g         1856 t
ORIGIN      
        1 tcaccaactt tcacaagtgg tgcaggcaat cacttagtgg aaacaaatag tgttggtgtt
       61 gttaaaggac aagttaatgg tgtcgcaaat gaattaacat caatagcgcg cttttatata
      121 attcaaaatg tttctgtgcc tgcgagtaaa accttaacag gtactttaga attcactttt
      181 gtggataatg gcactgacca aagtatgtat tttgttgatt ggccgacaac aggtgtatca
      241 agaagatcgc taagtgcttt cttctatcga ggaatataaa atgaaagaaa agaaacgcaa
      301 tattgttaga acgatttggt taatggtgct gtcacttgtg gcaagcgctt ctttaacata
      361 tgcgtggcat atttccattt ataattttgc acaaacacat aatgtacctg gtgaacttac
      421 gcccgaaatc tcactagcgc actacaatta cacaacgaat agttggcaaa atagtacgac
      481 tgcagaaccg cttagtattt atttagggaa gatgacgaat atcgcacagc ttcctgctaa
      541 tagcaatact tatttaaaaa taagaatcaa tgaaactagt gatgcacaaa ctaaatacaa
      601 tgtgatagta aacaatattg aaacagttat ttccaacatt gatggtgttc atgcagttac
      661 tagtgttgat tatttcgcag cagaaaatgc acaaaatgtc tttgactttt atgtctatac
      721 ttcaacaagt gataatctta atcccttgac tgtatttgcg aacttacaaa caatgacccc
      781 atttaaaatc acaactttgc cacacgcatt acttagcaat agtgcgactt tagatacatg
      841 gatttacatt ttgttaaaac cagatattgc taaggttcaa aatattattc ggcttgttcc
      901 aatccaatta tcaccatata atatggtgtt tagaatgtca ttaaacgggg aggttaaaac
      961 attagatgaa aacgcataaa tttaaaaact tcttcagtaa agtatggatt tcaattaccg
     1021 ttttctttgc ttttgtttta acaattggct atccagtata tgcttatttc cgcgctaata
     1081 ttgaaagaca agtagaacca acgctagaaa ttgaactact ttttggtcat ttacgtgaag
     1141 atctcgtaac tggcgaatgg ggtacagaca ctaacccata tttaattatg agcaccgaac
     1201 acttatcaaa cctttcagtt ttacaaaatg gtacttatag tcattatgtt aatgaaaaca
     1261 gcgtgttcca agtttctgat gaaaacgggg acccaattta tgttggggga gcaaccgcga
     1321 accaattatt tacgattcaa tcaattggca acgaagacta tcctttcgta tccaaaatta
     1381 gagggattac cacaaccgat ccaaataaat atgtgacatt accgactggg gaattaaccg
     1441 acactagtat tattggtaat gttcgtgtta taccagaaga aaatcaagtt gatattgggt
     1501 tatttggttg tgttggtcca gatcttgaag gcgaagttcc tgaaaccgac tatcaagggg
     1561 aaatttctaa tatcctttta tataacatac aaattaacac tagcactgtt ggtgtcatgc
     1621 caagcggcca ctacggttac gctaagacgg gggttactaa agaaacaaac cacttaggaa
     1681 tccttgcggg tcacgttcaa tacgcaaatt tagacaaaat cagtgtttat tattcacaaa
     1741 caaatggcgt tgcccatgtt gacgccttta atgtcagcgc tggtgacacc gctaaatata
     1801 caacttccgg cggaattatt ggtttttata aacgcgttat tgttgacggt gaaattgaaa
     1861 acccagtgac gagtgacggt agtgatcaag gtgctggtca agctgcagcg ggtttaggta
     1921 aggggattgt ttacgctgaa gatatttgga aattcatgga agaaaatacg actgttggca
     1981 atcctgctcc gaataattct tatgatttac aagacacttt tggtgacgaa ctttatgatc
     2041 caaatgataa ttcatatttc cacgttggtg tatttacttt tgcccacagt cgtcagtcac
     2101 gcgggaaaga ccgtatcgct aagatttgga cagatgctta cactaatgag tggacaattt
     2161 ccactaatcc aacaaatccg tatacacaaa caacgcgcga cgtcggtcct gcgacaaaat
     2221 ataccgcaac acgcattact aataatgaaa tgagttgggc ttatactgga tggatttttg
     2281 gtcggcggta ttacggaaca ctaaactcac catatagtag taatgctgat taccgttata
     2341 tgcttgtgta tgaagacacg actgttaatc cgacaaagca ttatgcactt gtgcgttatg
     2401 gttctactgc gattggtgtc gaagttgatc ctaataacct agttatacct gacggtgaat
     2461 ttaaatattt cacttatgaa aacttaagtc aaagaactgc tgatattact tatccgcctt
     2521 atgaagctgg attctcttat cgttttagtg gtccgatcac aatggacttt agaaacacta
     2581 cttcaagtgc agggacttta caatacgccg tttatggcga cattgtaacc ggaatagtgg
     2641 acggacaaac taaaatttta gaagcaccgc gcccattacg ggttaatgcg caaagtaatg
     2701 cagtgccaac atcaagcttc atggcaacag cgtctacgag caacccgcct tatgttgaag
     2761 ggtatcggat tagtcctact tcaaatacgg gtaattttaa taagttttat attcaacgga
     2821 ctggttctag tagtagtggt ggaagtactt caaactggta tatgacttat tcaactgccg
     2881 aaggctttag cgctgttagt acttcaagcc caggaactta cgcgtcttcc tttgcacttt
     2941 acgctgtgcg ttacacgaat aacacgagta atccaagtga aagcccagtg gcaacccgta
     3001 atattttaga agaatttatt ccagcaggaa caacagagac gatagatact gaacataatg
     3061 tgctctttta caccggtact tataattcaa gtgtaccgac ctcacgctat agttatgaaa
     3121 ttaaagatat tagttctctc gggtggattg ataaccggaa taaagcaatt acaaaagggg
     3181 acactgcact attaatcggt gatcctactt cttattacta tattaaaaac gcttctggcc
     3241 ttgagcgttt cttcggtgtt aatactggga ttccttcgcc ggtcgacggg ccgcctatta
     3301 atgtgccaga aggtagtatt gggtttacag ttaacgctaa aacgccagat tcaacagaaa
     3361 caacagcaac aattaatgtt attgtcgcta ctgacccaag tcaaggcgtt gaacaactta
     3421 ttactgtaag ccgttttggt agtggaacca caactgttgg tgaccggcaa ttattaagcg
     3481 gctttgtatt accgccaccg ccaggttcaa caattactgg cactaaacca gtgtatttaa
     3541 aaacgagtgc atcaagcccg acgtatgctg tttatccaaa ctttaacagg ctcttaattg
     3601 cctactcttt cgaagtacca attgaaacag taccaattac atatttcctt gaagcatcgc
     3661 gcggaacagc atgtttcgtt tacttatcaa gtgaccgcgt cgcagccaat gataataacc
     3721 cgttacacga aaacgatatt gtcatcccgt ctttaccaaa tattgactat gtatttaaaa
     3781 atacgacaaa catgattgcg acagtcggta gaacagatta cgtaccatca atgacaatgc
     3841 cgtattttgg attaacaacc aattctaatt acgatgctga aactaatcca aatgtcgatc
     3901 attatcttgt cacagcggca acagggtttg attttgtcta taacatttca cgaatttatg
     3961 atccaagcca agatgaaaag tatttcctat atattaaagt acatgcgaat gttaacggta
     4021 gcgggtatgc tgcaccagtt gatttaaata gtttaaagat tattcaacaa aacatgaact
     4081 ttaactatag tgaaaactct tacctagatc gtaacacgaa tgaatttatt catagtgatg
     4141 ttgtgcattt caccatcaac gggtatttcc ttgatgattg gtcggtttta gaataaataa
     4201 aaataagaag taataactta ttaacaaaac aacaaaacta catcaatacg caacaacaat
     4261 gttttttttt acgcttttca tgtatgttag aatggagatg tatggaggta attttataaa
     4321 gcttataatt ccaataattt tagcgtccct ttcgcttggc ggatttttaa cgcccaacaa
     4381 aagcctagtg gaagctaaac aaaatgatgc gacacgcgaa acagtcgttt atcatgaaga
     4441 ctttgaaaat attgttggtg aaatttatca agacaccaaa ttttggggca acgggatgag
     4501 cattgtcgaa aatgctggca atcattcact tttatacact tatgatggtg gagctacaca
     4561 aataggtggt attaacacac atcaaacagg tcttactggt aatgatttat ttgaaaacgg
     4621 taaattatat cgtgtcgaat ttgatgcatt agtaacaagc gttgcggata atgcgacagt
     4681 gcagtttgac tttaatattg aaggattatg gacaagtttt aaagtcggca aaactactgc
     4741 atacaagacc gagagttcaa atggtacagt ccgaaatgtt caatatgttg atggacaagt
     4801 ttcagcaacc tttacatgtt cggcaggtgg acactatttt gagatatttg ttgctaatat
     4861 ggcaagcggc gattctttca aaattgataa tttcaaagta gtgtgccttg atgatgtcta
     4921 ttcaacagat aactatggga caattgcagg ttcatcacaa caagatcatt actatgttgc
     4981 ggatggcact ttagaacctg ctactgcagc aagtggacaa aaatatttac atttctatgg
     5041 tacaaacaac actgttgata catgggaagc aatgttcttt aatcacttac cactaacata
     5101 tacggcaggt aaaaactatc ggaatgttat taaattttta gaagctccaa ccacaaccga
     5161 aatgtatatt aatatggttg ggaactgggc cggacataca acatttacga ttaatccagt
     5221 aacgggtgaa gttacccatg atagcggact agaaacttgt gatattacgt ggagtgataa
     5281 cacattagtg attgatttct actgttctac gacattacct gaacaacaaa ttcgatttgt
     5341 ttcaaaaagc gtcgcgggta cagtatctga tttcaagatt gctgaattta cggtttacga
     5401 tttagattta ttaaccgcca aggcgtttgc gaaagattta ttaaataaga cggcaaatac
     5461 cacttagaaa tagcgggatc aggtatttat caaaataaaa taagttataa aaatattaag
     5521 ataaacaagg tcagcagcag tctcattaat tttgaaggga aatataatat tgaaactgac
     5581 tcgtggtata cagacagtat ttatactgat atttatcgcg atgaagaaaa agacattaat
     5641 ggttcaaatg ttgtcttaat cgaaagtaat gttttgcatg gtgagcaaca agggcttatg
     5701 agtaataaag gaaaaatcaa attaaaagcg aacaccgctt ataaaataag ctttgacact
     5761 aaaggatatc gcgttggtgg caacggtgct tattatttcc ttaaattacg ctctggacaa
     5821 gaaaatatca tgacaattgg ggaatggtat gataggcccg atgcgcccgc gaataacaaa
     5881 atatttacat ttaccacgcc agaaaacagt aaggatttaa caattgtctt tggtattaaa
     5941 aatgagggcg gatattacgt tgataacatt agccttatcg aattaccaaa tggtcatatt
     6001 gttgatggcg aagatgtggg ctttgataat aatgttcgac catttacacc aaataaaggt
     6061 cttggctatg aagaagggtt tgaagatggt gttttagcag atagcttatt ctcatttggt
     6121 tttaatagat ggggtcactt tacaaataag cctaatgagg taattaatgg aaaattgtca
     6181 tttacaagtg aattagacga ggttac
//
LOCUS       DAOSEX010000011         6163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_11212_length_6163_cov_2.880485, whole genome shotgun sequence.
ACCESSION   DAOSEX010000011 DAOSEX010000000
VERSION     DAOSEX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11212_length_6163_cov_2.880485"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..588
                     /locus_tag="PK612_00480"
     CDS             166..588
                     /locus_tag="PK612_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOH94632.1"
                     /translation="MQDLLFVKSIDRLGRDYDMIIEEWHHLTKVLEADIVVLDMPLLD
                     TRNDATNLVGKFISDIVLQILSFVAETEREFIKQRQAEGIRLAKERGVHMGRPPLVLP
                     DNFADVVLLFDKEKITCTKAAALTNMSRASFLKYASSF"
     gene            complement(928..1001)
                     /locus_tag="PK612_00485"
     tRNA            complement(928..1001)
                     /locus_tag="PK612_00485"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(967..969),aa:Cys,seq:gca)
     gene            complement(1101..1373)
                     /locus_tag="PK612_00490"
     CDS             complement(1101..1373)
                     /locus_tag="PK612_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOH94633.1"
                     /translation="MSMTNLNIRMDKKVKEEAEAIFQELGLNMTTAVNIFLRTTIREK
                     GIPFHLSLSVSNVTTKAAIEEGRKIALTDNVKGYDTVSDLKAALED"
     gene            1593..2369
                     /locus_tag="PK612_00495"
     CDS             1593..2369
                     /locus_tag="PK612_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94634.1"
                     /translation="MQPILEVKNLVKTFKLSKKQQKIEKTNTKVRVAVNDLSFNVYEG
                     EIYGLLGPNGAGKTTTLRIISTLIKKDSGEVKVNGYDISKEQSKVRSQIGFLTSELKL
                     DDFFTPNYLFDYFSHLHGVPEDVIEKRKQELFDKFGISEFKEVKVSDLSSGMKQKVSL
                     VISVVHDPKFIIFDEPTNGLDVITAKTVIDFLVALKEAGKTVILSTHIFSLVERICDR
                     VGVIIDGALIFEEPMSTFSEPNSLEERFFLEYAKVKGDLA"
     gene            2369..3517
                     /locus_tag="PK612_00500"
     CDS             2369..3517
                     /locus_tag="PK612_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH94635.1"
                     /translation="MKNLWTVIKKELKRFFTDKRMLAALILPGVIVYIHYSIMGNVMQ
                     STMEGKDHEYVIRVENRPNDPRFLAIETLIADPEVGFKKVTFLDETPEDFEAMIAEKK
                     LDLYVVYNTDFLAKYNANDPNDPPVVTFHYDESVIPSLNAYSTYAGLLNASLQTYVAV
                     PINHSAEGGIDTTIIKGLLPFLLITFIFTGAMAVAPESIAGEKERGTIATLLVTPVKR
                     STLALGKVTALSIVALVSAASSFLGVMLSLPKMMGSELSLATFTAGTYLAIFAVLIVS
                     MLVFIVLNSIISAYAKSIKEASGLAAPLMMVIMAVGVTTMFGSAATNNALYLIPIYNS
                     VNILLSLFGGEFLVVPFILTMLSNLLLTAGGVYLLTVMFSNEKIMFRK"
     gene            complement(3584..4102)
                     /gene="nrdG"
                     /locus_tag="PK612_00505"
     CDS             complement(3584..4102)
                     /gene="nrdG"
                     /locus_tag="PK612_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019000008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOH94636.1"
                     /translation="MTKKKIRIAGYVNDSLVDGPGIRFTIFTQGCPYHCLGCHNPDTW
                     DPKGGEEVEISTLIAKWRKNSLLDGITLSGGEPLLQKEALLPLINAARKDGLNITMYT
                     GSTYEELIHRNDPLVNEILSKVDYLIDGPFILHELNLNLLYRGSNNQRIIDLRKTTPN
                     NIVLINTMHDFK"
     gene            complement(4095..>6163)
                     /locus_tag="PK612_00510"
     CDS             complement(4095..>6163)
                     /locus_tag="PK612_00510"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOH94637.1"
                     /translation="LNTNYDGTIDIEVIQDEVEKFLMKKDPNIAKKYILYRKDRERIR
                     NAKSTVFNYKKLVEDYLNIADWRVKENSTVNYSLGGLILSNSGAVTANYWLSEVYDEE
                     VANAHRSAAIHIHDLSMLSAYCAGWDIEKIIRDGVGGVEGKISSKPAKHLFTLANQLV
                     NFLGIMQNEWAGAQAFSSFDTYLAPFVKADNLTYAEVKQAIQSFVFGVNTPSRWGSQA
                     PFSNITLDWTVPSDLADRPAIVGGKDMPFKYKDCQKEMDMINKAFLEVMIEGDANGRG
                     FQYPIPTYSITRDFSWEETENNKLLFEMTAKYGTPYFSNYINSDMEPSDVRSMCCRLR
                     LDLRELRKKGGGFFGAGSNTGSVGVVTLNMPRLAYLSRTEVEFFNELDKLMDISARSL
                     KVKRDVVSKLLEDGLFPYTKRYLGNFDNHFSTIGLVGMNEACLNARWLGKDLGDPESQ
                     AFTIKVLNHMREKLSDYQEAYGDLYNLEATPAESTAYRFAKHDKVRYPDIITARLGED
                     GDPFYTNSSHLPVGYTASISEALDIQDEMQTLYTSGTVFHTFLGQKLPNWQTAARLVR
                     KIAENYRLPYFTMSPTYSICPTHGYLTGEVEICPECKRTTEVYSRITGYYRPLKNWND
                     GKKAEFENRLEYADFTIEGGTVHNPKFNAPKVEVKDTADAPIKEQKPTPHKGAPIIFL
                     NKQNND"
BASE COUNT         1886 a         1167 c         1108 g         2002 t
ORIGIN      
        1 gggtcttgat ataaatgatg ggcataaatt ttaatatcgt atttttcttt agcctcttta
       61 acaatattta gtaactcttc taaagtaact atatttcttg gttctaaaga ttgtaagtct
      121 acatttgcac aaccaggaca tttaatattg cacccttgaa accatataca agatttacta
      181 tttgtaaaat caattgatcg gcttggtcgt gattacgata tgattattga agaatggcat
      241 catcttacta aagtacttga agctgatatc gttgttcttg atatgccact tcttgacaca
      301 cgaaacgatg cgactaattt agtgggtaag ttcattagtg atattgtttt acaaattctt
      361 tcctttgttg cggaaaccga aagagaattc atcaagcaga ggcaagcgga gggaataaga
      421 cttgctaaag aacgtggtgt gcatatggga agaccaccac tagtgttacc tgacaatttt
      481 gctgatgttg ttctcctctt tgataaagag aaaataacat gtacgaaagc tgcagcatta
      541 acaaatatgt cacgagcatc atttttgaag tatgcttcat cattttaaac agtaaaacac
      601 acttggcaac atttttatat tctagaatga taagggtttt cgccaaaaaa acataatttt
      661 acaaaaagcg gtatttaaga atatatacga ttttatgggt gtttttcaga tattgcagat
      721 aggttcgtac cactgtaacg gaaaattagt tttctagttc gaaccactgt agcggaaaca
      781 aaatttataa aaaaataagt tatgattatt gaaaatatca gtaaatttga gaaataaaaa
      841 tcatcaaaat taaagaaaaa ccgccatttt agacggtttt tgcagtgttt tagccgtttt
      901 ttacgcctta ctaacacaat atacgcttgg agcttccagt cggatttgaa ccaacggtca
      961 ttgagttgca gtcaattgcc ttaccacttg gctatggaag cgctactcaa ttatagcaaa
     1021 aagatttaat tattaaaact atgataaaaa gcgtactaca attaaacacc agattactaa
     1081 atttaaattg attttaaaca ttaatcttcc aatgctgcct ttaaatcaga aacggtatca
     1141 taccccttaa cattatcagt aagcgcaatc tttcttcctt cttcaattgc cgctttagtg
     1201 gtaacatttg aaacacttaa acttaaatga aatgggatgc ctttttcacg aattgtcgtc
     1261 cgtaaaaata tgttaacagc agtcgtcata ttgagtccca attcttgaaa tatcgcttct
     1321 gcttcttctt ttactttctt atccatccta atatttaaat tcgtcatgga catactttaa
     1381 ttcctcctat tcaatgtcat tttattatta tatcattaca ttgtcaatat gttgtcatta
     1441 ttttattaat taaattaagc gtttaataat taatcccact attaataaat acaatattac
     1501 ttcgcctaat ttctgtaaaa atctcgtaaa taattgtaat aacgtcaaaa tccaataatt
     1561 ctttatgtat aataaagaaa tgaggaggaa ctatgcaacc aatcttagaa gttaaaaatt
     1621 tagtaaagac ctttaaactt tcgaaaaaac aacaaaaaat cgaaaaaaca aatacgaaag
     1681 ttcgtgttgc ggttaatgac ctctccttta atgtttatga aggagaaata tatggtttac
     1741 ttggaccaaa tggcgcgggt aaaacaacaa cactacgcat catctcgacc ttaattaaaa
     1801 aagatagcgg tgaagttaaa gttaatggtt atgacattag taaagaacaa agtaaagttc
     1861 gcagtcaaat tggcttttta acgagtgaat taaagttaga cgacttcttc acacctaatt
     1921 acttatttga ttactttagt catcttcacg gtgttcctga agatgtgatt gaaaaacgta
     1981 aacaagaact tttcgataaa ttcggcatca gtgaatttaa agaagtaaaa gtaagtgacc
     2041 ttagtagcgg gatgaaacaa aaggtttcac ttgtcatcag tgtcgttcat gatccaaaat
     2101 tcattatctt tgatgagccg acaaacggcc ttgatgttat tactgcgaaa acagtcattg
     2161 atttccttgt cgcgctgaaa gaagcaggaa agactgttat tctttctacg cacatcttct
     2221 cactcgtcga aagaatatgt gaccgtgtcg gtgtcattat tgatggggcg ttaatctttg
     2281 aagaaccaat gagcacattt agtgaaccta attctcttga ggaacgcttc ttccttgaat
     2341 atgcgaaagt gaaaggagat cttgcctaat gaaaaactta tggactgtca ttaaaaaaga
     2401 attaaagcgc ttcttcactg ataaaagaat gctagcggcc ttaatccttc ctggtgttat
     2461 cgtgtatatt cactattcaa ttatggggaa tgtgatgcaa tcaacaatgg aagggaaaga
     2521 ccatgaatac gtcatccgtg tcgaaaatag accaaatgac ccgcggttcc tcgcgattga
     2581 aacactgatc gctgatcctg aagtcgggtt taaaaaagtt acgttccttg atgaaactcc
     2641 tgaagacttt gaagcaatga ttgcggaaaa gaaacttgac ttatatgttg tttataacac
     2701 tgatttttta gcgaaatata acgcgaatga tcctaatgat ccgccagttg ttacttttca
     2761 ctacgatgaa tcagtgatcc caagcttaaa tgcttatagc acttatgctg ggctcttaaa
     2821 tgcgtccctc caaacttatg ttgccgttcc cattaatcat tcggcagaag gcgggattga
     2881 tactacaatt attaaagggc tgctcccctt cttacttatt acctttatct ttaccggtgc
     2941 aatggcggtt gctcctgaaa gtattgctgg cgaaaaagaa cgcggtacga ttgcaaccct
     3001 cttagttaca ccagtgaaaa ggagtacttt agcgcttgga aaagttacag ctttaagtat
     3061 cgtcgccctt gtcagcgctg caagtagttt ccttggagta atgcttagct taccaaaaat
     3121 gatggggagt gaacttagtc tagcgacgtt taccgctggt acttacttag ctatctttgc
     3181 cgtcttaatt gtctcgatgc tcgtctttat tgtattaaat agcatcatca gtgcgtacgc
     3241 taagtcgatt aaagaagcta gcggactagc agcaccactc atgatggtaa ttatggcggt
     3301 aggggtcacc acgatgtttg gtagtgcggc aacgaataat gcgctctact taatccccat
     3361 ttataactca gttaatattt tactctcact ctttggtggt gaattcctcg ttgtaccctt
     3421 tatcttaaca atgctttcta acttactttt aactgcaggc ggagtttact tgcttaccgt
     3481 catgtttagc aatgaaaaaa taatgtttag aaaatagaaa aaacacttta taaacaaaag
     3541 gagtgacgcg tctcttgtca ctcctttttc attatggctt tctttactta aagtcatgca
     3601 ttgtattaat aaggacaatg ttattaggtg ttgtttttct taaatcaata atccgttggt
     3661 tattactgcc acgatagagt aaattaagat taagttcatg aagaataaaa ggaccatcaa
     3721 ttaagtaatc aactttactt aagatttcat tgacaagtgg atcgtttctg tgaattaact
     3781 cttcataagt tgaaccagtg tacatcgtaa tattaagtcc gtcttttctc gcagcgttaa
     3841 taagcggtaa gagtgcttct ttttgtaaaa gcggttcacc accacttaag gtaataccat
     3901 caagcagcga gttcttgcgc catttagcaa taagcgtact tatttcaact tcttctccac
     3961 ccttcggatc ccaagtatca ggattatgac aacctaaaca gtgataggga caaccttggg
     4021 taaaaatagt aaaacgaatc cctggtccat caaccaagga gtcgtttaca tatcccgcta
     4081 tgcgtatctt ttttttagtc attattttgt ttgtttaaga agatgattgg tgcaccttta
     4141 tgtggggtag gcttttgctc tttaattggt gcgtccgctg tgtcttttac ttcaactttt
     4201 ggtgcattaa attttggatt atgcactgtg ccaccttcaa tagtgaaatc agcgtattct
     4261 aaacgatttt caaattccgc ttttttcccg tcattccagt tcttaagcgg acgatagtat
     4321 ccagtaatcc gtgaatacac ttctgttgtt cttttacatt ctggacaaat ttcaacttca
     4381 ccagttaagt aaccatgtgt tggacagata gaataagttg gtgacattgt aaagtatggt
     4441 aagcgatagt tttcagcaat tttccgcact aagcgggctg cggtttgcca gtttggcaac
     4501 ttttgcccaa ggaaagtatg gaacacagtc cctgaagtat aaagtgtttg catttcatct
     4561 tgaatatcaa gcgcttcact aatacttgcg gtataaccaa ctggtaagtg acttgagttc
     4621 gtatagaaag gatcgccgtc ttcccctaac ctagcagtaa taatatcggg atagcgaact
     4681 ttatcgtgtt tagcaaaacg gtaggctgta ctttccgctg gtgttgcttc taagttatag
     4741 aggtcaccat aagcttcttg atagtcacta agtttttcac gcatatgatt aagcacttta
     4801 atcgtaaagg cttgtgattc tggatcgcct aagtcttttc ctaaccatct agcgtttaaa
     4861 cacgcttcat tcatccccac caaaccaatt gtagagaagt gattatcaaa gttacctaag
     4921 tagcgctttg tatatgggaa gagaccgtct tcgagtaatt tactaacgac atcgcgcttc
     4981 acttttaaac tgcgggcact gatgtccatt aatttatcaa gttcattaaa gaattcaact
     5041 tcagtacggg agagataggc taagcgtggc atgttaagtg tcacaacacc aactgaacca
     5101 gtattactac cagcaccaaa gaagccgcca ccttttttcc gtaattcacg taagtcaagc
     5161 cgtaagcgac agcacatact ccgcacatca cttggttcca tatcactatt aatatagtta
     5221 ctaaagtaag gagtaccata ctttgccgtc atttcaaata atagtttatt gttttctgtt
     5281 tcttcccatg agaagtcacg cgtaatggaa tatgttggga taggatactg gaatcctctt
     5341 ccgttagcgt caccttcaat cattacttca aggaacgctt tattaatcat atccatttct
     5401 ttttgacagt ctttatattt aaagggcata tctttcccac cgacaatcgc tggacgatca
     5461 gcgaggtcgc tagggacagt ccagtctagt gtaatattac taaacggtgc ttgactcccc
     5521 caacgactcg gtgtgttgac cccaaacaca aatgattgaa tagcttgttt tacttcagcg
     5581 taagttaagt tatcagcttt tacaaatggt gcaaggtaag tatcaaagct actaaaagct
     5641 tgagccccag cccattcgtt ttgcatgatg cctaagaaat taacaagttg attagcgagt
     5701 gtgaataagt gtttcgctgg tttacttgaa atctttcctt caacgccacc aacaccatca
     5761 cgaataatct tttcaatatc ccaacccgca cagtaagcac taagcatact taggtcatga
     5821 atatgaatgg cggcgctgcg gtgcgcattt gctacttctt catcatacac ttcacttaac
     5881 caatagtttg cggttacagc gccgctattg cttaagataa ggccacctaa agaataattg
     5941 acggttgaat tttctttaac gcgccagtcg gcaatattta aataatcttc aacgagtttt
     6001 ttatagttaa aaacggtgct tttagcgtta cgaatccgtt ctctatcttt tctatataaa
     6061 atatattttt tagcaatgtt gggatctttc ttcatcaaga atttttcgac ttcgtcttgg
     6121 atgacttcaa tgtcgattgt cccatcgtaa ttcgtgttta aat
//
LOCUS       DAOSEX010000012         6013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_11616_length_6013_cov_2.844747, whole genome shotgun sequence.
ACCESSION   DAOSEX010000012 DAOSEX010000000
VERSION     DAOSEX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11616_length_6013_cov_2.844747"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="PK612_00515"
     CDS             <1..1336
                     /locus_tag="PK612_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOH94638.1"
                     /translation="NKGVTVDRLLVEKLQRKMRNIVEVKLPFNRNKITIEEAEKLYEE
                     LGYKDKLEILKYRPEPTVHIYECDGYENYMYGYMVPSSGYLKNFIIRPYSPGMLLQYP
                     RSETKGEIPEFKDAPMFGKALKASHNWAKLTNLDSVAHINHYIENGHIVELINIAEAK
                     HYQMLSELGQRILEDSEDIRLICIAGPSSSGKTTFANRLRIELMSRGLNAIRLSIDDY
                     YLPRELAPRDEYGNYDLESIYALDIELFNKNMLDLINGEEVELPKFDFKKGQRVPGRK
                     LKVAANQPIIIEGIHALNEQLTSYIPKHQKYKIYIAPQAQINIDEHNPISLTDLRLLR
                     RIVRDKKYRNASAKETMAMWPSVRAGEFKWIYDTQEEADYVYNSLLSYELAVMKKHAL
                     PLLLEFDYLSEFFPIAERLIRMLKYFLDMDEKWVPSNSLLREFIGGSCFEDV"
     gene            complement(1387..2475)
                     /locus_tag="PK612_00520"
     CDS             complement(1387..2475)
                     /locus_tag="PK612_00520"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HOH94639.1"
                     /translation="MNDKKTYTPISNENFLRLLRFYKIPESAEDEVLYNLYIETVELL
                     TLHHQTFENIPYINLDHQRLILQLIHDYDFRMRGLNFEERRSLLKDELFHNKLINVVV
                     DKYGSSAIFKYDSGTYLTPFSMEISTINVYLNFIMLKLGSIPRHNKATELYAELLTSA
                     FSYVLTITELLVRGFEKEALATWRSLHELEATLLLIQDEKVLAQYNQHILYALAFNKL
                     IAQAESDKVFIEIKAKMKDLKLKSKDTKRFIEYGWLLAHNDFDLYIHKFNFRDGVQTL
                     AGLNHKRNIYQVASEVTHSSPLTLFTKRHYFLSIALENLYSSFLTIEALFAAFYIKNT
                     TKNEAEFYEVTRSIYLEDINFVKDRITK"
     gene            complement(2468..5593)
                     /locus_tag="PK612_00525"
     CDS             complement(2468..5593)
                     /locus_tag="PK612_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOH94640.1"
                     /translation="MKKETNKNTITFSPKQEEALSLNKENILVSAGAGSGKTAVLVER
                     IIRILIDKEVPLERILVLTFTRPGAQEFKNRIKKSLLENKTHAHKAFAVDNADITTFD
                     AYSLKILRKYGYLHPTMQSDITNLDANFEMVLMKQYFDEIMAEYYENPPLSLRYFATR
                     FLSKDDRQLFELIKSLDQQVNLQIDPEAYLDNYLDSFYSDTKFEEIYKELDAYVVTTI
                     RKVANAIKHFECERHLEAYETFLDHYGAILSFKDYMDNLDKFTYQRRPSGSLKEHPED
                     LPHAEEVKIYLNDLKEVQVFESKDRMYDVFSENKGHAAFVIEIYSQIRAKLAAYKKEK
                     QAYTFSDIARGAFELVSIPEIQHELKNQYDYILIDEYQDTSDIQEQFINKIANNNVYM
                     VGDIKQSIYGFRNANCDIFREKYARYRKNEGGKKIDLNDNYRSRREVLQSINAILSEI
                     MTEEYGGANYKKEHVINSGNLKYDEFKEERQKVGLEVINYVVPKNNKKNRDNNVLIRD
                     KIDYELDYIINDIEHKIVNKYQVYENKVGMRDVRYGDFTILIAQGTKFNRIEERFIRA
                     GIPVYVDRDEEANENNINIAINKLLTIYVAYTKSDEPKGFEFRLAVASFLRGFVNKYS
                     DQQLFDLLKDDNWDFSGDPILARIKKLAQTTKLLPVTEIIERLITEFDVYGALYNIRD
                     LTQNTMKLSSKLEEIENLAALGLDLEAIVNYFKEREIAGVNAALKRVKLEHDAVTIMT
                     IHRSKGLEFPIVYYMDLESNFYQVSRSQVMKVCPKYGFILPEPSYKSQKTFIGFLSNI
                     YTSKETLSEKIRLLYVALTRAKELMIVIHSTNEDGEPYKTFEKSSNLKQLLLQSTYYD
                     QKRLVVEPEDTQQRLSESEHSKEVKYIYSLHEHFYKFNKKPKTYTVSEIVDVDERLLA
                     RGTLLHRYFEEYDFTTKDLNYISDERDRQHMLNLLSLPFFANANANNIFREYEFIDDG
                     TNKTYAIDCFIIQDDKIMLFDFKLSNIYHPSYDVQVRNYAHYLQKVFKKSVEGYLISI
                     TDLKVRKVSNE"
     gene            complement(5574..>6013)
                     /locus_tag="PK612_00530"
     CDS             complement(5574..>6013)
                     /locus_tag="PK612_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94641.1"
                     /translation="KDYEVSALVLNSIKLTSTYSAGSRETFTEAIINDIKYTGILIDD
                     ADSLKILNAEADSERPFFKIGGRGKVKKLTDLIRREEYKNYMEVTRNKVEETYVGILN
                     NDFRIEDKKINNESSAKYSRYKEISFLPRNSVIEEADYEEGNE"
BASE COUNT         1962 a         1042 c          903 g         2106 t
ORIGIN      
        1 aaataaaggc gtcactgttg atcgattgct tgttgaaaaa ttgcaacgaa aaatgcgtaa
       61 tattgttgaa gtaaaattac catttaatcg caataaaata acaatagaag aagcagaaaa
      121 gttatatgaa gaacttggat ataaagataa actcgaaata ttgaaatatc gtccagaacc
      181 gactgtgcat atatatgaat gtgatggcta tgaaaactat atgtatggat atatggttcc
      241 ttcttcaggt tatttaaaaa actttataat taggccttat tcacctggaa tgctacttca
      301 atatccccgt agcgaaacaa aaggtgaaat tcctgaattt aaagatgcac caatgtttgg
      361 caaagcatta aaagctagtc acaactgggc taaattaaca aatttagata gtgtcgccca
      421 tattaaccac tatattgaaa atggccatat cgtcgaatta ataaatatcg ccgaagctaa
      481 acattaccaa atgttaagtg aattaggaca acgtatttta gaagattccg aagatattcg
      541 cttaatttgt attgcaggtc caagtagctc tggtaagaca acatttgcaa acagattacg
      601 cattgaacta atgagtcgcg gacttaacgc tattcgttta tcaattgacg actactactt
      661 accacgcgaa ttagcaccgc gtgatgaata tggcaactat gatttagaat caatttatgc
      721 ccttgatatc gaattattta ataaaaacat gctcgactta attaatggtg aagaagttga
      781 attaccaaag tttgacttta aaaagggtca acgcgtacca ggacgaaaac taaaagtagc
      841 ggctaatcaa ccaatcatca tcgaaggaat tcacgcactt aatgagcaat taactagtta
      901 tattccaaaa caccaaaaat ataaaattta tattgcacca caagcacaaa ttaacattga
      961 tgaacataac ccaattagtc ttactgattt aagattatta cgtcgtattg ttcgcgataa
     1021 gaaatatcgt aacgctagtg ctaaagaaac aatggcaatg tggccaagtg ttcgcgctgg
     1081 tgaatttaaa tggatttatg acacacaaga agaagccgat tacgtatata attcattgct
     1141 ctcttacgaa ttagcggtta tgaaaaagca tgcgttaccg ctcttgcttg aatttgatta
     1201 tttaagtgaa ttctttccta ttgcggaacg cttaatccgg atgcttaaat atttccttga
     1261 tatggatgaa aagtgggtac cttcaaattc attactccgt gaatttatcg gcggcagctg
     1321 ttttgaagat gtataatcat taataattgc taactcttga gccactttta accgagtggc
     1381 tcttttttat tttgttatgc gatcttttac aaagttaata tcttctagat aaatagaacg
     1441 cgtaacttca taaaattccg cttcattttt tgtggtgttt ttgatgtaaa acgctgcaaa
     1501 aagcgcttca attgttaaaa atgagctgta tagattttca agagcaatac ttaaaaagta
     1561 atgtcgttta gtaaataaag taagtggtga actatgtgta acttcactag caacttgata
     1621 aatattgcgt ttatgattta gtccagcgag cgtctgcacc ccgtcacgaa aattgaactt
     1681 atgaatgtat aaatcaaaat cgttgtgcgc taaaagccag ccgtattcaa taaatctttt
     1741 cgtatcttta cttttaagtt ttaagtcttt catcttcgct ttaatttcaa taaaaacttt
     1801 atcgctttct gcttgtgcga taagtttatt aaacgctaag gcgtaaagaa tatgttgatt
     1861 atattgagcg aggacctttt catcttgaat taagagtagt gttgcttcta attcatgcag
     1921 tgagcgccat gtagcaagcg cttctttttc aaaaccgcgc acgagtaatt ctgtaatcgt
     1981 aaggacatag gaaaaagcac ttgtcagtaa ttccgcatat agttctgtcg ctttattatg
     2041 gcgtggaatc gatcctaatt taagcattat gaaatttaaa taaacattaa ttgtcgatat
     2101 ttccatagaa aacggagtta aataagtgcc agaatcatac ttaaaaatag cgctgctccc
     2161 atatttatca acaacaacat taatgagttt gttatgaaaa agttcatctt taagtagtga
     2221 ccgacgttct tcaaagttaa gaccacgcat ccgaaaatca taatcatgaa taagttgtaa
     2281 tattaatcgc tgatgatcaa gattaatata cggtatattt tcaaaagtct gatgatgtaa
     2341 tgtgagaagt tcaacggttt ctatgtataa attgtataag acttcatctt ccgctgattc
     2401 tggaatttta taaaaccgaa gcaaccgcaa aaagttttca ttacttatcg gtgtatatgt
     2461 ttttttatca ttcatttgat actttcctca cttttaagtc agtaatacta attaaatagc
     2521 cttcaacact tttcttaaaa accttttgta aataatgtgc atagtttctt acttgaacat
     2581 cataacttgg atgataaata tttgaaagtt taaaatcaaa aagcattatc ttatcatctt
     2641 gaatgataaa gcagtcaatc gcataggttt tatttgttcc atcatcaata aattcatatt
     2701 cacggaatat attattcgca ttagcattag cgaagaaagg taaacttaac aggttaagca
     2761 tatgttgtcg atctctttca tcactaatat aattaagatc tttcgtagta aaatcatatt
     2821 cttcaaaata acgatggagc aaagtaccac gcgctaaaag cctctcatca acatcaacta
     2881 tttcactaac cgtgtaagtt ttaggctttt tattaaattt ataaaaatgt tcatgaagcg
     2941 aataaatgta tttaacttct ttactatgtt cactctcgct aagtcgctgt tgtgtgtctt
     3001 caggctcaac tactaaacgc ttttgatcat agtaagttga ttgaagaagg agttgcttaa
     3061 ggttactaga cttttcaaaa gttttataag gttctccatc ttcattagta gaatgaatga
     3121 caatcatcaa ctctttggca cgtgttaggg caacataaag taatctaatt ttctcggata
     3181 atgtttcttt agatgtatat atatttgaca agaaaccaat gaaagtcttt tgggatttat
     3241 aggacggttc gggtaaaata aaaccgtact ttggacatac cttcatcact tgactcctac
     3301 ttacttgata aaaattagat tcaaggtcca tataatagac aatcggaaat tctagaccct
     3361 ttgaacggtg gattgtcata atcgttacag catcatgctc taattttaca cgttttaagg
     3421 cagcattgac tcccgcaatc tcacgttctt taaagtaatt tacaatagct tctaaatcta
     3481 aacctaatgc cgctaaattt tctatttctt ctaatttact cgacaatttc atagtatttt
     3541 gcgttaaatc tcgaatattg tatagtgcac catatacatc aaattcagta atgaggcgct
     3601 caataatttc tgttacgggt aagagtttag tggtttgtgc tagcttttta atgcgtgcta
     3661 gtattgggtc accactaaaa tcccaattat catcttttaa taaatcaaaa agttgttggt
     3721 cgctatattt attaacaaat ccgcgtaaga aactagcgac cgctaaacgg aattcaaaac
     3781 ctttcggctc atcacttttt gtgtaagcaa cataaatagt tagtaattta tttatcgcga
     3841 tattaatatt attttcattt gcttcttcat cacgatcaac ataaacagga attcctgcgc
     3901 ggataaagcg ttcttctatt cgattaaatt tcgttccttg cgcaatcaaa attgtaaagt
     3961 caccataacg cacatcacgc ataccgactt tattttcata aacctgatat ttattaacta
     4021 ttttatgttc gatgtcatta atgatgtaat caagttcata atctatcttg tcacggatta
     4081 aaacattatt atcacgattt ttcttattat ttttaggcac aacataatta attacttcta
     4141 atcctacttt ttgtctttct tctttgaatt catcatattt aagattgccc gaatttatca
     4201 catgttcctt tttatagtta gcgcccccat attcttcagt cattatttca cttaaaatag
     4261 cgttgattga ttgtaaaact tctctacgac ttcgataatt atcatttaaa tcaatctttt
     4321 taccgccttc atttttgcga tatctagcat atttttcacg gaatatatca caattagcgt
     4381 ttctaaaacc ataaatactc tgttttatat caccaaccat ataaacatta ttattggcaa
     4441 ttttatttat aaattgttct tgaatatcgc ttgtatcttg atattcatca atcaaaatat
     4501 aatcatattg atttttaagt tcatgttgaa tttcaggaat actcactaac tcaaaagcac
     4561 cgcgagcgat atcactaaaa gtataagctt gtttttcctt tttatacgct gcaagttttg
     4621 ctctaatttg cgaatatatt tcaataacaa acgctgcatg acccttattt tcactaaaga
     4681 catcatacat tctatctttt gattcaaaaa cttgtacttc ctttaagtca ttaaggtata
     4741 tcttaacttc ttcagcgtgc ggtaaatctt caggatgctc ttttaatgag ccacttggcc
     4801 gtcgctgata agtaaattta tcgaggttat ccatataatc tttaaacgat aatatcgcac
     4861 cataatggtc tagaaatgtt tcataagctt ctaaatgtct ttcacattca aaatgcttaa
     4921 tggcattagc gactttacgt atcgtagtta caacataagc atctagttct ttataaattt
     4981 cttcaaattt agtatctgaa tagaaggagt ctaaataatt atcaaggtaa gcttctggat
     5041 ctatttgtaa attaacttgt tggtctaaac ttttaattag ttcaaataat tgtcgatcat
     5101 ccttactaag aaacctagtc gcaaaatagc gtaatgaaag cgggggattt tcataatatt
     5161 cagccataat ttcatcaaaa tattgtttca ttaacaccat ttcaaaatta gcgtctaaat
     5221 tagttatatc actttgcatt gtggggtgga gataaccata tttacgtaat atttttaagg
     5281 agtaagcatc aaatgttgta atatctgcat tatcgaccgc aaaagcttta tgtgcatgcg
     5341 ttttattttc aagcaaggat ttttttatcc gatttttaaa ctcttgcgct ccaggtctag
     5401 taaaggtaag aacaagaatc cgttctaaag gaacctcttt atcaattaaa atacgaataa
     5461 tacgttcgac taaaacagca gttttaccac taccagcacc agcagaaaca agaatatttt
     5521 ctttgtttaa actaagagct tcctcttgtt tcggactaaa tgttattgtg ttcttattcg
     5581 tttccttctt cataatctgc ctcttcaata acactgttcc tcggtaagaa actaatttct
     5641 ttatagcgtg aatatttcgc gctactttcg ttatttatct ttttatcttc aattctaaaa
     5701 tcattattta atatacccac ataagtttct tcgactttat tacgcgtcac ttccatataa
     5761 tttttatatt cttctctacg gattaagtcc gtaagttttt taactttccc gcgtccacct
     5821 attttaaaaa atggtcgctc actatctgct tcagcgttta atatttttag gctatcagcg
     5881 tcatcaatta aaataccagt gtatttgatg tcattaataa ttgcttctgt aaatgtttct
     5941 cgactacccg cactatatgt actagtaagt ttaatggagt ttaatacaag agcactaact
     6001 tcataatcct tat
//
LOCUS       DAOSEX010000013         5841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_12083_length_5841_cov_3.405980, whole genome shotgun sequence.
ACCESSION   DAOSEX010000013 DAOSEX010000000
VERSION     DAOSEX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12083_length_5841_cov_3.405980"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..646
                     /gene="rplM"
                     /locus_tag="PK612_00535"
     CDS             191..646
                     /gene="rplM"
                     /locus_tag="PK612_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOH94642.1"
                     /translation="MLRQTTHANAATVERKWYVVDATDLPLGRLASEIAMTLMGKNKP
                     EYTPHVDCGDYVIVVNADKVSLSGNKLKTKKYYNTSQRPGGLRVRTAGEMLANYPVEM
                     MERAVWGMVPKGRLGRQIIKKLFVYAGPEHKHSAQQPVELPLRYIGGSK"
     gene            646..1044
                     /gene="rpsI"
                     /locus_tag="PK612_00540"
     CDS             646..1044
                     /gene="rpsI"
                     /locus_tag="PK612_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOH94643.1"
                     /translation="MAKRKAETYLGTGRRKSSVARIILKPGKGSIIVNNQDVNDYMPY
                     QTLVMDLKQPLTLLDVADKYDVIANVHGGGFTGQAGAIRLGIARALLEAGLDRGTLKR
                     AGMLTRDARAKERKKYGLKGARRAPQFSKR"
     gene            1174..1250
                     /locus_tag="PK612_00545"
     tRNA            1174..1250
                     /locus_tag="PK612_00545"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1208..1210,aa:Ile,seq:gat)
     gene            1356..3257
                     /locus_tag="PK612_00550"
     CDS             1356..3257
                     /locus_tag="PK612_00550"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HOH94644.1"
                     /translation="MKQELIKNNYYEAVNKEWIDAAVIPGDQPMMSAFLELHLDIEKL
                     LMDLAKKWDKDQTGLNNHLQEFVKLYKMTNDFATREKLGAEPLKPVIKKILNLKSLKD
                     LEKNMKDFVLDGVDLPFSFMVFQDFKDSNNEVLYFTAANLFLPDTSYYENEETKQQLI
                     GLFTMTTMQVLSLYGLEEEAAQDLLNKALAFDAMLVPVTMSSVERADMIKMYNPLLKD
                     EFASKTKTFNLLKVAEALVGQDVDKVVAMNLDFIDKFEEIVNDANFELIKAWMVVSNI
                     LTFSAELTEELRVAGGAFGRALQGIAEAQSQEKAAFYQAYDRLSQAVGLYYGLTYFGP
                     EAKKDVEDMVNTIIGVYKDRINKNTWLNAETKAKAILKLNTMKVHIGYPRELPKYYDK
                     YKFGSYAEGSNLVKEKLKYSRLVQKENFKKYNKKPNRNLWAMPASMVNAYYQPMNNQI
                     VFPAAILQEPYYSLSQGRSANYGGIGAVIAHEISHAFDNNGAKFDEYGSLNNWWTDED
                     LKTFEEKAQKMIALFDGVESGYGPCNGTLTVSENIADSGGIRCALEASKKEKDHNYEE
                     FFKNWARVWRQKSSPEFMQLLLTVDVHSPSNLRANMQLKNLVEFQEFYNLTEQDEMYL
                     AKEQMVEIW"
     gene            3352..3427
                     /locus_tag="PK612_00555"
     tRNA            3352..3427
                     /locus_tag="PK612_00555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3385..3387,aa:Val,seq:cac)
     gene            3438..3513
                     /locus_tag="PK612_00560"
     tRNA            3438..3513
                     /locus_tag="PK612_00560"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3471..3473,aa:Thr,seq:tgt)
     gene            3520..3604
                     /locus_tag="PK612_00565"
     tRNA            3520..3604
                     /locus_tag="PK612_00565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Leu,seq:tag)
     gene            3712..3903
                     /locus_tag="PK612_00570"
     CDS             3712..3903
                     /locus_tag="PK612_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH94645.1"
                     /translation="MINYAQKIREYRERKFLTQTEFAALLGVSIASVTRWETGHYQPT
                     MKVKKKIYNLLVEAGIEIE"
     gene            4079..4933
                     /locus_tag="PK612_00575"
                     /pseudo
     CDS             4079..4933
                     /locus_tag="PK612_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214884.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
     gene            5091..5600
                     /locus_tag="PK612_00580"
     CDS             5091..5600
                     /locus_tag="PK612_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOH94646.1"
                     /translation="MCQKTFEEKLAELDACIEKHQKSPGPLMPTLHDAQEIFGAIPLE
                     IQKHISKKLGVSVAHINGVVTFYSRFSLTPKGKYIISVCMGTACYVKGAQAILDAVQS
                     ELKLKPGETSEDGLFTLEATRCIGACALAPVYSINDEIYGGGTSNKILEDLRKIIKAE
                     EEKNDQNKA"
     gene            5581..>5841
                     /locus_tag="PK612_00585"
     CDS             5581..>5841
                     /locus_tag="PK612_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94647.1"
                     /translation="MIKTKRDLEVIKKRMQPDFHLRKEEHKYRLLFRYNEKTVIQNIE
                     KIIDLCLAAIYQDKLDAVAIKVYDTRVEDKLILVVKEKNRAVR"
BASE COUNT         2098 a          907 c         1190 g         1646 t
ORIGIN      
        1 atcattctta gcaagaaaat tacgaaacgc gaaggttaat ttcatcttta tgtcggctta
       61 tcaattatat tgataaataa aataacgaat taagttgaca atatatttat aatatattac
      121 tattaatcac ggcattatct gccttttgtc gtcccggtaa gacgaaaatt ggtaaaggag
      181 aaatacaaat atgctacgtc aaacaacaca tgcgaatgct gcaaccgttg aacgtaaatg
      241 gtatgtcgtt gacgctactg atcttccact cggtcggtta gcgagcgaaa tcgccatgac
      301 gctcatgggc aaaaacaaac cagaatatac cccacatgtt gattgtggag attatgttat
      361 cgttgttaac gctgacaagg ttagtttaag cggcaataag ctcaaaacaa agaaatatta
      421 caacaccagc caacgtcctg gcggtttacg cgtgcggaca gcaggtgaaa tgctcgcaaa
      481 ttatcctgtc gaaatgatgg aaagagcagt gtggggcatg gttccaaaag gtcgtcttgg
      541 ccgtcaaatt attaagaaat tatttgtcta tgctggacca gaacacaaac atagtgcaca
      601 acaaccagtg gaactaccct tacgttatat tggaggaagt aaataatggc aaagagaaaa
      661 gcagaaactt acttaggtac tggccgtcgt aaatcaagcg tcgcacgcat tattttgaaa
      721 cctggtaaag gttcaattat tgttaataat caagatgtta atgattacat gccttatcaa
      781 accttagtta tggacttaaa acaaccatta actttacttg atgttgccga caaatatgat
      841 gtcatcgcta acgttcatgg tggtggcttt accggtcaag caggggcaat ccgtttagga
      901 attgcgcgtg ctttacttga agctggcctt gatcgcggta cacttaaacg cgcgggaatg
      961 ttaacccgtg atgctcgtgc caaagaacgg aagaaatacg gtcttaaagg cgctcgtcgt
     1021 gcaccacaat tctccaaacg ttaatataac tacacgagaa caaacgaaag tctggttcaa
     1081 aatgccagac tttttcttat attaaaaact tttaatgtta aaaaagttgc aatatgatca
     1141 atgttgttat attatatatt agcgtccgca gatgggccct tagctcagtt ggttagagcg
     1201 cacccctgat aagggtgagg tcgatagttc aaatctatta gggcccacca tttgcaagta
     1261 aagtcactac tttgtagtga tttttttgta aagaataagt aaacttcctc tatttaccat
     1321 acataaatgt taaaatacga tggtgaggtg aaaatatgaa acaagaacta attaaaaaca
     1381 attattacga agctgttaat aaagaatgga ttgatgccgc agtaattcct ggtgatcaac
     1441 caatgatgtc agcattttta gaactacatt tagatattga aaagttatta atggatttag
     1501 cgaaaaaatg ggataaagac caaacaggat taaataatca tctgcaagaa ttcgttaagc
     1561 tttacaaaat gacgaatgat tttgctacaa gagaaaagct tggtgctgaa ccacttaaac
     1621 cagtaattaa gaaaatctta aatttaaagt cacttaaaga cttagaaaaa aatatgaaag
     1681 attttgtgtt agatggagtg gatttaccat ttagctttat ggtgttccaa gattttaaag
     1741 attcaaataa cgaggtgcta tattttaccg ctgcgaattt attcttacca gatacttcat
     1801 attatgaaaa cgaagaaacg aaacaacaac ttattgggtt atttacaatg acgacaatgc
     1861 aagtgctaag tttatatggt ttagaagaag aagcggcaca agacttatta aataaagcgt
     1921 tagcgtttga cgcgatgcta gtgccagtga cgatgtcgag tgttgaaaga gccgacatga
     1981 ttaaaatgta taacccatta ttaaaagatg aatttgccag taaaacaaaa acatttaatt
     2041 tactcaaagt tgcggaagca ttagtcgggc aagatgttga taaagtagta gcaatgaatt
     2101 tagactttat tgataagttt gaagaaattg ttaatgacgc taattttgaa ctaattaaag
     2161 cgtggatggt tgtctctaac atccttacat tcagcgctga attaacggaa gaattacggg
     2221 ttgctggtgg tgcttttggc cgcgctttac aaggtattgc ggaagcacaa tcacaagaaa
     2281 aagcagcgtt ttatcaagct tatgaccgtc tttcacaagc tgttgggctc tattacggct
     2341 taacttactt tgggccagaa gctaaaaaag atgttgaaga tatggttaat actattattg
     2401 gtgtttataa agaccggatt aataaaaaca catggcttaa cgctgaaaca aaagcaaaag
     2461 caattcttaa acttaacacc atgaaagtac atattggtta tccgcgtgaa ttaccaaaat
     2521 attatgataa atacaaattt ggcagttacg ctgaagggtc aaatttagtg aaagaaaaac
     2581 ttaaatatag ccgtttagtg caaaaagaaa actttaagaa atataataaa aaaccaaatc
     2641 gtaatttatg ggcaatgcca gcttcaatgg ttaatgctta ctatcaacca atgaacaatc
     2701 aaatcgtttt ccctgctgcg attttacaag aaccatatta tagtttaagt caaggtcgta
     2761 gcgctaatta cggcggaatt ggtgcagtta ttgcccatga aatctcgcac gcctttgata
     2821 ataatggcgc aaagtttgat gaatacggct cacttaataa ctggtggaca gatgaagatc
     2881 ttaaaacatt tgaagaaaaa gcacaaaaaa tgattgcatt atttgatggt gttgaatcag
     2941 gatacgggcc gtgtaatggt actttaaccg tttccgaaaa catcgctgat agtggcggaa
     3001 tccgctgtgc cttagaagca agcaaaaaag aaaaagacca taattatgaa gaattcttta
     3061 aaaattgggc gcgtgtctgg cggcaaaaat ctagtccaga atttatgcaa ttactcttaa
     3121 ctgttgatgt gcatagtcca tcaaacttac gtgcaaatat gcaacttaaa aacctcgttg
     3181 aattccaaga gttttataat ttaacggaac aagacgaaat gtatttagca aaagagcaaa
     3241 tggttgaaat ttggtaaaca atcaaatctt gtaaactaag agggcttgtc cctctttttt
     3301 aattgctatt tataaatgat tttgctatta tattacaagg tcgtcccttc cggacactta
     3361 gctcagatgg gagagcgttt ccttcacacg gaagaggtcg ggggttcgat cccctcagtg
     3421 tccaccattt atttaacgcc gacgtggctc aattggcaga gcaactgatt tgtaatcagt
     3481 aggttgtagg ttcgagtcct atcgtcggca ccattacttg caaacgtggc ggaactggca
     3541 gacgcgctag acttaggatc tagtggggaa acccatgcag gttcaattcc tgtcgtttgc
     3601 accacgttac ttaaattgct ccttaaacgg ggcatttttt attaattcca atagcgttag
     3661 tgataaaaaa gtgataaaat aatgtgtaaa tagtttaaat aggagtttat catgattaat
     3721 tatgcccaaa agataagaga gtatcgtgaa cgaaagtttt tgacacaaac agaattcgcg
     3781 gcgcttcttg gtgtttctat cgcatcagtg acgagatggg aaacaggaca ttatcagcca
     3841 acaatgaaag ttaagaaaaa aatatacaat ttattagttg aggctggaat tgaaatagaa
     3901 taaaaggaga ttaactatta gaggttaaga aaaaagataa gagtatagta aaaaaactcc
     3961 acaaggctgt aggtaaaatt aacaagtaaa tagtgtaatt gttatacccg gagaaagagg
     4021 agaatattat ctaatcatta attaaaccgg gtaggattag ataatatgaa attaagtttt
     4081 gaaaaacgaa attaaattat ttgaaggcaa acaaattaga acacattggg atgaagaaaa
     4141 agaagaatag tattttagtg ttgtagatat cattggcgtc ttaacagaga gcaaagaccc
     4201 cggagcatac tggagaaagc taaaacagcg attaaaagaa gacggaagtg aaaccgtgac
     4261 attttgtcac gctttgaaat tgaaagcatc agatgggaaa atgcgtaaca ctgatatcgc
     4321 tgatatacga ggaatttttc gcatcataca atctgttcca tcgcctaaag ccgagccttt
     4381 caagatgtgg ttagcggaag taggaaaaga gcgggttgat gaaataatgg atccagagct
     4441 aactattgat cgtgctttac gcacatattt agcaaaaggt tacacccgtg aatggattaa
     4501 tcaaagatta caagcaatat cggtacgtaa agagttaaca gatgcgtggc aagatcatgg
     4561 tgttaaagct ggttcagaat acgcaatatt aagaaatgaa attacaaaag cctggagtgg
     4621 aatgacaact aggcaatata aaaatctaaa aggtttaaca aaagaaagtt tacgagataa
     4681 tatgtcaaca acagaaattg tgttaaatat gcttgcagaa acggccacta aagaaattat
     4741 taatgtaaag catcctgatg gacttgaaga aaatatcgag atagcaaatg agggtgggag
     4801 tgttgccaaa gcggcgcgcg aagcaattga gtcaaaaact ggaaaaaaag ttataacgag
     4861 taaaaaagca cttgatttag gaagagtaat taccgctata accgaaaaaa atgatgatga
     4921 taatgaaaaa taataagata atgcgttgta attgacatgt acgcttttta agaatatcta
     4981 agatttttca atgtctcatt aaaaaaacat ttatgatatt gttttgaatt tataatattc
     5041 atattataat catagttgta tctggaaata aataccgata ggagacgaat atgtgccaaa
     5101 aaacatttga agaaaaactt gcggaacttg atgcttgtat tgaaaagcat caaaagagtc
     5161 cagggccact gatgcccaca ttacatgatg ctcaagaaat atttggagca attcccttag
     5221 aaattcaaaa gcatattagt aagaaattag gcgtgagtgt cgcccacatt aatggtgttg
     5281 tgacatttta ctcacgtttt tctttaaccc cgaaaggaaa atacattatt tcagtttgta
     5341 tggggacagc gtgttatgtt aaaggcgcgc aagctatact tgacgctgtg caaagtgaat
     5401 taaaacttaa accaggcgaa actagtgaag acggtttatt tacgcttgaa gcaacgcgct
     5461 gtattggtgc atgtgcgctt gcccctgttt attcaattaa tgatgaaatt tatggcggtg
     5521 ggacaagtaa taaaattctt gaagacttac gaaaaataat taaagcagag gaagaaaaaa
     5581 atgatcaaaa caaagcgtga tttagaagta attaagaaaa gaatgcaacc agattttcat
     5641 ttacggaaag aagaacataa atatcggtta ttgtttcgtt ataatgaaaa aacagtaatt
     5701 caaaatattg aaaaaataat tgatctctgt ttagcggcta tttatcaaga taaacttgat
     5761 gcagtagcaa ttaaagttta tgatacacgc gttgaagata aattgatttt agttgttaaa
     5821 gagaaaaatc gtgctgttcg t
//
LOCUS       DAOSEX010000014         5785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_12244_length_5785_cov_3.991798, whole genome shotgun sequence.
ACCESSION   DAOSEX010000014 DAOSEX010000000
VERSION     DAOSEX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12244_length_5785_cov_3.991798"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PK612_00590"
     CDS             complement(<1..456)
                     /locus_tag="PK612_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94648.1"
                     /translation="MKKRRLLIPLIGLALLLTGCKTTSSEEKPVSSEQPPVTSVTTEQ
                     PPVSSEEPPASSEETPVANIKFGADPFNGTSATGVAGVGVALYYDATEDNVAVAFDKV
                     AFTVNKPSGVLFDPVIGLVEKLSVYYLRPMEPGTYTVTLTVEDSAGLKAT"
     gene            complement(496..1953)
                     /locus_tag="PK612_00595"
     CDS             complement(496..1953)
                     /locus_tag="PK612_00595"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HOH94649.1"
                     /translation="MFCYHKTSASNNEKFFRKGIFLSITLLIATTVLTSCKGGTSVNS
                     EIRSSEFNNNEVVYPSNPAFGNNVTAHDPSIFKDDDGTYYTFGSHFAVTKSINLMQWT
                     QVANDGGANVLYARNWRTVLADAFAHVGERPGSTWAPCVHKFGEKYYMYYSLSTFGSS
                     VSYIGRVEGDSVLGPYDNSVEIVKSDGRGGPNAIDPELFFSKDGRLWLVYGSFFAGIY
                     IKELYASGENIGLPKEDGYGKLLWQGGDQGPEGPFIFYNPNTDYFYLMASHGSLSTNY
                     NMRVARSKNPDGPYTDPRGMSAENYRNSGLKLAGNYQFQGYPIGYAALGHNSILREDG
                     KYYVVYHSRYRSGASGVTGYHNQFVNQMFFNKNGWPVLAPNRYAGEELQVITMAQASK
                     DYDVLIHTGGDSEGFATSVTYKFSQNGDLLDAEEDAIGTWSINEDYYVTIKIGLNTYE
                     GVIIPQWNNDLQRAGLSITAYSRNGLSLWANEHFR"
     gene            2238..3245
                     /locus_tag="PK612_00600"
     CDS             2238..3245
                     /locus_tag="PK612_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH94650.1"
                     /translation="MPTIRDIARKANVSPATVSRVLNGDPTINVQAKTKRRIFEVAED
                     LAYTKHLRPKKNQQKNKFRIAIYHWYTIEQEINDPYFISIRIGVQRACRELGIAFDVI
                     YREDRITDPFEDQKYDGVIMIGSFTRETQTFISDRFSAVVYANSEDEDGKCDCVRVDF
                     RALTHDVLHLLINKGHKKIAYIGGRERVSSEPNKTPDPRELEYIEVMHQNKLYYKPYV
                     KVGEYSAHSGYEPANELIDNNEGNTPTAIFCGNDSIALGVSKAIQERGFKIPDDIAIF
                     GVNDIPTLQFTSPSLSSVKIHTEFMGELALKLLNERMKKERSLKLSVNVPYELILRES
                     A"
     gene            complement(3247..3792)
                     /locus_tag="PK612_00605"
                     /pseudo
     CDS             complement(3247..3792)
                     /locus_tag="PK612_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            complement(3804..4664)
                     /locus_tag="PK612_00610"
     CDS             complement(3804..4664)
                     /locus_tag="PK612_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOH94651.1"
                     /translation="MKETNKKTRLKGFMKTTVLSFQHMFAMLGATVLVPMLAGLSISI
                     TLITAGIGTIIFYFVTKRKVPVFLGSSFAYLPALMSIMAESGEVGSLAWQQNMGKLVI
                     AIFFTGVMYLIFAGVIRLIGVEKVRKMFPPRVVGPVIIVLGMLLVPSIFWNNIIAPYA
                     LYGAEAWKVWTTALITLLSIIAISAFAKPKSFFKAAPILLGFLIGYVYGAIVGLVDFS
                     TLKGSDIVIFQSIGSLLGFYKYLKFDWNAILMMVPIAFVTLMEHIGDISANSTVCGKD
                     FFVDPGIDRT"
     gene            complement(4706..5206)
                     /locus_tag="PK612_00615"
     CDS             complement(4706..5206)
                     /locus_tag="PK612_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOH94652.1"
                     /translation="MEKWDLYTKDEKKTGKIVDKGALIPEGYYHIIVETLIKHTDGTY
                     LVMQRSYDKKEYPGKYEGSCGGSIKAGETKEEGAIREVFEESGLVINEVTPTYYFIQK
                     DVPAINQGYLAIISAKDKTIKYQKEETINHKWLTLKELKAFIKSDEYNESHASRLVTY
                     LDSLNE"
     gene            complement(5373..>5785)
                     /locus_tag="PK612_00620"
     CDS             complement(5373..>5785)
                     /locus_tag="PK612_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94653.1"
                     /translation="KNGRNITVIYSINKDAFIAMMESGLRGILFSEEMTSEERAEAEE
                     FLQWYVALLEEVLDLREYKITIGADGNDHLTQVRADFELITTMVYEPSETYPEGYTQV
                     GKLSGFLDMKPKFNEEFTITLPDFSDYEEGIIFE"
BASE COUNT         1805 a         1188 c          929 g         1863 t
ORIGIN      
        1 agttgctttt aatcctgcgc tatcttcaac tgtcagtgtt accgtatatg ttcccggttc
       61 cattggtcgt aaataataaa cactaagttt ttccactaaa ccaattactg gatcaaagag
      121 aacacctgat ggtttattaa cagtaaatgc cactttgtca aaagcgacag cgacattgtc
      181 ttcagttgcg tcgtaataaa gcgcaacacc aacgcccgcg acacctgttg cggaagttcc
      241 gttgaacggg tcagcaccaa atttaatgtt agcaactggc gtttcttccg aacttgctgg
      301 tggttcttca cttgaaaccg gtggttgttc tgtagtaacg cttgttactg gtggctgttc
      361 cgaacttact ggtttttctt cacttgatgt tgtcttacat cctgtaagca gaagtgctaa
      421 accaataagt ggaataagta gtcttctttt tttcatatta ttctcctttt ttattagcct
      481 tttttggctt tataattatc taaaatgttc gttggcccat aaacttaggc cattgcggct
      541 ataagccgta attgaaagtc cggctcgttg aagatcgtta ttccattgtg gaataatgac
      601 accttcatat gtatttaagc caattttaat cgtgacataa taatcttcgt taatgctcca
      661 tgtaccgata gcatcttcct cagcatctag caagtcaccg ttttggctaa acttataagt
      721 tactgatgta gcaaaccctt cactatcacc ccctgtatgt attaatacat cataatcttt
      781 tgacgcttgt gccattgtaa taacttgaag ttcttcgccc gcatagcgat ttggtgcaag
      841 taccggccaa ccatttttgt taaaaaacat ttgattgaca aattgattat gataacccgt
      901 cacacctgat gcgccactac gataacgcga atgataaaca acataatatt ttccgtcttc
      961 tcttaaaatc gaattatgac cgagtgcagc ataacctatt ggataacctt gaaattgata
     1021 attaccagcg agttttagac ctgaattacg ataattttca gcactcatac cgcgcgggtc
     1081 agtataagga ccgtctgggt tcttactgcg cgcaacacgc atattataat tagtagataa
     1141 ggaaccatgc gatgccatta aatagaaata atcagtgtta ggattataga aaatgaaagg
     1201 accttctggg ccttgatcac caccttgcca taaaagcttg ccgtagccat cttcttttgg
     1261 taacccaata ttttcgccgc ttgcatataa ttctttaata taaatacccg caaaaaatga
     1321 accataaact aaccataatc ttccgtcttt actaaagaat aattcaggat caattgcatt
     1381 tggtccaccg cgaccatcag attttacaat ttccacagaa ttgtcgtatg gaccaagcac
     1441 actatctcct tcaacacgac caatataaga aacactacta ccaaaagttg ataaagaata
     1501 gtacatataa tatttttcac caaacttatg aacacaaggt gcccaagtac taccgggtct
     1561 ttcaccaaca tgagcaaaag cgtccgctaa tacagtgcgc cagttacgag catataaaac
     1621 attagcgcca ccatcattag caacttgggt ccattgcatt aagtttattg atttagtaac
     1681 agcaaaatga gaaccaaaag tataataggt cccatcatca tccttaaaga ttgaaggatc
     1741 atgtgccgta acattattac caaaggcagg attagacgga taaactactt cattattatt
     1801 aaattcacta ctccgtattt cgctattcac gcttgtacct cccttacacg atgttaaaac
     1861 tgttgtcgct attaaaagag ttatcgatag gaaaatacct ttacgaaaaa acttctcatt
     1921 attactagcg ctagttttat ggtaacaaaa catagattcc tccttaggtt catctaatcg
     1981 ttatgaaata tgtggtaatt atcttaccta cagattatca catgtttact aaatgtcaag
     2041 cgtaatttat ttctggctaa aacgaaaatt tgcgttgtaa attctttgtt ttttaacata
     2101 gttcatataa aatcaatgtt atttgttgcg cagtttaaaa ttgtttactg tttgtttagt
     2161 aaatatttta atttaattgt tgatatttac cagaccttat aataaaatga aactaattaa
     2221 tatgaaatga ggtgcttatg ccaaccattc gtgatattgc tagaaaagct aatgtttcac
     2281 ccgcaactgt ttctcgtgtc ttaaacggcg acccaactat caatgttcaa gctaaaacaa
     2341 aacgaagaat ttttgaagtt gccgaagact tagcctacac taaacatttg cgaccaaaga
     2401 aaaatcaaca aaaaaataaa ttccgtattg ctatttatca ctggtataca atcgaacaag
     2461 aaattaatga tccttatttt atttcaatta gaattggcgt ccaacgagca tgtcgtgaac
     2521 ttggcattgc ttttgatgta atttatcgtg aagatcgcat cacagatcct tttgaagatc
     2581 agaaatacga tggtgtgatt atgattggta gttttacgag agagacacaa acatttattt
     2641 ctgatcgctt ttcggctgtt gtgtacgcta attcggaaga cgaggatggt aaatgcgatt
     2701 gtgttcgcgt tgatttccgc gcactaacac acgatgttct tcatttacta atcaataaag
     2761 gtcataagaa aattgcttat attggtggcc gtgagcgcgt ttcttctgaa ccaaacaaaa
     2821 cacctgatcc gcgtgagtta gaatatattg aagtaatgca tcaaaacaag ttatattata
     2881 aaccttatgt taaggttggt gaatatagcg ctcatagtgg ttatgaaccc gcaaacgaac
     2941 taattgacaa taatgaaggc aacacaccaa cagcgatatt ctgcggcaat gactcgattg
     3001 ctttaggtgt ttcaaaagct atccaggaac gcggttttaa aataccagac gatattgcta
     3061 tatttggtgt taatgatatt cctaccctgc aatttacttc tccttcatta tcatcagtta
     3121 aaatccacac tgaatttatg ggcgaattag cgcttaaact actaaacgaa cgcatgaaaa
     3181 aagaacgttc actaaaatta agtgtaaacg ttccttatga actcatttta cgcgaaagtg
     3241 cttaaattaa tttatttcat ttgttaaggt atcttctgtt gatgcctttt tttcttcact
     3301 tttaactacc ttcggattag gaataactag gttaagtaat accgctacaa gcgtggcaat
     3361 tgctaaggga gataattcaa catttccaat cattacttta agtaaacgcg ggtcaaaagt
     3421 tgttacacca accgcgctaa ggatatcact tgcgacacta agcgcaccta gtcctaaccc
     3481 aaccgctaaa gtaacactta caacgattaa gtcttttgtt tggccaatat cagctttatg
     3541 ttcaatgacc atccgtaaac ctgaagcact aatcattcca aagagaataa ttgatgcccc
     3601 accaataacc gcacttggaa tagtagcgat tacattacca aaaatgctaa tcacaccaaa
     3661 gataaccgct aaaattgcag cataaaacac attacgcggt tcatagtttt ccgtaattac
     3721 taaaatagca gtattttcac catatgttgt ttgcggtggt ccgcctaaag cactagcggc
     3781 cataatcgca aaaccgtcgc ctgctaagtg cgatcaatac caggatcaac aaaaaagtct
     3841 ttgccgcata ctgtactatt agcgcttatg tctccaatat gttccatcag tgttacaaaa
     3901 gcaattggaa ccatcataag aatagcgttc caatcaaact ttaaatattt ataaaagcct
     3961 agaagcgaac caatactttg aaatatgaca atatcactac ctttgagtgt gctaaagtcg
     4021 actaacccaa ctattgctcc gtatacataa ccaatcaaaa agccaagtaa aattggcgct
     4081 gctttaaaaa atgatttggg cttagcaaaa gcgctaatgg caataatact taagagtgta
     4141 ataagcgcag tcgtccacac cttccacgcc tcagcgccat aaagcgcata tggggcaatg
     4201 atattattcc aaaagattga aggtacaagt aacatcccta acacaataat cacaggaccg
     4261 acgacacgcg gcggaaacat ttttcgcacc ttttcaacac caattaggcg aattactccg
     4321 gcaaaaatga ggtacattac accagtaaag aaaatagcaa ttactagttt gcccatattt
     4381 tgttgccacg caagactccc cacttcgcca ctttccgcca taatactcat cagtgctggc
     4441 aagtaagcga aacttgaacc aagaaatact ggtactttcc tttttgtcac aaaatagaaa
     4501 atgattgtcc caatccctgc ggtaataagg gtaatagaaa ttgatagacc cgctagcatt
     4561 ggcacaagca cagtcgcacc gagcatcgca aacatatgct gaaaacttag gactgttgtt
     4621 ttcataaacc ctttgagacg ggttttctta ttagtttcct tcataataat ttcttccttt
     4681 tagttattaa tcattattct accatttatt catttagtga atctaagtat gttactaacc
     4741 tacttgcgtg actttcatta tattcatcac ttttaataaa tgcttttaat tcttttagtg
     4801 ttaaccattt atgattaatt gtttcttctt tttgatattt aattgtttta tctttcgcgc
     4861 ttataatcgc taaataccct tgattaattg ctggtacatc tttttggata aagtaataag
     4921 ttggtgttac ttcgttaatg actaatcctg attcttcaaa tacttcacgg attgctcctt
     4981 cttcttttgt ttcaccagcc ttaattgaac cgccacagct tccttcatat ttcccgggat
     5041 attctttttt atcataggag cgttgcatca ctaaatatgt accatctgtg tgcttaatta
     5101 atgtttcaac aataatatga taatagcctt caggtattaa tgcgccttta tcaacaattt
     5161 tccctgtttt cttttcatcc tttgtatata aatcccattt ttccatgaca taacaccttt
     5221 aattattcgt aattaaaact attgtatcaa aatatattta ttttcttaaa gactaatcta
     5281 agtgaatcgt gtttcactaa atatttttat aattataaaa tcaaaagaaa gagccgcatt
     5341 tgcggctccc tccatttagc ttattttaga atttattcaa atattatgcc ttcttcataa
     5401 tcactaaagt ctggtaatgt gatagtgaac tcttcattga attttggttt catatcaagg
     5461 aaaccactca atttgccaac ttgtgtatat ccttccggat atgtttcact tggttcataa
     5521 accattgttg taattaattc aaagtccgcg cgcacttgcg ttaagtgatc attaccatct
     5581 gcgccaattg taatcttgta ctctctgaga tcgagcactt cttctaggag cgcaacatac
     5641 cactgcagaa attcttcggc ttctgcccgt tcttcacttg tcatttcttc actaaagaga
     5701 attccgcgta agccgctttc catcatcgca ataaacgcat ctttgtttat gctatagatg
     5761 acagtgatat ttctcccatt tttcg
//
LOCUS       DAOSEX010000015         5727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_12400_length_5727_cov_3.055536, whole genome shotgun sequence.
ACCESSION   DAOSEX010000015 DAOSEX010000000
VERSION     DAOSEX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12400_length_5727_cov_3.055536"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PK612_00625"
     CDS             complement(<1..133)
                     /locus_tag="PK612_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH94654.1"
                     /translation="MQINLDHNKKYLLACSYGPDSMALFSLLLKGNYNFVVAHINYGL
                     "
     gene            complement(123..332)
                     /locus_tag="PK612_00630"
     CDS             complement(123..332)
                     /locus_tag="PK612_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94655.1"
                     /translation="MAKDISTAGKVIVDKRTVKPSYTVKVDDVLELYLGAHIVVVKVL
                     TVDEKEIKSNPEKGYELLSRTKNAN"
     gene            complement(370..3843)
                     /gene="mfd"
                     /locus_tag="PK612_00635"
     CDS             complement(370..3843)
                     /gene="mfd"
                     /locus_tag="PK612_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOH94656.1"
                     /translation="MIKFFETLFPKNTPPRFEKERVFVTSEIIGTTLLVAEAFKKKRE
                     NYVVLTNNLYNAQKIISTLQTFVSQDDILFFPNDDLLFTEVLTATKELEAQRIYTLFS
                     SLEKDKKYIYVANLSALLKPTLSPTSFAKQFFELKVGNTVKMSALTTKLVELGYKNVT
                     NVEQTMQFAKRGDIIDIYTVNEDFPTRIEFFDDEIESIRDFNVSTQVSIKTRDKVTVL
                     PASENLLFPNEQKEFLKCLQEFFNVQKKDMTSAEEEMFATNLNASISSYLEGDNNALN
                     KYLLIYSLFQNSLLSYIDNPKVVFANEPQLLATYEIISNHAEHYVRDQARRFNLLPKT
                     QLYQPYNTLDHYSKYVTREFSEDGNEGELRISPVADLRSRDDFNLVVQKYYDDNYLIY
                     FSVNNNTQLEQVKNLLKELQLDFDLDDKSSDKRILITYDYFPHGFVINSCKTAVFTSK
                     ELFNITRNEMRFNARFKAGTVIRSHEQLTPGDYVVHEYHGIGQFMEIESLKVFGVVGD
                     YIKIKYFGNEELSIPMEQIHLIRKYSGREGVAPRLHKIGSPVWQRTKVEISKKIEDIA
                     EELIAIQAARYQRPGFAFPPDDEFQKEFELGFPYELTYDQEIALREIKNDMESPLIMD
                     RLLSGDVGFGKTEIALRAAFKAISAGKQVMFLCPTTLLARQHYEVSLERFANFGVKIA
                     MISRLVTQKEKDEIVKDFQAGNIHLLIGTHALFNIDVESVDLGLLIVDEEQRFGVEQK
                     EIIKKMKTRVDVLTLSATPIPRTLQMSLVGIRGLSQINTAPQERMPIQTYVIKRDDYV
                     IKELVERELGRGGQVFYLYNRIATIYHVANKLSRLVPQAKIGVVHGKMDRADIEDVMT
                     SFYNNEINVLVATSIIENGIDVPNANVIIVENADTFGLSQLYQIKGRVGRGNRIAYAY
                     LLYQGNKIMNDDAKKRLQAIQEFTDLGSGYKIAQRDLMIRGAGDILGAEQSGFIDSIG
                     IDMYLKMLEDAINEKKYKLTSDPIEKTINISGLEAYIPDDFASKSEKFDIYLEIKKSK
                     KMKELKDFEEKVIDMYGKLPQSLEILFLKRQVDLVVNSRANFIEDIKDEDVFIDIIPG
                     RAFLDIPGSVVMLNNEIGTTYQNIRFITQDDKIKLRIIKRGDWFHAYASIVKAIVMIV
                     RETMKNKDETR"
     gene            complement(3836..4417)
                     /gene="pth"
                     /locus_tag="PK612_00640"
     CDS             complement(3836..4417)
                     /gene="pth"
                     /locus_tag="PK612_00640"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006506439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOH94657.1"
                     /translation="MKLFVGLGNPGKKYENTRHNAGFLALDKFAEIAKVDIDKSDFKG
                     LFTSFTYNAEKIYLLKPQTYMNLSGQSVVSLMNFFKIEVDELYVIYDDMDISPGQIRL
                     KEDGSSGGHKGMQNIIELLGTTNIKRIRIGIGKPEHDGVDHVLQKLTDEDKKIFMEGV
                     TKASVAVRDLLNEDFNTVMNKNNRKEPKPPPSD"
     gene            4464..5393
                     /locus_tag="PK612_00645"
     CDS             4464..5393
                     /locus_tag="PK612_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOH94658.1"
                     /translation="MNRFLKRFLQGLLLGFALITAIGGGTMAILIGVYDELINAVSDF
                     RKDPKKSLSLLGPMALGGIVSMGALFYPIKLALEHFPFLSISFFAGLTLGGLRIFTGT
                     IKGKVTWKNLLLTLAGFLFVFGLGTFTWFTTLHADLVNINFLQVILLFFVAFLSMGGH
                     VSAGISGTFILIAFGYYEELIDFLIRLAKFDFVNVGFDLAGLLSFGIGSILGLIVIAK
                     TYKYFFEKDRVKTYFTIFGFIIGSIVIAFFNGKVRPEYAKVTGFDPLMFTLCVVAVIV
                     GAALSYFLLGLANKKEKQQTVEEVAINSNNKED"
     gene            5418..>5727
                     /locus_tag="PK612_00650"
     CDS             5418..>5727
                     /locus_tag="PK612_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94659.1"
                     /translation="MEWEITFLRWVVENTQTPFITAIFKFLTLSGELAVLYFIIAFIM
                     IGFKKTRKAGLIIVFGLILVSGFNLWIFKPIVNRPRPFDVPKFSADALWLNNYMYNVF
                     A"
BASE COUNT         1669 a         1007 c          904 g         2147 t
ORIGIN      
        1 ttaatccgta attaatatgc gcaactacaa agttatagtt tccttttagc aatagtgaaa
       61 aaagcgccat agaatcagga ccatagctgc acgctaataa gtattttttg ttatgatcaa
      121 gattaatttg cattttttgt tctacttaat aattcatatc ctttttctgg attacttttt
      181 atttcttttt catcaaccgt aagtactttt acgacaacga tatgtgctcc taaatataat
      241 tctaaaacat catcaacttt aacagtgtat gatggtttaa cagttctttt atcaacgata
      301 acttttccgg ccgtacttat atcttttgcc accgttcttc gtttaattaa tcgcgtcaat
      361 tttagaaatt tatctagtct catctttatt tttcattgtt tcacgaacaa tcatgacaat
      421 tgctttaaca attgacgcgt atgcgtgaaa ccaatctcct cttttaatta tccttaactt
      481 aattttatca tcttgggtta taaaacgaat gttctggtat gttgtcccta tttcattatt
      541 taacattact actgagccag ggatatctaa aaatgctctt cctggaataa tatcaataaa
      601 tacatcttca tctttaatgt cttcaataaa gttagcgcga ctatttacca ctagatcaac
      661 ttggcgtttt aaaaataaga tttctagtga ttgcggtaat ttaccataca tatcgattac
      721 tttttcttca aagtctttta gttctttcat ctttttactt tttttaattt cgagataaat
      781 atcaaatttt tctgacttac tagcaaaatc atcaggaata taagcttcta atccgctaat
      841 attgattgtt ttttctattg gatcacttgt tagtttatat tttttctcat ttattgcatc
      901 ttcaagcatc tttaaataca tatcaatacc gatactatca ataaacccag attgttccgc
      961 acctaaaata tcaccggcac cacgaatcat taaatcgcgt tgtgctattt tgtagccgct
     1021 gcctaaatct gtaaactctt gaattgcttg aagtcttttt ttcgcatcat cattcattat
     1081 tttgttgcct tgataaagta aatatgcgta tgcaatgcgg tttcctcttc caacccttcc
     1141 ttttatttga taaagttgcg atagtccaaa agtatcagca ttttcaacga tgattacatt
     1201 tgcatttggc acatcaatcc cgttttcaat aatagaagta gcgactaaaa cattaatttc
     1261 gttattatag aaacttgtca ttacatcttc aatatcagcg cgatccattt tcccatgtac
     1321 aacgccaatt ttagcttgtg gcactagcct gcttaactta tttgcgacat ggtatatcgt
     1381 tgcaatacga ttatataaat aaaatacttg tccgccacgc cctaactcgc gctccactaa
     1441 ttctttgatt acataatcat ctcttttaat gacataagtt tgaattggca ttctttcttg
     1501 gggagcagta ttaatttgac ttaagccgcg gatgccaact aatgacattt gtaatgtacg
     1561 cggaatagga gtggcgctca atgttaaaac atcgactctt gttttcattt ttttaataat
     1621 ctctttttgt tcaacgccaa acctttgttc ttcatcgaca attaataatc caagatcaac
     1681 gctttcaaca tctatattaa acaaagcatg tgtaccaata agcaaatgaa tattgccagc
     1741 ttgaaagtct tttacgattt catccttttc tttttgagta acaagacgag aaatcattgc
     1801 tatttttaca ccgaaattag caaatctctc tagtgacacc tcatagtgct gtcttgcaag
     1861 taacgttgtg ggacataaaa acattacttg cttaccagcg cttattgctt taaaggcggc
     1921 gcgaagtgct atttctgttt taccaaagcc tacatcaccc gaaagaagtc ggtccataat
     1981 aagtggtgat tccatatcgt ttttaatttc ccgaagcgct atttcttggt cgtaggtcaa
     2041 ttcatatggg aaaccaagtt caaattcttt ttgaaattcg tcatcaggcg gaaaagcaaa
     2101 acctggtctt tgataacgcg ctgcttgaat agcaataagt tcttcagcaa tgtcttcaat
     2161 ttttttgctt atttcaacct ttgttctctg ccatactggt gaaccaattt tatgcagtcg
     2221 cggtgcaacg ccttctcggc cactatattt gcgaattaaa tgaatttgtt ccattggaat
     2281 tgataattct tcattgccaa aatatttaat tttaatatag tcaccgacaa caccaaatac
     2341 ttttaaggac tcaatttcca taaattgacc aataccgtga tattcatgaa ctacataatc
     2401 acccggggta agttgttcat gactacgaat cacagttccg gctttaaacc tggcattaaa
     2461 ccgcatctca ttacgcgtaa tattaaatag ctctttacta gtaaatacag ctgttttgca
     2521 ggaattaatg acaaatccat gcggaaaata gtcataggta attaatattc ttttatcgct
     2581 acttttatca tctaaatcaa aatctaattg taattctttt aataaatttt taacttgctc
     2641 tagttgtgtg ttattgttaa ctgagaaata aataagataa ttatcatcat aatatttttg
     2701 cactactaaa ttaaaatcat cacgactgcg aagatccgct accggactta tacggagttc
     2761 accttcattg ccgtcctcgc taaattctcg tgttacatat tttgaatagt ggtctaaagt
     2821 gttataaggt tgataaagtt gtgtctttgg taacaagtta aatcgtcttg cttggtctct
     2881 aacataatgc tctgcgtgat tagaaataat ctcatatgta gcaagtaatt gtggttcatt
     2941 agcgaataca acttttggat tatcaatata agagagcaaa ctattttgaa acaatgaata
     3001 aattaataga tatttattta aagcgttatt atcaccttct aaataactac taatacttgc
     3061 attcaagtta gtagcaaaca tttcttcttc cgcgctagtc atatcttttt tctgtacatt
     3121 gaaaaattct tgcaagcact taagaaattc tttttgttca ttggggaata ataagttttc
     3181 acttgcagga agcactgtta ctttatcacg agttttaatt gatacttgtg tacttacatt
     3241 aaagtcacgg attgattcaa tctcatcatc gaagaattca atacgcgtcg gaaagtcttc
     3301 gtttactgta tatatatcga taatatcacc gcgttttgca aactgcatcg tctgttcgac
     3361 attagtgaca tttttatatc ctaattccac caatttggtg gttaacgctg acattttaac
     3421 agtatttcct acttttagtt cgaaaaattg tttggcgaaa gatgttggag aaagcgtcgg
     3481 tttaagcagc gctgataggt tagcaacata aatatatttt ttatcttttt caagtgatga
     3541 aaataatgtg taaattcttt gtgcttctag ttctttagtt gcggtcaata cttccgtaaa
     3601 taataaatcg tcatttggga aaaataaaat atcgtcttga ctaacaaatg tttgtaaggt
     3661 tgaaattatc ttttgtgcat tatataaatt gttcgttaaa acaacatagt tttctcgctt
     3721 tttcttaaaa gcctcggcga ctaatagcgt tgtccctatg atttcgcttg tgacaaaaac
     3781 tcgctctttt tcaaagcgag ggggtgtatt tttaggaaac aatgtttcaa aaaatttaat
     3841 cactcggtgg gggttttggc tcctttcggt tgtttttatt cattacagta ttgaaatctt
     3901 catttaacaa atcacgaacg gcaacgcttg ctttagtgac gccttccata aaaatctttt
     3961 tatcttcatc agtgagtttt tgtaaaacat gatccactcc gtcgtgttct ggtttaccaa
     4021 tgccaatacg aatccgttta atatttgtgg ttcctaagag ttcaataata ttttgcattc
     4081 ctttgtgtcc gccagatgac ccgtcttcct ttaaacggat ttgtcctgga gaaatatcca
     4141 tatcatcata aattacataa agttcatcaa cttcaatttt aaagaagttc attagtgaca
     4201 ctactgattg tccagaaaga ttcatatatg tttgtggttt taataaatat attttttcag
     4261 cgttgtaagt aaaactcgtg aaaagccctt tgaaatcact tttatcaata tcaaccttag
     4321 cgatttcggc aaatttatca agcgctaaaa acccagcgtt gtggcgcgtg ttttcatatt
     4381 tctttcctgg attacctaaa ccgacaaata gtttcatatt tttactgata aattataaca
     4441 tttattgtgc tattatttat gatatgaata gattcttaaa acgtttttta caaggtttgc
     4501 tcttaggatt tgccttaatt accgcgattg gcggcgggac tatggctatt ttaattgggg
     4561 tttatgatga gttaattaat gctgtttctg attttaggaa agatccaaaa aagtcgctta
     4621 gtttactcgg accaatggcg cttggcggta ttgtaagtat gggcgcgctt ttttatccaa
     4681 tcaaactagc gcttgaacac tttccttttc tttcaatttc attttttgct ggtttaacac
     4741 tcggcggttt acgaatattt actggtacta ttaaaggcaa agtcacttgg aaaaacttac
     4801 ttttaacact tgcgggtttc ctttttgtgt ttggcttagg aacttttact tggtttacga
     4861 ctttacacgc tgatttagta aatattaatt tcttacaagt catcttgctt ttctttgtgg
     4921 cctttctttc aatgggcggt catgtttctg caggtatatc aggtacattt attctcattg
     4981 cttttggata ttatgaagaa ttaattgatt tcttaattag acttgctaaa tttgattttg
     5041 tgaatgttgg ttttgactta gcgggactct tatcgtttgg tatcggttca attttagggt
     5101 taattgttat tgctaagaca tataaatatt tctttgaaaa agaccgcgtt aaaacttatt
     5161 tcacgatctt tggttttatt attggctcaa ttgttattgc tttctttaac ggtaaggtac
     5221 ggccagagta cgctaaagtt actggttttg accccttaat gtttactctt tgtgttgttg
     5281 ctgttattgt aggcgcggcg ctttcttact tccttttagg tttagcaaat aaaaaagaaa
     5341 aacaacaaac agttgaagaa gttgctataa atagtaataa taaagaagat tagaatataa
     5401 aaaaggagta cttaactatg gaatgggaaa ttactttttt acgctgggtt gtagaaaata
     5461 cacaaacacc gtttattacc gctattttta agtttttaac actctctggc gaacttgcag
     5521 ttctctattt tataattgct tttattatga ttggatttaa aaagacgaga aaagcaggat
     5581 taatcattgt ttttggttta attcttgtat ctggttttaa tctttggatt tttaaaccga
     5641 ttgttaatcg gccgcgacct tttgatgttc ctaaattttc tgcggatgct ttatggttaa
     5701 acaactatat gtataacgtc tttgcgc
//
LOCUS       DAOSEX010000016         5603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_12803_length_5603_cov_2.506489, whole genome shotgun sequence.
ACCESSION   DAOSEX010000016 DAOSEX010000000
VERSION     DAOSEX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12803_length_5603_cov_2.506489"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PK612_00655"
     CDS             <1..918
                     /locus_tag="PK612_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH94660.1"
                     /translation="ALFLILVAVEFFLPLRALGSAFHVAMNGASAGQKILSLLAEEET
                     PWGTKEVTNANIEIKDVTFAYNGHANVLNDVSMTFKEQGMVSIVGESGSGKSTIVNLI
                     TGALRPQFGSVSVGGELLETLSRENYYSNLTVISYNTYIFNDTIRNNFKLANKDVTDE
                     EIYAALTKVNLADFVEANGGLDKVINEDALNISGGERQRLAVAINLVTNKAIYIFDEA
                     TNNIDIESEAIIMANIKELSKKKAVIVISHRLANVVPSDYIYYMENGELLESGNHDTL
                     MANNSGYAKLFTTQKALEEGYMECNYEKA"
     gene            905..2599
                     /locus_tag="PK612_00660"
     CDS             905..2599
                     /locus_tag="PK612_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94661.1"
                     /translation="MKRPNVIRRSGAKIMASLVVLLGSLSYIMVLAIITGSLGFVFAM
                     GVTLLGAVAIAKFLGEAIALSYPLIFSLLIGFGVLRGILRYIEQYTNHYIAFRLLAVL
                     RDKIFSALRTLFPSKIESKQKGSIIAMITSDIEILEVFYAHTISPIFIAIVVSISVIL
                     FVGFSSSWLLAVNALISYIVIGILLPLITSKKLKVSGVRYRQNFAAFNSFFLDSIKGI
                     KDIVLNNGGPKREVEVNERSDMLLNETKKLKKDTTRAHATTELFVSIFIFITLVLGAI
                     LVIRDNLTIGRMIIGVAAIFGSFGPVIAISSLPSNLTQTFAAGDRVLDLLNEKPLITK
                     IDNGSDFEFENVTVSNVSFAYAENEEILNDVSVHANKGEIVGIVGPSGCGKSTLLKLL
                     LRFYKKAEGTILYNGIEIENINSDNLLDNVTMVSQDTYLFDDTILENLLIAKRDATLK
                     EVEIACKKASIYDFIVSLPQGFNSRVGAFGDNISAGEKQRIGLARAFLRGSKLILLDE
                     PTSNVDSINEGIILRALKKHKEDRGIILVSHRESTMAIADRVYRFNGEIVKEVEGK"
     gene            2600..3046
                     /locus_tag="PK612_00665"
     CDS             2600..3046
                     /locus_tag="PK612_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOH94662.1"
                     /translation="MKAKKIFWIILCCVSLALGAIGAALPILPTVPFLLLAAFSFAKS
                     SKRLHNWFINTKLYKKNLASYVKGKGMTWPAKIKMMITVTLVMLLGFIPMMIKKVYIP
                     SIILGVVWIFHIIWFIFGIKTYKKNSEDSLSTEVETPSETEEKNIE"
     gene            3165..3887
                     /locus_tag="PK612_00670"
     CDS             3165..3887
                     /locus_tag="PK612_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOH94663.1"
                     /translation="MKVENIYKQKYVLTTNYYDPSNNIGVQGTLDLFQNIAARHAYLL
                     GAGREHLEKINLVWVIARTEIEYVTYPETPLEVEVVTWPRPATRFYFDRYYKIVNPAT
                     NEIIAYSRSRWVLIDIDSRKIVGADRYDYPLDFYYEEEPFNHTFPRPSVSDNVVGNYT
                     VRPSDIDENGHLNNSKYGNIIYDYLDLQKGQKIKSIVINYNNEALQGETLTISRSRDD
                     AKVVISGHKGDTIVFSSEVNIQ"
     gene            3887..4708
                     /locus_tag="PK612_00675"
     CDS             3887..4708
                     /locus_tag="PK612_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOH94664.1"
                     /translation="MLTTLELWIIIIGMAIPFIATALGAAAVFFFRGPISERVNSILL
                     GFAAGVMIAASVWSLIMPSLEFAENSGWGKLAIIPVAVGFALGVLFLWFIDRIVPHMH
                     QRSEDEEGLPTKKLSLDSKLFLAMTIHNFPEGIAVGFAFAVAYTGIVNPGNASISLYS
                     ALALSIGMAIQNIPEGAAVSLPMARRLNNKGKAFGFGVASAIVEPIGAVLAFLFATVF
                     QTLLPWFLSFAAGAMMFVVVEDLIPEANLSGTEHQGTFAVMLGFIIMMILDIVLG"
     gene            4714..4790
                     /locus_tag="PK612_00680"
     tRNA            4714..4790
                     /locus_tag="PK612_00680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4748..4750,aa:Arg,seq:ccg)
     gene            complement(4848..>5603)
                     /locus_tag="PK612_00685"
     CDS             complement(4848..>5603)
                     /locus_tag="PK612_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOH94665.1"
                     /translation="EGHSWTSYLDRHFPPELVNYYTSPFKTEIAYNCDNGNNESYDEF
                     VPVRVGQGYDSTRFGPEIGIAAHLEKAQRSDEFKIIKFAIGGTSLEVFWRSPSSGIPG
                     LCYVFATNYAHRMLTKLKRDGYNPTVSAILYMQGENDSVDGGHLQYEKYLQNLVNDLR
                     NEFKDYAPKAGIYFIDGLINDAEFWTHHEIINTAKRNVAKSDPKNIIVDTLTPQLSFS
                     NEPDFEIDWAHYDSDGTIKLGHLFGEAVTEHIK"
BASE COUNT         1773 a          872 c         1135 g         1823 t
ORIGIN      
        1 gcgttatttt taattttggt cgcagtcgag tttttcttac cgcttcgtgc gcttggctcg
       61 gcgtttcacg ttgcaatgaa cggggcaagt gctggtcaaa agatattatc actgcttgca
      121 gaagaagaaa cgccatgggg tacaaaagaa gtaacgaatg caaatatcga aattaaagat
      181 gtaacttttg cgtataacgg tcacgctaac gtgttaaatg atgtatcaat gacttttaaa
      241 gagcaaggca tggtttcgat tgttggtgaa tcaggaagtg gaaaatcaac aattgttaat
      301 cttattacag gtgcacttag accgcaattt gggagtgtga gtgttggtgg cgaattatta
      361 gaaactctgt cgcgtgaaaa ttattatagt aatcttacgg tcattagtta caatacttat
      421 atttttaatg atacaatccg caataatttt aaattagcaa ataaagatgt aaccgacgaa
      481 gagatatatg cggctttaac aaaagttaat cttgctgatt ttgttgaagc aaatggtggg
      541 ttagataaag ttattaatga agatgcgcta aatatatcgg gaggagaaag gcaacgcctt
      601 gctgtggcaa ttaatttagt cactaacaaa gcgatatata tatttgatga agcaactaac
      661 aatattgata tagaaagcga agccataatt atggctaata ttaaagaatt atcgaaaaag
      721 aaagcggtaa ttgttatttc tcatcgtctc gctaatgtag tgcctagtga ttatatttat
      781 tatatggaaa atggtgaatt attagaaagt ggtaaccatg atactttaat ggcgaataat
      841 agtggctacg ctaaattatt tacgacccaa aaagcattag aagaaggtta catggagtgt
      901 aattatgaaa aggcctaatg taatacgaag aagtggggca aaaattatgg caagtcttgt
      961 cgtactttta ggtagcttat cttatattat ggttttagca attattactg gctcattagg
     1021 atttgttttc gcgatgggtg tcacactttt aggagcagta gcgatagcta agtttttagg
     1081 cgaagcaatt gcactttcat acccactaat tttcagtttg ttaattggat ttggtgtttt
     1141 acgcggaatc cttagataca tagaacaata cacaaatcat tatattgctt ttcggctttt
     1201 agcggtgcta cgcgataaga tatttagtgc tttacgaaca ttatttcctt caaagataga
     1261 gagtaaacaa aaaggatcaa ttattgcgat gataacttcg gatattgaaa tactcgaggt
     1321 gttttatgct catacaatct cccctatttt tattgctata gttgtgtcga ttagtgttat
     1381 tttatttgtt ggtttttctt caagttggtt attagcggta aatgccctaa ttagttatat
     1441 tgtcatcggt attttactac cacttattac atcaaagaaa ttaaaagttt cgggtgttcg
     1501 ttatcgccaa aactttgccg cctttaactc atttttcctt gatagtatta aaggaattaa
     1561 agatattgtt ttaaacaatg gtggaccaaa acgtgaagta gaagttaatg aacgttctga
     1621 tatgttgctt aacgaaacaa aaaagttgaa aaaagatacg actagggcac acgctactac
     1681 tgaactattt gtttccatct ttatctttat tacattagtg ctcggggcga tacttgttat
     1741 tcgtgataat ttaactattg ggcgtatgat tattggcgtt gctgctatct tcggttcatt
     1801 cggtcctgtt atcgctattt cttctttacc aagtaattta acgcaaacat ttgcagcagg
     1861 tgatcgtgta cttgatttat taaatgaaaa accactgatt actaagattg ataacggaag
     1921 tgattttgaa tttgaaaatg taacagttag taatgtgtct tttgcctatg ctgaaaatga
     1981 agaaatactt aatgatgtta gtgtacacgc taataagggt gagatagttg gaattgttgg
     2041 tccttcggga tgcggcaaat caacattact aaaattgtta ttaagatttt acaaaaaagc
     2101 tgaaggtacg attttatata acggtattga aatagaaaat attaacagtg ataacctttt
     2161 agataatgta acaatggtca gccaagacac ttatcttttt gacgacacta ttcttgagaa
     2221 tttattaatc gctaaacgtg atgcaacatt aaaagaggtg gaaattgctt gcaaaaaggc
     2281 atcaatttac gattttattg tttcacttcc gcaaggtttt aattcacgtg taggagcgtt
     2341 tggcgataat atatccgctg gtgaaaaaca gcgtattggt ctagctcgtg cgttcttacg
     2401 tggtagcaaa ttaatattac ttgatgagcc aactagtaat gtagatagta taaacgaagg
     2461 cattatccta agagcattaa aaaaacataa agaagatcgt ggcatcattc ttgtatcaca
     2521 ccgtgagtca acaatggcaa tcgctgatcg tgtctatcgt tttaatggtg aaatagtaaa
     2581 ggaagttgag ggtaaataaa tgaaagcaaa aaagatattt tggattattt tatgttgtgt
     2641 cagtctcgca cttggtgcaa tcggagccgc acttcctatt ttaccgacgg ttccatttct
     2701 tttgctcgcc gcttttagtt ttgccaaaag ttcgaaacgt ttacataatt ggtttattaa
     2761 tacaaaacta tataaaaaga atctagcaag ttatgttaag ggcaaaggca tgacttggcc
     2821 ggcaaaaata aagatgatga ttacagtgac acttgtgatg ttacttggat ttatcccgat
     2881 gatgattaaa aaagtatata ttcctagcat tattttagga gtcgtgtgga tttttcatat
     2941 catttggttt atttttggta ttaaaactta taagaaaaat agcgaagact cgctttcaac
     3001 cgaagtagaa acaccaagcg aaaccgaaga aaaaaacatt gaataaaatt taaaaagtaa
     3061 gtaaaaaata taaataaaat agtataaata gcctttattt tcacacaaat ggtgtaatat
     3121 tataatgttg ccttaatcga atgattaaag gaggaaaagc gacgatgaaa gtagaaaata
     3181 tttataaaca aaaatatgtg ctcaccacta attattatga tccaagtaat aatattggtg
     3241 tacaaggcac acttgattta ttccaaaata ttgccgcccg tcacgcttat ttattaggtg
     3301 ccggtcgtga acacttagaa aaaattaact tagtgtgggt tatagcgcgc acagaaatag
     3361 agtatgttac ttatcctgaa actccgctag aagttgaggt tgtgacttgg ccacgtcctg
     3421 caacaaggtt ttattttgat cgctattata aaattgtaaa cccagcaacg aatgaaataa
     3481 ttgcttactc cagaagtaga tgggttttaa ttgatattga ttcgcgtaaa atagtaggcg
     3541 ctgatcgtta tgattatccg cttgatttct attatgaaga agaacctttc aatcacacct
     3601 tcccgcgacc cagtgttagt gataatgttg taggtaatta caccgtacgc ccaagtgata
     3661 ttgatgaaaa cggacactta aataattcaa aatacggaaa tataatctat gattatcttg
     3721 atttacaaaa aggtcaaaag attaaaagta ttgtaattaa ttataataat gaagctttac
     3781 aaggtgaaac cttaacaatt agtcgcagca gggacgatgc aaaagtcgta atttctgggc
     3841 ataaaggtga tacaattgtg ttcagtagcg aggtcaatat tcagtaatgt taacaacact
     3901 tgaactatgg attattatta tcgggatggc tataccattc attgcgactg cgcttggtgc
     3961 agcagcggta ttctttttcc gcgggcccat aagtgagcga gttaatagta ttttactagg
     4021 atttgctgcg ggtgtaatga ttgctgcttc agtgtggtca ttaataatgc caagtttaga
     4081 atttgctgaa aattcaggct ggggtaaatt agcgattatt ccagttgcgg tcggatttgc
     4141 attaggagta ctctttttat ggtttattga ccgtattgtc cctcatatgc atcaacgtag
     4201 tgaagatgaa gaaggattac caactaaaaa actaagtctt gattcaaaat tatttttagc
     4261 aatgacaatt cataactttc ctgaaggtat tgcggtcggc tttgcttttg ctgtggctta
     4321 tacaggtatt gttaatcctg gaaatgcttc tatttcgctc tatagtgccc tagccctctc
     4381 aattgggatg gcaattcaaa atattcctga aggagcggct gttagtttac cgatggcgcg
     4441 gcgtcttaat aataaaggta aagcattcgg atttggtgtt gctagtgcaa ttgttgaacc
     4501 aattggtgca gttttggcgt ttctatttgc cacggtgttt caaacactct taccgtggtt
     4561 tttaagtttt gccgcaggag cgatgatgtt tgttgttgtg gaagatttaa ttcctgaagc
     4621 aaatttaagt ggaactgagc accaaggcac atttgctgta atgttaggct ttattattat
     4681 gatgatttta gatatagtat tagggtaata aatgcgctcg tagctcaggt ggatagagta
     4741 caaccctccg aaggttgggg tcacacgttc gagtcgtgtc gggcgcgcca taaagaaaga
     4801 gaaaagcacc cagtcgcggg tgcttttttg tttattgtta ttagtaacta tttaatatgt
     4861 tcagtaacag cttcaccaaa taagtgacca agtttaatag taccatcact atcataatgt
     4921 gcccagtcta tttcaaaatc cggttcattg ctaaaagaaa gttgtggggt aagtgtatcg
     4981 acaatgatgt tttttggatc actttttgct acattacgct tagcggtatt aattatttcg
     5041 tgatgtgtcc aaaattcagc atcattaatc aggccgtcaa taaagtaaat gcctgcttta
     5101 ggtgcgtaat ctttaaattc gttccgtaaa tcattaacaa gattttgtaa atacttttcg
     5161 tattgaagat gcccaccatc aactgaatca ttttcacctt gcatataaag aatagcgctg
     5221 acagttggat tatagccatc acgctttaat tttgtaagca tacggtgcgc ataattagtg
     5281 gcaaacacat aacatagtcc agggatccca cttgatggtg agcgccaaaa tacttcaagg
     5341 ctagtgccac caatcgcgaa cttaataatt ttaaattcat cactgcgctg agctttttct
     5401 aagtgcgcag caatcccaat ttcaggacca aaacgtgtag agtcatatcc ttgtccaact
     5461 ctgacaggga caaattcatc atatgattca ttattaccat tatcacagtt ataggcaatt
     5521 tccgttttga aaggggaagt gtagtaattg actaattcag gcgggaaatg ccggtctaaa
     5581 taactagtcc acgaatggcc ttc
//
LOCUS       DAOSEX010000017         5593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_12835_length_5593_cov_3.075479, whole genome shotgun sequence.
ACCESSION   DAOSEX010000017 DAOSEX010000000
VERSION     DAOSEX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12835_length_5593_cov_3.075479"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="PK612_00690"
     CDS             complement(<1..1440)
                     /locus_tag="PK612_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH94666.1"
                     /translation="MKLLKTVKIINWHYFWNQTLNFEQIVFLTGVNASGKSTLIDAMQ
                     VVLLGDTSGRHFNKAAAEKSNRTLRGYLRGELGDSLEGGFHYLRNGRFTSYIVLEFYD
                     DVNDDHFNLGIVFDVFDDGGVDHRFFMLEAPMPENEFIIDNVPMEYKDLNLFFQEHYP
                     NKYKFFDTNRQYQDALKRKLGNLKDKYFSLLKKAVSFSPITDITTFITEYVTEAQGNI
                     DIAPMQENILQYKKLEDEAALMRFKVERLNEIAKNYEVYKGHKEQLTLFEYVLEKATL
                     QNDLNRLATYDNQYLGYQKRLTDIESELADLDVDLAELNKRRVKLIQDSSSNDTYRIT
                     DEINEHKKVAEEKIKSLKFLNMQVRSDLENYVLTFDRLANEVLEKVSAMDEKRLKKDG
                     LYAREELLESAETIIKENHKITSVLNKDINELSETMLNDWRSAQAKFKQHLGAFNLSL
                     DRLINDYQVNEAILKQQELNLRGGGKPYEY"
     gene            complement(1437..2075)
                     /locus_tag="PK612_00695"
     CDS             complement(1437..2075)
                     /locus_tag="PK612_00695"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOH94667.1"
                     /translation="MFEEAFLQLTRADQDAFAKAINELLLNSFIVRDEFDRREKTIKI
                     NPTYRFIERYYDLINAYLSFAGWRVDKDLILGVFSLVNEYNHNRLRFDREVSLVLFVL
                     RLIYEEERKEGGHVGDSVYLTTPLVMKVMHDRGIGFPNKRLSGRLIGRCLKEIAAHNI
                     IARVSGSYDEGNVTFYLLPSITFAVDNEKIVAISEALEKLALRDAGTEGGNV"
     gene            complement(2077..3492)
                     /locus_tag="PK612_00700"
     CDS             complement(2077..3492)
                     /locus_tag="PK612_00700"
                     /inference="COORDINATES: protein motif:HMM:NF039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HOH94668.1"
                     /translation="MSLFTIIPSNFFSILASKNKEIYAEALLLLHHSIQDNELLIKKR
                     DFTRNLRDRLNELALSVDFDAEEEGDEDEIVNHSDKAAFIVRRLEECGWIDVEVNPDN
                     FEEYIALPSYSINVISLLNDLVKESTAEYVSLVHATYSELKLEDDMRDEFMYATLVRA
                     FENTKRLNVELITLAHSIRIFQNRLGKVFSTNNILSDYFDLYKTRVSDRYYHPLKTFD
                     SVAKFKRPILQILQKWLYDDDIRQQLVTQSLMWSRNKDAKAAEADIINKINYIADMYE
                     RVNETIDQIDQKHYEYTKASASKILYLNAADKSIKGHLDYIFKTYAETLSKGESVAPI
                     LNGINQSITFQQHGYITPDSLTLPIVRQLRAESIPMSLTDFDDVRDMLMQGFLDETSS
                     QFSNDAIFEFMDIAFDEESEVHISDIALPSFDAFLLLILATIKTSDEQCFYIIETEEG
                     SVSSYGYRLPNLTFVRKEITL"
     gene            3619..4686
                     /locus_tag="PK612_00705"
     CDS             3619..4686
                     /locus_tag="PK612_00705"
                     /inference="COORDINATES: protein motif:HMM:NF039499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HOH94669.1"
                     /translation="MANYELSRNTVSDLLSDNIQISPNTNEKLDYFRRAIKNAYPDYQ
                     KKFRHRARSFAVFAEIIIKRHNHTIKNNSIKHQKTYFKNDAYIYHIIEDFILAEEAKQ
                     NPEHTFTRDEYVDPTILQFENLIDHRYQNLLRYDFQKIKDPKLTLYNLTSRFFQELVS
                     GIMLLEREFYNDSFIIWRSLLETTTTLLILYENDNLVGKFNERRNIALMRVKVVDASR
                     QTLKSKAKETKQQLGFKGVPDYVAERYGWAGDLFKSRDYSLRTLLERINMVDLYSHYA
                     FASLFVHEYLISPEDLRLEIDFEKYLLSLYFKLYEAVRIKINDFTNDLDEVKKLEQGV
                     RKEVNNFKAQFNDFSTRIQTT"
     gene            4683..5132
                     /locus_tag="PK612_00710"
     CDS             4683..5132
                     /locus_tag="PK612_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94670.1"
                     /translation="MKDKRIYTSFRGLFLIYLPIYLLMSIIFYLIVGIKFPPTLNHYL
                     TVGGWTIITAIYLIFGYKNSYYELTKHEIVHHKGSNILYYKFDDIIYIDKVYSGKKLS
                     IRFFTRIGDERYLAHDPKKKVYNTMLERCTNLIDKEELLRRFPKIKY"
     gene            complement(5122..>5593)
                     /locus_tag="PK612_00715"
     CDS             complement(5122..>5593)
                     /locus_tag="PK612_00715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOH94671.1"
                     /translation="HHNIFEIKKVMDDLGFRIINLVIWQKTDPPPLIYKNKLKFSHEI
                     LIWAKKGRSHFFNYDLAYSLRNSELGDVWKFSAVQNHEKKFGYHPTQKPERLLERIIR
                     LTTNKNDVVLDPFMGSGTTCFVAKKLQRQYIGIELEEEYFSIAKRRIDSIKANT"
BASE COUNT         1838 a          996 c          874 g         1885 t
ORIGIN      
        1 atattcatat ggtttaccac caccgcgtaa atttagttct tgttgtttta aaattgcttc
       61 atttacttga tagtcattaa ttaaacgatc aagacttaag ttaaaagccc caagatgttg
      121 cttaaatttc gcttgagcac tgcgccaatc attaagcatt gtttcactaa gttcattaat
      181 gtctttattt aaaacactag tgattttatg attttcttta ataattgttt cggcgctttc
      241 aagtagttct tcgcgcgcat ataatccgtc tttctttagc cgtttttcat ccatcgcact
      301 aactttttct aaaacttcat tagcgagtct atcaaatgtt aggacataat tttctaagtc
      361 actacggact tgcatattta agaatttaag acttttaatt ttttcttctg cgactttttt
      421 atgttcatta atttcatcag tgatgcgata agtatcatta cttgaactat cttgaattaa
      481 tttaacacgg cgtttattaa gttcagcaag gtcaacatct aaatcagcaa gttcactttc
      541 aatatctgtt aagcgttttt gataacctag atattgatta tcataagttg ctagtcgatt
      601 taagtcattt tgtagtgttg ctttttctaa gacatattca aaaagtgtta attgttcttt
      661 atgacctttg tatacttcat agtttttagc tatttcattt aagcgttcaa ctttaaaacg
      721 catcagcgct gcttcgtctt caagcttttt atattgcaaa atattttctt gcattggtgc
      781 aatatcaata ttgccttgtg cttcagtgac atattcagta ataaaagtcg taatatccgt
      841 aatgggtgag aaactaacag ctttctttaa gagactaaaa tacttatctt ttaaattacc
      901 aagtttccgt ttaagggcat cctgatattg acggttcgtg tcaaagaatt tgtatttatt
      961 tggataatgt tcttggaaaa ataaatttaa atctttatat tccatcggga cattatcgat
     1021 gataaattcg ttttccggca ttggtgcttc taacataaag aagcgatgat caaccccgcc
     1081 gtcatcaaac acatcaaaga caatccctaa gttaaagtga tcatcattaa catcatcata
     1141 aaactctaaa acaatgtaag aagtaaagcg tccgttccgt aaatagtgga atccgccttc
     1201 taacgagtcg ccaagttccc cgcgtaaata tccgcgtaat gtccggtttg atttttccgc
     1261 ggcggcttta ttaaagtggc ggccggaagt atcaccaagg agcacaactt gcattgcgtc
     1321 aataagtgtt gacttcccgc tagcgttcac cccagtcagg aagacgattt gttcaaaatt
     1381 taaggtttga ttccaaaaat agtgccaatt aataattttg actgttttta ataatttcat
     1441 acattacctc cctcggtgcc tgcatcgcgt aaggctaatt tttcgagagc ttcgctaatt
     1501 gcgacaattt tttcattatc aacggcaaaa gtaatcgatg gtaataagta gaaagtaaca
     1561 ttaccctcat cataacttcc cgagactcta gcaataatat tatgtgccgc gatttctttt
     1621 aaacaacgac caattaagcg cccacttagg cgtttatttg gaaaaccaat gccacgatca
     1681 tgcattactt tcatcacaag tggtgttgtt aagtacacag aatcgccaac atgtccccct
     1741 tctttccgtt cttcttcata aattaggcgc aagacaaata acactaagct tacttcacga
     1801 tcaaagcgta agcggttgtg attatattca ttaacgaggc taaatacacc taatattaaa
     1861 tctttatcaa cacgccagcc tgcaaaactt aaataggcat taattaagtc gtaatagcgt
     1921 tcaataaacc gataagtcgg attaatttta attgtttttt cgcgacgatc aaattcatca
     1981 cgcacgataa aactattaag taataattca ttaattgctt tagcaaaggc gtcttgatct
     2041 gctcttgtta attgtaaaaa ggcttcttca aacatattat aaggtaatct cctttcgtac
     2101 gaatgttaag ttcggtaatc ggtaaccata gctactaaca ctaccttcct cggtttcgat
     2161 tatgtaaaaa cattgttcat cactcgtttt aattgttgct aaaattaaaa gtaagaatgc
     2221 gtcaaatgac ggtaaagcaa tatcactaat atgtacttca ctttcttcat caaacgcgat
     2281 atccataaat tcaaagattg catcattaga aaattggcta cttgtctcat caaggaaccc
     2341 ttgcattaac atatcacgca catcatcaaa gtcagtaagt gacatcggaa tactttcagc
     2401 gcgtaattga cgaacaattg gtaaggttaa tgaatcaggg gtaatgtaac catgttgttg
     2461 gaatgtaatt gattgattaa tcccgttaag aatcggcgcg acactttcac ctttacttaa
     2521 tgtttcagca taagtcttaa aaatataatc aaggtgacct ttaatcgatt tatccgcagc
     2581 atttaaatat aaaatctttg aagcactagc tttcgtgtat tcatagtgtt tttgatcaat
     2641 ttggtcaatt gtctcattaa ctctttcata catatcagca atgtagttaa ttttattaat
     2701 aatatcggct tcggccgctt ttgcgtcttt gttgcggctc cacattaatg actgtgtcac
     2761 tagttgttga cggatatcat cgtcatacaa ccacttttgt aaaatttgta aaattggtcg
     2821 cttaaattta gcgacactat caaatgtttt aagcgggtga taataacggt cactcacgcg
     2881 cgttttatat aagtcaaaat aatcacttaa aatgttatta gtgctaaata cttttcctaa
     2941 gcgattttgg aaaatgcgga tgctgtgcgc taaggtgata agttcgacat taaggcgttt
     3001 tgtattttca aatgcccgca ctaatgtcgc atacataaat tcatcgcgca tatcatcttc
     3061 taacttaagt tcgctataag tcgcgtgcac aagcgaaacg tattcagcag tgctttcttt
     3121 cactaaatcg tttaataagg aaataacatt aatactataa cttggaagcg caatgtattc
     3181 ttcaaaatta tcaggattaa cttcaacatc aatccagccg cattcttcta aacgacgaac
     3241 gataaatgcc gctttatcgc tatgattaac gatttcgtct tcatcgcctt cttcttccgc
     3301 gtcaaaatca acactaagcg ctagttcatt taaacggtca cgcaaattac gagtaaaatc
     3361 gcgcttcttt attaataatt cattgtcttg aattgaatgg tgtagtaaaa gaagtgcttc
     3421 agcatatatc tctttattct tactcgcaag aatcgagaag aagttactag gtatgattgt
     3481 aaatagactc aaaaaaaata atctccttcg gttatatgtt ttatattaac atataaaaaa
     3541 atgaattggt attaaacttg tataataaaa gagaaatatt taatttcgtt taaaataata
     3601 atagaaaggc ggtaacatat ggcaaattat gaacttagtc gcaatactgt tagtgacctt
     3661 ttaagtgata atattcaaat ttcaccaaac acgaatgaga aacttgatta cttccgtaga
     3721 gcaattaaaa atgcttatcc tgattaccaa aaaaagttta gacatcgtgc tcgttcattt
     3781 gcagtatttg cggaaatcat tatcaaaaga cataatcata cgattaaaaa taacagcatt
     3841 aaacatcaaa aaacatattt taaaaatgat gcttatattt accatatcat tgaagatttt
     3901 atcctagcgg aggaggctaa acaaaatcct gaacacactt ttacacgtga tgaatatgtt
     3961 gacccgacaa tcttacaatt tgaaaactta attgatcatc gttatcaaaa tttattaaga
     4021 tatgattttc aaaaaataaa agatccaaaa ctaactcttt acaacttaac aagccggttc
     4081 tttcaagaat tagtgtcagg aataatgctc ttagaacgcg aattttataa tgactccttc
     4141 atcatttggc gcagtctctt agaaacaaca acgaccttat taatacttta cgaaaacgat
     4201 aatctagttg gtaaatttaa tgagagacgc aacattgcgc tgatgcgtgt taaggtcgta
     4261 gatgcgagtc ggcaaacact aaaaagcaaa gcaaaagaaa ctaaacaaca actaggcttt
     4321 aaaggtgtgc cagattatgt tgcagaacgt tacggttggg cgggtgattt atttaaatcg
     4381 cgcgactatt ccttacggac actacttgaa agaattaaca tggttgacct ttattcgcat
     4441 tatgcctttg cctcgttatt cgtccacgaa tatttaataa gtccagaaga tttacggctc
     4501 gaaatagatt ttgaaaaata tttattatca ctttacttta aactttatga agccgtgcgg
     4561 attaaaataa atgacttcac aaatgacctt gatgaagtta aaaaattaga acaaggagtg
     4621 cggaaagaag ttaataattt taaagcacaa tttaatgact ttagtacgcg aattcaaaca
     4681 acatgaaaga caaacgaatt tacacaagtt ttagaggatt atttttaatt tatttaccaa
     4741 tatatttatt aatgagtatc atcttctatc tcatagttgg tattaagttt ccaccaacct
     4801 taaatcacta tctaacagtg ggtggctgga caattattac tgcaatatat ttaatatttg
     4861 gttataaaaa tagttattat gaactaacaa aacatgaaat agtgcaccat aaaggtagca
     4921 atatccttta ttataaattt gatgacatca tttatattga taaagtgtat tccgggaaga
     4981 aacttagtat ccgcttcttt acgcgaattg gcgatgagcg ctatttagcg catgatccaa
     5041 agaaaaaagt gtataacacg atgcttgaac gctgcactaa tcttatcgat aaagaggaat
     5101 tattacgcag attcccaaaa attaagtatt agcttttata ctatcaatcc ttctttttgc
     5161 aattgaaaaa tattcttctt ctaattcaat accgatatat tgacgctgaa gttttttagc
     5221 gacaaaacaa gtagtgccac tacccataaa tggatctagt accacatcat ttttgtttgt
     5281 ggttaatcga atgattcttt ctaataatct ttcaggcttt tgtgtgggat gataaccaaa
     5341 ttttttctca tgattttgta ctgccgaaaa tttccaaaca tcgccaagtt cactattacg
     5401 caaactatag gctaggtcat aattaaaaaa gtgtgacctt ccttttttag cccaaattaa
     5461 tatttcatgc gaaaacttta atttgttttt ataaattaat ggtggtgggt ctgttttttg
     5521 ccagattact aaattaatta tccgaaaacc aagatcatcc atcacctttt taatttcaaa
     5581 aatattatga tga
//
LOCUS       DAOSEX010000018         5550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_12980_length_5550_cov_4.173430, whole genome shotgun sequence.
ACCESSION   DAOSEX010000018 DAOSEX010000000
VERSION     DAOSEX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12980_length_5550_cov_4.173430"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PK612_00720"
     CDS             <1..267
                     /locus_tag="PK612_00720"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOH94672.1"
                     /translation="VLIAVDPKKNELLVPVEIYSRGFINWSISNLLPDAEKHINKVLK
                     RHYAEKVTTFGEVKNIVKDELEPFFYDKTGRKPMIIPVIMSKGI"
     gene            269..811
                     /locus_tag="PK612_00725"
     CDS             269..811
                     /locus_tag="PK612_00725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013631739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOH94673.1"
                     /translation="MFILASKSPRRQELFKLIIDDFAIIVPDIDENIPVSIKSDTAKI
                     ISKFKAYHIKAKYPEATILACDTIVVIDGELLGKPKDEDDARRMLTKLSGRKHHVISG
                     FTLVSPEFEINKNVVTEVYFNTLSPELIEAYIKSGSPFDKAGAYGIQDEEFKLVREIK
                     GSYYNVMGLPVEELRKFIKK"
     gene            complement(788..1144)
                     /locus_tag="PK612_00730"
     CDS             complement(788..1144)
                     /locus_tag="PK612_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HOH94674.1"
                     /translation="MTFSEFLQQYGVYLAFVVFLAIVFLIILILVIPRLPKKGTKSVE
                     PSISKLEIITLLGGEDNIKEIALRGSRLTVSLADKSLANIEELKKHGVDRVIVMQAKL
                     VLLVNKEVASLFDELA"
     gene            complement(1134..1940)
                     /locus_tag="PK612_00735"
     CDS             complement(1134..1940)
                     /locus_tag="PK612_00735"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003424.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOH94675.1"
                     /translation="MALKINVIVREDEKSLNVSKSLCELALQKGFLIDEVNPDFVFSV
                     GGDGTFLKAVHRYLTKVKHIKFVGIHTGSLGFFADFRPEDINNLLDNIIKEELNTNAY
                     RLVKAEITQNNKKRNFYAVNEVRIENVHHTLVLDVYLNDEHLEMYRGNGVLVATQLGS
                     TGYNKSIGGAVINRNLELLEYTKIAPIANSAFRPLVNPLIIGEKDVLVFNGKNANTYF
                     GYDAFTKKLSDIPFSVKVSLSNKKITIVHRQNRPYSRILNESFLGGKHDI"
     gene            1996..2178
                     /locus_tag="PK612_00740"
     CDS             1996..2178
                     /locus_tag="PK612_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94676.1"
                     /translation="MATSIEIEAKALLTKKDYNKVLKHFRLDVVDGYIQKNYYIDTEN
                     KDLRKLGLSPVFRVIH"
     gene            2368..4068
                     /locus_tag="PK612_00745"
     CDS             2368..4068
                     /locus_tag="PK612_00745"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOH94677.1"
                     /translation="MAYFLKQTKQGDRIYLQISETTYNNVTKKSSNRVYQKLGYLDAL
                     ISEEMPDPIAFYKAKIVKMNKKLEQQKAEESILKIAEDPTRNIGYFLVKALVNTLKLS
                     DEMELMNRVHDYNFNMAAFVEALTYSRIVEPCSKLKTYTDIFPYLYEKYDLSLDQIYS
                     GLDFVGSRYSQFIEILNYRINKTFSRDTKYTYFDCTNYYFEIDLEKDDKQKGPSKENR
                     HDPIIGQALLLDGEGIPLGMKMYAGNKSEKPMLKKLIDEIKRTHNLSGKTVHVADKGL
                     NSGENIHQTLKDGNGYIYSQSVMQLDEKERKWVLSPYDYEDVKDNSGNLLYRIKACVD
                     NFPIWINTEDGKKERVLLKQKRVVTFNPSLAEKKRIEINKQIDKAINLSHSQAKKEEY
                     GDSSKYVKFVDSDGNKADIIINEDKIKKDLELAGYNLLVTSELKMSKREVYRVYHNLW
                     KIEDTFRVLKSELDARPVYLQNKERIYGHFLICYYGVTLLRLLETKIFKDTFNIYDII
                     NLIRNLKILKGKDKNTNLSKATKNINTLTQKYDFNIDNFYLTDNDVDKLFKYKYRLKK
                     "
     gene            complement(4128..>5550)
                     /locus_tag="PK612_00750"
     CDS             complement(4128..>5550)
                     /locus_tag="PK612_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94678.1"
                     /translation="ISKRDEKIRLAWAQVNPLNELFDANMTPMLVEKTIPLLTMDPFF
                     DSKRLDYLVRKYELSDFTSDNESAEFVQSGEIQGNPFILARTLVMNMGTKTYTGTLTV
                     SYTVTVYVNGKAQRQTRTETLRASVNKPCPYYNNDTYIIYGNEAAPNLIFSRYPQLPL
                     DWTEKSLRRFVEKEEKEMKKLSEKALKQGKQFTPLGNTEFEALFNAYDRNHELEYRLL
                     FTALGQSEILDLIKDKKVGFGDNFQFFKNKNLNLVRSKHSQAFDYSGNPRNYYHYDNK
                     EIRKRFVDYNNNYLHRFYFNIAPVLAIPLYQQHKPHEFIYQTEYKSHLSFYEHESTVN
                     QFPSSAFAHPDSHTQNILKTKVVQKGKDFDTVSVTSYGFTTVERVDYVPKMAGNGTIH
                     NVPVHWTEYIPVEKESHATIKVLDEESVKKTQNESFFDKIGEYLSKYTPDQKPVTGRH
                     VMAFLVASKFADDDNEGLDKLFK"
BASE COUNT         1941 a          887 c          942 g         1780 t
ORIGIN      
        1 gtgcttattg ctgttgatcc aaagaaaaat gaacttttag tgcctgttga aatttatagt
       61 cgcggcttta ttaattggag tatttctaat ttattaccag atgccgaaaa gcatattaac
      121 aaagtattaa agcgccacta tgctgaaaaa gtaacaactt ttggtgaagt taaaaacatc
      181 gttaaggatg agctcgaacc attcttttat gacaaaactg gtcgtaaacc aatgattatt
      241 cccgtcatca tgagtaaagg aatttagtat gttcatccta gcgtcaaagt caccgcgccg
      301 ccaagaatta tttaaattaa ttatcgatga ttttgcaatt atcgtccccg atattgatga
      361 aaatattccg gttagcatta aaagcgacac cgctaaaata atttctaaat ttaaggctta
      421 tcatattaag gcaaagtacc ctgaagctac tattttagcg tgcgatacaa ttgtggtcat
      481 tgacggtgaa ctacttggta aaccaaaaga cgaagatgac gctagaagga tgctcactaa
      541 attaagtggg cgcaaacacc atgttatcag tggcttcaca ctcgttagtc ccgaatttga
      601 aataaacaaa aatgttgtta ctgaagtata tttcaacact ttaagtcctg agcttattga
      661 agcatatata aaaagcggct caccatttga taaagcggga gcctatggta ttcaagacga
      721 agaatttaag cttgtccgtg aaattaaagg tagttactat aatgttatgg ggctaccggt
      781 agaagaatta cgcaagttca tcaaaaagtg atgcgacttc tttattcacg agtaaaacaa
      841 gttttgcttg cataacaatt acacggtcga caccgtgttt ttttaattct tcaatgtttg
      901 caagcgactt atccgcaagt gatactgtta aacgcgaacc gcgtaacgct atttctttaa
      961 tattatcttc accacctaaa agtgtaataa tttctaattt tgaaatgctt ggttcaacac
     1021 tttttgtacc ctttttaggt aaacgcggaa taactaaaat taatatgatt aaaaagacaa
     1081 ttgctaaaaa tacaacaaaa gctaaataca caccatattg ctgcaaaaac tcactaaatg
     1141 tcatgtttac ctcctaaaaa agattcattt aatatacggg aataaggacg gttttgacga
     1201 tgaactattg ttattttttt attactaaga ctcactttaa cactaaaagg tatgtcactt
     1261 aatttcttgg taaaagcgtc ataaccaaaa tatgtattgg catttttccc gttgaacact
     1321 aacacatctt tttcaccaat aataagcggg ttaacaagtg gccgaaatgc actgtttgca
     1381 atcggtgcta tttttgtgta ttcaagtaat tctaaattac gattaatcac agcgccgcca
     1441 attgatttat tgtaacccgt actgccaagc tgcgtagcaa ctaacacccc attaccacga
     1501 tacatttcta gatgttcatc atttaaataa acatcaagca caagtgtgtg atgaacattt
     1561 tcaatccgca cttcgttgac cgcataaaag ttacgcttct tattattttg ggtaatttct
     1621 gcttttacta agcgataagc attagtgttt agttcttctt taataatgtt atctaaaaga
     1681 ttgttaatat cttccggacg gaaatcagca aagaatccaa gtgaaccagt atgaatgcca
     1741 acaaacttaa tatgtttaac tttagtgaga tagcgatgga ctgcttttaa aaatgttccg
     1801 tcacccccaa cactaaagac aaagtcagga ttaacttcat caatcaaaaa gcctttttgt
     1861 agtgcaagtt cacataaact tttacttaca ttaagactct tttcatcttc acgcacaatt
     1921 acattaattt taagggccat aactttataa atcttcgttt atattgtatc atattgatag
     1981 aaagaggtaa ttactatggc cacgagtatt gaaattgaag caaaagcact actgacgaag
     2041 aaagattata acaaagtatt aaaacacttt aggctagatg ttgttgatgg ttatattcag
     2101 aaaaattatt atattgatac tgaaaataaa gatttgcgca aactaggatt aagtcccgtc
     2161 tttagggtta tacactagaa aagtttaggg gataaaaata ataattaaag agttatctta
     2221 ctatgtaaga tactttttta taatggggtc ttgacaaaat ttatttttgt tatcatttgt
     2281 ctagtgtaat aaagtgtaat atgatataat ggtaggtagt ataaaatatt actgaataaa
     2341 cttactataa agagagaact taaatgtatg gcgtacttct taaaacaaac taagcaaggg
     2401 gatagaattt atcttcaaat ttcagaaact acttacaaca atgttacaaa gaaaagtagc
     2461 aatcgcgttt atcaaaaatt aggttattta gacgctttaa tcagtgaaga gatgcctgac
     2521 cctattgcat tctataaagc aaagatagtg aaaatgaata aaaagttaga gcaacaaaag
     2581 gctgaagaaa gcattctaaa aattgcggaa gatccaacac ggaatattgg ttatttctta
     2641 gttaaagcat tagtgaatac cttaaagcta agtgacgaaa tggaattaat gaatagagtg
     2701 catgattata attttaatat ggcggcattc gtcgaagctc ttacttattc acgaatcgtt
     2761 gaaccatgca gcaaactaaa aacatatact gatattttcc cgtacctcta tgaaaaatat
     2821 gatttatcgt tagaccaaat ctatagtggg ttagactttg tgggttcaag atattcacag
     2881 tttattgaaa tccttaatta ccggattaat aaaacattta gtcgtgatac gaaatataca
     2941 tattttgatt gtacgaatta ttattttgaa attgatttag aaaaagacga taaacaaaaa
     3001 gggccctcca aagaaaatcg tcatgatccg attattggtc aagcacttct tttagatggt
     3061 gaaggaattc ctttaggcat gaagatgtat gccgggaata aaagtgaaaa accaatgctt
     3121 aaaaaattaa ttgatgaaat aaaaagaacc cataacctca gtggaaaaac agttcatgtc
     3181 gcagataaag gcttaaatag tggtgaaaac atccatcaaa cattaaaaga tgggaacggt
     3241 tatatttatt ctcagtccgt catgcagctt gatgaaaaag aacgtaaatg ggttctttct
     3301 ccatatgact atgaagacgt caaagacaat agtggaaatc ttctttatcg cataaaagca
     3361 tgtgtcgata actttccaat ttggataaat actgaagacg gtaaaaaaga aagagtatta
     3421 ttaaaacaaa aaagagtcgt aacttttaat ccttctcttg cggaaaagaa gagaattgaa
     3481 attaataaac aaattgataa agctatcaat ctttctcact cacaagcgaa gaaagaagaa
     3541 tatggtgact cttcaaaata cgttaaattc gtggatagtg atggtaataa agcggacatt
     3601 attattaatg aagacaagat taaaaaagac ctcgaactcg caggttataa cttactagtc
     3661 acaagtgagc ttaaaatgtc aaagcgtgaa gtatatcgtg tttatcataa tttatggaag
     3721 attgaagata catttagagt actaaaaagt gagttagatg ctagaccagt atatcttcaa
     3781 aacaaggaaa gaatatatgg tcacttctta atttgttatt atggtgttac gttactaaga
     3841 ttactagaaa caaagatatt taaagataca tttaatattt atgacatcat taatttaatt
     3901 agaaacctta aaatcttaaa aggaaaagat aagaatacta acctttcaaa agcaacaaaa
     3961 aatatcaata ctttaaccca aaaatatgac tttaatattg ataatttcta tctcacagat
     4021 aatgatgttg ataaactctt taagtataaa tatcgcctca aaaagtagtt ttacactaga
     4081 aaattggctt aagtccaaaa gtcaggtatt attaataaat aaacttatta cttaaataat
     4141 ttgtcaaggc cttcattatc gtcatcagcg aatttacttg ccacaaggaa agccataaca
     4201 tggcggccgg tgaccggttt ttgatcaggg gtgtatttac ttaaatattc accaatctta
     4261 tcaaagaatg attcattttg tgttttcttc acgctctctt catcaagtac tttaatcgtt
     4321 gcgtgtgatt ctttttcaac tgggatatat tcagtccaat ggacagggac attatgaata
     4381 gtgccatttc ctgccatttt gggaacatag tcaacgcgtt ctacagtagt aaaaccgtag
     4441 ctagtcacac tgacggtatc gaagtcttta cctttttgca caacttttgt ttttaagatg
     4501 ttttgtgtat gactatcagg gtgcgcgaaa gcactacttg ggaactggtt caccgttgat
     4561 tcatgttcat aaaatgaaag gtgcgattta tattctgttt gataaataaa ttcatgtggt
     4621 ttatgttgtt gataaagcgg gattgcaagg acaggagcga tattaaaata gaaacgatgc
     4681 aaataattat tgttataatc gacaaagcgt tttctaattt ctttattatc atagtgataa
     4741 taattacgtg gattcccact ataatcaaag gcttgtgaat gtttagaacg aacaagattt
     4801 aagtttttgt ttttaaagaa ttggaagtta tcaccaaacc caactttctt atccttaatt
     4861 aaatctaata tttcactttg acctaaagca gtaaataata agcgatattc taattcatga
     4921 ttgcggtcat aagcgttaaa gagtgcttca aattcagtat taccaagtgg tgtaaattgt
     4981 ttaccttgtt ttagtgcttt ttcactaagt tttttcatct ctttttcttc tttttcgaca
     5041 aagcgtcgta aactcttttc tgtccaatca agtgggagtt gcggataacg actaaagatt
     5101 aaattaggcg ctgcttcgtt accataaata atataagtat cattgttgta gtatggacac
     5161 ggtttattca cacttgcgcg caatgtttct gtgcgtgttt gtcgttgcgc tttaccgtta
     5221 acatagacgg taaccgtata agaaacagta agtgtcccag tataagtttt agtacccata
     5281 ttcatcacaa gcgttcttgc taaaataaat ggattacctt gaatttcacc cgattgcaca
     5341 aattccgcgg attcattatc agaagtaaag tcacttaatt cgtatttacg cactaagtaa
     5401 tcaagccgct tggaatcaaa aaatggatcc attgtaagca gcggaattgt tttttccact
     5461 aacattggtg tcatattcgc gtcaaacagt tcattaagcg gattaacttg tgcccatgct
     5521 aggcgaattt tttcgtcacg cttactaatt
//
LOCUS       DAOSEX010000019         5515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_13073_length_5515_cov_3.343407, whole genome shotgun sequence.
ACCESSION   DAOSEX010000019 DAOSEX010000000
VERSION     DAOSEX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13073_length_5515_cov_3.343407"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PK612_00755"
     CDS             <1..646
                     /locus_tag="PK612_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH94679.1"
                     /translation="IYGKNKFKETLNVSGFYNTKGILEKILPFTNEEAFRNTKIEGYV
                     TLAKVPTKGLFAKLNMKNYEKEVVFLNESEHPLKAVLASAAIPLVFGEKEIEGTRYVD
                     GGLVDNFPFTPALIANANIIISIGLDPKLLPTKPLPGRLHIDFTPLEELGPFPKVSLD
                     FSPEKTQQYFDLGYENAKALIAYLKREKFFWFGGRLKLRKGRLVTLKTLTAKTK"
     gene            690..2420
                     /locus_tag="PK612_00760"
     CDS             690..2420
                     /locus_tag="PK612_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94680.1"
                     /translation="MKKGLFKYFKVVWKEGILAIFFILFETVLETLVPFMASRVVDVG
                     IANNDLPYIYKWGALMVCSAFLAFFIGIISVRYVARTGQGFGAELRRAQFAKLQSLDQ
                     EARDKFAVSSLITRLTNDTYTIQNAIIIGMRGIFRAPFMITMVLMLSFNMNSKVAIVF
                     ALTLPVIAFAMLVIVFRVRPLYERMQRNFDELNRTLQENFQAIRVVKAYVTEEQEEAK
                     FKAINKMYRKVASNAFMTVSLNSPIMQLGLYSTIIGLLLVGGNLINTGEAKIGEIQGL
                     LTFVLQLLNAMLMLAQILIMFGRTSASKSRISEVLNTDTSLEYLINDLSVKAKPSLIV
                     KDVSFKYPGTSDDNYALKNINFTLKAGETLGIIGQTGSGKSTLVHLLARLYDVTDGEI
                     ILDDHNVKDYSADNLLDAINIVFQNNMLFSGTIRDNLLWGNKDASDEEINRALEASLS
                     DEIVNKLANGLNSEVGQAGSLLSGGQKQRIALARALLKRPKILVLDDSLSAIDTINEG
                     KIRTKLRKLYPELTLIIVAQRVSAIKDADHILVLHEGEIHGAGTHNSLVENDEIYHDI
                     YTTQLKGAKA"
     gene            2417..4264
                     /locus_tag="PK612_00765"
     CDS             2417..4264
                     /locus_tag="PK612_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94681.1"
                     /translation="MKKQSKPKHMKQTMRRLFSYLLKRPVPLVFIVLLVVWSGFTNGY
                     GIYVMREIIDKSILDFDINGLNKSLIKLAILFGSGALSALTYNPLMVKYSQTVIADIR
                     NELFAHSQKLPLSYFDRTPRGEIMNYFTNDVDTLNQALNTGFTTMIYSVVTGIGTIVF
                     LFLINFYLSLIVVFFIVVMFFMVLRNSQKMRVGFASVQKDTAHLNSYIEEMMLGLKVS
                     AMFSHQKEDMKEFKRRNDALTKSGIYANTLVLRKTPMIVSLSYFNYAISAIAGALFVL
                     NGWLSLGALGSYLIYVRQSSNPFNNFMTELNALYNGLSGAERIFAFLDLEQEIDEGKV
                     RLVSAVEIDGNIVETEHPTNSLAWKIPTTNGEFTYEIFKGRVDFTNVVFGYKQDERVL
                     NNISLHAEKGEKVALVGSTGAGKTTIINLITRFYEIDSGTINYDGIDIRDIKKSDLRQ
                     SVSVVIQDTHLFTGTIFENIAYPSPGATIAEVERAAKIAHAHDFIVKLPQGYETMLYD
                     NGANLSSGQRQLIAIARAAILQPPVLILDEATSSIDTRSERLIEKAMDELMEDKTVFV
                     IAHRLSTIRNAKAILVMEKGSIIERGSHEDLILEGKVYYQLYTGQFEWT"
     gene            4419..>5515
                     /locus_tag="PK612_00770"
     CDS             4419..>5515
                     /locus_tag="PK612_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94682.1"
                     /translation="MKRKKAKKHSILHLLKGHFWGMFFVTLLVIFHRVSYSFVPLFTQ
                     YILKYLQNNGVVTGVNLPLFVIEIFEKGQNVFEVSLYVALTLLLYQLFRFSLMVVEQR
                     LKGQVQESIALKLRVKLFDHIQSLSYHYHNNSDTGDLIQRVTTDVEVTTNFVVMRVMD
                     AVGLIFMVLSGAYQMYFLSPLLMYISLAVIPLYGISSYFYFKNIWKRYNDIEEKEAEI
                     LTVIQENVHNAKVVKAFANEKFETEKLEVKNKAHKNAVIKTNKIVGMYWAAMDFFALS
                     QYFVITLISVFLARNGQMDAGNAAAALMLVGLLIWPIRGLGRLINDVSHANVAYNRIT
                     TVLDEKSEFEINGDLLPEIAGKIEFKNVSFK"
BASE COUNT         1770 a          834 c         1094 g         1817 t
ORIGIN      
        1 gatttatggt aaaaacaaat tcaaagaaac attgaatgta agcggctttt ataacactaa
       61 aggtatctta gaaaaaatat taccgttcac taatgaagaa gcatttagaa atacaaagat
      121 tgaaggctat gtcactttag caaaagtacc cactaaaggt ttatttgcaa aattaaatat
      181 gaaaaattac gaaaaagaag ttgtcttttt aaatgagagt gaacacccgc taaaagctgt
      241 tttagcaagt gctgctattc cgcttgtctt tggggaaaaa gaaattgaag gcactcgtta
      301 tgtggatggc ggtttagtcg ataatttccc gtttacacca gcgcttatcg ctaacgctaa
      361 tataattatt tcaattggtc tagatccaaa acttttacca acaaaaccat tacctggtcg
      421 tttacatatt gattttaccc cgctagaaga gttaggaccg tttcctaaag tgtcgcttga
      481 ttttagtcct gaaaaaacac agcaatattt tgatttaggg tatgagaatg ctaaggcgtt
      541 gatagcgtac ttaaaacgcg aaaagttttt ctggtttggt ggaagactaa agttaagaaa
      601 aggacgccta gtaacgttaa aaacgttaac agcaaaaact aagtagctta tatatagaga
      661 aaaagagatt ttactggcat aataatgcta tgaaaaaagg attattcaaa tattttaaag
      721 tagtatggaa agaagggatt cttgccatct tttttatttt atttgaaact gttttagaaa
      781 cactagtgcc atttatggca agtcgcgttg ttgatgttgg tatcgctaat aatgatttac
      841 cttatatcta taaatggggt gcccttatgg tatgttcggc ttttcttgct ttctttattg
      901 gcatcattag tgtccgttat gtcgctcgta ctggtcaagg atttggggcg gaattacgtc
      961 gtgctcaatt tgcgaaacta caaagtttag accaagaagc gcgcgataaa ttcgcagtgt
     1021 cttccttaat tactcggctt acaaatgata cttacactat tcaaaatgcc atcattattg
     1081 ggatgcgcgg gatattccgt gctccattta tgattacgat ggtattaatg cttagtttca
     1141 atatgaattc taaagtagca attgtatttg cacttacgct tcctgttatt gcgtttgcga
     1201 tgctagtcat tgtttttcga gttagaccac tttatgaacg gatgcaacgt aattttgatg
     1261 aattaaaccg tactttacaa gaaaactttc aagcgatccg tgttgttaaa gcttatgtta
     1321 cggaagagca agaagaagca aagtttaagg cgattaataa gatgtatcgt aaagtcgcta
     1381 gtaatgcgtt tatgacagta tcactcaata gtccgattat gcaacttgga ctttattcaa
     1441 cgattattgg tcttctgctt gtcggtggta atttaattaa tactggcgaa gctaaaattg
     1501 gtgaaattca agggttacta acctttgttt tacaactctt aaatgcgatg ctgatgcttg
     1561 cgcaaatatt aattatgttt ggtcgcacta gcgcttcaaa aagtagaatt agtgaagtac
     1621 ttaacactga cactagtctt gaatacttga ttaatgattt aagtgttaaa gcaaaaccga
     1681 gtttaattgt gaaagatgta agttttaagt atccgggcac aagtgatgat aattatgctt
     1741 taaaaaacat taatttcacg cttaaggctg gtgaaacact tgggattatt ggtcaaactg
     1801 gaagcggtaa atcaacactc gtccatcttt tagcacggct ttatgatgta actgatggtg
     1861 agattatttt agacgaccat aatgttaaag attatagtgc ggataatctt ctagatgcta
     1921 ttaacattgt ctttcaaaat aatatgctct ttagcggaac aattcgtgat aacttgctat
     1981 ggggtaataa agatgcaagt gacgaagaaa ttaatcgggc attagaagca agtctaagtg
     2041 acgaaattgt gaataaatta gcaaatggtt taaatagtga agtcgggcaa gcaggctctt
     2101 tgctttcagg tggtcaaaaa caacgaatag cattagcaag agcattacta aaaagaccaa
     2161 aaatattagt gcttgatgat tctttatcag cgattgatac gattaatgaa gggaaaattc
     2221 gcactaaatt acggaaactt tatccggaac ttactttaat tattgttgcg caacgtgtga
     2281 gtgctattaa agacgctgat catattttag tgttgcatga aggggaaatt cacggggcag
     2341 gtacgcataa ctcacttgtg gaaaatgatg agatttatca cgatatttac acgacccaac
     2401 taaaaggagc aaaagcatga aaaagcaaag taagcctaaa catatgaagc aaacaatgcg
     2461 ccggctcttt agttatttat taaaacgacc tgtgccgcta gtttttattg tactccttgt
     2521 tgtgtggagc gggtttacta atggttacgg tatttatgta atgcgtgaaa tcatcgataa
     2581 aagcatatta gactttgata taaatggttt aaataagtca cttattaaat tagcgatcct
     2641 tttcggttca ggtgctttaa gtgcacttac ttataacccg ttgatggtta aatattcaca
     2701 aactgttatc gccgacattc gcaatgaact tttcgctcat agtcaaaaat taccgctatc
     2761 ttattttgac aggacaccgc gcggtgaaat tatgaactat ttcactaatg atgttgacac
     2821 acttaatcaa gcacttaata ctgggtttac aacgatgatt tattcagtag taacaggcat
     2881 tggcacaatt gtatttcttt tccttattaa tttttatttg tcactgattg ttgtcttttt
     2941 catcgttgta atgtttttta tggtactgcg taatagtcaa aaaatgcggg ttggttttgc
     3001 tagtgttcaa aaagatactg cgcatttaaa tagttatatt gaagaaatga tgttagggct
     3061 taaagtatca gcaatgtttt cacaccaaaa agaagacatg aaagaattta agcggcgcaa
     3121 tgatgcatta acgaaaagcg gaatatacgc gaatacttta gtgttacgaa agacaccaat
     3181 gattgttagt ttgtcgtatt ttaattatgc gattagtgcg attgctggtg cgctttttgt
     3241 attaaatggc tggctaagtt taggagcact aggatcatac ttaatttatg tccgccaaag
     3301 ttcaaatccg tttaataact ttatgactga attaaatgct ctctataacg ggctttcagg
     3361 ggcagaacgg atttttgctt ttcttgactt agaacaagaa atagatgaag gtaaagtccg
     3421 gcttgtcagc gctgttgaaa ttgatggtaa tatcgttgaa actgaacacc cgactaatag
     3481 tttagcgtgg aagattccga cgactaacgg ggaattcacc tatgaaatat ttaaagggcg
     3541 agtcgatttt actaatgttg tttttggtta taagcaagat gaacgcgtct taaataatat
     3601 tagtttacat gccgaaaaag gtgaaaaagt tgcgcttgtt ggttcaactg gtgccggtaa
     3661 aacaacaatt atcaatctta ttactcgctt ttatgaaatt gatagcggca cgattaatta
     3721 tgatgggatt gatattcgcg atattaaaaa aagtgattta cgccaatcag taagtgtcgt
     3781 tattcaagat acacatttat ttactggtac tatttttgaa aatattgctt atcctagtcc
     3841 tggcgcaaca attgccgaag tagaacgcgc cgctaaaatt gcgcacgctc atgattttat
     3901 tgttaaatta ccacaaggtt atgaaacgat gctttacgat aacggggcaa atctttcaag
     3961 tggacagcgg caacttattg ctattgcgcg cgctgctatt ttacaaccac cagtcttaat
     4021 ccttgatgaa gctactagct caattgatac ccgtagtgaa agactcattg aaaaagcgat
     4081 ggatgagtta atggaagata aaacggtatt tgttattgct caccgtttga gcactattcg
     4141 aaacgctaaa gcaatattag taatggaaaa aggctcaatt attgaacgcg gaagtcatga
     4201 ggatttaatt ttagaaggta aagtttacta tcagttatac actggtcaat ttgaatggac
     4261 atagaataaa cttccttatg aaagcacttt actaggtaca atttgtgtaa agtatgtaaa
     4321 tatgtacctt ttttaatttt atcgttgtta taaattattc catttaatgt ataatagtag
     4381 agattgtttt aatagaatct attaaaagaa ggatagatat gaagagaaaa aaagcaaaga
     4441 aacattcaat cttacactta ctaaaaggcc acttttgggg gatgtttttt gttacgcttt
     4501 tagttatctt ccatcgtgta tcgtattcct ttgtaccact atttacacag tacattttaa
     4561 aatacttaca aaataatgga gtagtgaccg gtgttaattt accgttattt gttattgaaa
     4621 tatttgaaaa gggtcaaaat gtttttgagg tttcacttta tgttgccctt acgctccttt
     4681 tatatcaatt gtttcgtttt agtttaatgg ttgttgagca acgacttaaa ggccaagtgc
     4741 aagagagcat tgctttaaaa ttacgcgtta aactttttga tcatattcag tcacttagct
     4801 atcattatca taataatagt gacactggtg acctcattca aagagtaacg actgatgtgg
     4861 aagtgacaac aaactttgtc gtaatgcgcg taatggatgc ggtcggttta atattcatgg
     4921 tgttaagcgg ggcgtatcaa atgtattttt taagtccgct tttaatgtat atttcgctgg
     4981 cggtcattcc gctttatggt atttcttctt atttctattt taaaaatatt tggaagcgtt
     5041 ataacgatat tgaagaaaaa gaagcggaaa ttttaaccgt tattcaagaa aatgtccata
     5101 acgctaaagt tgtgaaggct tttgctaatg aaaagtttga aacagaaaag ttagaagtta
     5161 aaaataaagc acataaaaat gcagtgatta aaactaataa aattgttggt atgtattggg
     5221 cagcaatgga tttctttgct cttagtcaat attttgtaat taccctcatt agtgtgtttc
     5281 ttgctagaaa cggacaaatg gatgctggta acgcagccgc agcgttaatg cttgttggcc
     5341 ttttaatctg gccaattcgt ggtttaggcc gattaattaa tgatgtctcg cacgctaatg
     5401 tagcatataa ccgtattacg acagtactcg atgaaaagag tgaatttgaa attaatggtg
     5461 atttattacc ggagattgcg ggaaaaattg aatttaaaaa tgtgtccttt aagtt
//
LOCUS       DAOSEX010000020         5493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_13138_length_5493_cov_3.305075, whole genome shotgun sequence.
ACCESSION   DAOSEX010000020 DAOSEX010000000
VERSION     DAOSEX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13138_length_5493_cov_3.305075"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1092)
                     /locus_tag="PK612_00775"
     CDS             complement(106..1092)
                     /locus_tag="PK612_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94683.1"
                     /translation="MTIYNYLKDYQPILFQTFGNALTTNNLSHAYLLDGEPGTPLKEV
                     AFYLAKSLVCDDPNPFACDKCLACHRFEEGNYTDFYFFDGSESSIKKDSVLELEKAFS
                     MTSVEHKGVLIYVIHNVENMTIEAINSLLKFLEEPAGKVYAFLTINNLHNVIPTIKSR
                     SQILSLQLIKRDVLLKLCESIALPQDDIEMLVPFYNIPSLIEEEAKSERYPEYKRAVI
                     DTLETFSDNPKRGYLKAHELIVPLRERFVIKRVFTYFALFFKDVVNYNIGHERLFKSY
                     DPLIKKLAATIKNPLPLIVATYEISKKVDDYVNTGLLVDELLIKMVKETTDE"
     gene            complement(1089..1712)
                     /gene="tmk"
                     /locus_tag="PK612_00780"
     CDS             complement(1089..1712)
                     /gene="tmk"
                     /locus_tag="PK612_00780"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOH94684.1"
                     /translation="MFITFEGPEGSGKTSIANRIIKTLIDKGYDIVHTREPGGTPIAE
                     QIRDVTLDKKNTALDARAEALLIAASRRQHVVEKIRPALAEGKIVICDRYLDSSLAYQ
                     GAARGLGIDTVLAINLFAVEDTLPNLTLLFDLEPAIGFERIKANKNREVNRLDLEERN
                     FYIKVRNAYLELSKKYKDRYRIIDASQSFDKVYEDTLKIILEYLPQK"
     gene            complement(1722..2321)
                     /gene="recR"
                     /locus_tag="PK612_00785"
     CDS             complement(1722..2321)
                     /gene="recR"
                     /locus_tag="PK612_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOH94685.1"
                     /translation="MDDLPTLNALIEHLKRLPSVGQKSAERMAQAFLTFDDETLKDFG
                     TVITKLKEKVHLCPNCGLYTEAELCSFCVDPNRNNKTLIVVSYPKDVLGFSKLEDYHG
                     RFHILGGVISPSSNKGVELLSIPELEKRIVRDNVEEIILATNPTVEGELTALFVSKKL
                     ADLPVKITRLAYGLPMGGQVDYADAMTLFKALEGRKDLK"
     gene            complement(2321..2626)
                     /locus_tag="PK612_00790"
     CDS             complement(2321..2626)
                     /locus_tag="PK612_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOH94686.1"
                     /translation="MSSSMQKLMQQAQKMKREMQKAQEALNAKEFSVTKGGGVTVAML
                     GSKEVVEVTIDEDLFDKDNKEMVETLIIMAFNECIEQINAEEAAINEAITGSATGLF"
     gene            complement(2619..4394)
                     /gene="dnaX"
                     /locus_tag="PK612_00795"
     CDS             complement(2619..4394)
                     /gene="dnaX"
                     /locus_tag="PK612_00795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOH94687.1"
                     /translation="MSYKALYRTYRPLTFGEIAGQRAIVRTLQNALNQNKIAHAYLFS
                     GPRGTGKTTMAKLFAKALNCEEGLGHQCNRCTNCNSVNDGSHPDVYEIDAASNNGVED
                     VRQLIENINYAPIRGRYKVYIIDEVHMMTQSAFNALLKTLEEPPQNVIFILATTEPHK
                     VIPTILSRCQRYNFSKVSDRDITKRLKTVLETEVIPYEEEALKLLVSLADGGVRDALS
                     MLDQVLAYSRQQLKLEDVLALFALTSKLEQLNLLRAVASSNVEEVFTILSGLIDKGVD
                     VRRLTTDLLNLLKDALIYYRTENTDLLNYLRKEEVQELNMMFEIENLNMMIENLLKAQ
                     ADYRFASDVKNIFEITLLKIITSLNQDTMVEKELIIQKPKVKTAPSVPKKDEKHAIVT
                     ETEKETIVEEQELPPFMTQSREKLEKRVTETKSEIVKDEVKTAQPVPEKVQYANPLHN
                     EGQVIELDDDTIIKILTLADRDEKLSLRNNRWDDLALMAMDSEVGEHANVLLNGAPYA
                     LAPEIILVEFNFDQYARYINILENQKIMQDIIERLVGRRIPVYGLTHGQAVSFARKYR
                     SMSQIQKLPRVKSVKEELEGKFDYV"
     gene            4674..>5493
                     /locus_tag="PK612_00800"
     CDS             4674..>5493
                     /locus_tag="PK612_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH94688.1"
                     /translation="MFNFQLSSNKPQINYDELYDFIALGLGPAGLNAGLYAHRKGLKT
                     LIVGHRVGGQLLNTADIDNYLGFSNIPAQKLIDEFRNHIENSKVPMLTDVLVTNIEKS
                     ADIFIITLNNGKTLQSKTVLYALGGSPRKLKVPGEDRLAGKGVAYCATCDGPFFQDKD
                     VIIAGGGNSAIDATKDLARIAKSVTIIEKFTISADKTSVEQVAKLPNVTMFEETDVTE
                     IIGDKYIEGIRVFDRKANKERIINTEGLFVEIGSIPNTALLKDLVELNKWGEVIV"
BASE COUNT         1705 a         1003 c          894 g         1891 t
ORIGIN      
        1 tactaaaaca taatcgccta tatctaaaga acttcatttg ttccaaaaca ataagacttg
       61 ccaccatcgt tatatataat acttataaag taacgttcat tgttatcatt catctgttgt
      121 ttcctttacc attttgatta ataattcatc cacgagtaag ccagtattaa cataatcatc
      181 aaccttttta cttatttcat aagtagcaac aattaatggc agtggattct ttatcgtcgc
      241 tgctaacttt ttaataagcg gatcataact cttaaaaagt cgctcgtgac ctatattata
      301 gtttacaaca tctttgaaga aaagggcaaa atacgtaaat acacgcttaa ttacaaagcg
      361 ttcacgcagc ggaacgatta attcgtgggc ctttaagtag ccgcgcttag gattgtcgct
      421 aaaagtctct aaagtatcaa ttacagctct tttatactct ggatatcgtt ctgattttgc
      481 ttcttcttca attaaactag gaatattata aaaaggcaca agcatttcta tatcatcttg
      541 cggtaacgct attgattcgc aaagttttaa gagtacatcg cgtttaatta gttgtaatga
      601 aagtatttga cttcgactct taatagtcgg gataacatta tgtaagttat ttatagttaa
      661 aaaagcatat acttttcccg ctggctcttc gagaaacttt aaaagtgaat taattgcttc
      721 aatagtcata ttttcaacat tatgaatcac ataaattaaa acacctttat gttcaacact
      781 agtcatgctg aaggcttttt caagttctaa tacactatct tttttaatac ttgattctga
      841 gccgtcaaaa aaatagaaat cagtgtaatt accttcttca aaacgatgac acgctaagca
      901 tttatcacac gcgaaaggat taggatcatc acacactaaa ctttttgcta aataaaaagc
      961 aacttcttta agtggtgttc caggttcacc atcaagcaaa taagcgtgac ttaaattatt
     1021 tgtagttaaa gcatttccaa atgtttggaa taaaataggc tgataatctt ttaaataatt
     1081 ataaattgtc atttttgtgg gagatattct aaaataattt ttagtgtatc ctcataaact
     1141 ttgtcaaaac tttggcttgc gtcaattatt cgataacggt ctttatattt tttacttagt
     1201 tcaaggtagg cattgcgcac cttaatgtaa aaattgcgtt cttctaagtc taggcgatta
     1261 acttcacgat ttttattagc tttaattcgt tcgaaaccaa ttgctggctc taaatcaaat
     1321 aaaagtgtca aatttggcaa ggtgtcttca actgcaaaaa ggttaattgc caggactgtg
     1381 tcgattccta aacccctagc cgctccttga taggcgagcg aagaatctaa ataacggtca
     1441 caaataacaa tttttccctc ggctagtgct ggacgaattt tctcaacgac atgttgcctt
     1501 ctacttgctg caattagaag tgcttctgcc cgagcatcaa gcgccgtgtt tttcttgtct
     1561 aaagttacat cacgaatttg ttcggcaata ggagtgccac caggttcacg tgtatgaaca
     1621 atatcataac ctttatcaat aagtgttttt ataattctat tagcaatact agtttttccg
     1681 cttccttctg gtccttcaaa tgtaataaac ataacaccct cctatttaag atcttttcgt
     1741 ccttctaatg ctttaaataa agtcatagca tcagcgtaat ctacttgccc gcccattggc
     1801 agtccataag ccaatcgtgt aatttttaca ggtaaatccg caagtttttt actaacaaag
     1861 agtgctgtta gttcaccttc tactgtcgga tttgtggcaa gaataatttc ttcaacattg
     1921 tcacgtacaa ttcttttttc taactctggg attgaaagaa gttcaacgcc tttatttgaa
     1981 gaaggactaa taacgcctcc taatatatgg aagcgcccat ggtagtcttc taatttacta
     2041 aaacccaaaa catccttagg ataactaacg acgattaaag ttttattgtt acgatttggg
     2101 tcaacgcaaa aagaacataa ttccgcttct gtgtaaagac cacaattagg acacaggtgc
     2161 accttttctt ttaatttagt gataactgtg ccgaaatctt ttaatgtctc atcatcaaat
     2221 gttaaaaaag cttgtgccat acgctcagca cttttttgac caacacttgg taaacgtttc
     2281 aagtgttcaa ttaatgcgtt tagtgttggt aaatcatcca ttaaaatagt cctgttgcac
     2341 ttcctgtgat tgcttcatta atagcggctt cttctgcatt tatttgttca atacattcgt
     2401 taaaagccat aatgataagc gtttcaacca tttctttatt atctttatca aaaagatctt
     2461 cgtcaattgt cacttcgaca acttctttac ttccaagcat agcaacagtt acacccccac
     2521 ctttagtaac ggagaactcc tttgcgttta atgcttcttg tgctttttgc atttcgcgct
     2581 tcattttttg cgcttgttgc attaattttt gcatactact agacataatc aaatttccct
     2641 tctaattctt cttttacgct cttaacacgc ggtaattttt gaatttggct catcgagcga
     2701 tattttcttg caaaactcac agcttgacca tgggtaagcc catagactgg tatgcgacgc
     2761 cccactaacc tttctataat atcttgcata attttttgat tttctaaaat attaatataa
     2821 cgagcatact gatcaaaatt aaattcgacg agaatgattt ctggggctag ggcataagga
     2881 gcaccattaa gcaatacatt agcgtgttca ccaacttcac tatccatagc catcagcgct
     2941 aaatcatccc aacgattatt tctaagtgac aacttttcat cgcggtcagc tagtgtaagt
     3001 attttgataa tagtgtcatc gtctaattca ataacctgac cctcattatg taaaggattt
     3061 gcatattgga ccttttcagg cacaggctgg gcagttttca cttcgtcctt aactatttct
     3121 gatttagttt cggtgacgcg cttttctagt ttttcccttg actgtgtcat aaaaggtggt
     3181 aattcttgct cttcaactat agtttctttc tcagtttcag taactatagc gtgtttttca
     3241 tcctttttcg gcacagatgg ggcagttttt acttttggtt tctgaataat taactctttt
     3301 tcgaccattg tgtcttgatt taatgatgtg ataattttta aaagtgtaat ttcaaagata
     3361 tttttgacat cacttgcaaa ccgataatct gcttgagcct tcaataagtt ttcaatcatc
     3421 atgtttaagt tttcaatctc aaacatcata tttaattctt gcacttcttc tttgcgcaag
     3481 taatttaata aatcagtatt ttctgtccga taataaataa gtgcatcttt taataaatta
     3541 agtaaatctg ttgttaaacg tcttacatca acacctttat caattaaacc tgatagtatt
     3601 gtaaaaactt cttcgacatt actactagca actgctctta ataagttaag ttgttctagt
     3661 ttactagtta gggcgaaaag cgctaataca tcttcaagtt ttaattgttg acgagagtaa
     3721 gctaaaactt ggtctaacat gcttaaagca tcacggacgc ctccgtctgc caacgacact
     3781 aataatttta gtgcttcttc ttcataagga ataacttcag tttctaaaac tgtttttaaa
     3841 cgtttagtaa tgtcgcgatc acttactttt gagaagttat agcgttgaca gcgacttaaa
     3901 atcgttggaa ttaccttgtg tggctcagtt gttgccagaa taaaaataac attttgtggt
     3961 ggctcttcaa gtgttttaag caaagcattg aatgcacttt gtgtcatcat atgaacttcg
     4021 tcaatgatat atactttata acgacctcta atcggcgcat aatttatatt ttcaataagt
     4081 tgacgaacat cctcaacgcc attattactg gcggcgtcaa tttcatatac atcaggatgg
     4141 ctgccatcgt taacactatt acaattagtg caacgattac attgatgacc aagaccctct
     4201 tcacaattaa gggctttagc aaagagttta gccatcgttg ttttaccagt accgcgcggt
     4261 ccactaaaaa gataagcatg tgcaatctta ttttgattta gggcattttg taaagtgcgg
     4321 acaattgctc tttgtccagc tatttcgcca aaagtaagtg gccgatatgt tcgatatagt
     4381 gctttataac tcatgtagta attatacaat aattaagact actttgtagg aataagaaca
     4441 caaaacttaa attgttttga taatatgcag gatgatgact ttttattgct tcataatgtg
     4501 tttcattctg caattagttt aatgtttctt tatattaata attgttataa attttattgt
     4561 ctaaaaacat aaaaacacgc attttatgta gcgacacttt ttgcattaag caaattgttt
     4621 ttatttaagt taatcgacct atataatatc ggcagaaagg agaaaactat attatgttca
     4681 atttccaact aagctcaaat aaacctcaaa ttaattatga tgaactctat gattttattg
     4741 ctcttggatt aggtccagct ggtctaaatg ctggattata cgctcatcgc aagggcttaa
     4801 aaactctaat agttggtcat cgtgttggcg gtcaattatt aaatactgct gatattgata
     4861 attatctagg tttctcaaat attcccgcac aaaaattaat tgatgaattt agaaatcata
     4921 ttgaaaattc aaaagtacca atgctcactg atgtgttagt tactaacatt gaaaaaagtg
     4981 ctgatatatt cattattaca ctcaataacg gcaagacttt acaaagtaaa accgtgttat
     5041 atgctcttgg gggtagtccg cgcaagctaa aagtgcctgg tgaagataga cttgctggta
     5101 aaggagtcgc ttactgcgct acgtgtgacg gaccattctt tcaagataag gatgtaataa
     5161 tcgcaggtgg gggtaatagt gcgatagacg ccacgaaaga cttagcgcgt attgcaaaaa
     5221 gtgtgacaat tattgaaaaa ttcacgataa gcgctgataa aactagtgta gaacaagtgg
     5281 caaaattacc aaatgtaacg atgtttgaag aaactgatgt tacagaaatc attggtgata
     5341 aatatattga aggaatcaga gtgttcgaca gaaaggcaaa taaagaaaga attattaaca
     5401 ctgaaggctt atttgtcgaa attgggagta ttccaaatac cgctctatta aaagaccttg
     5461 ttgaattaaa caagtggggc gaagtaattg tta
//
LOCUS       DAOSEX010000021         5457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_13245_length_5457_cov_3.314143, whole genome shotgun sequence.
ACCESSION   DAOSEX010000021 DAOSEX010000000
VERSION     DAOSEX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13245_length_5457_cov_3.314143"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1894
                     /locus_tag="PK612_00805"
     CDS             188..1894
                     /locus_tag="PK612_00805"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOH94689.1"
                     /translation="MAYFLKKTHLKRGLYLQIYESFRDPKKKETAHRSVKALGYVSDL
                     VTSEIPDPVLFYQAEVKRMNEAEKKARAKLLREEISDNSNKNVGHFLLNGFFNRFDLK
                     QEFKYLTFNETFKFDVYELFKALVFARVIEPCSKHKTNIDVIPCLFGNYKFSEHELYS
                     GLAFLGENHENIVEILNYRYEKVYRRNTDKVFFDSTNFYFEIDYEDALRRKGPSKENR
                     RDPLLGMGLLLDGDGIPLAVKFYPGNESERPVVRAVIREMKARHNLKGKVIQVADKAL
                     NTTKNILEALENGDGYLYSQSVKMLSGVETKWVLNENDYKIVHDENGHELYRFKTWVD
                     DFPYFFTKENGQKVKVLLRQKRMVTYNPKLAKKQRGEIIKLAEKAESLTLSGAKKSEY
                     GETGKYVTFTSVDHDGVVGEEKAIISQLNEEKIKRDLSLAGFNMLITSETNLSAKVMY
                     TTYHHLWRIEESFRLLKNKLQARPVYVQTENAIFGHFLINYVALLILRVLQIKVFDDK
                     VHPHQIIDFCKNFNVTKYKYDYFNLGSKRVIKPFSDMLDLDLLPRILKAASIKKLFNY
                     KI"
     gene            complement(1923..2126)
                     /locus_tag="PK612_00810"
     CDS             complement(1923..2126)
                     /locus_tag="PK612_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94690.1"
                     /translation="MALSLSVVTGCKDGSENGGSDDPINFWAYQPSKQTDQTAYKNLI
                     ADFTAETGIKVKLLMVVKDSYNT"
     gene            complement(2255..3208)
                     /locus_tag="PK612_00815"
     CDS             complement(2255..3208)
                     /locus_tag="PK612_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH94691.1"
                     /translation="MATLKDIAKATGFSVITVSRVINSPHLVKEKTRKIIEAEIARQN
                     FKPNVLARALVQNRTNIIYVYIPADIDAINPFFLHVVSGIGEVLGEKGFSLLLKRSWY
                     EGEDCDGCIFMGLSAEDEAKVFELSKTKPVCVFGYVEGVSSVDVDNYQGLYLMTSHVL
                     SKGYRTLTYLGIDDYRRFILQRKKGFSDAVMSQKDKITVKYLAAKNNENDAYKVVGQS
                     LKQGDTPEVIVCATDDMAIGAIRAIKEKGLEVPRDIAVTGFDGLGTQEVAHPRLTTIH
                     QPIYEAGKALAHRIIALIENKNELPVNHAFIETTIIHRESI"
     gene            complement(3263..4885)
                     /locus_tag="PK612_00820"
     CDS             complement(3263..4885)
                     /locus_tag="PK612_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94692.1"
                     /translation="MRKSTFIRRALILVVPALLLTSCVTFGDILKSSEFGATSSSANE
                     VTSEVTGSENPYPLNGTYGVNDYEHPITANPDTLDNYYKVEPLNNNSRLINNYAISNT
                     KGLAHLDSVSNQKMMVVPVKFTDTPASYGGEATIQGAINRAFFGESSDTGWESVASYY
                     YKSSYGRLSLTGEVMPLFDMGYSTTELLAKTVDGYQYWDKSHYVLEQVYNTYLSSTLK
                     EYDQNNDGFVDSVFFVYLAPVDYESDDTLFWAFQYFWNRFADKNKPVFNAYGWASYEF
                     MVEAPGYTYLKPAAHTYIHESGHILSFDDYYDYDGTIAPAGAIDMMDHNIIDHNMFSK
                     FSANWSTPYVPVGNSDIRLRPAESSGDFIIINDAWNGHAYDEYILIEYYTPTGLNARD
                     SLSVSGYNGVKGYTIPGVRIWHVDARLVILSNNGNTKKWSDTIPPHLDGSGGSWMDFA
                     TSNTPSYNYAHNSLHKLHLLDGRGRAQTWANNLVKADDSALFQTGTVIASNNWQKYLL
                     KRATFNDGSVIGYSIEIGEMNSEGVTIKIRKA"
     gene            complement(4887..>5457)
                     /locus_tag="PK612_00825"
     CDS             complement(4887..>5457)
                     /locus_tag="PK612_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94693.1"
                     /translation="YALKRSTTIKLRPFSETGDLLVLKNNWNEKANDEYILLEYYTPT
                     VLNELDSTLNASFKLPKTNGVKVYHVDARTVYEIKNGSIVKYPYVNDYEGEFNGTELL
                     AHSNSTGSYNNAPIKTNKLYKLLEPKESTNISESFNQANQHSLFQKGDEFGYEYFKNF
                     AFNDKTPLNFRFKIVQLTNAYATIEIEIY"
BASE COUNT         1736 a         1030 c          951 g         1740 t
ORIGIN      
        1 tagtaaactc cataataaat acccccatct ttttgattta gcaccatatt ctttttgacc
       61 gaaaaacgat gtttaatact tcaccttact atgtaaggtg atttttatta ttatccttgc
      121 attgacaatg ttttgcaatg tataataatg gtgttaatgg tgttaagcat taacgggagg
      181 aatctttatg gcttatttct taaaaaagac gcatttaaaa cgcgggcttt atttgcaaat
      241 ttatgagagt tttcgtgatc caaagaaaaa agaaacagcg catcgttcgg taaaagcact
      301 tggctatgtt agtgacttag taacgagtga aataccggat ccagttcttt tttatcaagc
      361 ggaagtaaaa cggatgaacg aagccgagaa aaaagcacga gcgaagttac tgcgtgaaga
      421 aattagtgat aacagcaata aaaacgttgg tcacttttta ttaaacggat tttttaatcg
      481 cttcgatctt aagcaagagt ttaaatattt aacatttaat gaaacattta aatttgatgt
      541 ttatgaacta ttcaaagcgt tagtgttcgc ccgtgtgatt gagccgtgtt caaagcataa
      601 aacaaacatc gatgtcattc cttgtttatt tggcaactat aagtttagtg aacacgagct
      661 ttacagcggg cttgcttttc ttggtgaaaa tcacgaaaac atcgtcgaaa tattaaatta
      721 tcggtatgaa aaagtgtatc gccgaaatac cgataaagtg ttttttgaca gcacgaattt
      781 ttattttgaa attgattatg aagatgcgtt acgaagaaaa gggccaagca aagaaaaccg
      841 gcgagatcca cttttaggaa tgggacttct ccttgatggt gatgggattc ctctcgcggt
      901 aaaattttat ccaggtaacg aaagcgaacg accagtggtt cgcgcggtaa ttcgcgagat
      961 gaaagcacgg cataatttaa aaggaaaagt cattcaagtc gcggataaag cacttaatac
     1021 gacgaaaaac atcttagaag cattagaaaa cggggacggt tatctttatt cacagtcggt
     1081 aaaaatgctg agcggtgttg aaacaaaatg ggttttaaat gaaaatgatt ataagatagt
     1141 gcatgacgaa aatggccatg aattatatcg ctttaaaacg tgggttgatg attttccgta
     1201 ctttttcaca aaagaaaacg gccaaaaagt aaaggtgtta ttacgacaaa aacggatggt
     1261 aacttacaat ccgaagttgg ccaaaaagca gcgcggtgaa attataaagc ttgcggaaaa
     1321 agcggagagt ttaacattaa gtggagcgaa gaaaagtgag tacggtgaaa cggggaaata
     1381 tgtaacattt accagtgtcg atcatgatgg tgttgtgggt gaagaaaaag caatcattag
     1441 tcagcttaac gaagaaaaaa ttaagcgtga cctaagtctt gccggattta acatgcttat
     1501 aacgagtgaa acaaacttaa gtgcgaaagt gatgtacacg acttaccatc acttatggcg
     1561 aatcgaagaa agtttccggc ttttaaaaaa taaactccaa gcgcgacctg tctatgttca
     1621 aacagaaaac gcaattttcg gtcacttcct cattaattat gtcgcccttt taatattacg
     1681 agtattacaa attaaagtgt ttgatgacaa agttcatccc catcaaatta ttgatttttg
     1741 taaaaacttc aatgtgacaa aatacaaata tgattatttt aatcttggtt caaaaagagt
     1801 catcaaacca ttcagcgata tgcttgatct cgacttatta ccacgtattt taaaagctgc
     1861 ttctatcaaa aaactcttca attacaagat ttaacaccat tatttacggt gatatcaaaa
     1921 agtcaggtat tataactatc tttaacgacc atcagtaatt taactttaat tcctgtttcc
     1981 gctgtaaagt cagcaattaa atttttatac gctgtttggt ctgtttgttt tgaaggctga
     2041 taggcccaaa agttaattgg gtcatcactc ccaccatttt cactaccatc tttacagcca
     2101 gtcacaacac tgagtgataa agccaaaaaa agactatata agattgttgt tattttcttc
     2161 ggtttccttt tgtgcacctc tataaaatgt tatcgttatc attttaaaat ataattgcta
     2221 attttgcaag ttaaactgaa attctatgta atagctagat tgattcgcga tgaataatcg
     2281 ttgtttcaat gaatgcgtga ttaactggta attcattttt attttcaatc agggcaataa
     2341 tgcgatgggc aagtgctttt ccggcttcgt aaattggttg atgaatggta gtaagtcgag
     2401 ggtgcgctac ttcttgggta cctaacccat caaacccagt tactgctatg tcacgcggta
     2461 cttctaaacc tttttcttta attgcgcgaa tagcaccaat tgccatatca tctgtagcgc
     2521 acacaattac ttcaggagta tcaccttgtt ttaaactttg acccacgact ttataagcgt
     2581 cattttcatt atttttagcg gccaaatatt ttacagtaat tttatctttt tgactcataa
     2641 ctgcgtcact aaatcctttt ttacgctgta agataaaccg tctatagtca tcaattccta
     2701 aatatgtcag tgttcgatag cctttactaa gtacgtgact tgtcattaag taaagccctt
     2761 gataattatc aacgtcaaca cttgacactc cttctacata gccaaagacg cacactggtt
     2821 tagtttttga aagttcaaat acctttgctt catcttccgc gctaagtccc atgaaaatac
     2881 agccatcaca atcttcgccc tcataccacg atctttttaa gagtaaggaa aaaccttttt
     2941 cgccaagtac ttcgcctata ccagacacaa catgtaaaaa gaacgggtta atcgcatcaa
     3001 tatcagcggg aatatacaca taaatgatat tcgtgcgatt ttggacgagt gctctagcta
     3061 aaacgttcgg cttaaaattt tgccgcgcta tttctgcttc aataatcttc cgtgtttttt
     3121 ctttcactaa atgcggtgaa tttatgacac gcgaaacagt aataacagaa aatccagtag
     3181 cttttgcaat atcttttaat gtagccatat gttttatttt atcattattt gattaataaa
     3241 aattacaata caaaacgaca acttaagctt tacgaatttt aatggtaacc ccttcgctat
     3301 tcatttcacc aatttcaata ctataaccaa ttacgctacc gtcattaaag gttgctcttt
     3361 ttaacaagta cttttgccaa ttattactag caataactgt ccctgtttga aatagcgctg
     3421 agtcgtccgc ttttactaaa ttatttgccc atgtttgcgc gcgtccgcga ccatcaagca
     3481 aatgcaattt atgtaaactg ttatgcgcat aattataact tggtgtattg gaagtcgcaa
     3541 aatccatcca tgaaccaccg ctaccatcta agtgcggcgg aattgtatca ctccactttt
     3601 tagtattacc gttatttgac aaaatcacta accgcgcatc aacatgccat atccgtaccc
     3661 caggaatcgt gtaacctttg acaccgttat agcctgaaac tgataagcta tcacgcgcat
     3721 ttaaaccggt tggtgtgtag tactcaatca aaatgtattc gtcataagca tgtccattcc
     3781 aagcgtcatt aataatgata aagtcaccac ttgattcagc gggtctaagc cttatatcac
     3841 tattaccaac cgggacatac ggtgtgctcc agtttgcgct aaatttagaa aacatattgt
     3901 gatcaataat attatgatcc atcatatcaa tcgctcccgc tggcgctata gtgccgtcat
     3961 aatcataata atcatcaaaa cttaagatat gaccactttc atgaatataa gtatgcgctg
     4021 ctggtttaag atatgtataa cctggcgctt caaccataaa ttcataactc gcccacccgt
     4081 aagcattaaa tactggttta tttttatcgg caaatctatt ccaaaaatat tggaaggccc
     4141 aaaaaagtgt atcatcactt tcataatcaa caggtgctaa gtacacaaag aaaacggaat
     4201 caacaaagcc atcattattt tggtcgtatt cttttaatgt tgatgaaaga taagtgttgt
     4261 acacctgttc aagcacatag tgactcttat cccagtactg ataaccgtca acagttttcg
     4321 ctaacaattc agtagtggaa taacccatat caaaaagggg cattacttca ccggtaagac
     4381 tgagtctacc ataacttgat ttatagtaat aagaggctac tgattcccaa cctgtatcac
     4441 tgctttcacc aaagaaagcg cgattaatcg ctccctgaat agtcgcctct cccccgtaac
     4501 ttgcgggtgt atcagtaaat ttaacaggta caaccatcat tttttggtta gaaactgaat
     4561 caaggtgcgc taaacccttc gtattagaaa tagcgtagtt attaattagt cgcgaattat
     4621 tgttaagcgg ttctacttta taataattat ctaaagtatc tggattcgcc gtaattgggt
     4681 gttcataatc attaacgccg tatgtcccgt ttaaaggata gggattttcg ctgccagtta
     4741 cttcggaagt tacttcattc gcacttgaac tagtcgcacc aaactcggac gactttaata
     4801 tatcaccaaa agtcacgcag cttgttaaga gtagagctgg gactactaaa attaatgctc
     4861 gacggataaa tgtacttttt ctcatcttaa tatatctcta tttctattgt cgcataagcg
     4921 ttagttaatt gtactatttt aaagcgaaaa ttaagcggtg tcttatcatt aaatgcgaaa
     4981 tttttaaaat attcatagcc aaactcatca cctttttgaa acaaagagtg ctgatttgct
     5041 tgattaaacg actcgctgat gttcgtgctt tcttttggct caagtaattt ataaagttta
     5101 ttcgtcttaa ttggtgcatt attataactt ccagtactat tgctatgcgc taaaagttca
     5161 gtaccgttaa attctccttc ataatcatta acgtaaggat acttaacaat actcccgttt
     5221 ttaatttcgt acaccgttct cgcgtcaacg tgatacacct tcactccgtt tgtctttggc
     5281 agtttaaagg aagcattaag cgttgaatca agctcattta acactgttgg tgtgtaatat
     5341 tcgagcaaaa tatattcatc atttgctttc tcgttccagt tatttttaag aacgagtaag
     5401 tcacctgttt cactaaaggg tcgaagttta attgtagtac ttctttttaa ggcgtaa
//
LOCUS       DAOSEX010000022         5422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_13356_length_5422_cov_2.423514, whole genome shotgun sequence.
ACCESSION   DAOSEX010000022 DAOSEX010000000
VERSION     DAOSEX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13356_length_5422_cov_2.423514"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PK612_00830"
     CDS             complement(<1..961)
                     /locus_tag="PK612_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94694.1"
                     /translation="MDIFTLLEFNEIVSKVKDLTKTELGGEYASLISPFESEEEATYA
                     LNLVSEAESILTRFGALPLAPSHKLAPLLEEARRGNILTPHDFYLISADAMNIVKINY
                     YLKDKLDNAPLLKAMLKREKDLAVLANEINKVITPNLLVKDNASHELARIRKNIALSE
                     DRLNKAAQGLISKYGNFLAENLITKRDGHFVIPVKTAYKNKVDGIIYDISNSGQTIFV
                     EPSEIAALNSTLISYQNDEIIEVNRILRALTLETLKYEDEIINNNILLAELDLIFAKA
                     TYGQSINGFVPVFQQTKGLQFKKARHPLIDKNLVVANTFNLKDD"
     gene            1032..1964
                     /locus_tag="PK612_00835"
     CDS             1032..1964
                     /locus_tag="PK612_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOH94695.1"
                     /translation="MHNYELRIPREDLGIVIEQTKLPSVSNKHSYMIIYYEKGNIKFS
                     IYDNTKNEPYKARFVGARLPKYLEPYLKEKDIKTPKSKSVTTKIVDKKITTFPHIGSD
                     EVGFGDFFGPLVVVAAYLDEELKALVESLNVKDSKALDDTKIVLLGNLLKDKIPHTKN
                     IVHNPKYNELIAQGYNMNQMKAMLHQNVLTTLAKRQKYDGKLYLDAFTSDKHYENYTA
                     LMSKAKVIQLKDGESNSLAIATASILARFYFLREIEVLNIRYNTKIPLGAGNAVDKFA
                     QEFLVKFGKNNLLSVVKQNFRNFKDLFADDGGLL"
     gene            complement(1927..2841)
                     /locus_tag="PK612_00840"
     CDS             complement(1927..2841)
                     /locus_tag="PK612_00840"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH94696.1"
                     /translation="MENKLLKVDFEHSGKRADVVLSSLLSITRAQVQKMIKDGDIKLN
                     GTSVKASYRIEGGEEFSVTIREKEAPNLTPYEIELDVRYEDEYLLVVNKPAGLVVHPS
                     FGHKKETLVHALLARGDKYSDVNGVFRPGIVHRLDKETSGLLIVCKNNEVHNKIAKLL
                     KKREITRRYTALVRGKVREEAGVIKTYLTRSKDNFKKMANTPATGKLAISHFKVKERF
                     KGYMLLEVELETGRTHQIRAHLEFINTPIVGDALYGLKKDPLYSKGQLLHASYLAFTH
                     PYTGELVEVSAELPDYFTKVLRRLQKDL"
     gene            complement(2831..3394)
                     /gene="lspA"
                     /locus_tag="PK612_00845"
     CDS             complement(2831..3394)
                     /gene="lspA"
                     /locus_tag="PK612_00845"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOH94697.1"
                     /translation="MNNVKQYLKSFFYSYAWLAVLLLIIDQLTKVWALKAKLDITLIP
                     NFLHLTYVRNTGAAWSMFDGNKLALALISFVVGTLLIVARIKYRTKLDTFKKILFAVV
                     LAGTWGNFIDRAFYKLLTGAEGVVDFIHFQFGNYHFPVFNVADICITLGILSYVVLLL
                     VEEFKALKAPKTEEKVKDDTNEEKDGE"
     gene            complement(3387..3791)
                     /locus_tag="PK612_00850"
     CDS             complement(3387..3791)
                     /locus_tag="PK612_00850"
                     /inference="COORDINATES: protein motif:HMM:NF018531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HOH94698.1"
                     /translation="MIKRLPSVHLMDFPYQLDVDNAKLEEFSALLKVRSNAYQHIEKA
                     RQEGVLGSSQEASLTLPHSEVELAPQLKKLDNEELARFFIVSEVKFCNTYKVEKHTGH
                     KCPRCWNYHQELVSVAEEQVCERCAKVLNTYE"
     gene            complement(3788..>5422)
                     /locus_tag="PK612_00855"
     CDS             complement(3788..>5422)
                     /locus_tag="PK612_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH94699.1"
                     /translation="MIWTTTPWTLPANLAISVHPAFTYGVYESALGDVILLPELVEEV
                     SKASDLTFTLKYEVKGADLDLIETKHPLYERISLVITGMHVTSESGTGAVHTAPGHGL
                     EDFVVGQKYGLDVLCPVDARGYFTSEAGKYEGLFYEDANKVIINDLREVGALVAHTKV
                     KHSYPHDWRTDKPLIFRATPQWFASIEPIRRDLLNAVQKVDWYPAWGEGRISNMISDR
                     GDWTISRQRVWGVPIPIIYNEDGSPILEEVVFDNIIAEVEKHGSNIWYEKDAKYFLPE
                     GYQNALSPNGNFTKEVDTMDVWFDSGISAIHTLKKNGLPFPADLYLEGNDQYRGWFNS
                     SLTLSVAALEKAPYKAVVTHGFVVDDKGEKMSKSKGNVLDPSKLANEYGADILRLWSA
                     TIDYQEDARLSVDLLRQTSETYRKIRNTFRFMLGNLPALDTSFAELKALYKEPDFYLD
                     KLMTNKLQQVINKMIDSYNDYDFGAAFTTLMNFVTNELSSFYLDVAKDILYCEAKDSA
                     RRQSVVGTLYKITVALLPLIAPVLVLPLKKFMSTYQ"
BASE COUNT         1687 a         1076 c          916 g         1743 t
ORIGIN      
        1 tatcatcttt taaattaaaa gtattagcaa cgacaagatt cttatcaata agcgggtgcc
       61 gggctttttt aaattgtaac ccttttgttt gttgaaaaac ggggacaaag ccattaatac
      121 tttgaccata agtcgcttta gcgaagatta aatcaagttc cgcaagtaag atgttattat
      181 ttataatttc gtcttcatat tttaaagttt ctaaagttaa tgctcttaaa atgcgattaa
      241 cttcaattat ttcatcattt tgataactaa ttaaagtact atttagtgcg gcgatttcac
      301 taggttcaac aaatattgtt tggccggaat tactaatatc ataaataatg ccgtcaacct
      361 tatttttata ggcggtttta acaggaatca caaagtgacc atcgcgctta gtaataagat
      421 tttccgctaa gaagtttccg tacttactaa ttaaaccttg cgctgcttta ttaaggcgat
      481 cttcactcaa ggcaatgttt tttctaatgc gcgctaattc gtggctcgca ttatctttta
      541 ctaaaagatt aggcgtaatt actttattaa tttcattagc gagcaccgct aaatcttttt
      601 cgcgtttaag cattgctttt aataagggag cattatctag cttatctttt aagtaataat
      661 taatcttaac aatattcata gcgtccgcac ttattaaata aaagtcgtgt ggtgttaaaa
      721 tattaccgcg ccgtgcttct tcaagaagcg gggcaagttt atgacttgga gcgagtggca
      781 acgcaccaaa gcgtgttaaa attgactctg cttcacttac taaattaagt gcgtaagttg
      841 cttcttcttc actctcaaaa ggactaataa gactagcgta ctcaccacct aattctgtct
      901 ttgttaaatc tttaacttta ctaacgattt cattaaattc taaaagtgta aatatatcca
      961 tatttgtatt ttatcattaa tgattatttt ctaataacga acaagattgt cgtttatgtt
     1021 aaaatatact catgcataac tatgaattac gaattccgcg tgaagattta ggtattgtga
     1081 ttgaacaaac aaaattacct agtgtttcaa ataaacattc atatatgatt atttattatg
     1141 aaaaaggaaa cattaagttt tcaatttatg ataatacgaa aaatgaacca tataaagcgc
     1201 gttttgttgg tgcgcgttta cctaaatatc ttgaaccgta cttaaaagaa aaagatatta
     1261 aaacgcctaa aagtaaaagc gttacgacta aaatagtcga taaaaagata acaactttcc
     1321 cgcatattgg cagtgacgaa gtcgggtttg gtgacttctt tggtccactt gtcgttgttg
     1381 ccgcttatct agatgaagaa ttaaaagccc tagtggaaag tcttaatgtg aaagattcaa
     1441 aagcgcttga tgataccaaa attgtcttac ttggtaattt actaaaagat aaaatccccc
     1501 acactaaaaa tattgtgcat aacccgaaat ataatgaact tatcgcacaa ggttacaata
     1561 tgaaccaaat gaaagcaatg ctccatcaaa atgtgttaac gacacttgct aaacggcaaa
     1621 aatacgatgg taaactgtat cttgacgcct ttacgagtga taagcattac gaaaactata
     1681 cggcactgat gagtaaagca aaagtcatcc aactaaaaga cggagaaagt aattcattag
     1741 cgattgctac tgctagtata ttagcgcgct tttatttcct tcgtgaaatt gaagtattaa
     1801 acattcgtta taatacaaaa atcccgcttg gagcgggaaa tgctgttgat aaatttgcgc
     1861 aagaattttt ggttaaattt ggtaaaaata atttattaag tgtcgtgaaa caaaacttcc
     1921 gtaattttaa agatcttttt gcagacgacg gaggactttt gtaaaataat ctggaagctc
     1981 tgcacttact tctactaatt ctccggtata cggatgcgta aacgctaaat aactagcgtg
     2041 taataattga cccttactat atagcgggtc tttttttaaa ccgtaaagcg cgtcaccaac
     2101 aattggcgta ttaataaatt ctaagtgcgc tctaatttga tgagtacggc ctgtttctaa
     2161 ttccacttct aaaagcatat agcccttaaa gcgttcttta actttaaagt gactaatcgc
     2221 tagtttacca gttgcgggag tattagccat ctttttaaaa ttgtctttac tgcgtgttaa
     2281 gtatgtcttt attacacccg cttcttcacg cactttaccg cgcacaagcg ctgtgtatcg
     2341 tctagtgatt tcacgcttct ttaagagttt agcaatttta ttatgcactt cattattttt
     2401 acatacgata agaagaccgc ttgtttcttt atcaaggcga tgaacaatac caggacgaaa
     2461 aacaccgtta acatcactat atttgtcacc gcgcgctaaa agggcatgca ctaaagtttc
     2521 ttttttatga ccaaaactag gatgcacgac taatcccgcg ggtttattga caacgagcaa
     2581 atattcgtct tcataacgca catcaagttc aatttcgtag ggtgttaagt ttggtgcttc
     2641 tttttcgcga atagtaacac taaactcttc gccgccttca atacggtaac ttgctttaac
     2701 gctggtgccg ttaagtttaa tgtccccgtc tttaatcatt ttctgcactt gcgcgcgcgt
     2761 aatacttaat aaagaagaaa gcactacatc agcgcgctta ccactatgct caaaatcaac
     2821 ttttaaaagt ttattctcca tctttttcct cattagtgtc gtctttaact ttttcttcag
     2881 tttttggagc ttttagcgct ttaaactctt ccactaataa taaaacgaca taacttaaga
     2941 tccctaaagt aatacaaata tcagcgacat taaacacagg gaaatgataa ttaccaaact
     3001 ggaaatgaat aaaatcaaca acaccttctg cacctgttaa aagtttatag aaagcgcggt
     3061 caataaaatt accccaagtt ccggcaagga caaccgcaaa aagtattttc ttaaatgtat
     3121 ctaactttgt gcgatactta atgcgcgcta caataagcag cgtaccgaca acaaaactaa
     3181 tcagtgctaa agcgagcttg ttaccatcaa acatactcca cgctgcgcct gtatttcgca
     3241 catatgttaa atgtaagaaa ttaggaataa gcgtgatgtc taacttcgcc tttagtgccc
     3301 atacttttgt taattggtca atgattaaaa ggagcacggc taaccaagca taggaataaa
     3361 agaatgattt taagtactgt tttacattat tcatatgtgt ttaatacctt tgcgcatcgt
     3421 tcacagactt gttcttcagc aacgcttact aattcttgat gataattcca gcaccgcgga
     3481 cacttgtgac cagtgtgctt ttctacttta taagtattac aaaacttaac ttcacttaca
     3541 ataaagaagc gtgcaagttc ttcattgtct aattttttta actgtggcgc aagttctact
     3601 tcactatgcg gtaaagttag gcttgcttct tgactactgc caagtactcc ttcttggcgc
     3661 gctttttcaa tatgttgata ggcgttgctt cgtactttta gtaacgcact aaattcttca
     3721 agtttagcgt tatcgacatc aagttgatag gggaaatcca ttaaatgtac ggaaggtaaa
     3781 cgcttaatca ctggtaagtg ctcataaatt tcttcagcgg taacactaat acaggagcaa
     3841 tcagtggtaa aagtgctacc gtaattttat aaagtgttcc cacaacactt tggcgtcgag
     3901 cgctatcttt agcttcgcag tataagatat ctttcgccac atcaagatag aaactactta
     3961 attcattcgt aacaaaattc atcagtgtag taaaagctgc cccaaagtcg taatcattat
     4021 aactatcaat cattttatta atgacttgtt gtaatttatt agtcattaat ttatcaagat
     4081 aaaagtctgg ttctttatat aatgctttaa gttcagcaaa acttgtgtct aacgcaggta
     4141 aattaccaag cataaagcgg aaagtattac ggattttacg ataagtttca cttgtttggc
     4201 gtaaaagatc aacactgagc cgtgcatctt cttgataatc aatcgtcgca ctccataagc
     4261 gtaaaatatc cgcaccatat tcatttgcta atttacttgg atcaagcaca ttacctttgg
     4321 atttagacat cttttcgcct ttatcatcaa caacaaagcc atgtgtcact acggctttgt
     4381 atggtgcttt ttctaaggcc gcaacgctta aagttaagga actattaaac caaccacgat
     4441 attgatcatt accttctaaa tataaatcag cgggaaaagg taaaccgttt tttttaagtg
     4501 tatgaatcgc actgatgccg ctatcaaacc acacatccat cgtgtcaact tctttcgtaa
     4561 agttaccgtt tggtgataat gcgttttggt acccttctgg taagaaatat ttcgcatctt
     4621 tttcgtacca tatattactg ccatgctttt ccacttccgc aataatgtta tcaaatacta
     4681 cttcttcaag aatcggacta ccatcttcat tataaataat cggaatgggg acaccccata
     4741 cgcgttgacg actaattgtc cagtcaccgc gatcactaat catattacta atccggcctt
     4801 caccccacgc tggataccag tcaacttttt ggacagcgtt aagtaaatct ctgcgaattg
     4861 gttcaatact ggcaaaccat tgcggtgttg cgcgaaagat aagcggttta tccgtccgcc
     4921 agtcgtgtgg gtaactgtgt tttactttcg tatgggcgac aagcgctcct acttcccgta
     4981 agtcattaat aataacttta ttagcgtctt cgtaaaatag tccttcatat ttcccagctt
     5041 cactagtaaa gtatcctcga gcgtcaactg gacataacac atctaagccg tacttttgcc
     5101 caaccacaaa gtcttcaagc ccgtgacccg gcgccgtgtg aaccgcccca gtcccgcttt
     5161 cactagtgac atgcatcccg gtgataacaa gggaaatacg ctcataaagc gggtgtttcg
     5221 tttcaattaa atctagatcc gcacctttga cttcatattt taatgtaaaa gttaagtcac
     5281 tcgctttact tacttcttcg acaagttcag gtagtaaaat aacgtcacct aacgcgcttt
     5341 cgtaaacccc atatgtaaag gcgggatgga cactaatcgc taagttagca ggaagcgtcc
     5401 acggggttgt tgtccaaatc at
//
LOCUS       DAOSEX010000023         5350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_13619_length_5350_cov_3.142398, whole genome shotgun sequence.
ACCESSION   DAOSEX010000023 DAOSEX010000000
VERSION     DAOSEX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13619_length_5350_cov_3.142398"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PK612_00860"
     CDS             <1..349
                     /locus_tag="PK612_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94700.1"
                     /translation="FYPFALDEKKLRKDAKLFVGTHNFQNFTSKKEDEANFVRNISAV
                     KIQKSGKMLKIVFIGEGFMRGQIRMMVGALLAQNAGKEDANFIKDNVNSKERKIISHK
                     VIGDGLYLRKVKY"
     gene            385..1101
                     /locus_tag="PK612_00865"
     CDS             385..1101
                     /locus_tag="PK612_00865"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH94701.1"
                     /translation="MGRAFQVRAASMASTAAKKSALNMRASKEIFMVAKSGGTDPNSN
                     LALRAALDKYRAMGATKDVMERAIKKAEGDDAFAYIPGRYEVMGPGGSQLVIDTLTDN
                     VNRAISDIKAVLKKTDSILQTVAYNFTETGIFLFAGNNLEEITETLILNDVDVWDINE
                     EDDVIEVLVNPTSYGKTRDTLYSLGIKEFNISEIQMIPNDKVTLNAEDTEVFKRIIDM
                     LEDVQDVQEVYHNVNVPTEE"
     gene            1140..1499
                     /locus_tag="PK612_00870"
     CDS             1140..1499
                     /locus_tag="PK612_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOH94702.1"
                     /translation="MRRNANPIFRNMNNDMNQAMTSSVPVDVTVSTASWSGIALKTLL
                     LVLIMIAAGLSTWFLPFPVVVGFLIFGGIASFFFVFFAMRSVKTAPVLSVLYAITQGI
                     MYGALTFIVETYFPVLV"
     gene            1499..1897
                     /locus_tag="PK612_00875"
     CDS             1499..1897
                     /locus_tag="PK612_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOH94703.1"
                     /translation="MALVATFGIVGVMAFLHSIKAVRATPGLIRFVFAALLMVMISSL
                     VLFIISRVAQDLYLSYVDNYPLMVIVSAFLVVLGAIMLILDFNNAETIVQSGAPKIYE
                     WQVSLGFLVTLVWIYLQVIRFLLVLARNRE"
     gene            2035..2976
                     /locus_tag="PK612_00880"
     CDS             2035..2976
                     /locus_tag="PK612_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002839737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94704.1"
                     /translation="MINMIKVKNLHKTYKKGVKAVNGLNFKVKAGEIFGLLGPNGSGK
                     TTTINAILDLVDYETGEIILFDNKYKTKVEAKKHIGLITQEVGVFNELTVRENIDYFC
                     GLYINDEKLRAQYVEEAMDFVQIKDFAKFKPKKLSGGLLRRLHIACGIAHKPTLIFFD
                     EPTVGIDPQSRNHILEQIKVLNKNGATIVYTSHYMEEIEMLCDYILIMDKGQVIAEGN
                     YDALIDQIDLAEYIEIEYKGEMPLKVVTEINELPGILEVIHTKEKLVIKTSEDDKAFI
                     KIIHILENNNVDYSRANANKPSLNDVFLELTGKELRD"
     gene            2978..4144
                     /locus_tag="PK612_00885"
     CDS             2978..4144
                     /locus_tag="PK612_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH94705.1"
                     /translation="MKSLFILNLKKLLKNKEIVFWALLFPIILSTIFHIVFDGIRNRD
                     NFETLKVNYVSTNFTAEDKAFLVEEMLTNKAEYIKNEGTDKAQTVKIFALSEKTKAEA
                     EKEFKKDKTIYFYKEDGAFKIKASSQNISTAILQTFIDDYQNFIKVTTLLVAESNGTL
                     SFQDAMNKHVASYAYTYDFDSGDKKIDYVNNYHYTVLAMAIIYGAFLATEQAKIFQPN
                     SFAFAKRVTVSGASRAKLLVSALLSSLVVQTAIIALYIAILVFIFKVTFANIGLALLA
                     ALIGVILVNINGFFIGIVFNKLSQNAVIAIIILAGTIGGFLSGMMISSMKFFVGTKMP
                     LLSYININALISDSFVSLDFGNMTRYWNNMLAMTVIGVILFGVTLIKFNRESNK"
     gene            4146..>5350
                     /locus_tag="PK612_00890"
     CDS             4146..>5350
                     /locus_tag="PK612_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH94706.1"
                     /translation="MGFKLFFKIVKANFVSFLAYVLILVVSGLMFGLSDSGAANEDFK
                     ILAPVYYANITNETVDDLPSDNTLIFDYLMDNNLISEESDDPFSQGFYTYTLNFVNVK
                     KNIKPSEVNDAIYAGVVRGALIIPKDLSTLTRENALFIFHTNSQDLATITPTKAALNK
                     FINTYHDLKEVSATAGNNLSATELTNKTLIALEKETEIVYENPNRTKLSMLSHFFSFS
                     TYIMSAVIILLVSIVMYEIKRREILMRISITPYSIPRLIVGLIFGAITLSLLLVGLVY
                     VLAIISYPKIALSITGLYLTLNIMLFTLPLTALAMVIGLVISKAEIVSIISTVFALAQ
                     AFFSGAFLPTFLLPKSIINVGKVFPASHTVTINNLLAEGLNTNGPSILLNIGILLAYF
                     AVFLTIAIIL"
BASE COUNT         1758 a          821 c         1024 g         1747 t
ORIGIN      
        1 tttttatcct tttgcattag atgagaaaaa gttacggaaa gacgctaaat tatttgtcgg
       61 tacacataac ttccaaaact tcacaagtaa gaaagaagat gaggctaatt ttgtccgtaa
      121 tattagtgca gtgaaaatac aaaagagcgg caaaatgcta aagattgtgt ttattggtga
      181 aggatttatg cgtggccaaa ttaggatgat ggttggcgca ttactcgcgc aaaatgcggg
      241 taaagaagat gcaaacttta ttaaagacaa cgtaaattca aaagaacgaa aaatcattag
      301 tcataaagta attggtgatg ggctttattt acgcaaagta aagtattagg cttataataa
      361 aatagtagtt aaaggagcaa agttatggga agagcatttc aagttagagc ggcaagtatg
      421 gcaagtaccg cagcgaaaaa gagtgcactt aatatgcggg cctcaaaaga aatttttatg
      481 gtggcaaaaa gcggaggcac tgatcctaat tccaacttag ccttacgcgc agcgcttgat
      541 aaatatcgag caatgggtgc gacaaaagat gtcatggaac gggcgattaa aaaggcggaa
      601 ggagatgacg cttttgctta tattccaggg cgttacgaag tgatgggccc aggtggcagt
      661 caacttgtaa ttgatacttt aactgacaat gttaatcgcg cgattagtga tattaaagct
      721 gtgcttaaaa agacggactc gatcttacaa acagttgctt ataactttac cgaaacaggt
      781 atctttttat tcgcaggcaa taatcttgaa gaaataacag agacattaat tcttaatgat
      841 gttgatgtat gggatattaa tgaagaagat gatgtgattg aagttcttgt taatccgact
      901 tcatacggta aaacacgcga tacattatat tctttaggga ttaaggaatt taacattagt
      961 gaaattcaaa tgattcctaa cgataaagta acactaaatg cagaagacac tgaagtattt
     1021 aaacgcatta ttgatatgct agaagatgtc caagatgtcc aagaagtgta tcataatgtt
     1081 aatgtgccca ctgaagagtg atataatatt agtggcaaaa atataaggag gaactttata
     1141 tgcgtagaaa tgcaaatcca atattccgta atatgaataa tgatatgaat caagcaatga
     1201 caagtagtgt gccagttgat gtgacagtta gcacagcatc atggagcggc attgccctta
     1261 aaacattatt actcgtactc attatgattg ctgcgggact tagtacatgg tttttaccat
     1321 tccctgtcgt cgtcggtttt ttaatctttg gtggtattgc ttcattcttc ttcgtgttct
     1381 tcgcaatgcg aagtgtgaaa actgctcctg ttttaagtgt gttatatgca ataactcagg
     1441 ggataatgta tggcgcactg acatttattg ttgaaaccta cttccctgtg ttggtgtaat
     1501 ggcacttgtg gcgacttttg gcattgttgg tgtgatggct ttcttacata gtattaaagc
     1561 tgtacgcgct acaccaggct taattcgttt tgtttttgca gccttattaa tggtaatgat
     1621 tagtagttta gtacttttca ttattagccg tgttgcccaa gatttatatt taagttatgt
     1681 tgataattac ccgctgatgg tcattgtcag tgcattctta gtcgtgcttg gggcgattat
     1741 gctcattctt gactttaata atgctgaaac aatcgtccaa agcggagcgc caaaaattta
     1801 tgaatggcaa gtaagtttag ggttcttagt tacccttgtg tggatttatt tacaagtcat
     1861 tcgctttctt cttgttctcg ctcggaatcg agaataagga atatatgtta gcaaaaagac
     1921 ggccactaca tgtggtcgtt ttttctttat tgtcgcgctt tactttgtaa agaaaccttt
     1981 acgaattgct tatactgata aaaattcaca taaatgaagt tataatgctt ataggtgatt
     2041 aatatgataa aagtaaaaaa cttacacaaa acatataaaa aaggtgttaa ggctgtaaat
     2101 ggtcttaatt ttaaagttaa agctggagaa atttttggct tattaggtcc taatggtagc
     2161 ggtaaaacga caacgatcaa cgctattctt gatttagtgg attatgaaac aggcgaaatt
     2221 attttatttg ataataaata taagactaaa gttgaagcca aaaagcatat cggacttatt
     2281 acccaagaag ttggtgtctt taatgaatta acagttagag aaaatattga ttatttctgc
     2341 ggactttata taaatgatga aaaactccgt gcacaatatg ttgaagaagc gatggacttt
     2401 gtgcaaatta aagattttgc aaagtttaaa ccaaagaaac tttcaggcgg attactccgg
     2461 agattacata tagcgtgcgg cattgcgcat aaaccaacgc ttattttctt tgatgaacca
     2521 accgttggga ttgacccaca gagtcgtaat cacatcttag aacaaattaa agtattaaat
     2581 aaaaacgggg cgacaattgt ttatacttcg cattacatgg aagaaattga aatgctatgt
     2641 gactatattt taattatgga caaaggccaa gttattgccg aaggaaatta tgatgcctta
     2701 attgatcaaa tcgatttagc ggaatacatt gaaattgaat ataaaggcga aatgccgcta
     2761 aaagttgtga cagaaataaa tgaattacca ggtattcttg aagtgatcca cactaaggag
     2821 aagttagtaa ttaagacgag tgaagacgat aaagcattta ttaaaattat tcatatttta
     2881 gaaaataaca atgttgatta ttcgcgggct aatgcgaata aaccaagttt aaatgatgta
     2941 tttttggagt taactggtaa agagttacgg gactaagatg aaaagtttat ttattttaaa
     3001 tttaaagaaa ttacttaaaa ataaagaaat tgttttttgg gcgttattat ttccaattat
     3061 attaagtact atttttcata ttgtgtttga tggtattcgc aatcgtgata actttgaaac
     3121 acttaaagta aattatgttt ccacaaactt tactgccgag gacaaagcgt ttttagttga
     3181 agaaatgctc actaacaaag ctgaatacat taaaaacgaa ggcacagata aagcgcaaac
     3241 agtaaagatc tttgcactta gcgaaaaaac taaggctgaa gccgaaaaag aatttaaaaa
     3301 agataagacc atttattttt ataaggaaga tggggcattt aaaattaaag catcttcaca
     3361 aaatataagt accgcgatat tacaaacatt tattgatgac tatcaaaact ttattaaggt
     3421 tacgacactc ttagtcgcag aaagtaatgg tacattatca tttcaagacg ccatgaataa
     3481 acatgttgca agctatgctt atacatatga ctttgattct ggtgataaaa aaatagatta
     3541 tgttaataac tatcactata cagtacttgc gatggcgata atttatgggg catttcttgc
     3601 gacagaacaa gctaaaatct ttcaaccaaa tagttttgct tttgctaaac gcgttacagt
     3661 tagtggcgct agtcgcgcta aattattagt gagtgcctta cttagttcac tcgttgtgca
     3721 aacagcaatt attgcgcttt acattgcaat attagttttc atttttaaag tgacatttgc
     3781 taatattggt ttagcgcttt tagctgcatt aattggtgtt attttagtga atataaatgg
     3841 ctttttcatt gggattgtct ttaataaact tagtcaaaat gcagtgattg ctatcattat
     3901 tttagcgggt actatcggcg ggttcctctc agggatgatg atatcatcaa tgaagttttt
     3961 tgttggcact aaaatgccgc ttttgtctta tataaatatc aacgcattaa ttagtgattc
     4021 atttgttagt cttgattttg gtaatatgac tcgttactgg aataatatgc tagcgatgac
     4081 cgttatcggt gtcattctct ttggtgtaac gcttattaaa tttaatcggg agagcaataa
     4141 ataagatggg gtttaaatta tttttcaaaa ttgttaaagc aaacttcgtt tcatttctcg
     4201 cttatgtttt aattttagtt gttagtggtc ttatgtttgg gttaagcgat agtggcgccg
     4261 ctaatgaaga ctttaaaatt ttagcgccag tctactacgc aaatattaca aatgaaacag
     4321 tcgatgactt accaagtgat aacactttaa ttttcgatta tttaatggac aataatttaa
     4381 ttagtgaaga aagtgatgat ccgttttccc aaggctttta tacatacact ttaaactttg
     4441 tgaatgtaaa gaaaaatatt aagccaagtg aagtaaatga cgctatttat gccggagttg
     4501 tgcgtggcgc actcattatt ccgaaggatt tatcaacttt aacgcgggaa aatgcattat
     4561 ttatttttca tacgaattcg caagatttag cgacaataac cccaacaaaa gccgccctta
     4621 ataagtttat taatacttac cacgacttaa aagaagttag tgctacagcc gggaataact
     4681 tatccgctac tgagttaaca aacaaaacat taattgcgct tgaaaaagaa acggagatag
     4741 tttatgaaaa tccaaatcgc acaaaactgt cgatgttaag ccatttcttt tcattttcaa
     4801 cttacattat gtcagcagtt attatcttgc ttgttagtat tgtaatgtat gaaattaaac
     4861 gccgtgaaat attaatgcgt atttcgataa cgccctacag catcccgcgc ttaatcgtgg
     4921 gccttatctt tggagcaata accctttcgc ttctgttagt aggcctagtt tatgtcttag
     4981 caattatttc ttatccaaaa attgctttaa gcataactgg attatactta acacttaata
     5041 ttatgctatt tactttacca cttacggcac tagcgatggt cattggcctt gtaattagta
     5101 aagctgaaat cgtctccatc attagtacag tctttgcttt agcgcaagcc tttttctctg
     5161 gagctttttt accaacattc ctcttaccta agtcgattat taatgttggg aaagtctttc
     5221 ccgctagtca tacagtgaca attaataact tgcttgccga aggattaaat acaaacggac
     5281 caagcatctt attaaatatc ggtattttac tagcctactt tgcagtattc cttacaattg
     5341 ccatcattct
//
LOCUS       DAOSEX010000024         5335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_13669_length_5335_cov_3.588447, whole genome shotgun sequence.
ACCESSION   DAOSEX010000024 DAOSEX010000000
VERSION     DAOSEX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13669_length_5335_cov_3.588447"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1156)
                     /locus_tag="PK612_00895"
     CDS             complement(212..1156)
                     /locus_tag="PK612_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOH94707.1"
                     /translation="MNTIKFLLTDGQIFGIIALAVLLLFIFIVLIRSIKIVRQATVLV
                     VERLGKYNRICHAGIHFIIPFFERASQPIFLKERVSDFPPQPVITKDNVTMSIDTVVY
                     WQVTDPKLFMYGVISPQSALENLTATTLRNLVGDLELDDTLTSRDTVNHQMWEVLDLA
                     TDPWGIKVTRVELKNILPPRDIQEAMEKQMRAERQKRQEILEAEGKKQAAILIAEGEK
                     EAVILRAEAAKQKMIKEAEGEAAALLAVYEANAKGIDLINKSNPAPGYLTLKGFEALE
                     NLARGQATKIIVPSEIQNVTGLLTSLKEAVVDEKPKEK"
     gene            complement(1156..1632)
                     /locus_tag="PK612_00900"
     CDS             complement(1156..1632)
                     /locus_tag="PK612_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOH94708.1"
                     /translation="MIQILFLADANWYFGLWLGIFILAIIIEAAEPGLVSIWFAGGAL
                     VAFILSLFPNVPLWVQIIAFIATSGILLLLSFLFFRKSFLNAKSVQTNVDAMLEEEVI
                     LLSSCDKDTLGEVKYRDVIWKVTPKSHDASFEKGETAIVDGIKGNRLVIKKKGTKK"
     gene            complement(1700..2299)
                     /locus_tag="PK612_00905"
     CDS             complement(1700..2299)
                     /locus_tag="PK612_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH94709.1"
                     /translation="MENISKVIANNLIFLRRKHNLTQQELAEKINYSDNAVSRWERGE
                     ATPSVETLALIADYFAINVADLLDENFPTKNAPKQKGKRVQRIFVILFSVSIVWTFAL
                     IGFIYTQMFKISLGNYGENGWLLFVMSVPISCLVLYFYNKLWGNKVYHLIIFSVFWWS
                     IITTIYLHILVLTGVNLWLIFLLGIPFELAQVLWFFIRR"
     gene            2461..3234
                     /locus_tag="PK612_00910"
     CDS             2461..3234
                     /locus_tag="PK612_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94710.1"
                     /translation="MQKTKTIYYSDPLNEDFGPTPHNLKKITGNYRYNRDNIFIRFFD
                     FLTYRFILTPIAFIYTKLIARIKLANKHLLKDLKKEGVIVYANHTHAQADAFGPPVHF
                     FPKKVTLVTNSANVSLPILGNLTKAWGAFPLPEDYTASKRFVKEFALRLKRKGVVIIY
                     PEAHLWPFYTGIRPFTHNNFAYSVKFNVKSYALTSTYQKNKKGKLKRIVYLDGPFTVD
                     EKLSSKDASVKLRDAIYEVMKKRSKLSTYEKIRYVKRSE"
     gene            3234..4046
                     /locus_tag="PK612_00915"
     CDS             3234..4046
                     /locus_tag="PK612_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH94711.1"
                     /translation="MNIVFAGNYKVFKGMLLGTLSIIKYYKEPLNLYILTMELKEQDP
                     DYIAVSDEDIKLLEKVVKDVNKKSTVTKIDVTGLYIEHLLESPNSKTRYTAYTLLRLL
                     LDEVAGIPLKVLYLDTDVLANDDISLLYNTDISNYELAGVKDRYGRFFMGPRYLNAGV
                     LLLNMQKIKETGLFTKCRAYLNKKEVFLSDQTAINKYVKKKLILKRRFNEQKQVKKDT
                     VIRHFSMQFRLFPKFHFVNIKPWHKDRLHKEYKCHHFDDILEKYEAITKEKL"
     gene            4050..4994
                     /locus_tag="PK612_00920"
     CDS             4050..4994
                     /locus_tag="PK612_00920"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HOH94712.1"
                     /translation="MKKEIPIFFACDDNYIPLLGNALYTLLKHASEDYHYTVIVLNAG
                     LKKRNKERIKEFETKHDNLTVKFANISKLINEHYTDLAYRLRDYYSVAIYFRMFIPTM
                     FPEYDKAIYLDADLILVGDISELYNTNIEGKLVAAINDKLVSSVPTFAAYANNAVGIS
                     ADEYFNSGVLVMNLKKFREDDIETKFLDLLKTYNFDTVCPDQDYLNVLCRNDKVLLHT
                     GWNKMPLEEPGFDYTTLKLLHYNSFEKPWHYDHVRYGDKFWEESAGSPFYEDLLKIRN
                     SVDENHIKKGKQGAAALLEKALYLAEQDEGTFKKTLAL"
     gene            5003..>5335
                     /locus_tag="PK612_00925"
     CDS             5003..>5335
                     /locus_tag="PK612_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94713.1"
                     /translation="MKDIFAALRPQVNKNIEKAIKDGDLNRKVMPGDHVVTPLERAKY
                     IVTYDLEKKRLRSKVKRIFGNIVANKKTRDIAKYIDISGLENASPLKGKAFIMTSNHY
                     SPYDNLIAR"
BASE COUNT         1815 a          896 c          946 g         1678 t
ORIGIN      
        1 ttaatatcta ttgtcaatag aaaaagtgtt gaaaaagtgc acttttttgc tactttttgt
       61 aggaaaaaca gccaaaaaac gcaaaagatt agattataca ataatatttt gatttggaat
      121 aatattccag catttagtga atattcttat agaaaatata aaaagcagac taataatagc
      181 ctgcttagta tttattattt attaataata actatttttc ttttggtttt tcgtcaacga
      241 cggcttcttt taaggaagtt aagaggccag taacgttttg aatttcactt ggcacgatga
      301 tcttagtggc ttgcccacgg gcaagatttt ctaatgcttc aaagccttta agtgttaagt
      361 agccaggcgc aggattagat ttattaatta agtcaatccc tttagcgtta gcttcataga
      421 ccgctagaag ggcagcagct tcaccttccg cttccttaat cattttttgc tttgcggctt
      481 cagcgcgtaa gataacagct tctttttccc cttccgcgat taaaatcgcg gcttgctttt
      541 taccctcagc ttcaagaatt tcttggcgtt tttgccgttc cgcgcgcatt tgtttttcca
      601 ttgcttcttg aatatctcta ggtggaagaa tgtttttaag ttcaacacgt gttactttaa
      661 taccccatgg gtcagttgca aggtcaagaa cttcccacat ttgatgatta acagtgtcac
      721 gacttgtgag tgtatcatct aattctaagt cccccactaa gttacgtaat gtcgtagcgg
      781 tcaagttttc taatgcactt tgcggagaga taactccgta cataaataat tttggatcag
      841 ttacttgcca atacacaaca gtgtcaatac tcattgtgac attgtcttta gtaataacag
      901 gttgcggtgg gaagtcactc accctttctt ttaagaagat tggttgactt gctcgttcaa
      961 aaaagggaat gataaagtga atgccagcgt gacaaatacg attgtattta cctagtcttt
     1021 caacgactaa gacagttgct tggcgtacaa ttttaattga acgaattagc acaataaaaa
     1081 taaatagtaa taagacagca agggcaataa ttccaaaaat ttgtccatct gtaagtaaga
     1141 atttaatagt gttcattatt ttttagttcc tttctttttt ataactaagc gattaccttt
     1201 gattccatca acaattgcgg tttcgccttt ttcaaaacta gcgtcatgac tttttggtgt
     1261 cactttccaa ataacatcac gatattttac ttcgccaagt gtgtctttat cgcatgaaga
     1321 tagtaagatt acttcttcct caagcatagc atcaacatta gtttgcacac ttttagcatt
     1381 taaaaatgac ttgcggaaaa agagaaaact aagtagtaat aatattccgc ttgtggcaat
     1441 aaaggcaatt atttgtaccc aaagtgggac atttgggaat aaactaagaa taaaagcgac
     1501 aagcgcgcct cctgcaaacc aaatactaac tagaccaggt tctgcggctt caataatgat
     1561 tgctaaaata aaaataccga gccataaacc aaagtaccaa tttgcgtccg cgaggaagag
     1621 tatctgaatc atagatggtg tcctccttta gtattattat atcatatata tgtgtaaata
     1681 atctttaaat attacatatt taacgcctaa taaaaaacca tagtacttgg gcgagttcaa
     1741 aaggaatccc aagtaagaat attaaccaaa ggtttacccc tgttaataca aggatatgga
     1801 gataaatagt agtaataatt gaccaccaga aaacactgaa gatgatgagg tgataaactt
     1861 tatttcccca tagtttatta tagaagtaga gtacaagaca agaaatgggg acactcatga
     1921 cgaataaaag ccacccattt tctccgtagt tcccaagact aattttaaac atctgtgtat
     1981 agataaaacc aattaacgca aaggtccaca caattgaaac gctaaaaaga ataacgaata
     2041 tccgttggac tcttttgcct ttttgtttag gcgcattttt agtggggaaa ttttcatcca
     2101 aaagatcagc gacattaatg gcaaaataat cagcaattaa agcgagtgtt tcgacacttg
     2161 gtgttgcttc accacgttcc cagcggctca cagcattatc actataattt attttttcgg
     2221 ctaattcttg ttgtgttaag ttatgtttgc gacgtaagaa gatgagatta ttagcgatta
     2281 ctttactaat gttttccata aactatgctc ctttacattc gtaaatatag tagcaattat
     2341 gggtgttttt gtcaaattcc cagattttga gaattagatt cttaaacatt gataacgcaa
     2401 attcaacttt tcccttccta aatttcccct tcatatttaa catatgaacg ggtgaacgtt
     2461 atgcagaaaa cgaaaacgat atattacagt gatcccctta atgaggactt tggtcctacg
     2521 ccacataatt taaaaaagat tactggtaac tatcgatata accgtgataa cattttcata
     2581 cggttttttg attttctcac ttatcgtttc attctaactc ccatagcgtt catctatacg
     2641 aaacttatcg ctcgtattaa attagcgaat aaacacttac taaaagactt aaaaaaagaa
     2701 ggagtgattg tctacgctaa tcatacacat gcacaagctg atgcctttgg accaccagta
     2761 cacttctttc caaaaaaggt aacactcgtc acgaatagtg caaatgtatc cctaccgatt
     2821 cttggaaatt taacaaaagc gtggggtgcc ttcccgttac cagaagacta tacagcgagt
     2881 aagcgttttg tgaaggagtt tgcattaaga ctcaagcgca aaggagtggt cattatttat
     2941 ccagaagcgc atctttggcc tttctatact gggattagac catttaccca taacaacttt
     3001 gcgtattccg tgaagtttaa tgttaaaagc tatgccttaa catctactta tcaaaaaaac
     3061 aaaaaaggaa agcttaaacg tattgtgtat cttgacggac cgtttactgt tgatgaaaag
     3121 ttaagtagta aagacgcaag tgtgaaactg cgtgacgcga tttatgaagt gatgaaaaag
     3181 cgaagtaagt tatcaaccta tgaaaaaatt agatatgtaa agaggagtga ataatgaata
     3241 ttgtatttgc cggtaattat aaagtattta aaggaatgct cttaggcact ttaagtatta
     3301 tcaagtacta taaagaacct ttaaatttat atattttaac aatggagtta aaagaacaag
     3361 atccagatta cattgctgtt agtgatgaag atattaaatt attagaaaaa gtagtgaaag
     3421 atgttaataa aaagtctact gtcacgaaga ttgacgtaac aggactttat attgagcatc
     3481 tccttgaaag tccgaatagt aagacgcgct acactgctta tacattgtta cgtttattat
     3541 tagatgaagt agcgggtatc cctcttaaag tgctttattt agacactgat gttttagcaa
     3601 acgatgatat aagccttctt tataacaccg atattagtaa ttacgaactc gcgggtgtta
     3661 aagatcgtta tggtcgtttc tttatggggc cacgctattt aaacgctggt gttttattac
     3721 ttaatatgca gaagattaaa gaaactgggt tatttactaa atgtcgtgct tacttaaata
     3781 aaaaagaagt atttttgagt gaccaaacag cgattaataa gtatgtcaaa aagaaattaa
     3841 tcttaaaaag aagatttaat gaacaaaagc aagtaaaaaa agacacagtt attcgccatt
     3901 ttagtatgca atttagactt ttcccaaagt ttcattttgt gaatattaaa ccgtggcata
     3961 aagatagatt acataaagag tataagtgtc accattttga tgacatttta gaaaaatatg
     4021 aagcaataac aaaggagaaa ttataaatta tgaaaaaaga aattccaata ttttttgcgt
     4081 gtgatgataa ctacattccg cttcttggta atgcccttta taccctttta aagcatgcga
     4141 gtgaagatta tcactataca gttatcgtgc ttaacgctgg gcttaaaaaa agaaataaag
     4201 aacgaatcaa agaatttgaa actaaacacg ataacttaac agttaaattt gcgaacatta
     4261 gcaaactaat taacgagcat tacacagatt tagcttatcg ccttcgtgat tattatagtg
     4321 tcgctattta tttccggatg tttatcccga cgatgtttcc tgaatacgat aaagccattt
     4381 atttagacgc tgatttaata ttagtcggag acattagtga actatataac acaaacattg
     4441 aaggcaaact agtcgccgcc attaatgata aattagtaag tagtgttccc acattcgcag
     4501 cttatgcgaa taatgcagta ggaatcagtg ctgatgaata ttttaattct ggtgtgctcg
     4561 taatgaatct taagaaattt agggaagatg atattgagac aaagttttta gatttactaa
     4621 aaacatataa ttttgatacg gtttgtcctg atcaagatta tttaaatgtt ttatgccgta
     4681 atgataaagt tttactccac actgggtgga ataagatgcc actagaagaa cctggttttg
     4741 attacacaac acttaagcta ttacactata actcatttga aaaaccatgg cactatgacc
     4801 atgtccgtta cggtgataag ttttgggaag aaagtgcagg cagccccttt tatgaagatc
     4861 ttttaaagat tagaaatagt gttgatgaaa accatattaa gaaaggaaag caaggcgcag
     4921 cggccctctt agaaaaagca ctctatcttg cagagcaaga cgaaggcact tttaagaaga
     4981 cacttgcgtt ataaatagcg ttatgaaaga tatatttgcc gccctccgac cccaggttaa
     5041 taaaaatatt gaaaaagcaa ttaaagacgg tgatttaaac cgtaaagtca tgcctggtga
     5101 ccatgttgtg acaccacttg agagagcaaa gtacattgtt acttatgatt tagaaaagaa
     5161 aaggttacgc agtaaagtta aacggatttt tgggaacatt gtcgctaata agaaaacaag
     5221 agatatcgct aaatatattg acattagtgg tttagaaaat gcttcaccct taaaaggaaa
     5281 agcttttatt atgacaagca atcactattc gccctatgat aacttaattg ctagg
//
LOCUS       DAOSEX010000025         5276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_13888_length_5276_cov_3.062632, whole genome shotgun sequence.
ACCESSION   DAOSEX010000025 DAOSEX010000000
VERSION     DAOSEX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13888_length_5276_cov_3.062632"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PK612_00930"
     CDS             complement(<1..544)
                     /locus_tag="PK612_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94714.1"
                     /translation="MMKKRLMTLPLIMLLLSGLKSPISNIEKLNAATPMPTTIVFENN
                     TDAEVSAYYTGVEGKTGDELLEFLYSVIKDHNEYDYENNTHRYIYKIIDRNWELDAID
                     TVSPANLENFDYVNDNGFIHKLYADYNDDINTADRFKNDGASRVSFDKEHIWAKSLGN
                     FGRTRGAGSDFHSLWPADVKG"
     gene            672..2012
                     /locus_tag="PK612_00935"
     CDS             672..2012
                     /locus_tag="PK612_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOH94715.1"
                     /translation="MNKKEKKLKPGIKKKMSPYLIIVLSFLSVIIIGALLLSLPFAKQ
                     EGVTLSFLDTFFISTSAVTITGLSPIHNLSTTFSVFGKVVIALLTQIGGLGIITLSVF
                     VMILIGMKIGISNRVLLQTNLNSPSMAGVVNLIKKIVIFTLIVEFIGFLINLAVFIPL
                     FPLPKAIGISAFHAIASFNNSGFDILGDSSLINYQDNILLLINTALMIMIGGLGFIAI
                     QDIFTKRSIKKLSIHTKIVIKVNIFLWIVGTLVIKLAQIGGESTSWLTAFFMSVSART
                     AGFAAEKLVNFHSLSLLMLICLMFIGGSPSSTAGGIKTTTFYTLTKSMISYGLGKPTT
                     TYNRVIDQESKNKAFLLLFTALTGVVIGTALILFFDNLTIGRALFETVSALSNVGLSV
                     GTTATLSSASKVVLIVLMFAGRVGPLTIMSLMNRDWYKIGAHPVDYLEEKIIIG"
     gene            2022..2684
                     /locus_tag="PK612_00940"
     CDS             2022..2684
                     /locus_tag="PK612_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOH94716.1"
                     /translation="MKSIAIIGLSRFGLSLVEAFSKLDVELVAIDMDEEQVRKAGDFI
                     QNVFIADSTSIESLRAAGIANVDHAIVAIGQNERINLTTSIISVIKLKELGIKEITAR
                     ADEEDYAEVLTLVGATNIVMPLDIAAERIANKIAAGNVLDYFNVRRDYDVYEVKISDD
                     FEPLPIIKLDSRTRYKINILLIERNKVLYVPTKDTILEPGDEIFVFGKKQDIPKVLSV
                     FN"
     gene            2782..4005
                     /gene="ugpC"
                     /locus_tag="PK612_00945"
     CDS             2782..4005
                     /gene="ugpC"
                     /locus_tag="PK612_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOH94717.1"
                     /translation="MAKVKLSGIYKRYEGNKNFSVSDFNLEIGEKEFIVFVGPSGCGK
                     STTLRMIAGLEDITDGELYIDGVLVNDLPPKDRGIAMVFQSYALYPHMTVFDNMAFGL
                     KVQRLPKDEIKRRVHEAARILEIEHYLQRKPRALSGGERQRVALGRAIVRQTEVVLMD
                     EPLSNLDAKLRVQMRSEIIKIHETLGSTTIYVTHDQTEAMTMASRIVVMKDGFIQQVG
                     SPIEIYHNPANLFVASFIGSPPMNFFNVVVQKDHLEFVHYEEREEETEDGEDKVELVS
                     VKERVVDGKLPIPASLAKKLKGYVGKEVVFGVRPENVNYATSEGGREYADFKQNFTVE
                     IAELLGNEYIVHSKLFGQKIISKLLVDPANAIHIGAKLDLVIDFDKVAFFKVDTEAEA
                     AELPSAELNKRIELK"
     gene            4210..>5276
                     /locus_tag="PK612_00950"
     CDS             4210..>5276
                     /locus_tag="PK612_00950"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH94718.1"
                     /translation="MFKQKRIALLVAGLLLLAGCNGGSSADVSGDVSQGTTSQTTTTS
                     GGGSKERVRLSVWGSSAEEPLYHKHAAEFQEKHPEINFSISYADVGEADAATNVLQDV
                     STAADVFFFADDQLPTMQAREVIAKLPKGYANKVKERDVASAAENASSAVDDELYAFP
                     VTNDNGYMLIYNKAFLTETDVETLEGIMAKTTAEHQFVVDMGNGYYATSFIQYLCDIT
                     YNPITRLHTTNFNSAEAVDALQGVSNLLKPRVDNGFKSVDFNGAALDDLSDANGNKVI
                     AAVTGVWNTELLKEKLGENLGAAKLPTFKSANGTTVQWGSFAGSKLVGVKSSSNNLGW
                     ALAFADYNTDEAAQRYRFETNG"
BASE COUNT         1696 a          937 c          985 g         1658 t
ORIGIN      
        1 tacctttaac atcagcaggc cataatgaat gaaaatcact acctgctcca cgagtgcgac
       61 caaagttacc aagtgacttt gcccaaatat gttctttatc aaaactgact cgacttgccc
      121 catcgttttt aaaacgatca gcagtattaa tatcatcatt atagtcagcg tataatttat
      181 gaatgaatcc attgtcattg acatagtcaa aattttctaa attcgctggt gatacggtat
      241 caattgcatc taattcccaa ttacgatcaa tgattttata aatatatcga tgcgtattat
      301 tctcatagtc atactcatta tgatctttaa taacagagta gagaaattca agtaattcgt
      361 ctcccgtttt tccttcaaca ccagtgtaat atgcacttac ttccgcgtca gtgttatttt
      421 cgaaaacaat cgtcgtcggc atcggtgttg ctgcattaag tttttcaatg tttgatatcg
      481 gggattttag accacttagt aataacataa taagtggcaa ggtcattaat ctttttttca
      541 tcattaactc caatcttctt attaaattaa catttaaatt ataccattta tccttaagta
      601 acaaagaagg gcgatatttt atttagtgtt atctttaaca ctttcatgaa taaaagcata
      661 aaatataaaa tatgaataaa aaagaaaaga aattaaaacc aggtattaaa aagaaaatgt
      721 caccatatct aatcattgtg ctttcttttt taagtgtaat tattattggt gccttgttgc
      781 tttcattacc gtttgcaaag caagaaggag taacactctc atttttggat actttcttta
      841 tttctacttc tgcggttaca attacaggtt tgtcacccat tcataattta tccactacat
      901 tttcagtatt tggtaaagtt gtaattgcac tattaacaca aataggtggg cttggcatca
      961 tcactttaag tgtgtttgtg atgattttaa ttgggatgaa aattgggatt tctaatcgtg
     1021 ttcttttaca aacaaatctt aactcaccaa gtatggcggg tgttgttaat ctaattaaga
     1081 aaattgtcat atttacactc attgttgaat ttatcggttt tcttattaat ctagccgttt
     1141 ttatcccgtt atttcctttg ccaaaagcga ttggtataag tgcttttcac gccatagcgt
     1201 catttaacaa ttcaggtttt gacatattag gggatagctc tttaataaat tatcaagata
     1261 atattctctt actaatcaat actgcactga tgattatgat tggtggtctt ggctttattg
     1321 ctatccaaga tatatttaca aaacgttcaa ttaagaaatt aagcatccat acaaaaatag
     1381 taattaaagt taacattttc ttatggatag ttggtacttt agtcatcaaa cttgcgcaaa
     1441 ttggcggtga atcgacaagt tggcttactg ctttctttat gagcgtgagc gcgagaaccg
     1501 ctggttttgc tgctgaaaaa ttagtaaatt tccatagttt aagtttatta atgcttattt
     1561 gtttaatgtt tatcggcggt tcacctagtt ccactgctgg gggtataaag acaacaactt
     1621 tttatactct tacaaaaagt atgattagct acggacttgg gaaacctact acaacttata
     1681 accgtgttat tgaccaagaa tcaaaaaata aggcgttttt gctgctgttt accgcattaa
     1741 ccggcgttgt aataggaacc gcgctaatct tattctttga taatttaact attggacgag
     1801 ctctttttga aactgtatct gcattgtcta atgtgggctt atcagtcgga actacggcga
     1861 cactaagcag cgctagcaaa gtcgttttaa ttgtacttat gtttgcagga agagttggtc
     1921 cgctaacaat tatgtcactg atgaatcgcg actggtataa aattggagcg catccagtag
     1981 attatttaga agaaaaaata attataggtt aaaatatatt tatgaaaagt attgcaatta
     2041 ttggtttaag tagatttggc ttaagtttag tagaagcttt tagcaagtta gatgttgaat
     2101 tagtcgctat tgatatggac gaagagcaag tccgtaaggc cggcgacttt attcaaaatg
     2161 tttttattgc tgattccact agtattgagt ctttaagagc tgctggtatc gctaatgttg
     2221 atcatgcaat tgtcgcgata ggacaaaatg aaagaatcaa tttaactact agcattattt
     2281 cagtcatcaa attaaaagaa ttaggcatta aagaaattac cgctcgtgct gacgaagaag
     2341 attacgcaga ggttttaaca ttagttggtg cgactaatat cgttatgcca cttgatatcg
     2401 cagcggaaag aatcgctaat aaaatagcag ccggtaatgt tcttgattat ttcaatgtcc
     2461 ggcgcgatta cgatgtttac gaagttaaaa taagtgatga ctttgaacca cttccaatta
     2521 tcaaacttga ctcacgtacc cgctataaaa taaatatatt gttaatcgag cgcaataaag
     2581 tgctctatgt tccaacaaaa gatacgatat tagaaccagg tgacgaaatt tttgtttttg
     2641 ggaaaaaaca agacattcca aaggttctta gtgtctttaa ttaatcttct tcttggttat
     2701 gaaaatttta aatataacat cgattggcac aaatttaaaa atgtgtgcta tgattaaatt
     2761 aattgaagaa ggagttttat tatggccaaa gtaaaattat caggcattta caaacgttac
     2821 gaaggtaaca aaaacttttc ggttagtgat tttaacctag aaatcggtga aaaagaattt
     2881 attgtttttg ttggtccatc aggctgtggt aagtccacaa ctttacggat gattgctggg
     2941 ttagaagata ttaccgatgg ggaactttac attgacggag tgctcgttaa tgacttaccc
     3001 ccgaaagacc gcggaattgc aatggttttc caaagctatg cactttatcc gcatatgact
     3061 gtgtttgaca acatggcatt tggactaaaa gtccaacgtc tcccaaaaga tgaaatcaaa
     3121 cgccgtgttc atgaagcagc aaggattctt gaaatcgaac attatctcca aagaaaacca
     3181 cgtgcgctct ctggtggtga aagacaacgg gttgctttag gacgtgcaat tgttcgtcaa
     3241 actgaagttg tattaatgga cgaaccttta tcaaacttag acgcgaaact tcgtgtgcaa
     3301 atgcgttcag aaattattaa aattcacgaa acattaggta gtacaacaat ttatgttact
     3361 cacgaccaaa ccgaagcaat gacaatggcc tcacgtatcg ttgttatgaa agacggattc
     3421 atccaacaag ttggttcacc aattgaaatt tatcataatc cagcgaactt attcgtcgct
     3481 agtttcattg gttccccacc gatgaacttc tttaatgttg ttgtgcaaaa agaccatctt
     3541 gaattcgttc attatgaaga acgcgaagaa gaaactgaag atggcgaaga taaagtcgaa
     3601 ctcgtcagtg ttaaagaacg tgtcgttgat ggtaaattac caattcctgc tagtttagcg
     3661 aaaaaactta agggttatgt tgggaaggaa gtcgtctttg gcgtccgtcc agaaaatgtc
     3721 aactatgcca caagcgaagg tggccgcgaa tacgctgact ttaaacaaaa cttcactgtt
     3781 gaaatcgctg aattacttgg taacgaatat atcgttcact ccaaattatt cggtcaaaaa
     3841 attatttcga aattactcgt cgatcccgct aatgcaatcc acattggggc aaaacttgac
     3901 ttagtaattg actttgacaa agtcgctttc ttcaaagttg ataccgaagc agaagctgcc
     3961 gaattaccga gcgctgaatt aaataaacgt atcgaactca agtaataatc aacaaaattc
     4021 gagaaaaagc tccgctaaca ggagcttttt ttgattaaaa tcaaaaggtt tccaaattat
     4081 atacatttat catttgtcaa aatataaaat aattactata atgactatgt ctaaacttaa
     4141 atattggcag agcttaacta tcgttagcat ttttaggctc tcccacaaaa caatgagaaa
     4201 ggagaattta tgtttaaaca aaaacgtatc gctttacttg ttgcaggttt gcttttactt
     4261 gccggctgta atggtggcag tagcgctgat gtcagtggtg atgttagtca aggaacaaca
     4321 agccaaacaa caacaacgag tggaggcgga tcaaaggagc gtgtccgtct atcagtgtgg
     4381 ggctcaagtg ctgaagaacc actctatcac aaacacgctg ctgaattcca agagaagcat
     4441 ccagaaatta acttctcaat tagttacgct gacgttggag aagctgatgc cgcaacaaac
     4501 gtccttcaag acgtaagtac tgctgctgac gtatttttct tcgcagatga ccaacttcca
     4561 acaatgcaag ctcgtgaagt tatcgcaaaa ttaccaaaag gttatgcgaa taaagttaaa
     4621 gagcgtgacg ttgctagtgc tgccgaaaac gcaagttcag cagtggacga tgaactttat
     4681 gcattccctg ttacaaatga caacggctat atgcttattt acaataaggc attcttaaca
     4741 gaaaccgacg tcgaaacttt agaaggaatt atggcgaaaa ctactgccga acatcaattt
     4801 gtcgttgata tgggtaatgg gtattatgca acttccttca ttcaatatct ctgtgatatc
     4861 acttataatc caattacaag acttcatacc actaacttca acagtgctga agcagttgac
     4921 gccttacaag gtgttagcaa tctcttaaaa ccaagagtag acaacggatt taaatcagtt
     4981 gactttaacg gtgctgcact tgacgactta agtgatgcca atggtaacaa agttatcgct
     5041 gctgttactg gtgtgtggaa taccgaactc ttaaaagaaa aattaggtga aaaccttggt
     5101 gccgctaagc tcccaacatt taagagtgca aacggaacaa ccgtacaatg gggttccttt
     5161 gctggttcca aattagttgg tgttaaatct tctagtaata acctcggatg ggcacttgcc
     5221 tttgccgact acaatactga cgaagccgcc caacgctatc gttttgaaac aaacgg
//
LOCUS       DAOSEX010000026         5215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_14121_length_5215_cov_3.443217, whole genome shotgun sequence.
ACCESSION   DAOSEX010000026 DAOSEX010000000
VERSION     DAOSEX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14121_length_5215_cov_3.443217"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PK612_00955"
     CDS             complement(<1..1214)
                     /locus_tag="PK612_00955"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOH94719.1"
                     /translation="MKKLFKGLFTTALAVGLLAGCKGGASSSEVVTPPSSEDTRAKHS
                     VVLKNYDGTYIDTLQIREGDKATKPADPAEFKPGDDFTFRGWHKEARLQLGEDYEAYD
                     WDTPVTSEVKLLALWEGSDVYVTFYVNELAVIHTEIEVKAGHILLEMPEDPVRDGYEF
                     LGWYKDAALKKPFKPTDFIMEDTDVYPKYEKLYVPDTRTFHVVGSLANPDFSYINWNA
                     VGEEGVDWDVRSYLKKAEDSNLYSIELVIGFRGEFKIKIPNAEWDSELQFSYGKIREE
                     DRNDENFLAGGFDNVQIVNAGAYLIEIETTYEWLKVTRTGDLPEGSPATPNPEEGAID
                     NWGVVGGLNNWGNDGDLDIELTYNDDNLEAPFYYIPFVKLPVGDFELRANNDWGLNFG
                     SHPDNVLPAGFT"
     gene            complement(1404..4295)
                     /gene="pulA"
                     /locus_tag="PK612_00960"
     CDS             complement(1404..4295)
                     /gene="pulA"
                     /locus_tag="PK612_00960"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOH94720.1"
                     /translation="MKKLFTILLASLMVFGSTDKSAPKRAVGPKPTLEVEKVLTPEQA
                     YAEPHIVFHYFRNDANYARYAMWIWGQDEDGSEYNFTETDAYGKYLHVPVANFTDQTQ
                     INFLLKTKGSWEWQTPDVNIQYSDFTFDTVTNAYHFYLVNGLITVFASAEEAKANQVT
                     ESIFASKNTVRVSTNNEPTTFVIKADGTNVTSGAAEHTYNNNTWSYVFTVNLGAAFLP
                     DFSKAYTVEVAFTAGSAVISEIGLSGLFDDPYFIENMTYNGQDLGVTYSPTKSTFKAW
                     APTASELKLRIYESGTPVALNADLGNDTYTEHDFVRTEKGVWSAELAGDYHGKYYTFV
                     AKHPTGYVELIDPYAKSAGINGVRGMIVDFSKTNPTGWDEVDYPVKSHTEIIPYELHV
                     ADLTADESWNGSEENRKRFLGLIEEGTTYTAGGVTVKTGFDHIKELGINALQIIPFYD
                     QANDEREPSFNWGYNPQNYDVLEGSYSSNPYDGLVRIREFKQVVKAYAAEDIRIIMDV
                     VYNHVANLGGHSFNKLVPGYYFRYNADGSPTNASGCGNDTASERIMMENFMRDSTAFW
                     TKEYKIGGFRFDLMGLHTVKSMNTVRNRLVEIRPDIFIIGEAWDMGGGSVSANGANQN
                     NIHKVPGVGAFNDKLRDSVKGDTNGKARGFVQQAASEIYQGTKNSIKDGMLGKINGTQ
                     SSPVQTVNYVACHDNLALYDKLVNAGGHLFGLDQLAQQNIQAEAISLLSQGVSFIHAG
                     SEILRSKPLGDGKFDHNSYQSPYSVNSIKWNEKVTNLNVFERYKELIAIKRDTPGFHY
                     TTSAEISANVTVQFGSELGYQDTLIKLVVVDGETTYTAYFLGAGPRVRINDLEGQYVL
                     FDSTNTMEKWSVLGKSVSLSSNATIVTVSGEGVTPPDSSEEPVTSSSEEPSTPPATST
                     PDLPSSETLPSEEGEDANLTWLYITIPSLAVVGVGVGLILFFRKKKLSV"
     gene            complement(4312..>5215)
                     /locus_tag="PK612_00965"
     CDS             complement(4312..>5215)
                     /locus_tag="PK612_00965"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOH94721.1"
                     /translation="QWMDLRAYAHRYGMQIVGDIPIYVGIDSVDVWTNRKAFLLDEND
                     EPTFVGGVPGDYFNADGQLWGNPLYDWDYLAETGFEFWVDRLRYNARLFDIIRIDHFR
                     GFDSYWKIPAGSSRANDGHWAYPPGYALFDKIYEELPDIKIIAEDLGDLRPEVLELRD
                     HYNLPGMEITSFTFNPFEKRDENAWLENRCIYPGTHDNSPIRGYFKWKDKKFMKAANK
                     LLDDLGFNEFDNFYDNFMAMNFGSDARYAIFQMQDLLRLGDEAKMNSPSTIGSPNWEW
                     KMQDFKTFDEESSLYAYLIEKYNR"
BASE COUNT         1474 a         1093 c          999 g         1649 t
ORIGIN      
        1 tgagtaaatc ctgctggtaa aacgttgtct gggtgtgaac cgaagtttag accccagtcg
       61 ttattcgcac ggagttcgaa gtcaccaact ggaagtttca caaatggaat gtaatagaat
      121 ggtgcttcta aattatcatc attgtatgtt aattcaatgt ctaagtcacc atcattaccc
      181 cagttgttta atccaccgac gacgccccag ttatcgattg caccttcttc aggatttgga
      241 gttgcaggtg aaccttctgg taagtcacca gtacgggtaa ctttaagcca ttcataagtt
      301 gtttcaattt caattaagta ggccccagcg ttaacgattt ggacgttatc aaatccgcca
      361 gcaaggaagt tttcatcgtt tctgtcttct tcgcggattt ttccgtatga gaattgtaat
      421 tcactatccc attctgcatt tggaatcttg attttgaatt caccacggaa tccgataacg
      481 agttcgattg agtataggtt gctgtcttca gcttttttta agtaggaacg gacgtcccaa
      541 tcaacacctt cttcaccaac tgcattccag ttgatatagc tgaagtcagg gttcgctaat
      601 gaaccgacaa cgtggaatgt acgtgtgtct ggtacatata atttttcata ttttggatag
      661 acatcagtat cttccataat gaagtctgtt ggtttaaatg gcttctttaa agcggcatct
      721 ttataccaac caaggaattc gtatccatca cgaacaggat cttctggcat ctcgagtaag
      781 atatgtcctg ctttcacttc aatttctgtg tgaatgacag caagttcatt aacatagaat
      841 gtaacgtaaa catctgaacc ttcccaaagg gctagaagtt taacttcgct ggtaactggg
      901 gtatcccagt cataagcttc ataatcttca ccaagttgta atctagcttc tttatgccag
      961 ccacggaatg tgaagtcatc gcctggcttg aattcagctg gatctgctgg tttagtagcc
     1021 ttatcgcctt ctctaatttg gagggtatca atgtaagtac catcatagtt ttttaagacg
     1081 acactgtgct ttgctctcgt atcttctgat gaaggtgggg taacaacttc tgatgatgat
     1141 gctccgcctt tacatcctgc gagtaagcct acggctaaag cagtggtgaa aagaccttta
     1201 aatagttttt tcattatttt ctccttttct atttttttgc ctaaatggtt tctttggctt
     1261 atttattata caacttttcg cccttaacag tcaagaatat gctaagggct aaagctgttt
     1321 aataacatag ttataatgca taatattcga agtatttgcc taaatgagtg gaaattgccg
     1381 aaatattagt tataaacggc taattatact gataatttct ttttgcggaa gaataatatt
     1441 aagccaacac caacaccaac aacggcgagt gatgggatag taatatagag ccaagtaaga
     1501 ttagcgtctt caccttcttc acttggaagc gtttcactac ttggtaagtc cggagtactt
     1561 gtcgccggcg gagtgcttgg ttcttcgctg cttgaagtaa ctggctcttc gcttgagtca
     1621 ggcggagtaa caccttcgcc agaaacagtc acgattgtgg cattgctact taaggaaaca
     1681 ctcttgccga gcactgacca tttttccatc gtatttgtcg aatcaaagag tacatattgt
     1741 ccttctaaat cgtttatacg aacgcgcgga cctgcaccta ggaagtaagc ggtgtaagta
     1801 gtttcgccat caacgactac tagtttgata agtgtatctt gataccctaa ttctgaacca
     1861 aattggacag tgacatttgc gcttatttcc gcacttgttg tgtaatggaa tccaggagta
     1921 tcacgtttaa tggcaattaa ttctttatag cgttcaaaca catttaaatt ggtaactttt
     1981 tcattccact taatgctatt aacactatat gggctttggt aagagttatg atcaaactta
     2041 ccgtcaccga gtggtttaga acgtaagatt tcactaccag cgtgaataaa ggaaacgcct
     2101 tgtgagagta gtgagatagc ttcagcttgg atattttgtt gcgctagttg gtctagtcca
     2161 aataaatgac caccagcgtt aactaactta tcataaagtg caaggttatc gtggcaggcc
     2221 acatagttta ctgtttgcac tggactactt tgtgtgccat taattttgcc aagcatcccg
     2281 tctttaatac tatttttagt tccttggtaa atttcactag cggcttgttg gacaaaaccg
     2341 cgtgctttac catttgtatc acctttaaca gagtcacgta atttatcatt aaatgctccg
     2401 actcctggaa ctttatgaat gttattttga ttagcgccat tagcacttac actaccgcca
     2461 cccatatccc acgcttcacc gataatgaag atgtcaggac gaatttccac gagacgatta
     2521 cgtactgtat tcattgattt aacagtgtgt aagcccatta agtcaaaacg gaagccaccg
     2581 attttatatt ctttcgtcca gaaagcagtg gaatcacgca tgaagttttc catcatgatg
     2641 cgttcacttg ccgtgtcatt accgcaccca ctagcgtttg ttggtgaacc gtcagcatta
     2701 taacggaaat agtagccagg cactaattta ttaaaggagt gaccaccaag gtttgcaacg
     2761 tgattataaa cgacatccat gataatacga atatcttcgg cagcgtaagc tttaacgact
     2821 tgtttaaatt cacgaatacg cacgagaccg tcatacgggt tacttgagta agaaccttct
     2881 aaaacatcat agttttgtgg gttataaccc cagttaaagg atggttcgcg ttcatcatta
     2941 gcttgatcgt agaacggaat gatttgtagg gcattaattc ctaattcttt aatgtgatca
     3001 aagccggttt tcactgtgac accaccagcg gtgtaagttg tgccttcttc gattaaacca
     3061 aggaagcgtt tgcgattttc ttcgctccca ttccaactct catccgcagt aaggtcggcg
     3121 acatggagtt cgtaaggaat aatttcggtg tgtgatttta ctgggtaatc aacttcatcc
     3181 caccctgtcg gatttgtttt actaaaatca acgatcatcc cgcggacacc attaatccca
     3241 gcacttttag cgtatggatc aattaattca acatagcctg ttgggtgttt agcgacaaaa
     3301 gtgtagtatt tgccgtgata atcacccgca agttcagcgc tccatacacc tttttcagtg
     3361 cgcacgaaat cgtgttctgt atatgtgtca ttaccaagat cagcatttag agcgaccggt
     3421 gtaccacttt cataaatccg taattttaat tcagaagcag ttggtgccca tgctttaaat
     3481 gttgatttag tgggtgaata agtaacccct aagtcttggc cattataagt catgttttca
     3541 ataaaataag gatcatcaaa taatcccgat aaaccaattt cactaatgac agcactgcct
     3601 gcggtaaaag cgacttcgac agtgtaagct ttactaaagt caggaaggaa tgctgcgcct
     3661 aaattaacgg taaacacata tgaccatgtg ttgttattat aagtatgctc tgccgctcca
     3721 cttgtgacat ttgtgccatc agccttaatc acaaaagttg ttggctcatt attagtggaa
     3781 acacgaactg tatttttact tgcaaagata ctttcagtta cttggtttgc ttttgcttct
     3841 tcagcactag caaatacagt aattaaaccg tttactaaat aaaagtgata ggcgttagta
     3901 acggtatcaa atgtaaaatc actatactga atatttacat caggtgtttg ccattcccag
     3961 cttccctttg tttttaagag gaagttaatt tgtgtttgat cggtaaaatt cgccactggc
     4021 acatgtaaat attttccgta tgcatcagtt tcggtgaagt tatattctga tccatcttca
     4081 tcttgacccc aaatccacat cgcataacgc gcataattag cgtcatttct aaaatagtga
     4141 aagacgatat gcggttcagc gtaagcctgc tctggcgtca gtactttttc cacttctaaa
     4201 gtaggttttg gacctactgc tcttttcggt gcacttttat cagtactgcc aaacaccatc
     4261 aaagaggcga gaagtattgt gaaaagtttt ttcataaatt tctctccttt cttaacgatt
     4321 atatttctca attaaatatg cgtataaaga actttcttca tcaaaagtct taaagtcttg
     4381 cattttccat tcccagtttg ggctaccaat tgtacttgga gaattcatct tggcttcatc
     4441 ccctaaacgc aataaatcct gcatttggaa aatagcgtaa cgcgcgtcac taccaaaatt
     4501 catcgccata aagttatcgt agaaattatc aaattcatta aagcctaaat catctaataa
     4561 tttattcgct gctttcataa actttttatc tttccacttg aaatagccgc gaattggcga
     4621 gttatcatgt gtacctggat aaatacaacg attttctaac catgcatttt catcgcgctt
     4681 ttcaaaggga ttaaaagtga aagatgtaat ttccattcct ggtaaattat aatggtcacg
     4741 gagttctaat acttctgggc gtaagtcacc taagtcttca gcgataattt tgatatctgg
     4801 taattcttcg tatattttat caaagagtgc gtatcctggc ggataagccc agtgtccatc
     4861 attagcgcga ctgctaccgg cgggaatttt ccaataggaa tcaaaaccgc ggaagtggtc
     4921 aatgcggata atatcaaaaa gtctagcgtt ataacgcagt ctatcaaccc aaaattcaaa
     4981 gccggtttcc gctaagtagt cccagtcata aagcgggtta ccccacagtt gaccatcagc
     5041 gttaaagtag tcgcctggca caccaccaac gaatgttggc tcatcatttt cgtcaagcaa
     5101 gaatgcttta cgattagtcc aaacgtcaac actatcaata cccacgtaaa tcgggatatc
     5161 gccaactatt tgcatcccgt aacgatgagc gtaagcgcgg agatccatcc attgt
//
LOCUS       DAOSEX010000027         5201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_14174_length_5201_cov_3.482122, whole genome shotgun sequence.
ACCESSION   DAOSEX010000027 DAOSEX010000000
VERSION     DAOSEX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14174_length_5201_cov_3.482122"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PK612_00970"
     CDS             <1..504
                     /locus_tag="PK612_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOH94722.1"
                     /translation="LVKSKVSRITYTKAVEILKEAQQKGVKFQFDNIFWGMDLASEHE
                     RYLCEVYTNGPVFVTDYPKDIKAFYMRLNDDGKTVAACDLLVPGVGELVGGSQREDRY
                     DVLKAKMIEGGNLEDLEWYLDLRKYGGVPHSGYGIGFDRLLMYLTGVQNIRDVQPYAR
                     VFGSIKY"
     gene            514..666
                     /locus_tag="PK612_00975"
     CDS             514..666
                     /locus_tag="PK612_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94723.1"
                     /translation="MNTNKYGQDIPLTKEEIAIRGYKRRNLVLGIIIIILIIIIGIQI
                     GVMLTR"
     gene            complement(656..1375)
                     /locus_tag="PK612_00980"
     CDS             complement(656..1375)
                     /locus_tag="PK612_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94724.1"
                     /translation="MILNPYILIKLSESEKRFLIVLFFLIVVVLFVCGLFYEGITRFM
                     ARQGEDIGRLMATQVESGYISSLKQFKRNSYRKSRLEFYRQFIKTFIMMIIWFAIYGT
                     VSIFYRHWLDLFDYTSEGFTTLLWKFDYANTPRTTFFGMSIISDWPPLLHKPFFSAKA
                     IPSYLLAVFGIVIIILFVAQVLSYLARTIFIIQHSKKMFTRDLRDYKLSDLSAAPAEL
                     QESGVNIPEVKPKEPLPPTNG"
     gene            complement(1372..1797)
                     /locus_tag="PK612_00985"
     CDS             complement(1372..1797)
                     /locus_tag="PK612_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94725.1"
                     /translation="MKGLFKRLGQGILFILALPFGIAIFILFSIYAIITFFYTFFSAI
                     PAFFKGENVFGPTELDIAASTRLAEQKLAAQQPPPPPVVQPSTTINLFTVQQPGTEQV
                     PPPQVTYTQSDGVTYRRVTEQEVAALPEETIIDNSEEQK"
     gene            complement(1781..2692)
                     /locus_tag="PK612_00990"
     CDS             complement(1781..2692)
                     /locus_tag="PK612_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94726.1"
                     /translation="MTMKMNSLSRTHQLVLGALMGAINVIFALISSYLFAFSLIIMLF
                     LPLASIIVAINIDLKFYPVYLLGTLTLALVLNLGNIDNTLFFLLPILTSGLAFGLLIR
                     HKVPDILILLIVSGVNFLTLLITIPIINLIYDVNFLQVFASFIGFNNIEFGELVLPSI
                     LTLLAVMQTLITLVIVTQDAAYFRLEINTEEWPYISLVNLGFSAIVTVLMFFNHGISL
                     ALLFVVILLSLYQIVHLFQKHTIFAWSTLLATIVFIIIGLALFENYTSLPYYFGIIIA
                     VIPVVISDILWLYISRKKSEAKNEGTI"
     gene            2762..4963
                     /locus_tag="PK612_00995"
     CDS             2762..4963
                     /locus_tag="PK612_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH94727.1"
                     /translation="MNLYENLNSKQLEAVTTSGQYVRVIAGAGSGKTRVLTTRIAYLV
                     QEIGTPEDRIVGFTFTNKAAGEMTNRLKKYLERDNVRVRLSTFHSFGARLLREDIDVL
                     GYSRSFTIYDEEDTSSLIRNISVERGHERRGELTRDAINFIGAVKEKGLDAFTYKIRP
                     HGFVNEKEFVEIWKEYERRLKASNCLDFSDLISKSITILEEYRAIREKWQHRFDYILV
                     DEFQDTNDLQYKLIKLLMHQNTSLYVVGDPDQTIYTWRGANERIIMNFDSDFKNAETI
                     ILEENYRSTKYILDAANKLIAHNKNRVEKNLFTAKEKGEPVIVQYTESDSREVNWIYE
                     QIISLRLNVKDFSFRDVAILMRANYLTLPFERHFLHKNVPYRIYGGVRFYQRREIKDV
                     LAYLRILVNEKDDISFSRIVNVPRRGMGNVAMENLKIAALKNEYSLFETVRYVEDIGL
                     SSASQEQLKALMNAVDETRTKLQNDKLELSTVINDYIQKVGYYEHIKTEKDEDKQADM
                     RANVQVLIADIKQFMNDYPESTFNDYLENASLHSAQDEVEEGDYITLMTVHMAKGLEY
                     DYIFVSGLVEGVFPNNRALLDAGRAGEEEERRLCYVAFTRARKRLYLTMSSQYNFAIA
                     SGGRPSRFISEAGLGPPKIKEYHPKYGTPLKEGGVKQVKKEAPRYRANYAEYDYASDF
                     PYAVNDIVEHKTFGSGIVTAIFPTQTTIEVKFDTGEVKRLIASPKYFKEKK"
     gene            4966..>5201
                     /gene="ligA"
                     /locus_tag="PK612_01000"
     CDS             4966..>5201
                     /gene="ligA"
                     /locus_tag="PK612_01000"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006061236.1"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOH94728.1"
                     /translation="MDYKARILYLREVLNKYNYEYYVLNNSSVSDAEYDSLMQELIAL
                     EKEHPDMFDKTSPSQRVGGFVASEFNKITHSSMML"
BASE COUNT         1828 a          818 c         1008 g         1547 t
ORIGIN      
        1 ttagtgaaaa gtaaagtatc acgtattaca tacactaaag ctgttgaaat attaaaagaa
       61 gctcagcaaa aaggcgttaa attccaattt gacaatattt tctggggtat ggatttagca
      121 agtgaacatg aacgttatct ttgtgaagtt tatacgaatg gtccagtgtt tgttactgat
      181 tatcctaaag acattaaagc attttatatg cgcctaaatg atgatggtaa aacagtcgca
      241 gcgtgtgact tattagtgcc aggtgttggt gaactagttg gtggttcaca aagagaagat
      301 agatacgatg ttttaaaagc aaagatgatt gaaggcggta accttgaaga cttagaatgg
      361 tatttagact tacgtaaata tggtggtgtc ccccattctg gatacggtat tgggtttgac
      421 cgcttattga tgtacttaac tggagtgcaa aatattcgtg atgttcaacc ttacgctcgg
      481 gtatttggct caattaaata ttagaggtgc aaagtgaata caaataaata cggacaagat
      541 atccccttaa caaaagaaga aatagcaatc cgtggttata aaagacggaa cctcgtttta
      601 ggtatcatta ttattatttt aataattatt attggaattc aaattggtgt aatgcttacc
      661 cgttagtcgg tggtaagggc tctttaggtt taacttcagg aatattcaca ccgctttctt
      721 gtaactcagc aggagcggca cttaaatcac ttaatttata atcacgtaaa tcgcgagtaa
      781 acatcttttt actatgttga ataataaaga tcgtacgcgc taagtaggaa agtacttggg
      841 caacaaataa aatgataatc acaataccga acaccgctaa aaggtagctt ggaattgctt
      901 tagcgctgaa gaatggttta tgaagaagag gtggccagtc actaataatg ctcatgccaa
      961 agaaagtagt gcgtggcgta ttagcgtaat caaatttcca taagagggta gtaaaacctt
     1021 cactagtgta atcaaataaa tctaaccagt gacggtaaaa gatactaaca gtaccataaa
     1081 tcgcaaacca gataatcatc ataatgaatg tttttataaa ttgacgataa aattctaagc
     1141 gtgatttacg gtaagagttt cttttaaatt gtttaagtga agatatataa cccgattcca
     1201 cctgtgttgc catgagtctg ccaatatctt caccttggcg tgccataaag cgcgtaatac
     1261 cttcataaaa tagtccgcat acaaaaagca caacgacaat taggaaaaag agaacgatta
     1321 aaaacctttt ttcactttca ctaagcttaa ttaatatata cggatttaaa atcatttttg
     1381 ctcctcgcta ttatcaataa tagtttcttc aggaagtgct gcaacttctt gttcagtaac
     1441 acggcgataa gtaacaccat cactttgggt ataagtgact tgtggtggtg gcacttgttc
     1501 ggtgccgggt tgttgtactg taaataagtt aatagttgtt gaaggttgca cgactggtgg
     1561 cggtggtggt tgttgcgccg ctagtttttg ttcggctaag cgtgtactag cggcaatatc
     1621 taattcggtt ggaccgaaga cattttcacc tttaaagaat gctggaatag cagaaaagaa
     1681 agtatagaaa aaagtaataa tggcgtaaat tgaaaaaagg ataaagatcg ctatcccaaa
     1741 tggtagcgct aggataaaaa gaattccttg acctaatctt ttaaatagtc ccttcatttt
     1801 ttgcctcgct tttctttcta ctaatatata accacaaaat gtcgctaatc acaactggaa
     1861 ttaccgcaat aataatacca aaataataag gaagcgaagt ataattttca aataatgcta
     1921 aaccaataat aataaacaca attgtcgcaa gtaatgttga ccatgcaaaa atggtgtgtt
     1981 tttggaaaag atgcacgatt tgatagagtg aaagcaaaat aacaacgaac aaaagcgcaa
     2041 gcgaaatccc gtgattaaaa aacattaata ccgtaacaat agcgctaaat cctaaattaa
     2101 ctaaactaat ataaggccac tcttccgtat taatttctaa tctaaaataa gcagcgtctt
     2161 gcgttacaat cacaagcgta atgagtgttt gcataaccgc taataaggtt aagattgaag
     2221 gcaacactaa ttcaccaaat tcaatattat taaaaccgat aaaactagcg aacacttgta
     2281 agaaattcac atcataaatt aaattaataa tcggtattgt aattaaaagt gtaagaaagt
     2341 tcacaccgct aacgattaat aaaattaaga tgtcgggtac tttgtgtcta attaaaagcc
     2401 caaaggctaa tccgcttgtt aatatcggta ataagaaaaa aagtgtgtta tcaatattac
     2461 caagatttaa tacaagcgct aaggtaagcg tacctagtaa ataaacagga taaaatttta
     2521 aatcaatatt aattgcgaca ataatactcg ccagcggtaa aaaaagcata ataattaaac
     2581 taaaagcaaa taaataactg ctgataagtg caaatattac atttatcgca cccatgaggg
     2641 cgcctaaaac gagttgatgt gtgcgtgata atgagttcat tttcatagtc atattttaac
     2701 ataaaaaagg gcttcactac gggagtaatt tcaccttata ttatgttata atattttaga
     2761 tatgaatttg tatgaaaatc ttaattcaaa acagttagaa gctgttacta caagcgggca
     2821 atatgtgcgt gttattgctg gtgcgggaag tggtaaaacg cgagtactca ctactcgtat
     2881 tgcttattta gtgcaagaaa taggtactcc ggaagaccgc attgtcggtt ttacttttac
     2941 aaataaagcc gctggcgaaa tgacaaatcg cttaaaaaag tatttagaac gcgataatgt
     3001 tcgtgtgcgt ctttcaacat ttcactcttt tggtgcaaga ctactgcgtg aagacattga
     3061 tgttttaggc tattcacgca gctttacaat atatgacgaa gaagatacaa gttccttaat
     3121 tcgcaatata agtgtagaaa gaggacatga acgccgcggc gaattaacgc gtgacgcaat
     3181 taattttatt ggggcggtta aagaaaaagg cttagatgct tttacttata aaattagacc
     3241 gcacggcttt gttaatgaaa aagaattcgt tgaaatatgg aaagaatatg aacgaagact
     3301 taaagcgtca aactgtcttg attttagtga tctaattagt aaatcaatta cgattttaga
     3361 ggaatataga gcaattagag aaaaatggca acatcgcttt gattatattc tcgttgatga
     3421 atttcaagac actaatgatt tgcaatataa actcattaaa cttttaatgc accaaaatac
     3481 atcactctat gtcgtgggtg atcccgatca aacaatttat acatggcgtg gcgctaacga
     3541 gcgaatcatt atgaattttg atagtgattt taaaaatgcc gaaacgatta ttctagaaga
     3601 aaactatcgt tccactaaat atattttgga tgcagcaaat aaacttattg ctcataacaa
     3661 aaatagagtg gagaaaaatc tttttaccgc taaagaaaaa ggtgaaccag tgattgttca
     3721 atatacagaa agcgattcac gcgaagttaa ttggatttat gaacaaatta tttcattgcg
     3781 cttaaacgtc aaagattttt cgtttcgtga tgtcgcaatt cttatgcgcg ctaattactt
     3841 aacactacca tttgaacggc actttttaca taagaatgtt ccgtaccgta tttatggtgg
     3901 cgtgcgtttc tatcaaagac gcgagattaa agatgtttta gcgtatctgc ggattttagt
     3961 taatgaaaaa gatgatattt cttttagcag aatcgttaat gtcccgcgcc gtggcatggg
     4021 taatgtagcg atggaaaacc ttaaaattgc tgcgttaaag aatgaatatt cactatttga
     4081 aacagtgcgt tatgttgaag atattggctt atcaagtgct tcacaagaac aattaaaagc
     4141 gttaatgaat gctgtggatg agacacgaac aaaattacaa aatgacaaac ttgaactaag
     4201 tactgttatt aatgactata ttcaaaaagt tggttactac gaacatatta aaaccgaaaa
     4261 agatgaagat aaacaagcag atatgcgtgc taacgtccaa gtcttaattg ccgacatcaa
     4321 acaatttatg aatgattatc ctgaatcaac ctttaatgat tatttagaga acgctagttt
     4381 acatagcgcc caagatgaag ttgaagaagg tgattatatt acgcttatga ctgtgcatat
     4441 ggcaaaaggt cttgagtatg attatatttt tgtttcagga cttgttgaag gtgtctttcc
     4501 aaataaccga gcgttgcttg atgctggtcg cgctggtgaa gaggaagaaa gaagactatg
     4561 ttacgttgct tttactagag cgcgtaaacg gctttattta acaatgagtt cgcaatataa
     4621 ctttgcgatt gcttccggcg ggagaccatc acgctttatt agtgaagcgg ggttaggacc
     4681 accgaagatt aaagaatatc atccaaaata cgggacaccg ctcaaagaag gtggtgttaa
     4741 acaagttaaa aaagaagcgc cgcgttatcg cgctaattat gcagaatacg attacgctag
     4801 tgattttcct tatgcagtta acgatattgt ggaacataaa acatttggat caggaattgt
     4861 tactgccatt tttccaacgc aaacaacaat tgaagttaag tttgatactg gcgaagtaaa
     4921 gcgactaatc gctagcccaa aatactttaa ggagaaaaaa taaagatgga ttataaagca
     4981 agaattcttt atttacgcga agttttaaat aagtataact atgaatatta tgtccttaat
     5041 aattcgagtg ttagtgatgc tgaatatgat agtttaatgc aagaattaat tgcccttgag
     5101 aaagaacacc ctgacatgtt tgataaaaca agtccaagtc aacgagtagg cggttttgtg
     5161 gcgagtgaat ttaataaaat tacccatagt tcaatgatgc t
//
LOCUS       DAOSEX010000028         5071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_14673_length_5071_cov_3.196770, whole genome shotgun sequence.
ACCESSION   DAOSEX010000028 DAOSEX010000000
VERSION     DAOSEX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14673_length_5071_cov_3.196770"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..415)
                     /gene="rpsM"
                     /locus_tag="PK612_01005"
     CDS             complement(38..415)
                     /gene="rpsM"
                     /locus_tag="PK612_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOH94729.1"
                     /translation="MARIVGVDIPNDKRVVVSLQYIYGIGLTTAKKILAAVKIDENVR
                     VKDLTEEELGAIRQEITKYRVEGDARRQVALNIKRLTEIGSYRGIRHRRGLPVRGQRT
                     KTNARTRKGPKKTIANKKQASQG"
     gene            complement(427..540)
                     /gene="rpmJ"
                     /locus_tag="PK612_01010"
     CDS             complement(427..540)
                     /gene="rpmJ"
                     /locus_tag="PK612_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOH94730.1"
                     /translation="MKVRASVKPICDKCRVIRRKGNVMVICENPKHKQRQG"
     gene            complement(563..781)
                     /gene="infA"
                     /locus_tag="PK612_01015"
     CDS             complement(563..781)
                     /gene="infA"
                     /locus_tag="PK612_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOH94731.1"
                     /translation="MSKSNVITVEAKVIECLPGVNFRVQLDNGHIILAHVSGKIRRYN
                     IRILPGDRVAVEISPYDLTRGRITYRYR"
     gene            complement(781..1539)
                     /gene="map"
                     /locus_tag="PK612_01020"
     CDS             complement(781..1539)
                     /gene="map"
                     /locus_tag="PK612_01020"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOH94732.1"
                     /translation="MISQKSPREVRLMKESGRIINLVFKEVEAKLRPGMSTADVDKLA
                     LEVITREGGTPTFKGYGGFSGNICVSVNDTLIHGIPSHREIIKDGDIVSVDVGVTKDG
                     YVADAARTFLVGNVSENAKRLVEVTEESFWYAVDNFAKPGNYIGDISHAIYEYASSRG
                     YTLTRDYTGHGVGRKLHEAPAVPNVGTPKTGPRLKVGMTLAIEPMLNEGLVDLIVLKD
                     GWTVKTKDGKLASHYENTIVITEDGYEVLTMEEK"
     gene            complement(1532..2191)
                     /locus_tag="PK612_01025"
     CDS             complement(1532..2191)
                     /locus_tag="PK612_01025"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOH94733.1"
                     /translation="MCNIILLGPPGAGKGTLAKQLISVYNIPHISTGDMFREAIKEGT
                     PLGRLAASYINEGHLVPDEVTISLVRERLSQEDCANGYLLDGFPRTLPQAEALDTIGK
                     DLGRPINVVINITADETELVRRISGRRVCRKCGAPYHIDTMKPKKDGVCDACGGELYQ
                     RKDDNKDNLLVRLNHYYADTKPLEDFYAEHGVLYAVDSTAGAKKSLKAAQKIIEGAKC
                     D"
     gene            complement(2196..3542)
                     /gene="secY"
                     /locus_tag="PK612_01030"
     CDS             complement(2196..3542)
                     /gene="secY"
                     /locus_tag="PK612_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOH94734.1"
                     /translation="MKKIAAIFKNKEIVDRIFFTIMIMFVFRIGAAITVPGVTLSDFS
                     FQNNDAFGLMNLLGGGILQQFSVFALGVSPYITASIIVQLLSMDVLPALTDLANQGDY
                     GRKKMEMTNRYLTLMLGAVQAYGVIVTMENQSYITLKESNFWTYAYIITVMLAGTMLV
                     MWLGEQISSKGIGNGISMIIFAGIVSSLPQQIIRAYQNWLSDKVGQGSQQLFIGLLQF
                     GVYVLSFLAIIGFITFIETSKRKIPVQHAGKSGQTRKMAQASFLPIKINSAGVIPVIF
                     ASSIMMAPSIIASFISGSNLDAEWLKIFNSSATVTMPWFNDKTWQMPWGLLIYLFLIV
                     VFAFFYAKLQINPEKLAENFQKNGSYIPGVRPGAETERYVSKVLNRITVIGAFSLAVI
                     AALPIILVLLKVVPDQSLALGGTGLIIVVGVAIEINNQIDGLLAGKSYDEGSVLGG"
     gene            complement(3544..3984)
                     /gene="rplO"
                     /locus_tag="PK612_01035"
     CDS             complement(3544..3984)
                     /gene="rplO"
                     /locus_tag="PK612_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOH94735.1"
                     /translation="MKLHELTYTEGAKRARKRLGRGIGSGTGKTSGKGEKGQKSRSSG
                     GTRLGFEGGQMPLYRRIPKRGFTNMNTRKFAVVNLEELNVFEDGTVVTPTLLIEKGII
                     KQELGGVKVLARGELTKKLTVKAHRFSKQASDAIAKLGGTAEVI"
     gene            complement(3995..4639)
                     /gene="rpsE"
                     /locus_tag="PK612_01040"
     CDS             complement(3995..4639)
                     /gene="rpsE"
                     /locus_tag="PK612_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOH94736.1"
                     /translation="MDKEKKNLVEDTEEVETEEVETEEKDTEQTPQGDAPRRSNRGGR
                     REGGPRRGVRRAPARRPKEFEERVVAINRVSKTTKGGRRLRFTALVVIGYGKGKYGYG
                     NAKAAEVPDAIRKAIEVAKKNTFTVELVKGDTIAHEIVGRFGATRVFLKPAPEGTGVI
                     AGGSVRAILELAGIRNVYSKVYGSRTALNVLKATDNGLRGLKRASDIKRLRGTL"
     gene            complement(4653..5009)
                     /gene="rplR"
                     /locus_tag="PK612_01045"
     CDS             complement(4653..5009)
                     /gene="rplR"
                     /locus_tag="PK612_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOH94737.1"
                     /translation="MVKQIKRNEIRKRRHARIRRKISGTHEVPRLVVFRSNKHIAVQL
                     IDDTKGVTLGSASSLAMKLVHGDNVESAAKVGTEIANVAKNLGITKVVFDRGGYLYHG
                     RIKALADAARKAGLEF"
BASE COUNT         1302 a         1210 c          983 g         1576 t
ORIGIN      
        1 cgtgcttttt ttgacataac taactttcac tccttcctta accttgtgac gcttgctttt
       61 tattagcaat cgttttcttc ggtcctttac gtgttcttgc atttgttttt gtgcgttgac
      121 cacgaactgg taatccgcga cgatgacgaa tcccgcggta tgaaccgatt tctgttaatc
      181 gtttaatatt taatgctact tgccgtctgg cgtcaccttc gacgcggtac ttcgtaattt
      241 cttgacgaat tgcaccaagt tcttcttcgg ttaaatcttt aacacgaaca ttttcatcaa
      301 ttttaacagc ggctaatatt tttttagcgg ttgttaagcc aataccataa atgtattgta
      361 agctcactac gacccgctta tcgtttggaa tatctacacc aacaatccgt gccatattct
      421 ttcctcctat ccttgtcttt gcttatgctt agggttttcg caaatgacca tcacattgcc
      481 tttgcggcga ataacccggc atttatcgca aattggtttg actgaggctc ttactttcat
      541 aatgaatttc ctccaatttc ttttatcgat accgatatgt aatgcggccc cgtgttaaat
      601 catagggcga aatttcaact gcgacacgat cacctggtag aatgcgaatg ttataccttc
      661 tgattttacc agaaacgtgg gccaaaataa tgtgtccgtt gtctaattga acgcggaaat
      721 taactcccgg taaacattca atgactttcg cctcgacggt aattacgttc gatttactca
      781 ttacttctcc tccatagtta atacttcgta gccatcttcg gtaataacga tggtgttttc
      841 ataatggctc gctaatttac catcttttgt tttgacggtc cagccgtctt ttaagacgat
      901 aaggtcgacc aacccttcgt ttaacattgg ttcaatcgct aaggtcatac ccacttttaa
      961 gcggggacca gtctttggcg ttccaacgtt tggtacagcg ggtgcttcat gtaatttacg
     1021 ccccacaccg tgacccgtgt aatctcgcgt aagtgtatac ccacgacttg acgcatattc
     1081 ataaattgcg tgactgatat ctccgatgta gttccctggt tttgcaaagt tgtcgactgc
     1141 ataccagaag ctctcttcgg taacttcgac gagtcttttt gcgttttcgc taacatttcc
     1201 gactaagaat gtccgtgctg catccgcaac atacccgtct tttgtcaccc cgacgtcaac
     1261 actcacaatg tcgccgtctt taataatttc gcggtggctt gggattccgt ggattaatgt
     1321 atcattaacc gaaacacaga tattgccact aaatccgcca taacccttga aggttggggt
     1381 gccaccttcc cgagtaatga cttcaagcgc tagtttatca acatcagcag ttgacatccc
     1441 aggtcttaac ttagcctcta cttctttaaa gactaagtta ataatccgac ctgattcttt
     1501 cattaagcgc acttcgcggg gtgatttttg actaatcaca tttagctcct tcaataattt
     1561 tttgtgcagc ttttagtgat tttttcgccc cagcagtact atcaacagcg tagaggacac
     1621 catgctctgc atagaagtct tcaagtggct ttgtatcagc gtaatagtga tttaagcgca
     1681 ctaagagatt gtctttattg tcatctttgc gttgatataa ttcaccacca caagcgtcac
     1741 acaccccgtc ttttttcggc ttcatcgtat caatgtggta tggagcgccg catttacgac
     1801 atactctgcg cccactgatg cgccgcacta attcggtttc atcagcagta atattaatta
     1861 ctacattaat tggccgacct aagtctttgc caattgtgtc gagtgcttcg gcttggggga
     1921 gggttcgtgg gaacccgtct aacaagtaac catttgcgca atcttcttgc gataagcgtt
     1981 ctcttacgag actaattgtt acttcatctg gcactaagtg cccttcattt atataacttg
     2041 ccgcaagtct gccaagtgga gtgccctctt taattgcttc acggaacatg tcacctgtag
     2101 agatatgcgg aatgttatag acactaatta gttgtttggc aagtgttcct ttaccagccc
     2161 ctgggggtcc taataagata atattacaca tacgcctaac ctcctaatac gcttccttcg
     2221 tcataagact taccagcaag gagcccatca atttggttat taatttcaat tgccaccccg
     2281 acgacaatga ttagccctgt tccgcctaat gctaagcttt ggtcaggaac gactttaagt
     2341 aagactaaga taatcggtaa ggccgcaata accgctaaac tgaaggcacc aatgacggta
     2401 atacggttaa gtactttaga cacatagcgt tccgtttcag cacccggacg aacaccagga
     2461 atataactac cattcttttg gaagttttcc gcgagctttt ccggattaat ttgtagttta
     2521 gcgtagaaga aggcaaacac aacaataagg aataagtaaa tgagtaatcc ccacggcatt
     2581 tgccaagtct tatcattaaa ccacggcatt gttactgtcg cgcttgagtt aaagattttt
     2641 aaccattcgg cgtctaaatt tgagccgctg ataaaagaag caatgattga cggcgccatc
     2701 ataatacttg aagcaaagat gacagggata accccggcac tattaatctt aattggtaag
     2761 aaacttgctt gcgccatctt gcgggtttgc ccgcttttac ccgcgtgttg cacgggaatt
     2821 ttacgtttac tcgtttcaat aaaggtaata aaaccaataa tggcgaggaa actaagtaca
     2881 taaactccaa attggaggag accgataaat aattgctgac tcccttgtcc aactttatca
     2941 ctaagccaat tttggtaggc acgaataatt tgttggggga ggctgctaac gatacctgcg
     3001 aagataatca tcgagatacc attaccaatc cctttactac taatttgctc accaagccac
     3061 attacaagca tcgtgcccgc aagcatgacg gtaataatgt aagcatatgt ccaaaagttt
     3121 gattctttca gcgtaatata actttgattt tccatcgtaa caataacgcc ataagcttgc
     3181 actgcgccga gcatcagcgt taagtaacga tttgtcattt ccattttctt ccgcccataa
     3241 tccccttgat tagcaaggtc agtaagcgct ggaaggacgt ccattgataa gagttggaca
     3301 ataattgatg cggtaatata aggactaacc cccagtgcaa acacactaaa ttgttgtagt
     3361 atgcccccac caaggaggtt cataagccca aaagcatcat tgttttggaa tgaaaagtcg
     3421 ctaagcgtga cgcctggaac cgtaatcgcg gcgccaattc ggaagacaaa cataatcatg
     3481 atcgtaaaga agatacgatc gacaatttct ttattcttga aaatcgcggc gatctttttc
     3541 atattaaatt acctctgcgg ttccacctag tttagcaatt gcgtcactag cttgtttgga
     3601 aaatctgtgg gctttaactg ttaatttctt tgttaattct ccgcgcgcta agactttaac
     3661 gccaccgagt tcttgtttaa tgatcccttt ttcaattaag agtgttgggg tcacaacagt
     3721 tccgtcttca aagacattta attcttctaa attaacgacg gcaaactttc gtgtattcat
     3781 gtttgtaaag ccacgtttgg gaatacggcg atataaaggc atttgtccac cttcaaagcc
     3841 gagacgcgtg ccaccgcttg agcgtgactt ttgacctttt tcaccttttc cacttgtttt
     3901 gcctgtgcca gagccaatcc cccggcctag acgtttacga gctctttttg ctccttctgt
     3961 ataagtaagt tcgtgtaatt tcatactcta cctcctacaa tgttccccgg agacgtttaa
     4021 tatccgaagc tcgttttaaa ccgcgtaatc cgttatctgt tgctttaagc acattaaggg
     4081 cggtacgcga accataaact tttgaataga cgttgcgaat acctgcaagt tctaagatgg
     4141 cccgcactga accaccggcg ataactccag taccttctgg tgctggtttt aagaatacgc
     4201 gggttgcacc gaagcgacca acgatttcgt gggcaattgt gtcaccctta acaagttcaa
     4261 cagtgaaggt gtttttcttt gcaacttcaa ttgctttacg aatcgcatca ggtacttctg
     4321 ctgctttagc gtttccgtag ccatacttcc ctttaccata gccaataacg actaaggcag
     4381 taaagcgtaa gcgacggcca ccttttgttg ttttactaac gcggttaatc gcgacgacgc
     4441 gttcttcaaa ttcttttggg cgacgcgctg gggcacgacg gacaccacga cgcggaccac
     4501 cttcacgacg gccacctcta ttactacggc gcggggcatc accttgcgga gtttgttcgg
     4561 tatctttttc ttcagtttct acttcttcag tttcaacttc ttcagtatct tctactaagt
     4621 ttttcttttc tttatccata actttactcc ttctagaatt ctaacccggc cttacgggca
     4681 gcatcggcta aggctttgat tcgaccatgg tataagtacc ctccgcgatc gaacacaacc
     4741 ttggtaatgc ctaagttttt tgcaacatta gcaatttcag taccgacttt tgccgctgat
     4801 tcaacattgt cgccatgcac taatttcatc gctaatgaac tagcgctacc taaggtaaca
     4861 cctttagtgt catcaattaa ttgcacagca atgtgtttgt tggaacggaa gacgacaagg
     4921 cgcggaactt cgtgtgtacc gctaattttg cggcgaatac gggcatgacg ccgttttcta
     4981 atttcattac gtttaatttg tttaaccata acttttcacc tatttcttgg cgcctgcgcg
     5041 ctttccttcc ttacgaataa taacttcgtc g
//
LOCUS       DAOSEX010000029         4975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_15050_length_4975_cov_3.297358, whole genome shotgun sequence.
ACCESSION   DAOSEX010000029 DAOSEX010000000
VERSION     DAOSEX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15050_length_4975_cov_3.297358"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PK612_01050"
     CDS             complement(<1..1068)
                     /locus_tag="PK612_01050"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOH94738.1"
                     /translation="MKFDNNPFTLSDKTIAIPNDTILERTVLGLMLTSAEALEVGMQH
                     LEASYFPNIDNNNRAIFYAMKLLHEENVKVDIVTVSGKLRELRLFSAVGGLEYLSLIA
                     EEAITYSAIKDYCLGLRDLDLLRKALKVMDQGVEKHRQGQVGDVNDYLSDLLRDITKV
                     AEERRIMDFESLGDIAKGLKAHLEKMRKSSGNKLTGNSTGFYDIDRYTHGFQEDNYII
                     IAARPAVGKTAFGLSLAYNMAKINNKTVGFFSLEMGNQQLMQRLVSKVSGVLHKNIMT
                     GYLSKEERVKVYETIENLKHIKLYIDETPSININDLLIKARKLKRENDDLCAIFVDYI
                     GLITTTGHVESRQIELSEISAA"
     gene            complement(1071..1520)
                     /gene="rplI"
                     /locus_tag="PK612_01055"
     CDS             complement(1071..1520)
                     /gene="rplI"
                     /locus_tag="PK612_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOH94739.1"
                     /translation="MKVILLKDVKKVGKKDEIVDVSDGYATNFLIPRKLAVPYNERTI
                     SQLKANQARALAEEKAHKEEALVNKKKLEEKEISFILAIGEKGKAFGAITAKKVEEKI
                     KEEFKINVDRRKFVNFQPLNQLGPTIVEIELFKDVIAKVKVLVKDKE"
     gene            complement(1522..3609)
                     /locus_tag="PK612_01060"
     CDS             complement(1522..3609)
                     /locus_tag="PK612_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH94740.1"
                     /translation="MIKAITKLRKRTIILVIVEGLLLGLISLFYYLNLFNFQEIFVPP
                     VIFSVAIGVVFFNLFVFIFITARINKLRKKNTFELVNLLGGDVKEAYNFGMLGLVVVD
                     NDFNVTWASELFKERRLLIIDKNILAWKPELIWLVELSEVLADNVEDTIKVEIESRNY
                     EVKYLSKAGIFLFKDITEFEYLFDSNQATAPVLGVVVIDNFVDIVVKGEHSNPIFGTV
                     NSLILEYFSNYGVLLKQYREDAYLLILNKENFDKLYADNFSILQKIKDKTSNQDYQIS
                     LSMAFAYNFPDVLKLNELVLNAIDTAMARGGDQVVIARYGEESEFIGGATSQAEKRNK
                     TAIRLYADTIFRHIREAKNVLIMTHTDTDFDALGASLGLKAMADYVKKTQKGTKVQFQ
                     PARIVYEHNLTELKTRMAVNKMFTKQEMNDMFISPEEIDDQKGTASLVNSGTLLIIAD
                     VSCPQLTLHPKLLERVDKVIVIDHHRRTDEFIDQRLYDYIETSASSTCEIVTELLHYG
                     NTPNFVLPPKYATFMLAGIYLDTNYFRTKTTGSRTFLASMVLEDWGADVMTTDDFLKD
                     NVEEYALITKIMNSMTTLSVGIVFLRADETDIVTPETLSKVANQSMRIKGIQASFVIG
                     YTAPKVVKVSGRSNGEINVQFLLEKVGGGGHQASAAAVFTDKTLDDVQEILEDVINQN
                     LSQAKVVGGKKGG"
     gene            complement(3731..4327)
                     /locus_tag="PK612_01065"
     CDS             complement(3731..4327)
                     /locus_tag="PK612_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94741.1"
                     /translation="MARKKKNDVALYDDSSYEEDDLKYAADEEVYAESLEDDEGKAKK
                     KRKKKKKEPKDPKERKDGSETRGGKTLTDRYHYRGRYYLLPERELRKQLRFYQKVFNY
                     RFLGIIAGVIALIFSILSLTFNAYFAVFTLLIVITGLIYGLLGMLKLRGLNWLFVLIG
                     IGLNIMAIVLAMKPLTFVFKHLGEIMALINEYMSSAGS"
     gene            complement(4409..>4975)
                     /locus_tag="PK612_01070"
     CDS             complement(4409..>4975)
                     /locus_tag="PK612_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94742.1"
                     /translation="VQKDPHFAYDIQFGQYLELFIKLFKIDETSNYAQNVGVIIKNGV
                     IAYTIVFSFISVLLTHILIHLTRKFICREKMEHGPLHGLEFTISRVAAITYLVGCVVV
                     LVLSFLLTKELNLLGISSINAIFTVFFSVTIIFVFQGILAVNLRLEAKGCNNLSTLVF
                     ISGILLSPVFAIIGAINPLLPRKEKAVV"
BASE COUNT         1567 a          937 c          761 g         1710 t
ORIGIN      
        1 ggccgcactt atttcgctaa gttcaatttg cctactttca acatgtcccg ttgttgtaat
       61 taaaccgata tagtcaacaa aaatagcgca taaatcatca ttttcacgtt ttaatttccg
      121 cgctttgata agtaagtcat taatatttat acttggtgtt tcatcaatat aaagtttaat
      181 atgctttaag ttttcgattg tttcgtaaac tttaacgcgt tcttctttac taagatagcc
      241 tgtcataata tttttatgaa gcacacctga aactttagaa acaagccgtt gcattagttg
      301 ttggttgccc atttcaagtg agaaaaagcc aaccgtttta ttatttattt tagccatgtt
      361 ataggctaaa cttagtccaa atgctgtttt accgaccgca ggccgtgcgg caataataat
      421 atagttatct tcttggaacc cgtgagtgta gcgatcaata tcataaaagc cagtcgaatt
      481 tcctgtgagt ttattaccag aagatttccg cattttttca aggtgagctt ttaacccttt
      541 agcgatatca ccaagtgatt caaagtccat aatccgtcgc tcttccgcaa ctttagtaat
      601 gtcgcgtaat aaatcactta agtaatcatt aacatcacca acttgacctt gacggtgttt
      661 ttcaacgcct tgatccatta cttttaaggc tttacgaagt aaatctaaat cacgtagtcc
      721 taagcaataa tcttttatcg ctgaatatgt aatcgcttct tcggcaatta aacttaagta
      781 ttctaaacca ccaacagcgc taaatagtcg taattctctt agtttaccgc tgacggtaac
      841 aatatcaact ttgacatttt cttcatgtaa taatttcatt gcataaaaaa tagcgcggtt
      901 attattatcg atgtttggaa aataacttgc ttctaagtgc tgcatcccta cttctagtgc
      961 ttcagcactt gttagcataa gacctaacac tgttctttct agaatagtgt cattaggaat
     1021 cgctattgtt ttatccgata gtgtaaaagg attattatca aatttcataa ctactcctta
     1081 tctttgacta gaacttttac tttagcaatg acatctttaa aaagttcaat ttccactatt
     1141 gtgggtccta attgatttag tggctgaaag ttcacaaact tacggcgatc aacattaatt
     1201 ttaaattctt ctttaatctt ttcttcaact tttttagcgg taatcgcccc aaaagcttta
     1261 cctttttcac caatagcgag aataaaacta atttcttttt cttctagttt tttcttgttg
     1321 acaagcgctt cttctttatg tgctttttct tcggctaaag cacgcgcttg atttgctttt
     1381 aattgggaaa tagtccgttc attatacgga acagcaagtt tgcgtgggat taaaaagttt
     1441 gtggcgtagc catcactaac atcaacaatt tcatcctttt ttccgacctt tttaacgtct
     1501 tttaataaaa ttactttcat tttatcctcc cttcttgccg ccaacgactt tggcttgact
     1561 tagattttga ttaattacat cttctaaaat ttcttggaca tcatctaatg tcttatccgt
     1621 aaaaacagct gcagcgcttg cttggtgacc accgcctcca actttctcaa gtaagaattg
     1681 aacattaatt tcaccgttac ttctaccgct gactttaacg accttaggcg ccgtgtaacc
     1741 aataacaaac gaagcttgta tacctttaat gcgcatgctt tggtttgcaa ctttcgataa
     1801 agtttctggg gtaacaatgt ctgtttcatc tgctcttaag aagacaatac caacactaag
     1861 tgttgtcatg ctattcataa ttttggtaat aagtgcatat tcttcaacat tatcctttaa
     1921 gaaatcgtcc gttgtcataa catcagcgcc ccaatcttct aacaccatac ttgctaagaa
     1981 agtacgtgat cctgttgttt ttgttctaaa gtagtttgtg tctaaataaa ttccagcaag
     2041 cataaaggtc gcatattttg gtggtaatac gaagttagga gtattaccgt aatgtagtag
     2101 ttcagtcaca atttcacaag ttgaacttgc gcttgtttca atatagtcat aaagtctttg
     2161 atcaataaat tcgtctgtgc gacgatgatg gtcaatcaca attaccttat ccacgcgttc
     2221 aaggagtttt ggatgaagcg ttaattgtgg acatgaaaca tcagcaataa ttaaaagtgt
     2281 gccggaatta actagactcg cggttccttt ttgatcgtca atttcttctg gtgaaataaa
     2341 catatcattc atttcttgtt tcgtaaacat tttgtttaca gccattcttg tttttaattc
     2401 agttaaattg tgttcataaa ctatgcgtgc aggttgaaat tgaacctttg tgcctttttg
     2461 tgttttcttt acatagtcgg ccatagcttt taaaccaagc gacgcaccca aagcgtcaaa
     2521 gtcagtatcg gtgtgtgtca tgattaaaac attttttgct tctcttatat gccggaaaat
     2581 tgtatccgca tataacctaa tcgctgtttt attacgtttt tcagcttgtg aagttgcgcc
     2641 accgataaat tcactttctt caccgtaacg ggcaataaca acttggtcac caccacgcgc
     2701 cattgctgta tcaatcgcat taaggacgag ttcatttagt tttaaaacat cggggaaatt
     2761 atacgcaaac gccattgata acgaaatttg ataatcttga ttcgatgttt tatctttgat
     2821 tttttgtaaa attgaaaaat tatcagcgta taacttatca aagttctctt tgtttaaaat
     2881 aagtagataa gcatcttcac gatattgttt aagcagcacc ccataatttg aaaagtattc
     2941 gagaattaaa ctgttaactg tcccaaaaat tggattagag tgttcacctt taacaacaat
     3001 atccacaaag ttatcaataa ccactacgcc taaaacaggt gctgttgctt gattactatc
     3061 aaagagatat tcaaattcag taatatcttt aaacaagaaa ataccagcct tgcttaaata
     3121 tttaacttcg tagttccgtg attcgatttc aactttaatt gtgtcttcaa cattatctgc
     3181 aagcacttca cttagttcaa caagccaaat taattcaggc ttccaagcaa gaatgttttt
     3241 atcaataatt aaaagacgac gttctttaaa taattcactg gcccatgtga cattaaaatc
     3301 attatcgacg acaacaagac ctagcattcc aaagttgtaa gcctctttaa catcgccacc
     3361 taataagttt acaagttcaa atgtattctt tttccgtaat ttatttattc gtgcagtaat
     3421 gaaaataaaa acgaacaaat taaagaaaac gacacctatc gcgacagaaa aaataactgg
     3481 cggtacaaag atttcttgaa aattaaataa gtttaaataa tagaagagac taattaaacc
     3541 tagtaaaagc ccttcaacta taacaaggat aatcgttcgt ttgcgtaatt tagtaatagc
     3601 tttgatcata gcaatacctc gtttttcttt agagtaatta tacaataaaa aaggtatctt
     3661 taataccttt catgtcccat aatatatatt ttgttgaaaa ttattacttt aatgttgata
     3721 actagtgtaa ttaagacccg gcagaactca tgtattcatt aatgagcgcc ataatttcac
     3781 caagatgttt aaacacaaat gttaatggtt tcatcgctaa tacaatcgcc atgatattta
     3841 aaccaattcc aattaagaca aaaagccaat ttaggccacg taacttaagc attcctaaaa
     3901 gaccatagat tagtcccgtg ataacaataa gtagtgtaaa gactgcaaaa taagcattaa
     3961 atgtcagcga aagaatagaa aagattaagg caatcacacc tgcaatgatt cctaaaaacc
     4021 gataattaaa tactttttga tagaagcgca gttgtttacg taattcgcgt tcaggtaaaa
     4081 gataatatcg acctctgtaa tgatagcggt ctgttaaagt tttaccgcca cgcgtttcgc
     4141 ttccgtcctt ccgctctttg gggtcttttg gttctttttt ctttttcttg cgtttctttt
     4201 ttgctttgcc ttcatcatct tctaatgatt cagcataaac ttcttcatca gcagcgtact
     4261 ttaaatcatc ttcttcgtat gaagagtcat catataaagc aacatcattc tttttctttc
     4321 tggccataat ttaattccta tatatacaat ttagcataaa tttgattatg aataaacaat
     4381 ttgtttatta gcgattttat tttaatgatt atacaaccgc tttttctttg cggggtaaga
     4441 gcggattaat cgctccaatt attgcaaaga cgggagaaag taatatacca ctgatgaata
     4501 ctagcgtact aagattattg caacccttag cctcaaggcg aaggttaacc gctaaaatac
     4561 cttggaacac aaaaataatt gtgacagaaa agaaaacagt aaagatcgcg ttgatactag
     4621 aaattcctag cagatttaat tcctttgtta aaaggaaaga tagtactaaa actactacac
     4681 aaccaactaa ataagttatt gccgcgacac gcgaaattgt aaactctaaa ccgtgtaatg
     4741 ggccgtgttc cattttttct ctgcagataa attttcgtgt taagtgaatg agaatatgcg
     4801 ttaataaaac acttataaaa ctaaatacaa ttgtgtaagc aataacacca tttttaataa
     4861 taacaccgac attttgcgcg taatttgatg tttcatctat cttaaatagt ttaataaata
     4921 attctagata ctgaccaaat tggatatcat aagcaaagtg cggatctttt tgaac
//
LOCUS       DAOSEX010000030         4964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_15102_length_4964_cov_2.788144, whole genome shotgun sequence.
ACCESSION   DAOSEX010000030 DAOSEX010000000
VERSION     DAOSEX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15102_length_4964_cov_2.788144"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..315
                     /locus_tag="PK612_01075"
                     /pseudo
     CDS             1..315
                     /locus_tag="PK612_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223342.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
     gene            524..599
                     /locus_tag="PK612_01080"
     tRNA            524..599
                     /locus_tag="PK612_01080"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:557..559,aa:Asp,seq:gtc)
     gene            604..679
                     /locus_tag="PK612_01085"
     tRNA            604..679
                     /locus_tag="PK612_01085"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:637..639,aa:Phe,seq:gaa)
     gene            796..1593
                     /locus_tag="PK612_01090"
     CDS             796..1593
                     /locus_tag="PK612_01090"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOH94743.1"
                     /translation="MTAFMKLSIFNLICLIVIGILLFLCKKFIKERRTHDLILKTTSI
                     LVILIHYSAVYVEFFQNQGVAYASASYILPVYPCHIMMWLLVIVAFTRHKKSRFFEGL
                     AEFVFVVGTLAALIGALFNMNFLNSSNPTFTNYFLFKGVFGHMVMFFGTTYLFVFEYV
                     KPRVEKTMISVIVGLLFFVADGIIINTLYAKFDIPPVNAMFLLEPPFEDYPFINFFTI
                     GSIGLILCFIGLLIYEHITLPKAEQWITKISTKSNKQDEDLTTDKTN"
     gene            1647..2447
                     /locus_tag="PK612_01095"
     CDS             1647..2447
                     /locus_tag="PK612_01095"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOH94744.1"
                     /translation="MFNLMHELFKDKDTGVFTFSAFSLCHIIYLLLIIGGIILVIFLY
                     KNKTQEAKTKLITTTVTIAFALYIADFFLMPFATGTIDIGKLPFHLCTLMSVMCFVVR
                     HNKKMEVFKTAFTLLGLIGGLMYITYPAGVAQADGYSYRIVQTVIYHGLMIAQGVFAL
                     VFDDLDLSWKNFKYDVIAVLAVAVWAYIGNTLYSGSNFWLSYEHDFNWFFVKHDGLYL
                     IDDEKDAFIAPLMSVSSVLAISALLRLIAIKLKSLFVKEQVLEAEKEA"
     gene            2563..>4964
                     /locus_tag="PK612_01100"
     CDS             2563..>4964
                     /locus_tag="PK612_01100"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HOH94745.1"
                     /translation="MNNKTKSRRLRFRNILLLWLVGLAGQLCWNLENQWFNTFVYAEV
                     GPFPQIVNAMVAVSAIATTFATFFFGTLSDRAGKRKPFISIGYIIWGIFTIAFGLTGL
                     LTKFTMNIWGLATVIVLADTVMSFFGSMGNDAGFNAWTADLISDDNKGAIGTALAVQP
                     VIGTIIGTVLGGILIEAFGYIAFFSMMGGFVSLIGVLSLFLLKDSPKLEPSKRGSFWR
                     QFAEAFNFKYFFSQKELVLVNVVAAIYFIGFNVYFVHIGNLFISNYGFNAGDAGIIQG
                     IGLIIAILFTIPGSRLINRGKSPRVALISILMTIVGLIYLGLIGGYHDPANLFGAKNL
                     PLLLGVIVVGAGYILIMQTLGVWSKVLYPVGSKGQFEGLRIIFFVLIPMVLGSSISQP
                     IIRHFGIETVVEVLEDGTKVMGYIPTEALFYFASIIMVLALIPLNFLRKEYYKRMRNT
                     KISEAVSTAKEELGVDELYYTTPTPVFDKQKRHINFGYATDMSFVFDKKLVKKPLRLK
                     EWDFYQIQHGRYVVQLTLGHIAYAQNIAINLIDLEGEETYSASVVKLFKGAKSMTMPT
                     DPSVDNEIHYTDTKKKVDLHYVTKGRIRHLYGSMVNEKGVTFKVDVKLERLENHDAMV
                     INTPFEDSLYHFYLNYKIGNLRASGTFSMDDLAFTFDPKVDFGLLDWGRGVWPFHEVW
                     YWGQVNDYLADGRMINLNLGYGFGNLEAASENMMIIDGKAYKLGRLYIENDVTLDYMA
                     DWIIKSDDGKIDLVMKTIYDRYSDNDFKVIFMKCHQVYGRYYGTITLDDGTVVELTGQ
                     QG"
BASE COUNT         1453 a          817 c          969 g         1725 t
ORIGIN      
        1 gcatttgcta agaactttca gatttgggac atcagcatcg gagtattata tgttgctgcg
       61 gtttcatcgg tagctgtaat cggaatattg atggctggct ggtcgagtaa caacaaatac
      121 tcattactag gagctatgcg aagtgcagct cagattgtga gttatgaatt atcggcaggg
      181 ctttcgatac ttgcaattgt aatattaagt ggaagtttaa aaattagtga tatcatcaac
      241 tcgcaagcag atggatggtg gatcattaaa ggacatatac ctgtaatcat cgcatttatt
      301 gtttttatta ttgccttaat tagtttctaa aggtttgttc atataccatc tttattatac
      361 aagattagcg ccatcttaag aggcaataca taatgttaac tatcatttat tactatgtaa
      421 taagcgaaat tctaaagtat ttttgcaaaa gtaattcata aaatcaaaac atcgtattga
      481 aatacttgca aaaaagttgg aactttaata tagtatatag gctggaccag tagtgtagtg
      541 gttaacatgt ctgcctgtca cgcagaagat cgcgggttcg agtcccgtct ggtccgccag
      601 taaggcccga tagctcagat ggtagagcag tggactgaaa atccacgtgt cgccggttca
      661 attccggctt gggccaccat tattgcgcga aaacggactt gaaacagtcc gtttttttct
      721 tttattacaa attagtaata tttattaaat ttcatttgat ataatgaaat ggttgtaata
      781 agagatgaat agtttatgac agctttcatg aaattatcaa tatttaatct catatgtcta
      841 atagtgattg gaatattgct ttttttatgc aagaaattca tcaaagaacg tcgaactcat
      901 gatcttattt taaaaactac ctcaatctta gttattctta ttcactatag tgcagtttat
      961 gttgaattct ttcaaaatca aggtgtagca tatgcatccg cttcttatat tttaccggta
     1021 tatccatgtc acattatgat gtggttgctc gtaatcgttg cttttacgcg tcataaaaag
     1081 tcccgcttct ttgaaggact agcagaattt gtattcgttg ttggcactct tgcggcttta
     1141 attggagcac tctttaatat gaattttttg aatagtagta atccaacatt caccaactat
     1201 tttcttttta aaggtgtatt tggtcatatg gtgatgtttt ttggtacaac atacttattt
     1261 gtttttgagt atgtaaaacc acgcgttgaa aaaacaatga ttagtgttat agttggactt
     1321 ctcttttttg tcgctgatgg tattattatt aatacacttt atgcaaaatt tgatatccca
     1381 cctgttaatg cgatgtttct tttagaacca ccgtttgaag attatccgtt tattaatttc
     1441 ttcactatcg gttctattgg gctaattttg tgctttattg gactgctaat ttacgagcac
     1501 atcaccctgc ccaaagctga acaatggata actaaaatat cgactaaatc aaataaacaa
     1561 gatgaggatt taactactga taaaactaat taagtcattt ttagaaagga ataattattc
     1621 actactaata attaaaaaga tattatatgt ttaatctaat gcatgaatta tttaaggata
     1681 aagatacggg tgtctttact ttctcggctt ttagtttatg ccatattatt tatttattat
     1741 taattattgg tggaatcatt ttagtaattt tcttatacaa aaataaaaca caagaagcaa
     1801 agacaaaact tattactacc actgtgacaa ttgccttcgc actttatatt gctgactttt
     1861 tcctcatgcc ttttgctacc ggcacaattg atatcggtaa gttaccgttt cacctatgta
     1921 ccttaatgag tgttatgtgt tttgtagtgc ggcacaataa aaaaatggaa gtatttaaaa
     1981 cagcctttac ccttcttggg ctaatcggcg gattaatgta cattacttat cccgcaggtg
     2041 tggcacaagc tgatggctat agttaccgta ttgtgcaaac agtaatttac cacggtctta
     2101 tgatcgcaca aggggttttt gcgctagtat ttgatgattt agatttgagt tggaaaaact
     2161 ttaaatatga tgttattgcc gtcttagcgg tagcagtatg ggcctatatt ggtaatacgc
     2221 tctatagcgg aagtaacttc tggctttctt atgaacatga ctttaattgg ttttttgtaa
     2281 agcatgatgg gttatattta attgatgacg aaaaagatgc atttattgcc ccattaatgt
     2341 cggtgtcatc tgtccttgct attagtgcct tacttcgttt aattgctatt aagcttaaaa
     2401 gcctctttgt taaagagcaa gttctcgaag cagaaaaaga agcctaaaat tcacttatat
     2461 ttcgtatttt tccgtttatt agagagcttt tttatttatt tattaaagca gtttaacatc
     2521 tagcaaaaat aagataaaat acatttgata ggagaaaaaa ttatgaataa taaaacgaaa
     2581 agtcgtcgtt tgcgttttcg caacatttta ctattgtggc ttgttggtct tgctggacaa
     2641 ttatgttgga acttagaaaa ccaatggttt aatacatttg tttacgctga agttggtccg
     2701 tttccgcaaa ttgtcaatgc aatggtcgct gtttcggcga ttgctacaac ttttgccaca
     2761 ttcttctttg ggacacttag tgaccgggct ggtaaaagga aaccattcat cagcattggc
     2821 tatatcattt ggggtatctt cacgattgct tttggtttaa ctggtctact tacaaaattt
     2881 acgatgaaca tttggggact agcgactgtt attgtccttg ccgatacagt tatgtcattt
     2941 ttcggatcaa tgggaaatga tgccgggttt aatgcctgga ccgctgactt aattagtgat
     3001 gataataaag gggcaatcgg taccgcactt gcggttcaac ccgtcatcgg cacgattatt
     3061 ggtacagtac taggcggaat tttaattgaa gcttttggct atattgcctt cttctcaatg
     3121 atgggcggct ttgtgagctt aattggtgtc ctttcgcttt tcttacttaa agattcacca
     3181 aaattagagc caagtaaacg cggtagtttt tggcgtcaat tcgccgaagc ttttaacttt
     3241 aaatatttct ttagtcaaaa agaacttgta ctcgttaatg tcgtcgctgc tatttacttt
     3301 attggtttta atgtttattt tgtccatatt ggtaatttat ttatttcaaa ctatgggttt
     3361 aacgctgggg atgctggtat tattcaagga atcggcttaa ttattgcgat actctttacc
     3421 atacctggca gtcgcttaat taatcgcggc aagagtccgc gcgttgcgtt aatttcgatt
     3481 ttaatgacga ttgttggcct catttattta ggattaattg gtggttatca tgatccagcg
     3541 aatttatttg gcgccaaaaa tctcccgctt ctactcggtg ttattgttgt tggtgcaggt
     3601 tatattttaa ttatgcaaac gctcggtgtc tggagcaaag tgctctaccc tgttggtagt
     3661 aaaggacaat ttgaaggatt acgcattatc ttctttgtgt taataccgat ggtgttaggt
     3721 tcaagtatta gtcaaccaat cattcgccat ttcgggattg aaacagtcgt tgaagtttta
     3781 gaagacggca caaaagtgat gggctatatt cccactgaag cacttttcta tttcgcttct
     3841 attattatgg tactagcgct tattccgctt aacttcttac gcaaagaata ttataaacgg
     3901 atgcgtaata caaaaattag tgaagcagtc agtaccgcta aagaggaatt aggtgttgat
     3961 gaactatatt acacaactcc taccccagta tttgataaac aaaaacggca tattaatttc
     4021 ggttacgcga ctgatatgtc atttgtattt gataaaaaac tagtgaaaaa gccactccgc
     4081 cttaaagagt gggacttcta tcaaattcaa catgggcgtt atgttgtcca gctcacactt
     4141 ggccatattg catatgcaca aaatatcgcg attaatttaa ttgatttaga aggtgaagaa
     4201 acttattccg caagtgttgt taaactcttc aagggtgcta agagcatgac tatgccaact
     4261 gacccatcag tggataatga aattcattac accgacacga agaaaaaagt tgaccttcat
     4321 tatgttacca aagggcgaat tcgtcactta tacggctcaa tggttaatga aaaaggtgtg
     4381 acttttaaag ttgatgttaa acttgaacgc ttagagaatc acgatgcaat ggttattaat
     4441 acaccatttg aagattcttt gtatcatttc tatctcaatt ataaaattgg taatttacgc
     4501 gcaagtggaa cgtttagcat ggatgactta gcgtttactt ttgacccgaa agttgacttt
     4561 ggcttacttg actggggtcg tggggtgtgg ccattccatg aagtatggta ctggggtcaa
     4621 gtaaatgact acctcgcaga tggacgaatg attaatctaa acttaggcta cggttttggt
     4681 aatttagagg ccgctagtga aaatatgatg attattgatg gtaaagctta taagcttggt
     4741 cgcctctata ttgaaaacga tgtaacgctc gattacatgg ctgactggat tattaagtcc
     4801 gatgatggta agatagattt ggtcatgaaa actatttatg accgctatag tgataacgac
     4861 ttcaaagtta tctttatgaa atgccaccaa gtttatggtc gctactacgg tacaattaca
     4921 ttagatgacg gtactgtggt tgaattaact ggccaacaag gctt
//
LOCUS       DAOSEX010000031         4822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_15721_length_4822_cov_3.741347, whole genome shotgun sequence.
ACCESSION   DAOSEX010000031 DAOSEX010000000
VERSION     DAOSEX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15721_length_4822_cov_3.741347"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PK612_01105"
     CDS             <1..939
                     /locus_tag="PK612_01105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOH94746.1"
                     /translation="KEKNYYATDNYGKFVLEPLERGFGQTLGNSFRRLLLSALPGASV
                     FAITIDGARHEFATLPGVVEDVTMITLNLKQLILKIEDKDTTIKRLEVDVVGPAVVTG
                     ADITLPYGVEVISKDLVIANVAEGGHLKMTIFARNGRGYVTSESNKAERQKVGGAIGV
                     IATDSNYSPVTKVNYTVSNTRVGHDENYDRLTLEVWTNGSITPQAAVALSAKILIEHY
                     KPFLDLEEATVDVEVFAEQEAQAPAQMEDKKIEELDISVRSYNCLKRAGIQTVLELAS
                     KTEEEILKVRALGKKSFKEIKDKLKELGLNFRGSVE"
     gene            949..1350
                     /gene="rplQ"
                     /locus_tag="PK612_01110"
     CDS             949..1350
                     /gene="rplQ"
                     /locus_tag="PK612_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002818480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOH94747.1"
                     /translation="MPAQGRKNVRGKAGVRFKAAYTKSKDKAMIRNVTSELIVYGKVT
                     LIAKVGKQVQREVERLITLAKRGDLHARRLAAARLREGIKRADGVKALDVLFNELGPR
                     FKDREGGYTRILKLGPRRGDNAPMVILEFVE"
     gene            1491..2033
                     /locus_tag="PK612_01115"
     CDS             1491..2033
                     /locus_tag="PK612_01115"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOH94748.1"
                     /translation="MRISINTKVSFGVTVFTSVALIIIGAMIVKYNTNLAASLFYLIG
                     IIIALVGFINFLNYISGRKQKVTVSEYLLSILLMIAGILVAIFSESIQAYGLLVIGIL
                     FVMLGIYDIVLYFRSKATITLIVGILRIVIGTAFIVSGFSEVLLANDEFSRKLWLIIG
                     YASIVLGVSFLVLDTFQVTE"
     gene            2036..2698
                     /locus_tag="PK612_01120"
     CDS             2036..2698
                     /locus_tag="PK612_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOH94749.1"
                     /translation="MNYKHIIFDLDGTLLDTIPDLLYNLNTTFSELGLPGKFSTAEMA
                     TFLGSGKDEQIRRALSARRLSHEKYFAKVNALLSVYYAKNTNTYTEIFPGIKETLAEL
                     KARGVKLYVATNKPDHVAKDVVRHYFGDNVFIKVRGDKGDGIIKPNPKFLNAIIKNIN
                     DPLETIMFVGDSYVDYMSAKNAGVKCAIVPHGYDQKVLSIKDKGVIFLKKVTDIISLD
                     IN"
     gene            2745..4079
                     /gene="cysS"
                     /locus_tag="PK612_01125"
     CDS             2745..4079
                     /gene="cysS"
                     /locus_tag="PK612_01125"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOH94750.1"
                     /translation="MKILLYNSLTKQKEEFIPLRKGEVSMYVCGPTVYDYVHVGNMRP
                     VVVFDTLRRFFNHAGYQVIYVSNYTDVDDRIIEKAQKTNKKETEISEFYIEAFADSAT
                     KINALKPTFTPKATQHIESMIAFIKVLIANGDAYENDGEVFFRVSKVETYGELSNVTQ
                     DELQVGARIDANPKKEHPNDFLLWKKTDDAGIKWDSPWGAGRPGWHTECVVMINDIFG
                     KPLIDIHGGGFDLKFPHHENEIAQARAYANTGLANYWMHNGFINLNNQKMAKSTGNFV
                     TAKDFIEEYGATSLRLLLLSTHYRSPVNLSAEIIENTNNEVLRLQRTKQALVRHFALH
                     PLKVTDDSLLLKFEEALADDLNTPNALSELYAVVKDLNILLRNPLKEEEKLAKLANAF
                     EVMLDVLGLDLGVKNLNEEDTKLLKDYENARKKKDFAKSDALREELQKRGIF"
     gene            4079..4810
                     /gene="rlmB"
                     /locus_tag="PK612_01130"
     CDS             4079..4810
                     /gene="rlmB"
                     /locus_tag="PK612_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOH94751.1"
                     /translation="MNNNNQRTQLAYGRNNVLACLDAGIVIKIIFQEGFSDEKIIKLA
                     TKQNLEITYAKKKDLNNMVAGNHQGIVAVVKSFKFNILEELIIAANNVDNPIILVLDE
                     IKDPHNLGAIIRSAEAFGVTGIVMKKDRQVSITPAVMKVATGAQNNILIAQVTNLSQA
                     LEKLKKTGFWVVSTDLKAEQNVSDLKYDFKTALIMGNEHKGVSPLLTKNSDFVVKIPM
                     LGKTNSLNVSVATGVMLALIRQIQL"
BASE COUNT         1614 a          777 c          990 g         1441 t
ORIGIN      
        1 aaggaaaaaa attattacgc aacagataac tatggcaagt ttgtgcttga accactcgaa
       61 agaggctttg gacaaacgct cggtaattca ttccgtcgtt tactcttaag tgcccttcct
      121 ggtgcaagtg tcttcgctat tacgattgac ggcgcacgtc atgaatttgc aacacttcct
      181 ggtgttgtcg aagacgtgac gatgattaca cttaacctta aacaactcat tctcaagatt
      241 gaagataaag atacgacaat taaacgtttg gaagttgatg ttgtagggcc agcagtggtc
      301 actggagcgg atattactct tccttacggt gtcgaagtta ttagtaaaga tttagtgatc
      361 gctaatgtcg ctgaaggtgg tcacttaaaa atgacaatct ttgctcgcaa cggcagaggt
      421 tatgtcacta gcgaaagtaa taaagccgaa cggcaaaaag ttggtggtgc aattggtgtc
      481 atcgccactg attcaaatta ttcaccagtg acaaaagtta attacaccgt gtcaaacact
      541 cgtgtcggac acgatgaaaa ttatgaccgc ttaacgcttg aagtgtggac taatggtagt
      601 attaccccac aagctgcggt cgccctcagt gccaaaatct taattgagca ctataaacct
      661 ttccttgatt tagaagaagc aactgttgac gttgaagtct tcgctgaaca agaagcacaa
      721 gcgccagcac aaatggaaga taagaagatt gaagaactcg acatcagtgt ccgttcttac
      781 aactgcttga agcgcgctgg aattcaaaca gtacttgaac ttgcaagtaa aaccgaagaa
      841 gaaattctta aagttcgtgc tttaggtaaa aaatcattta aggaaattaa agacaaactt
      901 aaagaactcg gccttaactt ccgaggcagt gtagaataga ggtacaatat gccagcacaa
      961 ggtagaaaaa atgtccgggg taaagccgga gtccgcttta aagcggcgta tacaaaaagt
     1021 aaagacaaag cgatgattcg taatgttacg agtgaactta tagtttacgg taaagtaact
     1081 ttaatcgcta aggtcgggaa acaagttcaa cgcgaagttg aacgcttaat cacacttgct
     1141 aaacgtggtg acttacatgc acgcagactt gccgccgcgc ggttacgtga aggaattaaa
     1201 agagcagacg gtgtcaaagc acttgatgta ctctttaatg aacttggacc acgctttaaa
     1261 gatcgtgaag gcggttatac ccgcattctt aagttaggcc cacgtcgtgg tgacaatgcc
     1321 ccgatggtca tcttagaatt cgtcgaataa gacgcgtctt actaaaattc attaagccta
     1381 gccattaagg ttaggctttt ttgtttaaaa aggcatatca cttaaagtga taatactttg
     1441 caaaaagcgt gtgtttaata taatattaaa gaggaataaa ggagaaaagt atgcgcatat
     1501 caataaatac aaaggttagt tttggagtaa cagtcttcac tagtgtcgca ctaattatta
     1561 ttggagcgat gatcgttaaa tataacacta atttagcggc aagtcttttt tacttaattg
     1621 ggattattat tgcccttgtt ggctttatta atttcttgaa ttatatttcc gggcgtaaac
     1681 agaaagttac ggtctcggaa tatttactta gtatcttact aatgattgcc ggaattttag
     1741 tcgctatttt tagtgaaagt attcaagctt acggcttact tgtcattggg attttatttg
     1801 tgatgttagg aatatatgat attgttttat atttccgctc taaagcgaca ataacactga
     1861 ttgttggcat tttacggatt gttattggta cggcctttat tgtttctggg tttagtgaag
     1921 tattgcttgc taatgatgaa ttttcacgaa aactgtggct tattattggt tatgcgtcaa
     1981 ttgtattagg cgtgagtttc cttgtgcttg atacattcca agttactgaa tagctatgaa
     2041 ttataaacat attatttttg atttagatgg aacgctttta gatacgattc ctgatttact
     2101 ctataactta aacactactt ttagtgaatt aggtttaccg ggtaaattta gcaccgcgga
     2161 aatggcgacc tttttaggga gcggtaaaga cgaacaaata agaagagcgc tcagtgcaag
     2221 acggcttagt cacgaaaagt attttgcgaa agttaatgcc cttttgtcgg tgtattacgc
     2281 caaaaacacg aatacttata ccgaaatatt ccccggcata aaagaaacac tagcggaatt
     2341 aaaagcgcgc ggtgttaagt tatatgttgc cactaataag ccagatcacg tcgctaaaga
     2401 tgtagtgaga cattattttg gtgataatgt ttttattaaa gtacgtggcg ataaaggtga
     2461 cgggattatt aaacctaatc caaaattttt aaatgcgatt attaaaaaca ttaatgaccc
     2521 gcttgaaaca attatgttcg tgggcgatag ttatgttgat tatatgtcag cgaaaaatgc
     2581 cggtgtaaaa tgcgctattg taccgcacgg ttacgatcaa aaagtgttaa gcattaaaga
     2641 taaaggtgta atctttttaa agaaagtaac agacattatt tctttagaca taaattagga
     2701 acatagagtg cgttaattac ctgtgttcgt gataaaatat tactatgaaa atacttttat
     2761 acaactcact tacaaaacaa aaagaagaat tcattccgct gcgtaaaggc gaagtatcaa
     2821 tgtatgtatg cggtccaaca gtctacgact atgtgcatgt tggtaatatg cgtcctgttg
     2881 tcgtctttga tacattacgg cgctttttta atcacgcagg ttatcaagtg atttatgttt
     2941 ctaactacac tgatgttgat gatagaatca ttgaaaaagc gcaaaaaact aataaaaaag
     3001 aaactgaaat tagtgaattt tatattgaag cttttgcaga ctcggcaact aaaattaatg
     3061 ccttaaaacc tacatttacc ccaaaagcaa cgcagcacat tgaaagtatg attgctttta
     3121 taaaagtgtt aatcgctaat ggcgatgctt atgaaaatga tggtgaagta tttttccgtg
     3181 tgtcaaaagt tgaaacttat ggtgaattgt caaatgtcac acaagatgaa cttcaagttg
     3241 gtgcgcggat tgacgctaac ccgaaaaaag aacatccgaa tgatttctta ttatggaaga
     3301 aaacggatga tgcaggaatt aaatgggatt caccgtgggg cgcaggtagg ccagggtggc
     3361 acacagaatg cgttgtgatg attaatgata tatttgggaa gcctttaatt gatattcatg
     3421 gtggcggatt tgatcttaag tttccacatc acgaaaacga aatcgcgcaa gcgagggctt
     3481 acgctaatac ggggcttgct aattactgga tgcataacgg cttcattaat ttaaacaatc
     3541 aaaagatggc aaagtccact ggtaactttg taactgctaa agattttatt gaagagtatg
     3601 gcgctacatc attacgctta ttacttttat ctacgcatta tcggagtcca gtaaacctta
     3661 gtgcagagat aattgaaaac actaataatg aagtgctgcg cctacaaaga actaagcaag
     3721 cacttgtgcg tcattttgcg ctacacccac tcaaagttac agatgattca ctactcttaa
     3781 aatttgaaga agctttagcc gacgacttaa atacaccaaa cgctttaagt gaattatatg
     3841 cagttgttaa agacttaaat atcttacttc gaaacccgtt aaaagaagaa gaaaaactcg
     3901 ctaaattagc aaacgcattt gaagtaatgc ttgatgtttt aggacttgat ttaggtgtta
     3961 aaaatctaaa tgaagaagac actaaattgc taaaagacta tgaaaatgca cggaaaaaaa
     4021 aggattttgc gaaaagtgac gctttacgcg aagagttgca aaaacggggt attttttaat
     4081 gaataataat aaccaaagaa cacaattagc ctacggacga aataacgttt tagcgtgttt
     4141 agatgcgggg attgtaatta aaattatttt tcaagaaggt tttagtgatg aaaagataat
     4201 taaacttgcc actaagcaaa atcttgaaat tacttatgca aagaaaaaag atttaaataa
     4261 tatggttgcg ggcaatcatc aaggtattgt cgccgttgtt aaatctttta aatttaatat
     4321 tttggaagaa cttattattg ctgcgaataa tgtagataat ccaattattt tagtattaga
     4381 tgaaattaaa gatccacata atcttggggc gataattaga agtgctgaag cttttggtgt
     4441 cacaggaata gtgatgaaaa aagatcggca agtctcaatt actccagcgg taatgaaagt
     4501 tgctactgga gcgcaaaata atattttaat tgcgcaagtg actaatcttt cgcaagcatt
     4561 agagaaatta aaaaaaactg ggttttgggt tgtaagtaca gacttaaaag ctgaacaaaa
     4621 tgttagcgat ttaaaatacg atttcaaaac tgcactaata atgggtaatg agcataaagg
     4681 cgtttcacct ttattaacga aaaattctga ttttgttgtt aagattccga tgcttggaaa
     4741 aactaattct ttaaatgtaa gtgtcgcaac aggcgtaatg cttgcactaa ttcgtcaaat
     4801 tcaattgtag ttattaatag ta
//
LOCUS       DAOSEX010000032         4767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_15988_length_4767_cov_3.106749, whole genome shotgun sequence.
ACCESSION   DAOSEX010000032 DAOSEX010000000
VERSION     DAOSEX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15988_length_4767_cov_3.106749"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PK612_01135"
     CDS             <1..534
                     /locus_tag="PK612_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94752.1"
                     /translation="FSKLKTTIGVASLAFGTLTVLVLFIITKNKALIFTWLNIFIVSL
                     VISIWGLLFDTFNPLNTTFFIRALVALLGILIISFGVFLVIVTEFPAGPPEEIMRLIN
                     KKTNNLFVAKLLAETFYLVLACISMIISVTIIDGNGVEFTQVGIFTIVTLILTSVFVS
                     LFDLAYRKLVVKKVESA"
     regulatory      596..725
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            751..2097
                     /locus_tag="PK612_01140"
     CDS             751..2097
                     /locus_tag="PK612_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOH94753.1"
                     /translation="MDAIFVLILKLAIVLSVGFIGSLVARKLKLPNVSGYLLFGLLLG
                     PSLGLIFKGFPGIITVEDSGNLNFISQIALAFIAFSIGAEFSIKSIKKVGKPILVMTS
                     TEVLGAVLVVFFAMFFLPKPQAIMPDGYNPFSKPNVAFGLILASMSAATAPAATLMVI
                     RQYRAFGPVTRAILPITALDDIFGIVVFGFFISFAQILASTGPLPPVWLMILKPFIEV
                     FGSVALGMIIGLLLSLLANKFDRIRDDLQVLSLIAVFGTIGLTYLINYYLEPHGLSFG
                     QLLSNIMIGSMIANIAKRPEKTFNAINDFATPFYILFFTLAGAGLDLAILGTTPLLLL
                     IAGVYILARGFGKWLGIFVGANVVKAEPTVKKYLGIALLPQGGVSIGLLAIVYTQMAP
                     MYPAISTVIMLSVLVYETMGPVFAKFAISKAGEIGGLDRLEQLSSLDGIELTEEGA"
     gene            2100..2450
                     /locus_tag="PK612_01145"
     CDS             2100..2450
                     /locus_tag="PK612_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94754.1"
                     /translation="MQILFIVLNDLSYLDKILEKFLDLHVRGATIIDSQGMAAAIMQN
                     EGLLSLLSGPFARSLETNQKNSKTIFTVIPEAEKVTEVVKAVQDIISDCKKQVVGFMF
                     TLPVSGIYPLKPKK"
     gene            2503..3042
                     /locus_tag="PK612_01150"
     CDS             2503..3042
                     /locus_tag="PK612_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94755.1"
                     /translation="MIMRKRKVNEFTEYLNHLNADDVQKVKAFMVNFKQHMNLPLSEE
                     KKLRKDLKGALMYYNAIGVSVTEALRRLDNENLGGFYARPPLLYYALDDAAKIYPLSM
                     KHGFQAVFRLSANLYDEVVPQLLQIALTFTIKRFPRFATTLKKGFFWHYLDANKQRYG
                     IEKEEHLPCRPIKVATTGS"
     gene            3295..4026
                     /locus_tag="PK612_01155"
     CDS             3295..4026
                     /locus_tag="PK612_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94756.1"
                     /translation="MSGKIESIRPTQILHFRLDAKELAKTAKSYDVTITTYVLALILL
                     ASRFATDAKEGDIAIQVPVDLRKYYESTLANFSLYGGIRIPITSITTMEQLLPLVKEQ
                     LVTATSHEKMSEMMYSAHRMNKDVRGFPLFLKSPIAKLAYGLMGEAIFTTTLSNLGVV
                     SAPDYFSDYIESYDFVLGPPEVNRAALTLITYNNIATLTITKSTKDPSFEEEIHRLLT
                     KDGLDITIEGSAVYERKSRVSRKKY"
     gene            3989..4564
                     /locus_tag="PK612_01160"
     CDS             3989..4564
                     /locus_tag="PK612_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94757.1"
                     /translation="MNVKVVYPAKNIRHDIRRAAIYWAKPAFILAAIISAVVNILVKG
                     SAWSVIVIWSLWMIWSFVFTPTLIEHNRTSIAVKSSIHVTILIVIIYMIYPSWPGIEV
                     ASLVVVGGLIITAILFFSNVSRQKQNVFPLLIFIIIAIIFSVVAFVLRGQEPLGWAMI
                     VSISVALAIFLSTAIILRINFFRELKKRFML"
     gene            4582..4655
                     /locus_tag="PK612_01165"
     tRNA            4582..4655
                     /locus_tag="PK612_01165"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4614..4616,aa:Gly,seq:ccc)
BASE COUNT         1404 a          900 c          892 g         1571 t
ORIGIN      
        1 tttagtaaac ttaaaacaac aatcggtgtt gcttcattag cgtttgggac actaacagtg
       61 cttgtcttat ttattattac taaaaataaa gccttaattt ttacatggct aaatatattt
      121 attgtttcac tcgtaatttc catttggggt ttactttttg atacatttaa cccgcttaat
      181 actacatttt tcatccgtgc actagttgct ttacttggga ttttaattat ctcttttggt
      241 gttttccttg tgattgtcac agaattccct gctggtccac ccgaagaaat catgcgtcta
      301 attaataaaa aaacaaataa tctttttgtc gctaaattat tagccgaaac tttttattta
      361 gttctagcat gtataagtat gattatctca gtaacgatta ttgatggtaa tggtgtggag
      421 tttacccaag ttggtatatt tacaattgtg acactgattt taacttctgt gttcgtatca
      481 ttatttgatt tagcgtatcg aaaactagtc gtaaagaaag tagaatccgc ttaacgcgga
      541 tttttttata gtcacgcacc aaaaaaaatt gacttaccat actatatcta gtaagatata
      601 ggggactccg aatccgaaga gtgggggacc caacagtcgt ttggggtgaa tcaattataa
      661 ttgtaaggat attcttaatg tcctgaaccc gacagctaac ctcataggag taattaagaa
      721 cattaattat ttttatgaga ggagaaaatt atggatgcaa tttttgtttt aatcctaaaa
      781 cttgccatcg tactatccgt gggatttatc ggatcactcg tggcccgaaa attaaaatta
      841 ccgaatgtta gcggttattt acttttcggt ttgttacttg gaccatcact cggtcttatt
      901 ttcaaaggct ttccgggcat tatcactgtc gaagatagtg gcaatctcaa ctttataagc
      961 caaatcgccc ttgcgtttat cgctttttca attggtgcgg aatttagtat caaatcaatt
     1021 aaaaaggtcg gtaaaccaat tttagttatg acttcaactg aagtattagg tgcggtatta
     1081 gtcgtattct ttgcaatgtt ctttttacca aaaccacaag caattatgcc tgatggttat
     1141 aacccattta gcaaacctaa tgtggccttt ggtttaattt tagcgtcaat gagtgcggcg
     1201 actgccccag ccgctaccct tatggttatt cggcaatatc gggcttttgg tcctgtgaca
     1261 cgcgctattt taccaattac cgcgcttgat gatatctttg gtattgttgt ctttgggttc
     1321 tttatttctt ttgcgcaaat cttagcgtca actggtcctt tacccccagt gtggctcatg
     1381 attttaaagc catttattga agtgtttggc tcggttgccc ttggaatgat aatcggttta
     1441 ctcttatcgc ttctagcgaa taagtttgac cgcattcgtg atgacttgca agtcttatca
     1501 cttattgctg tctttggtac aatcggatta acatatttga ttaattatta cttagaacca
     1561 cacggtttat catttggtca attattatca aacattatga ttgggtcaat gatcgctaat
     1621 attgctaaac gtcccgaaaa gacatttaat gcgattaacg actttgctac cccattttat
     1681 attcttttct tcacccttgc tggtgcaggc cttgatttag ccattttagg aacaaccccg
     1741 ctcttactat taatcgctgg tgtctatatc ctagcgcgcg gctttggtaa gtggcttggt
     1801 atctttgtag gcgctaatgt ggttaaagct gaaccaacgg tgaaaaagta cttaggcatt
     1861 gcccttttac cacaaggtgg tgtctcaatt ggattgctgg caattgtcta cacacagatg
     1921 gccccgatgt atcctgccat ttctactgtt ataatgttat cagtattagt gtatgaaacg
     1981 atgggtccag tctttgcaaa atttgccatt agtaaagccg gcgaaattgg aggtcttgat
     2041 cgccttgaac aattatcaag cctcgacggt attgaattaa ccgaggaagg agcataatta
     2101 tgcaaatctt atttattgtt ttaaatgact tatcttactt agacaagatc ttagaaaagt
     2161 ttctagatct ccatgtgcgc ggtgcgacaa ttatcgactc acaaggaatg gccgcagcaa
     2221 ttatgcaaaa tgaaggctta ctctcattgc tctccggtcc ttttgcgaga tcacttgaaa
     2281 ctaaccaaaa aaactcaaaa acaatcttta cagttattcc agaagcagaa aaagtgacgg
     2341 aagttgtaaa agccgtccaa gacattattt ctgattgtaa aaaacaagtt gtgggcttta
     2401 tgtttacctt gccagtaagc ggtatctatc cacttaagcc taaaaaataa aatcatctaa
     2461 acatcaataa attcacgatt acataatcgt gttataatta ctatgattat gagaaaacgc
     2521 aaagtaaatg agtttaccga atatttaaat cacttaaatg ctgatgatgt ccaaaaagtg
     2581 aaagctttta tggtgaactt taaacaacat atgaacttac cacttagcga agaaaagaaa
     2641 ttacggaaag accttaaagg ggctctgatg tattacaacg ctattggtgt atcagtaact
     2701 gaagctctgc gccgccttga taatgaaaac cttgggggat tttacgcacg tccccctctt
     2761 ttatattacg cgcttgatga cgccgcaaag atttatcctt tatcaatgaa acacggcttt
     2821 caagctgtgt ttcgactatc tgcaaatcta tatgacgaag ttgtaccgca actattacaa
     2881 attgcgctta ccttcacgat taaacgcttc ccgcgctttg ccacaacctt aaagaaaggt
     2941 ttcttctggc actatttaga cgctaataag caacgctacg gtattgaaaa ggaagaacat
     3001 ttaccatgtc gtccgattaa agtcgctaca acaggttcat aatcattccg cgttttatat
     3061 tataaaaatc ggattagtgt tgaatttttc cattcgttaa cagacggaaa aggcggaatc
     3121 gtctttttac aaacactact tgatacttat ttacgtcttt ttggtgttga aggacacaag
     3181 catccaagtg tactcgatat taatgccgca ccacttcctg aagaagatgc aaacgaattt
     3241 aaaagaacaa ctgtcacaaa gcgcagcgga tttaaagaaa aacgggcctt acaaatgagt
     3301 ggtaaaattg aaagcattcg cccgacacaa atattgcatt ttagacttga tgcgaaagaa
     3361 ctagctaaaa ctgccaagtc atatgatgtc acgattacaa cttatgtctt agcgctaatc
     3421 cttttagcaa gtcgctttgc aacagacgct aaggaaggtg atattgcaat tcaagtacct
     3481 gttgatttac gcaaatatta tgagtctact ctcgctaatt tttcgcttta tggtgggata
     3541 agaattccaa ttacaagtat tactacaatg gaacaacttt taccacttgt caaggaacaa
     3601 ttagtaacgg ccacaagtca tgaaaaaatg agtgagatga tgtattccgc acaccggatg
     3661 aataaagatg tccgcggttt cccgcttttc ttaaagtcac cgattgctaa acttgcctat
     3721 ggactgatgg gtgaagcaat ctttacgaca acattaagta atttaggggt tgtaagtgcc
     3781 ccagattatt ttagtgacta tattgaaagt tatgactttg tgcttggtcc acctgaagtt
     3841 aatcgcgcag cattaacatt aatcacttat aataatatcg ctactttaac aattacgaaa
     3901 agtacgaaag atccatcctt cgaggaagaa atacatcgtc tgcttacaaa agatggttta
     3961 gacataacga tagaagggag tgctgtatat gaacgtaaaa gtcgtgtatc ccgcaaaaaa
     4021 tattagacat gacattcgtc gtgccgctat ttactgggca aagcctgcct tcattttagc
     4081 ggcaattatc agcgctgttg tcaatatctt agtgaaaggc tcggcctgga gcgtgattgt
     4141 catatggagc ttatggatga tatggtcatt tgtgtttact ccaacactta ttgaacacaa
     4201 tcgcacaagt atcgccgtga agtcttcgat tcatgtcact attcttatcg tcataattta
     4261 tatgatttat ccaagttggc cgggaatcga agttgcttca ctcgtggtcg ttggtggttt
     4321 aattattacc gctatattat tttttagtaa tgtctcacgt caaaagcaaa atgtctttcc
     4381 gcttttaatc tttattatta tcgctattat ttttagtgtc gtggcctttg tattacgcgg
     4441 tcaagaacca cttggctggg cgatgattgt ttctatcagt gtcgcacttg ctatattttt
     4501 aagtacggca attattttaa gaattaactt tttccgtgaa ctaaaaaagc gatttatgtt
     4561 atagtttatt gtagaaaaaa cgcagctgta gtttagtggt agaactttag cttcccaagc
     4621 taaccgtgcg ggttcgattc ccgtcagctg ctccatctag attgaaattg cttcctaaag
     4681 ttgcatcaaa aactactgca acaaaaaggg agctttttta tttaatcatt ttttccagca
     4741 aaaacgcatt gttattactt cacgcaa
//
LOCUS       DAOSEX010000033         4750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_16071_length_4750_cov_3.161448, whole genome shotgun sequence.
ACCESSION   DAOSEX010000033 DAOSEX010000000
VERSION     DAOSEX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16071_length_4750_cov_3.161448"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..688
                     /locus_tag="PK612_01170"
     CDS             182..688
                     /locus_tag="PK612_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOH94758.1"
                     /translation="MGMLHGELASEEKDAALLNFKSGVTPILVSTTVVEVGIDVKSAG
                     LMIIYEPHSFGLSSLHQLRGRIGRDGKRATLCLVSEEEDNYKLEEFAREDNGFKIAEL
                     DLALRGPGELTGEKQSGMPSFMYLNVIKDLKLITIIKEDALEVINNPTNPHYFPLLKV
                     INDALEKD"
     gene            940..1926
                     /gene="plsX"
                     /locus_tag="PK612_01175"
     CDS             940..1926
                     /gene="plsX"
                     /locus_tag="PK612_01175"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOH94759.1"
                     /translation="MKLVIEMMGGDYGFESTIPALIQFKKDYPEVELFAVGRKEDLAP
                     VADYATIVDARDVVKIDATPLEVLRAKESSLLKAINTVIEEKADALISAGGTGPYLSA
                     ATLKLKLIEGVERAAILAPFPTKIKGKQVAILDIGASNENTASHLYQFALMGQQYARA
                     VFGVEAPKTYLLSNGTEDTKGSPLVLEAFKLYKEKELPYFMGQMEARDALNGEADVVI
                     ADGFTGNVLLKSIEGTAKVLMDSVKGAFKRNLFSKLGYLFARKGFKNMAETFDYKNTG
                     GAILIGVNNVVVKAHGNSDPRGFYSAILVGYKMAKADIVNKVREGLKNVETK"
     gene            1910..2590
                     /gene="rnc"
                     /locus_tag="PK612_01180"
     CDS             1910..2590
                     /gene="rnc"
                     /locus_tag="PK612_01180"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOH94760.1"
                     /translation="MSKLNSLFTKLGITPSNEGLFEEALTHSSYKTKDKDAHDYERLE
                     FLGDAVLNMLIASAIYKSHPDKKQGELTNMRKNFVRTQTLATLGRKLELERYIRTGKS
                     ITPQDLYSNDKYFEDVFEALIGAMYLDQGLKVTEDFILRMLGSAIEQYDFNQIFDYIS
                     TLQEFMQADKRGLPQYITEPSGYDNKEEAFISTVTYDGIILGCGYGANSKEAEQNAAK
                     NALEKVAR"
     gene            2592..3572
                     /gene="ftsY"
                     /locus_tag="PK612_01185"
     CDS             2592..3572
                     /gene="ftsY"
                     /locus_tag="PK612_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOH94761.1"
                     /translation="MGLISFLKKKFGGKKNETTDKYVVGLEKSKANFSSKLKRLAKRY
                     EKVTEEYYEHLLEILIGADVGVTLTYEIIEATKEEALLNNLTDPEAINDLLVDKMFIG
                     YLKQGEDIVNEIQFADNGPTVLLVVGVNGSGKTTSIAKLAHRYKKEGKKVLLVAGDTF
                     RAGAVAQLEVWAKRLDVPIVTGKPNADPASVAYEGVAEGKKINADLIIIDTAGRLQTK
                     VNLMAELSKIRRVIGDQIENAPHEVFLIIDATTGQNGVLQAKAFAEATGLTGVVITKM
                     DGTSKGGIILAIRDEIGVPVRFIGLGEKMEDLDEFDLEKYLYGLCLGADE"
     gene            3572..3850
                     /locus_tag="PK612_01190"
     CDS             3572..3850
                     /locus_tag="PK612_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94762.1"
                     /translation="MVVISLLSLVVSFLVFMGVRFAVYSLNPSNYLFADILIIIIGSF
                     IVSLIFAIRQFRYMGRDKWRSQFFHSFVLRTLLINFAIIFIISLLWSW"
     gene            3856..4182
                     /locus_tag="PK612_01195"
     CDS             3856..4182
                     /locus_tag="PK612_01195"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94763.1"
                     /translation="MDELKEYMYINELLLIYGKTLTKRQLKIMESYYKYNLGVQEIAE
                     ELNITKAAVSDAIKVSTKHLENLESIVGHYAYRQQVEALHEEIKNTSTLQEVKELLSK
                     EEDDGI"
     gene            4169..>4750
                     /locus_tag="PK612_01200"
     CDS             4169..>4750
                     /locus_tag="PK612_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOH94764.1"
                     /translation="MMAFESLTERFSKILKKVRGQSRLTEKNMDEMLQEVRIALFEAD
                     VNFKVVKEFVNNIKEKALGTKVYDTLNPGQMVVKIVNDELVELLGSTDTELIFEKNKP
                     TIIMLVGLQGTGKTTTAGKLALHFKKKMNKRVLLAAADVYRPAAIDQLDTLAKQVGVD
                     IVNLGDKVSPVEIAKTAKKKAYDEAVASLERMKE"
BASE COUNT         1648 a          709 c         1023 g         1370 t
ORIGIN      
        1 atactgggcg acaagtgaat accattatta cgacaagtgc gtctaaaaat gtgataccgc
       61 ttattgacaa aacactctcg gaaaatagac aagtttatat agtcgcacca agattagttt
      121 cggtgataaa acaaatgttt tagcgcttta cgagcgtttt tcagcgcgct acccacattt
      181 agtgggtatg cttcacggcg agcttgcaag cgaagaaaaa gacgcggcgc tcttaaactt
      241 taaaagtggt gtaacaccaa tcttagtgtc aacgacagtc gttgaagtcg ggattgatgt
      301 gaagagtgcg ggacttatga taatttacga accacatagt tttggactta gttcattgca
      361 tcaacttcga ggcagaattg gccgcgacgg gaaaagggca acactttgtt tagtgagtga
      421 ggaagaagat aattataaac ttgaagagtt tgcaagagaa gataacggct tcaaaatagc
      481 agaacttgat ttagcactcc gcgggcctgg tgaattaacg ggagagaagc aaagcgggat
      541 gcctagcttt atgtatctta atgttattaa ggatttaaaa ctaattacta tcattaaaga
      601 agatgcgttg gaagttatta ataacccgac aaatccgcat tattttccgc ttttaaaagt
      661 aattaatgat gctttagaaa aagactaata taaatatctg tctaatattt ttgcaggaac
      721 atacactttt aatcaatagg aattatagtt actttttcat tgttttaagt cacaaaatac
      781 gaaattgtcg tctcattata aaggttattt ttaatttgac tttcaagatt taaaaaaatt
      841 gaaagagttg aaagtgcaga gctttaaacg gcttagttta tataaaaagg tgattatggt
      901 aaaataactt tggttatttt taataaggag ataacgacta tgaaacttgt aattgaaatg
      961 atgggtggcg attacggatt tgaaagcaca atcccagcgc ttatccagtt taaaaaagat
     1021 tatccagaag ttgaactttt tgctgtgggt cgcaaagaag atttagcgcc agttgcggat
     1081 tacgcaacga ttgttgatgc gcgtgatgtt gttaaaattg atgcgacacc gcttgaagtg
     1141 ttacgcgcaa aagaaagttc gcttttaaaa gcaattaata cggtaataga agaaaaggct
     1201 gatgcgctta ttagtgcagg cggaacaggc ccatatttaa gtgcggcaac gcttaaactt
     1261 aaattaattg aaggtgttga acgggccgcc attttagcgc ccttcccaac gaaaataaaa
     1321 ggcaaacaag tcgcaattct tgatattggg gcaagtaacg aaaatactgc gagtcactta
     1381 tatcaatttg ccctaatggg acaacaatac gctagagcgg tatttggagt agaagcacca
     1441 aaaacttatt tattaagtaa cggaactgaa gacaccaaag gttcaccttt agtgcttgaa
     1501 gcttttaaac tttataaaga aaaagaatta ccttacttta tgggtcaaat ggaagcacga
     1561 gacgcgttaa atggcgaggc tgatgttgtg atagcagacg gctttactgg aaatgtgtta
     1621 cttaaatcaa ttgaaggaac cgcaaaagtc ttaatggaca gtgtaaaagg cgcttttaaa
     1681 cgaaacttat tttctaaatt aggatatctc tttgcaagaa aaggtttcaa aaacatggca
     1741 gaaacctttg attataaaaa tacaggtgga gcgattttaa ttggtgttaa taatgttgtc
     1801 gttaaagcac atggtaatag tgatccacgc ggcttttata gcgctattct tgttggttac
     1861 aaaatggcca aagctgatat tgtgaataaa gtacgtgaag ggttaaaaaa tgtcgaaact
     1921 aaatagtctt tttactaaat tagggattac gccaagtaat gagggactct ttgaagaagc
     1981 gttaacgcat tctagttata aaacaaaaga caaagacgcg catgactatg aaaggcttga
     2041 gtttttaggt gacgctgttt taaatatgct gattgctagt gcgatttata aaagtcatcc
     2101 tgataaaaaa caaggtgaat taacgaatat gcggaaaaac tttgtgcgca cacaaacgct
     2161 agctacttta ggacgcaaat tagaattaga acgctatatt agaactggta aatcaattac
     2221 accgcaagac ttatatagta acgataaata ttttgaagac gtttttgagg cattaatcgg
     2281 tgcgatgtat cttgatcaag ggctaaaagt aacggaagat tttattttaa gaatgttagg
     2341 cagtgcgatt gagcaatatg actttaatca aatttttgat tacattagta cgctacaaga
     2401 attcatgcag gctgataaaa ggggattacc acaatacatc actgaacctt cgggttacga
     2461 taataaagaa gaagccttta tttcaacagt gacatatgac gggattattc ttgggtgtgg
     2521 ctacggcgca aatagtaaag aagccgaaca aaatgcagca aagaatgctt tagaaaaggt
     2581 ggcgcggtaa gatgggatta atttcatttt taaaaaagaa gtttggtggg aagaaaaacg
     2641 aaaccaccga taaatatgtt gtcggtctag aaaaaagtaa ggcgaacttt tctagtaaac
     2701 taaaacgctt agcaaaacgc tatgaaaaag taacggaaga atattatgaa cacttacttg
     2761 aaatcttaat tggcgcagat gttggtgtta ctttaactta tgaaattatt gaagcaacaa
     2821 aagaagaagc actgcttaat aatttaactg accctgaagc tattaatgac cttttagtcg
     2881 ataagatgtt tattggttac ttaaagcagg gggaagacat agttaacgaa attcagtttg
     2941 cagacaatgg tccaaccgtg ttacttgtgg ttggtgttaa cggcagcggg aaaacaacat
     3001 caattgctaa actagcgcat cgctataaaa aagaaggaaa aaaagtttta ttagttgcag
     3061 gcgatacctt ccgcgctggt gcggttgcac aacttgaagt atgggcaaag cgccttgatg
     3121 tcccgattgt gacaggaaaa ccaaacgctg atcccgcaag tgttgcttat gagggagtcg
     3181 cagaagggaa gaaaattaac gctgatttaa ttattattga tactgcgggt cgcttacaaa
     3241 ctaaggtgaa cttaatggca gaattaagca agattcgccg ggttattggt gaccaaatag
     3301 aaaatgctcc gcatgaagta tttttgatta ttgatgcgac aactggacaa aacggagtgc
     3361 tgcaagctaa agcatttgcc gaagcaactg ggcttactgg tgttgttatt acaaagatgg
     3421 atggcactag taagggcgga ataattttag cgattagaga tgaaattggt gtgccagtac
     3481 gctttattgg cttaggcgaa aaaatggaag acttagatga atttgattta gaaaaatatt
     3541 tatatggctt atgcctaggg gcggacgaat aatggttgtt atttccttac ttagtttagt
     3601 agtgtctttt cttgtgttta tgggagtgcg ctttgcggta tattcgctta atccttcaaa
     3661 ctatttgttc gcggatattt taattattat cattggtagt tttattgtta gtcttatctt
     3721 tgccattcgc caatttagat atatgggaag agataaatgg cgctcacaat tctttcacag
     3781 ttttgtgctt cgtacactct taattaattt tgcgattatc tttataattt cgttactctg
     3841 gtcttggtga taaaaatgga tgaattaaaa gagtatatgt atattaatga gttattacta
     3901 atttatggta aaacactcac aaaacgacaa ctaaagatta tggaaagcta ttacaaatat
     3961 aatcttggtg tccaagaaat agcagaagaa ttaaacatca ctaaagcagc ggtgagcgat
     4021 gcgattaaag tatccactaa acacttagaa aatttagaaa gtattgtggg gcattacgct
     4081 tatcgccaac aagtggaagc actgcacgaa gaaataaaaa acacttcaac attacaagaa
     4141 gttaaagaac tcttaagtaa ggaggaagat gatggcattt gaatcattaa ccgaacgatt
     4201 tagtaaaata ttaaaaaaag tccgcggtca atcgcgtctt accgaaaaaa atatggacga
     4261 gatgctccaa gaagtgcgga ttgccctttt tgaagcggat gttaatttta aagttgtaaa
     4321 agaattcgtt aataatatta aagaaaaagc actaggcacg aaagtttatg acacacttaa
     4381 ccctggtcaa atggtcgtaa aaattgttaa cgatgaatta gtggaacttt tagggagcac
     4441 tgatactgaa ctaatttttg aaaaaaataa accaacaatt attatgcttg ttggtcttca
     4501 aggaactggt aaaacaacga ccgctggtaa attagcgctg cattttaaaa agaaaatgaa
     4561 taaaagagtg ttacttgctg cagcagatgt ctatcgacca gcggcaattg atcaacttga
     4621 tacactagcg aaacaagtcg gtgttgatat tgttaattta ggtgataaag ttagtcctgt
     4681 tgaaattgcg aaaacagcaa agaaaaaagc atatgacgaa gctgtagcta gcttagaaag
     4741 aatgaaagaa
//
LOCUS       DAOSEX010000034         4593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_16827_length_4593_cov_3.336051, whole genome shotgun sequence.
ACCESSION   DAOSEX010000034 DAOSEX010000000
VERSION     DAOSEX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16827_length_4593_cov_3.336051"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..2486
                     /gene="secA"
                     /locus_tag="PK612_01205"
     CDS             60..2486
                     /gene="secA"
                     /locus_tag="PK612_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOH94765.1"
                     /translation="MSFIEKIFRFEQRRLKKIEKTAKKVIAYEKTMEALTDDELKAKT
                     PEFRERLTNGETLDDLLPEAFAVAREAARRTLGQFPYPVQIMGAIVLHHGDVAEMRTG
                     EGKTLTATMAVYLNALSGKGVHVVTVNEYLAARDADWMGQIYRFLGLTVGVNLREKTA
                     REKREEYLCDITYTTNSEVGFDYLRDNMASSVDGRVLRGLEYAIVDEADSILIDESRT
                     PLIISGGAKVQASSYNVAERFVKTLRRDKHFVVDIKTKTCNLTDEGNKLAERMFGVNN
                     IYDPQYSDLVHRIHNALKANYVMSRDVEYMVDANREIQLIDQFTGRIMPGREYSDGLH
                     QAIQAKENVTIKEETVTMATITYQNFFRLYTKLSGMTGTAKTEEEEFRKIYNMRVIQI
                     PTNRPVIREDAVDRVYARKEDKLNALVEEVKERHAKGQPILIGTSSVESSEEVSELLT
                     ANGLKHEVLNAKNHAREADIVALAGNKGQITIATNMAGRGTDIKINDEVKALGGLAVL
                     GTERHEARRIDNQLRGRSGRQGDPGFSRFYVSFDDLLMRRFSSDMMLKLVEGMGNEAL
                     ENKMFTNAITSAQKRIEGQNFDIRKSLLDYDDVLAKQRSIVYKKRDSIMYAGDIKDLV
                     QEQFTLAGLYLAKKAVPETGKDTLVNGVMLKQIMEPNYLPEGSINPSAYDESPPEEIG
                     EDLMLLMYRRYQLRRREWSEELADRVEREITLRAIDRNWTRHIDTMAHLREGIHLRSY
                     ANTNPLQDYVNEGYKLFGEAMDIIAVETVLNLLNAKINVAPPVEEGEEGTEENKETAE
                     KKNIKKEDKK"
     gene            2490..3600
                     /gene="prfB"
                     /locus_tag="PK612_01210"
     CDS             join(2490..2561,2563..3600)
                     /gene="prfB"
                     /locus_tag="PK612_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_132025324.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOH94766.1"
                     /translation="MEYKVALSKANELKRLLGKLSDSLDVASLKETIDKLTIEINNPD
                     FWNNQNKAKTIIKKLNAAKEKINIYSLVETTVESAFELLKDDTFAEDSEYLSDLAFEI
                     DNAQKEGELLINLLLFSGEYDENDATVSIHAGAGGTESHDWADMLYHMYARFCERQGF
                     SLKVVDYQAGNEAGIKSVTLIIEGNNAYGHLRSEKGVHRLVRISPFDANKRRHTSFAS
                     VNVVPIFDETGDIEINDSDLRVDTYRSGGAGGQNVNKVETAVRITHIPTGIVVSCQME
                     RSQLMNREIAMRMLKSELYQLELERQKAKVSAIEGEKKMIEWGSQIRSYTFAPYTLIK
                     DHRTNYEVGDIARVMDGDIAEFMYKYLEMEARSNG"
     gene            3593..4564
                     /locus_tag="PK612_01215"
     CDS             3593..4564
                     /locus_tag="PK612_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOH94767.1"
                     /translation="MAKQRIKDIFKEDWRTLLKNYSLIVVGSILLAFGTGVFLLPATL
                     NTGGLSGVGIIGEGLFGFDADLVVLVMTWAFFFISLLFLGWRFTIKSLVSSFVYPLAL
                     ILLMRLPAIATETVKLFGEGADVATKLIAGVFGGVFNGVGVALTFRGGGSTGGVDILV
                     VILNKYLRLSYSVMSFVIDGLIIAVGLFVLKDVVLSLIGILSAFVFAITLEFVFVGKK
                     HAMTASIISREKSAEINDFIQNEIGQGTTLVPVLGGYQKDEYMMVVVHFDRSNYARLI
                     NGIAKIDKKAFVSIEGSTVVLGGNFESLNPVKEGLVNIKKKDEKDKA"
BASE COUNT         1507 a          722 c         1024 g         1340 t
ORIGIN      
        1 gaaaggagat gtattaccta atcgaagtac cggggcatga ttagataata caagtgtata
       61 tgagttttat agaaaaaata ttccgctttg aacaaaggcg cctcaaaaaa atagaaaaaa
      121 cagccaaaaa agttattgct tatgaaaaaa cgatggaagc tttaactgat gatgaactaa
      181 aggctaaaac acccgaattt agagaacgac tcactaatgg ggaaacgctt gatgacttat
      241 taccagaagc cttcgctgtg gcacgcgaag cagcacgtag aactttagga caattccctt
      301 atcccgtcca aattatgggg gcaatcgtct tacatcatgg tgatgttgcg gagatgagaa
      361 ctggtgaagg taaaacttta actgcgacaa tggccgttta ccttaatgca ttatcaggca
      421 aaggtgtgca tgttgttact gttaacgaat atctcgctgc tcgtgacgcc gattggatgg
      481 gtcaaattta tcgctttctt ggtttaactg tgggtgttaa cctacgtgaa aaaacagcgc
      541 gtgaaaaacg tgaagaatat ttatgtgata ttacttacac tacaaatagt gaagttggtt
      601 ttgactatct tcgtgacaat atggctagca gtgttgatgg tcgtgtatta cgaggtttgg
      661 agtacgctat tgttgacgaa gctgactcga ttttaattga cgaatcacgc acaccgctca
      721 ttatttctgg tggcgctaaa gtacaagcat ctagttataa tgtcgccgaa cgtttcgtta
      781 aaacattacg cagagataag cactttgtcg ttgatattaa aacaaaaaca tgtaacttaa
      841 cggacgaagg taataaactc gcggaaagaa tgtttggtgt taataatatt tatgacccac
      901 aatatagtga cttagtgcac cggattcata atgcgcttaa agcaaattat gtcatgagtc
      961 gtgatgttga atatatggtt gacgcaaatc gtgaaattca acttattgac caatttactg
     1021 gacggattat gcccggtcgt gaatatagtg atgggttgca tcaagcaatt caagcgaaag
     1081 aaaatgtcac gattaaagaa gaaacagtga cgatggcgac gattacctat caaaacttct
     1141 tccgccttta cactaaacta agcgggatga caggtactgc taaaaccgaa gaagaagaat
     1201 tccgcaaaat ttacaatatg cgtgttattc aaatcccgac gaatcgccct gttattcgtg
     1261 aagacgctgt cgatcgagta tatgcacgta aagaagataa actcaatgca cttgtcgaag
     1321 aagttaaaga acgtcacgct aaaggacaac caattcttat tgggacaagc agcgttgaat
     1381 caagtgaaga agttagtgaa cttttaacag caaacggact caaacacgag gtacttaacg
     1441 ctaaaaacca cgcgcgtgaa gcagacattg ttgcgcttgc gggtaataaa ggccaaatta
     1501 caattgcaac gaacatggct ggtcgtggta ctgacattaa aattaatgat gaagttaaag
     1561 cacttggcgg ccttgcggta ttagggacag agcgacatga ggctcgccgt attgataacc
     1621 aattacgcgg ccggagtgga cggcaaggtg accctgggtt ttcacgcttc tatgtctcat
     1681 ttgatgactt actcatgcgt cgtttctcta gtgatatgat gcttaaactc gtcgaaggga
     1741 tgggaaacga agcactagaa aataaaatgt tcactaacgc tattactagt gcccaaaaac
     1801 gaattgaagg acaaaacttt gatattcgta aaagtttatt agattatgac gatgttttag
     1861 caaaacaacg ctctatcgtt tataagaaac gtgacagcat aatgtacgct ggtgatatta
     1921 aagacttagt gcaagaacag tttactcttg cgggtcttta tttagcaaaa aaagctgttc
     1981 cagaaactgg taaagatacg cttgttaatg gggtaatgtt aaaacaaatt atggaaccaa
     2041 actatttacc agaaggttcg attaaccctt cagcgtatga cgaatcacca ccagaagaaa
     2101 ttggtgaaga cttaatgctt ttaatgtatc gtcgttatca acttagaaga cgtgaatgga
     2161 gtgaagaact tgccgaccgc gttgaacgtg aaattacttt acgggcaatt gaccgcaact
     2221 ggacacggca tattgacaca atggcgcact tacgcgaagg tattcactta cgtagttacg
     2281 caaatactaa cccacttcaa gactatgtta atgaaggtta taagctattc ggtgaagcga
     2341 tggatattat tgctgttgaa acagttttaa atttattaaa cgcgaagatt aatgttgccc
     2401 cacctgttga agaaggtgaa gaaggcactg aagaaaataa agaaacagcc gaaaagaaaa
     2461 atataaaaaa ggaagataaa aaataaacga tggagtacaa agttgcctta agtaaagcta
     2521 atgaacttaa aagattacta ggtaagctta gtgactctct ttgatgtcgc tagtttaaaa
     2581 gaaacaattg ataaattaac gattgaaata aataatcctg acttttggaa taatcaaaat
     2641 aaggctaaaa caatcattaa aaaattaaat gctgcgaaag agaaaattaa tatatattca
     2701 ctagttgaaa caacagtgga aagtgccttt gaactactaa aagacgacac ttttgccgag
     2761 gatagtgagt atttaagtga tttagcattt gaaattgata atgcgcaaaa agaaggtgaa
     2821 ttacttataa atcttctgct tttcagcggg gaatatgatg aaaatgatgc aaccgtaagt
     2881 atccacgctg gtgcaggcgg gacggagtcg cacgactggg ccgatatgct ctatcatatg
     2941 tatgcacgct tttgtgaaag acaaggtttt tcgcttaaag ttgtcgatta ccaagcaggg
     3001 aacgaagccg gcataaaaag tgtaacactt attattgaag ggaataatgc ttatggtcat
     3061 ttgcgtagtg aaaaaggagt gcaccgctta gtgcgtatct caccttttga cgcgaataaa
     3121 agaagacata cttcatttgc gagtgttaat gttgtgccaa tttttgatga aactggtgat
     3181 attgaaatta atgatagtga tttaagggtt gacacttatc gcagtggtgg tgctggcggt
     3241 caaaatgtta acaaagttga gactgcagtg cgtattactc atatcccgac gggtatcgtt
     3301 gtatcgtgtc aaatggaacg cagtcaatta atgaatcgtg aaattgcgat gcggatgctt
     3361 aaaagtgaac tatatcaact agaattagaa cgacaaaaag caaaggtaag cgcgattgaa
     3421 ggtgaaaaga agatgattga atggggtagc caaataagat cttatacttt tgcaccctat
     3481 acccttatta aagatcaccg cacaaattat gaagtcggtg atattgcacg ggtgatggac
     3541 ggtgacatag cggaatttat gtataaatac cttgaaatgg aggcaagaag caatggctaa
     3601 acaaagaata aaagatatat ttaaagagga ttggagaact ttacttaaaa actatagttt
     3661 aattgttgtt ggatcaattt tactagcgtt tggtactggt gtttttcttt tacccgcaac
     3721 acttaatact ggggggttaa gtggtgtcgg cattattggt gaaggcttat ttggttttga
     3781 cgctgattta gtcgtacttg tgatgacttg ggctttcttc tttattagtt tattattctt
     3841 aggttggcgc tttacaatta aatcgcttgt tagttcattt gtttatcctt tagcgcttat
     3901 tttactcatg cgtttaccag caattgcaac tgaaaccgtt aaattgtttg gtgagggcgc
     3961 tgatgttgcc actaaactta ttgccggtgt ctttggtggt gtgtttaatg gggttggtgt
     4021 ggcattaact tttagaggtg gcggttcaac gggcggagtg gatattttag tagtaatttt
     4081 aaataaatat ttaagactaa gttatagtgt tatgtctttt gtgattgatg gcttaattat
     4141 tgccgttggg ttattcgtgc taaaagatgt tgtactctct ttaatcggta ttcttagtgc
     4201 atttgtgttt gcgattactc ttgaatttgt cttcgttggt aaaaagcatg cgatgactgc
     4261 ttcgattata agtcgtgaaa aaagtgcaga aattaatgat tttattcaaa acgaaattgg
     4321 tcaaggtaca accttagtgc cagttttagg tggttatcaa aaagatgagt acatgatggt
     4381 tgttgtccat tttgatcgta gtaattacgc tcgtttaata aacggtattg ctaaaatcga
     4441 taaaaaagct tttgtgagta ttgaagggtc aacagtagtg ttaggcggta attttgaaag
     4501 tttaaatcca gttaaagaag gcttagtaaa tattaaaaag aaagatgaaa aagacaaagc
     4561 ataaatttgt tttagaaacg acttttacgc cca
//
LOCUS       DAOSEX010000035         4565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_16989_length_4565_cov_3.476497, whole genome shotgun sequence.
ACCESSION   DAOSEX010000035 DAOSEX010000000
VERSION     DAOSEX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16989_length_4565_cov_3.476497"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1911)
                     /gene="pheT"
                     /locus_tag="PK612_01220"
     CDS             complement(<1..1911)
                     /gene="pheT"
                     /locus_tag="PK612_01220"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOH94768.1"
                     /translation="MKISRNALERYIDLSTLSDKEIADGLTFAGIETEDYYKLASGTN
                     LVIGHVLTCIKVEGSDHLSYCTVDVGRLGTREIICGAPNVRSGQKVIVALEGAILPVG
                     EIKRVTIKGHNSEGMICSLSELGVPSELQSHDDLVGIHVLSEDAPVGNTQVLEYLGLD
                     DTIFDIRPLANRSDMLALYNVVRELGAIFDLHYNIPEIEVNPSFKTKLKLEVNTPLVP
                     IFALTEVRNVAIKPSPQWMATFLRKHGHRPVNNIVDIGNYVMLVTGRPLHMYDLDKVK
                     DEHFIVSSDATTKFIALDEKEYNIVPGDQIISDKEKVLCLGGINGAKSSAVTNETKRI
                     AIEVAAFDTAHIRMSVVRHNLPSEASTRFGKGVNLNNTTEAMNLALSLIFDLVPGAEF
                     SNTVTVEKSLPAQPVIYFDKDKINKLLGTTFSEEVMLSTLNRLNITVNEDKSVNLPLY
                     RQDFFSLADLAEEIIRLLGFEHVGNEIPALFTTVGSYDEVQRKRYDIRKLLRSKGLYE
                     VLTYALTDEEKNKTFVYLNKDEPLKLLHPMTPVRSELRLNLLPSVIETLTYNASRQQS
                     DFALFEISNITSENYKGEHLAFAFYGNEVLSRELSKRPYDFFDAKGLIISVLEYLGIN
                     ESRYEFVLNPHKN"
     gene            complement(1921..2952)
                     /gene="pheS"
                     /locus_tag="PK612_01225"
     CDS             complement(1921..2952)
                     /gene="pheS"
                     /locus_tag="PK612_01225"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020060066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOH94769.1"
                     /translation="MDKRFESIKEEALSKINNAKSVKALNEVRNEYFAKSGLIATLMS
                     KIRELAPNERKDYGQAVNTLKVFLEEKLGAALREAQSNELSEKLESEKIDLTLPGRNL
                     ETAPQHPFHLVKDEICDIFIGMGYIIAEGDEVEEDLFNFTLLNMPENHPARDMQDTFY
                     INEKLLLRTHTSPVQARVMQKNKNQPLKMISPGKVFRRDDDDNTHSHQFGQVEGLVIG
                     EGISFSHLKATLDLFAKKMFGPKREIRLRSSYFPFTEPSVEVDVSCHVCNGKGCSTCK
                     GTGWIEILGAGMVNPNVLTMNGYDTEKFTGFAFGVGIERVAMLRYGIDDIRRFYQNDI
                     RFLKEFPKR"
     gene            complement(3270..4280)
                     /locus_tag="PK612_01230"
     CDS             complement(3270..4280)
                     /locus_tag="PK612_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOH94770.1"
                     /translation="MENNFYNEIKSILINARNKVYSTANFAMIEAYWNIGKKIIEKQG
                     GKDKAEYGLGLIKELSTQMTKDFGKGFTVTNLKYMRQFYLTFPNGHALRGELSWTHYR
                     SLMRVENEKARNFYLEEAIKSAWSTRQLDRQINSFFYERLLSSKNKEEVSQTIINLEK
                     GATPGDIIRDPYVLEFLGLVPNTDFYESDLEQALITHLQKFLLELGRGFSFVARQQRF
                     SFDGRHFRIDLVFYNYILKCFVLIDLKIGDLTHQDLGQMQMYVHYYEREMMNEGDNPP
                     IGIILCADKSDSVVKYTLPQDEKQIFASKYKLYLPSEEELLNELNKEYLSIENYIDEQ
                     KK"
BASE COUNT         1346 a          933 c          694 g         1592 t
ORIGIN      
        1 attcttatgt ggattaagca caaattcata acgcgattca ttaatcccta aatactctaa
       61 cacactaata atgagccctt tagcgtcaaa aaagtcatat ggtcttttac ttaattcacg
      121 acttaacact tcattaccat agaatgcaaa cgctaggtgt tcacctttat aattttctga
      181 agttatgtta ctaatttcaa aaagcgcaaa atcactttgt tgccgactag cgttataagt
      241 aagtgtttca ataacgcttg gtaaaagatt aagtcgtaac tcacttctga ctggtgtcat
      301 cgggtgcagt aattttaaag gttcatcttt atttaagtac acgaatgttt tatttttttc
      361 ttcatcagtt aaggcataag ttaacacttc ataaagccct ttactgcgta ataatttacg
      421 aatgtcataa cgctttcttt gtacttcgtc ataactccca acagtcgtaa ataatgcggg
      481 aatttcgtta ccgacatgtt caaagcctaa aaggcgaata atttcttcgg ctaagtcggc
      541 tagagaaaag aaatcttgcc ggtaaagtgg gagattaaca ctcttatctt cgttcaccgt
      601 aatatttaaa cggttaagtg tacttagcat tacttcttca ctaaaagtag tgcccagtaa
      661 cttattaatc ttatctttat caaagtaaat tacaggttgc gctggtaaac tcttttcgac
      721 tgtaactgta ttactaaatt cagcaccagg cactaagtca aagataagcg agagcgctaa
      781 attcattgct tcagttgtgt tatttaaatt aacaccttta ccaaagcgcg tacttgcttc
      841 acttggtaaa ttgtgacgca ctacactcat ccgaatatgc gccgtgtcaa aagctgctac
      901 ttcaatggca atgcgttttg tctcatttgt gactgctgaa cttttagcgc cgttaatccc
      961 gcctaaacat aatacttttt ctttgtcact aatgatttgg tcccctggaa caatgttata
     1021 ttctttctca tcaagcgcaa taaacttagt agtggcgtca ctactaacaa taaagtgttc
     1081 atcttttact ttatctaagt catacatatg taacggccga cctgtgacaa gcatcacata
     1141 gtttccaata tcaacaatat tattaacagg tctgtgtccg tgtttacgta agaaagttgc
     1201 catccactga ggggatggtt taattgccac attccttact tctgttaaag caaagattgg
     1261 cacaagcggt gtgttaactt ctagtttaag ttttgtctta aaagacggat taacttcaat
     1321 ttccggaata ttataatgta agtcaaaaat cgcaccaagc tcacggacaa cattatatag
     1381 cgcaagcata tcactcctgt tagcgagtgg tctaatatca aaaatagtgt cgtctaatcc
     1441 taaatattct aaaacttgtg tatttccaac tggtgcgtct tcacttagaa catggatacc
     1501 tactaaatcg tcgtgcgact gtaattcact aggcactcct aattcgctaa gtgaacaaat
     1561 catcccttca ctattatgtc ctttaatcgt gacgcgttta atttcaccga ctggtaaaat
     1621 tgctccttca agcgcgacaa taactttttg tccgcttcgc acattaggcg ccccgcatat
     1681 aatttcgcgt gttcctaatc taccaacatc aaccgtgcag taagaaaggt gatcactacc
     1741 ttcaacttta atgcaagtta aaacatgccc aattactaaa ttcgtaccac tagcgagttt
     1801 ataatagtct tcggtttcaa ttcccgcgaa agtgagtccg tccgcaattt ctttatcact
     1861 taaagtactt aaatcaatgt atcgttctaa tgcatttcgt gaaattttca taattattac
     1921 ctaccttttt gggaattcct ttaagaaacg gatgtcattt tgataaaagc ggcgaatgtc
     1981 atcaatcccg tagcggagca tcgctacgcg ttcgatcccg acaccaaagg caaatccggt
     2041 aaacttctcc gtatcgtagc cattcatcgt taacacatta ggattaacca ttcccgctcc
     2101 taagatttca atccaaccag tgcctttaca agtcgagcac cctttaccgt tacatacgtg
     2161 acaactaaca tcaacttcca cactaggttc agtaaacggg aaataacttg aacgtaagcg
     2221 tatttctcgt ttcggaccaa acattttttt cgcaaataag tcaagtgttg cttttaagtg
     2281 actaaaagaa atgccttccc caattactaa gccttcaact tgtccaaatt gatgggaatg
     2341 ggtattatca tcatcatctc tgcggaatac tttccccgga ctaatcattt ttagtggttg
     2401 atttttattt ttttgcatta cgcgtgcttg taccggtgaa gtatgtgttc taagtaataa
     2461 cttttcatta atatagaagg tatcttgcat atcacgcgct ggatggtttt ccggcatatt
     2521 taaaagagta aagttaaaga gatcttcttc aacttcgtcg ccttccgcaa taatgtagcc
     2581 catcccgata aagatatcgc aaatttcatc tttaacgagg tggaaggggt gttgcggcgc
     2641 tgtttctagg tttcttccag gtagtgttaa atcaattttt tcactttcta acttttcact
     2701 aagttcattg ctttgtgctt ccctaagtgc tgcgccaagt ttttcttcaa gaaacacttt
     2761 tagtgtattt acagcttgac cgtaatcctt ccgctcatta ggcgctagtt cgcgaatctt
     2821 actcataagc gtagcgatta agccgctttt agcaaaatat tcatttctta cttcatttaa
     2881 tgctttcaca ctcttagcgt tatttatttt acttaaagct tcttctttta tactttcaaa
     2941 ccttttatcc ataagaattt ctccttttat taaataaaaa agcgtcccca taggaacgac
     3001 tttcgccgtg gtaccatcct aattttccac tcttagttac gactttaacg acagtcaacg
     3061 ggtcatcaac cggctccaag gtgaattcgt gtctcttctc tagcggtgct tccaccatct
     3121 caccgtcgct atctaaagaa ctttaagaca ttactacttc ttttcaacgc cgcatatatt
     3181 ataccttaat ataatataaa ttggcaaaat tatgaatgtg gcaataggac aaaatgtaaa
     3241 cgactctttt gattagcggg gtaactcatt catttttttt gctcatcaat ataattttcg
     3301 attgataaat attctttatt taattcattt agtaattctt cttcactagg caaatataat
     3361 ttatatttag aggcaaatat ttgtttttca tcttggggta aagtgtattt aacaaccgaa
     3421 tcacttttat ccgcacataa gataatgcct attggtggat tatcaccctc attcatcatt
     3481 tctctttcat aataatgaac atacatctgc atttgtccta aatcttggtg tgttaagtct
     3541 ccaattttta aatcaattaa cacaaagcac tttaaaatat agttataaaa aacaagatca
     3601 atcctaaaat gccttccatc aaagctaaat ctttgttgcc ttgccacaaa ggaaaagcct
     3661 cggcctaatt ctaaaagaaa tttttgcaaa tgagttatca aggcttgttc taaatcactt
     3721 tcataaaaat cggtatttgg cactaaacct aaaaactcta atacatacgg atctcttatg
     3781 atgtctcctg gtgtcgcgcc tttctctaaa tttattattg tttgtgagac ttcttcttta
     3841 tttttactag ataagagtct ttcgtagaaa aaactgttta tttgtcgatc aagttgtctc
     3901 gtactccacg cagattttat tgcttcttct aaataaaagt tcctagcctt ttcattttca
     3961 acgcgcatta aactgcgata atgggtccag ctcaattcgc cacgcagtgc gtgaccattt
     4021 ggaaatgtta gataaaattg acgcatatat tttaaatttg tgactgtaaa tcctttaccg
     4081 aagtccttgg tcatttgcgt tgataattct ttaattaatc ctagtccata ttctgcttta
     4141 tccttcccac cttgtttctc aattattttt ttaccgatat tccaatatgc ctcaatcatc
     4201 gcaaaattag cagtactata aactttattt cttgcattta tcaatatgct tttaatttca
     4261 ttataaaaat tattttcgat agttaaaatc tctttctatt actatctaag tacaaccatt
     4321 ttgttctttt tataactaaa aaaattcttt tagtaataat tactttggtg tttactcgtt
     4381 aattagtggg tcaatataat ttatttattc gaaaataagg gcgggtaaaa ctaactttaa
     4441 ccatgtataa aaccgcgtag cattgtgtca ttctcaattc gctatgcgct gcgtagccaa
     4501 ttacatataa agtctttgca attattaaaa cactattctt ctttaatgta catttttcgt
     4561 attgt
//
LOCUS       DAOSEX010000036         4560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_17012_length_4560_cov_3.378468, whole genome shotgun sequence.
ACCESSION   DAOSEX010000036 DAOSEX010000000
VERSION     DAOSEX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17012_length_4560_cov_3.378468"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1923
                     /locus_tag="PK612_01235"
     CDS             85..1923
                     /locus_tag="PK612_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94771.1"
                     /translation="MSADVFREEEAVKKVTFGVWKQILKIILRSKKELALLLIFAVFM
                     SLAEASTIPLNAYAISNLIETYNESALLPFIVLSVTQIVVFAFGVFGFIYFGSKLETQ
                     VRYTLRKESFKNLQKLPFSYYDSTKQGWIMARMTSDTNRLARIVSWGILDLTWSGFFM
                     FFTIVVLFISEWRLALVFVSFLPILLFIAIIFRKKVLILNRKSRFHNSQLTGLFNESF
                     LGAKTTKSLAIESELSDEFTKEARVMKKVTLKAILYGAIFSSFLLAGSYTIVAFVMAL
                     GGYFALQGLVTMPILFMFIRAAMNTFEPILNLTNFINELQVAQASAERVLQLVNEKPS
                     IDDTQEVKDVYGDLFDKKKENWEEIKGDVEFKDVTFYYNPKEIILDKFNLKVKAGQTV
                     ALVGHTGSGKTTIVNLFARFYEPRAGEILIDGKDYRKRSLSWLHSRLGYVLQMPELFS
                     TSIKENIRYGNLEASDEEVIAASKAVGLDEFVNTLEKGYDTHVGEAGNLLSVGQKQLI
                     SFARAIIADPRILILDEATSSIDSEAEAKIQKATDELLKGRTSFVVAHRLSTIVNSDL
                     IVVLENGKIIEQGTHAELLGNRGHYFELYRNQFIAERREEKYLEID"
     gene            1926..2129
                     /locus_tag="PK612_01240"
     CDS             1926..2129
                     /locus_tag="PK612_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOH94772.1"
                     /translation="MTRTYIVEGMKNERSVRRIERALFRVDNISYEINLEKNTVVIHF
                     SNRVDDDKVIGAISRAGYRAMRL"
     gene            2276..2398
                     /locus_tag="PK612_01245"
     CDS             2276..2398
                     /locus_tag="PK612_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94773.1"
                     /translation="MVLGSEKKYLCLLCGMIIDNPDYCEYCGATSENIVPYEEE"
     gene            2498..3769
                     /locus_tag="PK612_01250"
     CDS             2498..3769
                     /locus_tag="PK612_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016359768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOH94774.1"
                     /translation="MKIRFINALILTMEENRPLFYGEVHVEGNRITYVGPSGDFNEPF
                     DRIINAEGNLLMPGFKNAHTHSPMTFLRSYADDLPLYDWLHKAVFPLEDKLEPGDQYV
                     LSKIAFAEYLTSGVTACFDMYYSPLETAQAAIDVGFRAALVGTVTKYKESVCEMKAAY
                     HTINKMHPLVSYHLGFHAEYTSTPEILKELAKAAHELKVPIWSHISETETEVKECIER
                     HGYTPAVYFDKLGLYNYGGGGFHGVHFSDEDFAVYKKRGLSIVTNPSSNTKLASGIAP
                     INKFLKEGINVAIGTDGPASNNALDFFREMWLVTGLAKLREKDAAVVDAYDVLKMATV
                     NGAEAMGLKDAKYLSVGSLADIIMLDMQQPNMQPVNNIEKNIVYAGSKVNVLLTMIDG
                     KILYEDGEFNLDEPISEIYRKAQAITERLKS"
     gene            3772..4434
                     /locus_tag="PK612_01255"
     CDS             3772..4434
                     /locus_tag="PK612_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOH94775.1"
                     /translation="MPNYNELKGNNLNFSIEKNKPNILKGKTLLFLGASFTYGHASFG
                     ESFVEYIEVRNDCTCIKEAVSGTTLVEHIEDSYITRLKKVPLGKKYDALLCQLSSNDV
                     RLKQEFGVIKESYYDTKTICGAIQYIAKYARDVLKCPVIFYTCPYFDKERYQKLVSIL
                     NEIATKMQFSVIDMYNDKNFNNISAETYALYMADPVHPTKAGYYYWLTPYIESHLFAF
                     FH"
     gene            4487..4558
                     /locus_tag="PK612_01260"
     tRNA            4487..4558
                     /locus_tag="PK612_01260"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4519..4521,aa:Met,seq:cat)
BASE COUNT         1492 a          691 c          934 g         1443 t
ORIGIN      
        1 gggttatatc aagtcttatg ggatattcaa ggagatttag aaacagagtt cttagaactt
       61 gttgctaagg agtgtgttta gagtatgagt gcagatgttt ttcgcgaaga agaagcagtt
      121 aaaaaagtaa ctttcggagt ttggaaacaa atcttaaaga ttattttaag aagtaaaaaa
      181 gaactagcgt tactcttaat ttttgcggtg tttatgtcac tcgcggaagc ttcaacaatt
      241 ccgcttaatg cttatgcaat tagtaatctt attgaaacat ataatgaaag tgccttactc
      301 ccctttattg tattaagtgt tacccaaatt gttgtctttg cctttggtgt ctttggcttt
      361 atttactttg gttcaaaact tgaaacacaa gtacgatata cattacgcaa agagtcattt
      421 aaaaatttac aaaaattacc atttagctat tatgactcga caaaacaggg ctggattatg
      481 gccaggatga caagtgatac aaaccgttta gcgcgcattg tctcatgggg aatcttagat
      541 ttaacttggt caggtttctt tatgtttttc acgattgttg tcttatttat tagtgaatgg
      601 cggctagctt tagtgtttgt gtcattttta ccaatcttac tttttattgc gattattttc
      661 cgtaagaaag ttttaatctt aaatcgtaaa tcacgtttcc ataatagtca gttaactggc
      721 ctttttaatg aatcattttt aggcgctaaa acaactaaga gcttagcaat tgaaagtgaa
      781 ttaagtgacg aatttacgaa agaagcgcgc gtaatgaaaa aagtgacgtt aaaggcgatt
      841 ctttatggcg ctattttctc gagtttcctt ttagcgggca gttacacaat tgttgccttt
      901 gtcatggcgc ttggtgggta ctttgccctc caaggcttag tgacgatgcc gattctcttt
      961 atgtttattc gtgcggcaat gaatactttt gaacctatct taaacttaac gaactttatt
     1021 aatgaactac aagtcgcgca agctagtgct gaacgtgttt tacaattagt caacgaaaag
     1081 ccgtcgattg atgatacgca agaagttaaa gatgtgtatg gtgatttatt tgataagaaa
     1141 aaagaaaatt gggaagaaat taaaggtgat gttgaattta aagatgtgac attctattac
     1201 aatccaaaag aaattattct cgataaattt aatttaaaag ttaaagctgg gcaaacagta
     1261 gcacttgttg gtcataccgg cagcgggaaa acaacgattg ttaatctttt cgcaagattc
     1321 tacgagccgc gcgctggtga aatattaatt gatggtaaag attatcgaaa gcgtagtcta
     1381 tcatggctcc atagccgctt aggatatgtt ttacaaatgc ccgagctttt ctccacatcg
     1441 attaaagaaa acattcgcta tggtaattta gaggcaagtg atgaagaagt cattgctgcg
     1501 agtaaagcag tcggccttga tgaatttgtg aataccttag aaaagggata cgacacgcat
     1561 gttggtgaag ctggtaacct tttatcagtg ggccaaaagc aactcatctc ttttgcgcga
     1621 gcaattattg ctgatccacg aattttaatt cttgacgaag cgactagttc gattgatagc
     1681 gaagctgaag caaaaatcca aaaagccact gatgaactct taaaaggccg aacaagtttt
     1741 gttgttgcgc accgcttaag cacgattgtt aatagtgatt taatcgttgt tttagaaaac
     1801 ggtaagatta ttgagcaagg tactcacgct gaattactag gtaatcgcgg gcattacttt
     1861 gaattatatc gaaatcaatt tatcgcggaa cgccgtgaag aaaaatatct ggagattgat
     1921 taatagtgac acggacttat attgttgaag ggatgaaaaa cgaacgatcc gtacgtcgta
     1981 ttgaacgtgc gttatttaga gtggataata taagttacga aataaattta gaaaaaaata
     2041 ctgttgtgat ccactttagt aatagagtag atgatgacaa agttatcggt gcaattagtc
     2101 gcgctgggta tcgggcaatg cgattataat taatgaaaat ttaaaatttt gtaataaaaa
     2161 acttgattta aacacattta taaataaaga aaattagttg taaattaatg catcagcggg
     2221 tttacttcta ataattataa gatataatag aatatggaag gaagaggaaa taaatatggt
     2281 attaggttct gaaaagaaat atttatgttt attatgtggg atgattattg ataacccaga
     2341 ttattgtgaa tactgtggtg caacatcaga aaacatcgtt ccttacgaag aagaataaat
     2401 attaaataaa catataaaaa agcatggggc aacccatgtt ttttattatt aataaaaaca
     2461 tgctaaaatt aagtatctaa taaatgagga aaacattatg aaaatacgtt ttattaacgc
     2521 acttattctt acaatggaag aaaatcgtcc gcttttttat ggtgaagtcc atgttgaagg
     2581 taatagaata acttatgttg ggccaagtgg tgactttaat gaaccgtttg accgtattat
     2641 taacgctgaa ggtaatttac tcatgcctgg ctttaaaaac gcccatacgc attcaccgat
     2701 gacattttta cggagttacg ctgatgactt accgctttat gactggttac ataaggccgt
     2761 atttccgctt gaagataaac ttgagcctgg cgatcaatat gtcttaagta aaattgcttt
     2821 tgcggaatac ttaacaagtg gcgtgacggc atgttttgat atgtattatt caccacttga
     2881 aacagcgcaa gcggcaattg atgttggttt tagagcggca cttgtgggca ctgttacaaa
     2941 atacaaagaa agtgtttgcg aaatgaaagc agcatatcac acgattaata aaatgcatcc
     3001 gcttgttagt tatcatcttg ggttccacgc tgaatataca agcacgcccg aaatattaaa
     3061 agaattagcg aaagctgctc acgaattaaa agtcccaatt tggagtcata ttagtgaaac
     3121 agaaacggaa gtaaaagaat gtattgaacg ccacggctac accccagcag tgtattttga
     3181 taaattaggt ttatataatt acggtggtgg cggtttccac ggagtgcact ttagtgatga
     3241 agactttgcc gtgtataaaa agcgcggttt aagtattgtg accaatccta gttcaaatac
     3301 taaacttgca agcggaattg ccccgattaa caaattcctt aaagaaggga ttaatgttgc
     3361 aattggcact gatgggcccg ctagtaataa cgcgcttgat ttcttccgtg aaatgtggct
     3421 tgttactggt ttagcgaagt tacgtgaaaa agacgcggcg gttgttgatg cttatgatgt
     3481 ccttaaaatg gctactgtaa atggtgctga agcaatggga ttaaaagacg ctaaatattt
     3541 aagtgttggc agtctagcgg acatcattat gcttgatatg cagcaaccga atatgcaacc
     3601 tgttaataat attgaaaaaa atattgtgta cgctggtagt aaggttaatg tattattaac
     3661 gatgattgat ggcaagattt tatatgaaga cggtgaattt aatcttgatg aacctattag
     3721 tgaaatttat cgtaaagcgc aagctattac tgaacggtta aaaagttaaa tttgccaaat
     3781 tataatgaac ttaaaggcaa taatttaaat tttagtattg agaaaaataa gccaaatatt
     3841 ttaaaaggta aaacattatt atttcttggt gcgtctttta cttatggtca cgcttcgttt
     3901 ggtgaatcgt ttgtcgaata tattgaagtg cggaatgatt gtacttgtat taaagaagcc
     3961 gtgagcggca caacacttgt ggagcatatt gaagatagtt atattactag gctaaaaaaa
     4021 gtaccgttag gcaagaagta tgacgcatta ctttgtcagc tctcaagtaa tgatgtgcgg
     4081 cttaaacaag aatttggtgt tattaaagaa agttattacg atacgaaaac aatatgtggc
     4141 gctattcaat atattgcaaa gtacgctcgt gatgtgctta agtgtcctgt tattttttac
     4201 acttgtcctt attttgataa ggagcggtat caaaaattag taagtatact taacgaaatt
     4261 gctacgaaaa tgcagtttag tgtaattgat atgtataatg acaaaaactt caataatatt
     4321 tccgcggaga catacgcttt atatatggct gatcctgttc atccaactaa agcgggatat
     4381 tattattggc ttactcctta catagaaagc cacttatttg ccttttttca ctaaattaat
     4441 tagataattt gcattaatta ataatcatgt tataatacta atacatcgcg gagtagagca
     4501 gtggtagctc gtcgggccca taacccggag gtcggaggtt caaatcctcc ctccgcaacc
//
LOCUS       DAOSEX010000037         4422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_17827_length_4422_cov_2.908862, whole genome shotgun sequence.
ACCESSION   DAOSEX010000037 DAOSEX010000000
VERSION     DAOSEX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17827_length_4422_cov_2.908862"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PK612_01265"
     CDS             <1..968
                     /locus_tag="PK612_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOH94776.1"
                     /translation="KANRKRSTSADVLNDLKDKHPIIPAILEYRKYQKIISTYIDGFI
                     PHIHRDGKVHTTFNQALTTTGRLSSNNPNLQNISIRHEEGREIRKAFNAGDKDVLAID
                     YSQIELRLLAAIANVPSLIDIFNTDRDIHSETAIALFPLEDLNYARRKAKAVNFGIVY
                     GISDFGLSEQLETTVKDARAIIQSFFKTYPEVWTYRQEVINELIKDGYVKTLTGRKRY
                     IPQINDPNYQTREFAKRAAMNAPLQGSAADLIKMAMIKVDEFLTKNKCKTKLILQVHD
                     ELIFALDQDEHEIVDDLVNIMENVLDLPVKLKVEVKTGKTWFEAS"
     gene            974..2347
                     /gene="mutM"
                     /locus_tag="PK612_01270"
     CDS             974..2347
                     /gene="mutM"
                     /locus_tag="PK612_01270"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOH94777.1"
                     /translation="MPELPEVETVKKILNKVIVGAKITDVIIHKENIITGDPHTFKKT
                     LIGETFSEVKRIGKYLIFTFESDLIMLSHLRMEGKYIEVLPGAEDSRYARVIFVLNDG
                     RRLCYDDSRQFGTLELSTKDTYLQTKSMQNVGREPFDIDPIDYFNKISNRTTPIKQTL
                     LDQKLMSGLGNIYVDEVLYLSKIHPETPTNMLGLMAINLLIENSIKVLNAAIKKGGTT
                     VRSYSPAEGVHGNFQQELNAYGKAGERCPVCNFKFKKIFVGGRGTTYCPSCQKNPAVP
                     LTVAITGQKASGKTLIANYLRKNGHKVIDTDKLAKELYKDIKITTDLEKHLGVNLHDN
                     TTFNLQYLRNYLVENENEIKKLNDFIHPLVKKEIIEIIKTSNEAILYFEVPLLFSHKI
                     YELFNFMIGVEVSVEKQLKNLEKRGDTHTINADEAYMKNRHKLDLIIVNDGSEQDLIE
                     KFKAFKV"
     gene            complement(2337..3215)
                     /locus_tag="PK612_01275"
     CDS             complement(2337..3215)
                     /locus_tag="PK612_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94778.1"
                     /translation="MKKVELDIDTTNVEEIRQEMYDKIMSHKSFVKLLEEKGATPEII
                     KNNVGLLNDYLESYLSVEKCRKAGHCVSDEKHHALTIQIDAPFVTVERHLCPVYLESQ
                     SLAMRFIVRDFPENYLDLRVEDLPARRGFAAFIRELMKVLKGDIYFIYAYGTAGIGKL
                     KSTIPFVTKMLEDDKNKTAGVVNFPAFVRASIADYYDKKDIVDSYLQHYIDLDVLVLD
                     NFGNEEVNKIVIESIIFPLLAARVRQKKPTIILAGLSTNDLRSLYVGRSVRGHQIVDL
                     IKANIKEEIYLQGTKL"
     gene            complement(3212..>4422)
                     /locus_tag="PK612_01280"
     CDS             complement(3212..>4422)
                     /locus_tag="PK612_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94779.1"
                     /translation="VRGYVTSLHLEVLDILYQPIMGFAATALYRYLFTMRDFKKGDSL
                     SFTLIHDHFGFSYDQISIAFSKLEGLGLMRSFYVEYNEFSEYVLELYAPKDPAAFSQD
                     QIFMNLLNDVLGPRHTQDLLSLFTLDRETLNKEEVTSTFAEIYADRIFDIKGDDLYKG
                     EPLENVVGNIKFEFDRAAFVNILTRERKILPSALSAEELKKAEQMAGLYNLTEVATAE
                     RVGDFYDALQPFGARVDFVAMNKHLQELVKYPVLSKPLRRPVQELAGESQKIKRINLM
                     ETYSPVEFLQYLNEGTKIAPADYNILTTLSHDYNLLSPVINALVYYTLYHNNNELIRA
                     LVEKNASLLARNKVTTALDALDTLERPIKKRGRAPKKAPILNDDKKEEATPQVDDLDD
                     PIWDELKKLK"
BASE COUNT         1506 a          785 c          755 g         1376 t
ORIGIN      
        1 ccaaggctaa tcgtaaacgc tccacttccg ctgatgtgtt aaatgactta aaagataagc
       61 atccaattat tcctgctatt cttgaatacc gcaaatatca aaagattatt tcaacttata
      121 ttgatggctt tattccccat attcatcgtg acgggaaagt tcatactacc tttaaccaag
      181 ccttaacgac aacgggtcga ctaagcagta ataatccaaa cttacaaaat attagcattc
      241 gccatgaaga gggacgcgag ataagaaaag catttaatgc cggagataag gatgttttag
      301 caattgacta tagtcaaatt gagttacgct tacttgccgc tatcgctaat gttcctagtc
      361 ttattgatat ttttaacact gaccgcgata ttcatagtga aaccgcaata gcactatttc
      421 cgcttgaaga cttaaattac gcaagacgga aagcaaaagc cgttaacttt ggcatagtgt
      481 atggcattag tgattttggc ttaagcgaac aacttgaaac aacagtaaaa gacgcccgcg
      541 ctatcattca gtccttcttc aagacttatc ccgaggtatg gacttataga caagaagtaa
      601 ttaatgagtt aattaaggat ggttacgtta aaacattaac tggtcgtaaa cgctatatcc
      661 cgcaaattaa tgatcctaac tatcaaacac gcgaatttgc aaagcgtgct gcgatgaatg
      721 ctccgcttca aggcagcgct gccgatttaa ttaaaatggc aatgattaaa gtcgacgaat
      781 ttctaacaaa gaataagtgt aaaacaaaac taattttaca agtccatgac gaacttatct
      841 ttgcgcttga tcaagatgaa catgaaattg tcgacgattt agtgaatatt atggaaaatg
      901 tccttgattt accagtaaaa ttaaaagtgg aagttaaaac cggaaaaaca tggtttgaag
      961 cgagttaata attatgccag aactaccaga agttgaaaca gttaaaaaaa tcttgaataa
     1021 agtcattgtt ggggcaaaaa ttactgatgt tattattcat aaagaaaaca tcatcactgg
     1081 ggacccacac acttttaaaa agacacttat tggtgaaact tttagtgaag taaaacgcat
     1141 tggtaaatac ttaatcttta cctttgaaag tgatttaatt atgcttagtc acttacggat
     1201 ggaaggtaag tatattgaag ttttaccggg tgctgaagat tcgcgttacg ctcgtgtaat
     1261 ttttgtgtta aacgatggca ggcgcctatg ttatgacgat tcacggcaat ttggtacgct
     1321 tgaattatca actaaagaca cttacttaca aacaaaatca atgcaaaatg taggaagaga
     1381 accctttgat attgatccga tcgattattt taataaaata agtaaccgca ctacaccaat
     1441 taaacaaact ttacttgacc aaaagctgat gagcgggctc ggtaatattt atgttgatga
     1501 agtattatac ttaagtaaaa ttcatccaga aacaccaacg aatatgttag gacttatggc
     1561 gataaattta cttatcgaaa atagtattaa agtacttaac gcggccataa aaaaaggcgg
     1621 cacaaccgta agaagttata gtcctgctga aggagtgcac ggtaacttcc agcaagaatt
     1681 aaatgcttat ggtaaggcgg gtgaaaggtg tcctgtttgt aattttaaat tcaagaaaat
     1741 atttgttggt gggcgcggta caacttattg tccaagttgt caaaaaaatc ccgctgtgcc
     1801 attaactgtc gccattactg gtcaaaaagc aagtgggaaa acacttatcg ctaattactt
     1861 acggaaaaat ggacacaaag ttattgacac tgataaactc gctaaagagc tttataagga
     1921 tattaaaatt acaactgatt tagaaaaaca tctcggcgtt aatttacatg ataacactac
     1981 ttttaacctt caatatttac gaaattatct agtggaaaat gaaaatgaaa taaaaaaact
     2041 aaatgacttt attcatccgc ttgtaaaaaa agaaattatt gaaattatta agacgagtaa
     2101 tgaagcgatt ctttattttg aagtaccgct tcttttctcg cataaaatat atgaattatt
     2161 taattttatg attggggttg aagtaagcgt tgaaaagcaa cttaagaatt tagaaaagcg
     2221 cggagatacg catacaatta acgctgatga agcatatatg aaaaaccgtc ataaactaga
     2281 cttaatcatc gttaatgacg gtagtgaaca agatttaatc gaaaagttta aagcttttaa
     2341 agtttagtgc cttgtaaata aatttcttct ttaatattgg ctttaattaa gtcaacaatt
     2401 tgatggccac ggacactgcg accaacataa agacttcgta agtcattcgt gcttagaccc
     2461 gctaaaataa tagtgggctt tttttgtctg actctcgctg ctagaagtgg aaaaataatt
     2521 gattcaatga caatcttatt aacttcttca ttaccgaagt tatcaagcac taaaacatct
     2581 aaatcaatat aatgttgtaa ataagaatca acgatatctt ttttatcgta gtaatcagca
     2641 attgatgctc taacaaaagc agggaaattg acaactcccg ctgttttatt tttatcatct
     2701 tcaagcattt tagtcacaaa cggaatcgtt gattttaatt taccaatccc cgctgtacca
     2761 taagcatata taaagtagat atcacctttt aaaactttca taagttcacg aataaaagct
     2821 gcaaagcctc gtctagcggg taagtcttcg actcttaaat ccaaataatt ttcgggaaaa
     2881 tcacgaacaa taaagcgcat cgctaatgat tgactttcta gataaactgg gcataaatgt
     2941 ctttccactg tcacaaacgg cgcgtcaatt tgaatagtga gtgcatgatg cttttcatca
     3001 ctaacgcagt gaccagcttt acgacatttt tctacactta agtaactttc taaataatca
     3061 tttaaaagtc caacattgtt cttaattatt tctggagttg cacctttttc ttctaaaagt
     3121 ttaacgaaag atttatgact cataattttg tcatacattt cttggcgtat ttcttcaaca
     3181 tttgtggtgt caatatctaa ttctactttt ttcattttaa cttcttcaat tcatcccaaa
     3241 ttggatcatc aagatcatca acttgcggtg ttgcttcttc tttcttgtca tcgttaagga
     3301 ttggcgcttt tttaggtgcg cgtccgcgtt tttttatcgg ccgttcaagt gtatcaaggg
     3361 catcaagcgc ggtagttact ttgtttctcg ctagtaaact agcgtttttc tccactaaag
     3421 cacggataag ttcattgtta ttatgataaa gcgtgtaata aacaagggca ttaattactg
     3481 gtgataataa attataatca tgacttagcg tcgttaaaat attataatca gcaggagcaa
     3541 ttttcgtccc ttcatttaag tattgtaaaa attcaacggg actatatgtt tccattaaat
     3601 taatccgctt aattttttga ctttcacccg caagttcttg aactggacgg cgtaatggtt
     3661 tacttaaaac ggggtacttc acaagttctt gcaagtgctt attcatcgcc acaaaatcga
     3721 ctctagcgcc aaagggttgt agagcgtcat aaaaatcacc gacacgctcc gctgttgcta
     3781 cttccgtcaa attatataaa ccagccattt gctcagcttt tttaagctct tcagcactta
     3841 gtgctgaggg caaaatttta cgttcacgcg ttaagatatt aacaaaagca gcgcgatcaa
     3901 attcaaattt aatattaccg acaacatttt ctaatggttc acccttatat aggtcatcac
     3961 ctttaatatc gaaaatacga tcagcgtaaa tttcggcaaa agttgatgta acttcttctt
     4021 tatttaatgt ttcacgatcg agcgtaaata aagataataa gtcttgtgta tggcgcggac
     4081 caagcacatc atttaataaa ttcataaaga tttgatcttg actaaacgct gctgggtctt
     4141 ttggtgcata taattctaag acatattcac taaattcatt atattcaaca taaaatgacc
     4201 gcattaaacc aagaccttct aatttactaa aggcgatact aatttggtca taactaaacc
     4261 caaaatgatc atgaattaaa gtaaacgata gcgaatcacc ttttttaaaa tcacgcattg
     4321 taaataaata gcgatataag gctgtcgcgg caaagcccat gattggttga tataaaatat
     4381 ctaaaacttc aagatgtaac gatgttacat aaccgcgaac tt
//
LOCUS       DAOSEX010000038         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_18807_length_4249_cov_3.198140, whole genome shotgun sequence.
ACCESSION   DAOSEX010000038 DAOSEX010000000
VERSION     DAOSEX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18807_length_4249_cov_3.198140"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="PK612_01285"
     CDS             complement(<1..127)
                     /locus_tag="PK612_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HOH94780.1"
                     /translation="MKILVTGAKGFVGRNLCAQLHNIKEGKARFYGDLVVDEVYEY"
     gene            complement(139..693)
                     /locus_tag="PK612_01290"
     CDS             complement(139..693)
                     /locus_tag="PK612_01290"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOH94781.1"
                     /translation="MAKNIMVVTGSVRKTGNSIMLANAFVKGARNNGNAIHKVEASHR
                     NFSPFRDKENAWRKGKPSLVEDDFNVLAPYLHVSDVLVLASPVYFGGFSGAIKVFIDN
                     LYAYVVEHRKRNLRFTHAILLAVARDDNPDTFVGITHEFKELCRQLGIEKMDIITVPG
                     VAERGDIVGHEALDRARDLGKTIS"
     gene            complement(764..1693)
                     /locus_tag="PK612_01295"
     CDS             complement(764..1693)
                     /locus_tag="PK612_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH94782.1"
                     /translation="MNILMILTPKSEVIYLSTKMRVRDAMEVLKKARFNSVPLLDESG
                     HYVGTVTEGDILWHLDQNGYEDSLDMRLKNVKRIRDYAPVSVDEEIDELIHKALGQNF
                     IPILDDRKYFIGIVTRKNLISTYIKKVDDNKEEVVHKNEVLDNLYKRRSIRRFKEGIT
                     IPQEIIDEIVKVSLVTPTAMNRRPHEIMLVDDPQVIKTLSELHQRGGQFEKAPYLILI
                     LNDDEKEPNAYLANSNCSALLISLLLAVESFDNLGGFWVSTRYPEYNRKMLNAVGVPE
                     RFSLYGMVAFGVKGEHKPANEPLNPEKIHRNKW"
     gene            1813..2637
                     /gene="nagB"
                     /locus_tag="PK612_01300"
     CDS             1813..2637
                     /gene="nagB"
                     /locus_tag="PK612_01300"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOH94783.1"
                     /translation="MTIFIRDNYNEMSKAAAKIIAKQIKEKPDSVIGLATGFTPIGTY
                     RELIRMHKEEGLSFKGVTAFTLYEFLGEGFDIIKPYGLDKSSARYMHEEFFKHVDIDK
                     KNIHYFDGFTDDPRRTCQEYEEKIKAAGGIDLQLLGIGRDGHWGYNEPGSSLGSRSHV
                     VALTKQTIDDNYEDFYRKVGITKDEMPHFALTMGVGTILDARKLILVASGAKKAEIVQ
                     KALEGPITAQITSTAIQLYAGEATIILDADAASMLSNIEHIKHTEAMSKKYGFRAE"
     gene            complement(2666..4093)
                     /locus_tag="PK612_01305"
     CDS             complement(2666..4093)
                     /locus_tag="PK612_01305"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOH94784.1"
                     /translation="MATILIVGLTFFFLEQSIITDVTPGYYFDPLTLSNQMMVTGIIV
                     TLVSIFSFFVIRKIRLSVNGIILCIVLTIVFTMALVNLLIMPKEQLLSIRFLDGTYHD
                     INFVLPTRMRLTAIMTLVVNFSFIFFVVLVLPNHVSFLKLVLFFTIAIVLIAFFAIGY
                     SLATEWEKYVAIVKEGPGSTYQVPASFFNNRNPYASFLLNAQVMLFFLYYKNLDKKRR
                     FFYILLQIPLVLAINFTYSKTNLLLANILLIIAFLFHLVRLLGRKKFIRFGIEVFLAF
                     LLVIGFIVFRYSPKLETTNISLFLHRTLPDEMFERVPGSFDSRKKLWYYAVSLMVANP
                     IIFVFGHGPHLSRYFYHLRMQIEIEGVMTPGFGDYHNGLVEVMHTFGASGLIIYALVF
                     LFVTILLIRRARTNRSLTFFVLLSMGVFILRSQMESLAVLLFKSEGIMASLTFVLPAL
                     YLLRKSHECGFSKELKVKKTRPEEA"
BASE COUNT         1450 a          783 c          688 g         1328 t
ORIGIN      
        1 catattcata gacttcatct acgactaaat caccgtaaaa gcgagctttt ccttctttga
       61 tgttgtgtaa ttgtgcacac aagtttcttc caacaaaacc tttagcacca gtaactaata
      121 ttttcataaa actatttcct aactaattgt ttttcctaaa tcccgcgcac ggtcaagtgc
      181 ttcatgccca acaatatcac cacgttcagc tacaccaggt acagtaataa tatccatttt
      241 ttcaatacct aactgccgac ataattcttt aaattcatgg gtaataccaa caaatgtatc
      301 gggattatcg tcacgcgcta ccgcaaggag tattgcatgc gtaaagcgta agttgcgttt
      361 acgatgttcg acaacataag cataaagatt atcaatgaat actttgattg cgccgctaaa
      421 gccaccaaag taaacaggtg aagcaagcac taaaacatca ctgacatgta aatatggggc
      481 aaggacatta aaatcgtctt cgactaaact tggtttacct ttccgccatg cattttcttt
      541 atcgcgaaat ggtgaaaaat tacgatgcga agcttcaacc ttgtgaatcg cattaccatt
      601 atttcgcgcg cctttaacaa aagcatttgc aagcataatt gagttgcctg ttttccggac
      661 gctgcctgta actaccataa tgttttttgc catattgcct ccataaataa ttatatcata
      721 aaattgttat attgtgctta aaatcgtgcc ttttattaag tttttaccac ttgttacgat
      781 gaattttttc tgggttaagt ggttcatttg ccggtttatg ttcacccttg actccaaagg
      841 caaccatccc gtagagacta aagcgttctg gtacaccaac agcgtttagc atcttcctgt
      901 tatattctgg ataacgggta ctaacccaga acccacctaa attatcaaaa ctttcaacag
      961 cgagtaataa gctaataagt aaagcactgc agttactatt tgcaagataa gcatttggtt
     1021 ctttttcatc atcatttaat attaatatta aatatggagc tttttcaaat tggccgcctc
     1081 tttgatgtaa ttcgctaagt gttttgataa cttgtggatc atcgactaac attatttcat
     1141 gtgggcgtcg attcattgcc gttggggtta ctaaactaac tttaacaatt tcatcgatta
     1201 tttcttgtgg aatagttatg ccttctttaa agcgtctaat acttcttctt ttatataaat
     1261 tgtcgagcac ttcattttta tgaacaactt cttctttatt atcatcaacc tttttaatat
     1321 aagtgctaat taagttcttc cgtgtcacaa taccaataaa gtactttcga tcatcaagaa
     1381 ttggaataaa gttttgaccg agtgctttat gaattaattc atcaatctct tcgtccactg
     1441 atacaggagc gtaatcccta atgcgtttaa cgttttttaa gcgcatatca agtgaatctt
     1501 cataaccatt ttgatcaaga tgccataaga tgtcaccttc agtgacagtg ccaacataat
     1561 gaccactttc atcaagtaaa ggtacactat taaatcgtgc tttctttaac acttccatcg
     1621 catctcttac acgcatttta gttgataaat atatgacttc actcttcggg gttagtatca
     1681 ttaaaatgtt catgctccac ctctttctat actataatta tataataaaa ccgctttcac
     1741 tgtggtaaaa aaaggaactt tgttgtatta tatggtgtaa ctatatgtta gactatttat
     1801 gaggagaata ttatgactat ttttatacgt gacaattata acgagatgtc aaaagcagca
     1861 gcaaaaataa ttgctaagca aattaaagaa aaacctgatt cagttattgg ccttgcgaca
     1921 ggctttacac caattggcac ttatcgtgaa ctaattcgga tgcataaaga agaaggttta
     1981 agttttaaag gagtaacagc ttttaccctt tatgaattct taggtgaagg ttttgatatt
     2041 attaagcctt acggattaga taaatcaagc gctcgttaca tgcatgaaga atttttcaaa
     2101 catgtcgaca tcgataagaa aaatatccat tattttgacg gatttaccga tgatccacgt
     2161 cgcacctgtc aagaatatga agaaaaaatt aaagcagcag gcggaattga cttacaactc
     2221 ttaggtatcg gccgtgatgg ccactggggt tataacgaac caggctcaag tttaggtagt
     2281 cgtagccatg ttgttgcttt aactaaacaa acaattgatg ataactatga agacttttat
     2341 cggaaagtag ggattactaa agatgaaatg ccgcactttg ccttaacaat gggcgttggc
     2401 acaattttag atgcaagaaa attaatcctt gtcgctagcg gagctaaaaa agcagaaatt
     2461 gtacaaaaag cacttgaagg accaatcacc gcccaaatta cttctactgc tattcagtta
     2521 tatgctggcg aagcaacaat tattttagac gctgatgccg caagtatgtt atcaaatatt
     2581 gaacacatta aacataccga agcaatgagt aaaaaatacg gcttccgtgc cgaataatac
     2641 ttattaatca tattttaaga aatgactagg cctcttctgg cctagttttt ttaactttta
     2701 attctttact aaagccacat tcatgcgact ttcttaataa ataaagcgca ggtaatacga
     2761 atgttagtga agccataata ccttcgcttt taaaaaggag aactgctaaa gattccattt
     2821 gactccgtaa aataaagacg cccatactaa gcaagacaaa gaaagttaaa ctgcgatttg
     2881 tccttgctct tctaattaat aatatcgtta caaagagaaa aacaagggca tatattatta
     2941 atccgcttgc gccaaaagta tgcattactt cgactaagcc attatgataa tcaccaaagc
     3001 ccggtgtcat tactccttct atttcaattt gcattcgtaa gtgatagaaa tagcgcgaaa
     3061 gatgtggacc gtgaccaaag acaaatatta tcggattagc gaccataaga ctaactgcgt
     3121 aataccaaag tttcttccgt gaatcaaaag agccaggaac cctttcaaac atttcatcag
     3181 ggagtgtccg atgtaaaaat aaagaaatat tcgtcgtttc taattttggc gaataacgaa
     3241 agacaataaa tccaattacg agtaaaaatg ctaaaaacac ttcaatacca aagcgaataa
     3301 acttcttgcg acctaataac ctaaccagat gaaataaaaa agcaataata agcagtatat
     3361 tagctaataa taaatttgtt ttagaataag tgaagttaat agctaaaaca agcggaattt
     3421 gcagtaaaat ataaaagaaa cgtcgttttt tatcaagatt tttataatag aggaaaaaga
     3481 gcattacctg cgcattcagc aaaaacgacg cataaggatt tctgttatta aaaaaactag
     3541 cgggcacttg gtaagtgctc cctggaccct cttttacaat tgcaacatat ttttcccatt
     3601 cagtagcaag actgtaccca atagcaaaaa aagcgattaa gacaattgct atagtaaaga
     3661 aaagcactaa ttttaaaaat gacacatgat ttggtaacac aagcacgaca aagaaaataa
     3721 aactaaaatt cacaacaagc gtcataatcg ctgttaaacg catccttgtt ggtaacacaa
     3781 agtttatatc gtgatatgtt ccatctaaaa agcgaattga aagcaattgt tcttttggca
     3841 ttattagcaa gttaacaagt gccatagtaa atactatagt aagtacaata cacaggataa
     3901 taccgtttac actaagacga atcttacgga taacgaaaaa agaaaaaata ctaacgagtg
     3961 ttacaatgat tcctgttacc atcatttgat tagaaagtgt tagcggatca aagtaatatc
     4021 caggagtaac atccgtaatt attgactgtt ctaaaaagaa gaaagttaag cccacaatta
     4081 gaattgtggc caaaaagtat ataaacagat ttgctctttc cctgacttta ctcatttttt
     4141 aattatacta ctatttgttc ttaaatgtta atgcagataa ttgtgcagtt aaaataacac
     4201 acattttatg tgtgtatttt atttgtgttt ttttgctata attttcttt
//
LOCUS       DAOSEX010000039         4231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_18936_length_4231_cov_2.723659, whole genome shotgun sequence.
ACCESSION   DAOSEX010000039 DAOSEX010000000
VERSION     DAOSEX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18936_length_4231_cov_2.723659"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PK612_01310"
     CDS             complement(<1..414)
                     /locus_tag="PK612_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94785.1"
                     /translation="MPYYIIESKTIRDAGNWLNVKDIYGLSDKNNTVSGYATGYVPTL
                     QVIRPDGNGATYLANNDISPMIDDMLVFQNDQVEKVDGVYKIKDSYYNGVRATRYLGE
                     YESEVGKVVDPSIVMETEYNGVLNTYFAPGSRYELH"
     gene            complement(336..1055)
                     /locus_tag="PK612_01315"
     CDS             complement(336..1055)
                     /locus_tag="PK612_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94786.1"
                     /translation="MKKQGLAFLLLGTFLLTGCNKPPKMEKVQIMYKYSNLTEVINIV
                     DDENQTALEQLELKITDKENFVLVSYADYTCSCWSVFRDHVLRNYINTKKIPIYVIET
                     DALGNDFKGLPIRKDLTNTPVIGIFAGGVCKYSIDYTSKSEIFIERDKFNEWMDARIK
                     DPLMTYISLEEVNTLLKGTEPFLLNWSYSICPDCVALDKQFMPGYIETLKKAPRCLIT
                     LSKVKQFVTLVIGLMLKTFMA"
     gene            1347..2873
                     /locus_tag="PK612_01320"
     CDS             1347..2873
                     /locus_tag="PK612_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94787.1"
                     /translation="MDKLTKHNVKKEDVIIELKDISKEYDGKTVVDDFNLYVKRGEFI
                     TFLGPSGCGKTTTLRMIAGFELPTTGTITLNGEDITKLPPYKRPVNTVFQRYALFPHL
                     DVYDNIAFGLKLKKVKDTKNGKEVMRKLTSKEIDEKVVRALKMVDLEQFEARDIATLS
                     GGQQQRVAIARAIVNEPEILLLDEPLGALDLKMRKEMQIELKDLHKRLGITFIYVTHD
                     QEEALTMSDTIVVMNDGLIQQIGTPLDIYNEPKNPFVADFIGESNIYNATMAGKLKVK
                     FLGATFECVDDLPTNEKVDVVIRPEDIKIVAAGKGNVDGKIISRVFKGVHYEYDVQVG
                     RKSEVIIQSTIEYPENEKVGLAVAPDDIHIMKKEVTTNVYADAWIDNKNRLMIGDNAF
                     EIDITQLLKGSTVDEEGYLVGPDAKKYDLDDVDVVATIDFKDIELDDVIEEDAPMGQI
                     ISIIWLGDHYQVIVRTNDEEDFIVDSEYLWNEGDLVSIIIKPEDIKLKLKGELVPYEN
                     "
     gene            2863..3666
                     /locus_tag="PK612_01325"
     CDS             2863..3666
                     /locus_tag="PK612_01325"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH94788.1"
                     /translation="MKIRFKRSYIGIPYVLFMVLFVLLPLFFVAFYAFTNSAGRFSFE
                     PMANFFTSSVKWRVLFVSFIFGILNTALCLVIGYPLAMILANRKYNTNAVLVMLFVMP
                     MWINFIIRSWAMRDVLVFLGIRGGEYPELATLLGLTYNYLPFTILPLYSTMLKMDKNQ
                     IEASYDLGAGPVTTFFKVIVPMTMPGIISAATMVFMPTMTSYVIADILGEGKVTLFGK
                     YIEIYFTQNIWNEGSFLALVMLALIGISVLVPKLLTRKEAKVKGVGIYD"
     gene            3659..>4231
                     /locus_tag="PK612_01330"
     CDS             3659..>4231
                     /locus_tag="PK612_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94789.1"
                     /translation="MIKKILAKVYVYLILFLLYLPILFLIIYSFTPAETTGVWEGFSF
                     ELYGRVFLNEKIMRALLNTLIIALSSATVATILGTLGAIGVFYMKKRPQRIMEGINEI
                     PVVNAEIVVAISLAILFRVFANFLEARGVATNSPNFVTLLIGHVVLSISFVYLNVKPK
                     LQQMDPNDYEAALDLGAKPMYAIRKVVLPAI"
BASE COUNT         1324 a          702 c          829 g         1376 t
ORIGIN      
        1 gtgaagttca tagcgcgatc ctggagcaaa ataagtgtta agcacaccgt tatattcagt
       61 ttccataacg attgaaggat caacaacttt tccgacttca ctttcatatt ctcctaaata
      121 gcgggtagct ctcacaccat tgtagtagga gtcttttatt ttataaacac catctacttt
      181 ttcaacttgg tcattttgga agacgagcat gtcgtcaatc atcggactaa tgtcattatt
      241 cgctaaataa gtagcgccat taccatctgg tcgaataact tgtaaagtag gaacatagcc
      301 tgtcgcgtaa cctgaaacag tattattttt atcacttaag ccataaatgt ctttaacatt
      361 aagccaatta ccagcgtcac gaattgtttt actttcgata atgtaataag gcatcttggc
      421 gcttttttaa gtgtttcaat gtagcccggc ataaactgtt tatcaagggc cacgcagtct
      481 ggacaaatac tatagctcca attaagtaaa aatggttccg tgccttttag tagtgtatta
      541 acttcttcaa gactaatata agtcatcagc gggtctttaa ttcttgcgtc catccattca
      601 ttaaatttat cgcgttcaat aaagatttca ctttttgatg tgtaatcaat actatattta
      661 caaacgccac ctgcaaaaat accaataacc ggagtgttcg ttaaatcttt gcgaatcggt
      721 agtcctttaa agtcgttacc taatgcatct gtttcaataa cataaattgg gattttcttc
      781 gtatttatgt agtttcttaa tacatggtca cggaacacac tccagcatga acatgtgtaa
      841 tcagcgtaac ttactaacac aaaattttct ttatcggtga tttttaattc aagctgttca
      901 agcgctgttt ggttttcatc atcaacaatg tttattactt ccgttaagtt tgaatattta
      961 tacatgattt ggactttttc catttttggt ggtttattgc aacctgttaa tagaaaagta
     1021 cctaataata agaatgctaa accctgtttt ttcataagtt tttacctcga tttcactcta
     1081 tatgtgagtt tattttatca agttttaata gtcattgcaa catgcagaaa aaagggtgaa
     1141 aaatttacaa aaaaagaggg tgaaaaatag acccataaat tagacgaaaa aagggggtaa
     1201 aaattcgaca attttttatt tgaaatcttt atcatataat gcaataaaat ctgctttttc
     1261 ggggtgccaa aaaaagtgta aaataattat gcttgtattt tattaaaatt tgccttatat
     1321 tttattgtgt tgaaaggggg gtacctatgg acaaattaac aaagcataac gtgaagaaag
     1381 aagacgttat cattgaatta aaagatatta gtaaagagta tgatggaaag acagtagtgg
     1441 atgattttaa cttgtatgtg aagcgtggtg aattcattac atttttagga ccatctggct
     1501 gtggtaaaac aacgacttta cgcatgattg ccggttttga attaccgaca acagggacga
     1561 ttacacttaa cggagaagat attaccaaac tcccaccata taaacgtcca gtaaatacgg
     1621 tatttcaacg ctacgcatta ttcccacatt tagatgtgta tgacaatatt gcttttggct
     1681 taaaacttaa aaaagtcaaa gacacaaaaa acgggaaaga agtaatgcgt aaacttactt
     1741 ctaaagaaat tgatgaaaaa gttgtgcgcg ctttaaaaat ggttgatctt gaacaatttg
     1801 aagcacgtga tatcgcgacg ctctcaggtg gtcaacaaca acgggtcgca attgcgcgcg
     1861 cgattgttaa tgaaccagaa attttattac ttgatgaacc gcttggcgca cttgacctga
     1921 agatgcgtaa agaaatgcaa attgaattaa aagatttaca taaacgctta ggtattactt
     1981 ttatttatgt tacgcacgac caagaagaag ctttaacaat gagtgataca atcgtcgtga
     2041 tgaatgacgg acttatccaa caaattggaa cgccgttaga tatatataac gaaccaaaga
     2101 acccgtttgt tgctgacttt atcggtgaat caaatattta taatgcgaca atggcgggca
     2161 agcttaaagt gaagttttta ggcgcgacat ttgagtgtgt tgatgactta ccgacaaatg
     2221 aaaaagtcga tgttgtgatt cgtcctgaag atattaaaat tgttgccgct ggtaagggta
     2281 atgttgacgg gaaaattatt agccgtgtct ttaaaggtgt gcactatgaa tatgatgtgc
     2341 aagtaggccg taaaagtgaa gttattattc aaagcactat tgaatatccc gaaaatgaaa
     2401 aggtcggctt agctgttgcc cctgatgata ttcatattat gaaaaaagaa gtgacaacaa
     2461 atgtgtacgc tgatgcatgg attgataata agaatcgctt aatgatcggt gataatgctt
     2521 ttgaaatcga tattactcag ctcttaaaag ggagcactgt tgatgaagaa ggttatttag
     2581 tgggacctga tgctaaaaaa tatgatttag atgatgttga tgtcgttgcc actattgact
     2641 ttaaagatat tgaacttgat gacgtcattg aagaagatgc gccaatggga caaataatca
     2701 gcattatttg gcttggtgac cactatcaag ttatcgttcg tactaatgac gaagaagact
     2761 ttatcgttga ttccgaatac ttatggaacg aaggtgactt agtaagtatt atcattaaac
     2821 ctgaagatat taagctaaaa ctaaaaggag aattagtgcc gtatgaaaat tagattcaaa
     2881 cggtcctaca ttgggattcc ctatgtgctc ttcatggtgc tcttcgtctt attaccgctt
     2941 ttctttgtag cattttacgc cttcactaat agtgcggggc gctttagttt tgagccaatg
     3001 gcgaacttct ttacaagtag tgtgaaatgg cgtgttttat tcgtttcctt tatttttggc
     3061 atattaaata cagcactatg cttagttatc ggctatccgt tagcgatgat tttagcgaat
     3121 cgaaaatata acactaacgc agtattagtg atgctctttg ttatgccaat gtggattaac
     3181 ttcattattc gctcatgggc gatgcgtgat gtacttgtgt tccttgggat tcgtggcggt
     3241 gagtatccag aacttgccac ccttttaggg ttaacttata actatttacc ttttaccatc
     3301 ttgccgcttt attcaacgat gctaaaaatg gataaaaatc aaattgaagc aagttatgac
     3361 ttaggcgctg gcccagtaac aactttcttt aaagtaattg taccaatgac gatgcccggg
     3421 attattagtg cagcgacaat ggtctttatg ccaacgatga caagttacgt tatcgcggat
     3481 attttaggag aaggtaaagt gacattattt gggaagtata ttgaaattta tttcacccaa
     3541 aacatttgga atgaaggctc attccttgcg cttgtaatgc ttgcgcttat cggtattagt
     3601 gtccttgttc ctaaactctt aacaagaaaa gaagcaaaag tgaagggggt cggtatttat
     3661 gattaagaag attctcgcaa aagtatatgt atacttaatt ttattccttt tgtacttacc
     3721 aattttattt ctcattattt atagctttac tcccgcggaa acaacaggag tatgggaagg
     3781 ctttagtttt gagctttatg gacgcgtctt tttaaatgaa aagattatgc gcgccttact
     3841 taatacactg attattgcgc ttagtagtgc aacggttgcg acaatcttag gcactcttgg
     3901 ggcaattggt gtcttttata tgaaaaagcg tccgcaaaga ataatggaag gaattaatga
     3961 aatacctgtt gttaacgcgg aaatcgttgt agcgattagt ttagctattt tattccgggt
     4021 gttcgctaat ttcttagaag cacgtggtgt agcgacaaat agtccaaact ttgtcacact
     4081 tttaataggg catgttgtgc ttagtatttc ttttgtgtat ctcaatgtta aacctaaact
     4141 ccaacaaatg gatccaaatg attatgaagc agcgcttgac ttaggtgcaa aaccgatgta
     4201 cgcgattcgt aaagtcgtgt tacccgcaat t
//
LOCUS       DAOSEX010000040         4208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_19052_length_4208_cov_3.594751, whole genome shotgun sequence.
ACCESSION   DAOSEX010000040 DAOSEX010000000
VERSION     DAOSEX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19052_length_4208_cov_3.594751"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK612_01335"
     CDS             <1..313
                     /locus_tag="PK612_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOH94790.1"
                     /translation="KKLYDEVSKHGLLITEYPNDTKPEPDYFKFRNRLVAALTKAVLI
                     IEAKYRSGTIITVGYALEKGSDVFCVPERAGRNSGCNRLIKDGAYLVESASDIIKLWP
                     N"
     gene            422..2377
                     /gene="topA"
                     /locus_tag="PK612_01340"
     CDS             422..2377
                     /gene="topA"
                     /locus_tag="PK612_01340"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOH94791.1"
                     /translation="MKLVIVESPSKIKTIERYLSDEYKVLASYGHIVDLSTRGRKNLG
                     INLETFEGDYVLDKSRQKVADTLKKAKNESSEVYLATDPDREGEAIAVHIAKLLNLDL
                     NTTKRLEFHEITRDGITNALAAPRLLNEDLFNSQEARRMLDRIVGFELSSLLNQKINV
                     KSAGRVQSAVLKLIVDKENEITAFVPEEYWTLKIDTKYDDALFSVNFNKSYAGDTKIV
                     NEAMKNDIVSKLAPKIVVTDVKSEIKHTNPKPPFTTSTLQQEASSKFKYSASRTSRII
                     QKLYEGIQINGIETGLVTYIRTDSTRLAPSFVANASQRIVKEYGAEYLGRTQARGKTL
                     QNVQDAHEAIRPTNLNFTPDSIKDSLTREEYNLYSLIYTRALASLMVGSKEQVTRYTF
                     NSNGTIFNATSTILIFPGFRKIYGKDEETVDDSALPQMHVGSELPIIDVITEQKFTEP
                     PARYSEGRLIKTMEELGIGRPSTYAPTIEILQKRKYVSSLKGTLKPTDIGFRQIDALS
                     EFFPSIISSDYTASMENELDKIANGEGDKVEFLSDFYSDFSQKIDDARKGMKKLPPEK
                     VGRDCPKCGSNLVYREGPYGRFIGCETFPKCKYTEFIVEYTGENCPKCNEPLTYRYDS
                     RNRKYIRCSNYKSCDYSATIKSTRRKK"
     gene            2423..3715
                     /gene="trmFO"
                     /locus_tag="PK612_01345"
     CDS             2423..3715
                     /gene="trmFO"
                     /locus_tag="PK612_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOH94792.1"
                     /translation="MEQVVIIGGGLAGVEAAFFLAEKGIKVKLYEMRPGVMTPAHHSA
                     KLGELVCSNSLKSKELTNACGLLKKEMEIYNSLMTSVAARVSVPGGNALCVDRELYSE
                     EVTRIITTHPLIKVVNEEVVELPSHVPVIIASGPLTSKGLENTISKLIDTAFLHFFDA
                     SAPIIEKDSINMDIAYYKSRYDQGDDAYINCPFTKEEYYRFYDELINAKLAPIHEMDT
                     KYFDACMPLEVIAKRGPKTLRFGPLKPRGLGKDEHHKPYAVLQLRQDNVIGSLYNLVG
                     FQTNLTYAEQRRVFKLIPGLENAEFVRYGLMHRNTFVNAPRVLNLDKSLKVNNDIYIA
                     GQLSGVEGYVESAASGLYVALNVYAKIKGVKLEYPCETMLGSLMHYLNNANPENFAPM
                     NANFGIVRGATKQNRMEIAEKSLKITQKYKEYIESLNE"
     gene            3708..4166
                     /locus_tag="PK612_01350"
     CDS             3708..4166
                     /locus_tag="PK612_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOH94793.1"
                     /translation="MNRPLQEFQDYLRYTRRFSENTVIAYISDIETFYLFLANQNMRD
                     DEVDKPLIRLYLKDQIDKYVSRRSIQRRLASLRHYYSFLQDKRYVKDNPFLTVQAPKA
                     TKALPRVLYEEEIRLILLANEKRTDYLRDRDQLIIELMFATGLRAVGFFI"
BASE COUNT         1417 a          726 c          814 g         1251 t
ORIGIN      
        1 taaaaaacta tatgatgaag ttagtaagca cggtctttta ataactgagt atcctaatga
       61 tacaaagcca gaaccagatt actttaaatt tcgcaataga cttgtagcgg cgctcacaaa
      121 agccgtttta atcatcgaag ctaaatatcg ctcaggcaca ataataacgg ttggttacgc
      181 cttagaaaaa gggagtgatg ttttctgtgt tcctgagcgc gctggccgta atagtggctg
      241 taatagactg ataaaagatg gtgcttatct tgtcgagagc gcaagtgaca ttataaaact
      301 ctggccaaac taagaaaata aaaaatgttt tatttattat aaataaagag aaaattcaca
      361 aagttttaca tttgacaagc gcaaaaaaac cctctatatt ttataaccga taggaggctt
      421 tgtgaaacta gtaattgttg aatccccaag caaaattaaa acgatcgaac gctacttaag
      481 tgacgagtac aaagtactcg ctagctatgg tcatatcgtc gacctttcaa cacgtggtcg
      541 caagaattta ggtattaatc ttgaaacttt tgaaggtgac tatgtattag ataagagtcg
      601 gcaaaaagtt gcagatacgc taaaaaaagc taaaaatgaa agtagtgaag tctaccttgc
      661 cactgaccct gatcgtgaag gtgaagcaat tgcggtgcac atcgctaaac ttttaaacct
      721 tgatttaaat acgactaagc gtcttgagtt tcatgagatt acgcgggacg ggattactaa
      781 tgccctcgct gcgccacgct tgcttaatga agaccttttt aatagccaag aagctagaag
      841 aatgcttgac cgtattgttg gctttgaact gtctagtctc ttaaatcaaa aaataaatgt
      901 taaaagcgct gggcgagttc aaagtgcggt tttaaaacta atcgttgata aggaaaacga
      961 aatcactgct tttgtccctg aagaatattg gacactaaaa attgacacta aatatgacga
     1021 tgctcttttt agtgtgaatt ttaataagag ttacgctggt gatactaaga ttgttaacga
     1081 agcgatgaaa aatgatatcg ttagtaaact agcgcctaaa attgttgtga ctgatgttaa
     1141 aagtgaaatt aaacacacta atcctaagcc gccttttacc acatcaacct tgcaacaaga
     1201 agcgagtagt aaatttaaat atagtgcttc ccgcacttca cggattatcc aaaaactcta
     1261 tgaaggtata caaattaacg ggattgaaac ggggttagta acttatattc gtactgactc
     1321 gacaagactc gcgccaagct ttgtcgctaa tgcatcacaa cgcatcgtta aagaatatgg
     1381 ggccgaatat cttggtcgta ctcaagcgcg tggtaaaaca ctacaaaatg tccaagatgc
     1441 tcacgaagcg attcgtccca cgaacttaaa ctttactcct gattcaatta aagattcatt
     1501 aacgagagaa gaatataatt tgtattcact tatttacaca cgagcacttg caagtttaat
     1561 ggtcggtagt aaagaacaag ttacgcgtta tacatttaat tcaaatggta ctattttcaa
     1621 tgcgacatca acaatcttaa tattcccggg ctttagaaaa atttatggga aagatgaaga
     1681 aacagtagat gactcagcgc ttccgcaaat gcatgttggt agtgaattac caattattga
     1741 tgttataact gaacaaaaat ttactgaacc acctgcgcgt tacagtgaag gccgcttaat
     1801 taaaacgatg gaagaactcg gtattggtcg accatcaact tatgcaccaa cgattgaaat
     1861 attacaaaaa cgtaaatatg tatctagcct taaaggtacg cttaagccaa cggatattgg
     1921 ctttagacaa attgatgctc ttagcgaatt tttcccaagt attattagta gtgattacac
     1981 tgcttcaatg gaaaatgaat tagataaaat agcaaatggc gaaggcgata aagttgaatt
     2041 tcttagtgat ttctacagtg actttagtca aaaaattgat gatgcgcgca aaggaatgaa
     2101 gaaactaccg ccagaaaagg tcggtcgcga ttgtccaaag tgtggctcaa acttagtgta
     2161 ccgcgaagga ccatatggtc gctttattgg ctgcgaaact ttcccgaaat gtaaatacac
     2221 cgaattcatt gttgaataca ctggtgaaaa ttgtccaaaa tgtaatgagc ctttaactta
     2281 tcgttatgat tcacgtaatc gtaaatatat tcgttgctca aattataaaa gttgtgatta
     2341 ttccgcaact attaaaagta ctagacgtaa aaaataactt atttttgtag ggaaattgcc
     2401 gttttcttgc tataatctca ttgtggaaca agtagtaatt attggtggtg gattagcagg
     2461 agtagaagcg gctttttttc ttgcggaaaa agggattaaa gttaagttat atgaaatgcg
     2521 tcctggtgtc atgacgcctg ctcatcatag cgcgaagctt ggtgaacttg tgtgttctaa
     2581 ttcattaaaa agtaaagaac ttacaaatgc atgcggttta cttaaaaaag aaatggaaat
     2641 atataattca ctaatgacaa gtgttgctgc gcgtgttagt gttccaggcg gtaatgctct
     2701 ttgcgttgat cgtgaacttt atagcgaaga agtaacaagg attattacta ctcatccgct
     2761 tattaaagta gtgaacgaag aagtagtcga acttccttct catgttcctg ttattattgc
     2821 cagcggacct ttaactagta aaggcttaga gaacacgatt agtaaactta tcgatacagc
     2881 gtttctacat ttttttgacg ctagtgcacc aattattgaa aaagattcaa ttaatatgga
     2941 tattgcttat tataaatcgc gatatgatca aggtgacgat gcttatatta attgtccatt
     3001 tacaaaagaa gaatattatc gattttatga tgaattaatt aacgctaaac tagcgccaat
     3061 tcatgaaatg gatacgaaat actttgatgc ctgtatgcca ttagaagtaa tagcgaagag
     3121 aggacctaaa accttacgct ttggaccact taaaccacgc ggacttggta aagatgagca
     3181 tcataaacct tatgctgtcc ttcaacttcg gcaagataat gtaattggct cgctttataa
     3241 cttagtcggc tttcaaacaa acttaactta tgctgaacag cgccgtgtat ttaaacttat
     3301 acccggttta gaaaacgctg agtttgttcg ttacggatta atgcaccgca atacatttgt
     3361 taatgcaccg cgtgtcctaa atttagataa gtcacttaaa gttaacaatg atatttatat
     3421 tgctggtcaa cttagtggcg ttgaaggtta tgttgaaagt gccgctagcg ggctttatgt
     3481 tgcccttaat gtttacgcta aaataaaagg tgtaaaatta gagtatccgt gtgagacaat
     3541 gcttggtagt ttaatgcatt acttaaataa cgccaatcca gaaaactttg ctccaatgaa
     3601 cgctaatttt ggaatagttc gaggtgctac taaacaaaac cgcatggaaa ttgccgagaa
     3661 atcattaaaa ataacgcaaa aatataagga gtacattgag tcattaaatg aatagacctt
     3721 tacaagagtt tcaagattac ttacgttaca ctagacgctt ttcagaaaac acagtcattg
     3781 cttatataag tgacattgaa actttttatt tatttctcgc taatcagaat atgcgtgacg
     3841 acgaggttga taaaccatta atccggttat atttaaaaga tcaaattgat aaatatgtat
     3901 cgcggcgcag catccagcgc cggcttgcca gcttacgcca ctactattca tttttacaag
     3961 ataagcgcta tgtgaaggat aatccctttt taacagtaca agcgccaaaa gcaacaaaag
     4021 cattgccacg cgtactttat gaagaagaga ttcgcttaat cttactcgct aatgaaaagc
     4081 gcactgatta tttacgtgac cgtgaccaat taattattga attaatgttt gcaacagggt
     4141 tgcgcgctgt ggggtttttt atttaaaact tcaattatta ttagtcaata tgtgttccat
     4201 aattaaaa
//
LOCUS       DAOSEX010000041         4179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_19238_length_4179_cov_3.484481, whole genome shotgun sequence.
ACCESSION   DAOSEX010000041 DAOSEX010000000
VERSION     DAOSEX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19238_length_4179_cov_3.484481"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PK612_01355"
     CDS             <1..467
                     /locus_tag="PK612_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOH94794.1"
                     /translation="DNVNPTIISFAPEFMMYKLHTVGRLDKDTTGALLLTNNGKLTHQ
                     LISPKKNVEKVYLATTDLPIKGELVAHFARGIKIDEDFTTLPATLEIVESNVGRVTVV
                     EGKFHQIKRMFQAFGLNVVKLHREQFAGLRVDDLEIGEYRKLEHDEINSISI"
     gene            504..1112
                     /locus_tag="PK612_01360"
     CDS             504..1112
                     /locus_tag="PK612_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH94795.1"
                     /translation="MVRRTTSRTKSTTKTRRDDILEAGIRLFARVPFSELTIAAVARE
                     ANCGHSLVYHYFTNINRLYDECVNHVVARYKPFIEHFKSLTIPAELALAGSIALIVEM
                     LKREKLAPYCLSLLLYTYKQAPRNKAILTVREDWSKLFLNFISEGQKNERIISTLSAN
                     EILNVLKSILRGLISDQIVSGPSPANILTASEIYLPIMQGIN"
     gene            1113..2936
                     /locus_tag="PK612_01365"
     CDS             1113..2936
                     /locus_tag="PK612_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94796.1"
                     /translation="MFRLYKLVKRYWWQLAIIVVLVATQTLLQLQLPDYITTTQNIVN
                     NIGETGITRAEINEMLKQSGIMLLISLGIFALAAGQGSLNSKFVATLGKEIRAEVFKK
                     VENFSLSDVNEFGTASLLTRLINDSRAVQEFAFMVNRVFIMSPIFLIIGSVKLLTLDP
                     MYFYVVIVIIPLIIIVLIITFAVASPLFTQIQERVDYVTLLLREGLTGVRVIRAFNQE
                     ERENRFFDEGNRKMTDIIIKVSNVMQIISPIINILFNLVFFGIYLIGFVLFDKMLLGV
                     IPFNMNPFSQTITAMMYAQHIMMSFMMLSFTFIMFPRVKVSAKRINAVLNVKPNIVDP
                     EAPLDKELIDTGIVKFENVTFTFSDLEHPTLKNINFTAEPGTTTAIVGSTGSGKSSII
                     NLLPRFFDASVGKVTFDGYDVRDFKLNDLRDKIGFVPQTAVLFKGTIRDNLRFGNENA
                     TDEEMWEALRVAQAAGFVSKLELGLDAPVAQGGKNFSGGQRQRLAIARALVRKPKIYV
                     FDDSFSALDFKTDIRLRSALKDYAKDATIFVVAQRVNSILDAHQILVLDNGEVAGIGK
                     HADLLNSCKVYQEIVTSQLDPEEIKKSRDMFKDFTTEGGKA"
     gene            2936..>4179
                     /locus_tag="PK612_01370"
     CDS             2936..>4179
                     /locus_tag="PK612_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH94797.1"
                     /translation="MARHGRGRKMEKARNSKLAWRGLLREFKPYRWRIIIIAVLLTIA
                     NVANALIPLLLRNILGAFSPENVRYGRYFLDVTNLALGLDWTKVFIDFGLMIGVIVAA
                     ALLGTIAQLIAIKISGAYAYNLRNKIRAKIDRLPLSYFDKHPHGELLSIGTNDVDAIA
                     QTLNNTLITVVSSITLFVAILIGMLLVNWRLALVALGMLPFVVIITLVITKNSQNQFI
                     TFQKKTGELEGVVEENFSGLQVIQLFNQQDKQLETFTNLNDSMAKSNFWSQWLSSFIF
                     PSIRFINNLGFVGVLLVAGVINDVISLAPFIFFLNNFMQPFLNLGQISANIQTTLAGA
                     ERVFNLLNAAEQEKDPEDALTTNDHVVGEFCFKDVDFSYNPDVELIKNMNLHVKPGDT
                     IAIVGPTGAGKTTLVNLIMRFY"
BASE COUNT         1294 a          689 c          774 g         1422 t
ORIGIN      
        1 aagataatgt taaccctaca attatttctt ttgcgccaga atttatgatg tataaattac
       61 atactgttgg tcgtttagat aaggatacga caggcgcctt actattaaca aacaacggta
      121 aacttacaca ccaacttata agtccaaaaa agaatgttga aaaggtttat cttgctacca
      181 ccgatttacc tattaaaggt gaattagtcg cgcattttgc gcgtggaatt aaaattgatg
      241 aagattttac gacacttcct gcaactttag aaatagtgga atctaatgta ggtagagtta
      301 ctgttgtgga aggtaaattt catcaaataa aacggatgtt tcaagcgttt gggttaaatg
      361 ttgtaaaact tcatcgcgaa caatttgcag gactgcgtgt tgatgattta gaaattggtg
      421 aatatcgaaa attagaacat gacgaaatta attcaatatc tatttaggtt catctttgtt
      481 ataatttttg caatggtata actatggtaa gacgaacaac aagtagaact aaaagtacca
      541 caaaaactcg acgcgacgat attctcgaag caggaatacg actttttgct cgtgtccctt
      601 tttctgaatt aacaattgcc gctgttgcgc gcgaagctaa ttgtggtcat agtcttgttt
      661 atcattattt cacaaatatt aatcgcttat atgatgaatg tgtcaatcat gttgttgctc
      721 gttataagcc ttttattgaa cactttaaat cattaacaat tcccgctgaa ctagcgctcg
      781 cgggttcaat tgctttgatt gtagaaatgt taaaaaggga gaaactagct ccttattgtt
      841 taagtctgtt gctatatact tacaaacaag caccacgtaa taaagcaata ttaacagtgc
      901 gggaagattg gtcaaaactt tttttaaatt tcattagtga aggccaaaaa aacgagcgta
      961 taatcagtac attaagcgct aatgagatat taaatgtttt aaaatcaata ttacgcggtt
     1021 taattagtga ccaaattgtt tctggtccgt cgcccgctaa tatcttaaca gcgtctgaaa
     1081 tttacttacc aattatgcag gggataaact aaatgtttag actttataaa ttagttaaac
     1141 gttattggtg gcaattagcg attattgttg tcctcgtcgc tactcagaca ttgcttcaac
     1201 ttcaattacc tgattatatt acaacaactc aaaacattgt taataatatt ggtgaaactg
     1261 ggataactcg cgctgaaata aatgaaatgt taaaacaaag cggtattatg cttttaattt
     1321 cccttggtat ttttgcttta gcagcaggcc aaggctcatt aaactctaaa tttgttgcta
     1381 cccttggtaa agaaattcgc gcggaagtgt tcaaaaaagt tgaaaacttt tcattaagtg
     1441 atgttaatga atttggaacc gctagtcttt taactaggct aattaacgat tcacgcgctg
     1501 tgcaagaatt tgcttttatg gtaaatcgtg tttttattat gtcaccaatc tttttaatta
     1561 ttggttctgt taaattacta acattagatc caatgtattt ctatgtcgtt atcgttatta
     1621 ttcctttgat tataattgta ttgattatta cttttgccgt agcaagcccg cttttcaccc
     1681 aaattcaaga acgagtggat tatgtgacgc tattattacg cgaaggacta actggtgttc
     1741 gtgtcattcg tgcctttaat caagaagaac gtgaaaaccg cttctttgac gaaggcaatc
     1801 gtaaaatgac tgacattatt attaaagtta gtaatgttat gcaaattata agtccgatta
     1861 ttaatatctt atttaactta gtgttttttg gaatatatct tattggtttt gtcttattcg
     1921 ataaaatgct ccttggtgtt attcctttta atatgaatcc ttttagtcaa acaataacag
     1981 cgatgatgta cgctcaacat attatgatga gtttcatgat gcttagcttt acctttatta
     2041 tgttcccgcg cgttaaagtt agcgcaaagc gaattaacgc tgtactaaat gttaaaccaa
     2101 atattgttga tccggaggca ccgcttgata aagaattaat tgatactggt attgttaagt
     2161 ttgaaaatgt aacttttact ttcagcgatc ttgaacaccc aacattaaag aatattaatt
     2221 ttacagcaga gccaggaact acaacagcaa ttgttggttc tactggttct ggcaagagtt
     2281 caatcattaa tttattacca cgattttttg atgctagtgt tggtaaagta acttttgacg
     2341 gctatgatgt gcgtgacttt aaattaaatg atttacgcga caaaataggc ttcgttccac
     2401 aaactgcagt gttatttaaa ggtacaattc gtgataattt acgcttcggt aatgaaaacg
     2461 ctactgatga agaaatgtgg gaagctttac gcgtggcgca agctgcgggt tttgtttcta
     2521 aactcgaact tggacttgat gcaccagttg cacaaggtgg caaaaatttc agtggtggtc
     2581 aaagacaacg cctagcgata gcgcgtgcac ttgttagaaa acctaaaatt tatgtgtttg
     2641 atgattcttt tagtgcacta gactttaaga ctgatattag actaagaagc gcattaaaag
     2701 attacgcaaa agacgcaact atttttgttg tagcacagcg tgttaattct attcttgatg
     2761 cccatcaaat cttagtcctt gataatggcg aggttgctgg tattggcaaa catgctgact
     2821 tacttaattc gtgtaaggta tatcaagaaa tcgttacatc acaactagat cctgaagaaa
     2881 ttaaaaagtc gcgtgatatg tttaaagact ttacaactga agggggtaaa gcataatggc
     2941 acgccacgga cgcggacgca aaatggaaaa agcgcgcaat tcaaaattag cttggcgtgg
     3001 tcttttgcgc gaatttaaac cttatcgttg gcggataata attattgctg tgcttcttac
     3061 tatcgcaaat gtagctaatg cgcttatccc gctattattg cgtaatattc tgggtgcttt
     3121 ctcgcctgaa aatgttcgtt atggtcgtta ttttttagat gttactaatc tagcgttagg
     3181 gttagattgg acaaaggtct tcattgattt tggattaatg attggtgtta ttgttgccgc
     3241 tgcattactt ggtactatcg cacaactgat tgctatcaaa atttctggag cgtacgctta
     3301 taatcttcgt aataaaatac gcgcgaaaat tgatcgttta ccactttctt attttgataa
     3361 acatccgcat ggtgaattac tttcaattgg cacaaatgat gttgatgcta ttgcccaaac
     3421 attaaataac acgcttatca ctgttgtttc atcaatcact ctttttgttg caattttaat
     3481 tggcatgtta cttgtaaatt ggcggcttgc cttagtagca cttggcatgc ttccgtttgt
     3541 agtaattatt acgcttgtta ttacgaaaaa ttcacaaaat cagtttatta ctttccaaaa
     3601 gaaaacaggt gaattagaag gggtagtgga agaaaacttt tccggactcc aagtcattca
     3661 actatttaat caacaagata aacaactaga aacttttaca aatcttaatg attctatggc
     3721 taaatctaat ttctggtcac aatggctgag ttcatttatt ttcccatcaa ttcgctttat
     3781 taataatctt ggcttcgttg gtgttttact tgtagcgggt gttataaatg atgttattag
     3841 tctcgcgcca tttatttttt tcttaaataa ctttatgcaa ccattcctta acttagggca
     3901 aatatctgca aatattcaaa caacacttgc tggtgcagaa agagtgttta atttacttaa
     3961 tgccgctgaa caagaaaaag atccagagga tgcattaact acaaacgatc atgttgtggg
     4021 ggaattctgc ttcaaagatg ttgattttag ttataaccct gatgttgaac ttattaaaaa
     4081 tatgaattta catgttaaac caggggatac aatcgctatt gttggtccaa ctggcgcggg
     4141 caaaacaaca ctagttaatt taattatgcg attctatga
//
LOCUS       DAOSEX010000042         4173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_19280_length_4173_cov_3.169500, whole genome shotgun sequence.
ACCESSION   DAOSEX010000042 DAOSEX010000000
VERSION     DAOSEX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19280_length_4173_cov_3.169500"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1262
                     /locus_tag="PK612_01375"
     CDS             120..1262
                     /locus_tag="PK612_01375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOH94798.1"
                     /translation="MENFFVIVSAFFYLSIMTASFIFLNRQDDQNPRRFRVVVLLYVF
                     TILGIILDTLYRLDYGGLLNLPSYAFAGVITAAYACLPVVWGLWYLYISHTSCLKEIP
                     RAYNIVVAATLLLNLALSLISLIPGTNIYFTIVDGVYARGKLFFVQTIISLLFLALPI
                     CVYTRFFKTFWKSERFTFLLIITTPILALVGHLLNNFIPFMVMNNVFNYMILAMNVQY
                     IHASTDFLTGLANRQSLSNHLHSRMTGTEKMSSFSIIMFDLDNFKMINDTLGHDVGDD
                     ALRQFARFLRHHCDNVDFVARFGGDEFIIVLDSMSPAELKKRVAVIREQFSEYEGNKV
                     GNFKLVFSAGYFIHNKDLKISTGELIRIIDERMFRDKRKRSNGKTN"
     gene            complement(1325..1966)
                     /locus_tag="PK612_01380"
     CDS             complement(1325..1966)
                     /locus_tag="PK612_01380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOH94799.1"
                     /translation="MSTAQINPTRMELQRLRDKLATTTRGHKLLKDKQDELIHEFIPL
                     LPEYRALREKVESALSATLTRYQFACVKMSKEAINDHINDSVTKHELSAKFDRVMGVV
                     IPNLETKASHTEVNYDLLSTTSAFDDLITAIQDLFALLIELANLENTIKILVAEIEKS
                     KRRVNAIENIVIKEIEEQIRVIRMKLTDLERSNTIRMMKSKEIIMEKKFNEGK"
     gene            complement(1970..3352)
                     /locus_tag="PK612_01385"
     CDS             complement(1970..3352)
                     /locus_tag="PK612_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOH94800.1"
                     /translation="MAIKAFKTIKEVVGPLMVVENVSGVKFDELVRIIQSNGEERLGK
                     VLEIDNDKAVVQLFESSQGLKIDDAKAIFTGEGVTFGVSPHILGRKFDGLGRPIDGKG
                     HIIPFKRIDINGVPLNPVARDYPNEFIQTGISAIDGLNTLVRGQKLPIFSSSGLTHNR
                     LAAQIAKQATVLGRDEKFAVVFAAIGITFEEANFFSEAFEESDALDRTVMFFNLANDP
                     AIERIATPRMAITAAEYLAYELGMHVLVILTDITNYAEALREISAARKEVPGRRGYPG
                     YMYTDLASLYERAGRVKGLKGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSRPL
                     SMKGLTPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFSAYARGKDAKELSLILGD
                     SALSEIDQKYAAFADAFERDYVSQGFDTNRSIKETLDLGWKLLGMLPRQELRRVDPKL
                     VDKYYKESKE"
     gene            complement(3357..>4173)
                     /locus_tag="PK612_01390"
     CDS             complement(3357..>4173)
                     /locus_tag="PK612_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004221417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOH94801.1"
                     /translation="EYFRDMGYRVAIMADSTSRWAEALREMSSRLEEMPGEEGYPAYL
                     ASRLSDFYERAGVVVTLGKKKRLGSITAIGAVSPPGGDTSEPVSQATLRIVKVFWGLS
                     ASLAYKRHFPAIDWLRSYSLYDDDIVKHHSASFPYWKDYVALLKELLQDEAALLEIVR
                     LVGIDSLSQKDRVKLHAAQMIREDYLHQNAFHAVDTYTSIEKQYGMLDLIVTWFKEAN
                     LALDEGVEYKQMENLAVNERISHIKYVEEKDFDKQVKALKKDVIAAFNNLKEE"
BASE COUNT         1173 a          860 c          726 g         1414 t
ORIGIN      
        1 aaaaaatgat tctttcagtt ttttatcttg tcaatttaac cttttcttct attatttaag
       61 tttaaaactt tgcaaaattt attatatata gatataatac aacacacagg actttatcta
      121 tggaaaattt ctttgttatt gttagtgcat tcttctacct gtcaatcatg acagcatctt
      181 ttatattttt aaatcggcaa gatgatcaaa acccacgtcg ctttcgggtt gttgtcttac
      241 tttatgtgtt tacaatacta ggtataatcc ttgatacact ttatcgttta gattacggtg
      301 gattacttaa tcttccttca tacgcctttg ccggagttat taccgctgca tatgcttgct
      361 taccagtcgt gtggggatta tggtacttat atattagtca cacttcatgt ttaaaagaga
      421 taccgcgcgc ttataacatt gttgttgctg cgaccttact tttaaattta gcgctttcac
      481 ttatatcttt aattcctggc actaatattt actttactat tgttgatggc gtatatgcta
      541 gaggtaaatt attctttgtc caaacaatta tttccctttt attcttagcg ctccccattt
      601 gtgtttacac gcgtttcttt aaaacatttt ggaaaagtga gcgctttact ttcttactta
      661 ttataactac tccgatatta gcacttgttg gtcacttatt aaataatttt attccgttta
      721 tggttatgaa taatgtattt aactatatga ttctggcaat gaatgttcaa tatattcatg
      781 cttctactga tttcttgact ggtcttgcta atcgtcagag cttaagtaac caccttcata
      841 gtcgcatgac tggtacggaa aaaatgagca gtttttccat tattatgttt gaccttgata
      901 attttaaaat gattaatgat acattgggtc acgatgtcgg ggacgacgct cttcgtcaat
      961 ttgcgcggtt cttacgccat cattgtgaca atgttgactt tgtcgcacgc tttggcggtg
     1021 acgaatttat tatcgttctt gatagtatgt cgcccgctga acttaaaaaa cgtgttgcgg
     1081 tcattcgcga acaatttagt gaatatgaag gtaataaagt cggtaatttt aaactagtct
     1141 tcagcgcggg atactttatc cataacaaag atttaaaaat tagcactggt gaacttattc
     1201 gcattatcga tgagcggatg tttagagata agcgtaaacg cagtaatggt aaaactaatt
     1261 aaaaaaatat acgcacacat agcgcgtacg tatattaata attaagtgtg gcaatcacac
     1321 ttttttattt accttcgtta aatttctttt ccatgatgat ttctttactc ttcatcatcc
     1381 gaattgtatt gctgcgttct aagtctgtta gtttcatgcg tatcacacga atttgttctt
     1441 caatttcttt aatgacaata ttttcaatcg cattaacacg gcgttttgat ttctcaattt
     1501 ccgccactaa gattttaatg gtattttcta agttagcaag ttcgatgagt aacgcaaata
     1561 aatcttgaat cgctgtgatt aaatcatcaa aggcactagt tgtcgatagt aaatcataat
     1621 taacttcagt gtgactagct tttgtttcta agttaggaat gactacaccc attacgcgat
     1681 caaactttgc acttaattca tgtttcgtta cactgtcatt aatatggtca ttaattgctt
     1741 ctttactcat tttgacacac gcaaattgat agcgagttaa agttgcactt aatgcacttt
     1801 caaccttttc ccgcagagcg cggtactcag gtagcagcgg aataaactca tgaattaatt
     1861 catcttgttt gtcttttaaa agcttgtgac cacgcgttgt ggtcgctagt ttatcccgta
     1921 accgttgtaa ctccatccgg gttggattaa tttgcgcagt gctcattaat tactctttac
     1981 tttccttata atatttatca acgagttttg gatcaacacg ccgtaattct tgccgtggta
     2041 acattcctaa aagtttccat ccaagatcaa gtgtttcttt aatactacgg ttagtgtcaa
     2101 agccttgtga aacataatct ctttcgaaag catcggcaaa agccgcgtat ttttggtcaa
     2161 tttcagaaag tgcactatca cctaaaataa gtgataattc ttttgcatct ttaccgcgcg
     2221 catacgcact gaagagctga ttcattgtat cagcgtgatc ttcacgcgtt ttacctttac
     2281 caattccttt atctttaagc cgtgaaagtg aaggtaaaac atcaatcggt ggcgttaagc
     2341 ctttcattga aaggggacgg gacaaaataa tttgtccttc ggtaatataa cccgttaagt
     2401 ctggaattgg gtgcgtttta tcatcttcag gcatcgttaa aatcggaatt tgggtaatgg
     2461 aacctttaag tccctttacg cgacccgcgc gttcatagag cgaggctaag tctgtgtaca
     2521 tatagccagg ataccctctt ctaccaggca cttctttacg cgcagcactg atttcgcgca
     2581 gtgcttcagc gtagttagta atatccgtta aaataactaa gacatgcatc ccaagttcat
     2641 aagcaagata ttctgccgcg gtaatcgcca tacgtggtgt tgcaatccgc tcaatcgctg
     2701 ggtcatttgc taaattaaag aacattacag tccggtcaag cgcatcactt tcttcaaatg
     2761 cttcactaaa gaagtttgct tcttcaaaag taatgccaat tgcggcaaat acaaccgcaa
     2821 atttttcgtc gcgtcctaac actgtcgctt gcttagcaat ttgcgcagcg agcctgttat
     2881 gtgttaaacc tgaactagag aaaattggta acttttgtcc gcgaacaaga gtatttaacc
     2941 catcgatggc actgatgccc gtttgaataa attcgtttgg ataatcgcgc gctactgggt
     3001 taagcggtac accattaata tcaatgcgtt taaaaggaat aatgtgccct ttgccatcaa
     3061 ttggccgacc aagaccatca aactttcttc ctaaaatgtg tggtgatacc ccaaaagtaa
     3121 caccttcacc agtaaagata gctttcgcgt catcaatttt taatccttga ctactttcaa
     3181 aaagttggac aacggcttta tcattatcaa tttctaaaac tttaccaagc cgctcttcac
     3241 cgttactttg aataatacgc acaagttcat caaatttaac accgctgaca ttttcgacaa
     3301 ccatcagggg gccaacaact tctttaattg ttttaaatgc tttaattgcc atatatttat
     3361 tcctccttca agttgttaaa tgccgcaata acatcttttt taagcgcttt aacttgttta
     3421 tcaaaatctt tttcttcgac atattttata tgacttatgc gttcattaac cgcaaggttt
     3481 tccatttgct tgtattcaac gccttcgtca agtgcaaggt ttgcttcttt aaaccaagta
     3541 acaattaaat caagcatccc gtattgcttt tcaatggaag tataagtatc taccgcgtgg
     3601 aaagcgtttt ggtgtaaata gtcttctcta atcatctgcg cagcgtggag tttgactcga
     3661 tctttttgac taagtgaatc aatacccaca agacgaacaa tttctagaag cgctgcttcg
     3721 tcttgtaata attcttttaa aagagcgaca taatctttcc aatacgggaa agaggcacta
     3781 tgatgcttaa caatatcgtc atcatacaaa gaataactcc gtaaccagtc gatggccggg
     3841 aaatgccgct tatacgctag actagcgctt aacccccaga acactttaac aatccgtaaa
     3901 gttgcttggg aaaccggttc actagtgtca ccgcctggcg gcgaaactgc gccaattgcg
     3961 gtaatcgaac ctaaacgttt ctttttacct aaagtcacaa caacaccagc tctttcatag
     4021 aagtcactta agcgacttgc taagtaagcg ggatagcctt cttcgcctgg catttcttca
     4081 aggcgtgaac tcatttctcg cagtgcttct gcccacctag aagtagagtc ggccataatc
     4141 gccacacggt aacccatatc acggaaatat tcc
//
LOCUS       DAOSEX010000043         4117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_19673_length_4117_cov_3.308469, whole genome shotgun sequence.
ACCESSION   DAOSEX010000043 DAOSEX010000000
VERSION     DAOSEX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19673_length_4117_cov_3.308469"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PK612_01395"
     CDS             <1..397
                     /locus_tag="PK612_01395"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOH94802.1"
                     /translation="LNPLRNKLQELFVLDYEKNKENSTVEFSSQVEEVYAFIQNAFGR
                     SLSAVELQTINDWFNYGYNITMIKEALDQTMKRKRYNIRAIDKALLKLSTAVDYALEG
                     KSAQSDSYRKNIKDTLEEVNKKAQDDESE"
     gene            381..1016
                     /gene="nth"
                     /locus_tag="PK612_01400"
     CDS             381..1016
                     /gene="nth"
                     /locus_tag="PK612_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOH94803.1"
                     /translation="MTKANKERILAFLDKEYSDAKGELEYTKDYELLIAVVLSAQSTD
                     VAVNNVTRKLFAKYPTFESLLNVKEAQLAAYFKTLGLYRNKAKHVYNLIHLLNEHHNG
                     VVLSAKEELLKLPGVGVKTANVVRAELFNIPEIAVDTHVSRIARRLGFAKFSDDVTII
                     EEKLRKVLPKERYIKTHHQMIHFGRYFCKAQSPLCKKCPLVDICKEPNKKL"
     gene            complement(1011..1601)
                     /gene="recU"
                     /locus_tag="PK612_01405"
     CDS             complement(1011..1601)
                     /gene="recU"
                     /locus_tag="PK612_01405"
                     /inference="COORDINATES: protein motif:HMM:NF002581.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HOH94804.1"
                     /translation="MINYPNGSKPKKQTTKVPSKRKNITKSAANRGMALEKLISAAND
                     YYLMHDIALFTKRPTPINVVKIDYAKGAKITNAYFEKQSTTDFNGVYKNRYFDFEAKS
                     TRLKTSFPLNNIYEHQITHLENVIRHGGIAFFIVEFTAFDEYYVLSAADVIDFYRNGD
                     RKSIPYASFKEKGVLVRLGLNPVLDYLPAVIKLFNL"
     gene            complement(1601..2326)
                     /locus_tag="PK612_01410"
     CDS             complement(1601..2326)
                     /locus_tag="PK612_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOH94805.1"
                     /translation="MKKLAKATRGRKQLIFLDLEGTQFSHELIAIGAIKATIKTDGTI
                     KKLYPGFKHYVLATQNVGNYVEKLTGITDDLLAREGIPFDKILLMLKKYVGTNLSKTK
                     FVTFGAHDIRILRQSLAHIPEADHTFIKTIAKNHIDLSAYINEYIKDEKNNTLSLVNL
                     CKLFGVEPVEPAHDPLNDALMLAYLYSELFKKTDILIERYGEVLLNMNNIPRPIKRML
                     NKIKAGESVNIDDLQEYLRDEIV"
     gene            2418..2768
                     /locus_tag="PK612_01415"
     CDS             2418..2768
                     /locus_tag="PK612_01415"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOH94806.1"
                     /translation="MAEKRLLDADKILKKKFKAKSGGYDALEVDEFFDLVRNDYESML
                     EIEKELELLRLKNETQQAKIVNLEAQYIQYKKKVEELERLISKGGTAMENLRKIDKYE
                     RQLWKMGIDPSKLK"
     gene            2773..3106
                     /gene="rnpB"
                     /locus_tag="PK612_01420"
     ncRNA           2773..3106
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK612_01420"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            3131..3769
                     /gene="pgsA"
                     /locus_tag="PK612_01425"
     CDS             3131..3769
                     /gene="pgsA"
                     /locus_tag="PK612_01425"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOH94807.1"
                     /translation="MNLPNKLTVARMILVILLIAFLLIPPTWLGNEPIILFSNVYLRY
                     FIAAIVFIVAAITDYFDGYLARKFNLITNFGKFMDPIADKMLVNSMLIILTVPHQAFV
                     GQISVPVIFVVLMIARDTVVDGFRLVTANQNKVLAANIFGKAKTVLQMIAIVMFLFND
                     WPFSHQMQSDSFPIVSMIVIGAATFVSLLSGFIYIFQNISVLKETHDDTTAH"
     gene            3747..>4117
                     /locus_tag="PK612_01430"
     CDS             3747..>4117
                     /locus_tag="PK612_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HOH94808.1"
                     /translation="MTTQLLINELIDKNLTIGSVESFTGGLFAATLVNYPGVSKTYKG
                     TIVAYSNDVKERLLNIKKEDLKKFSAVSSVVALDLARHGQKALGVDFCFGFTGDAGPT
                     ASGQSGEVGDVYIALAFANDE"
BASE COUNT         1378 a          651 c          781 g         1307 t
ORIGIN      
        1 acttaatccg ttacgaaata aattacaaga acttttcgtg ttagattatg aaaagaataa
       61 agaaaattcg acagtagaat ttagttcaca agttgaagaa gtttatgcct ttattcaaaa
      121 tgcttttggc cgttcacttt cagcagtaga actgcaaacg attaatgatt ggtttaatta
      181 tggttataac atcacaatga ttaaagaagc acttgaccaa acaatgaaac gtaaacgcta
      241 taacattcgc gctattgata aggcgctact taaattatcg actgctgtgg attatgcgct
      301 tgaaggtaaa agtgcccaaa gtgattcata tcgaaaaaac atcaaggaca cacttgaaga
      361 agtgaataaa aaagcccaag atgacgaaag cgaataaaga aagaatactc gcctttcttg
      421 ataaagaata tagtgacgct aaaggtgagc tagaatacac aaaagattat gaactgctta
      481 ttgctgttgt tttaagcgca caatcaactg atgtagcggt taataatgtt acgcgaaaat
      541 tatttgcaaa gtatccaact tttgaaagct tattaaatgt aaaagaagca caattggccg
      601 cgtattttaa aacacttggc ttatatcgca acaaagcaaa acatgtgtat aatttaattc
      661 atttacttaa tgaacatcat aacggagttg ttcttagtgc taaagaagag cttttaaaat
      721 tacctggtgt tggagttaaa accgcgaatg ttgtacgcgc tgaactcttt aacattcccg
      781 aaattgccgt ggacactcat gtttcacgaa tagcgcgtcg ccttggtttt gctaagttta
      841 gtgatgatgt aactatcatt gaagagaagt tacgtaaagt tttacctaaa gaaagatata
      901 taaaaacgca tcaccaaatg attcattttg gtcggtattt ttgtaaagca caaagtccgc
      961 tatgtaaaaa atgtccgctt gtggacattt gtaaagaacc aaataaaaag ttataaatta
     1021 aataatttta tgactgcagg caaatagtca agtacagggt taagaccaag gcgcactaat
     1081 acgccttttt ctttaaatga tgcataaggg attgatttac ggtcgccatt gcgataaaaa
     1141 tcaataacat cagcagcact aagtacataa tattcatcaa aagctgtaaa ttcaacaata
     1201 aagaaagcaa tcccaccatg gcgaataaca ttttctaagt gagtaatttg atgttcataa
     1261 atattattta aaggaaaact tgtttttaat cttgtacttt tagcttcaaa gtcgaaatat
     1321 cggtttttat aaaccccgtt aaagtctgtt gttgattgct tttcaaagta ggcgtttgta
     1381 atttttgccc ctttagcgta atcaattttt acgacattaa ttggtgttgg gcgtttagta
     1441 aaaagggcaa tgtcgtgcat caaataatag tcattagcag cactgataag tttttcaagc
     1501 gccatgccac gattagcagc gctcttcgta atattttttc gttttgaagg tactttcgtt
     1561 gtttgttttt taggtttaga accgtttgga taattaatca ttacacaatt tcatcgcgta
     1621 aatattcttg taaatcatca atatttacac tttctcctgc ctttatttta ttgagcattc
     1681 tttttattgg acgcgggata ttattcatat tgagtagtac ctcaccatac cgttcaataa
     1741 gaatatcagt ctttttaaag agctcactat ataaataggc gagcatcagg gcatcattta
     1801 acgggtcatg agcaggttca actggctcaa cgccgaaaag tttacataaa ttcaccaaag
     1861 ataatgtatt atttttttca tcttttatat attcattaat gtatgcactt aaatcaatat
     1921 gatttttcgc aatcgtttta ataaaagtat gatcagcttc aggaatatgg gcaagtgatt
     1981 ggcgtaagat acgaatgtcg tgtgccccaa atgtcacaaa tttagttttt gaaagattag
     2041 tgccaacata ttttttaagc atcagtagaa tcttatcaaa aggaatgcct tctcttgcta
     2101 ataaatcatc cgtaatgcca gttaattttt caacataatt accaacattt tgtgttgcta
     2161 gcacataatg tttaaagcca ggataaagtt ttttaatcgt tccgtccgtt ttaattgtgg
     2221 ctttaattgc cccaattgct attagttcat gactaaattg tgtaccttct aaatcaagga
     2281 agataagttg cttgcggcca cgtgttgctt tcgctaattt tttcatacta taaaattata
     2341 acatagtaat aataaagcgg tctcactttg ttgcgtcaaa acaaaattac atatataata
     2401 tgtatgcgag gttaacgatg gcagaaaaga gattactaga cgcagacaaa attttaaaaa
     2461 agaagttcaa agcgaaaagc ggtggctatg acgctttaga agttgacgaa ttttttgatt
     2521 tagtgcgtaa tgactatgaa tctatgctcg aaattgaaaa agaattagaa ttactgcggc
     2581 tgaaaaatga aactcaacaa gctaagattg ttaatcttga agcgcaatac attcaatata
     2641 aaaagaaagt tgaagagcta gaacgcttaa ttagtaaagg cggaaccgcg atggaaaact
     2701 tacgtaaaat tgataagtat gagcgtcaat tatggaaaat gggcattgac ccaagcaaac
     2761 tcaaataaaa ttcgatctgg ataattgctg gtacataagt attagaggaa agtccatgct
     2821 cgcacttgct gcgatgcaag tagtgattgc gctagtggaa aaaataacac taggtataga
     2881 ggagtctata acggcgaaga aagacctaag tcgaaagata tggttaatct tctgaaagtg
     2941 ccacagtgac gaagttaagg ggaaactctt aagtggaacg ttggtaaacc ccgcaagcga
     3001 gaaacctaaa tttggtaagg gaaacggcaa tagagaaatg aatttagcgc cgtatgtgtt
     3061 cacacataga taaataatta tccgcgacag aacatggctt acaggatcga aactcaccta
     3121 aaggagcatt atgaatttac cgaataaatt aacagtagcg agaatgatac ttgtcatcct
     3181 tttaattgct tttttactta ttccgcccac ttggttaggt aatgaaccaa ttattctttt
     3241 ttctaatgtt tatctgcgtt attttattgc cgcaattgtt tttatagtag cggcaatcac
     3301 tgattacttc gatggatact tagcgagaaa atttaattta attacaaatt ttgggaagtt
     3361 tatggaccca attgccgaca aaatgctggt gaattctatg ttaatcatcc ttacagttcc
     3421 gcatcaagcg ttcgtcgggc aaataagtgt acctgttatt tttgttgtac tcatgattgc
     3481 gcgtgataca gtagtggacg ggtttagact tgttactgca aatcaaaata aagtactagc
     3541 ggcgaatatt tttggtaagg ctaaaacagt tttacaaatg attgcaattg tgatgttttt
     3601 atttaatgat tggccctttt cacaccaaat gcaaagtgat tcgtttccga ttgttagcat
     3661 gatagtaatt ggcgctgcta catttgtgtc attattatca ggctttattt atatattcca
     3721 aaatatcagt gtacttaagg agacgcatga cgacacaact gctcattaat gaattaattg
     3781 ataaaaattt aacaattggt agtgttgagt cttttactgg tggtttattt gcggcaacac
     3841 tcgtgaatta tccaggtgtt agtaaaacat ataaagggac gattgtggct tatagtaacg
     3901 atgtcaaaga gcgtttactt aacattaaaa aagaagatct aaaaaaattt agtgccgttt
     3961 caagtgttgt agcgcttgat ttagcgcgtc atggccaaaa agcattagga gttgattttt
     4021 gttttggctt tacgggtgac gcaggtccaa cagcatcagg ccaaagcgga gaagttggtg
     4081 atgtttatat tgccctagcg tttgctaacg acgaaat
//
LOCUS       DAOSEX010000044         4110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_19722_length_4110_cov_3.183724, whole genome shotgun sequence.
ACCESSION   DAOSEX010000044 DAOSEX010000000
VERSION     DAOSEX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19722_length_4110_cov_3.183724"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..763
                     /gene="rplD"
                     /locus_tag="PK612_01435"
     CDS             119..763
                     /gene="rplD"
                     /locus_tag="PK612_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOH94809.1"
                     /translation="MSKAVKKTITYPVVNTKGEKVGTMRLNGEIFGIEPHQDVVHQAV
                     VTYLANRRQATAKTKTRAEVRGGGKKPWRQKGTGRARAGSRRSPIWVGGGVTFGPTGE
                     QNYKLSMNKKAHALALRSALSIKAAEKAFIIIDDTIEIEGKTKAAAELLKNVGATGKV
                     LLINDDNKSLLLATRNLPNVQSVGQDNVAVYDLLNADQIVVEKATLVAYVGGLE"
     gene            763..1107
                     /gene="rplW"
                     /locus_tag="PK612_01440"
     CDS             763..1107
                     /gene="rplW"
                     /locus_tag="PK612_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOH94810.1"
                     /translation="MSKRPRKKAEQVVQVKKPTVEQFNLLLRPVITEKSMALMQDQNK
                     VTMEVHKTANKESVKDAFEAVFGVTVTDVKIINTNSKKTRRGGRYYGTIPGVKKAVIT
                     VAEGQAIDLFKE"
     gene            1128..1961
                     /gene="rplB"
                     /locus_tag="PK612_01445"
     CDS             1128..1961
                     /gene="rplB"
                     /locus_tag="PK612_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOH94811.1"
                     /translation="MAIRKYKPTTPSRRNMTNLTFEQITKTKPEKSLTVELKRTGGRN
                     NMGKITTRHIGGGHKRRYRIIDFRREKDGVEGVVAAIEYDPNRNANIALIHYLDGEKR
                     YILHPQGLAVGDRIVSGEAVDIKVGNAMPLAAIPEGSVVHNVELTPGKGGQIARAAGT
                     SVQILGREGKYAILRLQSGEVRKVLQACRATVGQVGNEDYNLINLGKAGKSRWKGIRP
                     TVRGSVMNPVDHPHGGGEGKSPIGKDAPRTPWGKRALGVKTRKQNKKSTQLIIRRRKQ
                     K"
     gene            1975..2274
                     /gene="rpsS"
                     /locus_tag="PK612_01450"
     CDS             1975..2274
                     /gene="rpsS"
                     /locus_tag="PK612_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOH94812.1"
                     /translation="MARSLKKGPFADKSLLKKVDELNKANKKQIIKTWSRRSTIFPSF
                     VGHTFAVYNGREHITVYVTEDMVGHKLGEFSPTRSYRGHTGHTAEDKAAAAQRKR"
     gene            2282..2689
                     /gene="rplV"
                     /locus_tag="PK612_01455"
     CDS             2282..2689
                     /gene="rplV"
                     /locus_tag="PK612_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOH94813.1"
                     /translation="MARIKKPKTTEKVETKEVKEKPVVTEARAISRSVGVTPRKMRLV
                     ADLVRGKKVSDAIALLLNINKAASVPLRKTIESAAANAINNHGFKEEGLYISELQIND
                     SIKLKRYRPRAKGGAAGVTKRTSTIIVKVKESV"
     gene            2703..3368
                     /gene="rpsC"
                     /locus_tag="PK612_01460"
     CDS             2703..3368
                     /gene="rpsC"
                     /locus_tag="PK612_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008790813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOH94814.1"
                     /translation="MGQKVNPNGLRYGIYRNWNSRWFAKNKDFATFLKEDMDIREFLT
                     KELKEALLSHIEIDRVNTFESGYSVIVSVHVARVGVVIGQQGERVKELTAKLAKIVKS
                     GEVKINVVDIKNPDLNASLVAQDIARQLEARGSFRIVQKRTIQRVRRAGAKGIKTLVS
                     GRLNGAEIARSEGYKDGIIPLHTLRADIDYALAEAHTTYGRLGVKVWICRGEYDLSKG
                     RGE"
     gene            3371..3793
                     /gene="rplP"
                     /locus_tag="PK612_01465"
     CDS             3371..3793
                     /gene="rplP"
                     /locus_tag="PK612_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOH94815.1"
                     /translation="MLQPKRTKYRRPQRLSYEGLTKGGANIEFGEFGLQALEGAYITN
                     RQIESARIVLASYTRRVGKIWIRIFPHLAKTKKPLEVRMGSGKGAPDSWVAVVKKNRI
                     LFEIGGVKEEIAREALRLAAYKLPIKTRVVSKADNKED"
     gene            3796..3993
                     /gene="rpmC"
                     /locus_tag="PK612_01470"
     CDS             3796..3993
                     /gene="rpmC"
                     /locus_tag="PK612_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOH94816.1"
                     /translation="MKIKEIRALSTTELKEKQYLLIEELFHLRRKQAVGQLENGKEIT
                     RVRKDIARVYTILRERELGIN"
BASE COUNT         1428 a          859 c          939 g          884 t
ORIGIN      
        1 caggtcctaa atacggaata ttgaaagtta gaagtgcggt taaaaagcaa ttacgcaaac
       61 gcgaagttaa agacttagtc gatcgcaccg ctaaagaata agaatggagg ttatagttat
      121 gtcaaaagca gtaaagaaaa ctattactta tccagttgtt aacactaaag gggaaaaagt
      181 tggcacaatg cgacttaatg gcgaaatctt cggtattgag ccgcatcaag atgtcgtcca
      241 tcaagcagtc gtgacttacc ttgctaaccg gcgtcaagct accgcgaaaa caaaaacacg
      301 cgcagaagta cgcggtggtg gtaagaaacc atggcggcaa aaaggaacag gccgtgcccg
      361 cgctggtagt cggcgttcac ccatctgggt tggcggtgga gtcacctttg gtccaaccgg
      421 agaacaaaac tacaaattat caatgaataa gaaagcgcac gcgctcgcat taagaagtgc
      481 gctctcaatc aaagctgcgg aaaaagcatt catcattatc gatgacacca tcgaaattga
      541 aggcaaaaca aaagccgctg cggaattact taaaaatgtt ggggccactg gtaaagtctt
      601 attaattaat gacgacaata agagcttact acttgccacc cgcaacttac caaacgtcca
      661 aagcgttggt caagacaatg tcgccgttta tgacttatta aacgctgacc aaatcgtcgt
      721 cgaaaaagca accttagttg cgtatgtagg aggacttgaa taatgagtaa acgtccacgc
      781 aaaaaagcag aacaagtcgt acaagttaaa aaaccaacgg ttgaacaatt caacctcttg
      841 cttagacccg tcattactga aaagtcaatg gcgctcatgc aagaccaaaa caaagtaacg
      901 atggaagtgc ataaaaccgc aaataaagaa agtgttaaag atgcatttga agcggtcttc
      961 ggtgtaacag ttaccgatgt aaagatcatt aacacaaatt caaaaaaaac acggagaggt
     1021 ggtcggtact acggcacaat cccaggcgtc aaaaaagctg tgattaccgt tgctgaagga
     1081 caagcaattg acctcttcaa agaataaaga cgggaaggag aaacattatg gcaattagaa
     1141 aatataaacc gacaactccc tcacgtcgga acatgacgaa ccttacgttc gaacaaatta
     1201 cgaaaacaaa accagaaaaa agcttaaccg tcgaacttaa acgcactggt ggtcgtaata
     1261 acatgggaaa aattactacc cgtcacatcg gtggtggtca taaacgcaga taccgcatta
     1321 ttgacttccg tcgggagaaa gatggtgtag aaggcgttgt cgcggcaatc gaatacgatc
     1381 caaaccgcaa cgcaaatatc gccttaatcc actacttaga cggtgaaaag cgttacatct
     1441 tacatccaca aggactcgcg gtcggcgatc gcatcgtctc gggtgaagca gtcgacatca
     1501 aagtcggtaa cgcaatgccg ctcgcagcaa tcccagaagg tagtgtcgta cataacgttg
     1561 aattaacccc tgggaaaggc ggacaaatcg cccgcgctgc gggaacaagt gtccaaatct
     1621 taggacgcga aggaaaatat gcaatccttc gactccaaag tggtgaagta cggaaagtat
     1681 tacaagcttg tcgcgctaca gtcggacaag ttggtaatga agactacaac ttaatcaact
     1741 taggaaaagc tggtaagagt cgctggaaag gcattcgccc aacagtccgt ggctctgtga
     1801 tgaacccagt tgaccaccca cacggtggtg gtgaagggaa gtcaccaatt ggtaaagacg
     1861 caccaagaac accatggggc aaacgcgcct taggtgtgaa aacgcgcaaa caaaataaga
     1921 aatcgacgca attaattatc agacgtcgca aacaaaaata aggagtgagg aaacatggct
     1981 agaagtttaa aaaaaggtcc attcgctgat aagtctctct taaaaaaggt tgacgaatta
     2041 aataaagcga ataaaaaaca aattattaaa acatggtcgc gcagatcaac catcttccca
     2101 tccttcgtcg gtcatacatt cgcagtgtat aacggacgtg aacacattac agtgtatgtg
     2161 acggaagata tggtcggcca taaattaggc gaattctccc caacccgctc ataccgcgga
     2221 catacggggc acacagctga agacaaagcc gcggccgcac aacggaaacg gtaggtgaat
     2281 tatggcaaga attaaaaaac caaaaacaac agaaaaagtt gaaacaaaag aagttaaaga
     2341 aaaacctgtg gttaccgaag cacgcgcaat cagtcgcagc gtcggagtca ccccacggaa
     2401 aatgcgctta gtcgctgact tagtccgcgg gaaaaaggtg agtgatgcaa tcgcgctctt
     2461 actaaatatt aataaagccg cgagtgtacc gcttcgcaaa acaatcgaaa gtgccgcagc
     2521 gaacgccatc aacaaccatg gcttcaaaga agaagggctc tacattagtg aattacaaat
     2581 taatgattcg attaaattaa aaagatatcg cccgcgcgct aaaggcggag cggctggtgt
     2641 cactaaaaga acaagcacaa ttattgtgaa agtaaaagaa agcgtgtaaa ggaggaaaag
     2701 taatgggaca aaaagttaat ccaaacggtc taagatacgg tatctatcgt aactggaata
     2761 gtcgttggtt tgccaaaaac aaagacttcg ctactttcct caaagaagac atggacattc
     2821 gtgaattctt aacgaaagaa ttaaaagaag cattactctc acatattgaa attgatcgcg
     2881 tgaacacatt cgaaagtggc tatagtgtca tcgtcagtgt ccacgtcgcc cgtgtcggtg
     2941 tcgtgattgg ccaacaaggc gaacgcgtaa aagaattaac cgctaaatta gccaaaattg
     3001 tgaagtcggg tgaagttaaa attaatgtcg ttgatattaa aaacccagac ttaaatgcaa
     3061 gtttagtggc gcaagacatc gcgcgtcaac tcgaagcacg tggttcattt agaatcgtgc
     3121 aaaagcggac gatccaacgc gtccgtcgcg ctggcgctaa aggaattaaa actttagtca
     3181 gcggtcgctt aaatggtgcg gaaatcgccc gtagtgaagg ttataaagac ggaatcatcc
     3241 cgcttcatac cttacgcgct gatattgatt acgcacttgc agaagcacac acaacttacg
     3301 gccgtcttgg tgtaaaagtg tggatttgtc gtggtgaata cgacttaagt aaagggagag
     3361 gagaataatt atgttacaac caaaacgtac taaatatcgt cgcccccaac gcttgagtta
     3421 tgaaggctta accaaaggtg gcgcaaatat cgaatttggc gaatttggac ttcaagcact
     3481 tgaaggtgcc tacatcacta accgtcaaat cgaaagtgca cggattgtgc ttgctagtta
     3541 tacacgccgt gttggtaaga tctggattcg tatcttccca cacttagcga aaactaaaaa
     3601 accacttgaa gtgcggatgg gttccggtaa aggggctcca gactcatggg tcgccgttgt
     3661 caaaaagaat cgtattctct ttgaaatcgg tggcgttaaa gaagaaatcg ctcgtgaagc
     3721 actccgcctc gcagcgtaca aattaccaat taagacacgc gtagtgtcta aagcagacaa
     3781 taaggaggat taatgatgaa gattaaagaa attcgtgcgt taagtacaac cgaattaaaa
     3841 gaaaaacaat atcttttaat cgaagaatta tttcacctcc gtcggaagca agccgttggt
     3901 caattagaaa acggtaaaga aattactcgc gtccgaaaag atatcgcgcg tgtatatact
     3961 atattacgcg aacgcgaact cgggattaat taaggagcag tgataatatg gaacaaacag
     4021 taagaaaagc acgcgctacg tttgacggga ttgtcattag tgataaaaat gataaaacaa
     4081 tcaccgtctt agtacaaaca tacaaacggc
//
LOCUS       DAOSEX010000045         4035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_20246_length_4035_cov_3.364573, whole genome shotgun sequence.
ACCESSION   DAOSEX010000045 DAOSEX010000000
VERSION     DAOSEX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20246_length_4035_cov_3.364573"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PK612_01475"
     CDS             <1..370
                     /locus_tag="PK612_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94817.1"
                     /translation="HVYNKDVLPLNSLKWLFDVWGPQWHETGAGGLASSFAPYAEYKF
                     VISGTIDNNEDTDEGWSFEIYIPFSELGVTNVDSDYIKILHAVGSHEQNNMLPQEYLD
                     LHPDVNWDYPEDYPRISKPV"
     gene            384..1829
                     /locus_tag="PK612_01480"
     CDS             384..1829
                     /locus_tag="PK612_01480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH94818.1"
                     /translation="MFKKKWMPLLLSIPLLLTGCKGKTSDSVTIFIYGQAHERSIYSK
                     IIEKFTEETGIKVNAELVNTDGYAETLTASLGTKNAPDVFYSNPASIGRYAMNGVILD
                     LKPYLEIEAEVEDGLDYKNFLDGVVDYYRYDVTTKKRGEGEAVWAIPKDVSSYPLGYN
                     RNIINLSVELGHWATHTKKGAWASREGGMPLPWENDPRTDKQIVYTFEEFTNACRATK
                     FIHNEIEYFGTALIDTWAMHSWIWAAGGNWLSDDGKTVTVNTPEFIAGFTEFIDLMDN
                     RGISQTREQMGVKPHYDRWMAGEVAFFNVGVWDVAAFESVPKELLDYGLMPTPRKDNA
                     SPEWYTYIGTLGYAVSSRTRVAQEAVKLALYMSMSTESYNRMTKYQTIQLPNNKLMVD
                     EYLSDETIKPENKGIFLDVIQKGHGKLHPTAMAYNSLWYDAFIAELSRVWKVTDDDPS
                     GEMSPAQYAAYVQPIMQAQLNIALADEAAGV"
     gene            1899..2807
                     /locus_tag="PK612_01485"
     CDS             1899..2807
                     /locus_tag="PK612_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH94819.1"
                     /translation="MNTNIVEKPKKRKVKHVQENIIGYLFILPVFIGIAFFIVYPFFY
                     SLVSTFKNWNGLVPLSVAPTVGFEMYRRVLSDSLFWQAFGNTLITLIGIPIGMILSIA
                     IAMLLNRGLKFTGIFRVIFYIPVVSSITAIIILWRAMMNTQGPINDFLALFGVKAINF
                     LGEPGWARFSLIMMLVWKGLGTSILLYLAGLQGISDTYKEAAKIDGANDWQIFWHVTF
                     PLLGPTHFFIVVTGIIGGMQLYVEPELLLGGGPSRATTTLLIYLFERFGAHRISEASV
                     VAWVLGVFVFIITAIQFYTNYRREKA"
     gene            2804..3658
                     /locus_tag="PK612_01490"
     CDS             2804..3658
                     /locus_tag="PK612_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013523906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH94820.1"
                     /translation="MKKSNIKANRSIKRAKLFSDISTYVILVLGSVLMLAPLIWMLSA
                     SLKVNQFSVYGPLFHEWNFFNYIEVFKTIPVWKGLLNSVIVAVPSIIVGTFVSGLAAY
                     AFAKIKFKFCNIIFILMLAVMMIPFPVIMIPQYYIFSDLDWIGTLLPLIIPKLFGNIM
                     MIFFLRQFLTHIPDALVESAKIDGANHFQIFWIIILPLLKPALAAQTVLWFIGIWNDY
                     LAPNFFSAASPTLPVVIGGLVSDFPIQEPVHLNMAASIISMIPVLAIFAIFQKQIINS
                     FVLSGMKE"
     gene            3673..>4035
                     /locus_tag="PK612_01495"
     CDS             3673..>4035
                     /locus_tag="PK612_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HOH94821.1"
                     /translation="MKTKLFIDKNLTIGKVSNLLFGSFIEHLGRAVYTGIYEPGHPKA
                     DKDGFRQDVIDLIKELKVPMVRYPGGNFLSGYNWEDGIGPKENRPVRLDYAWQTIEDN
                     TFGTDEFMRWCKKAEIEPM"
BASE COUNT         1201 a          680 c          828 g         1326 t
ORIGIN      
        1 acatgtttat aataaggacg ttttaccact taattcactt aaatggctct ttgatgtatg
       61 gggaccacaa tggcacgaaa ctggcgctgg gggtctagca tcatcttttg cgccatatgc
      121 tgaatataaa tttgtgatta gcggcacaat tgataataac gaagatactg atgaaggatg
      181 gagttttgaa atttacattc cgttctctga actcggtgtt actaatgttg actccgatta
      241 tataaaaatt cttcacgctg ttggcagtca tgaacaaaat aatatgcttc cacaagaata
      301 cttagacttg catccagatg taaactggga ctaccctgaa gattatccac gcatttcaaa
      361 acccgtttaa aggagggaat aatatgttta aaaagaaatg gatgccatta ttactatcga
      421 ttccactgct tttaactggt tgtaaaggta aaacaagtga tagtgttact atctttattt
      481 atggccaagc tcatgagcga agtatttatt caaagattat tgaaaagttt actgaagaga
      541 cgggaattaa agtcaatgct gaactcgtta acacggatgg gtatgctgag acattaactg
      601 catctttagg aacaaaaaac gcgcctgatg tcttttattc aaacccagcg tcaattggtc
      661 gttatgcgat gaatggcgtt attctcgatc ttaaacctta tttagaaata gaagcagaag
      721 tggaggatgg gcttgattat aaaaatttct tagatggagt cgttgattac tatcgttatg
      781 atgtgacaac caaaaagcgt ggggaagggg aagccgtatg ggcaatccca aaagatgttt
      841 cctcttatcc tttaggctac aatcgtaata ttattaatct gtcggttgag cttggtcact
      901 gggcaacaca tacgaaaaaa ggagcatggg caagtcgtga aggcgggatg cctttgccgt
      961 gggaaaatga tccgcgtact gacaaacaaa tcgtatatac atttgaagaa tttacaaatg
     1021 catgtcgcgc tacaaagttt attcataacg aaattgaata ttttggtact gcgctaattg
     1081 atacatgggc aatgcattca tggatttggg cagcaggtgg taactggtta tcagatgatg
     1141 gtaagacggt tactgtcaat actccagaat ttatcgctgg ttttactgaa ttcattgatt
     1201 taatggataa ccgcggtatt tctcaaacac gcgaacaaat gggggttaaa ccacactatg
     1261 atcgctggat ggcgggtgaa gtagccttct ttaatgttgg tgtatgggat gttgccgcat
     1321 ttgaaagtgt accaaaagaa ttattagatt atggtttaat gccaacacca cgaaaagata
     1381 atgctagtcc agagtggtat acatatattg gtacattagg ttacgctgtt tcttcacgaa
     1441 caagagttgc gcaagaagca gttaaactag cgctttacat gagtatgagc accgaatcgt
     1501 ataatcgtat gactaaatat caaacaattc aattaccaaa taacaagcta atggttgatg
     1561 agtatcttag tgatgaaaca attaaacctg aaaacaaagg aattttcctt gatgttattc
     1621 aaaaaggaca cgggaaactc catccaacag ccatggctta taactcatta tggtatgacg
     1681 cttttatcgc tgaattatca cgcgtttgga aagttactga tgatgaccca agtggtgaaa
     1741 tgtcgccagc acagtacgct gcttatgtgc agccgataat gcaggctcaa cttaatattg
     1801 cactagccga tgaagcggct ggtgtgtaat taatttatat cattgctcgg acatcgatcc
     1861 tctcgatgtt cgggcataga ttagatgagt aatatattat gaacacaaat attgtagaaa
     1921 aaccaaaaaa aagaaaagtt aaacatgttc aagaaaacat aataggatat ttatttattt
     1981 tgccagtttt cattgggatt gctttcttta tcgtatatcc ttttttctat tcattggttt
     2041 caacttttaa aaactggaat ggacttgttc cccttagtgt tgcgccgaca gttggatttg
     2101 agatgtatcg gcgagtgtta agtgattcat tattttggca agcctttggt aataccctta
     2161 ttacccttat tgggattcca attgggatga ttctttcaat tgcaattgca atgttgttaa
     2221 accgcggact taaatttacc ggcattttcc gtgtaatttt ctatattccg gttgtttcct
     2281 caattactgc aattattata ttatggcgag caatgatgaa tacccaagga ccaattaatg
     2341 attttttagc tctctttggt gtaaaagcaa taaacttcct tggtgaacca ggatgggccc
     2401 gtttctcgct tattatgatg cttgtatgga agggtctagg aacatcaatc ttactttatt
     2461 tagcgggttt acaaggcatt tctgatactt ataaagaagc cgctaaaatt gatggtgcta
     2521 atgactggca aatattttgg catgttactt tccctttatt aggcccgacg catttcttta
     2581 ttgtagtgac cggtattata gggggaatgc agctttatgt tgagccagaa ttacttttag
     2641 gtggcgggcc aagtagagca accacgactc tcttaattta cttatttgag cgttttggtg
     2701 cccaccgtat tagcgaagct tcagttgtcg cgtgggtgct aggtgtgttt gtctttatta
     2761 ttaccgctat tcagttttat acgaattatc ggagggaaaa agcatgaaaa agtcgaatat
     2821 caaagcaaat cgttcaatta agcgtgccaa actcttttcc gatattagca cttatgtaat
     2881 tttggtactt ggatcagtgt taatgttagc gccacttatt tggatgctat cagcgtcatt
     2941 aaaagttaac caattttctg tttatggtcc tttatttcat gaatggaact tctttaacta
     3001 tatcgaagtg tttaaaacaa ttccagtgtg gaagggtctt ttaaatagtg ttatagtcgc
     3061 cgttccgtca attatagtag gtacatttgt ctctgggtta gcggcttatg ctttcgcaaa
     3121 aattaaattt aaattctgta atataatctt tattttaatg cttgcggtta tgatgattcc
     3181 ttttcctgtt attatgattc cgcagtatta catattctca gatttagatt ggattggaac
     3241 tttattacca cttattattc caaagttgtt cggtaatatt atgatgattt tcttcttacg
     3301 gcaattcctt acacatattc ccgatgcttt ggttgagagt gctaaaattg atggcgctaa
     3361 tcattttcaa attttctgga taattatttt accgctttta aaacccgctt tggcggcgca
     3421 aacagtgtta tggtttattg gcatttggaa tgattactta gcccccaatt tcttctcagc
     3481 ggcttcacca actttacccg ttgttattgg tggattagta agtgacttcc caatccaaga
     3541 accagttcat ttaaatatgg cagcgtcaat tatttcaatg attccagttc tcgcaatttt
     3601 tgctattttc caaaaacaaa ttattaattc attcgttctc tcgggaatga aagaataaga
     3661 aggagtaaca atatgaaaac aaaattattt atcgacaaaa acctaacgat tggtaaagtt
     3721 agtaatcttc tttttgggag ttttattgaa catttaggaa gagcagtgta tacgggtatt
     3781 tatgaaccag gtcaccctaa agctgataaa gatggctttc gtcaagatgt tatcgattta
     3841 attaaagagc ttaaagtccc aatggtgcgt tatcctggtg gtaactttct ttctggatat
     3901 aattgggaag acgggattgg tccgaaagaa aatcgtccgg ttcgcctaga ttacgcttgg
     3961 caaactattg aagataacac attcggcact gatgaattta tgcgctggtg taaaaaggcg
     4021 gaaattgaac caatg
//
LOCUS       DAOSEX010000046         4010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_20433_length_4010_cov_2.732238, whole genome shotgun sequence.
ACCESSION   DAOSEX010000046 DAOSEX010000000
VERSION     DAOSEX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20433_length_4010_cov_2.732238"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..1009
                     /locus_tag="PK612_01500"
     CDS             476..1009
                     /locus_tag="PK612_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOH94822.1"
                     /translation="MAKNGKNRLDDEDYRVPTKERYLYIDLEGGGGRIWVNKEDYQNY
                     SRIINREWKARIDASRCLIIAERGMYKRCREDCAQCPLARTGNHISYDAIIENTGNEL
                     PDSFDMRVEHEYDELRDKLWEEVSKLDKLDQKILTLYNEEFTEREIAKEVGLSQKAVN
                     LRIRKCVELLKEKLKNY"
     gene            1219..1536
                     /locus_tag="PK612_01505"
     CDS             1219..1536
                     /locus_tag="PK612_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94823.1"
                     /translation="MSNFEQVDKLLVKLIDEINAWRKELKELYAPETKGTETVPEQAV
                     ELVPITLVEVRTRLAELSRDGKTNQVRALIEKFGASKLSEIDPKHYPAIMDAAVEIDH
                     GKR"
     gene            1523..2653
                     /locus_tag="PK612_01510"
     CDS             1523..2653
                     /locus_tag="PK612_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HOH94824.1"
                     /translation="MARDNGHALIGGSSLSRAMECPPSVRLTEHYDDESSIYAKEGTS
                     AHLLLEKKVKRRYGIPFEESNEELEYYSEEMEETTDIALNFISEVYEKLVEEGKHPFI
                     ASEVLVDFSDVVPEGSGSSDVVIVYDGGIYVIDYKHGRGVEVSCENNPQLMIYAYGSM
                     RMFEALYDTVDINMVIIQPRLNNISEWSCTKDELVDWAVNEVKPKTALAYEGKGEFKC
                     GPWCKFCKARRDCRHRAEEMLKIEGYRVKDKALLTIEEIAEILGKIDDFISWGNDIKE
                     FALTEALSGKQIPGFKLVEGRSNRRYTSEEDVVKAVEEAGLDPFEKKLLSITELTKRL
                     GKKKFTELVDKYVYKPAGKPTLVEETDKRPVINLARIDFMEE"
     gene            2660..3214
                     /locus_tag="PK612_01515"
     CDS             2660..3214
                     /locus_tag="PK612_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HOH94825.1"
                     /translation="MSKTNPCKVITSVVRFSYLNANEPRATTEGAVPKYSVSLIIPKT
                     DTDLVKRIHDAIEAAYKEGEFKLRGASKSVPPLTAIKNPLRDGDLERPDDPAYKGSYF
                     VNANSTSKPEVVDAALNPIIETSEIYSGIYGRASITFYAFNSNGSKGIACGLNHLQKI
                     RDGEPLGSKATAESDFGDNEGFLD"
     gene            3269..>4010
                     /locus_tag="PK612_01520"
     CDS             3269..>4010
                     /locus_tag="PK612_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94826.1"
                     /translation="MISKLSIDLETYSATSLNAGVYKYTEDPAFEILLFAYSINDGPV
                     EVVDLAMGDKVPQIVLDALINPNITKWAFNAQFERICLSRFLGLPDNTYLSPQGWKCS
                     MTWAASLGLPFSLKDVGKVLNIDKKKIEEGKDLIRYFCLPCKPIKQNGHRLRNYYYHD
                     KEKWEQFKFYNKRDVEAEMAIQERLANHPMSESLWEEYWMCELINDRGVLVDTMLSRN
                     AIKINGICRGKYLKTLQNITELENPNSVA"
BASE COUNT         1383 a          704 c          888 g         1035 t
ORIGIN      
        1 agacagaaac agatattgtt tagtggtagg aagttttgcg tcacataata tgcgaagaag
       61 agtacctaaa caaagttttt tcaaataaaa aatacctttc tagaatttat ttatttctat
      121 attaaatttt atcattttgt atacaatttc acaatttttg tatttttcac aacttttaaa
      181 tttagattta atagaaacga atttataaat ttgccgttca tttgccgact aatgccgtct
      241 gcgtgctgtt ttaataaata tttacaaatc taaaatgaag gcaagaagga caaattagat
      301 ctttctaaaa tcgatgattg ccgaaagcta gcaacacaat atagcgccat catcgcataa
      361 cagttcattt gtgagaactg tctgcaaacc agtggagtaa tccagtttgg aagcaggcat
      421 aacctagttg aactcttttc atgctgcctt actccgctag aacggagaac cgcacatggc
      481 aaaaaatggc aaaaacagac tagacgacga agattaccgc gtaccgacga aggaaagata
      541 cctctatatc gatcttgaag gtggtggtgg gcgcatttgg gttaataaag aagactatca
      601 aaactatagc aggattatta atagagaatg gaaagcaaga atagacgcct cacggtgttt
      661 aattattgct gaacgaggaa tgtataagcg ttgccgagaa gattgcgctc aatgcccttt
      721 agcgcggact ggcaaccata tttcatatga cgcaattatt gaaaatactg gaaatgaact
      781 tccagattct tttgatatgc gtgtcgaaca tgagtatgac gaactgcgtg acaagttatg
      841 ggaagaagtt tcaaaactag ataagttaga tcaaaaaata ttgacgctat ataacgaaga
      901 atttacggaa cgggaaattg ctaaagaagt aggtctaagt caaaaggcgg taaaccttcg
      961 cattcgtaag tgcgtcgaac tcttgaaaga aaaattgaaa aattactaat ttaactactc
     1021 aaacgccgac tatttctcct tttaaaggta agaggctaaa aacctcaaag gagggaggta
     1081 gtatcggcga taagaaagag aagaacagtg aagagtataa ggaattaatc gaagccctgg
     1141 tactcatcag catggttaca gaagagttag ccaaaaaagt aattcgattg aaacaagaga
     1201 aaaaggagga aacgaatcat gagtaatttt gaacaagtag acaaattact agtaaaactc
     1261 atcgacgaga ttaatgcgtg gcgtaaagag ttaaaagaac tgtatgcgcc cgaaacaaaa
     1321 ggcacggaaa cagttcctga acaagccgtc gaattagtac cgattacgct cgtagaggta
     1381 cgcacaagac ttgctgaatt atcgcgagat gggaagacga atcaagtacg tgccttaatc
     1441 gagaaattcg gagcgtcgaa gttgtccgaa attgacccaa aacactatcc agcaattatg
     1501 gatgcggcgg tggaaattga tcatggcaag agataacgga cacgcattaa taggtggtag
     1561 ttcgctaagt cgggcaatgg aatgtccgcc cagtgtgcgc ctaacggaac actatgatga
     1621 tgaaagttcg atttatgcaa aagaggggac ttctgcgcac ttattactag aaaagaaggt
     1681 aaaaaggcga tacggaattc cctttgaaga aagtaatgaa gaacttgaat actatagcga
     1741 agaaatggaa gaaacgactg atatagcact taactttata agtgaagttt atgaaaagtt
     1801 agtggaagaa ggcaagcatc catttatcgc tagtgaagta ctcgtcgatt tctcggatgt
     1861 tgtccctgaa ggaagcgggt catcggacgt tgtgattgtt tatgatggtg gcatttatgt
     1921 tatcgactat aagcacggaa gaggtgtaga agtcagctgt gaaaataacc cacaattgat
     1981 gatttatgcc tacggaagta tgcgcatgtt tgaagcgctc tatgacacgg ttgacatcaa
     2041 catggtgatt atccagccgc gccttaataa catctcagaa tggtcatgta ctaaagatga
     2101 attagtggac tgggcagtga atgaagttaa gccgaaaaca gcgctcgcat acgaaggcaa
     2161 aggcgaattc aaatgtggtc cgtggtgtaa gttttgtaag gcacgacgag actgtcgaca
     2221 tcgagcagaa gagatgctta aaatcgaagg ctatcgtgtt aaagataaag cactactaac
     2281 cattgaagaa atcgctgaaa ttcttgggaa aattgatgac ttcataagtt ggggcaatga
     2341 catcaaagag tttgccctaa ctgaagcatt aagcggaaag caaataccag gattcaaact
     2401 cgtggaaggg cgctcaaatc gtaggtacac atccgaagaa gatgtcgtta aagcggtcga
     2461 agaagcagga cttgatccat tcgagaaaaa gttattatcc atcaccgaac taacgaaacg
     2521 ccttggtaag aagaagttta ctgaactcgt ggacaaatat gtctacaaac cagccgggaa
     2581 gccaactctc gtagaagaaa cagacaagag acccgtaata aacttggcaa gaattgattt
     2641 tatggaggaa taggaaatca tgtcaaaaac aaacccatgt aaggtaatta catcagtagt
     2701 gcgattcagc tacttaaacg caaatgaacc acgtgccaca acagaaggcg cagtaccaaa
     2761 gtattctgtt tcacttatta ttcctaaaac cgatacggat ttagtgaaac gcattcatga
     2821 tgcgattgaa gccgcctata aagaaggtga attcaaactt cgtggcgcta gtaaaagtgt
     2881 cccgccatta acagcaatta aaaatccact tcgtgacggc gatttagaaa gaccagatga
     2941 ccctgcttat aaaggtagtt actttgtaaa cgcaaactcg acgagtaaac ctgaagtagt
     3001 cgacgcagcg cttaatccaa ttattgaaac tagcgaaatt tatagcggca tttatgggcg
     3061 tgcctcaatt acattctacg cttttaacag caacggaagt aaaggcattg catgtggctt
     3121 aaatcacctt caaaagatcc gtgatggtga accattaggc tccaaagcaa cagctgaatc
     3181 cgacttcggt gacaacgaag gatttcttga ctaacaagaa taacttgggg agcggcacta
     3241 cgctgctctc ctttttggag gtatgtctat gattagtaaa ctatctatcg acttagagac
     3301 atactccgcg acatcattaa atgctggtgt ttataaatac accgaagatc cagcgtttga
     3361 aatattacta tttgcttatt caataaatga cggacctgta gaagtagtgg atttagcgat
     3421 gggcgacaaa gtaccacaaa ttgttcttga tgctttaatt aatcctaaca tcactaaatg
     3481 ggcttttaac gctcagtttg aaaggatatg tctctctcgc tttctaggct tacctgataa
     3541 tacttattta tctccacagg gctggaaatg ctcaatgaca tgggcggcta gtcttggtct
     3601 ccctttttca ttaaaggatg tagggaaagt cttaaacatc gataagaaga agattgaaga
     3661 agggaaagac ttaatacgat atttttgtct tccgtgtaag ccgataaaac aaaacgggca
     3721 tcgcttacgt aattactatt atcacgataa agaaaagtgg gaacaattta agttttataa
     3781 caagcgagac gttgaagcgg aaatggcgat tcaagaacga ttagcaaatc atccgatgtc
     3841 tgagtcttta tgggaggagt attggatgtg cgaattaatc aatgatcgcg gtgttttagt
     3901 tgatactatg ttatcgagga atgcgattaa aataaacggg atttgtcggg gaaaatacct
     3961 aaaaacactg caaaatatca ctgaattaga aaaccctaac tcggtcgctc
//
LOCUS       DAOSEX010000047         4007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_20458_length_4007_cov_3.047065, whole genome shotgun sequence.
ACCESSION   DAOSEX010000047 DAOSEX010000000
VERSION     DAOSEX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20458_length_4007_cov_3.047065"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PK612_01525"
     CDS             <1..550
                     /locus_tag="PK612_01525"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH94827.1"
                     /translation="AVMLLADGRTISKDVASYLFSGIVGDSGRFKYASTTSLTFGIAE
                     ILTSLGIDLNYIYNRLYIQNLDDLRCIGYILNNFKITKNGFAYYVMDEATLQELNITA
                     ERAKENVNVFADIRGIKVWAAITERTDTNEWRVSIRSAGIPINEFAQKWRGGGHVQAS
                     GASLLNREEIDVFVKEMDEYIG"
     gene            598..3585
                     /locus_tag="PK612_01530"
     CDS             598..3585
                     /locus_tag="PK612_01530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOH94828.1"
                     /translation="MSFIPLNVFTNYALLQSALTLDDYLKTLKNRNIKAAGVSDPDTL
                     FSYPHFAKKSREFHIKPLYGVRLNFEGSSLVIYPQNEKGYRELLALLEVKNKRELTLQ
                     DLKENSTNLIVIIPSSAIHNLELKEQIMQKYFYNFAINTTSLYIGLERENTELTAFLR
                     EFSDKYNYALVAFPLIKYAKSEDHKTLLMIEAIREGKSFSIYDISSGPDYILSDAELN
                     NYYSENELKNTHLISDLINFDLSVKHDLNFPLPGAYDPNKKIDELTLNALKAKGLDNN
                     KKYIERLTHELSIIRNLGYVNYFLIVSDYVNFAKKAGILVGYGRGSAPGSLVSYLLGI
                     THADPLEHNLLFERFLNPERVSLPDIDIDFMDIRRGEVIDYIKKTYGEDKVANIVTFQ
                     TNAAKASLRDTGNVFNIDPKFINRLSNSLGNTNYNFRDAYRNIKAFRDLVDNDTYNLE
                     IIKNATKIEGFPRQSGLHAAGIIIDGEPLAKNLPTFFNNGLRVTQYEMGFLEEHGFLK
                     VDILGLSNLTFVHNIISLVKENHHIDIDFYNLKFDDPRIYDVIRNGHTLGLFQLESAG
                     MRKAIATIKPTNFDDVSALLALYRPGPMKYIDEYALRKEVKSPITYINDDLIDILKPT
                     YGIMIYQEQIMQIAATMAGFSLGQADILRRAIGRKDEKIILEQKDAFFAGAKKRGYSR
                     KDTEKVFDDILRFADYGFNKSHSVVYAMLTTALAYLKTYYPYEFYQEILRGLVSDNVK
                     FSLARQELKALNIKISRPCVLKSTTEFIRTNNSINFPLSAINGLNRESANIIIRLREQ
                     KAFSSVQDFFFRSFKAGVTAEEFIALIEAGALDSFNTNRAQLKVMFNTYLPTLDIGLF
                     DNEAALHTIIVPEIKESDDRLDLEISRLRFPLSGNPLDKLKLKDIKRVSHILELEDGY
                     IKTYGVLTSLRLIKTKKGDEMAFATASDYEEEIEIVFFPRVLAEVKGMLTRNNIYIMA
                     GKLETRDGQKQLVVNTLERVQNE"
     gene            3578..>4007
                     /locus_tag="PK612_01535"
     CDS             3578..>4007
                     /locus_tag="PK612_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOH94829.1"
                     /translation="MSKRLIIIDGNSLLFRAYYATAYMGTDKIMRTSTGVPTNAIFAF
                     ANMIVRIVNDLKADDHLFVAFDTGEKTFRHEEYDDYKANRQAAPDELVTQFPIAREFL
                     DALSIRHDEVVGYEADDLAGSLAKAAGKEGFKVELYTSDQD"
BASE COUNT         1307 a          681 c          748 g         1271 t
ORIGIN      
        1 tgccgttatg cttttagcag atggccgcac tatatcaaaa gatgtcgcta gttatctatt
       61 tagtggtatc gttggtgatt ctggacgctt taaatatgcg tccactactt cacttacctt
      121 cggtattgcc gaaatcttaa caagtttagg cattgattta aattatatat acaatcgttt
      181 atacatccaa aacttagatg acttacgctg tattggttat atcttaaata attttaaaat
      241 tacgaaaaat ggttttgcct attacgtgat ggatgaggct acactgcaag aactcaatat
      301 tactgctgaa cgcgcaaaag aaaatgttaa tgttttcgct gatattcgcg gcattaaagt
      361 ctgggccgcc attaccgaac gcactgacac aaacgagtgg cgggtatcaa tacgcagtgc
      421 tggtataccg attaatgaat tcgcccaaaa atggcgaggc ggtggccatg tgcaagctag
      481 tggtgcttca cttttaaacc gtgaagaaat tgatgtattt gtgaaagaaa tggatgaata
      541 tataggctaa taagcctatt ttcatttctt gtctcttcaa tatgttaaaa taattatatg
      601 tcttttatcc ctttaaatgt atttacaaac tatgcgctcc ttcaaagtgc acttactttg
      661 gacgattatt taaagacact taaaaatcga aacatcaaag cagcgggtgt tagtgatcct
      721 gatacccttt ttagttatcc gcactttgcc aaaaaaagta gggaatttca tataaaacca
      781 ctctatggtg ttaggcttaa ttttgaaggt tcttcactag ttatttatcc tcaaaacgaa
      841 aagggatatc gtgaactttt agcgctcctt gaagtcaaaa ataaacgcga attaacgctg
      901 caagatttaa aagaaaatag cacaaattta attgttatta ttccctcttc cgctattcat
      961 aatttagagt taaaagagca aattatgcaa aaatattttt ataattttgc cattaataca
     1021 acatcattat acatcggttt agaacgggaa aatacagaat taacagcctt tttgcgagaa
     1081 tttagtgata aatataacta tgcactagta gcgttcccgc ttattaaata tgctaaaagt
     1141 gaagaccata aaacactttt aatgattgag gcaattagag aaggtaaatc gttttccatt
     1201 tatgatattt ctagtggtcc cgattatatt ttaagtgacg ctgaacttaa taattattat
     1261 agtgaaaatg aacttaaaaa tacgcatcta attagtgatt taattaactt tgatttaagc
     1321 gttaaacacg acttaaattt cccgcttcct ggcgcatatg atcctaataa gaaaattgat
     1381 gaattgacac taaatgcgct taaagcaaaa ggcttagata ataataaaaa atacatagaa
     1441 cgactcacac atgaattaag tatcattcgt aatttaggct atgttaacta cttcttaatc
     1501 gttagtgatt atgttaattt tgcaaaaaaa gctggaatct tagtcggtta cggtaggggt
     1561 agtgcaccgg gttcacttgt tagttatctt ttaggtatca cccacgctga tccacttgaa
     1621 cacaatttac tttttgaacg ctttttaaat cctgaacgtg tctctttgcc agacattgat
     1681 atcgacttta tggatattag acgcggtgaa gtaattgatt atattaaaaa gacttatggt
     1741 gaagataaag tcgctaatat tgtgactttc caaactaatg ccgcaaaagc atcgttgcgt
     1801 gatacaggta atgtttttaa cattgatccc aaatttatta atcgcttaag taactcttta
     1861 ggtaacacca actataactt ccgtgatgct tatcgtaaca ttaaagcatt ccgtgattta
     1921 gtggacaacg atacttataa cttagaaatt attaaaaatg ctactaaaat tgaaggattt
     1981 ccgcggcaaa gtgggcttca cgctgctggt attataattg acggcgaacc gctcgcgaaa
     2041 aacttaccaa cattttttaa taacgggtta cgcgttacac aatatgaaat gggttttctt
     2101 gaagaacacg gatttttaaa ggttgatatt ttaggactat caaaccttac ttttgttcat
     2161 aacatcatta gtttagtaaa agaaaatcat catatcgata ttgattttta taatttaaaa
     2221 tttgatgatc cgcggattta tgatgtaatc cgtaatggtc atacactagg acttttccaa
     2281 cttgaaagcg ccgggatgcg gaaagcaatt gcgacaatta aaccgactaa ttttgatgat
     2341 gttagcgcct tacttgcgct ttatcgtcct ggtccgatga aatatattga tgaatacgct
     2401 ctgcgtaagg aagtaaagtc accgataact tacatcaatg acgacttaat tgatatccta
     2461 aaaccaacat acggcattat gatttatcaa gaacaaatta tgcaaattgc cgcgacaatg
     2521 gctggcttta gccttgggca agccgatatc ttacgacggg cgattgggcg taaagatgaa
     2581 aaaatcatcc tagagcaaaa agatgcattt ttcgctggtg ctaaaaagcg aggctactca
     2641 agaaaagata ccgaaaaagt gtttgatgat attttacgct ttgctgatta cggatttaat
     2701 aaatcgcact ctgtagtgta cgcgatgctc actactgctt tagcgtatct taaaacttat
     2761 tatccttatg aattctatca agaaatatta cgcggtttag taagtgataa tgttaaattc
     2821 tccttagcga gacaagaatt aaaagcactt aacatcaaga taagtaggcc ttgtgtctta
     2881 aaatctacaa ctgaatttat tcgcactaat aactcgatta atttcccact tagtgcgata
     2941 aacggattaa acagggaatc cgcgaatatc attattagat tacgtgaaca aaaagctttt
     3001 agtagtgtgc aagacttctt cttccgcagt tttaaggctg gtgttaccgc tgaagaattt
     3061 atcgccctta ttgaagctgg ggcacttgac tcttttaaca ctaatcgcgc acagttaaaa
     3121 gtaatgttta acacctactt accaacttta gacattggct tatttgataa tgaagcggct
     3181 ttacatacga ttattgttcc tgagataaaa gagagtgacg accgtcttga tttagaaata
     3241 agtaggctgc gcttcccgct ttcaggtaat ccgcttgata aacttaaact aaaagacatt
     3301 aaacgcgtta gtcatatttt agaattagaa gacggttaca ttaaaactta tggtgtacta
     3361 acatcattac ggcttattaa gacgaaaaag ggtgacgaga tggcttttgc gaccgctagt
     3421 gattatgaag aagaaattga aattgtcttc tttccgcgtg tacttgcgga agttaaaggg
     3481 atgttaaccc gcaataatat ttatattatg gctggtaaac ttgaaaccag ggacggccaa
     3541 aagcaacttg tagttaacac tttagaaagg gtccaaaatg agtaaacgac ttattattat
     3601 agatggaaat tctttgcttt ttagagcata ttacgctact gcatatatgg gtactgataa
     3661 aattatgcgc acaagcactg gtgtaccgac aaacgccatt tttgcttttg cgaatatgat
     3721 tgtgcgcatt gttaatgatt taaaggctga cgatcattta tttgttgcct tcgataccgg
     3781 cgaaaaaaca tttaggcatg aagaatatga tgattataag gctaatcgcc aagcagcacc
     3841 cgatgaatta gtaactcaat ttccgattgc tcgtgaattt cttgatgcgc tttcgattcg
     3901 tcacgacgaa gttgtgggat acgaagctga tgacttagca ggaagtcttg ctaaagcggc
     3961 agggaaagaa ggttttaaag tcgaattata cactagtgat caagatt
//
LOCUS       DAOSEX010000048         3975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_20711_length_3975_cov_2.659184, whole genome shotgun sequence.
ACCESSION   DAOSEX010000048 DAOSEX010000000
VERSION     DAOSEX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20711_length_3975_cov_2.659184"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PK612_01540"
     CDS             <1..393
                     /locus_tag="PK612_01540"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOH94830.1"
                     /translation="HPSTNIDVANTQFTNTNVAITSFISSVTYLVMNSFVIDLINVRN
                     KKSQVQIITNDEELAKILISNFPHGATTIKGIGVYSQSEKSVIYMTVSSNEVNDVVRL
                     THQVDPKAFINVYDLRQVFGKFYISPIK"
     gene            complement(459..3401)
                     /locus_tag="PK612_01545"
     CDS             complement(459..3401)
                     /locus_tag="PK612_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009273554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH94831.1"
                     /translation="MLELVNIKRYYTQKKGPTVKALDDVSLVFPETGMVFVLGKSGSG
                     KSTLLNVIGGLDQATSGDLIIKGKSTKKFKQNEFDSYRNTMVGFIFQEYNILNDFTVG
                     QNIGIALELQGEKASNEKINAMLELLDMQGYGHRKPNTLSGGQKQRVAIARALVKNPQ
                     IIMADEPTGALDSVTGKQLFETLKKLSQDKLVIVVTHDHEFAENYGDRIIEFSDGKVI
                     RDVSKVKNEKQETNTVFSEEGITVSAGYELTAEDLVKINEYLRQNRDKTFIKVLNNDY
                     EFKPTKQPASFPKTEKLDLIKSKMPFKSALKIGASALKHKRFRLALSIILATSAFAMF
                     GLTDAIANFKRTETTINTMLDLNIGYSSISKIERIKYSDYYYSQQVGLDDDDLAELEK
                     IDNRLDFFPVLNEALEYSSNFYSDSFDYYDPYRYGINTIAHLKDADLTKANLSLLAGT
                     LPTNKNEVAVPEYVFDSFKKYGYRSLTNSTKIAIDDPEDLLNKTLNNKFVISGVYKTG
                     LEVDRFIIENVDDDGSIYNTINTEVFRQYLLNGFHTTLLVHEEYYNKLVEQNKVYSLP
                     FNSNSYFQMGDVDRYQFTTRNFRRIESLKTNNDVILFNGITHDNLGRDDIILSTSAAF
                     FQGMEGGVHDSVQTALLIFVDNIKSTDENDLAIEQMLKTNYQHYGFNSPLDVTAPSLW
                     SEQEKTSFRNFYAQALLTFLYDENPFQPELIGREAIFAVTSAQIKTDFAPFNIEYFLV
                     AYESDGEKLKAAQDLRVVGFTFDYQYYFEAWDSIIIYINEENAEVMNLTPEGAYFRAV
                     TYHGNSRAIIQKLVKFNELGTKKDVGSFYRMNNEVSYLVENVGDFLEIISRIFVVVGI
                     IFAVFASLLFLNFITLSVAFKKQEIGILRAIGARGIDVATIFMNEAGIIALINLILSF
                     MLAIIAGTTINNYIGKNINIYLSIVNVGLRQLLLLILIAFGVAFISSFIPVFRLSRKK
                     PIDAIRMR"
     gene            3515..>3975
                     /locus_tag="PK612_01550"
     CDS             3515..>3975
                     /locus_tag="PK612_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOH94832.1"
                     /translation="MRNKQLRILLAVTLTLNIILAVIKLGAGYLYNSPSLVGDGFNSF
                     FDIFISVMLLIVMKTASRAPDENHHYGHQKYEGLIFFGLGLFILFTTVVLGINTVKDF
                     IAYFQGAEYVLPGTPALIVAIVSVFIKVFMFIINYRAYLKLKRPALKGDAI"
BASE COUNT         1260 a          681 c          682 g         1352 t
ORIGIN      
        1 catccaagca caaatattga tgtcgctaat acacaattta cgaacactaa tgtggcgatt
       61 acaagtttta tttctagtgt cacttacctt gtaatgaatt catttgtaat tgacttaatt
      121 aatgtgcgga ataaaaaatc gcaagtacaa attattacaa atgatgaaga actcgctaaa
      181 attcttattt ctaacttccc acatggtgcg acaacaatca aaggtattgg ggtatattca
      241 caaagtgaaa agtctgttat ttatatgacg gtttcatcaa atgaagtaaa tgatgtagtg
      301 cgtttaaccc atcaagttga tcctaaagct tttattaatg tctatgactt gcgacaagta
      361 tttggtaaat tttacatatc accaattaaa taaagaatgt gaacattaaa gaaaatgcgc
      421 ttgcaaaaag cgcatttttt aatgtggaat aaatacttct agcgcattct aattgcgtcg
      481 attggtttct tacgacttag acggaagacc ggaataaagc ttgaaataaa cgctacacca
      541 aaggcaatga gaattagaag tagtaattgc cttaagccaa cattgacaat tgataaataa
      601 atattaatat ttttcccaat gtaattatta attgttgttc cagcgatgat ggcaagcata
      661 aatgagagta tgagattaat taaagcaata attcctgctt cgttcataaa gatagtagca
      721 acatcaatcc cacgtgcacc aatcgcacgt agtatcccaa tttcttgctt cttaaatgcc
      781 acacttagtg taatgaagtt aaggaagagt agtgaggcaa ataccgcgaa gataatgcca
      841 acaacgacaa atattctaga aataatttct aagaagtctc caacattttc aacaagataa
      901 gaaacctcat tattcatgcg ataaaaagaa ccaacatcct tttttgttcc tagttcatta
      961 aacttaacaa gtttttgaat aatagcacgt gaattgccgt gataagtaac tgcacggaag
     1021 taagcacctt caggtgttaa attcataact tcagcatttt cttcattaat ataaataata
     1081 atactgtccc acgcttcaaa ataatactga tagtcaaaag taaaaccaac aacacgtaaa
     1141 tcctgcgctg cttttagctt ttctccatca ctttcatatg caacaaggaa atattcaatg
     1201 ttaaatggcg caaagtcagt tttaatttgt gcgctagtta cagcaaagat ggcttcgcga
     1261 ccaatgagtt cgggttggaa cgggttttca tcatagagga aggtgagtag tgcttgggca
     1321 taaaaattcc ggaagcttgt tttttcttgt tcactccaga gagacggtgc agtaacatca
     1381 agcgggctat taaaaccata atgttgataa ttagttttta acatttgctc aattgcaagg
     1441 tcgttttcat ctgttgactt tatattatcg acaaatatta aaagtgcggt ttgaactgaa
     1501 tcgtggacac cgccttccat accttggaag aaagcggcag aagtgctaag tataatatca
     1561 tcccgaccta aattatcgtg cgttatgcca ttaaataaaa taacatcgtt gttagttttt
     1621 aaactttcaa ttctgcggaa gttacgggtt gtaaattgat aacgatcaac atctcccatt
     1681 tggaaataag aattagagtt aaagggcaaa gaatatacct tattttgttc aacaagttta
     1741 ttgtagtact cttcatgcac aagtagtgtg gtatgaaacc catttaataa atattgtcta
     1801 aaaacttcag tgttaatcgt gttataaata ctaccatcat catcaacatt ctcaattatg
     1861 aaacggtcaa cttctaaacc agttttatat acaccagaaa ttacaaattt attgttcaat
     1921 gttttattaa gcaaatcttc gggatcatca attgcaattt tagtgctatt agtcagtgag
     1981 cgatatccat actttttaaa ggaatcaaaa acatattcgg gaaccgcaac ttcgttttta
     2041 ttggtcggta atgtgcccgc taataagctg agattagctt ttgttaaatc tgcgtctttt
     2101 aggtgtgcaa tagtattgat gccataacga tatgggtcat aataatcaaa actgtcgcta
     2161 taaaaattgc tagagtattc aagtgcttca ttaagtacag gaaagaagtc tagtctatta
     2221 tcgatttttt ctaattcggc gagatcatcg tcgtctaacc ctacttgttg cgaataataa
     2281 taatcgctat atttgatgcg ttctattttg gaaatagagg agtatccaat atttaaatct
     2341 aacattgtgt taatcgttgt ttccgtacgt ttaaaattag caatggcgtc agttaaccca
     2401 aacattgcaa atgcactagt ggctaaaata atactaagcg cgagtctaaa tcttttatgt
     2461 ttaagtgcac tagcgccaat tttaagcgct gatttaaagg gcatctttga tttaatcaaa
     2521 tccaattttt ccgtttttgg aaaagatgct ggttgttttg tcggtttaaa ttcataatca
     2581 ttatttaata ccttaatgaa tgttttatcg cggttttgcc gtaaatattc attgattttg
     2641 actaaatctt ccgctgttaa ttcataaccc gcactaacag taattccttc ttcgctaaag
     2701 actgtatttg tttcttgctt ttcgtttttt actttagaaa catcacgaat tactttcccg
     2761 tcactaaatt caataatgcg gtcaccatag ttttcggcaa attcgtggtc atgagtcaca
     2821 acgatgacaa gtttatcttg tgataatttt tttagtgttt caaaaagttg tttaccagtg
     2881 acgctatcaa gtgcaccggt tggctcatcg gccataataa tttgcgggtt tttaacaaga
     2941 gcgcgggcga tcgctactcg ttgcttttgt ccgccggaaa gtgtatttgg tttgcggtgc
     3001 ccgtaaccct gcatatcaag taattcaagc attgcattaa ttttttcgtt tgatgctttt
     3061 tcaccttgta gttcaagcgc aatgcctata ttttgaccga cagtgaagtc atttaaaatg
     3121 ttgtattctt ggaagatgaa accgaccatt gtattgcggt agctatcaaa ttcattttgt
     3181 ttaaattttt tggtagattt acctttaatg attaagtccc cacttgtcgc ttggtcaagt
     3241 ccgccgatga cattaagtaa agttgatttc cctgaacctg atttacctaa tacaaacacc
     3301 attcctgttt ctggaaatac gagtgaaaca tcatcaagcg ccttaacagt tgggcctttc
     3361 ttctgcgtat aatatctttt tatattaaca agttctagca taataaaact ccttatcact
     3421 aatttctata ataaattata gcattatata gaaatatagt agttttatga ttaatcaatt
     3481 taattagcat ttgaaaagta ctataataca aaatatgaga aacaaacaac tacgcatcct
     3541 tttagcggtc acattgacct taaatattat ccttgcggtg attaagctcg gtgcgggata
     3601 tttatataat tcaccttcgc ttgttggtga tggctttaat tccttttttg atatttttat
     3661 tagtgtgatg ctacttattg tgatgaagac tgcgagtcgc gctcctgatg aaaatcacca
     3721 ttatggtcat caaaaatatg aaggacttat cttctttggt ttaggtctat ttattttatt
     3781 tacgacagta gtgttaggaa ttaataccgt taaagacttt attgcttact ttcaaggtgc
     3841 ggaatatgtt ttacctggca caccagcctt aattgtagcg attgtcagtg tctttataaa
     3901 agtattcatg tttattatta attatcgcgc ttatttaaaa ctaaagcggc cggctttaaa
     3961 gggggacgcg attaa
//
LOCUS       DAOSEX010000049         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_20944_length_3945_cov_3.044216, whole genome shotgun sequence.
ACCESSION   DAOSEX010000049 DAOSEX010000000
VERSION     DAOSEX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20944_length_3945_cov_3.044216"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PK612_01555"
     CDS             complement(<1..607)
                     /locus_tag="PK612_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOH94833.1"
                     /translation="MTIKDHIKHRFAAIYNTPPVAVYFSPGRVNLIGEHIDYHGGLVL
                     PVALSIGTYGAYSLRDDDEVRLYSTGYSDYPITINLGDLSKDSFTGWANYVRGVFQVL
                     KDEGYHVPHGLNLYLESDMPTSGGLSSSSSLELLIIKMLNDIFNFGIDRTKMAVLGKK
                     VENEYIGVLTGIMDQFVIANGKKNNALLLNTNTLKFDYIPLD"
     gene            775..1386
                     /locus_tag="PK612_01560"
     CDS             775..1386
                     /locus_tag="PK612_01560"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOH94834.1"
                     /translation="MVRKEIKQQAKDLIKNGKWFLLFVVLLIVSAVSSLTAGILMPVA
                     LFGLHLAIVGMFAGQEFDFNHFGTPFNDFNHALKVIAVYFLTMLIVMVGTMLFIIPGI
                     IFYMMYVQAYRIMIEKPEIGIMDALRESKEMMNGYKMDYFVFQLSFIGHFLLIMITFG
                     LWVIYLMPYFVVADTNYYLHLKQVRNEAKVVAEAAPVEAEVVE"
     gene            1477..2364
                     /locus_tag="PK612_01565"
     CDS             1477..2364
                     /locus_tag="PK612_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HOH94835.1"
                     /translation="MNKKQLEIMRSGKGFIAALDQSGGSTPKALRTYGVPETAYKTDE
                     EMFTKVHEMRTRIIKSDAFNSERILGAILFENTMDRKIDASYTADYLWEEKGIVPFLK
                     IDQGLCEPISGVQLMKPMPNLASLLERAKERKIFGTKMRSVIKEANEQGIKLIVEQQF
                     AVAKKVINYGLVPIIEPEVDINSPTKKEAETILLKFILEELAKLGPNDLVMFKVTLPT
                     MMNLYAPLLKDPRVVRVVALSGGYTLEHANDLLYLNKGVIASFSRALVADLRVDQSDK
                     DFDKTLAKAVESIYEASLT"
     gene            2385..3227
                     /locus_tag="PK612_01570"
     CDS             2385..3227
                     /locus_tag="PK612_01570"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HOH94836.1"
                     /translation="MNDLQKVLLENLKEFIRVCEKHNLRYYLVTGSALGAVRHKGFIP
                     WDDDIDVAMPRNDAMKLLALKDEFKEPYFLQHYTTDPSFPYPFMKLRNSATTFEEGLL
                     KFHKINHGIYIDIFIIDGMSKTSTLKNKWKNALMWPVMYLIYAANFWQKPRKNTFILQ
                     ILLYLVSILLLPFKINNWLIKALDKSMAKIPFEEATLVGAYLLWGGNKEAMDKRIYGP
                     GVKMKFETIEAVIPENYDAYLINRYGNYMELPPEHKRVGHHKPSALSTKVSYKDYYKR
                     KKRR"
     gene            complement(3253..>3945)
                     /locus_tag="PK612_01575"
     CDS             complement(3253..>3945)
                     /locus_tag="PK612_01575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH94837.1"
                     /translation="GDLVITPTPYSKKILDTYGLNREVVALSNGVDLARFNADNKEKI
                     QKFREYFKLKPDQKVVMTVGLFLARKGLHDLIEIAKDRPEYTFIWFGHTPKAVLSRKI
                     KKAIKNKPDNVIMPGYIAGDIIEGAFANADVFFFPTYEETEGIVVLEALASKCPLLIR
                     DIPIYEEWLTHGVNCHKGKTNEEFKALLDDIITKKAADTREAGYKVAAERDIKEIGKK
                     LGEIYRRVLEEK"
BASE COUNT         1269 a          669 c          739 g         1268 t
ORIGIN      
        1 ggtcaagcgg gatataatca aactttagcg tgtttgtgtt aaggagcagt gcattattct
       61 ttttcccgtt agcgataacg aactggtcca taatccctgt taaaacacca atgtattcgt
      121 tttcaacttt ttttcctaac acggccattt ttgtgcgatc aataccgaaa ttaaatatat
      181 cattaagcat tttaataata agcagttcaa gtgatgaaga agatgatagt ccgccacttg
      241 tgggcatgtc actttctaga tagagattaa gaccgtgtgg cacatgataa ccttcatctt
      301 ttaaaacttg aaacacaccg cgcacataat ttgcccaacc agtaaaggag tcttttgata
      361 agtccccaag attaattgtg attgggtaat cactatagcc agttgaataa aggcgaactt
      421 cgtcgtcatc acgcaagcta tatgccccgt acgtcccaat tgagagtgct acaggaagta
      481 caagcccgcc atgataatca atatgttcac caattaaatt aactcttcct ggtgaaaaat
      541 ataccgcaac tggtggagtg ttataaatag cggcaaagcg atgtttaata tgatctttaa
      601 ttgtcataag tttgtctcct ttgtgatgag attttaagta tatatcaaat atattttacc
      661 ctatttcatt tttaattact ctatgagtaa taattatcaa gtaatcacaa aaaattgaat
      721 tttaactacc gttaaattat tggataggtt ataatttaag aaggaggaca atttatggta
      781 agaaaagaaa ttaaacaaca agcaaaagac ctaattaaga acgggaaatg gtttttactc
      841 tttgtcgttc tcttaattgt tagtgcagtt tcaagtttaa ccgctggtat tttaatgcca
      901 gtcgcactct ttggattaca cttagcaatt gtaggtatgt ttgccggtca agaatttgac
      961 tttaatcatt tcggcacacc atttaatgat tttaaccacg cattaaaagt tatcgcggtt
     1021 tatttcttaa caatgctcat cgtgatggta ggtacaatgt tatttatcat tcctggcatc
     1081 atcttctata tgatgtatgt ccaagcctat cgtatcatga ttgaaaaacc agaaattggt
     1141 attatggatg cgcttcgtga aagtaaagaa atgatgaatg gctacaaaat ggattatttt
     1201 gtgttccaac ttagtttcat cggtcacttc ttattaataa tgattacctt tgggctatgg
     1261 gtaatttatt taatgccata ctttgttgtt gccgacacta attattactt acaccttaaa
     1321 caagttcgta atgaagcaaa agtcgtcgcc gaagctgcac cagttgaagc tgaagtagtc
     1381 gaataatttt taaaaattaa aactctcctg tgctaaaaca tatgtaattt aattattttc
     1441 ctttacaata tgtatagata gaggggagtt tttattatga ataaaaaaca attagaaatt
     1501 atgagaagcg ggaaagggtt cattgccgct cttgatcaaa gcggaggttc aacaccaaaa
     1561 gcattacgca cctatggtgt ccctgaaacc gcatataaaa ctgacgaaga aatgtttact
     1621 aaggtccacg aaatgcggac gcgaattata aaaagcgatg cctttaatag tgagcgaatt
     1681 ttaggggcaa tcttatttga aaatacaatg gaccggaaga ttgatgcaag ttataccgct
     1741 gattacttat gggaagaaaa aggtattgtt ccttttctta aaattgatca aggtctttgt
     1801 gaacctataa gtggtgtcca actgatgaag ccgatgccga atcttgctag tcttttagaa
     1861 agagcgaaag aacgaaaaat atttggtact aaaatgcgtt cggtcattaa agaagcgaat
     1921 gaacaaggca ttaaacttat tgtcgaacaa caatttgcgg tcgctaaaaa agtaataaat
     1981 tacggtttag tgccaattat tgaacctgaa gtggatatta atagtccaac gaaaaaagaa
     2041 gcagaaacga ttcttctaaa atttatcctc gaagagttag caaaattagg accaaatgat
     2101 ttagtgatgt ttaaagtgac tctacctaca atgatgaatt tatatgcacc acttttaaaa
     2161 gatccgcgtg ttgttcgtgt tgtagcttta agcgggggtt atactcttga gcacgctaat
     2221 gatttacttt atttaaataa aggtgtaatt gctagtttct cacgggcatt agtcgcggat
     2281 ttacgcgtcg accaaagtga taaagacttt gacaaaactt tagcgaaggc cgttgaatca
     2341 atttacgaag cttcgcttac ctaaataagg aaaagacatt aaggatgaat gatttacaaa
     2401 aagtattatt agaaaatcta aaagagttta ttcgtgtttg tgaaaaacat aatttacgct
     2461 attacttagt aacagggagt gcactaggtg ccgtaagaca taaaggcttt attccgtggg
     2521 atgatgatat cgatgtagcg atgccacgaa atgatgcgat gaaacttctc gcgctaaaag
     2581 atgaatttaa agaaccatac ttcttacagc actacacaac tgaccccagt tttccttatc
     2641 cgtttatgaa gttacgcaat agcgcgacaa cttttgaaga aggtctttta aagtttcata
     2701 agatcaatca cggtatttat attgatattt ttattattga tgggatgagc aagacatcaa
     2761 cccttaaaaa taaatggaaa aacgctttaa tgtggccagt gatgtatctt atttatgccg
     2821 cgaatttctg gcaaaaaccg cgtaaaaata catttatcct tcaaatctta ctctatcttg
     2881 tatcaattct tttattacca tttaaaatta ataattggct cattaaagca cttgataaaa
     2941 gtatggctaa aatccctttt gaagaggcga cacttgttgg tgcttacctg ctttggggcg
     3001 ggaataaaga ggcgatggat aaacggattt atggtccagg tgttaagatg aaatttgaaa
     3061 caattgaagc cgttatccct gaaaattatg atgcttattt aataaatcgt tatggtaatt
     3121 acatggaatt accaccagaa cataaaagag tcggtcacca taaaccgagc gccttaagca
     3181 ctaaagttag ttataaagat tattacaaac gaaaaaagcg ccgctagggc gccttttttg
     3241 tcgtttagtt aactattttt cttcgagtac tcggcgataa atttctccga gttttttacc
     3301 gatttcttta atgtcacgtt ctgctgctac tttgtaacct gcttcccttg tatccgcggc
     3361 ttttttagta ataatgtcat caagtaacgc tttaaattct tcattagttt tacctttatg
     3421 acaattgaca ccgtgcgtta accattcttc gtaaatcggg atatcacgga tgagaagtgg
     3481 acacttactt gcgagggctt caaggacaac aattccttct gtttcttcgt aagttgggaa
     3541 gaagaagaca tcagcgtttg caaaagcgcc ttcgataata tcaccagcaa tgtagcctgg
     3601 cataattaca ttatcgggtt tatttttaat ggctttttta attttacgac ttagaaccgc
     3661 tttaggggta tgtccaaacc aaataaatgt atattcaggc cgatcttttg caatttcaat
     3721 taagtcgtgg agacctttac gcgctaagaa aagcccaaca gtcattacga ctttttggtc
     3781 aggtttaagc ttaaaatatt cacgaaactt ttgaattttt tctttattat cagcgttaaa
     3841 tctcgctaaa tcgacaccgt ttgataaggc aacaacttca cgatttaagc cgtatgtatc
     3901 aagtattttt ttggagtatg gtgttggtgt tatgacaagg tcacc
//
LOCUS       DAOSEX010000050         3895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_21325_length_3895_cov_2.491667, whole genome shotgun sequence.
ACCESSION   DAOSEX010000050 DAOSEX010000000
VERSION     DAOSEX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21325_length_3895_cov_2.491667"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..96
                     /locus_tag="PK612_01580"
     tRNA            9..96
                     /locus_tag="PK612_01580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Leu,seq:taa)
     gene            188..427
                     /locus_tag="PK612_01585"
     CDS             188..427
                     /locus_tag="PK612_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOH94838.1"
                     /translation="MEDLKQTENKIKVLLNRIRPYIQSHGGDFEFVRFEEGFVYLKML
                     GACEGCPLVNQTLEYGIEEILMEEVPGVLGVLLDE"
     gene            complement(604..1254)
                     /locus_tag="PK612_01590"
     CDS             complement(604..1254)
                     /locus_tag="PK612_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94839.1"
                     /translation="MKRKGSLLILIIPMLFSLTACNSGLNEYRENAKAEIARFVTDWQ
                     HIFNDHQEALNIAVQKGEEAINKARNKKEIDSIVKNTKADIDALLINDDYEFSISTEM
                     VVKKSGEDFSVEVKLKNNSGADIEMSYFWSPFWPFAPRWQYPLFIEPSEFPETVTFLN
                     NETYTETWTLGANFEVGKHDLRFKALFYPNYYEDDTNCVVIWSPHIEIAVDYYLGK"
     gene            complement(1393..2838)
                     /locus_tag="PK612_01595"
     CDS             complement(1393..2838)
                     /locus_tag="PK612_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOH94840.1"
                     /translation="MKKKTIILMIPLLIFTLVASGCERGSESLSDNSRTGQKELSFYA
                     TNDFHGSIVETNSEPGIFKLGSYLKSEFAAKRNKTVYVDAGDIWQGSADSNINRGKFL
                     VEAMNVLKLDAQTIGNHEFDWYDTVIETNKELANYPFLGANIMVRETGELATNLVSYD
                     ETFKASTMVTRNDVNIGIIGTIGMLEGSILAGAIAPYTFKPVTTYIEREAENLRAQGA
                     DMIVLSTHDSLANSLGAYAPIIENKVVDIIFSGHHHVRDNQFINDVPIMQTEGNGKQV
                     MEVHGYYDFNLKEFKVTSREFTEAADIKRDYTEDTELLNLFNEKYAEEIERVKGEVIG
                     SLTNYINKSTLVKLANKVMYNFASITHNPDTIIAVHNYGGVRVNRIDAGEVTYGDIYK
                     AFPFDNEIKIFEDVLGSTASSFIDSNVSYPIGKFDIQNDQEYTIVTIDYVSDKKNSNI
                     RDYPHTVTGEYVRELIAEYFRTNGEIDPYTI"
     gene            complement(2835..3617)
                     /locus_tag="PK612_01600"
     CDS             complement(2835..3617)
                     /locus_tag="PK612_01600"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOH94841.1"
                     /translation="MKYKKELNAAIEAGKIARDIVLKIYESDFDVEIKDDNSPVTLAD
                     KMADKAMREYLGPLFPDYAFLTEESDDDLSRLTTDYVWIIDPVDGTKDFIHRDGEFTV
                     CIALTYKGEVVVGVIVVPVLNEIYYATKGGGAYELFADGSTKKLSVNDKTSNLTATGS
                     RHHLKPADNEYIEKHKDLISNVITYGSTLKAIKIAKGEIELFYRSGPGTKEWDIAAAD
                     IIVTEAGGIFIGPDGKKFTYNKADIHNRNGYLIVNRKENIRL"
     gene            complement(3607..>3895)
                     /locus_tag="PK612_01605"
     CDS             complement(3607..>3895)
                     /locus_tag="PK612_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH94842.1"
                     /translation="YLKLQELDILPSEGSNYVNTYSNLDYYPLWATFFIEKDGRVFVE
                     LRSKNYNVQKVATLFGGGGHLKASGCRLENEARIADVLQQLTKAEELKDEV"
BASE COUNT         1224 a          775 c          587 g         1309 t
ORIGIN      
        1 agagcatcgc ccgggtggcg gaacaggtag acgcaaggga cttaaaatcc ctcggttgtt
       61 gagaccgtgc cggttcaagt ccggccccgg gcaccagttc caaaagaatt gtcctactac
      121 ggtagggctt ttttattcat aagcattctt tttagtaaat ataatttcaa tttgttatac
      181 tatttgcatg gaagatttaa aacaaactga aaacaagatt aaagtactac ttaaccgcat
      241 cagaccttat attcaaagtc acggtggtga ttttgaattt gtaagatttg aagaaggttt
      301 tgtttattta aagatgcttg gcgcttgcga aggctgccca cttgttaatc aaacgctaga
      361 atatggtatc gaagaaatat tgatggaaga ggtaccaggc gttcttggcg tcttacttga
      421 tgaataaaag ctagtacaaa aagaaaaggt gcacttaaga tgcacctttt ttacattatt
      481 ccttactatt tataataaag ttttgtgtca cttgtcttaa aatctactgc tatcagtgta
      541 gtctaacagc cacttaagga gaaaagtatc cctacaaata gtacgcgttt tataaagcct
      601 cttttacttg cctaaataat aatccactgc tatttcaata tgcggcgacc atatcaccac
      661 acaattggta tcatcttcat aataattagg atagaaaagc gccttgaatc gtaagtcatg
      721 ctttccgact tcaaaattag cgcctaaagt ccatgtttcc gtgtatgttt cattatttaa
      781 gaaagtaact gtttcaggaa attcactagg ctcaataaaa agtgggtatt gccatctagg
      841 ggcaaagggc caaaatggtg accaaaaata tgacatttca atatctgcgc cactattatt
      901 ttttaattta acttcaacac taaaatcttc accgcttttt ttaacaacca tctctgttga
      961 tatactaaat tcataatcat catttattaa aagagcatca atatccgctt ttgtattttt
     1021 tacaatactg tcaatttctt ttttattcct tgctttatta atagcttctt cccctttttg
     1081 tacagctata tttaaagctt cttgatgatc attaaagata tgctgccaat cagtgacgaa
     1141 acgcgctatt tcagctttag cattttcgcg atattcgtta agaccactat tacacgctgt
     1201 tagactaaaa agcataggaa ttataagaat tagtaaactt ccttttcgtt tcataagatc
     1261 tcacagcctt tcattttagt tataatttgc ggcaatatta aagttttatc tctaccctta
     1321 ttttattaca aggtggggaa tttacaatac gaaaaaagct ataaagtatt ctaataaaat
     1381 taatttatct atttaaatag tgtaagggtc tatttcaccg ttagtgcgga aatattcagc
     1441 aattaattca cgaacatatt caccagtaac agtatgtggg taatcacgaa tatttgaatt
     1501 ctttttatca ctaacataat cgatagtaac gattgtatat tcttgatcat tttgaatatc
     1561 aaatttaccg attggataag aaacattgct atcaatgaaa cttgaagcag ttgagcctaa
     1621 aacatcttca aatatcttaa tttcattatc aaacgggaag gctttataaa tatcgccata
     1681 tgttacttct cctgcatcaa tcctgtttac tcgcacacca ccataattat ggacagcaat
     1741 aattgtatca gggttatgcg taatactcgc gaagttatac atcaccttat tagcgagttt
     1801 tacaagtgta ctcttattaa tataatttgt cagtgaacct ataacttcac cttttactct
     1861 ttcaatttct tcagcatact tttcattaaa taagtttaat aattcagtgt cttctgtgta
     1921 atctctttta atgtccgctg cttcggtaaa ttcacggcta gtaactttaa attcttttaa
     1981 gttaaagtca taatagccat gcacttccat tacttgttta ccattacctt ccgtttgcat
     2041 aattggcaca tcattaataa actgattatc acgcacatga tggtgaccgg aaaaaataat
     2101 atccacgact ttattttcga taataggcgc ataagcacca agactattag cgagtgagtc
     2161 atgcgttgat aagacaatca tatctgcgcc ttgtgctcgt aaattttctg cttcgcgttc
     2221 aatataagtt gttactggtt taaatgtgta aggggcaata gcacctgcta aaatggagcc
     2281 ttcaagcatc ccaatcgtcc caataatccc aatgttaaca tcgtttcttg ttaccatcgt
     2341 actagcttta aaagtttcat cgtatgacac taaattcgtg gcaagctcac cggtttcccg
     2401 caccatgata ttagcgccta aaaacggata attagcgagt tccttattcg tttcaataac
     2461 agtgtcatac caatcaaatt cgtggttacc aatagtttgc gcatcaagct ttaaaacatt
     2521 cattgcttca actaaaaact taccacgatt aatatttgaa tcagcactgc cttgccagat
     2581 gtcaccagca tcaacataca ctgttttatt tcttttagca gcgaattcac tttttaaata
     2641 tgaaccaagt ttaaaaatac caggctcact atttgtttca acaattgacc catgaaaatc
     2701 attagtagcg taaaaactta attccttttg cccagttcta gaattgtcac ttagagattc
     2761 acttcctctt tcgcatccgc ttgctacaag cgtaaaaata aggagcggta tcattaaaat
     2821 aattgttttt ttcttcataa tcgtatattt tccttccgat taacgattaa ataaccgtta
     2881 cgattgtgta tatcagcttt attataagta aacttcttac catcaggtcc aataaaaata
     2941 ccgccagctt cagtcacaat gatatccgct gcagcgatat cccattcttt agtgcctgga
     3001 ccactacgat aaaataattc aatttcacct ttagcaatct taatcgcttt aagtgtggag
     3061 ccgtatgtta ttacattact aattaaatct ttatgttttt cgatatattc attatcagca
     3121 ggttttaaat gatgacgaga accagtcgcg gttaagttac tagttttatc attaacactt
     3181 aacttctttg tcgaaccatc agcgaatagt tcgtaggctc ctccaccttt agtagcgtaa
     3241 tatatttcgt taagtaccgg cacaacaatg acaccgacaa caacttcacc tttataagtt
     3301 aaagcgatac acacagtaaa ttcaccatca cgatgaataa aatcttttgt gccgtcgacc
     3361 ggatcaataa tccacacata atccgtggta agccgagata aatcatcatc actttcttca
     3421 gttaaaaaag cataatcagg aaaaagtggt ccaagatact cacgcattgc tttatccgcc
     3481 attttatcgg ctaaagtaac aggagaatta tcatctttaa tttcaacatc aaagtcactt
     3541 tcataaattt ttaaaacaat atcacgcgct attttcccgg cttcaatcgc cgcatttaat
     3601 tcctttttat acttcatctt ttaactcctc agctttagta agttgttgca aaacgtcggc
     3661 aattcgtgct tcattttcta agcgacagcc acttgctttt aagtgaccac cgccaccaaa
     3721 aagcgtagcg accttttgca cattataatt tttactgcgt aattcaacaa acacacgccc
     3781 atctttttcg ataaaaaaag ttgcccataa cggatagtaa tcgagattcg aataagtatt
     3841 gacataatta ctaccttcac ttggtaatat gtctaattct tgtagtttta agtat
//
LOCUS       DAOSEX010000051         3870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_21541_length_3870_cov_3.485190, whole genome shotgun sequence.
ACCESSION   DAOSEX010000051 DAOSEX010000000
VERSION     DAOSEX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21541_length_3870_cov_3.485190"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..791)
                     /locus_tag="PK612_01610"
     CDS             complement(123..791)
                     /locus_tag="PK612_01610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH94843.1"
                     /translation="MLSLRLQAIANLIKKDDVVADIGSDHGLLVNYLAIKGHRALYAS
                     ENKKGPYNRLKSELKAYKSDIIEIDLADGLENLPARINTVVIAGMGGELIVSILQAHP
                     AKLVNVQKLILAPHCNAKAVREVLNALNYSISYEEIVVEKEVFYEIIVAEQKAIATKE
                     VNPYFGTYHLQARSENFIKMWTSVYNQNKRILSNKKLPLSRKEELLKEQELIEKVISE
                     GEKV"
     gene            complement(791..2242)
                     /locus_tag="PK612_01615"
     CDS             complement(791..2242)
                     /locus_tag="PK612_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH94844.1"
                     /translation="MDDKKLKDLAELEEVAIEEDDEVEIELTLPQIRKFLLEEARRNN
                     NELDQDDVIDATSHLDLSENEFDKLIQYFKNNKVTIHSDIESDEYDPDFANFVDGDDD
                     LDDDLDLDDDFDEEELSFGDAFKDVKEENFEENQRLRKISLGEVKTTDSVKIYMKDMG
                     RYSLLKADEEVTLARRYKEEGDMDARDELITRNLRLVFNEAKKYSSGNMHILDLIQEG
                     NIGLMKAVEKFDYTKGFKFSTYATWWIKQSISRSIADQARTIRIPVHMVETINKISRT
                     QRRLTQEFGRDATPEEISDALIKYSVKDVEEAMELSANATEENPIPEEVANKVRTIMA
                     KVETLTEELKRPATPEEVSESFREFTPARIREIQAIAMEPVSLESPIGEEDDSHLGDF
                     VEDKDNETPTDFVRKSILHDQLRDNMQEFLTPREERVLRLRYGFDDDKPRTLEEVGKI
                     FGVTRERIRQIEAKAIKKLRHPSRTKGLRGHIQ"
     gene            complement(2261..>3870)
                     /locus_tag="PK612_01620"
     CDS             complement(2261..>3870)
                     /locus_tag="PK612_01620"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOH94845.1"
                     /translation="RFVALYEKKTYYEAAQRLADMVSFKHDFFTKKQKFVVRDTKLDP
                     LISAINDLTAYYEYTLITEAGSEAKKYLVNRGLDDALLKKYKIGFAPRDGEATIKFLQ
                     SKGHALKTIEDIGILGGSLERPYDINHGRIIFPLNNKDGQVVAYSARALPSEKKAPKY
                     VNTRETQLFIKSDTLYNYHHAKALMAAQYVYLVEGFLDVIALDRVGERSVVALMGTAF
                     TKEQLALLRLLNKEVRIYLDSDTAGQMATLSVIKDLIAANIPLQIVKPHKDGYDPDDI
                     LNKEGKEALVTALTTFIPRDDFIFNYYLSVTKLDDMASTKKFVNDVMNEIILKLNSRL
                     EVSAMIERLAETSGFNSLVLTDMYESLRRKRQTRNEAINTLKQKLPVRQTLNKVAQAE
                     RFIVYQMLVYPEARTFFDNHVRVFTHEVHRYIVNYLKEFKPKEQVNYALLLNDIQSRF
                     TDEATINEYTQELLTIESGLQDVKYDENILNDAAKTLNYERELQILERKHQDELLRAP
                     DDASYAALKVKHIQAKTELMQKYGKKK"
BASE COUNT         1063 a          809 c          648 g         1350 t
ORIGIN      
        1 taacacaatg cgtctaactt cttcgacttt ttgccctgcc atatagccaa cataatcatt
       61 atgtttaagg gcgatacgct tagggtaaag acgaccaagt ttagttagta atgtgcgtgt
      121 cttcatactt tttctccttc actaataact ttttcaataa gttcttgttc ttttagcaac
      181 tcttctttac gacttaacgg taacttttta ttacttaaga tgcgtttatt ttggttataa
      241 acactagtcc acatcttaat aaagttttca cttctcgctt gtaagtgata agtgccaaaa
      301 tacggattaa cttcttttgt cgctatcgct ttttgctcgg caacaattat ttcgtaaaac
      361 acttctttct cgacaacgat ttcttcgtag ctaattgaat agtttaacgc atttaatact
      421 tcgcgcactg ctttagcgtt acagtgtggc gctaaaatga gtttttggac attaactaac
      481 ttagcgggat gcgcctgtaa gatacttaca attaattcgc cacccatacc agcgataacg
      541 acagtgttaa tgcgtgcagg taagttttca aggccgtcgg ctaaatcgat ttcaataatg
      601 tcactcttat aagctttaag ttcgcttttt aaccggttgt atggtccttt tttattttcg
      661 ctcgcatata aagcacgatg tcctttaatc gctaaataat taacgagtaa accgtggtca
      721 ctcccaatat cagcgacaac atcatctttt ttaattaaat tggcaattgc ttgtaaacgc
      781 agcgataaca ttattggata tgtccgcgta accctttcgt acgcgaagga tgacgtaatt
      841 ttttaatcgc tttagcttca atttgacgaa tccgttctcg tgtaacacca aaaatcttcc
      901 caacttcttc aagtgtccgc ggtttatcat catcaaagcc gtagcgtaag cgtaacacac
      961 gttcttcgcg tggtgtgaga aattcttgca tgttatcacg taattgatcg tgtaaaatgc
     1021 tctttctaac aaagtcggtt ggtgtttcat tatctttatc ttcgacaaaa tcacctaaat
     1081 gactatcatc ttcttcaccg attggtgatt ctaaacttac tggttccatc gcaattgctt
     1141 gaatttcacg aattctcgca ggtgtaaatt cacggaatga ttcacttact tcttctggtg
     1201 ttgctggccg tttaagttct tcagttaaag tttcaacttt cgccataata gtacgcactt
     1261 tattggcgac ttcttccgga atcggatttt cttcggttgc gttagcgctt aattccattg
     1321 cttcttcaac atccttaacg ctatatttaa ttaaagcatc actaatttct tcaggtgtag
     1381 cgtcgcgccc aaattcttgg gtaaggcggc gctgtgtccg gctaatttta ttaattgttt
     1441 caaccatatg aacaggaata cgaatagtgc gggcttggtc cgcgattgaa cgactaatcg
     1501 actgcttaat ccaccaggtt gcataagttg agaacttaaa accctttgtg taatcaaact
     1561 tttcgacagc tttcattagc ccaatattac cttcttgaat taaatcaaga atatgcatgt
     1621 tgccgctgct gtattttttg gcttcattaa aaacaagtcg taagttgcga gtaattaatt
     1681 catcacgcgc atccatatcg ccttcttctt tataacgacg ggcaagtgtt acttcttcgt
     1741 cagctttaag taaactatag cgccccatgt ctttcatata gattttaacg gagtctgttg
     1801 ttttaacttc accgagtgaa attttacgta accgttgatt ttcttcaaag ttttcttctt
     1861 taacatcttt aaaggcatca ccaaatgata attcttcttc atcaaaatca tcatcaagat
     1921 caagatcatc atctaaatca tcgtcaccat caacaaaatt agcgaaatct ggatcatatt
     1981 catcgctttc aatatcacta tgaatagtca ctttgttatt tttaaagtat tgaataagtt
     2041 tatcaaactc attttcgctt aagtctaggt gtgaagtcgc atcaataaca tcgtcttggt
     2101 cgagctcatt attattacgg cgcgcttctt caagtagaaa cttccgaatc tgcggtaatg
     2161 ttaactcaat ttccacttca tcatcttctt caatcgctac ttcctctaat tcagcgaggt
     2221 cttttaattt cttgtcatcc ataaactatc gtctccttta ctatttcttc tttccatatt
     2281 tttgcattaa ttcagtttta gcttgaatgt gcttaacttt aagggcggca taactcgcat
     2341 catcaggcgc acgtaagagt tcgtcctgat gtttgcgttc taagatttgt aattcgcgtt
     2401 cataattaag cgtcttagcg gcgtcattta atatattttc atcgtatttc acatcttgca
     2461 ggccactttc aatcgtgagt agctcttgcg tatattcatt aatagtagct tcatcagtga
     2521 aacgtgactg aatatcattt aataaaagcg cataattaac ttgctctttt ggtttaaatt
     2581 cttttaagta attaacgata tagcggtgta cttcgtgcgt aaacacgcgt acatggttat
     2641 caaagaaagt gcgtgcttcg ggatacacaa gcatttgata aacaataaag cgttctgcct
     2701 gcgcgacttt atttaatgtt tgccgcaccg gtaacttttg tttaagagta tttatcgctt
     2761 cgtttcttgt ttgtctttta cgccgtaagc tttcatacat atcagtgagc actaaactat
     2821 taaagccgct tgtttcagca agtctttcaa tcattgcact tacttcgaga cggctattta
     2881 gttttaagat gatttcattc ataacatcat taacaaactt ctttgtacta gccatatcat
     2941 caagctttgt aactgataag taataattaa aaataaagtc atcacgcgga ataaaggttg
     3001 ttaaggctgt tacaagcgct tctttccctt ctttatttaa tatgtcatca ggatcatagc
     3061 cgtctttatg tggttttacg atttgcagcg gtatattcgc tgcgattaaa tctttaatca
     3121 cacttagcgt cgccatttga ccagcagtgt cactatctaa ataaattctt acttccttat
     3181 taagtaagcg taaaagcgct aattgttctt tcgtaaatgc tgtccccatt aaggctacaa
     3241 cactgcgttc accaacgcgg tcaagagcaa tcacatcaag aaacccttcg acgagataca
     3301 catattgcgc tgccatcagt gcttttgcgt gatggtagtt ataaagtgta tccgacttaa
     3361 taaataattg tgtctcacgc gtattaacat attttggtgc tttcttttcg ctaggcaacg
     3421 ctcttgcgct ataagcgaca acttgtccgt ctttattatt aagcgggaaa ataattcgcc
     3481 cgtgattaat atcataaggt cgctcaagac taccacctaa aataccaata tcttcgatcg
     3541 ttttaagggc gtgaccttta ctttgtaaaa acttaatagt cgcttcaccg tcgcgcgggg
     3601 caaacccaat cttatatttt ttaagtaagg catcatcaag cccgcgatta acgagatact
     3661 ttttagcttc actacctgct tccgtaatta aggtatactc gtaataagca gttaaatcat
     3721 taatcgcact gataagcggg tcaagctttg tatcgcgcac aacaaacttt tgtttttttg
     3781 taaaaaagtc atgcttaaaa ctaaccatat ccgcgagccg ttgagcagct tcatagtatg
     3841 tctttttttc gtaaagcgct acaaagcgaa
//
LOCUS       DAOSEX010000052         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_21749_length_3848_cov_3.142368, whole genome shotgun sequence.
ACCESSION   DAOSEX010000052 DAOSEX010000000
VERSION     DAOSEX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21749_length_3848_cov_3.142368"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PK612_01625"
     CDS             complement(<1..759)
                     /locus_tag="PK612_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94846.1"
                     /translation="MRKNKWIALLISVLTLTGCKAVTETFEKGAFHTQDFSENYYTNI
                     PERYLTSNYEETVYEISNRININQPLDNAFDNAIRNDLVNQNLSLEEAIKLYGSAETK
                     AIKKEDFRNEIEYMEAIFNSLSSAQNDKASWINYAANNNLVKGKYSKAIQESFKRGIF
                     SKLTDGIIKCDGTGPKVRMQISEQGMGQTFEHELISYNRFVLSARGGTSVDYGSYGKA
                     RVTKAKVKINISFFIEKSTTNSASKHTVTFIVPEM"
     gene            complement(746..2134)
                     /locus_tag="PK612_01630"
     CDS             complement(746..2134)
                     /locus_tag="PK612_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94847.1"
                     /translation="MKDKIRESKLALISLFLALEVVLFIALDFGNIGPVFKALAFLLT
                     AILAPFFFEEMKVDLGQGLYVLLMPILFYALVTLVAPAYGSYPEMYSNEVFMQLPFIT
                     RLINLLGIVGLLLLGYFIGKSEVFATKHIFIVILAGLAAPVLISLFATLINYGFFHPL
                     VYKSLVTYYAGHEYEVSSQASLLYGFRIMVGDLKALASMAVIITSAGFGLLFVNEKKD
                     KFTIISLVVISAVGVLTLIFTASFAALIYLLPALIFALLLKFDLLKKLKNKVTLGVVI
                     ALVGLGAIIFLLTAYNIFNLQAVWQTNRVTRKLFFNGYMQRFYIIFKEAFGLRNLYGH
                     FAMTVEGYKVFPSGNFLFDAMWIDGLIGFVALLAFFVMFVIRLVKYFNKDKDERVLKV
                     VLISVLLTAFFRFMLRYPFKIFTYQEFSDINNFPLINSPLFLVTVFIAGYVFVLKEDK
                     KIEEEEVTHAEK"
     gene            complement(2134..3075)
                     /locus_tag="PK612_01635"
     CDS             complement(2134..3075)
                     /locus_tag="PK612_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94848.1"
                     /translation="MKRTRRSYIWEFLKSAIYVFRYKILRIKPPTNRIGKYKEITMKK
                     SNNAIEKAIRKGEPFAAIRFGAVEISALNNYEKIRLGFRRKYKKSVVYSIKNNAGVFP
                     PTQEIIDFYAKYLETNLKDTDYLGVSGVHMENYFFKHLTPNATPVQNWALDPLLGKWS
                     HLLAGKRVLVISPFAEQIEEQYEKRHLLFQAEPEILPEFTLVTIPAILTSGKAASKEF
                     DNWFEVLDYLKLEILKKDFDIALVGAGAYGTPLCLYIKSLGKMAIQSGGATQLLFGLM
                     GKRWEKREYVARHVNPHWSRPTVKPYGHKNIEGGCYW"
     gene            complement(3090..3776)
                     /locus_tag="PK612_01640"
     CDS             complement(3090..3776)
                     /locus_tag="PK612_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOH94849.1"
                     /translation="MENEKYKRLLQIISEEDLIKIQNKRIIIFGLGGVGGNALEALVR
                     SGFRRFTIVDNDVVDITNLNRQLIATTKTIGMPKVKVAKKRMLEIEPNCNINAVFEAV
                     TPENVANFNLEFYDYIVDAIDSFEDKMALIIYALKHNLPIISAMGAGNKIDPTKVIIT
                     KIEKTEGCPLAKKVRYRLRQEKLKGLMVVTSKELPLTNAGNKPGSSAFVPPAFGLAMA
                     SYILRNVISK"
BASE COUNT         1252 a          743 c          581 g         1272 t
ORIGIN      
        1 catctcaggg acaataaatg taacggtatg tttagaagca ctgtttgtcg tgcttttttc
       61 aataaagaaa ctaatattaa tttttacttt agctttagtg acacgcgctt taccgtaact
      121 tccataatca acacttgtac caccgcgagc gcttaacaca aaacggttat aactaataag
      181 ttcatgttca aaagtttgac ccatgccttg ttcactaatt tgcatcctaa cttttggacc
      241 agtgccgtca cacttaataa taccgtctgt taacttcgaa aaaattccgc gtttaaatga
      301 ttcttgaatt gctttactat attttccttt aacaagatta ttattagcgg cgtaattaat
      361 ccagctcgcc ttatcatttt gtgcactgct caaactatta aagattgctt ccatatattc
      421 aatttcatta cggaagtctt ctttttttat cgctttagtt tcagcactac cgtataactt
      481 aattgcctct tcaagtgata aattttggtt aactaagtca tttctaattg cattatcaaa
      541 agcattatca agtggttgat taatatttat acgattacta atttcataaa ctgtttcttc
      601 gtaattactt gttaaataac gttctgggat atttgtataa taattttcac taaagtcttg
      661 agtgtgaaat gcaccttttt caaaggtttc cgtaactgct ttacaacctg taagtgttaa
      721 gacactaata aggagtgcta tccatttatt tttccgcatg agttacttcc tcctcttcta
      781 tttttttatc ttcttttaag acaaaaacat accccgcaat aaagactgtc actaaaaata
      841 gtggtgaatt aatcagcggg aaattattga tgtcactaaa ttcttgataa gtaaaaattt
      901 taaaaggata acggagcata aagcggaaga atgccgttag taacacacta atgagtacaa
      961 cctttaatac tctctcatcc ttatctttat tgaaatactt cactaaacga atcacaaaca
     1021 tcacaaagaa tgcaagtaga gcaacaaacc caattaaacc gtcaatccac attgcatcaa
     1081 ataagaagtt acctgaaggg aatactttat aaccttcaac tgtcatcgca aagtgtccat
     1141 ataaattacg taatccaaat gcttctttga aaataatata gaagcgttgc atgtagccgt
     1201 taaagaataa tttacgcgta acgcgattag tttgccacac tgcttgtaag ttaaagatat
     1261 tataagcagt tagtaagaaa ataatagcgc caagtcccac tagggcaata acgacaccaa
     1321 gcgtcacttt gtttttaagt ttctttaaga ggtcaaactt aagtaacaaa gcaaaaataa
     1381 gcgctggtaa taaataaata agagccgcaa acgaggcggt gaatattaag gtaaggacac
     1441 caacagcact aataactacg agactaatga ttgtaaattt atctttcttt tcatttacaa
     1501 acaataagcc aaagcctgcg ctagtaataa taaccgccat cgatgccagt gcttttaagt
     1561 caccaaccat tattctaaag ccatacaata atgacgcttg actactaact tcatattcat
     1621 gtcctgcgta ataagttact aaacttttgt acactaacgg gtgaaaaaat ccgtaattaa
     1681 taagtgtagc aaaaagcgaa attaaaactg gtgctgctag tccggcaaga ataacaataa
     1741 atatatgttt tgtggcaaat acctcacttt tgccgataaa atagcccaat aataaaagtc
     1801 caacaattcc taacaaattt attaatcgcg taataaatgg tagttgcata aatacttcat
     1861 ttgaatacat ttcaggataa gaaccgtatg ctggtgcgac tagcgtcact aacgcataaa
     1921 aaagaatcgg cattaatagc acatataaac cttggcctaa atcaactttc atttcttcaa
     1981 aaaagaaagg cgcaagaatc gctgttaaca aaaacgctaa tgctttaaat accggaccaa
     2041 tattaccaaa atcaagcgca ataaaaagca ctacttctaa tgctagaaat aatgaaataa
     2101 gcgccaattt actttctcta attttatctt tcattaccag taacaccctc cttcaatgtt
     2161 tttatgacca tatggtttaa cagttggccg tgaccaatgt ggattaacgt gacgcgcaac
     2221 atattcgcgc ttttcccatc ttttacccat aagtccaaaa agtaattgtg ttgcaccgcc
     2281 actttgaatt gccattttgc ctaagctttt aatatataga caaagcggag tgccgtaagc
     2341 ccccgccccg acaagagcaa tatcaaaatc cttctttaaa atctctagtt ttaaataatc
     2401 aagtacttca aaccaattat caaattcttt acttgctgct ttaccagaag ttaaaatcgc
     2461 tggaatcgtc actagtgtaa attccggtaa tatttctggt tctgcttgaa aaagcaggtg
     2521 tcttttttca tattgttctt cgatttgttc ggcaaatggt gatataacaa gtacgcgttt
     2581 gcctgctaaa agatgtgacc atttaccaag aagcggatct agtgcccaat tttgcacggg
     2641 agtagcattt ggtgttaagt gtttaaagaa ataattttcc atatggacac cgctaactcc
     2701 taaataatcc gtatctttta agttagtctc aagatattta gcgtaaaaat caataatttc
     2761 ttgcgtaggc ggaaataccc cagcgttatt tttaatgctg tatacaacgg actttttata
     2821 tttccgtctg aaaccgagac ggattttctc ataattatta agtgcactta tttctacagc
     2881 gccaaatcga atcgccgcga aaggttcgcc tttccggatt gctttttcaa tcgcgttgtt
     2941 actttttttc atcgtgattt ctttatattt accgatgcga tttgttggtg gcttaattct
     3001 taaaatttta tagcgaaaaa catatattgc gctttttaag aattcccata tatatgagcg
     3061 acgtgttctt ttcatacatt tatcatacac tatttagata ttacatttct caaaatgtaa
     3121 cttgccatcg ctaatccaaa ggctggtggg acaaaggcac tacttcctgg tttattacca
     3181 gcattagtta atggtaattc ttttgaagta acaaccatta atcctttcag tttttcttga
     3241 cgtaagcgat aacgcacttt tttcgctagt ggacaccctt ctgttttttc tattttagta
     3301 ataattactt tagttggatc aatcttattg cccgcaccca ttgcactaat aataggcaaa
     3361 ttatgtttta aagcataaat aataagtgcc atcttatctt caaaactatc aatagcgtca
     3421 acaatataat cataaaattc taaattaaaa ttagcgacat tttcaggagt aaccgcttca
     3481 aaaacggcat taatattgca atttggctct atttctaaca tgcgcttctt tgccactttt
     3541 acttttggca taccaatcgt ttttgttgta gcaattaatt gtcgattaag attagtaata
     3601 tcaacgacat cattatcaac aattgtaaag cgtctaaaac cggagcggac aagcgcttca
     3661 agcgcattac caccaacgcc gccaagtcca aaaataatga tgcgtttatt ttggatttta
     3721 ataaggtctt cttcactaat tatttgtagt agtcttttgt acttttcgtt ttccatatat
     3781 aaactttctc atttcttcgg tgtagagtgc cttatttgat aaatatgatt cacaatgacc
     3841 cgcatcag
//
LOCUS       DAOSEX010000053         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_21792_length_3843_cov_3.085533, whole genome shotgun sequence.
ACCESSION   DAOSEX010000053 DAOSEX010000000
VERSION     DAOSEX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21792_length_3843_cov_3.085533"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PK612_01645"
     CDS             complement(<1..1229)
                     /locus_tag="PK612_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOH94850.1"
                     /translation="MKQRHKITILTLLALPLILGFTKWEKREKDPFITARAMPFVYPS
                     LNGEPKFSSQFHFNHINRGTVLNNYRGAGVTVAIIDSGLNTTHEDFLDGATTNISNKS
                     AYVEETGTYYSNIQIQSVEEHGRSIIEDTHPEAAGHGTNVASTVGAMVNGVGTAGISA
                     NVTLLVLKTNYMFTEINAAIYYAADNGADIINMSLGAYEESFIDGHGKQQNGIEGAST
                     YFQTAINYAYNKGVTVIAAAGNEKTDHYSYPASNNNVIGVGALGRNTSTTIASYSNFG
                     LHNVDIVSPGSVYVADIGGVSAYTETQGTSFAAPIVASAAAIYKGKYPTATPAKIEQR
                     LKETAYDLGAAGPDATFGYGRLDLSALLVDEVPVTGVEINPTSLTLRVGDTSQVSATI
                     LPENTSNKNHIFISENDA"
     gene            1380..>3843
                     /locus_tag="PK612_01650"
     CDS             1380..>3843
                     /locus_tag="PK612_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH94851.1"
                     /translation="MKKRLLSLFVISVLFSNVPSTSTKIVETNAQTGITVHYRGDYTN
                     PHIYYWNPQPAIITTPSWPGEKMIKDEGSDWYTKSWPELTAINLIFNNGGQPQTSDLS
                     RTTGEYWWVGQGTGGKWYTSDPDTITEPEYTDTGKLVIHVKSPHSVPKIKYYDSVPSG
                     ISTPNAINMTAEKEGWYRFGFNKISSIKAKFTIDGSESAEYFFTNGEWYLENGVLTDF
                     KPKVTSGKSDFREETIYFLMTTRFYDGDTSNNVHCWDDAKAGNLGSDPAWRGDFKGLI
                     QKLDYIKALGFSAIWITPIVENASGYDYHGYHALDFTQVDPRLESPNATYQTLINETH
                     ARGMKIIQDVVFNHSGNFGEKNIFPLFKKDYTKADTVSNLIKTDPHNLLGSDYDALDG
                     GRQYSRRINAMKEDANDFRHIYHHEKSLGWEDYNVQTGQIAGDCVDLNTENPYVSDYL
                     INAYNRYIDMGVDAFRVDTVKHISRLTFNNVFNPAFQNRGGDDFFMFGEVATRYRQVW
                     NNGNPAISTPFYTWKESKSYPWATREEREASTYQHWQDNANPATQPSSNNHLLDGNNY
                     RQVDYSKKSYMNVIDFPMHWNFKNANDAFNVAVGNDYTYADATWNVMYVDSHDYAQDG
                     APEGQRFAEPQSVWAENLSLMFTFRGIPTIYYGTEIEFQKGKPIDVGPNAPLSETGRA
                     YFGNHIEGSLTVTDFGKYTNANGAVATTLNHPLAKHIRNLNLIRRNIPALQKGQYSTA
                     GINGGLAFKRRFTDSTTDSFALVTISSSATFNNIPNGTYVDAVTGEAKVVTNGSLSVN
                     VSGKGNLRVFVLTTSKTPAPGKI"
BASE COUNT         1249 a          716 c          730 g         1148 t
ORIGIN      
        1 gcatcatttt cactaataaa gatatggttt ttattactag tgttttcagg aagaattgtt
       61 gcacttacct gactagtgtc accaactcta agtgttaaac ttgttggatt aatttcaaca
      121 ccagtaactg gtacttcatc aacaagtaag gcacttaaat caagacgacc gtaaccaaaa
      181 gtagcatctg gtcccgctgc acctaggtca taggccgttt cttttaatct ttgttcaatt
      241 ttagcaggag tcgcagtcgg atattttccc ttgtaaatag ccgctgcact tgccacaatt
      301 ggggctgcaa atgatgtgcc ttgtgtttcc gtgtaagcac taacgccacc aatatcagcg
      361 acgtaaacac ttcctggact aactatatca acattatgta agccaaagtt tgaataagaa
      421 gcgatggttg ttgatgtatt acgaccaagt gcaccaacac caataacatt attattactc
      481 gcaggataag aataatgatc agtcttttca tttcccgctg ccgcaattac tgttacacct
      541 ttattgtaag cataattaat cgctgtttgg aagtaggtag aagcaccttc gatcccattt
      601 tgttgtttcc cgtggccatc aatgaatgat tcttcatatg cgcctagtga catattaata
      661 atatcggcac cattatcagc agcgtaataa attgccgcat taatttcagt aaacatataa
      721 tttgtcttta acaccaagag tgttacattt gctgaaatac ccgctgttcc aacaccatta
      781 accatcgcac caactgtact agcgacattc gtgccgtgcc ccgcagcttc gggatgagta
      841 tcttcaatga tacttcgtcc atgctcttct acagattgaa tttgtatatt tgaataataa
      901 gttcctgttt cttcaacata cgctgattta ttagaaatat tcgtagtagc gccatctaaa
      961 aaatcttcat gtgttgtatt tagtcctgag tcaataattg ctactgtgac tcctgcacca
     1021 cgatagttat taagtacagt gccgcgatta atatgattaa aatggaattg actagaaaat
     1081 tttggttcac catttaaaga cggatacaca aaaggcatag cccgagctgt aataaacgga
     1141 tctttttctc ttttttccca tttagtaaaa ccaagaataa gtggtaaggc taacaaagtt
     1201 aggattgtta ttttgtgacg ttgtttcata gtaaaaatct ccagtaatta agataataac
     1261 atgaaactat tcaaaatata taaataataa gttttttgtg taacaaaaca ttattttcaa
     1321 cttaccaaaa taataatgtc atactttgtt ataatgaagt tagcattgga gaaacactta
     1381 tgaaaaaacg attgttatct ttgtttgtta ttagtgtcct ttttagtaat gtgccaagta
     1441 cttccactaa aatagtggaa acaaatgcac aaacagggat tactgttcat tatcgcggtg
     1501 actacactaa tccgcacatt tattattgga atccgcagcc cgctattatt actacaccaa
     1561 gttggcctgg tgaaaagatg ataaaagatg aaggaagtga ttggtacact aaatcgtggc
     1621 ccgaattaac tgctattaac ttaatattta ataatggtgg gcaaccacaa actagtgatt
     1681 tatcacgcac tactggcgaa tactggtggg tcgggcaagg cacgggtggt aaatggtaca
     1741 caagtgaccc cgataccatt accgaaccag aatacactga tactggtaaa ttagtaatcc
     1801 atgttaagtc accacacagt gtacctaaaa ttaagtatta tgacagtgtc ccaagcggaa
     1861 taagtacacc aaatgccata aatatgactg ctgaaaaaga gggatggtac cgctttggct
     1921 ttaataagat ttcaagtatt aaagctaaat tcacaattga tggtagtgaa agtgctgagt
     1981 actttttcac taatggcgaa tggtatttag aaaacggagt actcactgac tttaaaccaa
     2041 aagtaacaag tggtaaaagt gattttagag aagaaacaat ttacttcttg atgacaacaa
     2101 gattttatga tggtgacaca tctaataatg ttcactgttg ggatgacgct aaagcaggga
     2161 atttaggtag tgatccggcg tggcgcggag actttaaagg cttaattcaa aaacttgatt
     2221 acattaaagc ccttggtttt agtgcaattt ggattacacc aattgtcgaa aatgcttcgg
     2281 gctatgatta tcacggttat cacgctcttg actttactca agttgatccg cgcttagaaa
     2341 gtccaaacgc aacttatcaa actttaatta atgaaacaca tgcacgcggg atgaaaatca
     2401 tccaagatgt tgtctttaac cacagcggga actttggaga gaaaaacatc ttcccactat
     2461 ttaaaaaaga ttacacaaaa gctgacaccg ttagtaactt aattaaaact gacccgcata
     2521 atttacttgg tagtgactac gacgcacttg atggtggtag acaatatagt aggcgtatta
     2581 atgcaatgaa ggaagacgct aatgacttcc gccatattta tcaccatgaa aaaagtcttg
     2641 gttgggaaga ttataatgtg caaaccggac aaattgctgg agactgcgtc gatttaaaca
     2701 ctgaaaatcc ttatgttagt gactacttaa tcaatgctta taatcgctat attgatatgg
     2761 gagttgatgc ttttagagtt gatactgtta aacatatttc acgcttaacc tttaataatg
     2821 tttttaatcc tgcttttcaa aatagagggg gtgatgactt ctttatgttt ggggaagtcg
     2881 ccactcgtta tcgccaagta tggaataatg gtaaccccgc aattagcaca ccgttctaca
     2941 cctggaaaga aagtaaaagt tatccgtggg caacacgaga agagcgggaa gcaagtacat
     3001 atcagcactg gcaagataac gctaatccag caacacaacc tagcagtaac aaccatctgc
     3061 tagatggtaa taattatcgt caagttgatt actcaaagaa aagttacatg aatgttattg
     3121 atttcccgat gcactggaac tttaagaacg ctaatgacgc ctttaatgtc gcagtcggga
     3181 atgattatac ttatgctgat gcaacatgga atgtaatgta tgttgattcg catgactatg
     3241 cacaagatgg cgctcctgaa ggacaacgct ttgccgaacc acaaagtgta tgggcggaaa
     3301 atttaagtct gatgtttact tttagaggta tcccaacgat ttattacgga acagaaattg
     3361 aattccaaaa aggaaaacca attgatgttg gaccaaatgc cccacttagt gaaacaggtc
     3421 gcgcatattt tggtaatcat attgaaggct cactcactgt gactgatttt ggtaagtata
     3481 cgaacgcaaa tggagcagtt gcaacaacat taaatcaccc attagcgaaa catattcgta
     3541 acttaaactt aattagaaga aacatccccg ctttacaaaa gggacaatat agtactgctg
     3601 gtattaatgg cggtttagcg tttaaacgcc gatttactga tagcaccact gacagcttcg
     3661 cacttgtcac aatttcaagt agtgctacat ttaataatat tccaaatggt acttatgttg
     3721 atgctgttac tggtgaagca aaagttgtca ctaatggttc tttatccgtg aatgtatctg
     3781 gtaaaggcaa tttaagagtg ttcgttttaa ctacttctaa aacacctgca ccaggcaaaa
     3841 tag
//
LOCUS       DAOSEX010000054         3840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_21813_length_3840_cov_2.855746, whole genome shotgun sequence.
ACCESSION   DAOSEX010000054 DAOSEX010000000
VERSION     DAOSEX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21813_length_3840_cov_2.855746"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..630
                     /locus_tag="PK612_01655"
     CDS             256..630
                     /locus_tag="PK612_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94852.1"
                     /translation="MIPIRATSKKEASAKAKKTGVVKRDHPDWCLKGPTEITKEEYNE
                     LKAKLVNDPYWNKKTRQNTALFADKLIDEPNYTNRRGIKTNTVTFKKPTTAEIKMFHQ
                     KKRKIRDKEIQEFYDEANDYES"
     gene            complement(685..1251)
                     /locus_tag="PK612_01660"
     CDS             complement(685..1251)
                     /locus_tag="PK612_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94853.1"
                     /translation="MKRIHYYIVGTIILFATVIFSNSVGATSGANKRMEKLEVKARDL
                     FVTLSERYTKIEELHDNLSALDETATALVNNIEERITIFNDTKLLSQTVWPALQDAKY
                     IDENFMTLILYLKDNKDTFGSTLPSSFYTEFDAITSVLLPKINAYNSLTADYNTHIDL
                     FPNRLYLKGRNPYIIFDLALYPENLPLF"
     gene            complement(1266..>3840)
                     /locus_tag="PK612_01665"
     CDS             complement(1266..>3840)
                     /locus_tag="PK612_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94854.1"
                     /translation="DLMPSAENTNPTFVPFENPPSSEANRAELNYLKNVLVVPRDLET
                     VVFNKTIKLHFDFEFSGEGATNAFHVIDVTESDLPENFRFDTSAFPVPITYKLALIVP
                     VGESTILEGVTNINQIQTVLEDTPRDELVESIDQEPLNATMKVIARSAGKSKSKNFYF
                     NLTKPLFGAMVLEEVFKSGYLINLYRIHGFNQENNEVESTIENVADLPSESENPLNLS
                     YFLDSIAIPKIVTLKDLGDVTFEVTTNRPDLLFIGEMSKPIEDLDPGNELITGDVTVH
                     TLTPVGTYSSEIPASVDTVDKLKDHLESVDVKELYPYATQPTQDFSITITATCFGEER
                     TETYYFRLQEPPLDQEIVDYAIKSEYMINKVFYKDTTSIIRTTRENMPSTPEKRMKVS
                     YLTETLAIPRHVVLSDFGNKELDFSINVLGNNADKFYMSEQTDTLDGNPVTVKTLTPV
                     GGLAYTTPQNDLPAFAEEIKGLSREDLYDCVTQTSIDVDIELSATLNGKTATKTYYFT
                     LVAADIDVEIIDYLIDDSSLLNVVTYEDVNDDVKIQKIADQAKDPLNPYAIEHLVDTI
                     VVPQSITLTNFGNRELQIETTINSDDPGALNFYEASKSATISDNLVTAKTLTPLGSYD
                     ASAVDPNNLTALADSIKALPTKDLYKRSQSVGYNVELNVTVTLVSGEGEPEVRSTTFY
                     LKTIKADVDSKIGTAILDTYDIVNLIDHTDPYINVTNPPQPTDPLNPYQLLYFGETLV
                     FPIEFEYTDFENKLIQFDVNTNGKDHLFFISQKTHHYEGKDVEGITLSPIGTYDPGEP
                     LNTYQDLINHLKNDANIMAIAAARAVQVEENVTITVTISIDGREVAVNNYYFVLVVER
                     S"
BASE COUNT         1214 a          660 c          716 g         1250 t
ORIGIN      
        1 aatcatgtgg ccacatgatt ttattgtgtg tctacattta gggtatcagt acaatcacac
       61 ttatctttaa tataccatat taaacaaata tcacaactta aatttagcat gtgtattcaa
      121 aaatacgcct attagtgtta gaataagagt gaatttaata ttagttagga gtaattatgg
      181 ccttttttgc cgagcgcaaa tattatgttg ttacctgtaa atttggtcgc gttggtcgtg
      241 ataagtattt accacttgat acctattaga gcaacaagta aaaaagaagc aagcgctaaa
      301 gcaaaaaaga ctggggtggt aaagcgcgat caccctgatt ggtgtttaaa aggaccaacg
      361 gaaattacta aagaagaata taatgaactt aaagcaaaat tagtaaatga tccttattgg
      421 aataagaaaa cacggcaaaa caccgctctt tttgctgata agctcattga tgaacctaat
      481 tatacgaata ggcgcggcat taaaacaaat acagttacct ttaaaaaacc aacaaccgct
      541 gaaataaaaa tgtttcatca aaaaaagcgc aagattcgtg ataaagaaat ccaagaattt
      601 tatgatgaag caaatgatta cgaaagttaa aaaataaaat ccacctagtc tattagtaag
      661 gacactaaaa ggtggatttt tttgttagaa aagtggtaaa ttttctggat aaagcgctaa
      721 atcaaagata atatacggat ttcgtccttt taagtagagt cggttcggga aaagatcaat
      781 atgtgtgttg taatcagcag tcagactatt ataagcatta atctttggta ataaaacact
      841 tgttattgca tcaaactctg tgtagaagga agaaggaagg gtactaccaa aagtatcctt
      901 attatccttt aaataaagta tgagtgtcat aaagttttca tcaatatact tagcatcttg
      961 caatgcaggc cacacagttt gagagagaag cttagtgtca ttaaaaatag taatccgctc
     1021 ctcaatgtta tttactaaag cagtagcggt ttcatctaaa gcggataaat tatcatgtaa
     1081 ttcttctatt tttgtgtagc gctcacttaa ggtaacaaat aagtcgcgcg cttttacttc
     1141 aagtttttcc atgcgtttat ttgctccgct cgtagcgcca acggagttac taaaaataac
     1201 tgtcgcaaaa agaataattg ttccaactat ataataatga attcgcttca tttaatcgtc
     1261 tctttctaac ttcgctcaac aactaagaca aagtagtaat tattgacagc aacttcgcgg
     1321 ccatcaatcg atattgtaac agttattgtt acattttcct caacttgtac tgctctcgct
     1381 gcggcaattg ccatgatatt agcatcattt ttcaagtgat taattaagtc ttggtaagtg
     1441 ttaagtggtt cacctggatc ataagtgcca attgggctaa gtgtaatccc ttcaacatct
     1501 ttaccttcat agtgatgggt tttttgtgag atgaagaaca aatgatcttt accatttgta
     1561 ttaacatcaa actgaatgag cttattttca aagtcagtat attcaaattc aatcgggaac
     1621 acgagtgttt caccaaaata gagtaattga tatggattaa gcggatcagt tggttgtggt
     1681 gggttagtga cattaatata cggatcagtg tggtcgataa ggttaacaat atcgtaagta
     1741 tctaaaatcg ctgtcccaat cttgctatca acatcagcct taattgtttt taagtagaat
     1801 gttgtggaac ggacttctgg ttcaccctcg ccactaacta aagtaacggt gacattaagt
     1861 tcaacattat aaccaaccga ttgtgaacgt ttgtataagt cttttgttgg taaggcttta
     1921 atactatcag caagcgctgt taagttattt ggatcgacag cactagcgtc ataactacca
     1981 agcggtgtta gtgtcttagc ggtcactaga ttatcactaa ttgttgcgct cttactagct
     2041 tcatagaaat taagcgcacc aggatcatca ctattaattg ttgtttcgat ttgtaactct
     2101 ctgttgccaa agtttgtgag ggtaattgat tgcgggacga caattgtatc aacgagatgt
     2161 tcaatcgcgt aaggattaag tgggtcttta gcctgatcag caattttttg aatttttaca
     2221 tcatcgttta catcttcata agtaacgaca ttgagtaaag aactatcgtc aattaagtaa
     2281 tcgataattt ccacatcaat atctgcggcc acaagtgtga aataatatgt ttttgtcgcg
     2341 gttttaccgt taagtgtagc acttagttca atatcaacat caatactagt ttgggtaacg
     2401 caatcataaa gatcttcacg cgagagccct ttaatttctt cggcaaatgc tggtaaatca
     2461 ttttgcggtg tcgtgtaagc taaaccgccg actggtgtta atgtcttaac agtgacaggg
     2521 ttaccgtcaa gcgtatcagt ctgttcggac atataaaatt tatcagcgtt attacctaat
     2581 acattaattg agaagtctag ctctttgtta ccaaagtcac ttaaaactac atggcgcggt
     2641 atggctagag tttcggttaa gtacgatacc ttcatccgtt tttctggagt ggaaggcata
     2701 ttttcacgtg tcgtacgaat aatacttgtg gtgtctttgt aaaatacttt attaatcatg
     2761 tattcgcttt taattgcata atcaacaatt tcttggtcaa gcggtggttc ttgtagccta
     2821 aagtaataag tttctgtccg ttcttcacca aaacaagtcg cggtaatggt gatgctaaaa
     2881 tcttgagtag gttgcgtagc gtaaggatat aattctttta catccacact ctctaagtga
     2941 tcttttaatt tatcgacagt atcgacactt gcgggaattt cactactgta agtgccaaca
     3001 ggtgttaaag tgtgcacagt aacatcccct gtaattaatt cattaccggg atctaaatct
     3061 tcaattggct tactcatttc accaataaat aataaatcag gacgattcgt cgtgacttca
     3121 aaagtgacat cgcccaagtc tttaagtgta acaatttttg gaatggcaat tgaatcaagg
     3181 aaataagaaa gattaagtgg attttcgctt tcacttggta agtctgcgac attttcgatt
     3241 gtactttcaa cttcattatt ttcttggtta aaaccgtgaa tacgataaag attaattaag
     3301 taaccagatt taaatacttc ttcaagtacc attgctccaa ataatggttt agttaaatta
     3361 aaatagaaat ttttgctttt acttttacca gcggagcgcg caatgacttt catcgtggcg
     3421 ttaagtggtt cttggtcaat cgattctact aactcatcac gtggtgtatc ttctaaaacc
     3481 gtttgaattt gattgatatt agtaacacct tcaagaatcg tactttcgcc aacaggaaca
     3541 atcagcgcta atttgtaggt gatgggtact gggaaagcgc ttgtatcaaa gcggaagttt
     3601 tccggtaaat cactttctgt tacatcaata acatggaagg cgtttgttgc tccttcacca
     3661 ctgaattcaa aatcgaaatg tagtttaatc gttttattaa agacgactgt ttcaaggtca
     3721 cgcggaacaa ctaatacatt ttttaaatag ttaagttcag cgcggttcgc ctcactactt
     3781 ggtggatttt caaatggcac aaatgtagga ttagtgtttt ctgcactggg cattaaatca
//
LOCUS       DAOSEX010000055         3751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_22607_length_3751_cov_3.664773, whole genome shotgun sequence.
ACCESSION   DAOSEX010000055 DAOSEX010000000
VERSION     DAOSEX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22607_length_3751_cov_3.664773"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PK612_01670"
     CDS             <1..526
                     /locus_tag="PK612_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH94855.1"
                     /translation="AEDNKRGIELARQSEALGYPPADTVIGYCYFYGKGVEEDKQKGF
                     AYFHSGAVKGHDAAQYNMGIAYERGTVIPQDYALALEWYQRAADQKFAKAFYKLGYFH
                     EHGMGVTKDVTKAFSYYEKSANLGYVKAEVEVGKAHLSGRGLLRDLTKAREYLERAAA
                     KGNRIAARLLRDIE"
     gene            535..1521
                     /locus_tag="PK612_01675"
     CDS             535..1521
                     /locus_tag="PK612_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94856.1"
                     /translation="MTEKEKMVEVVEETPAETKTPQAFVNEEISDDATNEQLELPALE
                     TMRNTFSKMYRKFRIFSYVSTAIVIAVIVVSYVWLLPINQWAGIVPIILVLVASLVYS
                     RWARNKLTDNVRKYMADYNGYVNEYAVSETKVENYVYDFAGQLDKAAFTDARLLKDII
                     NSNSRNLVTYDLGRWHVEIADYAAYRRDGKQARAVFLGKLVTGTTEKEIDGRLVLYIK
                     PSEQKYDGGTGPDDLEDLELVVDKPEYKLYATNKELKKAISTKALEALLQVRPDNELA
                     DVSVALYKNKFAVTLTYSDALMVVPYKENVPVHAIKKYKEHLTALNKFIALL"
     gene            1578..2111
                     /locus_tag="PK612_01680"
     CDS             1578..2111
                     /locus_tag="PK612_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOH94857.1"
                     /translation="MSIKGKWIKIQSYKHDGNLHRFWRKSYVLEDNDEWLIVASRRTQ
                     VIEGNGRKWFTKEPAVTFFHKNDWFNVIAMLKTEGVVYYTNIASPTLIDYGMAKYIDY
                     DLDVKKFSDGTIKYLDKNEFLRNIENYQYSKELSAIVEYKQTQVVAMINEKTFPFDDE
                     EVRKLYNRFVEETKIKK"
     gene            2166..2627
                     /gene="tsaE"
                     /locus_tag="PK612_01685"
     CDS             2166..2627
                     /gene="tsaE"
                     /locus_tag="PK612_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019157052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOH94858.1"
                     /translation="MKHEFISISREQTVALGKRLAALFNLGDVVLLTGDLGAGKTTIT
                     KGIASGLNITEKVNSPTFNILKIYLKGDKPLFHIDAYRLEDDKKDIGLDEYIGEAGIT
                     VIEWPAFIKHLLPEKALEINITHRDLTSRNITLKGEGHYADVVKVIAEEFK"
     gene            2624..3205
                     /gene="tsaB"
                     /locus_tag="PK612_01690"
     CDS             2624..3205
                     /gene="tsaB"
                     /locus_tag="PK612_01690"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOH94859.1"
                     /translation="MITLLLDSSSHHLSVALAKGDKVLSFVEEKAFQTQSEIMTQRIN
                     ELLIKENISPNDIKRIVVASGPGSFTGVRIAVTIAKVWAYAKEVPLYAVSSLSVYRHE
                     TKPTICVLDARRERSFAGVYAQNNAILPDKIMQNSEILALAEKEGYLISGETKHLGIE
                     PAKFNRFENMLKSISEENRVKDIAAFKPLYLKG"
     gene            3210..3671
                     /gene="rimI"
                     /locus_tag="PK612_01695"
     CDS             3210..3671
                     /gene="rimI"
                     /locus_tag="PK612_01695"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOH94860.1"
                     /translation="METKEKKLIIRPMTIDDVDAVDELAFAALPDPWPKYTYAQELKN
                     EFAYLFVLTYADVILGFSHFWITFDSATIVKFAVHPAAQGKGLGSLLMSHLIERVSML
                     EEVRAITLEVRTSNDKAMHLYLKYGFNVVTKKRKYYEDGEDAYYMIKVLKE"
BASE COUNT         1299 a          549 c          783 g         1120 t
ORIGIN      
        1 tgcagaagac aataagcgcg gcattgaact tgcgcgccaa tcagaagccc ttggctatcc
       61 gccagcggac actgtgattg ggtattgtta tttttatggc aaaggtgttg aagaagataa
      121 acaaaaagga tttgcttact tccatagcgg agcagttaaa ggtcacgacg ctgctcaata
      181 taatatgggg atagcttacg agcgcgggac tgttattccg caagattacg ctttagcgct
      241 tgaatggtat caaagagcgg ctgatcaaaa atttgccaaa gctttctaca aactaggtta
      301 cttccatgaa cacgggatgg gtgtaacaaa agatgtgaca aaagcattta gttactacga
      361 aaagtctgca aatctcggtt atgttaaggc tgaagtggaa gttggtaaag ctcatttatc
      421 aggccgagga ctactacgag atctaacgaa agcccgtgaa tatttagagc gtgctgccgc
      481 gaaaggaaac cgtattgctg cgcgcttatt acgtgacata gaatagagga ttatatgaca
      541 gaaaaagaaa agatggtgga ggtagtggag gaaactcctg cagaaactaa aactccacaa
      601 gcatttgtta atgaagaaat tagtgatgac gctactaacg aacaattaga attaccagca
      661 ttagaaacaa tgcggaatac tttttcgaaa atgtatcgaa agttccgtat ttttagttat
      721 gtgtcaactg caattgttat tgctgtgata gttgtttctt atgtgtggtt actaccaatt
      781 aatcagtggg ctggtattgt tccaattatt ttagtacttg ttgcttcttt agtgtattca
      841 cgctgggcgc gtaacaaact tactgataat gtgcggaaat atatggcaga ttataatggt
      901 tatgtgaatg aatatgctgt gagtgaaaca aaagtagaaa attatgttta tgattttgct
      961 ggtcaattag ataaagcagc gtttactgac gctcgattac ttaaagacat tattaattct
     1021 aattctcgta acttagttac ttatgactta ggacgctggc atgtcgaaat tgctgattac
     1081 gccgcttata gacgcgatgg taagcaagca cgtgctgttt tcttaggaaa attagtgaca
     1141 ggaacgactg aaaaagaaat tgatggacga cttgtgcttt acattaaacc aagtgaacaa
     1201 aaatatgatg gtggcactgg tccagatgat cttgaagact tagaactagt cgtggataaa
     1261 ccagaataca aattatatgc aacgaataaa gaacttaaaa aagctatttc tacaaaagca
     1321 cttgaagcac tgttacaagt tcgtcctgat aacgaactcg ccgatgtaag tgtcgcatta
     1381 tataaaaata aatttgcagt tacacttact tatagtgatg ctttaatggt tgtgccatat
     1441 aaagagaatg tgccagtgca cgcaattaag aaatataaag agcatttaac agctttaaac
     1501 aaatttatcg ctttactata gtaaaaacat cagtttttat gttaaaatct aactaacaat
     1561 cgagagttga gttagttatg agtatcaaag gtaaatggat taaaatccaa agttataaac
     1621 acgatggtaa tttacatcgt ttttggcgta agtcttatgt tttagaagat aacgatgaat
     1681 ggttaatcgt cgcttcacgc cgtacacaag taattgaagg taacggtcgc aagtggttta
     1741 caaaagaacc agcggttacc ttttttcata aaaacgactg gtttaatgtg attgctatgc
     1801 ttaaaactga aggtgttgtt tattacacaa atatagcttc accgacactg attgattacg
     1861 ggatggcgaa gtatattgat tacgatttag atgtgaagaa atttagtgat ggtacgatta
     1921 aataccttga taaaaatgaa tttttgcgca atattgaaaa ctatcaatat tccaaagaat
     1981 tatccgcaat tgttgaatat aaacaaacgc aagtcgtagc gatgataaat gaaaaaacat
     2041 ttccgtttga tgatgaagaa gttaggaaac tttataatcg atttgttgaa gaaacaaaga
     2101 ttaaaaaata atatatttta attataaatt aaaacctaac taatcatggt ataattttgg
     2161 cgtctatgaa acatgaattt ataagtatta gtagagaaca aacagtcgcg ctaggtaaaa
     2221 gactagcggc attatttaat ttaggtgatg tcgtgctgct tactggtgac ttaggggctg
     2281 gtaaaacaac gattacaaaa ggaatagcga gcggtttaaa cattacagaa aaagttaata
     2341 gtccaacctt taatattctt aagatttatt taaaaggtga taaaccatta tttcatattg
     2401 atgcttatcg tttagaagat gataaaaaag atattggact agatgaatat attggtgaag
     2461 caggtattac tgttattgag tggccggcat ttattaagca tcttttaccc gaaaaggcac
     2521 ttgaaattaa tattacgcat cgcgatttaa cttcaagaaa cattacatta aaaggggaag
     2581 gtcattatgc ggacgtggta aaagttatcg ctgaggagtt caaatgatta cattactttt
     2641 agatagttcg agtcatcatt taagtgttgc gcttgcaaag ggcgacaaag ttttaagttt
     2701 tgttgaagaa aaggcttttc aaacccaaag tgaaatcatg actcaaagga ttaatgaatt
     2761 attaattaaa gaaaatattt caccaaatga cattaaaaga atagtggttg caagtggtcc
     2821 aggctctttt actggggtaa gaattgctgt aacaattgct aaagtatggg cgtacgctaa
     2881 agaggttcca ctttatgctg tttctagttt aagtgtttat cgccatgaaa caaaaccgac
     2941 catttgtgta cttgatgcaa gaagagagcg ttcttttgct ggggtttacg cgcaaaataa
     3001 tgccattttg ccggacaaaa taatgcaaaa cagcgaaata ttagcactag cggaaaagga
     3061 aggttactta atttcaggcg aaacaaaaca tttaggaatt gaacccgcaa agtttaatcg
     3121 ttttgaaaat atgcttaaaa gcattagtga agaaaataga gttaaggaca tagcagcgtt
     3181 taagccgctt tatttaaagg gataataaga tggaaacaaa agaaaagaaa cttattattc
     3241 gtccaatgac tatagacgat gttgacgctg ttgatgaact agcgtttgcg gcactccctg
     3301 atccgtggcc taaatatact tatgcccaag aattaaaaaa tgaatttgca tatctatttg
     3361 tcttaactta cgctgatgtg attcttggat tctcccattt ttggattacg tttgatagtg
     3421 ccaccattgt taaatttgct gtgcatcccg cggcgcaagg caagggacta ggaagtttac
     3481 taatgtcgca tctcattgaa agagtgtcaa tgcttgaaga agtaagagct attacattag
     3541 aagtaagaac gagtaacgat aaagcaatgc atctttattt aaaatacgga tttaatgttg
     3601 tgactaagaa acggaaatat tatgaagacg gtgaagatgc ttattatatg ataaaggtac
     3661 taaaagaatg aaagaacgaa taatattagc aattgaatct agttgtgata aaaccgctgt
     3721 tgctattatg cgtggtgaca aacttttaag t
//
LOCUS       DAOSEX010000056         3744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_22668_length_3744_cov_2.664950, whole genome shotgun sequence.
ACCESSION   DAOSEX010000056 DAOSEX010000000
VERSION     DAOSEX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22668_length_3744_cov_2.664950"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PK612_01700"
     CDS             complement(<1..962)
                     /locus_tag="PK612_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH94861.1"
                     /translation="MRILELNIDSFGKLENYNLKFDQKLTIINEDNSFGKTTIAQFIK
                     AMFFGMKTAKGKGKRLSLPRFKYLPWGKTTYGGNLTFVYNGRTYTVTRTFGESLSNDT
                     IAVKDLARNVNIDELNGIKITRDNLGDIIFGVNSESFEKLNFIQQLEVLYSSDEKIHD
                     DINVKLRGLFGHTTEDSDYTSAFNILDDAIKELSPGNQRRNSLYNKTDRELNATNNLV
                     FEAENAVNLISENSDELVALKRQKAENDKLKTKLENDLKLVASKKILEKDLKDYNALN
                     DEINNLNLQIEENKKLFAGNKVEDTNLVYLEKLSTDLSAKESEF"
     gene            complement(959..2020)
                     /locus_tag="PK612_01705"
     CDS             complement(959..2020)
                     /locus_tag="PK612_01705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HOH94862.1"
                     /translation="MKIIHTADIHIDSKMNRYGGDKTERRIREIKNAFFRMIDYGVKD
                     KVKVILIAGDLFDNKEVKLATLREVLTKITSAPTIDFIYLAGNHDDPAVFNHEKLAEL
                     PSNLKIVTKENNTFLYDDVCIKAYDNYADEDYTKTLIFDPNKYNILMLHGTEAEIPLN
                     NLKGKNIDYLALGDIHIPDIAAKRLDARGIYAYSGVLEPRGFDEVGERGFFVLEIQKN
                     KLIRREFIKNNSRTYHIIDVDISALNNTLEIELAIKKATNKIPSDDIVRLKLTGKYDY
                     ELIKDINALREYLESKFYYAELIDDSKVNLNTIDFANEVSLRGEFFRLIEASNYSPEV
                     KEELLKLGLNALKGRKIDL"
     gene            complement(2182..3516)
                     /gene="mgtE"
                     /locus_tag="PK612_01710"
     CDS             complement(2182..3516)
                     /gene="mgtE"
                     /locus_tag="PK612_01710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOH94863.1"
                     /translation="MKNLMILNDAALKKTLAKMHPYDIATKMKDASGDTQMRLIRLMA
                     LNKTVEVFLELPSEVSRHYFSLLTETQKKQFLEAFEMDDLKTFITSFEDDYQMYLLSL
                     LGTHKAIALKVLMSYDDEKIATIMSTNFVTITQNSTIKDTTAHIINSSKYNDFVDDIF
                     VINENNALIGSITLKDLISARATDTLDSITYSINNYLTLDDSLNDGYKKFENYAKSVL
                     PVLDDDMKIVGIVTADDVLNIMVAEHEEDIAKMTGVGDYDESSNAFRRSIQRMPWLVK
                     SVLLNLVIAALLSVFEKTLVEVIALIMFQPMILGMAGNIGTQSIAVTILKLNQDELAG
                     IHNEKKHIGKELAIGVLNSITIGLGGFLLSFLILSFINMGTHSPLLLAITIGTSLMGG
                     MFISAAAGVLIPIILEKMKVDPTIASGPIISTLNDLFALLIYFGIATLIFLL"
     gene            complement(3517..>3744)
                     /locus_tag="PK612_01715"
     CDS             complement(3517..>3744)
                     /locus_tag="PK612_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOH94864.1"
                     /translation="KVIGITEPFNVSLVVFISLAVTVATGPIFGLFIPLILDKMKIDP
                     AVASSPLITTLIDITSVVIYFGVATLLLGVI"
BASE COUNT         1167 a          664 c          558 g         1355 t
ORIGIN      
        1 ttaaattcac tctcttttgc cgacaaatcc gtgcttaatt tttctaaata cactaaattt
       61 gtgtcttcaa ctttattacc cgcaaataat tttttatttt cttcaatttg taaattaagg
      121 ttatttattt catcatttag tgcattataa tctttaaggt ctttttcaag tatcttttta
      181 ctcgcaacga gttttaaatc attttcaagt ttagttttta gcttatcatt ttcggctttt
      241 tgtcttttta gcgcaactaa ttcatcagag ttttcactaa ttaaattaac agcgttttct
      301 gcttcaaaca caagattatt tgtggcattg agttcacggt cagttttgtt atatagtgaa
      361 tttcttcgct ggttaccagg tgaaagttct tttatagcat catcaagaat attaaatgcg
      421 gaagtgtaat cactatcttc tgttgtatgc ccaaacagac cgcgtaattt tacattaata
      481 tcatcatgaa ttttttcatc actactatat aaaacttcta attgttgaat aaagtttaat
      541 ttctcaaaac tttcactatt tacaccaaag atgatatcac cgaggttatc acgcgttatt
      601 ttaataccat ttaattcatc aatatttaca ttgcgcgcta agtctttaac tgcaatcgta
      661 tcattagaga gggattcacc aaaggtgcgg gtaacagtat aagttcgccc gttgtagaca
      721 aaagttaagt taccaccata ggtggtttta ccccacggta aatatttaaa gcgtggtagt
      781 gatagtcttt ttccttttcc tttagcggtt ttcatcccaa agaacatcgc tttaatgaat
      841 tgcgcaatcg ttgttttacc aaaactatta tcttcattaa tgattgtaag cttttgatca
      901 aatttaagat tgtaattttc aagcttacca aaactatcaa tattaagttc taatattctc
      961 ataggtcaat tttccttccc tttagtgcat ttagacccaa ttttaagagt tcttccttaa
     1021 cttcagggga gtaatttgaa gcttcgatta ggcgaaagaa ttcaccgcgt aatgatactt
     1081 cgtttgcaaa atcaatagtg ttaaggttaa ccttagaatc atcgattaac tctgcataat
     1141 aaaatttact ttctaaatat tcgcgcagtg catttatatc tttgattaat tcataatcat
     1201 atttaccagt taattttaga cgtacgatat catcactagg aatcttatta gtggccttct
     1261 taattgctaa ctcaatttca agagtgttgt ttaatgcact gatatcgaca tcaataatat
     1321 gataagtacg actattattt ttaataaatt cgcgtctaat cagcttattt ttttgtattt
     1381 ctaaaacaaa aaagccgcgt tcacccactt catcaaaccc gcgcggttca agcacacctg
     1441 aataagcata tatgccgcgc gcatctaatc ttttcgcggc aatatcgggg atatggatgt
     1501 cgccaagcgc taagtagtca atatttttgc cttttaaatt atttaaaggg atttcagctt
     1561 cagtgccatg tagcatcaag atgttatatt tatttggatc aaagattaag gttttagtgt
     1621 aatcttcatc tgcatagtta tcataagctt taatacatac atcatcgtaa aggaatgtat
     1681 tattttcttt tgtcacaatt ttaagatttg aaggtaattc cgctaatttt tcatgattaa
     1741 atacagctgg gtcatcatga ttacccgcta aatatataaa atcaattgtt ggtgcacttg
     1801 taatttttgt taatacttca cgtaaggtag caagttttac ttctttattg tcaaataagt
     1861 ctcctgcgat taagataact ttaactttgt ccttaactcc gtaatcgatc atgcgaaaaa
     1921 aagcattttt aatttctcta atgcgtcttt ctgttttatc gccaccatac cgattcatct
     1981 ttgaatcaat atgaatatcc gctgtatgga ttattttcat acttaccccc gttaacttac
     2041 atttagttaa cttattttat cataatacct agccattaaa gtgaatatag cattaataaa
     2101 tgctattagc cttcattaat aaaaaaagac atcatgtggc gatgtctttt atattgtttg
     2161 taagcatctc ttctcttgct cttatagtaa gaagattaat gttgcaattc caaaataaat
     2221 tagtaatgcg aataagtcat ttagcgtcga gataattgga ccactagcaa tcgtaggatc
     2281 aaccttcatc ttctctaaaa taattggaat taaaacacca gcggcagcag agataaacat
     2341 cccgcccata agcgaagtcc caatcgtgat tgctagaagt aacgggctat gcgttcccat
     2401 attaatgaaa ctaagaataa gaaatgagag taaaaaacca ccaagaccaa tagtgatgct
     2461 atttaagact ccgattgcta gttctttgcc aatgtgtttt ttctcattat ggatacctgc
     2521 aagttcatct tgatttaatt ttaaaatcgt cacggcaatt gattgagtgc caatgtttcc
     2581 tgccatccct aaaatcattg gttgaaacat aattaaggcg atgacttcaa cgagtgtctt
     2641 ttcaaatact gataataggg cagcaatcac taagtttaat aatactgatt taactaacca
     2701 cggcattctt tgtatagaac gtctaaaggc attggatgat tcatcataat caccaacccc
     2761 tgtcatctta gcgatgtctt cttcatgttc agcgaccatt atatttaata catcatcagc
     2821 agttacaatc ccgacaattt tcatatcgtc atctagtact ggtaagacac tttttgcata
     2881 gttttcaaac tttttataac catcattaag tgagtcatca agtgttaaat aattgttaat
     2941 gctataagtt atgctatcaa gtgtatcagt ggcgcgtgca ctaattaaat cttttaaagt
     3001 tatacttcca attaaagcat tattttcatt aattacaaag atatcatcga caaaatcatt
     3061 gtattttgag ctattaatga tatgcgcagt agtatctttg atcgttgaat tttgtgtaat
     3121 cgttacaaaa ttagtgctca ttattgttgc aattttttcg tcgtcataac tcattaaaac
     3181 tttaagtgct atagctttat gtgtccctag taaacttaat agatacattt gatagtcgtc
     3241 ttcaaaacta gtaatgaatg tttttaaatc atccatttca aaagcttcta agaattgctt
     3301 tttttgtgtt tccgttaata aactaaagta gtgacgcgaa acttcacttg gtaattcaag
     3361 gaatacttca actgttttat ttagtgccat caaacgaatt aatcgcattt gcgtatcgcc
     3421 ggaagcatcc ttcatttttg tcgcgatgtc ataaggatgc attttcgcta aagttttctt
     3481 taaggcggca tcatttaaaa tcattaagtt tttcatttaa atcaccccca gtaataatgt
     3541 tgctaccccg aaataaataa caacactagt aatatcaata agtgtagtaa taagtgggct
     3601 agaagcgact gctggatcaa ttttcatctt gtctaaaata agcgggatga ataatccaaa
     3661 tattggtcca gttgcgaccg tcaccgctag tgaaataaag acgactaatg atacattaaa
     3721 gggttcagta atcccaatta cttt
//
LOCUS       DAOSEX010000057         3724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_22820_length_3724_cov_4.347779, whole genome shotgun sequence.
ACCESSION   DAOSEX010000057 DAOSEX010000000
VERSION     DAOSEX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22820_length_3724_cov_4.347779"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK612_01720"
     CDS             complement(<1..277)
                     /locus_tag="PK612_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94865.1"
                     /translation="MSVFKALLINKMNINDDTLILSENDTVYHIFDENDLPTSVTKVI
                     LKSSKDIDFVVVLRQNKKFITYELAPYTRGKVMFMCSEENLSIDREII"
     gene            complement(274..1086)
                     /gene="sufC"
                     /locus_tag="PK612_01725"
     CDS             complement(274..1086)
                     /gene="sufC"
                     /locus_tag="PK612_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOH94866.1"
                     /translation="MVLIEIKDLHASVNDIEILRGVNLKINRGEVVALLGPNGHGKST
                     LLQVIMGHPNYKVTKGEILYKGQDVLAMSVDERARAGLFLAMQNPSEVPGVQISDFLR
                     AAINTKREKPIKPIELFRVIDKASKEMQMPHDLASRNLNEGFSGGEKKRHEILQMKLL
                     KPEFAMLDEIDSGLDVDGIRIVSQQINEEKKDKTFLVISHYARLYELINPTRAIVMIN
                     GRVAIEGGMDVIKRIDQEGYEWIQVEHNINIEREDKRPIVLEICAVNERSKQ"
     gene            1414..3540
                     /locus_tag="PK612_01730"
     CDS             1414..3540
                     /locus_tag="PK612_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOH94867.1"
                     /translation="MKTTLIIPNIGCPNCAMAITEHFKTLNIEAKVNVNQKKVTFTYS
                     DKDSFTIILKELKAIGFPAAPIDYDEKRRRKYEKVRLIVATILVLPLAYTMFDNFGLN
                     IVPDIMLNGYFQMGFAAILQFIFGFRFYKNAFYQIKNKNLGMDVLIVLGTSVAFFYSL
                     YLTIFTDKTQLFFETSGMLIWMVTIGDYLEHLSQAKTTDTLKSLMALAVDEVRLIKNG
                     EEQIVSLNDVNVGDVIKVLAGEKIALDGEIIDGASYIDDSVITGESIPKYLSVGDTAV
                     GSTTNLSNTILIKVSAIGSDTVLAKIIKTVEETSLIKPKFQKAVDVIAKYFVFIILMI
                     ALVSFLVYQFILGKELSISLEVTTAVLVVSCPCALGLATPTSIAVASGLAFKNKILYK
                     GGEFFELANKIDAIAFDKTGTLTKGDLIVSDYLGDIRYLSYTKGLEIHSTHPIGKSIN
                     NYRKDIIPFAIEEYEIIDGKGIKGTYLQDQIIVGSATFIAEANISNPFLNEFEKITGE
                     GKVVIFTAINKKVVNMIILEDEIKAESQTLITALKKMNINVYLITGDNENTAKYLANK
                     VGIENYYSGVLPNEKAHIIKKLQEKHSVVAFVGDGINDAPALKQADVSFSVSNASDIA
                     TDTADVTLLDNDLKLVLIAIDLSKKTTANIKQNIIWAFLYNLVMIPLAIFNIATPLVG
                     GVLHVISSLLVVFNALRLNLYKHKIK"
BASE COUNT         1228 a          598 c          577 g         1321 t
ORIGIN      
        1 aaataatttc gcgatcaatg ctaagatttt cctcactaca cataaacatt acttttccgc
       61 gcgtatatgg cgcaagttca taggtaatga actttttgtt ttgccgtaat acaacgacaa
      121 aatcaatgtc ttttgatgat tttaatataa ctttagtcac agaagtaggt aagtcgtttt
      181 cgtcaaaaat atgataaaca gtatcgtttt cacttaagat gagcgtgtca tcattaatat
      241 tcattttatt tattaataac gctttaaaaa cactcattgt ttacttcttt cattgaccgc
      301 acatatttct aatacaattg ggcgtttgtc ttcgcgttca atattaatgt tgtgttctac
      361 ttgaatccat tcataacctt cttggtcaat gcgcttaatg acatccatac cgccttcaat
      421 cgctactcga ccattaatca ttacaatggc gcgcgttgga ttaataagtt catatagtct
      481 tgcgtagtga ctaataacga gaaatgtttt atcttttttc tcttcattta tttgttgcga
      541 gacaattcga atcccgtcaa catcaagacc cgaatcaatt tcgtccaaca ttgcaaattc
      601 aggttttaat aatttcattt gcaaaatttc atgacgcttc ttctctccgc cagaaaaacc
      661 ttcatttaaa ttacgcgagg caaggtcgtg gggcatttgc atttctttgc ttgctttatc
      721 aatcacgcgg aaaagttcaa ttggtttaat tggtttttca cgttttgtat tgatagcggc
      781 gcgtaagaaa tcactaatct gtacaccagg aacctcactt gggttttgca ttgcaagaaa
      841 aagccctgct ctagctcttt cgtcaacact cattgctaaa acatcttgac ctttatataa
      901 aatttcacct ttcgtaactt tataattagg gtgacccata attacttgta gtaaggttga
      961 tttgccatga ccattaggac caagtaaggc cactacttcg ccacggttaa tttttaagtt
     1021 aacaccacgt aatatttcaa tatcatttac ggatgcatgt agatctttaa tttcgattaa
     1081 taccatcttt acaaactctc tttccgttgt atgcatataa aagatacctt aaaacaccta
     1141 aaaaataaac tgttttacta tcctattttt atttaaaaat aacatatcat tgttttatat
     1201 gcttatttat taatttttat ttcagtaaaa aaggatttta tattccctaa catccttagt
     1261 tttatcattt attgcaatgt ttatgtttca cctccgttca tcattattta tttcattcaa
     1321 gtaaactatc ctaacaacaa ttagtattac ttgaattagt tttattatac tattataatt
     1381 atagtgttag caacggctaa ctggtaagga aatatgaaaa caacattaat aatcccaaat
     1441 attggttgtc cgaattgtgc catggcaatt actgagcact ttaaaacatt aaacatcgaa
     1501 gcaaaagtaa atgtcaatca gaaaaaagtg acctttacat atagtgataa agattctttc
     1561 accatcatat taaaagagtt aaaagcgatt gggttccctg ctgcgccaat tgattacgat
     1621 gaaaaaagac gtcggaaata tgaaaaagtc cgtttaatag ttgcgacaat tttagtgtta
     1681 ccacttgcat acacaatgtt tgataatttt ggattaaaca ttgtccccga tattatgcta
     1741 aacggctatt tccaaatggg ttttgctgca atattgcaat ttatttttgg atttagattt
     1801 tataaaaatg ctttttatca aataaaaaat aaaaatttag gaatggatgt tttaatcgtt
     1861 ttaggtactt cagtagcgtt cttttatagt ttgtatttaa ctatttttac agataaaact
     1921 caattgtttt ttgaaacgag cggtatgctt atttggatgg taacaattgg tgattattta
     1981 gaacatctct cacaagcaaa aacaacggat acattaaaat cattaatggc acttgcggtt
     2041 gatgaagtta ggcttataaa aaacggagag gaacaaatag tttcacttaa tgatgtgaat
     2101 gttggcgatg ttataaaagt gcttgccggc gaaaaaatcg ctttagatgg tgaaattatt
     2161 gatggcgcta gttatattga tgattcagtt ataacaggcg aatcaatccc taaatatctt
     2221 agtgtaggtg ataccgctgt agggtctacc actaatttat ctaatacaat tttgattaaa
     2281 gtaagtgcta tcggcagtga tactgtacta gctaaaatta ttaaaacggt tgaagaaact
     2341 tcgcttatta agcccaaatt ccaaaaagct gtggatgtta ttgctaaata tttcgttttt
     2401 attattttaa tgattgccct tgtatcattt cttgtttatc aatttatttt agggaaagaa
     2461 ctctcaattt ctctcgaggt gactactgcg gtcttggtag tttcatgtcc ttgtgctctt
     2521 gggttagcaa caccaacttc aattgcagtt gcaagcggat tagcatttaa aaataaaatt
     2581 ctatataaag gcggtgagtt ttttgaactc gccaataaaa ttgatgcgat tgcttttgat
     2641 aaaacaggaa cccttactaa aggtgattta atagtcagcg attatcttgg cgatattaga
     2701 tatttaagtt atacaaaagg cttagaaatc cattccactc atccaatcgg aaaatctatc
     2761 aataactatc gaaaagatat tattcctttt gcaattgagg aatatgaaat aattgatggc
     2821 aaaggtatta aaggcacata tttacaagat caaattatag tgggttctgc tacttttatt
     2881 gctgaagcta atattagtaa tcctttctta aatgaatttg aaaaaattac aggtgaaggt
     2941 aaggtcgtca tctttactgc gattaataaa aaggtcgtaa atatgattat tttggaagat
     3001 gaaataaaag ctgaaagtca gactttaatt acagcactta aaaagatgaa catcaatgtc
     3061 tacctaatta ccggcgataa tgaaaatacc gctaaatact tagcaaataa agttggaatt
     3121 gagaattatt atagtggtgt gttaccgaat gaaaaagctc atatcatcaa aaaattacaa
     3181 gaaaagcata gtgtagttgc ttttgttggt gacggaatta atgatgcacc tgccctaaaa
     3241 caagcggatg ttagtttctc tgtcagtaat gcttctgata tcgccactga tacagcagat
     3301 gtaactctct tagataacga tttaaaacta gtgttaattg ctattgattt atcaaagaaa
     3361 acaacagcca atattaaaca aaatataata tgggcatttt tatataactt agtaatgatt
     3421 ccgcttgcaa tatttaatat cgctacacca ctagtaggtg gtgtcctcca tgttatttct
     3481 agtcttctcg tcgtttttaa tgcactgcgt ctcaatttat ataagcacaa aataaaataa
     3541 tatcttcgtt cctattaatt ttgatttatt aattatcatt tttgaaaatt caaactttag
     3601 aaaatacaca aatatctctt ttgctttttc ataatatttg tataaacctt aaaatatcat
     3661 gaaaaattca atattttcgt attctatttt tatataaaaa taagttattg gttaatttat
     3721 cgac
//
LOCUS       DAOSEX010000058         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_22866_length_3719_cov_3.111081, whole genome shotgun sequence.
ACCESSION   DAOSEX010000058 DAOSEX010000000
VERSION     DAOSEX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22866_length_3719_cov_3.111081"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="PK612_01735"
     CDS             complement(<1..1827)
                     /locus_tag="PK612_01735"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOH94868.1"
                     /translation="MKTINDKTKRLHNDRVDFSKISGNFEVPNLVEIQIDSFEWFKNE
                     GIAEVFNEIFPVESYSKDIEIGFEGFRFDTPKYTYLECKDRGFTYSAPLRVTLSLGIR
                     KTGEVVKSEIFMGDIPLMTHTGTFVINGAERVIVSQIVRSPGAYMSKELDTKSGKFIY
                     NADLNPTRGTWLNFENDAKGLLSVRIDKQRKMNATLLLKALGITDYHETIELFDVFDA
                     KNRAKNNIRKSEEEMTSLELTLRKDESFTTSLEALYEIFRRLKPGEPMSDEGVKNHLI
                     QKFSDPKRYDLGKAGRFKFAKKLGIYERLEGVVLAEDLVSVDGEILFEKEHQMKKEDV
                     DKLRALKFFEKGAHTYTIPFNPDLDDYNTVNIVKAYVNDEKTQAINIVGTDLNIDEKR
                     VTTPDIVAVFSYFLNLMHGIGRVDDIDHLSNRRVRPVGELIQNQFRVGLSKMVKAMKD
                     SSSIADTETITVQKLVNTRAVASAIRDFFATSQLSQFMDQVNPLAELTNKRRISALGP
                     GGLTRDRASFDVRDVHYSHYGRICPVETPEGQNIGLINNLACYAKVNEYGFIETPYRK
                     VLKPYGQITNETIYLDADEEKEFVIAEANVEVDENGVIQTQEI"
     gene            complement(1855..2454)
                     /locus_tag="PK612_01740"
     CDS             complement(1855..2454)
                     /locus_tag="PK612_01740"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOH94869.1"
                     /translation="MSHYFTNNNELEHDIRTLELSLQGTNLTLITDRGVFSNKEIDEG
                     SIVLLKSIIKSIDVKGEVLDVGCGYGTLGLYLAARYPEIKVTMFDINERAVSLTSENA
                     KRNNLTNTHVYVDSDYQALPKHTFNLIVINPPIHAGKKAYYPLLSAARDYLTDGGILM
                     FVIRKSHGAKSAAKYLSDYYSNITLIQKDAGFYVYHAQK"
     gene            complement(2525..2899)
                     /gene="rplL"
                     /locus_tag="PK612_01745"
     CDS             complement(2525..2899)
                     /gene="rplL"
                     /locus_tag="PK612_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOH94870.1"
                     /translation="MAKLTVTDIIAALKEMTVLELNDLVKAVEEEFGVTAAVATAGPA
                     PEDDGRVDKGPSEVTLVLKHWGQSKVAVIKAVQAITGLGLMDAKKLVDGAPVNIKEKI
                     SPVEAEEHKKALVEAGADVEIK"
     gene            complement(2924..3412)
                     /gene="rplJ"
                     /locus_tag="PK612_01750"
     CDS             complement(2924..3412)
                     /gene="rplJ"
                     /locus_tag="PK612_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOH94871.1"
                     /translation="MNQKSIVAKEAIVKEVVDLAKSHEAFIVFTYHGLSVSQITELRR
                     LLRADGNTMGVYKNSLVERAVKELGHEELNDYLTGPNAFLFSKDVSSGAKALTRFARF
                     NDVVEIKGGLFEGNRVVDGAEVRNIGRLPDRNGMISMFLSVLNAPIQKFAATVKAVAE
                     KQ"
BASE COUNT         1016 a          756 c          689 g         1258 t
ORIGIN      
        1 gatttcttga gtttgaatca caccattttc atcaacttca acgtttgctt cggcaataac
       61 gaactctttt tcttcgtccg cgtctaaata aatcgtttcg tttgtaattt gaccatatgg
      121 ttttaagact ttacgatatg gtgtttcaat aaagccatat tcattaactt tcgcataaca
      181 cgctaggtta ttaataagcc caatattttg tccttctggc gtttcaactg gacaaatacg
      241 accatagtgc gaatagtgaa cgtcacgcac atcaaagctt gcgcggtcac gggtaagtcc
      301 gccaggacct aaggcactaa tacgccgttt gtttgtaagt tccgcaagtg ggttaacttg
      361 gtccataaat tgcgaaagct gacttgtcgc gaagaaatca cgaatcgcac tcgctactgc
      421 tcgagtattg acaagttttt ggacagtaat agtttctgta tcagcaattg atgaagaatc
      481 tttcattgct ttaaccattt ttgaaagtcc aacacggaat tgattttgaa ttaattcgcc
      541 aactggacga acacgtctat tgctaaggtg gtcaatatcg tcaacgcgac caataccgtg
      601 cattagattt aagaaatagg agaaaacagc gacaatatca ggagtcgtga cgcgtttttc
      661 gtcaatattt aaatcagtgc caacaatatt aattgcttgt gttttttcat cattaacata
      721 agccttaaca atatttacag tattataatc atctaaatca gggttaaaag gaatcgtata
      781 agtatgggca cctttttcga agaatttaag ggcacggagc ttatcgacat cttctttttt
      841 catttggtgt tctttttcga aaagaatttc gccgtcaaca cttactaaat cttcagcaag
      901 gacaacgcct tctaaacgtt cgtagatacc aagttttttc gcaaatttaa agcgacccgc
      961 tttgcctaag tcataacgct ttggatcaga gaatttttga ataaggtgat ttttaacccc
     1021 ttcatcactc attggttcgc ctggttttaa gcgccggaaa atttcgtaaa gtgcttcaag
     1081 tgaagttgta aaactttcgt ctttacgcaa agttaattca agcgaagtca tctcttcttc
     1141 cgatttacgt atattatttt tggcgcggtt tttcgcgtca aacacatcaa agagttcaat
     1201 tgtctcgtga taatcggtaa tgccaagtgc ctttaaaagc aacgttgcat tcattttccg
     1261 ttgtttatca atccggactg ataataaacc tttcgcgtca ttttcaaagt ttaaccatgt
     1321 cccgcgcgtt ggatttaaat cagcgttata aataaactta cctgatttag tgtctaactc
     1381 tttggacata taggcgcctg gtgaacgaac aatttgcgag acgattactc gttcagcacc
     1441 gttaataacg aatgtaccgg tatgtgtcat taaagggata tcacccataa agatttcact
     1501 tttaacgact tcacccgttt ttctaatccc aagcgataat gtaacgcgta atggtgcgct
     1561 ataagtaaag ccacgatctt tacattctaa atacgtatat tttggagtat caaagcggaa
     1621 tccttcaaag ccaatttcaa tgtctttgct ataagattca acagggaaga tttcattaaa
     1681 tacttccgcg ataccttcat ttttgaacca ctcgaatgag tcgatttgaa tttctacaag
     1741 attaggaact tcgaagtttc cgctgatctt gctaaagtca actcggtcat tgtggagtct
     1801 ttttgtttta tcgttaattg ttttcataaa tggcctcgct ttacttgtat tttgttactt
     1861 ttgtgcgtga tagacgtaga atcccgcatc tttttgtata agtgtgatat ttgaataata
     1921 gtcacttaag tactttgcgg cacttttagc gccatgggac ttacgaatca caaacatcag
     1981 tattccgcca tcagtgagat agtcacgcgc cgcactaaga agggggtaat aagctttttt
     2041 acccgcatga attggtgggt taataactat caagttgaat gtatgtttag gtagagcctg
     2101 atagtcacta tctacataaa catgtgtatt tgtgaggttg ttgcgtttag cgttctcact
     2161 cgttagactt acagcgcgtt cattaatgtc gaacattgtg actttaattt cggggtagcg
     2221 tgcggctaaa tataagccaa gcgtcccgta accacacccg acatcaagta cttcgccctt
     2281 tacgtctatg ctcttaataa tacttttgag tagtacaatg gacccttcat caatttcttt
     2341 attgctgaag actccgcgat cagtaattag cgttaaattt gtgccttgta atgataattc
     2401 taatgtgcga atatcatgtt ctagctcgtt attatttgta aaataatgac tcaaattcaa
     2461 ccttccttaa acgcacgaaa acccacaaca tgaattgtgg gtcttcgcat cttgcttatt
     2521 taagttattt aatttcaaca tccgcaccag cttcgacaag agcttttttg tgttcttctg
     2581 cttcaacagg tgagattttt tctttaatgt tgacgggggc accatcaact aattttttgg
     2641 catccattaa gcctaagcca gtgatagctt ggacagcttt aatgactgca actttacttt
     2701 gaccccagtg tttgagcact aaggtgactt ctgatggacc tttgtcaact cggccatcat
     2761 cttctggtgc tggtccagca gttgcgactg ctgcggtaac gccgaattct tcttcgactg
     2821 ctttaactaa atcatttaat tcgagtacgg tcatttcttt taatgccgca ataatgtctg
     2881 taacagttaa ttttgccatt tttcttttcc tccttgggca attttattgt ttttctgcga
     2941 ccgctttaac tgtagcggca aacttttgaa ttggtgcatt gagcactgat aagaacattg
     3001 agatcattcc gttgcgatct ggtaagcgtc caatgttacg tacttctgcg ccatcgacta
     3061 cacggtttcc ttcgaaaaga ccacctttaa tttcgacgac atcattgaag cgtgcgaagc
     3121 gtgttaatgc tttagcgcct gaactaacat ctttactaaa taagaatgcg tttggtccgg
     3181 ttaaataatc atttaattct tcgtggccaa gttctttaac tgcgcgttcg actaatgagt
     3241 ttttatatac acccatcgtg ttgccatcag cgcgtaataa gcgacgtaat tcagtaattt
     3301 gtgaaacact taagccgtgg taagtaaaca cgataaatgc ttcatgactt ttagctaaat
     3361 cgactacttc tttaacaatt gcttcctttg caacgatgct tttttgattc attgttcatc
     3421 ctccttccgt taatttggta attgaggctt caataatccc acaaggatat tcactaattt
     3481 atattctttc ctcgacaaca ttattaagct tatttccgcc gttgttttct taggttattg
     3541 atagcgtagt ttgtacttat cgaccaggga atgtaatctt gatcgatgga cccatagttg
     3601 tatggatcac agcatttttg ataaatgttc ctttaactgc agctggacga actctaacaa
     3661 tccgttcagt aagggcaaca aggttatcaa ctagtttttg gtcttcgaag cttacgcgc
//
LOCUS       DAOSEX010000059         3663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_23351_length_3663_cov_3.894124, whole genome shotgun sequence.
ACCESSION   DAOSEX010000059 DAOSEX010000000
VERSION     DAOSEX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23351_length_3663_cov_3.894124"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PK612_01755"
     CDS             <1..258
                     /locus_tag="PK612_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94872.1"
                     /translation="FLIIYVLVFKRKYIYAALMSFSSVLTYTHFLTGQYIFKFLDKDC
                     VRLSALINLGLIIALIIDAKSVLEKEPAEELLELETEQSEV"
     gene            complement(255..3005)
                     /locus_tag="PK612_01760"
     CDS             complement(255..3005)
                     /locus_tag="PK612_01760"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOH94873.1"
                     /translation="MKSLINKIKCIKKEDISRITFTALPFVLAFFIPFILAIILFSKH
                     GYYPFSADGHSLAMVDLRGQYIAFFRYYKTILDGDNTILYTLGKVTGGDFLSIFTYYL
                     ASPFNLVLKFVANVELPKALMWLVIIKIALSGLTAYISLAAINKNGFVNLIFSIAYAL
                     MAYNFVYYSNIMWLDGVLALPLVALGIHLLTQEKSALLYIFALAYVIATSWYIGIMVA
                     MFSVFFFLYNFISLNSRMYFRKKLLLTFTLSSLLAGLISFAFWGSALVNILGTKGSMS
                     FTNLSKRIKEFYALVDIERGFLYGNHIGMKDIIEPTSVIYAGALPLFLMIMFFANRAF
                     SRKQRIAALTLVLIYVVALFNKGVDHLFHGGPAPNWFPCRYGFIFSFIVIYFASLNFR
                     EYKATKWYAFIAPVIVYLFLIISLTKNEFAYDKKSILYFSITFCVLVIFFVLNEFIFK
                     QKGEEDNSKRVQKYVTTSVSFLLIIVSAINVFGNSNRILSVFSDTVYHESMTAYRNDE
                     RIGRAIDFLKKSDNSLYRLEKSFIRSGTYNNANNDSMYFNYNGISHYSSSEKKQVMDY
                     MLKIGFHYNGFNLNYANGSTLAINSYLGVKYLLDNGVNRNFDFVRELPLVDDGLNDDI
                     NTYLNTYALPFLMPIEDTNISYVGDGVYVDGGSSVYWFDMFEYQNHLFKTITRTVKDE
                     LGKPKDIFKKATYTKTLSNVTPLPRDYYYKVVSSGTIDYDITLDKNTNYYYYIHGPKS
                     NDLALYDRNIYTTYFSYHGYQINGIRRNTASTRLSVAITKPQESIKLQEAIYYEDLSV
                     LNEYINAIKAIAEVDIKQEKTSKYSASVVTKKDNQYMLLTLPYNQNLRVFIDDKKVET
                     TTRFNIFTGFTIPKAGAHKITIKYVELTYQLGIPVGILSAAGTIVISVLNKKKKEEQE
                     KA"
     gene            complement(2980..3408)
                     /locus_tag="PK612_01765"
     CDS             complement(2980..3408)
                     /locus_tag="PK612_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94874.1"
                     /translation="MKFKKKVIIKLDTINILMVLSFFLPLATLRKRLPERGLVINYVS
                     GFNMFASPLWPFILPYFIPLLIFIATSFFYFKSKKVRVINFIMLLIMIILGAIAISIF
                     YGYGTLYNIAFLICVVLMITLLVNFKKEEVIQSEITDKQN"
BASE COUNT         1337 a          590 c          568 g         1168 t
ORIGIN      
        1 tttttaatta tttatgtttt agtatttaaa cgtaagtaca tttatgcggc actgatgtct
       61 ttttcaagcg tcttaactta tacacacttt ttaactggtc aatacatctt caagttttta
      121 gataaagact gtgtaagact ttctgcgcta ataaatttag gattaattat tgctttaata
      181 attgacgcta aaagtgtatt agaaaaagaa ccggcagaag aattacttga acttgaaaca
      241 gaacaaagtg aagtttaggc tttttcttgt tcttcttttt tctttttatt taaaacactg
      301 attacgatag tgcccgctgc acttaatata ccaacaggaa taccaagttg ataagttaat
      361 tcaacatatt ttatagtgat tttatgggca ccggctttag ggatagtaaa gcctgtaaaa
      421 atattaaaac gcgttgttgt ctcaactttt ttatcgtcaa taaacacgcg taagttttga
      481 ttataaggca aagttaatag catatattga ttatcttttt tcgtcactac actagcgctg
      541 tacttagatg ttttttcttg cttaatatca acttcagcaa tagccttaat cgcattaatg
      601 tattcattta aaacacttaa gtcttcgtaa tatatcgctt cctgtaattt aatactttct
      661 tgtggtttag taattgccac acttaatcgt gtactagcgg tgttacgcct aataccgtta
      721 atttgataac cgtgataaga aaaatatgtc gtgtatatat tgcgatcata taacgctaag
      781 tcattactct ttggtccgtg aatatagtaa taatagtttg tgtttttatc aagtgtaata
      841 tcataatcaa tcgtgccgga actaactact ttatagtaat aatctcttgg gagcggagta
      901 acattactta gcgtctttgt ataagttgct tttttaaaga tgtctttagg tttacctagt
      961 tcatctttca cagtgcgtgt aattgtttta aataaatgat tttgatattc aaacatgtca
     1021 aaccaataaa cactgctacc accatcaaca tatacaccat ctccaacata actaatattc
     1081 gtatcttcaa tcggcattaa aaatggtaag gcgtaagtgt taagatatgt atttatatca
     1141 tcattaagtc cgtcatccac aagcggtaat tcacgcacaa agtcaaagtt acgattcact
     1201 ccattatcaa gtagatactt tacacctaaa tagctattaa tcgctaaagt tgaaccgttc
     1261 gcataattta aattaaagcc gttataatga aatccaatct ttagcatata atccattact
     1321 tgcttttttt cgcttgaaga atagtgtgaa ataccgttgt aattaaaata cattgagtca
     1381 ttattagcat tattatatgt accgctgcgg atgaatgatt tctctaaacg atagagtgaa
     1441 ttatcgcttt ttttaaggaa atcaattgct ctgccaatgc gttcatcatt acgatatgcc
     1501 gtcattgatt cgtggtatac tgtatcacta aacactgaca aaatgcggtt agaattgcca
     1561 aatacattaa tcgcgctcac gattataagc aggaaagaaa cactcgtcgt aacatatttt
     1621 tgtactcttt tagaattgtc ttcttcccct ttttgcttaa agataaattc attgagcacg
     1681 aagaaaataa ctaagacgca aaaggttata ctaaaatata aaatactttt tttatcatac
     1741 gcaaattcat ttttggtaag cgaaataatt aagaataaat aaacgattac cggagcaata
     1801 aaagcatacc attttgtcgc tttatactct ctaaagttaa gggaagcaaa gtaaataaca
     1861 ataaaactaa aaataaaccc gtagcggcac ggaaaccagt tcggggctgg accaccgtgg
     1921 aagagatgat caacaccttt attaaataac gctactacat aaattaacac tagagttaaa
     1981 gctgcaattc tttgtttacg actaaatgca cgattagcaa agaacataat cattaaaaat
     2041 agtggtaatg caccagcgta aattacactt gtgggttcaa taatatcttt cattccaata
     2101 tgattcccgt ataggaaacc gcgttcaata tcaacaagcg cataaaattc tttaattctt
     2161 tttgataagt tagtaaaact cattgagcct tttgtcccta aaatatttac aagcgctgaa
     2221 ccccaaaagg caaacgaaat taaacctgct agtagtgaac ttaaagtaaa ggttaaaagt
     2281 aattttttcc gaaaatacat tcgtgaattt agacttataa aattgtaaag gaaaaagaaa
     2341 acagaaaaca ttgccaccat aatcccgata taccacgaag tagcaatgac ataggctaac
     2401 gcaaaaatgt ataaaagcgc actcttctct tgtgtaagta gatgaatacc taaagcaaca
     2461 agcggtaaag ctaatactcc atcaagccac ataatgtttg aataatagac aaagttatac
     2521 gccattagtg catacgcaat actaaagatt aagttaacaa aaccgttttt attaatagcc
     2581 gctaagctta tataagcagt taaaccactt agtgctattt taataatgac aagccacatt
     2641 aaggcttttg gtaattcaac atttgcaaca aactttagca ctaaattaaa gggggatgct
     2701 aagtagtaag tgaaaattga aagaaaatca ccaccagtta ctttccctaa tgtatataaa
     2761 atagtattgt cgccatctaa tatcgtttta taataacgga aaaaggcaat atactgtccg
     2821 cgcaaatcaa ccatcgctaa agaatgcccg tcggcactaa aagggtaata tccatgtttt
     2881 gaaaataaaa taatagcaag gataaacgga ataaaaaaag ctaagacaaa aggtaaagca
     2941 gtaaatgtta tacgactgat atcttctttt ttaatacact taattttgtt tatcagtgat
     3001 ttcactttgg attacttctt ctttcttaaa gtttacaaga agcgtaatca ttaacacaac
     3061 acaaattaaa aaggcaatat tatagagtgt cccatatccg tagaatatac taatcgcaat
     3121 agcgcctagt ataatcataa tgagcaacat tataaaatta atgacccgta cttttttact
     3181 tttaaagtag aaaaatgatg tagcaataaa aataagaagc gggatgaaat aaggtaagat
     3241 aaacggccac aatggtgaag cgaacatatt aaagccgctg acataattaa tgacgagccc
     3301 gcgttcaggt aaacgttttc gtaatgttgc aagcggtaaa aagaaactta aaaccattaa
     3361 tatattaata gtatcgagtt taataattac tttcttctta aatttcatgt ttcacctctt
     3421 tgtttaatta tagtatgaaa ataataaatt gttacatgaa aaagataaag tgacaaaata
     3481 gaaaataaag ttttaaaaat agatattttc aatttagcgg atattaaagt gacaaaaaag
     3541 ggtattgtaa agtaaaaaat attgttataa tgagggtgga agaagcaaaa agagttaaaa
     3601 actttgcgcg gcgaaaagaa ccgcctcaaa gtttgcaaaa cttatattga caagttataa
     3661 ggg
//
LOCUS       DAOSEX010000060         3624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_23708_length_3624_cov_3.501261, whole genome shotgun sequence.
ACCESSION   DAOSEX010000060 DAOSEX010000000
VERSION     DAOSEX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23708_length_3624_cov_3.501261"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PK612_01770"
     CDS             <1..684
                     /locus_tag="PK612_01770"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH94875.1"
                     /translation="ADVNKITKEDDYFVIHSDEETLYAKAVIVATGTQERKLKIPGEA
                     RYTGKGVSYCAICDGNLYKNDAIMIIGAGNSAIEEALFVSKIVKHVHIVHRNERFRAD
                     ESLVNELKAMPNVTFHLNYIPVEILGDTKGVTSVAIKNVIDGTDKVIEVKAVFPFVGS
                     DASADFLSEFDVRDDKGFVLVNEEMMTSVPGLFSIGDVNQKVLRQIVTATNDGAIAAL
                     AANRFVKRK"
     gene            744..1721
                     /gene="whiA"
                     /locus_tag="PK612_01775"
     CDS             744..1721
                     /gene="whiA"
                     /locus_tag="PK612_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOH94876.1"
                     /translation="MKNVSFTTRAKEDITFNTISYSLPRRKATLAALIKHAGHLIIRE
                     KKEVLKLSTENAKVAKYIYQLFKDIFNFEPTFSYVTKRKFGKKMKFNVIVEQKVDEII
                     ETLAVDFYDSAVSRKIVYSDDTLGGYFTGAFLGSGSMTSPLSSNYHLEIVVRQKKYAE
                     HLARMLNRFEKTNFNAKVTARRDKYIVYLKRSDQISSFLVFVGAANASLEFEGIRMDR
                     DMQNITNRMFICDEANYNKTVKSATEQIENIKIVQRVYGHTPHNNEKLGALMRLRLEN
                     EDATMNELAEMLTEELGSEKRITKSNVNHLFIKLRELAEPFKELSRGNK"
     gene            1708..1929
                     /locus_tag="PK612_01780"
     CDS             1708..1929
                     /locus_tag="PK612_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH94877.1"
                     /translation="MEINEAFGIRLRQLRKAKGWSLLELSVRANVNHNYLCDLENGRR
                     NPTLALLYRISEAFGITLSSLLLGIELIQ"
     gene            2070..3101
                     /gene="gap"
                     /locus_tag="PK612_01785"
     CDS             2070..3101
                     /gene="gap"
                     /locus_tag="PK612_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOH94878.1"
                     /translation="MSIKIAINGFGRIGRLAFRRIAETKGMEVVAINDLTDAENLAYL
                     LKYDSAHGRFHVDDISVTADGTAIVFKGKEIPVYAQPDPTQLNWTKHGVDIVLECTGR
                     FRGKEDASVHLQEGVKAVIISAPADKETPMFVYGVNTDKLTKDMTVISGASCTTNALA
                     PVVKVLDDNFGVERGLMTTVHAYTNDQTILDLPKKKAARRGRAGAMNIVPTSTGAAKA
                     IGQVLPHLNKKLDGGALRVPVIDGSMVDLTVLLKKKVTAEEINAVMKKAAEGELKAIL
                     GYTEDEVVSGDIIGDTHGGLFDATLTKVMEVDGEQLVKVMFWYDNEYGYTCQYIRLAQ
                     EVAKVHGVK"
     gene            3163..>3624
                     /gene="pgk"
                     /locus_tag="PK612_01790"
     CDS             3163..>3624
                     /gene="pgk"
                     /locus_tag="PK612_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOH94879.1"
                     /translation="MAKIIRDLKLANKTVLIRCDFNVPFKGEVISDDNRIRAALPTIK
                     FALDSGAKVVLFSHLGKIKHKGTEEEVEAAKKKNNMVIVFRRLAEILGEDKVFFSPET
                     SGEEVNKRVALLKPGQVLLLQNTRYEKGEEKNDPVLSEKWASFADVFVMDAF"
BASE COUNT         1219 a          590 c          772 g         1043 t
ORIGIN      
        1 gctgatgtaa ataaaattac caaagaagac gattattttg taattcatag tgatgaagag
       61 acgctttacg caaaagctgt cattgtcgca acgggtaccc aagagcgaaa actaaaaata
      121 ccaggtgaag cgcgttacac cggtaaaggt gtaagttact gcgctatttg tgacgggaac
      181 ttatataaaa atgatgcgat tatgattatt ggcgctggta atagtgcaat tgaagaagca
      241 ctctttgtaa gtaaaatcgt taaacatgtg catattgttc atcgtaatga acgctttaga
      301 gcagatgaat cccttgttaa tgaacttaaa gcaatgccaa acgttacttt ccacttaaac
      361 tatattccag ttgaaatctt aggggacact aaaggtgtca ctagtgttgc aattaagaat
      421 gtaattgatg gcaccgacaa agttattgaa gtcaaagccg tgttcccgtt tgtgggaagt
      481 gatgcatccg cggatttctt aagtgaattt gatgtccgtg acgataaagg gtttgtgtta
      541 gtgaatgaag aaatgatgac gagtgtccct gggctcttta gtattggtga cgttaatcaa
      601 aaagtattac ggcaaattgt aaccgctaca aacgacggcg cgattgctgc tctcgcggcg
      661 aatcgttttg tgaaacgaaa ataactaatt agtaaataag tgaaatttgg gggaaattcc
      721 cccttttcct ttataatata agtatgaaaa atgtgtcatt tactacaaga gcaaaagaag
      781 atattacttt taatacaata tcctattcct tgccaagacg gaaagcaaca cttgccgcgc
      841 tcattaagca tgcaggtcat ttaataattc gtgagaagaa agaagtttta aaacttagca
      901 cggaaaacgc taaggtagcg aaatatattt atcaattatt taaagatatc tttaattttg
      961 aaccaacatt ttcgtatgtt acaaaacgta aatttgggaa aaagatgaaa tttaatgtga
     1021 ttgttgaaca aaaggttgat gaaattattg aaacacttgc ggttgatttt tatgatagcg
     1081 ctgtgagtcg gaagattgta tatagtgatg atactttagg tggatacttt acgggtgctt
     1141 ttttaggcag tggttcaatg acaagtccct taagttccaa ttaccattta gaaattgttg
     1201 tgcgccaaaa gaaatacgcc gaacacttag cgcgaatgct taaccgtttt gaaaagacaa
     1261 actttaacgc taaagtaact gcaagacgtg ataaatatat tgtgtactta aagcgaagtg
     1321 accaaatttc gtcgtttctt gtctttgtcg gagcggcgaa cgctagttta gaatttgaag
     1381 ggatcagaat ggaccgcgac atgcaaaata ttacgaaccg gatgtttata tgcgacgaag
     1441 ctaattacaa taaaacagtt aaaagtgcca ctgaacaaat tgaaaacatt aagattgtac
     1501 aaagagtgta cggacatact ccgcataata atgaaaaact cggagcactg atgcgtttac
     1561 gtttagaaaa tgaagacgcg acaatgaatg aactcgcgga aatgttaaca gaagaattag
     1621 gaagtgaaaa aagaattact aaaagtaatg ttaatcattt atttattaag ttacgggaac
     1681 tagcagaacc ctttaaggag ttaagtcgtg gaaataaatg aagcctttgg gattagatta
     1741 cggcaattac gaaaagcaaa gggctggagt cttttagaac tttcagtacg ggcgaatgtg
     1801 aatcacaatt atttatgtga tttagaaaac ggaagacgta accccacttt agcgcttctc
     1861 taccgcatta gtgaagcctt tggtattact ttatcatcct tacttttagg gattgaactc
     1921 attcaataaa actaattttg attaaaaaaa gtggaattcg ggatattttt acctaataag
     1981 tgaaaatatt cgtttttttg tatgtttttg ttagtacatt agataactaa tgttgtataa
     2041 tataaacgca taaataaagg aggaaaatta tgtcaattaa aattgcaatt aatggatttg
     2101 gccggattgg tcgcttagca ttccgtcgga ttgccgaaac aaaaggaatg gaagttgtcg
     2161 cgattaacga cttaactgat gctgaaaatt tagcatacct tttaaaatat gatagcgcac
     2221 acggtcgttt ccacgttgat gacattagcg tgaccgctga tggaacagca atcgttttta
     2281 aaggaaagga aatcccagtt tacgctcaac ctgatccaac ccaattaaat tggacaaaac
     2341 acggtgttga tatcgtctta gaatgtactg gtcgtttccg tggtaaagaa gatgcaagtg
     2401 tgcacttaca agaaggtgtg aaagccgtca tcattagtgc tcctgctgat aaagaaaccc
     2461 caatgtttgt ttacggtgtt aataccgata aattaacaaa agatatgacc gtcattagtg
     2521 gtgcaagctg taccacaaac gcactagcgc cagtcgtgaa agtgcttgat gacaattttg
     2581 gtgtcgaacg cggtttaatg actactgttc acgcttacac gaatgaccaa acaatcttag
     2641 acttaccaaa gaaaaaagct gcacgtcgtg gtcgggcagg tgcgatgaat atcgttccaa
     2701 cttcaaccgg tgccgctaaa gcaattggtc aagtactccc acatcttaac aaaaagcttg
     2761 atggtggtgc tttacgtgtc ccagtaatcg atggttcaat ggttgactta acagtgctct
     2821 taaagaaaaa agttactgct gaagaaatta atgctgtgat gaaaaaagct gctgaaggtg
     2881 aattaaaagc cattcttggt tacaccgaag acgaagtcgt cagtggcgac atcattggtg
     2941 atacccacgg tggtttattt gacgcaacct taacgaaagt tatggaagtc gatggtgaac
     3001 aactcgttaa agtaatgttc tggtatgaca atgaatatgg ctatacttgc caatacatcc
     3061 gtcttgcgca agaagttgcc aaagttcacg gcgttaaata atttattaat gatttaaaga
     3121 aagcgccttc gggcgctttt tatctaaaaa aggagaaaaa atatggctaa aattattaga
     3181 gatcttaaat tagcaaacaa aacagtgtta attcgctgtg actttaacgt gccatttaaa
     3241 ggcgaagtca tcagtgatga taaccgtatt agagctgctt tacctacaat taagtttgcg
     3301 cttgattctg gcgcaaaagt agtgcttttc tcacaccttg gtaaaattaa acataaagga
     3361 accgaagaag aagttgaagc cgctaaaaag aaaaataata tggtaattgt cttccgccgt
     3421 ctagcggaaa ttttaggtga agataaagtc ttctttagcc ctgaaactag tggcgaagaa
     3481 gtaaataagc gtgttgcttt acttaaacca ggacaagtct tactcttaca aaatacgcgt
     3541 tacgaaaaag gtgaagagaa aaatgacccc gttcttagtg aaaagtgggc aagtttcgct
     3601 gatgtcttcg tgatggacgc tttt
//
LOCUS       DAOSEX010000061         3573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_24197_length_3573_cov_2.573906, whole genome shotgun sequence.
ACCESSION   DAOSEX010000061 DAOSEX010000000
VERSION     DAOSEX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24197_length_3573_cov_2.573906"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK612_01795"
     CDS             complement(<1..516)
                     /locus_tag="PK612_01795"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOH94880.1"
                     /translation="MATTVKGTFAAKSDRGLVRLHNEDRAAVLLNQKGDLLFVVCDGL
                     GGHNKGDFASKLAIDMLMTAFANNRGFLTTNSIKRFFNKNIRAINKAIFEESILSPDM
                     NNMSTTLNAVVLTRSKLCNVNIGDSRFYLCMDDEMYLMSEDQTVVGYLVRTNQITPEE
                     ALVHPKRHILTN"
     gene            complement(500..1564)
                     /gene="rlmN"
                     /locus_tag="PK612_01800"
     CDS             complement(500..1564)
                     /gene="rlmN"
                     /locus_tag="PK612_01800"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOH94881.1"
                     /translation="MSKKLNFYDYSLPKLQDFLLNEGFKKYSATQMFEWVYLKDELDF
                     NNMSNLALVTREFMINNFTFALPTVHELQESEDGTVKLLLKLGDGSLVETVLMRYRYG
                     NSVCVSTQVGCNMGCRFCASGLLKKKRDLTPGEMLGQVLMINEYLKGRGELKLTTHVV
                     LMGTGEPFDNFENILDFIDLLNNPKSLAIGARHITVSTVGLVPKIKEFADLKLQVKLA
                     ISLHAATDEKRNQIMPINNAYPLKEVLSAVKYYESKTNKRVTYEYIMLNDFNDTLEDA
                     RALVNLVKGTNGFVNLIAYNPIAEFDYEPSTRDHVYKFANYLKSMGVQTTVRKEFGSD
                     IDAACGQLRAKVEKKRGNNS"
     gene            complement(1548..2765)
                     /locus_tag="PK612_01805"
     CDS             complement(1548..2765)
                     /locus_tag="PK612_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94882.1"
                     /translation="MIKHILDTTEKIIDGSLPYQQFSKDLKQDNSYSQSEKHQFRTLV
                     NALLNRYFRYLYSLTEGEETTLTQFEVLALSLFSFNREKKVVPLSYEEFLAALPTDIQ
                     VKFEQEYPDESFPIRIPKHFHSENPALSLAARFSVPLALTEQLIADIGRKNLLKFLTN
                     RYRETYGLINQNVISDEDFFANMPLFIRGLKPGEYEYKGKEYIKKTPAYNNNEIVIAS
                     QTLLDVVDIIESKKPKNLLFVQHEDTSLMLVLALKYPEIQIHFYKDDNRSKYIVRHLV
                     RKFSLENVTYVDKITQTYDFVLTTLQSSNINGDIRHRDFYFRLPDNLQFFAAKGREEL
                     EELKDYVNEDGHLMFITTTVLRFETHLQAVGFVRDYPEFELILEKQHFHFTPNNETLY
                     YALLKKGAHVEEA"
     gene            complement(2826..3263)
                     /locus_tag="PK612_01810"
     CDS             complement(2826..3263)
                     /locus_tag="PK612_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94883.1"
                     /translation="MVIIKIYSQDIYVAAEISVKYADAIAKTLGLTKEDVFLLGSDGA
                     LIAEGVDQVSWFTYLEVTIPANLKHKQDALSKTLLEIFNNYGVHLMIKYDYVTKDNIV
                     KILNPDFKVFNETMVEVLPTGNYYDEDDVDWDDDWDEDEDKDD"
     gene            complement(3278..>3573)
                     /locus_tag="PK612_01815"
     CDS             complement(3278..>3573)
                     /locus_tag="PK612_01815"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94884.1"
                     /translation="PPFYFLLSITMSHQNERTVKDTILTLKLNLIKALSEDATVIGPT
                     ATFYPASGKTFNETLIVRYKNYFKIKPALLTTLSPFREQSSLYVHINVDPYDV"
BASE COUNT         1150 a          635 c          558 g         1230 t
ORIGIN      
        1 atttgttaag atgtgacgtt tggggtgcac tagtgcttct tcgggggtaa tttgatttgt
       61 gcgcactaaa taaccgacaa ctgtttggtc ttcactcatt aaatacattt catcatccat
      121 acataaataa aaacgactat caccaatatt aacattgcac aattttgacc gcgttaatac
      181 tacagcattt aatgttgttg acatattgtt catatctggg gataaaatac tttcttcaaa
      241 gatggcttta ttaatagcgc gaatattttt attaaagaaa cgtttgatgc tatttgtcgt
      301 taaaaaacca cggttattag caaaagcagt catcagcatg tcaatcgcta atttggatgc
      361 aaagtcacct ttattatgac cgccaagacc atcacaaaca acaaagagta aatcaccctt
      421 ttggtttaaa aggactgcgg cacgatcttc attgtgaagt ctaactaagc cacgatcact
      481 tttagcagcg aatgttcctt taactgttgt tgccacgttt cttctccact tttgcccgta
      541 attgtccgca ggccgcatct atatcactac caaactcttt acgtacagtt gtttgcactc
      601 ccatgctttt tagataatta gcaaatttat aaacatgatc acgagttgat ggttcataat
      661 caaattccgc aattgggtta tacgcgatta aattaacaaa accatttgtg cctttaacta
      721 ggttaactaa ggctcttgcg tcttccaaag tatcattaaa atcattaagc ataatatatt
      781 cataagtcac gcgcttatta gttttacttt catagtattt cacggcagaa agtacttctt
      841 ttaaaggata agcattgtta attggcataa tttgattccg cttttcatca gtagccgcat
      901 gtaaagaaat ggctaacttt acttgtaatt ttaaatcggc gaattcttta attttaggga
      961 ctaaacccac cgttgaaact gtaatatgac gagcaccaat cgcaagtgat tttgggttat
     1021 ttaataagtc aataaaatct aaaatatttt caaagttatc aaacggctcc ccagtcccca
     1081 taagtacgac atgcgttgta agttttaatt caccacgacc ttttagatat tcattaatca
     1141 ttaatacttg accaagcatt tcaccaggtg ttaagtcgcg ctttttcttt aaaagccctg
     1201 acgcacaaaa gcgacacccc atattgcatc cgacttgcgt tgaaacacag acgctattgc
     1261 cataacgata gcgcatcaaa actgtttcga cgagtgatcc atcaccaagc tttaaaagaa
     1321 gtttaacagt accgtcttca ctttcttgta gttcatgaac tgttggtaat gcaaaagtaa
     1381 aattattaat cataaattcg cgtgtaacta acgctaaatt actcatatta ttaaaatcta
     1441 attcgtcttt taaatagacc cactcaaaca tttgagttgc actatatttt ttaaacccct
     1501 catttaataa aaagtcttgg agtttaggta aagaatagtc ataaaaatta agcttcttcg
     1561 acatgggcac ctttctttaa aagggcataa tagagcgttt cattattagg tgtaaagtgg
     1621 aaatgttgtt tttcaagtat taattcaaac tctggataat cacgcacaaa acccactgct
     1681 tgcaagtgag tttcaaaccg caatacggta gtcgtgataa acattaaatg tccatcttca
     1741 tttacataat cttttagctc ttctaactct tcccggccct ttgcagcaaa gaattgtaag
     1801 ttgtctggta accggaagta aaaatcacga tggcgaatat caccattaat atttgaactt
     1861 tgcagtgttg tgagcacaaa gtcataagtt tgggtaattt tatctacata agtaacattt
     1921 tctaatgaaa acttccgtac taaatggcgc acgatatatt ttgagcgatt atcatcttta
     1981 taaaaatgaa tttgaatttc aggatattta agcgcaagta caagcattaa ggatgtatct
     2041 tcgtgttgca caaataacaa atttttaggt tttttgcttt cgataatatc gacaacatct
     2101 aataatgttt gactagcaat tacaatttca ttgttattgt acgctggtgt tttcttgata
     2161 tactctttcc ctttatattc atattcacca ggttttaagc cacgaataaa aagtggcata
     2221 ttagcaaaga aatcttcatc actaattaca ttttgattaa taagaccata tgtttcacga
     2281 tatctatttg taaggaattt aaggaggttt ttccgtccta tatctgcaat taattgttcg
     2341 gtaagtgcta atggtacact aaagcgagcg gctaaagaaa gcgctgggtt ttcactatga
     2401 aaatgttttg gaattcgaat tggaaaactt tcatcaggat attcttgttc aaatttaact
     2461 tgtatatcag taggaagcgc agcaagaaac tcttcataag ataaaggcac aacttttttc
     2521 tcacgattaa aactaaagag acttaatgct aaaacttcaa attgagttag agttgtttct
     2581 tcgccttcag ttaaagaata caaataacgg aagtatctgt ttaaaagagc attaactaaa
     2641 gtacgaaact gatgcttctc actttgtgag taactattat cttgctttaa gtccttacta
     2701 aattgttgat aagggagact cccatcaatg attttttctg ttgtgtctaa aatgtgtttt
     2761 atcatatgta cctacctata ttagtatatc actcttttta aaggtgtttc taattaatta
     2821 gaaaattagt cgtctttgtc ttcgtcttcg tcccaatcat catcccagtc gacatcatct
     2881 tcatcgtagt aattgccagt aggcaaaact tcaaccattg tttcattaaa aactttaaaa
     2941 tcagggttta aaattttaac aatattatct tttgttacat agtcatattt aatcataagg
     3001 tggacaccat aattattgaa tatttctaaa agcgttttgc ttaatgcatc ttgtttgtgc
     3061 tttaaatttg caggaatagt aacttctaaa tatgtaaacc aacttacttg atcaacacct
     3121 tccgcgatta aagcaccatc actaccgagt aagaatacat cttcttttgt taaacctagt
     3181 gttttagcaa tagcatcagc gtatttaaca ctgatttcag cagcgacata aatgtcttga
     3241 gaataaattt taataatgac cataatattt ttcctttcta tacatcatat ggatcaacat
     3301 ttatatgtac ataaagagaa gattgttctc taaatggact taatgtagtt aaaagtgctg
     3361 gtttaatttt aaaataattt ttataccgca caataagtgt ttcgttaaag gttttaccac
     3421 ttgctgggta aaaagtagcg gttggaccaa tcacagtcgc atcttcactc agtgctttaa
     3481 taagatttag ttttaatgtt aatatagtat cttttaccgt tctttcgttt tgatggctca
     3541 tcgtaattga taataaaaaa taaaaaggcg gat
//
LOCUS       DAOSEX010000062         3508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_24849_length_3508_cov_3.139589, whole genome shotgun sequence.
ACCESSION   DAOSEX010000062 DAOSEX010000000
VERSION     DAOSEX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24849_length_3508_cov_3.139589"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PK612_01820"
     CDS             complement(<1..968)
                     /locus_tag="PK612_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOH94885.1"
                     /translation="MRTFNYSKYKEYKWDAEILRKVSSIYKNKGKLEVLEPRNPELFE
                     KLIENAQFESVVASNAIEGIKTTNHRFKQLFKQKITPKNRSEEEIAGYKDTINVINES
                     YEHISITPNYILQLHKIMYEKVSNNSIGGRFKNVQNYISATSETGEEFTIFTPLSPVE
                     TPQAITDICNEYNKMIKEGVVNPLILTFIFLLDFLAIHPFNDGNGRMSRLLTNLLLYR
                     LSYYVGKYVSLENKIAETKDSYYDALLDAQKGWEDDNNDPTPFISYMLGIVERAYNDL
                     ETKINLLNQNLSAYDIVKQSLTSKIGKITKAEVLTLCPTLSSSSVE"
     gene            complement(1105..2514)
                     /locus_tag="PK612_01825"
     CDS             complement(1105..2514)
                     /locus_tag="PK612_01825"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOH94886.1"
                     /translation="MIKITYDNKFTTNLVLVLDETPKTKELELMLEQKLFNAKVGEVF
                     VSVNLNREGNIYLGLEKGLAEDHEKIRTAGYTLAQKLVSEKISKINLDCNEVDPKLIS
                     TLVQGLLFSNYNFDKYKSTKKEDFNVEVSLLNAPTSLKAKIKELEGVIRGVNVARDLV
                     NTRAIDLYPETYATKIVELFKGTAVEVEVFDKKEIEKLGMHAFLAVNAGSDKEPRFVV
                     MKYFGNKTSDKHITFVGKGLTYDSGGYDIKPGKSMFEMFTDMAGSAAVIGAIKAIADN
                     NLNANVVAVTALTENLINGKAYKNGDIISTMKGSTVEIGSTDAEGRLTLADAIYYAAT
                     KLNSTHIIELSTLTGACVVALGTDMIGATTNNEEFYNKVFDAGNHVGEPSWRLPVTDV
                     LKEAVKGEFADLKNSVPGGAGTITAGIFLEHFSENIPFVHLDIAGTAYGKAKRYYANG
                     ATGVGVRTLYKLVATNFSK"
     gene            complement(2687..3193)
                     /locus_tag="PK612_01830"
     CDS             complement(2687..3193)
                     /locus_tag="PK612_01830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOH94887.1"
                     /translation="MELRDKLRTDPLTLVANRNFFDFQINKRLEEARLFGNKFGILMM
                     DIDHFKKVNDTYGHLVGDEIFKIVAYTLSSNVKKTDMVSRWGGEEFIALVDVTTNEEL
                     AAIAERLRTLVAASGYKLNVDKTIQVTISIGGTLVTKDDTIKSAITRADDNMYYAKQN
                     GRNQTKIA"
     gene            complement(3184..>3508)
                     /locus_tag="PK612_01835"
     CDS             complement(3184..>3508)
                     /locus_tag="PK612_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94888.1"
                     /translation="TFGDNVTFKKITDTIEEMVNSYCYQNILQHVDEKGTLLCIEGCP
                     LNATLQDGKIREARVFLKHKDGYRIPVMVKTLPIYDIDKRLSRRLKFLLMNASKNKFI
                     TKILN"
BASE COUNT         1046 a          663 c          513 g         1286 t
ORIGIN      
        1 ttttcaactg aacttgatga taatgttggg cataaagtta gtacttctgc cttcgttatt
       61 ttccctattt tagaagttaa actttgttta acaatgtcat aggctgaaag gttttgattt
      121 agtaaattaa ttttagtttc taaatcatta tatgctcttt caactatgcc tagcatataa
      181 ctaataaatg gagtgggatc attgttatca tcttcccagc ccttttgtgc atcaagcaat
      241 gcatcataat atgaatcttt tgtttccgct attttgtttt caagtgatac atatttgcct
      301 acataataac taaggcgata taaaagaaga ttagtcagta aacgactcat ccgaccattc
      361 ccgtcattaa aagggtggat agctaggaaa tcaagtaaaa agataaaggt aagtattaac
      421 gggttaacta cgccttcttt aatcatttta ttatattcgt tacaaatatc tgtaattgct
      481 tgtggggttt ctactggtga taagggcgta aatattgtaa actcttcacc tgtttcacta
      541 gtcgcactaa tgtaattttg aacattttta aagcgcccgc caatagagtt attactaact
      601 ttttcataca tgattttgtg tagttgcaaa atataattag gagtgatact aatgtgttca
      661 taactttcat taataacatt aattgtatct ttatatccag caatttcttc ttcacttcga
      721 tttttaggcg ttattttttg tttaaataat tgcttaaacc gatgattcgt ggtcttaatg
      781 ccttcaatgg cattactcgc cacaacactt tcaaattgag cgttttctat taatttctca
      841 aataattcag gatttcgagg ttctaaaact tctaacttgc ctttattttt atagatactt
      901 gaaacttttc ttaaaatttc agcatcccat ttgtattctt tgtattttga ataattgaat
      961 gttcgcataa tattcacctt tctcataaat tataacacct tttacgagaa taacaacact
     1021 tttacgagaa aaagatattt tacaaaaata ccccattcct aatttctagg atttggagta
     1081 tgtataataa gcaatattaa tttattattt agagaaattc gtcgctacaa gtttatataa
     1141 agttctaacg cccactcctg ttgccccgtt cgcgtaatac cttttagctt taccatacgc
     1201 cgtccccgca atatctaaat ggacaaatgg aatattttca ctgaagtgtt caaggaatat
     1261 accagcggta atcgtgcccg ctccaccagg aacagagttt ttaagatcgg caaattcacc
     1321 ttttactgct tcttttaaga cgtcagtaac aggtaagcgc caactaggtt caccgacatg
     1381 attaccagca tcaaatactt tattgtaaaa ttcttcatta ttagtggtcg caccaatcat
     1441 atccgtacca agcgctacaa cacaagcacc agttaatgtt gatagttcaa taatgtgcgt
     1501 ggaatttaat ttagtggcgg cgtaataaat agcgtccgca agtgtaagtc ttccttcagc
     1561 gtcagttgaa ccgatttcaa cagtggaacc tttcatcgtt gaaataatgt ccccattttt
     1621 atatgcttta ccattaatta agttttcggt taaagctgta actgcgacaa catttgcgtt
     1681 taagttatta tcggcaattg ctttaattgc gccaataact gccgctgaac ccgccatatc
     1741 agtaaacatt tcaaacatac ttttacccgg tttaatatcg taaccgcctg agtcataagt
     1801 taagccttta ccaacaaagg taatatgttt atcactcgtt ttattgccaa aatatttcat
     1861 gaccacaaaa cgcggttctt tatcacttcc ggcattaaca gcaagaaaag catgcattcc
     1921 taatttttca atttcttttt tatcaaagac ttctacttca acagcagtgc ctttaaataa
     1981 ttccacaatc ttagtagcat atgtttctgg atagagatca atagcgcgcg tatttactaa
     2041 gtcacgcgca acattaacac ctctaattac tccttctaat tctttaattt tagcttttaa
     2101 acttgtagga gcattaagca aagaaacttc tacattaaag tcttctttct tagttgattt
     2161 atatttatca aaattataat tactaaataa taagccttgc actaaagtcg aaatgagttt
     2221 tggatcaact tcattacaat ctagattaat cttactaatc ttttcactca ctaatttttg
     2281 cgctagagta tagccagcag ttcttatttt ttcatggtct tccgctaagc ctttttcaag
     2341 tccaaggtaa atattacctt cacgatttaa attaacacta acaaaaactt cgcccacttt
     2401 ggcattaaat agcttttgct caagcattaa ttcaagttct tttgtttttg gtgtttcgtc
     2461 taaaactaac actaagtttg tcgtaaattt attgtcataa gttattttaa tcattttctt
     2521 ttcctccact tcacaatgta gcatatttat catttgtctt tgtttaaaat gcgtccgatt
     2581 tagtcataac tggggttaat ttcctaaatg ttttattaca aaaagggagt tcaattccct
     2641 ttttgtcatt ttgctgttgt taaaaaatat ctatattttt acttatttaa gcaatctttg
     2701 tttgattacg cccgttttgt tttgcgtagt acatgttatc atccgctcgt gtgattgcgc
     2761 tttttatcgt atcgtcttta gtcactagtg tgccaccaat tgagattgtg acttgaattg
     2821 ttttatcaac gttaagctta tatccagaag ccgcgactag tgtgcgtaat ctttccgcga
     2881 tagcggctaa ttcttcatta gtggtaacat cgacaagcgc aataaattct tcgccacccc
     2941 atctacttac catatctgtt ttcttcacgt ttgaagaaag ggtataggcg acaattttaa
     3001 aaatttcgtc acccactaag tggccatatg tatcattaac ttttttaaag tgatcaatat
     3061 ccatcattaa tatcccaaat ttattcccaa ataaacgtgc ttcttctaat cttttattaa
     3121 tttggaaatc aaagaaattc cgattagcga caagtgtaag cggatcagta cggagcttgt
     3181 ctcttaattc aatattttcg ttataaattt gtttttggaa gcgttcatca gtaaaaactt
     3241 caatcgccgc gacaatcttt tgtctatatc atatataggc aacgttttaa ccatcaccgg
     3301 gatacgatag ccatctttat gttttaaaaa gacacgggct tctcttattt tcccgtcttg
     3361 cagtgtcgcg ttaagcggac agccttcaat acataaaagc gtgccttttt catcgacatg
     3421 ttgcaaaatg ttttgataac aataactatt aaccatttct tctatagtat cagttatttt
     3481 tttaaatgta acattatctc caaatgtt
//
LOCUS       DAOSEX010000063         3462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_25318_length_3462_cov_3.389492, whole genome shotgun sequence.
ACCESSION   DAOSEX010000063 DAOSEX010000000
VERSION     DAOSEX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25318_length_3462_cov_3.389492"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..214)
                     /locus_tag="PK612_01840"
     tRNA            complement(128..214)
                     /locus_tag="PK612_01840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Leu,seq:caa)
     gene            complement(343..419)
                     /locus_tag="PK612_01845"
     tRNA            complement(343..419)
                     /locus_tag="PK612_01845"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(383..385),aa:Pro,seq:cgg)
     gene            533..1441
                     /locus_tag="PK612_01850"
     CDS             533..1441
                     /locus_tag="PK612_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94889.1"
                     /translation="MNKESGYRKTIISEYLNNLLHALNAYEGYSDGFRKLNGDFINYL
                     QARWVDLNIEVGDAIRVKFQRDRDYLSHPFLGIVIRLLEGARIHVPQREETLALLKKH
                     TNYPLHNFITFAFNFNRNDKDAPFEILSDDFIKIIFYLTNKKGYPHLENFDSFLEYKL
                     RISGVSIINLVYARRSALVEKNIDKVTEHLSLAAIQSGQDHRKFLGQKYYELATLFKK
                     HNDFAGKLLQKAVDFKNYDALLPLVKYYEEASDDKDDYTAIAFYYLVIAHHLEIPGAG
                     EKLKTYYEKGGVRPPCNKMLSKFFED"
     gene            complement(1443..>3462)
                     /locus_tag="PK612_01855"
     CDS             complement(1443..>3462)
                     /locus_tag="PK612_01855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF001688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOH94890.1"
                     /translation="EEMRKFGVFTEEELADMIINNPHRINDLVENITPFTKELYKPSI
                     ENVDELLENLVFETAYEIYGNPLPTLISDRIHRELTGIINNGYAVIYYLAHLIVKKAN
                     DEGFIVGSRGSVGSSVVAYFAKVSEVNPLPPHYFCPHCKFSNFRNEQRVLSGYDLPPA
                     KCPECGHDLVGEGQNIPFETFLGFEADKIPDIDLNFPEDFQKEAHNFTKELVGENNAF
                     RAGTIQTVAERTAFGHVLNYYEKQGIYRDELNNENVAYLAYHIAGVKRTTGQHPGGIV
                     VIPSEYEVYDFTPVQYPAGNTESDWLTTHFDYDSIHDNLLKLDLLGHKDPVTLKMLCD
                     LTGIKFEDVPTNDRATISLFSSHEALNLKSNPLGEVTGALGLPEFGTNFVRQLLTEAK
                     PKTFNDLVIVSGLSHGTGVWRFNADELIRSEQATLGDVIGCRDDIMTFLIRQNLHEKD
                     AFNIMESVRRGRGVEEGLETKMREAGVPHYYIESCKKIEYLFPRAHAAAYVIMAVKVA
                     WFKLNYPLEYYATFFTIRGDNFDLKTMISSEEVILKELQKFEEQRKTSELNPRDSNIV
                     ENLQLTIEMLNRGFKISNIDLYKSEATRFKVDHENNQIIPPFIVIRALGEGTAESVVE
                     ARKNGEFISIEDLVERTRLNTSNIENLKELGALEGLPESNQISLFDFM"
BASE COUNT         1117 a          661 c          567 g         1117 t
ORIGIN      
        1 ataaatgatc aataactaca aagaacataa agtacaagaa aatggtatca attcagatat
       61 taagtcaatt atcgtgtttt agccaagaaa aaaggtttga aattgtatca aaccttacgt
      121 ttcaatatgg tgcctccgat cggacttgaa ccgatacagt atctctacta atggattttg
      181 agtccatcgc gtctaccact tccaccacgg aggcacaaga taattataaa acattaacaa
      241 cataaatttt tttataaagc taaattcatt aaaaccttcg ctgagaaatt atgtgattag
      301 tcacactttt tttcaatgaa catcatttct ttaaaataaa aatggtcgga atgacaggat
      361 tcgaacctgc gaccttctgg tcccgaacca gacgcactac caagctgtgc tacatcccga
      421 ctgcacacat attatacgct gtttttaaaa attaccaaac ttttgtgtca taaacactgt
      481 tgattttatc tatacacgca atgataacct taaaatttga taaaatattt atatgaataa
      541 agaatcaggg tatcggaaaa ctataataag cgagtattta aataatcttt tacatgcact
      601 taatgcctat gaaggatata gtgatggttt ccgtaaacta aacggtgatt ttattaatta
      661 tttacaagct cgctgggtcg atctaaatat cgaagtcggt gatgctattc gggttaagtt
      721 tcagcgtgac cgtgattatt tatcgcatcc ctttttaggt attgttattc gtttacttga
      781 aggagctagg attcatgtcc cgcagcgcga agaaacgcta gcgttactaa aaaaacacac
      841 gaattatccg cttcataatt ttattacctt tgcctttaat tttaatcgca atgacaaaga
      901 tgctcctttt gaaatattaa gcgatgattt tattaagatt atcttttact taacaaataa
      961 aaaaggttat ccgcatttag aaaactttga tagtttctta gaatataaat tacgtattag
     1021 cggggttagt ataattaacc tcgtatatgc tagacgcagt gcgcttgtcg aaaaaaatat
     1081 cgacaaagta acagaacact tatccctagc agcaatacag tcaggtcaag accaccgtaa
     1141 attcttaggt caaaagtact atgaactagc gacattattt aaaaaacaca acgattttgc
     1201 cggaaaattg ttgcaaaagg ccgttgactt taaaaattat gatgcactat taccacttgt
     1261 taaatattat gaagaagcaa gtgacgataa ggatgactat accgctattg cattttatta
     1321 tcttgtaatt gctcatcacc ttgaaatacc tggtgccgga gaaaaattaa agacttacta
     1381 tgaaaaagga ggcgtaaggc ctccgtgtaa caagatgtta agtaaatttt ttgaagacta
     1441 atttacataa agtcaaataa gctaatttga ttactttctg ggagaccctc tagcgctcct
     1501 agttctttta agttttcaat gtttgaagtg tttaatcgtg ttctttctac taaatcttca
     1561 atcgaaataa attcaccatt cttccgtgct tcaaccacac tttcagcagt gccttcgcca
     1621 agtgctctaa tgacgataaa gggcggaatg atttgattat tttcatgatc aactttaaac
     1681 cgagtagcct cactcttata tagatcaata ttcgaaattt taaagccacg attaagcatc
     1741 tcaattgtta actgtaagtt ttcaacaata ttactgtcgc gcggatttaa ttccgatgtt
     1801 ttacgctgtt cctcaaattt ttgcaactct tttaagataa cttcttcgct acttatcatt
     1861 gttttaagat caaagttatc accgcggatt gtaaagaatg ttgcataata ctcaagcggg
     1921 taattaagtt taaaccaagc gactttcacc gccataataa cataagctgc agcgtgcgcc
     1981 cgtgggaaga gatattcaat tttcttacat gattcaatgt aatagtgcgg aacaccagct
     2041 tcacgcatct tcgtttcaag accctcttca acaccgcgtc cccttcgcac tgattccatg
     2101 atgttaaagg catctttttc atgtaaattt tggcgaatta aaaatgtcat aatatcatca
     2161 cggcacccaa taacatctcc aagtgttgct tgctctgacc taattaattc atcagcgtta
     2221 aaacgccaca ctccagttcc gtggcttaaa cctgaaacaa tcacaagatc attaaatgtt
     2281 tttggttttg cttccgttaa taattgacga acaaagttag tgccaaattc aggtaaacct
     2341 aaagcacctg ttacttcgcc aagcggatta ctttttaagt taagtgcttc atgacttgag
     2401 aataacgaaa ttgttgcacg gtcattagtg ggcacatctt caaacttaat accggttaaa
     2461 tcacaaagca tcttaagtgt tactggatct ttgtgaccta ataaatctag ttttaataag
     2521 ttatcatgaa ttgaatcata gtcaaagtgc gttgttaacc agtcgctttc cgtgttcccc
     2581 gctggatatt gtacgggtgt aaagtcatat acttcatatt cacttggaat tacaacaatc
     2641 ccacccgggt gttgccctgt tgtacgttta acaccagcga tgtgataagc aagataagca
     2701 acattttcat tatttaattc atcacgatat atcccttgtt tttcgtaata atttaagaca
     2761 tgaccaaacg ctgtccgttc ggccacagtt tgaatcgttc ctgctctaaa agcattattt
     2821 tcaccaacaa gttctttagt gaagttatgt gcttcttttt gaaagtcttc agggaagtta
     2881 aggtcaatat ccggaatttt atcagcttca aaaccaagaa atgtttcaaa aggaatattt
     2941 tgcccttcac caactaaatc atgtccgcat tcaggacatt tagcaggtgg taagtcataa
     3001 cccgagagta ctctttgttc attacggaaa ttactaaatt tacaatgagg gcagaagtaa
     3061 tgcggtggga gcggattaac ttctgagact ttagcgaaat aagcaacgac gcttgagcca
     3121 acactaccgc ggctaccaac aataaatcct tcgtcatttg cctttttaac aattagatgc
     3181 gctaagtagt aaataactgc atagccatta tttataatcc ctgttaattc acgatgaata
     3241 cggtcactaa ttaaagttgg taatggatta ccgtaaattt cataagctgt ttcaaacact
     3301 aagttttcta aaagttcatc aacattttca attgaaggtt tatataattc ttttgtaaat
     3361 ggcgtaatat tttccactaa atcattaatc cggtgcggat tattaataat catatctgct
     3421 aattcttctt cggtaaatac accaaactta cgcatttctt ca
//
LOCUS       DAOSEX010000064         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_25343_length_3460_cov_4.024670, whole genome shotgun sequence.
ACCESSION   DAOSEX010000064 DAOSEX010000000
VERSION     DAOSEX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25343_length_3460_cov_4.024670"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..555
                     /locus_tag="PK612_01860"
     CDS             178..555
                     /locus_tag="PK612_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HOH94891.1"
                     /translation="MTSNEKVIQLKKSGMSYRAISEKTGLPVGTIKSIWSRANKLETE
                     VSKCKECGAEIIIIKGKKPRQFCSDKCRMKWWNAHRFLVSNKSKRHYTCKYCGKEFTS
                     KDYKERKYCSHQCYIEGRYGDEN"
     gene            545..724
                     /locus_tag="PK612_01865"
     CDS             545..724
                     /locus_tag="PK612_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94892.1"
                     /translation="MKINTYSENITQYQLTMATAKKLHKLNIINDDDFSLIEEETAKK
                     YCIKKDSLYRINNLI"
     gene            760..2094
                     /locus_tag="PK612_01870"
     CDS             760..2094
                     /locus_tag="PK612_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOH94893.1"
                     /translation="MSKNDTKIKRVRHRSTLPRKKKVAAYARVSTGKEAMLHSLSAQV
                     SYYSNLIQNKRGWLYMGVYVDEAMTGTSKERPSFLKMMNDARAGKLDMIITKSISRFA
                     RNTVTLLETIRELKALNVDVYFEEQRIHSLSADGELVLTFLASYAQEESRSASENLKW
                     SIRNRFKKGLAWNTYVYGYEYENGTFIKIPKEAEIVRHIFASYLDGKGTPAIAKDLNK
                     RGILTKLNKPWRHGAIAKVLRNYLYTGNMLLQTTYKDNHLTKRKVINNGVLPKYHVEN
                     SHEPIIDIDTFNKVQHLIAEKQKNTKVRTARDEKTLYRGMLVCDKCGKDYNRRITKNK
                     AYWICSTYNFYGKESCPSKQIPEDILNEIVKSTLNLSSLNYETVNERLTNIIVQDKQV
                     KLSLKTGQEITVPWAYQSRSASWTPEMREKARQKAMKGRNKNEKDNSNTTNN"
     gene            2060..>3460
                     /locus_tag="PK612_01875"
     CDS             2060..>3460
                     /locus_tag="PK612_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOH94894.1"
                     /translation="MKKITVIPQTINPLTNNPFNNVERRKVAAYARVSTDSDEQMTSL
                     DAQIDFYTNYIKANPEWEFVKVYYDEGISGTNTKNREGFKQMIQDAINGKIDLIITKS
                     ISRFARNTVDTLVAIRKLKEKCVECFFEKENIYTFDSKGELLITIMSSIAQEESRSLS
                     QNVTWGIRKAFSDGKVRIAYKNFLGYRRGTDGRPEVIPEEAEAIRKIYRMFLNGYSIN
                     EIVYALEEDNIDSPSGSKTWRIQTVQSILRNEKYKGDALLQKTFTVDYLEKRTKVNEG
                     EVPQYYVENSHEAIIEPEEWEMVQEEIKRRAKLGKQFRGSKVFASRLVCADCGAFYGP
                     KVWHSTDKYRSEVWQCNKRFENRKLGVKCDTPYLVEDHIKKSFIKAFNLLSKDKKKVI
                     NHCKEFIDILDNTEELNRLIDIQTTEVEVLANMAKVLVEENATTAIDQADYRTRYEAL
                     EKRYQEEQANLDNLLSE"
BASE COUNT         1248 a          578 c          730 g          904 t
ORIGIN      
        1 attcgtatct ataagggatg cggtgtgttt ggtaccttgg gcacattaaa aggaagagta
       61 cagtaagcca ctactacaag atggcagcct ccgtgggtta tcctgcggag gttttttttt
      121 gttattttac tactcaaaac gtcactattt ctccttttat aagtaggagg tttaacaatg
      181 acaagtaacg agaaagtgat ccaattaaaa aagtcaggaa tgtcttaccg cgcaataagt
      241 gaaaagacag ggttacctgt tggaacaata aaatcaattt ggtcacgtgc aaataaactt
      301 gaaactgaag tgtcaaagtg taaagagtgc ggtgcggaaa taatcatcat taaagggaag
      361 aagccaagac aattttgttc cgataaatgt cgtatgaaat ggtggaatgc tcatcgcttt
      421 ttagtaagca ataaatctaa gcgccactac acttgtaaat attgtggcaa agagtttaca
      481 tcaaaggatt ataaagaaag aaaatactgc tcgcatcagt gttacataga agggagatat
      541 ggcgatgaaa attaatactt attctgaaaa tattacgcag taccagctaa cgatggcaac
      601 tgcaaaaaaa ctccataaat tgaatattat caatgatgac gacttcagtt taattgagga
      661 agaaacagca aaaaaatatt gtatcaaaaa agatagtctt tatcgcatta ataacttgat
      721 ataaaccctc tttttagtaa atatggtagt ggctagatta tgagtaaaaa tgatacaaag
      781 attaagcgtg taagacatcg aagcacacta ccacgtaaaa agaaagtagc ggcttacgct
      841 cgagtttcta ctggtaaaga agcaatgctt cattcactat ccgcccaagt aagttattat
      901 agcaatttaa ttcaaaataa gcgtgggtgg ctttatatgg gtgtttatgt cgatgaagca
      961 atgactggca caagtaaaga acgcccgagt ttcttaaaaa tgatgaatga cgctcgtgcg
     1021 ggtaaattag atatgattat cactaaatcg atctcccgtt ttgcgagaaa cacggtgacg
     1081 ttactagaaa caattcgtga gttaaaagcg ttaaatgtag atgtctattt tgaagagcaa
     1141 agaattcatt cccttagtgc ggacggcgaa ttagtactta ccttccttgc ttcatatgcc
     1201 caagaagaat cgagatccgc tagcgagaat cttaaatgga gtattagaaa tagattcaaa
     1261 aaggggctag catggaatac ctatgtttat ggatatgaat atgaaaacgg aacattcatt
     1321 aagattccaa aagaagccga gatagtaagg catattttcg catcttacct tgatgggaaa
     1381 ggcacacctg cgatcgcaaa agatctgaac aagcgcggga ttttaacaaa gttaaacaag
     1441 ccttggcggc atggtgcaat tgcaaaggtg ctgcgtaact atctctacac cggaaacatg
     1501 ctacttcaaa caacatataa agataatcat ttaacgaaac ggaaagttat aaataacggt
     1561 gttttgccga aatatcatgt tgaaaactcg catgaaccga ttatcgatat cgacacattt
     1621 aataaagtgc agcatctcat agcggaaaag caaaagaaca ccaaagttag aaccgctcga
     1681 gacgaaaaga cgctttatcg aggaatgctt gtatgtgata agtgtggcaa agactataat
     1741 cgacgcataa cgaaaaataa ggcttattgg atatgctcga catataactt ttatggcaaa
     1801 gaatcttgtc cctctaaaca aattcccgaa gatattttaa acgaaattgt taagagtact
     1861 ttaaatctta gttcacttaa ttatgaaacg gttaatgaac gattaactaa tattatcgtt
     1921 caagataagc aagttaaact tagtttaaaa acgggacaag aaattactgt tccatgggca
     1981 tatcaaagcc gtagtgcatc atggacacca gaaatgcgag aaaaagcaag acaaaaagca
     2041 atgaaaggac gtaataaaaa tgaaaaagat aacagtaata ccacaaacaa ttaatccgct
     2101 cacgaataat ccgtttaata atgtagagag acgcaaagta gcggcttatg ctcgggtttc
     2161 aactgatagt gatgaacaaa tgacgtcact tgatgctcag attgactttt acactaatta
     2221 catcaaagca aatccagagt gggaattcgt taaagtatat tatgacgaag ggatttcagg
     2281 aactaacact aaaaaccgcg aagggttcaa acaaatgatc caagacgcca ttaatgggaa
     2341 aattgatctc attattacga aatcgattag tcggtttgct cgaaatacag tcgatacctt
     2401 agtggcgatt agaaaactta aagaaaagtg cgttgaatgc ttttttgaaa aagagaatat
     2461 ttatacattt gatagtaaag gggaactttt gattacgatc atgtcttcaa tcgctcagga
     2521 ggaatcaagg tccttatcac aaaacgtcac ttggggcatt cgcaaagcat tttctgatgg
     2581 caaggtccga atcgcctata agaacttttt aggttatcga aggggaacag atggtcgtcc
     2641 agaggtcatt cccgaagagg ccgaagccat cagaaaaatc taccggatgt ttcttaatgg
     2701 ttattcgatt aacgaaattg tgtatgcgct agaagaagat aacatcgact caccgagtgg
     2761 gagtaaaaca tggcgaattc aaacggtaca gagcatatta agaaatgaaa agtataaagg
     2821 tgacgcttta cttcaaaaga ctttcacggt cgattatttg gaaaagagaa cgaaagtcaa
     2881 tgaaggcgaa gttcctcagt attatgtaga aaattcacac gaagctatca ttgaaccgga
     2941 agaatgggaa atggtccaag aggaaattaa aagacgcgcc aaattaggca agcaatttcg
     3001 tggatcgaag gtttttgcga gccggctcgt ttgtgcggac tgtggggcgt tttacgggcc
     3061 gaaagtttgg cattccaccg acaagtaccg cagtgaagta tggcaatgta ataagcgttt
     3121 tgaaaaccgt aagctaggcg ttaaatgtga tacgccgtat ctagttgaag atcacattaa
     3181 gaaatctttt attaaagcat ttaacttgct tagtaaagac aaaaagaaag tgatcaacca
     3241 ctgtaaagag tttattgata tccttgataa cactgaggaa cttaatcgac ttattgacat
     3301 tcaaactacc gaagttgaag tgttggcaaa tatggcaaaa gtgctggttg aagagaacgc
     3361 cacgaccgca attgaccagg ccgattaccg aactcgatac gaagcgctcg aaaaaagata
     3421 tcaggaagag caggcaaatc ttgataacct tctttcagaa
//
LOCUS       DAOSEX010000065         3400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_25975_length_3400_cov_2.640060, whole genome shotgun sequence.
ACCESSION   DAOSEX010000065 DAOSEX010000000
VERSION     DAOSEX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25975_length_3400_cov_2.640060"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..414)
                     /gene="rpsT"
                     /locus_tag="PK612_01880"
     CDS             complement(148..414)
                     /gene="rpsT"
                     /locus_tag="PK612_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020208359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOH94895.1"
                     /translation="MPNIKQQIKRVKTNEKARQRNAHFKSMVRTQLKKVRVAIDAKDV
                     ALAEAEYKKAVSLLDKAAGRGVFHRNKVARHKSELARQINELSA"
     gene            complement(592..1059)
                     /locus_tag="PK612_01885"
     CDS             complement(592..1059)
                     /locus_tag="PK612_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94896.1"
                     /translation="MKYIKSSSHNIEFTSFNGYNLKHKLNSRKNKHIPKRIKKIIFAL
                     YSMVLWTTILVIYIFFKDPVILIYVLGAIMITLSLLILFLKDKELHVPCIHITMLIHT
                     ALFIYASVPLILGWNAYDMKASVVILIIFGISLLLGIFYYLHDFFARRKRLKL"
     gene            1102..2055
                     /gene="fmt"
                     /locus_tag="PK612_01890"
     CDS             1102..2055
                     /gene="fmt"
                     /locus_tag="PK612_01890"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOH94897.1"
                     /translation="MKDLRIIYLGTPDFSAELLRYLIEAGYNIIAVVTQGDSPVGRKR
                     VLTPSPVKTLALAHNIPVFTPLRIRKEYDFIVELEPDLILTFAYGQIVPKAVLEAPKY
                     GCLNFHGSILPKYRGASPIQMALINAEEETGVSLMEMVEAMDAGRVFGIEKFKIEPSD
                     NFLTIASKMVKASATLIEEVLPSVISGENKGVQQEESQVTFAPLLKIKDEWINLENDD
                     VKNVLGRIQAFAPATGASLSYDDLPLKIYEARFDNNNVTRTIGELFIRDKKLLLQVKG
                     GQIEVLKLQKSGKKIIDARSFINGEKHKLPTILKGYIDAEI"
     gene            2042..>3400
                     /locus_tag="PK612_01895"
     CDS             2042..>3400
                     /locus_tag="PK612_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOH94898.1"
                     /translation="MRKFNLSVHELVDFVLHSGDLDTRIFNLETMTRGTQIHAFYQAK
                     QNDKYLAEYALEYTFEYENHLITLQGRVDGVIVNGSEVTIEEIKSANTDIDKFFENNE
                     AWHLGQAKCYALMYALRFNLDVIDIQLTYISQIDERKMMKRYTFTREELQSDVFSYFG
                     EYLAFFYQLEKYEKRRTKRLKALNFPYKEVRSSQKVMMEQVEHALEHEHKIFLNAPTG
                     SGKTMAVLYPSLKVLAEKTNTKIFYLTAKGSGKAQAENALRVVREKALIKSTTVSAKE
                     KMCLNDKVACNPDECPYAVNYYGKIRNALLDAFKSRDEYNTEIVTEYAKKHELCPFEF
                     QLDLTLLSDVIIADYNYLFDPFVYLKRFFDSKRGNYIALVDEAHNLPRRVSDNYSLVL
                     DFTPFYSFRKVLKGAEHKKIRRIINRIVKDLDEYKEGIISEYETITEFPHELRSSLDR
                     FLV"
BASE COUNT         1162 a          519 c          636 g         1083 t
ORIGIN      
        1 ccgcgttaaa ctagcgatgg atcaaagacg acgcactagt ttagaagaag tatttaatat
       61 tttactcgcg cttgcggatt tagattataa aataaaaagc ggccaaattg accgttttta
      121 tggatttgaa atgtttcttc ttaattacta agcagaaagt tcattaattt gacgcgctaa
      181 ttcgctctta tgacgagcaa ctttattgcg atggaacaca ccacgacctg ctgctttgtc
      241 taataaagag acggcttttt tatattcagc ttcggctaag gcgacatctt ttgcatcaat
      301 cgctacgcga acttttttaa gttgagttcg caccattgat ttaaaatggg cgttgcgttg
      361 gcgcgctttt tcattggtct taacacgttt aatttgttgc ttaatatttg gcatactatt
      421 acctctttca ataataatct taaatattat ataaaattat aataatttta gcaaataaag
      481 agtgcaataa tttttcagta attgcgtgtt tctattcatt atattcatta aatttcttca
      541 aagtaggcaa tagagtaatt tatagcgata ctatgattaa aaaaacttta tttaaagttt
      601 aaggcgtttt ctccttgcaa aaaagtcatg caagtaataa aaaataccaa gtagtaaaga
      661 tatcccaaat attataagta tgacgacact agccttcata tcgtaagcat tccatccgag
      721 tattagcgga acagaagcat agataaaaag cgccgtatgg ataagcattg taatgtgaat
      781 acaaggaacg tgtaattcct tatcttttaa aaataaaata agtagtgaca atgtaatcat
      841 tattgcccct agcacatata ttaagataac tggatcttta aaaaatatat aaataacgag
      901 aattgtcgtc cataacacca ttgaatataa ggcaaagata atttttttaa ttcttttagg
      961 tatatgttta tttttcctag agttcaactt atgttttaaa ttatagccat taaagctagt
     1021 aaattcaata ttatgggacg aggatttaat atatttcatt acgaagtgtg aatttttgtc
     1081 tataatttaa gcgtgaagag tatgaaagat ttaagaatta tttatttagg aacaccagac
     1141 ttcagcgctg aacttttacg ctatttaatt gaagcaggtt ataacattat cgccgttgtc
     1201 acacaaggtg atagtcctgt tgggcgcaaa agagtattaa caccaagtcc cgtaaagact
     1261 ttagcattag cgcataatat tcccgttttt actccgcttc gcattcgtaa agaatatgat
     1321 ttcatagtgg aactagaacc ggacttaatc cttacttttg cctatggtca aattgtacct
     1381 aaagcagtac tagaagcacc taaatatggt tgtttaaatt ttcacggctc aattttgcca
     1441 aaatatcgcg gggcttctcc aattcaaatg gcactaataa atgccgaaga agaaacgggt
     1501 gttagtctta tggaaatggt tgaagcaatg gacgctggcc gagtgttcgg gattgaaaaa
     1561 tttaaaattg aaccaagcga taacttccta acaattgcaa gtaagatggt aaaagcaagt
     1621 gcgacactga ttgaagaagt tttaccaagt gttattagcg gcgaaaataa aggtgtgcag
     1681 caagaggaat cacaagttac ttttgccccg cttttaaaga ttaaagatga gtggattaat
     1741 cttgaaaatg acgatgttaa gaatgtttta gggcggatac aagcttttgc gcccgctaca
     1801 ggtgcgtcat taagttacga tgacttacca ttaaaaattt acgaagcacg ctttgacaat
     1861 aataatgtta ctcgcactat cggtgaactt tttattcgtg ataaaaaact tttattacaa
     1921 gtaaagggcg gacaaattga agtacttaaa ctgcaaaaaa gcggcaagaa aataattgat
     1981 gctcgtagtt ttattaatgg tgaaaagcat aaattaccga ccattttaaa aggatatatt
     2041 gatgcggaaa tttaacctca gtgttcacga gctcgtggac tttgttttac attctgggga
     2101 tttagatacc cgcattttta atttagaaac aatgacgcga ggtacacaga ttcacgcctt
     2161 ctatcaagct aaacaaaatg ataaatatct tgccgaatat gcacttgaat atacttttga
     2221 atatgaaaat catcttatta cattacaagg aagagtagac ggggttattg ttaatggaag
     2281 tgaggtaacg attgaagaaa ttaaaagtgc gaacactgat attgataaat tttttgaaaa
     2341 taatgaagcg tggcatcttg gacaagcaaa gtgttatgca ttgatgtatg cgctccgttt
     2401 taacttagac gttattgata ttcaattaac atatatttca caaattgatg aacgcaagat
     2461 gatgaaacgc tatacattta cgcgtgaaga attacaaagt gatgttttta gttattttgg
     2521 tgaatattta gcgtttttct atcaacttga aaaatatgaa aagcgccgta caaagagact
     2581 taaagcactt aattttcctt ataaggaagt acgttctagt caaaaagtaa tgatggaaca
     2641 agtagagcac gccttagaac atgagcataa aatattctta aatgcaccaa ctggtagcgg
     2701 taaaactatg gcggtacttt atcctagtct taaagtttta gcggaaaaaa ctaatactaa
     2761 aatcttttac ttaaccgcaa aaggaagtgg gaaggcacaa gccgaaaatg ctttacgagt
     2821 agtgcgcgaa aaagcactaa ttaaaagtac tacagtcagc gccaaagaaa agatgtgtct
     2881 taatgataaa gttgcctgta atcctgatga atgtccatat gccgttaatt attacgggaa
     2941 gattagaaac gcgctacttg atgcttttaa gagtagggac gaatataaca cagagattgt
     3001 tactgaatat gccaaaaaac atgaattatg tccgtttgaa tttcagttag atttaacact
     3061 tttgagtgat gttattattg ctgattacaa ttatctattc gatccttttg tgtatttaaa
     3121 gcgctttttc gatagtaaac gcggtaatta tattgccctt gttgatgaag cgcataactt
     3181 accacgccgt gtgagtgata attactcttt agtgcttgat tttacgccgt tctatagttt
     3241 tagaaaagtg ttaaaaggcg cggaacataa gaaaattaga cgaattatta atcggattgt
     3301 taaagacctt gatgaatata aagaggggat tattagtgag tatgaaacaa tcactgaatt
     3361 tccacatgaa ttacggagta gtcttgaccg ctttttagtt
//
LOCUS       DAOSEX010000066         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_26296_length_3371_cov_3.575995, whole genome shotgun sequence.
ACCESSION   DAOSEX010000066 DAOSEX010000000
VERSION     DAOSEX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26296_length_3371_cov_3.575995"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK612_01900"
     CDS             <1..193
                     /locus_tag="PK612_01900"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOH94899.1"
                     /translation="MPLAANIGDLFFSATKRHLAIKDYGSILVGHGGILDRVDSLLMV
                     SIVTAMLIILINNGWSILV"
     gene            206..1462
                     /locus_tag="PK612_01905"
     CDS             206..1462
                     /locus_tag="PK612_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOH94900.1"
                     /translation="MEFLSTILNIILFLVMLGVLITIHELGHFIAAKIFRVYVFEFAI
                     GFGPKIFRKKKGETYYSLRALPLGGYVAMLGEEDAVPDEFVNEEIPKERSLIGINRGK
                     RALIMSAGIIMNLVLGYAIFLFANGVLPQTGLSIRLEVKEDSPVYAMGIRSDDRIDFK
                     ETKIDGYTIQYFGEGKLDAAALEPEYYVLFQPKSLDDLTFGGESLVLLKIGATDLASE
                     NSFYTDLKDTDVLTFTATFLRGETYETAERIEKVLTLSTEPKNPDKLTDGYKFVSAGF
                     NLTKFKYRNNFSETFTAANKDFIRSVTAVARGLKSLFSDGLKNVSGPVGIFNISSQTL
                     KNHGLAPYLFLWGLISINLALFNLLPFPPLDGWHLLVVTVEAITRQEISPKFKQIASM
                     IGAILLIGLTLVILAKDIFGFVGVIL"
     gene            1463..3355
                     /locus_tag="PK612_01910"
     CDS             1463..3355
                     /locus_tag="PK612_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOH94901.1"
                     /translation="MGNVSARFFASINYENDKKIGLTFLEWKKVDEVWHYTLLKEKPW
                     NFEDYLDFSDALMSIPYAYKVSYAYTKLPRIEDALALFKPWHLHTYLSESNYVIMVVG
                     NDTLRFTFTDEVSALDAKAVLYEFSLLLKELSYNFALETVVISSAQEEDEEDEFSLGS
                     ERPFIEDDDDDEEYFDEEDDTGVADCDPLDPNENCERSLEYHDGLEDKLKENYEKMVK
                     ERQKRRHAKGAFNLTEIKDLEHKEVDEGVDFDGEIYSYDRRPTKHGVTYTLGIGKDAH
                     GISVRFYINKRGAKQDEVLKALKTGRFVRVQGYIAIDNFRGQTKIVRGHDIDEIAAPP
                     LPTDDYEGRKRIELHLHTKMSALDAVSTIDEYCELAKNMGHEAIALTDHGVVQAFPEA
                     QKAGKKYDIKIIYGSELYVIDETLKVVNNPAPIILNNARYVIFDLETTGLSARYNEII
                     EFGAVRVEHGLVTARLDILINPGIVVPEHITRITKITNAMLRGKPKLDDVLDEILEFL
                     GDDILVSHNAEFDLGFLNANLRKRGRNPLNNPTIDTLALSRYLFPESRGHRLGVLARN
                     LEVFYDEDGAHRADYDAEVLNSIWQAMLTKLTEDDQYLTHAALAELKLTQAALQYLWQ
                     RTLRCT"
BASE COUNT         1067 a          536 c          726 g         1042 t
ORIGIN      
        1 aatgccgctt gcggcaaata ttggtgactt attctttagc gctaccaaaa gacatttagc
       61 gattaaggat tatggttcga ttttagttgg tcatggcggt attttagacc gtgttgattc
      121 acttttaatg gttagtattg tgacggcaat gctaatcatc ttaataaata acggatggag
      181 tatactcgtc tagaaggaag ttagtatgga atttttaagt acaatattaa acattatttt
      241 atttttagta atgcttggtg tattaatcac cattcacgaa ttaggccact ttattgctgc
      301 gaagatattt agagtgtatg tctttgaatt tgcgattggg tttggtccta aaattttccg
      361 caagaaaaaa ggagaaactt attattcatt acgcgcattg ccgcttgggg gatatgttgc
      421 gatgctcggc gaagaagatg ctgtgcctga tgaatttgta aatgaagaaa ttcccaaaga
      481 acggagttta atcggcatta atcgtggcaa aagggcatta attatgtcag cgggtattat
      541 tatgaactta gtgttaggct acgctatatt cttattcgct aatggggttt taccgcaaac
      601 cgggttatca attcgtttag aagttaaaga agatagtcct gtatatgcaa tggggatacg
      661 cagtgatgac cgcattgatt ttaaagaaac gaagattgat ggttatacga ttcagtactt
      721 cggtgaaggg aagcttgacg ctgcagcact tgaaccagaa tattatgttt tatttcaacc
      781 aaagagttta gatgacttaa cttttggtgg tgagtcgctt gtccttttaa aaattggcgc
      841 gactgattta gcgagtgaaa attcgtttta cactgattta aaagatacag atgtgttaac
      901 ctttaccgca actttcttac gcggtgaaac ttatgaaact gcggagcgta ttgaaaaagt
      961 attaacactt agtactgaac caaaaaatcc cgataaatta acagacgggt ataaatttgt
     1021 gtccgctggt tttaatttaa cgaaatttaa atatcgcaat aactttagtg aaacattcac
     1081 cgccgcaaat aaagatttta tccgtagtgt tacggcagta gcgcgcggcc tcaagtcatt
     1141 atttagcgat ggtcttaaaa atgttagtgg accagtgggg atttttaata tttctagtca
     1201 aacacttaag aatcatggtt tagcacctta tttattctta tggggtttaa ttagtattaa
     1261 cttagcgctc tttaacttat taccgtttcc accacttgat ggatggcact tattagtggt
     1321 aacagttgaa gcgattactc gtcaagaaat tagtcctaaa tttaaacaaa ttgcctcaat
     1381 gattggtgca attcttttga ttggcttaac acttgttatt ttagcgaaag atatttttgg
     1441 cttcgtagga gttattttat agatgggaaa cgttagtgct cgtttttttg cgtcaattaa
     1501 ttatgaaaac gacaagaaaa ttgggttaac gtttttagaa tggaaaaaag ttgatgaagt
     1561 gtggcactac acacttttaa aagaaaaacc gtggaatttt gaagactatc tagattttag
     1621 tgatgcgtta atgagtattc cttatgcata taaagtgagt tatgcttaca ctaaattacc
     1681 gcgtattgaa gatgctttag cgctttttaa accgtggcat ttacatactt atcttagtga
     1741 aagcaactat gttattatgg tcgttggcaa tgatacatta cgctttactt ttaccgatga
     1801 agttagtgcg cttgacgcta aagcggtgct ttatgaattt tctttactct taaaagaact
     1861 atcttataac ttcgccttag aaacagttgt cattagtagt gcacaggaag aagacgagga
     1921 agacgaattt tcacttggga gtgaaagacc ctttatcgaa gatgatgatg acgatgaaga
     1981 atattttgat gaagaagatg acacgggtgt agcagattgc gatcctctag atccaaatga
     2041 aaactgtgaa agaagtcttg aatatcacga cgggttagaa gataaactaa aagaaaatta
     2101 cgaaaaaatg gttaaagagc ggcaaaaaag aaggcacgct aaaggagctt ttaatcttac
     2161 cgaaattaaa gatttagaac ataaagaagt tgatgaaggt gtggactttg acggtgaaat
     2221 atatagttat gataggcgtc cgacaaagca cggtgtcact tatacactcg gtattggcaa
     2281 agacgctcac ggtatttcag tacgatttta tattaataaa cgtggcgcaa agcaagatga
     2341 agtgttaaaa gcactaaaga cgggacgctt tgtgcgtgta caaggttata tcgctattga
     2401 taatttccgc ggtcaaacta aaattgttag agggcacgac attgatgaaa ttgctgcacc
     2461 accattaccg acagatgatt acgaaggtcg gaaacgcatc gaattacatc tacacactaa
     2521 aatgtcagcg cttgatgccg taagtacgat tgatgaatat tgtgaacttg ctaaaaatat
     2581 gggccatgaa gcgattgcac ttactgatca cggtgttgtg caagcctttc ctgaagccca
     2641 aaaagccggg aagaaatatg atattaaaat aatttatggt agtgaactat atgttatcga
     2701 tgaaacatta aaagtcgtta ataaccctgc accaattatt ttaaataatg cccgatatgt
     2761 aatttttgac ttagaaacaa cagggctaag tgctcgctat aacgaaatta ttgaatttgg
     2821 cgctgtgcgt gtggagcacg gcctagtgac agcgcggctt gatattttaa ttaatccagg
     2881 tattgtcgtt cctgaacata ttacacgcat tacaaaaatt acaaatgcga tgctgcgcgg
     2941 taaacctaaa ctagatgatg tccttgatga gattttagag tttttaggtg atgacatttt
     3001 agtgtcacat aatgccgagt ttgacttagg atttttaaac gctaatttgc gtaaaagagg
     3061 acggaacccg cttaacaatc caacaattga cactttagcg ttatcgcgct atttattccc
     3121 agaatcacgt ggtcaccgat taggtgtttt agcccgcaat ttagaggtat tttatgatga
     3181 agacggggcg caccgtgcgg attacgatgc ggaagtttta aatagtattt ggcaagcaat
     3241 gcttacaaaa ttaacggaag acgatcaata tttaacacat gccgcccttg cggagttaaa
     3301 actaacacaa gctgcgctcc aatacttatg gcagcgcacg ctacggtgta cgtgaaaaat
     3361 aaagacggac t
//
LOCUS       DAOSEX010000067         3349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_26552_length_3349_cov_2.580146, whole genome shotgun sequence.
ACCESSION   DAOSEX010000067 DAOSEX010000000
VERSION     DAOSEX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26552_length_3349_cov_2.580146"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PK612_01915"
     CDS             complement(<1..1296)
                     /locus_tag="PK612_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH94902.1"
                     /translation="MSLFLLTNGLIIFEATLANGASGARSSLLSLILSVFVNKTVDGP
                     ETAYVPIESLTLKTKDDTTLTEGMHFYIPLGITRRITATVLPENATDKGVNWTSSDSS
                     ALEVYPGGYLEARALSENVLVTAMTNSPLHRISFYVTIHEKVAPPEYEVALTKDRITI
                     DTTTQLEVSVDESVANEYDVRKLTFFSEDESVATINKYGVIKGISVGETYVGVVGDDR
                     KFKVSVKSNDTPLISPTSITLEFDEIAYVYDKIPLNYTFDVENVTDPSLTFVSSNDIV
                     ATVIKEDDNYFIKTPKIKGTATITAYLNSNFAIRATKDIIVNNVMPTSVNFTNVPTEL
                     ITGQRVNVGYDLNHSLAKESLSVTDKRVIFTSSDNSIARVSTGNMFGTIVALKEGVVT
                     ISATSVADPTISASFSIKIIPRPFINDDNFSDFHGFVRKA"
     gene            1448..2635
                     /locus_tag="PK612_01920"
     CDS             1448..2635
                     /locus_tag="PK612_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94903.1"
                     /translation="MKKCQLILLPLLTMLLSGCTPLSLSEVLKPSDNSLSETDVPSTE
                     SSSEIEGAQTWSVDDDFTWQTYEYALDSYNVPLYVPTENPYIDIISDADKETFYTNNY
                     KRASSYEDAMFRTEKGLISGDIIDTPNTANYPLNHLPNRVYRTLANYRINEGVYEYTL
                     DGEYKSYTINNINGKVKKVYYGAAYVSLDDVAAYLFAFGEAPANQMMAKNSTAMNQAI
                     SLFGKYGRVNNGYYSSDVTKYKYEPSLPHTDNGGPVEADMYNYREMDFGYTYTPWGYG
                     IESDDPYNNGTKITRSTVRFVYTAYDNEGNEGAKYIPVEHRHVFLTINHYNDFFEYLN
                     YEGGWSESFGWMSAGNEYCASMNSSKYGKGYYDFENPVPPTKYNIPQVKTLTELQSIF
                     NCL"
     gene            complement(2910..3059)
                     /locus_tag="PK612_01925"
     CDS             complement(2910..3059)
                     /locus_tag="PK612_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94904.1"
                     /translation="MNIKRFLMLLINAFVLFVILFMLTRTLWTLLVFVMPVVSLFLPK
                     EKSNG"
     gene            complement(3049..>3349)
                     /locus_tag="PK612_01930"
     CDS             complement(3049..>3349)
                     /locus_tag="PK612_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOH94905.1"
                     /translation="LIENKDKVKGIIGERISHQEVDTGFFNAMVESTVSEAFFAGHDH
                     LNNFTFEKDGIILGYGQISGYNGYGTLRRGARIIEIDESKMFKTYVVYEDLSYEY"
BASE COUNT         1160 a          581 c          575 g         1033 t
ORIGIN      
        1 tgcttttcga acaaagccgt ggaagtcact aaaattatca tcattaataa agggtcgcgg
       61 aataatttta atactaaaag atgcgctgat agtagggtcg gcaacacttg tcgcgctaat
      121 agtaacaacg ccttctttta aagcaacaat agtgccaaac atgttcccag ttgatacgcg
      181 cgcaatactg ttatcacttg aagtaaaaat aacacgctta tcagtcacac ttaacgattc
      241 tttcgctaag gaatgattta agtcataacc gacatttacc cgctgaccag taattaattc
      301 agttggaaca ttagtaaaat taacagaagt tggcatcaca ttattaacga taatatcttt
      361 tgtggctctg attgcaaagt tactatttaa gtaagcagta attgtagcgg taccttttat
      421 tttaggtgtc ttaataaagt aattatcatc ttctttaata acagtagcaa caatgtcatt
      481 acttgataca aaagttagtg atggatcagt aacattttcc acgtcaaatg tataatttag
      541 ggggattttg tcgtaaacat aagcgatttc atcaaattct aaagtaattg aagttggcga
      601 tattagcggt gtatcattac tcttcacact caccttaaat tttcggtcgt caccaacaac
      661 accaacataa gtttcgccaa cgctaatccc tttaataaca ccgtacttat taatagttgc
      721 aacactttcg tcttcactaa agaaagtgag tttacgaaca tcatattcat tagcgacact
      781 ttcatcaaca ctaacttcaa gttgtgtagt agtgtcaata gtaatcctat ctttagtaag
      841 agcgacttcg tattcagggg gcgcaacttt ttcgtgaata gtgacataaa aactaatgcg
      901 atgcagcgga ctattagtca tagcggtgac taatacattt tcacttagtg cgcgcgcttc
      961 taagtaacca cccggataaa cttcaagcgc actactatca ctacttgtcc aatttacccc
     1021 tttatccgta gcattttctg gtaatacagt tgcagtgatg cgtctagtga tgcctagcgg
     1081 tatatagaag tgcatacctt cagttaaagt agtgtcatct ttagttttta gtgttaatga
     1141 ttcaatagga acataagctg tttcaggacc gtccactgtt ttattaacaa acacacttaa
     1201 aataagcgag agtaaacttg agcgcgctcc agaagcacca ttcgcgagtg ttgcttcaaa
     1261 aataataagc ccgtttgtta ataaaaatag gctcaagata aagtaacgaa tatttctttt
     1321 aagccagttc atgtttatat tatagcaatt agattgatta aaaatagtcg catactacaa
     1381 ctaaagttgt aatctaacat gttttagtta ataataaatg acagacttgt cataaagagg
     1441 ttagatcatg aaaaaatgtc aattaatatt attaccatta ttaactatgc ttttaagcgg
     1501 ctgtacgcct ttaagtttaa gtgaagtatt aaagccttct gataactccc taagtgaaac
     1561 agatgtaccg tcaactgaaa gtagtagcga aatagaagga gcacaaactt ggtcagtgga
     1621 tgatgacttt acttggcaaa catatgaata cgctcttgat agttataatg tgcctctata
     1681 tgttccaaca gaaaatccgt atatagatat tataagtgac gctgataaag aaacatttta
     1741 cacaaacaat tataaaagag catcatctta cgaagacgca atgtttcgga cagaaaaagg
     1801 tttaatctct ggcgatataa ttgacactcc aaacacggca aactatccgc ttaaccacct
     1861 tcctaatcga gtatatcgca ctctagcgaa ttaccggatt aatgaaggag tgtatgaata
     1921 tacattagat ggtgaatata agtcatacac aattaacaat attaatggca aagtaaagaa
     1981 agtctattac ggggctgctt atgtttcgct tgatgatgtt gcggcttatt tatttgcttt
     2041 tggtgaagca cctgcgaatc aaatgatggc caaaaatagt actgcaatga atcaagcaat
     2101 atccttattt ggtaaatatg gacgcgttaa taacggttat tacagcagtg atgttactaa
     2161 atataaatat gaaccttctt taccgcacac tgataatggt ggaccggttg aagcggatat
     2221 gtataattat cgcgaaatgg attttggtta cacttatacg ccgtggggat atgggattga
     2281 aagtgacgat ccatataata acggcactaa aattacacgt agcactgtac gctttgttta
     2341 tactgcatat gataacgagg ggaacgaagg cgctaaatat attccagttg aacatcggca
     2401 tgtgttctta acaattaatc actataatga cttctttgaa tatttaaatt atgaaggcgg
     2461 atggagcgag tcatttggtt ggatgagcgc cggtaatgaa tattgcgcta gtatgaactc
     2521 gagtaaatac ggtaaaggat attacgattt tgaaaatcct gtgccaccaa caaaatacaa
     2581 tattcctcaa gtaaaaacac ttactgaatt acagtcgatt ttcaattgct tatagtcttt
     2641 acaatatgta catatgaata gcaaagccaa aggcaaagat gctcaaggct aaatatataa
     2701 aaagcagtgt attataaagt tttcgatatt tattttcttt tgaaataaag taataataac
     2761 ttaaagaaaa gatgtcaatt atgattaata ttttgtgttt tagatcgttt ttgcttggaa
     2821 ataccgacaa aatacgcacg aaaagtgtaa taggaataaa agtaaagaca atgatgtaaa
     2881 aagatagttg taagaaccca aagaataact caaccattac ttttctcctt tggtaaaaat
     2941 agtgaaacaa ctggcatcac aaatactaag agcgtccata atgttcttgt taacataaat
     3001 aaaataacaa agagaacaaa agcattaatt aaaagcatta agaatcgttt aatattcata
     3061 gcttaaatcc tcatatacaa catatgtctt aaacatttta gactcatcaa tttcgatgat
     3121 gcgcgctccg cgtctaagtg tgccgtagcc attatagcca cttatttgcc cgtatcctaa
     3181 aataattcca tctttttcaa aagtgaaatt attaagatga tcatgtccag caaagaaggc
     3241 ttcactaaca gtactttcaa ccattgcatt aaagaaacct gtatcaactt cctgatgact
     3301 aatacgctcg ccaataatgc ctttaacctt atctttattt tctattaat
//
LOCUS       DAOSEX010000068         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_27039_length_3305_cov_3.842462, whole genome shotgun sequence.
ACCESSION   DAOSEX010000068 DAOSEX010000000
VERSION     DAOSEX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27039_length_3305_cov_3.842462"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..724)
                     /locus_tag="PK612_01935"
     CDS             complement(188..724)
                     /locus_tag="PK612_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOH94906.1"
                     /translation="MEKEVMVNGKTYTLIKLLGKGKGGYSYLATFNNKEVVVKMIHHE
                     PCEYYTFGNKIEAEYYDYQRLFKAGIRMPRLIDICKGQEIIIKEYVKGDTIFEYVKRD
                     VNIDLFIDQVREMAALAKKAGLNIDYYPTNFVVQDNLIYYVDYECNEFSEEWNFENWG
                     IKYWTRTKEFKAVLKKEQ"
     gene            809..2287
                     /locus_tag="PK612_01940"
     CDS             809..2287
                     /locus_tag="PK612_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOH94907.1"
                     /translation="MKIISKSNYTLFKNCPKSLWLSFYKSDVAAPLTMDVLKRIEDGK
                     EVGDLAKKYFANTIDVKSLLPDGSLDISKMLELTKKYLKQKGVTIAEASFKVNNLFCS
                     IDLLRVTSEGYEIYEVKSTTEAKVEHKVDAAFQKYVLQQYGLNVINTYILHLNKDYIR
                     KGDLELDQLFTMNHLDEDTSFIEAMHNMKSDLKAIDKLLLTKAEPAETLLTRCKNCDF
                     KDYCQKDIPVPSVLDVYRLSGYKYLNKGIVTFEDLLESNVALRKRQSIQVHAYLDNKE
                     VIIDKKGLASFLKKIKYPVYHLDFESYQMPIPPCNLMKPYDQIPTQYSLHIEYEDGSL
                     EHKAFLGNSLDPRREIAESLCANIPRNACVTAYYKGFECGRLNELATLFPDLEEHLLN
                     ISKNVIDLLEPFESGYYYHKNMGKSNSIKAVLPALYPDDSELDYSALPVVHNGSEAMV
                     MYPIMLGAKKEEKERIRNGLLEYCRLDTLAMVKLLQKLRKTQ"
     gene            complement(2291..3202)
                     /locus_tag="PK612_01945"
     CDS             complement(2291..3202)
                     /locus_tag="PK612_01945"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOH94908.1"
                     /translation="MKKVFVLGSINYDVVVYTDKMPILGESIIGQGMIANLGGKGANH
                     AIAAKKIGVDKVFLISTVGDDDSGHIMLKSIKEHGVDTSGIEVIKGEPSGTCFIIFNR
                     SAEDNAVIVGNGANLMNAPQKAIDLLKKYARKGDIFIAQLETNLEALYKGIDFAHELG
                     MYIIINPSPVCEFNKSILSKVDLLILNAAEAKLLTGVNYKNNNDLQEIHDKLKAKSTI
                     VTLGEDGAYLIRDNEIIYQLAIPTNVVDTTCAGDTFLGALAYRKANGYEIEDSLRFAA
                     TASSIAVSRKGATQSVPYLNEILELYK"
BASE COUNT         1057 a          637 c          499 g         1112 t
ORIGIN      
        1 tgctattttc acttccctta aacatcaagc gatagtcttt aattattgct ttaccaactt
       61 ttttcgatgt tccacaccgc tcctgcattt gtgcaagatt aagattgctg ccgtaggcta
      121 aatagtaccg caagtctttg tgactaaaat cattaaaatt cataatattt tccccattac
      181 cttgtattta ttgttctttt tttagcactg ctttaaattc cttcgtgcgt gtccaatatt
      241 taatgcccca attttcaaaa ttccattctt cgctgaactc attacattcg taatcaacgt
      301 aatatattaa gttgtcttgc acaacaaaat tcgtggggta gtaatcaata tttaggcccg
      361 cttttttggc taaagctgcc atttctcgaa cttgatcaat aaataaatcg atatttacat
      421 ctctttttac atattcaaaa attgtgtcgc ctttaacata ttctttaatg ataatctctt
      481 ggcctttaca aatatcaatt aagcgcggca ttctaatgcc cgccttgaat aatcgttgat
      541 aatcgtaata ttctgcctca atcttgttgc caaaagtata atattcacat ggttcatgat
      601 gaatcatttt aaccacgact tctttattat taaaagtcgc taaataggaa taaccacctt
      661 tccctttacc gagcaatttt attagtgtgt aagttttacc gtttaccatt acttcttttt
      721 ccatagttca ccatcagtat agcgttttta acccttaaac taaagaccac tatcgctttt
      781 gaaggtgaaa gctggtaaaa taaataatat gaagattata tcgaaaagta attacacact
      841 atttaaaaat tgtccgaagt cactgtggtt atctttttat aaaagtgatg tcgcagcgcc
      901 tttaaccatg gacgttttaa aacgaattga agacggaaaa gaggtcggag accttgctaa
      961 aaagtatttt gcaaacacaa ttgatgttaa gtctttattg ccagatggtt cgctagacat
     1021 ttctaaaatg cttgaactaa ctaaaaaata tttaaaacaa aaaggtgtca caatcgctga
     1081 agcttctttt aaagtaaata acctcttctg ttctatagac ttattacgcg tcactagtga
     1141 aggctacgaa atttatgaag ttaaatcaac gaccgaagca aaagtagaac ataaggttga
     1201 cgctgcattt caaaagtatg tattacaaca atatggacta aatgttatta acacatatat
     1261 tctgcattta aataaagact atattcgtaa aggtgattta gaattagatc aactatttac
     1321 catgaatcat ctagatgaag acacatcttt catagaagca atgcacaata tgaaaagtga
     1381 tttaaaagct attgataaat tactgttaac aaaagctgaa cccgctgaaa cattattaac
     1441 aagatgtaaa aattgtgact ttaaagatta ttgtcaaaag gatattcctg ttccaagcgt
     1501 actagatgtt taccgcctat caggttacaa atatttaaat aaaggcatcg tcacttttga
     1561 agatttatta gaaagcaatg ttgctttgcg aaaacgacaa agtattcaag ttcatgccta
     1621 tttagataat aaagaagtca ttatcgataa aaaagggctt gcctcttttc taaagaaaat
     1681 taagtatcct gtctatcatt tagattttga atcctaccaa atgccaatcc caccatgtaa
     1741 ccttatgaag ccatacgacc aaattcccac acaatattca ttacatattg aatatgagga
     1801 cggaagttta gaacataaag cctttcttgg taattcactt gatccgcgtc gcgaaattgc
     1861 tgagtcatta tgcgctaata ttccgcgtaa tgcatgcgtt actgcctatt ataaaggctt
     1921 tgagtgtggt cgcttaaatg aactcgccac attatttcct gatttagaag agcatttact
     1981 taacataagt aagaatgtca tagatctatt agaaccattc gaaagcggat attattacca
     2041 taaaaacatg ggtaaaagta actccatcaa ggcggtttta ccagcgctat atcctgatga
     2101 ttctgaactt gattatagtg cgctgcccgt tgtccataac gggagcgagg ctatggttat
     2161 gtatccaata atgctaggag caaagaagga agaaaaagaa agaatccgta acggactctt
     2221 agaatattgt cgcttagata ctttagcaat ggttaaactc ttgcaaaaat tacgaaaaac
     2281 acaataagtg ttatttatat agttcaagta tttcattaag gtagggcact gattgggttg
     2341 ccccttttct tgatactgca atacttgaag cggtcgcggc aaagcgtaat gaatcttcta
     2401 tttcgtaacc atttgctttc cgatacgcta atgcacctaa aaaagtatca cccgcacatg
     2461 tcgtgtccac aacatttgtg ggtattgcca gctgataaat tatttcgtta tctctaatta
     2521 aatacgcacc atcttcgcca agtgtaacaa tcgttgactt tgcttttaat ttatcgtgta
     2581 tttcctgcaa atcgttgtta tttttataat taacccctgt taataacttg gcttctgccg
     2641 catttaaaat taataaatct actttcgaca atatgctttt attaaattca cagactggtg
     2701 aaggattaat aataatatac attcctaatt catgtgcaaa atctattcct ttatatagcg
     2761 cttctaaatt tgtttctaat tgtgcgatga atatatcgcc tttacgcgca tatttcttta
     2821 ataagtcaat tgctttttgg ggggcattca tcaaattagc gccattcccg acgatgacag
     2881 cattatcttc cgcgctacga ttaaaaataa taaaacatgt cccgctaggc tcgcctttaa
     2941 ttacttcaat cccgctagtg tctacaccat gttctttaat gcttttaagc atgatatgac
     3001 cactatcgtc atcaccaacc gtactaatta aaaatacttt atcaacacca atctttttcg
     3061 ccgcaatcgc gtgattagcg cccttgcccc ctaaattagc aatcattcct tgaccaatta
     3121 ttgattcacc aagaatcggc attttatcgg tatacactac tacatcatag ttaattgatc
     3181 ctaacacgaa tactttcttc attacttaac tgcctccgtt attttttaca cactacattc
     3241 ggaaatattt agtgttagat gatttgccaa ttttcctgac tttaccactc ttaaccattt
     3301 ttcct
//
LOCUS       DAOSEX010000069         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_27123_length_3299_cov_3.298089, whole genome shotgun sequence.
ACCESSION   DAOSEX010000069 DAOSEX010000000
VERSION     DAOSEX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27123_length_3299_cov_3.298089"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..147
                     /locus_tag="PK612_01950"
     CDS             58..147
                     /locus_tag="PK612_01950"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94909.1"
                     /translation="MKQWGLVLKMFANENGGSFPTTNQWMVNG"
     gene            complement(125..925)
                     /locus_tag="PK612_01955"
                     /pseudo
     CDS             complement(125..925)
                     /locus_tag="PK612_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
     gene            1093..2634
                     /locus_tag="PK612_01960"
     CDS             1093..2634
                     /locus_tag="PK612_01960"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOH94910.1"
                     /translation="MEKKEKLVLAVDFGTQSLRVSIVNQFGEIKAIIKNKYNEAYFSS
                     EPSFAEQNPNYYWYELCNATYKLKEKHDDLLQKVLAMSVTTFRDSPVFLDKNYEPTRP
                     MVLWLDQRQAALHKKPPFLRRLIFAIVGMSETVTLNMKRTPAIWVQENEKDVWEKTAY
                     YVNFSTYINYKLIGELIDSVANQTGHFPIHFKKGKWYGDNALKNIYRVPRTKLAKIVK
                     QGTVMGVISEAASKATGLPQGLKIIAAGTDKGSEAFGTGAYRKDIASISYGTASSISV
                     PNKKYIEPELFLPAYSAPIDGLYQMEVQIYRGYWMIGWFIKQFGTHETLEAKIETMAT
                     EEVLNYKMLEIPPGSQGLVLQPYWGPGLKRPEARGAIVGFSDYHTRIHVYRAIIEGIA
                     FGLKEGLLGIEKRQHKKVKEIRVSGGGSQSDAICQITADIFGVPVSRTQTYETATIGT
                     ALVTFYYLGVFDSLETATKAMVHIEKTFIPNPKNVKQYNKLFKDVYAKLYPKLKGIYK
                     KLKYF"
     gene            complement(2692..>3299)
                     /locus_tag="PK612_01965"
     CDS             complement(2692..>3299)
                     /locus_tag="PK612_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH94911.1"
                     /translation="MRFYEINGGSITLDGTDIRNYSRSALRRSVGMVLQDTWLFKGSI
                     KNNIRYGNREATDEEVIEAAKAARAHHFITTFPGEYEFILNEDGTNISQGQRQLITIA
                     RAIISKPKILILDEATSSVDTRTEYAIQSVMNEIMKDRTTFIIAHRLSTIKNAKKILV
                     MNKGNIIETGTHEELLAANGFYAGLYNAQFLGIDDSQETVD"
BASE COUNT         1092 a          620 c          616 g          971 t
ORIGIN      
        1 acgccggcgc tcgcacgtgc gcgcgaggcg gcgcggcggg cgtcctgtgc gaacaatctg
       61 aagcagtggg gactggtgct gaagatgttc gcgaatgaga acggggggag tttcccgacg
      121 accaaccagt ggatggtgaa cgggtaaagg caacaccagt tccacttgta tcacccttgt
      181 taccataaac cattgattgc acgtttacag cagtacccca ttcgtaagga atgtggttca
      241 ttctacgata gatatttgct ctttcgttat cccagctacg gaagactgcg gtaacagctt
      301 caattaattg gacacgagcg tcacttggga attcttcacc aagaactttt ttataatgag
      361 ctttaaattg tttaactaat tctcttaaat gttcagcctt taattcacta tcaagcttaa
      421 caccagcttt aactttcatg tcttcaagca ttttttccat ttcggcacgt ggttggcctt
      481 taacaacgtt gaaatacatt aaaataaaac gacgatagct gtcaaacgca aatgctgcgt
      541 taccagtttc atttgctaaa acttcggcaa cttcatcatt gagtcctaag tttaaaacag
      601 tgtccatcat cccaggcatt gatacacgag caccagaacg aacacttacg agtagtggat
      661 ttttcttacc accaaatgtt ttaccagttt ctttttctaa ttctgtaact gcattgtaaa
      721 tttgatcaat cacaaattgt ggtaattttt taccttcatt gtaataaagg ttgcaagctt
      781 cagttgtgac ggtgaaccct tgtggaatag gaacgccaat gcgtgtcatt tcagcaaggt
      841 tagcaccttt accgcccaag taattccggc ctaatttttc gccttcttta cacttgaacg
      901 caaagacata tcttttttct gccatatttt tcctcctttt aataagcagt atttaacata
      961 ccatcgctaa ttataatatc atagatatta aggtttatta caataaatca ctcaaaaaca
     1021 gcgaaaattg aagagaaaac actataatag taataatttg ctaaaatagt aatgctaaag
     1081 gaaaaatcga atatggaaaa gaaagaaaaa cttgtactag cagtcgactt tggtacccaa
     1141 tctctaagag tctcgattgt aaaccaattt ggagaaatca aagcgatcat taaaaataaa
     1201 tataacgagg cttattttag tagtgagcct agttttgctg agcaaaatcc aaactactat
     1261 tggtatgaat tatgtaacgc cacatataaa ttaaaagaaa agcatgatga ccttttacag
     1321 aaagtactag cgatgagtgt cacgactttc cgtgatagcc cagtatttct tgataaaaac
     1381 tatgaaccaa ctagaccaat ggtgttgtgg cttgatcaac gtcaagcagc gctccataag
     1441 aaaccaccat ttttaagacg cttaattttc gctattgttg ggatgagtga aacggtcact
     1501 ttaaatatga aacgcacacc agcgatatgg gtgcaagaaa atgaaaaaga tgtttgggaa
     1561 aaaacagcgt attatgttaa tttctctact tacattaact ataaattaat tggtgaactt
     1621 atcgatagcg tcgctaatca aacaggtcac ttcccaatcc attttaaaaa agggaaatgg
     1681 tatggtgata atgcattaaa gaatatttat cgtgttccgc gcactaaatt agcgaaaatt
     1741 gtaaaacaag gaacggtaat gggtgttata agcgaagccg caagcaaagc aacaggactt
     1801 ccgcaaggat taaaaatcat tgcagcggga actgataaag gaagcgaagc atttggaaca
     1861 ggtgcgtacc gcaaagatat tgcttcaatt tcctatggca ctgcaagttc aatatctgtt
     1921 ccgaataaaa aatacattga acccgaatta tttttaccag cctatagcgc accaattgat
     1981 ggtctttatc aaatggaagt gcaaatatat cgtggctact ggatgattgg ctggtttatt
     2041 aaacaatttg gtacgcatga aacacttgaa gccaaaattg aaacaatggc cactgaagaa
     2101 gtgttaaatt ataaaatgct tgaaatacca ccaggctcac aaggactcgt cttacaacca
     2161 tattggggtc caggacttaa acgcccggaa gctcgtggtg cgattgttgg atttagtgat
     2221 tatcacacac gcattcatgt ttatcgggcg attattgaag ggattgcttt tggtttaaaa
     2281 gaagggttac tcggtattga aaaaagacaa cataaaaaag taaaagaaat aagagtatca
     2341 ggaggcggtt cgcaaagtga tgcgatatgt caaattaccg ctgatatatt tggtgtccca
     2401 gttagtcgga cacaaactta tgaaaccgca acaattggta cagcgcttgt tactttctat
     2461 tatttaggtg tgtttgattc acttgaaaca gccacaaaag cgatggtcca cattgaaaaa
     2521 acatttatac caaatcctaa aaatgttaaa cagtataata aattatttaa agatgtatac
     2581 gctaaacttt atccaaaatt aaaaggcata tataagaaat taaaatactt ctagaagaaa
     2641 aaataaaagg gttaatcgac aaattaaccc ttttttgctg taaataattg attaatccac
     2701 agtttcttgg ctgtcatcaa taccaaggaa ttgtgcatta taaagaccag cgtaaaatcc
     2761 gtttgctgct aaaagttcct cgtgagttcc cgtttcaata atgttacctt tattcattac
     2821 caaaatcttc ttagcatttt taatggtgct taagcggtgg gcaataataa atgtagtgcg
     2881 gtctttcata atttcattca taacactttg aatcgcgtat tccgtacgcg tatcaacact
     2941 actagtagct tcatcaagaa taaggatttt aggtttagaa ataattgcac gagcaatcgt
     3001 aattaactgg cgttgccctt gcgaaatatt agtgccatct tcattaagta taaactcgta
     3061 ttcacccgga aaagtagtta taaagtggtg agcgcgcgct gctttagcag cttcgataac
     3121 ttcttcgtca gtagcttcac gattaccata acgaatatta tttttaatcg aacctttgaa
     3181 gagccaagta tcttgtaaaa ccatcccaac actacggcgt aatgcacttc gtgaataatt
     3241 acgaatatca gtaccatcta atgtaattga accaccattt atttcataga atcgcataa
//
LOCUS       DAOSEX010000070         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_27204_length_3292_cov_3.538462, whole genome shotgun sequence.
ACCESSION   DAOSEX010000070 DAOSEX010000000
VERSION     DAOSEX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27204_length_3292_cov_3.538462"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1252
                     /locus_tag="PK612_01970"
     CDS             <1..1252
                     /locus_tag="PK612_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH94912.1"
                     /translation="FFSMSGSDFVEMFVGVGAGRVRDMFQQAKKTAPCLIFIDELDAV
                     GRQRGAGLGGGNDEREQTLNQLLIEMDGFSDNSGIIVLAATNRDDVLDPALLRAGRFD
                     RKITVSLPDRAGRTAIFGVHARGKIIDESIDFDAISRRTVGFSGADIESVLNDAAILA
                     IRDNADKIMLRHIDEAIDRRIAGPARTSRQLNEHERKVIAYHEAGHAVVGLNHPFSDK
                     VQKITIIPRGQTGGHVLMTPEEDRFLMTKNQLIARIVGYLGGRTSEEVFFDDVSTGAQ
                     NDIEVATRIARLMVTEFGMSDLGPIQYERDTGSVFLGRDYTSSQKNFSQQVALEIDKE
                     VRKIIEQAHDKAREIIEKHKDDVILIAETLLDRETITAEQIDRLLKDRKLDEPKQAVI
                     GEVVLTEKPKVEPTPTPVPEDDKK"
     gene            1291..2271
                     /gene="dusB"
                     /locus_tag="PK612_01975"
     CDS             1291..2271
                     /gene="dusB"
                     /locus_tag="PK612_01975"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOH94913.1"
                     /translation="MWKIGNIEIKGKTILAPMAGITTFAYREFMRSFGVALCYTEMIS
                     DSGLIFNNEKTYEYLPRPEEKRPTSVQLFGGETTKLLVAIEKIEKHTDNYDFLDLNLG
                     CPVPKVTRNNGGSAWLKHPNELIAMVEKVVKRSTKPVTVKIRIGWDEKHINFKELLPK
                     LEEAGVSLIAIHLRTTKQLYGGKARYDLAQNIQNLVSIPLVVSGDIYTLDDAIKALEI
                     TGAQAVMLARGQLGNPFLATQIETYFKTGERLKDPSLYQQLDYLWTLANMMVEEKGEH
                     MGILVMRGIAHHFIKGFPFTKTYKVKLTKMSTLNDLELIIKEIKNQALLI"
     gene            2312..3292
                     /locus_tag="PK612_01980"
                     /pseudo
     CDS             2312..3292
                     /locus_tag="PK612_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057432.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
BASE COUNT         1111 a          549 c          717 g          915 t
ORIGIN      
        1 tttcttcagc atgtcgggtt ctgactttgt tgaaatgttt gttggtgttg gtgcaggtcg
       61 cgttagagac atgttccaac aagcaaagaa aacagcacct tgcttaattt tcattgatga
      121 attagacgct gttggtcgtc aacgtggtgc cggacttggt ggtggaaatg acgaacgcga
      181 acaaacgctt aaccaacttt taattgagat ggatggtttt agtgataact caggaattat
      241 tgttcttgcg gcgacaaacc gcgatgacgt tttagaccca gccttattac gtgcaggacg
      301 gtttgaccgt aaaattaccg ttagtttacc ggatcgcgct ggtagaacag caatctttgg
      361 tgttcatgct cgcggtaaaa ttattgatga atcaattgac tttgatgcca tttcacgccg
      421 gactgttgga ttttcaggcg cagatattga aagcgtgtta aatgatgcag cgatcttagc
      481 aattcgcgat aatgctgaca aaattatgtt acggcatatt gatgaagcta ttgatcgtcg
      541 tattgctggt cctgcccgca caagccgaca acttaatgaa cacgaacgca aagttattgc
      601 ttatcacgaa gcgggtcacg ctgttgtggg gctaaatcac ccatttagtg ataaagtgca
      661 aaaaattaca attattccac gtggtcaaac tggcggtcat gtacttatga ctcctgaaga
      721 agaccgcttc ttaatgacta aaaaccaatt aattgctcgt atcgtcggtt atttaggcgg
      781 tcgtacaagt gaagaagtat tctttgatga tgtatcaaca ggcgcacaaa atgatattga
      841 agtcgctaca agaattgctc gactgatggt tactgaattt ggtatgagtg atttaggtcc
      901 gattcaatat gaaagagata caggcagtgt ctttttagga cgcgattaca cttcaagtca
      961 aaagaacttt agccaacaag ttgcgcttga aattgacaaa gaagtacgca agattattga
     1021 acaagcccat gataaagctc gggaaattat tgaaaaacat aaagatgatg ttattcttat
     1081 tgccgaaaca ttacttgacc gcgaaacaat tacggcagaa caaattgatc gtttgttaaa
     1141 agatcgtaaa ctcgatgaac caaaacaagc agtgattggt gaagttgttt taactgaaaa
     1201 accaaaagtt gaaccaacac caacacctgt tccggaagac gataaaaaat aagaaatcga
     1261 aagccgaagc aattcggctt tttaataaca atgtggaaaa taggaaacat agaaattaaa
     1321 ggtaaaacca ttcttgctcc aatggcgggc ataacgactt ttgcttatcg cgaatttatg
     1381 cgctccttcg gtgtagcgct ttgttatacc gaaatgataa gtgatagcgg ccttattttt
     1441 aataatgaaa aaacatatga atatttacca cgaccagaag aaaaaagacc aacatcggtg
     1501 caactttttg gcggtgaaac aactaagctt ttagtggcaa ttgaaaagat tgaaaagcac
     1561 acggataatt atgattttct tgatttaaat ttaggatgtc ccgtacctaa ggtaacacga
     1621 aataatggcg gttctgcatg gttaaagcat ccaaacgagc ttattgctat ggttgaaaaa
     1681 gttgttaaaa gaagcacaaa accagtgact gttaaaatta gaattgggtg ggatgaaaag
     1741 catattaatt tcaaagaact attaccaaaa ttagaagaag caggcgtgag tttaattgcc
     1801 attcacttgc gaacaacaaa acaactttat ggcggaaaag ctcgctacga tttagcgcaa
     1861 aatattcaaa atttagtaag tataccgctc gttgtaagcg gggatattta tacacttgat
     1921 gatgcgataa aggcgcttga aataactggc gcacaagcag tgatgctagc gcgcggtcaa
     1981 cttggaaatc cgtttttagc aacacagatt gagacatact ttaaaacggg agagagatta
     2041 aaagatccct cactatacca gcaattagat tatttgtgga cactggcaaa tatgatggtc
     2101 gaagaaaaag gagaacacat gggaatctta gtcatgcgcg gaatcgctca tcactttatt
     2161 aaagggtttc cgtttaccaa aacatacaaa gttaaactaa ctaaaatgtc gactttaaac
     2221 gacttagaat taataattaa ggagattaag aaccaggctt tgcttatata agcaaaaagt
     2281 ggtaaaatgt ccacatctaa aggagttatt tatgccaaga gaatttagtg atcaagaatt
     2341 agtgagacgc gctaaagcgg aagaacttag tgcgcaaggt atcgatccat ttggaaaaaa
     2401 gtatgaacga acagatacta gtctttcaat taaagaaaaa gctggtgaat ttactaaaga
     2461 agaattagaa gaaaaagcta ttaaagttaa aacagcggga cgcataatgc taatgcgtat
     2521 tatgggtaaa gctagttttt ttacgatcca agaccaagaa ggaagaattc aagcctttat
     2581 tagtaaacaa ggtgttggtg aagaagttac gacttattta aaagtgctga tattggtgac
     2641 atcgttggtg ttgaaggaac aattatgcgt accaaaacgg gcgaagtcac agtgaaggtt
     2701 gaagtgtata cccatattgt aaaagcatta cgaccacttc cggaaaagtt tcacggatta
     2761 actgatgttg aagagcgtta tcgtcgccgt tatgttgatt taataatgaa tgaatcatca
     2821 aaacatgttg ccttaactcg tccgaaaatt attcgtgcga ttcaaaaatt cttagatgat
     2881 cgtggatttg ttgaagttga aacaagtatg ttaaatagtg tacttggtgg cgcgagcgct
     2941 aaaccattta ttactcatca taatacgctt aatcgtgact tttacctgcg aattgccacg
     3001 gaattaccat taaaacgctt attagtcggt ggcattgaaa aagtatatga aattggtcgt
     3061 ctcttccgta acgaagggat gagcacgcgt cataatccag aatttacaac ggttgaagct
     3121 tatcaagctt acgctgattt agatgatatg tatgaacttt gcgaaagcct agtaagagaa
     3181 cttgcgatta aagtcacagg tgatactaaa ttaccatggg gcgatgaaat tattgacgtt
     3241 ggtgcgccgt tccgcaaagt atcaatggtt gatttgatca aagaaaagac ag
//
LOCUS       DAOSEX010000071         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_27349_length_3281_cov_2.814011, whole genome shotgun sequence.
ACCESSION   DAOSEX010000071 DAOSEX010000000
VERSION     DAOSEX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27349_length_3281_cov_2.814011"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /gene="nusA"
                     /locus_tag="PK612_01985"
     CDS             complement(<1..1364)
                     /gene="nusA"
                     /locus_tag="PK612_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOH94914.1"
                     /translation="MSKKLTKAQLRKKQERFETMREMFREVAEKHNLKEEVVLNVFMR
                     AVQNVVARQKGGDDVHVEVVINEEMFDFEARVLKRVVAHDDDIEDDALEVCLEVARTF
                     DADAEVDALVVVERSFLGDFNSKLSNNVAHNFRHQLIELEKEQLFTLFKDKIGELVKG
                     TVEKNERITYINLLGITSVFFDERGRHAIPGEVFKPGQELNFYIVDVVSGAKGANIII
                     SRSHPNFVKRLMEREIHEIYDGTIVIHDIVREEGERVKVAVESLDPNVDPIGACIGPN
                     ASRIQKITAELGPDGHKEKIDIIPWVKNEALFIIEAFRPAVPLGLKIDEENKTAQVVF
                     SEEEKGKAFGHKGANIRLASKLTGYDLTILDLNEASEQGVEFLTKADIEYEERLAEQE
                     RTRERILRELARAEEVAQRAANQKSLLEEEVNSLNIDDLEYEDAFKDEEKVVETPKVV
                     TPEI"
     gene            complement(1379..1834)
                     /locus_tag="PK612_01990"
     CDS             complement(1379..1834)
                     /locus_tag="PK612_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94915.1"
                     /translation="MDLNLLQPKVEELLKERGYRLVSMRFLNEEGTRFLRVIVDKYQH
                     IISLDEIVAISEAIDAILDDDEESAFTLDITTTGAEKEIALQEVNDYLGLFIKLTMKD
                     GVKGPNTVKGILEAVSENEVTISHNDKGRIKKETYAQSDIKTINRAVKY"
     gene            complement(2084..2794)
                     /locus_tag="PK612_01995"
     CDS             complement(2084..2794)
                     /locus_tag="PK612_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94916.1"
                     /translation="MLQHESKHYIFNYYAGSKAAEDILLIAEHQQACYTHICKVLGVE
                     PNFKIKYFLCNSPEEVGKIYGDNEPCNAFTSPPDSIYAVYNDDIQCIGFHEDAHIISY
                     TINRPESAAIREGLAMYFDRVWWGISNEDWTLYYLKTNKYISICNLLDNKYFFSEDCS
                     LTYPIMGAFTNYLIVHYGIKKYLVFYRYKDNIFDGFEKVYKLSVNELEMILKKIAKLE
                     LTNDTESKIKQLLMKNKT"
BASE COUNT          978 a          637 c          484 g         1182 t
ORIGIN      
        1 ttaatttcag gagtgacgac ttttggtgtt tcaacgactt tttcttcatc tttgaaagca
       61 tcttcatatt ctaagtcatc aatattaagg gaattaactt cctcttcaag caaactcttt
      121 tgattagcgg cgcgttgtgc aacttcttcg gcacgcgcaa gttcgcgtaa aatacgttcg
      181 cgtgtccgtt cttgttcagc gagccgttct tcgtattcaa tatccgcttt tgttaagaat
      241 tcaacacctt gttcactagc ttcatttaaa tcaagaatcg ttaggtcgta gccagttaat
      301 ttactcgcta aacgaatatt tgcgccttta tgaccgaagg ctttaccttt ttcttcttcg
      361 ctaaagacga cttgtgctgt tttattttct tcgtcaattt ttaagccaag cggcacagcc
      421 ggacggaagg cttcaataat gaagagcgct tcgtttttaa cccatgggat aatatcaatc
      481 ttttctttat ggccatcagg accaagttca gcagtaattt tttgaatgcg gcttgcattt
      541 ggaccaatac aggcgccaat tggatcaaca tttggatcaa gactttcaac tgcaactttg
      601 acgcgttcac cttcttcccg cacgatatca tgaataacga tagtaccatc ataaatttca
      661 tgaatttcgc gttccatgag cctttttacg aagttaggat ggctgcggga aataataata
      721 tttgcccctt tagcgccact aacgacgtca acaatataga agtttaattc ttggccaggt
      781 ttaaaaactt caccaggaat cgcgtgccgt cctctctcat caaagaacac acttgtaatt
      841 cctaataaat taatataagt aattcgttca tttttctcca cggtaccttt aacaagttca
      901 ccaattttat ctttaaataa agtgaagagt tgttcttttt caagttcaat taattgatga
      961 cggaagttat gggcgacatt atttgataat tttgaattga aatcgcctaa gaatgagcgt
     1021 tcaacgacga ctaatgcatc gacttctgca tcagcgtcaa atgtacgcgc tacttctaaa
     1081 catacttcaa gtgcatcatc ttcaatatcg tcatcatgag cgacaactct ttttaagacc
     1141 cgtgcttcaa aatcaaacat ttcttcgttg atcacgactt caacatgcac atcatctcca
     1201 cccttttggc gagcgacaac attttggaca gcacgcataa aaacattaag aacaacttct
     1261 tcctttaagt tgtgcttttc cgcaacttca cgaaacattt cacgcattgt ttcgaaacgt
     1321 tcttgctttt ttcttaattg tgcttttgtt aattttttac tcattttcgt attcctcttt
     1381 aatatttcac tgcgcgatta attgttttaa tatcactttg cgcataagtt tcctttttaa
     1441 ttcgtccttt atcgttatga ctaatcgtca cttcattttc actaacggct tctaggatac
     1501 cttttactgt gttaggacct ttgacaccat ctttcatcgt taatttaatg aataaaccta
     1561 gataatcatt tacttcttgt agcgctattt ctttttccgc gcccgtcgtt gtaatatcaa
     1621 gggtgaaggc actttcttca tcatcatcta gtattgcgtc aattgcttca ctgatggcga
     1681 caatttcatc aagactaata atgtgctggt acttatcaac gattacgcgt aagaaacgtg
     1741 taccttcttc attcaagaaa cgcatactta ctaagcgata accgcgttcc tttaatagtt
     1801 cttcgacttt tggttgtaat aagtttaaat ccataatcct cctgtaaaca aagagtgggc
     1861 gtacccactc tctagagttt gaattaattt gtaagtaaaa cttacagcat cataatagta
     1921 gattttgtac aactttgcaa ctaaaaagtg tcaaattact ataatattag cgattatttc
     1981 atatttacac cttaaattta caatgatttt ttcttaagaa gtacacgatc caataccgtc
     2041 gcaatagtaa aaaagactta cttaatcagt actgttttga ttttcatgtt ttgtttttca
     2101 ttaataattg tttaatttta ctttcagtat catttgttag ttcaagttta gcaatttttt
     2161 ttaatatcat ttctaattca tttacactta atttatatac tttttcaaaa ccatcaaaaa
     2221 tattatcttt atacctgtaa aagactaaat attttttaat tccgtagtgc actataagat
     2281 aattagtaaa agcacccata attggataag ttaatgaaca atcttcacta aaaaagtact
     2341 tattatcaag taaattacaa attgaaatat atttatttgt ttttaagtag tatagtgtcc
     2401 agtcttcatt acttatcccc caccatacac ggtcaaaata cattgctagt ccttcacgaa
     2461 ttgccgcgct ttcgggacga tttattgtgt aactaataat atgagcatct tcatgaaaac
     2521 caatacactg aatatcgtca ttatataccg catagattga gtcaggcgga ctagtaaaag
     2581 cgttacacgg ttcattgtca ccatatatct tcccaacttc ttcaggcgaa ttacataaaa
     2641 aatatttaat tttaaaatta ggttcgaccc ctaaaacttt acaaatatgc gtgtagcaag
     2701 cttgttgatg ctccgcgatt aaaaggatat cttctgcagc tttactaccc gcatagtaat
     2761 taaagatata gtgtttgctt tcatgttgta acattaagtt ttcgtttccc atttgtcgta
     2821 aacttttaag gcgtcctttc gcctcatctt attgctcata agttagtgct ttaattctta
     2881 gctaattata ctacgataaa cgcttcttac tcttatttag ttaacaatct cttaactaat
     2941 agtattattt aaactttatt aaataacatc actacttgac ttaatttgcg aaattaagga
     3001 aaataacgct ggtgatagcc gctgattacg cttattggga gctaatcact ccagagccct
     3061 tggactgttc aagggcattt tttaaattct aacactttcc aattattaat aacttatagt
     3121 aaattaaaat taattagtca tttaatttgt cattaattgg taattgaatt gacttaaatg
     3181 aaaaaaaata ggtaaattaa tattgggcat acgatgtaat gcgcgaatta agtttcgact
     3241 catctgtgtc tttttatttt gcttgaaata acaaataatc t
//
LOCUS       DAOSEX010000072         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_27542_length_3264_cov_3.664693, whole genome shotgun sequence.
ACCESSION   DAOSEX010000072 DAOSEX010000000
VERSION     DAOSEX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27542_length_3264_cov_3.664693"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1095)
                     /locus_tag="PK612_02000"
     CDS             complement(214..1095)
                     /locus_tag="PK612_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOH94917.1"
                     /translation="MKQNTNKVEFILDEYEYFEDLYIENGASDVRLVKTKRPNVRVVA
                     VNLSKYRVFVEKSILFVKEEHEYKYRAPFAAKKNNKRITIYLPENKYNDISIYSHYGA
                     KVVLPKTISFATIDASVAYGTLICSAPAKHLEVVHAESEIIIKDNKYETLYIDTTASL
                     TKLQNVKAKEITIYRAPFEFSEKVQFTNVSATKDIKIDVYNLELMFKNVIARQKLTIN
                     SDYYAVIKLNNIDATNIAMTLKKGSVSGTLSSAKEFDLIGTFKNNIKCPAFLVPNGTC
                     TIKIKEEGKVNLKVLEH"
     gene            complement(1204..2169)
                     /locus_tag="PK612_02005"
     CDS             complement(1204..2169)
                     /locus_tag="PK612_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOH94918.1"
                     /translation="MNKLEKILTIVASSLLVVGLVIFISVLAVNGFNLGGLSTVKYEA
                     VTHEVSEDYAKIQFDISVGDVTIVKGEEATTKAICYENKRLTYNLNVEEDTLVVTDNN
                     TMRRFWTIFDLWGNKGITLYVPHDTYTSLLVKNNTGTLNVSSSVTFNTITARLETGSI
                     TLNGDASVVDLSSETGSITLNNVDVDSARLVTATGRHVLNNVNVTGNLNIEVDTGTVK
                     LTNVKSDNLTVDSDTGTITLKDTVVTNTMRLAASTGSINFDKSDAHDIYARTSTGSIK
                     GSLLTGKVFSTKTNTGTISVPSNTAGAGVCDLRTDTGVIKITIAA"
     gene            complement(2166..2456)
                     /locus_tag="PK612_02010"
     CDS             complement(2166..2456)
                     /locus_tag="PK612_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94919.1"
                     /translation="MLIGAIAVFFSLVISLWAVELGLFVGSFEAFITVFTLLFAGEGL
                     KALAYFGISLAMAGLGILLLLVGVKLTKYTIELAKKLINKIKTKMVLKGAKK"
     gene            complement(2640..2768)
                     /locus_tag="PK612_02015"
     CDS             complement(2640..2768)
                     /locus_tag="PK612_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94920.1"
                     /translation="MTKKEFLSALREKMAGLPKDDVDDRIAFYSEMIDDRMEEGLS"
     gene            complement(2761..3081)
                     /locus_tag="PK612_02020"
     CDS             complement(2761..3081)
                     /locus_tag="PK612_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOH94921.1"
                     /translation="MDVQLKRGLLEVCVLAVIRNKPTYGYRIIKDMKPYVEISESTLY
                     TILKRLEENEMLTVKTVEHNSRLRKYYSITGRGLGRIAEFKEYWKDMEAMYKFIIKKE
                     SSDD"
BASE COUNT         1030 a          648 c          458 g         1128 t
ORIGIN      
        1 cagtgaacac cactagttta tttttgggga tattcacact aatgtttttt aagttgttat
       61 ccctagcgcc tttaataata atgtagtctt ttttatccat agctttcatt ttaccttatt
      121 cataaggtat ttagacaaat tatgcgcggt aagtaaaaaa ggggtaagac acaatgccgt
      181 taaccacttt aaaataaaag gcgaaaattt atattaatgt tcaagaactt ttaagtttac
      241 tttgccttct tctttaattt taatcgtaca tgttccattt ggtactaaaa atgcggggca
      301 tttaatatta tttttgaaag tacctattaa atcaaattct ttcgcacttg ataaggttcc
      361 actaacacta cctttcttta atgtcatagc aatatttgta gcgtcaatat tatttagttt
      421 aataaccgca taataatcac tattaattgt aagcttttgt ctagcaataa catttttaaa
      481 cattagttca agattataaa catctatttt tatatctttt gtggcgctaa cattagtaaa
      541 ctgaaccttt tcactaaatt caaatggtgc tctataaatt gtaatttcct ttgctttaac
      601 attttgtaat ttcgttaaag aagcagttgt atcaatataa agcgtttcat atttattatc
      661 tttaataatt atttcacttt cagcatggac aacttcaaga tgtttggcgg gggcactaca
      721 aataagtgtc ccgtaagcaa cactagcgtc tatcgttgca aaacttatag ttttaggaag
      781 tacaactttc gcgccataat gtgaatatat tgaaatatca ttatatttat tttcaggtaa
      841 gtaaatagta atccgcttat tattcttttt agcggcaaaa ggagctctat atttatattc
      901 atgctcttct ttaacaaaaa ggattgattt ctcgacaaaa acacgatatt ttgataaatt
      961 aaccgctact acccgcacat ttggtcgctt tgtttttaca agccgcacat cactagcgcc
     1021 gttttcaata taaagatctt caaaatattc atattcatcc aaaataaatt ctactttgtt
     1081 agtattttgt ttcattttaa gagactccta tttttctaat tttatcacgc ttaatgtaac
     1141 aatactttta aataagaaaa agtgcgttac ttctaacgca ctcactcggg ggacaaaatt
     1201 taactatgcc gcaatcgtaa tcttaatcac tccagtgtca gtgcgaagat cacaaactcc
     1261 ggcaccagcg gtattacttg gcacactaat ggtaccagta tttgtcttag tgctaaagac
     1321 tttaccagtt aagagtgaac ctttaatact acctgttgaa gttctagcgt aaatgtcatg
     1381 ggcatcactt ttatcaaagt taatggagcc cgtactagcg gctaaacgca ttgtatttgt
     1441 cacaactgta tcttttaaag taatcgtacc agtatcacta tcaactgtta aattatcact
     1501 tttaacattc gttaacttaa ctgtgccagt atcaacttca atatttaggt ttcctgttac
     1561 attaacatta tttaagacgt gtcttccagt tgccgtcact aatctagcgc tatctacgtc
     1621 aacattattt aaagtgattg agcctgtttc cgagctaaga tctacgacac ttgcatcacc
     1681 atttagtgtg atcgaccccg tttctaatcg tgccgtaatc gtgttaaaag ttacgctgct
     1741 tgaaacattt aatgtccccg tattattttt cactaaaagt gaagtataag tatcatgcgg
     1801 aacataaagc gtaatacctt tattacccca taaatcaaag atagtccaga aacgacgcat
     1861 cgtattattg tcagtaacaa caagcgtgtc ttcttcaacg ttaaggttat aagttaagcg
     1921 tttattttca taacaaatcg ctttagtcgt tgcttcttca cctttaacaa tcgtgacatc
     1981 cccaacactg atatcaaatt gaatctttgc ataatcttca ctaacttcat gagttacagc
     2041 ttcatatttc accgtactta atcctcctaa attaaagccg tttaccgcta aaacactaat
     2101 aaatatgact aggccaacta cgagcaagga actcgcaact atcgttaaaa tcttttctaa
     2161 cttattcatt tttttgctcc cttcaacacc atctttgttt taattttgtt aataagcttt
     2221 ttcgctaact caattgtgta cttcgttaac ttaacaccca ctagtaataa aaggatccct
     2281 agtcccgcca ttgcaagact tatcccgaaa taggctagtg ctttaagtcc ttcaccagca
     2341 aaaagcaaag taaagacagt aataaacgct tcaaatgagc cgacaaaaag accaagttct
     2401 acggcccaga gcgaaattac aagcgagaaa aacaccgcaa ttgccccgat aagcaaaccg
     2461 cctaatccaa taagaagcgg tacccaaagc ggtgcaagta caataattag aacaatttcc
     2521 catccttcta actttcgttt tggtgttact ttgtctttaa caatcttagt aattgatgtg
     2581 ccttcaattt gtttaatgac gatctcatca acattaccaa cttcggcgac cgcttctgct
     2641 taacttagtc cttcttccat gcggtcatca atcatttcgc tataaaaagc aatacgatcg
     2701 tccacatcgt cttttggtaa tcctgccatc ttttcacgca aggcacttag aaactccttt
     2761 ttagtcatca ctactctcct tcttaataat aaatttgtac atcgcttcca tgtctttcca
     2821 gtattcttta aattcagcaa tgcggccaag ccctctccct gtaatactgt aatatttccg
     2881 gaggcgagaa ttgtgttcaa ctgtcttaac agttaacatc tcattttctt cgagacgctt
     2941 taatattgtg tataatgtcg attcagatat ttcaacatat ggcttcatat ctttaataat
     3001 acggtagcca taagttggtt tattacgaat gacagcaagt acacaaactt ctaagagtcc
     3061 tcgttttagt tgtacatcca aagtcttata cctcccttaa cataatacat cgtaagccga
     3121 agtatataaa aagtcaattc atattattaa aaatttatat tttcttttaa attgaaaaaa
     3181 gagtagttat aaactactaa atttaaaaat gattaaggac attattaaga atcagtttca
     3241 atcagtgctt gtaattcaac atta
//
LOCUS       DAOSEX010000073         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_27637_length_3257_cov_3.162086, whole genome shotgun sequence.
ACCESSION   DAOSEX010000073 DAOSEX010000000
VERSION     DAOSEX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27637_length_3257_cov_3.162086"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PK612_02025"
     CDS             <1..1133
                     /locus_tag="PK612_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOH94922.1"
                     /translation="EIEPMMAVNLGTGTPKDAGNIVEYCNFPKGTYYSDLRIKNGHKE
                     PYNIKYWCLGNEMDGPWQICHLNAEDYAKKALETAKIMRWVDPSIKLIACGSSNAEIE
                     TYPDWDRIVMETLYDHVDYISMHRYYWYEDSLEDFFASYHDMDNFIHTIKSTADFVKA
                     KKRSDKVMKISFDEWNVWYLNKWKPGGWKKAPHILEDHYSLKDALVFSGLLNTLVNNV
                     DRVEIAALAQLVNVIAPIFTKEGGGVFKQTIFYPFYAVSNYGRGVALKAIKTGPKFNS
                     KFGEVNYVSEAVTFNEEKREISVFLVNYAQEKMEVELELRSFGRVEALTHKVMTHDDL
                     EIKNTFDEENVKFVEGELPTVKNDVATLELGPLSYHVYRFKY"
     gene            1146..2774
                     /locus_tag="PK612_02030"
     CDS             1146..2774
                     /locus_tag="PK612_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinan endo-1,5-alpha-L-arabinosidase"
                     /protein_id="HOH94923.1"
                     /translation="MKRYLFLISVAILALTACSGGKTITSISNSDNIEEAIIYPTKPY
                     SDSDHIMQKDSYAFYNIAGNDTRYWGAANTHDPAIIKEGDTYYAFSTDANFGVTSQKG
                     IHIRKSNDLIKWSFVGTALDVTTLEDQIEYIGYNRDGEKVDFFWAPDIIKRGNEFWLF
                     YSISGFGQRNSMMGLAKSNNIEGPYTFDKEILRTSQSVGGTPNAIDSAIFVEEVDGEE
                     KMYMSYGSWSAGIYIIELDPVTGYPLIAQTLVEREVTVNTAEKGVTATATKLVPSSSD
                     DPAFGTKILSITSSEAPYIIKENGYYYLFITNGVDLTYDYDVRVFRSRNILGPYVDGK
                     GVSALSATNYNSFRPYGNKVTIAHQFTYLTDDISYKRGWAGIGHSTAFKDEEQWFFAS
                     HYRGTYMDRHRFFLGIWKMHFVNDWPVIEPNRYTGEGSINLEQADISGNYRIQILHQS
                     AVNSMLTDDIISIVNRAEEITLTKQKAGEWYVVNGEYNGRYRLNNNQLELELNGVLYK
                     GVITPQFNYEFEKGVLGLSLINSDGTALWGNRII"
     gene            2787..>3257
                     /locus_tag="PK612_02035"
     CDS             2787..>3257
                     /locus_tag="PK612_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOH94924.1"
                     /translation="MYLGIEFGSTRIKGIVLNDNFEVIASGSYIWQSTLKDGYWTYDL
                     NEVWVGMNAVIDEIAKTINVKEIKSLGVSAMMHGFLPFDKNGKLLTPFRTWKNTNTTV
                     AATELSKQFAFNIPLRWSIAHLYQAYLDKESYVKDIDTFTTLAGYVHLTLTGKNV"
BASE COUNT         1071 a          498 c          667 g         1021 t
ORIGIN      
        1 cggaaattga accaatgatg gcagtcaatt taggaacagg cactccaaag gacgctggta
       61 acattgttga atattgtaat tttccaaaag gaacatatta cagtgattta cgaattaaaa
      121 atggacataa agaaccttat aatattaaat attggtgtct cggcaatgaa atggatgggc
      181 cgtggcaaat atgtcaccta aatgctgaag attatgctaa aaaagcatta gaaacagcaa
      241 agattatgcg ctgggttgat ccttctatta aattaattgc ctgtggcagt tcaaatgctg
      301 aaattgaaac atatcctgat tgggaccgta ttgtgatgga aacactttat gatcatgttg
      361 attatatttc catgcatcgt tattactggt acgaagatag tttagaagat ttctttgctt
      421 catatcatga tatggataat ttcattcata caattaaaag cactgctgat tttgtgaaag
      481 ctaaaaaacg ttctgataaa gtaatgaaga tttcttttga tgaatggaat gtatggtatt
      541 taaataaatg gaaaccgggt ggttggaaaa aagcaccgca tattcttgaa gaccattatt
      601 cacttaaaga tgcattagtc ttttcaggat tactaaatac gctagtaaat aatgttgacc
      661 gcgttgaaat tgctgcttta gcgcaacttg taaatgttat tgcccccatt ttcacaaaag
      721 aaggtggagg ggtgtttaaa caaacaattt tctatccgtt ttatgctgtg tcaaattatg
      781 gtcgtggtgt cgctttaaaa gcaattaaaa cgggacctaa atttaattca aagttcggtg
      841 aagtaaatta tgtttcagaa gcagtaacat ttaacgaaga gaaacgagaa atttctgtat
      901 tccttgtaaa ttatgcgcaa gagaagatgg aagttgaact tgaattacgt tcgtttggtc
      961 gtgtagaagc acttactcat aaagtaatga cgcatgacga cttagaaatt aaaaatacct
     1021 ttgatgaaga aaatgtcaaa tttgttgaag gtgagttacc aacagttaaa aatgatgttg
     1081 cgaccttgga attaggcccg ctttcctatc atgtctatcg ttttaagtac tagggaggta
     1141 ctagtttgaa gcgttatctt ttcttaatta gtgtggcgat ccttgcttta accgcttgta
     1201 gcgggggtaa aacaattacc tctatttcta atagtgacaa tattgaagaa gcaattattt
     1261 atcctactaa gccatatagt gatagtgatc acattatgca aaaagattct tacgccttct
     1321 ataacattgc gggtaacgac actcgctact ggggagcggc taacacgcat gatccagcaa
     1381 ttattaaaga aggtgataca tattacgctt tttcaacaga cgctaatttt ggtgtaacct
     1441 cacaaaaagg gattcatatc cgcaaatcaa atgatttaat taaatggagt tttgtaggaa
     1501 cggcgttaga tgttacaaca cttgaagatc aaattgaata tattggttat aacagagacg
     1561 gggaaaaggt tgatttcttt tgggcgccag acattattaa aagaggtaat gaattttggt
     1621 tattttattc aatcagtggt tttggacaac gcaattcaat gatgggatta gcaaagtcta
     1681 acaatattga aggaccatac acttttgata aagagatatt acgtacaagc caaagtgtag
     1741 gtggcacgcc aaatgcgatt gattccgcta tatttgtgga agaagttgat ggcgaagaaa
     1801 agatgtatat gagttatggc tcatggtcag caggcattta cattatcgaa cttgatcctg
     1861 tgacgggtta tccgcttatt gcccaaacac ttgtcgaacg cgaagtaaca gttaacactg
     1921 ctgaaaaagg tgtaacggca accgctacta aattagtgcc ctcctccagc gatgatcctg
     1981 cttttggtac gaaaatatta agtattacat ctagcgaagc gccttacatt attaaagaaa
     2041 atggttatta ttatttattt attacgaacg gtgttgattt aacttatgac tatgatgtgc
     2101 gtgtttttcg ctcacgcaat atcttaggcc cttatgttga tggtaagggt gttagtgcac
     2161 ttagtgcaac aaattataat tcatttagac cttatggcaa caaagtgaca attgcccatc
     2221 aatttacata tcttacagat gatattagtt ataaacgtgg ctgggcaggt attggtcact
     2281 caacggcatt taaagatgaa gagcagtggt tctttgcatc gcattaccgc ggcacttata
     2341 tggatcgtca tcgtttcttt cttggtattt ggaagatgca ttttgttaat gattggcccg
     2401 ttatagaacc taatcgttat actggtgaag gtagtattaa tttagaacaa gctgatataa
     2461 gcggaaatta tcgcattcaa atcctgcatc aatccgcggt taattcaatg cttactgatg
     2521 acattatttc gattgttaat cgcgctgaag agattacatt aactaagcaa aaagcaggtg
     2581 aatggtatgt tgtaaacgga gaatataacg gtcgttatcg ccttaataac aatcaacttg
     2641 aacttgaatt aaatggggtg ttatacaaag gagttattac cccgcaattt aattatgaat
     2701 ttgaaaaagg agtgctgggc ttatcactga ttaatagtga tggaacagca ttatggggca
     2761 accgtattat ttagaaagga agaaatgtgt atttaggaat tgaatttggc tcaacaagaa
     2821 ttaaagggat tgtcttaaat gataattttg aagtcattgc ttcaggatct tacatttggc
     2881 aatcaacact taaagatggg tactggactt atgatcttaa tgaagtttgg gtcgggatga
     2941 atgcagtaat tgacgaaatt gctaagacga ttaatgtaaa agaaatcaaa tcacttggtg
     3001 tgtcagcaat gatgcatggt tttttaccat ttgataaaaa cggaaaatta ttaacaccat
     3061 tccgcacatg gaaaaacacg aacacaacag tagcggccac ggagttatca aagcaatttg
     3121 catttaatat tccgttacga tggtcaattg cccatcttta tcaagcttat ttagataaag
     3181 aaagttatgt aaaagatatt gatactttta ctacattagc aggttatgtc catctaacgc
     3241 ttaccggtaa aaatgta
//
LOCUS       DAOSEX010000074         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_27799_length_3244_cov_2.910003, whole genome shotgun sequence.
ACCESSION   DAOSEX010000074 DAOSEX010000000
VERSION     DAOSEX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27799_length_3244_cov_2.910003"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK612_02040"
     CDS             <1..369
                     /locus_tag="PK612_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94925.1"
                     /translation="ALHIHDLADDAYFEGEEAGRAASLFINGKLKAPTNTLQVVPGYG
                     INYVIPNYLELPLNTDELVLKFRPKENYENKRIVVKIDDTVIFKKFFPFLFPSELVIL
                     KVKKELLVAGEKLIVEVSDE"
     gene            362..709
                     /locus_tag="PK612_02045"
     CDS             362..709
                     /locus_tag="PK612_02045"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOH94926.1"
                     /translation="MSSYKKVTCLVCPRGCIMTVSSDLKVSGNFCARGVPYALQEVKE
                     PKRNLTYVVQVEGREAPLPVRTDSMIAKHLIPDVVKLLRTVTIKPPVAFNAIIIANVF
                     DTGVNIISSAEIK"
     gene            792..2480
                     /locus_tag="PK612_02050"
     CDS             792..2480
                     /locus_tag="PK612_02050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOH94927.1"
                     /translation="MNDKVKIFALGGLDEHGKNMYVIEINNDIFVLEAGLKYPDKSSP
                     GIDFIIPNHEYLITNKSRIKAYFISHGHDDQFGALPFIYKQAPAPIYTTKAAAMMLER
                     FTRRVGVKDVKYEIFTIEPSSQHNIAGHKFTFFQTTHSAMHSFGIALDTSQGTIVYSG
                     DFIIEYTNSARYKLDLNSIAKIAEKNVLCLLSESSGAEKAGYTSPTHRLAPNIVPIFR
                     ETKGRIFISLYDQSTYNIEELVQVVINAKRKIAFYDPNTEEYFRDFAKAGLSLIDERY
                     IISNDDLLRARDQDIVILMLGAGEKIYEKIIALSNRENPDKKIVLGKDDTFIMALPAP
                     ATLEVIATEALDSLYTTGCNVVNITRKLFTNMHAQEEDLRMLLSLLKPKYYIPVKGLY
                     RQQIANAQLALHSGLKFSHHNIFLLDNGVSVEFNDGVGRVKMSPLDLIPSGDVLVDGT
                     GIGDINRGVIEDRQRLSEDGIIIMAIAVNYDERTITAGPDVQMRGFVYVKDSEQLLKQ
                     IRQIFINTVSEALDARQFDIDGAVKETESRVEKYIFRETRRHPMILPLIRVINE"
     gene            2556..>3244
                     /locus_tag="PK612_02055"
     CDS             2556..>3244
                     /locus_tag="PK612_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94928.1"
                     /translation="MSEAKTNLLMQQQRLSRGLKGIIVALLALIGILKISIGGVINVP
                     VALLFGDLFVVFFALQVLFAFYLIFFPKAKSKKRVTLVFLSIFGLLIISSAFYAGNLR
                     NYMRDGTTFKDNLDVMVTLARDTFSFTPPSNAYFYNYGGILGEGLLFVLKDLPVWLII
                     IILVLANIGFATMLLWPLLVKIYAAIKEEIRDLKKRRRVLHEAGQLHKEEAPTEEDQV
                     PVTEIMNDNEV"
BASE COUNT         1023 a          543 c          649 g         1029 t
ORIGIN      
        1 gcactccata tccatgactt agcggatgat gcttattttg aaggtgaaga agctggaaga
       61 gcggctagtt tatttattaa cggaaagtta aaagcgccca ctaacacgct acaagtagtg
      121 ccaggctacg gcatcaatta tgttattcct aattatttgg aactcccctt aaacactgat
      181 gaattagtgc ttaaatttag accaaaagaa aattatgaaa ataaaaggat tgttgtaaag
      241 attgatgata cggtgatatt taagaaattc ttcccgtttt tatttccgag cgaattagtg
      301 attttaaaag taaaaaaaga actattagtc gctggtgaaa aactcatcgt ggaggtaagt
      361 gatgagtagt tacaaaaaag taacttgtct tgtttgtccg cgcggttgta ttatgactgt
      421 gagcagcgac ttaaaagtaa gcggtaactt ttgtgcgcgt ggtgtccctt atgcgctcca
      481 agaagtaaaa gaacctaaac gcaatttaac ttatgttgtg caagttgaag ggcgtgaagc
      541 acctttaccc gtccgtactg actcgatgat tgcaaagcac ttaattcccg atgttgtcaa
      601 gttattacgc actgttacga ttaagccacc agttgcgttt aacgctatta ttattgccaa
      661 tgtctttgat actggtgtta atatcattag tagtgccgaa attaaataaa gtaattactt
      721 ggtagtttag ctaaactttt gttatactaa caaagtagag atatcacata ttattaacaa
      781 gaggtataat tatgaacgat aaagtaaaaa ttttcgcttt aggggggcta gatgaacacg
      841 gcaaaaatat gtacgttatt gaaattaata acgatatttt tgtgttagaa gctgggttaa
      901 agtatccaga taaatcttca cctggtattg attttattat tccgaatcac gaatacttaa
      961 ttacgaataa aagtcggatt aaagcttatt ttattagtca cggccacgat gatcaatttg
     1021 gtgcattacc gtttatctat aaacaagcac ccgcaccgat ttacacgaca aaagccgcgg
     1081 cgatgatgct tgaacgcttc actcgtcgtg ttggtgttaa agatgttaag tatgaaatat
     1141 ttacgattga accatcaagt cagcataata ttgctggtca taagtttact tttttccaaa
     1201 caacacactc ggcgatgcat agctttggga ttgcgctaga cactagtcaa ggcacgattg
     1261 tgtattctgg tgactttatt attgagtata cgaacagtgc acgctataaa cttgatctta
     1321 atagcattgc caaaattgcc gagaaaaatg tgttatgttt acttagtgaa tctagtggtg
     1381 ccgaaaaggc gggatatact tcgcctacac accgtttagc gccaaacatt gtaccaattt
     1441 ttcgtgaaac aaaggggcgt atctttattt cgctttatga tcaatcaact tataacatcg
     1501 aagaattagt gcaagttgtg attaacgcta aacggaaaat tgctttttac gatccaaata
     1561 ctgaagaata ctttagagat tttgcaaaag ctggtttaag cttaattgat gagcgttata
     1621 ttataagtaa tgatgactta ttacgcgcta gagaccaaga tatagttatt ttaatgcttg
     1681 gcgctggtga aaaaatctat gaaaagatta ttgccctttc caatagggaa aatcctgata
     1741 aaaagattgt acttggcaaa gatgacacct ttattatggc tttgcctgct ccagcgacac
     1801 ttgaagttat tgccaccgaa gcacttgact ccttatacac aactgggtgt aacgtcgtca
     1861 atattacacg caaattattt acgaatatgc atgcgcaaga agaagactta cggatgttac
     1921 tctcactttt aaaaccaaaa tattatattc ctgttaaagg tttataccgg caacaaatcg
     1981 ctaatgctca actcgcgctg catagtggct taaagtttag tcatcataat atattcttac
     2041 tagataacgg agtcagtgtt gaatttaatg atggcgtcgg gcgcgtgaag atgagtccac
     2101 ttgaccttat tcctagtggt gatgtgttag tggacgggac aggaatcggt gatattaatc
     2161 gcggggtcat tgaagaccgc caacgcttaa gtgaagacgg aattattatt atggcaattg
     2221 ccgttaatta tgatgaacgc acaattaccg caggccctga tgtgcagatg cgtggctttg
     2281 tgtatgttaa agatagtgaa caactattaa aacaaattcg gcaaatcttt attaatactg
     2341 taagtgaagc actagacgcg cgccaatttg atattgatgg cgcggtaaaa gaaactgaat
     2401 cacgtgttga aaagtatatt ttccgtgaaa caagacggca cccgatgatc ttaccgctga
     2461 ttcgggtaat taacgaataa caattaatca caaaaattta ttaatttttg ctctcttttt
     2521 cggatattca agtaatatta tgtataatat taagtatgag tgaagcaaaa acaaatttac
     2581 ttatgcaaca acagcgactt agccgtggct taaaaggcat tattgtcgcg cttttggcac
     2641 ttattgggat tttaaaaatt tcgattggtg gcgtcattaa tgtcccggtt gcacttttat
     2701 ttggcgactt atttgtcgtc ttttttgctt tacaagtatt atttgctttt tatttaatct
     2761 ttttcccaaa agctaaaagt aaaaagagag ttaccctagt gtttcttagt atttttggct
     2821 tattaattat tagtagcgcc ttttacgctg gtaatttacg caattatatg cgtgacggga
     2881 caacttttaa agataattta gatgtaatgg taacgcttgc ccgtgataca ttttctttta
     2941 cgccaccgtc aaatgcttac ttttataatt atggcggtat tttaggtgaa ggccttttat
     3001 ttgtacttaa agatttacca gtgtggctca ttattattat tttagtgtta gcaaatatcg
     3061 gttttgcgac aatgttactc tggccactgc ttgtgaaaat atatgcagcc attaaagaag
     3121 aaattcgtga cttaaagaaa agaagacgag tgctccacga agctggtcaa ttacataaag
     3181 aagaagcgcc cacagaagaa gaccaagtcc ccgttacgga aataatgaac gataacgaag
     3241 tatg
//
LOCUS       DAOSEX010000075         3228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_28011_length_3228_cov_3.581153, whole genome shotgun sequence.
ACCESSION   DAOSEX010000075 DAOSEX010000000
VERSION     DAOSEX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28011_length_3228_cov_3.581153"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PK612_02060"
     CDS             <1..260
                     /locus_tag="PK612_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOH94929.1"
                     /translation="CHAEFNAILNTRNGSALDGCTLYVTLFPCNECAKAVIQVGIKEI
                     VYLDDKYHNEVSMRASRHMLKLAGVKLTPFKGKVLDPKFNE"
     gene            253..1068
                     /locus_tag="PK612_02065"
     CDS             253..1068
                     /locus_tag="PK612_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006626590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOH94930.1"
                     /translation="MNKYALEIMNLNYSYDKNVEVLNGVTFNVKAGTYVSVIGHNGSG
                     KSTLAKLIAGILDVGKGSVIKVFGKPLSSYDDERPDIGIVFQNPDNQFIGATVRDDIA
                     FGLENKRVAHAEMDEIINEYAAKVKMSEFLDKEPSNLSGGQKQRVAIAGVLAMKPRIL
                     LLDEATAMLDPRGKREIKEIVTELRKEVPDLTIISITHDLEETLHSDEIIVMNEGKIV
                     LQGTPEDIYKNHETLTSIRLDLPFIFRIKLELEKQGIKVKSKDLESLAEELWP"
     gene            1059..2018
                     /locus_tag="PK612_02070"
     CDS             1059..2018
                     /locus_tag="PK612_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOH94931.1"
                     /translation="MAIKFHHIYFSYAPKSPFQYDALHDINLEIKQNSFTAVIGHTGS
                     GKSTLVQHINALLVPTSGAVKVEDFLILPCKKKKYFRQLNRKLKSKKVSAEQKQRLAF
                     LKSIVESGAAYKVKELRKKVGVVFQFPEYQLFEETVIKDVAFGPKNFGVSNEEALALA
                     KNALTLVGIDESFYSRSPFELSGGEKRRVAIAGILALKPEILVLDEPTAGLDPLSAHE
                     MMEVFANIHRAGTTIVLVTHDMDLVLRYASDVVVMKDGKIVEETTPQKLFADVKEEYS
                     LEVPAFYAFMQALVQRGCPLDITKINTVSDLAKMVKNLKEQAK"
     gene            2015..2899
                     /locus_tag="PK612_02075"
     CDS             2015..2899
                     /locus_tag="PK612_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOH94932.1"
                     /translation="MKNISLGRYMPADSFMHKADPRIKFFGLVVFMVLIFLKFANPAM
                     DFLMYGIYAVVVFILMKVTKLKIFSLFKQLKSLWFMMIFLLIINIFTFRNREGNILFM
                     IGNFAIKDAALYQTLYIFIRLVLMIALTSVLTASTRPLDLTYAIEWYMSPLKVIRFPV
                     HEIAMTLSIALRFIPTLLDETGRIMRAQESRGVDFKGGKVKEKLRGVVALIVPLFVSA
                     LQRSDELANAMIARGYDPQAKRTRYRILRFTWRDLIVLLLAVSFLTLVILLRVYHIDL
                     ANMLYVNIQNLLSLWGLK"
     gene            2899..>3228
                     /locus_tag="PK612_02080"
     CDS             2899..>3228
                     /locus_tag="PK612_02080"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HOH94933.1"
                     /translation="MRYVAIVKYLGTNYFGWQRQPFEISVQQVIEDKLSTIFNTEIVI
                     YASGRTDARVHAYGQVFHFDAPPFPVSKLKYALNKMLPGDIEIVKLQKAKDDFHARYA
                     AKSKTYEY"
BASE COUNT         1022 a          521 c          677 g         1008 t
ORIGIN      
        1 tttgccacgc cgagtttaac gccatcctca acactcgtaa cggttcggct ttagatggct
       61 gtactttgta cgtcaccctc tttccgtgca atgaatgcgc gaaagcggtc attcaagtcg
      121 ggatcaaaga aatcgtttat ttagatgata agtaccataa tgaagttagt atgcgcgcta
      181 gtcgccatat gcttaaatta gcaggagtga aattaacacc atttaaaggt aaagtgttag
      241 accccaaatt taatgaataa atatgcctta gaaataatga acttaaatta ctcttacgac
      301 aaaaatgtcg aagttttaaa cggcgtcact tttaatgtta aagcgggcac ttatgttagt
      361 gttatcggtc ataacggcag tgggaaatca acattagcga aactcattgc tggtatttta
      421 gacgtcggta aagggagtgt cattaaagta tttggtaagc ctttatctag ttatgatgat
      481 gagcgaccag atatcgggat tgtttttcaa aaccccgata accaatttat tggtgcaacg
      541 gtgcgtgatg atattgcttt tggtctcgaa aataaaagag tagcccatgc cgagatggat
      601 gagataatta atgaatacgc cgctaaagta aaaatgagtg agttcttaga taaagaacca
      661 agcaacttaa gtggtggaca aaagcaacgc gtggcgattg ctggtgtgtt agcgatgaaa
      721 ccacgcattt tactactaga tgaagcgacc gcgatgcttg atccacgtgg gaaacgcgag
      781 attaaagaaa ttgtgacaga gttaagaaaa gaagtaccag acttaacaat catcagtatt
      841 acgcatgact tagaagaaac attacatagt gatgaaatta ttgtgatgaa tgaaggcaaa
      901 attgtgctgc aaggtacacc ggaagacatt tataagaatc acgaaacatt aacgagtatt
      961 cgccttgatt taccctttat tttccggatt aaacttgaac ttgaaaaaca aggaattaaa
     1021 gttaagagta aagatttaga aagtcttgcg gaggaattat ggccataaag tttcaccata
     1081 tttattttag ttatgcgcca aagtctcctt ttcaatatga tgcattgcat gatattaatt
     1141 tagaaattaa gcaaaatagt tttacagcgg ttattggtca cactggtagt ggaaaatcaa
     1201 cgcttgttca acatattaat gcacttttag tcccaacttc tggtgccgtt aaagttgaag
     1261 attttcttat tttgccttgt aaaaagaaaa agtactttcg acaattaaac cgtaagctta
     1321 aaagtaaaaa agtaagtgcc gaacaaaagc aaagactagc gtttttaaaa agtattgttg
     1381 aaagtggcgc tgcttataaa gttaaagaat tacgaaaaaa agttggcgtt gtctttcagt
     1441 ttcctgaata tcaactattt gaagagaccg tcattaaaga tgttgctttt ggcccgaaaa
     1501 attttggggt gagtaatgaa gaagcactag cgttagcgaa aaacgcttta acattagtcg
     1561 ggattgatga atccttttat agtcgttccc catttgaact ttccggtggt gaaaaacgcc
     1621 gggtggcaat tgctggtatt ttagcgctta agcctgaaat tttagtgctt gatgaaccaa
     1681 ctgcgggtct agatccttta agcgcccatg aaatgatgga agttttcgct aatattcaca
     1741 gagcggggac aacaatcgtg ttagtcacac atgatatgga tttagtgctg cgttacgcta
     1801 gtgatgtggt ggtgatgaaa gacgggaaga ttgtcgaaga aacaacacca caaaaacttt
     1861 tcgctgatgt gaaagaagaa tatagtttag aagtaccagc attctatgct tttatgcaag
     1921 cgttagtgca gcgcggctgt ccacttgata ttactaaaat aaatactgta agtgatttag
     1981 caaaaatggt gaaaaacctt aaggagcaag caaagtgaaa aatatctcct tagggcgtta
     2041 tatgcctgct gattcattta tgcataaagc tgatccacgg attaaatttt ttggtttagt
     2101 cgtatttatg gttttaatct tcctaaagtt tgctaatcct gccatggact ttttgatgta
     2161 cgggatttac gctgttgttg tttttatttt aatgaaagta acaaaattaa agattttttc
     2221 actctttaaa caactaaaaa gcttgtggtt tatgatgatt ttcttactaa taattaatat
     2281 atttacattt agaaatcgtg aaggtaatat cttattcatg attggtaact ttgcgattaa
     2341 agatgcagcg ctttatcaaa cactatacat tttcattcgc cttgtgttaa tgattgcttt
     2401 aacatcggtt ttaaccgcaa gtacgagacc gctagactta acttatgcga ttgagtggta
     2461 tatgagtccg cttaaagtta ttcgttttcc agttcatgaa atagcgatga cactgtcaat
     2521 tgcactccgg tttattccga ctttacttga tgaaacggga cgaattatgc gagcgcaaga
     2581 aagtcgtggt gttgatttta aaggcgggaa ggtaaaagaa aaattacgcg gtgttgtcgc
     2641 acttattgtt ccgctttttg ttagtgccct ccaacgcagc gatgagctcg ctaatgcgat
     2701 gattgcgcgc gggtatgacc cacaggctaa aagaacgcgt tatcggattt tacggtttac
     2761 ttggcgtgat ttaattgttt tattactagc ggttagtttt ttaacgcttg ttattttact
     2821 acgtgtctat catattgatt tagcaaatat gctctatgtt aatatccaaa atttactatc
     2881 tctttggggg cttaagtaat gcgctacgtc gcaatagtaa agtacttagg cacaaattac
     2941 tttgggtggc aaaggcaacc ctttgaaatt agtgtccaac aagtaataga agataagtta
     3001 tcaacaattt ttaatacgga aattgtaatt tatgcttcag gtagaacgga cgctagagtt
     3061 catgcttacg ggcaagtgtt tcattttgac gctccgcctt ttcctgttag taaattaaag
     3121 tatgccttaa ataaaatgct acctggtgat attgaaattg ttaaattaca aaaagcaaaa
     3181 gatgactttc atgcgcggta tgccgctaaa agtaagacat atgaatat
//
LOCUS       DAOSEX010000076         3138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_29150_length_3138_cov_2.577684, whole genome shotgun sequence.
ACCESSION   DAOSEX010000076 DAOSEX010000000
VERSION     DAOSEX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29150_length_3138_cov_2.577684"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PK612_02085"
     CDS             <1..753
                     /locus_tag="PK612_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94934.1"
                     /translation="VESIPLNVNNVTLPTTLEGYEGVTVEWTSSNAALNTETGAVTHP
                     VSTLDLTLTATVKKGTDQTVRSWTVTVFGTNLESLSFLHTLDLEDADPSGSYGCSNTK
                     PKYTVEATNDMVDLGDPVTKWQLKNALIGGDNNDKRTGMYAIRTQSNNDQARSGRIEL
                     QDNVYDFNMLEFDAAIYGANRKGMVLMFEVSTDDGVTWTQLEREYTINHFDLVKYRVR
                     TGTTGNVRVAIVLKAGTGQRINIDNIRLIKEA"
     gene            758..2275
                     /locus_tag="PK612_02090"
     CDS             758..2275
                     /locus_tag="PK612_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH94935.1"
                     /translation="MRRLATIGLLVILALTGCGKRTSSEVSLTTSAPSLSDEPSIATP
                     LSIQVFKNDIRAYDVTVDVSHTINLEARVLPLDAPQEVVWSVVNSELATVSATGAVTG
                     VAPGETMINVHALNYPDVKTSLFLTVNKVAEQTGVGSGTSADDPLFKGYEGDGPLEVY
                     FLETYKIYADSLYIKKGNIDIVIDGGWKCDGELNRDFLNEKVTDGRIDMLIASHGDGD
                     HTQGIPYLVRDIPHISSFLDYGRAGGESSGYKQLIRTFGENEGSPYYTALDSVNKVNG
                     ATNRYYFTSEFYVDVLNTGAYSDTSSGNANSLALMFNYKEFKFFTAGDLTSASETSLL
                     RNETLPQVSLYKASHHGSHGSNSQALMDTLNPYTVAIAATRAGIYNQDPAPPSPTNTY
                     NLDGTKGHPAAAAIERIYKIPRISLNLNVFWNAPNGTMKFTTYGGANDLVMNGSTPLR
                     HYYDLTLTNGEPVWNEELQDFENKVTGEENTKFHETKIFTFRGYTEYLPAWVTAA"
     gene            complement(2347..>3138)
                     /locus_tag="PK612_02095"
     CDS             complement(2347..>3138)
                     /locus_tag="PK612_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOH94936.1"
                     /translation="FVCKSAENDYFGYLKKMLLTLHNLYMINHDNLPVHGAMVEITLK
                     SNIKKRVVIVGDSGAGKSETLEALRQIASDEIKDIKVVFDDMGTLSTNEDGKVLASGT
                     ETGAFVRLDDLEQGYAYEVMDRALFLNPSQVNARVVIPLSRYSFIVEKYPVDIFLYAN
                     NYEDKDGIKVFDKPEEALAVFRKGMRRAKGTTSEVGMTASYFANPFGPMQYRAKTDRL
                     LNKYFKALSDNKVILGELYTKLALPEEEIRGPANAALALFKLLQG"
BASE COUNT          971 a          618 c          613 g          936 t
ORIGIN      
        1 gttgagagca tcccgcttaa tgttaataat gttacactac ctactacttt agaaggctac
       61 gaaggtgtaa ctgttgaatg gacttcaagt aacgcagcac ttaacacgga aacaggtgct
      121 gttacgcatc cagtttcaac acttgacctc acattaacgg caacagtcaa aaaaggaact
      181 gaccagacag ttcgctcttg gactgtaacc gtctttggta caaacttaga aagcctttca
      241 ttcttacata cattagactt agaagacgct gatcctagcg gtagttatgg ttgtagtaat
      301 acaaaaccaa aatatactgt agaagccaca aatgacatgg ttgatttagg cgaccctgtt
      361 actaaatggc aacttaaaaa cgccttaatt ggcggcgata ataatgataa gcgaactgga
      421 atgtacgcta ttcgtaccca aagtaataat gaccaagctc gttctggacg gattgagtta
      481 caagataatg tctatgactt caatatgctt gaatttgacg ctgcaattta tggtgctaat
      541 cgtaaaggca tggtcttgat gtttgaagtt tcaacggatg atggtgtcac ttggacgcag
      601 cttgaacgcg aatacacaat caatcacttt gatttagtta agtatcgtgt acgtactggt
      661 acaacaggta atgttcgtgt tgccatcgtc ttaaaagctg gtactggtca acgcatcaac
      721 attgataata ttcgcttaat caaagaggct taagcttatg agacgtttag ccacaattgg
      781 tttattagta atcttagcgc ttaccgggtg cggtaagcgc acctcttcgg aggtgtcact
      841 cacaacaagt gcaccttcat taagtgatga gccttcaata gccacacctt taagcattca
      901 agtttttaaa aatgatatta gagcatatga tgtcacagtt gatgttagtc acacgattaa
      961 tttggaggct agagtattac cgctagatgc cccgcaagaa gttgtgtgga gtgtcgttaa
     1021 tagtgaacta gcaaccgtga gcgctacagg tgcggtaact ggtgtagcgc ctggcgaaac
     1081 aatgattaat gtgcacgcgc ttaattatcc tgatgttaaa actagtttgt ttttaactgt
     1141 taataaagtt gcagagcaaa caggtgtcgg aagtggtaca agcgctgatg atccactttt
     1201 taagggatat gaaggcgacg gcccacttga agtttatttc cttgaaacat ataaaatata
     1261 cgctgattcg ctttatatta aaaaaggcaa cattgatatt gtgattgatg gtggctggaa
     1321 gtgtgacggt gaacttaatc gtgacttctt aaatgaaaag gtaactgacg gaaggattga
     1381 tatgcttatt gctagtcatg gcgacggtga ccatacacaa ggtattcctt atcttgtgcg
     1441 cgacataccg catattagta gcttccttga ttacggcaga gctggcggcg aaagcagtgg
     1501 ctataaacag ttaatccgca cttttggtga aaacgaaggt tcgccatatt acactgctct
     1561 agattccgtg aataaagtaa acggtgcgac taatcgctat tacttcacca gtgaatttta
     1621 tgttgatgtt ttaaataccg gagcatatag tgacactagc agcgggaacg cgaatagtct
     1681 agcattaatg tttaattata aagaatttaa gttctttacc gctggtgact taacttccgc
     1741 gagtgaaact agccttttac gaaacgaaac attaccacaa gtaagtttat acaaagctag
     1801 tcaccacggg agtcatggta gtaacagtca agccttaatg gatacattaa acccctatac
     1861 tgttgcgatt gctgctacta gagctggtat atacaatcaa gatcctgctc caccaagtcc
     1921 tactaataca tataatttag atggcactaa gggtcacccc gcagcagcag cgattgaacg
     1981 catttataaa attccgcgca tttctttaaa cttaaatgtc ttttggaatg ctcctaacgg
     2041 aacaatgaaa tttacaacat atggcggcgc taatgactta gtgatgaatg gtagtacccc
     2101 acttcgccat tattatgact taacactcac aaacggtgaa cctgtatgga acgaagaact
     2161 ccaagacttt gaaaataaag taacaggtga agaaaacact aagttccatg aaacgaaaat
     2221 ctttacattt agaggttata ccgagtactt accagcgtgg gttaccgctg cttaaataat
     2281 taaaatacag caaaaaagtc aggagcgatt cctgactttt ctttatgaat agttaaaacg
     2341 atcttactac ccttgtagta atttaaatag cgctagcgca gcgttcgcgg gtcctctaat
     2401 ttcctcttca ggaagcgcta atttagtgta gagttcccct aaaattactt tattgtcact
     2461 aagcgcttta aagtatttat taagtaatcg atctgtctta gcgcgatatt gcattggacc
     2521 aaacggatta gcgaagtaag aagcagtcat tccgacttca ctcgtcgtac ctttagctct
     2581 tctcattcct ttacggaaga cggcaagtgc ctcttcaggt ttatcaaata ctttaatccc
     2641 gtctttatct tcatagttat tagcgtataa gaatatatca actggatact tttctactat
     2701 aaaagaatat cgacttaaag gaataacgac tctagcgtta acttgcgaag gattaagaaa
     2761 gagcgctcga tccatcactt cgtaagcata gccttgttct aagtcgtcaa gtctaacaaa
     2821 agcacctgtt tctgtcccac ttgcaagtac tttaccatct tcgttagtgc ttagtgtccc
     2881 catatcatca aacacgactt taatgtcttt aatttcgtca cttgcaattt gccgtaatgc
     2941 ttctaaagtt tcacttttcc cagcaccact atcaccaaca ataactactc tttttttaat
     3001 gtttgatttt aaagtaatct ctaccattgc accatgtact ggtaaattat catgattaat
     3061 catataaaga ttatgtaatg taagtagcat ctttttaaga taaccaaaat aatcattttc
     3121 agcactttta cagacaaa
//
LOCUS       DAOSEX010000077         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_29788_length_3092_cov_2.967073, whole genome shotgun sequence.
ACCESSION   DAOSEX010000077 DAOSEX010000000
VERSION     DAOSEX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29788_length_3092_cov_2.967073"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PK612_02100"
     CDS             complement(<1..240)
                     /locus_tag="PK612_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94937.1"
                     /translation="MKKKAITLVVLLAMLVGCSRNESSSAPSVSSEGEVSTSEVNSET
                     TSEESSVASSEELPSSEELSSEEPSSEEPPSSSENP"
     gene            complement(481..>1136)
                     /locus_tag="PK612_02105"
     CDS             complement(481..>1136)
                     /locus_tag="PK612_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOH94938.1"
                     /translation="EDSSSIASSEESSVSVSSEPGVPYNVTFVTNCETMLDAIYTDVI
                     YDMPLIFREGYTLAGWYLEATFINIVSFPYYVTSDVTLYAKWTEGETAHFTFELHPNN
                     NGYIVTSYGGNAINVVVPTTYNDKPIVALGEKLFYQNGSIKSVVLPATLTYIGMQAFK
                     DAVSLESIVIPSNVTELATDAFAGTISLAQVTLSSKLKIIGNNAFENSAFAEVTILV"
     assembly_gap    1137..1236
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1237..1448)
                     /locus_tag="PK612_02110"
     CDS             complement(<1237..1448)
                     /locus_tag="PK612_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94939.1"
                     /translation="MRKRNSIKLLPVLASLLISGCRTELSSSEKSISEGTSISSEIVS
                     SETTSVTTSSEEVSATSEKSSSENGE"
     gene            complement(1477..>3092)
                     /locus_tag="PK612_02115"
     CDS             complement(1477..>3092)
                     /locus_tag="PK612_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH94940.1"
                     /translation="GASGASATSPKSFTNIQGITFGVSSNASKGAGTVKVYLKETATS
                     TTKTEVGSFTISSTGGTTRRESPTIVPSINNSGFIQFEVICSTNSVYIHDIKIDYQEV
                     VPDPVAVTGVTINPKTLSLTVGENSQATFTIAPSNATNKEVTFSSLNTSVATVTSDGL
                     ITAVSEGSTTIRVTTTDGNKTDDLSLTVSPAPVYEPYLNVDFSAFKKNYIFGEALALS
                     SIMAEHRASNGTITNLTGTDLTLVSGSTKELGVVPLNFSYDGLTASVNITVTNVGSYT
                     GAVAKDPLTIKGDSFAPAISTTTGTNYSSLSASGYTFAGVGLRNLDSYIMVYTSGHFR
                     NVSALETIKSLKLDYKTGGSSASVQAFRFGTTEITSASGAAHTTLTSSAGGTSATVNA
                     PAGTTHFRIDVSSKNLQANITITFESGSTYTFTAAEQAAAYEDYFMAVTHEECLVTDV
                     KLTTWNHLKTEFDAMLPEAQNLIRTTASVEFNARYNIIVAGYGYTNFLVSGGTGLGRD
                     LKNFENKLPILIMGLGLPLSVCFYIALKK"
BASE COUNT          888 a          602 c          499 g         1003 t
ORIGIN      
        1 cggattttca ctgctactag gcggttcttc actcgaaggt tcttcgcttg aaagttcctc
       61 actacttggc agctcttcgc tactcgcaac acttgattct tcacttgtcg tttcactatt
      121 aacttcacta gtggacactt ctccttcact gcttacagaa ggtgcgctac ttgattcatt
      181 acgcgaacag cccactaaca ttgctaatag tactacaagt gtaattgctt tctttttcat
      241 acttttttac ctctttattt ttagaccttt ctatttattt aaccacaatt tgtgcggaat
      301 atgcttgcca ataagggcta tttatatatg agttatatgc tgaggaaggc acataatatt
      361 tcatcgtcga aacagtgcct tcaaatgaat tagcgaaacg taatggtggt gtggttgata
      421 ttaaattaac acttagtaaa ttagtgttac cagcaaaagc acgtgaacta atttcagtaa
      481 ctaaactaga atagtcactt ctgcaaaagc gctattttca aaagcgttat tcccaataat
      541 ttttaatttg ctcgaaagtg ttacttgcgc taggcttatt gttccggcaa aagcatccgt
      601 tgctaactca gtaacattgc ttggaataac aatactttct aaactaactg catctttaaa
      661 agcttgcatc ccaatataag ttaatgtggc aggtaaaacg acagacttaa tactaccatt
      721 ttgatagaaa agcttttcac ctagtgccac gataggttta tcattataag ttgtgggcac
      781 aacaacatta atcgcattac caccgtaaga tgtaacaata taaccattat tattaggatg
      841 taattcaaag gtaaagtgtg ctgtttcacc ttcggtccat ttagcgtaca atgtcacatc
      901 actagtcaca taataaggaa aactgacgat attaataaat gttgcttcta agtaccatcc
      961 tgcaagagtg tagccttctc taaaaataag aggcatatca taaattacat cggtgtagat
     1021 tgcatcaagc attgtttcac aatttgtaac aaatgtaaca ttatagggca ctcctggttc
     1081 gctgcttaca gaaacagaag attcttcact acttgcgatg cttgaagagt cttcgcnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntctt cgccgttttc actagacgac
     1261 ttttctgaag ttgcgcttac ttcttcgcta gaagtcgtca cacttgtagt ttcacttgaa
     1321 acaatttcag aactaatact tgttccttcg cttatacttt tttcgctact acttaactca
     1381 gttcgacaac cactaataag tagtgacgct aaaacaggta ataattttat agaatttctt
     1441 tttctcataa ccttacttaa attataatgt tttttttcat tttttaagtg caatataaaa
     1501 acaaactgat aatggtaatc ctaatcccat tattagaatt ggtagcttgt tttcgaaatt
     1561 tttaagatcg cgacctagtc ccgtaccacc actaactaag aaattagtgt acccataacc
     1621 tgcaacaata atgttataac gagcattaaa ctccacgcta gcggtcgttc taattaaatt
     1681 ttgagcttca ggtagcatcg catcaaattc agtttttaaa tgattccatg ttgtaagttt
     1741 tacatcagtg acaagacact cttcgtgagt tacagccatg aaataatctt cgtatgccgc
     1801 tgcttgttct gcagctgtaa atgtgtatgt actacctgat tcaaaggtaa tcgttatatt
     1861 tgcttgtaag tttttgctac ttacatcaat tctaaagtgt gttgtacccg ctggagcgtt
     1921 taccgttgcg cttgtgcctc cagctgatga agttaaagtc gtgtgtgctg caccacttgc
     1981 acttgtgatt tcagttgtac caaatctaaa agcttgtact gaagcactac ttccaccagt
     2041 tttatagtct aatttaagtg atttaatcgt ttcaagtgct gaaacattgc ggaagtgacc
     2101 actagtgtag accataatat acgaatctaa attacgtaat ccaacaccag caaaagtata
     2161 accggatgct gataaactac tataatttgt gcccgttgtt gttgaaattg ctggagcaaa
     2221 tgaatcacct ttaattgtta gcggatcttt tgcaacggct ccagtataac ttccgacatt
     2281 agtcacagtt atattaacac ttgcagtaag gccatcataa ctaaagttaa gtggcacaac
     2341 acctaattct ttagtggaac cactaacaag tgttaaatct gtacctgtta agtttgtaat
     2401 cgtgccatta cttgcgcgat gttctgccat aattgaacta agcgctaaag cttcaccgaa
     2461 aatataattc tttttgaagg cactaaaatc aacatttaaa tatggttcat aaactggtgc
     2521 tggtgaaact gttaaactta agtcatcagt cttattacca tctgtagtag taacgcgaat
     2581 tgtcgtactc ccttcgctta cagcagttat tagaccatca ctcgtaacag tagcaacact
     2641 agtgtttaag cttgaaaatg ttacttcttt attcgtagca ttacttggtg caatcgtaaa
     2701 tgttgcttga ctattttcgc caacagttaa tgacaatgtt tttggattaa tcgtcacacc
     2761 agtgacagcg actggatcag gtactacttc ttgataatca atcttaatat catgaatata
     2821 cacgctattt gtcgaacaaa tcacttcgaa ttgaataaaa ccgctattat ttatacttgg
     2881 gacaatagtt ggactttcac gcctagttgt tccgccagta ctacttatcg taaaactacc
     2941 tacttctgtt tttgtagtgg aagtagctgt ttcttttaaa tacactttaa cagtacctgc
     3001 gcctttgcta gcgttagacg acactccaaa agtaataccc tgaatatttg taaagctttt
     3061 tggtgatgtc gcgcttgctc cactagcgcc tt
//
LOCUS       DAOSEX010000078         3087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_29857_length_3087_cov_3.077507, whole genome shotgun sequence.
ACCESSION   DAOSEX010000078 DAOSEX010000000
VERSION     DAOSEX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29857_length_3087_cov_3.077507"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PK612_02120"
     CDS             <1..696
                     /locus_tag="PK612_02120"
                     /inference="COORDINATES: protein motif:HMM:NF015159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOH94941.1"
                     /translation="RGCIHEEILNAAKTPLKEMGYRLNLETIDNALLANSSLSSGGID
                     ANIIQRTDDLIAYNNRVSKRAELTSAGRVYLSALGVYSGTKTTLEDYNPGDKIVISDR
                     AEDYERIMSFIEEVFTVTVFTEQEKTPNYRQTPLDFLTIEYMNPDLIPEAQKHPEEAF
                     YIMTGSQAYNSGLSYEHILATETSNDYAFIVGIRRGEENNDRIRALMKVLYSDEIGDF
                     IVEHFRGLIVPYR"
     gene            complement(698..1372)
                     /locus_tag="PK612_02125"
     CDS             complement(698..1372)
                     /locus_tag="PK612_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOH94942.1"
                     /translation="MSPLEIVLVSVLGALILIVAIYLFVVHTNAVNRLEIFEEVNKEA
                     KSGKIAFLGDSLTDFFPIQDFFPGVKIYNRGIAGDTTKNVLKRLDNVVALAPRKLFLQ
                     IGTNDLAKGSSPRKIVANIKKIITTFKKEVPNIEVICISLYPVSRTKKFLSTFIVGLR
                     TNKKIKKTNELLINFCAEEGLKYLDFYTLLADKKGRIDRRYTVEGLHISGKGYAVIAK
                     ALAPYL"
     gene            2078..3052
                     /locus_tag="PK612_02130"
     CDS             2078..3052
                     /locus_tag="PK612_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94943.1"
                     /translation="MKNAICRIGVLMAIAMGSLELVSMRIKEEPFDTSNTITRYTRET
                     SSTIRDSFFTTIDFKAAISDNTIIKGATLTADDSDMISLLNNDLYGIGYISYAALESS
                     ELKALIIEGVRVSEKSIIDDSYKLSRNFNYITKAPSDLSEKEYALITGFIAFSKSREG
                     LSIIKAKDGIVTQNIEEAESFTTLLAKSENSALAKMLYNTNEAPIHIKLGGSKSVESI
                     SRALSSAFTSLVPAFKAVHNYTSNEMAYEATQGSNKDSAEPLHIGFLARDLDINETEP
                     ALAGSYGVISKDAIVAVVSNKNHVLSDITRDQLKQIYKSETITWSALE"
BASE COUNT         1018 a          542 c          514 g         1013 t
ORIGIN      
        1 agaggttgca ttcatgaaga gattttaaac gcggctaaaa ctccacttaa ggaaatgggt
       61 tatcgcttaa atcttgagac aattgataat gctttgctcg ctaattcttc attatcaagt
      121 ggcgggattg acgcaaatat tattcaaaga acagatgatt taattgccta taataaccgg
      181 gtatcaaaac gcgcagagct tactagtgct ggtcgcgtct atctttcagc acttggtgta
      241 tattcaggaa cgaaaacaac actcgaagat tataatcctg gcgacaaaat tgtcattagt
      301 gatcgcgctg aagattatga gcggattatg agtttcatcg aggaagtttt tactgttact
      361 gtatttaccg aacaagaaaa aacaccaaat tacagacaaa caccacttga ctttttgacc
      421 attgaatata tgaatccaga tttaattccg gaagcccaaa agcatcccga agaagccttt
      481 tacattatga ctggttcaca agcttataac agcggactta gttatgaaca tattctcgct
      541 actgaaacta gtaatgatta tgcatttatt gtcggtattc gtcgcggcga agaaaataat
      601 gatagaatac gcgcactaat gaaagtatta tatagtgatg aaatcggtga ttttatcgtt
      661 gaacatttcc gcggattaat tgtgccttat cgttaattta taaataaggg gctaaggcct
      721 tagcaattac cgcataaccc ttccctgaaa tatgcaaccc ttcaactgta taacggcggt
      781 caatccgccc ttttttatcc gctaaaagag tgtagaaatc taaatattta agtccttctt
      841 cggcacaaaa attaattaag agttcatttg tctttttaat tttcttattc gttcttaatc
      901 caacaataaa tgtggataaa aactttttag tgcgtgatac gggataaagt gaaatgcaaa
      961 ttacttcaat atttggtact tcttttttga atgttgtaat aatctttttt atattagcga
     1021 ctattttacg aggactactt cctttggcta agtcattcgt gccaatttgt aaaaataact
     1081 tgcgcggcgc tagtgctact acattatcaa gcctttttaa tacatttttg gtcgtatcac
     1141 cagcaatacc gcggttatat atctttacac ccggaaagaa atcttgaatc ggaaaaaagt
     1201 ctgttaaact atcacctaaa aaagcaatct ttccgctttt ggcttctttg ttaacttctt
     1261 caaatatttc taagcgatta actgcatttg tgtgtacgac aaataaataa atggcaacaa
     1321 ttaaaataag tgcgcctaag acacttacta acacaatttc tagtggactc ataataagta
     1381 gctcctcttt tcaattatct ttattttagc aaatataata taagactttt tataaattaa
     1441 agactttttt actttttgat tataatctct tccacttcaa acattataaa actatttttg
     1501 tactttgttt atgccttaag agcacaaatg gttttcacat aaaattatgc gttacacgat
     1561 tgttttaaaa agtaattact atattttgtg gtgttttgtt gtttactttt gtggtatttt
     1621 gccagttata tttgtggtaa aaaatgcagc taataaataa agtatcgtaa ctgtacaagt
     1681 atcttattac ttataatcaa caattaacat atcatctgca aagtaaacat taaatataca
     1741 tgcgcgacga tcaggtgaag gtttatcaat aggagtatga actgaatcaa aaaatagatt
     1801 tcgctttatt gttttttata tctttaacta ttaatttgtc acttttttgt gagaaaggca
     1861 cactaaaaac aaacctaaat taactgtaaa attaaaaatt ttgcgggaaa atttaagttt
     1921 tttagtaata aaactattac tttaatgata ttttaaccaa aagcatcagc gaaaaactac
     1981 cacttttaca acttctttac aactttttac agctttatga tatatctagc tctccgtttg
     2041 ctttaaagtt tatgtgtctg aaaggaggtg ttcagatatg aaaaatgcaa tttgcagaat
     2101 cggagtactt atggcgattg ctatgggtag tctcgagctt gtatccatgc ggattaagga
     2161 agagccattt gatacaagta acactattac ccgttataca cgggaaactt cttccaccat
     2221 acgtgacagt ttttttacca cgattgattt taaagccgct attagtgata acactattat
     2281 taaaggagct acactaactg cggatgatag cgatatgatc tctttactca ataacgattt
     2341 atacggtatt ggatatattt cctacgccgc acttgaatct tccgagctta aagctttaat
     2401 aattgaaggg gttcgcgtct ccgaaaaatc aattattgat gattcgtaca aattatcacg
     2461 aaactttaac tacatcacaa aagctcctag tgacttaagt gaaaaagaat atgccctaat
     2521 tacagggttc atcgccttta gtaagtcacg cgaaggctta agtattatta aggccaaaga
     2581 cggaatcgtt acccaaaata ttgaagaggc tgaaagtttt acaacactct tagcgaaaag
     2641 cgaaaatagc gctctagcga aaatgcttta taacactaat gaagcaccaa tccacattaa
     2701 acttggtggt tcaaaatctg tcgaaagtat tagtcgtgcc ttaagtagtg cttttacttc
     2761 tttagtgccc gcctttaaag cagttcataa ctacactagt aatgaaatgg cttatgaagc
     2821 aacacaaggg agtaataaag atagcgctga accacttcat attggttttt tagcccggga
     2881 tttagatatt aatgaaactg aacccgcact tgctggtagc tacggcgtaa ttagtaagga
     2941 cgctatcgtt gctgtcgtca gcaataaaaa ccatgtcctt agtgacatca cacgcgatca
     3001 attaaaacaa atttacaaat ctgaaacaat tacgtggtcc gcacttgaat agcgcatcac
     3061 ttgttatcta actgtcacga taacatt
//
LOCUS       DAOSEX010000079         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_30420_length_3045_cov_4.095987, whole genome shotgun sequence.
ACCESSION   DAOSEX010000079 DAOSEX010000000
VERSION     DAOSEX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30420_length_3045_cov_4.095987"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PK612_02135"
     CDS             <1..1015
                     /locus_tag="PK612_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH94944.1"
                     /translation="IAYRTNRARASSSIFMAGNMSSPEAKEAILETLAENNITKKTYD
                     SFGFDYLGLKTIGSNAIVTFQARLDFEISKLNSDDYPTIDAYFAAVKAIKEELQASIT
                     ESLLTKLPAALSESMVGMQEYDVYIMTVGNMYFKIVGLLISVVFVIVVGVNLIAGQVD
                     SGSMAYILSTGTTRNSVTITQMIFFVSSTLFLYLTTSIVSVVCFFIAPPASTSVTLGT
                     LLLFNLGAFLVTMALGGIIYLASCLFNRSKRAMAIGGGFAVLTLVATILGLFASETTP
                     TMMRMSALDPFNYVSLVSLYDISAIVDGNFAFIWKFAILGIVAVICFALGIVVFKKKD
                     LPL"
     gene            complement(1030..1644)
                     /locus_tag="PK612_02140"
     CDS             complement(1030..1644)
                     /locus_tag="PK612_02140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HOH94945.1"
                     /translation="MDNIERKNKGLAYIVDKPILEEMKRCRKLIQELNTADWTDREKL
                     SKITKMLLGKSDSTMITPPFYCDYGSNIEVGKNFMTNYNCTILDVAKVTFGDNCMLGP
                     NVAIYTAGHPLHPKTRNSGYEYGKPIVVGDNVWIGGNSVVCPGVHIGSNVVIGAGSVV
                     TKDIPDWSLAVGNPCKVIRLITEDDIRKLFKNEEIDDDVWKIIG"
     gene            2250..2495
                     /locus_tag="PK612_02145"
                     /pseudo
     CDS             2250..2495
                     /locus_tag="PK612_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
BASE COUNT          910 a          584 c          585 g          966 t
ORIGIN      
        1 aattgcctat cgcacgaacc gggcccgcgc gtcatcttcg atctttatgg ccggcaatat
       61 gtcatcaccc gaagcaaaag aagccatcct tgaaacgctt gccgaaaata acataaccaa
      121 aaaaacctat gatagttttg gctttgatta tcttggttta aaaacgattg gaagtaacgc
      181 aattgtgacc tttcaagcgc gccttgactt tgaaatatca aaactaaata gcgatgatta
      241 tccgacaatt gacgcctatt ttgcggcagt taaagccatc aaagaagaac tacaagcctc
      301 aataacggaa tcgctattaa ctaaacttcc ggcagcgtta tccgaatcaa tggtggggat
      361 gcaagaatac gatgtctata ttatgacagt cggtaacatg tactttaaga ttgttggact
      421 tttgatctcc gttgtctttg ttatcgtggt cggcgttaat ctgattgccg ggcaagtcga
      481 ttcgggatca atggcctata tcctttcaac cggaacaacc cgtaacagtg taaccattac
      541 ccaaatgatt ttctttgtga gttcgacctt attcctatat ctaacaacct caatcgtttc
      601 ggtggtgtgc ttttttatcg ctccaccggc ttcgacaagt gtcacacttg gcaccttact
      661 cttatttaat cttggggcct tcttagtcac catggcgctt gggggaatca tctacttagc
      721 ctcttgtctc tttaaccgct cgaaacgggc aatggcgata ggcggtggtt ttgccgtcct
      781 aactttagtt gccactatct taggattgtt tgcgagtgag acgaccccaa ccatgatgcg
      841 gatgtcggct ttagatccat ttaattatgt atcacttgta agtctttatg atatcagcgc
      901 gattgtcgac ggaaactttg ctttcatatg gaaatttgca atccttggga ttgtcgccgt
      961 tatttgcttt gcacttggaa ttgtcgtctt caaaaagaaa gatttaccac tttaagaatc
     1021 gtaaagtatt tagccaataa tcttccaaac atcgtcatca atttcttcgt ttttaaataa
     1081 ttttcgtata tcgtcttcag tgataagacg gatgacttta caaggatttc ccaccgctaa
     1141 ggaccagtcg ggaatgtctt ttgtgacgac actgcctgcg ccgataacaa cattgctacc
     1201 aatatgaacc ccgggacaaa cgacagagtt accgccaatc cacacattat cgccaaccac
     1261 aattggtttt ccgtattcgt aacctgaatt tctggttttt ggatgaaggg gatgacccgc
     1321 tgtatagatt gcgacattcg gtccaagcat acagttatca ccaaatgtta cttttgcgac
     1381 atctaaaatg gtgcaattat aattcgtcat aaagtttttt ccaacttcaa tattagagcc
     1441 atagtcacaa taaaaaggtg gtgtaatcat cgtcgaatcc gatttaccta aaagcatttt
     1501 cgtgatttta gataatttct ctctatccgt ccaatcagcg gtgttcaact cttgaatgag
     1561 tttacggcat cgcttcattt cctctaaaat aggtttatca acgatatagg caaggccttt
     1621 attttttcgt tcaatattat ccattaaatg tttactccaa gttggtcatt aactagattt
     1681 taccataatg cacccgcata caaaatatta tctcaatgga ctatttttga atcataagaa
     1741 tgataaattt aatcgacctc tgaacaagat ttcttcaata attgtagtaa gtggcctaaa
     1801 ttaattgtag taaaggatcc acaataattg gagtatgcgt gttttggcct ttaagggacc
     1861 gttatccagg aaatatatat agcaatcgtt tataacctta aatagcgtct ttttcctcaa
     1921 aattacgatt ttcactaatc aaaaaatata ctaaagggca tctgtcgttt tttttggtga
     1981 cactaccgtc ttttagtgag aaaacttgta aacccgtaaa cttatcgtaa ttattatctc
     2041 gaagtaagag ataaggcggt gaagtcatgt attcaaactt agcaagaaga ctagcacttt
     2101 ttaagatatc gcggaaacta ctggcaagag agcgagccag gggcgcagtt tatcgctcaa
     2161 gaattaacca gacgacaatt aagggaagca agactaaaag tagtgatggc atataagaaa
     2221 gccgcaaaac ttggagtcat ggatattgtg tctacgcatg tgagtgtaat ttctatgctt
     2281 ctggaggttc aaaactcaat gaaacagcga ggagctataa atcgttggct ttagaatcaa
     2341 aagatattaa aggttttaaa ttcgtctggt ttaccgatgg gattggctgg gtttccgcgc
     2401 gaaacaattt aaaagagacg tttgatatac ttgatacgct ttataacatt catgatttag
     2461 aaaacaacat tcttgactcc ttcaaggcgt catagtatat aactcgacac atttttgcat
     2521 acaacacatc acgatttatt gtttataact aatttgttcg aaagcgaatt tagagataat
     2581 cagctaagtg atatttaatt gtccaccttt aatttaaaag taccattata tttcatctag
     2641 ctgctaagat aggcaactag attttttaat gtttgaacga ttataaggca ggggttgcac
     2701 tattgaacga ttacagccta atttgtatca attaacgcaa cctttgccat ctgcctagtg
     2761 aagctttgat acaattatca aggctttttt tgtgcttaaa acgctaaaaa taggccattt
     2821 aatactgatt tctatgattt tcggtgatag ttaatagttt tcagggcaaa aaataggggg
     2881 aaatatatac tattgaaaac ctactagctg aacgattagg gggtggggtt gcacgattga
     2941 acgattatag ggagaggggt tgcatttaga gtttaagtcc catcggttgt accatctaac
     3001 tagaataggt ctgatacact aatcaggcct tttttgtgct taaat
//
LOCUS       DAOSEX010000080         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_30583_length_3033_cov_3.000672, whole genome shotgun sequence.
ACCESSION   DAOSEX010000080 DAOSEX010000000
VERSION     DAOSEX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30583_length_3033_cov_3.000672"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..757
                     /locus_tag="PK612_02150"
                     /pseudo
     CDS             1..757
                     /locus_tag="PK612_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208167.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
     assembly_gap    770..779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <780..>3033
                     /gene="parC"
                     /locus_tag="PK612_02155"
     CDS             <780..>3033
                     /gene="parC"
                     /locus_tag="PK612_02155"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOH94946.1"
                     /translation="RKPKKQTEEQVITETIIELPMDEIMGDRFGNYSKYVIQNRAIPD
                     ARDGLKPVQRRIIYAMHDEGNTFNKPMKKCAHTVGAVMGKYHPHGDTSIYDALARMSQ
                     EWSMRYPLIDFQGNNGSIDGDEPAAYRYTESRLNELSNELVLDINKKTVNMNLTFDDT
                     SFEPEVLPSRFPNLLVNGSQGIAVGVATEIPPHNLREVVDAIIHRITYKRSTIEDMRK
                     YILGPDFPTGGVIYKGEGLDSIYETGRGRVEMEALATIESDRTSQRIVITEVPYQSNK
                     SQILRELGAIEQNRNVDGVVEVRDESDRNGIRIVIELKKEANTDVILEYIFSKTRLRA
                     GYNANIVAIADNRPKTLNILDLIDAYISHQVDVITRRSEFDLATNEKRLHIIEGLIKA
                     ISVVNEIIAIIRKSTDKANAKTNIKAAYGFSEEQAEAIVMLQLYKLSNTDITVLMEEK
                     AAIETTIDELRNILSNEKALNNVIIRDLKRIAQKFGDDRRTKIREKGEVVQIDKRDLI
                     ADEQVMLALTRDGYIKRSSLKSYRSSGDFALPGVKSSDLLIAASEVNTKDFLVAFTNL
                     GNYLYIPIYEIGEGRWKDEGKHINYIVQLNANEKIIKAFNVKEFREDLYFAIATRNGM
                     IKRTSLNEFVAVRYSRPLQAMRLLSGDRVADVTLTTGNSELLVITESGAASFYNENEI
                     TVVSVKASGVKSINKKKADKVVALISVDPEVRTRTCIVTSEGHLRLLDHSYFEATARL
                     GRLQHAMRTF"
BASE COUNT         1047 a          503 c          640 g          833 t
ORIGIN      
        1 tgcgcaaaac gaaaaaagtt acgaataatg acatgatttt aagcggtaaa cttacaccag
       61 cgcaaagtaa agattataag aaaaatgaac tctttatcgt taaggtaata gtgctggtgg
      121 tactgctaaa agcggacgtg accggatgta tcaagcagtg ttaccacttc gtggtaaacc
      181 acttaacact gatacaagta ctgttgataa aatgctaaaa aacgaagagt ttgcgacact
      241 aattagcact attggtgctg gctttggcca aaattttgat attgaagatg cccattacgg
      301 caaaatcatt attatgacgg acgctgatta tgacggttca catattcaaa cattattatt
      361 aacctttttc tatcactata tgaaaccact tttaaaagcg gggatgattt atattgccgt
      421 gccgccttta tatcgtgtga gtaaacaagt gaataaaaaa cttatctttg aatacgcgtg
      481 ggatgaccaa agttcagaag cagcgaaaga aaaaattggt cccggttata aaattacgcg
      541 ttataaaggt ttaggggaaa tgaacgctga tcaattatgg gaaacaacac ttaatcccga
      601 aacaagaaca ttaattcgcg ttaatattaa cgatccttta ctcgtagaga tgcgtgttag
      661 taccttgatg gggaatgatg cgaatgctag acgcaaatgg attgaagaaa atgttgactt
      721 taacgcatat gatacattta tcgatgaggt aaaataagat gtcaaaacgn nnnnnnnnna
      781 acgaaaacca aaaaaacaaa ctgaagaaca agtaatcacc gaaacaatta ttgaattacc
      841 aatggatgaa attatgggtg accgttttgg caattattca aaatacgtca ttcaaaatcg
      901 ggcgattcct gatgcacgtg acggcttaaa gcccgtccaa cggcggataa tttatgcaat
      961 gcatgacgaa ggcaatactt ttaataaacc gatgaaaaag tgtgcgcata ccgtgggggc
     1021 ggtgatgggg aaatatcacc cacatggtga cactagtata tatgatgcgc tcgcgcgtat
     1081 gtcgcaagaa tggagcatgc gctatccgct tattgatttc caaggtaata acggtagtat
     1141 tgatggtgat gaaccagcag cctatcgtta cactgaatca cgcttaaacg aactttctaa
     1201 tgaactagtg ttagatatta ataaaaagac tgttaatatg aatttaacgt ttgacgatac
     1261 aagctttgaa ccagaagtat taccatcacg ctttccgaat cttttagtaa acggttcaca
     1321 agggattgct gttggggtgg ctaccgaaat cccgccgcat aacttacgcg aagtagtaga
     1381 cgcaattatt catcgcatta cttataaacg ctcaacaatt gaagatatgc gtaaatatat
     1441 tttaggccca gacttcccaa caggtggcgt catttataaa ggtgaaggct tagatagtat
     1501 ttatgaaaca ggacggggcc gcgtggaaat ggaagcacta gccactattg aaagtgaccg
     1561 cactagtcaa agaattgtca tcactgaagt cccatatcaa tcaaacaaaa gtcaaatttt
     1621 acgggaactc ggagcgattg aacaaaaccg taatgttgac ggtgttgtgg aggtgcggga
     1681 tgagagtgac cgaaacggaa ttcggattgt tattgaacta aaaaaagaag cgaacactga
     1741 tgttatttta gaatacatct ttagtaaaac gcggttacgc gctggctata acgctaatat
     1801 tgtcgcaatc gctgataaca ggccaaagac gcttaatatt ttagatttaa ttgatgcata
     1861 tattagtcac caagttgatg ttattacgcg tcgcagtgaa tttgatctag cgacaaatga
     1921 aaaacgttta catattattg aaggacttat taaagcaatc agtgttgtca atgaaataat
     1981 cgcgattatt cgtaaaagta cagataaagc aaatgcgaaa actaatatta aagcggccta
     2041 tggctttagt gaagaacaag ctgaagctat tgtcatgctc caactttata aattaagtaa
     2101 tactgatatt accgtattaa tggaagaaaa agcggccatt gaaacaacaa ttgatgaatt
     2161 gcgtaatatc ttaagtaatg aaaaagcgct taataacgtt attattcgtg atttaaaacg
     2221 gattgcgcaa aaatttgggg acgaccgtcg tacgaagatt cgtgaaaaag gtgaagtcgt
     2281 gcaaattgat aaacgtgact taatcgccga cgaacaagta atgcttgcgc ttacgcgtga
     2341 cggttatatt aaacgcagta gtttaaaatc atatcggagt agtggtgact ttgccttacc
     2401 tggtgttaaa agtagtgatt tacttattgc cgcaagtgaa gtaaatacaa aagactttct
     2461 cgtagcgttt actaatttag gtaattattt atacataccg atttatgaaa ttggagaagg
     2521 aagatggaaa gacgaaggta aacatattaa ttacatcgtc caacttaacg ctaatgaaaa
     2581 aattattaaa gcgtttaatg ttaaagaatt tagagaagat ttatattttg caatagcaac
     2641 ccgtaacggc atgattaaaa gaacaagtct taatgaattt gttgctgttc gttattcgcg
     2701 cccgctccaa gccatgcggc tattaagtgg tgacagagta gctgatgtca cactgacaac
     2761 gggaaatagt gaacttttag ttattactga aagtggcgct gctagtttct ataatgaaaa
     2821 cgaaattacc gttgtaagcg ttaaagcaag tggtgttaaa tcgattaata aaaagaaagc
     2881 ggataaagtc gtcgcgctca ttagtgttga cccagaagtc agaactagaa catgtattgt
     2941 cactagtgaa gggcacttac gattacttga tcatagctac tttgaagcga ccgctcgcct
     3001 tggaagatta caacatgcaa tgcgcacttt taa
//
LOCUS       DAOSEX010000081         3011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_30906_length_3011_cov_4.284506, whole genome shotgun sequence.
ACCESSION   DAOSEX010000081 DAOSEX010000000
VERSION     DAOSEX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30906_length_3011_cov_4.284506"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="dcm"
                     /locus_tag="PK612_02160"
     CDS             complement(<1..736)
                     /gene="dcm"
                     /locus_tag="PK612_02160"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOH94947.1"
                     /translation="MIQIDYPKSLHKLRRTLNVSQQQLANILNVSFSSVNRWENGHYE
                     PTIIAKVKLDELFDQVKIKPIAKQEKYKSIELFAGAGGLALGLEQAGFDHIGLVEFEK
                     NAVDTLKINRPEWNVLLEDIREVCERDLEQEFNIKKGELDLISGGAPCQSFSYAGNRL
                     GLEDVRGTMFYYYATFLRKLQPKLFLFENVRGLLSHDKGRTYKTILDIFEEQGYITLY
                     EELNAWDYSVPQKRERLITIGIRKDLF"
     gene            complement(940..1167)
                     /gene="secG"
                     /locus_tag="PK612_02165"
     CDS             complement(940..1167)
                     /gene="secG"
                     /locus_tag="PK612_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOH94948.1"
                     /translation="MGTLDIIFIIVSILLIIISLMQGGKSEGASGAITGGGLNIFVKT
                     KERGSEKVISYITYGLAILFFLLAILVKALG"
     gene            complement(1245..>3011)
                     /gene="uvrB"
                     /locus_tag="PK612_02170"
     CDS             complement(1245..>3011)
                     /gene="uvrB"
                     /locus_tag="PK612_02170"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOH94949.1"
                     /translation="QLYAEFKALFPNNRVEYFVSNFDFYQPEAYVPSRDMYIEKEAVQ
                     NAEIEMLRSAAINSLLERNDTIIVASVAAIYGLSDPEEYSKLSFNIVEGEVINRKEFL
                     IKLVDAQYTRNDYELRAGTFRVRGDVIEIMLPISEDYLIQVDTFDDTIEKIREIDVLT
                     GEVRKTFNTYPIFPAYAYASSRERINAIVPKILSDLASRLSYFADEGKLLEAERLEFR
                     TRQDVDSLQEFGICPGIENYARYIDGRKAGEKPYTLMDYFPKDYLLVIDESHVTLPQV
                     RGMFNGDRSRKQNLVDYGFRLPSALDNRPLQFNEFEGMINQVIYTSATPGDYELDKTG
                     GVIVEQLIRPTGLVDPEVFIKPSVGQIDDLMYELTKVIERGERAMVVTLTIKMAEDLT
                     HYLKEHDFKVVYLHNETKTLERSQIIYELRKGKYDVLVGINLLREGLDIPEVSLVAII
                     DADKEGFLRSERSLIQVMGRASRNVNGKVIMYANNMTGSMERAIAETKRRRDLQIAYN
                     KKHGIVPTTIIKPLGEPLHNFGTERELERINKGLMSRKQLEKYIREIRAKMNAAAKAY
                     DFEKAIEYREILFELEGKSKKT"
BASE COUNT          930 a          609 c          421 g         1051 t
ORIGIN      
        1 caaataaatc ttttctaatc ccaatcgtaa ttaatctttc tcttttttgt ggaacactat
       61 aatcccaagc atttaactcc tcataaagtg taatatatcc ttgttcttca aaaatatcga
      121 gaatagtttt atatgttctt cctttatcat gggataataa accacgtaca ttttcaaata
      181 aaaataattt tggttgtaat tttcgcagaa atgtcgcgta ataataaaac atcgttccgc
      241 gtacatcttc taacccaagt cgattaccag cgtagctaaa tgattgacat ggtgcccctc
      301 cggaaattaa atcaagttca ccttttttta tattaaattc ttgctctaaa tcacgttcac
      361 acacctcgcg aatatcttca agaagaacat tccattctgg tctattaatt tttagagtat
      421 caacagcatt tttctcaaat tccactagac caatatgatc aaaacctgct tgttcaagcc
      481 caagcgctaa tccacccgct cctgcgaata attcaattga cttatatttt tcttgttttg
      541 caattggttt aattttaact tgatcaaata attcatcaag ttttactttt gcgataattg
      601 ttggctcata atgaccattt tcccaccggt taaccgatga aaaagaaaca tttaaaatat
      661 ttgctaattg ttgttgtgaa acattaagtg ttcttcttaa tttatgaagg ctttttgggt
      721 aatcaatttg tatcatataa cttatctcgc ttccaaaaat attatagcac tttatttcac
      781 ttttataatt ttaatacttt ccttatttat ttataaataa gtttttgctg tggtttatta
      841 caactactaa taaatacaat aaatttaata aaaacttctc aatattatac ataataaaaa
      901 aacaccctgc cgggtgttaa tttaatagtt ttaataatct taacctaaag ctttaacaag
      961 gattgcaagg agaaagaaca aaatagcaag gccgtaagta atataagaaa ttactttttc
     1021 acttcctctt tcttttgttt tgacgaaaat attaaggccg ccaccagtga tagcgccaga
     1081 tgccccttca cttttaccac cttgcataag cgagataata ataagtaaga ttgatacgat
     1141 aatgaagata atatctaatg ttcccatagt cttcctccag ttctttatta atatacacta
     1201 tttataaatt aattcaagaa aacattattt aaatatacat atatttatgt ttttttactc
     1261 tttccctcta attcaaagag aatttcacga tattctatcg ctttttcaaa gtcataggct
     1321 ttagcggcag cattcatctt agcgcgaatt tcccgaatat atttttctaa ttgtttacgg
     1381 ctcattaagc ctttattaat ccgctcaagt tcacgctctg tcccgaaatt atggagtggt
     1441 tcgccaagtg gtttgataat tgtggtggga acaattccgt gctttttatt ataagcaatt
     1501 tgtaaatctc ttcttctttt tgtttccgca atagcgcgtt ccattgaccc cgtcatattg
     1561 ttcgcataca ttattacttt cccgttaaca tttcgactcg cgcggcccat tacttgaata
     1621 agtgatcgct cactacgtaa aaatccctct ttatccgcgt caataatcgc cacaagtgaa
     1681 acttcaggaa tatctagtcc ttcacgcagt aaattaatcc cgacaagcac atcatatttc
     1741 cctttgcgta attcataaat tatttgactg cgttccagtg tctttgtttc attatggaga
     1801 tagacaactt taaagtcatg ctcttttaag taatgggtta ggtcttccgc cattttaatt
     1861 gtaagcgtga caaccattgc tctttcacca cgttcaataa ctttcgttaa ttcatacatt
     1921 aaatcatcaa tttgtccaac actcggttta ataaatactt ctggatccac taaacctgtt
     1981 ggccggatta gttgttcaac aattacccca cctgttttat ctaattcata atcgccaggt
     2041 gttgcgcttg tgtaaatcac ttgattaatc atcccttcaa attcattaaa ttgtagcggg
     2101 cgattatcta aagcactcgg taatctaaaa ccgtagtcca ctagattttg tttacgactg
     2161 cgatcaccat taaacatccc tcttacctga ggtaaagtaa catgactctc atcaatcaca
     2221 agtaaataat ctttgggaaa ataatccatt aaggtataag gcttttcccc agctttacgc
     2281 ccatcaatat aacgcgcata attttcaatc ccaggacaaa tcccaaattc ttggagtgaa
     2341 tcaacatctt gtcttgttct aaattccagc cgttcggctt caaggagttt accttcatcg
     2401 gcaaagtaac ttaatctact tgctaagtca cttaatattt ttggcacaat ggcatttatc
     2461 cgttcacgac ttgaagcata ggcatatgcg ggaaaaattg gataagtgtt aaatgttttg
     2521 cgcacttcac cagttaaaac atctatttct ctaatctttt caatagtgtc gtcaaatgta
     2581 tcaacttgaa ttaaatagtc ttcactgata gggagcatta tttcaattac gtcaccccgt
     2641 actctaaaag taccagcgcg aagttcgtaa tcattacgtg tgtattgcgc atccacgagt
     2701 ttaattaaaa actctttgcg attaatcact tcaccttcca caatattaaa ggatagttta
     2761 ctatattctt cagggtcact aagcccataa attgccgcga cactagcgac aataattgtg
     2821 tcattacgct cgagtaaact attaatagca gcagagcgaa gcatttcaat ttcggcattt
     2881 tgcactgctt ctttttcaat atacatatcg cgtgatggca cataagcttc tggttgataa
     2941 aaatcaaagt tagacacaaa atattccact ctattattag gaaacaatgc tttaaattca
     3001 gcgtaaagtt g
//
LOCUS       DAOSEX010000082         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_31204_length_2991_cov_4.053815, whole genome shotgun sequence.
ACCESSION   DAOSEX010000082 DAOSEX010000000
VERSION     DAOSEX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31204_length_2991_cov_4.053815"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK612_02175"
     CDS             complement(<1..207)
                     /locus_tag="PK612_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOH94950.1"
                     /translation="MKRKDYINWDEYFMGIAILSSKRSKDPSTQVGACIADDENKVVS
                     LGYNGMPRGIDDDVIPWEKGEDINN"
     gene            complement(274..1905)
                     /locus_tag="PK612_02180"
     CDS             complement(274..1905)
                     /locus_tag="PK612_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002666730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOH94951.1"
                     /translation="MNNKWWHERIFYQIYPRSFKDTNGDGVGDIPGIISKLPYLKSLN
                     VGAIWISPLYASPNFDNGYDISDYKAIHPEYGTMEDMERLLAEAKKLDIKIIMDLVIN
                     HTSFQHDWFLKSKDPKSEYHNYYFWRKGKISKKGKKLPPNNWQSMFTGPAWTYEPSND
                     MWYLHLFTREQPDLNYENPKVLEEVKSILRFWLDKGVAGFRCDVINLISKTSLKDGKK
                     RVYKTGKEHYLNAPGCHLILKELNDEILEPYAAYTVGETMDITIDAAKDYLDGELTQV
                     FAFDQVQVDYFLLPTFKRKYKPHRMVNALKKWQLKIPWNTVFYENHDLPRSVSRFGSK
                     KYYKESVTALLASVLFLRGTPYIYQGQEIGMTNVDFGGIENIEDVSSRNVYNLLRKFK
                     VPRKLAWRFVNNFTRDHGRSPVQWNDKKHAGFTTGKPWLMVNPNYTEINVEAQEIDQD
                     SVLHHYRKMATLRQKTKAFKNGDLEFKKTHRDILAFTRTDDLNEYLVVINLASKARTH
                     KLNLDGEVLYSNYQEFSDKAKKLRPFEVQIIKRLD"
     gene            complement(1992..2120)
                     /locus_tag="PK612_02185"
     CDS             complement(1992..2120)
                     /locus_tag="PK612_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94952.1"
                     /translation="MFEELLFMYDPRTKKEEQREDDDTLEMAQALALYEKHLEDNN"
     gene            complement(2320..>2991)
                     /locus_tag="PK612_02190"
     CDS             complement(2320..>2991)
                     /locus_tag="PK612_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94953.1"
                     /translation="IMASLYLGPVFGAIVGAASDLIGATLFPVGTLIIWPVISSTLYG
                     VVPWLILRLVMYLDRKIKVPLFYVFLAIIFIGLETYIFVKPSIRHPFNSTLDPIMFTT
                     TFRIVFTLVLLLIFSGLIITFNVLVKKYKEGAYEKYTGAPTSLAFTLMLMTFFVDILY
                     SSWWKMFQFKVDFFVSVFFHTLIMFILLPFQVVLLLILSNVYAKSRVAELLALPPKEH
                     IDTDD"
BASE COUNT          966 a          570 c          469 g          986 t
ORIGIN      
        1 attattgata tcttcacctt tttcccacgg aattacatca tcatcaattc cgcgaggcat
       61 cccgttataa cctaaactga cgactttatt ttcatcgtca gcaatacatg caccaacttg
      121 ggtacttggg tctttacttc ttttcgaaga aagaatcgca attcccataa aatattcatc
      181 ccaattaata taatctttac gtttcatgat tagcctccct taacagcgct aattatagca
      241 taaatgctat aggttaaggg ctagttaaga gtcttaatct aagcgtttta taatttgcac
      301 ttcaaatggg cgcaactttt tagccttatc actaaactct tgatagttgg aataaagtac
      361 ttcaccatca agatttaact tatgagtgcg tgctttactt gctaaattaa tgacgactaa
      421 atattcgttt aaatcatctg tgcgcgtaaa cgctaaaatg tcacgatgtg ttttcttaaa
      481 ttctaggtcg ccatttttaa aagcttttgt tttttgccgt aaagtcgcca tttttcgata
      541 atgatgtaat actgagtctt gatctatttc ctgagcttca acattaattt ctgtataatt
      601 tggattcacc attaaccacg gtttaccagt agtaaaacca gcgtgctttt tatcattcca
      661 ctgtaccgga ctgcggccat gatcacgcgt aaagttattc acaaagcgcc acgcaagttt
      721 acgcggtact ttaaatttac gcagtaagtt ataaacatta cgcgaactta catcttcaat
      781 attttcaatg ccgccaaaat caacatttgt cattccaatt tcttgtcctt gataaatata
      841 cggtgttccg cgtaagaata agactgacgc taaaagtgcc gtaacacttt ccttataata
      901 ctttttacta ccaaagcgac tcacactacg tggcaagtca tgattttcat aaaagactgt
      961 attccaaggt attttaagtt gccatttctt tagggcgtta accatccgat gcggtttata
     1021 tttccgttta aatgttggta ataaaaagta atcgacttgc acttgatcaa aggcaaacac
     1081 ttgcgttaat tcaccatcta aataatcttt agcagcgtca atagtaatat ccattgtttc
     1141 gccaaccgtg taagccgcgt aaggttctag tatttcatca ttaagttctt ttaagattaa
     1201 gtgacaccca ggtgcattta agtaatgctc ttttcctgtt ttatacacgc gcttcttccc
     1261 gtctttaagt gaagttttac taattaaatt aataacatca caccggaaac ctgcgacacc
     1321 tttatcaagc caaaagcgta aaatactttt aacttcttct agtacttttg ggttttcata
     1381 atttaaatca ggttgttctc gtgtaaaaag atgtaagtac cacatgtcat tacttggttc
     1441 ataagtccag gctggtccag taaacattga ttgccaatta ttcggcggta attttttacc
     1501 ttttttacta attttacctt tacgccaaaa gtaatagtta tgatattcac ttttgggatc
     1561 cttgctcttt aaaaaccaat catgctggaa ggaagtatga ttaataacta agtccataat
     1621 aattttaatg tcaagtttct tagcttccgc gagtaatcgc tccatgtctt ccatcgtgcc
     1681 atattctgga tggattgctt tataatcact aatgtcgtac ccattatcaa aatttgggga
     1741 agcgtaaagc ggagaaatcc atattgcccc aacatttagt gattttaaat atggtaattt
     1801 agaaataatc ccaggaatat caccgacacc atcaccattt gtgtctttaa aactccgcgg
     1861 ataaatttga tagaaaatgc gttcatgcca ccatttatta ttcataattt tgtacctctc
     1921 ttttagtgta tcaaaaaagc aataaaaaaa cgccctaagg cgtaatttat taaaagtcac
     1981 aataactttt attagttatt atcttctaaa tgcttttcat aaagagcaag cgcttgtgcc
     2041 atttccagcg tgtcatcatc ttcgcgttgt tcttcttttt ttgttcgcgg atcatacata
     2101 aataaaagtt cttcaaacat aagcatttac tcctttttta actacgacta ttgtaagata
     2161 atagccccta aaattcaaag gtatttaaga cactaataat taatactaca agtattaatt
     2221 ttgacatatt gtgtctaatg cgtttaggag ttttttagta gtttttaaaa aaggcgttaa
     2281 ttcacgccta acttaaggtt taaaacatta taaaaaggct taatcgtctg tatctatatg
     2341 ttcttttggg ggtagcgcta aaagttctgc tactctgctt ttggcgtata cattacttaa
     2401 aattaataaa agcacaactt ggaatggtaa taaaataaac atgattaaag tgtggaaaaa
     2461 gacactgacg aaaaaatcaa ctttaaattg gaacatcttc caccatgacg aataaagaat
     2521 atcgacaaag aaagtcatca gcatcagtgt aaacgctagt gaagttggtg caccagtata
     2581 tttttcataa gcaccttctt tatatttttt aacaagcaca ttaaaagtaa taatgagtcc
     2641 gctaaagata aggagtaaaa caagcgtaaa aacaattcta aaagttgtcg taaacataat
     2701 cgggtcaagc gtgctattaa acgggtgtct aattgaaggc ttaacaaaga tatatgtttc
     2761 taagccaatg aaaataatag ctaaaaatac atagaaaagc ggtactttga tttttctatc
     2821 taaatacatc actaggcgta aaataagcca cggcacaaca ccataaagtg ttgaactaat
     2881 cactggccaa ataataaggg ttccgaccgg gaaaagtgtc gccccaatta aatcacttgc
     2941 cgcgcccaca atcgcaccaa atacggggcc aagataaagt gaagccataa t
//
LOCUS       DAOSEX010000083         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_31848_length_2950_cov_3.396200, whole genome shotgun sequence.
ACCESSION   DAOSEX010000083 DAOSEX010000000
VERSION     DAOSEX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31848_length_2950_cov_3.396200"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /gene="pyrH"
                     /locus_tag="PK612_02195"
     CDS             <1..460
                     /gene="pyrH"
                     /locus_tag="PK612_02195"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOH94954.1"
                     /translation="KDYFTQNDLPAVVLNAFAVEGLAEKTSPELAKKHLAKGEVVIFG
                     GGTGKPFYTTDTGAALRAIDMNADLIIFVKDGVDAVYIKNPHQYKDAQKITQVTYQEL
                     IDLKVEAMDLSALEVLNKTNITSFVIGMDEVLNFEPLEEHMEIGTIITKR"
     gene            466..1011
                     /gene="frr"
                     /locus_tag="PK612_02200"
     CDS             466..1011
                     /gene="frr"
                     /locus_tag="PK612_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOH94955.1"
                     /translation="MDEYALLAEERMEKSILNLQSEFSTLRTGRASAALLDRIECDYY
                     GEKMPINQISSISVPEPRQLLIKPYDKGDMRSILAAINASTLGINPINDGDTIRLILP
                     PLTEERRRDLVKQAKKFTEEAKIAIRNIRRDANTSIKNDKDLSEDMSKRAEEAVQKVT
                     DEYVKKADDLLKEKEEEILTV"
     gene            1374..2093
                     /gene="uppS"
                     /locus_tag="PK612_02205"
     CDS             1374..2093
                     /gene="uppS"
                     /locus_tag="PK612_02205"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOH94956.1"
                     /translation="MSKTKITNYENYGIKHIAFILDGNGRWAKARKRPRTYGHEKGFS
                     SLFDVGIAVSKRNIPFMSVYAFSTENWSRPKKEVDFLFRTLEKRLHSVLKKVQKHNIK
                     IVTSGDLKRLPESTQQAINYAKEESKNNTGTTLNICINYGGKDEIVRAIKRLAADESV
                     NIQALTPDTFENYLDTSRMPPVDLLVRTSGEMRLSNYMLWQLAYAEIIFTPTYWPDYD
                     EECLVRDLEEYAKRTRRFGGL"
     gene            2096..>2950
                     /locus_tag="PK612_02210"
     CDS             2096..>2950
                     /locus_tag="PK612_02210"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOH94957.1"
                     /translation="METNEIKPKTKRTSTIKISLNEISEATKKSMKTRTITSIILAVI
                     AVPALLLGGWYFLALVFFVSIIAVLEIMRGTKISKRYWCVYILAIVSTVALIFWIFIK
                     NNIMANIKAGYSWWDISKWVVENGFESIQVSSFLLATILIGLFILIVFDPHLGFDIAA
                     YLFLLVLFAGFGFQAFLFLRLYPQYIADRPDITFNTNIITSAFLIIYVILGTIMNDIG
                     AYFIGVLFGKNKMVPRISPNKTWEGFAGGVFFSIVVSFLFALIVSWIGYPVLPFLSHK
                     EWYYLLLVS"
BASE COUNT          987 a          450 c          571 g          942 t
ORIGIN      
        1 aaaagattat tttacgcaaa atgatttacc tgctgttgtc ttaaacgctt ttgcggttga
       61 aggacttgcg gaaaaaacta gtccagaatt agcaaaaaaa catttagcga agggagaagt
      121 tgtcattttt ggtggtggta caggtaaacc gttctacacg actgatacag gtgcagcact
      181 ccgcgctatt gatatgaatg ctgatctaat tattttcgta aaagacggtg ttgacgctgt
      241 ttacattaaa aatccgcatc aatataaaga tgcacaaaaa ataacccaag tcacttacca
      301 agaactaatt gatcttaaag ttgaagcgat ggatttaagc gccctagaag tactaaataa
      361 aacgaacatc acaagttttg tcattggtat ggatgaagtt ttaaacttcg aaccacttga
      421 agaacacatg gaaattggta caattataac gaagaggtaa aaaatatgga tgaatatgca
      481 ttactcgctg aagagcgaat ggaaaaatca attttgaatt tacaaagtga attttcaaca
      541 ctccgcaccg gacgggcaag tgcggcgtta cttgaccgga ttgaatgtga ttattacggt
      601 gaaaaaatgc caattaatca aattagttca attagtgtcc ctgaaccgcg tcaactttta
      661 attaagcctt atgataaagg tgatatgcgt tcaattttag cggcaattaa cgcctcaaca
      721 ctggggatta acccgattaa tgatggcgat acaattcgct taatcttacc cccacttacc
      781 gaagagcggc gccgtgattt agtgaaacaa gctaaaaagt ttacggaaga agcaaaaatt
      841 gccattcgta atattcggcg tgacgctaat acgagtatta aaaatgataa agatttaagt
      901 gaagacatga gcaaaagagc agaagaagct gtgcaaaaag ttactgacga atatgtcaaa
      961 aaagctgatg atttacttaa agaaaaagaa gaagaaatct taaccgttta atgtaaatta
     1021 acaactatga attaagcctt ttacaaggct tttttctttt gtaacacact agtgtgttat
     1081 cctaaaatta atataagagc caaaagtaat aagtaaatcg cggaaaacat tattattcta
     1141 gcgaagaaga cctgcggctt atgtgggtta agaagcgaaa agtctatact gatacaatca
     1201 taaaatttaa tgaaaaagta gcgatctctt tcccatcttt tgataaagat tatccgttgt
     1261 atccttatta aagctattag tgaaataaat tatttttaca aacgcttttc ttttttaaca
     1321 ttttaaaaat gttatttaat gaaaatagtt attcttattg tataatttaa catatgagta
     1381 agacgaagat tacaaactat gaaaattatg gcattaaaca tattgctttt attttagatg
     1441 gtaatggtag atgggcaaag gcgcgtaaaa gaccaagaac ttatggtcat gaaaaaggtt
     1501 tttccagtct ttttgatgtt ggtattgctg ttagtaaacg taatatcccc tttatgagtg
     1561 tatacgcttt ttctacagaa aactggtcac gaccaaaaaa agaagtcgat tttttattta
     1621 gaacactcga aaaaagatta catagtgtgt taaagaaagt acaaaaacat aatattaaaa
     1681 ttgtgacttc tggtgactta aaaagattac ctgaatcaac acaacaagcg attaattacg
     1741 caaaagaaga atcaaaaaat aatacgggca ccacattaaa tatttgtata aattatggtg
     1801 gtaaagatga aatcgttcgt gcgattaaaa gactagcagc agatgaaagt gttaatattc
     1861 aagcattaac acctgatact tttgaaaatt atttagatac aagcagaatg ccgccggttg
     1921 atcttttagt gcggacgagt ggcgagatgc gccttagtaa ttatatgctg tggcaactag
     1981 cctacgctga aattatattt accccaactt attggccgga ttatgacgaa gagtgtctcg
     2041 tccgtgattt agaagaatac gcaaaaagaa cgcgcaggtt tggaggatta tagtgatgga
     2101 aactaatgaa attaaaccaa aaaccaaacg gacttcaacg attaaaatat cactaaatga
     2161 aattagtgaa gcaacaaaga agtcaatgaa gacccgcaca attacttcga ttattttagc
     2221 ggtaatcgct gttcctgcct tgcttttagg tgggtggtac tttttagcgc ttgtgttctt
     2281 tgtgtcaatt attgctgtgc ttgaaattat gcgtggcacg aaaatatcaa aacgctactg
     2341 gtgtgtgtat atcttagcga ttgtttcaac tgttgccctt atcttttgga tttttattaa
     2401 aaataatata atggctaata ttaaggccgg atactcgtgg tgggatattt caaagtgggt
     2461 ggtggaaaac ggttttgaat caattcaagt atcaagtttc ttacttgcga ccattttaat
     2521 tggtctattt atcttaattg tgtttgatcc gcatcttggt tttgatatcg cggcgtattt
     2581 attcttgtta gtgctctttg ccgggtttgg gttccaagct ttcttattct taaggcttta
     2641 tccgcaatat attgctgatc gtcctgacat tacttttaac acgaatatta ttacgagcgc
     2701 attccttatc atttatgtaa ttttaggaac aattatgaat gatattggtg cttattttat
     2761 cggtgtgtta tttggtaaaa ataagatggt accacggatt tccccaaata aaacatggga
     2821 aggctttgct ggtggtgttt tcttttcgat tgttgttagt tttttattcg ccttaattgt
     2881 gtcgtggatt ggttatccgg ttttaccatt tttgagtcat aaagagtggt attatttatt
     2941 actcgtctca
//
LOCUS       DAOSEX010000084         2941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_31988_length_2941_cov_3.765766, whole genome shotgun sequence.
ACCESSION   DAOSEX010000084 DAOSEX010000000
VERSION     DAOSEX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31988_length_2941_cov_3.765766"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2292)
                     /locus_tag="PK612_02215"
     CDS             complement(<1..2292)
                     /locus_tag="PK612_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94958.1"
                     /translation="MFIYFDRNGVLQEVINDKAARLGDLNANEIYVYVDEVELDDIWL
                     TLKRPDGTLSVEKSFIANKVVREIPYDPKRDMKFFKDFKEYEFYKFTITDEITSAGVY
                     QATIRMTYEDTLSALGVLVFKAETGVIKSDQNITQAQYDYLIWYVGSWQGQIGDLEEA
                     MGIAEAGILGLQNRMNVAEQDINKIEDGTTVVKKALKDDLGNTIKSTYANKLDLVYSS
                     SNNRIAITLKSYDGTPIDADLIDLPKATTSADGLLAKEDKVKINNIATDIASMGATKQ
                     SLSEKNQINGYAGLDGNAKIPSSLLPDSVLGQLEYKGTFNPNNGYPSSPEKGWFYIAI
                     ADALISGVDYQVGDWAVFNGSTWDKIDNTDAVMSVNGKLGVVVLNGTDIPVGNGDTWT
                     IEQRIVNIYAELNNRYTKAQTYSNTEIEQRITDLLGMVNLQDTAVEFSAGVYDLSYNG
                     LAVSTFDYSLVSPYNWVILRVLVGTQAEYTIFKPSEATNGVGINLPLTQKDVYAEIMR
                     NGSNFEFSLKKKNDNTLDTTTATMKAWGIKLDGIDASDISYNNETVESALDSLLVPEY
                     ITATHEALQSEITATNNIEIKDDSGELVKITGNTPTNLVSNSELLIDSNSDGVPDGFS
                     YNNATDISLTNGIAKFTATATAGRLEYRLNDTDTKTIYVYARIKSISNTTYLRTSDSN
                     SHYAYHSGNSEYELLSDIYIASSTYNFIAIGSSASSGWTPISVDYIGAYNVSDMIRQG
                     VKNDDGIPFADLTNEEIKDQLDIW"
     gene            complement(2296..>2941)
                     /locus_tag="PK612_02220"
     CDS             complement(2296..>2941)
                     /locus_tag="PK612_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94959.1"
                     /translation="TAGVDSEEFKAADEINNKIIKSVYNEMVEFRKYVKTLNYNEMVR
                     LQEDFLYKYGVDDVSELNKKQLRKFKRLKPIRHDISGFNLPLYYELTKNGEIKYESSF
                     NKNKGMLRKRAGKVLTGILFGALSVNIAFNIDGLGEAMMSVIVISTNLIITYLLSFMP
                     PMFRLKFVIPKGVILKHTLYSGYIEYKKGTHILKEIPMKKEVLEVVEQEKTQEE"
BASE COUNT          857 a          622 c          440 g         1022 t
ORIGIN      
        1 ccaaatatct aattggtctt tgatttcttc gttggttaaa tcggcaaagg ggataccatc
       61 atcattttta acaccttgtc taatcatatc gctgacattg taagcaccaa tgtaatcgac
      121 tgaaataggt gtccaaccag aactcgctga tgaaccaata gcgataaaat tataagtgga
      181 agaagcgata tatatatctg ataaaagttc atattcacta tttccgctat gataggcata
      241 atgactatta gaatcagaag tccttaaata agttgtattt gatatacttt taatgcgtgc
      301 gtatacatat attgttttag tgtcggtatc atttaatcta tattctaaac gaccagccgt
      361 agcagtagcc gtaaacttcg caataccatt cgttaatgat atatcggtag cattattata
      421 tgaaaaccca tcgggaacac catcactatt actatcaatt aacaattcgc tattgctaac
      481 taaattagta ggtgtattac ccgtaatttt aactaactcg ccactatcat ctttaatttc
      541 aatattatta gtggcagtga tttcactttg aagtgcttcg tgtgtagcag ttatatattc
      601 aggaactaat agactatcta atgcactctc aactgtttcg ttgttgtaag atatatcact
      661 tgcatcaatt ccatctaatt taatgcccca cgctttcata gtcgcagtcg ttgtatctaa
      721 tgtattatcg ttctttttct ttaacgagaa ttcaaaattt gaaccattac gcatgatttc
      781 agcgtagaca tctttttgcg ttaatggcaa gttaatgcca acgccattcg ttgcttcact
      841 cggcttaaaa atcgtatatt cggcttgtgt ccccactaac actcgtaaaa taacccagtt
      901 atatgggcta actaaactat aatcaaatgt actaaccgct aaaccgttat aagataaatc
      961 ataaacccca gcactaaact cgactgccgt atcttgtaag ttcaccattc ctaacaaatc
     1021 ggtaatgcgt tgttcgattt cagtattaga atatgtttgt gctttcgtat agcgattatt
     1081 tagttcagcg taaatattaa cgatgcgttg ttcgatcgtc caagtgtcgc cattaccaac
     1141 cggaatatca gtgccattaa gcacgacaac ccctaattta ccattaacac tcataaccgc
     1201 atcggtgtta tcgattttat cccaagttga tccgttgaaa accgcccagt cgcctacttg
     1261 ataatccact ccactaatta aagcatcagc aatcgcaata taaaaccaac ctttttcagg
     1321 acttgatgga taaccattat ttggattgaa tgttccttta tattctaatt gccctaaaac
     1381 gctatctggt aaaagagaac tcggtatttt agcgttgcca tcaagtccgg catagccgtt
     1441 aatttgattt ttttcactta aactttgttt agtcgccccc attgaagcga tatcagttgc
     1501 gatattatta attttaactt tatcttcttt ggctaaaaga ccgtccgcac tcgtggtcgc
     1561 ttttggtagg tcgattaaat cggcatcgat tggagtgcca tcataacttt ttaatgtaat
     1621 cgcaattcga ttattactac ttgaatagac taaatcgagt ttattcgcat aggtgctttt
     1681 tatcgtatta cctaaatcat cctttaacgc ttttttgaca acggttgtgc catcctcaat
     1741 tttattaatg tcttgttcgg caacattcat gcgattttgt aaacctaaaa taccagcttc
     1801 agcaatcccc attgcttcct ctaaatcgcc gatttgccct tgccaactgc ctacatacca
     1861 aatgagataa tcatattggg cttgggtaat gttttggtcg cttttaatta caccggtttc
     1921 cgctttgaat actaaaaccc ccaacgcact taaagtgtcc tcgtaggtca ttctaatcgt
     1981 tgcttggtaa actcccgcac tcgtgatttc atccgtaata gtgaatttat aaaactcgta
     2041 ttctttgaaa tctttaaaga acttcatatc tcgctttgga tcatacggaa tttcccgcac
     2101 taccttattt gcgataaatg atttctcgac cgataaagtg ccatctggtc ttttaagcgt
     2161 gagccaaata tcatctaact ccacctcatc tacatagaca taaatctcgt ttgcattcaa
     2221 atcgccaagt cttgccgcct tatcgttaat gacttcttgt aagactccgt ttctatcaaa
     2281 ataaataaac atagtttact cctcctgtgt cttttcttgt tcgacaactt ctaatacttc
     2341 ttttttcatc gggatttctt ttagaatatg cgttcccttt ttatattcga tatatccgct
     2401 atataaagta tgctttaaga taacaccttt tggtataaca aattttaatc taaacatagg
     2461 cggcataaac gataataaat aggtaataat aaggttagtc gaaattacga taacactcat
     2521 catcgcctcg ccgagaccat caatattgaa tgcgatgttc acgcttaacg caccgaatag
     2581 tatgccagtt aataccttac ctgctctttt tctcagcatg cccttattct tattaaatga
     2641 actttcatat tttatttcgc catttttagt aagctcgtaa tataaaggta agttgaagcc
     2701 gctaatatcg tgtctaatcg gttttaatct tttgaacttt cgcagctgtt tcttatttaa
     2761 ttcgctaaca tcatcaacac catatttata taagaagtcc tcttgcagtc taaccatctc
     2821 gttataattt aaagtcttga catattttct aaattcgacc atttcattat aaacgctttt
     2881 gatgatctta ttattaattt cgtcagctgc cttaaactcc tcactatcaa caccggctgt
     2941 t
//
LOCUS       DAOSEX010000085         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_32007_length_2940_cov_3.978856, whole genome shotgun sequence.
ACCESSION   DAOSEX010000085 DAOSEX010000000
VERSION     DAOSEX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32007_length_2940_cov_3.978856"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..2539
                     /locus_tag="PK612_02225"
     CDS             53..2539
                     /locus_tag="PK612_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOH94960.1"
                     /translation="MEEFVETKKKRPGTATTIIKKSEIKVDEVIRYTPKHDEGLSTQQ
                     VNARKVAKMTNEKPNKGVKSIFSILMRNFFSFFNVLLYAIGAALIYIKEWQSLLFLVI
                     LFSNILIGLIQDFRARRTLKKLLILNEQKVTVVRDGIELLIPSEDVVLDDIVKLKLGD
                     QIAADGVVVHGSVGVNESLLTGEPNIIHKAKDDRVLSGSFVTTGTCYYQVDTIGKLGY
                     AQKLQLRAKVFKRPTSELLNSINSLFKVIGFIVIAMALLLVFNAFYQGSTFEDSVRSI
                     AGSLVAMIPSGMYLLTSMTLAVGVIRLGTKNTLVNEMYSIEMLARVNVLCLDKTGTIT
                     DGTMKVNEVVPLKGAKDIDLNIIVASILRATKDENATAIALKNHFKTRKYMEYTKVLP
                     FDSELKCSGAIMEGKTYIIGASSMVLGPKAQKQVEEVERIYLERGLRVLVLASSRKPF
                     IAKKLPIDMTPLALIVIQDNIRAEASEVIKLFQDAGVEVKIISGDDPVSVSEIARQVG
                     INNAENLLDFSKIDDPSHHSLEDFTIYGRVRPEQKEGIVTKLQSDGKVVAMVGDGVND
                     VLALKAAQCSIAMGAGSPAARGVSHLVLLDNNFDVLPSIVDEGRRAINNLQKTWSLFL
                     VKTIFAIIFSFMFVTAGLLGPKGSGIRYPFEPKHLYLWELLSLGIPAFFLSMQPNKER
                     VRGTFMDNVISRSVPAGLLMVAGAFLLYISKNFGEMFGAEISDTSMEMITMSILTITF
                     LSFVILYRNSLPFNRYRLTLFLVLSLLAIGVIILDNTVGLTFTEKYFLEINISSLEPI
                     HYAILIPINLILTIMYILVDNKIVKKLRRE"
     gene            2543..>2940
                     /locus_tag="PK612_02230"
     CDS             2543..>2940
                     /locus_tag="PK612_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOH94961.1"
                     /translation="MKIRISQEVAYALANNLPVVALESTIISHGMPYPQNVEIALKVE
                     EIVRANGALPATIAIIGGDVVVGLDEDEIEELGQRKDVIKVSRRDLAYVMATKKWGAT
                     TVAATMVIAAKVGIKVFVTGGVGGVHRGAT"
BASE COUNT          961 a          485 c          615 g          879 t
ORIGIN      
        1 aaagatccag tcgtagcgct gcgcactgag taatgaatag aaaggactca ttatggagga
       61 atttgtagaa acaaagaaaa aaagaccggg tacggcaaca acaatcatta aaaaaagcga
      121 aattaaagtt gatgaagtaa ttcgttatac accaaaacac gatgaaggct taagtaccca
      181 acaagttaac gcccgcaaag tagcgaaaat gactaacgaa aaaccaaata aaggcgttaa
      241 gtcaatcttt tcaatattaa tgcgtaattt cttttcgttc tttaatgttt tgctctacgc
      301 aataggagca gcgcttattt atattaaaga gtggcaaagt ttgttatttc tcgtcattct
      361 tttttccaac attttaatcg gacttatcca agactttagg gcgcgacgca cactaaagaa
      421 actcttaata ttaaatgaac aaaaagtaac ggtcgtgcgc gatgggattg agttattaat
      481 tccaagtgag gatgttgtac ttgatgatat cgttaaatta aaattaggtg atcaaatcgc
      541 agcagatggt gtggtagtgc acggtagtgt tggtgttaat gaatcactat taactggtga
      601 accaaacatt attcataaag caaaagatga ccgcgttttg tcggggtctt ttgtcacaac
      661 aggcacatgt tactatcaag ttgatacaat tggaaaatta ggttatgcac aaaaattaca
      721 attacgggcg aaagtattta agcgtccaac atctgaactt ttaaattcaa ttaattcact
      781 atttaaagta attgggttta ttgtgattgc tatggcatta ctattagtgt ttaatgcctt
      841 ttatcaaggc tcaacatttg aagatagtgt gcgctctatt gccggttcac ttgtggcaat
      901 gattccttct gggatgtatt tattaacaag tatgacgctt gccgtaggag ttattagact
      961 cgggacaaaa aacacacttg tgaacgaaat gtattcgatt gaaatgttag caagagttaa
     1021 tgtactctgt ttagataaaa caggcacgat tactgacggc acaatgaaag taaatgaagt
     1081 tgtgccactt aaaggcgcaa aagacattga tttaaacatt attgttgcct caattttaag
     1141 agccactaaa gatgaaaacg ccactgctat agcactcaaa aaccatttta aaacgagaaa
     1201 atatatggaa tacaccaaag tgttaccgtt tgatagcgaa ctaaaatgta gcggtgcaat
     1261 tatggaaggt aaaacttata tcattggtgc ttcaagtatg gtgcttggtc caaaagcaca
     1321 aaaacaagtt gaagaagttg aacgcattta cttagagcgc ggcttacgtg tattagtact
     1381 tgcttcgtct cgcaagccgt ttatcgctaa aaaactacca attgatatga cccctttagc
     1441 acttattgtt attcaagata atattcgcgc tgaagctagt gaagtcatta aactattcca
     1501 agatgctggt gttgaagtaa aaattatttc cggagacgat cctgttagcg tgagtgaaat
     1561 agcgcgtcaa gtcgggatta ataatgccga aaatttatta gacttttcta aaattgatga
     1621 tccaagtcac cactcacttg aagactttac aatttacggc agagtgcgtc ctgaacaaaa
     1681 agaaggtatt gtaactaaat tacaaagtga cggtaaagtt gtagcgatgg ttggggacgg
     1741 cgtaaatgat gtcttagcat taaaagctgc gcagtgttca attgcaatgg gcgctggtag
     1801 tccagcagca cgcggagtca gtcacttagt gctccttgat aataactttg atgttttgcc
     1861 ttcaattgtt gatgaaggac gaagagcaat taataactta caaaagacat ggtcattatt
     1921 ccttgttaaa acaatcttcg cgattatctt ctcgtttatg tttgttactg cgggcctttt
     1981 aggaccaaaa ggatcaggca ttagatatcc ctttgaacca aaacacttgt atttgtggga
     2041 attattatca ttaggaattc ccgccttctt cttatcgatg caaccgaata aagaaagagt
     2101 gcgcgggaca tttatggata atgtcattag tcgcagcgtg ccagcaggat tgttgatggt
     2161 cgctggagca tttttactct atataagtaa aaactttggc gaaatgtttg gtgcagaaat
     2221 aagtgataca tcaatggaaa tgattacaat gtcgattctt acaattacat tcttgtcatt
     2281 tgttatttta tatcgaaact cattaccatt taatcgttat cgactgacac ttttcttagt
     2341 actaagcctt ttagcaattg gtgttattat tctcgataac actgtcgggt taacatttac
     2401 ggaaaaatat ttcttagaaa ttaacatctc aagtttagaa ccaattcact atgcaatatt
     2461 aattccaata aatttaatac ttacaataat gtacatatta gtggataata aaattgtaaa
     2521 gaaattaagg agagaataat ttatgaaaat acggattagc caagaagtag cctacgcttt
     2581 agcgaacaac ttaccagttg tagcgctaga atcaacgatt attagtcacg ggatgcctta
     2641 cccgcaaaat gtggaaatag cgcttaaggt tgaagaaatt gtgcgcgcta atggggcgct
     2701 accagcaaca atagcgatta ttggtggtga cgttgtcgtt ggtttggatg aagatgaaat
     2761 tgaagagtta ggccaaagaa aagatgtcat taaagtttca cggcgcgacc tagcctatgt
     2821 tatggccaca aagaagtggg gtgcgacgac cgttgctgcg accatggtta ttgctgctaa
     2881 agtaggcatt aaagtatttg taactggtgg cgttggcgga gtgcatcgcg gtgcgactga
//
LOCUS       DAOSEX010000086         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_32442_length_2914_cov_2.785589, whole genome shotgun sequence.
ACCESSION   DAOSEX010000086 DAOSEX010000000
VERSION     DAOSEX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32442_length_2914_cov_2.785589"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="PK612_02235"
     CDS             <1..1553
                     /locus_tag="PK612_02235"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOH94962.1"
                     /translation="LLQLAISMDYSIFLLHAYQKERAEGLDKKDAMVMAMRKSFVSIN
                     SSMLTTAAGFLALMFMRYTIGIDLAAVMIKGILLSIISTFTFMPGLILLTDKIVEKTK
                     HKRFFPLLGGLTKWIVKLRYVVPAILLLVIVPSYLAQTNNSFVYGEAAMSVSEGTPAA
                     IEKNRIEAKFGKENLMVILVPRDEADAYIAQEKAMIKQLYREFERANQDFVVQITSLS
                     NLSDIETYIPNYDDLDQTIKNFLDDIFADIMDNLITEEYKAQFLSEHYSRIIITVNTE
                     SESPRAEIAVDIVETVVARQPFARDAHIIGVSPSVKEIRNVVETDYVIVDLISIGFVF
                     IILLVSFRSISLPFILVLVIQISIWINMAIPYLTSQPMIFIGYMIVSSVQLGATIDYA
                     ILFTGFYTEGRKTMIRRDAIKYALDSGGHSILTSCLILTSAGYSLKFLSSIEGVSSLG
                     ELIGRGAALSGILVLVLLPQLLYIFDKVVEKTTWKMRFYKGGTKVGRVIIEDEGYSNS
                     DQDPTLLS"
     gene            1644..2660
                     /locus_tag="PK612_02240"
     CDS             1644..2660
                     /locus_tag="PK612_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOH94963.1"
                     /translation="MIDDPQTWVLLILYFVAGMYFSSSETAFMTANRFKIRAKAEEGS
                     VSAKLAAWVINRLENSIIAVVVFNNLAHLLFTALLTVVLVNQYGNDTGELLATLISVP
                     LMFLFAETIPKVLGRRYSEQYVLISAYLLVPFIIISYPVALFFRGIVWVVKKLFKATE
                     DNIFTADDFEQVIETIEEHGIINEDASDIIISSLEFSETVVKDVITPKNAIVAYDISK
                     FNKDSFKDFVLSTHFSRIPLYDGSLDKIVGVILVREYIKHYQKKPNINPRTIMARPYY
                     VNYKITLTKMVEGFKKNNTHIAFVVDDKKKLLGMVTMEDVLEELVGQIAETSGTVKGA
                     SVNG"
     gene            2653..>2914
                     /locus_tag="PK612_02245"
     CDS             2653..>2914
                     /locus_tag="PK612_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF21 domain-containing protein"
                     /protein_id="HOH94964.1"
                     /translation="MGNVIRFTIVILLFALSFFFSSSESAFSTINRLRVERDVEDGKK
                     RSKTLLRIITNYENTVQTILLGNNFVNIAVTALVTGMALAYDN"
BASE COUNT          863 a          474 c          606 g          971 t
ORIGIN      
        1 cactcttgca attagcaatc tcgatggatt attccatatt cctcttgcat gcttatcaaa
       61 aagaaagggc agagggactt gataaaaaag atgcaatggt gatggcaatg cgcaagtcat
      121 ttgtgtcaat taactcgagc atgttaacaa ctgcagccgg atttttagca ctgatgttta
      181 tgcgttacac aatcgggatt gacctcgcgg cggtgatgat taaagggatt ttgcttagta
      241 ttatctcaac ctttaccttt atgcctggtt taatcttgct cactgataaa attgtggaaa
      301 aaacaaaaca taaacgcttt ttcccacttc ttggtggact cactaaatgg attgtaaaat
      361 tacgctatgt tgtgcctgcg atacttttac tcgtaattgt gccttcttac ttagcacaaa
      421 ctaataatag ttttgtgtat ggtgaagcag cgatgagtgt gagcgaaggc acaccagcgg
      481 caattgagaa aaatcggatt gaagcgaagt ttggtaaaga aaacttaatg gttattcttg
      541 taccacgcga tgaagcagat gcttatattg cccaagaaaa agcgatgatt aaacagcttt
      601 atcgcgagtt tgagcgcgct aatcaagatt ttgttgtaca aattacgagt ctttcaaact
      661 taagtgatat tgagacttat attcctaact acgatgattt agatcaaacg attaaaaatt
      721 tccttgatga tatttttgcc gatattatgg ataatcttat taccgaagaa tataaggcac
      781 agtttttaag tgaacattat tcacgcatta ttattactgt taacactgaa agtgagtcac
      841 cgcgggcgga aattgctgtt gatattgttg aaactgttgt cgcccgtcaa ccttttgccc
      901 gtgatgcaca tattattggt gtctcgccat cagttaaaga aattcgtaat gttgtcgaga
      961 ctgactatgt gattgtagac ttaatttcaa ttggctttgt atttatcata ttacttgtta
     1021 gttttcgcag tatctcattg ccatttattt tagtgcttgt tattcaaatt tcgatttgga
     1081 ttaatatggc aattccgtat ttaacgagtc aaccgatgat ttttattggt tatatgattg
     1141 taagttcggt acaactcggg gcgacgattg attacgctat actttttact ggattttaca
     1201 ctgagggacg aaaaacgatg attagacgtg atgcaattaa gtatgccctt gatagcgggg
     1261 gtcatagtat tttaacatcg tgcttaattt tgacctctgc tggttatagt ctaaaattcc
     1321 tctcaagtat tgaaggcgtg tcctcgcttg gtgaactaat aggccgtggt gcagcactta
     1381 gtggtatcct tgtgcttgtg ttattaccac agttgctata tatcttcgat aaagttgtgg
     1441 aaaaaacaac atggaagatg cgcttttata agggcgggac gaaagttgga cgggttatta
     1501 ttgaagatga aggttatagt aatagtgatc aagacccaac cttgcttagt taactttagc
     1561 gttgcacaaa tttcggcgct gtgataacat actgtcgttg catatgaata tgcacattta
     1621 taaagaagga gtgtggtttc taaatgattg atgaccctca gacgtgggtt ctcttaatat
     1681 tatattttgt cgctgggatg tactttagtt catctgaaac ggcgtttatg actgcgaacc
     1741 gctttaaaat aagagccaaa gcagaagaag gtagcgtgag cgctaaactt gctgcttggg
     1801 ttataaatag actagaaaat tcaattattg ctgtcgttgt gtttaataat ttagcgcact
     1861 tactttttac tgcgctttta actgttgtcc ttgttaatca gtacggcaat gacactggtg
     1921 aattacttgc aactttaatt agtgtgccgc tgatgttttt atttgcggaa acgattccaa
     1981 aagtattagg acgtcgctat agtgaacaat acgtattaat tagcgcttat cttttagtgc
     2041 catttattat tattagctat cctgtcgcgc tttttttccg tggtattgtc tgggttgtta
     2101 aaaaactttt caaagccaca gaagataata ttttcaccgc tgatgatttt gagcaagtca
     2161 ttgaaacaat tgaagagcac gggattatta atgaagacgc tagtgatatt attatctcct
     2221 cccttgaatt tagtgaaaca gtggtaaaag atgttatcac accgaaaaac gcgattgttg
     2281 cctatgacat tagtaaattt aataaagatt catttaaaga tttcgtgcta tcaacacatt
     2341 tttcaagaat tccgctgtat gacggttcac ttgataaaat tgttggcgtt attttagtgc
     2401 gggaatatat taaacattat cagaagaaac caaatattaa cccgcgcaca attatggcac
     2461 ggccatatta tgttaattat aagattacac ttaccaaaat ggttgaaggc tttaagaaga
     2521 acaacacgca tattgctttt gtcgtcgatg ataaaaagaa actacttggt atggttacaa
     2581 tggaagacgt ccttgaagaa ttagtcggcc aaattgcgga aacaagtggt actgttaaag
     2641 gagcaagcgt taatgggtaa tgtaattaga tttacaattg ttattctttt atttgcgctt
     2701 agtttctttt tctcgagttc agagagtgct ttttccacga ttaatcggct tcgtgtggaa
     2761 cgtgatgtgg aagacggtaa aaagcgtagc aaaacactgc tgagaataat tacgaattat
     2821 gagaatactg tccaaacaat attacttggt aacaattttg taaatattgc agttaccgcc
     2881 cttgtgacag ggatggcgct agcctatgat aatg
//
LOCUS       DAOSEX010000087         2899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_32675_length_2899_cov_2.338256, whole genome shotgun sequence.
ACCESSION   DAOSEX010000087 DAOSEX010000000
VERSION     DAOSEX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32675_length_2899_cov_2.338256"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1788)
                     /gene="priA"
                     /locus_tag="PK612_02250"
     CDS             complement(<1..1788)
                     /gene="priA"
                     /locus_tag="PK612_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOH94965.1"
                     /translation="MQLIEVFTQYSKLNRPFTYYYDGDDLEVGVRVLIDFNNREIVGY
                     VAAVKSTKETIETASKRLGFELAKITKILDEKPLLTNELMELSENVTSYYLTNRISVL
                     NAMLPPSLRPSKRSLSAPKIAMQKKVSFNPEFTDFDKLTDKQSSLLSEIMTHGSVLQT
                     SKNRTQIMQLLNKGAVIVEEIEKYRLRYEDYPREDPHEITNLQKEAVKSILEDYKETF
                     LLHGITGSGKTEIYLALAAHYLAQNKQVIMLVPEIALTGTMLANFYRRFGKKIAILHS
                     ELTPAEKYDEYRRISAGEVDIVVGARSAIFAPLQNIGLIILDEEHAETYKQDVAPFYH
                     ALTVAQMRAKSFNAKVLVGSATPSIETMARARKEVYGYYYLGERINLQPLPETTVVNM
                     LDYKNVTTTSPYISKLLATEIRKAIDAKTQVILLVNRRGYATFVSCRECGESKKCPAC
                     AISLTYHKVGNNLKCHHCGLSFPKNDPCIKCGSTNFSYTGFATQKAEEDIQKMFPDAR
                     ILRLDSDVSRKDKTPQVVLNKFMNHKADILIGTQMVSKGHDFPLVSLVGVIGTDANLS
                     IPSFRSSERTFQLITQAIGRSGREKIKGKA"
     gene            complement(1791..2363)
                     /gene="gmk"
                     /locus_tag="PK612_02255"
     CDS             complement(1791..2363)
                     /gene="gmk"
                     /locus_tag="PK612_02255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOH94966.1"
                     /translation="MRQGFIVLISGPSGVGKGTLIHSLLADQTLNLSFSVSMTTRKPR
                     PNEKDGIHYFFVSHETFLKNIEENNFLEHAQFVGNRYGTPRDYVEKLLNEGKNVLIEI
                     EVDGARQLLKNVDSEKVLSLYIMPPSVQELVRRLRLRGSEDEATIQRRAERFKEEIKY
                     KDQYQHIIINDELETCINDVKALIREKMNI"
     gene            2452..>2899
                     /locus_tag="PK612_02260"
     CDS             2452..>2899
                     /locus_tag="PK612_02260"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HOH94967.1"
                     /translation="MQRLFPNDLQQLINANKHFEGLFLVRIRQLSLVDFVFEFSHGNN
                     LFVSLNSPTPFIVFNADLSDFPEGGELHSFAINLRQFLGGKLVAMNQPNDDMILSLVF
                     EQRNNVLELEEVTLFIELIPRHPQAVLVNNKGRILAAYRYHQERAKD"
BASE COUNT          867 a          534 c          469 g         1029 t
ORIGIN      
        1 cgccttccct tttatttttt cgcgacctga tctaccaatc gcttgggtga ttagctgaaa
       61 tgttcgttca ctacttctaa atgagggtat tgataaatta gcatcagtgc caattactcc
      121 cactaaacta actaatggga aatcatgacc tttcgaaacc atttgcgtac caattaaaat
      181 atctgctttg tggttcataa atttgtttaa cactacttgt ggtgttttat ctttacgact
      241 cacatcacta tcaagccgca agatccgtgc atcaggaaac attttttgaa tatcttcttc
      301 agctttttgt gtggcaaatc cagtatatga gaaattagtg ctaccacatt tgatgcaagg
      361 atcattttta gggaaagaaa ggccacagtg atggcatttt aggttatttc ctactttatg
      421 ataggtgagt gatatcgcac aggcagggca ctttttagat tcgccacatt cacgacaact
      481 tacaaaagtg gcgtaccccc gccggtttac taataaaatc acttgtgtct tggcatcaat
      541 agcttttcta atttcggtcg ctaataactt agaaatatat ggtgaagtag tggtaacatt
      601 tttatagtct aacatattaa caactgtcgt ttctggtaag ggctgcaagt taattctttc
      661 acctaggtaa taataaccat aaacttcttt tctagcgcgc gccatcgttt caatgcttgg
      721 tgttgcacta ccaactaata ctttagcgtt aaagcttttc gcccgcattt gcgctacagt
      781 taaggcgtgg taaaaaggtg caacatcttg cttataagtt tcagcatgct cttcatctaa
      841 tataataagc ccaatatttt gtaatggggc aaaaatagca ctcctggcac caacaacaat
      901 atcaacttca ccagcgctga tgcgacggta ttcatcgtat ttttcagctg gtgtaagttc
      961 actatgaagg atagcaattt tcttaccaaa tcttcgataa aaattagcaa gcattgttcc
     1021 agttaaagca atttctggta cgagcattat aacttgttta ttttgtgcta aatagtgagc
     1081 ggcaagggca agatatattt ctgttttacc acttccggta ataccatgga gcaaaaatgt
     1141 ctctttataa tcttctaaaa tggattttac tgcttctttt tgcaaattgg ttatttcatg
     1201 tggatcttca cgcggataat cttcgtagcg taatctgtat ttttcaatct cttccacaat
     1261 tacagcacct ttatttaaca gctgcattat ttgcgttcta ttttttgaag tttgtaaaac
     1321 actaccatgc gtcattattt cacttaataa tgaactttgt ttatctgtta acttatcaaa
     1381 atcagtaaat tcaggattaa aacttacttt tttttgcatt gcaatctttg gcgcacttaa
     1441 actacgttta ctaggccgta gtgagggtgg taacatcgca tttaaaacac taatccgatt
     1501 tgttaagtaa taactagtaa cattttcact caattccatt aattcgttag ttaagagtgg
     1561 tttttcatcg agaatctttg ttattttagc aagctcaaag cctaggcgtt tacttgccgt
     1621 ttcaattgtc tctttggtgc ttttgacggc cgctacataa ccaacaattt cacgattatt
     1681 aaaatcgatg agaactctta ctccaacttc taaatcatcg ccatcataat aatatgtaaa
     1741 aggacgattt aattttgaat attgtgtaaa aacttcaatg agttgcatat ttaaatattc
     1801 atcttctccc gaattaatgc cttaacatca ttaatacacg tttctaactc atcatttatg
     1861 ataatgtgct gatattggtc tttatattta atttcttctt taaatctttc agcgcgacgc
     1921 tgtattgttg cttcgtcttc cgagccacgt aagcgtaagc gtcttactaa ttcttgaact
     1981 gaagggggca ttatataaag ggaaagaact ttttcactat caacattttt taataactga
     2041 cgggcaccat caacttcaat ttcaattaaa acatttttgc cttcattcaa taatttttca
     2101 acatagtcgc gaggcgttcc atagcgattt ccaacaaact gagcatgctc taaaaaattg
     2161 ttttcctcga tgtttttcaa aaatgtttca tgactaacaa aaaaataatg aatcccatct
     2221 ttttcattag gacgtggttt tcttgtagtc attgaaacgc taaaacttaa gttcaatgtt
     2281 tgatcggcaa gtaaactatg aataagtgtt cctttgccta cacctgatgg accacttatt
     2341 aaaacaataa agccttgtct catatatttt ccccttgatt ttccttaata aattgtatct
     2401 tatttattaa taaataacaa acttgtcaca atcttgttgt agaataaaag catgcaaaga
     2461 ctttttccaa atgatttaca acaattaatt aacgctaata aacattttga aggtttattt
     2521 ctagtgcgaa ttcgtcaatt aagtttggtt gattttgtat ttgaatttag tcatggtaat
     2581 aacttatttg tctcattaaa tagtccgacg ccttttattg tgtttaacgc tgatttaagt
     2641 gatttccccg agggcggaga attacatagt tttgccataa atttaagaca gtttttaggt
     2701 ggtaaattag tggctatgaa tcaacctaat gatgatatga ttttatcact agttttcgag
     2761 caaagaaaca atgttttgga acttgaagaa gtcacacttt ttattgaatt aattccgcgt
     2821 catccccaag ctgtattagt gaataataaa ggacgtattt tagcagcgta tcgctatcat
     2881 caagaacgcg caaaagatg
//
LOCUS       DAOSEX010000088         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_32687_length_2898_cov_3.611678, whole genome shotgun sequence.
ACCESSION   DAOSEX010000088 DAOSEX010000000
VERSION     DAOSEX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32687_length_2898_cov_3.611678"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1150)
                     /locus_tag="PK612_02265"
     CDS             complement(59..1150)
                     /locus_tag="PK612_02265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH94968.1"
                     /translation="MYLTTARHPHDYEMFLKKYKKAPNPSNQNFHNKLINILRSKFPL
                     GAFSTRPMEKNIFLTQSKSDYFYYPGYINTPVLKRLGIITAGLQYYEARTPKVIFVDT
                     LNVTLLELARRLKKRHNVKVIGIVTDNPINITGASKNYSQDVFKKSLVCDGFISLTQG
                     LLRLFNPKQKPAIIVPGFIEEAESFTNKKEKYAFFAGALYPKYGVDALIKTFRKLNPP
                     FKLVIAGHGPLADDLLENKHPNIEFLGQVCPPVAFRLAQRAYVNINPRPIDEQVDLYS
                     VPSKVIDYINSGSVTISTENTEIRNLVGDNIYFIKDAEPHTILEALNAINKNYNYYKQ
                     RAAEAKKALVKALDNKVLINKIEDLINKI"
     gene            complement(1150..2238)
                     /locus_tag="PK612_02270"
     CDS             complement(1150..2238)
                     /locus_tag="PK612_02270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOH94969.1"
                     /translation="MKILIVFNHPAPYKVALFNGLSAKHDVHVIFERFKASNRNQSFY
                     NESNYKFTLHKIKGVSLGDENHLSFGVVRHLKKHKYDLIIMNGYSTLTEMLALRYLKR
                     KKIPYAFYINGGFANEKECKLKKKIKTYFISGAEAYFSPAKKANEYLTYYGAPADKIY
                     NYPYSTVYEKDVLEVKTPTATKVLYWKEKGIEATNFTICITSFIKRKNNAALIKAWKE
                     IPKDYALILVGEGYEEPMYREIIANEKLTNVHLLPFAPKHLVFEYLSHSDNAVYISNY
                     DIYGHVINEALAHGLNVLTNTKMVAAIDLIRDGQNGLIAYPEDDLVSRILLLLATDFF
                     NEAIATAKENTIEESVRVHLDILEKICE"
     gene            2304..>2898
                     /locus_tag="PK612_02275"
     CDS             2304..>2898
                     /locus_tag="PK612_02275"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOH94970.1"
                     /translation="MNEIKTWEDFWEREKDSLYLQNLKVFLNAQDPLTTYPARDKIFN
                     AFKLTPLEDVKVVIIGQDPYHQPGQAMGLAFSVPSNVPLPPSLRNIFIEMADDVGGPV
                     RTDGDLTYLAKQGVFLINTILTVERSKPLSHNISEYKTLIVKVLEELNKRPQPIVFIL
                     WGNYARSFKKYINGPNKLVIESNHPSPLSANRGGFFGT"
BASE COUNT          928 a          494 c          481 g          995 t
ORIGIN      
        1 aatcatgtgg ccacatgatt ttattgtgtg tctacattta gggtatcagt acaatttact
       61 agattttatt aattaaatct tcaattttat taataagaac tttgttatca agtgctttta
      121 ctaaagcttt tttagcttca gcggcccttt gtttataata gttataattt ttgttaatag
      181 cgttaagtgc ttcaagaatt gtgtgtggtt cagcgtcttt tataaagtaa atattatccc
      241 ctaccaagtt tctaatttcc gtgttttcag tggaaatagt aacacttccg ctattgatgt
      301 aatcaataac tttacttggt actgaataga gatcaacctg ttcatctatt ggccgcggat
      361 taatattgac atacgccctt tgcgctaaac gaaaggcaac aggcggacaa acttgaccaa
      421 ggaactcaat attaggatgc ttattttcaa gtaaatcatc cgcaagcggg ccgtgtcccg
      481 ctatgactaa tttaaaaggc ggattaagtt tacggaatgt tttaattaaa gcatcaactc
      541 cgtattttgg gtataatgct ccagcaaaaa aggcgtattt ttccttttta ttggtgaaac
      601 tttcagcctc ttcaataaag cccggaacaa taattgctgg tttttgtttc ggattaaaaa
      661 ggcgtaagag tccttgagtt aaactaatga agccatcaca cacgagactc ttcttaaata
      721 catcttggct atagttttta cttgcaccag taatatttat tgggttatca gtaacgatcc
      781 caataacttt aacattatgt cttttcttta atctacgcgc taattcaaga agcgtcacat
      841 ttaatgtatc aacaaatata acttttggtg ttcgtgcttc atagtattgt aatccagcag
      901 taataatgcc aagccgttta agaacgggag tattaatata accaggataa taaaaataat
      961 cacttttact ttgcgttaag aaaatattct tttccatcgg gcgtgtacta aaggcaccaa
     1021 gcggaaattt actgcgtaaa atattaatta atttattatg aaagttttga ttacttggat
     1081 taggagcttt tttatatttt tttagaaaca tctcgtaatc atgcggatga cgagcagttg
     1141 ttaaatacat tattcgcata ttttctccaa tatatctaag tgcactcgca ctgattcttc
     1201 aattgtgttt tcttttgcgg tagcgattgc ttcattaaag aagtcagtcg caagcagtag
     1261 taatattctg cttactaaat cgtcttctgg ataagcaatt aaaccgtttt gtccgtcacg
     1321 aattaagtca attgcggcca ccatttttgt atttgttaaa acattaagcc cgtgcgctag
     1381 tgcttcatta atgacatgac cataaatatc gtaattacta atataaacag cattatcact
     1441 atgtgataaa tattcaaata caaggtgttt aggcgcaaag ggaagcagat gtacatttgt
     1501 taatttctca tttgcaataa tttcgcgata catcggttct tcatagcctt cacctactag
     1561 aatgagggca tagtcttttg gtatttcttt ccacgcttta ataagcgctg cattattttt
     1621 acgtttaata aaactagtta tacaaatcgt aaaattagtt gcttcaattc ctttttcttt
     1681 ccaatacaaa actttagtag cggttggtgt tttaacttct aaaacatctt tttcataaac
     1741 tgtggagtaa ggataattat aaatcttatc agcgggcgct ccgtaataag ttaagtactc
     1801 atttgccttt ttagcaggac taaagtaggc ttctgcgccg ctaataaaat aagttttaat
     1861 tttcttttta agcttacatt ctttctcatt agcgaaaccg ccattaatat aaaaagcata
     1921 aggaattttt ttacgcttta aataacgaag tgcaagcatt tctgttaatg ttgagtaacc
     1981 attcataata attaagtcgt atttatgctt ctttaagtga cgtactacac caaacgaaag
     2041 atgattttcg tcgccaagag agacaccctt tattttatgg agggtaaatt tataatttga
     2101 ttcattgtaa aaactttgat tacgattgct tgctttaaag cgttcaaaaa taacatgcac
     2161 atcatgtttt gcacttaaac cattaaagag tgcgacttta tatggagcag gatgattaaa
     2221 cacaattaat attttcatat gtataaatta tacaacaact taagccaaga aacattgcaa
     2281 agcaatgatg atataattat tttatgaatg aaattaaaac ttgggaagac ttttgggaac
     2341 gcgaaaaaga ctcactttat ttacaaaatt taaaagtttt tcttaatgcc caagaccccc
     2401 taacaacata tccagcgcgc gataaaattt ttaacgcttt taaattaaca ccgctagaag
     2461 atgtcaaagt tgtgattatt ggccaagatc cttatcacca accaggccaa gcaatgggac
     2521 tagcttttag tgtgccttca aatgtgccgc tcccgccaag cttaagaaat atatttattg
     2581 aaatggctga tgatgtgggt ggaccagttc gcacggacgg agatttaact tatttagcta
     2641 aacaaggcgt atttcttatt aataccattt taactgttga aagaagtaaa ccactttcac
     2701 ataacatttc ggaatataaa acgttaatcg ttaaagtact tgaagaatta aataagagac
     2761 cccaaccgat tgtctttatc ttatggggga attatgcaag gtcatttaaa aaatatataa
     2821 atggtcctaa taaattagtg attgagtcaa atcacccttc gccattatca gcgaatagag
     2881 gcggcttttt tggaacac
//
LOCUS       DAOSEX010000089         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_32785_length_2892_cov_3.156151, whole genome shotgun sequence.
ACCESSION   DAOSEX010000089 DAOSEX010000000
VERSION     DAOSEX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32785_length_2892_cov_3.156151"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PK612_02280"
     CDS             <1..425
                     /locus_tag="PK612_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HOH94971.1"
                     /translation="LGIINYLKDEGVEIRGKNVVIIGRSNIVGKPAAMLFLEEDGNVT
                     VLHSKTSREDLKNYVANADIMVVAVGRKHFINDEHTLKNSAVLVDVGINRDEDGKLTG
                     DIKPGRDVYLQTPVPGGVGLLTRLTLLENVMEAYLHGI"
     gene            415..1011
                     /locus_tag="PK612_02285"
     CDS             415..1011
                     /locus_tag="PK612_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94972.1"
                     /translation="MEFKIENSVVYGLERAVKASGNPMRTEIEGGALHEKDIKRAVLL
                     GTTKGGEGHDNFLKGILVHVDITAPFYWWKEAQRYHWFDFISSQSTMHMLLKFKIDER
                     CVPETDKRVFPILEELVNDYNAISEEDKDAKVKAWRKIVATLPSGFCLAATMTTNYQQ
                     LKTMYFQRRNHKLKEWHTFCDWCLTLPHFKEFVIKEGK"
     gene            1016..2242
                     /locus_tag="PK612_02290"
     CDS             1016..2242
                     /locus_tag="PK612_02290"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOH94973.1"
                     /translation="MKKSDLKKYARLIIRVGANVQKGQDVIITAELEQREFVLMLIEE
                     AYRAKANRVTLDWIDDDISKLHNKYRTVKSMSHFEKWELERYQYYVDKNPVRIYITSE
                     DPDGEKGINQEKRAQVMQNIYPVIKPYRDEMENKYQWTIAGAAGTKWAKKLFPDLTAK
                     QARNKLWDAILTTSRAYGDPIANWNEHNRSMSEKSRKLNELGLISLHYTSKNGTNFKV
                     GLIPNALFLAGGETTLGSNIFYNPNIPTEECFTTPKRGEAEGKVVATMPLSYQGELID
                     GFWFEFKDGKVVDFGAKQNEELLKQMLDMDEGARYLGEVALVPKESPINQLGLLFYST
                     LYDENASCHLALGLGFSNCIRDFEKYSLEELREMGINNSMIHVDFMIGADDLRIVGTT
                     KYGQEVVIFNEGTWAI"
     gene            2242..>2892
                     /locus_tag="PK612_02295"
     CDS             2242..>2892
                     /locus_tag="PK612_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94974.1"
                     /translation="MAKNKRKCRPLCFILKAVFVLLGVVVLVLGGYIGYVSLSYYRIG
                     DTSLQVLNNQTAKLSVADIGTKEFKITTYNVGFGAYDRDYSFFMDTSVFKDEYIHSQG
                     NKSNTGDRARGTSKAQVLRNINGAYDTIAALGDLDFMLYQEVDTSSTKSYKVNQYEIG
                     NKKHADYATIYGINYHSAYLMYPLHEPIGKSNSGISTYSKYHIAKAERREFEITDAF"
BASE COUNT          963 a          453 c          623 g          853 t
ORIGIN      
        1 cactaggtat cattaattat ttaaaagatg aaggtgttga aatccgcggg aagaatgtcg
       61 tgattattgg gcgcagcaat attgttggta aacctgctgc tatgctcttt ttagaagaag
      121 acggtaatgt aacagtatta cattcaaaga caagtagaga agaccttaaa aattatgtcg
      181 ctaatgcgga tattatggtt gtcgccgtag ggcgtaaaca ctttattaat gacgaacata
      241 cacttaaaaa tagtgcagtt cttgtagatg ttgggattaa tcgtgatgaa gacggaaaat
      301 taactggtga cattaaacca gggcgtgatg tttatttaca aactccagtg ccaggtggcg
      361 ttgggttatt aactagatta actttattag aaaatgtaat ggaggcttat ttacatggaa
      421 tttaaaattg aaaacagtgt tgtctacggt ttagagcgcg ccgttaaagc gagcggaaat
      481 ccgatgcgga ccgaaattga aggtggcgcg ctacatgaaa aagatatcaa gcgagcagtg
      541 ctactcggaa cgactaaagg tggcgaaggt catgataatt ttttaaaagg gatcttagtg
      601 catgttgata ttaccgctcc tttttactgg tggaaagaag cacaacgcta ccactggttt
      661 gactttatta gtagtcaatc aacaatgcat atgctgttaa aatttaagat tgatgaacgc
      721 tgtgtccccg aaactgataa gcgtgttttc cctattttag aagaactagt aaatgattat
      781 aacgccataa gtgaagaaga taaagatgca aaagttaagg cgtggcgtaa gattgttgcg
      841 actttaccaa gcggtttttg tcttgcggcg acaatgacga cgaactatca acaacttaaa
      901 acgatgtact tccaacgtcg taatcacaaa cttaaagagt ggcatacttt ttgtgattgg
      961 tgtttaactt tgccacactt taaagagttt gtaattaaag aaggaaaata aatgtatgaa
     1021 aaaaagcgat cttaaaaaat atgcgcggct tattattaga gtcggagcga atgtacaaaa
     1081 aggacaagat gttattataa ctgcggaact tgaacaacgc gaatttgtgt taatgcttat
     1141 tgaagaggct tatcgcgcta aagcaaaccg cgtgacgctt gactggattg atgacgacat
     1201 tagtaaactc cataataaat accgcactgt taaatcaatg tcacattttg aaaagtggga
     1261 gttagaacgt tatcaatatt atgttgataa gaatcccgtt agaatttata ttacaagcga
     1321 agaccctgat ggtgaaaaag ggattaatca agaaaagcgt gcgcaagtaa tgcaaaatat
     1381 ttaccccgtt attaaacctt atcgtgatga gatggaaaat aagtatcaat ggacaattgc
     1441 cggtgctgct ggtacaaagt gggcgaagaa attatttcca gacttaaccg caaaacaagc
     1501 ccgcaataaa ttatgggacg caatattaac gacgagccgt gcttatggcg acccgattgc
     1561 taattggaat gaacataatc gaagtatgag cgaaaagtct cgcaagctta acgaattagg
     1621 actcatttca ctacattaca ctagcaaaaa cggcactaat tttaaagttg gccttattcc
     1681 taatgcactc tttttagcgg gtggtgaaac aactttaggt agtaatattt tctataatcc
     1741 taatattccg actgaagaat gttttacaac accaaagcgc ggagaagctg aaggcaaggt
     1801 cgtagcgacg atgccgctta gttatcaagg tgaattaatt gatggctttt ggtttgagtt
     1861 taaagacggt aaagttgtcg actttggcgc taaacaaaat gaagaattat taaaacaaat
     1921 gttagatatg gatgaaggcg ctcgttatct tggtgaagta gcgctcgtcc cgaaagaatc
     1981 accaattaat caattagggc tcttatttta ttcaacgctt tatgatgaaa atgcttcgtg
     2041 ccacttagcg ctaggtcttg ggtttagtaa ttgtattcgt gattttgaaa agtactctct
     2101 tgaagaactt cgtgaaatgg gaattaataa ctcaatgatt catgttgatt ttatgattgg
     2161 cgctgatgat ttacggattg tcggtacaac aaaatacggt caagaagtag taatctttaa
     2221 cgaaggaacc tgggcaattt aatggcaaaa aataaaagaa aatgtagacc gttatgcttt
     2281 atcttaaagg cagtgtttgt gcttttaggt gtcgtggtgc ttgtcttagg tggttatata
     2341 ggttatgtta gcctctctta ctaccgcatt ggtgacacta gtcttcaagt attaaacaat
     2401 caaacagcga aattaagtgt cgctgatatt ggtacaaaag aatttaagat tacaacatat
     2461 aatgttggtt ttggggcata tgacagagat tacagtttct ttatggacac gagtgttttt
     2521 aaagatgaat atattcattc gcaaggtaat aaaagtaaca ctggtgatcg tgcgcgtggt
     2581 acaagtaaag cacaagtgct aaggaatatt aacggtgctt atgacacaat tgcagcttta
     2641 ggtgatttag actttatgct ttatcaagaa gttgacacaa gttcaacaaa gtcttataaa
     2701 gttaatcaat atgaaattgg taataaaaaa cacgctgatt acgcaacaat ttacggtatt
     2761 aactatcact ctgcttattt aatgtaccca ctgcatgaac caattggtaa atcaaattca
     2821 ggtatttcga catactctaa atatcatatc gctaaagctg aacgccgtga atttgaaatt
     2881 acggatgcct tc
//
LOCUS       DAOSEX010000090         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_33268_length_2864_cov_2.480954, whole genome shotgun sequence.
ACCESSION   DAOSEX010000090 DAOSEX010000000
VERSION     DAOSEX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33268_length_2864_cov_2.480954"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /gene="ricT"
                     /locus_tag="PK612_02300"
     CDS             <1..807
                     /gene="ricT"
                     /locus_tag="PK612_02300"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOH94975.1"
                     /translation="ISIIYNDGGKSYCFGTNESSLDIGDYVLVESSQGIAVAKVVATP
                     QTMDKYSSPLELKDIVRRANEHEIRQHQDNLKDAELAMAFCQKQVERLGLKMNLLTSE
                     YTFDRSKINITYSAEERVDFRELVKILGSHLKTRVELRQIGSRDRAKLIGGLGQCGFP
                     LCCHTFLTEFDGISINRAKNQMLPLNIPKLSGHCGKLLCCLKYEDDYYTEAKKEFPNV
                     GSTYTRGNTLYQLTSFNIVNRTLRLDYEDGFVIVPLDELKRDYKVKHEKK"
     gene            794..1636
                     /gene="rsmI"
                     /locus_tag="PK612_02305"
     CDS             794..1636
                     /gene="rsmI"
                     /locus_tag="PK612_02305"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOH94976.1"
                     /translation="MKRNKSFSGTPFYLVATPIGNLEEFSPRAVNVLRSVDFICAEDT
                     RTSGVLLNHFGIKKELISVHKFNEQSKLELILNKLKNGEKGAFISDAGYPLISDPGHP
                     LVKALIANDFSVSVINGPSALLPALIGSALDTETFTFIGFLKGNNKDVLNTLKRYKSY
                     KDTLIIYEAPHRVVKTIATIYTVFAERKLVIARELSKLHEEYIYTSLAEFSANPLTDL
                     KGEIVLVIEGNKDEENNDLADLLDKVELLISNENMSVKKATSVVALLYNVSKNKLYNA
                     YLKR"
     gene            complement(1626..>2864)
                     /locus_tag="PK612_02310"
     CDS             complement(1626..>2864)
                     /locus_tag="PK612_02310"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOH94977.1"
                     /translation="YWSNAAPSVAPYRNVPNTYEFMEELSTDKRFVYKMKEGFTLGFG
                     VDNVISYENRLSDGTYFDLIEKDRALSPIQNEELYLTSALLSYEDHYEVTRTYSDLVS
                     HDINAFPHQHYTRLSSTNGGLKVKYYEFTGRRYPYEAQTRTLGEVVLPTPQWVSENGN
                     EIISSLVPTELNGAHHVIEYTKKDGTNFVNGVNELGEQIGGEIIMRLPLSYYNSSTLY
                     YNFRYRVFLYDENYELITFDDHHTLPSSWTDWKTLRSFHTPKAVSKIALVPIGSRVTP
                     PYVHELFTYSQDALESIRQTSITNSLTDVKVTTNTATFKTNYPTRKFVVTTIPYDKGW
                     RVTNENNIKIPVYRVQGGFVGFVSDRGEMSYTMSFRPQYFNMGLVLTFSAGIATLLLE
                     LNVYFFKKYKKKKAEENINV"
BASE COUNT          911 a          498 c          532 g          923 t
ORIGIN      
        1 ataagtatta tatataacga tggtggcaag tcttattgtt ttggaacaaa tgaaagttct
       61 ttagatatag gcgattatgt tttagtagaa agcagtcaag gtatagccgt tgctaaagta
      121 gtggcaacgc cccaaacaat ggataaatat agtagcccat tagagttaaa ggatattgtt
      181 agacgcgcta atgagcatga aatccgtcaa catcaagata atctaaaaga tgctgaactt
      241 gcaatggcct tttgtcaaaa gcaagtcgaa cggcttggtc ttaaaatgaa tttacttact
      301 tcggaatata cttttgatcg tagtaagatt aatattactt actccgctga agaacgcgtt
      361 gactttagag agcttgtgaa aattcttggt tcacacttaa aaactagagt agaactacgc
      421 caaattggta gtagagaccg cgctaaacta attggtggtc ttgggcaatg cggcttcccg
      481 ctttgttgcc atactttctt aacagagttt gatggtattt cgattaatcg tgctaaaaac
      541 caaatgttac cgcttaacat tccaaaatta agtggccact gtggtaaatt actttgttgt
      601 ttaaagtatg aagatgatta ttatacggaa gcaaaaaaag aattccctaa tgttggttcg
      661 acttacactc gcggtaacac tttgtatcaa ctaacatcat ttaatatcgt taaccgtact
      721 cttcgtcttg attatgaaga tggttttgtg attgtgccct tagatgaatt aaaaagggat
      781 tataaagtta aacatgaaaa gaaataaatc ttttagcggt acaccatttt atttagtggc
      841 tacgccaatt ggcaatttag aagagttttc gccgcgcgct gttaatgtgc taagaagcgt
      901 tgattttatt tgcgctgaag ataccagaac aagcggtgtt cttttaaatc atttcggtat
      961 taaaaaggaa cttattagtg tccataaatt taatgagcaa agtaaattag agttaatctt
     1021 aaataaatta aaaaacggag aaaaaggcgc ctttattagt gatgccggtt atccgcttat
     1081 tagtgatcca ggacacccgt tagttaaagc gttaatcgct aacgatttta gtgttagtgt
     1141 tattaatggt cctagtgcac ttttaccagc gctgattggt agcgccttag atactgaaac
     1201 atttacattc atcggctttt taaaaggtaa taataaagat gtgctcaaca ctttaaagcg
     1261 ttataaatct tacaaagaca cattaataat ttacgaagct ccgcatcgtg ttgttaagac
     1321 aatagcgacg atttacacgg tttttgccga aagaaagctt gtaattgcgc gtgaattaag
     1381 taaattgcat gaagaatata tttatacatc actagcggaa tttagtgcta atccactgac
     1441 ggatttaaaa ggtgaaatcg ttttagtcat cgaaggcaat aaagatgaag aaaataatga
     1501 cttagcggac cttttagata aagtagaact attaattagt aatgaaaata tgagtgttaa
     1561 aaaggcgact agtgtcgtcg ccttgctcta taatgtttct aaaaataaac tatataacgc
     1621 ttatcttaaa cgttaatatt ttcttcggct tttttcttct tatatttctt aaagaagtac
     1681 acatttagtt ctagtaataa tgtagcaata cctgcagaga aagtaagcac taaacccata
     1741 ttaaagtatt gcggtctaaa cgacattgta taactcattt caccccgatc gctaacaaaa
     1801 ccaacaaatc caccttgtac ccgataaacg ggtattttta tattgttttc attagtgaca
     1861 cgccatccct tatcgtaagg aattgtggta acaacaaact tgcgcgtcgg ataattagtt
     1921 ttaaatgtcg cggtattcgt tgtgacctta acatcagtta gtgaattagt gattgatgtt
     1981 tgtcttatag attctagtgc atcttgtgaa taggtaaata attcatgaac atatggcgga
     2041 gtaacacgac tgccaattgg cactagcgca attttagata ccgcttttgg tgtgtggaaa
     2101 ctccgtaaag ttttccaatc tgtccatgaa ctcggtaaag tatgatgatc atcaaaagta
     2161 attaactcat aattttcatc atataaaaac acccgataac gaaagttata gtaaagtgta
     2221 ctgctgttgt aataagaaag cggtaatcgc ataattattt cgccgccaat ttgttcgcct
     2281 agttcattta caccattcac aaaatttgtg ccatctttct tcgtatattc aatgacatga
     2341 tgtgccccat ttaattctgt tggcactaag ctacttatta tttcgttccc gttttcactg
     2401 acccattggg gtgtcggtag cacgacttca cctaatgttc tcgtttgtgc ttcataggga
     2461 tagcggcggc cagtaaattc gtaatacttt acctttaagc ccccgtttgt gctactaagc
     2521 cgcgtgtaat gttgatgcgg gaaagcgtta atatcatgac ttactaaatc actataagtc
     2581 cttgttactt cataatgatc ttcgtaacta agaagcgcac ttgttaaata gagctcttca
     2641 ttttgaatag gggaaagggc gcgatctttt tcaattaagt caaaataagt accgtcactc
     2701 agcctatttt cataacttat aacattatca actccaaaac caagcgtaaa accttctttc
     2761 atcttgtaga caaagcgttt atcagtgctt aactcttcca taaattcata agtgtttgga
     2821 acattacggt aaggcgctac gcttggcgca gcattactcc aata
//
LOCUS       DAOSEX010000091         2848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_33522_length_2848_cov_2.866094, whole genome shotgun sequence.
ACCESSION   DAOSEX010000091 DAOSEX010000000
VERSION     DAOSEX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33522_length_2848_cov_2.866094"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..662)
                     /locus_tag="PK612_02315"
     CDS             complement(123..662)
                     /locus_tag="PK612_02315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOH94978.1"
                     /translation="MYENERFLRISDTASGFTDHRLKKQYFALFDWYKRNLEYEMFLA
                     SNKLRYKINQGEVYEIDFGRNVGSELNERHYAVVLHHSDVEAQNIVVVPLTTKIHFSY
                     GEAIDLGYLPEVKTTEKSYAKISQIRTVDKARIYLRPIIHTEDNKPCRHTYGPVTKLT
                     ADQFRLVIEGLNKLLNNQL"
     gene            complement(731..1939)
                     /locus_tag="PK612_02320"
     CDS             complement(731..1939)
                     /locus_tag="PK612_02320"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOH94979.1"
                     /translation="MKNKKFKRVFVVVMDSVGVGAMPDAHLYGDAGSHTLKHTAESKK
                     NGLHVPNLEKYGLGNLDDIKGVKKVKNHPNSYVMKMLEASTGKDTMTGHWEFMGIHTE
                     KPFKTFTDTGFPQALIDELSAKTGYKIIGNYSASGTEIIKELGAEQMRDNSLIVYTSA
                     DSVLQIAAHEEVTGLDELYRCCEIAREICLRPEYLLGRIIARPYIGSDPSNFKRTANR
                     HDYALSPPEKTALNILDDNGITVSAIGKINDIYNGFGITQAQKTISNEDGMDKTIAQA
                     KANEFNGLIFTNLVEFDSEYGHRRNPVGYADAIERFDKRLGELMEVVTEDDLVLLTAD
                     HGNDPTWHGTDHTREMVPLIAISKAFTKGRVLEHRKSFADIGATILANYNLKPTPTLI
                     GEVIDELLTE"
     gene            complement(2011..>2848)
                     /locus_tag="PK612_02325"
     CDS             complement(2011..>2848)
                     /locus_tag="PK612_02325"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOH94980.1"
                     /translation="FLLLEKNLANTTVSAYLDDINLFHTTFNFTVTEEISPMHVSDFI
                     NLESQKGLSASTIMRRLSSIYNYLLFLKDEGDYKYELKRIPGPAVTKTLPTYLTNDEV
                     DRLLSMPNLNTHRGVRDKAMLEVMYASGLRVSELLSLKREEVNFNRGIIKIRGKGGKE
                     RIIPIGEFALEYLADYINNVRFHYEKSDKRTIFLNTNGKPLSRQYFFKMIKAYAQEAE
                     ITANVSPHTLRHAFATHLLENGADLRAVQAMLGHTNIATTQIYTHVSTKRIISAYDKI
                     MK"
BASE COUNT          836 a          540 c          477 g          995 t
ORIGIN      
        1 tatttattta attatttgat taattaatta tttaattatt tgattattta tttaattaaa
       61 ctttgcgcaa agcaaagagt tatccacaat ttcacacata ctaaataact actaattaat
      121 tactacaatt gattattgag caatttattc agtccttcaa tcactaatct aaattggtct
      181 gctgttaatt tcgttaccgg gccgtaagta tgtctgcacg gcttattatc ttcagtatga
      241 ataataggtc gaaggtaaat gcgcgcttta tcgaccgttc taatttggct aatctttgcg
      301 tagctttttt ctgttgtttt tacttctggt aaatatccta gatcaattgc ttccccataa
      361 ctaaaatgaa tcttagttgt taaaggaacg acgacaatat tttgtgcctc aacatcacta
      421 tgatgtaata cgacagcgta atgtctttcg ttaagttcgc ttccaacatt acggccaaaa
      481 tcaatctcat aaacctcccc ttgattaatc ttatagcgga gtttattact tgcgagaaac
      541 atttcatact ctaaattgcg tttataccaa tcaaagagcg cgaaatattg ctttttgagt
      601 ctgtggtcgg taaaaccact tgcggtgtcg ctaatgcgca ggaaacgttc attttcgtac
      661 ataaaaaatc ctcactttcg atattatgat tataaataca atagaattta tcgttttata
      721 acaaaatgcg ttattcggtt agtaattcgt cgattacttc accaattaag gttggtgtcg
      781 gttttaaatt gtaattagcg aggattgtcg caccaatgtc ggcaaagctt ttacggtgtt
      841 caagcacacg ccctttagta aatgctttgc taatagcaat aagcggtacc atttcacggg
      901 tatggtcagt gccgtgccat gttggatcgt taccgtggtc agcagttaaa agcactaaat
      961 cgtcttcagt aacgacttcc attaattcac cgagacgttt atcaaagcgt tcaatggcat
     1021 ctgcatagcc tactggattg cgtctatgac cgtattcact atcaaattca acgagattag
     1081 tgaaaattaa accgttaaat tcattagcct tcgcttgggc aatcgtttta tccattccgt
     1141 cttcattact tatagtcttt tgtgcttgtg taatgccaaa accgttataa atatcattaa
     1201 ttttaccgat tgcactgacg gttatgccgt tatcatcaag aatattaagt gccgtttttt
     1261 ccggtggaga aagtgcataa tcatgccgat tagcggtccg tttaaagttg cttgggtcac
     1321 taccgatata aggacgagca ataattctac caagaaggta ttcaggccgt aaacaaattt
     1381 cacgggcaat ttcacaacag cgatataatt catcaaggcc tgttacttct tcgtgcgctg
     1441 ctatttgtaa aacactatcc gcggacgtat aaacaattaa gctattatca cgcatttgtt
     1501 ctgcaccgag ttctttaata atctccgtac cacttgctga ataattacca atgatcttat
     1561 aacctgtttt cgcacttaat tcatcaatta gtgcttgcgg gaaacctgta tcggtaaatg
     1621 ttttaaacgg tttttccgta tgaatcccca taaattccca gtggccagtc attgtatctt
     1681 tacccgtgct agcttctaac attttcatga catatgaatt agggtggttt ttaacctttt
     1741 taacaccttt aatatcatca agattaccta agccatattt ttctaaatta ggaacatgta
     1801 atccattctt tttactttcc gctgtatgtt ttaaggtatg actaccagcg tcaccatata
     1861 aatgtgcatc aggcatagca ccaacaccga cactatccat aacgacaaca aagacgcgtt
     1921 taaacttctt atttttcata atttaatcct ttcgatttct ttttttatta taacaaataa
     1981 tagtttgtgc tttattaatt taacacctta ttatttcata atcttgtcat atgcactgat
     2041 gatgcgttta gtggacacat gtgtatatat ttgtgttgta gcaatattag tgtggccgag
     2101 cattgcttgt acggctctta aatcggcgcc attttcgagt aagtgtgtgg caaaagcgtg
     2161 gcgtaatgta tgcggtgata cattagcggt tatttcagct tcttgcgcat aagctttaat
     2221 cattttaaag aaatattgac gacttaatgg tttaccatta gtgtttaaga aaatagtgcg
     2281 tttatcactt ttttcataat gaaagcgaac attatttata taatccgcta agtattcgag
     2341 ggcgaattca ccaattggga taatccgctc tttcccacct ttcccgcgaa ttttaatgat
     2401 gccacgatta aaatttactt cttcacgctt taaacttaaa agctcgctaa ctcgtaatcc
     2461 gctagcatac attacttcaa gcatcgcttt atcgcgaaca ccgcgatgag tgtttaagtt
     2521 tggcatactt aaaagtcgat caacttcatc attagtgaga tatgttggca atgttttagt
     2581 gactgctggt ccaggaatgc gtttaagttc atatttataa tcaccttcat cttttaaaaa
     2641 gagcaaataa ttataaatgc ttgatagtct ccgcattatt gttgatgcac taagtccttt
     2701 ttgactttct aaattaataa agtcactaac atgcattggc gaaatttctt cggtaactgt
     2761 gaagttaaag gtcgtatgaa agagattaat atcatctaaa taagcactta ccgtcgtatt
     2821 agcaaggttt ttctcaagta ataagaaa
//
LOCUS       DAOSEX010000092         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_33612_length_2843_cov_3.786944, whole genome shotgun sequence.
ACCESSION   DAOSEX010000092 DAOSEX010000000
VERSION     DAOSEX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33612_length_2843_cov_3.786944"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PK612_02330"
     CDS             <1..217
                     /locus_tag="PK612_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94981.1"
                     /translation="GSYAPLPLSQYAGNNNVFAFKRELGGETYNVYINLRNGGLTSLN
                     LPGTVILSLEGATKNTLPAWSVLVTKI"
     gene            complement(413..1687)
                     /locus_tag="PK612_02335"
     CDS             complement(413..1687)
                     /locus_tag="PK612_02335"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HOH94982.1"
                     /translation="MNYNVFLLRLGLDPSQFTNRMNEPIKTGNGWLYEVDQKSEGYKC
                     PHCHNDKLYIHDYNTIEINCRESSFIEDVLRVRKVRLRCKTCRKTFTPELRGVDAGFK
                     TSNQTLQLIYSEFTGDLTFAAIGKRYGLSTARVMQIFDDDIKYVPRRPMPEVLCLDEI
                     RFSKDKDQQYVCILYDFHNREIVDVIRNRRMPYLREYFNEINPIERAKVKFVISDMYD
                     AYATVARHYFKNAVHIVDLFHVIRLLTTAINQLRVRTMNMHAIKKTAEYNFMKQHWKL
                     FLCRTSKVPDKFYTHQKTNRRYHFDELLRYSLQLNNDLFTAYNILQDLFRYDLRFTYD
                     EALLFIQRISTNLADTSSPLLHAVGDSYHKWRFEIASAFSYTQNKIRYTNSIAENINN
                     QIKTITKAAYGYRNFDRFRKRVMLIITYKKKP"
     gene            2280..>2843
                     /locus_tag="PK612_02340"
     CDS             2280..>2843
                     /locus_tag="PK612_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH94983.1"
                     /translation="MKRKTYLRNQTIYQIYVRNHTAEGTFVALIKDLPRIKELGVDIL
                     YLLPIHPIGEIARKGELGSPYAIRDYYLINPEHGTLEDFQTFITLAHSYGFKVMIDIV
                     LHHTSPDAELLAEHPEFYYYNNGKLGNKVGDWSDIIDLDYNNRDLWDYMVEMLKYWAS
                     LGVDGYRADVAPLVPLAFWEYARQELDE"
BASE COUNT          925 a          504 c          547 g          867 t
ORIGIN      
        1 tggaagctat gcacccctac cactaagtca atatgctggt aacaataatg tttttgcttt
       61 caaacgggaa ttaggtggtg aaacatataa tgtttatatc aacttacgaa acggtgggtt
      121 aacaagtctt aacttaccag gtactgtcat tttatcgcta gaaggtgcta ctaaaaacac
      181 actaccagcg tggagtgtct tagttactaa aatttaacgc taaaaattat tcaatagatt
      241 agccaagatc cattcttggc ttttcttttt ttatttaagc ctcatttgac ttagatacat
      301 attaaagtta caatattatg gctcaattaa atagaacgtg gaaaatcgtg gaaaaagcga
      361 aaagaccccc aaaaactatt aaatagagcg tggacaagcc gtgacatcaa aatcacggct
      421 ttttcttata ggtaataatg agcatgacac gcttacgaaa acgatcgaaa ttcctatagc
      481 cataagcggc tttcgtgatg gttttaattt ggttgttaat gttttcagca attgaattag
      541 tgtaccttat tttgttttga gtataggaga aagcggacgc aatttcgaaa cgccatttgt
      601 gataagaatc accaacagcg tgtaataatg gtgaggaagt atctgctaga tttgtggaga
      661 ttctttgaat aaataggagc gcttcatcgt aagtaaaccg aaggtcataa cgaaagagat
      721 cctggaggat attataagca gtaaagagat cattatttag ctgaagcgaa tagcggagga
      781 gttcatcaaa gtgataacga cgatttgttt tttggtgcgt gtagaattta tcgggcactt
      841 tactcgtgcg acaaaggaaa agtttccagt gttgtttcat aaaattgtat tcagcggttt
      901 tcttgatggc gtgcatattc atcgtacgaa cccggagttg attgattgca gtcgttaata
      961 ggcgaatgac atgaaaaaga tcaacaatgt ggacggcgtt tttaaaataa tggcgggcaa
     1021 cagtcgcgta agcatcatac atatcggaga taacgaactt aactttagcg cgttctatgg
     1081 ggttaatttc attaaagtat tcgcgaagat aaggcatgcg acgattacga atcacatcaa
     1141 caatttcccg attatggaag tcatagagga tacaaacata ttgctgatct ttatcttttg
     1201 agaaacgaat ttcatcaaga caaaggacct caggcatcgg acggcgggga acatatttta
     1261 tatcatcatc aaagatttgc ataacccgtg ccgtacttaa tccgtagcgt tttccaatcg
     1321 cagcaaatgt tagatctccg gtgaattcac tataaattag ttggagcgtt tggttacttg
     1381 ttttaaatcc agcatcaacc ccacgaagct ctggagtaaa cgtcttacga caagttttac
     1441 accgtaatcg aacctttcta acgcggagca catcctcaat aaaactagat tctcgacaat
     1501 tgatttcgat ggtattgtag tcatggatat aaagtttatc attatggcag tgtggacact
     1561 tatatccctc acttttttga tcaacttcat agagccaacc attacccgtt ttaattggct
     1621 cattcatccg attagtaaac tgcgatggat caagacctaa acgtaaaaga aacacattat
     1681 aattcataaa aaactcctta attagttatg aattaagtat atataattat gtgttcaagt
     1741 gaccaaagct attaaatgca gcgtggaatt taaaaaaagt gtccacgttc tatttaattg
     1801 agccgttaaa aggaagagta cagtggttcg ctactataag gcggaagtca tcgagtatag
     1861 tgcttggtga cttttctctc aaataacgga aaaacaaccg cagaatctat tccaatttat
     1921 tagtaaacca ttttatcttt cataaacaca aggcgacaag tggcgaactt aaaaggtaaa
     1981 attcacactt gatttaggga cacgattact catactttcc accgtttacc caaagtggta
     2041 aaagattaac ccttttatga aagctcccaa actttctgtg agatacccac cgttaccaat
     2101 agtttccacg atttacccaa aaaggtcatt ttcttaccag tgacattttt taagagtaaa
     2161 aaacacttac cagttaagta atatatggca ccattattgt aaaaatctcg atatttagat
     2221 ccccctctag atatcgagtt ttttattact taaagctgtt tatttgctaa aatgaaagta
     2281 tgaaaagaaa aacatattta cgcaaccaaa caatttatca aatttatgtg cgtaatcaca
     2341 ccgcagaagg aacatttgtc gctttaatta aggacctacc aagaataaag gagcttggtg
     2401 tcgacatttt atacttgctg ccaattcacc ctattggtga aattgctcgt aaaggtgagc
     2461 taggaagtcc ttatgcgatt cgtgattact atttaattaa tccggaacac ggtacacttg
     2521 aagactttca aactttcatt actttagcgc attcatacgg ttttaaagta atgattgata
     2581 tcgtgcttca tcacactagt cctgatgccg agttattagc ggagcatcct gaattttatt
     2641 actataataa cggcaaactt ggcaacaaag ttggagactg gagtgatatt attgacttag
     2701 actataataa tcgtgactta tgggattata tggtagaaat gctcaaatac tgggctagtt
     2761 taggagtaga tggttatcgc gctgatgtgg cgccactcgt tccccttgca ttttgggaat
     2821 atgcacggca ggaactagat gaa
//
LOCUS       DAOSEX010000093         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_33650_length_2841_cov_3.133166, whole genome shotgun sequence.
ACCESSION   DAOSEX010000093 DAOSEX010000000
VERSION     DAOSEX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33650_length_2841_cov_3.133166"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..689
                     /locus_tag="PK612_02345"
     CDS             336..689
                     /locus_tag="PK612_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH94984.1"
                     /translation="MQVNGIIRLTKDPVVRKTQTGETVVNFDAAWNYDETGHFIRCTA
                     WGKIAELIGEYTEKGHRLYVIGKLIQSSFTTKNGEIRKTHEVEVSQIEFIENKPSSEK
                     PEAAVNEQEADDLPF"
     gene            732..1130
                     /locus_tag="PK612_02350"
     CDS             732..1130
                     /locus_tag="PK612_02350"
                     /inference="COORDINATES: protein motif:HMM:NF017663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HOH94985.1"
                     /translation="MRGKFVSAYYPKKVERKFNEYTKAIKGAFNALSVVERENISEIV
                     KIVPTGLKIALSVVFRLCPASSLSKKKKNALYGSEHNKKPDLDNLLKMIIDRMSGVFY
                     KDDNVIYEIHARKEYAEVPGIEITLEYKKE"
     gene            1377..1643
                     /locus_tag="PK612_02355"
     CDS             1377..1643
                     /locus_tag="PK612_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94986.1"
                     /translation="MLTKSTLQKIKDAYVGENGKGRIVFDPEGVERFNIAHLILTDIK
                     FRRSVIITISTLAGKLEVYSFKGEITIYEINAIQSILDNNKYEK"
     gene            1697..1861
                     /locus_tag="PK612_02360"
     CDS             1697..1861
                     /locus_tag="PK612_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH94987.1"
                     /translation="MKRKVYKQIEVAKMIGVHRNSVYRWVRDGKIKSVLVAGVRMIPA
                     SEIEKLTGEE"
     gene            1863..2597
                     /locus_tag="PK612_02365"
     CDS             1863..2597
                     /locus_tag="PK612_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94988.1"
                     /translation="MANRRMFAKTIIDSDAFLDMPPSSQVLYFHLAMRADDDGFINNP
                     KTIMRIVGAKDDDIKLLIAKKFIIPFDSGVVVIKHWRIHNYIQKDRYTETKYIEEKNQ
                     LIINEKNAYSLKQIGLLDDENDNVYILDTQVRLGKDRLELGKDRLELERDKNKATRFI
                     TPTIDEIKTFLNDQEYETDTDRYAVRFYNFYESKEWKVGKEKMKDWKAAIRGWLSRDN
                     IKKKEEVAVETIDDLNDPIWEEIKKL"
     gene            2611..>2841
                     /locus_tag="PK612_02370"
     CDS             2611..>2841
                     /locus_tag="PK612_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOH94989.1"
                     /translation="MITIYTVPSCSSCRKAKDWFNEWEIPFVEHDITKTPLEKEVLLD
                     ILYKAENGAEDIISTRSKLFKEKKIDFKTMTLS"
BASE COUNT         1064 a          445 c          623 g          709 t
ORIGIN      
        1 gaaatgaccg agttgatcga ctatgcaact gactatgcaa ttaaggtagg ggctgatgtc
       61 taaacgaacg aaagctctcg gcatatcagc gaaagtgcga aaagaggtct acgaaagaga
      121 tcggtggtgt gtgctatgcg gaaacccaca taatctgcaa tgtgctcact tcatctctcg
      181 aggtagtggg gggcttggca taccgcaaaa cctcgtaatg ctatgtgtaa attgccatta
      241 taaatacgat cagacatccg ctcgggatga gataagaaaa gaaataaaaa actatctatc
      301 gagatattat gaaataaatg aaaaggaatt gaaatatgca agtaaacgga attattagat
      361 tgacaaaaga cccggtggtg cgaaaaacac aaacgggcga aacagtggta aactttgatg
      421 ccgcgtggaa ctatgacgaa actgggcatt ttattcgctg cacagcgtgg ggaaaaattg
      481 ccgaacttat tggggagtac acggaaaaag gacatcgact ttatgtaatc ggaaaactca
      541 ttcaatcatc gttcacaact aaaaatgggg aaataagaaa aacgcatgag gttgaggtat
      601 cgcaaatcga gtttatcgaa aataaaccaa gtagtgaaaa gccggaagcc gctgttaatg
      661 aacaggaagc cgatgatctg ccgttttaat ttaaaatagc aagaattctt ctgccaagag
      721 caagacacgc attgcgaggg aaattcgtgt cagcgtatta tccaaaaaaa gtcgaacgaa
      781 agttcaacga atacacgaaa gcgataaagg gggcgtttaa cgccctttct gtcgttgagc
      841 gtgaaaatat aagcgagata gttaaaatcg tgcctacggg tcttaaaata gcgttaagcg
      901 tggtatttag gctttgccca gcgagtagtt tatcgaaaaa aaagaaaaac gccctatatg
      961 gcagtgaaca caataaaaaa ccagaccttg ataatttact taaaatgatt attgatcgaa
     1021 tgagcggggt attttataaa gatgataatg tgatctacga aattcacgca cgaaaggaat
     1081 atgcagaagt gcctggaatc gagataacgc ttgaatataa aaaagagtga atattcattt
     1141 taataattag cttattaata ggaggtaata ttcactcata gaaaggggtg ggaaaatgag
     1201 accaccatat atatatatta tacataaaaa ataacctttg cgatagttat gcggtaattt
     1261 atttcgccta ttaatttgcg tggttgaaaa taatttttat tttttttatc attatcgttg
     1321 ccatgtgttg ctaaatggtt tataatgtag acagaaagag aagaaaggag aataccatgt
     1381 taacaaaaag cacattacaa aagattaagg acgcttatgt aggcgagaac ggtaagggga
     1441 gaattgtatt cgaccccgag ggcgtagaac gctttaacat agctcactta atattaacgg
     1501 acattaagtt ccgccgctcc gttataatca cgatttcaac tttggcgggt aagcttgaag
     1561 tctattcgtt caaaggcgaa attacgattt atgaaattaa tgctatacaa tcaattctcg
     1621 ataataacaa atacgaaaaa taatacaatc aaggctcggc ggtgcctaaa aaccgcctac
     1681 aaaaaaagga ggaattatga aaagaaaagt ttacaaacaa attgaggtcg ctaaaatgat
     1741 cggcgtacac cgcaattcag tctatcgctg ggtgcgagat ggcaagatta aatcagtatt
     1801 agtcgccggt gtccgcatga tcccagcgag tgagattgaa aagctaacag gagaggaata
     1861 aaatggcaaa tagacggatg ttcgcgaaaa cgatcattga cagcgatgcc tttctcgata
     1921 tgccgccaag ttcacaagtt ttgtattttc accttgcgat gagagcggat gatgatggat
     1981 ttattaataa ccctaaaaca attatgcgaa ttgtgggggc aaaagatgat gacattaaac
     2041 tattaatcgc aaaaaaattt atcattccat tcgatagtgg cgtggtcgtg ataaaacact
     2101 ggcggattca taactatatt caaaaagacc gctatactga aacaaaatac atcgaggaaa
     2161 aaaaccaact tatcatcaat gaaaagaacg cttattcgct taaacaaatc ggattattag
     2221 atgatgaaaa cgacaatgta tacattttgg atacacaggt taggttaggt aaggataggt
     2281 tagagttagg taaggatagg ttagagttag agagagataa aaataaagca acccgcttta
     2341 ttacacccac catcgatgaa ataaaaacat ttctaaatga tcaagaatac gaaacagaca
     2401 ctgataggta tgctgttcgc ttctataatt tctatgaatc aaaagagtgg aaagtaggca
     2461 aagaaaagat gaaagattgg aaagctgcta taaggggatg gttaagcaga gataacatca
     2521 agaaaaagga agaggtcgca gtggaaacaa tcgatgattt gaatgatccc atctgggaag
     2581 aaataaaaaa attataaaag gagaaagaaa atgattacaa tttataccgt gccaagttgc
     2641 agttcgtgta gaaaagcaaa ggattggttt aatgaatggg aaataccgtt cgtcgaacac
     2701 gatattacaa aaacaccatt agaaaaggag gtgcttcttg acatccttta taaagcagag
     2761 aatggtgcag aagatattat ttcaacgcgc agtaagttgt tcaaagaaaa gaaaattgat
     2821 ttcaaaacaa tgacactatc a
//
LOCUS       DAOSEX010000094         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_33751_length_2836_cov_2.746494, whole genome shotgun sequence.
ACCESSION   DAOSEX010000094 DAOSEX010000000
VERSION     DAOSEX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33751_length_2836_cov_2.746494"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PK612_02375"
     CDS             <1..973
                     /locus_tag="PK612_02375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020754951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOH94990.1"
                     /translation="VPLAHKLYRTSLRRAGIKSPRHYDLNQSFSDGNPVPLGDSKYLV
                     KAATKMYDELSPETGEFFRFMVDKELLDLDARPGKRGGGYMTYFPRYQAPFIFANFNG
                     TAGDVDTLTHEVGHAYQGYESRHIKVSEYRQPTLEACEIHSMSMEFFAWPWMESMFDD
                     ADKYRLSHLEGTIKFLPYGVAVDEFQEGVYANPEMTHEERCAMWREIEKKYLPIRNYK
                     GAPFLEKGTFWLRQSHIFSSPFYYIDYTLAQVVAFQFATEMWKNRDRAWNKYVKLCRL
                     GGRYPFVELLAKAKLRNPFEDGNVRRVITPLKRKLKELTQVLESKND"
     gene            complement(1021..1689)
                     /locus_tag="PK612_02380"
     CDS             complement(1021..1689)
                     /locus_tag="PK612_02380"
                     /inference="COORDINATES: protein motif:HMM:NF003994.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOH94991.1"
                     /translation="MSKEKKQISKNLVYRVPVYLTVSRRLLKDGARYITAPQISAITG
                     INVETVKKDLSQICKTPGNPKLGREADVLVSEIIEFGVYTKLTNAVIIGAGHLGSALL
                     NYDGFNEWGLQIIAAIDNNPALINTTINDKQIHGVDDLDKIIKEYNVQIAIITVPKEY
                     AQELVDHAVAAGVKAIWNFAPTHISVPKDVVLQNENMASSLSLLNYNLNLKTSKQKRK
                     TKRK"
     gene            complement(1792..2715)
                     /locus_tag="PK612_02385"
     CDS             complement(1792..2715)
                     /locus_tag="PK612_02385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH94992.1"
                     /translation="MKKKISLIIPCYNEEEMIDILYDEINKVLVKLPKYDFIFLFVDD
                     GSKDQTLSLIKAKAKKDHRVKYISFSRNFGKESSMLAGLEAAQKLDVDAALLMDADMQ
                     DPPSLMLEFFKYYEEGYKYIFTRNKTRKGQALLKRFFANAFYVIYAWLTGDKNSVHSA
                     RDFALLDRDVINAFLTYKDKHRYSKGISSQIGFKKKCIEYDFQERQAGTTKWSFRKLF
                     KYAMTGIKQFSHVYILVPNLISIFLSIALILQIIFGHIYKYDYRYIYIAITAGVLLLN
                     ITLKYVMKLGYDIRDQLLNRPHYLTAETNIE"
BASE COUNT          912 a          491 c          512 g          921 t
ORIGIN      
        1 cgtcccactt gcgcataaat tatatcgcac aagtttacgc cgcgcaggta ttaagtcacc
       61 acgtcattat gacttaaacc aaagctttag cgatggtaac ccagtgccac taggtgacag
      121 taaatattta gtgaaagccg caactaaaat gtatgatgaa cttagtcctg aaaccggaga
      181 attcttccgg tttatggttg ataaagaact tttagattta gatgctcgtc ctggtaaaag
      241 aggtggtggt tacatgactt acttcccgcg ttaccaagca ccatttatct ttgctaattt
      301 taacggaaca gctggtgacg tcgacacttt aacgcatgaa gtaggacacg cttatcaggg
      361 gtacgaaagt cgtcatatta aagttagtga atatcgtcaa ccaacactcg aagcttgcga
      421 aattcatagc atgagcatgg aattttttgc ttggccatgg atggaaagta tgttcgatga
      481 cgctgataaa tatcgcttgt cacacttaga aggtacgatt aaattcttgc cctatggcgt
      541 tgccgttgat gaattccaag aaggcgtata cgctaatcca gagatgactc atgaagagcg
      601 ttgtgccatg tggcgggaaa ttgaaaagaa atatttacca attcgtaatt acaagggcgc
      661 tcctttccta gaaaaaggta cattctggct ccgtcaaagt catatcttta gtagtccgtt
      721 ttactatatt gactacactt tagcacaagt cgttgctttc caatttgcaa cagaaatgtg
      781 gaagaatcgt gatcgtgcat ggaataagta tgtgaaactt tgccgccttg gtggtcgtta
      841 tccatttgtg gaattactag cgaaagctaa acttcgtaat ccgtttgaag atgggaatgt
      901 acggcgtgtt ataactccac ttaaacgtaa attaaaagag ttaacacaag tgttagaaag
      961 caaaaacgat taaagcttaa attgtattaa aagggctgca atttgcagcc cttttcctta
     1021 ttatttccgt tttgttttcc ttttttgttt tgatgttttt aaatttaaat tgtaatttaa
     1081 aagactgaga ctacttgcca tattctcatt ttgtaagaca acatcttttg gtacacttat
     1141 atgtgtagga gcaaagttcc aaattgcttt aacacccgcc gcaacagcgt gatcgactaa
     1201 ctcttgagca tattcttttg gtacggtgat aatagcgatt tgtacgttgt actctttaat
     1261 gattttatct agatcatcaa ctccatgaat ttgtttatca ttaattgtag tgttaattaa
     1321 tgctggatta ttatcaattg cggcaataat ttgcagaccc cattcattaa agccatcata
     1381 attaagcaag gcactaccaa gatgaccagc accaataata acggcattag ttaattttgt
     1441 gtacacacca aattcaataa tttcagatac taaaacatca gcttcacggc caagttttgg
     1501 gttacctgga gttttacaaa tttgtgataa atcttttttg actgtttcaa cattaatccc
     1561 ggtaatcgca ctaatttgcg gtgcagttat gtagcgggca ccatctttta aaagacggcg
     1621 cgagaccgtt aaatatacag ggacacggta tacgaggttt ttggatattt gttttttctc
     1681 tttactcata aaaaaagaaa cctttctgca ttacgggaaa attttccgtc agccttatta
     1741 taaaggaaaa cattaaaaaa taacaacatt gatataagca tatcggtatt tttactcgat
     1801 atttgtttca gcagttaagt aatgtggccg atttaagagt tgatcacgaa tatcgtatcc
     1861 taacttcatg acatatttaa gcgtaatgtt cagcaataaa accccagcgg taattgctat
     1921 ataaatataa cgatagtcat acttataaat atggccgaat ataatttgta agattaaagc
     1981 aatgcttaag aagatgctga tgaggttagg cactaaaata taaacatgtg aaaactgttt
     2041 aatcccagtc atcgcatatt taaatagctt tctaaacgac catttagtag tgccagcttg
     2101 ccgttcttgg aaatcatatt caatgcattt ctttttaaaa ccaatttgtg aagagattcc
     2161 tttagaataa cgatgtttat ctttataagt caggaaagca tttataacat cacggtcaag
     2221 aagggcaaaa tcgcgggcac tatgcacact atttttgtcc cctgtaagcc aagcataaat
     2281 cacataaaat gcgttagcga agaagcgttt taaaagcgct tgtcctttgc gtgtcttatt
     2341 acgggtaaag atatatttat agccttcttc ataatattta aaaaattcaa gcataagaga
     2401 tggcggatct tgcatatcag cgtccataag taacgctgca tcaacatcaa gcttttgtgc
     2461 ggcttcaagg ccggcaagca ttgaagattc tttgccaaag tttcgcgaaa aagaaatata
     2521 tttaacgcga tgatctttct ttgcttttgc tttgattaat gacagtgttt gatcttttga
     2581 tccgtcatca acaaaaagaa agataaaatc atattttggt aattttacta aaactttatt
     2641 aatttcatca tagagaatat caatcatttc ttcctcgtta taacaaggaa ttatgaggct
     2701 tattttcttt ttcataaata actcccttaa ttaataaaat gatatcattt tatggatagt
     2761 aagtaaaaat ttattatttt cataatagaa gaaaaggtta aattgacaag ataaaaaact
     2821 gaaagaatca tttttt
//
LOCUS       DAOSEX010000095         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_33948_length_2824_cov_2.564103, whole genome shotgun sequence.
ACCESSION   DAOSEX010000095 DAOSEX010000000
VERSION     DAOSEX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33948_length_2824_cov_2.564103"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PK612_02390"
     CDS             complement(<1..712)
                     /locus_tag="PK612_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOH94993.1"
                     /translation="MKFSQYPFNVPDVPKIQKQLTKYIEQFKNAKDTNAASRVMRKIS
                     KYVDDFVTDAVIISVKFSQDSRNEEYVKAQEYVDHHFPYLSALMNEYNKLLVASPFRA
                     ELEKKWGSYLFKMIETQLKTFDPSIIELLQKESQLTNEYSKLIASAQIKFDGEVYNIP
                     QMGKFTQDKNRDVRKRASAAVVKFFEENNDELGRIYHELVQVRHAMAVKLGYKNFIEL
                     GYLKLGRLDYTAKEVAGYR"
     gene            complement(727..2328)
                     /gene="gpmI"
                     /locus_tag="PK612_02395"
     CDS             complement(727..2328)
                     /gene="gpmI"
                     /locus_tag="PK612_02395"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOH94994.1"
                     /translation="MNKRPIILTILDGYGYSEETKGNAVKKAKTPNINAFYKNNPTVL
                     IEASGEAVGLPAGQMGNSEVGHQNIGAGRIVYQSLTYINQLIKDGRFFENEVFVNAVK
                     HTLKYNSKLHLMGLVSDGGVHSHINHIKAFLDLAKMHGAKEVYLHALMDGRDVAPQAG
                     PEYIQELVDYMAKINFGKLATIGGRYYGMDRDKNLNRTNVHYDAIVNRKGLFFSDFRE
                     YFKEQYETLPKNGFDPSDEFLVPGINKNVNGQLGDNDAVIFFNFRPDRAIQFGTIVTN
                     PYFYQNPPLKDGKPEYKAFVPSPILKNIYYACTMKYADSVHGHIALSLPKLDNGLGVF
                     LADKGYTQLRIAETEKYAHVTFFFDGTVNYDGVEKPELKGARRILVPSPKVATYDLQP
                     EMSAPGVTDALLKELDKGDLDVVILNYANPDMVGHTAIESAVIKALETVDACVGRILE
                     WVEKHDATIMITADHGNSEKLLDEEGKPFTAHTSNPVRFGINRKDIKLMKEGGKLSNI
                     APTIIELLGEVPPSEMTEPSLIIKN"
     gene            complement(2400..>2824)
                     /locus_tag="PK612_02400"
     CDS             complement(2400..>2824)
                     /locus_tag="PK612_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94995.1"
                     /translation="LAFVFMHISLDYTRQLKKSKETIHSLFAGQIALFAMIGKELESP
                     NSEAQVMNELLEKREFTELNKLAAEKERAYQELAAKKKDTTPQTAQLLQGLSENVVLI
                     RNEISRHNKLVDNINVNVDSVIFSLFVVILRLKRLTRI"
BASE COUNT          832 a          529 c          485 g          978 t
ORIGIN      
        1 cacgatagcc cgcgacttct ttagcggtat agtcaagccg gcctaatttt aagtaaccaa
       61 gttcaataaa gtttttataa ccaagtttaa ctgccattgc atggcgaact tgcacaagtt
      121 cgtgataaat gcgaccaagt tcatcattgt tttcttcaaa gaatttaaca acagcggcac
      181 tagcgcgttt tcttacatca cgattcttat cttgagtaaa tttacccatc tgcgggatat
      241 tgtatacttc accatcaaac ttaatttgtg cactggcaat aagctttgaa tattcattcg
      301 tgagttgtga ttctttttgt aataattcaa tgattgaagg atcaaatgtt ttaagttgtg
      361 tttcaatcat tttaaataag taacttcccc acttcttttc aagttcagcg cggaagggag
      421 aagcaacgag cagtttattg tactcattca ttaatgcact taaatacggg aaatgatgat
      481 cgacatattc ttgggctttc acatattctt cattgcgtga atcttgcgag aatttaacac
      541 taataataac agcatcagtg acaaagtcat caacatactt agaaatcttg cgcataacac
      601 gagatgccgc atttgtgtct ttagcatttt taaattgttc aatatacttt gttaattgct
      661 tttgaatttt tggtacgtca gggacgttaa atggatattg actaaatttc atatttgttt
      721 tcctttctaa tttttaatta ttaaacttgg ttcagtcatt tcactaggcg ggacttcccc
      781 aagtaattca atgattgttg gggcaatatt actaagtttg ccaccttctt tcataagttt
      841 gatatcttta cgattaatcc caaagcgtac tggattactc gtatgagcgg taaatggttt
      901 accttcttcg tcaagtaatt tttccgagtt accgtggtca gctgtaatca taatcgttgc
      961 atcgtgtttt tccacccatt caaggatgcg cccaacacat gcgtccacag tttcaagtgc
     1021 tttaatcact gcactttcaa ttgcagtatg accgaccata tcagggtttg cataatttaa
     1081 tataacaaca tcaagatcac ctttatctaa ttctttaagt aaagcatccg tgactcctgg
     1141 cgctgacatt tctggttgta agtcataagt cgctactttg ggggacggca ctaggatacg
     1201 acgcgcacct ttaagttcag gcttttcaac accgtcataa ttaaccgtac catcgaagaa
     1261 aaatgtaaca tgcgcatatt tttctgtttc agcgattcgt agttgtgtgt aacctttatc
     1321 cgctaaaaag acacctaagc cattatcaag tttaggtagt gataaagcga tatgtccatg
     1381 aactgaatcg gcatatttca tcgtacatgc gtaataaata ttttttaaga ttgggcttgg
     1441 cacaaatgct ttatattctg gttttccatc ttttagtggt gggttttgat agaaatatgg
     1501 attagtgaca atcgttccaa attgaatcgc acgatctgga cggaaattaa agaaaataac
     1561 agcgtcatta tctcctaatt ggccattgac atttttatta attccgggaa ctaaaaattc
     1621 atcacttgga tcaaagccgt ttttgggtaa tgtttcgtat tgttctttga aatattctct
     1681 aaaatcacta aagaataagc ccttacgatt cacaatcgca tcgtaatgga cattcgtacg
     1741 atttaaattc ttatcacgat ccattccgta ataacggcca ccgatggtag cgagtttacc
     1801 aaaattaatt ttcgccatat aatccactaa ttcttggata tattctgggc cagcttgggg
     1861 cgctacatcg cgtccatcca ttaaagcgtg taaataaacc tctttagcgc catgcatttt
     1921 tgctaaatct aaaaaggctt taatgtgatt aatatgtgaa tgaacaccgc catcactaac
     1981 taaacccatt aaatgcaatt ttgaattgta tttaagtgta tgtttaacgg cattaacaaa
     2041 tacttcattt tcaaaaaagc gaccatcttt aattagttga ttaatataag ttagtgattg
     2101 ataaacaatg cggcctgcac caatattttg gtgaccgact tcactatttc ccatctgccc
     2161 agcgggtaaa cctaccgctt cgcctgaggc ttcaattagg acggtcggat tatttttata
     2221 gaatgcgtta atgtttgggg tttttgcttt tttaacggca ttcccttttg tttcttcgct
     2281 atagccgtag ccatctaaga ttgtaagtat aataggacgt ttattcataa taaaaaacct
     2341 ctttccgata gtaattatat tactataaag ggtaattgta agcaaaaatg cttatttaac
     2401 tagattctag taagtctttt taatcgtaaa ataacgacaa acaatgaaaa tataacagaa
     2461 tcaacattaa cattaatgtt atccactagt ttgttatgac gagatatttc attacgaata
     2521 agtacaacat tctcactgag accttgtagt agttgcgctg tttgtggagt tgtatctttc
     2581 tttttcgctg ctaactcttg ataagcgcgc tctttttccg ccgctagctt attaagttcg
     2641 gtaaattcac gtttttctaa aagttcattc attacttgtg cttcactatt tggtgactct
     2701 aattctttac caatcattgc aaaaagggca atttgacctg caaaaaggct gtgaattgtt
     2761 tctttgctct tttttaattg tcgtgtgtag tcgagtgaaa tatgcataaa aacaaatgct
     2821 aaga
//
LOCUS       DAOSEX010000096         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_34272_length_2806_cov_3.177027, whole genome shotgun sequence.
ACCESSION   DAOSEX010000096 DAOSEX010000000
VERSION     DAOSEX010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34272_length_2806_cov_3.177027"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..827
                     /locus_tag="PK612_02405"
     CDS             138..827
                     /locus_tag="PK612_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94996.1"
                     /translation="MIIIYGGAFFLVIVIAIFSALVLGGIKITIINALIALVVAAFLT
                     YRVTNYRKDIEKRRFMFNFMEYFILNFDIQKTVEATLTTIYPLLDVKKSRVYLTMNED
                     GMLLLEKLRLTFAHQYYESFLEMVKLINEHGGEMLKVAEVLLFSISNSETQLIKLTRI
                     DNAYLIKFIFNWFFIMLVAIVFRLALDGFLKFETLPLIYVAGMEVFIAIFLTSIVLVF
                     ENRIRRTRRVS"
     gene            817..1422
                     /locus_tag="PK612_02410"
     CDS             817..1422
                     /locus_tag="PK612_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94997.1"
                     /translation="MLVKWLERSAGLLLIIVGAILYFWKKNLTLALIFAGVGFTIVVI
                     TFIPQKQKSNITLADFINVFTHFRVALDNNLNVYRALNITLTTTSGAMSALLESLINA
                     LENDHSVTPFINFSLPFKHPLVSQIMINVYMLVNHGVDPKRLWQFNYLFETLVKEHND
                     EEIALHESSYERFNISLFIGTGIMIMTLMISIMGMIGEYIV"
     gene            1419..1757
                     /locus_tag="PK612_02415"
     CDS             1419..1757
                     /locus_tag="PK612_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94998.1"
                     /translation="MSNIMSLVYSFILIFPTILLGVDFFIIGKIIVELETRATIVSYI
                     ISEEGGIRPSLVNELAEEGITITCEGECSYISVGETIKYQLIKTYTPIIIKNEDINIY
                     VTRTTIVGYL"
     gene            1774..1911
                     /locus_tag="PK612_02420"
     CDS             1774..1911
                     /locus_tag="PK612_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH94999.1"
                     /translation="MSELKGQILGLLLVVVIFATIAAAFKASVNNQLSEIASHESALV
                     I"
     gene            2013..2237
                     /locus_tag="PK612_02425"
     CDS             2013..2237
                     /locus_tag="PK612_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH95000.1"
                     /translation="MTNLERIEQIFTELLKVEKIEPEMELKALGLDSLDLVEVMMRLE
                     EEFGIEFSNDEMLGFSTVADVVAEIERKTK"
     gene            2304..2378
                     /locus_tag="PK612_02430"
     tRNA            2304..2378
                     /locus_tag="PK612_02430"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2336..2338,aa:Asn,seq:gtt)
     gene            2382..2458
                     /locus_tag="PK612_02435"
     tRNA            2382..2458
                     /locus_tag="PK612_02435"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2416..2418,aa:Glu,seq:ttc)
     gene            2550..2626
                     /locus_tag="PK612_02440"
     tRNA            2550..2626
                     /locus_tag="PK612_02440"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2584..2586,aa:Glu,seq:ctc)
BASE COUNT          896 a          413 c          530 g          967 t
ORIGIN      
        1 ttacaagctt ttccgtttgt tattcattta gcaaaaaaat ataaggcaga tggctcgatt
       61 tatcgttatg tcgaaaaaat cactgaatac aatgttgaca ctgatgaaat tgaaatactt
      121 tatcacgagg tgattccgtg attattattt atggtggtgc tttctttttg gttatagtaa
      181 tagcaatatt tagtgcccta gtgctaggcg gtataaaaat aacgattatc aatgcactaa
      241 ttgcactagt tgtcgctgct tttcttacat atcgcgttac taattatcgc aaagacattg
      301 aaaaaagacg ctttatgttt aattttatgg aatattttat tcttaacttt gacattcaaa
      361 aaacagtaga agcaacacta actacaatat atccactgct tgacgtaaaa aaatcaagag
      421 tatatttaac aatgaatgaa gacgggatgc ttttacttga gaaactgcga cttacttttg
      481 ctcatcaata ttatgaatca tttcttgaga tggtgaaact tattaatgaa cacggtggtg
      541 aaatgcttaa agtagcggaa gtactgcttt ttagtatttc taatagtgaa acgcaactta
      601 ttaaattaac tagaattgat aatgcttatc ttattaaatt tatttttaat tggtttttta
      661 ttatgttagt agcaatcgtc ttccgcctag cgcttgatgg ctttttaaaa tttgaaacat
      721 taccactaat ttatgttgcg ggtatggaag tctttatcgc tatattttta acaagcattg
      781 ttttagtatt tgaaaataga atcaggagaa cacgccgtgt tagttaaatg gttagaacgt
      841 agtgccgggt tattattaat catcgttggc gctattttat atttctggaa gaaaaactta
      901 acacttgcac tgatatttgc tggtgttggt tttacaattg ttgttattac ctttatcccc
      961 cagaagcaaa agagtaatat cactttagcg gattttatta atgtattcac ccattttcgt
     1021 gtagcgcttg ataataactt aaatgtatat cgcgctttaa atattacatt aacgacgaca
     1081 agtggtgcga tgtccgctct tttagaatca ttgattaatg ctttagaaaa tgatcatagt
     1141 gttacccctt ttattaattt ttccttaccc tttaagcatc cgcttgtttc gcaaattatg
     1201 attaatgttt atatgcttgt taatcacggt gttgatccaa aaaggttgtg gcaatttaat
     1261 tatttatttg aaacactagt gaaagaacat aatgatgaag aaatagcgct acatgaaagt
     1321 agttatgagc gctttaacat tagtttattt attggtacag gaattatgat tatgacactg
     1381 atgataagta ttatggggat gattggagaa tatatagtat gagtaatatt atgagccttg
     1441 tttattcatt catcctaatt ttcccgacaa ttttgcttgg tgttgacttt ttcatcattg
     1501 ggaaaatcat tgttgaacta gaaacaaggg caacaattgt tagctatatt attagtgaag
     1561 aagggggcat taggccaagt ctagttaacg aactagcgga agaaggcata acgattactt
     1621 gtgaaggcga atgctcttac atcagtgtag gggaaacaat taaatatcaa ttaattaaaa
     1681 catacactcc gattattatt aaaaatgaag acattaacat ttatgtgaca agaacaacaa
     1741 ttgttggtta tttataatta aggaggtaat tttatgtcag aactaaaagg acaaatttta
     1801 gggttattac ttgttgttgt tatttttgcc acaattgctg cggcctttaa agcatcagtt
     1861 aataatcaac tttcagaaat tgctagtcac gaaagcgcgt tagtaattta attaactttt
     1921 attcgtcgct tagcagtgaa gaaaccttgg aatcgttttt atttgaaata atgcgtgcta
     1981 tttgttaaaa tttgaattct aaggagaata ctatgacaaa tttagaacgt attgaacaaa
     2041 tattcaccga attgctcaag gttgaaaaaa ttgaacctga aatggaatta aaagcattag
     2101 gacttgacag ccttgattta gtggaagtca tgatgagact cgaagaagag tttggtattg
     2161 aatttagtaa cgacgaaatg cttggatttt ccacagtcgc tgatgttgtc gctgaaattg
     2221 agcgtaaaac taaataataa taataattat tattgccaag acttaggaag tgtgatattc
     2281 ttatgactgc ctaggtctaa catgcctagt tagctcagcg gcagagcaat cggctgttaa
     2341 ccgatcggtc gtaggttcaa atcctacact aggcgccagc tggcccgttg gagaagcggt
     2401 ttaactcaca tgcctttcac gcatgcattc atgggttcga atcccgtacg ggtcgccata
     2461 ggtaaatcaa acggtactat aaagtgccgt ttttttgttt tcattgcaca cacttatttt
     2521 gtaagtgcta aaataataat cgcgattatg gcccgttgga gaagcggttt aactctcatg
     2581 cctctcacgc atgcattcat gggttcgaat cccgtacggg tcaccaaact ttcgtgtaac
     2641 ggcacagttt gcgtgccgtt tttttatatt aaatttgggt tttaaagttt atttggctta
     2701 caattagttt aaagattaat gaaaagaggt aaaagttatg gtagatacaa atgagattag
     2761 aagaattttt aacgagctag aaagacaagc aaagacttta ttaaat
//
LOCUS       DAOSEX010000097         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_34492_length_2793_cov_3.288167, whole genome shotgun sequence.
ACCESSION   DAOSEX010000097 DAOSEX010000000
VERSION     DAOSEX010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34492_length_2793_cov_3.288167"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PK612_02445"
     CDS             <1..662
                     /locus_tag="PK612_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003248045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95001.1"
                     /translation="TKSQFSFNTKGGRCEHCEGAGVTRIAMNFLPDVYVKCEMCDGQR
                     YHADTLSIHYRGKNIVDVLNMTVEEALEFFANRPQIVRRLQTLYDIGLGYIKLGQPAP
                     ELSGGEAQRLKLAFELQKRPTGKTLYLLDEPSTGLHADDVGRLITSLQAIVANGDTVI
                     VIEHNLDIIKVADYIIDLGPGGGNYGGKVVASGTPEEIVKVKGSYTGEYLAPYLKKEK
                     K"
     gene            664..1974
                     /gene="lgt"
                     /locus_tag="PK612_02450"
     CDS             664..1974
                     /gene="lgt"
                     /locus_tag="PK612_02450"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOH95002.1"
                     /translation="MDLTLVVVVIFGLIFIGAVTVLGLSLNEFVKKEEDINTLFKGKH
                     RLIAISVLSGAVSVLMLFLPLMVLSNSVLHSLLIGLGSFLFALMLLTFIAAFVLHYYK
                     FNVLQREWIKESKIVTIISGVLSIVFLFVLLEGLTLAEIIKFPLPRGIPFGDSPVIAF
                     YAIFILTGALLVLAITDHEFYKKYGRHGILENVFYVAFPAGIIGARIWYVIGEWNNPE
                     SGFAENPLTIFAIRDGGLAIMGGALFGIIAGVWFFVKRRKAYDIGFAADIIIPTILVA
                     QAIGRWGNFFNQEVYGGVITDISKWWFLPEFIKRQMFILGKYRQPFFLIESALNLTGY
                     FVIRYGVGEGLKKYRKPFDMAFMYIVWYGLVRFIMEPLRDPMFRMGAGGKWSEYNALI
                     FFVVGVALIVLNHIFDFHKLLTRKKGTAEVVSNEPSESVEKNEE"
     gene            1964..2611
                     /locus_tag="PK612_02455"
     CDS             1964..2611
                     /locus_tag="PK612_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH95003.1"
                     /translation="MKSKYDLVVFDLDGTIADSDLGLVKIGIKMSETYLPRKDINMDD
                     YLFLNGPTLDETMPYLFPGYPLEELIAKYNEYLPDSVKDITLFKGAKELIKTLKNNNI
                     ELALLTNRARASTEQILKRHGVHSYFKTLVCVDDGFKKKPSGEGLVHIMKTLKSAPDK
                     TLFVGDNWRDILAGQDAGVDVAFIHQHRRPHRLDVSATYVLDDISDVLGVVQSDE"
     gene            2604..>2793
                     /locus_tag="PK612_02460"
     CDS             2604..>2793
                     /locus_tag="PK612_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011178815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH95004.1"
                     /translation="MNKEIIPILIIGAGPAGMTAAIYLSRANATFKIVDKYAPGGKMN
                     ITQDVENYPGIETISGPDL"
BASE COUNT          823 a          436 c          626 g          908 t
ORIGIN      
        1 acacgaagag tcaattttcg tttaacacta aaggtggtag atgtgaacac tgtgaagggg
       61 cgggtgttac acgcattgct atgaactttt tacctgatgt ctatgtaaaa tgtgaaatgt
      121 gtgacggaca aagatatcac gctgacacgc tttcgattca ttatcgcggc aaaaacattg
      181 ttgatgtact taatatgaca gtcgaagaag cgcttgaatt ctttgcaaat aggccgcaga
      241 ttgtacgccg tttacaaact ttatacgata ttggtttagg ttatattaag cttggacaac
      301 ctgcaccaga acttagtggc ggggaagcac aacgcttaaa acttgccttt gaattacaaa
      361 agcgtccgac tggtaaaaca ctatatttac ttgatgaacc gtcaaccggc ttacatgcag
      421 atgatgttgg gcggcttatt acttcactcc aagctattgt cgctaatggg gatactgtta
      481 ttgttattga gcataatctt gatattatta aggttgctga ttatataatt gatttaggtc
      541 caggcggtgg gaattatggt ggaaaagttg tcgctagtgg gaccccagaa gaaatagtta
      601 aagttaaagg gtcgtatact ggagaatatt tagcgcccta cttaaaaaag gagaaaaagt
      661 aatatggatt taacactcgt ggtagttgta atatttggct tgatctttat tggagcagtg
      721 acggtccttg gtttaagtct taatgaattt gtaaaaaaag aagaggatat taataccttg
      781 ttcaaaggta aacaccgttt aatcgccatt agtgtcttaa gcggtgcagt gtcagtgtta
      841 atgctgtttt taccactgat ggtgctatca aatagtgtgt tacattcttt gttaattggt
      901 ttaggtagtt ttctctttgc tttaatgtta ctcactttta ttgctgcttt tgtcttacat
      961 tattataaat ttaatgtgtt acaaagagag tggataaaag aaagtaaaat agtaacgatt
     1021 ataagcggtg ttttaagtat tgttttcctt tttgtattgc ttgaaggctt aacacttgcg
     1081 gaaattataa agttcccatt accacgcggt atcccttttg gtgatagtcc cgttatcgct
     1141 ttttacgcaa tattcattct aactggtgcg cttttagtat tagcgattac tgatcacgaa
     1201 ttttataaaa aatacggtag acacggtatt ttagaaaatg ttttctatgt tgcttttcca
     1261 gcgggaatta tcggcgcgcg catatggtat gtgattggtg aatggaataa tcctgaatca
     1321 ggttttgcgg aaaatccgtt aacgattttt gcgattcgag atggtggtct agcgattatg
     1381 ggcggagcgc tttttgggat tattgctggg gtgtggttct ttgtgaaaag acgtaaagct
     1441 tatgatattg gttttgccgc agacatcatt attccgacga tcttagttgc tcaagcaatt
     1501 ggccgctggg gtaatttctt taatcaagaa gtctatggtg gggttattac tgatatttca
     1561 aaatggtggt ttctccctga atttattaag cgccaaatgt ttattttagg gaaataccgt
     1621 caacccttct tccttattga atcagcgctt aatttaacgg gttactttgt gattcgttac
     1681 ggtgttggtg aaggacttaa gaaatatcgt aaaccatttg atatggcatt tatgtatatt
     1741 gtgtggtatg gtttagtgcg gtttattatg gaaccgttac gtgacccaat gttccgcatg
     1801 ggagcaggtg gtaagtggtc agagtataat gcccttatct tctttgtcgt gggtgttgcc
     1861 ctcattgttt taaatcatat ctttgatttc cacaaacttt taacaagaaa gaaaggaaca
     1921 gctgaagtag taagtaatga accttcagaa agtgtagaaa aaaatgaaga gtaaatacga
     1981 tttagtggtg tttgatcttg acggcacgat tgccgatagt gatttaggac tcgttaaaat
     2041 cgggattaaa atgagtgaaa cttatttacc gcggaaagac attaatatgg atgactatct
     2101 ctttttaaac gggccaacgc ttgacgaaac aatgccgtac ttattcccag gttacccctt
     2161 agaagagtta atagctaaat ataatgaata cttaccagat agcgttaaag atattacgct
     2221 ttttaaagga gcgaaagaat taattaaaac tttaaaaaat aataatattg agttagcttt
     2281 actcactaat agagctagag caagcactga acaaatttta aaaagacacg gtgttcattc
     2341 ctactttaaa acgctcgtat gtgttgatga cggttttaaa aagaaaccaa gtggtgaagg
     2401 cttagtgcat attatgaaaa cgctaaaaag tgcacctgat aaaacacttt ttgtcggtga
     2461 taattggcgt gatatcttag ccggtcaaga cgcaggagtc gatgttgcgt ttatccatca
     2521 acatcgccgt ccgcatcgcc ttgatgtaag tgcaacatat gtattagatg acattagtga
     2581 tgtcttagga gtagtgcaaa gtgatgaata aagaaattat tccgatttta attattggtg
     2641 cgggtccggc ggggatgacg gcggcaattt atctttcgcg cgcaaatgcg acatttaaga
     2701 ttgttgataa atatgcgcca ggcggcaaaa tgaatattac gcaggacgtc gaaaattatc
     2761 caggtattga aaccatcagc ggacctgacc ttg
//
LOCUS       DAOSEX010000098         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_34857_length_2773_cov_2.530537, whole genome shotgun sequence.
ACCESSION   DAOSEX010000098 DAOSEX010000000
VERSION     DAOSEX010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34857_length_2773_cov_2.530537"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PK612_02465"
     CDS             <1..1066
                     /locus_tag="PK612_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95005.1"
                     /translation="EFFSGATYTFEELSKFRYFNLENYAGNEIRIKQISLTYTDVPPA
                     AWVNLTSVTKAKDHYDIKRGVHLLIDKPTIEPSNAFYDYFLTTEDTRLRINGPYVVAE
                     DLCENAVVTINTFGRDAGGERLTTTFTVSVTEAPLTIDGAVGMGYGSIDEKLYRFENV
                     RVQDATLDSDNIVLHASATATNKINVMHPGFNPLNTYTYVKGGTMSLFAKVRRNTTTG
                     KNFLVEPEVISYTDKAILFANTIMASDEVGQCVTKYATVRAIINEMTVEELAKLKNAG
                     VVNGINFSPVRARYVAWALHLGESDPYGDGTPISGMPLVPENSTTQNNNVLLVILLSF
                     SVAVFLYFLTSKRKKLYLKK"
     gene            complement(1141..1884)
                     /locus_tag="PK612_02470"
     CDS             complement(1141..1884)
                     /locus_tag="PK612_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOH95006.1"
                     /translation="MAEKNLALLIDAENISPDYMSIIRDEANKYGNLTYKRIYGDWAS
                     SNLTKWRSKCIEYNLEQIQQVSYVSGKSTSDFTLVIDAMDILYSDQVDGFVLVSSDSD
                     FTRLVSRLRQNNKFIIGMGESKTPLSLVNSVDLFIYLDKIKNIRDKNVKPKKRGRPAA
                     DKKEEESIISLDDLIDVLTNIISETSDENGWAYWSNTNNTLVRKYPGFDPRNYGFKGK
                     ALQFFLANGFEKRNEGLDVFIRPKANNEQ"
     gene            complement(1900..>2773)
                     /locus_tag="PK612_02475"
     CDS             complement(1900..>2773)
                     /locus_tag="PK612_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95007.1"
                     /translation="THHEFGSGDKICYHGVLDIYREPKPAAYVYTSNLTSDPVLFPLF
                     RGDNGDLPESKLYKTMVLSNASYIDLYKDDEVVKRYYPRRDLYKHLPHPPFIIDTFIS
                     DNFKSTIKFSKRDRKKIAKLLNYAALNNFSRLSLWQKLQLGWLIVKYRLKWDNLVELW
                     FLNVVGWGDKSSGYTLVGYNDEDIEIIRKRIENTTTTTYEIELLKNELVNTDTFDTTI
                     VTIRSLSPYRNHYDFSPVSVVVTGPLKVYGPEIQNLHAGVLNVYVRSINTTGRGTITI
                     GVGGTIKTFNVEIK"
BASE COUNT          849 a          514 c          526 g          884 t
ORIGIN      
        1 tgaatttttt tcaggtgcca catatacatt tgaagagcta agtaaattta gatattttaa
       61 ccttgaaaat tatgcgggta atgaaatccg gattaaacaa atttcattaa cttacactga
      121 tgttccaccg gctgcttggg taaatcttac tagtgttact aaagctaagg atcattatga
      181 tattaagcgt ggagtgcacc ttttaattga taaacccact attgaaccta gtaatgcgtt
      241 ctacgattat tttttaacaa cggaagatac acgtttacgg ataaatggcc cgtatgttgt
      301 cgccgaagac ctttgtgaaa atgcggtcgt gacaattaat acttttgggc gcgatgcagg
      361 tggtgagcgc ctaacaacaa cattcactgt tagtgtaact gaagcaccac tgactattga
      421 tggtgcggtt ggcatgggtt acggaagtat tgatgaaaag ctctatcgct ttgaaaatgt
      481 tcgcgtccaa gatgctactc ttgatagcga taatatcgtt ttacacgcta gtgctaccgc
      541 aacgaataaa ataaatgtca tgcaccctgg ctttaaccca cttaacactt atacctatgt
      601 aaaaggtggg acgatgagtc tctttgcgaa agtgcggaga aatacaacga caggtaaaaa
      661 cttcttagtg gaaccagaag ttattagtta cacagacaag gctattttgt ttgcaaatac
      721 aataatggca agtgatgaag tcggtcaatg tgtaacaaaa tacgccacag tgcgggcaat
      781 tattaatgaa atgacagtcg aagaactggc aaaattaaaa aatgcaggtg tagttaacgg
      841 aattaatttt agtcctgtta gagcgcgtta cgtcgcatgg gcattacatt taggtgaaag
      901 cgatccttat ggcgacggca ccccgatttc aggaatgcct ttagtgccag aaaattcaac
      961 tacacaaaat aacaatgtcc ttttagttat tttactttct ttcagtgttg cggtgtttct
     1021 atatttctta acatcaaaac gaaagaaact ttatcttaaa aaataggaat tatttgtagt
     1081 ttactaacaa aaaaaactcg gtgattacgt atcactgagc ttttctttta caaataataa
     1141 ttactgttcg ttatttgctt tagggcgaat aaagacatct aagccttcat tgcgtttttc
     1201 aaagccgttt gctaagaaaa attgtaaagc tttaccttta aagccgtagt tacgagggtc
     1261 aaaaccgggg tacttacgaa caagcgtatt gtttgtattg ctccagtatg cccaaccatt
     1321 ttcgtcactt gtttcactga taatgttcgt taaaacatcg attaagtcat caagactaat
     1381 gatgctttct tcctctttct tatcagcagc gggacggcca cgttttttcg gtttaacgtt
     1441 tttgtcgcga atgtttttaa ttttatcgag atatataaat aagtcgacgg aatttacaag
     1501 tgataagggg gtcttgcttt cacccatccc aataataaac ttattgtttt ggcgtaagcg
     1561 actaacaagt cttgtaaagt cactatcact actgacaagg acaaagccat caacttggtc
     1621 actatataaa atgtccattg catcaattac gagtgtaaaa tcactagtgc ttttaccact
     1681 gacgtaagag acttgttgaa tttgctctaa gttatattca atacatttac tgcgccattt
     1741 ggtcagattg ctacttgccc aatcgccata aatgcgctta taagttagat tgccatattt
     1801 attagcttcg tcacgaataa ttgacatata atcaggtgat atattctcgg catcaattaa
     1861 aagtgcgaga tttttttcgg ccattactgc tcctcctttc tatttaattt cgacgttgaa
     1921 ggttttaatc gtaccgccaa caccaatagt tattgtacca cgccccgtcg tgttaatgga
     1981 gcggacatac acattaagta caccagcgtg taagttttgg atttctggcc cgtaaacctt
     2041 taggggacca gtaacgacaa ctgaaacagg actaaagtca taatgattac gataaggact
     2101 tagactacgg attgtaacaa ttgttgtatc aaatgtatct gtgttcacta attcattttt
     2161 taaaagttcg atttcataag tcgtagtagt ggtgttttcg attctttttc taattatttc
     2221 gatatcttca tcgttatagc ccactagtgt gtagccgctt gacttatcac cccagccgac
     2281 aacattaagg aaccataatt cgacaagatt atcccattta aggcgatact taacaattaa
     2341 ccacccgagt tgtaactttt gccagagtga gagccgacta aaattattga gagcggcata
     2401 atttaatagt tttgcgatct ttttacgatc acgctttgaa aatttaattg ttgacttaaa
     2461 gttatcacta ataaaagtat cgatgataaa tggtggatgc ggtaaatgtt tatataagtc
     2521 acgccgcggg tagtagcgct ttacaacttc atcgtctttg tataaatcaa tgtaacttgc
     2581 gttacttagc accatcgttt tatataattt actttctggt aagtcaccgt tatcaccgcg
     2641 aaaaagcggg aataaaacag ggtcacttgt aaggttagaa gtatacacat acgccgcggg
     2701 tttaggttca cgatatatat ctaacacacc atgataacaa attttgtcac cactaccaaa
     2761 ttcgtggtgc gtg
//
LOCUS       DAOSEX010000099         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_34905_length_2770_cov_2.321547, whole genome shotgun sequence.
ACCESSION   DAOSEX010000099 DAOSEX010000000
VERSION     DAOSEX010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34905_length_2770_cov_2.321547"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="PK612_02480"
     CDS             <1..1766
                     /locus_tag="PK612_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95008.1"
                     /translation="WTEGPYILNRNGISYLTFTGTHVTSAGYRVAYSYSEEGPVKHTS
                     YVQDKKSIILNTDPEFNGLGHSATVLGPDLDSYYIVYHNLLNSGGPIRAYSMNRLYFN
                     GTDMVSPHARRYQNVAPEMATFTSHNAATDLEVDGDFLLSPTATKDVFTAEWNIKSSV
                     GQMVFSYEDANNYSYMTFDGRNVALKKVDGGTESDITSFALKTNHPTDVLHTFRIIYR
                     DGVLDVTYDNMNKVLGHKVTLAPGKVGYKGFDTAAIGFTGYSDFARGTSDNYYHKQEM
                     ALANSYHLETSELTTSVLQKVEGDYVSAHAKAGSYDLNLVTGDKARYLMYFDASEYYA
                     LSMTVPIEMMGKSVTVSIDGITKALKVPTYELYPEERYVKVELGRFPIYAGSHYVSVI
                     ATEEFRFNNFDFTVVTDSKATYENDLSTLGGDTLLYQNIWKIKRGGHYASSGNRQLLY
                     IGKGIISDVTIEADINFDGPTAANTVGFLLRAGNEAFSTWENNDSIQGYYVAMNNAQV
                     SIRRKDYNWSVFLDSYGGSFASETDIRLKASIKGNLIIVYVNGEEVIRVADAGAFRSG
                     KLGLYTDGASATYKNLKVTLG"
     gene            1831..>2770
                     /locus_tag="PK612_02485"
     CDS             1831..>2770
                     /locus_tag="PK612_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOH95009.1"
                     /translation="MMKKWLKFIAFSLLLTSCRLTPSSSLSEPSISIDTSVNFDPTID
                     MSTLETRYNTFNNSFDDAQIPNQWPNYGIGDPFVYRFNGFYYLYPSTSQRGVRAYRSI
                     DLMNWEPVTGEGLEYGYVLSPDYDESVVAYAPEVTYYDGWFYLCESQAGTGHYFFRSR
                     SPEGPFVPYTGNVGESIDGSFFIDDDGKAYFLRAKAETIRIRRFNDDFTLSNDAFDID
                     NTSMGGWTEGPYVLKRNGIYYLTFTGVHVGSPGYKVGYSYLQDSDTVFNRYGYTRAEN
                     ILMNTNEEWHTLGHSSTTMGPDMDSHYIAYHSRTSGV"
BASE COUNT          799 a          510 c          583 g          878 t
ORIGIN      
        1 actggactga aggtccctat atccttaatc gcaatggtat tagttactta accttcactg
       61 gtacgcatgt aacaagtgca gggtatcgcg ttgcttatag ttattcagaa gaaggaccag
      121 ttaaacacac gtcttatgtc caagataaga aaagtattat tttaaatact gatcctgaat
      181 ttaatggtct tggtcatagc gcaactgtgc ttggtcccga cttagattct tattacatcg
      241 tttatcataa tttacttaat tctggtggcc caattcgtgc ttactccatg aaccgtctct
      301 attttaacgg gacagatatg gttagcccac atgcgcgccg ctatcaaaat gttgcacctg
      361 aaatggcaac ttttacaagc cacaatgctg caacagactt agaagttgat ggtgatttct
      421 tgctttcacc aacggcgact aaagacgtct tcactgctga atggaatatt aaaagcagcg
      481 tggggcaaat ggtgttttct tatgaagacg ctaataacta tagttatatg acttttgacg
      541 ggcgtaatgt cgcccttaag aaggtcgatg gtggaaccga aagtgatatt acaagctttg
      601 cattaaagac taatcatccg accgatgtcc tccatacctt ccgtatcatt tatcgtgatg
      661 gtgtgcttga tgttacttat gacaatatga ataaagttct tggccataaa gtaactttag
      721 cgccaggaaa agttggttat aaggggtttg atactgctgc aattggtttt actggttata
      781 gtgactttgc tcgtggcaca agtgacaatt attaccataa gcaagagatg gcgctcgcta
      841 atagttatca tttagaaact agcgaactta caacttctgt gctccaaaaa gttgaaggtg
      901 attatgtaag tgctcacgct aaagcagggt cttatgatct taacttagta actggtgaca
      961 aggcgcgata cttaatgtac tttgacgcta gtgaatatta cgcgctttca atgactgtgc
     1021 ccattgaaat gatgggcaag agtgtgaccg ttagtattga cggcattact aaagcgctta
     1081 aagtcccaac ttatgaatta tatccagaag agcgctatgt taaagtggaa cttggacgct
     1141 tcccgattta tgcaggaagt cattatgtta gtgttattgc aactgaagag ttccgcttca
     1201 ataacttcga tttcacagtt gtaactgatt caaaagcaac atatgaaaac gacttatcaa
     1261 ccttaggcgg tgatacactt ctctatcaaa atatttggaa gattaagcgt ggtggtcatt
     1321 acgcaagcag tggtaaccgg caacttctct atattgggaa aggtattatt agtgatgtta
     1381 caattgaggc cgatattaat tttgacggtc ctaccgccgc taatacagtc ggtttcttac
     1441 tccgggctgg caatgaagct ttctctacat gggaaaataa tgattccatc caaggatatt
     1501 atgtcgccat gaataatgcg caagtatcaa ttaggcgtaa agactacaac tggtcagtct
     1561 tccttgattc ttatggcggc tcatttgcga gtgaaacaga cattcgctta aaagcttcaa
     1621 tcaagggtaa tttaattatc gtttatgtta atggcgaaga agtcattagg gtcgctgatg
     1681 ctggcgcttt tagaagcgga aagctcggac tttacactga cggagctagc gccacataca
     1741 aaaacttaaa agttacactc ggataactct cctcgttatc cttagctccc ttactccccg
     1801 taggggagct tattaatagg aggatattta atgatgaaaa aatggttaaa atttattgct
     1861 ttttctttac ttctcactag ttgtcgctta actccgtctt cttctttaag tgagccatca
     1921 atttcaattg atacaagtgt taattttgat ccaacgattg atatgagcac tttagaaaca
     1981 agatacaata cttttaataa ttcgtttgat gatgcccaaa ttcctaacca atggccaaat
     2041 tatgggattg gtgatccatt tgtgtatcgg tttaacggtt tttattattt atatccttca
     2101 acttcacagc gtggtgtacg tgcttatcgc agtattgacc tcatgaactg ggaaccagtc
     2161 acaggtgaag gactagaata cgggtatgta ttaagtcctg attacgatga aagcgttgtc
     2221 gcttatgcac cggaagttac ttattacgat ggttggtttt atttatgtga atcgcaagct
     2281 ggcactggtc attacttctt ccgttcgcgt tcaccagagg gcccttttgt gccttacact
     2341 ggtaatgttg gtgaatcaat tgatggtagt ttctttattg atgatgatgg taaggcttat
     2401 ttcttacgcg caaaagcaga aacaattaga attagacgtt ttaatgatga ctttacttta
     2461 tctaatgatg catttgatat tgataacaca tcaatgggcg gatggactga aggtccatat
     2521 gttttaaaac gcaatggcat ttattactta acatttactg gagttcatgt tggtagtcct
     2581 ggttataagg taggatatag ttacttacaa gattctgata ctgtttttaa taggtacgga
     2641 tatacacgcg cagaaaacat cttaatgaat acaaacgaag agtggcatac actgggtcac
     2701 agttctacga caatgggtcc tgatatggat tcgcattaca ttgcctatca ttcacgcaca
     2761 agcggggtat
//
LOCUS       DAOSEX010000100         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_35173_length_2756_cov_2.998519, whole genome shotgun sequence.
ACCESSION   DAOSEX010000100 DAOSEX010000000
VERSION     DAOSEX010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35173_length_2756_cov_2.998519"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PK612_02490"
     CDS             complement(<1..597)
                     /locus_tag="PK612_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOH95010.1"
                     /translation="MKTAMMTGHVTRFGDLEFALRLIKELGFEAVDLTMYDTFGGLAV
                     RNRELFTGDIIANAKRIKSIAEEINLPIVQAHTPFHVYRKDDEEYNAYMLETQKLCLQ
                     IAGIIGIPYVVIHPVMNADNEFNKKFFDELLPLAKKMNVVIATENMWEWQVGAIHCDY
                     STCSTPESFLSLLEYINHPNLKACVDVGHAQMFNGFDKS"
     gene            complement(620..1810)
                     /locus_tag="PK612_02495"
     CDS             complement(620..1810)
                     /locus_tag="PK612_02495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOH95011.1"
                     /translation="MFLQNLLETLIKLDVKTMMGVLICGNLVLSLLTLRYYIYHDSNY
                     DKTIIQRFGIAKILQAIAWLFLFFRGEISDVISVYFGNILLFVSFYLDSLIVLRLNDK
                     WTKGWVITQSVLLAVAVILFLVFEITLSAAHIRVGIASLAVFLVFAAPNVLSILNKES
                     TKFQKVIAAISLLFLIFLLLRAFNSLFDNEIVLFTNNFFQIGTFLALVLLMLTNGVGF
                     LLIMYDRSYALVKESSNLDPLTRIYNRRFFMNKAESYFKRAQQEDGYLSFLFIDIDFF
                     KKVNDTYGHLFGDEVLKVVAKTIAENIRPLDLCCRFGGEEFLIYLHDTSKEEAITIGN
                     RIREVVQLLEFKQVKEYKCTISVGVYSAKPNTETNLQQFIDNVDQALYKAKASGRNCV
                     MSFD"
     gene            complement(2408..>2756)
                     /locus_tag="PK612_02500"
     CDS             complement(2408..>2756)
                     /locus_tag="PK612_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95012.1"
                     /translation="LLLLFAVLVWTIFKFPKIVKRQKANTNNSRMYFAKKHYNHSGKT
                     RKQVLGKDNDLKVNELPLFNHPNKKECFVITKAMHDRIGKHKFIPLYKSGDNIAGVIL
                     TSKSSYGKKKTFK"
BASE COUNT          939 a          484 c          378 g          955 t
ORIGIN      
        1 acttttatca aaaccattaa acatttgcgc atgaccaaca tcaacacacg cctttaagtt
       61 aggatgatta atatattcta aaaggcttaa aaatgattca ggagttgaac aagttgagta
      121 atcacaatga attgctccta cttgccattc ccacatattc tcggttgcaa tgacaacatt
      181 catttttttc gctagcggaa gtagttcatc aaaaaacttt ttattaaact cattatcagc
      241 gttcatcact ggatgaataa caacataagg aatgccaatg atgcccgcta tttgtaaaca
      301 taatttttgt gtttctaaca tgtatgcatt atattcttca tcgtccttac gataaacatg
      361 aaacggggta tgtgcttgca caatcggaag attaatctct tcagcgatac ttttaatgcg
      421 tttcgcgttt gcaattatgt caccagtaaa taactcacga ttacgaacag caagaccacc
      481 aaaggtgtca tacattgtta aatcaacagc ttcaaaacct aactccttaa taagtcgtaa
      541 agcaaactct aaatcaccaa agcgtgtcac atggcctgtc atcattgctg ttttcataac
      601 tttgctcctc tatatttatt taatcaaaac tcattacaca attacgacca gaagcttttg
      661 ctttatataa agcttggtca acattatcaa taaactgttg gaggtttgtt tcggtgtttg
      721 gcttagcgga atatacaccg acactaattg tacatttata ttctttaact tgtttaaatt
      781 ctaatagttg cactacttcc cgaattctat taccgattgt gatcgcttct tctttactag
      841 tgtcatgtaa ataaattaaa aactcttccc cgccaaatct gcagcaaaga tctaatggtc
      901 gaatgttttc tgcgattgtc tttgctacaa cctttaatac ttcgtcccca aataagtgtc
      961 cataagtatc atttactttc ttgaaaaaat caatatcaat aaaaagaaat gataagtaac
     1021 catcttcttg ctgtgcccgc ttaaaatagg actcagcttt attcataaaa aagcgccgat
     1081 tataaatgcg tgttaacgga tctaaattag aactttcttt cactaatgca taagagcggt
     1141 catacattat aagtaaaaat cctacgccat tagttaacat taataacact aaggcaagga
     1201 atgtaccaat ttggaagaaa ttattagtga aaagtacaat ctcattatca aatagggaat
     1261 taaatgctcg taataagagg aagataagga agagcagact tattgcggca atgacttttt
     1321 gaaacttcgt gctctcttta tttaaaatac ttaaaacatt aggggcggca aaaactaaaa
     1381 ataccgctaa agaagcaatc ccaaccctaa tatgcgcagc gctaagcgta atttcaaaaa
     1441 ctaaaaataa gataactgct accgctaata aaacactttg tgttatcacc cagccctttg
     1501 tccacttatc atttagtcgt aaaacaatta acgaatctaa ataaaaactg acaaagagca
     1561 atatattacc aaagtaaaca gaaataacat cacttatttc ccctctaaaa aataaaaata
     1621 accacgcgat tgcttgtaaa attttagcaa taccaaatct ttggattatt gttttatcat
     1681 aatttgaatc gtgatatatg taataacgaa gtgttaaaag tgataacact aaattgccac
     1741 aaattaaaac acccatcatt gtttttacat ctagtttaat aagggtttct aataagtttt
     1801 gtaagaacat cttctattgg cctctttctt tttaactctg atcaatatgc atcctttttt
     1861 taatgcataa tcaaggcttt gtatatatta tacaaaaatt aatatctcgc gccttagaaa
     1921 ggtacaaaat agtgatattt ttttacaatt aaatgatatt tttataatta tttaatcaac
     1981 ttttacatgt tgttttatta caaaaaaata ttatttattc taaaacttga catttaacta
     2041 ataatatcta ataatgaaga tgcaagagga atcttggcgg tcttacccac taagtagtga
     2101 gagtgatgca ttcacgtaaa cctagggccg tattaaaggg atcagtatca gcatttacaa
     2161 tatgtattta aaccgcccct cattatttgc gggtaagcgg gctgatcgca tcattccagc
     2221 agttatttgg ataactgctt tttatttatg aactcattat atttttctaa gttcttttta
     2281 ttcgctttta aaaattgtaa atagttagat tcgtcatatg aaatattatt agaataacgt
     2341 aaatcttttt ttgttaaatt aatttttgca atacagtgat gaagttcatg tcctaaataa
     2401 ccacctttta tttgaaagtt tttttcttgc cataagaact tttacttgta agtattactc
     2461 cagcaatatt atctccgctt ttatataatg gtataaattt atgttttcca attctatcat
     2521 gcattgcttt agttattaca aaacactctt tcttatttgg gtgattgaat aatggtagct
     2581 cattaacttt taaatcatta tccttaccta atacctgttt tctagttttc ccactatgat
     2641 tataatgttt ttttgcgaag tacattctac tattatttgt attagctttt tggcgcttta
     2701 caattttggg aaacttgaaa atggtccata ctaaaacagc aaataacaaa agtaat
//
LOCUS       DAOSEX010000101         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_36079_length_2708_cov_3.354693, whole genome shotgun sequence.
ACCESSION   DAOSEX010000101 DAOSEX010000000
VERSION     DAOSEX010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36079_length_2708_cov_3.354693"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..247
                     /locus_tag="PK612_02505"
     tRNA            171..247
                     /locus_tag="PK612_02505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:205..207,aa:Arg,seq:tcg)
     gene            307..1863
                     /locus_tag="PK612_02510"
     CDS             307..1863
                     /locus_tag="PK612_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95013.1"
                     /translation="MKKIFKISLLLVATLLSACRSSSSSEHSEKLVSSSSEKTSEVTS
                     SSSESSSSTAFRILDTVKNIRIENNYLMWDAVTEATYYNVMVGSTGAEYERKTVYDAH
                     ELDLMTLSKVYTCATVVTITAHNSHAISVPSDAFTIICIDGVYYEFYSSPFEITYTNF
                     KVTDYELSWETNANFTEIKVGSDDPRVIPQTYISLPDYISGNAIHEFTFKGRVLLKEG
                     PSFTIKVISHNLKYYLYDDNLKLETPTLVRDDNIVGIIASGLYNNFAVTIGSHSFTTT
                     ASNFDLLDYAEYMTSEAFTVSVRAAYDNILYSEAATLNLVYIDGVIYHAGEEPRISGF
                     ALKNGKVLVWNKVDGVSEYSVQIGEYKKTVSTNEISLYNERNHIKRHTLVVIKAGTLS
                     GVCHINCDYETLSFSIEAAPKLNTPQGFNIEEKMYLTFARVPNDTANSLGMSSSYYLY
                     YNDGYSYETSSPAGASNTVSFNLGYISGWYTNPQVVRIVALGHSDWASSEAALVTINR
                     LNHDHYEIIY"
     gene            1948..>2708
                     /locus_tag="PK612_02515"
     CDS             1948..>2708
                     /locus_tag="PK612_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOH95014.1"
                     /translation="MNLAAQLREVLNNWFASFIANESLAQILVTAIMIIFWVILAYLV
                     TLVIKLIIFRTRRVERKLGVKETKEKQTVRRLINSLIRFFFLFWIIIMILKELGLDLV
                     PLLAGAGVVAFAVGFGAQELIKDVIAGMFLILEHTFKIGDFVEIGGQSGTVVDVGLRR
                     LKIQNWKGEVITINNGDIKVVMNASLNPSIAIIEFKMDYPFNVKELETADFRAFLKKF
                     KDKNPQVLEMPEKIVLMDVNDGLLFRVTIKTESRQ"
BASE COUNT          873 a          439 c          523 g          873 t
ORIGIN      
        1 aactttagag attgctgctg actacacctt taccggcgca gtcaaacttg cctagtaggc
       61 ataactaaaa taaattacca atcctaaatg gtttagcccg cttaacaaaa agcgggcttt
      121 tttaatagtc accttgaaat ttagtattat ttagattaat ctaaatatgt gtcccggtag
      181 ttcagttgga tagagcaaca gtcttcgaaa ctgtaagtcg ggcgttcgag tcgctcccgg
      241 gacgccacgc taaatataat gcttataaaa gcaaggtata taaatttaaa gaagggggta
      301 aacataatga aaaaaatatt taaaatatca ctattactcg tagcgacatt actttctgca
      361 tgtcgctctt cttcaagcag tgagcatagt gaaaagttag tctcttctag tagtgaaaaa
      421 actagtgaag ttacttcatc atcttctgaa tcttcttcat caacggcatt tcgcattctt
      481 gatacggtga aaaatattcg cattgaaaat aattatttaa tgtgggacgc tgtaactgaa
      541 gcaacttatt acaatgtgat ggttggttcc actggtgcgg aatacgaacg aaaaacagta
      601 tatgatgctc atgaattaga tttaatgact ctatcaaaag tatatacttg cgctactgtg
      661 gtaacgatta ccgcgcataa tagtcacgct atcagtgtcc caagtgacgc ctttacaata
      721 atttgtatcg atggtgtata ttatgaattt tattcatccc catttgaaat aacatacacg
      781 aattttaaag tgactgatta cgaactttcg tgggagacaa acgccaattt taccgaaata
      841 aaagttggca gtgatgatcc gcgcgttatt ccccagactt atattagtct tcctgattac
      901 ataagtggga acgctattca tgaatttaca tttaaaggcc gtgtactttt aaaagaaggg
      961 ccgtcattta cgattaaagt cattagtcat aatcttaaat attacttgta tgatgataat
     1021 ttaaaactag aaactcctac tttagttcgt gatgataata tcgtcggaat aatcgctagt
     1081 ggcttatata ataattttgc cgttactatt ggtagtcaca gttttacaac aaccgcatca
     1141 aattttgatt tactagatta tgctgaatat atgactagcg aagcatttac tgttagtgtt
     1201 agagcagcct atgacaacat actttatagt gaagcggcaa cacttaacct agtgtatatt
     1261 gatggcgtga tttatcacgc tggagaagaa ccgcgtatta gtggttttgc cttaaaaaac
     1321 ggtaaggtgc tcgtgtggaa taaagttgat ggtgtaagtg aatacagcgt acaaattggt
     1381 gaatataaaa agactgtttc cacgaatgaa ataagtttat ataacgagcg aaatcacatt
     1441 aaaagacata cgctggttgt tataaaagca ggtactttaa gcggtgtttg tcatattaat
     1501 tgtgattatg aaacgctaag tttttcaatt gaagcagctc ctaaattaaa cacaccacaa
     1561 ggttttaata ttgaagaaaa aatgtatctt acatttgcca gagtaccaaa tgataccgct
     1621 aattcgcttg gcatgagttc gtcttattac ctttattata atgatggtta tagttatgaa
     1681 acttcttcac cagcaggtgc aagtaacact gtttctttta atcttgggta tatttctggg
     1741 tggtacacta atccgcaggt agtgcgaatt gtggcgcttg gtcatagcga ttgggcaagc
     1801 agtgaagcgg cactagtgac gattaatcgc cttaatcacg accattatga aataatctat
     1861 taattaaact tttaattttc taacataaca ttggaaataa gtgacatatt tcgctaaaat
     1921 tacaagcgtt aatggaggta tattaatatg aatttagcag cacaattacg tgaagtatta
     1981 aataactggt ttgcgtcatt tattgcaaat gaatcacttg cgcaaatttt agtaacagca
     2041 attatgatta ttttttgggt gatccttgct tatttagtta cgcttgttat taaattaatt
     2101 attttccgca cacgccgcgt tgaacgtaaa cttggggtta aagaaacaaa agaaaaacaa
     2161 acagtgcgtc gccttatcaa tagcctaatc cgtttcttct ttttattctg gattattatt
     2221 atgatcttaa aagaattagg acttgattta gtgccccttc tagcgggtgc tggagttgtg
     2281 gcttttgccg ttgggtttgg tgcccaagaa ttaattaaag acgttatcgc tgggatgttc
     2341 cttattttag aacatacttt taaaatcggg gactttgttg aaatcggtgg gcagtctggc
     2401 actgtcgtag atgttggttt acggcgcctt aaaattcaaa actggaaagg tgaagtaatt
     2461 acgattaata atggtgacat taaagtagtg atgaacgcta gtcttaatcc aagcatcgct
     2521 atcattgaat ttaagatgga ttatcctttt aatgttaaag aattagaaac agcggacttt
     2581 agagccttct tgaagaaatt taaagacaag aatccacaag tacttgaaat gccggaaaaa
     2641 attgtcttaa tggatgtaaa tgatggttta ctctttagag ttacaatcaa aacagaatca
     2701 cggcaaca
//
LOCUS       DAOSEX010000102         2655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_37153_length_2655_cov_2.959231, whole genome shotgun sequence.
ACCESSION   DAOSEX010000102 DAOSEX010000000
VERSION     DAOSEX010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37153_length_2655_cov_2.959231"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2655
                     /gene="rpoC"
                     /locus_tag="PK612_02520"
     CDS             <1..>2655
                     /gene="rpoC"
                     /locus_tag="PK612_02520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOH95015.1"
                     /translation="TGAKFGEGAEVIKQLLKEVDLQKEFDEVNAALKTESKTKEKKLL
                     RRLEVINAFLKSGNRPEWMVLDVIPVIPPDLRPMLQLDGGRYATSDINDLYRRVISRN
                     NRYKALVQMNAPKVILMNGQRMLQEAVDSLIDNGRRGKPYTGANKRPLKSLSSSLKGK
                     QGRFRQNLLGKRVDYSGRSVIAVGPELKMYECGIPREMAIQLFRPFIAAELIKRDSRV
                     TNHTQADKIIDRHDEIVFDIIEEIIADHPVLLNRAPTLHRLGIQAFQPVLVSGRAIRL
                     HPLVCAGFNADFDGDQMAVHVPLSEAAQEEALNLMLASKNILGPKDGKPIAAPSQDMV
                     IGNYFLTVEESREDFVRRAEEARALGDEFGAQKFMQYAESEGKVFGSIDELMVAYQTN
                     SVHLHNRIALPGRAFNKTLFTPEMNKSYLVTSVGKVIFNTIFPNDFPYLNEPNKGSLQ
                     MDKIEYFVPAGTNIREHIASLPLRKPINQEFLGLIINEVFTRYGTVKTSAILDKIKDQ
                     GFHYSTKAGLTVSLHDITPIEGKEELVEAGEVEVRKINKAAARGFYSEKERYNAVINV
                     WKGVKDDVEAKLKEQFPLDKRNPLYIMFDSGARGKTTNFLQLKGLRGLMASPRGGEIE
                     IPIKSNFREGISVSEFFIASHGSRKGGADTAIKTADSGYLTRRLVDVAQNVIVTEEDC
                     GSDHGFVVTEIRIRENDTPAVPLSDRIAGRYTVHDIIHPTTGEVIIEANTLINKDAAL
                     YIEKAGVKEVEIRSLFGCEAPSGICAHCYGTNLATGERVKVGEAVGIMAAQSIGEPGT
                     QLTMRTFHSGGVAQVEDVTQGLPRVQELFEARNPKGKAKITEIAGEVVSITQEEDRRY
                     TVTVKNDLEEKSYLTEYGQQLRVKEGDTL"
BASE COUNT          833 a          535 c          597 g          690 t
ORIGIN      
        1 actggcgcta agtttggtga aggtgcggaa gtcattaagc aactcttaaa agaagttgac
       61 ttacaaaaag aatttgatga agttaacgct gcacttaaaa ccgaaagtaa aacaaaagaa
      121 aagaaattat tacgacgctt agaagtaatt aatgctttcc ttaaatcagg taaccgccca
      181 gaatggatgg tgcttgatgt tattcccgta attccaccag atttacggcc aatgttacaa
      241 cttgatggcg gcagatacgc gactagtgac attaacgact tatatcgccg tgtcattagc
      301 cggaataacc gctataaagc cttagtgcaa atgaatgcgc caaaagttat tcttatgaac
      361 gggcaacgga tgctccaaga agcagttgat agtctcattg ataacggccg tcgcggcaaa
      421 ccttacactg gcgcgaataa acgtccactt aaatcattaa gctcaagctt aaaaggtaaa
      481 caagggcgtt tccgtcaaaa cttactcggg aaacgcgttg actatagtgg gcgtagtgtt
      541 attgctgtcg gtcccgaact taagatgtat gaatgtggta tcccgcgcga aatggcgatt
      601 caattattcc gcccatttat tgctgcagaa ctcattaaaa gagattcacg cgtcacaaat
      661 catacacaag ccgataagat tattgatcgt catgatgaaa ttgtcttcga tattattgaa
      721 gaaattatcg ctgatcaccc agtattactt aaccgtgcac cgacgctgca ccgtttaggg
      781 attcaagcgt tccaacctgt gcttgtcagt ggtcgtgcga ttcgtttaca cccacttgtg
      841 tgtgcgggct ttaacgcaga ctttgacggc gaccaaatgg ccgtccacgt cccgcttagt
      901 gaagctgcgc aagaagaagc gctaaactta atgcttgcga gcaaaaacat tttagggcca
      961 aaagatggta aaccaattgc cgccccgtcg caagacatgg tgattggtaa ctacttctta
     1021 accgttgaag agtcacgcga agactttgtg cgtcgcgccg aagaagcacg agcgttaggc
     1081 gacgaatttg gtgcgcaaaa gtttatgcaa tatgctgaaa gcgaagggaa agtatttggt
     1141 tcgattgatg aattaatggt tgcttatcaa acaaatagtg tccacttaca taaccgtatc
     1201 gcgcttcctg gccgagcatt taacaaaaca ttatttacac cagaaatgaa taagtcttat
     1261 cttgtgacaa gtgttggtaa agtcattttt aatacaattt tccccaatga cttcccatac
     1321 ttaaacgaac caaataaagg ctcattacaa atggataaaa ttgaatactt tgtgcccgct
     1381 ggtacaaata ttcgcgaaca cattgcaagt ttaccgctgc gtaaacctat taaccaagaa
     1441 ttcttaggtt taattattaa tgaagtgttt acgcgttatg gcacggtgaa aactagtgca
     1501 attcttgaca aaattaaaga ccaaggtttc cattattcaa caaaagctgg tcttactgtt
     1561 tcactccacg acattacccc aattgaaggt aaagaagaac tcgttgaagc tggcgaagtt
     1621 gaagtgcgga aaattaataa agccgcagcg cgtggattct atagtgaaaa agaacgctat
     1681 aatgccgtca ttaacgtctg gaaaggtgtt aaagatgacg ttgaagctaa acttaaagaa
     1741 cagttcccac ttgataaacg taatccgctc tacatcatgt ttgatagcgg agcaagaggt
     1801 aaaacaacaa acttcttaca acttaaaggg ttacggggct taatggcttc gccgcgtggt
     1861 ggcgaaattg aaattccgat taaatcaaac ttccgtgaag gcattagcgt gagcgaattc
     1921 ttcattgctt cgcacggttc acggaaaggt ggtgcggata ccgcgattaa aactgctgac
     1981 tcagggtact taacccggcg gttagtcgac gttgcccaaa atgttatcgt caccgaagaa
     2041 gactgtggta gtgatcacgg ctttgtcgtt accgaaattc gtattcgtga aaatgacacc
     2101 ccagcagtgc cacttagtga ccgtattgcg ggtcgctata cagtccatga catcattcac
     2161 ccgacaactg gtgaagtaat tattgaagcg aatactctta ttaataaaga cgctgcgctt
     2221 tatattgaaa aagcgggtgt taaagaagtt gaaattcgtt cgctctttgg atgtgaagcg
     2281 ccaagcggaa tttgtgcgca ctgttacggc acaaacttag cgactggtga acgtgttaaa
     2341 gttggtgaag cggtcggaat tatggcggca caatcaattg gtgaaccagg aacacaatta
     2401 acaatgagaa cgttccacag cggtggtgtt gcgcaagttg aagacgtcac ccaagggtta
     2461 ccacgggttc aagaattatt tgaagcccgt aatccaaaag gtaaagcaaa aatcaccgaa
     2521 attgctggtg aagttgtaag tatcacgcaa gaagaagacc gtcgctatac cgttactgtt
     2581 aaaaacgatt tagaagaaaa gagttattta acagagtatg gtcaacaatt acgtgttaaa
     2641 gaaggcgaca cactt
//
LOCUS       DAOSEX010000103         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_37283_length_2648_cov_3.610104, whole genome shotgun sequence.
ACCESSION   DAOSEX010000103 DAOSEX010000000
VERSION     DAOSEX010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37283_length_2648_cov_3.610104"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PK612_02525"
     CDS             <1..802
                     /locus_tag="PK612_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOH95016.1"
                     /translation="SVSEARVLFEKSRGYDKKDATSVMNVPSKTEKEATLKGKKVAVL
                     EPIFALMKNKLLIKEFHNLVDKLRQAGAVVDFVPFDKDLLAAIFPTYFILSCAEANVS
                     NACLTGIGFGNKEEGASYEEIVANTRGKGFSSRIKYRLMLGQYALKKENRNEYYDRAQ
                     KARRLIVEAVDKVLENYDVIMLPAAPNHAPTVDKQSEPHSEIENNFMAIANFGGNPSL
                     TLPYFLDEGLPLGVNLTGKPFDDFNVLNIALKIEEITGLYNLHAGLKS"
     gene            799..2226
                     /gene="gatB"
                     /locus_tag="PK612_02530"
     CDS             799..2226
                     /gene="gatB"
                     /locus_tag="PK612_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOH95017.1"
                     /translation="MKYEVIIGLEIHIETKTNTKMFSSASSLFDAPANSNVSVVDMAF
                     PGTLPTVNKKAVQNAIMLGHALNMEIDDELHFDRKNYFYSDLPKGYQITQQRRPIGSN
                     GYLDIYVNDTKKRIRIERIHMEEDTAKQIHLGEVTLIDYNRAGNPLVELVTEPDLRSA
                     DEAMAFVEAVREIVTFLEISDGKMEEGSLRCDVNISLRPVGVSKFGNKVEIKNMNSIS
                     NIGKAIETEIKRQSELLDKGLEVESETRRYDDALKDTITMRKKDESVDYRYIPEGNIL
                     PISLSKEFIDATIREMKELPSVKRERYFEEFKLNAHEVNILLQDKDLTSYFDEAARDV
                     KNYKTLINWVIGEFSSYLNKEGKNAKSVALSPQNTSKLVNLIDNGTISNSQARELFAH
                     IIETNEDPEIKAKALNLMQVSDTDFILSVINEVINENLNVIDDIKAGKDRAMGFLVGQ
                     VMKKTKGKVNPALASKLMKQEIDKR"
     gene            2284..>2648
                     /locus_tag="PK612_02535"
     CDS             2284..>2648
                     /locus_tag="PK612_02535"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOH95018.1"
                     /translation="MKKKKIIRQITDNALIAAIYYVLTIILGGFAFNDVQFRISEILL
                     FLIFFRKDFVIGVTLGCLLANLHSLLQPWDVIFGTLATLLSGVLIAFSKRLYWGVIWP
                     TIINAVVVGLMLHIILDLP"
BASE COUNT          921 a          402 c          538 g          787 t
ORIGIN      
        1 cagtgttagt gaagcgcgtg tactttttga aaagtcgagg ggttacgata aaaaagatgc
       61 aacttcagta atgaatgttc cctcaaaaac agagaaagaa gcaactttaa agggcaaaaa
      121 agttgcagta ttagaaccta tttttgcgtt aatgaaaaat aaattattaa ttaaagaatt
      181 tcataatcta gtcgataaac ttcgccaagc gggagcagtt gtggactttg ttccgtttga
      241 taaagacctg ctagcggcga tatttccaac ttactttatc ttaagctgtg ccgaagctaa
      301 tgtaagtaac gcatgtttaa cagggattgg ctttggtaat aaagaagaag gcgcaagtta
      361 tgaagagatc gttgctaata caaggggcaa aggcttttct tcacggatta aataccgttt
      421 aatgttagga caatatgcgc tgaagaaaga aaataggaat gaatattatg accgtgccca
      481 aaaagcacgt cgcttaattg ttgaagcagt ggataaagta ttagaaaatt atgatgtcat
      541 catgctcccc gctgcaccga atcacgctcc aacagtagat aaacaaagtg aaccacatag
      601 tgaaatcgaa aataacttta tggctattgc taattttggt ggtaatcctt cacttaccct
      661 tccttacttt ttagatgaag gtttgccgct tggtgttaat ttaactggta aaccgtttga
      721 tgactttaat gtgttaaata ttgcacttaa aatcgaagaa ataaccgggc tttacaactt
      781 acacgcgggg ttaaaatcat gaaatacgaa gtaattattg gtttagaaat tcatattgaa
      841 acaaaaacaa acacaaagat gttttctagc gcttctagtc tttttgatgc acctgcgaac
      901 agcaatgtta gtgttgttga tatggctttt cctggcacat taccaacggt aaataaaaag
      961 gcagtgcaaa atgcaattat gttaggtcac gctttaaata tggaaattga tgatgaatta
     1021 cattttgatc gcaaaaatta tttttatagt gatttaccaa aaggttatca aatcacccaa
     1081 caaagacggc caattggtag taatggttat ttagatattt atgttaatga cactaaaaaa
     1141 cgcattcgga ttgaacgcat ccacatggaa gaagataccg ctaaacagat tcaccttggt
     1201 gaagttacac ttattgacta taaccgcgct ggtaatccgc ttgtggaact tgtcacagaa
     1261 ccagacttaa gaagtgctga cgaagcaatg gcgtttgtgg aagcagtgcg tgaaatcgtc
     1321 acattcctcg aaataagtga cggaaaaatg gaagaaggat cactacgctg tgatgttaat
     1381 atttcacttc gccctgtggg agtttcaaag tttggtaata aagtagaaat taaaaatatg
     1441 aactcaattt ctaatattgg caaagcaatt gaaacagaaa ttaaacggca aagtgaatta
     1501 cttgataaag ggcttgaagt tgaaagtgag acacggcggt atgacgatgc ccttaaagat
     1561 acaattacaa tgcgtaaaaa agatgaaagc gttgattatc gatatattcc tgaaggaaat
     1621 attttaccaa tttctttaag caaggaattt attgatgcaa caattaggga aatgaaagag
     1681 ttaccgagcg taaagcgtga acgctatttt gaagaattta aacttaacgc tcatgaagta
     1741 aatatcttgc tacaagataa agatttaaca agttattttg atgaagcagc gagggatgtt
     1801 aaaaactata agacactaat taattgggtt attggtgaat ttagttcgta cttaaataaa
     1861 gaagggaaaa acgctaaaag tgtagcatta tccccgcaaa acacctctaa attagtgaat
     1921 ttaatagata atggcacgat ttcaaatagc caagcgcgtg aattatttgc gcacattatt
     1981 gaaactaatg aagaccccga aataaaagcg aaagcactta atttaatgca agttagtgat
     2041 actgacttta tccttagtgt tattaacgaa gtaattaatg aaaatttaaa tgtaattgat
     2101 gacattaaag caggtaaaga tcgggctatg gggttcttag tcggacaagt tatgaaaaag
     2161 acaaaaggaa aagttaatcc cgctcttgct agcaaactga tgaagcaaga aattgataag
     2221 cgctaagcaa gcggagttga ggtgttcctt gcctaaccac caccatcaaa acaaggagaa
     2281 aatatgaaga agaaaaagat tattcggcag attacagata atgcgctgat tgctgccatt
     2341 tattatgtat taacaattat tcttggcgga tttgctttta atgatgtgca atttcggatc
     2401 tcagaaatat tattattttt aatatttttt agaaaagact ttgtcattgg tgttacactc
     2461 gggtgtttgc tcgctaattt gcatagtctg cttcaaccgt gggatgttat ctttgggacg
     2521 cttgctactt tattaagtgg agtgttaatt gcttttagta aaagactata ttggggtgta
     2581 atatggccga caataattaa tgctgttgtc gtcggactaa tgttacacat tattctcgat
     2641 ttaccatt
//
LOCUS       DAOSEX010000104         2635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_37583_length_2635_cov_2.427519, whole genome shotgun sequence.
ACCESSION   DAOSEX010000104 DAOSEX010000000
VERSION     DAOSEX010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37583_length_2635_cov_2.427519"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..405)
                     /locus_tag="PK612_02540"
     CDS             complement(238..405)
                     /locus_tag="PK612_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95019.1"
                     /translation="MSVVLLALLKNFAVMPNVDEPAKALEAYKLTGGAVVALVLAGLG
                     GVAGVAADLLK"
     gene            897..1853
                     /locus_tag="PK612_02545"
     CDS             897..1853
                     /locus_tag="PK612_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH95020.1"
                     /translation="MLTRVIGLDIGAATMRAAVVDREGTIYYSKEVATPERSELRFVR
                     EINDLLENVLFHAPEAIAIGVGVPGIVSPGTTLIHLLPNLGYENIDFKRILRKEIKQP
                     VYLLNDANAACYAEALFGAGADKRIVQYITLSTGVGGGLVINKEIYTGARGFALEIGN
                     MIIDTETPRPNDKMNRNSFEGSCSGKSLYNQAKRFGTNIHTVASVFNESRFSPIRNVW
                     VDNLGKGIANIYSLYDPDVVILGGAVMQSADKFFDDILPAVEKYTLPQLHPYINLKRS
                     HFGETGGMIGAALYALHEHEKIINKAKSDEVKIDTEVKAETK"
     gene            complement(1854..>2635)
                     /locus_tag="PK612_02550"
     CDS             complement(1854..>2635)
                     /locus_tag="PK612_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95021.1"
                     /translation="LIMKKLLIADLRRIFKGKGIYVLMILGFIFPAISALIVQLSGLA
                     ANDVAELGDLAAEVFNPVALYAGSFNPTQNIGLILLIVVAVLISGEFTNNTIRNKIVA
                     GHSKTSLFLSSLVTVLTYVFVVVLVNSSFTYLFNSISFGLQPFSDYFKRGLVAYSSIF
                     VIYTIMTLLTYQFKNLAGPLGLVLGIYFVLMMISTIVFSFNEGTGFKAKVLFYIFPFL
                     YLSGIATPIKLEWLWATLTNVGYLALLTTLGILLANRTDYK"
BASE COUNT          951 a          464 c          475 g          745 t
ORIGIN      
        1 aacaacaatt gacgccgata aaccgcgtgc cgcccattta gcgctcgtga gtttattctc
       61 taaaattaaa gatagtgaag atactgaagt aactggaaaa atagttgatg ttacacttgt
      121 tgaaggtaac acaactaaaa tgcgaaaata agtccttttt gccgcaaata attaataaaa
      181 atataaaaaa agataacgcg acgaagcgtt atctttcatt ttaattaaaa attaatctta
      241 ttttaaaaga tcagcggcaa caccagcaac accaccaagt ccagcaagga ctaaagcgac
      301 gacggcacca ccagtaagtt tataagcttc tagtgctttt gctggttcat caacatttgg
      361 cataaccgca aagtttttaa gaagtgctag taatacaaca ctcactaagc caagcgctaa
      421 aatgacaaag ccaagaagtt tatttttttc taaactttta acgactaaac ggccaacact
      481 aagtccaacc ccagcaagga gaagtaataa gactaagaag tttaagaaac taaacttaaa
      541 aataataact tctactccaa ggagcgtttc agttcttcca aaggctaaat caaggaaggt
      601 gtaacttact tctggttgag ttctagcaat cgcaggaacg gcaaaccata agatgagggc
      661 aactaaaaat aaaggacttt atcttgcttt taatttagtg ctaatattag taattcaaga
      721 ctaaatgtaa agattatgaa tgcttcgcat aaccctttac catcactaag gcacaattat
      781 gataaaatta ttacgatgaa ttattcataa ggaaagaaat tcatagttac tactcttaca
      841 tttctttagt gttgaattaa tagtagtaat aatgaattta gaaaggacaa attaatatgt
      901 taacacgagt tattggatta gatatcggtg ccgctactat gcgtgccgcg gttgtggacc
      961 gtgagggaac aatttattat agtaaggaag ttgcaacacc agaacgcagt gaattacgct
     1021 ttgtgcgaga aataaatgat ttattagaaa atgttttatt tcatgcccct gaagcaatcg
     1081 ccatcggtgt tggtgttcct ggtattgtta gccccggcac tacattaatt catctcttac
     1141 caaacttagg ttacgaaaat attgacttta aacgaatact ccgtaaagaa ataaagcaac
     1201 ctgtctattt acttaacgac gctaacgcag cgtgttatgc tgaagcactt tttggtgccg
     1261 gtgctgataa acgcatcgtc caatatatta ctttatcgac cggtgttggc ggtggacttg
     1321 ttattaacaa agaaatttat acaggagcgc gtggctttgc tttagaaatc ggcaatatga
     1381 ttattgatac agaaacaccg cgccctaatg acaaaatgaa tcgtaatagt tttgaaggga
     1441 gttgttcggg taaaagttta tataaccaag caaaacgctt tggcacgaat attcataccg
     1501 tcgcaagtgt gtttaacgaa agccgtttct ccccgattag aaatgtgtgg gttgataatt
     1561 tagggaaagg tatcgctaat atttattcac tctatgatcc tgatgttgta attcttggtg
     1621 gtgctgtgat gcaaagtgcg gataaattct ttgatgatat tttaccagca gtcgaaaagt
     1681 atacgctacc gcaacttcat ccgtatatta atttaaaacg gtcacacttt ggtgaaacag
     1741 gcgggatgat tggtgccgcg ctttatgctc ttcacgaaca tgaaaaaata attaacaaag
     1801 caaaaagtga cgaagtaaaa atagacactg aagtaaaagc agaaactaaa taattattta
     1861 taatcagtgc gattcgctaa taaaatacca agtgttgtaa gcaaggctaa atacccaaca
     1921 ttcgttaaag ttgcccataa ccattcaagc tttattggtg tagcaattcc agataaatat
     1981 aaaaagggga atatataaaa taaaactttg gctttaaaac cagtcccctc attaaaagaa
     2041 aagacgatgg tactaatcat cattaacaca aagtaaatac ctagcacaag cccgagtggt
     2101 cccgctaggt ttttaaattg ataagttaat agtgtcataa tcgtatagat gacaaagata
     2161 ctactatacg caactaatcc gcgcttaaag tagtcactaa aaggttgtaa gccaaagctt
     2221 atgctattaa aaaggtaagt aaaggaacta ttaactaata cgacaacaaa aacatatgtt
     2281 aaaacagtaa ctaaagaact taagaaaagc gatgttttcg agtgtcctgc aacgattttg
     2341 ttccgaattg tattatttgt aaattctcca gatattaaaa cagcaacaac aatgagtaaa
     2401 attaaaccaa tgttttgtgt tggattaaaa ctaccagcgt ataaagcaac aggattaaat
     2461 acttccgctg ctaagtcacc aagttctgcc acatcattag cggcgagacc tgaaagctga
     2521 acgataagcg cactaatcgc tggaaaaata aaacctaata tcattaagac ataaatacct
     2581 ttacctttga agatccgccg taaatcagca attaataatt ttttcataat taggc
//
LOCUS       DAOSEX010000105         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_37719_length_2628_cov_3.068403, whole genome shotgun sequence.
ACCESSION   DAOSEX010000105 DAOSEX010000000
VERSION     DAOSEX010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37719_length_2628_cov_3.068403"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PK612_02555"
     CDS             <1..1008
                     /locus_tag="PK612_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95022.1"
                     /translation="GARITGEEEYGYCTEFILRIGAGEEVKKTFNEERFASVLNSQGN
                     SIVMVRDEDILKVHIHTLRPGTTLTYAQQFGEFVTLKIENMTEQHTHISDHGKDYFNT
                     NPQVTSASMEMKDYALISVASGEGLSDLFKEYRVDYVVKGGQTMNPSTEDFLAAIKKV
                     HAKTVFILPNNGNIILAATQAAEVTNEEGVVNVHVIPSKTIPQGLVAAMNFNPEVDTE
                     HNIEIMTEALDAVETGQITFAIKDTSIDGVEVKKDYYMGLQGKKILCSLPDKFDALRE
                     LLAGMIDELTAIVTIIYGEDVEESAAEELLAELEEKYSAVDFELVNGGQPVYSFIVGL
                     E"
     gene            1017..2324
                     /locus_tag="PK612_02560"
     CDS             1017..2324
                     /locus_tag="PK612_02560"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH95023.1"
                     /translation="MAKISNSSRIINALAKYDIHTFRDVIFHLPRRYLDMSYTDEFKM
                     NTNEKVTLKLKVTSAPLLIKPQRVAIIRFTAVSESGATYKIVAYNQRYILNAVKIGDL
                     ITVYGTINLRLLELTVIKLFPRVLSEEEQLSPLYSLPSEVGNHEFIRIVQKALKIESV
                     TSRVPRPLADKNGLISLNDALKRVHNPRDLNDVFEGLKALKYEEGLEYFIKLLLIKRA
                     NSELTNSKKTIVNLKDANEFVKRLPFKLTKDQLTAVREIGYDMNKETLMYRLLQGDVG
                     SGKTVVSFVALYINYLRGAQGVLLAPTESLAKQHYETLLSFFKDFDLRIELLIGALTP
                     SVKTKLKEDIQHGLVDIVVGTHALFSEDVFYPKLELVVIDEQHKFGVNQRNTLLNKGE
                     KADLLLLSATPIPRTLANVIYGDMDISTIESFLVGVVVKVSSA"
     gene            complement(2212..>2628)
                     /locus_tag="PK612_02565"
     CDS             complement(2212..>2628)
                     /locus_tag="PK612_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95024.1"
                     /translation="RLNQRLTALFQQRGSHVALILLTVSLLPGILRLHANDDLRLLQA
                     PEASISAMEKRVQTLTELQSSGRFFLIEGDTQEQLLQRSEQLGDWLHAQFPLASVDLI
                     TRYLPSQQRQQENFQLLKYPYRHKLHLQVCAVSVSR"
BASE COUNT          869 a          459 c          545 g          755 t
ORIGIN      
        1 ggagcgcgga tcactggtga agaggaatac ggttactgta ctgaatttat cttacgaatt
       61 ggcgctggtg aagaagtaaa gaaaacattt aatgaagagc gttttgcaag cgtcttaaat
      121 tcacaaggta actcaattgt gatggtacgc gacgaagata tcttaaaagt gcacattcat
      181 actttaagac cagggacaac tttaacttat gcgcaacaat ttggggaatt tgtcacgctt
      241 aaaattgaaa atatgacgga acaacatacg cacataagtg atcacggcaa agattatttt
      301 aacactaatc cgcaagtgac tagtgcaagc atggaaatga aagactacgc tttaatttct
      361 gtcgctagtg gtgaaggatt aagtgattta tttaaagaat atcgtgtaga ttatgtagtt
      421 aagggcggac aaacaatgaa tccgtcaacc gaagactttt tagcagcaat caaaaaggtg
      481 catgctaaga ctgttttcat cttacctaat aacggtaaca tcattttagc ggcaacccaa
      541 gctgctgaag tcactaatga agaaggtgtt gttaatgttc acgttattcc aagtaaaaca
      601 atcccgcaag gcttagtcgc agcgatgaac tttaaccctg aagttgatac tgaacataac
      661 attgaaataa tgactgaagc acttgacgct gttgaaactg gacaaattac ctttgcaatt
      721 aaagatacat caattgatgg tgttgaagtt aaaaaagatt attatatggg tttacaaggt
      781 aaaaagattc tctgttcctt acctgataaa tttgatgccc ttcgcgaact ccttgctggc
      841 atgattgatg aattaacagc aattgtgacg ataatttacg gtgaagatgt tgaagagagc
      901 gccgcagaag aattacttgc ggaacttgaa gaaaaatatt cggcagttga ctttgaatta
      961 gtaaatggcg gccaacctgt atattcgttt atcgtcggtc tcgagtagag gtagcgatgg
     1021 cgaaaataag taattcatca cgaattatta atgcattagc aaaatatgac atccacacat
     1081 ttagggatgt catttttcat ttaccgcgtc gttatttaga tatgtcgtac actgatgaat
     1141 ttaagatgaa cacgaatgaa aaagtgacac ttaaattaaa agtaacgagt gcaccacttt
     1201 taattaaacc gcaaagagtg gcgattattc gtttcaccgc tgttagtgaa agtggcgcaa
     1261 cttataaaat tgttgcctac aaccaacgct atatattaaa cgccgtcaaa ataggtgact
     1321 taatcacggt atacggcaca attaatttgc gacttttaga actaacagtt attaaactct
     1381 ttccgcgggt gctaagcgaa gaagaacagc tgagtccact ctatagttta ccaagtgaag
     1441 taggcaatca tgagtttata agaatcgtgc aaaaagcact taaaatagaa agtgtaactt
     1501 cgcgtgttcc gcgcccttta gccgacaaaa acggcttaat ttcgctaaat gatgccttaa
     1561 aacgtgtgca taatccgcgt gacttaaatg atgtctttga aggacttaaa gcacttaaat
     1621 acgaagaagg attagaatat tttattaagc ttttacttat taaaagggca aatagcgaat
     1681 taactaattc aaagaaaaca attgttaatt taaaagacgc aaatgaattt gttaaaagac
     1741 taccatttaa gttaacgaaa gatcaactaa ctgccgtgcg agaaattggt tacgatatga
     1801 ataaagaaac actgatgtat cgtttgcttc agggtgatgt tgggagcggt aaaacagttg
     1861 tgagttttgt cgcactctat attaattact tgcgtggagc ccaaggtgtc ttacttgcgc
     1921 caacggaaag tttagcgaaa cagcattatg aaacgctcct ttcattcttt aaggattttg
     1981 atttacgaat cgaattacta attggggctt taacgccaag tgttaaaacc aaattaaaag
     2041 aagatatcca acatgggtta gtcgatattg tcgttggtac ccacgcttta tttagtgaag
     2101 atgtctttta tcctaaactt gaactcgtgg tcattgatga acaacataaa tttggcgtta
     2161 accagcggaa tacactatta aacaaaggcg aaaaggctga tttactctta cttagcgcga
     2221 caccgatacc gcgcacactt gcaaatgtaa tttatggcga tatggatatt tcaacaattg
     2281 aaagttttct tgttggcgtt gttgtgaagg taagtagcgc gtaataaggt caacggatgc
     2341 taaaggaaat tgcgcatgca accaatcgcc cagttgttca ctgcgttgca gtaattgttc
     2401 ttgtgtgtcg ccttctatca gaaaaaaacg tccgctggac tgtaattcag tcaatgtttg
     2461 cacacgtttt tccatcgcac tgatgctcgc ttccggcgct tgtaataaac gcaaatcatc
     2521 gttggcatgt aaacgcagta tgccgggcaa taaagaaact gttaataata ttaacgcgac
     2581 atgactgcca cgttgctgaa ataatgccgt taaacgctga ttgagccg
//
LOCUS       DAOSEX010000106         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_37973_length_2617_cov_2.572209, whole genome shotgun sequence.
ACCESSION   DAOSEX010000106 DAOSEX010000000
VERSION     DAOSEX010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37973_length_2617_cov_2.572209"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PK612_02570"
     CDS             <1..1150
                     /locus_tag="PK612_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOH95025.1"
                     /translation="LFNEDYNVVGYDTDTKVVASLNRGILHIKDEDFARALARKKLMA
                     TSDITYAFQNSDLVIIATPTHFNEEKQTFDLSSIDETLQKIIASNRSPLVIIKSTVNV
                     GATRYFMEKYPSLKIFFVPEFLREGHALFDNYYPARIIVGYDKNDTNHRQIAIQIANL
                     FKKYTKSIDDVPLLITGTLEAEAIKLFANSYLALRVSFFNEIDTFALSKDLEVKDIIE
                     GVGLDSRIGTHYNNPSFGYGGYCLPKDSKQLKAQFNLIPEELISATVNANSVRKEFIA
                     NYIADIVKKSNNKDYVVGIYRLTMKKDADNMRFSSIIDVVRLLKEQNIKMIIYEPLLN
                     ETTYQGITLINNLETFKKESSLIVTNRMHSDLKDVTNKVFTRDVYYRD"
     gene            complement(1161..1457)
                     /locus_tag="PK612_02575"
     CDS             complement(1161..1457)
                     /locus_tag="PK612_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH95026.1"
                     /translation="MYCEKLITLRRNRGLTQQQLADALMLSQSMISKIERGITRLDPM
                     LAIRFADFYNVSLDYLFGRSERQLQISEKDIENLSEQDKNEMLAFIIKLINEKK"
     gene            complement(1627..>2617)
                     /locus_tag="PK612_02580"
     CDS             complement(1627..>2617)
                     /locus_tag="PK612_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH95027.1"
                     /translation="NPHLYPFKVKYCEDEVDSYFAMRKISVDTDEEGIKRIYLNNKIL
                     FNTGLLDQGYYHEGYLTPPDEATVINEIKVAKEMGFNVLRKHIKIEWERFYYHCDRLG
                     MLVWQDFVNGGEPYNFVTVHVPAVLNIHFKDTNYKRFARQNALGRELFYAEAKTTIEH
                     LYNYPSIVLWTIFNEGWGQFDSHKLLPFVLELDNTRLIDVNSGWHDMGLGQFRSHHVY
                     FRKYRFKKDKLDRCVILSEFGGYAHRIPGHYYGVANFGYRKYNTKEELEKAVINLYEK
                     EIIPAVKQGLAAAIYTQLSDVEDELNGVLTYDRKVVKIDPKVMRELNEKLLKA"
BASE COUNT          867 a          463 c          413 g          874 t
ORIGIN      
        1 actttttaat gaggactata atgttgttgg ttatgacaca gatacgaaag ttgtcgctag
       61 tttgaatcgc ggtattttac atattaaaga cgaagacttt gcgcgagcgc ttgctcgtaa
      121 aaaactaatg gcaacaagtg acataactta tgcattccaa aatagtgatc ttgttattat
      181 tgcgacacct acccatttta atgaagaaaa acaaacattt gatctctcat caattgatga
      241 aactttacag aaaattatcg caagtaatag gagtccgctt gttattatta aatcaacggt
      301 taatgttggc gcgactcggt attttatgga aaagtatcca tcattaaaaa tcttttttgt
      361 ccctgaattt ttacgcgaag gtcatgccct ttttgataat tattatccag cgcgaattat
      421 tgtcggctac gataaaaatg acacaaacca ccggcaaatt gctattcaaa ttgctaatct
      481 ctttaaaaaa tacactaaat ctattgatga tgtcccgctt ttaattacgg gcactttaga
      541 agctgaagca attaaattat tcgctaatag ttatttagcg ctccgcgtat ctttctttaa
      601 tgaaattgat acatttgcgt tatctaaaga cttagaagtt aaagatatta ttgaaggtgt
      661 tggcttagat tcgcgcattg ggacacatta caataatcct tcatttggtt atggtggtta
      721 ttgtttaccg aaagatagta aacaactaaa agcacaattt aatctaattc ccgaagaatt
      781 aatcagtgcg actgttaacg caaatagtgt gcgtaaagag tttatcgcta attatatcgc
      841 cgacattgta aaaaagagca ataataaaga ttatgtcgtt ggtatttatc gcctaacaat
      901 gaaaaaagat gctgataata tgcgcttttc atcaatcatt gatgtagtta gattactaaa
      961 agagcaaaac attaaaatga ttatttatga accgctttta aacgaaacaa cttatcaagg
     1021 tattacgctc attaataatt tagaaacatt taaaaaagaa agttcactaa ttgtcacaaa
     1081 ccgtatgcat agtgacttaa aagatgtcac taacaaagta ttcacccgcg atgtctatta
     1141 tcgcgattaa tatatcttca ttatttcttt tcgttaataa gtttaataat aaacgctaac
     1201 atctcatttt tatcttgttc tgataaattt tcaatatctt tttcgctaat ttgtaactgc
     1261 ctttcactac ggccaaacaa gtaatcaagt gaaacattat aaaaatctgc aaaacgaatt
     1321 gctagcattg gatcaagccg agttatgccg cgttctattt tagaaatcat tgattgcgac
     1381 aacattaaag catcggcaag ttgctgctgg gttaaaccac gattacgacg taaagtaatt
     1441 aatttttcgc aatacatgat tttatcttaa ctaaactaaa acgcttttac tttattaatt
     1501 gcttaatatt ctaatttaga ataaaatggc tttacaaata ttctgtaatg gaatattaaa
     1561 aagacattat caatgcataa ttggcaggaa ccgaatcgca aagataatgt cttttcttta
     1621 aatgggttaa gcttttaaga gtttttcgtt aagttcacgc ataacttttg gatcaatctt
     1681 aacaaccttt cggtcataag ttaatacacc atttaattcg tcttcgacat cacttaattg
     1741 ggtatatatc gccgctgcta ggccttgctt aactgcagga ataatttctt tttcatacag
     1801 gttaattacc gctttttcta attcttcttt agtgttatat ttgcgatatc caaagttagc
     1861 gaccccgtaa tagtgtccag gtatccggtg cgcataaccg ccaaattcac ttaggataac
     1921 gcagcgatca agcttatctt ttttaaagcg atatttacga aaataaacat gatgactgcg
     1981 gaactgtcca agccccatat catgccaacc tgaattaaca tctattagtc gagtattatc
     2041 aagttcaagc acaaacggga gtaatttatg actatcaaat tggccccagc cttcattaaa
     2101 tattgtccat aaaacaatgg acggatagtt atataaatgt tcaatcgttg ttttagcttc
     2161 agcgtagaat aattcgcgtc caagggcatt ttgccgtgca aaacgcttat aatttgtatc
     2221 tttaaagtga atatttaaca ctgctggaac atgtacagta acgaagttat acggttcgcc
     2281 accattaaca aaatcttgcc acactagcat ccctaagcga tcacaatgat aataaaagcg
     2341 ttcccattca attttgatat gtttccgtaa aacattaaag cccatctctt ttgcgacttt
     2401 aatttcatta ataactgttg cttcatcggg tggtgttaaa taaccttcgt gataatagcc
     2461 ttgatcgagt aatcccgtat taaaaagtat cttattattt aaatatatgc gtttaatccc
     2521 ttcttcatct gtatcaacac taattttacg catcgcaaag tacgaatcaa cttcatcttc
     2581 acagtatttc actttaaagg gatagagatg cgggtta
//
LOCUS       DAOSEX010000107         2583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_38722_length_2583_cov_2.518987, whole genome shotgun sequence.
ACCESSION   DAOSEX010000107 DAOSEX010000000
VERSION     DAOSEX010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38722_length_2583_cov_2.518987"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..839)
                     /locus_tag="PK612_02585"
     CDS             complement(60..839)
                     /locus_tag="PK612_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003323489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOH95028.1"
                     /translation="MAKIIAIANQKGGVGKTTTAINLSASFAHFGRKVLLIDMDPQGN
                     SSRGLGLDPATIKNTIYHTFFKGININKAIYRTPLKSLDIIGANLFLARTDVEISTIP
                     QHPFTVLQSKIKEIDRRYDYIFIDCPPSLGLLSTNALVAANSVIIPVQCEYFALEAVA
                     QVLATIKQVQASHNSELKIEGFLLTMYDERTRLGIEISQQIRGLFKESTFMTPIPRNI
                     ALAEASAKMLPTILYKPTSKGSLAYLNVAKEIMENEERAEE"
     gene            complement(873..1574)
                     /gene="rsmG"
                     /locus_tag="PK612_02590"
     CDS             complement(873..1574)
                     /gene="rsmG"
                     /locus_tag="PK612_02590"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOH95029.1"
                     /translation="MKNNPFLQYLEEHFSENAEVLKVQFAKYAALIEEHNKVQNLTAI
                     APSEYEEKHFLDCLLLRETYNFRNETLLDIGSGAGFPGVVLAIAYPELQITLLEPIKK
                     RCNFLSTVVKELELTNVEVVNERAEDYAASKRGEYDLVTSRAVSSLSIMLELSVPFLR
                     INGSALMMKGRNYNAEFSDAENAIYILKVEVGKTDLHFLPSDGSMRVNARFIKKGSTP
                     KKYPRPFAQIKKNPL"
     gene            complement(1564..>2583)
                     /locus_tag="PK612_02595"
     CDS             complement(1564..>2583)
                     /locus_tag="PK612_02595"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH95030.1"
                     /translation="KERHQLFIEPESRYTDSMYLQGLSTSMPVEVQEKMIRSLKGLEN
                     VEILKYGYAIEYDAIDPLELKPTLELKKWDNLYCAGQIIGTSGYEEAAGLGLLAGINA
                     VLKIDNKPPLILKRDEAYIGVMIDDLVTKGTEEPYRLLSSRSEYRLLLRHDNADSRLT
                     EIGRQVGLVKDDRYAKFLKSQKAIADARAILETTQVSADIPVVEFLESVGYPNYSGGL
                     TIAELIKRPRVTLDDLLELLGHLTLEGLNHNDLFQLETDIKYEGYINKQRQEAKRLKK
                     LEEVEIPASFTYDDLDGLRIEARQRLAKVKPLTIGQAKRISGVNPNDIIILMTYLRRY
                     GKNEK"
BASE COUNT          772 a          514 c          418 g          879 t
ORIGIN      
        1 ctaaaacatt tgattgcgaa tatttagaaa ttaaatctga aaggtgttct tttaacaaat
       61 tattcttcgg ctcgttcttc attctccata atctccttag ctacatttaa gtaggctaat
      121 gaacctttcg aagtcggttt atataaaatg gttggcagca ttttagcact tgcttccgct
      181 agagcgatat tccgcgggat tggtgtcata aatgttgatt ctttaaatag acctctaatt
      241 tgttggctaa tttcaattcc tagtctagtt ctttcgtcat acatcgttaa cagaaaacct
      301 tctattttca gttccgaatt atgtgaggct tgaacttgtt taatcgtcgc taaaacttgg
      361 gcaacagcct caagcgcaaa atattcacat tgcacaggaa taattacact attagccgcc
      421 actaaagcat tagtacttaa tagtccaagt gaaggcgggc aatcaataaa tatataatca
      481 taacggcgat caatttcttt aatttttgat tgtaaaacgg taaacgggtg ttgcggaatc
      541 gtgctaattt ccacatcagt ccgcgccaga aaaagattag cgccaataat atctaaactt
      601 tttagtggtg tgcgataaat agctttatta atattaatgc ccttgaaaaa agtatgataa
      661 atcgtatttt taatcgtcgc aggatcaagc ccaagcccac ggctactatt gccttgcggg
      721 tccatatcaa ttaaaagtac cttgcgccca aaatgtgcaa aagaggcact taaattaatt
      781 gcggtcgttg ttttgccaac accgcccttc tgatttgcaa ttgcgatgat tttagccata
      841 ctattccttg taataagtat atcaaatata ctttaaagtg gatttttctt aatttgtgcg
      901 aatggacgcg gatatttttt aggagtactt ccctttttaa taaagcgagc gttgacccgc
      961 atactaccgt cacttggtaa gaaatgtaaa tctgtttttc caacttcaac tttaaggatg
     1021 taaattgcat tttcagcgtc actaaattca gcattataat ttcgcccctt catcattaaa
     1081 gcactgccat tgatgcgtaa aaacggtacg cttagttcaa gcattatact tagcgaagac
     1141 acagcgcgac ttgtgactaa atcatattca ccgcgtttac ttgcggcata atcttccgca
     1201 cgttcattaa caacttcaac atttgtaagt tctaattctt taacgacagt acttaagaaa
     1261 ttacaacgct tcttaattgg ttcaagtaaa gttatttgta attcaggata agcaatcgct
     1321 aacacaactc ctggaaaccc tgcaccacta ccgatatcta ataatgtttc attgcggaaa
     1381 ttatatgttt cgcgaagtaa taagcaatct aaaaaatgct tttcttcata ttcgcttggg
     1441 gcaattgcgg ttaaattttg taccttatta tgctcctcga taagtgcagc atactttgca
     1501 aattgcactt ttaacacttc agcattttcg ctaaaatgct cttctaagta ctgcaaaaat
     1561 gggttatttt tcatttttac catacctcct caaatatgtc attaatataa taatatcgtt
     1621 aggattaaca ccactaatac gtttagcttg accaattgta agtggtttaa ctttagctaa
     1681 gcgctgccgc gcttcgatcc gcaaaccatc taaatcatca taagtgaaag aagcaggaat
     1741 ttctacttct tcaagcttct ttaaacgctt tgcttcttgg cgttgcttat taatatatcc
     1801 ttcatatttt atatcagttt ctagttgaaa taaatcattg tggttaagac cctcaagtgt
     1861 taagtgtcct aaaagctcta ataaatcatc tagtgtaact cgcggtcttt taataagttc
     1921 agcaatggtt aatccgcccg aataattagg ataaccaaca ctttcgagaa attcaacgac
     1981 aggaatatcc gcgcttactt gtgttgtttc taaaattgct cgagcatccg ctatggcttt
     2041 ttgacttttt aaaaacttcg cataacgatc atcttttact aagccaactt gtcgcccaat
     2101 ttcggtaagg cgcgaatcag cgttatcatg tcttaagagt aagcggtatt cagatctact
     2161 tgataataaa cgatatggct cttcagtgcc ttttgtcact aagtcatcaa tcataacacc
     2221 aatgtaggcc tcatctcttt tcaaaattaa gggtggctta ttatctattt tcaacacagc
     2281 attaattcct gctaaaagac caagtcccgc tgcttcttcg tatccacttg tgccaataat
     2341 ttgaccagcg caatataagt tatcccactt tttaagttct agtgttggtt taagttcaag
     2401 tggatcaatt gcatcatatt caatcgcata accatatttt aatatttcaa cattttctaa
     2461 accttttaga ctgcgaatca ttttttcttg aacttcaact ggcattgaag tacttaatcc
     2521 ttgtaaatac attgaatcag tgtaacgtga ttctggttcg atgaataatt gatgacgttc
     2581 ttt
//
LOCUS       DAOSEX010000108         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_38981_length_2571_cov_3.173688, whole genome shotgun sequence.
ACCESSION   DAOSEX010000108 DAOSEX010000000
VERSION     DAOSEX010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38981_length_2571_cov_3.173688"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK612_02600"
     CDS             complement(<1..444)
                     /locus_tag="PK612_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95031.1"
                     /translation="MLIIDYIREKIKEFLGIQHIHKNPNDINFQYISDEEILRISKAK
                     EFKLWYLGEGDKLEQYYLLGESARKGVTDRNYFWSIATREAMVKKVHSGIPNAIINTL
                     VNVVGEHQITSDDKELERIIYDMLEDNDFIRMVNQEQLPLTLAQGW"
     gene            complement(502..1947)
                     /locus_tag="PK612_02605"
     CDS             complement(502..1947)
                     /locus_tag="PK612_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HOH95032.1"
                     /translation="MSNELSMAKLIGKGYSKAWWTNNHSRYRLFKGGRNTKKSYVMLG
                     LEVINKILTQPYRNVMIVRQTETSHKITTWNTIIGAIRQPDYRNPDISLSRYFKINSV
                     DKIITYIPTGQMIVFRGFDNADKLTGLRALTGFFTDVYIEEAFEIKSYDEFRKLDGSL
                     RGQLPEGYFYQITFLFNAWKKEHWLYDVFFKGRLEDDYNLLEANDYIDYYDSEYIGDY
                     GKGLYLHISTYKINEFRDGIYDVAMENLREKAPEIYKVEALGMWGVTGERVYPEWNDS
                     CIAEPQKVMATRFRRYAIGIDTGLSAAKLNKDQVKSATTMIMSGLTWDRKKIIAIDEY
                     FHSNAKVKKTEPEIMAEIIETLKLWQEKYRNHPDLMKGQVLVYVESADIGFKDGLQLL
                     AHKNGLINFIFQPSTKYGVEKRVYFIRTLMAYGDYLVSSLCPNLIREHRTATSGEKGE
                     VRAGNDHAQDGCEYGWASFVNELQLWATIKL"
     gene            complement(1937..2341)
                     /locus_tag="PK612_02610"
     CDS             complement(1937..2341)
                     /locus_tag="PK612_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95033.1"
                     /translation="MNGKKKKMGAKPILASNKEAIKVFLEGIQLGQTIKDSAEFANIA
                     PSSIYLWVEKGEADKEAGKKTIYSDFLESFNRAKQQFIRNGLYQIYQASKQDWRAAAY
                     LLKMRHPDTYGDRQDGRSTAEETGVKIINDVK"
     gene            complement(2343..>2571)
                     /locus_tag="PK612_02615"
     CDS             complement(2343..>2571)
                     /locus_tag="PK612_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOH95034.1"
                     /translation="IHPTQKPVEIPAKAIKNSSRRGGSVLDLFGGSGSTMIACEQLDR
                     ICYMMELDEKYVDVIIDRWEKYTGRKAVKVE"
BASE COUNT          684 a          580 c          403 g          904 t
ORIGIN      
        1 ccacccttga gcgagagtta aaggtaattg ttcttgattc accatacgga taaaatcatt
       61 gtcctcgagc atatcataaa ttattctctc aagttcttta tcatcgcttg tgatttgatg
      121 ttcgccaacg acattaacta aagtattaat gatagcgttt ggaatgccgg aatgaacttt
      181 cttgaccatc gcttctcgtg tggcaatcga ccagaagtaa ttacgatccg ttaccccctt
      241 acgagcagat tcgcctaata gatagtattg ttctaattta tcgccctcgc ctaaatacca
      301 gagtttgaat tcttttgctt ttgaaattcg caaaatttcc tcatcgctga tatattgaaa
      361 atttatatca ttcggattct tatgtatgtg ttgtatccct aaaaattctt taattttttc
      421 tctgatgtaa tcgataatga gcatttggtt tcccccgttt tcgtagttcc atacatttat
      481 tatatatcaa tttgcattaa actataattt tattgttgcc cataactgaa gttcgttcac
      541 aaaacttgcc cacccatact cgcaaccatc ttgtgcgtgg tcgtttcccg ctcttacttc
      601 tcccttttcc ccgcttgtcg cagttcggtg ttctcggata agattaggac atagtgaaga
      661 cactaaataa tccccgtaag ccataagtgt tcgtataaag taaacacgct tctcaacccc
      721 gtattttgtg cttggttgaa agataaaatt aatcaatcca tttttatgag cgagtagctg
      781 caaaccatcc ttaaatccga tatcagccga ttcgacatag actaatactt gccctttcat
      841 taagtcggga tgattacggt atttctcttg ccacagtttt aatgtttcaa taatctccgc
      901 cataatttct ggttcggttt tcttaacctt tgcattcgag tggaagtatt catcgatggc
      961 aataatcttt ttcctgtccc aagtcagtcc agacataatc atcgtggtcg cacttttcac
     1021 ttggtcttta ttaagttttg ccgcacttaa ccccgtatca atcccgatcg cgtaacgcct
     1081 aaatctcgtt gccattacct tttgcggttc agcaatacaa ctatcattcc actcgggata
     1141 aactcgttcg cccgttacgc cccacattcc taatgcttcg actttatata tctcgggtgc
     1201 tttttctctt aagttctcca ttgccacatc gtaaatgcca tcgcggaatt catttatctt
     1261 ataagtcgat atgtgtaagt aaagtccttt gccgtaatct ccgatatatt cgctgtcgta
     1321 gtagtcaatg taatcattcg cttcaagtag attgtaatca tcctctaacc gccctttaaa
     1381 gaaaacatca tagagccagt gttctttttt ccaagcattg aataaaaatg tgatctgata
     1441 gaaataaccc tccggcagtt gtcctcttaa tgatccatca agttttctaa attcatcata
     1501 agatttaatc tcgaacgctt cctcaatgta gacatcggta aaaaatccag ttaatgcccg
     1561 aagccccgta agtttatcag cgttatcaaa acccctaaat acgatcattt gcccagttgg
     1621 gatataggta atgattttat cgactgaatt gattttaaag tagcgagata atgagatgtc
     1681 cggatttcta taatctggtt gtctaatcgc tccaataatt gtattccaag ttgtaatctt
     1741 atggctcgtt tcggtttgcc gcacaatcat aacattacga tatggttgag taagaatttt
     1801 attaatcacc tcaagcccta acataacata agacttcttt gtgtttctcc cccctttgaa
     1861 taaacgatac cgagagtggt tatttgtcca ccacgcttta gaatatcctt taccgattaa
     1921 tttcgccatc gagagttcat ttgacatcgt tgataatttt tactcccgtt tcctctgcgg
     1981 ttgatctgcc atcttgtcta tcaccatatg tatcggggtg tcgcattttc aaaagataag
     2041 cagctgccct ccagtcttgc ttacttgctt gatagatttg atatagccca tttctaataa
     2101 attgttgctt cgcgcgattg aatgactcta aaaagtccga ataaattgtt tttttaccgg
     2161 cttctttatc agcttcacct ttttctaccc ataggtaaat gctacttggc gctatattag
     2221 caaattccgc actatcttta attgtttgac caagttgaat gccctctaaa aaaactttga
     2281 ttgcttcctt attggatgca agaatcggtt tagcgcccat ttttttcttt ttgccattca
     2341 tactactcaa cctttaccgc ttttctacct gtatactttt cccaacgatc aataattaca
     2401 tcaacatact tctcatcaag ttccatcata taacatattc tatcaagttg ttcacaagca
     2461 atcatcgtgc ttccactacc tccaaataag tcgagaacgc tacctcctct tcttgaacta
     2521 tttttaattg cttttgctgg tatttcaaca ggcttttgag ttggatgaat a
//
LOCUS       DAOSEX010000109         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_39793_length_2536_cov_2.596131, whole genome shotgun sequence.
ACCESSION   DAOSEX010000109 DAOSEX010000000
VERSION     DAOSEX010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39793_length_2536_cov_2.596131"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PK612_02620"
     CDS             <1..508
                     /locus_tag="PK612_02620"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOH95035.1"
                     /translation="FTSSIVLAQQGLIIGTKDGKDGVINARGDEVIPFIYDKIFDSEK
                     EGYMIANKSNMSYRIKLATNEATSLSSVVTKAGNEKTPLYTYFDASRLYPITVLNEKE
                     IVYQFRIEGEVSTYWQPTFMTFTQSAMPGYNVVLKAQVIIDTKIYYYFVHFEYVAHAY
                     KNNGYLWQ"
     gene            669..1544
                     /locus_tag="PK612_02625"
     CDS             669..1544
                     /locus_tag="PK612_02625"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HOH95036.1"
                     /translation="MKKCVFYTALSGLALTIFGLIVAFFGYRLDLFVEAIPHLVGLFP
                     ALWWVILPPAVGLLVLIINIAVKAKKKNKCAIVPFALFVSILAYLLVVPRALPEWLAE
                     GVAPESLVFTTGAAVVVIVFLVLFVLAFVDMALPTRKQLRAEKELDEEVYEVVEEKPT
                     RGPHDAPVLYSDEEVEDEESLEEVSEAERKVAEKPKAKKAKAKETEKAKEEEEEVKKE
                     DVKKEKEYDRIYHIMKRAKDDRWIVKIAQSRKAIKIFDTQKEAIAYAEILASNNNGVV
                     RVFASKGANKGRIIQ"
     gene            1613..2164
                     /locus_tag="PK612_02630"
     CDS             1613..2164
                     /locus_tag="PK612_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95037.1"
                     /translation="MAKIIIISGDLAAGKSVLAKRLSRHYQIPYFSKARFKEILGEKI
                     SFEGIDENKNISRSSYALLRHIGWRVAQVKGSVILEANFHTEHLEQIKRDVEFTGHKV
                     ELLYLTGDLDVLFERFLYREHYENRHPVHLTHPLRSLEEFENYILKLRNEPEVLPRKM
                     LDTTNCDQEMIFEKALKLIGSLD"
     gene            2180..>2536
                     /locus_tag="PK612_02635"
     CDS             2180..>2536
                     /locus_tag="PK612_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOH95038.1"
                     /translation="MSYTTDKIRNIIFLGHQGSGKTTLVEQIASNILKQPAGSVERKN
                     TISDFTPEEKARLTSCSMSVVSLDYNGYRLNLLDAPGNDDFVADVVGVLDFVKAAVLV
                     VDTGKIEVGTVKHFNLL"
BASE COUNT          867 a          418 c          512 g          739 t
ORIGIN      
        1 ttttacaagt agtatagttc tagcacaaca aggacttatt atcggtacaa aagacggtaa
       61 agatggagtg attaatgcgc gtggtgacga agtaattcca tttatctacg acaaaatttt
      121 tgatagtgaa aaagaaggtt acatgattgc taataaaagt aatatgtcgt atcgcataaa
      181 gttagccact aatgaagcca ctagtttaag cagtgtagtc actaaagcag gtaatgaaaa
      241 gacaccgctt tatacatatt ttgatgcaag tcgcctctac ccgattactg tattaaatga
      301 aaaagaaata gtgtatcaat ttagaattga aggtgaagtt agtacttact ggcaaccgac
      361 ttttatgaca tttacacaaa gtgcaatgcc gggatataat gttgtgctaa aagcccaagt
      421 tataattgat actaaaattt attattattt tgttcacttt gaatatgtag cgcacgccta
      481 taaaaataat ggatacttat ggcagtgaag ttttacaacc ttaaaaggaa agtaaagaat
      541 aaagcgctta ctactttaaa taaaagcaaa atggggcaaa tattcggtat tttagcccct
      601 ttttgtttac ttccgagtat tatttttata taatactatt gagttttata ttaggaaaag
      661 gagaacttat gaagaaatgt gttttttata ccgctcttag tggtctcgcc ttaacaatct
      721 ttggtctcat cgtggccttt tttggttatc ggcttgatct ctttgtagag gcaattcccc
      781 atttagttgg attatttccc gcattatggt gggtaatttt accgccagcc gtgggacttt
      841 tggtgttaat tattaatatc gctgttaaag ccaagaagaa aaataaatgt gcaattgttc
      901 cgtttgcgct atttgtgtct attcttgctt acttactcgt agtgccacgc gcattaccag
      961 aatggttagc agaaggcgta gcaccagaaa gtttagtctt tacgactggt gcagcagttg
     1021 tcgtcattgt atttttagtg ctctttgtct tagcgttcgt tgatatggca ctcccaacac
     1081 ggaaacaact tcgcgcggaa aaagaacttg atgaagaagt ttatgaagta gttgaagaaa
     1141 agccaacacg cggaccacat gacgctccag tactatacag tgatgaagaa gtcgaagacg
     1201 aagaaagctt agaagaagtt agcgaagctg aacgcaaagt cgcagaaaaa ccaaaagcga
     1261 aaaaagcaaa agcaaaagaa acagaaaaag caaaggaaga ggaagaagaa gtgaaaaaag
     1321 aagacgtaaa gaaagaaaaa gaatacgacc gcatttatca catcatgaaa cgggcgaaag
     1381 atgatcgttg gattgttaaa attgcccaaa gccgcaaagc aattaaaatc tttgatacac
     1441 aaaaagaagc gattgcatat gctgaaattt tagcgagtaa caacaacgga gttgttcgtg
     1501 tcttcgcctc aaaaggcgcc aataaagggc gcattattca gtagtaaaaa gaaggcactt
     1561 aacacaaggt gcctttttat atattgtgct aagtagaaag gaatattaaa gcatggccaa
     1621 aatcataatt atttcaggtg acctcgccgc tggtaaaagt gtgttagcaa aaaggctttc
     1681 acgtcattat cagatccctt atttctcaaa agctcggttt aaagaaatac tcggcgaaaa
     1741 aatttcgttt gaaggaatcg acgaaaataa aaatatttcg cgctcctcat atgcgctctt
     1801 acgccatatt gggtggcggg tagcccaagt aaagggcagt gttattttag aagctaattt
     1861 tcacactgaa cacttagaac aaattaaacg cgacgttgaa tttactggtc acaaagttga
     1921 gttgctctat ttaacgggtg atttagatgt actatttgaa agatttttat atcgtgaaca
     1981 ttacgaaaat cgccatccag tgcacttaac tcatccacta cgttcattag aagaatttga
     2041 aaattatatt ttaaaattac gtaatgaacc tgaagtatta ccacgcaaga tgcttgatac
     2101 cactaattgt gatcaagaaa tgatttttga aaaagcactc aaactcattg gtagtttaga
     2161 ttaatatgaa ggagaaatta tgagttatac cacagataaa attagaaaca ttatcttcct
     2221 tggccaccag ggctcaggaa aaacgacact tgtggaacaa atcgcaagta atatcttaaa
     2281 acagcccgct ggcagtgttg aacggaaaaa taccattagt gactttacac ctgaagaaaa
     2341 agcgcgttta acatcatgtt caatgtcggt tgttagcctt gattataatg gctatcgctt
     2401 aaacttactt gatgcaccag gcaatgatga ctttgttgct gatgttgttg gtgtgcttga
     2461 ctttgttaaa gcagcagtct tagtcgtcga tactggtaag attgaagtcg gaacagttaa
     2521 acactttaat ttatta
//
LOCUS       DAOSEX010000110         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_40350_length_2512_cov_2.991046, whole genome shotgun sequence.
ACCESSION   DAOSEX010000110 DAOSEX010000000
VERSION     DAOSEX010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40350_length_2512_cov_2.991046"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="queA"
                     /locus_tag="PK612_02640"
     CDS             <1..634
                     /gene="queA"
                     /locus_tag="PK612_02640"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOH95039.1"
                     /translation="EGVFLEVLERVGTTPLPPYIEDKSEKYARYQTVYAKAPGSAAAP
                     TAGFHFTPEILDELKTKGVIVVEITLHIGLGTFKPVSVSDTKDHKMHYETYEISEEAA
                     ATINKAIEEKRRIISVGTTATRTLEANFNKYQKIVATSEATNLFITPGYKFKVISALI
                     TNFHLPKSTLIMLVSAFAGRENTLAAYCHAIKERYRFFSFGDVMYIYGED"
     gene            621..1283
                     /locus_tag="PK612_02645"
     CDS             621..1283
                     /locus_tag="PK612_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOH95040.1"
                     /translation="MEKINYYNISIAEVNNFKDQGLRPKLALHACCAPCSSHTLEFLT
                     PFFAVTIVYQNSNIYPKAEFKKRRDEVERFVREFNIKNNQDVKVVFLPYDHEAFMKEL
                     RVFASEKEGGKRCHLCYEKRIVEALKYGSENGFDYATTTLTISRQKNSQVINAIAKKI
                     APRYPNIKYFYSDFKKKAGLLRVREMKKEYNLYQQEYCGCEYSYRDTVLARNNKKDTE
                     NR"
     gene            1611..1922
                     /gene="rpsJ"
                     /locus_tag="PK612_02650"
     CDS             1611..1922
                     /gene="rpsJ"
                     /locus_tag="PK612_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOH95041.1"
                     /translation="MAGKQKIRVRLQSYDHALLDEAVAKIVEQAVESGAKVVGPIPLP
                     TQREVYTVLRAVHKYKDAREQFEIRTHKRLIDIVDPNKRTVEFLRKFELPSGVDIDIK
                     L"
     gene            1935..>2512
                     /gene="rplC"
                     /locus_tag="PK612_02655"
     CDS             1935..>2512
                     /gene="rplC"
                     /locus_tag="PK612_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOH95042.1"
                     /translation="MKAIIGRKMGMTQVFAEDGTIYPVTVVEVLPNVVTQVKTVEKDG
                     YAAIQVGYQDKKPSRVNKPLKGIYEKAGVTPKYGLYELKGEEMLSYEVGSEIKVDIFK
                     KGDIVDVTGTSKGKGYTGVIKRYGFKLGNAAHGSGYHRGIGSQASVGRIQRVIPGTKM
                     PGHHGAQRTTVLNLLVVDVLPEKNALLIKGAIP"
BASE COUNT          864 a          442 c          527 g          679 t
ORIGIN      
        1 tgagggtgtc tttttagaag tcttagaaag ggttgggaca accccgctcc cgccttatat
       61 tgaagataaa agtgaaaaat atgcgcgtta tcaaacggtg tacgctaaag cgcctggtag
      121 tgcggcagca ccaacagcag gctttcactt tacgccggaa atattagatg agttaaaaac
      181 taaaggagtg atagtcgtcg aaattacact gcatattggc ttaggtactt ttaaaccggt
      241 cagtgttagt gatactaaag atcataagat gcattacgaa acttatgaaa ttagcgaaga
      301 agcggccgca acaataaata aagcgattga agaaaaacga agaatcatca gtgttggcac
      361 aaccgcaaca agaacactag aagctaattt taataaatat caaaagattg tagcgacgag
      421 tgaagcaaca aatttattta ttactccagg ttataaattt aaagtcatca gcgcgttaat
      481 tacgaatttt catttaccta aatctacact aattatgtta gttagtgctt ttgcggggcg
      541 tgaaaataca ctcgcggctt attgccacgc aattaaagaa cgctatcgct ttttctcttt
      601 tggtgatgta atgtatatat atggagaaga ttaattatta caacatttcg attgcagaag
      661 ttaataattt caaagatcaa gggcttagac caaaactagc gctccatgct tgctgtgcgc
      721 cgtgttctag tcacacctta gaatttttaa caccattttt tgctgtgaca attgtgtatc
      781 aaaatagcaa catctatccc aaagctgagt ttaagaaaag acgcgacgaa gttgaaagat
      841 tcgtaagaga atttaatatt aaaaataatc aagatgtcaa agtcgtgttt ttaccatatg
      901 atcacgaagc ctttatgaag gaattaagag tgttcgctag tgaaaaagaa ggcggtaaac
      961 gctgtcatct ttgttatgaa aaacgtattg tagaagcttt aaagtatggt agtgaaaacg
     1021 gatttgatta cgccactacg acgcttacta tctcgcgaca aaagaactca caagtcatca
     1081 atgcaattgc taaaaagatt gcgcctcgct acccaaatat aaaatatttt tatagtgatt
     1141 ttaagaaaaa ggccggatta ttgcgtgtcc gtgaaatgaa aaaagaatat aacctttatc
     1201 agcaagaata ttgtggttgt gaatactctt atcgcgacac cgttttagca agaaataata
     1261 aaaaagacac cgaaaaccgt tgatttttca cgatttttgg acaaaaacag cagtatttaa
     1321 gaaaaaatgg aaccgcttgc aaaagtggtt tttttcacga ttttcggtaa aaagttcaaa
     1381 agtttgaaat ttggtttttc acaagatttt gtgaccttat atataaaaat atatattttt
     1441 tatcgtttat ggttgacatt atattatgct tttagtatta tttagcaagt taatggtcgc
     1501 gttgaaatgc gaggttgccg atacacctac aagcgttgtc atgaagtgta atcggggaat
     1561 tcgtagctga ggaccattag cgcctgtaga tgtaaggagg aaaataattc atggcaggaa
     1621 aacaaaagat aagggttcga cttcaaagtt atgatcatgc attacttgat gaagcagtag
     1681 cgaagatcgt tgaacaagca gtcgaaagtg gcgccaaggt agttggcccc atcccgctcc
     1741 caacacaacg tgaggtgtac acggtgttac gagcggtcca caagtacaaa gatgcgcgtg
     1801 agcaattcga aattagaaca cacaaacggc ttatcgatat cgtcgatcca aacaaacgca
     1861 cagtggaatt cttacgaaag tttgaactcc caagtggtgt cgacatcgat attaaactat
     1921 aaggaggtag aaatatgaaa gcaattattg gccgcaaaat gggtatgacc caagtattcg
     1981 ccgaagatgg cacaatctac cccgttacag ttgttgaagt cttaccaaat gttgtcactc
     2041 aagtgaaaac agtggaaaaa gatggctatg ccgccatcca agtaggctat caagataaaa
     2101 aaccgagccg ggtaaataaa ccacttaaag gcatatacga aaaagcaggt gtcactccaa
     2161 aatacggttt atacgaatta aagggtgaag aaatgcttag ctacgaagtt ggtagcgaaa
     2221 ttaaagtcga catcttcaaa aaaggagata tcgtcgacgt aactggaaca agcaaaggga
     2281 aaggctacac tggtgtcatt aaacgctatg gcttcaaact tggaaatgcc gcccacggtt
     2341 ctggttatca ccgcggaatt ggttcgcaag ctagcgttgg tcgtattcaa cgcgtaattc
     2401 ccggaacaaa aatgcctgga catcatggag cgcaaagaac aacagtctta aacttactcg
     2461 ttgttgatgt cttaccagaa aagaatgcac tcttaattaa aggtgccatt cc
//
LOCUS       DAOSEX010000111         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_40499_length_2506_cov_3.864137, whole genome shotgun sequence.
ACCESSION   DAOSEX010000111 DAOSEX010000000
VERSION     DAOSEX010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40499_length_2506_cov_3.864137"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="PK612_02660"
     CDS             <1..1272
                     /locus_tag="PK612_02660"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HOH95043.1"
                     /translation="SLLAAVAEPGITVLAAQVGGIHPRINSTLFIWVTSFGMGIFTAL
                     AVYRLLKGVSMKKIFFFFYLAVIIIIFFVPSQFQALGFDASGAATGAISVPFILALGV
                     GVSHTVSRNKSAEDSFGIVGIAAIGPIITVYLYGLIVGNSDTLNVYTPGENSDLLAVM
                     LGNLLPVALAVFPLVLIFLIFQAFFIKLPRRKIGQIIIASLVVFVGLFIFIAATDFGL
                     AFAGKYIGKQFMMSEKLWWLLLPLAFVLGFAITLSEPSVVVFCEQVDELTSGSISKNT
                     IKMALGISVGLATFFAVIKTILDINILWFIVPFYVITIGLIYFTPTLFVGLAFDGGTV
                     SAGAITSAFLTPVTLGASQALGHTNILTSGLGILAFISVMPRIVVQILGIIYNYRLKK
                     ATPPVYEDISYDATTLSETETEIQEEATNDA"
     gene            1262..1570
                     /locus_tag="PK612_02665"
     CDS             1262..1570
                     /locus_tag="PK612_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95044.1"
                     /translation="MTHKEEWVVMVIITRRKYKAELVKMLYDHEAAMVHQTYAKGSAK
                     ASFLRALGLTQEQSKLFLLGFVKTDQVPAIFSVLEYKYKFKEPNSGFAFTFPLEKISY
                     "
     gene            1583..1891
                     /locus_tag="PK612_02670"
     CDS             1583..1891
                     /locus_tag="PK612_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOH95045.1"
                     /translation="MTENMKALFIIVNAGFSEQIVEVVQNHGARGATIIPARGTGKKF
                     VKVLGIQYEPEREILLSVVEEDIALKITADVKENYGKNTPTNGLCFILPVADYMFKNE
                     "
     gene            1943..>2506
                     /locus_tag="PK612_02675"
     CDS             1943..>2506
                     /locus_tag="PK612_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95046.1"
                     /translation="MGNFIRKHIDTLLLVLNVLVFVLFTLVYPFVTFKHTFLNALFYI
                     LLSAPLITFLVLRFLKYDRKTEESIFYYRRGFNLLALINNYLFMMSAESIFTLIKGHR
                     APLFIIYLIFFALMWLINIFALILELGYLDQVLPLREYATDTAKEETIALAKAAKFKR
                     FEEKVSTMYFFIALVAVLAVIFFIYPLV"
BASE COUNT          717 a          410 c          499 g          880 t
ORIGIN      
        1 agtttactcg ctgctgtggc ggaaccaggc attactgttt tagctgcaca agttggtggt
       61 attcacccac gtataaatag cacactcttt atttgggtaa ctagttttgg tatgggaata
      121 tttacggcct tagcggtata tcgtttgtta aaaggtgtaa gtatgaaaaa aatcttcttt
      181 ttcttttatt tagcagttat cattattatt ttctttgttc caagtcaatt ccaagccctt
      241 ggctttgacg ctagtggggc ggcaacaggt gcgattagtg ttccctttat tttagcgtta
      301 ggtgtaggtg tttcccatac tgttagtcgc aataaaagtg cagaagatag ttttgggatt
      361 gttggtattg cggcaattgg tccaattatt actgtttact tatatggatt aatcgttggt
      421 aatagtgaca cacttaatgt atatactccc ggtgaaaata gtgatttact agcggtaatg
      481 cttgggaatt tattgccagt cgcccttgcg gtctttccgc ttgtgttaat ttttcttatt
      541 ttccaagcat tctttattaa gttgccgcga agaaaaattg gtcaaataat aattgcatca
      601 cttgttgttt ttgttgggtt atttattttt attgcagcga cagactttgg gttagcgttt
      661 gctggtaaat atattggcaa acaatttatg atgagtgaaa aactgtggtg gctactttta
      721 ccactagcgt ttgtacttgg ctttgcaatt actttaagcg aaccaagtgt tgttgtattc
      781 tgtgagcagg ttgatgaatt aactagcgga agcatttcta aaaatacaat taaaatggct
      841 ttaggtattt ccgttggctt agcgacattc tttgcagtta ttaaaacaat tttagatatt
      901 aatatcttat ggtttatcgt acctttttat gtaattacaa ttggtttaat ttactttaca
      961 ccaacattat tcgttggttt agcctttgat ggcggtacgg taagtgcggg cgcaattacg
     1021 agtgcctttt taaccccggt gacactcggg gctagtcaag ctttagggca cactaatata
     1081 ttaacctcag gcttaggtat tttagccttt atcagtgtga tgccgcgtat cgttgtgcaa
     1141 atacttggca taatttataa ctatcgtttg aagaaagcaa caccaccagt gtatgaagat
     1201 attagttatg atgcgacaac gttaagcgaa acagaaacag aaatacaaga ggaggccaca
     1261 aatgacgcat aaagaagaat gggttgtgat ggttattatt actcgtcgca aatataaagc
     1321 ggagctcgta aaaatgctct atgaccatga agcagcaatg gttcatcaaa catatgcgaa
     1381 ggggagcgct aaagctagtt tcttacgggc acttggccta actcaagaac aaagtaagct
     1441 ctttttgcta ggatttgtca agactgatca agttccagca atctttagtg tattagagta
     1501 taaatacaaa tttaaagaac caaattctgg gtttgctttt acattcccac ttgaaaagat
     1561 aagttactag gaggacattc ttatgactga aaatatgaaa gctttattta ttatagttaa
     1621 cgcgggcttt agtgaacaaa tcgttgaagt cgtgcaaaat catggcgcta gaggtgctac
     1681 tattatcccc gcgcgtggca ctggcaagaa gtttgtcaaa gtacttggta ttcaatatga
     1741 accagagcgc gaaatcttac ttagtgtcgt ggaagaagat atcgcgctta agattactgc
     1801 tgatgtgaaa gaaaactacg gtaaaaacac accaactaat gggttatgct ttattttgcc
     1861 tgttgctgat tacatgttta aaaatgagta aataataatt tgataactat taatcttcaa
     1921 tttattttat aatgataata ctatgggaaa ctttattcgt aaacacattg atactttact
     1981 cctagtttta aatgtcttag tctttgtttt atttacgctt gtttacccat tcgtgacctt
     2041 caaacatacc ttcttaaatg cactattcta tattttactt agtgcaccgc ttattacttt
     2101 cttagtgctt aggttcctta aatatgaccg caagactgaa gaaagtattt tttattacag
     2161 gcgtggcttc aaccttttag cgcttattaa taactattta tttatgatga gtgctgagag
     2221 tatttttacg cttattaaag gtcatagagc gccgttattt attatttatt taatattctt
     2281 cgctttaatg tggttaatta atatctttgc actaatcctt gaacttggtt acttagatca
     2341 agttttaccg cttcgagaat atgcgactga caccgcgaaa gaagaaacga tcgcattagc
     2401 gaaagctgca aagtttaaac gctttgaaga aaaagtttca acaatgtact tctttattgc
     2461 gcttgtggca gtactcgcgg ttattttctt tatttatcca ctggtg
//
LOCUS       DAOSEX010000112         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_40597_length_2502_cov_3.110339, whole genome shotgun sequence.
ACCESSION   DAOSEX010000112 DAOSEX010000000
VERSION     DAOSEX010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40597_length_2502_cov_3.110339"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PK612_02680"
     CDS             <1..181
                     /locus_tag="PK612_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HOH95047.1"
                     /translation="ALTVEEVYKVFNGDIAGILEGETTSHIPSTIVKVDDKIELIREG
                     SIPFNLIKEVWEEEL"
     gene            178..621
                     /gene="rpiB"
                     /locus_tag="PK612_02685"
     CDS             178..621
                     /gene="rpiB"
                     /locus_tag="PK612_02685"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009619525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOH95048.1"
                     /translation="MKVAIGSDHGGFIYKEAVKKHLEDKGITVIDVGSYHEDSVDYPL
                     YGIAVGEKVRDKSADLGIVICTSGEGITIAANKVKGVRAGIGYNDDVSRLIREHNNAN
                     VIGFGGSQMELKDVLRRVDIFLNARFESGGRHSRRVGIITEYENK"
     gene            662..1831
                     /locus_tag="PK612_02690"
     CDS             662..1831
                     /locus_tag="PK612_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOH95049.1"
                     /translation="MQDFFICPTCGNNDLNKIGVRNGQKYCRACLPFQGQNAPKITNI
                     PLHIPITLSYELTTEQRRIAEEINANYINKQNSLVYAVCGAGKTELVYLAMQTALARG
                     HQIGFTVPRREVVIELAMRIKKTFPQARVISVFGDHHDALTGNIIVLTTHQLFRYEDY
                     FDLLIFEEIDAFPYVGDLVLESFFKRSVKGVYVLLSATPSTKLLKEFPKVGKVLTLFT
                     RFHNHKIPVPIVKRGFLLFNFMLLLKHLESFIKRKKPVLIFVPTIRLANLIYKLLRID
                     YKNGNVVHSKVKDNSAIIDDFKKGHYAYLVTTTVLERGVTIKGLQVIIYGADHAVYDT
                     ASLVQISGRVGRKKEESDGEVIFIVTKKNAAIKAAISDIEEKNAYLQSVLQRKAT"
     gene            1806..2396
                     /locus_tag="PK612_02695"
     CDS             1806..2396
                     /locus_tag="PK612_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOH95050.1"
                     /translation="MCFKEKQPNFNDLIRDTNVCNKCFRDLDVKFNVSKIAGVTYISV
                     FNYDEHFKTLLFHLKGLKDIVLAPLFLERFIPYFKVRFINYYLVPAPSTIESDKERGF
                     NHVIEIFKPLQRPFLRLITKKVAFKQSDLNYALRQKVKDKLVINNGNKVKGKNILIVD
                     DIKTTGATIMAMINLIKPYNPRKIAILTLAETSLSE"
BASE COUNT          888 a          358 c          476 g          780 t
ORIGIN      
        1 tgctttaaca gttgaagaag tatataaagt ctttaatggt gatattgccg gcattcttga
       61 aggcgagacg acatcgcata ttccgtccac aatcgttaag gtggatgata aaatagaatt
      121 aattagagaa ggaagcattc cctttaattt aattaaagaa gtttgggagg aagaactatg
      181 aaagtagcaa ttggcagtga tcatggtggc tttatttata aagaagcagt aaaaaaacat
      241 ttagaagata aagggattac cgtaattgat gtcggttcat atcatgaaga tagtgtagac
      301 tatccgcttt atgggatagc tgttggtgaa aaagttcgtg ataaatctgc tgatttagga
      361 atagttattt gtacatcagg cgaaggcatt actattgccg caaataaggt taaaggggta
      421 cgcgctggta ttggttataa tgatgatgtt tcgcgtctca ttcgtgaaca taataatgcc
      481 aatgtaattg gttttggtgg ttcacaaatg gaattaaagg atgttttacg ccgtgtcgat
      541 atttttctta atgctagatt tgaaagtggt ggtcggcatt cgcggcgtgt cggtatcatc
      601 actgagtacg aaaataaata attactgata aataaagggt cttgcttaca tacttataaa
      661 tatgcaagac ttttttattt gcccgacttg cgggaataac gatcttaata aaataggagt
      721 aagaaacggc caaaaatatt gccgagcatg tttacccttc caagggcaaa acgcgcccaa
      781 aataactaat attccactgc atataccaat aacactaagt tatgaattaa caacagaaca
      841 aaggcgaatt gccgaagaaa tcaatgcaaa ttacataaat aaacaaaatt cattagtgta
      901 cgctgtatgt ggcgctggta aaactgaatt agtgtattta gcaatgcaaa ctgcactagc
      961 gcgtggccat caaataggtt ttacagttcc gcggcgtgaa gtcgtaattg agttagcgat
     1021 gcgcattaaa aaaacttttc cgcaagcgag agtaatcagt gtttttggtg accatcacga
     1081 tgctttaact ggcaatatta ttgtacttac tactcatcaa ctttttagat atgaagatta
     1141 ttttgattta ttaatttttg aagaaattga tgcttttcct tatgttggcg acttagtgct
     1201 tgaatcattt tttaaacgca gtgtcaaggg tgtatatgtt ttactaagtg ctactccgag
     1261 tacaaagcta ttaaaagagt ttccaaaagt tggtaaagtg ttaactcttt ttacaagatt
     1321 tcataatcat aaaatcccag tgccaatagt gaaacgcggt tttttactat ttaattttat
     1381 gttgttatta aaacatttag aatctttcat taaaagaaag aaaccagttt taatttttgt
     1441 accaacgata agactcgcta atttaatata caaactatta cgcatcgatt ataaaaatgg
     1501 taatgtagtg cattcaaaag taaaagataa ttcagcgata attgatgatt tcaaaaaagg
     1561 acattacgcc tacttagtaa cgacaacagt cttagaaaga ggggtaacga ttaaagggtt
     1621 acaagtaata atttatggcg cagatcacgc agtgtacgac actgctagtt tagtgcaaat
     1681 ttctggaaga gtcggacgaa agaaggagga gagtgatggt gaagttattt tcatcgttac
     1741 gaaaaagaat gcggcaatca aagcagcaat tagtgatatt gaagaaaaaa acgcctattt
     1801 gcaaagtgtg cttcaaagaa aagcaaccta actttaatga tttgattcgt gacactaatg
     1861 tttgtaataa atgttttcgt gatttagatg ttaagtttaa tgtttcaaag attgcaggag
     1921 ttacttatat tagtgttttt aattatgacg aacattttaa aacactactt ttccatctaa
     1981 aaggtttaaa agacattgtg ctagcgccac tttttcttga aaggtttatt ccttacttta
     2041 aagtacggtt tattaattac tatttagtgc cagcgccttc aacaatagag agtgataaag
     2101 aacgcggatt taatcatgtc atcgaaatat ttaaaccgct acaaagacct tttttaaggc
     2161 ttattacaaa gaaagttgcc ttcaaacaaa gtgacttaaa ctatgcactc cggcaaaaag
     2221 taaaagataa attagtaatt aataacggaa ataaagtaaa aggtaaaaat attttaatcg
     2281 ttgatgatat taaaacaaca ggtgccacaa ttatggcaat gattaactta ataaaaccct
     2341 ataatccgcg gaaaattgca atattaacac tagcggaaac atcgctaagc gagtaactat
     2401 catattttta tagaaaatat ggtaaacttt tatagataca tgatacgaag gagattaact
     2461 attcttaatc aagttaaata gtaaatatat atcaaaaaga tt
//
LOCUS       DAOSEX010000113         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_41070_length_2482_cov_3.892872, whole genome shotgun sequence.
ACCESSION   DAOSEX010000113 DAOSEX010000000
VERSION     DAOSEX010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41070_length_2482_cov_3.892872"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="rplF"
                     /locus_tag="PK612_02700"
     CDS             complement(<1..305)
                     /gene="rplF"
                     /locus_tag="PK612_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOH95051.1"
                     /translation="MSRIGLKVIKVPAGVEVKLEGDLITVKGPKGTNHVTLPLHITLD
                     QSHEGEIHVKRDNELKQTKQSHGTTRALLHNAIVGAHEGFTIRLQIVGIGYRAIQEG"
     gene            complement(316..711)
                     /gene="rpsH"
                     /locus_tag="PK612_02705"
     CDS             complement(316..711)
                     /gene="rpsH"
                     /locus_tag="PK612_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOH95052.1"
                     /translation="MSMTDPIADMLTRIRNANALSHEAVAMPSSKNKVNIARILKEEG
                     YITDYFVSGDVKKVLTLVLKYGPNGEKVISGLKRISKPGLRVTTTSDKLPRVLRGLGI
                     AIISTSQGIMTDKKARALGVGGEVVAYVW"
     gene            complement(727..912)
                     /locus_tag="PK612_02710"
     CDS             complement(727..912)
                     /locus_tag="PK612_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004268593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOH95053.1"
                     /translation="MAKTSKRVAQLRKPKFKVRAYSRCSRCGRPHSVYRKFGVCRICL
                     RELAYNGEIPGMKKASW"
     gene            complement(927..1478)
                     /gene="rplE"
                     /locus_tag="PK612_02715"
     CDS             complement(927..1478)
                     /gene="rplE"
                     /locus_tag="PK612_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010580223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOH95054.1"
                     /translation="MKKSTNRVVRQYEDVAIKALMEKFNYTSVMQVPKLSKIVINMGV
                     GDATQNAKLLEDAVRELTVIAGQKPVVTIAKKSEASFKLRAGTAIGCKVDLRGERMYD
                     FFEKLISIALPRVRDFRGVSKDSFDGHGNYTLGVREQLIFAEIDFDLVNKVRGMDIVI
                     VTTAKTDAEAYELLKLLGMPFRR"
     gene            complement(1792..2100)
                     /gene="rplX"
                     /locus_tag="PK612_02720"
     CDS             complement(1792..2100)
                     /gene="rplX"
                     /locus_tag="PK612_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012656117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOH95055.1"
                     /translation="MLIKKGDKVIVITGEDKGKTGIVQRAYPKLQKVVVEGVNVRKKH
                     KKPTQQNPEGSILDVYAPIHVSNVALIDPKTKKPTRVGMKEVKGKKVRYAKKSGEVIK
                     "
     gene            complement(2111..2482)
                     /gene="rplN"
                     /locus_tag="PK612_02725"
     CDS             complement(2111..2482)
                     /gene="rplN"
                     /locus_tag="PK612_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOH95056.1"
                     /translation="MHVETKVNIADNSGARIGKVIRLYGGSVRRHSNIGDIVLITVRQ
                     AVPNSKVKKGDMVKAIVVRTKKGVLRPDGSKIKFDDNAVVIINNDGNMIGTRVFGPVA
                     RELREKGDGAMKIVSLAPEVL"
BASE COUNT          571 a          576 c          463 g          872 t
ORIGIN      
        1 tcgccttctt gaatagcgcg gtaaccgata ccgacgattt gtaagcgaat agtaaaacct
       61 tcatgcgctc caacaattgc attgtggagt aaagcacggg ttgtaccatg actttgtttt
      121 gtttgtttta attcattgtc gcgtttaaca tgaatttcac cttcatgact ttgatcaagg
      181 gtgatatgga gtggtaaggt aacgtgattt gtcccttttg gtcctttaac ggtgattaag
      241 tcaccttcaa gtttcacttc tacgcctgct ggcactttaa ttacttttaa accaattctt
      301 gacataactt acctcctacc acacataggc gactacttcg ccaccgacgc ctaaggcgcg
      361 cgctttcttg tcagtcatga ttccttgtga tgtggaaata atagcgatac ctaaaccgcg
      421 taaaacgcgt ggtaatttat cgctggtagt ggtgactctt aagcctggct tagaaatacg
      481 ttttaaacca gaaattactt tttcaccatt tggtccgtat ttaagcacta aggttaaaac
      541 ttttttaacg tcgccactaa cgaagtaatc agtaatataa ccttcttctt ttaagatacg
      601 ggcaatatta actttgtttt tgctgctcgg catcgcaact gcttcgtgac ttaaagcatt
      661 agcgttccga atgcgggtta gcatatctgc aattggatct gtcatactca tactagttgc
      721 tcctccttac cagctggcct tcttcatgcc aggaatttcg ccattataag cgagttcacg
      781 taaacaaata cggcacacac cgaatttgcg ataaacgcta tgaggtctgc cacaacgtga
      841 acaacgacta taggcgcgta ctttaaattt aggtttgcgt aattgcgcta ctcttttact
      901 tgtttttgcc ataatctctc cctcctctag cgtctaaacg gcataccgag tagtttaagc
      961 aattcatatg cttcggcatc cgttttagcg gttgtgacaa taacgatgtc catcccgcgg
     1021 actttattta ctaagtcaaa gtcaatttca gcgaagatta gttgttcgcg gactccgagt
     1081 gtatagttcc cgtgaccgtc gaagctatct ttactgacac cgcggaagtc acgcacacgt
     1141 gggagcgcga tactaattaa cttctcgaag aagtcataca ttctttcgcc gcgtaaatca
     1201 actttacaac caattgcggt tccagcacga agtttgaagc ttgcttcact tttcttcgca
     1261 attgtcacca ctggtttttg accggcaatg acggttaatt cccgcactgc gtcttctaat
     1321 aatttagcgt tttgtgttgc atccccaacc cccatattaa tgacaatctt gcttaatttt
     1381 gggacttgca tcacgcttgt gtagttaaac ttttccatta aagctttaat cgctacatct
     1441 tcatattgac ggacaacacg atttgtgctc tttttcactt catggacata acgttcaact
     1501 ttatgttctt tttcaacctt tttggctggt ttttctttag caacttccgc tttagcttcg
     1561 gctttcagcg cagctttttt cgtttcagct tttgctgctt ctttttttgg cgctgctttt
     1621 tttacttcag ctttcgcggt ttctttcttt tcagtagttt tcgcttctgc ttttttagga
     1681 gcagcggctt tcttttctac ttttttagtt tcggctttag gagctgcttt ttttggagca
     1741 tcttcttttg caacagtttt ctttgcttta tcttctgcca taatgcacct cctatttaat
     1801 tacttcgccc gactttttag cgtagcgaac ttttttgcct ttcacttctt tcatcccaac
     1861 gcgtgttggt ttttttgttt ttggatcaat aagtgcgaca ttgctcacat gaatcggcgc
     1921 atacacatct aagattgatc cttcagggtt ttgttgggtt ggttttttat gtttcttgcg
     1981 aacattaacg ccttccacaa caaccttttg taatttcgga tacgcccgtt ggacaattcc
     2041 ggttttgcct ttgtcttcgc cagtgatgac gatgacttta tcaccttttt taattaacat
     2101 acgagccctc ctataatact tctggagcaa ggctaacaat tttcattgcg ccatctccct
     2161 tttcacgtaa ttcacgagct acggggccaa acacccgtgt cccgatcatg ttgccgtcat
     2221 tattaataat aacaacagcg ttatcatcaa atttaatttt tgatccgtct ggccgtaaca
     2281 cacctttttt agtgcggacg acaatcgctt tcaccatgtc acctttttta actttggagt
     2341 tagggacggc ttgtctgact gtaattagga cgatgtcgcc aatgttactg tgccgacgga
     2401 cactgccacc atagagtcta ataacttttc cgattcgggc accggagtta tcagcgatgt
     2461 ttactttagt ttcaacgtga at
//
LOCUS       DAOSEX010000114         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_41464_length_2466_cov_5.682704, whole genome shotgun sequence.
ACCESSION   DAOSEX010000114 DAOSEX010000000
VERSION     DAOSEX010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41464_length_2466_cov_5.682704"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK612_02730"
     CDS             complement(<1..232)
                     /locus_tag="PK612_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95057.1"
                     /translation="MKILDCFSGNLSTWFDKDFAKNNVVKNDIRKGLTLEKQGQIIEI
                     VADYNNNMFAENTIIDGGEFDMIYADPPHLVTT"
     gene            complement(253..495)
                     /locus_tag="PK612_02735"
     CDS             complement(253..495)
                     /locus_tag="PK612_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95058.1"
                     /translation="MKYRKKPVVIEAVQFNGRNSADIHEFCGDKVREPVGKDYLEIKT
                     LEGIHIASPGDYIIKGVNGEFYPCKPDIFDKTYEKV"
     gene            complement(576..947)
                     /locus_tag="PK612_02740"
     CDS             complement(576..947)
                     /locus_tag="PK612_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95059.1"
                     /translation="MKLKDRLNSENRAIWNTLQYRYRNYIETEMMCRGDMSNDELNVQ
                     IKPLIMHYQKKYAPFKPKIYIKKKSAASKIARIRLYIDELKESLNESSVLDGKYAIYE
                     QGAKDATKHTIERLLKIIGDE"
     gene            complement(951..1388)
                     /locus_tag="PK612_02745"
     CDS             complement(951..1388)
                     /locus_tag="PK612_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95060.1"
                     /translation="MSELTSRQWRFYNYLKEQALLNQNRWVGKEEIHFALLGDYKLNN
                     HAKSHDICAAMNSDMMAINRSEVIEKLIILKNNEFKIATEQEAREYIEKHKKQIENHA
                     KRINKLVYKLRKNGQYKLLSNSNVPMDQSQGREYVETFIERYN"
     gene            complement(1401..1652)
                     /locus_tag="PK612_02750"
     CDS             complement(1401..1652)
                     /locus_tag="PK612_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95061.1"
                     /translation="MTISRIEEIIEKRFQIKMTRNRSYQILWHSGDERILVSNIRSDY
                     MIIEIEKGLATLKSPFANERQLIQILHYFVNIAGELNKQ"
     gene            complement(1688..2146)
                     /locus_tag="PK612_02755"
     CDS             complement(1688..2146)
                     /locus_tag="PK612_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95062.1"
                     /translation="MEKKKQPLTLKALNEKIEELLDKYGEDDSVAYYYDANKKTIIKG
                     KLVLPHKEPQELKYERLLFIGGQDEYDFYYIKLGIRQFFSNGGKVACVGFNPEKNEFF
                     GWSHRGYGRFYVGYVVKEKSMLSEQIAAGYVTKTLQHCKVLAHIFADLMD"
BASE COUNT          641 a          472 c          308 g         1045 t
ORIGIN      
        1 ttgtggttac taaatgcggc ggatcggcgt aaatcatatc gaactctccc ccatcaataa
       61 ttgtgttttc ggcaaacatg ttgttgttgt aatcggcaac tatttcaata atttgacctt
      121 gcttttcaag tgttaaccct ttacgaatgt cattttttac gacattgttt tttgcaaaat
      181 ctttatcaaa ccaagttgat aaattaccac tgaaacaatc taatattttc atactatctc
      241 ctttacactt tcttaaactt tctcataagt tttatcaaag atatcaggtt tacaagggta
      301 gaattctcca ttcacacctt taattatgta atcgccggga cttgcaatat gaataccctc
      361 taatgtcttt atttcaagat aatctttacc aactggttct cgtactttat caccacaaaa
      421 ttcgtgaata tctgctgaat ttcttccatt gaattggact gcttcaatta caactggttt
      481 ctttctgtat ttcatatcat ctttctcact ttcttaaact ttttaattaa gtctaataaa
      541 tcttgttcag ttcctataaa tgtataacta ttcatttatt catctcctat aattttcagt
      601 aatcgctcaa tcgtgtgttt tgtcgcatct tttgcccctt gctcataaat cgcatattta
      661 ccatctaaca cgctgctttc atttaatgat tcttttaatt catcaatata tagtcttatt
      721 ctcgctattt tagatgccgc actctttttc ttaatatata tttttggctt aaatggtgca
      781 tatttttttt gatagtgcat aataagtggt ttgatttgaa catttaattc atcatttgac
      841 atatcaccac gacacatcat ttccgtttct atataattgc ggtatcggta ttgaagcgtg
      901 ttccaaatcg ccctattttc actatttaat ctatctttta gtttcatatt ttaattatac
      961 ctctcgataa atgtctcgac atattcacga ccttgacttt gatccattgg cacattacta
     1021 ttcgataaca gcttgtattg accgtttttt cttaacttat acactaactt attgatccgt
     1081 tttgcgtgat tctcaatttg ttttttatgc ttttcgatat attcccttgc ttcttgttcg
     1141 gtcgcaatct tgaactcatt gttttttaag atgattagtt tttcgatgac ttcacttcga
     1201 ttaatcgcca tcatatcgct gttcattgct gcgcatatat cgtgcgattt agcgtgatta
     1261 ttaagtttat aatctcctaa taaggcgaag tgtatttctt ccttgcccac ccacctattt
     1321 tgatttaata aggcttgttc ttttagataa ttgtagaatc gccattgtct gcttgtcagt
     1381 tcactcattt tctttctcct ttattgttta tttaattcgc cagcaatatt cacgaaatag
     1441 tgtaagattt gaattaattg cctttcatta gcgaatggcg attttagcgt tgccaatcct
     1501 ttttcgattt ctattatcat ataatcactt cgtatatttg atactaatat tctctcatcg
     1561 ccactgtgcc ataagatttg ataactccta tttcttgtca tcttgatttg aaatcgtttt
     1621 tcgattattt cttcaattct tgatattgtc atttagttat tctccttttt tatattttta
     1681 taaactctta atccattaaa tcggcaaata tatgtgctaa aactttacaa tgttgcagcg
     1741 tttttgtaac ataaccagcg gcgatttgtt cggacaacat tgatttttcc ttaacaacgt
     1801 aaccaacgta aaaccttcca taaccccgat gcgaccaacc aaaaaactcg tttttttcgg
     1861 gattgaaacc aacgcacgct acttttccac cattactgaa aaactgtcta attcctaact
     1921 ttatatagta aaaatcatat tcatcttgcc ctccgataaa aagtaatctt tcgtatttta
     1981 attcttgtgg ttctttgtgt ggtaacacta attttccttt aatgattgtt tttttgttag
     2041 cgtcataata atatgccaca ctatcatctt cgccatattt atctaataat tcttcgattt
     2101 tttcgtttaa cgctttaagc gttagtggtt gtttcttctt ttccatttaa tcattcctcc
     2161 tatttcttgt catcttgatt tgaaatcgtt tttcgattat ttcttcaatt cttgatattg
     2221 tcatcttaac ccctttgctt tttttattat ttttattaga tttgataaat ctttttcggc
     2281 tctaatcgtt ttagatattt taatgtctat atcttttttt gttagattgt ataccacgaa
     2341 cactccttgg cgatattccc ttaaataaac ctcgtaattt ttagaaatat agtagtcact
     2401 attaggatat aatttgatat taagttcgtt ttgtatcact tcgataatgt cggttgtttt
     2461 cattta
//
LOCUS       DAOSEX010000115         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_41961_length_2446_cov_3.572146, whole genome shotgun sequence.
ACCESSION   DAOSEX010000115 DAOSEX010000000
VERSION     DAOSEX010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41961_length_2446_cov_3.572146"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PK612_02760"
     CDS             complement(<1..840)
                     /locus_tag="PK612_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOH95063.1"
                     /translation="MKWVFLGLAILIFLVISLYFWTVMITFRMLFRRPKLPKKLVFPK
                     AFFGGVFDKHIPEIEANHKKNAKKACEIVTISSSAGKLKGRFYENENSNKIVIFIHGY
                     YSSGMRDIGYFGSLHKELNVSLLVIDQRANGESDGVYTSFGALERYDIREWIFYLNAR
                     YKGEKDIYLHGISMGAATSLLVTALAALPPSFKGVIADCGFSRTNGVIFFAGKRMLKI
                     KPKLTFWGLNLIAKLAGGFDLNKVNVSRELHKNTKIPILFIHGTADLFVPYNMSVKNY
                     KATS"
     gene            complement(908..2116)
                     /locus_tag="PK612_02765"
     CDS             complement(908..2116)
                     /locus_tag="PK612_02765"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOH95064.1"
                     /translation="MKKSLVYFQSGGPTAIINTSMYGVIKEAKRQTEYIDGIYGSLHG
                     IEGLLNDNLIDLRQEDEAQIELLKQTPAAALGTTRYKLSGDINHEDYQKILSTVKKHN
                     IGYMLVNGGNDSMDTCYKLSTFFRKMDFDCKVIGIPKTIDNDLAFTDHCLGYPSAARY
                     VVQSIQDIALDNKVYPKSKFVIVEVMGRHAGWLTAAASVIQDPELKPDYIYLPEEKFD
                     LEEFLARAKEVYERKKRALFVVSEGIETQMPNLDKSVDAFGHAQLGGFAAKLAGIVEE
                     RLGYSTRPIEFSLLQRAGTLAITETDQKEAIKVSTVAVKRIVAGKTGQMVIIDRKPGP
                     KYKVSYKLKNLRGIANVEHKMTPELIHDVNNGGKLFGEYLFPLIDKPIKLQLENGVAK
                     YTKLKKVKVK"
     gene            complement(2161..>2446)
                     /locus_tag="PK612_02770"
     CDS             complement(2161..>2446)
                     /locus_tag="PK612_02770"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058008.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOH95065.1"
                     /translation="KKSYGITGKEAEALLETIKITTNKNMLPKDDERPNTTSGLRLGF
                     AAVTTRGLTKVNAAETARIIHEFLSKKITKEEAMIRVNKIVDTLQAIDSL"
BASE COUNT          729 a          474 c          397 g          846 t
ORIGIN      
        1 acttgttgcc ttataatttt taactgacat attataagga acaaataaat cagcggtacc
       61 atgaataaat aaaattggta tcttcgtgtt tttatgtaat tcgcgactta cattaacttt
      121 attaagatca aagccgcccg ctaatttagc tattaggttg agtccccaaa aagtaagttt
      181 cggtttaatc ttaagcatgc gcttaccggc aaaaaagatt acaccattag tgcgcgaaaa
      241 tccgcaatca gcgataacac ctttaaagct cggtggtaat gccgctagtg ctgtaacgag
      301 taagcttgtc gcagccccca ttgaaattcc gtgaagataa atatcttttt cgcctttata
      361 acgagcattt aaataaaaga tccattccct aatatcataa cgctcaagcg ccccaaatga
      421 tgtataaaca ccatcactct ccccgttagc gcgttggtca atgacaagta gtgacacatt
      481 taactcttta tgtaatgaac caaaataacc tatatcacgc attccagaag aataataacc
      541 gtgaataaaa ataactatct tattactatt ctcattttca tagaatcgtc cttttaattt
      601 acccgcactt gaactaatag ttactatttc acatgccttt ttagcatttt tcttatggtt
      661 agcctctatt tccggaatat gtttatcaaa aacaccacca aaaaaagctt ttgggaacac
      721 tagtttctta ggaagttttg gccgacgaaa gagcatccgg aatgtaatca tgacagtcca
      781 aaaatacagt gatattacga gaaatattaa gattgctagc cctaaaaata cccatttcat
      841 acatttatta taacttttaa taaagaaaaa gcggagttta tactccgctc actatttttt
      901 ttactaatta tttaacttta acttttttaa gtttagtgta tttagcgaca ccattttcta
      961 attgaagttt gattggttta tcgattagtg ggaataaata ttcaccaaat aacttaccgc
     1021 cattattaac atcgtgaatg agttctggtg tcattttatg ttcaacatta gcaataccgc
     1081 gtaagttttt aagtttgtaa cttactttgt attttggtcc tggtttacgg tcaataataa
     1141 ccatttgtcc tgttttacca gcaacaatgc gtttgaccgc aacagttgaa actttaattg
     1201 cttctttttg gtctgtttcg gtaattgcta aagtacctgc tctttgtaaa agactaaatt
     1261 caattgggcg cgttgaatag cctaagcgtt cttcaacgat acctgctaat ttggcagcaa
     1321 agccaccaag ttgagcatga ccaaatgcgt cgactgattt atcaaggtta ggcatttgtg
     1381 tttcaatacc ttcactaacg acaaataatg cccgtttttt gcgttcataa acttctttag
     1441 cgcgcgctaa gaattcttct aagtcaaatt tttcttcagg taaataaata tagtctggtt
     1501 taagttctgg atcttggata acacttgcgg ccgcagttaa ccatccagcg tggcgcccca
     1561 tcacttcgac gataacgaat tttgattttg gatacacttt attatctaaa gcgatgtctt
     1621 gaattgattg gacaacataa cgggctgcac ttggataacc taagcagtga tcagtaaacg
     1681 ctaagtcgtt gtcaattgtc tttggaatac cgattacttt acaatcgaag tccattttac
     1741 ggaagaaagt tgataatttg taacaagtat ccattgaatc gttaccacca ttaactaaca
     1801 tgtaaccaat gttatgtttt ttaacggtgc ttaatatttt ttggtaatct tcatgattaa
     1861 tatcaccaga aagtttatag cgcgttgtcc ctaacgccgc agcaggggtt tgttttaata
     1921 attcaatttg cgcttcatct tcttgtcgta aatcaataag attgtcgttt aataaacctt
     1981 caataccgtg taaactacca taaattccat cgatatattc ggtttgccgt ttggcttctt
     2041 tgatgacacc atacattgag gtgttaatga ttgcggttgg tccgcctgat tggaaataca
     2101 ctaatgattt tttcatattt ttactccttt tctcacccga gattatacca tgagtgacta
     2161 ttaaagacta tcgatagcct gtaaagtatc aacaatttta ttaactctaa tcatcgcttc
     2221 ttctttcgta atcttcttac ttaagaattc atgaatgatt cttgcggttt cggcagcatt
     2281 aactttagtt aatccgcgtg ttgttaccgc cgcaaagcct aggcgaagac cagatgtcgt
     2341 atttggtctt tcatcatcct taggtaacat atttttattc gttgtaattt taattgtttc
     2401 aagtagtgct tcagcctctt ttccagtaat accatacgac ttttta
//
LOCUS       DAOSEX010000116         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_42471_length_2426_cov_2.668916, whole genome shotgun sequence.
ACCESSION   DAOSEX010000116 DAOSEX010000000
VERSION     DAOSEX010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42471_length_2426_cov_2.668916"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..385
                     /locus_tag="PK612_02775"
     CDS             125..385
                     /locus_tag="PK612_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95066.1"
                     /translation="MNINEEELNATFTASEYYEELNPTIKKRITDNFSFFVDGYLAVS
                     ILNSAINEIDSKLINTKELTKLLHKNELFKLEAENLVKYTKS"
     gene            719..1204
                     /locus_tag="PK612_02780"
     CDS             719..1204
                     /locus_tag="PK612_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95067.1"
                     /translation="MLIPFVVSAYLMQVFFLIGLFAGESFNWANYAGLVFTLLTLVFG
                     VIATVKSVTGDTKDTRKETMVFKLLLIPYFVINFIIGFMAVVGALVNFMVLPIIVAGV
                     ILMLVFNYFMVVVTSASNIRYLIKNLVVKKDPLTLLHIAFHFIFVTDVISSVILALKK
                     D"
     gene            1314..>2426
                     /locus_tag="PK612_02785"
     CDS             1314..>2426
                     /locus_tag="PK612_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95068.1"
                     /translation="MEQNFRARREKIFGRFKKEQVLRWMMSFTVTTSVAVTAVVVAST
                     AEPEVSFTSVEVLGNTVYYETLVNDPDLTLESESLVLDVKSGLETFSFPLSLGITTGN
                     FNIRYFNMEYELAIKGSQGYGKKTFATYNIISVSEPAARIMATHYTEERQAFTFTFDI
                     FIFNPDLELLDITLFPSFAFSEPGEEGVVTEIIELPRINLEEGTNVCTLESIPTHASF
                     VSLKIAGTKNDEEVILLDESIPLPSTFSSYIHAVGFSATSLSLVAEVFAPLLFEGTAT
                     LNLYEEETLIAAREFNVPYVDAEGEYIPPIFDFENLSVNTMYTAQIEIPYMNHIRKQN
                     EVNKSELQNVYTAPYYSYNAEHILYENEMVYITLDVV"
BASE COUNT          802 a          351 c          422 g          851 t
ORIGIN      
        1 gtgagcgatt aaagataatg agtcagctgt atacgctcct agttatatta aaagattctt
       61 taatgtcccg actaactata atgaagataa gtttagtatt tattacccta aattcactaa
      121 ttctttgaat attaatgaag aagaacttaa cgctacattt acagcttcag agtattatga
      181 agaactaaat ccaacaataa agaaaagaat aactgataac tttagtttct ttgtggatgg
      241 ctatctagca gtatctatct taaatagtgc aattaacgaa atagactcta aacttataaa
      301 tacaaaagaa ttgacgaaat tattacataa aaatgaatta tttaaactag aagcagaaaa
      361 tcttgttaaa tatactaaat cttaagagat tttgcccaaa tttataaata caatagaatt
      421 ttagagttta tgacaaaaaa aatatcattt tattttgaca taaaaccaaa aagcaaagta
      481 gtccgcaaaa aagtcaaatc tgtccaccaa aaatgaagaa atctgtccat taaatgttcc
      541 aaaagtctcc gtttggtgtt aaagaatgag aaaatatcat tcgtgtatat tggttaatat
      601 gatgattata tgacgtattc tgtttctttt agttaatgat tcaagtaaat gtataaatta
      661 tggcataata tggaagatag aaaacgttat atcaacacgt tttctggagg taagtaatat
      721 gttaattcct tttgttgtta gtgcttactt aatgcaagtg ttctttctaa ttggtttatt
      781 tgcgggtgaa agctttaatt gggcaaatta cgctggttta gtgtttactt tattaacttt
      841 agtgtttggt gttatagcca ccgttaagag cgtaacaggg gatacgaaag atacaagaaa
      901 agaaacaatg gtgtttaagt tactacttat cccttatttt gtgattaatt ttattattgg
      961 ttttatggct gtagttggtg cgttagttaa ttttatggtc ttacctatta ttgttgcagg
     1021 tgttatcttg atgcttgtct ttaattattt tatggtagtc gttactagcg ctagtaatat
     1081 tcgttattta attaaaaact tagtagtaaa aaaagatcct ctaactttat tacatattgc
     1141 ttttcacttt atttttgtca cagatgttat tagtagtgtt atcttagcgc ttaaaaaaga
     1201 ttaaatgctt acttaattca aaaatatatt tttatttaaa tatttgtcct ttaaagcatt
     1261 aatatttgct tgtaatttta tttattccag tagtaaaatg tttttaagca tatatggaac
     1321 aaaatttccg cgcccgtcgc gaaaagattt ttggacgatt taaaaaagaa caagttcttc
     1381 gctggatgat gtcttttaca gtgacgacta gtgtcgcggt tactgcggta gtcgttgcca
     1441 gtaccgctga accagaagta tcttttacta gtgttgaagt gcttggtaat acggtatatt
     1501 acgaaacact cgttaatgat cccgatttga cacttgaaag tgagtcactt gtacttgatg
     1561 taaagtctgg gttagaaact ttctcgttcc cgctttcttt aggaataaca acgggcaact
     1621 ttaatatccg ttattttaat atggaatatg aactagccat taaaggttca caaggctacg
     1681 gtaaaaagac ttttgcaact tataacatta ttagtgttag tgaaccggca gcgcgcatca
     1741 tggccactca ttacactgaa gagcgtcaag cctttacttt tacctttgat atatttattt
     1801 ttaatcctga tcttgaatta ttggatataa cgctattccc gtcttttgct tttagtgaac
     1861 caggtgaaga aggagtagtg acagagatta ttgaattacc acggattaat ttagaagaag
     1921 gcacgaatgt ttgcacactt gaatcaatac cgacgcacgc tagttttgtt agtcttaaaa
     1981 tagcgggcac taaaaatgac gaagaagtta ttttattaga tgaaagtata cctttacctt
     2041 caacattttc ttcttatatt cacgcggtgg ggtttagtgc gacttcactt tcactcgtcg
     2101 ctgaagtttt tgcgccacta ctttttgaag gaacagcaac actaaatctt tatgaagaag
     2161 aaacacttat tgcagcacgc gagtttaatg tgccttatgt tgatgctgaa ggagaatata
     2221 ttcccccaat ctttgatttt gaaaatttaa gcgtcaatac aatgtataca gcgcagattg
     2281 aaattccgta tatgaaccat attaggaaac aaaatgaagt aaataagagt gaattacaga
     2341 atgtgtatac agcgccgtat tactcttata acgcagagca tattttgtac gaaaatgaaa
     2401 tggtatatat cacacttgat gttgtt
//
LOCUS       DAOSEX010000117         2396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_43286_length_2396_cov_4.205041, whole genome shotgun sequence.
ACCESSION   DAOSEX010000117 DAOSEX010000000
VERSION     DAOSEX010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43286_length_2396_cov_4.205041"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK612_02790"
     CDS             complement(<1..218)
                     /locus_tag="PK612_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95069.1"
                     /translation="MSTKAGVLHLEKGPMQTKKTIAMPIWLKALIALLPALFFLLVFT
                     IFPIFNTAFIAFVKDFYFQSGTGSFALA"
     gene            complement(892..1671)
                     /locus_tag="PK612_02795"
                     /pseudo
     CDS             complement(892..1671)
                     /locus_tag="PK612_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012358655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1765..>2396)
                     /locus_tag="PK612_02800"
     CDS             complement(1765..>2396)
                     /locus_tag="PK612_02800"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOH95070.1"
                     /translation="GREDSLFTFDAINSEAPWEVPFKEGKQEAVKQFYTDEIEKAHQH
                     LDYHEVVVPLIIKAERLIFYAMAGSYLTSALILIVIIPNFRKDKVTFGKVITGTIVLN
                     RLGYKMSVLQANMRNFAIFIFSFVFFFVPFHLISFAMTLFSKKQLSMYDHLVATLVAD
                     KKATLVFKDVNEETKYRKELAQRLIAVDKRKAESREERLRDKEKLNKID"
BASE COUNT          693 a          498 c          430 g          775 t
ORIGIN      
        1 cttgcaagtg caaaactacc ggtcccgctt tggaaataaa agtctttaac aaaagcaata
       61 aatgctgtat taaaaatcgg gaaaatggta aagacaagca agaagaaaag ggcaggtaat
      121 aaagcaatta atgcttttaa ccaaattggc attgcaatcg tctttttcgt ttgcattggc
      181 cctttttcaa gatgcagtac tcctgcttta gtgctcatag cctacctcct ttcataaata
      241 agaatctttc atcgcgtttt cctataaaat ccgtgaacca tcaatatcaa agagatagaa
      301 gcgttttgga tataccttaa ttactttgtc tttgacttta tgacgttctt cagcagggat
      361 aataacgcga atccggtgtt tttgtcccac aacattagcg acgatcgtaa tatcgcggcc
      421 aatatgttca atataaagta cttcaatatc aattgctgga ttatcatttt tgtcaagggt
      481 gaatgcttca ggacgcacac caatcttcac atcacgattt gcaccagctt caacgatgag
      541 tttaccaacc tcttcttcaa aatcagcgta agcataacga tagccgccac cgacgatagc
      601 ttcataaatg ctttcaaggt taacttcacc ttcaataata cgcggaacaa atttttgttt
      661 tttctttgga tttaatttgg caaagaaatc actaactttt tctttaaatg ctttcttttc
      721 tggttcttct ttaacttctg gctctgcttc aacataatca acgcctaagc tagcgaagta
      781 atcagttttc gttccgttat aaccaccttc aacagcgata tcataaattc tttcatcaaa
      841 gtaatcaaat ttaccttttg atttaagccc ttcatgataa acattgccgt gaagtaatac
      901 tttaccattc tttaattcac catcaaaaat attaatcgct ggtgagccta agaatttcgc
      961 aacaaataag ttgactggtt cttcatacac cgtttgcggt gcatcgattt gatggacaac
     1021 accgcgacgc atcacaacga taaagtcact aatactcatt gcttcttctt ggtcgtgagt
     1081 aacaaagatt gttgtaactt ttgtatcacg ttgaatccgc cggatttctt cacgggtttg
     1141 aagccgtaag cgtgcgtcta ggttggagag cggttcgtca agtaataaaa cttggggccg
     1201 cttcgagagc gcacgggcaa tcgctacccg ttgttgttga ccacccgaaa gttgttttgg
     1261 taggcggtcg agcaagttac caatgtcgac taagtttgcg acttcttgag caatttgctc
     1321 catttcggtt ttttctagtt tcggtctgac attctcgagt gggaatgtaa tgttttgccg
     1381 aactgtaaaa tgcggataga gcgcatagtt ttgaaacact aaaccgatcc ctctattttc
     1441 aggcggaacc tcagtgacat cacgatcacc gaaccaaatc ttaccagcgg tcggtttgtg
     1501 taatccagcg atcatgtaga gtgtcgttga ttttccacag cccgaaggtc ctaaaaggcc
     1561 gacaagcttt ccatcaggaa tagtaaaatt catctgatca acagctttga ctgtcggagc
     1621 gcctttgact ccagggaagt gtttcgacag attatctaat ctaacttcca tatttcctcc
     1681 tgcagattgt ctgctatgtt tattttataa tagcacaata tcaaggtttt tggctattgt
     1741 tttattatcg gcattatgta gtttttaatc aattttatta agcttctctt tatcgcgtaa
     1801 tctttcttca cgactttcgg ctttgcgttt atcaacggca attaggcgtt gtgcgagctc
     1861 tttacgatac tttgtttctt catttacatc tttaaagaca agcgttgctt ttttatccgc
     1921 cacgagtgtt gccacaaggt gatcatacat actaagttgt tttttagaaa agagtgtcat
     1981 cgcaaaggaa atgagatgaa agggtacaaa gaaaaagaca aaactaaaga taaatatcgc
     2041 gaaattgcgc atatttgctt gcaaaacgct cattttatat cctaaacgat ttaagactat
     2101 ggtccccgta attaccttac caaatgttac tttatctttc ctaaagtttg gaataatgac
     2161 aataagaatt aaagcacttg ttaagtaact tcccgccatt gcataaaaaa taagccgttc
     2221 agctttaata atgagcggaa ctactacttc atgataatca agatgttgat gcgctttttc
     2281 tatttcatca gtgtaaaatt gcttcaccgc ttcttgcttc ccttctttaa aaggaacttc
     2341 ccacggcgct tcactattaa tagcgtcaaa agtaaataaa gagtcttcgc gacctt
//
LOCUS       DAOSEX010000118         2393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_43391_length_2393_cov_3.694183, whole genome shotgun sequence.
ACCESSION   DAOSEX010000118 DAOSEX010000000
VERSION     DAOSEX010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43391_length_2393_cov_3.694183"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PK612_02805"
     CDS             complement(<1..204)
                     /locus_tag="PK612_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the exonucleic cleavage of mRNA yielding
                     nucleioside 5'-phosphates; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH95071.1"
                     /translation="MKIKDLNDGIRVIENYLVTNVTKGVSNNGQMYLTVSLQDNTGQI
                     EARVWDANENDVEVFKQGNFVRVV"
     gene            complement(194..964)
                     /locus_tag="PK612_02810"
     CDS             complement(194..964)
                     /locus_tag="PK612_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOH95072.1"
                     /translation="MVKIINITLKKEQYNITFDNGEELLVSAETIAEFFLYNGKELSL
                     KDLAKIKEYEAQTKHFSYTSNLLARRPYTSHEIREKLRKRRVNEEVIDLIVDKLKRLN
                     LINDLRYALDKIQYLINVKKCSTRAIEQDLLRRGIHPYVIKDALAQSPRDERRELEIL
                     IPKLLKSYRNESLRRAYQKLKTKLYSLGFESDNIDEVLAKFNVEDYIDETENIKKALV
                     KLEKKAKGDTKVLYNKLVIAGYPASLIRKLLEDYSYEN"
     gene            complement(964..2133)
                     /locus_tag="PK612_02815"
     CDS             complement(964..2133)
                     /locus_tag="PK612_02815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH95073.1"
                     /translation="MRIAIFTDTFVPDTNGGAVSSYNLFKALNDRGHYCVVVTSNPFS
                     KEVTFIDNVLRIPGIEMKKLYGYNFAGPNRKAHKILKKLKIDIVHVQNEAGMGIFGKS
                     FARQQKIPYVYTYHTMYEDYTYYATKGRMETFAKAIIRRFSRWQAEIADEFIVPSDKT
                     KVSLREYGVQRYINVIPSGVDFSKFMYNPEDKPFLDEYKKEHGLDGTFNLVYVGRVAK
                     EKSIDVVIRGYALYVKKNLNHNTRLIIVGGGPAIPELEELAEKLQIAPFVHFVGKVLP
                     ADIGKYYHLGEVFVSASISETQGLTFMEAMAAERLLLCRFDEGLVGVIRDGESGFFFR
                     SEEDFGEMVKKIRSLSTKEIRNILDEANNIVDYYSNDRFVERCEHVYLRALRNSW"
     gene            complement(2133..>2393)
                     /locus_tag="PK612_02820"
     CDS             complement(2133..>2393)
                     /locus_tag="PK612_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95074.1"
                     /translation="WRVATFYVNLIVGGFVTAFYRSSMEEIVDETGHVKTFEEVKSYT
                     FAERRETSVTMYATSQLSIKEIQRRLSPKKKRRWRNHKEDQE"
BASE COUNT          729 a          499 c          350 g          815 t
ORIGIN      
        1 gacaacccgc acaaagttac cttgtttaaa tacttctaca tcattttcat ttgcgtccca
       61 tacccgcgct tcaatttggc ctgtattatc ttgcaaactg accgttagat acatttgacc
      121 gttatttgat acacctttag ttacattcgt cactaaataa ttttcaataa cacgaatgcc
      181 atcatttaaa tctttaattt tcataactat aatcctccaa caatttacga attaaggaag
      241 cgggataacc cgcgataact aatttattat acaacacttt agtgtcacct tttgccttct
      301 tttctagttt cactagtgct ttcttaatat tttctgtttc atcgatataa tcttctacat
      361 taaatttcgc taaaacttca tcaatattat cactttcaaa gccgagacta tataatttag
      421 ttttaagttt ttgataagca cggcgcagtg attcattacg atacgatttt agtaatttag
      481 gtataagtat ttcaagttca cgtcgctcgt cacgtggcga ttgcgcaaga gcatccttaa
      541 taacataggg gtgaatacca cgccttaata aatcttgctc aatagcacga gtagagcact
      601 ttttaacatt aattaagtac tgtattttat caagtgcata ccgtaagtca ttaattaaat
      661 taagacgctt aagtttatcg actattaaat caataacttc ctcatttaca cgccttttgc
      721 ggagtttttc acgtatttca tgacttgtgt aaggtcttct cgctaataag ttactagtgt
      781 aactaaaatg cttagtctgt gcttcatatt ctttaatttt cgctaagtct tttagtgata
      841 actctttgcc attataaaga aaaaactccg caattgtttc tgcacttact aatagttctt
      901 caccattatc aaaagtgata ttgtattgct ctttcttcaa tgtgatattt ataattttaa
      961 ccattaccaa ctattacgaa gggcccttaa atagacgtgc tcgcatcttt ctacgaaacg
     1021 gtcattactg taataatcaa ctatattgtt tgcttcatct aatatattgc gaatttcttt
     1081 tgtggataaa ctgcgaattt tcttcaccat ctcaccaaag tcttcttcac tacggaaaaa
     1141 gaaacctgat tcaccgtctc taatcacccc gacaagccct tcatcaaaac gacaaagtaa
     1201 aaggcgttct gcagccatcg cttccataaa ggttaagcct tgggtttcac taattgaggc
     1261 actaacaaac acttccccta aatgataata cttgccaata tccgcaggta agactttgcc
     1321 gacaaaatgg acaaacggag caatttgtaa tttttctgca agttcttcta attcaggaat
     1381 tgccggtcct ccgccaacga tgatgagtct agtattatga tttagattct ttttaacata
     1441 aagtgcataa cccctaatta cgacatcaat acttttttct tttgcaacac gtccaacata
     1501 cactaagtta aaagtgccgt ctaacccgtg ttccttttta tattcatcaa ggaaaggttt
     1561 atcttcagga ttatacataa attttgaaaa atcgacaccc gacggaataa catttatata
     1621 acgctgaacc ccatattcgc gaagtgacac ctttgtttta tcacttggta caataaattc
     1681 atcagcaatt tctgcttgcc atcgtgaaaa gcgacggata atagctttag caaatgtttc
     1741 catccgtcct tttgtcgcgt aataagtata atcttcatac attgtgtgat atgtatacac
     1801 ataaggaatc ttttgttgac gagcaaaact ttttccaaat atacccatcc ccgcttcatt
     1861 ttgaacatga acgatatcga ttttaagctt ttttaaaatc ttatgtgctt tacggtttgg
     1921 tcccgcaaag ttgtaaccat agagtttttt catttcaatt ccgggaatcc gcaaaacatt
     1981 atcgataaat gttacttctt tggaaaaagg attagaagta acaacaacac agtaatgacc
     2041 gcgatcgtta agtgctttaa aaagattata actagaaacg gcgcccccat tagtgtcagg
     2101 gacaaatgta tcagtaaata ttgcaattcg cattattctt gatcctcttt atgattgcgc
     2161 catcttcgtt ttttcttagg tgataaacga cgctgaattt ctttaattga taattgactt
     2221 gtggcataca tcgtcacact agtttcacga cgttcagcaa aggtgtaact cttaacttct
     2281 tcaaatgttt taacgtgacc cgtttcatca acaatttctt ccatactcga acgatagaag
     2341 gccgtcacaa atccgccaac gattaagtta acataaaatg ttgccacccg cca
//
LOCUS       DAOSEX010000119         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_43527_length_2388_cov_3.053150, whole genome shotgun sequence.
ACCESSION   DAOSEX010000119 DAOSEX010000000
VERSION     DAOSEX010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43527_length_2388_cov_3.053150"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PK612_02825"
     CDS             <1..575
                     /locus_tag="PK612_02825"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOH95075.1"
                     /translation="IKARKLKRENDDLCAIFVDYIGLITTTGHVESRQIELSEISAAL
                     KGLARELNITIVALSQLSRAVEATKDKMPQMSHLRESGSLEQDADLVFLMYREDYYVK
                     QGTIKEIDAVYQPYYQTLAQAKAEMKDPSKDLDVSPVDIILAKNRSGETGIATLLFFK
                     AISSFDNPGPDLTARFTEYRTKKGPSYKHE"
     gene            572..1330
                     /locus_tag="PK612_02830"
     CDS             572..1330
                     /locus_tag="PK612_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH95076.1"
                     /translation="MKYFVLASGSKGNATIIKGPETKILIDLGLSLKEYKERLNVFKL
                     QEERVFAVLFTHKHGDHITSQFNKVANGNVYAPANTLPFEAPFKLVVPYTPFTINEFK
                     VTPLPTSHDVSDSVGYIIETTEYKLVYMTDTGYISTRNLEMMKNADAYIIESNHSPSL
                     LLKTNRPFDLIQRILSDTGHLSNEACAHYLAELVGPRTKHIVLAHLSEEANTPEVALE
                     TLDKVFRKYGIDQSKLTIVTAKQHVPIMGELNEN"
     gene            1320..1796
                     /gene="rlmH"
                     /locus_tag="PK612_02835"
     CDS             1320..1796
                     /gene="rlmH"
                     /locus_tag="PK612_02835"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOH95077.1"
                     /translation="MKIDIIVVGKTKGALLITSDEYTKMISKYADISIIEIKEERLPL
                     RASEAQIENALNREAETILKRIGERDFVYALSPEGIEFDSIMFSEQLRNAFDIGQSKV
                     MFVIGSSHGLGNEVYKRANLKLSFSKMTFPHQLFRVMLLEQIFRAFKIINNETYHK"
     gene            1811..2188
                     /locus_tag="PK612_02840"
     CDS             1811..2188
                     /locus_tag="PK612_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95078.1"
                     /translation="MANNTYNYDNIVPEWNYSEFKHLKRVSNPRTAFARGYLFEEGEF
                     YIEPWFYTQLTRILERFRNEHDEIMDVFFNIARKGKYVLFTRDINEPIFDDENYLLVE
                     IEDIIEGAKLIIDDNSRGSDYGD"
     gene            2178..>2388
                     /locus_tag="PK612_02845"
     CDS             2178..>2388
                     /locus_tag="PK612_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95079.1"
                     /translation="MAIKGIFVTNISHNSPKFRVQVDEFLNKAKLIPNLELIEVKSQH
                     VLSYLNNNTPDFCLFWDKDIYLAKRI"
BASE COUNT          845 a          394 c          446 g          703 t
ORIGIN      
        1 ttatcaaagc acggaaatta aaacgcgaaa atgatgattt atgcgccatt tttgttgact
       61 atattggttt aattacgaca acgggacatg ttgaaagtag gcaaattgaa cttagcgaaa
      121 taagtgcggc cctgaaaggc ttagcgcgcg aattaaatat tacaatcgtt gccttatcac
      181 aactttcacg ggcagttgaa gccacaaaag ataaaatgcc acaaatgagt caccttcgtg
      241 aatcaggtag tctagaacaa gacgcagatt tagtgttttt aatgtatcgc gaagattatt
      301 atgtcaaaca aggaacgatt aaagaaatcg atgctgttta tcaaccctat taccaaacac
      361 tcgcacaagc taaagcggaa atgaaagacc cgagcaaaga tcttgatgtt tcaccagttg
      421 acattatctt agcgaaaaat agaagtggtg aaacgggaat tgcgaccctg ctatttttca
      481 aggctatttc aagttttgat aatccaggac cagacttaac agcgcggttt actgaatatc
      541 gcactaaaaa aggaccaagt tataaacacg aatgaagtac ttcgtcctcg ctagcgggtc
      601 caaaggtaat gccacaatta tcaaaggacc tgaaacaaaa atattaattg atttagggct
      661 cagccttaaa gaatataaag aacgactaaa tgtctttaaa ttacaagaag agcgtgtttt
      721 tgccgtgctt tttactcata aacacggtga tcacattact tcacaattta ataaagtcgc
      781 taacgggaat gtgtatgctc cggcgaacac tttgccgttt gaagcaccat ttaaactagt
      841 tgtgccctac acccctttta cgattaatga atttaaagta acgcccctac caacaagtca
      901 tgatgtaagt gattcagttg gttatattat tgaaacaaca gagtacaaac ttgtgtatat
      961 gactgacact ggttatataa gcactcgtaa cttggaaatg atgaaaaacg ctgacgccta
     1021 cattattgaa tcaaatcata gtccgagttt attacttaaa acaaatagac cctttgattt
     1081 aattcagcga atactaagcg atactggtca cttatcaaac gaagcttgtg ctcattattt
     1141 agcagaactt gttggcccgc gcactaaaca catcgtttta gcgcacctta gtgaagaagc
     1201 taatactccc gaagttgcgc ttgaaacact tgataaggta tttcggaagt acgggattga
     1261 ccaaagtaaa ctgacaattg ttaccgcaaa acaacatgtg ccaatcatgg gagagttaaa
     1321 tgaaaattga tattattgtt gtcgggaaaa caaaaggtgc acttttaatt actagtgacg
     1381 aatacactaa aatgattagt aaatatgcgg atatttcaat tattgaaatt aaagaagaac
     1441 gacttccgct tcgcgcaagt gaagcacaga ttgaaaatgc attaaataga gaagccgaaa
     1501 ccattttaaa aagaattggt gaacgcgact ttgtttatgc cttgtcacct gaaggaatag
     1561 agtttgattc aattatgttc agcgaacaat tacgtaacgc ctttgatatt ggtcaaagta
     1621 aagtaatgtt cgttatcggt agttcccatg gtttaggaaa tgaagtatat aagcgtgcga
     1681 acttaaaatt aagttttagc aaaatgacgt ttccccatca actttttaga gtaatgctcc
     1741 ttgaacaaat atttagagca ttcaaaatta taaataacga aacatatcat aaataaggag
     1801 aaatattaat atggcaaata atacttacaa ttatgacaat attgttccag agtggaacta
     1861 tagcgaattt aaacatttaa agcgtgttag taacccgcgg actgcttttg cgagaggata
     1921 tttatttgaa gagggtgaat tttatattga accgtggttt tatacacaat taacgcgaat
     1981 cttagaacgt tttagaaatg aacacgatga aataatggat gtattcttta atatcgctcg
     2041 taaaggtaaa tatgtattat ttactcgtga tattaatgaa ccaattttcg atgacgaaaa
     2101 ctatctttta gtggaaattg aagatataat tgaaggtgca aaattaatta ttgatgataa
     2161 ttcacgcggg agcgattatg gcgattaagg gcatttttgt cacgaatatc agtcataata
     2221 gcccaaaatt tcgcgttcaa gttgatgaat ttttaaacaa agctaaactt ataccaaatc
     2281 ttgaattaat tgaagttaaa agtcaacatg tcttaagtta tttaaataat aacacacctg
     2341 acttttgtct tttttgggac aaggatatat atctagcaaa acgtattg
//
LOCUS       DAOSEX010000120         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_43595_length_2385_cov_2.893133, whole genome shotgun sequence.
ACCESSION   DAOSEX010000120 DAOSEX010000000
VERSION     DAOSEX010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43595_length_2385_cov_2.893133"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /gene="smpB"
                     /locus_tag="PK612_02850"
     CDS             complement(<1..378)
                     /gene="smpB"
                     /locus_tag="PK612_02850"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOH95080.1"
                     /translation="MAKQKKTNNVITENRRASHDYFLSDFLEVGIVLKGTEIKSLRHS
                     GASLKDTYIVFRNGEAFIEGLYIAPYAFGNIFNHEPRRTRKLLMHKREIARYSANVQI
                     ASMTCVATKMYFVRGRVKLTIALG"
     gene            complement(396..1301)
                     /locus_tag="PK612_02855"
     CDS             complement(396..1301)
                     /locus_tag="PK612_02855"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOH95081.1"
                     /translation="MENYSHKYKISPELTLYGAYWGVKKPRANVLVITGMQEHAMRYT
                     EFAKFLNEAEFSVYSIDYFGQGENVTQGGHTLGVLPNHGFEKFVTALGTFVENLAKDK
                     LPLYVLGHSMGSFLAQRFVQRYPLLSKKVVIVGSNGPSALFGMGKAIAALTVNNRNRD
                     KESKLLASMAIGAYSKAVKNARTPADWLSYNEDNVNTYLAHPLDGKPSSKGFYLELLR
                     GTSNLYKKKHYVDVPRDLPIFIIAGAEDPVGANGKGIIKLGKFYAKLGFTNLEVKIYE
                     GMRHEILNETDRRDVYADVLSFLMR"
     gene            complement(1419..>2385)
                     /gene="galE"
                     /locus_tag="PK612_02860"
     CDS             complement(1419..>2385)
                     /gene="galE"
                     /locus_tag="PK612_02860"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOH95082.1"
                     /translation="GSHTVVALLEEGHEVVIADNLVNSQRNVVTKIKRITGVKPFFYK
                     VDVADKKSMRKVFSEHKFDAVIHFAGLKAVGESVQKPLLYYRNNLDSTLTLLELMKEF
                     DVKNILFSSSATVYGTPENVPLKEEDPIGGTTNPYGTTKLIIEQILMDFVHANEGFKA
                     VSLRYFNPIGAHPSKLLGESPNDIPNNLMPYISRVANGKLSHLNVFGDDYETPDGTGI
                     RDYVHVLDLANGHVLALKAFSFNHAYNVYNLGTGKGTSVLELVHLYEKANKLKIPYVI
                     GPRREGDIAASYADITKAKNELGFVPHYSVKDACRHAYLFEKKQK"
BASE COUNT          724 a          483 c          405 g          773 t
ORIGIN      
        1 accaagcgca attgtaagct tgacacgacc acgaacaaaa tacatttttg tggcgacgca
       61 tgtcattgac gctatttgca catttgcgct atagcgtgct atttcgcgtt tatgcattaa
      121 aagtttacgt gtcctgcgtg gttcgtgatt aaaaatatta ccaaaagcat atggagcaat
      181 atatagacct tcaatgaaag cttctccgtt acggaaaaca atataagtat ctttaagcga
      241 ggcaccactg tggcgaagcg acttaatttc tgtgcctttt aaaacaattc ccacctctaa
      301 aaagtcacta agaaaataat cgtgacttgc acggcggttt tctgttataa cattattcgt
      361 ctttttttgc ttcgccacta tagtgtatct ttcttttatc tcattaagaa acttaagaca
      421 tcagcgtaaa catcacgccg atctgtttca tttaatattt cgtggcgcat tccttcgtaa
      481 atcttcactt ctaaattagt aaaaccaagt tttgcataaa atttacctag tttaataata
      541 cctttcccgt tagcgcctac tgggtcttca gcaccagcaa taataaagat tggtaagtca
      601 cgcgggacat caacataatg ctttttctta tataaattac tcgtgccgcg taataattct
      661 aaataaaaac cttttgatga cggtttccca tcaagcggat gcgcgagata agtattgaca
      721 ttgtcttcgt tataactaag ccaatcagcg ggagtacgcg catttttaac agcttttgag
      781 taagcaccaa ttgccatact agcaagtaac ttactttctt tatcacggtt acgattatta
      841 acggtgagag cggcaatagc tttccccatt ccaaaaagtg cagaaggacc gtttgatcca
      901 acaattacca ctttcttaga aagaagcgga taacgttgca cgaagcgttg cgctaagaaa
      961 ctacccattg aatgccctaa aacataaagt ggtaatttat cttttgctag gttttctaca
     1021 aaagtaccaa gcgccgtcac aaatttttca aagccgtggt taggtaaaac acctaaagta
     1081 tgaccacctt gcgtgacatt ttctccttgt ccaaaatagt cgatactata tacactaaat
     1141 tccgcttcat ttaagaattt tgcaaattca gtgtagcgca ttgcatgttc ttgcatccct
     1201 gtgatgacta agacattcgc ccgcggtttt ttaacacccc aataagcacc ataaagtgta
     1261 agttcggggg agattttata tttgtggctg taattttcca tattacgtcc tccttttttt
     1321 aattttacca tttatttaca tattgcttac ataagtaaaa tgaaaatatt tatggttaac
     1381 cattactcct taattttttg atataaatat aaaaattatt acttttgttt tttctcaaat
     1441 agataagcgt ggcgacaagc atcttttact gaataatgcg gaacaaagcc taattcattt
     1501 ttagcttttg taatatcagc gtagcttgcg gcaatatcac cctcacgacg tggaccaatg
     1561 acatacggaa ttttaagttt atttgctttt tcataaagat gcactaattc gaggacgcta
     1621 gtgcctttgc ctgtccctaa gttataaaca ttataagcat gattgaaact aaaggcttta
     1681 agcgctaaaa catgaccatt agctaaatca aggacatgga cataatctct aattcctgtc
     1741 ccatcaggcg tctcataatc atcaccaaac acatttaagt gacttaattt cccgttagcg
     1801 acacggctaa tgtaaggcat taagttattg ggaatatcat ttggtgattc accaagtaat
     1861 tttgaaggat gggcaccaat tggattaaaa tagcgtaaac taactgcttt aaaaccttca
     1921 ttagcgtgca caaaatccat taagatttgc tcaataatta actttgtagt gccataggga
     1981 ttggtagtac cgccgattgg gtcttcttct ttaagcggga cgttttcagg ggtgccatat
     2041 accgttgcac tactggaaaa aagaatgttt ttcacatcaa attctttcat cagttccagc
     2101 agtgttaatg ttgaatctaa gttgtttctg taataaagga gcggtttttg cacactttca
     2161 ccaaccgctt taagcccagc aaaatgaatc acagcgtcaa atttatgttc gctgaaaacc
     2221 tttcgcatgc tttttttatc agcaacatcg actttataaa aaaacggctt tactcctgta
     2281 atccgtttaa ttttagtgac gacatttctt tgtgagttca ctaaattatc agcaataacg
     2341 acttcatgac cttcttctag tagtgccaca acggtatgtg aaccg
//
LOCUS       DAOSEX010000121         2373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_43921_length_2373_cov_3.554357, whole genome shotgun sequence.
ACCESSION   DAOSEX010000121 DAOSEX010000000
VERSION     DAOSEX010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43921_length_2373_cov_3.554357"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1826
                     /locus_tag="PK612_02865"
     CDS             75..1826
                     /locus_tag="PK612_02865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH95083.1"
                     /translation="MRKPKRIIAGLALSALLLTGCSKDLVADKKGNIVTVTIDGNPVQ
                     INAGDMFEDYLKSSDGLKDYYDAVYEVVVRALFNKEEQTTERTEIYDIAALSVNDVKE
                     QAKNNAKGGGKYDDELKKILKTKNVETLKELEEKLAYDEMQVRLKKQFFDGGEGAWIT
                     NAMEELIVGERNQAGDLILDENNNPVYEGYLNNKLPYHVRHLLVKVGAANNDFVTGKI
                     SKDDANKLSSVIVALANHQDTFGLLANRYSEDEDSARQFGDLGIMNFTTNFVNEFKLG
                     VYAYDALYNADGVMTPEAKAAKLKIPGTAKTKLDALGLDEIPYGAALKLAAVREIEKS
                     DAGKEVNDGDAAFFPRNIIFNKYFNKHNIGVITPNEVNVFDEVGVEKAEFAALPGFKT
                     LPELGGKKVLTDEKGNVILVVRAGSAGSYEGIHFIVVERSALYDTVNGVTLKDYYTIE
                     TPGTAAFPKDDAGNDIDAFVNFLALDARGTKERIDKIRNEVKAFDPTINDRIFQKLVT
                     YLDVKFHDAELKAALDKYLANQYARSQFQLDQDLDKLWSEWIEYLDQQEVARTDRLVP
                     ETYIDDFKAGNDYNYGG"
     gene            1831..>2373
                     /locus_tag="PK612_02870"
     CDS             1831..>2373
                     /locus_tag="PK612_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95084.1"
                     /translation="MKRKYLLGLAVAALLLTGCDKITAEPVDLDKTVLVDELSEVVNN
                     TLENVYKKYHDSANFKQFVLDEVLLTIAEHEFGPYDKLAADDPFKLAVDTRAKHYFYD
                     ELLSGSYTYRSIFDEKKYVVERVYGTENSYLVDANGNEVAIGAIDTLPFYKEGLFLPI
                     VNKDNFDDPAFKLVHIDYYKH"
BASE COUNT          798 a          473 c          475 g          627 t
ORIGIN      
        1 cgcttatttt tgttgaaaag gttggaagtt aagtatagaa tataaaagca tattattagg
       61 aggtatagca ttatatgaga aaaccaaaac gcattattgc tggtttagcg ctatcggcac
      121 tacttcttac cggatgtagt aaagatttag tcgcagacaa aaaaggcaat attgtgacgg
      181 tgacaatcga tggtaaccca gtacaaatta atgcagggga tatgtttgaa gactatttaa
      241 aatcctctga tggactaaag gattattacg acgccgtcta tgaagttgtg gttcgcgctc
      301 tctttaataa agaagagcaa actacagagc gaaccgaaat ctatgatatt gctgccctta
      361 gcgttaacga tgtgaaagaa caagcaaaga ataacgccaa aggtggtggc aagtacgacg
      421 atgaacttaa gaaaattctt aagactaaaa acgtcgaaac attaaaagaa ttagaagaga
      481 aactcgcata cgatgaaatg caagtccgcc tcaaaaaaca attctttgat ggaggcgaag
      541 gcgcctggat cacaaacgca atggaagaat taatcgttgg tgaacgaaat caagcaggag
      601 atttaattct tgatgaaaac aacaatcctg tttacgaagg atacttaaac aacaaacttc
      661 catatcacgt tcgtcactta ctcgttaaag tcggtgccgc aaacaacgac tttgttaccg
      721 gtaagatttc caaagacgat gcgaacaaat taagttctgt tatcgtagcc ttagcaaatc
      781 atcaagacac cttcggactt ttagcaaacc gctatagtga agacgaagat agtgcccgtc
      841 aatttggtga cttaggcatt atgaacttca ccacaaactt tgtgaatgag ttcaaattag
      901 gagtttatgc ttatgacgct ctttacaacg ctgacggagt tatgactcca gaagcaaaag
      961 cggctaaact taaaattcct ggcaccgcca aaactaaatt agacgcactc ggacttgacg
     1021 aaattcctta cggcgctgcg cttaaacttg ccgctgttcg tgaaatcgaa aagagcgatg
     1081 ctggtaaaga agtaaatgac ggtgatgccg ccttcttccc acgtaacatt atctttaata
     1141 aatactttaa taaacataac attggagtca ttactccaaa tgaagttaat gtctttgacg
     1201 aagtcggtgt agaaaaagct gaatttgctg cccttcctgg atttaaaact cttccagaac
     1261 tcggtggtaa aaaagtttta accgacgaaa aaggtaatgt tatcttagtc gtgcgcgctg
     1321 gttcagccgg aagttatgaa ggaatccact tcatcgttgt cgaacgcagt gctctttatg
     1381 acaccgtaaa cggcgttact ttaaaagact actacacaat cgaaacccca ggaaccgctg
     1441 ctttcccaaa agacgatgct ggtaacgata ttgatgcctt tgtcaacttc ttagcacttg
     1501 acgcccgtgg cacaaaagaa agaatcgaca agattcgtaa tgaagttaaa gcatttgacc
     1561 caacaatcaa cgatcgcatt ttccaaaagt tagtgacata tcttgatgtg aagttccatg
     1621 atgctgaact caaagcagcg cttgataaat atctagcgaa ccaatacgct cgttcccaat
     1681 tccaactcga ccaagatctt gataaattat ggagcgaatg gattgaatat ttagatcaac
     1741 aggaagtcgc acgtaccgac cgccttgttc ctgaaacata tattgatgat ttcaaggctg
     1801 gtaatgacta caattacgga ggttaataga gtgaaacgta aatatttatt aggcttggcc
     1861 gtcgctgcac ttctcttaac tggttgtgac aaaattaccg ccgaaccagt tgatttagat
     1921 aaaactgtgc ttgtcgacga attaagcgaa gttgttaaca acacattaga aaatgtctat
     1981 aagaaatatc atgactccgc gaactttaaa caattcgtat tagacgaagt gttacttacg
     2041 attgccgaac atgaatttgg tccatacgac aaattagccg cagacgaccc attcaaactc
     2101 gcagtcgata cacgcgcgaa acattatttc tatgatgaac tattaagcgg ttcatacaca
     2161 tatcgctcaa tctttgacga aaagaaatac gtcgttgaaa gagtatacgg aacggaaaat
     2221 agctacctag tggacgctaa cggcaacgaa gtcgccattg gtgcgattga taccttacca
     2281 ttctacaaag aaggcttatt ccttccaatc gtcaacaaag ataactttga cgatccagcc
     2341 tttaaacttg tccatatcga ttactataag cat
//
LOCUS       DAOSEX010000122         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_44781_length_2343_cov_3.188811, whole genome shotgun sequence.
ACCESSION   DAOSEX010000122 DAOSEX010000000
VERSION     DAOSEX010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44781_length_2343_cov_3.188811"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PK612_02875"
     CDS             complement(<1..546)
                     /locus_tag="PK612_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95085.1"
                     /translation="MKIYELLQENRRGHLITPSREYLFFQDHEALLTQVPELIPFINS
                     KDDFDLICANIIQSALLNYEALTNYWNNEVKPDTELPVKALFTYNNVPIYCPLFSVSN
                     NILIGNNLGSKLNLTHDAIDLFEAYNFSLFESQLTSLVLVGEDIHTRAYYHYDFHAIY
                     IINDQGRLDLKICLFDKHIKRP"
     gene            complement(533..1258)
                     /locus_tag="PK612_02880"
     CDS             complement(533..1258)
                     /locus_tag="PK612_02880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOH95086.1"
                     /translation="MSYRFFGRVRNNEVVLSDDDIFHLTKVLRLKKDETFEVVAGQSL
                     YLCTYESVNPFILNVLEKRKLPLEPLPRLTLGYALPKGDKLELVIQKAVEIGVSHIIL
                     IDSERSIGKIPATRLESRLVRFNKIIKSAAMQANRNYLPTLTEPLAFKEVLDLEFDTK
                     LIAHETANLPLKAVLQQDLGDNLLILVGPEGGFSEAEVTLASTKGFNVITFGDNILRS
                     ETAAIYALSVLHHYQKGAHHENL"
     gene            complement(1286..2101)
                     /locus_tag="PK612_02885"
     CDS             complement(1286..2101)
                     /locus_tag="PK612_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOH95087.1"
                     /translation="MEILKYIILGIIQGVTEIFPISSSGHLVIFQELLKLEQPGLVFE
                     MFTNTASFLALFVLFAKDIWELIKGFFLYIFKKEAREEYKDTFFYVLKLLVAVIPLGV
                     VGLIFKDEMGMIKNLLTVGIALFITGALLLTIFFTRNLIEEHDDITWKDAIVVGFTQA
                     FAVFPGISRSGSTIIGGRASRVSLKSILRFSFLAYIIISVPTSVLGAVDLVHISEEIN
                     WTGYILAFIFTFGATFLTGLFVMKKLKVEHLIYFGIYCLVVGILAFSAFFIMR"
BASE COUNT          806 a          458 c          349 g          730 t
ORIGIN      
        1 agggcgctta atatgtttat caaataaaca aatcttcaaa tcaagtcgtc cttgatcatt
       61 aataatataa atcgcatgaa agtcataatg ataataagcg cgcgtatgaa tatcttcacc
      121 aactaacaca agactagtaa gttgcgattc aaataaacta aaattatacg cttcaaataa
      181 atctatcgcg tcatgggtta aatttagttt acttcctaaa ttattcccaa ttaaaatgtt
      241 attgctaaca ctaaaaagcg ggcaatatat gggtacatta ttataggtaa aaagtgcttt
      301 tactggtaat tctgtatcag gcttaacttc attattccag taattagtga gtgcttcata
      361 atttaagagt gcactttgaa taatattcgc acagattaaa tcaaagtcat ctttactatt
      421 aataaaagga ataagttcag gaacttgcgt taacagtgct tcatgatctt ggaaaaataa
      481 atattcacga ctcggtgtaa taaggtgccc tcggcgattt tcctgtaata actcataaat
      541 tttcatggtg cgctcctttt tgataatgat gtaacacact taaagcatat atcgctgcgg
      601 tttcacttct aagtatatta tcgccaaaag taatgacatt aaagccttta gtactcgcta
      661 aagtaacttc cgcttcacta aatccacctt ctggaccgac taaaatgagt aaattatcgc
      721 ctaaatcttg ttgcaatact gctttaagcg gtaaattagc ggtttcatga gcaataagct
      781 tagtgtcaaa ctctaaatct aaaacttctt taaatgctaa gggttcagta agcgttggca
      841 gatagttacg attagcttgc attgctgcac ttttaataat cttattaaag cgtactaagc
      901 gactttctaa gcgcgttgca ggtattttac cgatactacg ctcactatca attaaaatga
      961 tgtgactaac accaatttcc actgcttttt ggatcactaa ttcaagttta tcgccttttg
     1021 gtaaggcgta acctagtgtt aagcgcggta agggctctaa cggtaacttt cttttttcta
     1081 aaacattaag tataaacgga tttacacttt cataagtgca taagtaaaga ctttgtcctg
     1141 ccactacttc aaaagtttca tcctttttaa gccttaatac tttcgttaaa tgaaaaatat
     1201 catcatcact taagactact tcattatttc taactctgcc aaaaaacctg taactcataa
     1261 ctttaaatcc acatatattt tagcattaac gcataatgaa aaaagcactg aacgctaaaa
     1321 taccaacgac taaacaataa atcccaaaat aaattaagtg ttccaccttt aactttttca
     1381 tcacaaatag tcccgttaga aaagtcgcac caaaagtaaa gataaacgct aagatgtaac
     1441 ccgtccaatt aatttcttca ctaatatgta ctaagtcaac tgcgcctaac acacttgtcg
     1501 gtacactaat aataatataa gcaaggaaac taaatcttaa tatacttttt agtgacacac
     1561 gacttgcgcg accaccaatg atggtggagc cgctccgcga aataccaggg aaaacggcaa
     1621 acgcttgggt aaagcccacg actattgcgt ctttccacgt tatatcatca tgttcctcaa
     1681 ttaaattacg cgtaaagaaa atagtaagta aaagcgcacc agtaataaat aaagcaatac
     1741 caacagttaa tagattctta atcatcccca tctcatcttt aaaaataagt ccgacaaccc
     1801 caagcgggat cactgcaact aaaagtttta aaacatagaa aaaggtgtct ttatactctt
     1861 cacgcgcttc tttcttaaag atatacagaa aaaatccttt aattaattcc catatatctt
     1921 ttgcaaataa cacaaataag gctaaaaaac tcgcagtatt tgtaaacatt tcaaaaacta
     1981 accctggttg ttctagctta agtaattctt gaaagatgac tagatggcca ctagagctaa
     2041 ttgggaagat ttccgtgacc ccttgaataa tcccaagaat aatatattta agaatttcca
     2101 taactttcac tcactttgct tattactttg taattatagc ctattcttca ttatttagat
     2161 gttaaaaaag gttaggacgt gcttatccta acctttggct tcaagtttat taactattag
     2221 gcatttatcc aaattgctgg aagaataccc attgctgcat cagtgacatt tatagcggca
     2281 agggcgccgt tataatttac ccgcgaagca cttgtctcac tagaatatgg ggaacgtgtc
     2341 cac
//
LOCUS       DAOSEX010000123         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_45858_length_2305_cov_3.633778, whole genome shotgun sequence.
ACCESSION   DAOSEX010000123 DAOSEX010000000
VERSION     DAOSEX010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45858_length_2305_cov_3.633778"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="PK612_02890"
     CDS             complement(<1..1575)
                     /locus_tag="PK612_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95088.1"
                     /translation="MKKRLLLLPLLAMLLGGCNGGSSATPSISTPESIPSSEEPSVES
                     SAESVSVSIDTTSYEIATTTNYSNRELDHSIGMTALDSLGTQKMLVVPVVFSDTAHRA
                     TEEVHTMINKAFFGASEETGWESVASYYHKTSFGKLTLTGEVLPYFYSQWSTHAFGAL
                     EVSPTQEYGEGYYWDQTHHLIEEIYNTLDAELFLEYDQDGDGHVDAFWMVYIAEIKAL
                     GGEDDPFWAYKFYWNRYANTEKPTPNTYAWASYKFADEGVGYNFNIPDAHTYIHETGH
                     MFGLPDYYDYDSKTAPAGALDMMDHNVADHNMYSKYRLNWSKPFVVTGNADIHLRPAE
                     STGDFILIKDGWNGHVYDEYILIEYYTPTGLNEKDSQGRGYETKSSNVSTKSFDINGV
                     RMWHVDSRLMTYVFDPESLELTNAFWTDEIVVGDPFSYTIIGPSNTTSRSSTPEGDSK
                     VNKLLHLMDATGTTSRVGNWLRTPTTAGSTALFQTGDVVAADGWKKYIQTSATFNDGT
                     AVGYSVEIGEMDDNGVQ"
     gene            complement(1716..>2305)
                     /locus_tag="PK612_02895"
     CDS             complement(1716..>2305)
                     /locus_tag="PK612_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOH95089.1"
                     /translation="DTLKQGDRITEGAVHPRELLAVSTITAVQNYLLNEVQKVYRISA
                     SVRISDKHLEIIINQMLNRVYIIDGGDTSLLPGQRITTHQFTAANKEALINGLHPAVA
                     TPLILGIKAAALASGSFLSAASFQETTRILTDATVKGKLDPLHGLKENVITGRLIPAG
                     RGILTKEEQEALLEDFSVADTLDKIKASYDNSSNS"
BASE COUNT          622 a          484 c          462 g          737 t
ORIGIN      
        1 ttggacaccg ttatcatcca tttcaccaat ctcgacactg taacccactg ctgtgccgtc
       61 gttaaatgtt gcgcttgttt ggatatattt cttccaccca tcagcagcga cgacatcacc
      121 agtttggaat aacgctgttg agcccgcagt ggttggtgtc cgtaaccagt taccaacacg
      181 tgaagttgta ccggtagcgt ccattaagtg taataactta ttaaccttgc tatcgccttc
      241 tggtgtgctg ctacgtgaag ttgtattact tggaccaata atagtgtaac taaatggatc
      301 accaacaaca atttcatcag tccaaaatgc atttgttaat tctaatgatt caggatcgaa
      361 gacataagtc atgagccgtg aatcaacgtg ccacattctc accccattaa tatcaaagct
      421 ttttgtcgat acgttacttg attttgtttc ataccccctc ccttgactat ctttttcatt
      481 aagtccggtt ggggtatagt attcaattaa aatatattca tcatacacat gtccgttcca
      541 tccgtcttta ataaggataa agtcaccggt tgattccgct ggacggagat gaatatccgc
      601 attaccagta acgacaaatg gttttgacca gttgaggcga tatttagaat acatattgtg
      661 atcggcaacg ttatgatcca tcatatcaag cgcgcccgct ggtgcggttt ttgaatcgta
      721 atcgtaatag tcaggtaagc caaacatatg tcctgtttcg tgaatataag tatgcgcatc
      781 aggaatattg aagttataac cgacaccttc gtcagcaaac ttataacttg cccacgcata
      841 agtatttggt gttggttttt cggtattagc gtaacggttc cagtaaaatt tataagccca
      901 gaacggatcg tcttccccgc ctaatgcttt aatctcggca atatacacca tccagaaagc
      961 atcaacgtgg ccgtcgccat cttggtcata ttctaagaat agttcggcat ctaatgtatt
     1021 gtaaatttct tcaattaagt ggtgcgtttg atcccagtaa taaccttcac cgtattcttg
     1081 cgtcggggaa acttctaatg cgccaaaagc atgggttgac cattgtgaat agaagtaagg
     1141 gagtacttca ccagttaaag ttaattttcc aaaactcgtt ttgtgataat aagaggcgac
     1201 tgattcccag cctgtttctt cacttgcacc aaagaaagct ttattaatca tcgtatggac
     1261 ttcttcagtt gcgcggtgcg cggtgtcact aaacacgaca ggaacgacta acatcttttg
     1321 tgtgccaagt gagtctaatg cagtcatacc aattgaatga tctaattcac ggtttgaata
     1381 atttgtcgtt gtcgctattt catagctcgt cgtatcaatt gaaacgctga cgctttctgc
     1441 acttgattca acacttggtt cttcactact aggaatgctt tctggggtgc tgatgcttgg
     1501 agtagcacta cttccgccgt tacacccacc tagaagcatt gctaatagtg gtaataatag
     1561 taatcttttt ttcataatca taccaccctt tctttttaat ggctttggca ttatatcatg
     1621 ctctaattac tagttaaatg ataaggttca tatatttata tgaaaccggt tactaaaaga
     1681 gagcaatatg tttttatatt gctcccttaa tttatttagc tgttactact attgtcgtaa
     1741 cttgctttaa ttttgtctaa agtgtcagcg acactaaagt cttcaagcaa ggcctcttgc
     1801 tcttctttcg ttaagatacc gcgaccagca ggaattaagc gtccagtaat gacgttttcc
     1861 tttaagccgt ggagtgggtc aagtttacct ttaacagtgg cgtcagttaa gatgcgtgtt
     1921 gtttcttgga aacttgcggc acttaagaag gagccacttg caagcgcagc agctttaata
     1981 ccaagaatta atggtgtcgc tacagctgga tggagtccgt taataagtgc ttctttattc
     2041 gccgcggtaa attgatgggt cgtaattctt tgtcctggta agagactcgt atcaccacca
     2101 tcaataatat aaacgcggtt gagcatttgg ttaataataa tttctaagtg cttatcacta
     2161 atgcggacag aagcactgat gcggtaaact ttttgtactt cgttaagtaa atagttttgc
     2221 accgcggtaa ttgtgctaac agcgagaagt tcacgcgggt ggacagcacc ttcagtaatg
     2281 cggtcccctt gtttaagtgt gtcgc
//
LOCUS       DAOSEX010000124         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_45923_length_2303_cov_3.450623, whole genome shotgun sequence.
ACCESSION   DAOSEX010000124 DAOSEX010000000
VERSION     DAOSEX010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45923_length_2303_cov_3.450623"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..751
                     /locus_tag="PK612_02900"
     CDS             104..751
                     /locus_tag="PK612_02900"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOH95090.1"
                     /translation="MVKATLETLYMSFVSLFISWMIAMPLGTLVSETRPGGLFPHKIA
                     NFVLNRLIDLGRSIPFILLVVFLFPFTRLILGTAIGTSAMIIPLTISAIPFEARLIEE
                     ILSEVPYATVEAAKIDGASNTKIIIHIKWASKVPYLVNSLSIALINIIGYSAMAGVVG
                     GGGLGNYAIVYGFQRFNWTIIAESMVIIIIIVSMIQIINNLLVRYLLWRCLCLKH"
     gene            736..1473
                     /locus_tag="PK612_02905"
     CDS             736..1473
                     /locus_tag="PK612_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOH95091.1"
                     /translation="MLKTLKFAVLVSLPVFVLTSCNKESKTIVVGASSTPHALILEEA
                     KDYVLSKGFKLDITIFNDYVLPNFALENGELDANYFQHKPYLEEFNSANGTHLISVKD
                     IHFEPMGIYAGTKSDISAFLKTDTIIVPSDKSNYDRAINLLKEHGMNEANIIEVEAQN
                     IPLMLSDCAFAVINGNYALSAGVVERCLITEDKDSDIAKRMANVIVVKKGNEKSAKTQ
                     ILIEALSQDNIETFIKNKFGDSVIYLG"
     gene            1490..2287
                     /locus_tag="PK612_02910"
     CDS             1490..2287
                     /locus_tag="PK612_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOH95092.1"
                     /translation="MAYEKTSPKHPDKMCDVIAGALVDMAYKKEENPRIAVEVLLGHG
                     HCHIINETSVKLDEKEVIKAVHRIVGRKVNVDYKEYPQDEHLSLNQKGKIRCGDNGIF
                     KGVPLTEEQKVLTKIAKEIYDIYPYDGKYILDNERLIICHSNVSTDELKKMFAYAEVN
                     PLGDWTGTEEVDAGVTNRKLGSDMADSVTGGGLHGKDLSKADVSVNIYAFLKAQETGE
                     VISLSCAIGDEYIDGKPYEEIVAIARRYIKSLGGFEEFAKWGLLRAE"
BASE COUNT          763 a          342 c          484 g          714 t
ORIGIN      
        1 tttagcacta aagtaagtga aacacagctt tataaaatgg ctggtaattc aattgtcgtt
       61 aactgtttaa cagcgatatt taaaaatctc tttggagcgt atcatggtta aagcgacact
      121 tgaaacttta tatatgtcct ttgtctcact atttatctcg tggatgatag caatgccact
      181 tgggacactc gtctcagaaa ctagaccagg cggactattc ccacataaaa ttgctaactt
      241 cgttttaaat cgacttattg atttagggcg tagtattccc tttatcttac tagttgtttt
      301 tctctttccg tttacaagat taatactagg cactgctatc ggaactagtg ccatgattat
      361 tcctttaacg attagtgcaa tcccttttga agcacggtta attgaagaaa tattgtcgga
      421 agtaccttat gcgacagttg aggccgctaa aatcgatgga gcatctaata cgaagattat
      481 tattcacatt aaatgggcga gtaaagttcc ctatttagtt aactcattaa gcattgcttt
      541 aattaacatc attggctact cagcaatggc aggagtagtt ggtggcggtg gattagggaa
      601 ttacgctatt gtttacggat ttcaacgctt taattggacg attattgctg aatctatggt
      661 gattatcatt atcatcgttt caatgattca aatcattaat aacttattag tgcgttattt
      721 attatggagg tgtttatgct taaaacatta aagtttgcgg tgcttgttag tttgccagtt
      781 tttgttttaa cgagctgtaa taaagaaagt aaaacgattg tagtaggtgc aagttccacg
      841 cctcatgccc ttattttaga agaagcaaaa gattacgttt tatcgaaagg atttaaactt
      901 gatatcacta tttttaatga ttatgtatta ccgaattttg ctttagaaaa tggtgagtta
      961 gacgctaatt actttcagca taagccgtat ttagaagaat ttaactctgc taacggtacg
     1021 catctaatct cagtaaaaga cattcacttt gaaccaatgg gtatttatgc aggtacgaag
     1081 agcgatatta gcgctttttt aaagactgat accattattg ttccaagcga taaatctaac
     1141 tatgatcgtg cgattaattt attaaaagaa cacggaatga acgaagcaaa tattattgaa
     1201 gtagaagcgc aaaacattcc gttaatgcta agtgattgtg cttttgctgt tataaacgga
     1261 aactatgcac tttcagctgg agtcgttgaa agatgtttaa ttactgaaga taaagattcc
     1321 gatatcgcta aaagaatggc taatgttatt gtggtgaaaa aagggaatga aaaaagcgct
     1381 aaaactcaga tactaattga agcactatct caagataata ttgagacttt tattaaaaat
     1441 aagtttggtg attcagttat ttacttggga taaggggagg aaatatttaa tggcttatga
     1501 aaaaacaagt cctaagcatc cagataaaat gtgtgatgtt attgctggtg cactagtaga
     1561 catggcttat aaaaaagaag aaaatccgcg aatcgcagtg gaggttttac ttggtcacgg
     1621 acactgtcac atcattaacg aaacgtcagt gaaattagat gaaaaagaag tgataaaagc
     1681 agtgcatcgt attgtggggc ggaaggtaaa tgttgattac aaagagtatc cacaagacga
     1741 gcatttaagc cttaatcaaa aaggaaaaat tagatgtggc gataacggta tatttaaagg
     1801 cgttccgctt acggaagagc aaaaggtact tacaaaaatt gcgaaagaaa tctacgacat
     1861 ttatccttat gacggcaaat atattcttga taacgaaaga cttatcattt gtcatagtaa
     1921 tgttagtact gatgaactta aaaaaatgtt cgcatatgct gaagtaaatc cactaggcga
     1981 ttggacggga acggaagaag ttgatgcagg ggtaacgaat agaaaactcg gtagcgatat
     2041 ggctgactcc gtaactggtg gagggttaca cggtaaggat ttatctaaag ctgacgtaag
     2101 cgttaatatc tatgcgttct tgaaggcgca agaaacgggt gaagttatca gccttagctg
     2161 cgctattggt gatgaatata ttgatggaaa accatatgaa gaaatcgtag cgattgcgag
     2221 aagatacata aaatctcttg gtggatttga agaatttgct aagtggggat tactccgtgc
     2281 tgagtaaatg taaagcaatt aat
//
LOCUS       DAOSEX010000125         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_45935_length_2303_cov_1.822509, whole genome shotgun sequence.
ACCESSION   DAOSEX010000125 DAOSEX010000000
VERSION     DAOSEX010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45935_length_2303_cov_1.822509"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PK612_02915"
     CDS             <1..471
                     /locus_tag="PK612_02915"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOH95093.1"
                     /translation="EFHPAQSALLKVQGKVVGVFGKLHPLAREKALLNNEAIIGELNL
                     SALLSLKTSLNKVNPPARFPFVRRDLALLVNEDLSVKELINVIRKAGGKLISDVSVFD
                     VYTKLKDEPGKKSVALTLTLQDANKTLVEDEIKEVMTKVIEELTKKLQVVVRGI"
     gene            468..974
                     /locus_tag="PK612_02920"
     CDS             468..974
                     /locus_tag="PK612_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95094.1"
                     /translation="MKIKIADGIKLHQTLTAEVDLSDVDLHANYSLKAIKPFTVTVDF
                     DTFTDVVDVHIRGKVTLILECAYTLEHFDYEVEMDDELTFNFVNPNIEAETDDCFYEK
                     GPEIFLDPYLYAIILSYIPLKVIKPGANLPKSGEGYEILSEEEFEAKHQTLDDEFGDL
                     FDKLDLDD"
     gene            1065..1541
                     /locus_tag="PK612_02925"
     CDS             1065..1541
                     /locus_tag="PK612_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOH95095.1"
                     /translation="MYNVVKESGRKWEMKFFGTYYHSLDAKGRLVVPARFRETLKDLK
                     TLYILQGFDGAISVYPEEAYHQELSFLEAQNFKNADARAYIRTIYASIEELEVDAAGR
                     ITLPVRIQKKYDIGQKVIVIGVGDHLEIWDEERYLSYERDACGNLEDLAAKLQEKA"
     gene            1541..>2303
                     /gene="rsmH"
                     /locus_tag="PK612_02930"
     CDS             1541..>2303
                     /gene="rsmH"
                     /locus_tag="PK612_02930"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOH95096.1"
                     /translation="MSTHYPVLLNEVISGVNVKDDSIIVDLTLGRAGHSSALLFKARN
                     GYLYGFDQDETAINESREILSETFQNFTLFHQNFKDFDIALEALGVTGADIILLDLGV
                     SSPQFDVSDRGFSYRYEGPLDMRMDRTNNTLTAAIIVNTYSEQKLKEIFYRYAEHRYS
                     ARIAREIVNARNEQEIRTTTELVEIIKRALPSKELRKKGHPAKTIFQALRIEVNNELG
                     VLEKVLERAPHFLNEGGRLAVISFHSLEDRLVKNVF"
BASE COUNT          780 a          399 c          472 g          652 t
ORIGIN      
        1 gagttccatc ctgcgcaaag cgcactctta aaagttcaag gtaaagtagt tggtgttttt
       61 ggtaaactcc acccccttgc cagagaaaaa gcattactta ataatgaagc aattattggt
      121 gaacttaatt taagtgcatt gcttagtctt aaaacaagct taaataaagt taacccaccg
      181 gcaaggttcc cgtttgtaag gcgtgactta gcgctccttg ttaatgaaga cttaagtgtt
      241 aaagaattaa ttaatgttat tcgtaaagct ggtggtaagt taataagtga cgtcagtgta
      301 tttgatgttt acacgaaact aaaagatgaa ccgggtaaaa aatcagtagc gctgacactt
      361 actttacaag acgctaataa gacattagtt gaagatgaga ttaaagaagt gatgactaaa
      421 gttattgaag aattaacgaa aaaattacaa gtagtggtac gcggaatatg aaaataaaaa
      481 tagctgatgg aattaaactc catcaaactc taacagcaga agttgattta agtgatgttg
      541 atttacacgc taattattcg cttaaagcga ttaaaccttt taccgtaact gttgactttg
      601 atacatttac cgatgttgtg gacgttcata ttagaggcaa agtcacactt attttagaat
      661 gtgcctatac tttggaacat tttgattatg aagtagaaat ggatgacgaa cttactttta
      721 actttgttaa tccgaatatt gaagcagaaa cggatgactg tttttatgaa aaaggaccgg
      781 aaatcttttt agatccatac ttatatgcaa ttatcttaag ttatatcccg cttaaagtta
      841 ttaagcctgg cgctaattta ccaaagagtg gcgaaggtta tgaaatctta agtgaagaag
      901 aatttgaagc taaacaccaa acgcttgatg atgaatttgg tgatctcttt gataagctag
      961 atttagacga ttaattatta ttctcataga atgagcataa aaggaggggc aactcttctt
     1021 ttttattttt tagtgttttt tttgtttaca acgaaaggtc atttatgtat aatgtagtca
     1081 aagaaagtgg gagaaagtgg gagatgaagt tttttgggac ttattatcac agtttagacg
     1141 ctaagggacg gcttgtcgta cctgctcgtt ttcgtgaaac acttaaagac ttaaaaacgc
     1201 tttacatctt acaaggcttt gatggggcaa taagtgtgta tcccgaagaa gcttaccacc
     1261 aagaactttc tttccttgaa gcacaaaact tcaaaaatgc tgacgctaga gcgtatattc
     1321 gtacaattta cgcaagtatt gaagagttag aagtagatgc agcaggccgt attaccttac
     1381 cagttcgtat tcaaaagaaa tatgacattg ggcaaaaagt aattgtaatt ggcgtcggtg
     1441 atcatcttga aatctgggat gaagaacgct acttaagtta cgaaagagat gcgtgtggta
     1501 acttagaaga cctagctgct aagttacagg aaaaagccta atgagtacgc attatccagt
     1561 attacttaat gaagtaataa gtggcgtaaa tgtcaaagat gatagtatca ttgttgactt
     1621 aaccttagga cgagcggggc atagcagtgc cttactcttc aaagcccgca acggctatct
     1681 ctacggcttc gaccaagatg aaactgcgat aaatgagtcg cgggaaatcc tcagtgaaac
     1741 attccaaaac ttcacgctct tccatcaaaa cttcaaagac ttcgatattg cgctagaagc
     1801 acttggagtc acaggcgctg acatcatctt acttgactta ggtgtcagta gtccgcaatt
     1861 cgatgtaagt gaccgcggct ttagttaccg ctatgaaggt ccacttgata tgcgaatgga
     1921 ccggacgaac aataccttaa cagcagcgat tattgtcaac acttattcag aacaaaaact
     1981 aaaagaaata ttctaccgct acgcggaaca tcgctacagt gcccgcattg ctcgcgaaat
     2041 cgtaaatgcg agaaacgagc aagaaataag aacaacaacc gagttagtcg aaattataaa
     2101 aagagcctta ccaagcaaag aattacgcaa gaaaggccat cccgctaaaa cgatcttcca
     2161 agcacttcgc attgaagtaa ataacgaatt aggagtactt gaaaaagtct tggaacgcgc
     2221 tccacacttc ttaaatgaag gggggagact cgcagtaata agcttccata gtcttgaaga
     2281 ccgcttagta aaaaatgtct tta
//
LOCUS       DAOSEX010000126         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_46322_length_2289_cov_3.662936, whole genome shotgun sequence.
ACCESSION   DAOSEX010000126 DAOSEX010000000
VERSION     DAOSEX010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46322_length_2289_cov_3.662936"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..294
                     /locus_tag="PK612_02935"
                     /pseudo
     CDS             1..294
                     /locus_tag="PK612_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016381313.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            1253..2284
                     /locus_tag="PK612_02940"
     CDS             1253..2284
                     /locus_tag="PK612_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOH95097.1"
                     /translation="MRKFDYSFLKDGSVPASLIGLISSIYSLRTLDETRKHKYASIYT
                     KLIKIAKVQSVKGSNAIEGIVTSDERIKAIVNQNSAPLNHDEEEIAGYRDALNIIHTT
                     YENLQFEEKTILDLHRLLLAVSSPENAGRYKTSDNVIIEIKPDGTRNVRFRPTPTSET
                     APAIEQLILAYTIAVAESGINKLLLIPCVILDFLCIHPFSDGNGRVSRLLTLLLLYKA
                     GFDAAKYISFEEQINKRKGFYYEALRKSSVGWHENNNNYFFFIENFLVTLFYCYQELD
                     KRFLTVNSEKVNKTTRKALYAALKKLMPDETFFIKKLEGQRFEILHKHQRVCVVKQIA
                     RDEIEVIYG"
BASE COUNT          861 a          329 c          415 g          684 t
ORIGIN      
        1 gcgcttggaa gagcgattgt taaagcttcc cctgtatttt taatggatga gccactatct
       61 aacttagacg ctaaattacg gattcaaatg cgcagtgaga ttgtttcgat tcataatcgg
      121 attggagcaa cgacagttta tgtaactcat gaccaaactg aagcaatgac aatggcaacg
      181 cgtattgtcg taatggataa agggcatgtt caacaaattg gcacaccaga agaagtttat
      241 tcacatccga acaacttatt tgtagcaaca tttattggtt cgcctgcgat gaatgctgtt
      301 aacgctattt atgataatgg ggttattaaa tttgaagatg gttacaaatt ctcattagat
      361 aaagataaaa ataatttagt catgtcattt atcaataaac aggtaaaaga atatgaagag
      421 ttgttgctag aaacatccaa aaaattagac aaacgaaaac tttatctgtc ttcaaaagcc
      481 gaacgctata aagttaactc cttgtttaaa cttgaaagaa agtatgcacg aaaagaaggt
      541 ttgcaccgca caaacatttc taacctagaa aaagctttag cggatggtga caccgatggt
      601 attcaagaag gaattgatta cttgaaaaga aaattagatg tgcttcttac ccgcaaagac
      661 actgaattaa aaacaataaa tagtaattat caaactttgc ttgcaaaaga agcaggaaca
      721 gaagataaac atgcaaggca aagaatagaa aaagacgatg aaattgaagt ttataatcaa
      781 gaaataactg attatatgaa aaatatcgat tattataagg aatgtaaaac gaaaaaaaga
      841 ctaccgataa aattcggtat ccgtccagaa gatattaaac caaagattgc tggtgaaacc
      901 ttcgaaaatg aagcaccaga aataaaagca acaatcgatt ttgttgaatt gttgggttat
      961 gaatattatt tatactttaa atttaatggt atgaaattta tttcaaaagc gaatgttggc
     1021 aagcataaat atgtaatggg agaagaacat acactaacat ttgatgaaaa taagatgagc
     1081 ttctttgatt ttaaatctgg tctttcatta tttaaaatta atacttaaat tatgtaaaag
     1141 aaaatttctg cataaaaatt tatcaaaatt atgcagacga ccacataaaa taattgcatc
     1201 tctgcataat aattagtaaa atatatgcag agaataaagg ttgttatgca gtatgagaaa
     1261 atttgattat tcgtttttaa aagacgggtc cgttccagcg agcttaatcg gattaatttc
     1321 gagtatttat tctttacgaa cacttgatga aacaagaaag cataaatatg catcaattta
     1381 tacaaagctt ataaaaattg ctaaagttca gtcggttaaa ggtagtaatg cgattgaagg
     1441 gattgttact tctgacgaaa gaattaaagc aattgttaat caaaatagcg cgcccttaaa
     1501 tcatgacgaa gaagaaattg ctggatatcg cgatgcatta aatattattc atactacata
     1561 tgaaaattta caatttgaag aaaagacaat attagattta catcgattgc ttttagcggt
     1621 ttcaagtcct gaaaatgcag gtcgttataa aactagtgat aatgtaataa tagaaattaa
     1681 acctgatgga acaagaaatg ttaggtttag accgactccg acaagtgaaa ccgcacccgc
     1741 aattgaacaa ctaatacttg cttacactat tgccgttgct gaaagtggga ttaataaatt
     1801 attgttgatc ccgtgcgtga tattagattt cttgtgtatt catccattta gtgatggtaa
     1861 tggacgggtt tctagactct taacattatt attgctttat aaagctggtt ttgacgctgc
     1921 caagtatatt tcttttgaag agcaaattaa taagagaaaa ggattttatt atgaagcact
     1981 taggaaatcc tcggttggct ggcatgaaaa taataataac tatttctttt ttattgagaa
     2041 ttttttagta acgctctttt attgttatca agaattagat aaaaggtttc taaccgtaaa
     2101 cagtgaaaaa gtcaataaga ccacgcggaa agccttatac gccgctctta agaaactcat
     2161 gcctgatgaa actttcttca tcaagaaact cgaaggtcaa cgcttcgaga tacttcataa
     2221 gcaccaacga gtgtgcgttg ttaaacaaat cgctcgcgat gaaatagaag taatttatgg
     2281 ataaggaga
//
LOCUS       DAOSEX010000127         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_46334_length_2289_cov_2.166070, whole genome shotgun sequence.
ACCESSION   DAOSEX010000127 DAOSEX010000000
VERSION     DAOSEX010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46334_length_2289_cov_2.166070"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /gene="deoC"
                     /locus_tag="PK612_02945"
     CDS             complement(<1..495)
                     /gene="deoC"
                     /locus_tag="PK612_02945"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOH95098.1"
                     /translation="MKKNKLIDHTLLKADASPAQIEKLCQEALEYDFMSVCVNPGYVP
                     LASMLLAESDVRVCTVVGFPLGATSTEAKVLEAQIAVEEGADEVDMVINVGKLKAGLD
                     LEVFTEIRAIREALPDWVILKVIIETCLLTDDEKVRACLLSRKAGADFVKTSTGFSTG
                     GATVA"
     gene            complement(715..918)
                     /gene="rpmF"
                     /locus_tag="PK612_02950"
     CDS             complement(715..918)
                     /gene="rpmF"
                     /locus_tag="PK612_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOH95099.1"
                     /translation="MAVQQRRGSKTRKRLRRTHFKLNVTGLTTCPNCGTLIRAHHVCP
                     ECHHYAGKDVITKEEPKKGKAKK"
     gene            complement(1057..>2289)
                     /gene="mtaB"
                     /locus_tag="PK612_02955"
     CDS             complement(1057..>2289)
                     /gene="mtaB"
                     /locus_tag="PK612_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOH95100.1"
                     /translation="FSIEETLKEQGYKLAHQGDKVDVAIINTCSVTSNADQKSRQHIR
                     KLAKNYPDAILVVMGCYAQLNKDLINTIPEIDILVGTSNRSKIVDYIEEFKEHGQKII
                     AVEEEPRTFNYEELGATSVTPQARAYLKIGDGCDAFCTYCIIPLARGKMRSRDVRDIV
                     KEAKDLVRRGYKEIVLTGIHTGAYGKDLGNIKFSNIVEMILEACPSLHKLRVSSIEAN
                     EIDDDFLRLMLKDKCIARHLHIPLQSGSDEVLRRMNRHYTTSEFIHKIENIREKVPNI
                     AITTDVIVGFPGESEKLFNETVAFIKKVNFTQLHVFPYSARTRTPAASFKEQVLGPVK
                     KERVRTLINLSEDLYRSYAQRFVGEKMQVLIETYDDKEEVYVGHTTNYLAVRVKSDSD
                     IKHEVITIIYDPNISKKI"
BASE COUNT          657 a          466 c          365 g          801 t
ORIGIN      
        1 ggctactgtg gctccgccgg tactaaaacc agtactagtt ttcacaaaat cagcaccagc
       61 tttacggctt aaaagacacg ctctaacttt ttcgtcatca gttaataaac atgtttctat
      121 aataacttta agaatgaccc aatcaggaag tgcttcacga atcgcccgga tttcagtaaa
      181 gacttctaaa tctaagccag ctttaagttt accgacatta attaccatat caacttcgtc
      241 agcaccttct tcgaccgcaa tttgcgcttc aagaactttc gcttcagtgc ttgttgcacc
      301 aagtgggaag ccaacaacag tacacacacg gacatcactt tccgctaaaa gcatactcgc
      361 aagtggaacg taccccggat ttacgcatac actcataaaa tcgtattcaa gcgcttcttg
      421 acaaagtttt tcgatttgtg cgggtgaagc atccgctttc agtaaagtat gatcaattag
      481 tttattcttt ttcatattac cctccaaatg atattttacc attttagcta atatttaatt
      541 acttttaata tataaaacac ctaaaacaac tatttttcta ctttataaga agttttgtaa
      601 aaggattttc aaaagtgcta aaagttcaag agttttgtaa atggattttc aaaactttgg
      661 attatttaaa ataaaaaagg aagtttcctt cctcatttat ttagcggtta atacctattt
      721 ctttgctttg cctttttttg gttcttcttt agtaatcacg tctttaccag catagtggtg
      781 acattctgga caaacatgat gcgcccgaat gagtgttcca cagtttgggc aagtggtaag
      841 tccagtcacg tttaacttaa agtgagtccg gcgtaagcgt ttgcgggttt tactaccacg
      901 tcgttgttga acagccataa acttatcctc cttcttgtga ctagggttat tatagcaaaa
      961 gaaaaagtag tgtcaagaaa tttagacatt ttatagtatt tattttatta tattgattat
     1021 atgctcataa tttagtgcat tatttttata cagtgtctag atttttttac taatattcgg
     1081 gtcataaata atagtaatta cttcatgctt aatatcgcta tcgcttttaa ctctaactgc
     1141 taaataatta gtggtatgcc caacatatac ttcttcttta tcgtcataag tttcaattaa
     1201 aacttgcatt ttttcgccga caaatctttg tgcataactt cgatataagt cttcacttaa
     1261 gtttattagt gtacgcaccc gttctttttt aactggacca agcacttgct ctttaaaact
     1321 agcggcaggc gttctcgtcc gcgcactata ggggaagaca tgtagctgcg tgaagtttac
     1381 ttttttaata aacgcgactg tttcattaaa taacttttca ctttctccag gaaagccaac
     1441 gataacatca gtggtaatag caatattagg tactttttct ctaatatttt cgattttatg
     1501 tatgaattca ctcgtggtat aatgccgatt catcctgcgg agcacttcat cacttccact
     1561 ttgcagcggg atatggagat ggcgtgctat acacttgtct tttagcatta aacgaaggaa
     1621 atcgtcatca atttcatttg cttcaatact tgatactcgt aatttatgta atgatggaca
     1681 agcttctaaa atcatctcca caatattact aaatttaata ttccctaagt ctttaccgta
     1741 ggctcctgta tggattcccg ttaaaacaat ttctttatac cctcttctta ctaagtcttt
     1801 agcctcctta acaatgtcac gcacatcgcg acttcgcatc ttccctcttg caagaggaat
     1861 aatacaatac gtacaaaaag cgtcgcaccc gtcccctatt tttaaatacg cgcgcgcctg
     1921 tggtgttaca ctcgtagcgc ctaactcttc atagttaaat gtgcgtggct cttcttcaac
     1981 cgcaattatt ttttgaccat gctctttaaa ttcttcaata taatcgacga ttttacttcg
     2041 gttactagtg ccgactaaaa tatcaatttc cggaatggta ttgattaaat ctttgttaag
     2101 ctgtgcgtag catcccatta caactaaaat agcatcagga taatttttcg cgagtttacg
     2161 gatatgttgt ctgctttttt gatcagcgtt acttgtgacg ctgcatgtat taatgatagc
     2221 aacatcaact ttgtcacctt gatgagcaag tttgtaacct tgttctttta aagtttcttc
     2281 aatactaaa
//
LOCUS       DAOSEX010000128         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_46344_length_2288_cov_8.840573, whole genome shotgun sequence.
ACCESSION   DAOSEX010000128 DAOSEX010000000
VERSION     DAOSEX010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46344_length_2288_cov_8.840573"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PK612_02960"
     CDS             complement(<1..761)
                     /locus_tag="PK612_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95101.1"
                     /translation="MSRKFKQKPKKVKAEKVKREPDMRKRAYLAMLFNNRAAFDGGRR
                     EPWWVAVLFFIASIVIALVPAMVQVGKTKGSDIFKGPLYHTDVAFTKFVETLEEKDAD
                     LTVVTENDENIFKASPEFVNLVATKTFTLTDGATNEVVPYYSFAQKRTVYTRDENNAV
                     VTNEVDFEYLRVYYTGDIQSSFLLEGKVYNGDAFLALKLLSLKEEDAVGNVTSHLIIG
                     RKALYTRLYNPTAINKPGNPALVFEGRTNSLPVGM"
     gene            1011..2186
                     /locus_tag="PK612_02965"
     CDS             1011..2186
                     /locus_tag="PK612_02965"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOH95102.1"
                     /translation="MYVIGIDVSKYKHDCIIMHEDTGEIVRDVFSFDNSRAGFVQFIS
                     VLKTLNPTLKKKIGFETTGHYQDNLKMFLADNGLDFMELNALLVHKFKSSLTLRGVKT
                     DKTDARLIANYLCKVEFKPYPKIVYNILSLRSLCKRHFTLIKDRTKYLISITNFLDRT
                     FPELKPFLNDNIKSKTAMFLLRKYGIPSKIANMNIQSYELLRKLSRGKFSVAKFYKLK
                     DLAKHTIGFTYPTDTFEIKSLLNVYDSINEEILKVETKISTLMSKINSKIPTIKGIGL
                     VSAATIIAEIEDINRFVSPDKLLAYAGLDPRIYESGTMSSKGKMNKKGSVNLRRVLMN
                     CAESVIMFNPAFYAYYRKKRDEGKAHRVAISHLARKLVRIIFKLETTQVDFDPSLVK"
BASE COUNT          699 a          452 c          399 g          738 t
ORIGIN      
        1 ttcataccaa ctggtaatga atttgtccgt ccttcaaaca ctaaagcagg gttaccaggt
       61 ttattaatgg ccgttggatt ataaagtctt gtgtataatg ccttccgccc aataattaaa
      121 tgtgaagtaa cattaccgac agcatcttct tcctttaatg ataaaagttt aagcgctaaa
      181 aatgcatcgc cattatagac tttaccttca agtaaaaacg aactttgaat atcaccagtg
      241 taataaacac gtaaatattc aaaatcaact tcattagtaa caacagcatt attttcgtca
      301 cgcgtataaa ctgtacgctt ttgcgcgaag gagtaataag gaacaacttc attcgtggcg
      361 ccatcggtca aagtaaaggt tttagtcgcc actaggttaa caaattcagg actagcctta
      421 aagatattct catcgttttc agtgacaaca gtaagatcag cgtctttttc ttcaagcgtt
      481 tccacgaact tcgtaaacgc tacatcagtg tgatacaaag gtcctttaaa gatatcgctt
      541 cctttagttt taccaacttg taccattgcc ggcactaaag caataacgat tgaagcaata
      601 aaaaagagca ctgcgaccca ccacggttcg cgtcttccgc cgtcaaaagc agcgcggtta
      661 ttaaaaagca tggctaagta agcacgttta cgcatatccg gttcgcgttt tactttttct
      721 gcttttactt ttttaggttt ttgtttaaat tttctactca tatgtatata cgtattattc
      781 agttaagcct agtttgatga ctgaataact tctctccttt ctaggctaca atgtaatagc
      841 tgtgcacctg tctcatttgt ttgccacttg atggcttgga ggtttttcag ggcacggctt
      901 tctatcacca tcgttaataa gttaagtaac gcttagacgt ttcatcattc gcgtttatac
      961 gaagatagga agtccgtgcg ttaacagcaa ctcactaagg aggtgatttt atgtatgtaa
     1021 ttggaattga tgtttccaag tacaaacacg actgtatcat catgcatgaa gatacaggtg
     1081 agatcgttag agatgtattc tctttcgata actcaagggc tggatttgtc cagtttataa
     1141 gtgtccttaa gactttaaat cctactttaa agaaaaagat aggatttgaa actactggcc
     1201 actaccagga taatcttaag atgttcctgg ctgacaacgg tcttgacttt atggaactta
     1261 atgccttact cgtccataag tttaagtctt cgctaacact cagaggcgta aagactgata
     1321 agaccgatgc aagattaatt gctaactatc tctgtaaagt agagttcaaa ccctatccaa
     1381 agatagtata caacattctt tcgcttcgtt cactatgtaa aagacatttt acgcttatca
     1441 aagaccgtac caagtattta ataagtatta ccaatttctt agaccgcact ttcccagaac
     1501 ttaagccatt ccttaacgat aatattaaga gtaagacggc gatgtttttg cttcgtaaat
     1561 acggcattcc tagcaaaatc gctaatatga atattcaatc ttatgaactt ttaagaaagc
     1621 tttcacgcgg gaagttttcg gtcgctaaat tctataaact taaggattta gctaaacata
     1681 caattggttt tacttatccc acagacacat ttgaaattaa gtcgctttta aatgtctatg
     1741 attcaataaa cgaagaaatt ttaaaagtag aaaccaaaat ttccactctt atgtcaaaga
     1801 tcaattccaa gatccctacc attaagggta ttggtcttgt ttccgcggca acaatcattg
     1861 ccgagattga ggatatcaat cgttttgttt cccctgataa actcttagct tacgctggac
     1921 ttgacccgcg gatttatgaa tccggcacta tgtcatctaa aggcaagatg aataagaagg
     1981 gctcagttaa tctccgtcgc gttcttatga actgtgctga gtcagtcata atgtttaatc
     2041 ccgctttcta cgcctactat cgtaagaaac gtgatgaagg taaggcgcat cgtgttgcta
     2101 tctcgcatct ggcaagaaag ctcgtacgca tcatctttaa gttagaaacc actcaagttg
     2161 atttcgatcc atcattagtt aagtaaggct aactattgag tgaggttttt taaaataatt
     2221 ctatgtttag aatatttttt actatgctct tatcatttaa cttgatttat taatagttaa
     2281 aacctcct
//
LOCUS       DAOSEX010000129         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_46945_length_2267_cov_3.068264, whole genome shotgun sequence.
ACCESSION   DAOSEX010000129 DAOSEX010000000
VERSION     DAOSEX010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46945_length_2267_cov_3.068264"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1020)
                     /locus_tag="PK612_02970"
     CDS             complement(76..1020)
                     /locus_tag="PK612_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95103.1"
                     /translation="MSVFIPLVPESNFQTSALGSYRNNPGDITQSYEVAIPFIASYTT
                     TLTYRYTVYNSANEIVYTQPSTSTRALRNVRVLITYRATAGKFTVGQNRIILYYKTSS
                     EAERRHYSYFYGYQKGVNVNLNNGSAARQLLEAEVCYLYDSTNRSDIFRKMSFSSANL
                     TPIKHFNHDLYFDFSGLFLSIGVLYDNENFYSSAALYCSNPALFPYLANVGGRAMQYL
                     NLTRAYNRLVTSVKTPLFVRADTLQIARSKYSAEYLNTDTYFFPKYHFDELQNTKFTL
                     EIKDFSYSKFNLKYEFTIQIDHPYFGTSGEHEVIINRY"
     gene            1145..1687
                     /locus_tag="PK612_02975"
     CDS             1145..1687
                     /locus_tag="PK612_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOH95104.1"
                     /translation="MIVLMGASASGKTEVAKMLGKLFSIKKVVTHTTREMRVNEVNGR
                     DYHFVTKEEFLRLKAEDYFVETVEYNDNFYGTSKAEIAMNKVLIIEPIGLKSIRALNS
                     EHIVVFFMQASRAIRRKRMINRGDNPKIATERILIDDAKFNPKNIEGIDYTIDSENQS
                     IKEIAIEVYEKYALKLVNFK"
     gene            complement(1680..>2267)
                     /locus_tag="PK612_02980"
     CDS             complement(1680..>2267)
                     /locus_tag="PK612_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95105.1"
                     /translation="PAKDAKTEDGVALTFGINYLGNFIFTQTLANHGLISAETRLIYI
                     SSPAYSRKFSPQILARAISGQKITRPKQYAVAKTAQKIFVLGLMTKSPLIPFENKGKI
                     YLYHPGIAVSNIARFKWKFFNKIVHRFMSVFFHPPEKAVLGALVALTTDKDLNGKILV
                     PRGFLEINGIPRIKKIDEKLLSHLPYLITETLKLI"
BASE COUNT          772 a          392 c          387 g          716 t
ORIGIN      
        1 gttcgttttg catactgtcc aaatactgaa ccggcaatta ataaaaataa aaaagcaaaa
       61 ttacgccatg ccatactagt acctattaat tattacttca tgttcaccgc ttgtgccaaa
      121 gtaagggtga tctatttgaa tggtaaattc gtattttaga ttaaatttac tataactaaa
      181 atctttgatc tctaatgtaa acttcgtgtt ttgtaactca tcaaaatgat acttaggaaa
      241 gaaatatgta tcggtattta gatattcggc actatattta ctacgcgcaa tttgtagtgt
      301 atctgctctc acaaacaatg gtgttttcac actcgttact aaacgattat aagcacgtgt
      361 taagtttaaa tactgcattg ctcttccgcc aacattagca agataaggaa agagagctgg
      421 gttgctacaa tataaagccg cactgctata aaaattttcg ttatcataaa gcaccccaat
      481 tgataaaaaa agcccactaa aatcaaaata taagtcatga ttaaaatgtt taataggcgt
      541 taaattagcg gagctaaaac tcatcttccg aaatatatca ctgcgattag tactatcata
      601 taaataacat acttcagctt ctaataattg ccgtgccgca ctaccattat ttaaattaac
      661 attgacacct ttttgatagc cataaaaata tgaataatga cgacgctctg cttcacttga
      721 tgttttgtaa tataaaataa tacgattttg accaaccgta aactttcctg ctgtagcacg
      781 atatgtgatt agcacccgaa cattacgcag tgcccttgtc gatgttgatg gttgagtata
      841 aacaatctca ttagcgctat tgtatacggt atagcggtat gttaatgttg tcgtgtaaga
      901 agcaataaaa ggaatcgcca cttcataact ctgtgtaata tctccaggat tattacgata
      961 actaccaagc gctgaagttt gaaaattact ttcaggtaca agcggaataa acacgctcat
     1021 aaaaatcgtt aaccacaatg ttttaaagta tctcatggta ataaataaaa gagtattttg
     1081 aactttttca catttacgac tgattcttcg tgcaattaac aaaattttat tttattattt
     1141 atgtatgatt gtactaatgg gagcaagcgc aagcggaaag acggaagttg ccaaaatgct
     1201 tggcaaactt tttagtatta aaaaagttgt gacgcacaca actcgtgaaa tgcgtgttaa
     1261 cgaagttaat ggtcgcgatt atcactttgt cacaaaagaa gaatttttac gacttaaagc
     1321 tgaagactac tttgtagaga ctgtagagta taatgataac ttctacggaa cttcaaaagc
     1381 tgaaatcgcc atgaataaag tattaattat tgaacctatt ggtttaaaat caataagagc
     1441 cttaaatagc gaacatatcg tcgtcttttt catgcaagct agtagagcta ttcgccgtaa
     1501 acggatgatt aatcgcggtg ataatccaaa aattgccact gaacgtattt taatcgatga
     1561 cgctaaattt aatccaaaaa acatcgaagg tatcgattat acgattgata gtgaaaatca
     1621 aagcataaaa gaaattgcga ttgaagtata tgaaaaatat gccttaaaac tcgttaattt
     1681 taaataagct ttaatgtttc tgttattaag taaggtagat gagataaaag tttctcgtct
     1741 atttttttaa tacgcggaat cccgttaatt tcaaggaacc cacgcggcac taaaattttc
     1801 ccatttaaat ctttatctgt cgttagcgcc acaagggcac caaggacggc tttttcggga
     1861 ggatgaaaaa acacactcat aaatctgtgg acaatcttgt taaagaattt ccatttgaag
     1921 cgtgcgatat tactaacagc tattcctggg tgataaagat aaattttacc tttattttca
     1981 aacggaatta gcggagactt agtcattaag ccaagcacaa atattttttg ggcagttttc
     2041 gcaacagcat attgtttcgg ccgcgtgatc ttttgtccgc ttattgcccg tgcaagtatt
     2101 tgcgggctaa acttacgcga atatgctgga ctagaaatat aaattaacct tgtttctgca
     2161 ctaattaacc cgtgatttgc aagtgtttgt gtaaaaataa agtttcctaa gtaattaata
     2221 ccgaatgtaa gcgcaacacc atcttcagtc ttagcatctt tagcagg
//
LOCUS       DAOSEX010000130         2265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_47011_length_2265_cov_2.820814, whole genome shotgun sequence.
ACCESSION   DAOSEX010000130 DAOSEX010000000
VERSION     DAOSEX010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47011_length_2265_cov_2.820814"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PK612_02985"
     CDS             complement(<1..446)
                     /locus_tag="PK612_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOH95106.1"
                     /translation="MKITILTLFPEMFTNFLETSIIKKARLQGHVTFDIVNIRDFADN
                     KWNRVDTPPVGGGAGMILQAEPIVKALESVDSGHKLLLSPRGNVLTNMKATALSQKEH
                     LIFICGHYEGIDERVNNFIDEEISIGDYILTGGELGAMVISDAAIR"
     gene            complement(443..958)
                     /gene="rimM"
                     /locus_tag="PK612_02990"
     CDS             complement(443..958)
                     /gene="rimM"
                     /locus_tag="PK612_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOH95107.1"
                     /translation="MNKDYLKIGYIVETHALKGALKIKPTTSFAEERFEVGNKVLVKN
                     NTTSKTETLTIQSVREQKGLLIVTFAEIDNINIAENYLKGFLFIAKKDITLEEGYFFF
                     DDLLGMKVVLEDETLVGEVIEILEYAAYHTLRVKREDGSNILIPYIEEFIVSTDLASK
                     TIVFRPIEGML"
     gene            complement(999..1253)
                     /locus_tag="PK612_02995"
     CDS             complement(999..1253)
                     /locus_tag="PK612_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOH95108.1"
                     /translation="MRDYEALVRTIITPLVKNEQALLIRQTNEGHRIIINIFAESDDI
                     AVLIGRRGVVANALREVVSVAGKLDEEHVFLRFEALDENK"
     gene            complement(1256..1531)
                     /gene="rpsP"
                     /locus_tag="PK612_03000"
     CDS             complement(1256..1531)
                     /gene="rpsP"
                     /locus_tag="PK612_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012094939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOH95109.1"
                     /translation="MAVKIRLTRVGRTHDPHYRIVAADSRYARDGRIIEQIGHYDPKK
                     DLKEAVIDRELALKWLGNGAQPSATVKALFRSQGINEEFAKSKKSGK"
     gene            1685..>2265
                     /locus_tag="PK612_03005"
     CDS             1685..>2265
                     /locus_tag="PK612_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOH95110.1"
                     /translation="MKNLLNKLFKFEERKANLTSEILGGLVTFLAMIYILPTNAGILS
                     DMGMDKSGVFVATAIVSAIVSLVMGLYGNFPIVLSAGMGVNAYLAYVVYGALGSWQAA
                     LLVMFFAGIIFAVISLSPLRKMMIDAIPNDIKYIISAGLGAFIAFVGFKSSGFIVASP
                     ATLVSLGNLRDPMVLLSLFGVLLVVIFMFSSNKR"
BASE COUNT          650 a          414 c          416 g          785 t
ORIGIN      
        1 aaacgaatag cagcgtcact aatgaccatc gcgccaagtt caccgccagt taagatgtag
       61 tcgccgattg atatttcttc atcgataaag ttattcactc tttcgtcaat tccttcataa
      121 tggccacaga taaagattaa atgttctttt tgacttagcg ctgttgcttt catattagta
      181 aggacattgc cgcgcggtga aagtaacagc ttatgaccag agtcaacact ttcaagtgct
      241 ttaacaattg gttcagcttg aagaatcatc cctgctccac caccgacagg tggagtatca
      301 actctattcc atttgttatc agcgaagtca cggatattaa caatatcaaa agttacatgg
      361 ccttgaagtc tcgctttttt aataattgat gtttcaagga aattggtaaa catttcggga
      421 aataatgtta atattgtaat cttcataaca taccctcaat tggtcgaaat actattgttt
      481 tgctcgctaa atcagtggaa acgatgaatt cttcgatata tggaattaga atattactac
      541 cgtcttccct ttttactctt aaagtgtggt aagcagcgta ttcaagtatt tcaataactt
      601 cacccacaag tgtttcatct tctaaaacga ctttcatccc taaaaggtca tcaaagaaaa
      661 agtaaccttc ttcaagggtg atgtctttct tagcgatgaa aagaaatcct tttagataat
      721 tctcggcaat attgatgtta tcaatttcgg caaaggtaac tattaatagt cctttttgtt
      781 cacgaacact ttgaatggtt aatgtttcag ttttagaagt agtattattt tttactaata
      841 ctttattacc aacttcaaaa cgttcctccg caaaagaagt agtcggttta attttaagtg
      901 caccctttaa ggcatgcgtt tcaacaatat aacctatttt taaataatct ttgttcataa
      961 ttttattata tcaatttaaa aaaactctcg agtgagagtt atttattttc gtctagtgct
     1021 tcaaaacgta agaagacatg ttcttcgtca agtttaccgg cgacagacac tacttcacgt
     1081 aatgcattag cgacaacacc gcgacgtcca attaagacgg caatatcatc actttcggca
     1141 aagatgttaa taataatacg atgtccttcg ttagtttgac gaataagtaa agcttgttcg
     1201 tttttcacta atggggtaat aatcgtgcgc actaatgctt cgtagtcacg cataattatt
     1261 tacctgactt ttttgatttt gcgaattctt cgttaatgcc ttgggagcgg aataaagctt
     1321 taacggttgc acttggttgt gccccatttc ctaaccattt aagcgctaat tcgcgatcga
     1381 taacggcttc tttaagatct ttctttggat catagtgtcc aatttgttcg ataatacggc
     1441 catcacgggc atagcgacta tcagcggcaa caatccgata atgtggatca tgagtgcggc
     1501 caacacgggt taatctaatt ttaactgcca tataagtaat cctccttttt ataccgtgat
     1561 aagtttagcg tgactcgttt acttagtcaa gtaattttac ttaactttag ttaaattagt
     1621 atgtatatct tttatttttc ttaaaattga ctaaaatagg tatattaaaa acaaggagca
     1681 atttatgaaa aatctactaa ataagctgtt caagtttgaa gaaagaaaag caaacttgac
     1741 aagtgaaatt ttaggaggat tagtcacctt tttagcgatg atttatattc tcccaacaaa
     1801 cgctggtatt ttaagtgata tgggcatgga caaaagcgga gtcttcgttg ctactgctat
     1861 cgttagtgct attgttagtt tagtgatggg actttatggt aatttcccga tcgtcctaag
     1921 tgctgggatg ggcgttaatg cttaccttgc gtatgtcgtt tacggagcac taggttcatg
     1981 gcaagcagcc ttgcttgtga tgttttttgc cggtattatt tttgcggtga ttagtctttc
     2041 accattacgg aagatgatga ttgatgcgat tcctaatgac atcaaatata ttatcagcgc
     2101 agggctaggt gccttcattg cttttgtcgg ctttaaaagc agtggtttta tcgttgctag
     2161 tcctgcgact ttagtgtcac ttgggaactt acgtgatcca atggtcttac tttctttatt
     2221 cggtgtttta cttgttgtta tcttcatgtt ttccagcaat aagcg
//
LOCUS       DAOSEX010000131         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_47159_length_2261_cov_2.379873, whole genome shotgun sequence.
ACCESSION   DAOSEX010000131 DAOSEX010000000
VERSION     DAOSEX010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47159_length_2261_cov_2.379873"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PK612_03010"
     CDS             complement(<1..880)
                     /locus_tag="PK612_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH95111.1"
                     /translation="MSDAYKDRVTIYEVAAAAGVSLATVSRVINNHPNVTPATRAKVQ
                     NAITRLGYRPSALAQALATSKTTNIGLIIPSANYVFISNMLHGIVMRANSLGYLITLF
                     VTGTEKEDALDAMDKLISSHVDGAIIFDDLFEEEEVTQITNYQVPVVVVNNRLDEDEK
                     VACITFGYENTLREDIIRHHLKTSDVMMHFVALNDNGRLLDRLQRSFVNTHKEENREY
                     KIHKVQDGYHAHYAYFKELFKTTKEGFFVAYRDSLGRAIVNAALEKGLRIPEDIEVIS
                     LIGTRYAVQGRPKLSSM"
     gene            complement(951..2051)
                     /locus_tag="PK612_03015"
     CDS             complement(951..2051)
                     /locus_tag="PK612_03015"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOH95112.1"
                     /translation="MTEIEMKNKDRKFRLTNKTFKFPSAIKDHFYTANYFVKTMEIAE
                     KHLADTVVTMQFFQRRDNVMACGVDESLALLQEFARYPEKLKIEACHDGDIVNANEPV
                     LKITGLYKDFAYLESLIDGILTRRTTAATNSYEVLQAAYPTEVFSMADRQDDYLTQAG
                     DGYASYIAGIKKFSTDAQGAWVNVKGMGTMPHALIAMTNGDLIKACELYHKTFPNEKI
                     SALVDYHNDTVTEALKVARHFGAKLGSIRLDNSKALLDEYFVRQGLNDPRLKGVSPEL
                     VFAVREALDKEGFNYVNIVVSSGFTPEKIKYFKAKNAPVNLYGVGSFIVNNKEVGFTG
                     DVVVVNGKPQAKVGRQEYFSDKLEKVTLKKRM"
     gene            complement(2056..>2261)
                     /locus_tag="PK612_03020"
     CDS             complement(2056..>2261)
                     /locus_tag="PK612_03020"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HOH95113.1"
                     /translation="PGGTDAGAIHLHAVGVLTLTHCVCARSIHTSASVVDTTDFKNAK
                     KVLIRMLRTLNRDKINKFKEARR"
BASE COUNT          670 a          491 c          364 g          736 t
ORIGIN      
        1 tcatacttga taattttggg cggccttgca cagcatagcg tgtcccaatt aaactaatta
       61 cttcaatgtc ttctggaatg cgtaagcctt tttcaagcgc ggcattaaca atcgcgcgtc
      121 ctaatgagtc acgataagca acaaagaacc cttcttttgt cgtcttaaat agttctttga
      181 agtaagcata atgcgcgtga tatccgtctt gtactttgtg aatcttatat tcacgatttt
      241 cttctttatg cgtgttaaca aagcttcttt gtaaacgatc taacaaacga ccattatcat
      301 taagtgctac aaaatgcatc atcacatcac ttgtttttag gtgatgacga ataatatctt
      361 cacgtaatgt attttcataa ccaaaagtaa tacatgcaac tttttcatct tcatcaagac
      421 gattattaac gacaacaaca ggaacttgat aattcgtaat ttgcgttact tcttcctctt
      481 caaataagtc atcaaagata attgccccat caacatgcga actaattaat ttatccatag
      541 cgtcaagtgc atcttctttt tctgtaccag tgacaaataa agtaattaga taccctaaac
      601 tattcgcacg catcacgatg ccgtggagca tatttgaaat aaatacatag ttggcactag
      661 gaataattaa gccaatattc gtcgttttac tcgtggcaag cgcttgcgct aaagcacttg
      721 gacgataacc aaggcgcgta atggcgtttt ggactttagc ccgcgtcgct ggtgtgacat
      781 ttggatgatt attaatgaca cgcgacacag ttgctaggct aacgccagcc gctgctgcta
      841 cttcgtagat tgttactctg tctttatatg catcactcat atttaaaagc cttctttcat
      901 accaatttta cttgaaaata ttttcattgt caatgtagaa gagaaagccc ttacattcgc
      961 tttttaagtg ttactttttc aagcttatca ctgaagtatt cttgccgtcc aacttttgcc
     1021 tgcggtttgc cgttaacaac gacaacatca ccagtaaatc cgacctcttt attgttaaca
     1081 ataaaagagc ctacaccata gagattaaca ggagcatttt tggctttaaa atatttgatt
     1141 ttttcaggag taaagccaga acttacgacg atatttacat aattaaaacc ttctttatca
     1201 agtgcttcac gcacggcgaa gactagttca ggactaacac cttttagcct tggatcattt
     1261 aaaccttgac gcacaaaata ttcatcaagt aatgctttac tattatcaag acgaatcgag
     1321 cctaatttcg caccaaaatg acgcgctact tttaaggcct ctgtcactgt atcattatga
     1381 tagtcaacaa gcgcactgat cttttcattt gggaaagttt tgtgataaag ttcacaagcc
     1441 ttaattaagt caccattagt catcgcaatt aaagcatgtg gcattgttcc catcccttta
     1501 acatttaccc acgcaccttg cgcatcagtg gaaaactttt taatccccgc aatataacta
     1561 gcgtagccat ctcccgcctg tgttaaataa tcatcttggc ggtctgccat actaaaaact
     1621 tcagttggat aagcagcttg caacacttca taactattgg ttgctgctgt cgttctccgc
     1681 gttaaaatgc catcaataag actttctaaa tacgcaaaat ctttgtataa acctgtaatt
     1741 tttaacactg gttcattcgc gttaacaata tcaccatcat gacacgcttc aatctttaac
     1801 ttttcggggt aacgcgcaaa ttcttggagt aaggctagtg attcatctac accacacgcc
     1861 atcacattat cccgccgttg aaaaaattgc atcgtcacga ctgtgtccgc taaatgtttt
     1921 tcagcaattt ccatcgtttt tacaaagtaa ttcgcggtat aaaaatggtc tttaatcgca
     1981 cttggaaatt taaaggtttt gtttgttaaa cgaaacttcc gatctttgtt tttcatttca
     2041 atttctgtca ttttcttatc tcctagcttc tttaaattta ttaatcttat cgcgatttaa
     2101 tgttcttaac atgcgaatta atactttttt agcattttta aaatcagtcg tatcaacaac
     2161 actagcggaa gtgtgaatag agcgcgcaca aacacagtgc gtcagtgtta aaacaccaac
     2221 agcgtgtaaa tgaattgctc cagcatcagt cccacctgga c
//
LOCUS       DAOSEX010000132         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_47210_length_2259_cov_3.343920, whole genome shotgun sequence.
ACCESSION   DAOSEX010000132 DAOSEX010000000
VERSION     DAOSEX010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47210_length_2259_cov_3.343920"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PK612_03025"
     CDS             <1..844
                     /locus_tag="PK612_03025"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779933.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOH95114.1"
                     /translation="QENDFYEIFKVMKGLPVTVRLLDPPLHEFLPTEEAQIVALAKEL
                     KVDVTVLRERIESLSEFNPMMGHRGVRLAVSYPEIAVMQTTAIISAALRAQKDTGAAI
                     HPEIMIPLVLDVKEYKYVEEYVRKTADALIKKSGQKLHYLVGTMIEIPRATLLADQIA
                     KEAEFFSFGTNDLTQMTFGFSRDDAAKFIPDYTEARIFEQDPFQTLDTEGVGQLVRLG
                     VERGRKGNPDVHLGICGEHGGDPNSVEFFDSVGLDYVSCSPYRVPIARLAAAQAAIKH
                     KKAK"
     gene            complement(949..>2259)
                     /gene="glmU"
                     /locus_tag="PK612_03030"
     CDS             complement(949..>2259)
                     /gene="glmU"
                     /locus_tag="PK612_03030"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOH95115.1"
                     /translation="GFEILGKPLINWVLDAAKPHVTGRTVVIVGFGGEHTSKLVEGKA
                     EIAWQHEQKGTGHAIIQTAPLLEKEKGDTLILSGDVPLITEHSISGLLNLHTKEKNDL
                     TILTAKPDNPFGYGRVLRAPNGEVTRIVEQKDLTGNEGDISEVNAGMYVVNNQKLFQE
                     LKNLKPNNKQGELYLTDIIGLFKERGYKVGAFVLSDPDEMLGTNDRAQLAEAAELLKK
                     RINKAHMLNGVTLEDPNNTYIGPDVTIGQDTIISPNTTILGNSTIGVANEIGPNTYLK
                     NMKIGDYNSIIYSYLVDSEICNNVTIGPFARMRQKALVKDGSHIGNFVELKNTTFHEG
                     VKAGHLAYMGDAELGAHTNVGAGAITANYDGVNKSKTKVGENVFIGTNTTLVAPLQIG
                     EGSYLAAASIINKDVPKDTLAIARSRQENKEGYAKIIREKQKKK"
BASE COUNT          617 a          474 c          422 g          746 t
ORIGIN      
        1 ccaagaaaat gacttctatg aaatcttcaa ggtgatgaag ggattaccag tcactgttcg
       61 tttacttgac ccaccgttac acgaattctt accaaccgaa gaagcacaaa tcgttgcttt
      121 agccaaagaa cttaaagttg atgtcactgt tcttcgcgaa cggattgaat cgctttcaga
      181 atttaaccca atgatgggtc accgtggtgt gcgtttagct gtttcttacc cagaaatcgc
      241 tgtgatgcaa acaactgcta ttattagtgc cgccttacgt gcacaaaaag acactggtgc
      301 cgctatccac ccagaaatca tgattccact agtgctagac gttaaagaat ataaatacgt
      361 tgaagaatat gtacgtaaaa ccgctgatgc attaattaag aagagtgggc aaaaacttca
      421 ttacctcgtt ggtacaatga ttgaaatccc acgagcaacc ttacttgccg accaaattgc
      481 taaagaagcc gaattcttca gctttggaac aaacgactta acgcaaatga catttggctt
      541 tagccgtgat gatgctgcga agtttattcc tgactatact gaagcacgga tcttcgaaca
      601 agacccattc caaaccttag acactgaagg cgttggccaa ttagtgcgct taggtgttga
      661 acgcggtcgt aagggcaacc ctgatgttca cttaggaatc tgtggtgagc atggtggtga
      721 tccaaactct gtcgaattct ttgatagcgt tggcttagac tatgtcagct gttcaccata
      781 tcgtgtgcca attgctcgct tagccgcagc ccaagctgca atcaaacata aaaaagctaa
      841 gtaataatta ctattaagat agctttaacc tcgttcttcg gagcgaggtt ttttattatt
      901 taaaaagacg ctcaatacaa gcgtctttcg ttaatttctc agcaatatct atttcttttt
      961 ttgtttttca cgaattattt tcgcatagcc ttctttattt tcttggcgtg aacgcgcgat
     1021 agcaagtgtg tcttttggta catctttatt aatgatcgat gcagcggcaa gataagagcc
     1081 ttccccaatt tgaaggggtg ctactagggt cgtgtttgtg ccaataaaga cattttcacc
     1141 cacttttgtt tttgatttat ttacgccatc ataatttgca gtaattgctc ctgcaccaac
     1201 attagtgtgt gcgcctaatt cagcatctcc catatacgct aagtggccag ctttaacgcc
     1261 ttcgtggaat gttgtatttt taagttcgac aaagttccca atatgtgagc cgtcttttac
     1321 caaagctttt tgtcgcattc tcgcaaacgg accaatcgtg acattgttac aaatttctga
     1381 gtctactaag taactataaa taatagaatt ataatcgcct atcttcatat tctttaaata
     1441 tgtgtttggt ccaatttcat ttgcaacacc aatcgtgcta ttgcctaata tcgtagtgtt
     1501 aggactaata attgtatctt ggccaattgt gacgtctggt cctatatatg tgttatttgg
     1561 atcttcaagt gtaacgccgt ttagcatatg tgctttatta atgcgttttt taaggagttc
     1621 cgcagcttcg gcaagttgcg ctctatcatt tgtgccaagc atttcatcag gatcacttaa
     1681 cacaaaggca ccaactttat accctctttc tttaaataaa ccgataatat ctgttaaata
     1741 taattcgcct tgtttattgt ttggttttaa gtttttaagt tcttggaata acttttggtt
     1801 attaacgaca tacatcccag cattaacttc actaatgtcg ccttcgttac ctgttaagtc
     1861 tttttgttca acaatgcgag ttacttcacc gtttggtgcg cgtaaaactc taccataacc
     1921 aaaaggatta tctggcttag cggttaatat tgttaaatcg tttttctctt tggtgtgaag
     1981 attaagtagt ccgctgatgg aatgttcagt aataagtggt acgtctccac ttaaaattaa
     2041 agtgtcaccc ttttcctttt caagtagcgg tgctgtttga atgattgcgt gtcctgtacc
     2101 tttttgttca tgttgccaag caatttcagc tttcccttcc acgagtttag aagtgtgttc
     2161 accaccaaaa ccgacaatca caacagttct tcctgttaca tgcggctttg cggcatctaa
     2221 cacccaatta ataagtggct tgcctaatat ttcaaaacc
//
LOCUS       DAOSEX010000133         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_47952_length_2235_cov_3.577064, whole genome shotgun sequence.
ACCESSION   DAOSEX010000133 DAOSEX010000000
VERSION     DAOSEX010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47952_length_2235_cov_3.577064"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PK612_03035"
     CDS             complement(<1..285)
                     /locus_tag="PK612_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95116.1"
                     /translation="MAKRRESKTRVEYWAKYRESITNDFEVEQKMRESRKKQAENTPI
                     KKTETPPLTKAEALLSEYERKNENKESAVKKGPIGILYGLAIIVLLLIIAG"
     gene            410..604
                     /locus_tag="PK612_03040"
     CDS             410..604
                     /locus_tag="PK612_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOH95117.1"
                     /translation="MAKKVKLDKDLCISCGVCNAICPSLFDWDDDGKLKALVEEVPAD
                     LEDAAAEAEASCPTGAITVE"
     gene            complement(659..1318)
                     /locus_tag="PK612_03045"
     CDS             complement(659..1318)
                     /locus_tag="PK612_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOH95118.1"
                     /translation="MEKEKNYTNCQNDNLETNAQQKCKRRRTVTVAGTAIFAAISVLL
                     YITKVPPFVFPIFPLVNFLEINFSEIPALIAGFAYGPLSGFLVLLVRFLVKLPMSSTA
                     MVGEVADIIYSSSFVLTATIIYKYRRTFKGALLGLGIAFIAQILVSSLANYFVIYHLY
                     NEMFFNGKMAMILSNKYGAFTPEQFILWVIPFNIIKNTIIGGLTLVVYKRISRFINQF
                     Y"
     regulatory      complement(1357..1469)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1509..>2235)
                     /locus_tag="PK612_03050"
     CDS             complement(1509..>2235)
                     /locus_tag="PK612_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOH95119.1"
                     /translation="EELAKAHTNRPIQMLPIDLIVDNNFIKKITLSRKKIARFVENYQ
                     RNILLEPLVVRAKGDKYEVIIGRRRLAAARLTEIQEIPVIVMNYNDEETLLILLAKAR
                     DQLGTNVLELAYIFSVLSTKHGYSHQALADLTFRSRSQVTNILRLLKLEPEVIRALNN
                     EQITFGHARALISFDREKSLEYLELIRVNNLSVRELEALIKAPNTATYNGKDYFVIQM
                     KNKLTIVFNTEEEALAFKNKHFK"
BASE COUNT          706 a          412 c          372 g          745 t
ORIGIN      
        1 accagcaata ataaggagca atacgataat agctagccca tataatatgc ctattggtcc
       61 tttttttact gcgctttctt tattttcatt tttcctttca tattcactaa gtaaagcttc
      121 agcctttgtc agcggtggtg tttcagtctt tttaatagga gtattttcag cttgtttttt
      181 ccgtgattca cgcatttttt gttcaacttc aaaatcattc gtaatactct cacgatattt
      241 tgcccagtac tctactctcg ttttactttc tctacgcttt gccatactgt atttataata
      301 atataaataa acataaaatg cttacacgac aattttataa ttatcgtttc caatatgatt
      361 tgaattgata ctctcaaata gttataatat tatccatcag gaggaaatta tggcaaaaaa
      421 agttaaactc gataaagatt tatgtattag ttgtggcgtt tgtaacgcaa tttgtccttc
      481 ccttttcgat tgggatgatg acggaaaact caaagctctc gttgaagaag ttccagcaga
      541 tttagaagat gctgcagctg aagccgaagc ttcgtgtcca actggtgcaa tcaccgtcga
      601 ataagttaag agaattaaaa aaataccagc agccacttcg ggtgctggtt tttttatgct
      661 aataaaactg atttataaag cgactaattc gtttatatac aacgagtgtt agtccgccga
      721 taattgtatt tttaataata ttaaaaggga tgacccataa aataaattgc tctggtgtaa
      781 aagccccata tttattactt aaaatcattg ccattttacc gttaaaaaac atttcattgt
      841 aaagatgata aataacgaag taattggcaa gactgctaac taaaatttgc gcaataaagg
      901 cgatgcctaa accaagaagc gcccctttga aagtccgccg atatttatat atgattgtgg
      961 cagtaaggac aaagctcgat gaatatataa tatcagcgac ttcaccgacc attgcagttg
     1021 atgacatcgg tagttttact agaaacctca ctaataacac aaggaatccg cttaaaggcc
     1081 cgtaagcaaa gccggcaata agtgcaggaa tttcactaaa attaatttct aaaaaattaa
     1141 caagcgggaa aattgggaac acaaacgggg gaactttagt aatataaagt aagactgaaa
     1201 tagcggcaaa tattgccgta ccagcgacag taactgttct tctgcgttta catttttgtt
     1261 gtgcattagt ttctaaatta tcattttgac aattggtgta atttttctct ttttccataa
     1321 aaaatagccc tctctattca gggctacaca taaaagaaac aacttcttcc atccagactt
     1381 taactgtcgc cactagaatt tcactagttc agcttgcgct cgcggggtat accgccggtg
     1441 gggactttca ccccgccctg aagtactttt ataatagtag ttatcggtaa taaattcaag
     1501 ataaatgatt atttaaaatg tttgttttta aacgctagtg cttcttcttc ggtattaaac
     1561 acaattgtta atttattttt catttgaata acaaaataat ccttaccatt atatgtcgca
     1621 gtatttggtg cttttattaa ggcttctaat tcacggacag agagattatt aacacgaatt
     1681 aattctaaat attcaagact tttttcgcga tcaaatgaga ttaaagcccg tgcatgtcca
     1741 aaagttattt gctcgttatt aagcgcacga ataacttctg gctcaagctt taaaagtcgt
     1801 aaaatatttg taacctgcga cctgcttctg aatgttaaat ccgcaagtgc ttggtgagaa
     1861 tagccgtgtt ttgtcgataa aacactaaag atatacgcta attctaaaac attagtacca
     1921 agttgatcac gcgctttcgc caataaaatt aataaggttt cttcatcatt ataattcata
     1981 acgatgacgg gaatttcttg gatttctgta agtcttgctg ccgctagacg ccggcgtcca
     2041 ataattactt cgtacttatc accttttgct ctaaccacga gtggttctag taaaatatta
     2101 cgctgataat tttcaacaaa gcgcgcaatt tttttacggg acaaagtaat ctttttaata
     2161 aaattattgt ctactattaa atcaatcggc aacatttgaa tcggccgatt cgtatgtgct
     2221 ttcgcaagtt cttct
//
LOCUS       DAOSEX010000134         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_48863_length_2207_cov_3.646375, whole genome shotgun sequence.
ACCESSION   DAOSEX010000134 DAOSEX010000000
VERSION     DAOSEX010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48863_length_2207_cov_3.646375"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1118)
                     /locus_tag="PK612_03055"
     CDS             complement(<1..1118)
                     /locus_tag="PK612_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95120.1"
                     /translation="MKIKTRLDLIVIDGTELVVNEALKTLAKLPVSDLVEFLKGPKLY
                     SRRDHANILKNVMRDEVWKFKLVSRVAEKGLSNDFFSRLDYFEDSPVTVLEGLFERMV
                     KIQPELMDYYLEDLWLFAIQVVSKDKELAKLYKKAQKSSTSPVVLKELFPTLEGLFGD
                     EEGDIDGVLARTFVEKSLHSATIKEIREIAEKNDVLLPTNITKQDIVDGIIAVLPKVA
                     KAKDVPHLTEDVKDMTVAQLKEFVNKHKLDVPTELKKQDVGELLVKNYVNKNAANVVS
                     DLQYELPERVEEATDFSDENPKVIRLRERVKELEKELEERPVENETKVDESELLKEQE
                     KVEALSKELKQTKAELSEKPKEVVVEVPGPTVIRTVIS"
     gene            complement(1286..>2207)
                     /locus_tag="PK612_03060"
     CDS             complement(1286..>2207)
                     /locus_tag="PK612_03060"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOH95121.1"
                     /translation="AIVDGSMLNQFSMDYYNGHLRVATTNIGVSWLFDEENYISTWSQ
                     VVINHLYVLKVNKRTKTFTLVGHLSEGLGKPNETIKSVRFLGDKAYIVTFETRDPLYI
                     IDLSNPAKPVITGDIELPGYDVYQHPFGDSFVLGLGYSADDDGFTSGIKLTAYATSSG
                     NEHEIQTYIVSTYRYESAENYWSYSYGFTEALWNHKAMLISVKDGVFAFPYFEYGFSC
                     SYERDGDSYVSSDCQQSFSFNYQIYNIDFSRETPIAEPIKISHPAVEEYVYPINRSVL
                     IEGVLYIFSTYHVSTYDLATKISTAPVYIA"
BASE COUNT          650 a          429 c          362 g          766 t
ORIGIN      
        1 ttgctaatta ctgtccgaat tactgttggt cctggcactt caacgacgac ttcttttggt
       61 ttttctgaaa gttcagcctt tgtttgcttt aattctttag aaagtgcttc aactttttct
      121 tgttctttta aaagttcgct ttcatcaacc tttgtttcat tttcgacagg tctttcttct
      181 aattctttct cgagttcctt aacgcgttcg cgcaacctga taacttttgg attttcatca
      241 ctaaaatcag tagcttcttc gacacgttct ggtaattcat attgtaaatc actaacgaca
      301 tttgccgcat ttttgtttac atagttttta acgagtaatt caccaacatc ttgtttttta
      361 agttcagttg gaacatcaag tttatgcttg ttcacaaatt ctttcagttg tgcaacagtc
      421 atatccttaa catcttccgt taaatgcggg acatcttttg ctttcgctac ttttggtaaa
      481 acagcaataa ttccgtccac tatgtcctgt tttgtgatat ttgttggtag taaaacatca
      541 tttttttcgg caatttcacg gatttcttta atcgtggcac tgtgtaagct cttttccaca
      601 aatgtgcgcg ctaatacacc gtcgatatcg ccttcttcat caccgaaaag cccttcaagt
      661 gtcgggaata attccttaag aacaactggg cttgtgctac ttttttgtgc tttcttataa
      721 agcttagcaa gttctttgtc cttgcttaca acttgaatgg caaagagcca taagtcttca
      781 agataataat ccattagttc gggttgaatc ttgaccatcc gttcaaataa tccttcaaga
      841 acggtgacag ggctatcttc aaaataatca agtcgactaa agaaatcatt tgaaagtcct
      901 ttctccgcaa cgcgtgagac aagtttaaac ttccagactt catcacgcat aacattcttt
      961 aaaatgtttg catgatctcg acgtgaatat aatttagggc cctttaaaaa ttcgactaag
     1021 tcgcttactg gtaattttgc cagtgttttg agagcttcat taacaacgag ctcggttcca
     1081 tcaattacga tgagatctaa tctggtttta attttcatgt tgttttcccc tttgctaaca
     1141 ttatatcatt gaaaagtgat gatgagtcaa tgagttgaaa tttttttctc atttttttga
     1201 atttaggcgt gttttgtcgt taaaaatgaa caaaaagcac tacttactta gtgattttcg
     1261 tggtaaatag tgcttataaa attagttaag cgatgtagac tggagcagtg ctaatctttg
     1321 tcgctaagtc gtaagtacta acgtgataag ttgaaaaaat atagagcact ccttcaatta
     1381 acacggaacg attaatcggg taaacatatt cttcaactgc tgggtgactt attttaatgg
     1441 gttccgctat tggagtttca cgactaaagt caatattata aatttgataa ttgaaagaaa
     1501 atgattgctg acaatcactt gatacatagc tatccccatc ccgctcatat gaacaagaga
     1561 aaccatattc aaaatacggg aaagcaaaca cgccgtcttt tactgaaatt agcattgctt
     1621 tatggttcca aagtgcttcg gtaaaaccat acgaatatga ccaataattt tcagcacttt
     1681 cgtaacgata agtacttaca atatatgttt gaatttcgtg ttcattacca gaactagtag
     1741 cgtatgcagt taatttaatg ccgctcgtaa aaccgtcatc atcagcgcta taacctagtc
     1801 ctaacacaaa actatcacca aatggatgtt gatatacatc ataacctggt aattcaatgt
     1861 caccagtaat aactggctta gcaggattac ttaaatctat aatataaagt ggatcacgtg
     1921 tttcaaaagt aacaatataa gctttatcac ctaaaaagcg cacggactta attgtttcat
     1981 tgggtttacc taaaccttca ctcaagtgtc ccactaatgt gaatgtcttc gttcttttat
     2041 taactttaag tacataaagg tgattaataa ctacttgtga ccatgtacta atataatttt
     2101 cttcgtcaaa tagccagctt acgccaatat tcgtcgtagc gactcggaga tgcccgttat
     2161 agtaatccat agaaaattga ttaagcatcg agccatcaac aattgcg
//
LOCUS       DAOSEX010000135         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_49410_length_2191_cov_2.812266, whole genome shotgun sequence.
ACCESSION   DAOSEX010000135 DAOSEX010000000
VERSION     DAOSEX010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49410_length_2191_cov_2.812266"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PK612_03065"
     CDS             complement(<1..695)
                     /locus_tag="PK612_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH95122.1"
                     /translation="MELKDTLLMPKTKFEMRGNLTRKEPGILARWEAIDLYKLMVKKN
                     ETSKPFILHDGPPYANGNIHSGHMLNRNLKDFVVRMKNMQGYDTPFIFGWDTHGLPIE
                     NQVTKSGVNRKAIPITEFRKLCKEYALKQVEIQKAQLKRLGLVGDFENAYLTLTKDYE
                     ADELRVFKRMALKGLIYRGLKPVQWSPSSETALAEAEIEYKDVVSDSIYVLFNVTKGN
                     DKVPVGTNFMIWTT"
     gene            complement(966..2060)
                     /gene="ftsZ"
                     /locus_tag="PK612_03070"
     CDS             complement(966..2060)
                     /gene="ftsZ"
                     /locus_tag="PK612_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOH95123.1"
                     /translation="MIGTNDLDNFEPVARIVVIGVGGAGNNAVNRMIDDQIDSVSFWV
                     ANTDKQALATSKAKNRIVLGEEFTDGLGAGGEPSVGRKAAEASRPEIEKIVEGADLVF
                     IAAGMGGGTGTGASPIIAEIAKAAGALTIAIVTRPFTFEGKKRIANSVEGLNALKEIV
                     DSIIVVSNDKLLITGGNLPIAQAFSESDKVLAQSVKTVVDLILVPAVINLDFADVRNT
                     LLNSGIALIGFGMGKGKNKAHDAAQTAINSPLLEASLQGARKAIIAVTCGTNVSLFEA
                     QETVNEIVETSGSNLDVKFGVALNELLEDSIIVSVIASDFEEQYDFTSTHTSIIKPVT
                     PESRTYHAEDDEDDDSFLPNILKELEAEEN"
BASE COUNT          601 a          487 c          389 g          714 t
ORIGIN      
        1 gttgtccaaa tcataaaatt agtgccaaca ggtactttat cattgccttt tgttacatta
       61 aaaagcacat aaattgagtc actaacgaca tctttatatt caatttcggc ttcagcaagt
      121 gcagtttcac tacttggtga ccattgcacg ggctttagtc cgcgataaat taaaccttta
      181 agtgccatac gcttaaagac acgaagttca tcagcctcgt aatctttagt caaagttaaa
      241 taagcatttt caaagtcacc gactaagcca agacgcttaa gttgagcttt ttgaatttcg
      301 acttgcttaa gtgcatattc tttacataac ttgcggaatt ctgtaattgg aattgcttta
      361 cggttaaccc cactttttgt gacttggttt tcaattggga gaccgtgtgt atcccagcca
      421 aaaataaagg gcgtgtcata gccttgcata tttttcatgc gtacgacaaa gtcttttaag
      481 ttacggttaa gcatatgtcc gctatggatg ttcccgttag cgtaaggtgg cccgtcatgg
      541 agaataaaag gtttacttgt ttcatttttc ttcaccatta atttatataa atcgattgcc
      601 tcccaacgcg ctaaaatacc tggttccttg cgggttaagt taccccgcat ttcaaacttt
      661 gtctttggca ttaataatgt gtctttaagt tccatagtct ctctccttca ataaaaaaag
      721 tctagtcaca ggacgaatta ctccgcggta ccacctgtgt tctagactaa tctagcactt
      781 tgtatctcgt aacgtgagat taagcgacaa ctattactct tgttcatagt tgcactcaga
      841 agtgataatg ggagtagctt cttcttaggg cttgcaccat ttccctagtc gctttgtaag
      901 gttctaccgc caccgtgtct tcgtccgatt tcgctactat tatacattaa agtataataa
      961 tgtcattaat tttcttctgc ttctaactct tttaagatat ttggtaagaa gctatcatca
     1021 tcttcatcgt cttcagcgtg ataagtacgc gattctggtg tcactggttt aataattgaa
     1081 gtgtgcgtac tagtaaagtc atattgctct tcaaagtcac tagcaatgac actgacgata
     1141 atggagtctt caagtaattc atttaatgcg acaccaaatt taacatcaag atttgatccg
     1201 cttgtttcaa cgatttcatt aactgtttct tgtgcttcaa aaagtgaaac attcgtcccg
     1261 catgtgacag caataattgc ttttcttgcc ccttggagac ttgcttctaa aagcggacta
     1321 ttaattgctg tttgtgctgc gtcgtgcgct ttatttttcc ctttccccat cccaaagcca
     1381 ataagggcaa taccgctatt taagagggta ttacggacat cggcaaagtc aagattaatc
     1441 acagccggta ctaaaattaa gtcgacaact gtttttacac tttgcgctaa tactttatca
     1501 ctttcactaa aagcttgagc aatcgggaga ttaccaccag tgataagtaa tttatcatta
     1561 ctaacaacga taatggagtc aacaatttct tttaaggcat taagtccttc aacactgtta
     1621 gcgatacgtt ttttaccttc aaaggtaaag ggccgcgtaa caatcgcgat tgttaaagca
     1681 ccagcggctt tcgcaatttc agcaatgatg ggggaggcac ctgtacctgt tccaccaccc
     1741 atacctgcag cgataaagac aaggtcagca ccttcaacga ttttttcaat ttcgggacga
     1801 ctagcttcgg cggctttccg cccaacactt ggttcaccgc ccgcacctaa accgtctgta
     1861 aattcttcac caagcacaat acgatttttc gctttacttg tggcaagtgc ttgtttatcg
     1921 gtatttgcca cccagaaaga tacgctatcg atttgatcat cgatcatgcg atttacggca
     1981 ttattaccag cgccaccgac accaataact actattctag ctacaggttc aaaattgtct
     2041 aaatcattcg ttccaatcat agttaactcc ttgttagtgt cccaatactt gctttctcgt
     2101 ccccgatatc ttgggcatac ttactataag ctttaatgag tcctaaatta acagcgtaag
     2161 ttaagtctct tgccccaatg gtatcgagtt t
//
LOCUS       DAOSEX010000136         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_49467_length_2189_cov_3.132146, whole genome shotgun sequence.
ACCESSION   DAOSEX010000136 DAOSEX010000000
VERSION     DAOSEX010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49467_length_2189_cov_3.132146"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PK612_03075"
     CDS             <1..199
                     /locus_tag="PK612_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HOH95124.1"
                     /translation="GAYNGIIGADRERVIERTWFDKQVPFTYEYKDKEAQFSAVILEI
                     NKHTFKTEKITPLYYLWRRDE"
     gene            192..1628
                     /gene="miaB"
                     /locus_tag="PK612_03080"
     CDS             192..1628
                     /gene="miaB"
                     /locus_tag="PK612_03080"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006505481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOH95125.1"
                     /translation="MSKTLLNNLPSFTKARRRYPSIVQNLKIEVPSIVSAKLSGKFFL
                     IKTFGCQANVRDEESMAGLLSMANMRRTEEEKDASVIIINTCAVREGAEQKIFGQVGN
                     LKGLKTNNKDLIVIVAGCVVQQEESLAKLRKTFPYVDIFMGTNAIHKLLDLIALRITE
                     NKKVVDVPSDDADVYENIPVQRIAPHKAFVNITYGCDKFCTYCIVPYTRGKERSRLPA
                     DILRECQILVDSGYQEITLVGQNVNAYGIDLKDGTTFSYILEEVAKLGIPRLRFITSH
                     PWNFDDEMIDIIAKYPNIMRKIHLPLQSGSDEILKLMGRRYTVKDYLELVTKLRARMP
                     DISLTTDIIVGFPNETEQQFLETLKVVEKVNYENAYTFIYSPRKGTPAALMEDNVSRE
                     EKGERFNKLKALVDSQTEANAALYVGNEYLILVDGYSKKDTSVLSGYTESDRVVHFVG
                     TPDLIGKIVKVKITESRVYSFVGELVNE"
     gene            1621..1932
                     /locus_tag="PK612_03085"
     CDS             1621..1932
                     /locus_tag="PK612_03085"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HOH95126.1"
                     /translation="MNKVEIALNALTTELANDKRVVEFKKVKALIESDAYLKNAEARL
                     KELQRLMTQNAFNEEKHNEYKREYLRLKNNYETHPYLINYNSLLSEIEDLLYSLKTVI
                     E"
     gene            1985..>2189
                     /locus_tag="PK612_03090"
     CDS             1985..>2189
                     /locus_tag="PK612_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008816030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95127.1"
                     /translation="MVYTPMIEQYLEIKRENPGILIMYRLGDFYEFFFEDAEVVSKEL
                     QLVLTKRAAGNNRKIPMCGIPHHA"
BASE COUNT          784 a          324 c          425 g          656 t
ORIGIN      
        1 gggggcatat aatggcatca ttggtgcaga tagagaacgc gtaatcgagc ggacttggtt
       61 tgataaacaa gtaccgttta cttatgagta caaagataaa gaagcgcaat ttagcgctgt
      121 cattttagaa attaataaac atacatttaa aacagaaaaa ataacaccac tttattactt
      181 atggagaaga gatgagtaaa acattactta ataatttacc ctccttcact aaagcacgaa
      241 gacgttatcc gtccatagtt caaaatctta agattgaagt accgtcaatt gttagtgcga
      301 agttaagtgg taaatttttc ttaattaaaa catttggctg tcaggcaaat gtacgcgacg
      361 aagaaagtat ggccggtctt ttaagtatgg ctaatatgcg acgcactgaa gaagaaaaag
      421 acgctagtgt aattattatt aatacatgtg ctgttcgtga aggggctgaa caaaaaatct
      481 ttggtcaagt cggtaactta aaaggactaa aaacaaataa taaagattta atagtaattg
      541 ttgcgggttg tgttgtgcaa caagaagaat cactcgcgaa attacgaaaa acattcccat
      601 atgttgatat ttttatgggg acaaacgcta ttcataaatt acttgattta attgccttac
      661 gaataacgga aaataaaaaa gtagtggatg tgcctagtga tgatgccgat gtctatgaaa
      721 acatccctgt gcaacgcatt gccccgcata aagcttttgt caatataaca tatggttgtg
      781 ataagttttg tacatattgt atcgtccctt acactagggg gaaagaacgc tcacgattac
      841 ctgctgatat tttacgagaa tgtcaaattt tagtggattc tggttatcaa gaaattacac
      901 tcgttggtca aaatgttaat gcttatggta ttgatttaaa agatggcaca acttttagtt
      961 atattttaga agaagtagca aaacttggca tcccacgcct tcgttttatt acttcgcacc
     1021 catggaactt tgatgatgaa atgattgata ttatcgctaa atatcctaat atcatgcgta
     1081 aaattcattt accgttacaa agtggtagtg atgaaatttt aaagttaatg ggacgccgct
     1141 atacggttaa agattattta gagttagtta ctaaattacg ggctagaatg cctgatattt
     1201 cactgactac tgatattatt gttggctttc caaatgaaac agagcaacaa tttttagaaa
     1261 cattaaaagt tgttgaaaag gtaaattacg aaaatgctta tacttttatt tattcaccgc
     1321 gcaagggtac tcccgctgcc cttatggaag ataatgtttc gcgtgaagaa aaaggtgagc
     1381 gctttaacaa gttaaaagca ctcgttgata gccaaaccga agctaatgca gcgctatatg
     1441 tcgggaatga gtatcttatt ttagttgatg gttacagtaa aaaagatacg agtgtattaa
     1501 gtggatacac cgaaagtgat cgtgttgttc attttgtagg tactcctgat ttaatcggta
     1561 aaattgttaa agttaaaatt actgagtcac gcgtttattc atttgttgga gaactagtga
     1621 atgaataaag ttgaaatagc cttaaatgca ttaacgactg aattagcgaa tgacaaaaga
     1681 gtcgtagaat ttaaaaaagt aaaagcactg attgaaagtg atgcttactt aaaaaatgca
     1741 gaagcacggc ttaaagagtt acaacgatta atgacacaaa acgcctttaa cgaagaaaag
     1801 cataacgagt ataaaagaga atatttacga ctaaaaaaca attatgaaac gcatccttat
     1861 ctaataaatt ataatagttt gctaagtgaa attgaagatt tactttactc tttaaaaaca
     1921 gtaattgaat aagcaattta ccatactatg gtaaaatgac actatgggaa aagagaagaa
     1981 aaaaatcgtc tacacgccga tgattgagca gtatttagaa attaaacggg aaaacccggg
     2041 tattttaatt atgtatcgac ttggtgattt ttatgagttc ttttttgaag atgctgaagt
     2101 tgtatccaaa gaattacaat tagtgctcac taaaagagca gcaggaaata atcgtaaaat
     2161 cccaatgtgc gggatacccc atcacgctt
//
LOCUS       DAOSEX010000137         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_49930_length_2175_cov_2.606132, whole genome shotgun sequence.
ACCESSION   DAOSEX010000137 DAOSEX010000000
VERSION     DAOSEX010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49930_length_2175_cov_2.606132"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1968
                     /locus_tag="PK612_03095"
     CDS             <1..1968
                     /locus_tag="PK612_03095"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOH95128.1"
                     /translation="SDFYLSVATTRPETMFADVALVINPRDERYTKYLGKNFVNPVNN
                     ELIPLIADDYVDLEFGTGVMKCTPAHDANDFKIGERHNLSRPICLNKDATLNERGLEL
                     AGLDRFDAREKLVAKMKELDLLIKVEKIVHNVGHSERSDAIIEPYLSKQWFLRMDHFA
                     KKSIEGQKGDDPIRFFPKRFNKTYLNWMEIAEDWCVSRQIWWGHRIPAYYHKETGEIV
                     VSVTPPKDLENYRQDEDVLDTWFSSALWPFSTLDWPNTDSELFKRYYPTDVMVTGYDI
                     IFFWVSRMIFMGLELTNEKPFKDVVIHGLIRDEQGRKMSKSLGNGVDPIEVIAKYGVD
                     SLRYFLAGASTPGQDARYSEARIQASVNYLNKIWNAARFLLLNLPEDFKPGTINHKKL
                     HPVDAFILSRYYETVRKVTSLMDKYELGMASNYLYAFVYDDYCSNYIEMVKVILNSDG
                     DHTNTYNVMYHTLHGILMMIYPYSPFISEEIYLALPNHLDSIMLASYPKASRLRFKRE
                     AEEVNTIINLIKEIRNFKSTNNLAPNTKMNLYVADVSFDLTPYLDIIRRLGFINEFKS
                     SDVPIEVTTVSLPKATFGLELDVDRSVLISQLEKEIETLTKEVERSTKLLNNPGFKNK
                     APASKVAEEEEKARHYAESLALAVDKLTKLR"
BASE COUNT          699 a          372 c          439 g          665 t
ORIGIN      
        1 agtgactttt atttaagtgt cgcgacaacg cgaccggaaa caatgtttgc cgacgtcgcc
       61 cttgtcatta acccacgcga tgagcgatac acaaaatact tgggtaaaaa cttcgtaaat
      121 ccagtaaata atgaattaat tccgttaatc gctgatgatt atgttgattt agaatttggt
      181 actggtgtga tgaaatgtac gcccgcgcac gacgcaaatg actttaaaat cggtgaaaga
      241 cataacttaa gtcgcccgat ttgtttaaat aaagacgcaa cacttaacga acgcggtctt
      301 gaactagcgg gtctagatcg cttcgatgca cgcgaaaaac tcgtcgccaa gatgaaagaa
      361 cttgatttac ttattaaagt ggagaaaatc gtgcataatg tcggtcatag tgaacgaagt
      421 gatgccatta ttgaaccgta tttatcaaaa cagtggtttt tacgcatgga tcactttgct
      481 aaaaaaagta tcgaagggca aaaaggtgat gaccccatcc gctttttccc gaaacgcttt
      541 aataaaactt atttaaattg gatggagatt gctgaagatt ggtgcgtatc acgccaaatt
      601 tggtggggtc accgtatccc ggcttattat cataaagaaa ctggtgaaat agtagtaagt
      661 gttacaccac ctaaagactt agaaaattat cggcaagatg aagatgtcct tgatacatgg
      721 tttagcagtg cactttggcc tttttcaacg ctagactggc caaatacgga tagcgaactc
      781 tttaaacgct attacccgac cgatgtaatg gtgacaggct acgatattat tttcttctgg
      841 gtatcacgga tgattttcat ggggttagag ttaactaatg aaaaaccatt taaagacgtc
      901 gttattcacg gtttaattcg tgatgaacag ggccgaaaaa tgtcaaaatc actcggcaac
      961 ggggttgatc caattgaagt gattgcgaag tatggtgttg atagcctccg ttatttctta
     1021 gcaggcgcaa gtaccccagg ccaagatgcg cgctatagtg aagcgcgtat ccaagcaagt
     1081 gtcaattact taaataagat ttggaacgca gctaggttct tacttttaaa cttaccagaa
     1141 gactttaaac caggtacgat taatcataag aaactgcatc ctgttgatgc ttttatctta
     1201 agtcgttatt acgaaacagt gcgtaaagtc acaagtttaa tggataagta tgagcttggt
     1261 atggctagta attatctcta tgcttttgtt tatgatgatt actgctcaaa ttatattgaa
     1321 atggttaaag ttattttaaa tagtgatggt gatcatacga atacatataa tgttatgtat
     1381 cacactttac acggaatctt aatgatgatt tatccttatt caccttttat tagtgaagaa
     1441 atttatttag cgctcccaaa tcatctcgat tctataatgt tagcgagtta tccaaaagct
     1501 agtcgtttac gctttaagcg cgaagcggaa gaagtaaata cgattattaa cttaattaaa
     1561 gaaattcgta actttaagag cactaataac ttagcgccca atactaaaat gaatttatat
     1621 gtcgctgatg ttagctttga tttaacgcct tatcttgata ttattcgccg tttaggtttt
     1681 attaatgaat ttaagtctag tgatgttcct attgaagtaa cgacagttag tcttccaaaa
     1741 gctacttttg gcttagagtt agatgttgat cgcagtgtgc tgattagtca acttgaaaaa
     1801 gaaatcgaaa ctttaacgaa agaagttgaa aggagcacta agcttttaaa taaccctggt
     1861 tttaaaaata aagctcccgc aagcaaagtt gcggaagaag aagaaaaagc tcgccattac
     1921 gctgaaagtc tggcgctcgc ggttgataaa ctaactaaat tgcgataaaa cattaacttt
     1981 ccttttactt aatagtagga ggaaattatt atgactttat taactaatgc cgaaatggca
     2041 aatattaaag gtggtgaacc aatcacttta gcagcggtaa tgacaatttt agtcatcgct
     2101 attgtaacgg tgattgttta taagttattt acttcaaacg ccggttctac gacaataccg
     2161 ggaggtttta aattt
//
LOCUS       DAOSEX010000138         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_49960_length_2174_cov_3.069372, whole genome shotgun sequence.
ACCESSION   DAOSEX010000138 DAOSEX010000000
VERSION     DAOSEX010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49960_length_2174_cov_3.069372"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PK612_03100"
     CDS             <1..776
                     /locus_tag="PK612_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95129.1"
                     /translation="KKKGRIGKITSVVLGAIVVLLIVLQVVGQSTAKNNHGVMRYGNY
                     QSLRVLTDSMEPTYKVDTMVFVKRVDPKTLKGPSSEGALDGDVITFVRNYHGSYDLPG
                     DKRIVTHRIHRIEVQADGRVYFRTLGDNIHAKECPESGCTLEARGDWVRDTDILGKVV
                     GQSMVLGKFSVLLANPIFMLVFAMVPLLIVFGSSLMDLLKQLKSTEGSEATPSGISEQ
                     QAEFERIKEQEKLRLLIELEKEKLRKELEEGIKDETKES"
     gene            757..>2174
                     /locus_tag="PK612_03105"
     CDS             757..>2174
                     /locus_tag="PK612_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOH95130.1"
                     /translation="MKQKKARRHSGKKYLWYSILCVMLLVLADSTYVLMSFGLIPMTG
                     NKTLDIIIGILLVIISIASIVCGIIAFVYYKRNSNQIRFNYATFELVEGINSERAFLA
                     YVAKDIKRNRGKANALVAFAATRFKKIVFTRYGYEKGGEVLGLVYETFNTLKTKYPHS
                     YYGYDYNENFLIYLRDTPYEQVQEYVNDLIEEINKTLFENRNEVKFVADFGVSYCQNE
                     NDEFITAASMLQRALIAADHGRAESDLGGLTLYEPKMYERNKRNVQLGLQIERSLELE
                     QFELHYQPKYDLKLGRFAGAEALVRWRHPELGLLSPANFILFAEQSDLIIKIDHYVLE
                     KVCKQLDEWRGKGQRLLPISVNISKRTLFTGNIINHLIKVTDKYNINPMLLEIEIVES
                     PSLQDILLLLSIVKKFKNLKVKIAIDDFGTGYSSLSYIKKIPFDVIKIDKAFLDDVDI
                     DQKSRLMIKNIIDLAHILDAYVV"
BASE COUNT          728 a          336 c          444 g          666 t
ORIGIN      
        1 caaagaaaaa agggcgtatt ggtaaaatta caagcgttgt tttaggggcg atcgttgttt
       61 tacttattgt tttacaagtc gttggtcaat caaccgctaa aaataatcat ggagtgatgc
      121 gttacggtaa ttatcagtca ttacgcgttt taactgattc aatggaacca acatacaaag
      181 tcgatacaat ggtatttgta aaacgtgttg atccaaaaac actaaaagga ccatcaagcg
      241 aaggagcatt agatggggac gtcattactt ttgtgcgtaa ttatcacgga agttatgatt
      301 tacctggtga taaacgcatt gtcactcacc ggattcatcg tattgaggtg caagcagatg
      361 gccgcgttta cttccggacg cttggtgata atattcacgc taaagagtgt ccagaaagtg
      421 gttgtacatt agaagcgcgt ggtgactggg tgcgtgacac ggatatttta ggcaaagttg
      481 ttggtcaaag tatggtactt ggtaaattta gtgtgttgct cgctaatcca atatttatgc
      541 ttgtgtttgc gatggtacca ctcttaattg tgtttggttc ttcacttatg gacctcctaa
      601 aacaattaaa aagtaccgag gggagtgaag ccacgccgag tggcataagt gaacaacagg
      661 ccgaatttga aagaattaaa gaacaagaga aattacgatt attaattgaa ttagaaaaag
      721 aaaaattacg aaaagaatta gaggagggca taaaagatga aacaaaagaa agctagaaga
      781 catagcggaa aaaagtattt atggtatagc atcttatgtg taatgctttt agtgcttgct
      841 gatagtactt atgttttaat gtccttcggt cttattccta tgacagggaa caaaacactt
      901 gatattataa tcggcatttt attagtaatt ataagtatcg ctagtattgt atgtgggatt
      961 attgcttttg tatattataa gcgaaactca aaccaaattc gctttaatta tgcgacattt
     1021 gaattagtgg aagggattaa ttcagaacga gcgtttttag cctatgtcgc taaagatatt
     1081 aagcgtaata gaggtaaagc aaatgcactt gtcgcttttg cggcaacacg ttttaaaaag
     1141 attgtcttta cgcgatatgg gtatgagaaa ggcggcgaag ttttaggttt agtgtatgaa
     1201 acatttaata cacttaaaac aaaatacccg cattcttatt acggttacga ctataatgaa
     1261 aacttcctta tttatttaag agatacacct tatgaacaag tacaagaata tgtgaatgac
     1321 ttaattgaag aaattaataa aacattattt gaaaatcgta atgaggtaaa atttgtggcg
     1381 gactttggtg tgagttactg tcaaaatgaa aatgacgaat ttattaccgc ggcgtcgatg
     1441 ctccaacggg cactcattgc agctgatcac ggtcgtgccg aaagtgattt aggtggttta
     1501 acactttatg aaccaaagat gtatgaacgt aataaacgaa atgtccaact tggtttacaa
     1561 attgaacgca gtttggaatt agaacaattt gaactgcact atcaaccaaa atatgactta
     1621 aaattaggcc gctttgcggg cgctgaagca cttgttagat ggcgccatcc agagttagga
     1681 ctactctcac ctgcaaactt tatcttattt gccgaacaaa gtgacttaat tattaaaatt
     1741 gaccattatg tgctggaaaa agtgtgtaaa caacttgatg aatggcgggg taaaggacaa
     1801 cgcttattac caatcagtgt caacatttca aaacgaacac tctttactgg gaatattatt
     1861 aatcacctaa ttaaagtaac agataaatat aatattaacc cgatgttgct tgaaattgaa
     1921 atcgttgaat caccatcact gcaagacata ttacttttac tctcaattgt gaagaaattt
     1981 aaaaacttga aagttaaaat tgccattgac gactttggga ctggttactc ttcgcttagt
     2041 tatattaaaa agattccctt tgatgttatt aaaattgata aggctttcct tgatgacgtt
     2101 gatattgacc aaaaatcacg attaatgatt aaaaacatta ttgatttagc gcacattctc
     2161 gacgcctatg tcgt
//
LOCUS       DAOSEX010000139         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_50275_length_2165_cov_2.448341, whole genome shotgun sequence.
ACCESSION   DAOSEX010000139 DAOSEX010000000
VERSION     DAOSEX010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50275_length_2165_cov_2.448341"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK612_03110"
     CDS             <1..415
                     /locus_tag="PK612_03110"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOH95131.1"
                     /translation="VEKIQQMLLTKTGKHIEVLIYGDGAFKDPQGHIWELADPVVSPF
                     FTPGLSGTPSEVKLKYLADNDFKNLTGAELTKAIAASIKAKPKDLVGTKATLGTTPRQ
                     ITDLVGSLCDLVSGSGDKGTPVVLIQGYFDNYTDN"
     gene            complement(416..955)
                     /gene="hpt"
                     /locus_tag="PK612_03115"
     CDS             complement(416..955)
                     /gene="hpt"
                     /locus_tag="PK612_03115"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOH95132.1"
                     /translation="MMMKDLEKVLVTEAEITEIINRVSARIEKDYKGKDLVMIGNLKG
                     GAPFMMDLIKKINLPLIIDFIRVESYFGGTTQGELVFRKDIEVDITNKHVLIVDDIID
                     SCRTLKMVIDLFKKRNASSIEVCTLLDKPSGRVIDYTPKYIGTTIGNEFVVGYGLDYD
                     EKYRNLPVIGVLKEEVYKK"
     gene            complement(955..>2165)
                     /locus_tag="PK612_03120"
     CDS             complement(955..>2165)
                     /locus_tag="PK612_03120"
                     /inference="COORDINATES: protein motif:HMM:NF020477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOH95133.1"
                     /translation="EVERLIDEFRTKDIAVCGVVFSFFSESIVTNAFIKKLARNNIKT
                     YKHYVIEDYPHNLEKIFSEDGLGRNEYVETTRPLVVVTAPGSGSGKLATCLSQLYHDN
                     KNGIRAGYAKYETFPIWNLPLNHPLNLAYEAATVDLGDFNVIDPYYLDKFNVKATNYN
                     RDVDAFPVLKAIFEKMYGETPYESPTMMGVNMVGFALGDEEAITAACKEEVIRRFYSA
                     QKNLFLDKYTASSLDKIRMIMNKLGIVETDRKCVLPALKKADETHVPCMALVLNDGKI
                     ITAKTSKLLSPAAALFLNSVKYLANINDEIPLISPNLIEPIEALKKEISAINDSVLNL
                     NDTLISLAIQAPMNPLSDLALKQIEKLKGAQAHSSVLLDKRDRQVLKQLGINVTEESV
                     PASNKLYWRS"
BASE COUNT          680 a          432 c          359 g          694 t
ORIGIN      
        1 tgtcgaaaaa attcaacaaa tgttactaac aaaaacaggc aaacatattg aagttttaat
       61 ttatggcgat ggtgctttta aagatccaca aggacatatt tgggaactag cagatcctgt
      121 agtgtcacca tttttcactc ctggattaag cggcacacct agcgaagtaa aactaaaata
      181 tctcgctgat aacgatttta aaaatttaac gggcgctgaa cttactaaag caattgccgc
      241 ttcaattaaa gctaaaccaa aagaccttgt aggcactaag gcgacgctag gtactacccc
      301 acgtcaaata actgaccttg ttggatcact ttgtgattta gtaagtggtt ctggtgataa
      361 aggcacacca gttgtgttaa ttcaaggtta ttttgataat tacacagata attaattatt
      421 ttttataaac ttcttctttc aatacaccga taactggcaa gttacgatat ttttcatcat
      481 aatctaaacc gtaaccaaca acaaattcat taccgatagt tgtcccaata tactttggcg
      541 tataatcaat tacgcgacca cttggtttat caagtagtgt acacacttca atcgaactag
      601 cgttacgctt tttaaagagg tcaattacca tttttaatgt gcggcagcta tcaataatat
      661 cgtcaacaat taagacatgc ttgttagtga tatcaacttc aatatcctta cggaagacta
      721 gttcgccttg tgtcgtgcca ccaaaataag attcaacacg aataaagtcg atgataagtg
      781 gcaaattaat tttcttaatt aaatccatca taaacggggc gccgcccttt aaattgccaa
      841 tcatcactaa atctttaccc ttataatcct tttcgatccg tgctgaaaca cgattaataa
      901 tttccgtaat ttctgcttca gtcactaata ctttttctaa atccttcatc ataatcatga
      961 cctccaatat aatttatttg atgctgggac actctcttca gtaacattaa ttcctagttg
     1021 ttttaaaact tgacgatcgc gtttatcaag gagcacagaa gagtgcgctt gtgctccttt
     1081 taatttttca atttgcttaa gcgcaagatc cgataacgga ttcatcggcg cttggattgc
     1141 tagagaaatc aatgtatcgt ttaaattaag cactgaatcg ttaatcgcac ttatttcttt
     1201 tttcagcgct tcaattggtt cgataagatt tggggaaata agcggaattt catcattaat
     1261 attcgctaaa tatttaactg aatttaagaa taatgcagcg gcagggctta aaagttttga
     1321 tgtttttgct gtaataattt tgccgtcatt aagcactaaa gccatacacg ggacatgtgt
     1381 ttcatcggcc tttttaaggg caggcaatac acatttacga tcagtttcaa caatgcctaa
     1441 tttattcata atcatccgga ttttatcaag actactagcg gtatatttat caaggaagag
     1501 gtttttttgc gctgaataaa aacggcgaat aacttcctct ttacacgcgg cggtaatcgc
     1561 ttcttcatcc ccaagcgcaa agccaaccat attgacgccc atcattgtcg gtgattcata
     1621 gggcgtttca ccatacatct tctcaaaaat agcttttaac acagggaaag cgtcaacatc
     1681 acgattgtaa tttgtcgctt taacattaaa tttatctaag taataaggat caataacatt
     1741 aaagtcacct aaatcaacag tcgctgcttc ataggctaag ttaagcgggt gattaagcgg
     1801 taaattccag attgggaatg tttcatattt tgcatatccc gctcttatac cgtttttatt
     1861 atcgtgatag agttgcgata aacatgtcgc gagtttccca cttcctgaac caggtgcagt
     1921 aacaaccaca agcggacgcg ttgtttcgac atattcatta cgacctaacc cgtcttcact
     1981 aaatatcttt tctaagttat gcggatagtc ttcaatcacg taatgtttat atgtcttaat
     2041 attatttctc gctagttttt tgataaaagc attcgtgaca attgattcac taaaaaagga
     2101 aaagacaaca ccacaaacgg cgatatcttt agtgcgaaat tcatcgatta accgttcaac
     2161 ttcac
//
LOCUS       DAOSEX010000140         2145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_50990_length_2145_cov_2.348804, whole genome shotgun sequence.
ACCESSION   DAOSEX010000140 DAOSEX010000000
VERSION     DAOSEX010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50990_length_2145_cov_2.348804"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PK612_03125"
     CDS             complement(<1..712)
                     /locus_tag="PK612_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOH95134.1"
                     /translation="MKAFIFNSGVGSRLGDLTKDKPKALVELKSGETILGRQLRILRS
                     QGIKEIVISTGYKEEMIKEYCLKEFPELHFTFVFNPDYATTNSIYSMYLAENKFQDDF
                     LIMHGDLVFDEGIVEKLLEEKHRDLVCINTEIPKPPKDFKGRIKKEKLQKIAVDIFAK
                     DDYALQPLYKLSFATISQWMLRIKEMIGKGIVDVYAENALNEALLDLDVRCVDYKYNF
                     AEEIDTLEDYERVSNDVRL"
     gene            complement(714..1832)
                     /gene="aepY"
                     /locus_tag="PK612_03130"
     CDS             complement(714..1832)
                     /gene="aepY"
                     /locus_tag="PK612_03130"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HOH95135.1"
                     /translation="MIDTKSFYDYLIEKGIDTFFGVPDSLLKDICAYITQNTTPEKHI
                     ITANEGNAVALAAGHYLVTGKPAMVYLQNSGLGNIVNPLISLADEDVYNIPVLFMVGW
                     RGEPGTKDEPQHKKQGKITLSTLEVLGVKTFILEDEFKPIIDEAVSYMKKTLKPAAIV
                     VKKGTFSKVILELPPNTFSMTREEALEVMMKHIDNKSVIVSTTGKTSREVFEIREKSG
                     DTHERDFLTVGSMGHTASIALGIALSSKKRVFCIDGDGSMLMHLGGLGVAAINAQKNF
                     KYIVINNGAHESVGGQPTIALDINTKKVLEGLGFSKVYEVKTLEEIEREFPKFKRKNK
                     AALVVYVNQGSRDDLGRPTTTPVENKDLLMKFLRRSRK"
     gene            complement(1835..>2145)
                     /locus_tag="PK612_03135"
     CDS             complement(1835..>2145)
                     /locus_tag="PK612_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002671371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HOH95136.1"
                     /translation="DGVMIHSKNKTGEDIFEFIRRFREYSKDIPIVLVPTSYNQFTEK
                     ELAEKGANIIIHANHLLRAAYLAMVKAAETILENGRSKEADEFCLPIKKVLRLIPGGE
                     "
BASE COUNT          656 a          423 c          290 g          776 t
ORIGIN      
        1 aaagcctaac atcattacta actctttcgt aatcttctaa agtatcaatt tcttccgcga
       61 aattatactt ataatcaacg catcgcacat ctaaatcaag tagtgcttca tttaaagcat
      121 tttctgcata aacatcaaca atacccttac caatcatttc cttaatacga agcatccatt
      181 gactaattgt ggcaaaactt agtttatata atggttgcaa tgcataatca tcttttgcga
      241 agatgtctac agcaattttt tgaagttttt ctttcttaat tctgccctta aagtcttttg
      301 gtggttttgg gatttcagta tttatacaga ctaaatcgcg atgtttttct tccaatagtt
      361 tttccactat tccttcatca aacactaaat caccatgcat aatcaaaaaa tcatcttgga
      421 atttattttc cgctaaatac attgaataga tgctattagt agtagcataa tcagggttaa
      481 atacaaaagt aaaatgtaac tctggaaact cttttaaaca atattcttta atcatttctt
      541 ctttgtaacc agttgaaata actatctctt taataccttg acttcttaaa atacgaagtt
      601 gtcgtcctaa aatggtttca ccacttttta attccactag tgcttttggt ttatcttttg
      661 ttaaatcacc caaacgtgat ccaacaccac tattaaatat aaatgctttc atattattta
      721 cgacttctcc ttaagaattt cattaataag tctttgtttt caactggggt agttgtgggc
      781 cgccctaaat catcacgact tccttgatta acatacacta ctaatgcagc tttatttttc
      841 cgtttaaact ttggaaactc tctttcaatt tcttctaaag ttttaacttc ataaacttta
      901 ctaaaaccta gtccttctaa tactttctta gtattaatat ctagcgctat agtcggttgc
      961 ccccctactg attcatgtgc tccattgtta ataacaatat atttaaaatt cttttgcgcg
     1021 ttgattgccg ctacgcctaa tccgcctaaa tgcatcagca tagagccatc cccatctata
     1081 caaaatacac gctttttgct tgataaagca ataccaagtg cgattgatgc agtatgtccc
     1141 attgaaccaa ctgttaagaa gtctcgttca tgagtatcac cacttttttc tctaatttca
     1201 aatacttcac gacttgtttt accagtagtg ctaacaataa cactcttgtt atcaatatgt
     1261 ttcatcatta cttcaagtgc ttcttcacga gtcatagaaa aagtattagg aggtaattct
     1321 aatataactt tactaaaagt tcctttttta acaacgatgg ccgctggttt aagtgttttc
     1381 ttcatataac taacagcttc atcaataatc ggtttaaact catcttcaag aataaatgtt
     1441 ttaactccta atacttcaag agtgctaagt gttatctttc cttgtttttt atgttgcggt
     1501 tcatcttttg tcccgggttc tccgcgccaa ccaaccataa aaagaacagg aatattataa
     1561 acatcttcgt ccgctaaaga aatgagagga ttaacaatat tacctaaacc actattttgt
     1621 agataaacca ttgctggttt accagttact aaataatgac cagcggctaa cgctactgca
     1681 ttgccttcat tagcggtaat aatatgtttt tctggggttg tattttgggt aatataggca
     1741 caaatatctt ttaataaaga atcgggtact ccaaagaaag tgtcaatccc tttttcaatt
     1801 aaataatcat aaaaactttt ggtgtcaatc atctttattc acctcctgga attaagcgca
     1861 atactttttt aatcggtaga cagaattcat cagcttcttt gctacgacca ttttccaaaa
     1921 tggtttctgc tgctttgacc atggcaagat aggcagctct taataaatgg ttagcgtgaa
     1981 taataatgtt tgcacctttt tccgctaatt ctttttctgt aaattggtta taagaagttg
     2041 gaacaaggac aatgggaata tctttactat attctctaaa tctacgaata aattcaaaga
     2101 tatcttcacc ggttttattt ttagagtgaa tcatgacgcc atccg
//
LOCUS       DAOSEX010000141         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_51211_length_2139_cov_2.889635, whole genome shotgun sequence.
ACCESSION   DAOSEX010000141 DAOSEX010000000
VERSION     DAOSEX010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51211_length_2139_cov_2.889635"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            441..1790
                     /locus_tag="PK612_03140"
     CDS             441..1790
                     /locus_tag="PK612_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95137.1"
                     /translation="MKNKKAIALILINVLALGVMGVLHSLRKNGLLGGVDANPLSPAY
                     TRTLTALDGPNGTMPMRGTIVAKDDTYYYSSASSQNDDSFLVLSASDAKNPGVFMNTT
                     VINGIGEVRVTFSGSGNLYAIVSETLFERFTPSASDALTSGVKKEFATVGGYLLILND
                     SLSPITIESLEIYFHCAHDIDSKFYFEQGVNLYTGARSLGSNIVIGHDQLSFKTNPTA
                     TTNNYAGETIVPPAERPNAWYRWNGVSMRNYGGTLETPNYTTTRFGEFYSSSFKISTT
                     AFVDPSVFYDTDEWFCVAPWVSLSASGKPRTERHYLQSYIGNDNYDPLTTVNELYKPY
                     FSGRFYTNYGYTGSAWEFQDPDTVTVVDDATMTLREAYEAINLPFFNVTYEISGNTYK
                     VFINGFYVYTDTILETYTDETFALETFELQAVNYGNEDGSPKAGYNVTYSNPVIKTI"
BASE COUNT          682 a          373 c          421 g          663 t
ORIGIN      
        1 aaccgataga ggatgcaagt cccgcctgag ttcgaatctc agtgcttccg ccatgtaaat
       61 cttactttgg taagtaaata ctgccaaaac gcgcataaat tagtccttaa tggacttttt
      121 ttgttggttt cgggcggacc gaagaaaaat tttgtttcgt tcgaacggcc atgctctttt
      181 tggggtcgtc ccttacgcag tttccatatt aagatagcga actctctttt ctaaatctcc
      241 atactaagat agcgaactct caaacaaaaa tataaaaagt cgcttcttgg attgaatgta
      301 gtgcgcccca gaaattggac caattgttat cactaataaa tattcaaatt tttttgtata
      361 gaaagcgcct ttattttaaa aaacgcactt tgacatttat tgtaagttat tatatactta
      421 agaaaaaagg aataaatatt atgaaaaaca agaaagcaat agccttaatt ctcattaacg
      481 ttttagccct tggtgtaatg ggggtcttac attcattacg caaaaatgga ttattaggtg
      541 gagtcgacgc taatccgctt tcaccggcgt atactcgaac tttaaccgcg cttgatggac
      601 caaacggaac tatgccaatg cgcgggacaa ttgtggctaa agatgatact tattattatt
      661 caagtgcaag ttcccaaaat gacgatagtt tccttgtttt atccgcaagt gatgctaaaa
      721 accctggtgt atttatgaac acaactgtca ttaacggaat tggggaagta agagtaacat
      781 tttcaggcag cggaaatctt tatgcaatag taagtgaaac attatttgag cgttttaccc
      841 ctagtgcaag tgatgctcta acaagtggcg ttaaaaaaga atttgcgaca gttgggggtt
      901 acttactaat tttaaatgat tcactaagcc caattacaat tgaaagttta gaaatatatt
      961 tccattgtgc gcatgacatt gatagtaaat tttactttga acaaggcgtt aatctctaca
     1021 ctggtgcgcg gtcattagga agtaatatag ttatcggtca tgaccaactc tcctttaaaa
     1081 ctaatccgac cgccacgaca aataattatg ccggggaaac aatagtccct ccggccgagc
     1141 gccctaatgc atggtatcga tggaatggtg taagtatgcg taattatggg ggcacattag
     1201 aaacaccaaa ttatacgacc actaggtttg gagagtttta tagttcttca tttaagataa
     1261 gtacgacagc ttttgttgat ccaagtgtgt tttatgacac tgatgaatgg ttttgtgtgg
     1321 cgccatgggt aagtttaagt gcatcgggaa aaccgcgaac agaacgccat tacttgcagt
     1381 catatatcgg taacgataat tatgacccac taacgacggt taatgagctt tataagccgt
     1441 atttttccgg tcgtttctac actaattatg gttataccgg aagtgcctgg gagttccaag
     1501 acccagatac tgttactgtt gttgatgatg cgacaatgac attaagagaa gcatatgaag
     1561 caattaattt accgtttttc aatgttactt atgaaataag tggcaataca tataaagtgt
     1621 ttattaatgg attttatgta tatactgaca cgattcttga aacttacacg gatgaaactt
     1681 ttgcgcttga gacttttgaa ttacaagctg tcaattatgg gaatgaagat ggttcaccaa
     1741 aagctggtta caatgttact tattctaacc ctgtcattaa aacaatttaa aatattttaa
     1801 aaatacatgg caagcaggct taataagtag gcctgctttt ttaaaatata taatgtgtta
     1861 aatatattca catttctaaa taaaagtaac gaccctcttt aatttaaaac tgttacaata
     1921 ataaagtgcc tttaaaaatt aagtgaacct aaacattgcg atgttttaat tgaaagagaa
     1981 acaaatttat gatttattta acggattttt tcggtattgg cggagctccg cggcaaacga
     2041 ctaatcatgg cggccgaaag ccaggaaggg gcgcgtctca acgattcgat agtcaagcag
     2101 ctatgttcga ccgatgctat tttcgctgag aagaagtat
//
LOCUS       DAOSEX010000142         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_51693_length_2125_cov_3.300483, whole genome shotgun sequence.
ACCESSION   DAOSEX010000142 DAOSEX010000000
VERSION     DAOSEX010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51693_length_2125_cov_3.300483"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PK612_03145"
     CDS             <1..586
                     /locus_tag="PK612_03145"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOH95138.1"
                     /translation="IFEKNKKDVFKDKYATAIELTEAGKEQEVLVVPPSIRGFPVRYI
                     GKPKRFFQPLDINALKLTDKQIKIYLPYSLRGQMWLASVRTVEAILALANLDDFDKVL
                     TINSFPEIKLHYLNGKNKLNTFYMYNFESSLNEGYYFMDYINGSNPYVIPSDPVRKGY
                     TFAGWYYQKECATLWNNEVPKSESESLTLFAKWI"
     gene            673..1278
                     /locus_tag="PK612_03150"
     CDS             673..1278
                     /locus_tag="PK612_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95139.1"
                     /translation="MKNKKKLFWLILPFTLIMFALLFLRYPIMPTNGTYKVFHSPKKH
                     VENVTTLIHQKKDTIDVEWEDVEVTPLYNYKDNFNYLLVDLYPQGFTILKVIRTSPFS
                     VKASQYAMPSVYGRHYYYSDEDTSPQYTSIYAERDVKNEKLYLLQVKNSHGHGHIPAI
                     KGDNCYINLLTLEEIPLNYYEIDEPYAMYEWWFAYMVAFRF"
     gene            complement(1302..1628)
                     /locus_tag="PK612_03155"
     CDS             complement(1302..1628)
                     /locus_tag="PK612_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOH95140.1"
                     /translation="MLKDTNINVRVNSDIKNEAETILNKLGLNLSSAIDLYLAQIIKT
                     RGIPFEVKLPNEENKEKVLQIGQIINSLGGAPTTPELNKIIELYAQNDISYDVAIYAI
                     KEILNK"
BASE COUNT          728 a          310 c          353 g          734 t
ORIGIN      
        1 tatctttgaa aagaataaaa aggatgtatt taaagataaa tatgcaacgg caattgaatt
       61 aaccgaggcc ggaaaagaac aagaagtttt ggttgtgcct ccaagcatta gaggttttcc
      121 agttcggtat ataggaaaac caaaacgatt ttttcaaccc ttagatatta atgcactgaa
      181 gttaacagat aagcaaataa agatttatct tccatattcc ttgcgtgggc aaatgtggtt
      241 agcgtcagtg aggactgtcg aagctatcct tgctttggct aatctagatg actttgataa
      301 ggtattaact attaattcat ttcctgaaat taaattacat tatctaaacg ggaaaaataa
      361 acttaatact ttctacatgt ataattttga atcttcccta aatgaaggct attactttat
      421 ggattatatt aatggtagta atccttatgt cataccttct gaccctgttc gtaaaggata
      481 cacttttgcg ggatggtatt atcagaaaga atgcgctact ttgtggaata acgaggtgcc
      541 taagtcagaa agtgaatcgt taacgctttt tgctaagtgg atttaactaa actaagagat
      601 gctgatttag atgcagtggt aaacttgata ttcaagaaaa aaatatacaa ttacaacagg
      661 gaggaaacaa taatgaaaaa taaaaagaaa ttgttttggt taatattacc gtttacttta
      721 attatgtttg ctcttttgtt tctgcgctat cctattatgc cgacaaacgg gacatataaa
      781 gtttttcatt caccaaagaa acatgttgaa aatgtaacaa cgcttatcca tcaaaagaag
      841 gacacaatcg atgttgagtg ggaagatgtt gaagtaacac cactttacaa ctacaaagat
      901 aacttcaatt atttattagt ggatctgtac ccccaaggat ttactatttt aaaagtaatc
      961 cgcactagtc cgtttagtgt taaagctagt caatatgcaa tgcctagtgt ttatggtcgt
     1021 cattattact atagtgatga agatacttca ccacaataca ctagtattta tgctgaacgt
     1081 gatgttaaaa atgaaaaatt atatttgtta caagttaaaa attcgcatgg acatggtcat
     1141 attcctgcaa ttaaaggtga taattgttat atcaatctat taactttaga agaaatacca
     1201 cttaattatt atgaaataga tgaaccttat gctatgtatg aatggtggtt tgcatatatg
     1261 gttgcttttc gcttttaata caataatagt gtttcacaat attatttatt taatatttct
     1321 ttaatcgcat atatcgctac atcgtagctt atatcatttt gcgcatagag ttctataatc
     1381 ttattaagtt ctggggttgt aggagcgcca cctaatgaat taataatttg tccaatttgt
     1441 aaaacttttt ccttattttc ttcgttgggc aatttaactt caaaggggat gccgcgcgtt
     1501 ttaataattt gcgctaaata taaatcaata gcgctagaaa gattaagccc tagtttattt
     1561 aaaattgttt cggcttcgtt tttaatatca ctattcactc ttacatttat atttgtgtct
     1621 tttaacatcg tttctatcaa atttatttta ctaatatgta ttaacattgt caatacagag
     1681 tctgatttta agggttaaaa ttaatataaa aaccccataa aatgcgactt taatggttta
     1741 aattacaaaa agtgaaaatt atatatcata ttatttgaat cacactttaa gttgtggttt
     1801 aatatgtcaa gtgtccgccc tggagcggct ccatcataac tagaaaaaaa gttgtaaaaa
     1861 atgagcatat tgttgacaca actttaaaat gatggtatta tatgaaagcg ctatattccc
     1921 cgagagtata ttacttcgta ataaagggga tgcgaagcgc tcttgatctt tgaaaactga
     1981 acagatgtac aaataaacaa cgtcaatttt ttattgaatc aatacttttg aactttaata
     2041 ttatgaacta ggataaacac attaaattta attaataact atatttattg agagtttgat
     2101 cctggctcag gatgaacgct ggcgg
//
LOCUS       DAOSEX010000143         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_51849_length_2121_cov_2.761859, whole genome shotgun sequence.
ACCESSION   DAOSEX010000143 DAOSEX010000000
VERSION     DAOSEX010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51849_length_2121_cov_2.761859"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="PK612_03160"
     CDS             complement(<1..767)
                     /locus_tag="PK612_03160"
                     /inference="COORDINATES: protein motif:HMM:NF028185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 36 N-terminal
                     domain-containing protein"
                     /protein_id="HOH95141.1"
                     /translation="MEYKYIHLKTNDTSYVLAINKYGHLENLYYGAKLALNEDLSILV
                     NKQTNMQGTTTVYSGEPHDTYSLDFVNLELGTIGKGDYRRPSILLEHELGFASDFVVK
                     KHGFAGREVLLVNDLPIPHGYDEYFYVILEDNISKVKVQLDYLIFKRANVIARRLVVL
                     SGKGKLTKAASLNLDLNNDHFDVFSTYGTWANEVHASRNKLVPGLFEVATSVGPSSNR
                     HNPFILLLNEKTDLHKGIGYGVNLIYSGPFKASVELT"
     gene            843..>2121
                     /locus_tag="PK612_03165"
     CDS             843..>2121
                     /locus_tag="PK612_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH95142.1"
                     /translation="MKHALNYNWSFIPKFNDEYIKSIPQGVISVDLPHNMVDFPFNYF
                     DEKDFDSVGTYFREIVFSKVLESDKFVLRFGGVNNSFTLYVNSQLVGTFTRPYMEENI
                     DITKYVVEGNNLIVVCVNGSEKVNAPPFGNTVDYFPFSGIYREVTLFVYNALDIIDVK
                     VTANHLGQIEVNPDIRNINKVDYSVNYEVYEGTKLIASSIEHNFTVENPKLWSVNNPN
                     LYNLVTTITANKDVKQYVSVFGFRSAVFLSDGFFLNGEYTPLVGLNRHETYPYVGAAA
                     TSFLQFYDVVKLKNLGVNYVRCSHYPPSRAFLDACDFLGLMVINEVPGWQYIGDEAWR
                     KIHLQNIETMINRDFNRPSIIAWSIRINESADHPFYEECHKLAKALDPVRATTGTRNF
                     TNSELFEDIYSYNDFSHDGTNKGLLAKRKVTKSK"
BASE COUNT          681 a          378 c          362 g          700 t
ORIGIN      
        1 aaagttaatt ctaccgaagc tttaaatggt ccgctataaa ttaaattaac accataacct
       61 atccctttat gtaaatcagt tttctcgttc aaaagtaaaa taaacggatt atgacgattg
      121 ctacttgggc caacacttgt cgccacttca aagagaccag gcactaattt attacgcgaa
      181 gcatggactt catttgccca tgtgccataa gttgaaaaca catcaaaatg atcattattt
      241 aaatccaagt ttaaacttgc tgcttttgtt aatttacctt ttccgctaag gacaacaaga
      301 cgccgagcga tcacattagc gcgcttaaaa attaagtaat ctaattgtac ttttacttta
      361 ctaatgttgt cttcaaggat gacataaaag tattcatcat agccatgcgg aattggtaaa
      421 tcatttacta aaagtacttc tcttccggca aaaccgtgct tttttacgac aaaatcactc
      481 gcaaatccta gttcatgttc aagtaaaata cttggtcgcc ggtaatcacc tttgccaatc
      541 gtccctaatt ctaaattaac aaaatctaag gaataagtgt catgcggttc tccgctataa
      601 acagttgtcg tcccttgcat attagtttgt ttattaacta aaatgcttaa atcttcattt
      661 agcgctaact ttgcgccata gtaaaggttt tctaaatggc catatttatt aatggcaagc
      721 acatatgatg tgtcatttgt tttaaggtgt atatatttgt attccataga ttaattttaa
      781 catacatacg ctatttttaa tatttactaa gtaattatca tttaatgtta aaatatgtat
      841 gtatgaaaca cgcactgaac tataattggt cattcatccc aaaatttaat gatgaatata
      901 ttaagtctat cccgcaaggg gtaataagtg ttgacctccc gcataatatg gtcgactttc
      961 cttttaatta ctttgatgaa aaggattttg atagtgttgg tacttacttc cgtgaaatcg
     1021 tttttagcaa ggtacttgaa agtgataaat ttgtcttacg cttcggtggc gtaaataatt
     1081 cttttacatt atatgtaaat tcgcaattgg tcggtacatt tactagacca tatatggaag
     1141 aaaatattga tattacgaaa tatgttgtcg agggcaataa tttaattgtt gtatgtgtta
     1201 acggcagtga aaaagttaac gctccaccat ttggtaatac cgttgactac ttccccttta
     1261 gcggaattta ccgtgaagta acactttttg tatataacgc attagatatt attgatgtaa
     1321 aagttaccgc taatcattta gggcaaattg aagtaaaccc cgatattaga aacattaata
     1381 aggttgatta ctctgtaaat tatgaagtat acgaaggcac aaagcttatc gctagtagta
     1441 tcgagcataa tttcacagtc gaaaacccca aactttggag tgttaataat cctaatttat
     1501 acaatttagt tactactatt accgctaata aagatgttaa acaatatgtt agtgtctttg
     1561 gttttcgcag tgccgtattt cttagtgacg gcttctttct taacggtgaa tacacgcctt
     1621 tagttggact taatcgtcat gaaacttacc catatgttgg cgcagcagcc acgagttttc
     1681 tacaatttta tgatgttgta aaacttaaaa accttggtgt taattatgtg cgttgctcgc
     1741 actacccacc aagtagagct tttcttgatg cctgtgattt tttaggactt atggtcataa
     1801 atgaagttcc aggatggcag tatatagggg acgaagcgtg gcgaaagatt catttacaaa
     1861 acattgaaac gatgattaat cgtgacttta atcgtccaag tattatcgcg tggtccatta
     1921 ggattaatga aagcgctgac cacccattct acgaagagtg tcataaacta gcaaaagcac
     1981 ttgaccccgt tcgcgctaca acaggaacac gtaactttac taatagcgaa ctttttgaag
     2041 acatctactc ctataatgac ttcagtcatg acggcacgaa taagggatta ttagctaagc
     2101 gtaaagtaac aaaaagtaaa a
//
LOCUS       DAOSEX010000144         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_52016_length_2116_cov_3.814653, whole genome shotgun sequence.
ACCESSION   DAOSEX010000144 DAOSEX010000000
VERSION     DAOSEX010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52016_length_2116_cov_3.814653"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..389)
                     /locus_tag="PK612_03170"
     CDS             complement(186..389)
                     /locus_tag="PK612_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOH95143.1"
                     /translation="MSPINRHKLIIEIISENHSIGADYLAKKCNVSIITIKRDLKKLS
                     DAGQIKYVGSAKKGRWIIIEKKQ"
     gene            complement(480..1895)
                     /locus_tag="PK612_03175"
                     /pseudo
     CDS             complement(480..1895)
                     /locus_tag="PK612_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          618 a          367 c          279 g          852 t
ORIGIN      
        1 aattagaaaa aatatgtgat gtatttaata ttgttttatt ttctatggaa aatgtttttt
       61 tatctatatt ccaaatatta atgatttttt ttaattttca caacttggga ttctgttaaa
      121 taatcgtcct gtgagaataa atataatcca tttaataatt taggcatatt attaatatat
      181 atttcttatt gcttcttttc aataatgatc caccgaccct ttttagcgct accaacatat
      241 tttatttgcc cagcatcact taattttttt aaatctcttt taattgtgat tatcgagaca
      301 ttacattttt ttgctaaata gtctgcacct atgctatgat tttcactgat tatttctatt
      361 attaatttat gccgattgat tggactcatt cttattattg aagtttccat agtttgggta
      421 tcattttgag tatcattttg agtatcattt tgggtatcat ttcgcttgtc tagtaaaatc
      481 catctcccat ccttagtact accttcatat ttaatttttc cagcttctat taaccttgtt
      541 aaatctcttt ttgttgttct tattgatgtt atgagcatat ttgacaattc ttttgctgtt
      601 atagttggat tatcttttaa aatatttaat atctcttgtc ttctattttt tatattttgc
      661 ttttgagtca gtttttcggt gccactttcg gtgccacttt cggtgccact ttgggtgcca
      721 ttttcaccga catttatttc accataattt aggttcttta agattgtgaa aaaggctgat
      781 tcagtggatt taaattgtgg cataaacttt gcattgtaat tatgttcagc ttcataagct
      841 tccacaattt tctttaaccc agaacctctt cgttccatta aattaaaacg accaaataag
      901 tcagcgatga ctggatttct tctaattgat ggaatactaa aaatatctag ttcttgaata
      961 aaacttccgt catacatgcc acctggtgag actatctcga ttctatcatc ataaacatca
     1021 acatgaattt ctcccccaat aatagtgtaa tcacggtgaa caagcgcgtt cactaatgct
     1081 tccttaattg cgagttctgg ataatcaaaa tattcctcac ggtaaagagc gcccttgcgc
     1141 cacatctttt tattattacg ttttataaag tcaagtgccg cttttaataa atacaataaa
     1201 ttaccagcgt attcttggtc gtctaaggct tccattaacc cgtgcgtttt agttagtccg
     1261 ttccatcttg tgcaaaagag acgcgactga taaacgacat ggccatcggc aaaaagcgca
     1321 ccagcgttcg ttaagattcc tttatcattt actaacttaa atgaagaata atctttggtt
     1381 tctaattcta agcgtgtgac tattttatat tcttgttcta acattttaaa agttacatca
     1441 tcaatatgtt tattagatag taataaatcg tatgttagtg tttgcccttt aagtgttaga
     1501 ttaagtaaat cagtacttgt tgctcttaca ctttgattac caattcgttt atacgctgtt
     1561 cgagcgctac cttcaataaa gtagtaaggt gtattatgac cagcaaaaac caataaaata
     1621 attattgtcg caccttttgt ctccgtaatt tccatattaa attcaggaat tggatcaatg
     1681 cgtgttttaa tagtttcact aataaattcg gaatcacttt tgtaattttc tagaccttta
     1741 acttcacctg agtcactaac accaaaaaag atttttcctc ctttgctgtt agcaaaagca
     1801 acaacacttt ttaaccacgt tttaattttc gtgcgttcta ctttttcttt tttatcataa
     1861 ttatttgttt cacctacata agcttttagt aacattaaaa gaatcacccc tatttgtaaa
     1921 tagagaagat atctactttt taaaacaaaa aagtttttct aatcaattag caaaactttg
     1981 gcttttcaac ttttttccga tttgaataga aaaattatta tatagcgtta tcttattttt
     2041 tattacctat aattatagta attttaattg cttcaaatta catttttata acatagaatg
     2101 tatttttgcc tgcgcc
//
LOCUS       DAOSEX010000145         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_52029_length_2116_cov_2.652596, whole genome shotgun sequence.
ACCESSION   DAOSEX010000145 DAOSEX010000000
VERSION     DAOSEX010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52029_length_2116_cov_2.652596"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2116
                     /locus_tag="PK612_03180"
     CDS             <1..>2116
                     /locus_tag="PK612_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH95144.1"
                     /translation="ILLSALFAFSSVRASSNIVRTEAWSGTQTSTEGTYYSTVGSETG
                     TALLNKLKTITGNPTPQTSYDWYRYEAADEAENESNKVLLIYSRQKVLKTAHVSGSTG
                     WNREHTYPDSKISGAAESDNHHIFADDNRTNSTRGNMPFGVVAQTSTNQVKDGYGNLT
                     DCYKDGTYFYPNEPARGEVARATMYVNMRYGYSVTGNFQSIALMLEWHLQNPVSNREI
                     YRNNTVHTMQKNRNPFIDHPEYACRIWGNTNSETQAICGAAQQVDVTSVSVAPSSGSI
                     NLLDVNETLQLSANVLPANATNKSVNWSTSNSSIATVDANGLVTGKAVGNVTITATSV
                     SNPSVKGTASIAVSNTSIPVTGVTISDKSFTLVKDATKTLTATVSPANASNKNVTWSS
                     SNTSVATVSSSGVVTGVSAGNATITVTTVDGGKTDTANVTVANAPPTQSETTTYTFST
                     EGWKATPANWQSMYPANGFDTDQNYLRGMQVLGSNTAYGNSPYFDNVKKVIFGTAASG
                     SGAGTYSAYLVTSSTSNLKDGTQIGTDVTVSKPGATIIERTFELTTPASGHVQAIIDA
                     TSNSLYLKYVTIEVATPINYQGEADLWANGFLESTSAGCSSQSQSTLSSIWPNLSNEY
                     NALAAEVKAIIEGSTPNSAGSEVEQALARYVFIVNKYGFTAFINGNPASGVHDTMHND
                     KQLAEPLLILFSVATLSLIPFVY"
BASE COUNT          676 a          383 c          452 g          605 t
ORIGIN      
        1 aattttacta tctgcgctct ttgcttttag tagtgtacgc gctagtagta atatagttag
       61 aacagaagcg tggagcggga cacaaactag tactgaaggt acatattact ccaccgttgg
      121 tagtgaaacg ggcacagctt tacttaataa attaaagaca attacgggta atccgactcc
      181 gcaaactagt tatgattggt atcgctatga agcagcggat gaggcggaaa atgagagcaa
      241 taaagtgctt ttaatttatt cgcgacaaaa agtacttaaa acggcccatg tttcaggaag
      301 tacaggttgg aatagagaac atacttatcc tgactccaaa atatcgggtg ctgccgaatc
      361 ggacaaccat catattttcg ctgatgacaa tcgcacaaat agtactcgtg gcaacatgcc
      421 atttggtgtt gttgctcaaa ctagcacaaa tcaggttaaa gacggttatg gaaatttgac
      481 agattgctat aaagatggta cttattttta tccaaacgaa cccgctcgtg gtgaagtagc
      541 gcgcgctacg atgtatgtta atatgcgtta tggttatagt gtgactggta acttccaaag
      601 tattgcactt atgctagagt ggcatttaca aaatcctgta tcaaatcgcg aaatatatcg
      661 caataatacc gttcatacaa tgcaaaaaaa tcgtaatcct tttattgatc atccagaata
      721 tgcatgtcgg atttggggta acactaatag tgaaacacaa gctatttgcg gggctgcaca
      781 acaagtcgat gttactagtg taagtgtggc accgtcaagt ggtagcatta acttattgga
      841 tgttaatgag acattacaat taagcgcgaa tgttttaccc gctaacgcta ctaataaaag
      901 tgttaactgg agcacttcaa actcaagtat tgcgactgtt gacgctaacg gtttagtcac
      961 gggtaaagca gtgggaaacg tcacaattac tgctacttcg gtatcaaatc catcagttaa
     1021 aggtacagca tctattgctg ttagtaatac atcaattcct gttactggtg tcactattag
     1081 tgataaatcg ttcacattag ttaaagacgc tactaaaaca ctaactgcga cagtaagtcc
     1141 agcgaatgca tcaaataaga atgtaacatg gtcatcatca aatacaagtg ttgcgactgt
     1201 ttctagtagc ggggtcgtta ctggagtaag cgcaggaaac gccacaataa ctgtcacaac
     1261 agttgatggt ggtaaaactg atactgcaaa cgtaacagtc gccaatgcac cgccaaccca
     1321 aagtgaaaca acaacttata cattttcaac cgaaggttgg aaagctacac ctgctaattg
     1381 gcagagtatg tatcctgcta atggttttga cactgatcaa aactatttaa gaggtatgca
     1441 ggttttaggt agtaatacag cctatggtaa tagtccgtat ttcgataatg tgaaaaaggt
     1501 tatttttgga acagcagctt caggtagtgg ggcaggaact tatagtgctt atctagtaac
     1561 ttctagtacc tcaaacttaa aagatgggac acagattggt accgatgtaa ccgttagtaa
     1621 accaggcgcg actattattg agcgtacttt tgaattaaca acaccagcgt cgggtcacgt
     1681 tcaagctatt attgacgcga cttctaattc tttgtactta aaatatgtaa cgattgaagt
     1741 agcgacaccc attaattatc aaggggaggc tgatttatgg gcaaatggct tcttagaaag
     1801 tacaagtgct ggttgtagta gccaatcaca aagtacatta agtagtattt ggccaaattt
     1861 aagtaatgag tataacgcat tagcagcaga agttaaagca attattgaag gctcaacacc
     1921 aaactctgct ggtagtgaag ttgaacaagc tttagcgcgt tatgtgttta ttgttaataa
     1981 atatggattt actgccttta ttaatgggaa ccccgctagt ggtgtgcatg acacaatgca
     2041 taatgataaa caattagcgg aaccgctttt aattttgttt agtgtagcaa cgcttagctt
     2101 aatcccgttt gtgtat
//
LOCUS       DAOSEX010000146         2114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_52101_length_2114_cov_2.785333, whole genome shotgun sequence.
ACCESSION   DAOSEX010000146 DAOSEX010000000
VERSION     DAOSEX010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52101_length_2114_cov_2.785333"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /locus_tag="PK612_03185"
     CDS             complement(<1..1859)
                     /locus_tag="PK612_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOH95145.1"
                     /translation="MNDLVKRLKGYDYVIVDYATKLELLKLRIRNPLLSFKIFTVEEL
                     ITKFLGSASDQSLFALLEKYNNLTYEHAKIINNTIVFPNVSKSSLKVNTLSTYQKALK
                     ALNLLESDFLIAHSLHNKKILLPKYLASNYLLINLLNSVHANFEFTEDMHNFIHEITV
                     FHTGVDEVYAALNRIAALLANGVKPSLIKILKPNSAYEELLRKLAPQFNIVLGPRLFP
                     LNAFSDVVAFVEQFKGQRIDISDPLIQTLRMSTSAPLIALYNLIIAIDFNSVSNKHHY
                     AILKDKIKSESIALEDNDGIEFINEIPLFSANDTYYFYLNFANGAAPNIQSLNPYLNN
                     EERKQLGLFDDETIALINENKLISTLNNNKNIYISFAKIFGQENYELSTLADKLEYEC
                     IEHQLAHKIYSQKYFNYLTGVSYDKLIFYKKHDENFDELFIANDYFDDYGSFDYKFKG
                     INHYHEMVYLSPTKIDLYQRLPFDYFVQHLLNIVDDRPSIHLHFGEFVHSVLENSTSE
                     ETFKVCFSHYLDQMNFNTKDRFFVENERDLIAEVFRFNQRYLNTVMPTKIHNETLINA
                     PFAAKCVLNGRLDRILLFEKDEKKYIFVVDFKTGNATTNPKNYEYGLDLQLPL"
BASE COUNT          728 a          326 c          286 g          774 t
ORIGIN      
        1 taaagcggta attgtaagtc taaaccatat tcataatttt taggattagt ggttgcattc
       61 ccagttttaa agtcaacaac aaatatatat ttcttttcat ctttttcaaa taataaaata
      121 cgatctaatc gaccatttaa tacacatttt gcagcaaaag gggcattaat tagtgtttca
      181 ttatgaattt tagtgggcat aactgtattt aaataccgtt gattaaatct aaaaacttcg
      241 gcaattagat ctcgctcatt ttctacgaaa aaacgatctt tagtattaaa attcatttgg
      301 tcaagatagt gactaaaaca tactttaaat gtttcttcac tcgtactatt ttctaataca
      361 ctatggacaa attctccaaa atgaagatga atgcttggcc ggtcatctac gatattaagt
      421 aaatgttgta caaaataatc aaatggtaat cgttgatata aatcgatttt agtgggtgat
      481 aaatatacca tttcatggta atgattaatc cctttaaact tataatcaaa acttccataa
      541 tcatcaaaat aatcattagc gataaataat tcatcaaagt tttcgtcatg ttttttataa
      601 aagattaatt tatcgtaaga aacacccgtt aaataattaa aatacttttg actataaatt
      661 ttatgcgcta attggtgttc aatgcactca tattctagtt tatcggctaa tgtacttaat
      721 tcgtaatttt cttgaccaaa tatcttagca aatgatatat aaatattctt attattgttt
      781 agagtactta ttaatttatt ttcattaatt aaagcaattg tttcgtcatc aaataaacct
      841 aattgtttgc gttcttcatt atttaaatat ggatttaagc tttgaatatt tggcgcagca
      901 ccattagcga aattaagata gaaataatag gtgtcgttag cgctaaataa cggtatttca
      961 ttaataaatt caatcccgtc attgtcctct aaagcaattg actcactttt aatcttatct
     1021 tttagaatag cgtaatgatg cttattacta acactattaa aatcaatagc aatgattaaa
     1081 ttgtagagag caattaatgg tgcacttgta ctcatccgca gtgtttgaat aagtgggtca
     1141 ctaatatcta ttcgctgtcc tttaaattgt tcaacgaatg caactacatc agaaaaggca
     1201 ttaagtggaa atagccgggg tccaagcaca atattaaact gtggcgctaa ttttcgtaat
     1261 aattcttcat atgcactatt tggtttcaaa atcttaataa gcgaaggctt cactccattt
     1321 gctagtaaag ctgcaatcct atttaaagca gcataaactt catcaacacc ggtatgaaaa
     1381 actgtgattt catgaataaa attatgcatg tcctcagtaa attcaaaatt agcgtgaacg
     1441 ctgtttaaca aattaattag taagtagtta cttgctaaat attttggtaa taaaattttc
     1501 ttattatgta aactatgagc gattaagaag tcactttcta ataaatttaa agctttcaag
     1561 gctttttgat atgttgataa tgtattaact tttaaactac ttttacttac attaggaaaa
     1621 acaatcgtgt tattaattat ctttgcgtgt tcataagtta gattattata tttttcaagt
     1681 agggcgaaaa gactttgatc actagcactc cctaaaaatt tcgtaattaa ttcttcaact
     1741 gtaaatattt taaaagataa aagtggattt cttatacgca actttaaaag ttcaagtttt
     1801 gtcgcataat caacaataac atagtcatag ccttttaaac gcttaaccaa atcattcata
     1861 tatttgatta tagcaaagta agttaagacc cccaatactt tttctatagt tttattaact
     1921 tttgttaata taacttagaa gaaataaaaa acgctggctt attaaccagc gtaattaata
     1981 gtttaatgtt actcttcttc gctgaaggtt aatacagcgc gtacagcctt tttccatcca
     2041 gcatattttc gttcccgaac tttctcctcc atttttggtt cataagttcg tgtgtactca
     2101 tgaatctttg taat
//
LOCUS       DAOSEX010000147         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_52375_length_2107_cov_2.230019, whole genome shotgun sequence.
ACCESSION   DAOSEX010000147 DAOSEX010000000
VERSION     DAOSEX010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52375_length_2107_cov_2.230019"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PK612_03190"
     CDS             complement(<1..556)
                     /locus_tag="PK612_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOH95146.1"
                     /translation="MANKNTAKRYELPLIITRNQVYFPFNQSDDLDAGRPATVEAIKY
                     ANNSDDKLLIVATQKVFTVNNPSMNDIYEVGVLARLTKVRERPHHITVQLEPLARVFI
                     NELDESGPFIIADATVTFHEPLDPDANPERLKNALFDFFNDKLELTRRLHIDLDALKA
                     ADMNLEELIYFLANKTLNSTEQRMA"
     gene            complement(600..1874)
                     /gene="tig"
                     /locus_tag="PK612_03195"
     CDS             complement(600..1874)
                     /gene="tig"
                     /locus_tag="PK612_03195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOH95147.1"
                     /translation="MKLKVTKLETSKYELEVEVSEAKWSAAREKAFKKLAKDVTVQGF
                     RKGKAPEHLVRAKIDPVKELDEAVHSLLDEAFQFAIKESGHYPVIQLTYDVVKVSDTE
                     LVLKFIIVAAPVVTLGQYEGLKIGRGEANVEDSAVEVRLNSILEQNAELILKEDAAAL
                     GDTVVLDFEGFVDGEAFEGGKATNHELELGSGQFIPGFEDALVGVKAGESRDVNVTFP
                     ENYHEQLAGKKAVFKTTVHEVKEKRLPALTDDFAASLNMPNVTDVASLRVHVLFELTK
                     QAEAAERNEYVEKILAKLRETSTFELANDIIDREVEHMDERLKNQVTQQGMEWKQYLE
                     LTKQTEEEIKERFVSEATVNLQNFLVLNEIAKAKEIEVTDAEVDFEIAMMAQQYNMEE
                     KRIRELLGDNINNLRNDVRQKKIINFLLENNE"
BASE COUNT          603 a          406 c          355 g          743 t
ORIGIN      
        1 cagccattct ttgttcggtg ctattgagtg ttttattcgc taagaagtaa ataagttctt
       61 ctaaattcat atcggcagcc tttaaggcat ctaaatcgat atgtaaccgt cttgttaact
      121 ctaatttgtc attaaagaaa tcaaataagg cattttttaa gcgttcagga ttagcgtcag
      181 ggtcaagtgg ctcatgaaaa gtaacagtag cgtcagcaat aataaaggga ccactttcat
      241 caagttcatt aatgaaaaca cgggcaagtg gttcaagttg tacggtaatg tgatgcggtc
      301 tttcacgcac ttttgttagt cgtgcgagta cgccgacttc ataaatatca ttcatcgatg
      361 ggttattaac cgtaaaaacc ttttgcgttg ctacaattag taacttatca tcactattat
      421 tagcgtattt aatcgcttca acagtcgccg gtcgtcccgc atctaagtca tcactttgat
      481 taaacggaaa atacacttga ttgcgtgtaa taataagcgg taactcatag cgttttgctg
      541 tatttttgtt agccatgtaa cctctccttt tctttatgta aaacttaact aattaataat
      601 tattcattat tttctaataa gaaattaata attttctttt ggcggacatc attacgtaaa
      661 ttgttgatgt tatcgccaag taattcacgg atgcgttttt cttccatgtt atattgttgc
      721 gccatcatcg caatttcaaa atcgacttcc gcatcagtaa cttcaatttc tttagctttg
      781 gcaatttcat taagcactaa gaagttttgt aagttaacgg ttgcttcact cacaaagcgt
      841 tctttaattt cttcttcagt ttgttttgtt aattctaaat attgtttcca ttccattcct
      901 tgttgggtga cttgattttt aagtctttca tccatatgtt cgacttcacg gtcaataatg
      961 tcattcgcaa gttcaaaggt tgatgtttcg cgtaatttcg ctaagatttt ttcaacatat
     1021 tcattgcgtt cagcggcttc ggcttgttta gtaagttcaa ataagacatg gacgcgtaaa
     1081 ctagcaacat ccgtaacatt tggcatatta agcgaggctg caaagtcatc agttaacgct
     1141 ggtaaacgtt tttctttaac ttcatggact gttgttttaa agacagcttt cttaccagcg
     1201 agttgttcat gataattttc ggggaaagta acgttaacat cacggctttc acctgctttt
     1261 acaccgacaa gtgcatcttc aaaacctggg ataaattgac cactgccaag ttctaattcg
     1321 tgattagtgg ctttaccacc ttcaaaagct tcgccatcga caaacccttc aaagtcaagc
     1381 acaacagtgt cacctaaagc agcagcgtct tcttttaaga taagttcggc attttgctct
     1441 aagattgagt taaggcgtac ttcaacggca ctgtcttcaa cattcgcctc accacgaccg
     1501 attttaagcc cttcatattg gccaagtgta acaactggtg cagcaacaat aataaattta
     1561 aggacaagtt cagtatcact tactttaacg acgtcgtaag taagttgaat tactggataa
     1621 tgtccgcttt ctttaatcgc aaattggaaa gcttcatcga gtaaactgtg cactgcttca
     1681 tctaactctt taacgggatc aattttagcg cgcactaagt gttctggtgc tttaccttta
     1741 cggaagcctt ggacagtgac gtcttttgct aatttcttaa aggctttctc acgtgccgca
     1801 ctccatttag cttcactaac ttcgacttct aattcgtatt tacttgtttc aagtttagtt
     1861 acttttaatt tcatatataa aaattcctcc atttataacc ttagtaagta tactaaaata
     1921 tcgctcattt tagtaataaa gtggcttatt tatgtaatat ttccgtgatt aaggacaatt
     1981 taatcatttt atttcgttaa atcataaaaa gacatgatta atcaaagtag gtaaagtgat
     2041 actttgtaaa tatcgcaaat agtgtttcag cgtttagacc gtgttcttca atatattcat
     2101 taacatt
//
LOCUS       DAOSEX010000148         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_52878_length_2092_cov_3.305842, whole genome shotgun sequence.
ACCESSION   DAOSEX010000148 DAOSEX010000000
VERSION     DAOSEX010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52878_length_2092_cov_3.305842"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /gene="thiI"
                     /locus_tag="PK612_03200"
     CDS             <1..925
                     /gene="thiI"
                     /locus_tag="PK612_03200"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOH95148.1"
                     /translation="VKQFALELILELKPKTFKVITKRKDKLFPLTSDEINRSVASHIL
                     KNAEIKVDVHNPELPLRLEIDKSRILVVGKRYDGLGGYPLGAAGKGLVLLSGGIDSPV
                     AAYLMIRRGIKIAAIHFAAPPYTSEEVLKKIEALTAKLNNYQPHIRLYVVPFTKLQLA
                     IYEHATSGYAITLMRRTMLRISTVIAKRHGYKVLVTGDSLGQVSSQTLESLVVINEVT
                     NYPIIRPLATLDKNEIIKIAKDIETYPISILPYEDCCTIFPVTNSVTSPKLEDAVFFE
                     SKFDYESLISEAIRDTKKVDIRLDDEKEDLF"
     gene            938..1069
                     /locus_tag="PK612_03205"
     CDS             938..1069
                     /locus_tag="PK612_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95149.1"
                     /translation="MERLTTKLPNILRVFDMGDALAFKEEEHYYIIIKRRSHLPIYN"
     gene            1205..1531
                     /locus_tag="PK612_03210"
     CDS             1205..1531
                     /locus_tag="PK612_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95150.1"
                     /translation="MNQSEIMKNLKQEIKSSYEGFLKLGIHDEQAFHEHKEAIAFSVH
                     TVKAIAKILEPIPNEILYFKGGIGDTLAAVKEYAKNLVSKFGTLNTYKLYDFMSIDLP
                     TLRDTL"
     gene            complement(1609..1968)
                     /gene="rplT"
                     /locus_tag="PK612_03215"
     CDS             complement(1609..1968)
                     /gene="rplT"
                     /locus_tag="PK612_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOH95151.1"
                     /translation="MARVKSSPVTRARRKKVLKQAKGYFGAKSLLFRTAKEQVMHSLR
                     YAYIDRRRIKRDYRKLWIKRINAGCRMCEISYSQFMHGLKLANVDINRKMLSELAIND
                     FETFKGLTETAKKALAK"
     gene            complement(1981..>2092)
                     /locus_tag="PK612_03220"
     CDS             complement(1981..>2092)
                     /locus_tag="PK612_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017549071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HOH95152.1"
                     /translation="TSHLAPRKTTKQKRQLRKARLVHKSDAKRIKYLIQN"
BASE COUNT          714 a          351 c          354 g          673 t
ORIGIN      
        1 agttaaacaa tttgcgcttg aactcatcct tgaactaaag ccaaaaacat ttaaagttat
       61 tactaaacgt aaagataaac tttttccatt aacgagcgac gaaattaatc gcagtgtcgc
      121 gagtcatatt ttaaaaaatg ctgaaatcaa agttgatgtc cataatcccg aattaccact
      181 tcgtcttgaa attgataaaa gtcgtattct tgttgtcggt aaacgttatg acggcttagg
      241 cgggtatccg cttggtgctg ctggaaaagg tttagtgctc cttagtggcg gaattgattc
      301 accagttgcg gcttatttaa tgattagacg cggaattaaa attgcagcaa tccactttgc
      361 ggctccacct tatacaagtg aagaagtatt aaagaaaatt gaagcactaa ccgcaaaact
      421 taataactat caaccgcata ttagattata tgtcgtacca tttactaaac tccaattagc
      481 gatttatgaa cacgctacaa gtggttacgc tattacctta atgcgacgca cgatgcttcg
      541 cattagtact gtcattgcta agcgtcacgg ttataaagta ctagtgactg gtgattcact
      601 tggtcaagtt tcaagtcaaa cacttgaatc actcgtcgtt attaatgaag tcactaatta
      661 tccaattatt cgcccactag cgacgctcga taaaaatgaa attataaaaa ttgctaaaga
      721 cattgaaact tatccaattt caattttacc ttatgaagat tgctgtacaa tattcccagt
      781 tacaaactca gtaactagtc ctaaactaga agatgcagtg ttttttgaaa gtaagtttga
      841 ttatgaatct ctcatcagtg aagcaattcg tgatacgaaa aaagttgaca ttagattaga
      901 tgatgaaaag gaagatttat tttaaaagaa agataatatg gaaagattaa ctactaaatt
      961 accaaatatc ttaagagtgt tcgatatggg tgacgcttta gcgtttaaag aagaagagca
     1021 ttattacatc atcattaaac gcagatctca cctcccaatt tataattgac gatatttcga
     1081 actcgtcatt aaaaatgttt gcgaaataaa gaatatgacg ctaatttctg ctcttgaaat
     1141 tgaagataat ccgtttaaag cttactttat agaaaaagga acactaatta agagggccat
     1201 ttaaatgaat caaagtgaaa taatgaaaaa cttgaaacaa gaaataaaaa gtagttatga
     1261 aggcttttta aaactaggta tccatgatga acaagctttt catgaacata aagaagcaat
     1321 agcctttagt gtgcatacag ttaaagcgat tgctaagata ttagaaccca tccctaatga
     1381 aattttatac tttaaagggg gaataggcga tactcttgcc gctgttaaag aatatgctaa
     1441 aaatctagtt agtaaatttg gcacacttaa tacatataag ttatatgact ttatgagtat
     1501 agatttacct actcttcgtg acactctata attattgaaa agtgaaacat taaaaaaggg
     1561 agtaaacata aatctactcc catttttatt aagaaagaaa tatttatatt attttgcgag
     1621 tgctttttta gctgtttcag ttaaaccttt gaatgtttca aagtcgttaa ttgctaattc
     1681 acttaacatt ttacgattga tatcaacatt tgcaagtttt aaaccgtgca taaattgact
     1741 ataactgatt tcacacatcc gacagccggc gttaatgcgt ttaatccata atttacgata
     1801 atcacgttta atccgacgac gatcgatata agcataacgt aaggagtgca taacttgctc
     1861 tttagcagtt ctaaagagta agcttttggc gccaaagtaa cctttagctt gttttaaaac
     1921 tttttttcta cgggcacgtg tgaccggtga actttttact cttgccatga ttccttcctc
     1981 ctaattctga attaaatact taatacgttt agcatcactc ttatgcacta aacgcgcttt
     2041 tcgtaattgt cttttttgtt ttgttgtttt gcggggggct aaatgcgatg ta
//
LOCUS       DAOSEX010000149         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_52906_length_2091_cov_11.136051, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000149 DAOSEX010000000
VERSION     DAOSEX010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52906_length_2091_cov_11.136051"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PK612_03225"
     CDS             complement(<1..464)
                     /locus_tag="PK612_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95153.1"
                     /translation="METKNINTKQLSIIVRIGIIFLFIIGLIMVIVWYPFTISLTIIG
                     PVDVEPTQLQKIQVYAQSAFYYLTSVPCFIILIFLWIIAGRLKKNGRFIAKDVRLLNA
                     ANIMLLIDLAIFLVGNIIIVFLEINFFVLAHFFIAVIGLVVLIFMWTTTLLI"
     gene            544..620
                     /locus_tag="PK612_03230"
     tRNA            544..620
                     /locus_tag="PK612_03230"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:578..580,aa:Pro,seq:ggg)
     gene            685..1179
                     /locus_tag="PK612_03235"
     CDS             685..1179
                     /locus_tag="PK612_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95154.1"
                     /translation="MKHTISEKLQFVKMHIEENVPISEIATNYGFDPTSLKYCCSLYR
                     MYGEKAFEKKEKARTYTREEKLKAIAIILSGEKTIREVALGMMLSDPKIISDWVNKYR
                     REGEAGIKDTFSRAAYLKHDDRLFVKEHKKLLEDLERTKAENEYLKKSFPHILKRSKQ
                     PKKK"
     gene            1194..2042
                     /locus_tag="PK612_03240"
     CDS             1194..2042
                     /locus_tag="PK612_03240"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOH95155.1"
                     /translation="MRGRYKLKMLLEIAGLSHQTYAYNDKHMDEQANKDKLIEDEIVS
                     IFNENYQKYGIPRITLELKSRGHHINHKKVERLMKKLKIQARPKQRKYRSYRGEVGRI
                     ADNLLNQDFTTNDFYEKIGTDVTVFIGRFGKVYVSPIIDFHNREVLAYDASVNPDYRQ
                     INRMFQRLKIMHGNEFKNTIMHSDQGWQYQMKKYREKLAELKIIQSMSRKGNCLDNSP
                     TENFFSRMKEEMFYGKEHLYETEEQLIRAIDEYIYYYNNKRIVVRLKMSPIQFKNLQQ
                     GTLLCI"
BASE COUNT          807 a          317 c          402 g          565 t
ORIGIN      
        1 ctaataagta atgttgttgt ccacataaaa attaatacga caagcccaat tacagcaata
       61 aagaaatgcg caagtacaaa gaaattaatt tctaagaaaa caataataat attaccaact
      121 aaaaatatcg ctaaatcaat taataacata atattagcag cgttaagcaa acgcacatct
      181 ttagcgataa accgaccatt tttctttaag cgaccagcga ttatccacaa aaatattaaa
      241 atgataaaac atggcactga tgtaagataa taaaacgctg attgcgcata tacttgaatc
      301 ttttgtaact gtgttggttc aacatcaact gggccgataa ttgtaagcga aatagtaaaa
      361 ggataccaga caataaccat aattaagccg atgataaaaa gaaaaataat accaatgcgg
      421 acaatgatcg ataattgttt cgtatttatg tttttagttt ccataacata attataacgc
      481 taaaatgcca caaatgtagt tatcactact aatttcctta aattacttta taatatttgt
      541 agtcgggacg tagcacagct tggtagtgcg ctaccttggg gtggtagagg tcgtgagttc
      601 gaatctcatc gttccgacca ttttattgaa acttccccca aaattttgct caaagagagt
      661 aaaataaaaa gggggaattt ttaaatgaaa cacacaatct cggaaaaact tcaatttgtg
      721 aaaatgcaca ttgaagaaaa cgtacctatt tcagagattg caacgaatta tggatttgat
      781 ccaacatcat taaaatattg ttgcagtttg tatcgcatgt atggtgagaa agcttttgaa
      841 aagaaagaga aagccaggac atacacacgg gaagaaaagc tcaaagcaat tgcaattatt
      901 ttaagtggcg aaaagacaat tcgcgaagta gcgttaggga tgatgctgag tgatcccaag
      961 ataatatctg attgggttaa taagtatcgg cgcgaaggag aagcggggat taaagataca
     1021 tttagtcggg cggcgtactt aaagcatgat gaccgtttat tcgtgaaaga acacaagaaa
     1081 ttattggaag atttagagcg aacaaaagcg gagaatgaat acttaaaaaa atcatttccc
     1141 cacatactca agagaagcaa acaaccaaag aaaaaatagc ggttgtgcga gcaatgcggg
     1201 ggcgttataa attaaagatg cttctcgaaa tagcgggttt atcacatcaa acatatgcat
     1261 ataacgataa acatatggat gaacaagcaa ataaagataa attaattgaa gatgaaatag
     1321 tctcaatttt caatgaaaac tatcaaaaat acggcattcc gcggataact ttggagttaa
     1381 agagccgtgg acatcatata aatcataaaa aggtggagag gttgatgaaa aaattaaaga
     1441 ttcaagctcg acctaaacaa aggaagtatc gatcataccg tggtgaggtt ggccgtatcg
     1501 cggataatct cttaaatcaa gacttcacga ctaacgattt ttacgaaaaa attggaactg
     1561 atgtcacagt atttattggt cgctttggaa aggtttatgt atcaccgatt attgattttc
     1621 acaaccgaga agttcttgct tatgatgcat cggtgaaccc agattatcgc cagattaatc
     1681 ggatgttcca acggcttaaa attatgcatg gtaatgagtt taaaaatacg attatgcata
     1741 gtgatcaagg ttggcaatat caaatgaaaa agtaccgcga gaaactagct gaattaaaaa
     1801 taatccaatc aatgagtcga aaaggaaatt gccttgataa ctctccgaca gaaaatttct
     1861 ttagtcggat gaaggaagaa atgttttatg gtaaagaaca tttgtatgaa acagaagaac
     1921 aattaattag agcgattgat gaatacatct attactataa taataaaaga attgttgtaa
     1981 gactaaaaat gagtccaata cagttcaaaa atttgcaaca aggtaccttg ttgtgtatat
     2041 aatggtaggg gttaacaact cttatgcaaa aataaggggg aaggctcata t
//
LOCUS       DAOSEX010000150         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_53048_length_2088_cov_3.364978, whole genome shotgun sequence.
ACCESSION   DAOSEX010000150 DAOSEX010000000
VERSION     DAOSEX010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53048_length_2088_cov_3.364978"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PK612_03245"
     CDS             <1..900
                     /locus_tag="PK612_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH95156.1"
                     /translation="FALASYFRQLDMHNNPDSWRAYFAIIEPDFPFLQPVKPIIGFAN
                     FEYVLKDPLFSNALINTAILTIISVPLTIMISLLLAVSLNSIKPLRATYQTIFFLPYV
                     TNGIALGMVFNTIFSHEVGGIMNQFIQFFGGKPVSWIRPLWSPEGTIPLSKFSLGFAI
                     TVQSIWSGLAFKILVFMAGLASIDKQYYDAARIDGSSRSTIFRRITVPLLSPQILYIT
                     ITSFIGAFKAYQSVISIVGGGPIHFGGENRTMWITVVGYIYMNRNVEKNIPLAAAGSV
                     VLLIVILFITLIQMQVSKKRVHY"
     gene            916..1974
                     /locus_tag="PK612_03250"
     CDS             916..1974
                     /locus_tag="PK612_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH95157.1"
                     /translation="MTDKDKKVTEAVELTAEEEVKLSHIDDERFQPSYEIAKEYKTRA
                     IVVKIITYTFLTLFAFFMLLPFWFMISVSVKTPLEYQREVSTGLRLFTANPSFVNFRI
                     VLGMDLKDPLKEMNEALLHIVETSPERSFIPYFLNTIIVAVVSTVFTIVTTVLASFAF
                     ARLEFKGKNFLFAILLATMMIPGEMMLITNIQTAKWLGWENTYRALIFVHGVSVFYIF
                     YLRQTFQQIPNELFLAAKVDGYGEFSYLWRVMIPIGMPTIVTIIILNVMGSWNAFIWP
                     TLIAQGYNPTIQKLFKYNHTMRLVSNGLMSTFTGQFASYDTVKIAASMIVTAPLLLVF
                     VFFRKYIMSGVSRSGIKG"
BASE COUNT          586 a          414 c          402 g          686 t
ORIGIN      
        1 tttgcacttg caagttactt ccgacaactt gacatgcata ataaccctga ttcgtggcgc
       61 gcatattttg cgattattga acctgatttt ccctttttac aaccggttaa accaattatt
      121 ggttttgcga actttgaata tgttttaaag gatccgcttt ttagtaacgc gcttattaat
      181 accgctattt taacaatcat ttccgtccca ctgacgatta tgatttcact tttacttgcc
      241 gtatcgctta attctattaa gccgttacgc gcaacttatc aaacaatttt cttcctcccc
      301 tatgtcacta acggcattgc cttagggatg gtctttaata cgatcttctc gcatgaggtt
      361 ggggggatta tgaaccaatt tatccagttc tttggcggaa aaccagtatc gtggattcgt
      421 ccgttatgga gtccagaagg aacgatcccg ttatcaaaat tctccttagg atttgcgatt
      481 actgtccaat caatttggtc aggcttagcg tttaaaatcc ttgtctttat ggcaggttta
      541 gcaagtattg acaaacaata ctatgacgct gcgcggattg atggctcaag tcgcagcaca
      601 atctttagac ggattacggt tccactttta agtccgcaaa tcttatatat tacgattact
      661 tcatttatcg gggcatttaa agcttatcaa agcgttatct cgattgtggg gggcggacca
      721 attcactttg ggggcgaaaa ccgcacgatg tggattacgg ttgtcgggta catttacatg
      781 aaccgcaacg ttgaaaagaa cattccactt gccgcagcag gttctgtcgt cttacttatt
      841 gtaattttat tcatcacgct tattcagatg caagtctcga agaagcgggt gcactattaa
      901 taggagggta gcgtaatgac agataaagat aaaaaagtca cagaagcagt cgaattaact
      961 gccgaagaag aagtgaaatt atcacacatt gatgacgaac gctttcagcc atcctatgaa
     1021 atcgcaaaag aatataaaac gcgggcgatt gttgttaaga ttattactta cacattctta
     1081 acgctttttg ctttctttat gcttttaccg ttctggttta tgattagtgt ttcagttaaa
     1141 actccacttg aatatcaacg tgaagtgtcg actggattac gcttatttac ggcaaatcca
     1201 agttttgtta actttaggat tgtcttaggg atggatttaa aagatccact aaaagagatg
     1261 aatgaagcac tcttacatat cgttgaaact tcaccagaac gcagttttat cccgtatttt
     1321 cttaatacga ttatcgtcgc ggttgtctca acggtcttta caattgtaac aacggtgctt
     1381 gcctcgtttg cgtttgctcg tcttgaattt aaagggaaaa acttcttatt tgcgatttta
     1441 ctcgcaacga tgatgattcc tggcgaaatg atgcttatta caaatattca aacagcgaaa
     1501 tggttaggat gggaaaacac ttatcgcgca cttatttttg tccacggcgt tagtgtcttc
     1561 tacattttct acttacgaca aacattccag caaataccaa acgaattatt ccttgccgcg
     1621 aaagttgacg gctatggtga atttagctac ttatggcgcg tgatgattcc aattggcatg
     1681 ccgacaatcg ttacgattat tattctcaac gttatgggta gctggaacgc ctttatttgg
     1741 ccaacactta ttgcgcaagg ctataacccc actattcaga aattatttaa atataaccat
     1801 acgatgcgac ttgttagtaa cgggttaatg tcgacattta ctggtcaatt cgctagttac
     1861 gacaccgtta aaattgccgc atcgatgatt gttactgctc cgttactgct cgtctttgtg
     1921 ttcttccgta aatacattat gagcggtgtc tcccgcagtg gtattaaagg ttagtaatta
     1981 ctaatcgtta ttatataaac aagaaaggaa agagagatat gaaatttaga aaactcatat
     2041 ctctctttcc tttcttgttt atataataac gattagtaat tactaacc
//
LOCUS       DAOSEX010000151         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_53319_length_2081_cov_3.441264, whole genome shotgun sequence.
ACCESSION   DAOSEX010000151 DAOSEX010000000
VERSION     DAOSEX010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53319_length_2081_cov_3.441264"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PK612_03255"
     CDS             <1..418
                     /locus_tag="PK612_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH95158.1"
                     /translation="HDLGVVANMADRVAVMYAGRIVEYGKVEEIFYNPKHPYTWALLS
                     SIPDVDTKERLEAIPGTPPNMVYPPKGDAFAPRSKYAMAVDFIEEPPYFKVSETHYAR
                     TWLLDPRAPKVEMPKIVSERIRISLERAGKGGEFDA"
     gene            411..791
                     /locus_tag="PK612_03260"
                     /pseudo
     CDS             411..791
                     /locus_tag="PK612_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1326..2081
                     /locus_tag="PK612_03265"
                     /pseudo
     CDS             1326..2081
                     /locus_tag="PK612_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          727 a          370 c          425 g          559 t
ORIGIN      
        1 gcacgacctt ggtgttgtgg cgaatatggc ggaccgcgtc gccgtgatgt atgcgggccg
       61 aattgtcgaa tatggtaaag ttgaagaaat cttctacaat ccaaaacatc cgtatacttg
      121 ggcattactc tcaagtattc ctgatgttga tacaaaagaa cggcttgaag caattcctgg
      181 tacaccaccg aacatggttt acccacctaa gggtgatgct tttgccccac gttctaaata
      241 cgcaatggca gtcgacttta ttgaagaacc accgtacttc aaagtaagtg aaacgcacta
      301 cgcacggaca tggttacttg atccacgtgc accaaaagtc gaaatgccaa aaatcgtttc
      361 cgaacgaatt cgcatttcac ttgaaagagc ggggaaagga ggagagtttg atgcttaaaa
      421 aacgtaaaag acgtgaagta acatttaaac aagaacttgt cgatcaagat attattctta
      481 gtgtacggca tttaaaacaa ttctttactt cgggacgcgg tgctaataaa ttacgcacaa
      541 aagctgttca tgatatttcc tttgatatta aaaaaggtga agtctttggt ttagtcgggg
      601 aatctggctg tggtaaaaca acaaccgggc gttcgattat taaactatat gacgtcacaa
      661 gtgggagcat ttattttaaa ggctaccgta ttaatgctgg ttcacgctgg aacgaaaaag
      721 aaattaaatg gaaaacaatt cgcactagac gtgaaattaa acggctgcgt aaagtattac
      781 gcgctgatat taagcgggaa aaagctgaac ttaaacaaca aattaaagat gctgaacttg
      841 atattttagc aagaagttca ccagaactag ccgctgctaa gcgtgagttt gctgcccttt
      901 tagtgacaat tagagaacgc aaaaatacat ttgccactga tgaatataac tataaaacta
      961 aagttcgtga cttaaaacaa gcacttaaag aagaactccg tgaccgtagt gacgatagtc
     1021 aagatgataa tgatattatc gttgcggaaa ttaataaaaa gtatgaatgt caacttaaac
     1081 gtgaaacaaa taagtttgaa ataattaaaa aagcttttgt taaggaacat gataaagata
     1141 gtaaacgtgt tttagaatta aaagcagtta ttaaagctgg tgataaaaat ctttcaccaa
     1201 cagtcaaaga agaacttgcg gcagagacaa aagtatttgc cgttaaagca aaagaagctt
     1261 atgacttgcg tgttagtgaa cttaaaacag caacaaacga agttattgct gacttaaagg
     1321 caaaacttgc ggatatcgtc tcgacacaaa aagaaaagat tgcacaaatt aactatgata
     1381 tgaaacatgt tgatacaagt atcatgaata agatgcaaat gattttccaa gatccaattg
     1441 atagtcttaa ccctcggatg actgttgaag atattattca agaaggtctc caaattcaag
     1501 ggatgaaaga ccgcgctgaa aactcgaaga aagttatcga atccttagaa aaggttggat
     1561 taattagcga ctatgcttcg cggtacccgc acgaatttag tggtggtcaa cggcaacgga
     1621 ttgggattgc tagagcgctg attatgaatc ctgaattcat tattgccgac gaaccagtca
     1681 gtgcgcttga cgtttcgatt cgcgcacaaa ttattaactt acttaacgac ttacgtgatg
     1741 aacttggctt aacgattctc tttatcgctc acgacttaag tgttgttaaa tatttctgtg
     1801 accggattgc agttatgtat tatggtaata ttgttgaatt aacaacgagt gatgagttat
     1861 ttaaacaccc attacatcca tatacaaaag cactcctaag cgccattcca aaaccagatc
     1921 cttatagtga aaaaacccgt gaacggattt tatataaccc gtttaccgct cacgattatc
     1981 gtgaagaaaa accaacacta caagaaatct taccaggtca ctttgtctta gcaaatagtg
     2041 aggaaatgga acgttatcgc gaagaaatta aagggttaga t
//
LOCUS       DAOSEX010000152         2077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_53474_length_2077_cov_6.209693, whole genome shotgun sequence.
ACCESSION   DAOSEX010000152 DAOSEX010000000
VERSION     DAOSEX010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53474_length_2077_cov_6.209693"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PK612_03270"
     CDS             complement(<1..884)
                     /locus_tag="PK612_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95159.1"
                     /translation="MERELQTIQLRKKSLTNRLMSVKNKAILTIYDSIVNERKDLHAR
                     LKKTTANDRFLFTPTVMAAKQTINEVKRLKLAQPQQDRERGEWLAILAFQAINKRQLS
                     KKISKKVYEYTREYEGEQKEDLLLKTAEINRSEQADDPKIFYICSRHNDCAVDHKDYQ
                     GKVYIDANWREVVKDDKEILKIEQYIRNFGVDTYQWVIFEPVWLVVRPNCRHYFKDLR
                     VSEVLGNKVENILKQNRMIHAKGRRSMRQTIRHDMTRKTYTINEVEALLSKYRERLSL
                     HEQMAAIKHTHILQKAID"
     gene            complement(811..>2077)
                     /locus_tag="PK612_03275"
     CDS             complement(811..>2077)
                     /locus_tag="PK612_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95160.1"
                     /translation="DKELERIIYDMLEDNDFIRMVNQEQLPLTLAQGWGAYKINIDEA
                     YEYPLIEYYEAENVRFIGKNRRIEGIIYLDYYELNNKKYVLFETRSIKRKTENVEAGS
                     YVEYNLFELKENNNIIPVELSTIKELSKLETIFIPGYMKILGVPTRIFHDPSDVNYGR
                     SILTGKIDLFDDLDQALSQASQTVKVSTPVEYYPVSVLEKNSFGVPILPVAYNRQFIK
                     KPASIPSGDGVMGSDTIQTSQPQLNFEQYAYEVKNKLDLILTGILSPATMGIDVAKKD
                     NAEAQREKEKITLMTRNNIINAQRKIIQDVVDVALDLLMYKNGQPISDERADFSVVYN
                     DFASPSFESKLQYLSPAYSGGAISTEKYVETLWGDMMSDEEQQEEVERLNKIRNADNL
                     TLEDFDGTGITDDSVEEEIFNEPLDVSKE"
BASE COUNT          519 a          462 c          346 g          750 t
ORIGIN      
        1 ttatcaatcg ctttttgtaa aatatgcgtg tgtttaatag ccgccatttg ttcatgcaat
       61 gataaccttt cacgatattt acttaacaac gcttctacct cgtttatcgt ataagtcttg
      121 cgtgtcatat cgtgcctgat ggtttgccgc attgacctcc gcccctttgc gtgaatcatc
      181 cgattttgtt tcagtatgtt ttccacttta ttccctaaca cctcactcac tcgcaagtcc
      241 ttaaagtaat gccgacaatt tgggcggact acaagccaca ccggttcaaa aataacccat
      301 tggtaagtat caactccaaa gttgcggata tattgttcta tttttaatat ctctttatca
      361 tccttaacca cttcccgcca gttagcatcg atataaactt tcccttgata atctttgtga
      421 tcaaccgcac agtcattatg gcggctgcat atataaaata tttttggatc atccgcttgc
      481 tcactgcgat ttatttcggc agttttaaga agcaagtcct ctttttgctc gccctcatat
      541 tctctcgtgt attcatagac tttttttgaa atctttttcg agagttgtct tttgttgatg
      601 gcttgaaacg ctaaaatcgc caaccactcg cctctttctc ggtcttgttg cggttgcgct
      661 aattttaatc tttttacttc gttaatggtt tgtttagccg ccattacagt cggtgtgaat
      721 aaaaacctat cattagcagt tgttttcttt aatcgagcgt gtaaatcttt ccgttcgttt
      781 acaattgaat cataaatcgt taatatcgct ttattcttta ctgacatcaa gcggttcgtt
      841 aaagatttct tcctcaactg aatcgtctgt aattcccgtt ccatcgaagt cctccagtgt
      901 cagattatcc gcattccgta ttttattaag tcgctcgact tcctcttgtt gttcctcatc
      961 actcatcatg tcaccccata aagtttccac atacttttca gtcgagattg ctccgccaga
     1021 atatgcagga gataagtatt gaagtttgct ttcaaaactt ggacttgcga aatcattata
     1081 tacaacgcta aagtcggctc tttcatcgct gataggttgc ccgtttttat acattaataa
     1141 atctaatgcg acatcgacca catcttgaat aatctttctt tgggcgttaa tgatgttatt
     1201 acgagtcatg agcgtgattt tctctttttc tcgttgagct tccgcgttgt cttttttcgc
     1261 cacatctatc cccattgtcg cgggacttaa aatacccgtt aaaattaaat caagcttgtt
     1321 tttcacctca taggcatatt gctcgaagtt taattgcggt tggctcgttt gaattgtatc
     1381 agaacccatc acaccatcgc cacttgggat actcgctggt ttcttgatga attgccggtt
     1441 atacgcaacc ggtagaattg gtactccaaa ggaattcttt tctaatacac tcaccggata
     1501 atattcaact ggtgtagaca ccttaaccgt ttggctggct tgacttaatg cttgatcgag
     1561 gtcatcgaat aaatcaattt tacccgttag aattgaccgc ccataattga catcgcttgg
     1621 atcgtggaat atccttgtcg gcactccgag tattttcata tagccgggaa taaagatagt
     1681 ttcaagtttt gataattctt taattgtaga taactccacc ggtatgatgt tgttgttttc
     1741 ttttagttca aataagttat attcgacata agaccccgct tccacatttt cagtcttgcg
     1801 ttttatcgag cgtgtttcaa ataatacata ctttttatta ttaagttcat agtaatcaag
     1861 gtagataatg ccctctatcc gtctgttctt gccaatgaaa cggacattct cggcttcgta
     1921 atattcaatt aaaggatatt catacgcctc atcgatgttt attttatacg ccccccaccc
     1981 ttgagcgagt gtcaaaggta attgttcttg attcaccatg cgtataaaat cattgtcctc
     2041 gagcatatca taaattattc tctcaagttc tttatca
//
LOCUS       DAOSEX010000153         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_53705_length_2071_cov_2.937996, whole genome shotgun sequence.
ACCESSION   DAOSEX010000153 DAOSEX010000000
VERSION     DAOSEX010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53705_length_2071_cov_2.937996"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2071
                     /gene="uvrA"
                     /locus_tag="PK612_03280"
     CDS             <1..>2071
                     /gene="uvrA"
                     /locus_tag="PK612_03280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOH95161.1"
                     /translation="ALNTIYNEGQRRYVESLSAYARQFLGNLAKPDVDSIEGLSPAIS
                     IEQKSTSNNPRSTVGTITEIYDYLRLLYARIGMPYCPQHDIPISSSSPEQILNKIFEY
                     PEKTRIQILAPLVKAEKGTHKDTLAKIAKEGFVRIRINGEIKALEEVGNLDKNKRHNI
                     DLVINRVVLKAENKSRIFEGINTALDYGHGYVIILFNNEETLFSTHNACHICGFSVPR
                     LEPRLFSFNAPMGFCPECRGLGLKREVDINILVPDKNVSLAKGAIEYYKNIVGTKNIE
                     WQEFEKLCELYNIPMHVPFKELSDKHKEIALFGSPNEHNFKIITGSGNTMYRRGKIEG
                     VKNKIERLYEETTSEFMRQYYEKFMRDVTCSTCNGKRLNEHALAVYINGKNIHDLTML
                     QIGELKQFLNETRPKLSVTDRTISQLVLDEIDHRLTFLVKVGLDYLTLARSAATLSGG
                     EAQRIRLATQIGSKLTGVLYVLDEPSIGLHQRDNNKLIQALRDMVDLGNTLIVVEHDE
                     ETIRSADHIIDIGPGAGIHGGEVVVTGTVEDVINCKKSITGDYLSGRKVIPLPPRRMP
                     GNGNYLKIYGAAANNLRHIDVSIPLNTLTLVTGVSGSGKSSLVEEVLFKAVDGHLRRV
                     ETYPGAYSKIEGLEHIDKVINIDQDPIGRTPRSNPATYTKVFDDIRELFAQTVDARAR
                     GYTKSQFS"
BASE COUNT          686 a          347 c          455 g          583 t
ORIGIN      
        1 agcgttaaat acaatatata acgaagggca aaggcgttac gttgaaagct taagtgctta
       61 cgctcggcaa tttttaggga atctcgctaa gcctgacgtt gattcaattg aagggcttag
      121 tcccgcaatt agcattgaac aaaagtcaac gagtaataac ccgcgttcaa cagttggaac
      181 aattacggag atttatgact acttacggct tttatatgca cggattggaa tgccttactg
      241 tccgcagcac gatattccaa ttagtagttc ctcacccgaa caaatcttaa ataaaatctt
      301 tgaatatcca gagaaaacaa gaatccaaat tttagcgcca cttgtcaaag ctgaaaaagg
      361 tacacataaa gatacactag caaagattgc aaaagaaggg tttgtgcgga ttcgaattaa
      421 tggtgaaata aaagcacttg aagaagtagg aaaccttgat aaaaataaac gtcataacat
      481 tgatttagtc attaatcgtg ttgtgctcaa agccgaaaat aaatcacgca tctttgaagg
      541 aattaatacc gcgctagatt atggacatgg gtacgttatt attcttttta ataatgaaga
      601 aacacttttt tctacgcata acgcgtgtca catctgtggt tttagtgttc cgcgtttaga
      661 accgcgacta ttcagcttta atgccccaat gggtttttgt ccagaatgtc gcgggttagg
      721 acttaagcgt gaagttgaca tcaacatttt agtgccggat aaaaatgtaa gtcttgctaa
      781 aggggcgatt gaatattata aaaatattgt cggtactaaa aatattgaat ggcaagaatt
      841 tgaaaagtta tgtgagctct ataacatccc aatgcatgtg ccatttaaag aattaagtga
      901 taaacataaa gaaatagcgc tttttggtag tccaaatgaa cataacttta agatcattac
      961 tggtagcggc aatacaatgt atcgacgcgg aaagattgaa ggcgttaaaa ataagattga
     1021 acggctttat gaagaaacaa caagtgaatt catgcgtcaa tattatgaga agtttatgcg
     1081 tgatgttact tgttcgacat gtaacgggaa gcgtcttaac gaacacgctc tagcggtgta
     1141 tattaacggt aagaacattc acgacttaac aatgttacaa attggtgaat taaagcaatt
     1201 cttaaatgaa acgcgaccaa aactaagtgt gacagatagg acaattagtc aacttgtact
     1261 tgatgaaatt gatcaccgtt taactttctt agtaaaggtc ggcttagatt acttaacact
     1321 tgcgaggagc gctgcgacgc tttcaggtgg cgaagcacag cgcataagac ttgcgacgca
     1381 gattggtagt aaattaaccg gtgttttata tgtgcttgat gaaccaagta tcggtcttca
     1441 tcagcgtgat aataataaat taatccaagc attacgcgac atggttgatt tagggaatac
     1501 actaattgtt gtcgagcacg atgaagaaac aattagaagc gctgatcata ttattgatat
     1561 tggtccagga gcggggattc atggtggtga agtagtggta acgggcactg tggaagatgt
     1621 gattaattgt aaaaaatcaa ttactggtga ttatctctcg ggccgtaaag taattccgct
     1681 accgcctaga cggatgcctg ggaacggtaa ttacttaaaa atatatggcg cagcggcgaa
     1741 taacttaagg catattgatg taagtatccc gcttaatacc ttaacacttg tcacgggtgt
     1801 gagcggttca ggcaaatcaa gtttagtgga agaagtactt tttaaggcag tggacggtca
     1861 tttacgtaga gttgaaactt atccaggtgc ttactctaaa attgaaggct tagaacatat
     1921 tgataaagta attaatattg atcaagaccc aattggtaga acaccgcgca gtaaccctgc
     1981 gacttatacg aaagtgtttg atgacattcg tgaacttttt gcgcaaactg tggacgctag
     2041 agcgcgaggt tacacgaaga gtcaattttc g
//
LOCUS       DAOSEX010000154         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_53813_length_2068_cov_4.054645, whole genome shotgun sequence.
ACCESSION   DAOSEX010000154 DAOSEX010000000
VERSION     DAOSEX010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53813_length_2068_cov_4.054645"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PK612_03285"
     CDS             complement(<1..690)
                     /locus_tag="PK612_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95162.1"
                     /translation="MADENKVTIEQEQNVVAEEVAKEETAPKTKKDKPLKVAGAQEKV
                     KKAPIKDKAKKAADTKPKQTKKVASKKSDTKVEKEKVDAKPKAEKAKKAKADPKKADA
                     KPETEKVAEKTLDTEAPAAKEKEPKEAPKKEAPKKAVPRRRRLSAMELRKREALKGYK
                     RFTKVRSRYYSFLNRTVKQSKPTTNLYNTIANKAPTLSGITRNEVKRFDPEFIEKIER
                     VIPSLEEIVVNP"
     gene            complement(698..>2068)
                     /locus_tag="PK612_03290"
     CDS             complement(698..>2068)
                     /locus_tag="PK612_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95163.1"
                     /translation="SFGFWGWFFFVILMILNVAVIGGLIFLLVLLIRRLVKYSSKNYD
                     KAKLIAEVQRLNKELFFAIQEKNEVLKLKAESLGLEDPSLVEGDKTSVVIDTFPKLGA
                     VDLKYKDKDTDFITPVLDREITLEQIAVRYRYFAASQLGLFYDIDLVRVLFAAMGTVK
                     IIILEGISGTGKTSLPYSLGKFFLNDVAICSVQPSWRDRTELIGYYNEFTKKFNESEF
                     LRAIYEASYRKDVNIVILDEMNLARIEYYFAEFLSIMEMPNPQEWYVEVTKTADEMNP
                     VQLVNGKLLIGQNIWFFGTANNDDSTFTITDKVYDRAISITLNERGQMFDAEFTEPLK
                     IPYEHLRRLFDEAQTNFPLSKANANKFSKLDEYVVNKFRIGFGNRILRQIHTFVPIYV
                     ASGGTEPEALDFMFASKILKKFRSLNLAFMRDELSALRAFIERTFGKNTFKHSLNQID
                     DFMTRA"
BASE COUNT          574 a          418 c          352 g          724 t
ORIGIN      
        1 cggattaacg acaatttctt caagagaagg aatcacgcgt tcaatttttt caataaactc
       61 tgggtcaaaa cgttttactt cattacgcgt aataccagac aaagttggtg ctttattagc
      121 aatcgtattg tataaattcg tcgttggctt actttgctta acagtccgat ttaaaaatga
      181 ataataacgg ctacgaacct tagtaaaacg cttgtagcct tttaatgctt cacgctttct
      241 taattccatc gccgaaagtc gtcgacggcg tggcacagct ttttttggtg cttctttttt
      301 gggcgcttct ttcggttctt tttctttagc ggcaggtgct tcagtatcaa gtgttttttc
      361 cgctaccttc tcagtttcag gcttagcgtc agctttttta gggtcagcct ttgctttttt
      421 cgccttttca gctttaggtt tagcgtccac tttttccttt tcaactttag tgtcactttt
      481 tttactagca acttttttag tttgttttgg tttggtgtcc gctgcttttt ttgctttatc
      541 tttaattggc gcttttttta ctttttcttg ggctccagcc acttttaaag gtttgtcctt
      601 ttttgtttta ggcgcagttt cttccttagc gacttcttcc gctacaacat tttgttcttg
      661 ttcaattgta actttattct cgtctgccat tatttaccta cgctctcgtc ataaaatcat
      721 cgatttgatt aagtgaatgt ttaaatgtgt ttttaccaaa tgtccgttca ataaaggcgc
      781 gtaacgcact taattcgtca cgcataaacg ctaagttaag tgatctaaat ttctttaaaa
      841 ttttggatgc aaacataaag tcaagtgctt ctggttcagt cccgccgcta gcgacataaa
      901 ttggtacaaa tgtatgaatt tgccgtaaaa tccggttccc aaatccaatg cggaatttat
      961 tcacaacata ttcgtcaagc ttactaaact tattagcgtt tgctttggaa agtgggaaat
     1021 tagtttgtgc ttcatcaaat aaccgccgta agtgttcata cggaatcttt aatggttcag
     1081 taaattcagc atcaaacatt tgtccacgtt cgtttaatgt aatactaatc gcacggtcat
     1141 agactttatc ggtaatcgta aatgttgagt cgtcattatt tgcggtacca aagaaccaaa
     1201 tattttgccc aattaataac ttaccattca ctaattgcac tgggttcatt tcatctgctg
     1261 tctttgttac ttcaacatac cactcttgtg ggttaggcat ttccataatc gataagaatt
     1321 ccgcaaagta gtattcaatt ctcgctaagt tcatttcgtc aagaataaca atgtttacat
     1381 ctttacgata acttgcttcg taaatcgccc gcaagaattc actttcgtta aacttcttcg
     1441 taaattcgtt atagtaacca atgagttccg tacggtcacg ccaggatggt tgcacactac
     1501 aaatcgcgac gtcatttaag aagaatttac ctaaggagta aggtaaagaa gttttaccag
     1561 taccagagat accttctaaa ataataattt taaccgtacc cattgcggca aagagcacac
     1621 gcactaagtc gatatcataa aataacccaa gttgcgatgc ggcaaaataa cgatagcgca
     1681 ctgcaatttg ttctaaagta atttcgcggt ctaataccgg tgtaataaaa tcagtgtctt
     1741 tatctttata ttttaagtcc actgccccaa gttttgggaa tgtatcaata acgaccgaag
     1801 ttttgtcacc ttccacaagt gaaggatctt caagacctaa tgattcagct ttcagtttaa
     1861 gtacttcatt tttctcttga atggcaaaga agagttcttt gttaagccgt tgtacttcgg
     1921 caataagttt cgctttatca taatttttgc ttgaatactt aacaagtcga cgaataagga
     1981 gcactaataa aaagattaag ccaccaataa cggccacatt aagaatcatt aagattacga
     2041 agaaaaacca accccaaaat ccaaatga
//
LOCUS       DAOSEX010000155         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_54301_length_2056_cov_3.091954, whole genome shotgun sequence.
ACCESSION   DAOSEX010000155 DAOSEX010000000
VERSION     DAOSEX010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54301_length_2056_cov_3.091954"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            405..905
                     /locus_tag="PK612_03295"
     CDS             405..905
                     /locus_tag="PK612_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95164.1"
                     /translation="MKKRIIAFYDVLIVAIISTPFTICSIFFFVFADTSNLEWLCKNW
                     YLIIFASLGVSLPVVGIFWITGYTLIMNNHYVFFYYFPHAKTWKKMINNIDIRWNQEL
                     FISEVLAVNIVKLTKEEKKHKVFYKHIRNKYLEIIIKNGGTKYVYVGNYAQCQIKKIM
                     KILLKE"
     gene            935..1459
                     /locus_tag="PK612_03300"
     CDS             935..1459
                     /locus_tag="PK612_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95165.1"
                     /translation="MIKVAGVISELIITFIPIYLISSLLLFPPLIFKDFIKFTGLTIL
                     VIVIVNIIFYIIAAIAKIFIKTAYIITSDSLITQINNEVHSTIYLKDVYYLTYNFGAI
                     DGQPSPTELVLFDKEHKYLISIENPPLLMVINIKKKCLNAKITYFNSRRILFFLVSSL
                     VISIIVSLISLIAN"
     gene            1706..1954
                     /locus_tag="PK612_03305"
     CDS             1706..1954
                     /locus_tag="PK612_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95166.1"
                     /translation="MEYKYNIDLKDVSFVEVQQRDYKFSSRGREKRGRGIETYRTGHK
                     FIEFNYLDGSVKRIHCNDLSRKQINKILEHCKNKKNYY"
BASE COUNT          755 a          251 c          327 g          723 t
ORIGIN      
        1 ttagtgatga ccatgaagtt attataactt attaactact gaaattacgg ccagacatat
       61 atttgatgaa gtacgaatag tatttaatgg tggaagcaat ctaaacacga ccatctacct
      121 agcggacatt atcgtaaaaa gttatccaat tgtaacaagt tatgaatatg atgaaaacga
      181 taatataatt aaaaaaacgc atggtattat ttcagcaaat aatgaatatg atgattatgg
      241 caacttagta aagcgctacg gacaaggtac aagtggagga gcatacgctt ctaatgaagg
      301 tggaagagta tttcaaacaa gttcgctcaa tcacggagta agaaataatg ccaaaaattt
      361 aataactggt caattaattg ataaaattgc cacataggag atttatgaaa aaacgaataa
      421 ttgcatttta tgatgtgctg attgttgcta tcattagcac accctttacg atatgttcaa
      481 tattcttttt tgtttttgct gatacaagca atcttgagtg gttatgcaag aactggtatt
      541 taataatctt tgcgtccttg ggggtttcac ttcctgtggt tggtattttt tggattactg
      601 gctatacatt aataatgaat aatcattatg tgtttttcta ttattttccg catgcaaaaa
      661 cgtggaaaaa aatgataaat aatattgata ttaggtggaa ccaagaactt tttatatcag
      721 aagtactcgc agtaaacatt gttaaattga cgaaagaaga aaaaaagcac aaggtatttt
      781 ataagcacat acgcaataaa tatttagaaa ttataatcaa aaatggtggc actaaatatg
      841 tatatgttgg taactatgct cagtgtcaaa taaaaaagat tatgaagata ttattaaagg
      901 aataggtttc atgaaaaaat ataagcggaa tataatgatt aaggttgcag gtgtaattag
      961 tgaactaata attactttta tcccaattta tttaatttct tcattattat tattcccccc
     1021 gcttattttt aaagatttta ttaagttcac tggtttaacg atattagtga ttgttatagt
     1081 caatatcatt ttctacatta ttgcggcaat tgcaaaaata tttataaaaa cagcttatat
     1141 aattactagc gattctttaa ttacccaaat aaataatgag gtgcacagca caatatatct
     1201 taaagatgta tattatctga catataattt cggtgcgata gatggtcaac caagcccgac
     1261 agagttagtg ctttttgata aagaacacaa gtacttaatt tcgatagaaa acccaccgct
     1321 attgatggtt atcaatatta agaaaaaatg tttaaatgca aaaataacgt attttaattc
     1381 gcgccgaatt ttattttttt tagtaagttc tttggtaatt tcgataatag tttcattgat
     1441 atctttaatt gctaattaaa tgtagtattg aagtgtgata aatgaaagat tatatatttc
     1501 cttttaattc gctaattaaa atgattattt cgttaattgt tttttgcttt ttgcgctctt
     1561 agatattttt ttgtggcgga caaaattctt ttttataatt ctaattccga tatcaatatt
     1621 tttaatatta tttgcaatca aaataattat tttaaaagag tcttctattt ttgtcagtgg
     1681 cgatttactt gtcggccttc ttaaggtgga gtataagtat aacattgatc ttaaagatgt
     1741 ttcttttgtt gaagtacaac aacgagatta caaattttct tcgcgcggcc gagaaaaaag
     1801 aggtagaggt atcgaaactt atagaacagg tcataaattt attgaattta attatttaga
     1861 cggttcagta aaacgaatcc attgtaacga tttatcacgt aaacaaatta ataaaatatt
     1921 agaacattgc aaaaataaga aaaattacta ttagataata tttagaagta tttggtacaa
     1981 tagcagcagg atatatttaa acaattacat attgaaaaag aataatattg aacttgataa
     2041 taagaacttt aaataa
//
LOCUS       DAOSEX010000156         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_54816_length_2044_cov_2.381599, whole genome shotgun sequence.
ACCESSION   DAOSEX010000156 DAOSEX010000000
VERSION     DAOSEX010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54816_length_2044_cov_2.381599"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PK612_03310"
     CDS             complement(<1..349)
                     /locus_tag="PK612_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95167.1"
                     /translation="MKLIKKLFALFSPLELVILMVSITISTLLVVPAFSKDTQKKTPI
                     LLFPTEKVYNTDGGRPIEVFDTSFFVVYEEVEEQTQNDIKAILNEYLISYHKLFDRHN
                     DYYAVEPHDNIHPT"
     gene            complement(336..890)
                     /locus_tag="PK612_03315"
     CDS             complement(336..890)
                     /locus_tag="PK612_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOH95168.1"
                     /translation="MSQVSNNGNKRKLSTQKLVRLAVFLALGVVFNIVENYITFIPAL
                     PGVKLGLANSIGLILLALYGPLEFFTIGLLRVLLSGTFSGFGTSFILSLSGFLLSSAF
                     VLLAYLFNKLSIYGLSLLSAVMHGVGQVVMIAIIYQNALMLNYLIIMTISGIISGLLI
                     ALLSKIVILRLKMFKEEECNYEIN"
     gene            complement(871..1284)
                     /locus_tag="PK612_03320"
     CDS             complement(871..1284)
                     /locus_tag="PK612_03320"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOH95169.1"
                     /translation="MKARKVKLNKTLFDTIIFSIVLVLTSATTLYLKRAFSTTEALYV
                     YVYVTDRLIDKQPLAIDNTFIYNEPEFLAPITLEIYNKKVRVEKEESPLNLCSIKGFT
                     DKVGDPVLCLPNSFYFVIMGFNYKEAPSPYVPSVE"
     gene            complement(1308..1622)
                     /locus_tag="PK612_03325"
     CDS             complement(1308..1622)
                     /locus_tag="PK612_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOH95170.1"
                     /translation="MQYFWGVVILLVIGALLGLLLAVASKAFAVKEDPRLEPITEMMP
                     GINCGTCGYPGCKELVVAMLKGEVKNLGQCRPLRPDAKAKIKEYLANHPDEEGNILTV
                     QG"
     gene            complement(1626..1808)
                     /locus_tag="PK612_03330"
     CDS             complement(1626..1808)
                     /locus_tag="PK612_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95171.1"
                     /translation="MSDVARIFVAIGVMAILIALYIGSYVLNKRTKKPEGCKDFASSC
                     SGCAVTSCSHNPLKEE"
     gene            complement(1810..>2044)
                     /locus_tag="PK612_03335"
     CDS             complement(1810..>2044)
                     /locus_tag="PK612_03335"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOH95172.1"
                     /translation="LDVVTTNGGVPVYNFLNMVGYAFFTGVGYAVILILFSFIQERLI
                     NNKNMPKSFQGSGIALVTIGLLVMAFLGFAGMI"
BASE COUNT          699 a          413 c          331 g          601 t
ORIGIN      
        1 ctgtcggatg aatattgtcg tggggttcaa ctgcataata atcattatga cggtcaaaaa
       61 gtttatggta actaattaag tattcattta gaatagcttt aatgtcattt tgtgtttgtt
      121 cttccacttc ttcatacacg acgaaaaaac tagtgtcgaa tacttcaatg ggacgcccac
      181 catcagtgtt ataaactttc tctgtcggaa ataataaaat cggtgtcttt ttttgcgtat
      241 ctttgctaaa cgctggaaca actaaaaggg ttgagattgt aattgaaacc attaaaataa
      301 ctagttcaag cggggaaaaa agcgcaaaga gttttttaat taatttcata attacactct
      361 tcctccttaa acattttaag tctcaaaatc acaatttttg ataagagagc aataagtaat
      421 ccgcttataa tcccacttat tgtcataatt attaaataat taagcattaa agcgttttga
      481 taaattatcg caatcatgac gacctggccg acaccgtgca tcaccgcact aaggagtgag
      541 agtccgtaga ttgataattt attaaaaaga taggctaata gtacaaaagc gcttgaaagt
      601 aaaaaaccac taagcgataa aataaaacta gtgccaaacc cactaaaagt cccgcttaat
      661 aaaactcgaa gtaagccaat cgtgaaaaat tcaagcggac cataaagtgc taaaagaatt
      721 aagccaatcg aattagcaag gcctaattta acacccggta aagccgggat aaatgtaatg
      781 taattttcaa caatattaaa taccacacct agcgctaaaa agacagcaag ccgcacgagc
      841 ttctgtgtcg ataattttct tttatttccg ttattcgaca cttgggacat acggtgaagg
      901 tgcctcctta taattaaatc ccataatcac aaaataaaaa gaattaggta gacaaagtac
      961 gggatcacct actttatccg taaaaccttt aattgagcat aaattaagcg gactttcttc
     1021 tttttcaacc cgtactttct tattgtaaat ttcaagtgta attggtgcta aaaattcagg
     1081 ttcattgtaa atgaatgtat tatcaatcgc taacggttgt ttatcaatta gacggtcggt
     1141 aacatagaca taaacataga gtgcttcagt agtagaaaag gcgcgtttta agtacaacgt
     1201 tgtcgcactt gttagcacca agacaatact aaagataatg gtgtcaaaaa gtgttttgtt
     1261 taacttaact ttacgcgcct tcatgtaaat aagagtaacc taacttttta tccttgaacg
     1321 gtaaggatgt tcccttcttc atccggatga tttgctaaat attctttaat ctttgctttt
     1381 gcatcaggac gcaacggccg acattgacct aaatttttca cttcaccctt aagcatcgcc
     1441 actactagtt ctttacatcc cggataacca caagtcccgc aattaatacc aggcatcatc
     1501 tccgtaattg gctcaaggcg tgggtcttcc ttgaccgcaa aagctttact cgcgacggca
     1561 agtaaaagtc ctaataaggc tccaataact aaaaggataa ctactcccca aaaatattgc
     1621 ataaattatt cctcctttag tggattgtga ctacatgatg tcaccgcaca tccgctacaa
     1681 ctactagcga aatctttaca accctctggt ttttttgtgc gtttatttaa cacataactt
     1741 ccaatataaa gggcaattaa gatcgccatc acaccgattg ccacaaaaat gcgcgcaaca
     1801 tcactcatct taaatcatcc ctgcaaatcc aaggaaggcc atcactagta aaccaattgt
     1861 tactaaagca atcccgctac cttggaaact cttcggcata tttttattat taattaagcg
     1921 ttcttgaata aaactaaaga gaattaaaat gacagcgtag ccaacaccag taaagaaagc
     1981 atagccaacc atatttaaga aattataaac tgggactccg ccatttgtgg ttacaacatc
     2041 aaga
//
LOCUS       DAOSEX010000157         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_54951_length_2040_cov_3.445340, whole genome shotgun sequence.
ACCESSION   DAOSEX010000157 DAOSEX010000000
VERSION     DAOSEX010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54951_length_2040_cov_3.445340"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1974
                     /locus_tag="PK612_03340"
     CDS             <1..1974
                     /locus_tag="PK612_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH95173.1"
                     /translation="LRGMIVDFSKTNPTDWDNVNFSVKKPTEIVPYELHVSDLTADET
                     WNGSEWKRKNFLGLIEKGTMYERNGLAVKTGFDHIKELGINALQILPFYDQENDERDM
                     EFNWGYNPKNYNVLEGGYSSDPYNGLVRIQEFKRVVQAYAEEDIRIIMDVVYNHVASI
                     SQHSFTKLVPGYYFRFNLDGSPRNSSGVGNDTASERIMMERYMVDSTYFWATEYKLGG
                     FRFDLMGLHTVEAMQSIMTKLKTYRDDIIVYGEPWNMESGEGMLSPKKPYAISSNLHQ
                     IAGLGGFNDQMRDAIKGGSNDTSRGWVQMAAGEIYDDRLNKIKDGLMGKTTGGSSDPT
                     QTVNYISVHDNLTIYDKLRRSGGDLFGAEQLKKQAVQAEALSLLSQGIGFVHAGSEIL
                     RSKPLPNGSYDHNSYKSPYTVNSIKWDEKFDNLAIHNLYRQMIALKVHAPAFSLNTRA
                     AIDNGAVTLHTPNNTTIKLTYSADGDTYTIYHFGSMGNTTVSDVKGQEVVFDSSGTAS
                     IGTVISANLRIPGNSTLITRNVRDRGANDLTMPDILDYEPFVPPVIEPDPYDEGSSSS
                     EISSSEPDTSDPSSSMPETTSLPDSSEESSTVTSDTSVSDTSLPPTSEDDGTTTKPDN
                     TKKAVIIVASSVGGTALVGAILFFVLKMLRKKP"
BASE COUNT          652 a          396 c          425 g          567 t
ORIGIN      
        1 ttacgcggaa tgattgttga ctttagtaaa acaaatccta ctgactggga taatgttaat
       61 tttagtgtta aaaaaccgac cgaaatcgtt ccttacgaac tgcatgttag tgacttaaca
      121 gccgatgaaa cgtggaacgg tagtgagtgg aaacgcaaaa atttcttagg cttaattgaa
      181 aaaggcacga tgtatgaacg aaatggtctt gcggttaaaa ctgggtttga ccatatcaaa
      241 gaattaggta ttaatgcctt acaaatttta ccattctatg accaagaaaa tgatgaacgc
      301 gatatggaat ttaattgggg ctacaacccg aaaaactata atgtactcga aggtggctac
      361 agtagtgatc catataacgg cttagtgcgt attcaagaat ttaaacgcgt tgtccaagct
      421 tacgctgaag aagatataag aattattatg gacgttgttt ataatcacgt cgcaagtatt
      481 tcgcaacaca gcttcacaaa actcgttcct ggttactact tccgctttaa tttagacggt
      541 tctccgcgta attcaagtgg tgttggtaac gataccgcaa gcgaacggat tatgatggaa
      601 cgctatatgg ttgattcaac ttacttttgg gcaactgaat ataaactcgg tggcttccgc
      661 tttgacttaa tgggcctcca taccgtcgaa gcgatgcaat caatcatgac taaacttaaa
      721 acatatcgtg atgatattat agtttacggc gaaccgtgga acatggaaag tggtgaagga
      781 atgctaagtc cgaaaaaacc ttacgccatt tctagtaatc tccatcaaat cgctggttta
      841 ggtggtttta atgatcaaat gcgtgatgca attaaaggtg gatcaaatga tacttcacgg
      901 ggctgggtgc aaatggccgc tggtgaaatt tatgatgacc gtttaaataa aattaaagac
      961 gggctaatgg gtaaaacaac tggtggctct agtgatccaa cacaaactgt taactacatt
     1021 tctgtccacg ataacttaac aatatatgac aaattacgcc gtagtggtgg cgacttattt
     1081 ggcgctgagc aacttaaaaa gcaagccgtt caagcagaag cactaagtct tttaagccaa
     1141 gggatcggtt ttgtacacgc tggtagcgaa atcttacgct caaaaccact accaaacgga
     1201 agttatgacc ataactcata taagagcccg tataccgtta atagtattaa atgggatgaa
     1261 aaatttgata acttagcgat tcacaattta tacagacaaa tgattgcctt aaaagtgcac
     1321 gcgcctgcct ttagtttaaa tactcgcgca gccattgata acggtgccgt cactttacat
     1381 acaccaaata atacaacgat taaattaaca tatagtgccg acggcgatac ttatacaatc
     1441 tatcactttg gctcaatggg taacactact gtcagtgatg ttaagggtca agaagtcgtc
     1501 tttgattcaa gcggcactgc aagcattggc acagttatta gtgcaaactt acggattcca
     1561 ggtaattcta cgcttattac tcgtaatgtc cgtgatcgtg gcgcgaatga tttaacaatg
     1621 cctgacatct tagattacga gccatttgtg ccacctgtta ttgaacctga tccctatgat
     1681 gaaggttcta gtagcagtga aataagtagc agcgaaccag acactagtga cccaagtagc
     1741 agtatgcccg aaactacttc attgcccgat agtagtgaag aatcaagcac ggttactagt
     1801 gacacaagtg ttagtgatac ttcattacca ccaacaagtg aagacgatgg cactacaaca
     1861 aaacctgata acaccaaaaa agccgttatc attgtcgcaa gtagtgtcgg tggtaccgct
     1921 cttgttggtg ctattctctt ctttgtgctt aaaatgttac gcaagaaacc ctagctaatt
     1981 acgactaaca aaaagcgacc gtgattggtc gctttttaac ttggataaaa taattggctc
//
LOCUS       DAOSEX010000158         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_55202_length_2034_cov_2.915109, whole genome shotgun sequence.
ACCESSION   DAOSEX010000158 DAOSEX010000000
VERSION     DAOSEX010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55202_length_2034_cov_2.915109"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /gene="era"
                     /locus_tag="PK612_03345"
     CDS             <1..664
                     /gene="era"
                     /locus_tag="PK612_03345"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOH95174.1"
                     /translation="EYLNKQAYQSIRGSEVTLLVVDASVEYGAGDEYLFSRLKVDHPL
                     IIVLTKIDLASPIHVQKVKEQWTQNYPDTPLIEVSSVRNFNIDALITLIFEHLEVGPL
                     YFPLPEDYAIRLENYINEIVREKIIMLTREEIPYSVAVVVDHLTESDTKVEAYLTIYC
                     EKDSQKGILIGKGAKMIKRIRLQAQYELSKFLGKDVLLELMVKVEPLWRSDPKILKKL
                     LG"
     gene            667..1374
                     /gene="recO"
                     /locus_tag="PK612_03350"
     CDS             667..1374
                     /gene="recO"
                     /locus_tag="PK612_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOH95175.1"
                     /translation="MEVVIIRLTPYREKDYIVNALSKEEGIITFRATGALNVKSKFAG
                     KLFLYALVDVELRESKAGHTLTNVESLTNIAKVFTDEKRIIALNIIGEVISKTLKDND
                     SISEAFPLIKKVLMALSETTKLISLVYLFTTNLLRILGSALVIDRCVNCGDTKSIIGV
                     SFNKGGLICKKCYDEKTITLNAEEIQMLRYGFMINEEELLRHEFSDEAALSLLTLMLT
                     YLEFTYNYKIMSKDLIK"
     gene            1447..>2034
                     /locus_tag="PK612_03355"
     CDS             1447..>2034
                     /locus_tag="PK612_03355"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232897.1"
                     /note="Catalyzes a two-step reaction, first charging a
                     glycine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOH95176.1"
                     /translation="MHKHSFEKIVNHLVTSGFVFQGSEIYGGLSNTWDYGPLGTSVKN
                     NISNLWWKKFVTESPYNVGIDAAILMNPKVWHATGHVTTFNDPLIDCRNCKARHRADN
                     LIEDQVKGLNVTGLPNEEMMKIIKEYGVKCTVCGKQDFTDIRQFNMMFKTHIGVTEDS
                     QSEVYLRPETAQGMFVNFKNVQRTMRKKLPFGIAGI"
BASE COUNT          700 a          294 c          409 g          631 t
ORIGIN      
        1 tgaatattta aataaacaag cttatcagag tattcgtggg agtgaagtaa cacttttagt
       61 cgtagacgct agtgtggagt atggtgcggg tgatgaatat ttatttagtc ggcttaaagt
      121 tgatcatcct ttaattatcg tcttaacaaa gattgattta gcttctccca ttcacgtgca
      181 aaaagttaaa gagcaatgga cacaaaatta tccggataca ccacttattg aagtaagcag
      241 tgtgcgtaat tttaatattg atgcacttat aacattaatc tttgaacatt tagaagtagg
      301 gccactttat tttccgttgc ccgaagatta cgcgattcgt ttagaaaatt atattaatga
      361 aatcgttcgt gaaaagatta ttatgttaac acgcgaagaa attccttata gtgtcgcagt
      421 tgttgtcgat catttaacag agagtgacac taaagttgaa gcttatttaa caatttattg
      481 cgaaaaagat agtcaaaagg gcattcttat tggtaaagga gcgaaaatga ttaaacgcat
      541 ccgtttacaa gcgcaatatg aattatcaaa gtttttaggt aaagatgtgt tactagaatt
      601 aatggttaaa gttgaaccgt tatggcgaag tgacccaaag atattaaaga aattattagg
      661 ctaaagatgg aagtagtcat tattcgttta acaccgtacc gggaaaaaga ttatattgtt
      721 aatgctttaa gtaaggaaga aggcattatc acttttcgcg ctactggagc acttaatgtt
      781 aaatcaaagt ttgcgggtaa gctcttttta tatgctttag ttgatgtgga gttacgcgaa
      841 agtaaagcag gacatacttt aacaaatgtc gaaagtttaa cgaatatcgc taaagttttt
      901 actgacgaaa agcgtattat cgctttaaat atcatcggtg aagtcattag taaaacacta
      961 aaagacaatg attcgattag tgaggcgttt ccgcttataa aaaaagtgtt aatggcgcta
     1021 agtgaaacaa caaaactaat ttcgttagtg tatcttttta ctacgaattt attacgcata
     1081 ttagggagtg ctttagtaat tgaccgctgt gtaaattgtg gggacactaa aagtatcatc
     1141 ggtgtgtctt ttaataaagg cggactcatt tgtaaaaagt gctacgatga gaagacaata
     1201 accttaaacg ctgaagaaat acaaatgtta cgctacggat ttatgattaa tgaagaagaa
     1261 ctcttgcgtc acgaatttag cgatgaagcc gcactttcac tcttaacctt gatgcttaca
     1321 tacttagaat ttacttataa ttataaaatt atgagtaaag atttaataaa ataatctaac
     1381 tacactcata gtgggttgac aaaaatgtat tttaataata tactttcctt taattgtgga
     1441 ggaattatgc ataaacattc atttgaaaaa atagtaaatc acttggtaac aagtggcttt
     1501 gtgttccaag gtagtgaaat ctatggtgga ctaagtaata catgggacta cggcccactt
     1561 ggaacatcgg ttaaaaataa cattagcaat ttatggtgga agaagtttgt tactgaatca
     1621 ccgtataacg tcgggattga cgcagcaata ttaatgaatc cgaaagtatg gcacgcgaca
     1681 ggtcacgtca caacctttaa cgacccgctg attgattgtc gaaactgtaa agctcgtcat
     1741 cgcgctgata acttaattga agatcaagtt aaaggactta atgttacggg cttaccaaac
     1801 gaagaaatga tgaaaatcat taaagagtat ggtgttaagt gtacagtatg tggtaaacaa
     1861 gattttactg atattcgtca atttaatatg atgtttaaaa cacatatcgg ggttactgaa
     1921 gatagtcaaa gtgaagttta tcttcggcct gaaacagcgc aagggatgtt tgttaacttt
     1981 aaaaatgtgc aacgtacgat gcgcaaaaaa ctcccgtttg gtatcgcggg tatt
//
LOCUS       DAOSEX010000159         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_55250_length_2033_cov_2.823054, whole genome shotgun sequence.
ACCESSION   DAOSEX010000159 DAOSEX010000000
VERSION     DAOSEX010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55250_length_2033_cov_2.823054"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK612_03360"
     CDS             complement(<1..312)
                     /locus_tag="PK612_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH95177.1"
                     /translation="MADKNITNSYTADSIQVLEGLDAVRKRPGMYIGSTNIVGFHQLV
                     WEVVNNSTDEAMSGYGDKIIVTIHKNGSITIADEGRGIPTGINKQTGQPAVHLVFSTL
                     HA"
     gene            417..1100
                     /gene="plsY"
                     /locus_tag="PK612_03365"
     CDS             417..1100
                     /gene="plsY"
                     /locus_tag="PK612_03365"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOH95178.1"
                     /translation="MHPFIKFLTFLGFGLFGYLIGSINNAVIISNLFFKRDVRNYGSK
                     NAGGTNTGRVFGRKAGITVMVLDILKSVFVYWAITLVFHFTNVGLYVDHAATLYFAMI
                     LVALGHCYPVYYKFKGGKAVSVVAGFILATNWVFTILGALIFFLILKLRKMVSYASIL
                     MAAIIVALTPILFIPVLGRISFYPLVVDNLYYYIPTLVGLCILLILKHRTNIERLQKG
                     TESKITWIK"
     gene            complement(1170..1382)
                     /locus_tag="PK612_03370"
     CDS             complement(1170..1382)
                     /locus_tag="PK612_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016340731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HOH95179.1"
                     /translation="MPIGAAIGLAVFALILGAVLGYLISRKVFEKQLKKNPPITEKQI
                     RTMLTMMGRKPSEKQVREIMRGMQQS"
     gene            complement(1382..1939)
                     /gene="efp"
                     /locus_tag="PK612_03375"
     CDS             complement(1382..1939)
                     /gene="efp"
                     /locus_tag="PK612_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003538298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOH95180.1"
                     /translation="MINVTELRPGNYFTEAGEIYNVLDILLNKTAMRKMIAKLKVKNV
                     RTGAITEQTYNSGYQIDRISISKHKMTYLYDSGDFLVFMNTETFEQVELSKESLTWEL
                     NFIVPNSEVEMAGYEGEIFGITLAPKVALKITECAPGVKGNTVQNATKDAIVETGLRV
                     SVPLFIEEGEVIMVKTTDGSYDGRA"
BASE COUNT          552 a          393 c          351 g          737 t
ORIGIN      
        1 agcgtgtaat gtggaaaata cgaggtgtac cgctggttgt ccggtttgtt tattaatgcc
       61 tgtggggatc ccgcgtcctt cgtcagcaat cgtaattgag ccgtttttat gaattgtaac
      121 aatgatctta tcaccataac cactcattgc ttcgtctgta ctattattga cgacttccca
      181 gacgagttgg tgaaaaccaa caatgtttgt tgacccgata tacatcccag gtcttttgcg
      241 taccgcatcg agtccttcta aaacttgaat cgaatcagcg gtatatgaat ttgtaatatt
      301 cttatctgcc atttgatttt tccttatcat ttcacaatac tttgttatta taacaaaaaa
      361 ttactttatt ttataattgg actattttaa tttatactat ttaagggagg ttacttatgc
      421 atccatttat aaagttctta acattcttag gattcggact ctttggctac ttaattggct
      481 cgattaataa cgctgtcatt attagtaatc tcttttttaa acgtgatgtc agaaattatg
      541 gcagtaagaa tgcgggcggg acaaatactg gtcgtgtctt cggccgtaaa gcaggaatca
      601 ctgtaatggt gcttgatatt ttaaaaagtg tttttgtata ttgggcgata accctagtct
      661 tccacttcac taatgtggga ctttatgttg atcacgcagc aacactttac tttgcaatga
      721 ttttagtcgc gcttggccac tgttatccag tgtattataa atttaaaggc ggtaaagctg
      781 tgagtgtcgt cgctggtttt attcttgcca caaactgggt atttacgatt ttaggggcgc
      841 ttattttctt tttaattctt aaattacgca agatggtcag ctatgcatcg atcttaatgg
      901 ccgctattat cgttgcacta acaccaattt tatttatccc agtacttggt cgtattagtt
      961 tttatccgct cgtcgtcgat aatctttatt attacatccc gacactagtc ggtttatgta
     1021 tcttattaat tttaaaacac cgcacaaata ttgaacggct ccaaaaaggc actgaatcaa
     1081 aaattacgtg gataaagtaa ttcttgccac taataaataa aagctaggac gtgtgtccta
     1141 gcgtttttat ttacatataa tcttaataat taagattgtt gcattccgcg cataatttcg
     1201 cggacttgtt tttcacttgg tttacggccc atcatcgtta acattgtccg aatttgtttt
     1261 tccgtaatcg gtggattttt ctttaattgt ttttcaaata ctttacgact aatgagataa
     1321 cctaataccg cacctagaat tagcgcaaag actgctaaac ctatcgctgc tccaattggc
     1381 attatgctct cccatcgtaa ctaccgtcag ttgttttgac cataattact tctccttctt
     1441 caataaataa tgggacactg actcttagtc ctgtttccac aatcgcgtcc tttgtcgcgt
     1501 tttgtacggt gttaccttta acacctggcg cacattcggt aattttaagg gcgactttag
     1561 gcgctaaagt tatgccaaaa atttcccctt cataaccggc catttctact tcactattgg
     1621 ggacaataaa gttaagttcc caagttaatg attctttgga taattcaact tgttcgaaag
     1681 tttcagtatt cataaacact aagaaatctc cactatcata aagataagtc attttgtgtt
     1741 tgctaatact aatccgatca atttgataac cgctgttgta tgtttgttcg gtaatcgcac
     1801 cagtgcggac atttttaact ttaagtttcg caatcatttt acgcattgct gtcttattaa
     1861 gtaagatgtc aagcacgtta taaatttcac cagcttcggt aaaataatta ccagggcgta
     1921 attctgtaac attaatcata atttaaccct cctctaatta attttaaata gtctagcgtt
     1981 tttcgcggtg taacgtttgt ttattgcaac gtgggcaaaa tttcattttt tca
//
LOCUS       DAOSEX010000160         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_55634_length_2024_cov_2.337227, whole genome shotgun sequence.
ACCESSION   DAOSEX010000160 DAOSEX010000000
VERSION     DAOSEX010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55634_length_2024_cov_2.337227"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PK612_03380"
     CDS             <1..816
                     /locus_tag="PK612_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH95181.1"
                     /translation="PLIYGEKGITNFTATKMIDLGPVSTITGGEAANSVIDKVVIRLL
                     KDEDFIKYLTDNKVEYTVKMLPKNMDVTLFGKSAHGSLPELGVNAGVLAFKHLGAFYK
                     LPFLTHLAEKFKNPNGKTMDAYVATSLLGATTYNIGLLNYENGKLSFVVNFRYPENVE
                     VETHLAKLAQTIDVELEIGRSSKHLLFDPKSEFIQTLLKAYRDETGDTQSKPLAIGGG
                     TYAKECPNTVAFGSAFPSRSGDIHSANEHIYLDDFYTQMAIYARAIHYLGKKV"
     gene            813..1433
                     /gene="trmB"
                     /locus_tag="PK612_03385"
     CDS             813..1433
                     /gene="trmB"
                     /locus_tag="PK612_03385"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOH95182.1"
                     /translation="MRVKFKPGAIDYLKSLAFHTHVDDFALSKIKDINKPLVLEIGVG
                     KGDYLLEMATNYPEHFFIGIELNASVLSVAAQKLADANLNNVLILTGDVKYLLPKLSA
                     LSLNYIILNHSDPWPKKRHEKRRLTYSTLVNEYYKLLEEGGTLIFKTDNDDFADYSYI
                     TLSSVPFKELIFIKDYKGDYDFDAKTEYEHKFLNKGVKIKMIIGKK"
     gene            1442..>2024
                     /locus_tag="PK612_03390"
     CDS             1442..>2024
                     /locus_tag="PK612_03390"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95183.1"
                     /translation="MLTKRELKLLEKVTQIDAIPGLEQELAKFVKGELEKYGFTIVTD
                     NLGSVFGVKKSKVTQAPRIMVAGHLDEVGFSVREITDKGFIRVDARGGINANTMLASR
                     VRLTLDDGTKLMGSVGATAPHYGGEGNPTIDKLDFDFGFISKEDAVNAGVRVGQMIVA
                     DGPFEVLNGGKRLLAKAFDNRYGVFMALEMARYF"
BASE COUNT          655 a          303 c          427 g          639 t
ORIGIN      
        1 ccgctaatat atggtgaaaa aggtattact aatttcactg cgacaaagat gattgattta
       61 ggtccagtaa gcacgattac tggtggtgaa gctgctaata gtgtgattga taaagtcgtc
      121 attcggcttt taaaagatga agattttatt aaatatttaa cggataataa ggttgagtat
      181 acagtaaaaa tgttaccgaa aaatatggat gtaacacttt ttggtaaaag tgcgcatggt
      241 agtttacctg aacttggtgt gaacgccggg gtacttgctt ttaaacattt aggtgctttc
      301 tataaacttc catttcttac ccatttagcg gaaaaattta aaaatccaaa cggtaaaact
      361 atggatgcat atgtcgcaac ctcactgctt ggtgctacga catataatat cggattacta
      421 aattatgaaa acgggaaatt aagttttgtt gttaatttcc gttatccgga aaatgtggaa
      481 gtggaaacgc atttagcaaa attagcgcaa acaatcgatg tcgaattaga aattggtaga
      541 agtagtaaac atttattatt tgatccaaag agtgaattta ttcaaacact cttaaaagca
      601 tatcgcgatg aaacaggcga cacgcaaagt aagccacttg ctatcggggg cggaacttat
      661 gctaaagaat gtcctaatac tgttgcattt ggtagtgctt ttcctagtag aagcggggat
      721 attcattccg ctaacgaaca tatttattta gatgattttt atacacagat ggcaatttac
      781 gctcgtgcta ttcattactt aggtaagaag gtatgagggt aaaatttaaa ccaggtgcta
      841 ttgattactt aaaatcatta gcgtttcaca ctcacgttga tgatttcgca ttgtcaaaaa
      901 ttaaagatat taataaaccg ttagtgctag aaattggtgt tggtaagggc gattatcttt
      961 tagaaatggc tactaactat cccgaacact tttttattgg tattgaatta aatgcaagcg
     1021 tccttagtgt tgccgcacaa aaacttgcgg acgcaaactt aaataatgtc ctaatcttaa
     1081 caggtgatgt taaatattta ttaccaaaat taagtgcact ttcacttaat tacattattt
     1141 taaatcatag tgatccgtgg ccaaaaaagc gccatgaaaa gcgccgttta acttactcga
     1201 cactcgttaa tgagtattat aagcttttag aagaaggtgg cacactaatt tttaaaacgg
     1261 ataatgatga ctttgcagat tactcatata tcactttaag tagtgttccc tttaaagagc
     1321 ttatttttat aaaagattat aagggtgatt atgattttga tgccaaaacg gaatacgaac
     1381 ataaattcct taataaaggt gttaaaatta aaatgattat tggaaagaaa taggtgttta
     1441 tatgttaaca aaacgtgaac tcaaattact tgaaaaagta acgcagatcg atgcaattcc
     1501 tggcttagaa caagaattag caaagtttgt caaaggcgaa ttagaaaaat acggttttac
     1561 aatcgttacg gataatctcg ggtcggtttt tggtgtaaag aaaagtaaag taactcaggc
     1621 accgcgcatt atggttgctg gtcatttaga tgaagttggt tttagtgtcc gtgaaattac
     1681 tgataaaggt tttattcgtg ttgatgctag gggcggtatt aacgctaaca cgatgttagc
     1741 gagtcgcgta cgtcttacgc ttgatgatgg cactaaatta atgggaagcg ttggtgcgac
     1801 cgcgccgcat tatggcggag aaggtaatcc aacgattgat aaacttgatt ttgacttcgg
     1861 ctttattagt aaggaagacg cagttaatgc tggtgttcgt gttggacaga tgattgttgc
     1921 agatggtcct tttgaagtat taaatggtgg caagcgctta ctcgctaaag cttttgataa
     1981 ccgctacggg gtatttatgg cgcttgaaat ggcacggtac tttg
//
LOCUS       DAOSEX010000161         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_56992_length_1992_cov_3.223542, whole genome shotgun sequence.
ACCESSION   DAOSEX010000161 DAOSEX010000000
VERSION     DAOSEX010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56992_length_1992_cov_3.223542"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PK612_03395"
     CDS             complement(<1..233)
                     /locus_tag="PK612_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95184.1"
                     /translation="MAKKLDAFEKWKASTGINSWPKVRELLTDLSKKGYTQRQICERL
                     KLSEAYFSRLKKKHPEIEQAFIDADDYVLDAAFT"
     gene            complement(365..793)
                     /locus_tag="PK612_03400"
     CDS             complement(365..793)
                     /locus_tag="PK612_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95185.1"
                     /translation="MNAREYLERIKTLETEIRYMKMRCEYYDEMSLSIPGPCLDREVL
                     SPNKNLQAPFVKWLNKKFDIEIEIKNKEEELKNLKAEALLKIEAMNSEDYKCILIERY
                     FNHRNWDDIATKIYVSRSTIKRWHEDALAEFEAILNIEPR"
     gene            complement(783..>1992)
                     /locus_tag="PK612_03405"
     CDS             complement(783..>1992)
                     /locus_tag="PK612_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004058628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH95186.1"
                     /translation="PLRVGLISWPDELAKWDHLQFLKSSVVIGSEAERLRALAEKADI
                     YIINRENLDWLVRKSGYTFDFDTVVIDELSSFKNAKSKRHKALMQVRPYIKRIVGLTG
                     TPASNGLMDLFAEFKCLDNGARLGRFITRYREEYFLPDKRNGMVVFSYKPQIGAEERI
                     YKKISDITISMKAIDHLKMPGLIEVEHAVDLADEEKELYKELQEEMIAEIEHKEITSA
                     NAAVLTGKLLQMANGAIYSDNGDVVEVHEQKLDALEDVIEGANGKPLLVAYWFKHDLE
                     RIKRRLNKLGVIYETLDKPEAIRKWNAKELEVGLVHPASAGHGLNLQSGGNTLEWFGL
                     TWSLELYQQTIARIYRQGQKEKTVVIIHLVTKGTMDERVLKALQRKDATQSRLVDALK
                     AELKGGGYEC"
BASE COUNT          521 a          474 c          331 g          666 t
ORIGIN      
        1 gtaaaagcag catctaaaac atagtcatca gcgtcaataa aagcctgctc tatctcaggg
       61 tgtttctttt ttaacctcga aaagtacgcc tcactcaact taaggcgttc acagatttga
      121 cgttgcgtat atcccttttt agataaatca gtcaataatt ctcgaacctt tggccatgaa
      181 ttgataccgg ttgaagcttt ccacttttca aaggcatcga gtttcttcgc catcgtcttt
      241 cctccgaatt gtgtataaat aaaagaaccc atgattcctc acaggctctc cttttagctt
      301 tcgccaattt tacaatacta caactcttga cagattacaa cagtccaaag cggttcattg
      361 cggttcatcg tggttcaata tttaatatcg cctcaaattc cgctagtgca tcctcgtgcc
      421 aacgctttat tgtgcttcta ctcacatata tttttgtagc aatgtcatcc caattccgat
      481 ggttgaaata tcgctcaata agaatacatt tatagtcttc actgttcatt gcttcaattt
      541 taaggagtgc ttctgctttg agatttttca gttcttcttc tttattcttg atttcaattt
      601 cgatatcaaa ctttttatta agccacttta caaacggtgc ttggagattc ttattaggac
      661 taagcacctc tctatcaaga catggtccag gaattgataa actcatctca tcataatact
      721 cgcaccgcat tttcatgtat cttatttccg tttctagcgt tttaattcgt tctaagtatt
      781 ctctagcatt catagccacc tcctttaagt tcagctttaa gcgcatcaac cagtcgtgat
      841 tgtgttgcgt cttttctttg tagcgccttt agcactcttt catccatcgt cccttttgta
      901 acaagatgaa taattaccac ggttttctct ttttgcccct ggcgataaat cctagcgata
      961 gtttgctgat ataactctaa actccacgtt agcccaaacc actcaagcgt gttcccacca
     1021 ctttgaaggt taagaccgtg accagcggaa gcaggatgca ctaaccctac ttctaactct
     1081 ttagcgttcc actttcgtat tgcttctggt ttatctagtg tttcgtatat aacgcctaat
     1141 ttattaagcc ttcgtttaat ccgctctaaa tcatgcttga accaataagc gacaagtagc
     1201 ggtttgccat tagccccttc gataacatct tccaaggcat caagtttttg ttcatgtacc
     1261 tcgacaacat caccgttatc ggaataaatt gctccatttg ccatttgtag taatttccca
     1321 gtcagcacag ccgcattcgc ggaagtgatt tccttatgtt cgatttcagc aatcatttct
     1381 tcttgtagtt ctttatacaa ctctttttcc tcatccgcta aatcgactgc gtgctcaact
     1441 tcaattaacc ctggcatttt taggtggtca attgctttca ttgatatcgt gatgtccgaa
     1501 atctttttat aaatgcgctc ttccgctccg atttgtggtt tataggagaa gacaaccata
     1561 ccgttacgct tatcagggag aaagtattct tcacgatagc gggtgataaa gcgcccaagt
     1621 cttgccccat tatcaaggca cttaaactcc gcgaataaat ccattaatcc gttagaagct
     1681 ggcgttcccg ttaagccaac aatgcgctta atataaggtc taacttgcat caacgctttg
     1741 tgccgttttg atttagcatt tttgaaactt gataattcat cgataacgac ggtgtcaaag
     1801 tcgaaggtat atccgctttt tcgtacaagc caatcaagat tctcccgatt gataatgtaa
     1861 atatcggctt tttccgctaa ggcacgtaag cgctccgctt cactcccaat aacaacgcta
     1921 gatttaagaa attgaagatg atcccattta gcaagttcat cgggccacga gataagcccg
     1981 actcgaagcg ga
//
LOCUS       DAOSEX010000162         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_57650_length_1977_cov_4.279396, whole genome shotgun sequence.
ACCESSION   DAOSEX010000162 DAOSEX010000000
VERSION     DAOSEX010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57650_length_1977_cov_4.279396"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..660)
                     /locus_tag="PK612_03410"
     CDS             complement(43..660)
                     /locus_tag="PK612_03410"
                     /inference="COORDINATES: protein motif:HMM:NF018751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HOH95187.1"
                     /translation="MLEKISNFVQLKNETIERLKPYVLLKVDKETYPDVVKKRAELNK
                     ELGKVDKERKDLNKFVNASYQEITDLYKEALEIVDSKLEAVENERRDEVLQEIMNAYA
                     SEKLPLSFEKVFDEKWLNKSVDWKSELRFKVNRIRDELAVAKKLDREAQYIESGNLAD
                     ILKEEVESEEVCYTTFKLKNPTDDQISKVHALLTVLGVEYETHSE"
     gene            complement(654..1202)
                     /locus_tag="PK612_03415"
     CDS             complement(654..1202)
                     /locus_tag="PK612_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95188.1"
                     /translation="MLKFDSEKHIYYMEIPSVTQIIRSVVLPNQYDGVPQHILDKAAE
                     WGTAIHDAIERDSSLMLDEEQTLLFNAYKKLIDEHHIKIIKKEQMVFYDGEIKYAGTY
                     DCLADILGATVLIDIKTTYKLDLEYLSYQLSFYAMAIPEKIDALYVLWLPKKGKAKFI
                     KIDRVPDEILKETLKRYKESIC"
     gene            complement(1186..1770)
                     /locus_tag="PK612_03420"
     CDS             complement(1186..1770)
                     /locus_tag="PK612_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HOH95189.1"
                     /translation="MTIYEKINILRKRIKDANLKKTGENKFAGFTYYELADFLPQTID
                     LENELGLISMFTLRDDENADLVVCDTTSDAQVVFTVRVREANMKGALEIQKAGAENTY
                     AKRYAYLNYLNLTEADQVDDKDQSKVIDEKKSVEKPNNRDIMLYEIEIAAPEHLKNQM
                     LSYFKVGTFDELTDAQLLQAHKRTIKELQNAKVR"
     gene            complement(1774..>1977)
                     /locus_tag="PK612_03425"
     CDS             complement(1774..>1977)
                     /locus_tag="PK612_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH95190.1"
                     /translation="KIKLLRTEKGLTQAELCDLTGLSSSTIVYLENDRKKAQVETLIR
                     LNNVLTNGDEKVLLELIELNERR"
BASE COUNT          518 a          400 c          307 g          752 t
ORIGIN      
        1 ctgactgcta ctttaccgcc cggcaagtaa ttaagataaa tatcattcgc tatgagtttc
       61 atattcaacc ccaagaaccg taagcaaagc atgaactttt gatatttggt catcggttgg
      121 atttttcaac ttgaatgtcg tatagcaaac ttcttcactt tccacttctt cttttaggat
      181 atccgcaaga tttccgcttt cgatatattg tgcttcccga tctaactttt tagcaacagc
      241 taattcatca cgaatgcgat taactttgaa tcttaattca cttttccaat caactgattt
      301 attgagccat ttttcatcga atactttttc gaacgataac ggaagttttt cagatgcata
      361 agcattcata atttcttgca acacctcatc tctccgttca ttctcgactg cttcaagttt
      421 actgtcgaca atttccaacg cctctttata aaggtcggta atttcttgat aactcgcatt
      481 tacgaactta tttaagtctt tacgctcttt atcaactttg cctaattctt tgtttaattc
      541 cgcccgtttt tttacaacat cgggataagt ttctttgtct acttttaata aaacatatgg
      601 ttttaatctt tcaatcgttt cattttttaa ctgaacgaag ttacttattt tttctaacat
      661 atactctcct tatacctttt taaggtttct tttaatattt catcaggaac acggtcaatt
      721 ttaatgaact tcgctttccc tttttttggt aaccataaaa cataaagtgc atcaattttt
      781 tcgggaattg ccatcgcata aaacgataat tggtaggata aatattcgag atccaattta
      841 taggtcgtct tgatgtcaat aagcactgtt gcccctaata tatcggcaag acaatcataa
      901 gttcctgcat atttaatttc gccatcgtag aacaccattt gctctttttt tatgatttta
      961 atatgatgct catcaatcaa ctttttatac gcattgaata gcaatgtttg ttcttcatca
     1021 agcattaaac tgctatctct ttcaatggca tcgtgaatag cagttcccca ctccgctgct
     1081 ttgtcgagaa tgtgttgtgg cacaccatca tattgattgg gcaacacaac cgagcgtatg
     1141 atttgtgtaa cgctcggtat ctccatgtaa taaatatgct tttcgctatc gaactttagc
     1201 attttgaagt tccttaattg tccgcttatg tgcttgtaaa agttgtgcgt cggttaattc
     1261 gtcaaaagtg ccaactttga aataacttaa catttgattt tttaggtgtt cgggtgcggc
     1321 tatttcgatt tcataaagca taatatctcg gttgtttggt ttttcaacgg acttcttttc
     1381 atcgataact ttactttggt ctttatcatc gacttgatct gcttcagtca agtttaagta
     1441 attaagataa gcatagcgtt tcgcataggt gttttccgcc cctgcctttt ggatttctaa
     1501 agcccctttc atattggctt cacgcacacg aacggtaaat accacttgag catcgcttgt
     1561 tgtgtcgcag actactaaat cggcattttc gtcatctcgt agagtaaaca tcgaaataag
     1621 cccgagctca ttttctaaat cgatagtttg cggtaaaaag tccgctaatt catagtaagt
     1681 aaatccagca aacttatttt cgcctgtttt tttcaagttg gcatccttga ttctttttct
     1741 aagaatattg attttttcgt aaattgtcat tttctatctc ctttcattta actcgataag
     1801 ttcgagtaat actttttcat cgccatttgt taacacattg tttaagcgta ttaatgtttc
     1861 cacttgtgct ttttttctat cgttttcgag ataaacgatc gtggaggacg ataaccccgt
     1921 taaatcgcaa agttccgcct gtgttaagcc tttttcagtg cggagtagtt taatttt
//
LOCUS       DAOSEX010000163         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_57708_length_1976_cov_2.999479, whole genome shotgun sequence.
ACCESSION   DAOSEX010000163 DAOSEX010000000
VERSION     DAOSEX010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57708_length_1976_cov_2.999479"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK612_03430"
     CDS             complement(<1..314)
                     /locus_tag="PK612_03430"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOH95191.1"
                     /translation="MLKLMIGVVIATVIVIFAFSVINSNMSANPQGQSSMHVIDDNFL
                     SITVTGQVVKPGTYVMKKDETIGDLIMAAGGPTSNADARSYIEDTVLIAGVSYYIAPI
                     ND"
     gene            complement(377..856)
                     /gene="greA"
                     /locus_tag="PK612_03435"
     CDS             complement(377..856)
                     /gene="greA"
                     /locus_tag="PK612_03435"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOH95192.1"
                     /translation="MTNKYTLTQEGVDALEEELSVLVNVERPLVLKEIEEARSFGDLS
                     ENADYDAARNKQANIESRIMEIEHILAHKEIVEYSKTSKTVAVGNYVEVHDRTNDDTF
                     SLRILGQFEGKPLNGTVSVDAPIAQAILGKKVGDRVNVKAQPPYEVEILNISTEPIE"
     gene            complement(936..1184)
                     /locus_tag="PK612_03440"
     CDS             complement(936..1184)
                     /locus_tag="PK612_03440"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOH95193.1"
                     /translation="MKENDVIVITTEEGGEEEFIILFTYENEERGTKYVFFYDENNDE
                     EVMCAKYFDDGTLEYVEDEEELAEMEEVFRAYESELEE"
     gene            complement(1171..1605)
                     /gene="ruvX"
                     /locus_tag="PK612_03445"
     CDS             complement(1171..1605)
                     /gene="ruvX"
                     /locus_tag="PK612_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOH95194.1"
                     /translation="MSKILGLDLGTKTLGIAISNVDATFAVAYENYVFPPYYYKKARA
                     HVLAICAKENISEIALGYPLNTDGTVGERAKSAERFRDDLLREGSPPIVLVNETYTTL
                     AATEELERLGYKKEQIKAQIDAVAAKYILETYLERKRSNERK"
     gene            complement(1598..>1976)
                     /locus_tag="PK612_03450"
     CDS             complement(1598..>1976)
                     /locus_tag="PK612_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH95195.1"
                     /translation="TMLKTAELHRTASALTPKKHGDTNIYLEYFTTSNREDLGVIADK
                     VRSTDECALLVLVGNKTNDFPLLISVSKELSKSLNANKVMRALTSAFGGRGGGRPDFA
                     NGVLLNLPEKTKALATILKVLDE"
BASE COUNT          554 a          426 c          324 g          672 t
ORIGIN      
        1 aagtcattaa tcggagctat gtaataacta acaccagcaa taagcactgt gtcttcaata
       61 taagagcgcg catcagcgtt acttgttggt ccgcccgctg ccataattaa gtcaccaatt
      121 gtttcgtctt ttttcatcac atatgttcct ggttttacga cttgtcccgt cactgttatt
      181 gataaaaagt tatcatcaat aacatgcatt gaactttgtc cttgcggatt agcgctcata
      241 ttgctattaa tcacactaaa ggcaaagatg acaataactg tagcgattac tacaccaatc
      301 attagcttta acataaataa aaaacctcct actaataagt aggaagttat ttatcttttt
      361 actacaaaaa tattaattat tcgattggtt cagtcgaaat atttaaaatt tccacttcat
      421 agggaggttg ggcttttacg tttacgcggt caccaacttt ttttcctaaa atggcttggg
      481 caatcggggc atcaacagaa actgtaccat taagtggttt accttcaaat tgtcctaaaa
      541 tacggagcga gaaagtatca tcatttgtgc ggtcatgaac ttcaacatag ttcccaacgg
      601 caactgtttt actcgttttg gaatattcaa cgatttcttt atgcgctaaa atgtgttcaa
      661 tttccataat ccggctttcg atattggctt gtttattacg ggcagcgtcg taatctgcgt
      721 tttcacttaa gtcaccaaaa ctacgggctt cttcgatttc tttgagcact agaggccgtt
      781 ctacatttac gagaacactt aattcttctt ctaaggcgtc aaccccttct tgggttaaag
      841 tatatttatt agtcatagtc atttctccta tataaattgc taatgcatta taacataatg
      901 cttataattc tcaaccaaaa acaaaaggga agcggttact cttctaattc gctttcgtag
      961 gcgcggaata cttcttccat ttccgcaagt tcttcttcgt cttcaacata ttctaatgtc
     1021 ccgtcatcaa aatacttcgc gcacatcact tcttcatcat tattttcatc atagaaaaag
     1081 acatattttg tgcctcgttc ttcattttca tacgtaaaaa gaataataaa ctcttcttct
     1141 ccgccttctt cggttgtaat aacaatcaca tcattttctt tcattgcttc ttttcctttc
     1201 taaataagtt tctaatatgt attttgccgc gacagcgtca atctgtgctt taatttgctc
     1261 ctttttataa ccgagtcgtt caagttcttc ggtagccgca agcgtcgtgt aagtttcatt
     1321 aactaataca ataggcggcg aaccttcacg caagagatca tcacggaatc tttccgcaga
     1381 ctttgctctt tccccaacag tgccatcagt gttaagcggg tagccaagcg caatttcact
     1441 aatgttttct ttagcgcata ttgctaagac atgggcacgc gcttttttat agtaatacgg
     1501 aggaaataca taattttcat aagcaactgc aaatgtcgcg tcaacattac ttatcgctat
     1561 cccaagcgtt ttagttccta agtctaagcc taatatttta ctcatcaagc acctttaata
     1621 ttgtcgctaa tgcttttgtt ttttctggta agtttaagag cacaccgtta gcaaagtcag
     1681 gtcggccgcc accgcgtcca ccaaaagcac ttgttaatgc gcgcattact ttattagcgt
     1741 ttagcgactt tgataactct ttactcacac taataagcag tgggaagtca tttgttttat
     1801 taccaactaa cacaagtaaa gcgcactcat cagtgcttct cactttatcg gcaataactc
     1861 ctaagtcttc acgatttgaa gtagtgaaat attctaaata tatattcgta tcaccgtgct
     1921 ttttcggtgt tagagcggaa gcagtacgat ggagttcagc tgttttaagc attgtg
//
LOCUS       DAOSEX010000164         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_58418_length_1960_cov_3.220472, whole genome shotgun sequence.
ACCESSION   DAOSEX010000164 DAOSEX010000000
VERSION     DAOSEX010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58418_length_1960_cov_3.220472"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PK612_03455"
     CDS             complement(<1..443)
                     /locus_tag="PK612_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOH95196.1"
                     /translation="METKIFKANEIHLLANIAKEGSIIAFPTETVFGLGVVYDNEEAF
                     NNLVKVKERKTDKPFTLMVGYTRDIENYAVLNTKTKNLIDVFMPGEITLIIEAKPNLP
                     KHVTLGSKYIGIRVSANYEVANLLNLIGKPLLVPSANKRDEKPALT"
     gene            complement(433..1263)
                     /gene="prmC"
                     /locus_tag="PK612_03460"
     CDS             complement(433..1263)
                     /gene="prmC"
                     /locus_tag="PK612_03460"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOH95197.1"
                     /translation="MLTLYSLKQNTLKLHNFLDKNDVKLALMHVYNIDSYTDYVLKLH
                     TPIEEKQEFYAIINRLVKGEPIQYILGKAYFYDRYFFVNKNVLIPRVETEELVDIVLK
                     DVKENTGNLLDLGTGSGAIALTLKAHSKLSVFASDISKKALEVAQKNDCENSVTFVHG
                     DLLAPFINKEIAFDYIVANLPYIKREEEVEQKVKDYEPESALYNSDPNIFERLFKEVK
                     MLNFPKTGLSIYLEIGTNQDDEVSNLARRYLGENVKIDVVNDMHGKKRFVIIKDIYGN
                     "
     gene            complement(1263..>1960)
                     /locus_tag="PK612_03465"
     CDS             complement(1263..>1960)
                     /locus_tag="PK612_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOH95198.1"
                     /translation="NIFAGDLFRMYVKYAERQGWQMQILDENSSAVGGFSQISFMIKG
                     KGVYSKLKYESGGHRVQRVPKTEASGRIHTSIATVLVMPEAEEVDVEIRNEDLEIDTF
                     RSQGAGGQNVNKVESAVRITHKPTGISVACQVEKSQIQNREIAMRLLRARVLAMKVAE
                     QEEKIGAERKLKVGTGERSEKIRTYNYPQNRVTDHRIGFSTLKLDLVLEGNLEEIIEA
                     LVHYDEQQKMVNS"
BASE COUNT          605 a          374 c          263 g          718 t
ORIGIN      
        1 gttaaagcag gtttttcatc ccgcttatta gcgctaggta caagtagtgg tttcccaatt
       61 aaatttaaaa gattagcaac ttcataatta gcgcttactc ttatgccaat atatttagaa
      121 cctagggtga cgtgtttcgg taaattaggt tttgcttcaa taattaaagt tatttctcca
      181 ggcataaata catcaattaa atttttagtt ttagtgttta aaaccgcata attttcaata
      241 tctcgtgtat aaccaaccat taaggtaaac ggtttatcag ttttacgttc tttaactttc
      301 actaaattgt taaaggcttc ttcattatca taaacaacac caagaccaaa tactgtctca
      361 gtcggaaaag caataatact cccttctttc gcaatgtttg ctaataaatg aatttcattt
      421 gctttaaata ttttagtttc cataaatatc cttaattatg acaaagcgct ttttaccgtg
      481 catatcattc acaacatcaa ttttaacatt ttctcctaag tagcggcgcg ctaaattact
      541 tacttcgtcg tcttgattag tgccaatctc taaataaatt gataatcctg ttttgggaaa
      601 gtttaacatt ttaacttctt taaatagtct ttcaaaaata tttggatcac tattataaag
      661 tgcgctttcg ggttcataat cttttacttt ttgttcaact tcctcttcgc gctttatata
      721 aggtaaattc gcaacaatat aatcaaaagc gatttcttta ttaataaatg gcgctaataa
      781 gtcgccatga acaaaagtta cactattttc acaatcgttt ttttgggcga cttctaatgc
      841 ttttttactt atatcactag cgaaaacact taatttggaa tgagctttta aagttaatgc
      901 aatcgcaccg cttcctgtac ctaaatcaag taaattacct gtattttctt ttacatcttt
      961 aagcacaata tccactaatt cttcagtttc aacacgcggt attaacacat ttttatttac
     1021 gaaaaaatac cgatcataaa aataagcttt acctaaaata tattgaatag gttcgccttt
     1081 aacaagacga ttaataattg cataaaactc ttgcttttct tcaatgggag tatgcaattt
     1141 taaaacataa tctgtatacg aatcaatatt ataaacatgc attaatgcta attttacatc
     1201 atttttgtcg agaaaattat gtagttttaa tgtattttgt tttaaagagt ataaagttaa
     1261 cattaactat ttaccatttt ttgttgttca tcataatgca cgagcgcttc aataatttct
     1321 tctaaatttc cttcaagcac taaatctaat tttaatgtgc taaagccaat acggtgatca
     1381 gtaactctat tttgcggata gttataagtt ctaatctttt cacttcgttc gccagtgccg
     1441 actttaagtt tacgttcagc accgattttt tcttcttgtt cggcaacttt catcgctaag
     1501 acacgggcgc gtaataaacg cattgctatt tcacgatttt gaatttggct tttctccact
     1561 tgacaagcaa cactgatacc agttggttta tgcgtaatac gtacagcgga ttcaactttg
     1621 ttaacatttt gtccaccagc gccttgactt ctaaaagtat caatttctaa atcttcatta
     1681 cgaatttcaa catcaacttc ttctgcttca ggcatcacta acacagtagc gattgaggta
     1741 tggattcttc cgcttgcttc agtttttggt acacgctgca cacgatgccc accactttca
     1801 tatttaagtt ttgaataaac acctttacct ttaatcataa aggagatttg cgaaaagcca
     1861 ccaacggcgc tactattttc atcaaggatt tgcatttgcc aaccttgtct ttcagcatat
     1921 ttaacataca ttctaaatag atcacccgca aaaatatttg
//
LOCUS       DAOSEX010000165         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_58472_length_1959_cov_2.778887, whole genome shotgun sequence.
ACCESSION   DAOSEX010000165 DAOSEX010000000
VERSION     DAOSEX010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58472_length_1959_cov_2.778887"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="PK612_03470"
     CDS             complement(<1..1615)
                     /locus_tag="PK612_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH95199.1"
                     /translation="MRKRKTLILFLLGILTLTGCGKNSSSEDAVSSSDVVSSSETSSE
                     ETSVSVSEAERYTITFYSEGGTSVNPITAEAGAAITKPDNPVRQGYTFVDWFEDKGTW
                     EVAFVFDFMPERDVVLYAKWQAQTLDEIEAYNNSLAASSKPNHLYIHYLRFNNTAEEY
                     NNWDIWVWPDRHTGRMFDFVKEDGVIKTDNYGGAMIEVDLGNIYTDGGHDGNGNKTDE
                     TVNFSPNEKLVDKIGFLITYKPSRSQGTHWTSDGGDKFLLTKDALEYALNGSLHIFAV
                     QENTRNFTYRYGGEVYDNPYEYDDGNSLSANYNDVDSSGTPDAVSATSPLMKNKGVGY
                     QIMVGSFADSDGDCFGDIRGITLSLDYLESLNVEAIWLTPVQISDSYHGYDTIDYYNI
                     DPKFGSKTSPHAIDGEITQESANADYLELLSAARERNIAVVMDLVINHTSINNLLFQE
                     SLSLNPEFRAYYHWRNFKENDNWYQYSNYNYYYYGKFASSMPELNFDYQKTRDKIVDI
                     MSHWVNAGVDGFRIDAVKHAYMKEEVATAA"
BASE COUNT          611 a          392 c          324 g          632 t
ORIGIN      
        1 ccgcagcggt cgcaacttct tctttcatat aagcatgttt aaccgcatca atccggaatc
       61 catcgacccc ggcattaacc cagtgactca taatatcaac aattttgtca cgtgtttttt
      121 gataatcaaa attaagttct ggcattgaac tagcaaactt accatagtaa taataattgt
      181 aatttgaata ttggtaccaa ttatcatttt ctttaaagtt ccgccagtga taataagcac
      241 ggaactctgg atttaagctt aaactttctt ggaagagtaa gttatttatt gaagtgtgat
      301 taatcactaa gtccataacg acggcaatat tacgttctct tgcagcgcta agtagttcta
      361 aataatcagc gtttgcgctc tcttgtgtaa tttcaccatc aatagcgtgc ggactagttt
      421 tactaccaaa ctttggatca atattgtaat aatcaattgt gtcatagccg tgataactat
      481 cactaatttg cactggtgtt aaccaaattg cttctacatt aagtgactct aagtaatcaa
      541 gacttaaagt gataccgcga atatccccaa agcaatctcc atcactatca gcgaaagagc
      601 cgaccataat ttggtaacca acgcctttat ttttcatgag cggactagtc gcagagacag
      661 catcaggtgt cccgctagaa tcaacatcat tataatttgc ggataaacta ttcccgtcat
      721 catattcata aggattatca taaacttcgc caccataacg ataagtaaag ttacgggtgt
      781 tttcttggac agcaaaaatg tgtaatgaac cgtttaatgc atattctaat gcgtctttag
      841 taagtaaaaa cttatcgcca ccgtcactcg tccagtgtgt accttgactg cgtgaaggtt
      901 tataggtaat taaaaaccca attttatcaa ctagtttttc atttggacta aaattaactg
      961 tttcatctgt cttattaccg tttccgtcat gtcccccatc agtgtaaatg ttcccgaggt
     1021 ccacctcaat catcgctcca ccgtaattat cagttttaat aacaccgtct tctttaacga
     1081 aatcaaacat tcttccagtg tggcgatcag gccataccca aatgtcccaa ttgttatatt
     1141 cttccgctgt attattaaat cttaaatagt gaatataaag atgatttggc ttacttgatg
     1201 ctgcaagact attattgtat gcttcaattt catcaagtgt ttgtgcttgc cacttagcgt
     1261 ataagacgac atcgcgttca ggcataaagt caaagacgaa cgctacttcc caagtacctt
     1321 tatcttcaaa ccaatcgaca aaagtataac cttggcgaac tggattatca ggtttagtaa
     1381 tagcagcacc cgcttcagcg gtaattggat ttacgcttgt gccaccttca ctataaaagg
     1441 taatcgtata acgttcagct tcactaacag aaacacttgt ctcttcactt gaagtttcgc
     1501 tactactaac tacatcactt gaagatactg cgtcttcact tgatgaattt ttaccacaac
     1561 ctgttagtgt taaaatacca agtaagaata gtatcaatgt ttttcttttt ctcataggtg
     1621 actccttttt gataatgatt gctattttaa aatagcactt attagtggta ttattttaac
     1681 aataataacc acgcttttca attgctataa atccaaagta tcgtgggtta aatatggctt
     1741 atgaactaaa attgatataa ttactatgaa aggaataaaa atatatgcag aaaataaaac
     1801 gtttattatt atttggcgcg cttttaagcc ttagtcttac tggttgcggc gactcttcaa
     1861 gccttgaatt accaactagc gagcctcaag gaactgaaag cgaagtcgta agtgaagata
     1921 atagtgaaga aagtagtgaa gtacttagtg ctgaaagtg
//
LOCUS       DAOSEX010000166         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_58691_length_1954_cov_2.320695, whole genome shotgun sequence.
ACCESSION   DAOSEX010000166 DAOSEX010000000
VERSION     DAOSEX010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58691_length_1954_cov_2.320695"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK612_03475"
     CDS             complement(<1..448)
                     /locus_tag="PK612_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95200.1"
                     /translation="MDNLKTLMGNAYHENLTIEEVDAFLTGKKLADLSSGNYVAKDKF
                     SKIEAELKELREATKDYEEVKKQVETYQAKEKETALKQALVEAGIKDEFIDYIAFKVE
                     KGEIANDDKLVNNVKNYIKDKPQFAKVAEADKPVIKKTIETSIGNGF"
     gene            complement(577..1767)
                     /locus_tag="PK612_03480"
     CDS             complement(577..1767)
                     /locus_tag="PK612_03480"
                     /inference="COORDINATES: protein motif:HMM:NF017919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95201.1"
                     /translation="MNSQRIDDLVDVLGDNFSKSENVFVVRNQLELFRYVNDPEQWKA
                     KQLANMTKYRTEVLKMAKDEAEKVARQVRKVYLLAYKEIDADNIEITKTEVKGTIPKS
                     YAKVIAKAEREAIGSIIQMANVALKTHTQAVRVVSALATPDKLYDVIKRQTLKGINKG
                     LKIVYKNGRQMNFKSYMEMNVRTTANQEITNAQIESGAKLNQVFYMCDSYGDSAPDHA
                     PYQGKMYYNADSVIDERTRRYISDNHLMSMQDAIAMYQLTTRPNCRHKFHLVPSDVAM
                     SKSEKQVSEQYGFDKGAYKGSNYEKMQQQRYLERGVRKYKEKTNQMEKMYNETKDERY
                     LAEVKKMKSKTREWQRKTKSFVDENKLKRDYDRESIKAITDDIGARYDIRKETKKARE
                     MQSD"
BASE COUNT          428 a          418 c          350 g          758 t
ORIGIN      
        1 caaagccatt accgattgaa gtttcaatgg tttttttgat aacgggtttg tcggcttccg
       61 cgacttttgc gaattgcggt ttgtctttaa tgtaattttt cacattattg actaatttgt
      121 cgtcgttagc aatttcgccc ttttcgactt tgaacgcaat gtaatcaata aactcatcct
      181 tgattcctgc ttccacgagt gcttgtttca atgctgtttc tttctctttg gcttggtagg
      241 tttcgacttg ttttttgact tcctcgtaat ctttggtggc ttcgcgtaat tcttttaact
      301 cggcttcgat tttagagaac ttatcttttg cgacataatt gccactcgat aaatcggcta
      361 actttttgcc cgtcaagaac gcgtcgactt cctcgattgt gaggttttca tggtatgcgt
      421 tgcccatgag tgttttaaga ttatccatcc cttttctcct ttgatttagt ttagatttcg
      481 gtttactccg acattaagaa gcccgttttt aatcgatgtc gggacatcta ctaatttcat
      541 aataatatac aacggcgaat aaataaatac taatttttaa tcgctttgca tttcgcgtgc
      601 tttcttggtt tctttgcgaa tatcgtatct cgctcctata tcatcagtta tcgccttaat
      661 actctctcgg tcataatcgc gttttaactt gttttcatca acgaacgatt tggtcttgcg
      721 ttgccattca cgcgttttgc ttttcatttt tttaacttcg gcaaggtatc gctcatcttt
      781 ggtttcgtta tacatctttt ccatttggtt ggttttctct ttatatttgc ggacgccacg
      841 ctctaaatat cgttgctgtt gcattttctc gtaattgcta cctttgtatg cgcctttatc
      901 aaagccatat tgctcgctga cttgcttttc gctcttagac atcgcgacat cgcttggcac
      961 taaatggaac ttgtgtcggc aattcggtcg tgtcgttaat tgatacatcg ctattgcgtc
     1021 ttgcattgac attaagtgat tatcgctgat gtatcttctt gtgcgttcat caatgacact
     1081 atcggcattg taatacatct tgccttgata tggggcgtga tctggtgctg aatcgccata
     1141 actatcgcac atatagaaca cttggtttaa ctttgcaccg ctttcaattt gagcgtttgt
     1201 gatttcttgg tttgcggtgg tgcggacatt catttccata tatgatttga agttcatttg
     1261 ccgaccattt ttataaacaa ttttaagtcc cttattaata ccttttagtg tttggcgttt
     1321 gatgacatca tataacttgt cgggtgttgc caatgcactg acaaccctta ctgcttgcgt
     1381 atgcgtttta agggcgacat ttgccatttg aataatgcta ccgattgcct cacgctcggc
     1441 tttggcgatg acctttgcgt atgattttgg tattgtgcct ttaacttcgg ttttggtgat
     1501 ttcgatattg tcagcatcaa tttctttata tgctaataaa tacactttgc gaacttgtcg
     1561 agcaaccttt tcggcttcat ctttcgccat tttcaaaact tcggtgcgat acttcgtcat
     1621 attcgctaat tgtttggctt tccattgttc gggatcattt acatatctaa acaattctaa
     1681 ttgatttcta acgacaaaaa cattctcact ttttgagaag ttatcgccta aaacatcgac
     1741 taaatcatct atgcgctgac tattcattca cagcacaccc ctttattagt tattgcttgg
     1801 attataaaaa cccgttgctt gtatatcttc ggcagttatc ccaccgcttt gactatcaat
     1861 cttttcttgc agttcagtaa tcaatgtttc cttgttcgat tcatcgatat aaacctgctc
     1921 gacaaattgc cgaacggtca ttgcaccttg catc
//
LOCUS       DAOSEX010000167         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_59148_length_1944_cov_3.022234, whole genome shotgun sequence.
ACCESSION   DAOSEX010000167 DAOSEX010000000
VERSION     DAOSEX010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59148_length_1944_cov_3.022234"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PK612_03485"
     CDS             complement(<1..819)
                     /locus_tag="PK612_03485"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOH95202.1"
                     /translation="MKNTLHILEVAKKVGLSPADVILYGEDKAKIKSPFADIKPQGKL
                     ILVTAITPTPFGEGKTTMSIALQDAMWRSGTKSLLCLREPSLGPVFGIKGGATGAGRA
                     KLYPSDDINLHFTGDLHALTSSINLIAAVIDNHIFQGNALNLDPKRITWKRSLDMNDR
                     GLRKVRINQGKGNGTPRCEEFQITVASELMAIMCLASDPDDFIRRVKDIIIGYNYDGE
                     IVRLAALQIEDAIMHLMKQALLPNLVQTLEGNPVLVHGGPFANIAHGCNSIIATK"
     gene            complement(816..1697)
                     /gene="miaA"
                     /locus_tag="PK612_03490"
     CDS             complement(816..1697)
                     /gene="miaA"
                     /locus_tag="PK612_03490"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOH95203.1"
                     /translation="MIFVIGGVTASGKSDLAFRFAKAINGIIINADAFAFYKELNIGT
                     AKPTKEELAMVPTFLFNNISLDERFSIYNYQKEVRVILDEYLAKKVPIVLVGGSGLYI
                     RAALYNYNLQPEKELKIVDDHKISNTSLHKELEALDKEAASKIHPNNRKRVLRALALI
                     KTHAQTKTALEEVTTSEPLYPYTMICLDKENEVLHRDIETRVKLMFAKGLRMEAVTLS
                     KKYPRNAQGMQAIGYKEIVQNPNATDEELMNLIALRTRQYAKRQRTFFRNQFTATWFT
                     DKEEAFNYLRGKYEELL"
     gene            complement(1694..>1944)
                     /locus_tag="PK612_03495"
     CDS             complement(1694..>1944)
                     /locus_tag="PK612_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HOH95204.1"
                     /translation="DIIDAVITDKRLNQELLRTNAIATMACKASIKANHRLSIMEMQH
                     LIKELLACDNPFSCPHGRPTIIKFTKYELEKMFKRTGT"
BASE COUNT          564 a          388 c          330 g          662 t
ORIGIN      
        1 ttttgtcgca atgattgaat tacagccatg ggcaatattt gcaaaaggtc cgccatggac
       61 aagaacggga ttgccttcta atgtttgcac caaatttggt aataatgctt gtttcattaa
      121 gtgcattatt gcatcttcta tttgtaaagc cgcaagacgg acaatttcgc cgtcataatt
      181 ataaccaata ataatatctt tcacacggcg aataaagtca tcaggatcac tagcaagaca
      241 cataattgcc attagttcac tagcgacagt aatttggaat tcttcgcagc gcggagtacc
      301 gttaccttta ccttgattaa ttctaacttt gcgtaatccg cgatcattca tatccaaaga
      361 gcgtttccat gtaatgcgtt tcgggtctaa atttagggca ttgccttgga aaatatgatt
      421 atcaattact gcagcaatta agttaatgct tgaagtaagt gcatggagat ccccagtaaa
      481 gtgaaggtta atatcgtcac ttggataaag cttagcgcgt cctgcaccag tagcaccgcc
      541 cttaatccca aacactggac caagtgaagg ctcccgtaaa cagagtaaac tcttcgtccc
      601 acttcgccac attgcgtctt ggagagcaat tgacattgtc gtttttcctt caccaaatgg
      661 tgttggtgta atcgcagtca ctaaaattaa tttaccttgc ggcttaatgt cagcaaacgg
      721 acttttaatc tttgctttat cttcaccata aagaattaca tctgctggtg aaagaccaac
      781 tttctttgca acttctaaaa tatgtaatgt gtttttcata ataactcctc atactttcct
      841 cttagataat taaacgcttc ttctttatca gtaaaccatg tagcggtaaa ttgattacgg
      901 aaaaaagttc gctgtctttt cgcatattgt ctcgtgcgta aggcaattaa attcattagt
      961 tcttcatcag tggcattagg attttgcaca atctctttat aaccaattgc ttgcattccc
     1021 tgtgcatttc gcgggtattt tttacttaaa gtcactgctt ccattcttaa tcctttggca
     1081 aacattaact taactcttgt ttcaatgtcg cgatgaagca cttcattttc tttatctaaa
     1141 caaatcatag tgtacggata aagtggctca cttgttgtta cttcttcaag tgcagttttc
     1201 gtttgggcat gagttttaat taaagcaagt gcccgcagta cacgtttacg attattagga
     1261 tgaatcttag atgccgcttc tttatctaag gcttctaatt ctttgtgcag tgaagtatta
     1321 cttatcttat ggtcatcaac aatttttaat tctttttccg gttgtaagtt atagttatat
     1381 aatgcggctc taatgtaaag accgctgcca ccaacgagca ctatcggaac ttttttagct
     1441 aaatactcat ctaatataac tcgcacttcc ttttgatagt tataaataga aaagcgttca
     1501 tctaatgaaa tattgttaaa taaaaaagtt ggaaccatcg ctaattcttc tttggttggt
     1561 ttagcagttc ctatatttaa ctctttataa aaagcaaacg catcagcgtt aataataatt
     1621 ccgtttattg ctttagcaaa gcggaaggct aaatcacttt tcccgcttgc ggtcacacca
     1681 ccaatgacaa aaatcatgtg cctgtcctct taaacatttt ttctaattcg tacttcgtaa
     1741 acttaataat tgtcggccga ccatgcggac aactaaacgg attatcacac gctagtaatt
     1801 cttttattaa atgttgcatt tccataattg aaaggcggtg attagcttta attgatgctt
     1861 tacaagccat tgttgcaata gcgttagtgc gtaaaagttc ttgatttaaa cgcttatcag
     1921 taataacagc atcaatgata tctt
//
LOCUS       DAOSEX010000168         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_59473_length_1937_cov_3.289586, whole genome shotgun sequence.
ACCESSION   DAOSEX010000168 DAOSEX010000000
VERSION     DAOSEX010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59473_length_1937_cov_3.289586"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PK612_03500"
     CDS             <1..386
                     /locus_tag="PK612_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOH95205.1"
                     /translation="IAVEMGAKTGLAAVVAGFMFLLSAFIYPIFYIFTEPAVTSMALI
                     SVGGLIFVGNLKDVNFDDRIIGFTAFFTIMLILLTYSISDGLGLGLIMYVIMMFVSKR
                     GKEVKIPMYVVAGVFVIYFILKALV"
     gene            568..915
                     /gene="rplS"
                     /locus_tag="PK612_03505"
     CDS             568..915
                     /gene="rplS"
                     /locus_tag="PK612_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOH95206.1"
                     /translation="MNKNLVNEINASQLRTDLPEFSSGDTVKVAVKIREGDKERIQFF
                     EGVVIAMQGAGVNHTFTVRKMSSGIGVERVFFTHSPVIDSVEVLRRGKVRRNKIYYIR
                     KLSGRKARIKEKK"
     gene            1011..1769
                     /gene="lepB"
                     /locus_tag="PK612_03510"
     CDS             1011..1769
                     /gene="lepB"
                     /locus_tag="PK612_03510"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH95207.1"
                     /translation="MYISLYNYYMVIEEVLINEEISEAEINRKNKPRLRKALSVTWTI
                     LLVTAIVVSLFVTYVDLTYTPVYVDGPSMQPTLNDFHHRYYEFGLMDERPKARNNIKR
                     GDIVVFRRNGINIIKRVIALPLETIQIIDNPTGDEVFITLPEATEKFKLDEPYLSTSN
                     RYTTATYLNDSSPKYENGLKEPLTLEEKTYYLMGDNRANSSDSRAIGPVHFDNISGKL
                     VVIQGYTENVEVSESGKVKFIGQHYYVLWKWRYY"
BASE COUNT          607 a          312 c          398 g          620 t
ORIGIN      
        1 ggattgctgt tgaaatgggt gctaagactg gtttagcggc ggttgtagcg ggatttatgt
       61 tcttacttag tgcttttatt tacccaatct tttatatttt taccgaacca gcggttacaa
      121 gtatggcact tatcagtgtt ggtggcttaa tctttgttgg taacttaaaa gatgtaaact
      181 ttgatgatcg cattattggt tttacagctt tctttacaat tatgcttatt ttacttactt
      241 actcgattag tgatggctta ggtttaggac ttataatgta tgtcattatg atgtttgtta
      301 gtaagcgtgg taaagaagtt aaaataccga tgtatgttgt agcgggagta tttgttatct
      361 actttatcct taaagcttta gtttaaacat aagtttaata aacgctaata gcccacttta
      421 cgaaagagtg ggctttttta aaattttagt aattactctt tacataagag tagtcttttg
      481 gtatattaat ggagcgcaaa gtcgctgtgg tccgctgcca aaaggtaaga acacgaaaat
      541 aatattgcag gaaggagcga gttattcatg aataaaaatt tagttaatga aattaacgct
      601 tctcaacttc gtaccgatct tcccgaattc agtagtggtg atactgtcaa ggtcgctgtt
      661 aaaattcgtg aaggtgacaa agaacggatt caattttttg aaggcgtggt cattgctatg
      721 caaggcgcag gcgtcaacca cacattcact gtccgcaaga tgtcaagtgg tattggtgtt
      781 gagcgtgttt tcttcactca ttcaccagtc attgacagtg tggaagttct ccgtcgcggg
      841 aaagttcgtc ggaacaaaat ttattacatt cgtaaattat ctggtcgtaa agcccgaatt
      901 aaagagaaga aataagttaa aatcatttat gagagaaagc cagaaaactg gctttttttc
      961 ttataaaacg agtatttatt tgtgataata agtaggaaaa tgtacatcaa atgtacattt
     1021 cgttatataa ttactatatg gtcatagaag aagtacttat taatgaagaa ataagtgaag
     1081 cagagataaa tcggaaaaat aaacccagac tacgtaaagc gcttagtgtg acgtggacta
     1141 ttttactcgt gaccgctatt gttgtttcgc tttttgtgac ttatgttgat ttaacttaca
     1201 ccccagttta tgttgatgga ccaagtatgc aaccaacact caatgacttt catcaccgct
     1261 attatgaatt tggcttaatg gacgaaagac caaaagcccg caacaatatt aagcgtggtg
     1321 atatcgttgt atttcggcgt aacggtatta acattattaa gcgtgtgatt gctttgccac
     1381 tagaaactat tcaaattatt gataacccga caggggacga agtttttatt acactgcctg
     1441 aagcaaccga gaaatttaaa ctcgatgagc cctacttatc aacaagtaat cgttatacaa
     1501 ccgcaacata tttaaacgat agttctccaa aatatgaaaa cggacttaaa gaaccgttaa
     1561 ctttagaaga gaaaacttat tacttaatgg gtgataaccg tgcaaattcc agtgattcac
     1621 gggcaattgg tcctgttcat tttgacaata tcagcggcaa attagtcgta attcaaggtt
     1681 atacggaaaa tgttgaagtt agtgaatcag gtaaagttaa atttattggt cagcattatt
     1741 atgtgctgtg gaaatggcgg tactactaat gagtaaacaa attggttggt tcccaggtca
     1801 catgcaaaaa gcgacacgca agcttattga aacgctaaag atggttgata ttatcgttga
     1861 attaggcgat gcacggatcc cccacagtag tcgcgctact tacttaacgg cgattattga
     1921 acgaaagcca aatcttt
//
LOCUS       DAOSEX010000169         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_60891_length_1907_cov_2.974622, whole genome shotgun sequence.
ACCESSION   DAOSEX010000169 DAOSEX010000000
VERSION     DAOSEX010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60891_length_1907_cov_2.974622"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PK612_03515"
     CDS             complement(<1..227)
                     /locus_tag="PK612_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOH95208.1"
                     /translation="MTKIGIIISSLRENSNSQKIANNLLTLLEGKADAKVIDIKALPL
                     YNQDLDKEGETPESYTTFRNEVKSVNKLIVI"
     gene            complement(401..868)
                     /locus_tag="PK612_03520"
     CDS             complement(401..868)
                     /locus_tag="PK612_03520"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH95209.1"
                     /translation="MIEIRELKDNEISDFWQEHIKYLIEDGLIKEQSEIDYYSGLNYH
                     NKFEGYLKEATDKLHMVWFIKDGVKIGATQYKINKSGDGECYIYDFWVFPSYRGHNTG
                     HECFAALENYTKIDGAFYYKLDAEKENSIRFWKSLGFEECGMTPEGDILFVRR"
     gene            complement(1016..1705)
                     /gene="araD"
                     /locus_tag="PK612_03525"
     CDS             complement(1016..1705)
                     /gene="araD"
                     /locus_tag="PK612_03525"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HOH95210.1"
                     /translation="MYKTLKETTYNANIAIKKSGLVLLTWGNVSVKDDVLKVIAIKPS
                     GVAYEKMKADDIVVVDFDGNIIEGTLRPSVDLPSHLALYKAHQEIGAIVHTHSTFATA
                     WAQKGRAIPMYGTTHADYFSDDIPCAPYLTPEEMKDYEKNTGEVIAALIKKGRANETP
                     AAIIKGHGAFAWGKNAKDAVLHAIVLEEVAKIAYLTEAIPGQNNPLPEHIKKTHYERK
                     HGPNARYGQKK"
     gene            complement(1709..>1907)
                     /locus_tag="PK612_03530"
     CDS             complement(1709..>1907)
                     /locus_tag="PK612_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOH95211.1"
                     /translation="EKSAACWLVAGGAHHTTLVYDLSLEFLRDFANLLDIEFVHIGKD
                     ADPYILEEMMKNNDIRYRLRG"
BASE COUNT          549 a          385 c          279 g          694 t
ORIGIN      
        1 ctaataacta ttaatttatt aacactcttt acttcgtttc taaaagttgt ataactttca
       61 ggtgtttcgc cttctttatc taaatcttga ttatataaag gaagtgcttt aatatcgata
      121 actttcgcgt cagctttccc ttctaataat gttagtaaat tgttcgcaat cttttgtgaa
      181 ttactatttt cacgtaatga acttataata atgccaatct ttgtcataat tttcctcctt
      241 gtgcttcttt attataaagg caagtttaac ttttaattaa tcatttgctt aacacttaaa
      301 aaaacacgct taatatagtt tatttaccaa ttattaaaaa agatttatcc cccactttat
      361 ataccactta tcccccacta ttaatggtta ataagcacta ttaacgtcta acaaaaagga
      421 tgtcgccttc tggtgtcatt ccgcattctt caaagccaag tgacttccaa aaacggattg
      481 aattttcttt ttcagcatct agtttgtagt aaaacgctcc gtcaatcttt gtgtaatttt
      541 caagggcggc aaagcattcg tgtcccgtat tatgaccgcg gtaagatggg aacacccaaa
      601 agtcataaat ataacattcc ccgtcaccac ttttatttat cttatactga gttgcgccga
      661 ttttaacccc atctttaata aaccatacca tatgaagttt atcggttgct tcttttaagt
      721 aaccttcaaa tttattatga taatttaatc ctgaataata gtcgatttca ctttgttctt
      781 taattaatcc gtcttcaatt aagtatttaa tatgttcctg ccaaaagtca ctaatctcgt
      841 tgtcttttaa ttctctaatt tcaatcatgc aaaaatacgc tattttctcc tattttcgat
      901 aaaaataatt atactcttac taaataaaag tgtatatttt ttactcaaaa aaaacaccct
      961 tcttaatact tttttacaaa gggtgccttt ttataattat ttaaaagttt agtgcttatt
     1021 tcttttgacc ataacgagcg ttaggaccat gttttcgctc ataatgggtt ttcttaatat
     1081 gctctggtag cggattattt tgacccggga ttgcttctgt aagataggca atcttcgcta
     1141 cttcttctaa cacgatagcg tgcagcacag catctttagc atttttaccc cacgcaaaag
     1201 caccatgtcc tttgatgatt gccgctggtg tttcattagc ccgtcctttt ttaatgagcg
     1261 ccgcaattac ttcaccagta tttttttcgt agtctttcat ctcttctggt gttaaataag
     1321 gagcgcacgg aatatcatcg ctaaaataat cagcgtgtgt cgtaccatac attggtattg
     1381 ctctaccttt ttgtgcccac gctgtcgcaa atgttgagtg agtatggaca attgcaccaa
     1441 tttcttgatg tgctttatat aaggctaggt gtgaaggcaa atcaacagac ggtcgcaaag
     1501 ttccttcaat gatattcccg tcaaagtcga ctacaacaat atcatctgct ttcatctttt
     1561 catacgcaac accagacggt ttaattgcta ttactttaag tacatcatct ttaacagaca
     1621 cattgcccca tgttaaaagt actaaacccg atttcttaat ggcaatgtta gcgttatatg
     1681 tcgtttcttt taaagttttg tacatatttt accctcgtaa acgatagcgg atatcgttat
     1741 ttttcatcat ttcttcaagt atgtaaggat cagcgtcttt cccaatatgc acaaattcaa
     1801 tgtcaaggag attagcaaag tcacggagaa attcaagtga taggtcatac actagtgtgg
     1861 tgtgatgtgc cccgcccgct actaaccaac aagctgctga cttttct
//
LOCUS       DAOSEX010000170         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_61258_length_1900_cov_2.811924, whole genome shotgun sequence.
ACCESSION   DAOSEX010000170 DAOSEX010000000
VERSION     DAOSEX010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61258_length_1900_cov_2.811924"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PK612_03535"
     CDS             complement(<1..998)
                     /locus_tag="PK612_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOH95212.1"
                     /translation="MFTTTKSFIHEFKKRLTEKYGVGLDDSHITERYDILGSMVRDYA
                     NINWRKSRDAVNKKGKKQLIYFSMEFLIGRLLTNNLINLGIYNVVNKGLKQIGMNLHD
                     LEEQESDAGLGNGGLGRLAACFLDSIASLGYAGNGHCIRYEYGFFRQKIVDGKQVELP
                     DQWLVNGNVWEVRKPKHAVNVKFYGHAETYQKRNGEFGIITVNPTIVKAVPYDMSVVG
                     YRNDVANTLRLWSAEPSDEQLPRDESFDDYLRTLREINHGLYPDDSTEKGKMLRLRQQ
                     YFLVSAGLQSAIRGHFRRHKTLRNFHEHYVFQLNDTHPILAIPELMRILLDDYEYG"
     gene            complement(1035..>1900)
                     /locus_tag="PK612_03540"
     CDS             complement(1035..>1900)
                     /locus_tag="PK612_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOH95213.1"
                     /translation="YFFSENFMLPLSHDEVVHGKGTIINKMFGNYDQKFALTRNLYTY
                     QFAHPGKKLTFMGNEIASFDEWNESKSLPWNLKSYPKHDSVSRLVRDLNLIYRSEKAM
                     HVGEFDPRNFRWLMVDNRDQSVFAFQRKYGEETLIFVFNMTGNYYEYYDIGVEEEGTY
                     TEIFNSDKDVYGGYNQYNGLPVTTHAGPGPEMRPYRLTLKLGSFAAMILKKDLPAKKE
                     EPKVEEVKAKKPAAKKAPAKKAATTKKAEPKATKTATKSTATKKATKSATAKKTTTKK
                     ATTAKTKKDTK"
BASE COUNT          518 a          384 c          338 g          660 t
ORIGIN      
        1 cagccatatt cataatcatc aagtaaaata cgcattaatt ccggaattgc taaaatgggg
       61 tgcgtatcat taagttggaa cacatagtgt tcatggaaat tacgtaatgt tttatgacga
      121 cggaaatgac cgcgaatcgc actttgcaag ccagcactga ctaagaaata ttgctgtctt
      181 aagcggagca ttttaccttt ttcggtagaa tcatcaggat ataacccgtg attaatttcg
      241 cggagtgtcc gtaaataatc atcaaatgat tcgtcacgtg gtaattgttc atcgcttggt
      301 tcagcgctcc ataagcggag agtatttgcg acgtcattac gatagccgac aacactcatg
      361 tcatagggca ctgctttaac gatagtggga ttaacagtta taatcccaaa ttcaccattg
      421 cgtttttgat aagtttcagc gtgaccgtaa aacttaacat tcaccgcgtg ctttggtttc
      481 cgtacttccc agacgttgcc attgactaac cattgatcgg gtaattcgac ttgtttcccg
      541 tcgacaatct tttgccggaa gaacccgtat tcataacgaa tacagtgacc gtttccagcg
      601 taaccaagac tcgcaattga atcaaggaaa catgcggcaa ggcgacctaa accaccatta
      661 ccaaggccag catcactttc ttgttcttct aaatcatgaa gattcatccc aatttgtttt
      721 aggcctttgt taacgacatt ataaattcct aagttgatta ggttgtttgt cagtaaccga
      781 ccaattaaaa attccattga gaaataaatt aattgtttct tacctttctt attaacagcg
      841 tcacggcttt tgcgccagtt gatgttcgcg tagtctctta ccattgaacc taaaatgtca
      901 taacgttcag tgatgtgact atcatcaagg ccaacgccgt atttctctgt taatcgcttt
      961 ttaaattcat gaataaaact ttttgttgtt gtaaacataa attattcctt cctttattta
     1021 tttaactcac taaattattt agtgtctttt tttgttttag cagtagttgc tttttttgtt
     1081 gtagtctttt tggctgtagc actttttgtt gcttttttag tagcggtgct ctttgtggca
     1141 gtttttgttg ccttaggttc agcctttttt gttgtcgccg cttttttagc aggcgctttt
     1201 ttcgctgctg gtttctttgc tttaacttct tcgacctttg gttcttcttt ctttgcgggt
     1261 aaatctttct ttaaaatcat cgcggcaaat gaaccaagtt taagtgttaa gcggtatgga
     1321 cgcatttctg gtcctggacc agcgtgtgtt gtcactggta aaccgttata ttggttataa
     1381 ccaccataga catctttatc actattaaag atttcagtgt aagtaccttc ttcttcaaca
     1441 ccgatatcgt aatattcgta ataatttcca gtcatattaa agacaaagat taatgtttct
     1501 tcaccgtatt tacgttggaa cgcaaacaca ctttgatcac gattatcgac cattaaccat
     1561 ctaaagttac gagggtcaaa ttcaccaaca tgcatcgctt tttcactacg ataaattaag
     1621 tttaagtcgc gcactaagcg actaactgaa tcgtgttttg gatatgactt taagttccac
     1681 ggtaaactct tactttcatt ccactcatcg aagctagcaa tttcgttacc cataaaggta
     1741 agttttttac caggatgcgc gaattgataa gtatataagt tacgcgttaa ggcaaacttt
     1801 tggtcatagt taccaaacat cttattaata attgtccctt taccgtgcac tacttcgtcg
     1861 tgacttagtg gtaacataaa gttttcacta aagaaataat
//
LOCUS       DAOSEX010000171         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_61296_length_1899_cov_3.636659, whole genome shotgun sequence.
ACCESSION   DAOSEX010000171 DAOSEX010000000
VERSION     DAOSEX010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61296_length_1899_cov_3.636659"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="tilS"
                     /locus_tag="PK612_03545"
     CDS             <1..990
                     /gene="tilS"
                     /locus_tag="PK612_03545"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOH95214.1"
                     /translation="NFQNEARKVRYEFFEEIIKEHDLDALLTAHHADDVIETYLMQQN
                     SKRETFYYGIREEIVLNNSLVIRPLLNYYKNDLVFYCVEHKVPFAVDSSNLELTYTRN
                     KIRHNLVKKMSVEQKDKLLLDIKGRNKDLKAMTNKLELLLENSTISITEFIKLNNREQ
                     QHLLYMLFHNKGIADKFSKGRNNQIKDVVLSDKNSAMIKVIDSYYLVKYEDKFSLINI
                     NNYQPYKYIVETPETIKTPQFVAFFGKNNVFPNIPASEYPLTIATQGKNQKYKIKGYE
                     KTINRLFIDMKMPRHYRLIWPVVTNKNGEVIYVPRYRANYISKATDLFKIYIK"
     gene            1093..>1899
                     /locus_tag="PK612_03550"
     CDS             1093..>1899
                     /locus_tag="PK612_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH95215.1"
                     /translation="MSNPNPRPNPRSVPPRRSLRTLLPYLLLFAVIGVVIWLAFFTGN
                     AKTQALLQTELAAYLQTNAGVIDTITIVTTETTTKITGILKDGSKFTALVDRTFYENI
                     LQPTLFAADGTALVDFKMVIVNAYQRTFWNTIGPSLLYLVLFVVAMIFLYSRMAGAVS
                     SSNTKAMEFNRSRARLETKVKKTFDDVAGADAEKQEMMEIVDYLRNPKKYTKFGAKLP
                     KGVLLVGPPGTGKTLLAKAVAGEANVPFYSISGSDFVEMFVGVGASRVRDL"
BASE COUNT          672 a          317 c          324 g          586 t
ORIGIN      
        1 aacttccaaa acgaagcgag aaaagttcgt tatgagtttt ttgaagagat tattaaagaa
       61 cacgatcttg atgcactatt aacagcgcat cacgctgatg acgtgattga aacatattta
      121 atgcagcaaa atagtaaacg tgaaacattt tactatggaa ttagagaaga aattgttcta
      181 aacaactctc ttgttattcg tccgttacta aactattaca aaaatgatct agtgttttat
      241 tgtgtcgaac acaaagttcc ttttgcagta gacagttcaa atttagagtt aacatacacc
      301 agaaataaaa ttcgccacaa cttagttaaa aaaatgtctg ttgaacaaaa agacaaacta
      361 ctcctagaca ttaaagggcg caataaagat ttaaaagcaa tgacaaataa acttgaatta
      421 ttgttagaaa atagtactat ttccataacg gaatttatta aactaaataa tagggagcaa
      481 cagcatcttt tatatatgct ttttcataat aaaggaattg cagataagtt tagtaagggc
      541 agaaataacc agattaaaga tgttgtgtta agtgataaaa attcagcaat gattaaagta
      601 attgattcat attatttagt gaagtatgaa gataaatttt cattaattaa tatcaataat
      661 tatcagcctt acaaatatat tgttgaaaca ccagaaacaa ttaaaacccc gcaattcgtg
      721 gctttttttg gcaaaaacaa tgtatttcct aatattcctg ctagcgaata tcctttgaca
      781 attgctacac aaggaaaaaa tcaaaagtat aaaatcaaag gttatgaaaa gacaataaat
      841 cgtttattta ttgatatgaa gatgccgcgt cactatcgac ttatttggcc ggttgttaca
      901 aataaaaatg gtgaagtaat ttatgtcccg cgctatcgcg ctaactacat ttcaaaagca
      961 acggatttat ttaaaattta tattaaataa aacgcaaatg ttgaaatcta agattatact
     1021 ctgtataata actttcagtg tttatttatg ttacacatat tcgaaattaa tctataaccc
     1081 aaggaggtct taatgagcaa tccaaatcca cgtccaaacc ctagaagcgt tccaccgcgt
     1141 cgctcgctgc gaaccctttt accttattta cttttatttg ctgtaatcgg tgttgttatc
     1201 tggttagcat tttttacggg aaacgctaaa actcaagcac tacttcaaac cgaactagca
     1261 gcatacttac aaacaaatgc tggtgttatt gacacaatta ctattgtaac taccgaaacc
     1321 accacgaaaa ttactggtat tttaaaagac ggtagtaagt ttaccgcttt agtagaccga
     1381 acattttatg aaaacatctt acaaccaaca ttattcgccg ctgatggaac ggcacttgtt
     1441 gactttaaga tggttattgt taatgcttat caacgcacat tttggaacac tattggacca
     1501 agcttacttt atcttgtcct atttgtagtg gcaatgatat tcctttactc aagaatggct
     1561 ggtgcagtta gcagttcaaa tactaaagcc atggaattta atcgttcacg cgctcgctta
     1621 gaaacaaaag ttaaaaagac gtttgacgat gttgcaggcg ctgatgctga aaaacaagag
     1681 atgatggaaa ttgttgatta tttacgtaat cccaaaaaat atacaaaatt cggtgcaaaa
     1741 ctaccaaaag gtgtacttct tgtggggccg ccaggcaccg gtaaaacact tttagctaaa
     1801 gcagtagcag gtgaggctaa tgtgcctttc tactcaattt caggttctga ctttgttgag
     1861 atgtttgtag gagttggtgc ttcaagggtg agagatctt
//
LOCUS       DAOSEX010000172         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_61366_length_1898_cov_2.083559, whole genome shotgun sequence.
ACCESSION   DAOSEX010000172 DAOSEX010000000
VERSION     DAOSEX010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61366_length_1898_cov_2.083559"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..519
                     /locus_tag="PK612_03555"
     CDS             136..519
                     /locus_tag="PK612_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOH95216.1"
                     /translation="MKNVYTNMCMVYSGDKILVLNRVKKDWPGLTFPGGHVEDGELFS
                     ESVIREMKEETGLTIRNPEYVGQIVWPLPEQGINDVALLYRTNEFAGELLESVEGHVF
                     FIKKSELGNYQHSLDVDKVLELMFK"
     gene            complement(525..1400)
                     /locus_tag="PK612_03560"
     CDS             complement(525..1400)
                     /locus_tag="PK612_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95217.1"
                     /translation="MAIMSQLFNSYRFFEGIADMLGGIDFISYIFGFLSQFPIVVDII
                     IASAIIAALLRGIFKRFWKVVWRGVIFVILLIVLFATAEKLAPYIGSIPIPLRGTVNG
                     NAVEYANLGQIIYGVSIEAGNSETYAFEFTKLALKNLVVFFGMPALSLITPAISAITY
                     PLFSLLLPRKLKDAKLILPRIAISLGLTFIALVIFMAPAANFVPAMSAVKETMSEGTL
                     LYKFLNPEVINFLELFTPQKSVMLKIVDIGNISGKIKIFEEFTVDGVVIQLKDAVEAL
                     LEALKTIPYEAPAAG"
     gene            complement(1507..>1898)
                     /gene="eno"
                     /locus_tag="PK612_03565"
     CDS             complement(1507..>1898)
                     /gene="eno"
                     /locus_tag="PK612_03565"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513248.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOH95218.1"
                     /translation="TDRLSHKIQIVGDDLFVTNPAILKEGIEKGIANSILIKLNQIGT
                     LTETLDAMQMAERAGYTCVVSHRSGESEDTTIADLAVALNAGQIKTGSMSRTDRIAKY
                     NQLIRIEDDLGERAIYPGLSAFYNLRK"
BASE COUNT          633 a          328 c          348 g          589 t
ORIGIN      
        1 ccgacgtatc ccaatccaac aactgtaatt ttattaatat gcatatgctt cccttaaatt
       61 ataacattta tcaaattatt cactaatgca gagtaccttt tttctaaaac ttttgattta
      121 tggtacgatt ttaatatgaa aaatgtttac acaaatatgt gcatggttta ttcaggtgat
      181 aagattttag tgcttaaccg tgttaaaaaa gattggccgg gccttacttt tccaggtggc
      241 catgttgaag atggtgaatt atttagtgaa agcgtaattc gtgaaatgaa agaagaaaca
      301 ggtttaacaa ttcgtaatcc ggaatacgtt ggtcaaatcg tctggccgct tcctgagcaa
      361 gggataaatg atgttgccct tttatatcgc acaaatgaat ttgctggtga acttttagaa
      421 agtgttgaag gtcacgtctt tttcattaaa aaaagtgagc ttggtaatta ccaacactca
      481 cttgatgttg acaaagttct agaactaatg tttaaataat aagattatcc tgctgctgga
      541 gcttcgtaag gtatagtttt aagtgcttca agtaaggctt caacggcatc ttttagttga
      601 ataacaaccc catcaacagt aaattcttca aagattttga tcttgccaga aatatttcca
      661 atatcaacaa tttttaacat tactgatttt tgtggtgtaa ataattctaa gaaattaata
      721 acttcgggat ttaagaattt gtagagcaat gtgccttcac tcattgtttc tttgacagca
      781 ctcatcgctg gtacaaagtt tgcggcggga gccataaaga taacaagggc gataaaggtt
      841 aagccaaggc taattgcaat acgcggaagg atgagtttag cgtcttttaa cttacgtgga
      901 agtaataaac taaataacgg ataggtaatt gctgaaattg ccggtgttat gagtgataaa
      961 gcgggcatcc caaagaaaac aacaagattt ttaagcgcta gttttgtaaa ttcaaaagca
     1021 taggtttcac tattacctgc ttcaattgaa acaccataaa ttatttgtcc taagtttgca
     1081 tactcaacag cgttgccatt tactgtccca cggagcggga taggaatact accaatataa
     1141 ggtgcaagtt tttctgctgt tgcaaataag acgattaaaa gaataacaaa gatgacacca
     1201 cgccagacga ctttccaaaa tcttttgaaa ataccgcgta gcagcgcagc gataatcgca
     1261 cttgcaatga tgatatcaac gacaattggg aactgggata aaaaaccaaa aatatatgaa
     1321 ataaaatcta ttccaccaag catatcggca ataccttcaa aaaagcgata agaattaaag
     1381 agttgactca ttattgccat aagattacct cctgcttaaa ttatacataa ttaataatta
     1441 aaaaaatata gatttataaa aaaagagggt ctcccctctt ttaatgtgac aagtaatatt
     1501 acgcaattat ttgcgtaaat tatagaaagc acttaaacca ggatagattg ctctttcacc
     1561 aagatcgtct tcaatgcgaa ttaattggtt gtatttagca atccggtcag tacggctcat
     1621 cgaaccagtt ttgatttgac cagcgtttaa agcgactgct aagtcggcaa tagttgtatc
     1681 ttcactttca ccagaacggt ggctaacaac acaagtgtac ccagcgcgtt ccgccatttg
     1741 catagcgtct agtgtttcag ttaaggtacc aatttgattt aatttaataa gaatggagtt
     1801 tgcgatgcct ttttcaatac cttctttaag aattgctggg tttgtaacga ataagtcgtc
     1861 accaacaatt tgaatcttat ggcttaaacg gtcagtta
//
LOCUS       DAOSEX010000173         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_61401_length_1897_cov_2.383822, whole genome shotgun sequence.
ACCESSION   DAOSEX010000173 DAOSEX010000000
VERSION     DAOSEX010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61401_length_1897_cov_2.383822"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="PK612_03570"
     CDS             <1..1090
                     /locus_tag="PK612_03570"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="damage repair protein"
                     /protein_id="HOH95219.1"
                     /translation="GLSNVMRKFELPRGIPYIFAKPRMELYVQKSTEFNALLLNYFAK
                     EDWYPYSIDETFIHLTPYLELYKKTPEELIEFIIKEIYDHLKLYVTVGIAPNMFLAKC
                     VLDLEAKKDPRYYARWTMEDVKTKLWQIKPLHKIWGIGKRYERRLHKLGLFTMGDIAK
                     CPKAKLKEEFGIMGEELWEHANGIDNTDLSEKYVPLDKSLTSGQVLFRDYEVNEAPVL
                     IREMTDELCMRMRKNNLKAKKVGLSIMTSKPESNTYSKSMSLLLPTCSNTELFKAFME
                     VFNKFPLEGAKIRSLHLSVSGLSSDPYEQLSLIEDYVTQNTKEQRMWETLDELHARYG
                     KDKIMRANALTRASTYLRRTKQIGGHNK"
     gene            1099..1392
                     /locus_tag="PK612_03575"
     CDS             1099..1392
                     /locus_tag="PK612_03575"
                     /inference="COORDINATES: protein motif:HMM:NF020438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HOH95220.1"
                     /translation="MKKFLPFASLKEQALFLAEMQQQKKRVEKPTLSEDQCAEINRVL
                     SEYTGETVLIYYYNRGFIKTHQAKIKKIDTLYKRLVFTDLTVSFTNLLHIEII"
     gene            complement(1393..>1897)
                     /locus_tag="PK612_03580"
     CDS             complement(1393..>1897)
                     /locus_tag="PK612_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95221.1"
                     /translation="DNNYVVVGGDFNHDLVLDNPLVNEEYKANIFNKQETEILEANWY
                     NYFRLDSGKHGTRVPNIITGLEEDYTHDFYDMALSSHGPVNIATCRDASIKFIDKNNN
                     GIVDNAMVTIDGFLVSDNIAVTEIKTIGSGPGLQKENLPESDPRHGLGFVYSDHNPVL
                     MSFKLQA"
BASE COUNT          645 a          339 c          349 g          564 t
ORIGIN      
        1 aggtttaagt aatgtaatgc ggaagtttga acttccgaga ggtatccctt atatctttgc
       61 taaaccgcgt atggaactat atgtacaaaa atcaactgaa tttaatgccc tcttacttaa
      121 ttattttgca aaggaagact ggtatcctta ttcgattgac gaaactttta ttcatttaac
      181 accgtactta gaactttata agaagacacc agaagaactt attgaattta ttattaaaga
      241 aatatatgat cacttaaaac tctatgtaac cgtcggtata gcgccaaata tgtttttagc
      301 taaatgtgta cttgatttag aagccaaaaa agacccacgc tattacgcaa gatggacaat
      361 ggaagatgtt aaaactaaat tgtggcaaat taaaccactg cataaaatat ggggtattgg
      421 taaacgctat gaaagaagac tccataaatt agggcttttt acaatggggg acattgctaa
      481 gtgtccgaaa gcgaagttaa aagaagaatt tgggattatg ggtgaagagc tttgggaaca
      541 cgctaacggg attgataaca ccgacttaag tgaaaagtat gtcccacttg ataagtcact
      601 aacaagtgga caagtgttat tccgtgatta cgaagttaac gaagcacccg ttttaattcg
      661 tgaaatgact gatgaattat gtatgcgtat gcgtaaaaat aatttaaaag ctaaaaaagt
      721 tggtttaagt attatgacta gtaagccaga aagtaataca tatagtaaat caatgtcact
      781 tcttcttcca acatgctcca atactgaact atttaaagct ttcatggaag tatttaataa
      841 gtttccttta gaaggtgcta aaatacgctc tcttcatctt agcgtcagcg gactttcaag
      901 cgacccttat gagcaactta gtctcattga ggattatgta acacaaaaca ctaaagagca
      961 aagaatgtgg gaaacacttg atgaacttca cgcgcgttac gggaaagata aaataatgcg
     1021 cgcgaacgca ctcacgcgtg cgagcactta tttacgacgc actaaacaaa ttggaggtca
     1081 caataaatga gtcgcgggat gaaaaagttt ttaccgtttg caagtctaaa agaacaggca
     1141 cttttcctag cggaaatgca gcagcaaaaa aagcgtgttg aaaagcccac actcagtgaa
     1201 gaccaatgcg cagaaattaa tcgtgtttta agcgaataca ccggcgaaac cgtattaatt
     1261 tattattata accgcggttt tattaagacg catcaagcca aaataaaaaa gattgatact
     1321 ttatataaac gcttagtgtt tactgaccta accgtttcat ttaccaatct tttacatatt
     1381 gaaattattt aattacgctt gtaatttaaa actcataagc acagggttat gatcactata
     1441 aacaaaccct aacccgtgac gcgggtcgct ttcgggtaag ttttcttttt ggagaccagg
     1501 gccactacca atcgttttaa tttcagttac cgcgatatta tcactaacaa ggaaaccatc
     1561 aattgtgacc attgcattat caacaatccc gttattattt ttatcaataa atttaattga
     1621 ggcgtcgcga catgtggcaa tatttaccgg gccgtgacta gatagcgcca tatcataaaa
     1681 gtcatgcgta taatcttctt ctaaaccggt aataatgttt gggactcttg ttccgtgctt
     1741 tcctgagtca aggcgaaaat agttatacca attcgcttca agaatttctg tttcttgttt
     1801 attaaagata tttgctttat attcttcgtt aacaagcggg ttatcaagca ctaagtcatg
     1861 attaaagtcg ccaccaacga caacatagtt attatct
//
LOCUS       DAOSEX010000174         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_61419_length_1896_cov_4.800652, whole genome shotgun sequence.
ACCESSION   DAOSEX010000174 DAOSEX010000000
VERSION     DAOSEX010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61419_length_1896_cov_4.800652"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PK612_03585"
     CDS             <1..720
                     /locus_tag="PK612_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95222.1"
                     /translation="IKYKEVYPDGYNFQTGFYSITSFTKSKVDSSDYFEIETYNIKAQ
                     LQSKPYGVSLKFETNMMQLNRSLVLGAGREGPIENFENILWDGEYLHKTIKYNNGYVP
                     KETWTEKTYGTNQIETLQINTEIDKIDYIISTLETKLTDLEKHVVNKSNVNFKNNSLI
                     VSTFFSKLETYKIIRIDFDENFVISRYQISYTDLGRVPDELRLRQITQILKPIDAIEV
                     NAQLEYEEELIVEKDIFTIQI"
     gene            861..>1896
                     /locus_tag="PK612_03590"
     CDS             861..>1896
                     /locus_tag="PK612_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH95223.1"
                     /translation="MLVLKDIYKDYYVAKKPLHVLKGLNLTFPKQQFVTILGPSGCGK
                     TTLLNLIGGLDKYTSGDLIIEGKSTKEFKDRDWDAYRNHRVGFVFQSYNLIPHLSVIE
                     NVEISLTLSGMGSSERREKAKQALLSVDLESELYKRPNQLSGGQLQRVAIARALVNDP
                     SIILADEPTGALDSETSEQVMKILKEISKTRLVVMVTHNEDIAKRYSDRVIRMLDGRI
                     TSDTDEAQSKKKRKVKEPTETEKNKGTSMSFLTALKNSYKNILTKKGRTVFTALAGSL
                     GIIGIGLVAAVSNGFQNYINRLERETMANYPIAVFAQTMDMQAGIKKPDKNTEYPDGE
                     EIVVYNENDTE"
BASE COUNT          741 a          260 c          344 g          551 t
ORIGIN      
        1 attaaatata aagaagtgta tccagacgga tataattttc aaacaggatt ttatagtatt
       61 acatcattta caaaatcgaa ggttgatagc tctgattatt ttgaaattga aacatataac
      121 attaaggctc aactgcaatc aaaaccgtat ggtgtatcat tgaagtttga aacaaatatg
      181 atgcagttga atcgttcttt agtgttgggt gcaggaagag aaggccctat tgaaaatttt
      241 gaaaacatat tatgggacgg agaatatttg cataaaacaa taaaatataa caacggttat
      301 gtacctaaag aaacatggac tgaaaaaaca tatggcacaa atcaaataga gacattacaa
      361 ataaatactg aaatagataa aattgattat attataagta ctcttgagac taagctgact
      421 gatttagaaa aacatgttgt taataaatca aatgttaatt ttaaaaacaa ttcgttgatt
      481 gtttctacat ttttcagtaa attagaaaca tataaaatta tacgaattga tttcgatgaa
      541 aattttgtaa tttcacgata tcaaatttca tatactgact taggtagagt tcctgatgaa
      601 ttaagactaa gacaaataac acaaatactg aaaccaatag atgcaattga agttaacgct
      661 caattagaat atgaagaaga acttatcgtg gaaaaagata tatttacgat tcaaatttag
      721 tatagtaaca aagattaact aaattcccgt ctcttgtaaa accgcaatta taaaattaat
      781 gtaaataata atgcctttat cttgaaaaga taaataaaaa gttagacaat gtataaagtg
      841 aaatccgaaa ggggatattt atgcttgttc tcaaagatat ttataaagat tattatgtgg
      901 ctaaaaagcc acttcatgtt ttaaaggggc taaaccttac ttttcctaaa caacaattcg
      961 taacaatttt aggtccatcg ggttgtggta aaacaacact tttaaacttg attggcggtc
     1021 ttgataaata tacatcaggc gatttaatca ttgaaggtaa atcaacaaaa gaatttaaag
     1081 atagagattg ggacgcttat cgtaatcatc gtgttggatt tgtgtttcaa agttataatc
     1141 ttatcccgca cttaagtgtg attgaaaatg ttgagatttc tttaacgctt tctgggatgg
     1201 ggagtagtga acgtcgcgaa aaggctaaac aagccctcct tagtgttgat cttgaaagtg
     1261 aattatataa aagaccaaat caactttctg gtggtcaact tcaaagagta gcgattgcac
     1321 gtgcacttgt taatgatcct tccatcattt tagcggatga acccaccgga gcgttagata
     1381 gtgaaacaag cgaacaagtc atgaaaattt taaaagaaat tagtaaaaca cgacttgttg
     1441 taatggttac gcacaatgaa gacatcgcta aacgatatag cgatcgagta attaggatgt
     1501 tagatggcag aattactagt gacactgatg aagcacaaag taaaaagaag cgcaaagtaa
     1561 aagaaccaac ggaaacagaa aaaaacaagg gaacatcaat gtccttttta accgcgctta
     1621 aaaacagtta taaaaacatc ttaacaaaaa aaggaagaac agtgtttact gcgcttgcag
     1681 gatcattagg gattattggt attgggttag tagcagcggt tagtaacgga tttcaaaatt
     1741 atattaatcg tttggagcgg gaaacaatgg caaattaccc aattgccgtt tttgcgcaaa
     1801 caatggatat gcaagcaggc ataaaaaaac ctgataaaaa cactgagtat ccagacggtg
     1861 aagaaatcgt tgtttataat gaaaatgata cagaaa
//
LOCUS       DAOSEX010000175         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_61432_length_1896_cov_3.080934, whole genome shotgun sequence.
ACCESSION   DAOSEX010000175 DAOSEX010000000
VERSION     DAOSEX010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61432_length_1896_cov_3.080934"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1896)
                     /locus_tag="PK612_03595"
     CDS             complement(<1..>1896)
                     /locus_tag="PK612_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95224.1"
                     /translation="LAQYRLATLGYDIGSKFIEGWGTSYTYPSGWWHTTEKTYIENIL
                     RVMDLHIAPLGINAFNIVRDAFNKGSIITPKSTTNYHENIISPFGDDSTEGALKFKGL
                     FFEIDYNGFYNGTLIHSKDNDFGSITINDNPSSSLTILESDGVFQKEKLNRLGNEIVA
                     LAGKYTSLAQVQDIGSVFDDDVVIFKSEISVGNNEVRANYFGSKDYVMKNYFTSVYSK
                     HRTTNLIPYGESIVRAENKKVFMFLSKTDYIVDNTALNFNFQYFTNIEFDILSAFRPS
                     EILTSKDDFKNSHKINLGFFYKGGKAYLSDIARFVSGNSIGFNIRMYDNVSGGVYIKT
                     KEAGEDGTAGSEQAWHLLVDDIETGYIESLSVYFGHIDAQTQFAEMPLEYSRSIVIDA
                     YDRMFNLPEQNISLTSSKNLMGGNLLVNKDNKEVLDYTFQIETITDDDNIIFTPLFMG
                     MSDLIGNYHKVERDYSVSDIKGGGFIEQVYASSFAEMISGNRIPYITLQLNVPHTYET
                     NLNVNDRVEFIATWDFKQEMGAKIIAEQITGLEAVMSGDQVIGMGKITIRVKVLWRDV
                     YGTWYNRGQKYLTFKRVGNYFTESENPLFYNQPHRDGTTSSFGVRVLCEDLVNIIDAD
                     FNNDKFG"
BASE COUNT          577 a          407 c          259 g          653 t
ORIGIN      
        1 cgccgaattt atcgttatta aaatcagcgt caattatatt aactaaatct tcacataata
       61 cacgcacacc aaaagatgaa gtcgtgccat ctctatgcgg ctgattataa aacaacggat
      121 tttcgctttc agtaaaataa tttcccactc gcttaaatgt aagatatttt tgccccctat
      181 tataccaagt gccgtaaaca tctcgccaca atacctttac tcttatcgta atctttccca
      241 tacctattac ttggtcgcca ctcattactg cttctaaccc tgtaatttgt tccgcaatta
      301 ttttcgcacc catctcttgc ttaaaatccc aagttgcgat aaattcaact cgatcattca
      361 catttaagtt agtttcgtat gtatgcggaa catttaattg taaagtaatg taaggaatac
      421 gatttccgct tatcatttcc gcaaaacttg atgcataaac ttgctctata aatccaccac
      481 ctttaatatc gctaaccgaa taatctcgtt ctaccttatg ataattacct atcagatcgc
      541 tcatacccat aaacaaagga gtaaaaataa tattgtcatc atcggttatt gtttcaattt
      601 gaaaagtgta atccaacact tctttattat ctttattaac aagtaaatta ccacccatca
      661 agtttttact acttgtcagc gatatatttt gttcgggtaa attaaacatt ctatcgtaag
      721 catcaataac gattgaccta gaatattcta atggcatctc ggcgaattga gtttgtgcat
      781 caatatgtcc aaaatataca cttaaacttt caatataacc cgtttctata tcatccacca
      841 gtaaatgcca cgcttgttcg ctcccagccg ttccatcttc tccagcttct ttcgttttga
      901 tatatacacc gcccgacaca ttatcataca ttcttatatt aaaacctatt gagttgccac
      961 taacaaatcg tgctatatcg cttaaatatg ctttaccacc cttgtagaaa aaacctaaat
     1021 ttattttgtg cgaatttttg aagtcatctt ttgaagttag gatttcgctt ggtctaaacg
     1081 cactcaatat atcaaattca atgttagtaa aatattgaaa attaaagtta agcgcagtgt
     1141 tatccacaat ataatcggtt ttagataaaa acataaagac ttttttattt tccgctctaa
     1201 ctatactttc cccatacgga ataagatttg tcgttctatg cttactatat acgcttgtaa
     1261 agtagttttt catcacataa tctttcgagc caaagtaatt tgctctcact tcgttattcc
     1321 caactgatat ctcgcttttg aaaatcacga catcatcatc aaaaactgaa cctatatctt
     1381 gcacttgagc gagagaagta tattttccag ccaatgcgac tatttcattt cccaagcggt
     1441 ttaatttctc tttttgaaaa acgccatcgc tttcgagtat cgttaaactt gaactcggat
     1501 tatcgtttat tgtaatactg ccaaaatcgt tatctttcga gtgaattaaa gttccgttat
     1561 aaaacccgtt ataatcaatt tcaaaaaata accctttgaa ctttaatgca ccttcggttg
     1621 aatcatcgcc aaatggactg atgatatttt catgatagtt tgtggtgctt ttcggcgtga
     1681 ttatcgaccc tttgttaaat gcatctcgaa caatgttaaa agcgtttatg ccaagtgggg
     1741 cgatatgtaa gtccatcact cgtaaaatgt tctcgatata ggttttttcg gtagtgtgcc
     1801 accacccact tggataggta taacttgtcc cccacccttc aatgaatttt gaaccgatat
     1861 catagcctaa agtcgccaat cgatattgtg ctaatt
//
LOCUS       DAOSEX010000176         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_61653_length_1892_cov_2.800218, whole genome shotgun sequence.
ACCESSION   DAOSEX010000176 DAOSEX010000000
VERSION     DAOSEX010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61653_length_1892_cov_2.800218"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..>1892
                     /gene="mutL"
                     /locus_tag="PK612_03600"
     CDS             139..>1892
                     /gene="mutL"
                     /locus_tag="PK612_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOH95225.1"
                     /translation="MAKIILLDNELANQIAAGEVIERPSSVVKELVENSIDAGATKID
                     VFINEAGRKAIIVQDNGSGIPSDELALAFSRHATSKIHSTFDLFRIRTLGFRGEALPS
                     IASIAKVQVETAEKGKPGALAKIENGDIKIEPSAVSSGSKITVSELFYNTPARLKYLK
                     RDYTENAVTLDVVTKIALAHPDVSFSLTFDDKLRFRTSGRNNLEETILNIYGLTCVKN
                     LLPINVKTNDFTITGFLGNTNLVRSNRYGIITNLNGRNVYMPKVQSTIIEAYHGFIAP
                     TRYPFVILNLQIEPELVDVNVHPTKKEVRFSKEEDLRNVLLKEIPLALRKEAFIPKDP
                     HQKVQPLSSTTNEEPRAEQLIFDLPFGTETSELKISDSHHQEAIRKPITKTISELENS
                     FVNPVAALHNTYIICEVNDGGFWLIDQHAAHERINYEKFQKVLNMDLKTRPLLVPELL
                     SFSHSEIALFTPEVFTILKDVGIEAELFGPNTLRVTAVPNWAGEYDERIYIQDIIDAV
                     ITDKRLNQELLRANAIATMACKASIKANHRLSIMEMQHLIQELLACDNPFSCPHGRPT
                     IIKFTKYELEKMFKRTGT"
BASE COUNT          660 a          302 c          353 g          577 t
ORIGIN      
        1 cggcaatccg actaaaacag aatatgtgcc actaaaacca ttatggtatc aacagctcga
       61 aaatttaaat attgatgaaa tgactcccct agatgccctt attcttttaa agaaaattaa
      121 agatgaaggg tttaaataat ggctaagatc attttactag acaacgaact tgctaatcaa
      181 attgcagcgg gggaggtaat tgaaagacca agttcagttg ttaaagaatt agtggaaaat
      241 agtattgacg ctggtgctac taaaattgat gtttttatta atgaagccgg acgtaaagca
      301 ataattgtac aagataatgg tagcgggatt cctagcgacg aattagcgct agctttttcg
      361 cgacatgcaa ctagtaagat tcattcaact tttgatttat ttagaataag aactttaggg
      421 tttagaggtg aagcactacc aagtattgct tcaattgcaa aagtgcaagt tgaaactgct
      481 gaaaagggga aacctggagc acttgctaag attgaaaatg gtgatattaa aattgagcca
      541 agtgcagtta gtagtggttc aaaaattact gtctctgaac ttttttacaa tacaccagca
      601 cgccttaaat atttaaaacg cgattatacc gaaaacgctg taacacttga tgttgtgact
      661 aaaattgctt tagcccatcc tgatgttagt tttagtttaa catttgatga taagttaaga
      721 tttcgcacaa gtgggcgaaa taatttagaa gaaacaattt taaatattta tggtttaaca
      781 tgcgttaaaa acttattacc aattaatgtt aaaacgaatg attttacaat tacaggtttt
      841 ttagggaaca cgaatttagt tcgttctaat cgttacggga tcattacaaa cttaaatggt
      901 cgtaatgtat atatgccgaa agttcaaagt acgataattg aagcgtatca cggctttatt
      961 gctcccacgc gttatccgtt tgtcatttta aatttacaaa tagaaccaga attagtcgat
     1021 gttaatgtgc atccgactaa aaaagaagtg cgtttttcaa aagaagaaga cttacgcaat
     1081 gttttattaa aagaaattcc gctagcgctc cgtaaagaag catttatccc taaagatccg
     1141 caccaaaaag tgcaaccact atcaagcact actaacgaag aacctagagc cgagcaactt
     1201 atttttgatt tgccgtttgg cactgaaact agtgaattaa aaattagtga tagtcatcat
     1261 caagaagcaa ttagaaaacc aataactaaa acaattagtg aattagaaaa tagttttgtt
     1321 aatccagttg cagcactcca caacacttac attatttgtg aagtaaatga tgggggcttt
     1381 tggcttatcg accaacacgc tgctcatgaa cggattaatt atgagaagtt tcaaaaagta
     1441 ttaaatatgg atttaaaaac tagaccactt ttagtgcctg aacttttatc ttttagtcat
     1501 agtgaaatag cgctttttac tcctgaagta tttaccattc ttaaagatgt cggaattgaa
     1561 gcagagttat ttggcccaaa tacactaaga gtgactgctg taccaaattg ggctggggag
     1621 tatgatgaac gtatttatat tcaagatatt attgatgccg ttattactga taagcgttta
     1681 aatcaagagc ttttacgcgc taatgctatt gcaacaatgg catgtaaagc atcaattaaa
     1741 gctaatcacc gcctttcaat tatggagatg caacatttaa tacaagaatt acttgcgtgc
     1801 gataatccgt ttagttgtcc gcatggtcgt ccaacaatta ttaagtttac gaagtatgaa
     1861 ttagaaaaaa tgtttaagag gactggcaca tg
//
LOCUS       DAOSEX010000177         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_61892_length_1888_cov_2.316967, whole genome shotgun sequence.
ACCESSION   DAOSEX010000177 DAOSEX010000000
VERSION     DAOSEX010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61892_length_1888_cov_2.316967"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="PK612_03605"
     CDS             complement(<1..1452)
                     /locus_tag="PK612_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95226.1"
                     /translation="MQDIINFILENLPRVILLSGTIVMLVIAVLLIIHGNRKDLKDQN
                     IILGNITTPRIFLLDLGEGKVTYFNRGSFGERKNGLLPQFFQQFTAKEVDRLDKWVNA
                     LIDPKSEASKFYKVDVFVRDMRRSYASILEVTGVDYEKKIIHLESYLYRYLKANDQMN
                     LTVKKKENIGYLTEEELVKVYRRAHKRGRGVYGAISIAVTASGAQTHRAEIPPHLWYE
                     LQNQVAVYRKRSRVIRFVDNHTVGIYFPRSSDVDTAIDVFQSIKKRMSGYLQKNGYNE
                     YSLGIGLTRSRQFSEMNMFFKTAEELSLYALKEDGRLLIHDPNKEIKDMDLSLYRSEL
                     TQLVARKGVDTLFQPIVNAKTVEILGYLTTFSISGSLFGSFNEAAQFARENKQNEQLV
                     KMVLRKAAARYYNMRRNNKQLLFVPLGLGGVDFISDAFSTQPFANDLKMVFMLEEQDF
                     ANTFVNIEDMLNLIKNIKKQKYEVCLIISDTELT"
     gene            1551..1643
                     /locus_tag="PK612_03610"
     tRNA            1551..1643
                     /locus_tag="PK612_03610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1587..1589,aa:Ser,seq:cga)
     gene            1660..>1888
                     /locus_tag="PK612_03615"
     CDS             1660..>1888
                     /locus_tag="PK612_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOH95227.1"
                     /translation="MAKKRLLIVVDYQNDFVNGALGFSDAHEIEQKIIDTIEDHLKTK
                     DDIVFTKDSHFDNYLETEEGRNLPFKHCIKGT"
BASE COUNT          590 a          375 c          318 g          605 t
ORIGIN      
        1 tgttaattca gtatcactaa tgattaaaca cacttcatac ttttgttttt taatattttt
       61 aataaggttt aacatatctt caatgttcac aaatgtgtta gcgaaatctt gttcttctaa
      121 cataaagacc attttgagat cattcgcaaa tggctgcgta ctaaaagcat cactaataaa
      181 gtcgactcca cctaacccaa gggggacaaa tagtaattgc ttattattgc ggcgcatatt
      241 gtaataacgg gccgctgcct tccggagcac catctttaca agctgttcgt tttgcttatt
      301 ttctcgcgca aattgcgcag cttcattgaa actcccgaat aatgaaccgc tgatgctgaa
      361 ggtcgttaag taccctaata tttcaactgt tttagcatta acaattggtt gaaataaagt
      421 atcgactccc ttgcgagcca caagttgtgt tagttcactg cgatataaac ttaagtccat
      481 gtctttaatt tctttatttg gatcatgaat taagagccga ccgtcttctt taagcgcata
      541 aagacttaat tcttccgctg ttttaaaaaa catattcatc tccgaaaatt gacgagaccg
      601 cgttaaccca atcccgagtg aatattcgtt gtagccattt ttttggagat aaccgctcat
      661 gcgtttcttt attgattgga acacatcaat agcggtatca acatcacttg aacgcgggaa
      721 gtatatgcca acagtatgat tatcaacaaa acgaatgacg cgacttcgtt tccgataaac
      781 agcgacttgg ttttgtaact cataccataa atgcggcggt atttctgcgc ggtgcgtttg
      841 cgctccgctt gctgtcactg caatactaat cgcaccgtat acgccacgcc cgcgcttatg
      901 ggcgcgtctg tataccttta ctaattcttc ttcagttaag taacctatat tctctttttt
      961 cttcaccgtt aaattcattt ggtcattagc ctttaaatat cgatacaaat aagattcaag
     1021 gtgaataatc tttttttcat aatcgacacc agttacttct aaaattgaag cgtatgaccg
     1081 acgcatatct cgcacaaaaa catcaacttt ataaaattta cttgcttcac tttttggatc
     1141 aattaaagcg ttaacccatt tatcaagtcg atcaacttct ttcgcggtaa attgttgaaa
     1201 aaattgtggt aataaaccat ttttgcgttc gccaaaacta ccgcgattaa agtaagtgac
     1261 ttttccttca cctaagtcaa gaagaaaaat ccgcggtgtt gttatatttc ctaaaataat
     1321 gttttggtct ttaagatctt tacgattacc gtgaataatt aaaagcacag caataactaa
     1381 catcactatt gtgccgctaa gtagtattac ccttggtaag ttttctaata tgaaattaat
     1441 aatgtcctgc ataaatcacc tatgtatatt atacataaaa ataattttat cattgcgtag
     1501 aagcgaatat tttatagata ctataaaatc tttgttataa ttagcattac ggagaaatac
     1561 ccaagcctgg ctgaaggggt cggtctcgaa aaccgatagg aggagcaatc ttcgcgaggg
     1621 ttcgaatccc tctttctccg ccatgaagga ggaaaacaaa tggcgaaaaa acgtttatta
     1681 attgttgtgg actatcaaaa tgacttcgtt aatggagcgc ttggctttag tgatgcccat
     1741 gaaatcgaac aaaaaatcat tgatactatt gaagaccact taaaaacaaa agatgacatt
     1801 gtcttcacta aagacagcca ttttgataac tatttagaaa ctgaagaagg gcgtaattta
     1861 cctttcaaac actgtatcaa aggtacac
//
LOCUS       DAOSEX010000178         1887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_61943_length_1887_cov_2.267467, whole genome shotgun sequence.
ACCESSION   DAOSEX010000178 DAOSEX010000000
VERSION     DAOSEX010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61943_length_1887_cov_2.267467"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="rsgA"
                     /locus_tag="PK612_03620"
     CDS             complement(<1..776)
                     /gene="rsgA"
                     /locus_tag="PK612_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOH95228.1"
                     /translation="MAGLVINKQKSSFIVEKNGETYVTKPSGNLIKSRQTIYVGDYVE
                     IDTENNLISELLPRRNFLVRPPIANVDIVLIVMSMVQPDFSLFLVEKFLSYANFAEIK
                     PLVIITKQDKLDTDDLLKEVQDSLDKLHVKNYVVNNKSGEGLDAVRKYLHGKVVALMG
                     QSGVGKSSFINALTDYDREVGKFSISINRGRHVTKEVIMLPFNAGYLVDTPGFSSFEL
                     PLTKGDLAENFPGFKKYIGTCKFNNCLHLSEIGCTVKADV"
     gene            complement(777..1664)
                     /locus_tag="PK612_03625"
     CDS             complement(777..1664)
                     /locus_tag="PK612_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOH95229.1"
                     /translation="MIRIGDLIDNRYRIVSLLGEGGMGTVYEAVDIITKRNVALKFLK
                     ANVARDKVNLARFEIEAKATATLNHPNIVRVISVGKYENLPYMVNELIVGNNLEDELQ
                     TRGRYTFLEAADIIDQLCNAVSLAHKNGVIHRDIKPSNIYITKEGLVKLGDFGIARYS
                     DSKRVTSNTNIVGSAHYLAPEVCRGGEASPRSDIYALGINFFELITGHVPFDGDSNVA
                     IAVKQIKEKFPSLRKYVKNTPKAIEKIIKKCCKKNPRERYPDVESLQQDILQLKTNPK
                     LLRPRQSIWVRWFGFVSED"
     gene            complement(1657..>1887)
                     /locus_tag="PK612_03630"
     CDS             complement(1657..>1887)
                     /locus_tag="PK612_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase"
                     /protein_id="HOH95230.1"
                     /translation="GTMPTVTFDFRKFQYRGERILVCSDGLYNMLDSEDILGILRTTQ
                     TAEFKVDALVALANHRGGADNIGVALWEPDND"
BASE COUNT          579 a          404 c          301 g          603 t
ORIGIN      
        1 tcaacatcgg ccttaacggt acagcctatt tcactaagat ggagacaatt attaaattta
       61 caagtaccaa tatacttttt aaatcccggg aagttttcgg caaggtcgcc tttcgttagc
      121 ggtaattcaa aggaagaaaa gcctggtgta tcaactaaat atccggcgtt aaacggaagc
      181 attattactt ctttagtgac atgtcgacca cggttaatgc taattgagaa cttaccaact
      241 tcgcggtcat aatctgttaa agcgtttata aaacttgatt ttccgacacc agattgcccc
      301 ataagtgcca caactttgcc atgcaaatat ttgcgcacag catcaagtcc ttcaccactt
      361 ttattattga caacataatt tttaacgtgt agtttatcaa gactatcttg tacttccttt
      421 aacaagtcat cagtgtcaag tttatcttgc tttgtaatga tgactagtgg cttaatttcc
      481 gcaaaattag cgtaactaag aaacttttcg actaaaaata gactgaaatc aggttgcacc
      541 attgacatga caattaacac aatatcaaca ttggcaattg gtggtcttac taaaaagttt
      601 cgccgtggta gaagctcact aattaaatta ttttccgtat caatttcaac ataatccccg
      661 acataaatag tctggcgcga tttaattaaa ttaccgcttg gtttagttac ataagtttca
      721 ccatttttct ctacgataaa acttgatttt tgtttattaa taactaatcc agccatctag
      781 tcctcagaga caaacccaaa ccagcgcacc caaattgatt gccgtggccg taaaagtttt
      841 gggttagtct taagttgtaa tatatcttgt tgtaatgatt caacatcagg atagcgttcc
      901 cgtggatttt tcttacaaca ttttttaata attttctcaa tagctttcgg ggtattttta
      961 acatacttac gcagacttgg aaacttctct ttaatttgtt ttaccgcaat tgccacattc
     1021 gaatcaccgt caaagggaac atgacctgta attaattcaa agaaattaat acctaaagca
     1081 taaatatcac tgcgtggtga agcttcgcca ccacgacaca cttcgggcgc taagtaatgg
     1141 gcgctaccaa caatatttgt gttacttgtg acacgctttg agtcactata acgagcaata
     1201 ccaaagtccc caagtttaac aagtccttct ttagtaatat aaatattact tggttttata
     1261 tcacgatgaa ttaccccgtt tttatgcgct aaactaacag cgttacacag ctgatcaata
     1321 atatcagcag cttctaaaaa tgtatagcga ccgcgcgttt gcagttcatc ttctaagtta
     1381 ttaccgacta ttaattcatt caccatgtaa ggtaaatttt catacttgcc tacactaatg
     1441 acacgaacaa tatttggatg atttaaagtc gcagttgctt tggcttcaat ttcaaaacgg
     1501 gcaagattca ctttatcacg agcgacatta gcctttaaaa acttcagcgc gacattacgt
     1561 ttggtaatga tgtcgactgc ttcataaact gttcccattc cgccttcacc aagcaaactt
     1621 acaatccgat atcgattgtc aattaaatca cctattctaa tcattatctg gctcccataa
     1681 tgctactcct atattgtcgg ctccaccgcg atgattggcg agagcgacga gtgcgtctac
     1741 tttaaattcc gctgtttgtg ttgtgcgtaa tattcctaaa atatcttcgc tatcaagcat
     1801 gttgtaaaga ccatcactac acactaaaat tcgttcaccc cgatattgaa acttcctaaa
     1861 atcaaaagta acagttggca tcgtacc
//
LOCUS       DAOSEX010000179         1884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_62073_length_1884_cov_4.264079, whole genome shotgun sequence.
ACCESSION   DAOSEX010000179 DAOSEX010000000
VERSION     DAOSEX010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62073_length_1884_cov_4.264079"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PK612_03635"
     CDS             complement(<1..724)
                     /locus_tag="PK612_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95231.1"
                     /translation="MSGFIGRVGNSCVKEKLLSALNLLQFKDDDSTGVLFCNKEGQHL
                     YRVVGKVDELVTLIPEEVDGYMGIAHTRWSTYMHQVLENVYPLSSENGYVTLVVNGLI
                     DNIQMIKRRLVKRGYSFRSSSAVEVVANLIEDGAVENEDPLRALHRAMQQLEGDYALL
                     AVFNGNCKKIFFAKNGVPLIIGKNHDGMVICTDYTPIADQADEYYYPNDGELGYLTSD
                     NMVLYDGKLKQRKPSFTEVSVSE"
     gene            complement(779..1441)
                     /locus_tag="PK612_03640"
     CDS             complement(779..1441)
                     /locus_tag="PK612_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95232.1"
                     /translation="MLKSCLELFDEIANIFNKHGFKLYMVGGTSRDFLLNYPVDDYDF
                     ATDATPTEMHEFLPDANYRFEKFGTVSLKYEDEHVEITTLREEGEYIDSRHPAYIKFV
                     TDPAKDYIRRDFTINALYIDENYRVLDFCDGLKDLDTNTLRVIGNPYTRFAEDPLRII
                     RALRFVAKFKLNIDPLTENAIRDLAHLVKKLNPEKVKAEIRKVGEQEQNIFKKLMNMY
                     KL"
     gene            complement(1463..1735)
                     /locus_tag="PK612_03645"
     CDS             complement(1463..1735)
                     /locus_tag="PK612_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOH95233.1"
                     /translation="MNKAEIIAAIADELMITKRSVEEIINLFCDTVIETLAKGEDVKL
                     TGFGTFQTRERKERRGVSPQTGTEIVIPSGRTIVFKPSKLLKEKVQ"
BASE COUNT          581 a          377 c          293 g          633 t
ORIGIN      
        1 tttcactaac tgacacttcg gtaaaagacg gttttctttg tttaagtttg ccgtcatata
       61 aaaccatatt atcacttgtt aaatagccta actctccgtc atttggataa taatattcat
      121 cggcttgatc ggcaattggg gtgtaatctg tacaaatgac catcccgtca tgattctttc
      181 caataatgag cggaacacca tttttagcaa aaaatatttt cttacaatta ccgttaaaga
      241 cagcaagcaa ggcataatca ccttctagtt gttgcatagc gcgatggagt gcccgtagtg
      301 ggtcttcgtt ttcaacagcg ccgtcttcaa ttaaattagc gacaacttca acagcggaac
      361 tagatctaaa actatatccg cgtttcacaa gtcgacgctt aatcatttga atattatcaa
      421 taagcccatt aacaacaagg gtgacataac cattttcact agagagcgga taaacatttt
      481 caagaacttg atgcatgtat gttgaccatc tagtgtgagc aatccccata taaccatcaa
      541 cttcttccgg aataagtgtg actaattcat ctacttttcc tactacacga tataaatgtt
      601 ggccttcttt attgcaaaaa agaactcctg tggagtcatc atctttgaac tgaagtaagt
      661 ttaacgcact taacagtttc tcttttacac acgaattacc aactcttcct ataaatccac
      721 tcataaagat tccctcactt ttggtattaa tagtttacac tattacgcaa tgtaaatctt
      781 ataatttgta catattcatt aattttttaa agatattttg ctcttgttct cccacttttc
      841 gtatttcagc tttaactttt tctggattta gttttttaac aagatgcgct aaatcacgaa
      901 tcgcgttctc agttaagggg tcaatattta atttaaactt agcaacaaat cgcaatgccc
      961 gaataatccg cagcggatct tctgcaaaac gggtataagg attgccaata acgcgtaaag
     1021 tatttgtatc taaatctttt aagccatcac aaaaatcaag cactcgataa ttttcatcaa
     1081 tataaagtgc gttaatcgta aaatcacgtc taatataatc tttagcgggg tctgttacaa
     1141 actttatata agcggggtgg cgactatcaa tatattcacc ttcttcgcgc aaagttgtaa
     1201 tttccacatg ttcatcttca tactttaaac ttactgtccc aaacttttca aaccgataat
     1261 ttgcgtcagg caaaaattca tgcatttccg ttggtgtagc gtctgtcgca aaatcatagt
     1321 cgtcgaccgg gtagtttaaa agaaaatcac ggcttgtccc tccaaccatg tagagtttaa
     1381 agccgtgctt gttgaaaata ttagcaatct cgtcaaaaag ctctaaacat gatttcaaca
     1441 tacttctcta gcaaagagtt atttattgaa ctttttcttt gagaagtttt gaaggtttga
     1501 aaacgatagt acggccgctt ggaataacaa tttccgtacc ggtttgtggg ctaacgccac
     1561 gacgttcttt acgttctctt gtttggaaag tgccaaaacc tgtaagtttt acatcttcac
     1621 ctttcgctaa ggtttcgatg actgtgtcac aaaataaatt aataatttct tccacagaac
     1681 gcttagtaat cataagctca tcggcaatgg cagcaattat ttctgctttg ttcatattat
     1741 tttctcactc ctttgttttt tacaaagtag cataattata taatttatac aagaaatatt
     1801 taatatttta ttgaaaatta ttgcgatgct gtactgatac cctaaatgta gacacacaat
     1861 aaaatcatgt ggccacatga tttt
//
LOCUS       DAOSEX010000180         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_62343_length_1879_cov_3.560855, whole genome shotgun sequence.
ACCESSION   DAOSEX010000180 DAOSEX010000000
VERSION     DAOSEX010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62343_length_1879_cov_3.560855"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PK612_03650"
     CDS             complement(<1..308)
                     /locus_tag="PK612_03650"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Enables the production of acetyl-CoA by
                     phosphorylating acetate in the presence of ATP and a
                     divalent cation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOH95234.1"
                     /translation="MSKLVLAINSGSSSLKFKLFKVPSEKVITSGLFERITQKMGAFR
                     IKTETGEVRRELPLPNHEVAVNVLTKALIEFKIVKSLDEINAVGHRVVQGGKYYNDSV
                     "
     gene            677..>1879
                     /locus_tag="PK612_03655"
     CDS             677..>1879
                     /locus_tag="PK612_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH95235.1"
                     /translation="MSFIDEFLMGFTTSAHKYFGAHFNPAKGKKKKSVTFRLYAPAAS
                     DVSVIGDFNGWDPNVHKMEKVDYRGIWEITIPELENYQNYKFRFRNAHGHYVDKADPY
                     AFFSEKAPGTASRLFDYEGFIWHDEDWMKSRCRGHDKPISIYEIHLGSWKGKENGRIL
                     SYEEIADYLIPYMRDNGFTHVEIMPITQYPFDGSWGYQATGFFSVDSRYGNPMQLMSF
                     VDRMHQAGFGVILDFVAVHFAIDHYGLIEFDGTRMYEYQDSEHTFSQWGSPQFDLGRD
                     PVRSFLISAIFYFLEYFHFDGIRIDAVSNIVYWHGNSDRGVNHGGVEFLKRLNGKVHY
                     HFPGVMMIAEDSTAFDGTTRDVEWGGLGFDYKWDMGWMNDTLRYYSKDPIYKKYDHNL
                     FTFSMHYFF"
BASE COUNT          563 a          315 c          363 g          638 t
ORIGIN      
        1 tcaacgctat cattgtaata tttaccgcct tgaacaacac ggtgacccac tgcgttaatt
       61 tcatctaatg atttaacgat tttaaattca attaaagcct tcgttaaaac attaaccgct
      121 acttcgtgat ttggtagtgg tagttcgcgt cttacttcac ccgtttcggt tttaatgcga
      181 aacgctccca ttttttgtgt aattctttca aaaagtccac ttgtaattac tttttcactt
      241 ggaactttga aaagtttgaa ttttaaggaa ctgcttcccg agttaatagc taacactaat
      301 tttgacatat atttcacctc tcattttttt attatacaca aagacaagtt agcgtgctgt
      361 gattttgtta caaaaaagtt gattaatgat ttccccctat attattaata ataagagcaa
      421 attatctctt tatcttgttt gcggattaat attttgcgat aaataatttg gcaataagct
      481 agtaacaact ctaaaattgt aataactcaa atatttaaaa aataccgata atgcataaaa
      541 tttcaataaa ttgaatatta ttcgtctaaa tttgtcggta aattttcatt ttctgtcaaa
      601 attgcgataa ttccggtgtt taaactacca ttcttcgtcc ttttttatta taataataaa
      661 acgagggagg ttgattatga gttttataga tgaattcctg atggggttta caacgagtgc
      721 acataaatac tttggcgctc attttaatcc cgcgaaaggt aaaaagaaaa aaagtgtaac
      781 atttcgtctc tatgccccag cggcaagtga tgtaagtgtg attggggatt ttaacggttg
      841 ggatccaaat gtccacaaga tggagaaggt cgattaccgt ggtatttggg aaattactat
      901 tcctgaatta gaaaattacc aaaactataa attccgattc cggaatgccc atggtcatta
      961 tgtagataaa gctgacccat atgctttctt tagtgaaaaa gcaccaggta ctgcttcacg
     1021 gctatttgac tatgaaggtt ttatttggca cgacgaagat tggatgaaaa gtagatgtag
     1081 aggtcatgat aagccaatta gtatttacga gatccattta ggcagctgga aaggaaaaga
     1141 aaatgggcgg attttatctt acgaagaaat cgctgattac ttaattcctt atatgcgtga
     1201 taatggcttt actcatgtcg aaatcatgcc gattacacaa tatccatttg acgggtcatg
     1261 gggttatcag gctactggtt tctttagtgt tgatagtcgt tatggtaacc caatgcaact
     1321 tatgtcattt gtcgaccgta tgcaccaagc aggttttggc gttattttag actttgttgc
     1381 tgttcacttt gccattgacc attatggttt aattgaattt gatgggacaa gaatgtatga
     1441 atatcaagac agtgagcata catttagtca atggggttcc ccgcaatttg atttagggcg
     1501 cgatccagtt cgtagcttct taattagtgc gattttctat ttcttagaat acttccattt
     1561 cgacggaatt agaattgacg ctgtcagtaa cattgtctac tggcacggta atagtgaccg
     1621 cggagtaaat cacggcggtg ttgaattctt aaaacgactc aacggtaaag ttcattacca
     1681 tttccctggt gttatgatga ttgctgaaga ctcaacagcg tttgacggta caacgcggga
     1741 cgttgagtgg ggtggtttag gctttgacta taaatgggat atggggtgga tgaatgacac
     1801 cttacgctat tatagtaaag acccaattta taagaaatat gaccataacc tctttacatt
     1861 ctcgatgcat tatttcttt
//
LOCUS       DAOSEX010000181         1873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_62656_length_1873_cov_4.638064, whole genome shotgun sequence.
ACCESSION   DAOSEX010000181 DAOSEX010000000
VERSION     DAOSEX010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62656_length_1873_cov_4.638064"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1364
                     /locus_tag="PK612_03660"
     CDS             402..1364
                     /locus_tag="PK612_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95236.1"
                     /translation="MKLYVLENDLLKVTFLDVGASIYSFSVKALDNRNIVLTTKDKNI
                     YKEPPKNAYFGATVGRVGGRIANGEFIIDGKKYQAAQNEKVTNSLHGGFESYAAKLFE
                     VVEEKEDYITFRYVSRDGEGGYPGELELYVKYTLLDNCLALDYEARTTKKTLVNIINH
                     SYFNLNGKGDILKHKVSADVEAVFALDDKQINLLPLKVRKGDTFDLSTGKKLEKMVFA
                     KEVNVPPMGGVDHLLVTPKRKLTLEGEDLRLIVLSDYPAVQIYSTNYPPEVTLLEGTK
                     VIKYGGLAIEPADIVCGQNGEFKNLELCPGEVYKRRIKYIVELL"
     gene            complement(1356..>1873)
                     /locus_tag="PK612_03665"
     CDS             complement(1356..>1873)
                     /locus_tag="PK612_03665"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="catalyzes the formation of alpha-D-galactose
                     1-phosphate from D-galactose in galactose metabolism;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOH95237.1"
                     /translation="RRGLADSKYNERWNETMGALKTLKNHFKINNLTELKSSDLPQIE
                     KLLDPLLFRRVRHIITEQERTLESAMLLKEGKIEEFAKLLTASHNSLRDDYEVTGLEL
                     DTIVLLLLETGAIGARMTGAGFGGCAIAIVPKEKVEKQAPLVIDLYKKVIGYAPSFFA
                     SDSSDGTHELK"
BASE COUNT          601 a          321 c          371 g          580 t
ORIGIN      
        1 ggaagactaa tggcgacacg gcaactggtt taggtggcag tggtggtgga ccagatgcgt
       61 ggtggtttga agctggacac ttcccaaata atgctgacga acaatactat gttaccgctg
      121 aaggtctctt tagaactgat ccaacacctg ctccataatt aaacaataaa gtttagacat
      181 aaataatact cagataattt cattattatc tctatctcct cgcacacacc gtgcgaggag
      241 accttgttta caaaatgctc gtaattaaga atttggtcct agttatatca ttaataggac
      301 cttttctatt atttagtggc aaaatcagca tttattactg caaaacatac ttattttatg
      361 tataattaaa atgtatttat acaatggaaa aggataattt tatgaaatta tatgttttag
      421 aaaatgactt gcttaaagta acttttttag atgtcggtgc tagtatttat tcctttagtg
      481 ttaaggcgtt agataaccgc aatattgtct taacaacaaa agacaaaaac atttataaag
      541 aaccaccgaa gaatgcttat tttggcgcta ctgttgggcg ggttggtggc aggattgcta
      601 acggtgaatt tataattgat ggcaagaagt atcaagccgc gcaaaatgaa aaagtaacta
      661 attcactaca cggcggattt gaaagttatg ccgctaaact ttttgaagta gtggaggaaa
      721 aggaagatta tattactttc cgttatgtaa gtagggacgg tgaagggggt tatcctggtg
      781 aacttgaact atatgttaaa tacacattac tagataactg tttagcgctt gattacgaag
      841 cacggacaac aaagaaaaca ttagtgaaca ttattaatca tagttacttt aatttaaatg
      901 gtaaaggtga cattttaaaa cataaggtta gcgctgatgt tgaagcagta tttgcgcttg
      961 acgataaaca aattaactta ctgcctctta aagtaagaaa aggcgacact tttgacttaa
     1021 gcactggtaa aaagttagaa aaaatggtgt ttgccaaaga agtaaatgta ccaccgatgg
     1081 gcggggttga ccatttactt gttactccaa agcggaaatt aacgcttgaa ggtgaagact
     1141 tacgcttaat agttcttagt gattatccag cagtgcaaat ttatagtaca aactatccgc
     1201 cggaagtaac actacttgaa ggcacaaaag ttataaaata tggcgggtta gcaattgaac
     1261 cagcagatat tgtctgcggg caaaatggtg aatttaaaaa cctggaacta tgtccaggtg
     1321 aagtttataa gcgccgtatt aaatatattg ttgaattact ttaattcatg agtgccatca
     1381 ctactatcac tagcgaagaa tgacggtgca taacctataa ctttcttata taaatcaatg
     1441 acaagtggag cttgcttctc cactttttct tttggcacaa tcgcaattgc gcatccgcca
     1501 aagccggcac cagtcatcct ggctccgatt gctcctgttt ctaaaaggag aagaacaatg
     1561 gtatcaagtt caagccctgt tacttcgtaa tcatcgcgta acgaattatg tgaagcggtt
     1621 aaaagcttgg caaattcttc aattttcccc tctttcaaaa gcatagcgct ttctagtgtc
     1681 cgttcttgtt cggtaataat atggcgaact ctgcgaaata gaagcgggtc aagcagtttt
     1741 tcaatttgtg gtaaatcact gctcttaagt tctgttaaat tatttatttt aaaatgattc
     1801 tttaatgttt taagtgctcc cattgtttca ttccagcgtt cattgtattt agaatccgct
     1861 aaaccgcgcc gct
//
LOCUS       DAOSEX010000182         1864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_63154_length_1864_cov_3.186844, whole genome shotgun sequence.
ACCESSION   DAOSEX010000182 DAOSEX010000000
VERSION     DAOSEX010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63154_length_1864_cov_3.186844"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..1127)
                     /gene="tpiA"
                     /locus_tag="PK612_03670"
     CDS             complement(384..1127)
                     /gene="tpiA"
                     /locus_tag="PK612_03670"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOH95238.1"
                     /translation="MRKKLLVGNWKMNKTMAETAVFAHASFELLKNAKAKNIDIGVTP
                     PYLSLQTLLNINPKLIIGAQNVHHLEKGAYTGEISIPMLKELGVSWSLVGHSERRTYF
                     NETNESCNLKVLALLENNMTPLYCVGETLQEFEKGVTKEVIKAQIEVGLKGVKDVKEV
                     VIAYEPVWSIGTGKNASKEIAEDICAYIRELLRAKYGGDADLVRILYGGSVKPNNIKE
                     YMAMPNIDGALVGGASLSVESYAELINNM"
     gene            complement(1129..>1864)
                     /gene="pgk"
                     /locus_tag="PK612_03675"
     CDS             complement(1129..>1864)
                     /gene="pgk"
                     /locus_tag="PK612_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOH95239.1"
                     /translation="ASTVGVPELLKARGKEVALGFLVEKEVEALGRVVHVAKKDRPYI
                     AVIGGFKVSDKIKVIETLLQKCDKVLIGGAMAYTFQVAQGHNIGISPFEPEQVDFAKK
                     CLDTAGEKILLPVDAVATDAFQDWTKKITTTDSNIPDGFEGMDIGPKTQKLFADELSK
                     AKMIFWNGPMGVFEQKDFEAGTRAVCEAVSKLKGAFTVIGGGDSAAAVAQFGYKDYFS
                     HVSTGGGASLEMIENDGHLPGIDVIG"
BASE COUNT          537 a          409 c          294 g          624 t
ORIGIN      
        1 ctctgtgatt gatttcaaat ataaaatggt cgtttgccat gaattctttc ttgggtatac
       61 ttgcttttaa tcctagcgca agtaacatga ctaaacttac gtaagcaatt ttattagtat
      121 taatccgtct gcacatagtt tctcccttac gctaaagaat agcgtcacca tttgatcgaa
      181 taatcattga ccactacata taaacaagtg acttaaaaaa tataatgtaa ttttatcatg
      241 agttttatat acgctatcct ctttttataa atcacatttt catcatcttt aactactgta
      301 tattattttt cgacctcaaa agtcgctatt taaaaaggga accattttct tggtcccctg
      361 taatttgctt tatgtagata acactacata ttattaataa gttcagcgta actttcaaca
      421 ctaagcgagg caccgccaac gagggcacca tcaatatttg gcatcgccat atattcttta
      481 atgttgttag gtttaactga gccgccgtaa agaattcgta ctaaatcagc gtcaccgccg
      541 tatttagcgc gtaataattc acggatataa gcgcagatgt cttctgcaat ttctttactt
      601 gcatttttac cagtgccaat tgaccacact ggttcataag caatcacgac ttctttaacg
      661 tctttaacac ctttaagccc cacttcaatt tgtgctttaa ttacttcttt tgtcacacct
      721 ttttcaaatt cttgtaatgt ttcaccaaca cagtatagtg gtgtcatatt attttcaagt
      781 aaagcgagga cttttaagtt acagctttca tttgtttcgt taaagtacgt ccttctttca
      841 ctgtgaccga caagtgacca actaacacct agttctttta gcattggaat actaatttca
      901 ccagtgtaag cccctttttc aaggtgatga acattttgcg caccaataat gagctttgga
      961 ttaatattta ataaggtttg cagtgataaa taaggtgggg tgaccccaat atcaatattt
     1021 ttagctttgg catttttaag taattcaaag gaagcatgag cgaaaactgc tgtttccgcc
     1081 attgttttat tcatcttcca gttgccaaca agtaatttct tccgcatatt atccaatcac
     1141 atcaatgcct ggtaaatgac catcattttc aatcatttct agtgatgctc cgccacccgt
     1201 gcttacatgg ctaaagtaat ccttataacc aaattgcgcg acggcggctg cactatcacc
     1261 accaccaatg acagtgaatg ctccttttaa tttactcaca gcttcgcaca cagcgcgtgt
     1321 accagcttca aaatcttttt gttcaaagac acccattggt ccgttccaga aaatcatttt
     1381 ggctttgctt aattcatccg caaataattt ttgtgttttt ggcccaatat ccatcccttc
     1441 aaaaccatca ggaatattcg aatccgttgt cgttattttc tttgtccagt cttggaaggc
     1501 atcagtggca acagcgtcaa ctggtaataa aatcttctca ccagcagtgt ctaaacattt
     1561 ctttgcaaag tcgacttgtt caggttcaaa cggtgaaatc ccgatattat gaccttgagc
     1621 gacttggaaa gtgtaagcca ttgccccacc aattaatact ttgtcacatt tttgtaataa
     1681 agtttcaata actttaattt tgtcactcac tttaaagcca ccaataacgg caatataagg
     1741 ccggtctttt ttagcgacat gaacaacacg gccaagtgct tcgacttctt tttcgactaa
     1801 aaagccaagc gcaacttctt taccgcgggc ttttaagagt tcaggaacac cgacagtcga
     1861 agcg
//
LOCUS       DAOSEX010000183         1859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_63418_length_1859_cov_2.924612, whole genome shotgun sequence.
ACCESSION   DAOSEX010000183 DAOSEX010000000
VERSION     DAOSEX010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63418_length_1859_cov_2.924612"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PK612_03680"
     CDS             complement(<1..834)
                     /locus_tag="PK612_03680"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH95240.1"
                     /translation="MAKKYDLIIVGGGSAGLGAAVAAYDAGIHNILLIEKEATFGGIL
                     NQCVHDGFGVHLFKKTLTGPEYAHYLVEMLKERPFDILLSSNVTDISDKQEVTVFVAG
                     QGVMLFKTKAIILTTGSYERSAGAINLPGDRGAGIMNAGQAQYFLNLKGYEFAKDVLI
                     VGSGDIGLIMARRLSLLGIKVHAVSEIMPHSSGLKRNIVQCLDDFNIPLYLSHKVTDV
                     RGKPRIEEVDIAELDDKFEIIPGTTKTFKVDTLLLAVGLIPYVALSDNLGIPTSKSRG
                     PV"
     gene            complement(818..>1859)
                     /locus_tag="PK612_03685"
     CDS             complement(818..>1859)
                     /locus_tag="PK612_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH95241.1"
                     /translation="DHIVGGLFAPTAGIVNPFELTAHAIENAIENGMTLKLNEEVVAI
                     KKLEDGFKVTTKNGDYFSKVVINAAGVFADDIINMVSKADFEITPRKGSYFVLDKLTV
                     PLVNTVLFPLPNIHSKGILVVPTTAGNTLIGPTSEQIMSKVDVGTDYASLEFIRKNAN
                     RLLKDIPFHKTIRTFTGLRATPSTGDFIIEHDKYVKGLINVAGIESPGLASAPAISLY
                     VVNELVKDLLPLKVKANFNPRVRPLIRLQRLSTKEIQAKVKENKDFATLVCNCEKVTK
                     AEIIDVLSRALPVNSVKAMKKRLRTGFGLCQGGFCTAKVIAIMSEYYGIPVDEIAYNE
                     KESYVVKHGKKV"
BASE COUNT          561 a          415 c          302 g          581 t
ORIGIN      
        1 tactggcccc cgtgatttac tcgtgggaat cccaagatta tcactaagcg caacgtaagg
       61 aataagcccg accgctagaa gcagtgtgtc aactttaaat gttttagttg tccctggaat
      121 gatttcaaac ttatcatcaa gctcagcgat atcaacttcc tctatccgtg gcttaccgcg
      181 tacatcagtg actttatgtg ataaatatag cgggatatta aagtcatcta aacattggac
      241 aatattacgt tttaagccgc ttgaatgggg cataatttca ctaacagcgt gcactttaat
      301 cccgagtaaa ctaagccgac gagccataat taagccaata tcaccagagc caacgattaa
      361 gacgtctttg gcaaattcat aacctttgag atttaggaag tattgagctt gaccagcgtt
      421 catgattcca gcaccgcggt cgcctggtaa attaattgct cccgcacttc gttcgtatga
      481 ccctgttgtt aaaataatcg cttttgtttt aaaaagcata acaccttgac cagcgacaaa
      541 tactgtcact tcttgcttgt cactaatatc agtgacatta ctactaagta aaatatcaaa
      601 aggtcgctct tttaacattt cgacaagata atgggcatat tctggtcctg ttaatgtttt
      661 cttaaataaa tgtacaccga acccatcatg cacacattga tttaaaatac cgccaaaagt
      721 tgcttctttt tcaattagta aaatattatg aattcctgcg tcataagccg caacagcagc
      781 gccaagtcca gcactaccgc caccaacaat aattagatca tacttttttg ccatgtttca
      841 ccacgtaact ttctttttcg ttatacgcta tttcatcaac gggaatcccg taatattcac
      901 tcataatcgc aataactttt gccgtacaaa atccgccttg acataaacca aatcctgttc
      961 gtaaacgctt tttcatcgct ttaacgctgt taactggtaa agcgcggctc agcacatcaa
     1021 taatctcggc ttttgtcacc ttttcacaat tacagacaag tgtcgcaaag tctttatttt
     1081 ctttaacttt tgcttgaatt tctttagtgc ttaagcgttg taaacgaatc agcggtctaa
     1141 cgcgcggatt aaaattagct tttactttaa gcggtaataa atctttcact aattcattaa
     1201 cgacataaag cgaaatcgct ggggcacttg ctaaaccagg actttcaata ccagcaacat
     1261 taattaaacc tttaacatat ttatcatgtt caataataaa gtcaccagtc gatggtgttg
     1321 cccgtaaccc ggtgaaagtt cgaattgttt tatggaacgg aatatctttt aataaccgat
     1381 tagcgttttt acggataaat tcaagtgaag cataatccgt cccaacatca acttttgaca
     1441 taatttgttc acttgtcggt ccaatcagtg tattaccagc ggttgttggg acaactaaaa
     1501 tccctttact atgaatatta ggcagcggga ataatactgt atttacaagt ggaaccgtta
     1561 atttatcaag gacaaaataa ctaccctttc gcggagtaat ttcaaaatca gccttactta
     1621 ccatattaat gatatcatcg gcaaatacac ccgctgcatt aatcacaact ttactaaaat
     1681 aatcaccatt cttcgtcgta actttaaagc cgtcttcaag cttttttatc gctactacct
     1741 cttcattaag ctttaaagtc atcccgtttt caattgcatt ttcaatcgcg tgtgccgtta
     1801 gttcaaaagg gtttactatc cctgctgtcg gtgcaaatag gccgccgaca atatgatca
//
LOCUS       DAOSEX010000184         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_63595_length_1855_cov_3.475000, whole genome shotgun sequence.
ACCESSION   DAOSEX010000184 DAOSEX010000000
VERSION     DAOSEX010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63595_length_1855_cov_3.475000"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PK612_03690"
     CDS             <1..1333
                     /locus_tag="PK612_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH95242.1"
                     /translation="DIDDPDCIDVLAAFQEYYLENYGEEVTIEYNTFSTNEDMYNIVK
                     LNGAKYDLIAPSDYMIQRMIREDMLEKYDLDAEGNYKYVPNYNEYASPYLIDLFKEDL
                     GIYEYAPGYMWGTMGFLYNSEFNDTINDDVQSWMMLWDKKYSKKTSIKDSMREGYLMG
                     LFKYYEDELNDLAAQREAGLISDDYYNRELSDLLNDVDPDTIKEVIHVMNELKNNIYG
                     LEVDQGKIDMITGKIAANTAWSGDAVYAIEEDPKDVLRYAIPKEGSNIWFDGWIMPKG
                     ANRVVAQRFVNFVSSPEMAIANMYYIGYTSFIAGDEVLEYVLDNDAVYEAAEAEETVE
                     LDFTYFFSDTIENPSDALITIDASYVGHTLTATYPTEEEIVRSVVMRDFGDSNKLVID
                     MWANFKATDIQNWMIIFSVIIVLAVGSAVTLNQVQKHKAARRKRNLYKDKQ"
     gene            1330..>1855
                     /locus_tag="PK612_03695"
     CDS             1330..>1855
                     /locus_tag="PK612_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOH95243.1"
                     /translation="MKKRLAVIGGGVSGVTLARLVQGSFNVTIFERESSLLQKLLRTG
                     NGRANIYNRNIHSFAYNDEHFLSEHLPHIVKTLDDLFSAFNIVTYTDTEGRVYPYSRS
                     AKALRALLIKDLKAEVKLEHNVEEIVKVSDKYTVDGEEFDAVALTMGSSAGLFKYPLD
                     NGNAALLSALDLETT"
BASE COUNT          601 a          290 c          416 g          548 t
ORIGIN      
        1 agatattgat gatccagatt gtattgatgt tttagcggcc tttcaagaat attatttaga
       61 aaattatggc gaagaagtca caattgagta caatacattt tcaacgaatg aagatatgta
      121 taatatcgtc aaactcaatg gcgcaaagta tgacttaatt gccccaagtg actatatgat
      181 tcaacggatg attcgtgaag atatgctcga aaagtatgat cttgacgcag aaggtaatta
      241 taaatatgtt cctaattata atgaatatgc aagtccatat ttaattgatc tttttaaaga
      301 agatttagga atttatgaat acgccccagg gtatatgtgg ggaacaatgg gttttcttta
      361 taacagtgaa tttaatgaca cgattaatga cgatgtccaa agttggatga tgttatggga
      421 taaaaaatat agtaagaaaa cttcgatcaa agatagtatg cgcgaaggtt atttaatggg
      481 actttttaaa tattacgaag atgaacttaa tgatttagcc gcgcagaggg aagcagggct
      541 tattagtgat gattattaca atcgcgagtt aagcgaccta cttaatgatg ttgaccctga
      601 tacgattaaa gaagtgattc acgtgatgaa tgaacttaaa aataatattt atgggttaga
      661 agttgaccaa ggtaagattg atatgattac tggaaaaatc gctgcgaaca ccgcgtggag
      721 cggggacgcg gtgtatgcca ttgaagaaga cccaaaggat gtgcttcgtt acgctattcc
      781 aaaagaaggt agtaatattt ggtttgatgg ctggattatg cccaagggcg ctaatcgtgt
      841 cgtcgcacaa cgctttgtga actttgtgag tagtccggaa atggcaatcg ctaatatgta
      901 ttacattggt tacactagtt ttattgcggg ggatgaagta cttgaatatg tgcttgataa
      961 cgatgcagtg tatgaggcag cagaagcaga agaaacagtt gagcttgact ttacatattt
     1021 ctttagtgat acaatcgaaa atcctagtga cgccttaatt acgattgatg cgagttacgt
     1081 tggtcataca ttaaccgcga cttatccgac ggaagaagaa atcgtccgca gtgtcgtcat
     1141 gcgtgacttt ggtgatagta ataagctcgt cattgatatg tgggcaaact ttaaagcgac
     1201 ggacattcaa aactggatga ttatctttag tgttatcatc gttttagcag ttggtagtgc
     1261 cgtcaccctt aaccaagtcc aaaaacataa ggcagcgcgg cgtaagcgca atttatataa
     1321 agataaacaa tgaaaaaaag attagcagtc atcggtggtg gagtaagcgg agttacctta
     1381 gcgcgacttg tgcaaggttc ctttaatgtc accatctttg agcgtgaatc tagtctctta
     1441 caaaaacttt tacgcacggg taatggacgc gcgaacattt ataatcgcaa catacattcc
     1501 tttgcttata atgatgaaca ctttttaagt gaacacttac cacatatcgt taaaacgctt
     1561 gatgacttat ttagtgcttt taatattgtc acttacacgg atacagaagg aagagtatat
     1621 ccctatagca gaagtgctaa agcactccgc gcattactca ttaaagattt aaaggcagaa
     1681 gttaagttag aacataatgt cgaagaaatc gttaaagtta gtgataaata taccgttgat
     1741 ggtgaagaat ttgacgctgt tgcgttaaca atgggcagta gcgcgggact ttttaagtat
     1801 ccgcttgata acggtaacgc ggcgctttta agtgctctag atttagaaac aactg
//
LOCUS       DAOSEX010000185         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_64317_length_1841_cov_2.870101, whole genome shotgun sequence.
ACCESSION   DAOSEX010000185 DAOSEX010000000
VERSION     DAOSEX010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64317_length_1841_cov_2.870101"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      183..262
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            450..1673
                     /locus_tag="PK612_03700"
     CDS             450..1673
                     /locus_tag="PK612_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95244.1"
                     /translation="MAEEINLSEHPLLEVADDIIREEKRRKRTKKVVAVSAFIIIVFI
                     IISYLGYAIGYYIANATSLSLLSKDPEARFAQVGFRLSEPLTVTEQHKYNLQSDEGWV
                     GGEGYVYWTTQNVSPQTMSFVLEHEGYSNTNRLSPVTSGSFTKDEDPYLPLTLYEAPK
                     QNRAERHNLNLNVADPKDYFGFTVLFRLLIDEDRAGTIPLDNIAIYFNRDTKVHGQNE
                     LPKALRFGFQSGAKKDIISPGRTLLEENDYIGSIAVGGRLDLDDPRAPASLTGFYDYT
                     FGLDGAKYGESGEQIYEIAYGEFEQPLTNENWGEPLAEAEEKLEHGDVFHAVTYKGVR
                     PLINVVPQRQYYTSFQKYMLDGDKGEPLCYTNNLGVAEMNIKFWLEGWDLATTNKVVN
                     KSFGMQLHFVAKRAE"
BASE COUNT          624 a          308 c          386 g          523 t
ORIGIN      
        1 agcgcgcaaa caagcgcttg aagaactccg taaagagtta gcaaaacaaa acgaacccgc
       61 tcctgaaacg aaacctgaag aataattaat cacaaaatac ttaaaattgc cgtagaaaat
      121 acggcttttt aatacttaaa ttagatagtt cttgacactt ttatatgtaa aacatataat
      181 aactacagaa aaggcaaact tagagaaatc taaggacgca aaactatagg gcctaaaagt
      241 gtgatggtag ccagttgcct cgaagaaata aagatgaaag ttaatatttc tggcaaagag
      301 tggttagcat catttttatt taatcgccac tatcaaatta taaaaatagg atagtgtgca
      361 cggacattcg tgttaaaata ataaagaatg ttcatatatc actaaaacat cgtagattgt
      421 tttagaggta taggaaaacg gagaatattg tggcagaaga aattaattta agtgaacatc
      481 cacttcttga agttgctgac gacattattc gtgaagaaaa gcggcggaaa cgcactaaaa
      541 aagttgtcgc tgtttcagcg ttcatcatta ttgtttttat cattatttca tacttagggt
      601 atgcaattgg ttactatatt gcaaacgcaa cctcgctttc cttattgtca aaagacccag
      661 aagctcgctt tgcgcaagtt gggtttagac ttagtgaacc gctaacagtt acagaacaac
      721 ataaatacaa tttacagtct gatgaaggtt gggttggtgg tgaaggttat gtgtattgga
      781 caacgcaaaa cgtgtcgcca cagacgatga gttttgtgct tgagcatgag ggctattcaa
      841 atacaaatag acttagccca gttacttctg gttcttttac gaaagatgaa gacccatatt
      901 taccacttac gctttatgaa gcaccgaagc aaaaccgtgc agaacgccac aatttgaact
      961 taaatgtagc agatccaaaa gattattttg ggtttacagt attatttaga cttttaattg
     1021 acgaagatcg agccggaaca atcccgcttg ataatatagc catttacttc aatagagaca
     1081 cgaaggtaca cggtcaaaat gaattaccaa aagcattgcg ttttggtttc caaagtggag
     1141 ctaaaaaaga catcattagt ccagggcgca ctttattaga agaaaatgat tatataggtt
     1201 caattgctgt tggtggcaga cttgacttag atgacccgcg tgctccagcg tcacttacag
     1261 gattttatga ttacacattt ggtcttgatg gggcgaagta tggcgaaagt ggtgaacaaa
     1321 tttatgaaat agcttatggc gaatttgaac agccactcac aaatgaaaac tggggtgaac
     1381 cacttgcgga agcagaagaa aagttagaac atggtgatgt tttccatgcg gtgacttaca
     1441 agggagtccg tccgcttatt aacgttgtac cacagcgcca atattacaca agtttccaaa
     1501 aatatatgct tgatggtgat aaaggtgaac cgctttgcta cacaaataac cttggtgtgg
     1561 cagaaatgaa tattaaattt tggttagaag gctgggactt agcgaccaca aataaagttg
     1621 taaacaagtc ttttggaatg caattacact ttgtcgctaa aagggctgaa taagaagagg
     1681 aaatggtgat attatgaacg cattttttag ttatatccga gacacatttg aatcattctg
     1741 ggctaacttt gtcgaatggt tagccgctgt atttgttgaa ccatggaaac tagtgtccga
     1801 tgactttggt acttatcgta caatcattga aatgtacatg g
//
LOCUS       DAOSEX010000186         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_64909_length_1830_cov_2.430423, whole genome shotgun sequence.
ACCESSION   DAOSEX010000186 DAOSEX010000000
VERSION     DAOSEX010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64909_length_1830_cov_2.430423"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PK612_03705"
     CDS             <1..595
                     /locus_tag="PK612_03705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH95245.1"
                     /translation="LFEELGIKGDENTPLFSMVTRLTWQKGIDLVIPAIIEVLEKGGN
                     VVVVGSGEHGYEQALEELRARYPDRMAIYIGYNNPLAHRVYAASDFFFMPSLFEPCGI
                     GQMIAQRYGTLPIVRTTGGLKDTVIGYDGTNEDVADGFVFDNYDEYWMKLTTNYALEQ
                     YKNQKVFKKLRRNAMEKNNDWSEAGKKYLALYKSIVK"
     gene            770..>1830
                     /gene="leuS"
                     /locus_tag="PK612_03710"
     CDS             770..>1830
                     /gene="leuS"
                     /locus_tag="PK612_03710"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOH95246.1"
                     /translation="MAYDFRAAEKKWQKYWEENNTFYTDMYDFSKPKYYVLDMFPYPS
                     GDGLHVGHVEGYTATDIVARFKRMQGYNVLHPIGFDAFGLPAEQYAIRTNNHPAIFTN
                     ENIANFRRQLKSLGFSYDFSKELSTSEPKYYKWTQWIFTELFKSDLAYVDNIPVNFCP
                     ELGTVLANEEVIDGKSEVGGFPVIRKPMRQWLLRITEYAERLLSDLDGLDWPASTLDM
                     QRNWIGKSEGTTITFKVANTNYEFDVFTTRVDTLYGATYACLAPEHPLVLKVVSDTQK
                     EEVKRYIESASHKSDLERTELNKDKSGVYLGVNAINPLNNKEIPLYVADYVLGSYGEG
                     AVMAVPAHDERDYEFAKRHG"
BASE COUNT          556 a          319 c          420 g          535 t
ORIGIN      
        1 actctttgaa gaactaggca ttaaaggtga tgaaaacaca ccgctatttt caatggtgac
       61 gcgattaacc tggcaaaaag gcattgattt agttattccc gcaattattg aagtattaga
      121 aaaaggtggc aatgttgtcg ttgtcggtag cggggaacat gggtatgaac aagcgcttga
      181 agaactgcgc gctcgctatc cagaccgtat ggcaatttat atcggttaca ataatccact
      241 tgcgcaccgc gtttacgcag cgagtgattt cttcttcatg ccaagtttat ttgagccgtg
      301 cggtattggg caaatgattg cccagcgcta cgggacgctg ccaattgtgc gtactaccgg
      361 tggtttaaaa gatacggtaa ttgggtatga tggcactaat gaagatgtcg ctgatggttt
      421 tgtctttgat aattatgacg aatactggat gaaactcacg acgaattatg cgcttgaaca
      481 atacaaaaat caaaaggtct ttaaaaagct tcgccgcaat gcgatggaga aaaataatga
      541 ctggtctgaa gctggtaaaa aatacctagc gctctataaa agtatcgtta aataattatt
      601 gtgatataat tttatcgcga gtgaagaata aataacttat attaagttat gtctcacctt
      661 cagttaactc gctgtgccct agcattaata ggaaatgaag ttataaaata aggtggtacc
      721 gcggactttc cgccctttgg taacaccttt tatttttgga ggtaacatta tggcttatga
      781 ttttcgtgcc gcagaaaaga agtggcaaaa atattgggaa gaaaataaca cattttatac
      841 agatatgtat gactttagta aaccaaagta ttatgtgtta gatatgtttc cctatccttc
      901 aggcgacgga ctgcatgttg gtcacgttga agggtatacc gcgactgata tcgtcgctcg
      961 ctttaaacgg atgcaaggct ataatgtctt acacccgatt ggatttgacg cctttggttt
     1021 accagcggaa caatatgcga ttcgcacgaa taatcatccg gctattttta ctaacgaaaa
     1081 tatcgctaat tttagacggc aattaaagtc gcttggcttt agttatgatt ttagtaaaga
     1141 actttctact agtgaaccaa agtattataa atggacacag tggattttca ctgaactttt
     1201 taaaagcgac ttagcctatg tcgataatat ccctgttaac ttttgtcctg aactaggaac
     1261 agtactcgca aatgaagaag taattgacgg taaaagtgaa gttggcggat tcccggtgat
     1321 tcgtaaaccg atgcgccagt ggttattacg cattacggag tacgcggagc ggttattaag
     1381 tgatttagac ggtttagatt ggcccgcttc tacgcttgat atgcaacgta actggattgg
     1441 gaagagcgaa ggcacaacga ttacttttaa agtagcgaat acaaattacg agtttgatgt
     1501 ctttacaacg cgtgttgata cgctttatgg ggcgacttat gcatgtttag cgcctgaaca
     1561 tccgcttgtg ttaaaagttg ttagtgacac gcaaaaagaa gaagtaaagc gttatattga
     1621 aagcgctagt cataagagcg acttagaacg gacagaactt aataaagata aatcaggggt
     1681 gtatttagga gttaacgcta ttaatccgct taataacaaa gaaatcccgc tttatgttgc
     1741 tgattatgtt ttagggtcat atggtgaagg cgcagtaatg gcggttccag cgcatgacga
     1801 acgtgattac gaatttgcta agcgccacgg
//
LOCUS       DAOSEX010000187         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_65911_length_1811_cov_3.075171, whole genome shotgun sequence.
ACCESSION   DAOSEX010000187 DAOSEX010000000
VERSION     DAOSEX010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65911_length_1811_cov_3.075171"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="eno"
                     /locus_tag="PK612_03715"
     CDS             complement(<1..924)
                     /gene="eno"
                     /locus_tag="PK612_03715"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006838607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOH95247.1"
                     /translation="MPTIIDVYAREVIDSRGNPTIEVDVTTESGAFGRAIVPSGASTG
                     EREALELRDGDKSRYLGKGVLTAVENVNKIIAPEIIGMDADDQVGIDKVLLELDGTPF
                     KKKLGANALLGVSLAAARAAADYFGVSLYNYIGGVNAKMLPVPMMNVINGGAHADSSV
                     DFQEYMIMPVGAPSLREALRWGAEVFHNLKKVLKAKGHITAVGDEGGFAPNLSSNEEP
                     LQVIVEAIKKAGYVPGKDFVLALDVAASEFFDEKTKKYVLKKSGGAVKTSEEMVTWYE
                     ELCEKYPIISIEDGLAEGDWDGWKLLTDRLSH"
     gene            1081..1485
                     /locus_tag="PK612_03720"
     CDS             1081..1485
                     /locus_tag="PK612_03720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOH95248.1"
                     /translation="MEQKEIKNVVAALIFYDGKLFAASRSEKDERYGKYEFPGGKIEL
                     EENPSSAIVREIKEELMMDISVKDLFMHVSHEYPWFKLEMDVFICEVTNPQFTLLVHK
                     DGGFFTKEEILKLPFLGADLQVLAEISKRLDF"
     gene            complement(1482..>1811)
                     /locus_tag="PK612_03725"
     CDS             complement(1482..>1811)
                     /locus_tag="PK612_03725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOH95249.1"
                     /translation="ARFMEGSPYTEKNAAIIKMLEGHSDKSAKFICAIALVGLDSFPQ
                     VFIGEVKGTIINEARGEEGFGYDPIFFHPNSQKTFAEMTLEEKDEISHRGRATALLFE
                     YLETHYF"
BASE COUNT          537 a          376 c          312 g          586 t
ORIGIN      
        1 atggcttaaa cggtcagtta aaagtttcca gccatcccag tcgccttctg ctaaaccgtc
       61 ttcaatcgaa ataattgggt acttttcaca taattcttcg taccatgtaa ccatttcttc
      121 acttgtttta acggctccgc ctgatttctt aagcacatat ttctttgttt tttcatcaaa
      181 gaattcactc gccgcgacgt caagcgctag aacaaagtct ttacctggaa cgtatcctgc
      241 ttttttaata gcttcaacga ttacttgtaa aggttcttcg ttactactta agtttggtgc
      301 aaagccgcct tcatccccaa ctgcggtaat atgacctttg gcctttaaaa ctttctttaa
      361 gttatggaaa acttcagcgc cccagcggag tgcttcgcgt aagcttggtg ccccaacagg
      421 cataatcata tattcttgga aatcaacact gctatcagca tgtgcgccac cgttaataac
      481 attcatcatt gggactggta acattttggc attaacacca ccaatgtagt tatataaact
      541 tacaccgaaa taatctgcag cggcacgagc agcagctaaa ctaacaccaa gtaatgcgtt
      601 agcgcctaat ttctttttaa atggggtacc atcaagttct aataaaactt tatcaattcc
      661 cacttggtca tcggcatcca ttccgataat ttctggagca ataattttgt taacattttc
      721 aacagcggtt aacactcctt tgccgagata gcgactttta tcaccatcac gtaattctaa
      781 tgcttcgcgt tcacctgtac tagcgccact tggaacaatt gcacgtccaa aagcaccaga
      841 ttcagtcgta acatcaactt cgatcgttgg gttaccacgt gagtcgatta cttcacgagc
      901 atacacatca ataattgttg gcatgtattt ttcctcctct ttgtaaatag ccacatatat
      961 tataatattt tttctgcttg ctttgtagta aaaagaacta aagacatcgt tttatcagta
     1021 aatatatccc gatatcaatt tacagcgtaa aaatatgtta ctttgttata ctaataaagt
     1081 atggaacaaa aagaaattaa aaatgttgtg gcggccctta tcttctatga tggtaaactt
     1141 ttcgctgctt cacgaagtga aaaagatgaa cgctacggta aatatgaatt tcccggtgga
     1201 aaaattgaac tagaagaaaa tccaagttct gctattgtac gcgaaattaa agaagaatta
     1261 atgatggata taagtgtcaa agacttattt atgcatgtaa gtcatgagta tccatggttt
     1321 aaacttgaaa tggatgtttt tatttgtgaa gttactaacc cgcaatttac cttattagtg
     1381 cataaagatg gtggtttttt tacaaaagaa gaaatattaa aattgccctt cttaggggct
     1441 gatcttcaag tgcttgctga aattagtaag cgtttagact tttaaaaata atgcgtttct
     1501 aaatattcaa atagtaatgc cgtcgccctt ccgcgatggc taatttcatc tttttcttca
     1561 agcgtcattt cggcaaatgt tttttgactg tttggatgaa agaatatcgg gtcataacca
     1621 aatccttctt cgccacgcgc ttcgtttatt atagtgcctt taacttcgcc aataaacact
     1681 tgcgggaaac tgtcaaggcc gacaagggca atagcacata taaactttgc gcttttatca
     1741 ctgtggcctt caagcatttt aataattgca gcgtttttct cagtataagg tgagccttcc
     1801 ataaaccgcg c
//
LOCUS       DAOSEX010000188         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_65984_length_1810_cov_2.860399, whole genome shotgun sequence.
ACCESSION   DAOSEX010000188 DAOSEX010000000
VERSION     DAOSEX010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65984_length_1810_cov_2.860399"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PK612_03730"
     CDS             <1..749
                     /locus_tag="PK612_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HOH95250.1"
                     /translation="HHEIEEVKDGYNITMEVTNIGHIRGAEVIGIYTGKSRSLLYGPR
                     KELRAFKKVYLDPGEKQKINIFLPTESLKAYCPVKQEWVLENVNYNVYVGINVQTTDE
                     IFQILVDDANDLSYLKNYQKENEMYYRRADFNPSIKDFTTLFGGESLPLFFANRKRPF
                     TLANNISDTTDYWWGRTIYKIMAKTSRKLAGDDVVLQEMAVETIKELPFRSLSALSSG
                     EVSTNQRDAILLLVNGKPIRGIFKFLRKKK"
     gene            821..>1810
                     /locus_tag="PK612_03735"
     CDS             821..>1810
                     /locus_tag="PK612_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOH95251.1"
                     /translation="MSEEKVSTTQDLKVQKQTKRDRRYLINIIVILLITFGYTTYSLW
                     NELPAVINVFRGTEADYRMIIIIIVLIALKFFIEGIILFIFARLYTPTYKIHRGVANA
                     FIGQFYNDITPSATGGQFAQVKTFAQQGVPVSIGASIMVMHFILYQIVLVVFGGLLIL
                     LNLGEFARLGSVSIFKLNIPVWVFASFGFLANLVTIVGLFLLAYSRRVHHFVINVVVD
                     LLAKIKIVKRPEYRKNKLYTSTENFRIELRRLSSNVPASILIMVLFFLKFIVNYSVTY
                     FVALMLNPALYGGLSFWETISKTGFLGMITGFIPIPGAAGFAEYFYEIIFSPVF"
BASE COUNT          548 a          292 c          342 g          628 t
ORIGIN      
        1 gccatcatga aattgaagaa gtaaaagacg gttataacat cacgatggaa gtgacgaata
       61 tcggtcatat ccgcggcgca gaagttattg gtatttacac aggaaagagc cgtagtttgc
      121 tctacggtcc tcgtaaagaa ttacgcgctt ttaagaaggt gtatcttgat ccaggtgaaa
      181 aacaaaagat caatatattc ttacctactg agtcattaaa agcttattgt ccagtaaagc
      241 aagagtgggt gcttgaaaat gtaaattaca atgtttatgt cggaataaat gtacaaacca
      301 ctgatgagat cttccaaatc ttagtggatg acgctaatga tttaagctat ttaaaaaatt
      361 atcaaaaaga aaatgaaatg tattatcgtc gcgccgattt taatccatca attaaagatt
      421 ttacaacatt atttggtggt gaatctttac cattattttt cgctaacagg aagcgtccct
      481 tcacacttgc taataacatt agtgatacga ctgattactg gtggggacga acaatttata
      541 agattatggc caagacttca cgcaaactag cgggtgatga tgtagtactc caagagatgg
      601 ctgttgaaac gattaaagaa ttaccgttta gaagtctttc agccttaagt tctggagaag
      661 taagcactaa tcaacgagac gctattttac tattagtaaa tggcaaaccg attcgtggta
      721 ttttcaagtt tttacgaaaa aagaaataga aacatgtatg aatatacctt tcaagttgtc
      781 ttaactaagg acttaagtcc tttcatgcta taatttacct atgagcgaag agaaagttag
      841 cacaacccag gatttaaaag tacaaaagca aacgaagcgt gatcgtcgct atcttattaa
      901 tatcatcgta attttattaa ttacttttgg gtacactact tactcattat ggaatgaact
      961 cccagcggta ataaatgtgt tccgtggcac ggaagctgat taccgcatga ttataataat
     1021 tattgtttta attgctctaa agttttttat tgaaggcatt atcttattta tttttgctag
     1081 gctttatacc ccgacttata aaatacatcg cggtgtcgcg aatgctttta ttggtcaatt
     1141 ttataatgat attactccga gtgctacagg tggtcaattt gcgcaggtaa aaacttttgc
     1201 gcaacaagga gtgccagtat caattggagc ttccattatg gtgatgcact ttattttgta
     1261 tcaaattgtg cttgttgttt ttggtggatt acttattctt ttaaaccttg gagaatttgc
     1321 gcgacttggt tcggttagta tttttaaact taatattcca gtgtgggttt ttgcttcatt
     1381 tgggttttta gctaacttag ttacgatagt tgggctattt ttactagcgt actcgagacg
     1441 tgttcatcac tttgttatta atgttgtagt cgatctttta gcgaaaatta aaattgttaa
     1501 aagacctgaa tatcgtaaaa ataaacttta tacttcaact gagaacttca gaattgagtt
     1561 acggcgctta agttcaaacg tgccagcttc aattcttatt atggttctct ttttcttaaa
     1621 atttattgtt aactattcag ttacatattt tgtggcttta atgttaaatc cagcattgta
     1681 tggcggtctt tccttttggg aaacaatttc taagactggt ttcttaggaa tgattacagg
     1741 ctttattcca attcctggcg ctgctgggtt tgccgaatat ttttacgaaa tcatcttctc
     1801 ccctgttttt
//
LOCUS       DAOSEX010000189         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_66163_length_1807_cov_2.641553, whole genome shotgun sequence.
ACCESSION   DAOSEX010000189 DAOSEX010000000
VERSION     DAOSEX010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66163_length_1807_cov_2.641553"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PK612_03740"
     CDS             complement(<1..1019)
                     /locus_tag="PK612_03740"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOH95252.1"
                     /translation="MIRLNYDNVLATIDFEKYRSKLEKINEGINQKKGAGNDYLGWAD
                     WPLHYDKKEFNLIKETALMIRETFDTLVVTGIGGSYLGARAAIEAINGLLPQKGPEII
                     FLGHTFSPTLTAQTLEYLKTKNFAINVISKSGTTTETSIAFRLLKQLLIEKVGEANAN
                     KAIFATTDAKTGALRKLATQNGYTTFTLPSDIGGRYSVLTAVGLLPLAVAGIDIDDLM
                     AGALKARSESVDNLDHDLYKYAVIRDTLYRQNYKSELYVVYEPQYAMFNEWLKQLYGE
                     SEGKEKKGLFPTSVTFSTDLHSLGQFIQDGSPILFETTLFVENPNQDVVIPSEKENLD
                     ELNYLA"
     gene            complement(1012..1383)
                     /locus_tag="PK612_03745"
     CDS             complement(1012..1383)
                     /locus_tag="PK612_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOH95253.1"
                     /translation="MNIYELNEIVEGTITEIRPYGAIITFPENRTGLLHIKQISDAYI
                     SNINTYLQLDAQIKVRIIEIDEHNGFLKLSLKSVPYNERIIFERDKKATNINPAQTDF
                     SALEKALPLWIEKAIKEAQND"
     gene            complement(1386..>1807)
                     /locus_tag="PK612_03750"
     CDS             complement(1386..>1807)
                     /locus_tag="PK612_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOH95254.1"
                     /translation="FYFDPQTKLPVWPKLAINYTAKTQFYFRITKGILDVSEDNALNR
                     KLVDHRIPHRNIIYIGDGMTDVPVMLLVKQNGGHSIAVYPKGEREKVEYLYTDGRVNF
                     VARADYTEKSDINSIVRLIIDQVSIAEQIEKKKQLLN"
BASE COUNT          532 a          326 c          326 g          623 t
ORIGIN      
        1 gcaaggtaat ttaattcatc taagttttct ttttcactag gaatgacgac atcttggttt
       61 ggattttcaa caaagagcgt tgtctcaaat aaaataggag atccgtcttg aataaattgt
      121 cctaagctat gtaaatcagt ggagaaggta actgaagttg ggaataaacc tttcttttct
      181 ttaccttcgc tttcgccata tagttgtttt aaccactcat taaacatagc gtattgtggt
      241 tcgtaaacaa catagagctc gctcttgtaa ttttgacgat agagtgtgtc gcgaataact
      301 gcatatttat aaaggtcatg atctaaatta tcgacgcttt cgctgcgtgc tttaagagcc
      361 cccgccatta agtcatcaat atcaattccc gcaacagcaa gtggtaaaag tccaacagcg
      421 gttaaaacac tataacgacc accgatatcg cttggcaatg taaaagtagt gtagccattt
      481 tgtgtcgcta gtttacggag cgcaccagtt ttagcgtctg tagtcgcaaa aattgcctta
      541 ttagcatttg cttcaccgac tttttcgatt aatagttgct ttaataagcg aaaagcaata
      601 cttgtttcag tagtggtacc acttttagaa ataacattaa tcgcaaagtt ttttgttttt
      661 aagtattcga gcgtttgcgc tgttaaggtt ggagaaaaag tatgacccaa aaagataatt
      721 tcaggtcctt tttgcggcaa taatccgtta attgcttcaa ttgcagctct tgcccccaag
      781 taagaaccac caataccagt aacgactaaa gtatcaaagg tttctctaat cattaaggct
      841 gtttctttaa ttaaattaaa ttctttttta tcgtaatgta atggccaatc agcccaacca
      901 agataatcat ttcctgctcc ctttttttga ttaatgcctt cattaatttt ttcaagttta
      961 gagcggtatt tttcaaaatc aatcgtagct aatacattgt cataatttaa tctaatcatt
     1021 ttgcgcctcc ttaatagcct tttcaatcca aagcggcaat gctttttcaa gtgcactaaa
     1081 atcagtttgt gccggattaa tgtttgttgc ttttttatcg cgttcaaaaa taatgcgttc
     1141 attgtaagga acggatttaa gtgaaagttt taagaaacca ttatgttcat caatttcaat
     1201 tatccgcact ttaatttgtg catcaagttg caagtatgtg ttgatgttac taatgtaggc
     1261 atcactaatt tgttttatat gtaaaagccc agtcctattt tcaggaaagg taataatggc
     1321 accatatggt ctaatttcag taatagtgcc ttcgacaatt tcatttagtt cgtaaatgtt
     1381 cataactagt taagaagttg tttcttcttt tcaatttgtt cggcgataga cacttggtca
     1441 ataattaagc gaacaatgga attaatatca cttttttctg tataatcagc gcgagcaaca
     1501 aagtttaccc ggccatcagt gtataagtac tcaacttttt cgcgttcacc ctttggataa
     1561 acagcaattg agtgaccgcc attttgttta acaaggagca ttaccggaac atcagtcatc
     1621 ccgtcgccga tataaataat gttgcgatgt gggatgcgat ggtctactag tttacggttt
     1681 aaggcattat cttcgctgac atctaagata cctttagtaa tgcggaagta gaactgtgtt
     1741 ttagcggtgt agttaatcgc tagttttggc catactggta atttagtttg tggatcaaag
     1801 taaaatt
//
LOCUS       DAOSEX010000190         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_66304_length_1804_cov_3.935392, whole genome shotgun sequence.
ACCESSION   DAOSEX010000190 DAOSEX010000000
VERSION     DAOSEX010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66304_length_1804_cov_3.935392"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PK612_03755"
     CDS             complement(<1..692)
                     /locus_tag="PK612_03755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH95255.1"
                     /translation="MKAMRRLAKERTYEYLRLTYFVRFLGDAFFYTFLYVFLASLGFG
                     TDKLGLIAALSPFAALLGNITFQRIAKNLEVNRILMFIFSIIEMTIALIFFVFPKQPF
                     VFYAIIITCVSTINGPFYSLLDGYSGTYISERNKQYSSMRVMGTLSYVIGPLIGGFLV
                     DNTGAGFQTLFLMSSVFYIITSFLIYYLPKQKIELHIIVPPEEKKRLKIIGKPELIAY
                     LLFNFFVIGVTVV"
     gene            755..>1804
                     /locus_tag="PK612_03760"
     CDS             755..>1804
                     /locus_tag="PK612_03760"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH95256.1"
                     /translation="MKVKLVAQLRFLLSLFELLFGELQEIPGSTLGDILVLLNTIVLS
                     FLSVLYFYQVLLSIFSVVVRAKKYPETDVFPTYTFVTCARNEETVIAQLITTIRALDY
                     PQEKINIHVVADNCTDNTAIIAAQLKAKVYTRNDLTKIGKSYALEYYFNEQKKVVPTK
                     DFAGYIVIDTDNVLDAQFLKEVNKVYQATNAKVVATYRASTNAGDSLWAFGTGYSFLR
                     ECCLMHKGREVLGLSSYVSGTGFFVSYEKMKEVEGWNHHLLIEDIEFSAAHVMEKGRT
                     HYAHNAIFYDEQAVNFKSSWHQRMRWVKGLFQVSKVYTWSLFKSIFARKRTLKERFSS
                     YESFLFSTPFPAYIIW"
BASE COUNT          614 a          299 c          334 g          557 t
ORIGIN      
        1 acaacagtaa ccccaatgac gaaaaagtta aagagtaaat aggcaattag ttcgggtttt
       61 ccaatgattt taagcctctt tttttcttcg ggtggaacaa taatatgtaa ttcaattttt
      121 tgcttgggta aatagtaaat gagaaaagaa gtaatgatgt aaaacactga tgacatgaga
      181 aataatgttt gaaatccagc gccagtgtta tcaactaaaa acccgccaat aagtggacca
      241 atgacatatg ataatgtacc catcactcgc atcgatgagt attgtttatt acgctctgaa
      301 atatatgtcc cgctataacc gtcaagtagt gaataaaaag gaccattaat cgtacttaca
      361 catgtgatga taattgcata aaatacaaaa ggttgtttag ggaaaacaaa gaaaataagc
      421 gcaatagtca tttcaatgat tgaaaagata aacatcaaaa tacggttaac ttctaaattt
      481 ttcgcaatcc tttggaaagt aatattacca aggagagcgg caaacgggga aagagcagca
      541 ataagtccta atttatcagt gccaaagcca agcgacgcta aaaagacata caaaaatgta
      601 taaaagaaag catcgccgag aaaacgtacg aagtaagtta gtcttaagta ttcatatgta
      661 cgctctttag cgagtcttct cattgctttc ataatatcaa ctaaatgtta tcatttttcg
      721 atagtattta ccccaaaata gatataataa ttttatgaaa gtaaaattag ttgcgcagtt
      781 gcgttttctt cttagccttt tcgaactatt atttggggaa ttacaagaaa ttccgggctc
      841 aacacttgga gatatattag ttttattaaa cacaattgtc ttatcctttc ttagtgtttt
      901 atatttctat caagtgctac taagtatctt ttctgttgtt gttcgcgcca aaaaatatcc
      961 agaaactgat gtatttccta catatacatt cgtgacatgt gcgcgaaatg aagaaacagt
     1021 gattgcgcaa cttatcacaa caattagagc gcttgattat ccgcaagaaa aaataaatat
     1081 ccatgttgtt gccgataatt gtactgataa taccgcaatt attgctgcgc agttaaaagc
     1141 gaaagtttat acacggaacg atttaacaaa aattggtaaa agctatgcac ttgaatatta
     1201 tttcaatgag caaaaaaaag tagttcccac aaaagacttt gccggctaca ttgtgattga
     1261 taccgataat gtgcttgatg cgcaatttct aaaagaagtg aataaagtct atcaagcaac
     1321 aaacgctaaa gttgtcgcga cttatcgcgc tagtacaaac gctggtgatt cactttgggc
     1381 atttggaacg gggtactctt ttttacgtga atgttgctta atgcataaag gtcgtgaggt
     1441 gttaggtctt agttcttatg tatcaggcac aggttttttt gttagttacg aaaaaatgaa
     1501 agaagttgaa ggctggaatc accatttatt aattgaagac attgagtttt cagcagcgca
     1561 cgtcatggaa aaaggccgga ctcattatgc acataacgcg attttctatg atgagcaagc
     1621 tgttaacttt aaatcaagtt ggcatcaacg gatgcgctgg gtaaaaggat tgtttcaagt
     1681 ttcaaaagtt tacacttggt cattatttaa gtccattttt gcccgcaaaa ggacactaaa
     1741 agagcgtttt tcttcttatg aatcgtttct tttttcgact ccgtttccag cttacatcat
     1801 ttgg
//
LOCUS       DAOSEX010000191         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_67515_length_1782_cov_3.024899, whole genome shotgun sequence.
ACCESSION   DAOSEX010000191 DAOSEX010000000
VERSION     DAOSEX010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67515_length_1782_cov_3.024899"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PK612_03765"
     CDS             <1..227
                     /locus_tag="PK612_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95257.1"
                     /translation="VTFVTRSVGIATYVFLGSTFNYQNFTVLHWYQFIVIIGLLLYLV
                     FFLADKLEKYLESTPLSNFSFWRWSTRRKR"
     gene            376..1014
                     /locus_tag="PK612_03770"
     CDS             376..1014
                     /locus_tag="PK612_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH95258.1"
                     /translation="MKKFSIVVNSSVDLTEELRKELDLVVAPLKFIMNGQEYINYLDY
                     RNLGVKEFYQKLRDGVVATTSQVNINEYVDIIEKEFKKGNDVLILSLSSALSSNYNSA
                     RLAVDMFKDHPEHKVYLVDTLTASLGEGLVAYAASMKRKEGATLEETYQFIEQFKSNV
                     STWFTVDDLMFLKRGGRLGGVSAIFGTLLAIKPILHIDLEGRLIPMEKNTRS"
     gene            971..1249
                     /locus_tag="PK612_03775"
     CDS             971..1249
                     /locus_tag="PK612_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH95259.1"
                     /translation="MKGVSFQWKKTRGRKKALRIIADKVKETYDPSLPNFCYVAHSDD
                     EEAANLLKDMIEGLGIGLKVTLVNYVGPVIGAHVGPGTIGAFFFAKHR"
     gene            1416..>1782
                     /locus_tag="PK612_03780"
     CDS             1416..>1782
                     /locus_tag="PK612_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95260.1"
                     /translation="MKTLNEGESNTIKKPASALEKVLVILLVVFLGATITMLVFYHFA
                     RWGLGLNIFVFLLILIVPALLLLGVIIIIVNIAGKRNFEISWKRPLLPTFIVLGAGVF
                     SLLFGIGERATFKIRYTFTI"
BASE COUNT          563 a          256 c          336 g          627 t
ORIGIN      
        1 ccgtgacttt tgtgacgcgt tcagtgggaa ttgcgaccta tgttttttta ggtagtactt
       61 ttaattatca aaactttacg gtgctccact ggtatcaatt tatcgtaatt attggcctcc
      121 ttttatatct tgtttttttc cttgcggata aacttgaaaa atatcttgag tcaaccccgc
      181 tcagtaattt ttctttttgg cggtggtcaa cacgaagaaa acgctaaata tatacacgta
      241 ttaaaataag cttttataat ttgcgtaatg aacaattatg aaaaacattc acttttgtgg
      301 actttcaaat tttttctcgt atataattag atggctatta aatattaata gcgctacaaa
      361 aggagaaatt tatttatgaa aaaatttagt attgttgtta actctagtgt tgacttaact
      421 gaagagttac ggaaagagtt agacttagta gttgctccgc ttaagtttat tatgaatggt
      481 caagagtata ttaactattt agactatcgt aatttaggag tcaaagaatt ttatcaaaag
      541 ttacgtgatg gtgttgtcgc tacgacatcg caagtaaaca ttaatgaata tgttgatatt
      601 attgaaaaag aatttaagaa aggtaatgat gttttaattc tttcactttc aagtgcactt
      661 tcaagcaact ataatagtgc acgtcttgct gttgatatgt ttaaggatca tcctgaacat
      721 aaagtttatc ttgttgatac tttaacagca tcacttggtg aaggcttagt cgcttacgct
      781 gcttcaatga aacgaaaaga aggagcgaca cttgaagaaa cttatcaatt tattgaacaa
      841 tttaaatcca atgtctcaac gtggtttacg gttgatgatt taatgttttt aaagcgtggt
      901 ggtcgtttag gtggagtgtc tgctatattt ggtacacttt tagcaattaa accaatctta
      961 cacattgacc ttgaagggcg tctcattcca atggaaaaaa acacgcggtc gtaaaaaagc
     1021 tttacgcatc attgccgata aagttaaaga aacttatgac ccaagtctcc ctaatttctg
     1081 ttatgttgcg catagtgatg acgaagaagc ggcgaattta ttaaaagaca tgattgaagg
     1141 cttaggaatt ggtttaaagg ttactttagt aaattatgtt ggaccagtta ttggggcgca
     1201 tgtcggccca ggcacgattg gtgcattctt ctttgcaaaa caccgttaaa tttgatttaa
     1261 aagtaacgaa aggcttgcaa attgtaagta aaattcataa acgaaagaaa caattaactt
     1321 gttcattgaa ttttatgtta agttaattaa aaagtctttg ttatgaaaat attattagca
     1381 aaagtgcaaa tttattaatt tgctataatt tgattatgaa aactttgaat gaaggagaaa
     1441 gtaacacgat aaaaaagccc gctagtgcac ttgaaaaagt cttagttatt ttacttgtgg
     1501 tatttttagg cgcgactata acaatgcttg tcttttatca ctttgcgcgc tgggggttag
     1561 ggcttaatat ttttgtattt ttattaatat taattgtacc agcgctttta ctacttggtg
     1621 tcatcattat tatcgtcaat atcgctggga aaagaaattt tgaaatatcg tggaaaagac
     1681 cactattgcc aacatttatt gtacttggtg cgggagtatt ttcactttta ttcgggattg
     1741 gagaacgggc tacttttaaa atccggtaca cttttacgat tg
//
LOCUS       DAOSEX010000192         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_67648_length_1780_cov_2.907246, whole genome shotgun sequence.
ACCESSION   DAOSEX010000192 DAOSEX010000000
VERSION     DAOSEX010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67648_length_1780_cov_2.907246"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="PK612_03785"
     CDS             <1..652
                     /locus_tag="PK612_03785"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOH95261.1"
                     /translation="VRPIINEFKKQYAHSTFKKVLISSVVPKLNYPMSFVIKEILGVD
                     AEIVLPNPKTSVRLDVDVPESVGSDIVACAVGAYKFGKGPFIVIDLGTANKYFYVDDK
                     HVFHGVAISPGVEVSFKGLVKNAAQLFNVRLTLPAKVLGKNTPDSLNSGLLYGLLGEM
                     NGFINLIKKEVGDNPIVLLTGGNARYIKGAVDESVNYVPYLVHYGLYDMLVNGNEK"
     gene            642..>1780
                     /locus_tag="PK612_03790"
     CDS             642..>1780
                     /locus_tag="PK612_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH95262.1"
                     /translation="MKNKKLVSDIAIDGLFIALILVLSLVPYLGFIQIGGISATILPI
                     PVILGAALLGPRRGVLYGAAFGFSSFLFAVIRGTAGDALFVDPLISIVPRILFGFCTA
                     IFSAVSFNERTSFKLKRFLIFPYSAIMMLLHSFFVLLAMYLRYVNAFMEYIFPILTPL
                     VLLEALVATVIVPVLYNVLYIPFEKYKDKFTTKNKSIYGTITSVYFADALNSLKEFVS
                     INSVYDEKTVTKKTPYGKGVNEALEYMKNLATNDGFEAKIIDGRVVEIFVGEKYNKNI
                     AVFAHADVVPATGEWDTPPFTADIREGKLYGRGTSDDKGPAIAAYYAIKALNDNNLLI
                     NYSVRLVIGGDEERGSSCMHYYFNEYNAPAPVHGFTPDAEFPLIYG"
BASE COUNT          517 a          262 c          372 g          629 t
ORIGIN      
        1 cgttcgtccc atcattaacg aatttaaaaa gcaatatgcg cacagtacat ttaagaaagt
       61 gcttatttct agtgttgtcc cgaagttaaa ttatccaatg tcatttgtga ttaaagaaat
      121 tttaggagtc gatgctgaga ttgttttacc aaatcctaaa actagtgtga gactagatgt
      181 tgatgttccc gaaagcgttg gtagtgatat tgtggcttgc gctgttggtg cttataaatt
      241 tgggaaaggt ccttttattg ttattgattt agggacggct aataaatatt tttatgttga
      301 tgataaacat gtttttcacg gagtggcaat tagtcctggt gtggaagtat cgtttaaagg
      361 tttagtaaaa aatgcagcac aattatttaa tgtccgcctt actttacccg cgaaagtact
      421 cggtaaaaat acacccgact cgcttaatag cggtttatta tatggtttat taggggagat
      481 gaacgggttt attaatttaa taaaaaaaga agtaggagat aatccaattg tgttattaac
      541 tggtggtaat gcacgctata ttaaaggtgc tgtggatgaa agtgtaaatt atgttccata
      601 tttagtgcat tacgggttat atgacatgtt ggtaaatggc aatgaaaaat aaaaagttag
      661 taagtgatat tgcaattgat gggttattta ttgcccttat tttagtgtta agtcttgtcc
      721 cgtatttagg ctttattcaa atcggaggga tttcggcgac aatcttacca attcctgtaa
      781 ttttaggagc ggcactttta ggacctagac gcggtgtgct ttatggcgct gcttttggtt
      841 tttcgtcttt tttatttgct gttattcgtg gtactgctgg ggacgcatta tttgttgatc
      901 ctttaattag tattgtgccg cggatattat ttggattttg taccgcaata ttttcagcgg
      961 tttcatttaa tgaacgcact agttttaaat taaaacgttt cctgatattc ccttattcag
     1021 caattatgat gctgttacat tcattttttg tcttacttgc aatgtatttg cgttatgtaa
     1081 atgcttttat ggaatatatt ttcccgattc ttactccgct tgtattactt gaagctttag
     1141 tggcaactgt tattgttcct gttttataca atgttttata cattcctttt gaaaaatata
     1201 aggataaatt cactacgaaa aataaatcaa tttatggaac gattaccagt gtttattttg
     1261 ccgatgcttt aaattcttta aaagaatttg tgagtattaa ttcggtatat gacgaaaaaa
     1321 cagttacgaa aaagacacca tatggtaaag gggttaacga agcattagag tatatgaaaa
     1381 atcttgccac aaatgacgga tttgaagcga aaattattga tggccgtgtc gttgaaatat
     1441 tcgttggtga aaagtataac aaaaatattg ctgtttttgc tcacgctgat gtcgtgccag
     1501 caactggcga atgggataca ccaccattta ccgctgatat tcgtgaagga aaactctacg
     1561 gccgtggcac aagtgatgac aaaggaccag cgattgctgc ttattacgca attaaagctt
     1621 tgaatgataa taacttatta attaattata gtgtccgtct tgtaattggt ggcgacgaag
     1681 aacggggtag cagttgtatg cactattact ttaatgaata taatgcgcct gcaccggtac
     1741 acggttttac acctgatgcg gagtttccgc taatatatgg
//
LOCUS       DAOSEX010000193         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_67654_length_1780_cov_2.720000, whole genome shotgun sequence.
ACCESSION   DAOSEX010000193 DAOSEX010000000
VERSION     DAOSEX010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67654_length_1780_cov_2.720000"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PK612_03795"
     CDS             <1..1111
                     /locus_tag="PK612_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95263.1"
                     /translation="FGLPFNKSTEVMIANQSFFDAAKEKDPSIFIPKTWQDLAVVGPK
                     LKAVAKDNGWFGKLVKKDGTAVAKPAEFDAALKEQVAVDMTLVNENQFIPFSWDSASN
                     FFITIIRQWGAMYTEKGNTIQNGYIRFQEEPHRTKTLAALSYFKELYNAGLIGLPDNF
                     GEPLYSSTPFKEGKLVLTISSSAGVGENLPGGTTEYPFELSINPIPYNGDLAAQKSVI
                     AQGTNLALFTVGQGRDPKAQAERLAAWRLLRFLTYEANYEFGKATSYFPVLDASKIDR
                     DSQRYQDYLMYSEFLDQTDGTPREKVIRSTAKLQANVYMDPEAGWNKFVDPGFLGSSE
                     IRNTIESVMAELFKDKTPEQVLNDTVTKLARYVQK"
     gene            1126..>1780
                     /locus_tag="PK612_03800"
     CDS             1126..>1780
                     /locus_tag="PK612_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95264.1"
                     /translation="MKKTFVKIGLLLAVFGLLVGCDNSSSEVVPPSSASEEVVSSSEE
                     EVKLTEQEYQIAVNYIVRSVPLVLERGVKVPTNVERAEAENPLPTPELKYGDNLLLIT
                     AGELKYEDEINGVYLYPEYTVTYSFLEQEPYAGFTFEYDDIGRYFAVPEYPRYEAEYD
                     NGGNPIHTVPPAKAARLYVTIHIEERFTTFNMDMILEPQAIIHDYNLADVRSVPTGSV
                     "
BASE COUNT          569 a          348 c          367 g          496 t
ORIGIN      
        1 attcggctta ccgtttaata aatcaacgga agtaatgatt gctaaccaaa gtttctttga
       61 tgcggctaaa gaaaaagacc caagtatctt tattccaaaa acatggcaag atttagccgt
      121 tgttggccca aaacttaaag ccgtcgcgaa agataacggc tggtttggta aattagtcaa
      181 aaaagatgga acagcggtag caaaacctgc ggaatttgat gcagcgctta aagaacaggt
      241 cgctgttgat atgacgctag ttaatgaaaa tcaatttatc ccctttagct gggatagcgc
      301 tagtaacttc tttattacaa ttattcggca atggggtgca atgtatacgg aaaaaggtaa
      361 cacaattcaa aatggttaca tccgtttcca agaagaaccg caccgcacta aaacactagc
      421 ggccctatct tattttaaag agctatataa cgctggatta attggtctac ctgacaactt
      481 tggtgaaccg ctttattctt caacaccatt taaagaagga aaactcgtac ttactattag
      541 tagtagtgcc ggtgttggtg aaaatttacc aggcgggaca actgaatatc catttgaatt
      601 atcgattaat ccaattcctt ataacggtga cttagcagcg caaaaatcag ttattgccca
      661 aggcacaaac ttagcgctct ttaccgttgg tcaaggccgt gatccaaaag cacaagctga
      721 acggctcgct gcctggcgct tacttcgctt cttaacttat gaagcaaact acgaatttgg
      781 taaagcaact tcctacttcc cagtgttaga cgcttccaaa attgatcgcg atagtcagcg
      841 ttatcaagat tacttaatgt atagtgaatt cttagatcaa acagacggga caccacgcga
      901 aaaagtaatt cgtagtaccg ctaaacttca ggctaatgtg tatatggatc ctgaagcagg
      961 ctggaataag ttcgttgacc cagggttcct tggttcaagt gaaattcgta ataccatcga
     1021 atcagtgatg gctgaattat ttaaagataa aacaccggag caagtgttaa atgacactgt
     1081 tactaaactt gcgcggtatg tacaaaaata ataaaggaga aatatatgaa aaaaacattt
     1141 gttaaaattg gcttattact tgcggttttt ggcttacttg ttggttgtga taatagcagc
     1201 agcgaagtag tgccgccctc aagcgccagt gaagaagtcg tttcatcaag cgaagaagaa
     1261 gtaaaactaa ctgagcaaga atatcaaatt gccgttaatt acattgtgcg cagtgttccg
     1321 cttgtccttg aaagaggcgt taaagttcca acaaacgttg aacgggctga agcggagaac
     1381 ccactaccga caccagaatt aaagtatggt gacaacttat tacttattac cgctggtgaa
     1441 ttaaagtatg aagacgaaat taatggcgtc tatctttatc cagaatatac tgtaacctat
     1501 tcattcttag agcaagaacc ttatgcaggc tttacttttg aatatgatga tattggccgt
     1561 tattttgctg ttcctgaata tccgcgctat gaagcggaat atgacaatgg cggtaaccca
     1621 attcacactg taccaccggc aaaagcagcg cggttatatg tcacgattca tattgaagaa
     1681 cgtttcacta catttaatat ggacatgatt ttagagccac aagccattat tcatgattac
     1741 aaccttgctg atgtccgtag tgtgccaact ggctcagttg
//
LOCUS       DAOSEX010000194         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_67831_length_1777_cov_2.282811, whole genome shotgun sequence.
ACCESSION   DAOSEX010000194 DAOSEX010000000
VERSION     DAOSEX010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67831_length_1777_cov_2.282811"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PK612_03805"
     CDS             complement(<1..442)
                     /locus_tag="PK612_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH95265.1"
                     /translation="MFIIALDIGGTNIRAAIADQNGKLLEVVKEKTLRHDKEIFITQV
                     KGIITQLDLSKYDPIAISIGVPGRVRQNGFIDELPNIGITNIDLVSALQKTFKLPVSV
                     RNDAEMAAFAEAALGAGKELKSTYFVTISTGIGGCLIVDRTIKNT"
     gene            complement(442..>1777)
                     /gene="pyk"
                     /locus_tag="PK612_03810"
     CDS             complement(442..>1777)
                     /gene="pyk"
                     /locus_tag="PK612_03810"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOH95266.1"
                     /translation="NFSHGTYETHKTIIDISKKLIEEHIYIPIMLDTAGPEIRCHFFE
                     DGKVEIVKDSIVTINMKEVLGTATEFSVNYPKLVDDILVGDMIKIDDGKLRLDVIEVD
                     REKRLIKVKAFNTHVISNRKGINIPGERMTLPALSERDKKDIKFGIENGIDMIAASFT
                     RCAADVIQLKNYLAELGAPEIPIIAKIENKQGVDNIKEILEVADGIMVARGDMGVDIP
                     PEVVPVMQSDIILECRKVGKPVIVATQMLDSMQTNPLPTRAEVSDVATAIKEGADAVM
                     LSAETASGLYPVESAAMQQRIAITMEEFLPYTQLANFGYNYSKKKRSDAIATSVASTA
                     NLIDAKAIVCFSETGKSAICISKSRPKCPIIMVTSSPAAGMRVAPYFGIYSIVVKTLP
                     QLIEDMEAFALIKMRQLGLKPNDNIIITGGTPTGTGSTNFLRIVALNKLGDI"
BASE COUNT          511 a          372 c          319 g          575 t
ORIGIN      
        1 tggtgttttt aattgtgcgg tcaacaatta ggcagccgcc aatgccagtg ctaatagtga
       61 caaaataagt gctttttaat tctttgcccg ctcctaatgc tgcttcagca aaagcagcca
      121 tttctgcatc attacggact gaaacaggga gcttaaaagt cttttgcaaa gcactaacta
      181 aatcgatatt agtaatgcca atatttggta attcatcaat aaatccgttt tgtctaacgc
      241 gacccggtac accaatggaa atagcaatag gatcatattt acttaaatca agttgtgtaa
      301 taatcccctt aacttgcgtg ataaaaattt ctttatcatg gcgcagtgtt ttttctttta
      361 caacctcaag taatttcccg ttttggtcag cgattgctgc gcgaatattt gtgccaccaa
      421 tatcaagtgc aataataaac attaaatatc tcctaattta ttaagggcga caatccgtaa
      481 gaagtttgtc gaaccagtgc cagttggcgt accaccagta atgatgatat tatcatttgg
      541 ttttaaccca agttgtcgca ttttaatgag cgcaaaagct tccatatctt caattaattg
      601 tggtagtgtt tttaccacga tactataaat gccaaagtaa ggtgcaactc gcattcccgc
      661 cgcgggacta ctcgtgacca taatgatcgg acacttagga cgtgatttag atatgcaaat
      721 cgcactttta cccgtttcac taaaacaaac aatcgcttta gcgtcgatta aattcgccgt
      781 tgaggcgacg cttgtggcaa tcgcgtcact gcgctttttc tttgaatagt tatagccaaa
      841 gtttgcaagt tgagtatatg gtaaaaattc ttccatagta atggcaatcc gttgttgcat
      901 tgctgcactt tcaaccggat ataaaccgct agccgtttcc gcactaagca tcacagcgtc
      961 agcgccttct ttaatggcag tcgcgacgtc acttacttcc gcccttgttg ggagcggatt
     1021 agtttgcatt gagtcaagca tttgtgtagc aacgataact ggtttgccga ccttccgaca
     1081 ttctaaaatg atatcgcttt gcatcacagg aacgacttca ggtggaatgt caactcccat
     1141 atcaccacgg gcaaccatga tcccatcagc gacttctaag atttctttaa tattatcaac
     1201 accttgtttg ttttcaattt tagcgataat cggaatttcc ggtgcaccta gttccgctaa
     1261 ataattttta agttgaataa catcagcagc acacctagta aaacttgccg caatcatgtc
     1321 aatgccgttt tcaattccaa atttaatatc ttttttatcg cgttcactta gtgctggtaa
     1381 agtcattcgt tcaccaggaa tattaatgcc tttacgattg gaaataacat gcgtattaaa
     1441 tgctttaact ttgattaaac gcttttctct atcgacttca ataacatcaa gtcttaactt
     1501 gccatcatca atcttaatca tgtcaccgac gagaatatca tcgactaatt taggataatt
     1561 aacactaaat tctgtagcgg tgcctaatac ttctttcata ttgatggtga cgatggagtc
     1621 tttgacaatt tcaactttcc catcttcaaa gaagtgacaa cgaatttctg gtccagctgt
     1681 atcaagcata atcggaatat aaatatgttc ttcgattagt tttttggaaa tatcaataat
     1741 tgttttgtgt gtttcataag tgccgtgcga gaagttg
//
LOCUS       DAOSEX010000195         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_68741_length_1762_cov_2.855302, whole genome shotgun sequence.
ACCESSION   DAOSEX010000195 DAOSEX010000000
VERSION     DAOSEX010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68741_length_1762_cov_2.855302"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PK612_03815"
     CDS             complement(<1..476)
                     /locus_tag="PK612_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOH95267.1"
                     /translation="MGLSVGIIGLPNVGKSTLFNALTASKVEAANYPFATINPNKGTV
                     RVLDPRVTKLAAYYEPEKTIYATIEFNDIAGLVRGANRGEGLGNQFLGHIRECDVILE
                     VVRCFSGEEIIHVEQSVDAKRDIEIINLELILKDLEVLYRRKDKVETKARVNKDKDS"
     gene            complement(580..777)
                     /locus_tag="PK612_03820"
     CDS             complement(580..777)
                     /locus_tag="PK612_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOH95268.1"
                     /translation="MKGTIKQFNQERGYGFIRAEDGTDVFFHYSSLVMDGFKTITAGE
                     KVEFTLEQSDRGPRASNIVKI"
     gene            complement(892..>1762)
                     /locus_tag="PK612_03825"
     CDS             complement(892..>1762)
                     /locus_tag="PK612_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95269.1"
                     /translation="LELPEGILNFALQYNGFSSFNYRNANTALQIDELIISSGLAHKL
                     GLSKDAQNTELYIGALVAIAEGSDGYLKEYETIKISIKKVIDNDEVVIYHHPLWAYLL
                     YKDVFNVFPFALEITGAIYQSVNNEDMKNLQNRFSVSYPYKDFSVLVADTLNELERYT
                     FLASVGAFLLAALITFMTIYLLVAETSDQFSGLFLVGYNEEGVHGVISRYILRFLGVI
                     ILLAIVQLFILSFVVEVVLMKYLDTSFSYVFNPKPYLLVLAFATGLLIFLLTYFKFQY
                     EKLDFLAFSKRDL"
BASE COUNT          617 a          336 c          257 g          552 t
ORIGIN      
        1 gaatctttat ctttattaac gcgcgccttt gtttcgactt tatctttacg acgatagagc
       61 acttctaaat ctttgagaat tagttccaaa ttaataattt caatatccct tttagcgtca
      121 acactttgtt caacatgaat tatttcttcg ccgctgaagc aacgcactac ttctaaaata
      181 acatcgcatt cgcgaatatg gcctaggaat tggttaccaa gaccttcacc gcgattagcg
      241 ccccgcacta aaccagcaat atcattaaat tcaatcgttg catatattgt tttttcaggt
      301 tcataatacg ctgcgagttt tgtgacacgg ggatcaagta cacggacggt acctttattt
      361 ggattaatcg tcgcaaaagg ataattagca gcttcaactt tactagcggt taaagcatta
      421 aataatgttg atttgccaac attaggtaat ccgataattc caacacttaa tcccataatt
      481 ttctcccgca gtattatatt actattatgc tttttaagca ataatgacaa taaaaggctg
      541 ctcataagaa cagcctctta tttactcatt taagttaaat tatatcttaa caatattaga
      601 tgcgcgtggt ccgcgatctg attgttctaa ggtaaattcg actttttcac cagcagtaat
      661 ggttttgaag ccatccatta caagtgatga gtagtggaaa aagacatcag tgccgtcttc
      721 tgcgcgaata aagccgtaac ctctttcctg attgaattgt ttaatcgtcc ccttcatcag
      781 taatccttcc atgacatatc gtctatttgt gtctaagcac aatagaatta taacaaaaac
      841 aaaaatattc ggtgcttaaa ataacaaata aaaagtaaga aagtcgtaaa gttataaatc
      901 acgttttgaa aacgctaaaa aatcaagttt ttcatattga aatttaaaat aagttagtaa
      961 gaaaattagt aatcccgtcg caaatgctaa aacgagtaaa tatggttttg gattaaaaac
     1021 atagctaaaa ctagtgtcta aatatttcat cagcactact tcaacgacaa acgacaaaat
     1081 aaataattgc acaatcgcta aaagaataat gactcctaga aagcgcagga tatatctact
     1141 aatcacccca tgaacacctt cttcgttata accaactaaa aagagcccgc taaattggtc
     1201 gcttgtttca gcaactaata agtaaattgt cataaaagta ataagcgccg ctaaaagaaa
     1261 agcaccaact gatgctaaaa atgtataacg ctctaactca tttaatgtat cggcaacaag
     1321 cactgaaaaa tctttataag gataagagac actgaagcgg ttttgtaaat ttttcatgtc
     1381 ttcattattg acactctgat aaatagcgcc cgtaatctct aaagcaaatg ggaaaacatt
     1441 gaatacatct ttataaagta aatatgccca taacggatga tggtatatta caacttcatc
     1501 attatcaatc acctttttaa ttgatatttt tattgtttca tattccttta aataaccatc
     1561 acttccttcc gcaatagcta caagtgctcc aatatataac tctgtatttt gtgcatcttt
     1621 actaagccca agtttatgtg ctaaccctga agaaataatt aattcatcaa tttgtaaagc
     1681 tgtattagcg ttacgataat taaaactaga aaagccatta tattgaagcg caaaatttaa
     1741 aataccctca ggcaattcta ag
//
LOCUS       DAOSEX010000196         1762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_68743_length_1762_cov_2.841828, whole genome shotgun sequence.
ACCESSION   DAOSEX010000196 DAOSEX010000000
VERSION     DAOSEX010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68743_length_1762_cov_2.841828"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..666
                     /locus_tag="PK612_03830"
     CDS             70..666
                     /locus_tag="PK612_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOH95270.1"
                     /translation="MLEQTGLIHEVGLWVIDQSLKQHKALVKKGFAPLRFSVNLSVAQ
                     FRRDDFVDNVLKLLKKHKVEPQYLELEITESLLSKNFADTIEKLTRLNDAGLHIAIDD
                     FGKGYSSLHRLQLVPFNRIKIDKSVVDNITIEPKKVVTAKTIINLAKSLNANITTEGV
                     ETKEQAAFMKDLGTDEIQGYYYSRPLPPKDLEDFLKQK"
     gene            complement(715..1320)
                     /locus_tag="PK612_03835"
     CDS             complement(715..1320)
                     /locus_tag="PK612_03835"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOH95271.1"
                     /translation="MHKVSIRSKLTPDQVFTIPNILSFFRLLLIPLIVFLYVGKELYG
                     WTLVVLIISLVTDIIDGLVARKLNMVTDFGKFIDPVADKLTQLAVLFCLVTRFPLMWI
                     PISIMLLKEILQFALRFVVFRYTGEVHSAEWHGKVATFLIYVMMSLHVIWYGITDVVS
                     TLTITITSLSMLLSCVLYTISSAVIIINIKRRKKDKEQLNN"
     gene            complement(1370..1454)
                     /locus_tag="PK612_03840"
     tRNA            complement(1370..1454)
                     /locus_tag="PK612_03840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1418..1420),aa:Leu,seq:cag)
     gene            complement(1484..>1762)
                     /locus_tag="PK612_03845"
     CDS             complement(1484..>1762)
                     /locus_tag="PK612_03845"
                     /inference="COORDINATES: protein motif:HMM:NF037476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95272.1"
                     /translation="DFMILLVEMKKHPVFAEISSFNIIGDGADDVIVSTLESRVGAAH
                     GFFNLGLKEKEVKTELKDGSYTNVLTNTDIVVNDGKIKVSEPIIIIEE"
BASE COUNT          611 a          335 c          280 g          536 t
ORIGIN      
        1 ccgcaaaaca aggaatagtt agtaagttga acacgctaaa ggccacaaca gcagtgttgc
       61 ttagtccaat tgcttgaaca aactggctta atccacgaag tcgggttatg ggtcattgac
      121 caatcactta aacagcataa ggcactagtg aaaaagggat ttgccccact gcgcttttcc
      181 gttaatctct ctgtagcgca attccgccgt gatgactttg tggataatgt attgaaatta
      241 cttaaaaaac ataaggtgga acctcagtat cttgaactag aaattactga aagtttgctc
      301 tcgaaaaatt ttgccgacac tattgaaaaa ttaacaagac ttaatgatgc gggattacac
      361 attgccattg acgattttgg taaaggatat tcttcactac accgcttaca acttgtccca
      421 tttaaccgaa ttaaaattga taaaagcgtc gttgataata ttacaatcga accgaagaaa
      481 gttgttactg ctaaaacaat tattaattta gcgaaatcat taaacgctaa tattacaact
      541 gaaggtgttg aaaccaaaga acaggcagcg tttatgaaag atttaggcac tgatgaaatt
      601 caaggctact actactcacg cccacttccg cctaaagatc ttgaagactt tttaaaacaa
      661 aaataatcac ttacttttaa ttttatatat aatgcactaa ttaattattg cttattaatt
      721 attaagttgc tctttatctt tttttcttct tttaatatta ataatgatta cggcactaga
      781 aattgtataa agcacgcatg ataaaagcat tgatagtgaa gtaattgtta ttgttaaagt
      841 tgacactaca tctgttattc cataccaaat aacatgtaat gacatcatta catagataag
      901 aaatgtcgct actttgccat gccactcagc gctatgaact tcaccagtat atctaaacac
      961 tacaaagcga agagcgaatt gaagtatttc ctttaataac attatgctaa ttggaatcca
     1021 catcagcggg aatctagtca ctaaacaaaa tagaaccgct aattgggtta atttatcggc
     1081 aacagggtcg atgaatttcc caaaatcagt caccatattt aatttacggg caactagacc
     1141 atcaataata tcagtgacta gtgaaatgat aagtacaaca agcgtccagc cataaagttc
     1201 tttaccaaca tagagaaaga cgataagcgg aataagtagt aagcggaaaa aggataagat
     1261 attaggaatg gtaaaaactt gatcaggtgt taatttactc ctaatactaa ctttgtgcat
     1321 agttttctcc tatttccttt atatttacac taattaaaat aattaaaatt ggtgcagcca
     1381 aagggacttg aacccccaca tcttgcgata caagcgtctg aaacttgcgc gtctaccaat
     1441 tccgccatga ctgcctgcac tttattttaa cactgcaaaa aatttattct tcaataatga
     1501 taataggttc actaactttt attttaccgt cgttaacaac gatatcagtg ttcgtcagca
     1561 cattagtgta acttccgtct tttaactctg ttttaacttc tttttcttta agccctaaat
     1621 taaagaaacc gtgtgccgct ccaactcttg actcaagcgt tgaaacaatt acatcatctg
     1681 caccgtcgcc aataatatta aaggaagaaa tctcggcaaa aactgggtgt ttcttcattt
     1741 caacaagtaa aatcataaag tc
//
LOCUS       DAOSEX010000197         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_68928_length_1759_cov_2.343310, whole genome shotgun sequence.
ACCESSION   DAOSEX010000197 DAOSEX010000000
VERSION     DAOSEX010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68928_length_1759_cov_2.343310"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="PK612_03850"
     CDS             complement(<1..162)
                     /locus_tag="PK612_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH95273.1"
                     /translation="MYDIIIIGAGVIGALIGREASKYELNILMLEKDNDVGNGTSAAN
                     SAIVHSGYDP"
     gene            complement(321..>1759)
                     /locus_tag="PK612_03855"
     CDS             complement(321..>1759)
                     /locus_tag="PK612_03855"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOH95274.1"
                     /translation="LKEKYTGKLTEAISVFVKLERSAAIEAVKGEMVEEYEAKEYESD
                     DDKRRVISDVKALHEVMVSDEIRRLITHEKVRPDGRKVDEIRPLDSQVDLLPRTHGSA
                     LFTRGQTQVLSVVTLGPKSDEQIIDNLTDEESRRFMHHYNFPPYSVGEIKRLGGPARR
                     EIGHGALGERALLAVIPSDEEFPYTIRVVSEVLESNGSSSQASICASSMALMAAGVPI
                     KDMVTGIAMGLITSGPLDGNHPYTILTDIQGLEDHFGDMDFKVAGTSKGITALQMDIK
                     VRGISKEVLAESLAQAKKAREEIRANQLSAISAPREDVAEFAPKMGKMTIDVDKIREV
                     IGTGGKVINKIIEDNDGAKIDIDDDGLVVVYHMNRATINNVLATIEAIVKEAKAGEIY
                     EGKVTRVEPYGVFVNLFGNVDALVHVSDLDWAYVDDASKLYKIGDTLKVKVTGVDEKG
                     KVKASVKVFKAKPEGYTERKSGGRRNNK"
BASE COUNT          506 a          401 c          273 g          579 t
ORIGIN      
        1 tgggtcataa ccactatgca caattgcgct attagcagca ctagtgccat tacctacatc
       61 gttatctttt tcgagcatta aaatattaag ttcatattta cttgcttcgc gtccaattaa
      121 agcgccgatt accccagcgc caataataat aatgtcatac ataaaaatta accttagagc
      181 aactccgtgc ttttctatat atatttatca tacactaatt ataaaaataa ttaggaatta
      241 aagccttaaa ttatttacat ctattaaaaa acctccaaac catatggtta agaggtttaa
      301 agaattttca atagtaatta ctatttatta ttacggcgac cgccactttt acgttcggtg
      361 tatccttctg gtttagcttt aaagactttt acactcgctt taactttacc tttttcatca
      421 acaccagtaa ctttaacttt taatgtatca ccaattttat ataactttga tgcatcatca
      481 acataagccc agtctaaatc actaacatgc actagtgcgt caacattacc aaagagatta
      541 acaaagacac cataaggttc aacgcgggtt actttacctt cgtaaatttc cccagccttt
      601 gcttctttaa cgattgcttc aatcgtggca agcacattat taatcgtcgc acggttcatg
      661 tgatagacaa cgactaatcc gtcgtcatca atatcaatct tcgcgccatc attatcttcg
      721 ataatcttat taatgacttt accgccagta ccgataactt cacggatttt atcaacatca
      781 attgtcatct tacccatctt tggtgcaaat tcagcgacat cttcacgtgg tgcactaatc
      841 gcacttaatt gatttgcgcg aatttcttca cgagcttttt tcgcttgcgc taagctttcg
      901 gctaagactt ctttgctgat tccgcgtact ttaatatcca tttgtaatgc agtaattcct
      961 ttactagtac cagcaacttt aaagtccata tcaccgaagt gatcttctaa gccttggata
     1021 tcagttagaa tggtataggg atgattacca tcaagtggtc cacttgtaat taagcccatg
     1081 gcaatcccgg taaccatatc tttaattggg acaccagcgg ccatcagtgc catacttgag
     1141 gcacaaatac tagcttgtga acttgaacca ttactttcaa gtacttcact tacaacacgg
     1201 atggtgtatg ggaattcttc gtcacttgga ataacggcaa gtaaagctct ttcacctaaa
     1261 gcaccatgtc caatttcacg ccgtgctgga ccacctaatc gtttaatttc gccaacgcta
     1321 taaggcggga agttataatg gtgcataaag cgccgtgatt cttcatcagt taagttatca
     1381 ataatttgtt cgtcactctt tggcccaagc gtcacaactg ataagacttg tgtttgtccg
     1441 cgggtaaaaa gtgcactacc atgcgttctt ggtaataagt caacttgact atcaagcgga
     1501 cgaatttcat cgactttacg accatcaggt ctaacttttt cgtgagtaat aagccggcga
     1561 atttcatcgc tcaccattac ttcgtgtaac gctttaacat cacttataac acggcgtttg
     1621 tcgtcatcac tttcatattc cttagcttca tattcttcaa ccatttcgcc tttaacagct
     1681 tcaattgctg cactgcgttc taatttgaca aagacactga tagcttcagt gagtttacct
     1741 gtatattttt ctttaagtt
//
LOCUS       DAOSEX010000198         1754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_69205_length_1754_cov_1.801648, whole genome shotgun sequence.
ACCESSION   DAOSEX010000198 DAOSEX010000000
VERSION     DAOSEX010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69205_length_1754_cov_1.801648"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PK612_03860"
     CDS             <1..636
                     /locus_tag="PK612_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOH95275.1"
                     /translation="GFVSETDALNLMAESHETMMRQLVKVAEDDDNLPPHKTALTRLP
                     WLLVAVVLNLLIAGTLTSYEGLLSTFVGLVLFQPMILGMAGNIGTQSMGVTILSLHSG
                     EIKIRKHFGRELLIGLLNSFASGIVGFGIVFLFLTILGQDNALMYAAVVGLSLMCAMI
                     ISSLAGVLTPIILRAVKLDEKAASGPLITTINDFFALQIYFLIATLLFTVL"
     gene            complement(704..1522)
                     /locus_tag="PK612_03865"
     CDS             complement(704..1522)
                     /locus_tag="PK612_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95276.1"
                     /translation="MKKILGAIGGFFVAIWRWIKETAWVQPLLIVGIIFGIIFAIPSV
                     VDGIRKIDERNNSAEKYYQQFQVSLAGAENSAADKLLDEIKQNSEGGSESLKGQKFFV
                     VFVQKDEACSACLDAREGFEYLADDGKALLDDGRKIELKTIFVDQELKRKDKEDWKKE
                     DSDPVDNYAETAFEAFLLRNAARFEEYAGDALNTHYYINDGITEQQVEDIESADVKRF
                     QTPTVLQIDFTDTAPQPGVTNVFIGVQGAKKLDRAKYIADAWNYKGQFGPNYTV"
BASE COUNT          539 a          324 c          301 g          590 t
ORIGIN      
        1 gggttcgttt ctgaaactga cgcattaaat ttaatggcgg aatcgcatga aacaatgatg
       61 cgccaattag tgaaagttgc cgaagatgat gataatcttc caccacataa aacagcctta
      121 acacggttac cgtggctgct cgtcgcggtc gtacttaact tactaatcgc tggcacactt
      181 acatcatatg aaggactttt aagtactttt gtgggcttag tcttattcca accaatgatt
      241 cttgggatgg ctggtaatat tggtacacaa agtatggggg tcacaatttt aagcttacat
      301 agtggtgaaa ttaaaattag aaaacatttt gggcgtgaac ttttaattgg attattaaat
      361 agttttgctt ccggtatcgt cgggtttggt attgttttct tattccttac tattttaggt
      421 caagataatg ctttgatgta tgctgcagta gtaggcttat cactcatgtg cgcaatgatt
      481 attagctcat tagcaggtgt gttaacgcca attattttgc gcgctgttaa gctagacgaa
      541 aaagcagcgt ctggtccgct tattacaacg attaatgact tctttgcctt acaaatttat
      601 ttcttaatcg cgacactttt gtttacagta ctttaataat ttagtagaag aagtaaaaag
      661 aaaagccggc ttaacgccgg catttttcaa cttttcaata atgttagaca gtataatttg
      721 gtccaaattg acctttatag ttccatgcat cagcaatgta tttagcgcga tcaagttttt
      781 tcgctccttg aacaccgata aagacatttg taacaccagg ttgtggtgct gtgtcagtaa
      841 agtcaatttg taacacggtt ggggtttgga aacgtttgac atccgctgat tcaatatctt
      901 caacttgttg ctcagtaata ccgtcattaa tataatagtg ggtattaagt gcatccccag
      961 catactcttc aaaacgagca gcgttacgaa gtaagaaagc ttcaaaagcg gtttcagcat
     1021 aattatcaac tggatcgcta tcttctttct tccaatcttc tttatcttta cgttttaatt
     1081 cttggtccac aaagattgtt ttaagttcaa tcttccggcc atcatcaagt aaagctttac
     1141 catcatccgc taaatattca aagccttcac gagcatcaag acatgcactg catgcttcat
     1201 ctttttgcac aaaaactacg aagaattttt gtccttttaa gctttcacta ccaccttctg
     1261 aattttgctt aatttcatca agtaatttat cagcggcact attttccgca ccagcaagtg
     1321 agacttggaa ttgttgatag tatttttccg cactattgtt acgttcatca attttacgaa
     1381 tgccatcgac gacacttgga attgcaaaaa taattccaaa gataattcca acaattaaaa
     1441 gtggttggac ccaggctgtt tctttaatcc agcgccagat agctacaaag aaaccaccga
     1501 tagcacctaa gatttttttc atgaaaagtt cctccttcta ttaaataata gagcacgtat
     1561 aataataaca ctctcaacat atattttcaa acaaaagcaa taataaaaaa agcaatttac
     1621 taaagtttat gaaatctaat agcaaaacaa tgtgtttttt cattaaaaca atagttttaa
     1681 atagttatat tttgcaagtt tattaaatta aacctataat tcaagatagt gaggtaatta
     1741 tgggtattcg tttt
//
LOCUS       DAOSEX010000199         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_69233_length_1753_cov_3.038869, whole genome shotgun sequence.
ACCESSION   DAOSEX010000199 DAOSEX010000000
VERSION     DAOSEX010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69233_length_1753_cov_3.038869"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /gene="glgP"
                     /locus_tag="PK612_03870"
     CDS             <1..1383
                     /gene="glgP"
                     /locus_tag="PK612_03870"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HOH95277.1"
                     /translation="AYTNHTIMVEALERWPVRYVHHLVPRNYMIIEEIHRRSQREFVN
                     KGISEGDRQNMAIIRDGMIHMTNLAIYASFSVNGVAALHTEILKAQTFKEFYSLYPEK
                     FNNKTNGITHRRWFVASNPELNKYLTSLIGDSYITNPDDFEKLLPFVKDKKVQRKFLQ
                     IKRLKKQQLAAYIKRANGIKIDVDSIFDVQIKRLHAYKRQLMNVFRIMMLYFRMKSDA
                     DFRIHPVTFIFGAKAAPSYYFAKKVIELINGVADVVNNDPEISPFMKVVFIENYGVKT
                     AEIIIPATDISEQISTAGKEASGTGNMKFMMNGALTLGTLDGANVEIHERVGDEHSFI
                     FGLTEPEVKALRESGTYNPWDVYHNDHEIRRILDSLIDGTWSQGNPHTFKAIFDEVMN
                     KGDEYLILKDLPAYFKRTDEIVTYYRKKSSWAESALYNIAKSGFFSSDRTIHQYIDEI
                     WHIDKVKNGN"
     gene            1373..>1753
                     /locus_tag="PK612_03875"
     CDS             1373..>1753
                     /locus_tag="PK612_03875"
                     /inference="COORDINATES: protein motif:HMM:NF020341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTPase"
                     /protein_id="HOH95278.1"
                     /translation="MEINLAPLLLKQRELDADINSRHNVTHESTKTERFLALLVEIGE
                     LANATRAFKYWSLKKSESRDVLLDEFADGLHFFLSIAIVFNFAVGKVEYESLPATKEE
                     VNNAFKRVYLRITDLMRKESKRCYT"
BASE COUNT          571 a          320 c          359 g          503 t
ORIGIN      
        1 gcctatacaa accatacaat catggtcgaa gcacttgaac gctggccagt gcgttatgtc
       61 catcatttag tgccacgcaa ctacatgatt attgaagaaa ttcatcgtcg tagtcaacgc
      121 gaattcgtta ataaaggcat cagtgaaggt gatcgccaaa atatggcaat tatccgcgac
      181 gggatgattc atatgacaaa cttagcgatt tacgctagtt ttagtgttaa cggtgtcgca
      241 gcgctacaca ctgaaatctt aaaagcgcaa acatttaaag aattctacag tctttatcct
      301 gagaaattta ataataaaac aaacgggatt acgcatcgtc gctggtttgt ggcaagtaac
      361 ccggaactta ataaatattt aacaagtctt attggggact cttatattac taatcccgat
      421 gactttgaaa aactcttacc ttttgtaaaa gataagaaag tgcaacggaa attcttacaa
      481 attaaacgcc tcaaaaagca acaacttgcc gcctatatta agcgcgctaa cggcattaaa
      541 attgatgttg attcgatttt tgatgtgcaa attaaacgtt tacacgctta taaacggcaa
      601 ttaatgaatg tattccgcat tatgatgctt tatttccgga tgaagagtga tgctgatttt
      661 agaattcatc ctgtcacctt tatctttggc gcgaaagccg caccaagtta ctactttgcg
      721 aagaaagtaa ttgaactaat taacggtgtc gctgatgtcg ttaacaatga tccagaaatt
      781 tcgccattta tgaaagttgt ctttattgaa aactatggcg tcaagactgc cgaaattatt
      841 attcctgcga ccgatattag tgaacaaatt agtaccgctg ggaaagaagc aagcggcact
      901 ggtaatatga agtttatgat gaatggtgcc ttaactttag ggacactaga cggcgcgaat
      961 gtggaaattc atgagcgcgt aggggacgaa cattccttta tctttggctt aactgagcca
     1021 gaagtgaaag cacttagaga atcaggtact tataatccgt gggatgttta ccataatgac
     1081 catgaaattc gtcggatatt agattcatta atcgacggta catggagtca aggtaacccc
     1141 catactttca aagcaatatt tgacgaagta atgaataagg gtgacgagta cttaatctta
     1201 aaagatttac cagcctactt taaacgcaca gacgaaattg tcacgtatta tcggaagaaa
     1261 tcaagctggg cggaaagtgc gctctataat attgctaaga gcgggttctt tagcagtgac
     1321 cgcacaattc atcaatatat cgatgaaatt tggcatattg acaaggtaaa aaatggaaat
     1381 taatcttgca ccattactcc ttaaacaacg tgaacttgac gctgatataa atagtcgtca
     1441 taatgtcact cacgaaagta ctaaaacgga gcgcttttta gcgctccttg tggaaatagg
     1501 tgaactcgct aatgcaacgc gtgcctttaa atattggtcg cttaaaaaaa gtgaaagtcg
     1561 cgatgtacta cttgatgaat tcgcggacgg tttacatttt ttcctctcaa ttgcgattgt
     1621 ctttaatttt gcagtcggta aagtagagta cgaaagttta ccggcgacta aagaggaagt
     1681 aaataatgcc tttaagcgtg tttatttacg gattacggac ttaatgcgga aagaaagtaa
     1741 acgctgttac aca
//
LOCUS       DAOSEX010000200         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_70585_length_1730_cov_3.518806, whole genome shotgun sequence.
ACCESSION   DAOSEX010000200 DAOSEX010000000
VERSION     DAOSEX010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70585_length_1730_cov_3.518806"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..845
                     /locus_tag="PK612_03880"
     CDS             45..845
                     /locus_tag="PK612_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95279.1"
                     /translation="MIKRRPEFDPFRDKKVFRCKFCNFPITNPGLYPGKKIMINRYCP
                     ACHSNNTFALLNKQDQTFLRMGFFSNPLYPISPHHLKGVPLKFSLIVFPLSVFFNFVA
                     NIVYMPFGLIYNNIQIKKALKEFYASYKDQDTNLILIDMLEKKAGPKNQYASYKTGIA
                     YLEGIHVPYDLEKAESYFKIAKEEHEGGLVKLAETLIRSENPNYDKAFTILNLVKDNP
                     LASYYLAYLYFYGYGVTPSLRIATIYAGTAHTHNIKDYYDIYPKILQI"
     gene            1000..1161
                     /locus_tag="PK612_03885"
     CDS             1000..1161
                     /locus_tag="PK612_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95280.1"
                     /translation="MEKLVFETIESLLEIKKLRIEERGPVLSRLTDDEIVLLIDNLVE
                     ALQSEYLVQ"
     gene            complement(1208..>1730)
                     /locus_tag="PK612_03890"
     CDS             complement(1208..>1730)
                     /locus_tag="PK612_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOH95281.1"
                     /translation="RFNFHTDIAENILERMRLNELDFAIIDGTSQTDEFNIELLFRDE
                     LIIIGPKDHRLADKKNVSIEELKKEKFILRHEKANTRLAFEKYLSNHFDNINNFDVIL
                     EIDNTAMIKQLIIAGHGLSVMSKAICEINMQVGTLKQIDLRDFHLERGIYLVYPKELE
                     NSEIIDSILALKE"
BASE COUNT          571 a          281 c          292 g          586 t
ORIGIN      
        1 atttaaattt actataatat actaagtgga ggtaagagta acttatgatt aaaagaaggc
       61 ctgaatttga tccgtttcgc gataaaaaag tattccgttg taagttttgt aacttcccaa
      121 ttactaaccc tggactttat cctgggaaga agatcatgat taatcgctat tgtccagcgt
      181 gtcatagcaa taacactttt gcgcttttaa ataaacaaga tcaaacattt ttacggatgg
      241 gtttctttag taacccgctt tatccaatta gcccacatca cttaaaaggc gtgccactga
      301 agtttagttt aatcgtgttc cccttaagtg tgttctttaa ctttgtcgcg aatattgtgt
      361 atatgccgtt tggcttaatt tataacaata ttcaaattaa gaaggcactg aaagagtttt
      421 acgcttctta taaagaccaa gacactaatt taatcttaat tgatatgtta gagaagaaag
      481 ctggtccaaa gaatcagtac gctagttata agacaggtat agcgtactta gaaggcattc
      541 atgttcctta tgacttagaa aaggcggaat cttactttaa aatagcaaaa gaagaacatg
      601 aaggcggctt agttaaactt gcagaaacac tgattcgcag tgaaaaccca aattacgaca
      661 aagcttttac gattcttaat ttagtaaaag ataatccgct agcgagttat tatcttgctt
      721 atctttattt ttatggctac ggtgtaacgc ctagcctccg tattgcgaca atttacgctg
      781 gtactgcgca cacgcataat attaaagatt attacgacat ttatccaaaa atcttacaaa
      841 tctaaaataa aataacatac attctatgtt tgctttacta attgtaaaga cttttttaca
      901 aatttaacac tcctcattac tttggtgagt aagtagtctt atgctataat gcgtttgtag
      961 caaaaaagct tatctagtgg ggagaaggag taaaagagta tggaaaaatt agtgtttgaa
     1021 acaattgaaa gtttattaga aattaaaaag ttacggattg aagaacgtgg tccagtatta
     1081 agtagattaa cggatgatga aatcgtctta ttaatcgaca acttagtgga agccttacaa
     1141 agtgaatact tagtgcaata ataagtattc atttttttag tgaagcacgg tatatggccg
     1201 tgttttttta ttctttaagc gctagaatgc tatcaataat ctcgctattt tctaactctt
     1261 taggataaac aaggtaaatt ccccgttcta agtggaagtc gcgtaaatcg atttgtttaa
     1321 gtgtaccaac ttgcatattt atttcacaaa ttgctttact cattacactt aagccgtgac
     1381 cggcaataat aagttgctta atcattgccg tattatcgat ttctaaaata acatcaaagt
     1441 tattaatatt atcgaagtgg ttacttaaat acttttcaaa cgctaggcgt gtattagctt
     1501 tttcgtgacg aagaataaac ttttcttttt ttaattcttc aatacttaca tttttcttat
     1561 cagcgagccg atgatcttta ggtccaataa taattaactc atcacggaag agcagttcaa
     1621 tattaaattc atcagtctgt gatgtgccgt cgattatcgc aaaatctaat tcatttaagc
     1681 gcattctctc taaaatattt tcggcgatat ccgtatgaaa attaaagcgt
//
LOCUS       DAOSEX010000201         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_71786_length_1711_cov_1.608092, whole genome shotgun sequence.
ACCESSION   DAOSEX010000201 DAOSEX010000000
VERSION     DAOSEX010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71786_length_1711_cov_1.608092"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PK612_03895"
     CDS             <1..1298
                     /locus_tag="PK612_03895"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HOH95282.1"
                     /translation="ENFTAEVKAFHARVRELYPNAYILYEYGHYVNRRFNAEFSATVT
                     ELKTTDPNTGYVYTHLYGAGADSHPSYSQHESIAKELSPQIAEFLGVENPLLPTPVRY
                     EAEEASLTGAATKQSDADLSTPWSNAGYVGDLGPDKAITNPNDILLDGSDVKVLTFNV
                     NAPVAGRYSFTVGYATQVDTASAYVNFDNKGWHKLSLYNTTGWKSMQETAFIELNLSA
                     GNHTVTLTGSCELGSWVNYDYIELGLVDKAGTFLIHTEESNHYSFIDLPTEVIAGEDV
                     SFKLSLHAHYSKSNAVVKVNNEVVTPVEGVYTVTDVQEDLYITVEGIVKNTFVLTFKD
                     GDTIISQTTYEVGATIVVPEDPVKDGYTFNGWDRPIPETMPNGDLTINALWVTGFDEP
                     ETPNNKLKLALGIGIPSITLVGAGLGVGLWFWRKKKLIV"
     gene            1324..>1711
                     /locus_tag="PK612_03900"
     CDS             1324..>1711
                     /locus_tag="PK612_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95283.1"
                     /translation="MKRRISKRWLINGIITTILFGIVMIPVIRDEIKVKTEAATNVPV
                     LAGPSVGWSNSATSNYLFTKEGASSNPEVYKLTFTASDPDVSPSTYQLVKKGTDPWQR
                     FPVVSMIKQIQTLKILLLLFQVVTKTL"
BASE COUNT          565 a          306 c          359 g          481 t
ORIGIN      
        1 ttgaaaactt tacagcagaa gtaaaagcat ttcacgcgcg tgtccgtgaa ctctatccta
       61 acgcttatat tctttatgaa tatggacact atgttaatcg tcgctttaac gcagaattta
      121 gcgctactgt aacagagtta aagacaactg acccgaatac gggttatgta tatacgcatc
      181 tctacggtgc gggcgctgat agtcatcctt catattcaca acacgaatca atcgcgaaag
      241 agttaagtcc gcaaatcgca gaattcttag gtgtagaaaa tccgctttta ccaactccag
      301 tgcgttatga agcagaagag gctagtctta ctggcgcggc cactaaacag agtgacgcag
      361 acttaagtac accgtggagt aacgcgggat acgttggtga cttagggcca gacaaagcaa
      421 ttacaaatcc taatgatatc ttattagacg gaagtgatgt taaagtctta acctttaatg
      481 ttaatgcccc agttgctgga agatatagct ttacagtagg atacgcaact caagttgaca
      541 cagcttcagc ctatgttaat tttgataata aaggctggca taaactttca ctctataaca
      601 caacagggtg gaaatcaatg caagaaacag catttattga acttaatcta agcgctggta
      661 atcacacagt tactttaact gggtcatgcg aacttggcag ttgggtgaat tacgactata
      721 ttgaactcgg actagttgat aaagcgggaa catttttaat tcacacagaa gaaagcaatc
      781 attattcctt tattgattta ccgacggaag ttattgcggg tgaagatgtt tcctttaaat
      841 taagtcttca cgcgcactat agtaaatcaa acgctgtcgt aaaagttaat aacgaagtcg
      901 ttacaccagt agaaggtgtt tatactgtca ctgatgtaca agaagatctc tatatcactg
      961 ttgaaggcat cgttaaaaac acctttgttt taacatttaa agatggtgac actattatta
     1021 gtcaaactac ttacgaagta ggtgctacaa tagtagtacc tgaagatccg gttaaagacg
     1081 gatatacatt taacggttgg gatcgtccaa ttccggaaac aatgccaaac ggtgacttaa
     1141 caatcaatgc cttatgggtg acgggatttg atgaacctga aacaccaaat aataaattaa
     1201 aacttgcttt aggaatcggt attccaagta tcacgcttgt cggcgctggt ctaggagtag
     1261 ggttatggtt ctggcgtaag aaaaagttaa tagtatagct tagtgaaata ggagggcata
     1321 catatgaaaa gaagaattag taaacgatgg ttaattaacg gtattattac cacaatactg
     1381 tttggtattg tcatgatacc tgtaattaga gatgagatta aggtgaagac ggaggctgca
     1441 actaatgtgc cggtactagc aggcccaagt gttggttgga gtaatagtgc aacatctaac
     1501 tacttattta caaaagaagg agcctcatca aacccagaag tatataaact tacatttact
     1561 gcgagtgatc cagatgtatc tccaagcact tatcaacttg taaaaaaggg tactgatcca
     1621 tggcaaagat ttccagtagt gagtatgata aagcaaattc aaacgctaaa gattttgtta
     1681 ttacttttcc aggtggtgac gaaaacttta c
//
LOCUS       DAOSEX010000202         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_72153_length_1705_cov_3.666061, whole genome shotgun sequence.
ACCESSION   DAOSEX010000202 DAOSEX010000000
VERSION     DAOSEX010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72153_length_1705_cov_3.666061"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PK612_03905"
     CDS             complement(<1..902)
                     /locus_tag="PK612_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOH95284.1"
                     /translation="MLNVKALYTAYEKGELKAIPVKLQGWVKTNRNNGTIGFIEFNDG
                     SAFKGVQIVYEKNKIDNFAEVDKYRTGAALEVYGKVVLTPEMKQPFEVHADKVVLVGD
                     VAEDYPLQKKRHTPEFLREIAHLRPRTNTHTALFRLRSELSYALHKYLYDHEFVYVHT
                     PIITGNDAEGAGNVFNVVTDHKDPNFFFGKEASLTVSGQLHVESFALTFGKVYTFGPT
                     FRAEESNTTRHAAEFWMLEPELAFTDLNGNMDSIEEIIKYSVAYVLEKCPDEIDFFTK
                     FYDKTLKDRLEALVKSKVLRITYT"
     gene            complement(938..>1705)
                     /gene="tsaD"
                     /locus_tag="PK612_03910"
     CDS             complement(938..>1705)
                     /gene="tsaD"
                     /locus_tag="PK612_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOH95285.1"
                     /translation="GPGLIGALHVGLQAAKTLALLYNKPLIPVHHLAAHIYASEFVKP
                     LVFPLLAIVVSGGNTEFVVMKKHLDFTTIGETRDDAIGECYDKIARVLNLGYPGGIPI
                     DRLAKEGEATYKLPSPALTGYDFSFSGLKTHVINLVNNTKQRGEEINVPNLCASFQKV
                     AIDAIIKKGLKAAQEYKVKQVVIAGGVSANSYLRSEVLAKFPKIGVDPAIPPLWCCTD
                     QAAMIAKLASYLYDEKVFADYDLGVDPNWEIEDFREF"
BASE COUNT          507 a          348 c          287 g          563 t
ORIGIN      
        1 ttagtgtatg taatacgtaa tactttactt ttcactaatg cttctaaacg gtcttttaat
       61 gtcttatcat aaaatttcgt aaagaaatca atttcatcgg gacatttttc taatacataa
      121 gcaacagaat acttaataat ttcttcaatt gaatccatat taccatttaa atcggtgaac
      181 gctagttcgg gttcaagcat ccaaaactcc gctgcgtgcc gggtagtatt actttcttca
      241 gcacggaatg ttggtccaaa ggtataaact ttaccaaatg ttaacgcaaa tgattcaaca
      301 tggagttggc ctgagacagt aagtgaagct tctttaccaa aaaagaagtt tggatcttta
      361 tgatcagtaa caacattaaa gacattaccc gctccttcag cgtcgtttcc ggtaataatt
      421 ggtgtatgaa catagacaaa ttcatggtcg tataagtatt tatgaagcgc atatgatagt
      481 tcactccgga gtcggaataa agcagtgtgt gtattggtcc gcggtcttaa gtgcgcaatc
      541 tctcttaaaa attcaggagt atgccgtttc ttttgtaacg gataatcttc tgcaacatca
      601 ccaacaagaa caactttatc agcgtgtact tcaaaaggtt gcttcatttc tggagttaac
      661 acaacttttc cgtaaacttc aagcgccgct ccagtgcgat atttatcaac ttcagcaaag
      721 ttatcaatct tatttttttc ataaactatt tgtacaccct taaaggcact accatcatta
      781 aattcaataa aaccaatcgt gccattatta cgatttgttt tgacccatcc ttgtaattta
      841 acaggaatcg ctttaagttc gcctttttca taggcggtat atagtgcttt tacatttaac
      901 atattgttac ctctttgact tctttattaa ttatatctta aaactctcta aaatcttcaa
      961 tttcccaatt aggatcaaca cctaaatcat aatccgcaaa aactttttca tcatataagt
     1021 atgatgctag cttagcaatc atcgctgctt ggtcagtaca acaccatagt ggcggtattg
     1081 cgggatcaac tccaattttc gggaattttg ctagtacttc gctccgtaaa tatgagtttg
     1141 cgctcacgcc accagcaatt acaacttgtt tgactttata ttcttgtgct gcttttaatc
     1201 cttttttaat aatggcatca attgcgactt tttgaaatga tgcacataag ttcggcacat
     1261 ttatttcttc gcctctttgt ttagtattat taactaaatt aattacatgt gtctttaacc
     1321 cggaaaaaga aaagtcgtaa ccagttaagg caggacttgg taatttgtaa gttgcttcac
     1381 cttcttttgc taaacggtca attgggatac cgcccggata acctaaattt aagacacgcg
     1441 ctattttgtc atagcactct ccaatcgcgt cgtcacgcgt ttccccaatt gttgtaaagt
     1501 ctaaatgttt tttcatcaca acaaattcag tgttaccgcc acttacaaca atagcaagca
     1561 gtggaaacac aagcggttta acaaattcac tagcgtaaat atgcgctgct aagtggtgaa
     1621 cagggatcag cggtttgttg taaaggagcg ctaatgtttt agcagcttgt aacccaacgt
     1681 ggagcgcccc aataagtcca ggtcc
//
LOCUS       DAOSEX010000203         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_73385_length_1686_cov_3.140405, whole genome shotgun sequence.
ACCESSION   DAOSEX010000203 DAOSEX010000000
VERSION     DAOSEX010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73385_length_1686_cov_3.140405"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PK612_03915"
     CDS             <1..818
                     /locus_tag="PK612_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH95286.1"
                     /translation="YFFYFIIPFIALLIGAISGVIFSALHAFASITMKSDQTISGTAL
                     NMLAAGLAIFMGRALSLGRTEEITFSSTHHRISAIPLLRHIPFFGKILFENIYLISLF
                     GILLFIVMGVIAYKTKFGLRLRACGENPHAADAAGINVIRTRYIAVLISGMLGGIGGV
                     LLIIPTTVSFIANVYGFGFLALAVLISGQWKPSRIMIFAIFFGLLRSIAFGFAFIKAD
                     LSHIAFLKTFLDKLPTDILNMLPYVVTLIVLAFTSLKSRGPKAAGEPYDQGKR"
     gene            898..974
                     /locus_tag="PK612_03920"
     tRNA            898..974
                     /locus_tag="PK612_03920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Arg,seq:acg)
     gene            1165..>1686
                     /locus_tag="PK612_03925"
     CDS             1165..>1686
                     /locus_tag="PK612_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HOH95287.1"
                     /translation="MSKINEIQVLLSKKAELNARLSLLPYDGTPEIKTISGKDYLYVR
                     KRVLNKVTSTYVDVYSDKLYNNLLRNNEEAKKLRREIRKIDKALASFGYKEGILLPNV
                     ILARDFARKNRNAIIYDQVILEGIGTTFPETEAILEQGIIKNARAEDVQKILNLKRAW
                     EFILDDNVLLAKTD"
BASE COUNT          522 a          281 c          331 g          552 t
ORIGIN      
        1 agtatttttt ctattttatt ataccattta ttgctctttt aattggggct atttctggcg
       61 ttatattctc cgccttacac gcttttgcct caattacaat gaaaagtgat caaacaattt
      121 cggggacagc acttaatatg ctcgctgctg ggttagcaat ctttatgggc cgcgcacttt
      181 cactaggccg aacagaagaa attacctttt caagtacgca tcatcgtatt agtgcgattc
      241 cgcttttacg ccatattccg ttctttggta aaatcctgtt tgaaaatatt tacttaattt
      301 cattattcgg tattttgctc tttattgtga tgggtgttat tgcctataag acgaagtttg
      361 gattacgctt acgagcatgt ggcgaaaatc cgcatgctgc agatgcagcc ggaattaatg
      421 tcatcagaac tcgttatatt gctgttttaa tcagcggaat gctcggcggt attggcggtg
      481 tcttacttat tatcccgacg accgtgagtt ttatcgcgaa tgtgtatgga tttgggttct
      541 tagcacttgc cgttcttatc agtggtcaat ggaaaccaag tcggattatg atttttgcga
      601 ttttctttgg actcttacgg agtattgctt ttggttttgc ttttattaag gcagacttaa
      661 gtcatattgc gttccttaaa acattcttag ataagttacc gacggatatt ttaaatatgt
      721 tgccatatgt tgtaacactt attgtgcttg cctttacatc acttaaatca cgcggaccaa
      781 aagccgcagg tgaaccttat gaccaaggga aacgctgatt atttgctaaa tattacatat
      841 ttaactttat aacacttgca aaaatcactt aaataactat aatgtttaag tgacgctgcg
      901 gtcatagctc aactggatag agtgccagac tacgaatctg gaggttggga gttcgattct
      961 tcctgaccgc gccattgcga ataaacatgc tccctaaaat tgcactagag tctagtacaa
     1021 tgaaaagggg agctttttat ttatgaaatc taattatgtc taatacatgc gataaacgaa
     1081 taaatttgac aaagtaaaat ttagatttgt tttagggaac agattgtgta taattaagtt
     1141 agaaagttcc ctaagagaat tgttatgagt aaaataaatg aaatacaagt attattatca
     1201 aaaaaagctg aactaaacgc acggctatcg ttattgccgt atgatggcac tcctgaaata
     1261 aaaacaatca gcggcaaaga ttatctctat gtgcgaaaaa gagttctaaa taaagtgact
     1321 tcaacctatg ttgatgttta tagtgacaag ctttataaca atctacttcg taataatgaa
     1381 gaagcaaaaa aacttaggcg tgaaatacgc aaaatcgata aagctttagc gagttttggt
     1441 tacaaagaag gaatactatt acctaatgtc attttagcgc gggactttgc gcgaaaaaat
     1501 cggaatgcga ttatttatga tcaagttata ttagagggaa ttggtacaac ttttcccgag
     1561 acagaagcaa ttctcgaaca aggtattatc aagaatgcta gagcggaaga tgtacaaaaa
     1621 attcttaatt tgaaaagagc gtgggaattt attctagatg ataatgtttt attagcgaaa
     1681 accgat
//
LOCUS       DAOSEX010000204         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_73803_length_1680_cov_2.798769, whole genome shotgun sequence.
ACCESSION   DAOSEX010000204 DAOSEX010000000
VERSION     DAOSEX010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73803_length_1680_cov_2.798769"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="PK612_03930"
     CDS             <1..1119
                     /locus_tag="PK612_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOH95288.1"
                     /translation="HKLPQSKVLMNLDDDLVNRTAIKAVEKLSPFLTDEKAEDTVLSP
                     ATYSLAMAGLSAVSDNFNNDAFGLINAEEDVKALLEAWNFKFKNYTGETFFRSAVLHQ
                     QVGPTYAFDKSKQEAVSDHYISTMVSSHESYRKDADKFFDKKIGLKLKVPDLNLKSDG
                     VVTYGVLKMKDYVANDGLFHRHMPFTFKSGLGTADSYVFAVREDPKKVAYYRGGNYEV
                     FKVNIRNTDLMIVLPNEEVNINHVNITEAYTNFVNEAELVPAFGYVPFFHNKVEGLNL
                     TDTLENVITHNEIYMSKLLKDDVYNDLVIDAVVQSNDFEFNKYGVAGESMTAVSGCTS
                     TGPLYDEPIDLSVNRPFYAFSLKDKYPIFINKVSKPGA"
     gene            1343..>1680
                     /locus_tag="PK612_03935"
     CDS             1343..>1680
                     /locus_tag="PK612_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH95289.1"
                     /translation="MIVLNLKLDNILAFKDFEVNFSYPRKLKKTLIEGEHLRTIPTFR
                     YKKLVVLIGSNATGKTSLIKLIWRISLFLHRKNPSFLDSLISENNEHGNIEIDFVNEY
                     DDVAILYRAK"
BASE COUNT          579 a          243 c          285 g          573 t
ORIGIN      
        1 cacaaattac cacagtctaa agtgctgatg aatttagatg atgacttagt aaatagaaca
       61 gcaattaagg cggttgagaa attatccccc tttcttactg atgaaaaagc agaagatact
      121 gtgctatcac cagcaactta ctccttagcg atggctgggc tttccgctgt tagtgataac
      181 tttaataatg atgcttttgg tttaattaat gccgaagaag atgtaaaagc gctcttagaa
      241 gcatggaatt ttaaatttaa aaattacaca ggtgaaactt ttttccgctc ggcagtctta
      301 catcaacaag ttggaccaac ttatgctttt gataagagta aacaagaagc agtaagtgat
      361 cattacattt cgacaatggt ctcttcgcac gaatcttacc ggaaagacgc tgataaattt
      421 tttgataaga agattggttt aaagcttaaa gttcctgatt taaatcttaa aagtgatgga
      481 gtagtgactt acggtgtttt aaaaatgaaa gattatgtcg ctaacgatgg gctttttcat
      541 cgacatatgc cttttacatt taaaagcggg ctaggcacag cggattccta tgtctttgct
      601 gtaagagaag atccaaagaa agttgcttat tatcgcggag gtaattatga ggtatttaaa
      661 gtaaatattc gaaatactga tttaatgata gttttaccaa acgaagaggt taatattaat
      721 catgtaaata tcactgaagc ttacactaat tttgttaatg aggctgaact tgttcccgct
      781 tttggctatg tacccttctt ccataacaaa gttgaaggtc taaatttaac agacacatta
      841 gaaaatgtta ttacgcataa cgaaatctat atgagtaaat tacttaaaga tgatgtttat
      901 aatgatttag ttattgacgc tgttgtgcaa tcaaatgact ttgaatttaa taaatatggt
      961 gtggcaggag aatcaatgac cgcagtttcc ggctgtacat caactggtcc actttacgat
     1021 gaaccaattg atctttctgt taatcgtccg ttttatgcct tctctttaaa agataaatac
     1081 ccaatcttta ttaataaagt gtcaaagcct ggcgcataat tattgtttgt cataaaacta
     1141 tttactaaaa ataaggctac atcaaaatgt agtctttttt taaaaattga ttttaattta
     1201 ttggtcgctg catattctat ttaagtattt aacatttttt taaaaagttt taaaaagttt
     1261 gaaaatactt gtttttcaaa ttaaaattta ttacaataaa attgtaagtt caaaaaactt
     1321 gaatatacaa ggagcaaata atatgattgt cttaaatttg aagttagata atattctagc
     1381 ctttaaggat ttcgaagtta attttagtta tccgcgtaaa cttaagaaaa cgctaattga
     1441 aggagaacat cttcgcacaa ttccaacttt tagatataag aaattagtag tgttaattgg
     1501 ttcaaacgca acagggaaaa cttctctcat taaattaatt tggagaattt ctttatttct
     1561 gcatcgtaaa aatcctagtt ttttagatag tcttataagt gaaaacaatg aacacggcaa
     1621 cattgaaatt gactttgtaa atgaatatga tgatgttgct attttatacc gcgctaaaat
//
LOCUS       DAOSEX010000205         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_74231_length_1674_cov_3.432983, whole genome shotgun sequence.
ACCESSION   DAOSEX010000205 DAOSEX010000000
VERSION     DAOSEX010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74231_length_1674_cov_3.432983"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1674)
                     /locus_tag="PK612_03940"
     CDS             complement(<1..>1674)
                     /locus_tag="PK612_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOH95290.1"
                     /translation="IIQTLEQLNSCRENGLTTEEALKRQEVHGKNELPKAKKKTIVGV
                     FFSQMNNAMIYILLVAAVISFVMALIEGDGSFFEPILILAIVLLNAVIGAIQELKADK
                     ALEALEKLSAPTTVVRRDGELKEIKASELVPGDIVIIEDGRTIPADLRLISSANLKVS
                     EASLTGESVPVEKDANIVYDRPMPIGDRKNVVYMSTPVVYGRGEGVVIGTGLQTEIGQ
                     IAKMLQTTKQEETPLQKRLADLSKLLGIITFVLVALLFIIALIKDPTWDNIKNMFLLS
                     ISLAVAAVPEGLPAVVTITLALGVQRMVKVHTITRRLPSVETLGAVSVICSDKTGTLT
                     QNVMTVVSAYTNEKQYEVEDFSADTVGELADGLSLCSDASVDHGVYGDPTEIALVNFA
                     NLIDHHKDDLEAATPRVEELPFDSVRKMMSTLHKTGKTYTQYTKGAMDNVLKLTTHIS
                     VNGKVREIKKSDITAINDVASKMAKRALRVLALAKKEVPFAGISEENLVFVGLVGMVD
                     PPRPEAIPAVENLKGAGITTIMITGDHADTAYAIAHELGITDSPSNVLTG"
BASE COUNT          495 a          374 c          274 g          531 t
ORIGIN      
        1 ccagttaaaa cattacttgg gctatcagta ataccaagtt catgggcaat cgcgtaagct
       61 gtatcagcgt gatcgccagt aatcataatt gtcgtaatac ctgcaccctt taaattttcc
      121 acagcaggaa ttgcttctgg acgcggtgga tcaaccattc ctaccaagcc tacaaaaaca
      181 aggttttctt cacttatacc ggcaaaaggc acttctttct tcgctaaagc taacacacga
      241 agtgcacgtt tagccatttt actagcgaca tcattaattg ccgtaatatc acttttttta
      301 atttcacgaa ctttaccatt aacactaata tgggtcgtaa gttttaaaac attatccatt
      361 gccccttttg tgtattgggt atatgtttta ccagttttat gtaatgttga catcatcttt
      421 cttacactat caaatggtaa ttcttcaaca cgcggtgttg ccgcttctaa atcatcttta
      481 tgatggtcaa tcagattagc aaaattgaca agcgcaattt ccgttgggtc gccataaact
      541 ccgtgatcaa cacttgcgtc actacaaaga ctaagaccat cggctagttc accgacagtg
      601 tcagcactaa agtcttccac ttcatactgt ttctcattag tgtatgcact aacaacagtc
      661 atcacatttt gtgttaaagt tccagtttta tcactacaaa taacactgac ggcaccaagc
      721 gtttcaacgc tcggaagtct tctggtaatt gtatgaactt taaccatccg ttgcacacct
      781 aacgctagtg taattgtcac aaccgctggt aagccttctg ggaccgccgc tactgctaaa
      841 gaaatagaaa gcaggaacat atttttaata ttgtcccaag ttggatcttt aataagcgca
      901 ataataaata aaagcgcgac taatacaaaa gttataatcc ctaataattt tgataagtca
      961 gcaagccttt tttgtaatgg tgtttcttct tgcttcgttg tttggagcat cttcgcaatt
     1021 tgaccgattt cagtttgtaa accagtacca attacaaccc cttcaccgcg accatagaca
     1081 acaggagtcg acatataaac aacattttta cgatcaccaa ttggcattgg acggtcataa
     1141 acaatgtttg catctttttc aacaggaaca ctttcacctg taagactagc ttcgctaacc
     1201 ttaaggtttg cgctactaat taagcgtaag tccgctggta tcgtacgacc atcttcaata
     1261 ataacgatgt cacctggaac aagttcacta gccttaattt ctttaagttc accatcccgt
     1321 cgtacaactg ttgtaggggc actaagtttt tctagtgctt caagtgcttt atccgctttt
     1381 aactcttgaa tagctccaat tactgcgtta agtaatacaa ttgctaaaat taaaataggt
     1441 tcaaaaaagg aaccatcccc ttcaattagc gccatcacaa aagaaataac agcagcgaca
     1501 agtaatatat aaatcatcgc attattcatc tgcgagaaaa agacaccaac aattgttttc
     1561 tttttcgctt ttggtaattc gtttttcccg tgcacttctt gacgtttaag tgcttcttcc
     1621 gtcgttaaac cattttcgcg acacgaattt agttgttcga gtgtttgtat aatt
//
LOCUS       DAOSEX010000206         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_74623_length_1668_cov_4.212647, whole genome shotgun sequence.
ACCESSION   DAOSEX010000206 DAOSEX010000000
VERSION     DAOSEX010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74623_length_1668_cov_4.212647"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PK612_03945"
     CDS             <1..635
                     /locus_tag="PK612_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95291.1"
                     /translation="EYRSRTEGVTFAITKYDGRIIEEVPSNRFFTEVNVGLKGYFPKA
                     TSKIINFLLVDKTVALPVVKVDDVRIFTPEQVQDFNGYKINFELYHDLFVPAKLQSAV
                     YAHVGTAAATSKMARLNVALAAGASGKSKVTGYATLPLGLSGALYGIAGDTGFVVGEA
                     IADYSGAVAITVGTDFTAFDGDKGYFCLVKDGKVIAVSAKYTSIPKGSGS"
     gene            726..1643
                     /locus_tag="PK612_03950"
     CDS             726..1643
                     /locus_tag="PK612_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95292.1"
                     /translation="MEFKTRYITADEFKQYFGIDLQIELPNGDNESGKVAAYLTRIEN
                     RLEAWINANYFYNVTQFWETLNNNQKEQYKYALLEQAYYVLRMGDISVDSGYDKERGI
                     IAGRSTINELSVAPNAMLHLENGGFLNTRIVKRGGYYQGVVANSQVVAGGVVGQKGEP
                     GKDGLTPFIGNNGNWWIGGLDTGVTAAGQPGTNGTNGSGFGETFRGNDLFLAIRLDDG
                     NVIKNYLIAFDTVSATGQATTYIDDTDTRETISLADSIGKILSIHYIGGLPVSTVHYY
                     LNGVKIKTYECYDIELYFGSENAKLVEIL"
BASE COUNT          530 a          278 c          387 g          473 t
ORIGIN      
        1 atgaatatcg tagccgcacc gaaggtgtta cctttgcgat tacgaaatat gatggtcgga
       61 ttatcgagga agttccatca aaccgcttct ttactgaagt caatgttgga ttaaaaggat
      121 actttccgaa agcgacatca aagattatta acttcttatt agttgataaa actgttgctt
      181 taccggttgt taaggttgat gatgttcgca ttttcacacc agaacaagtg caagatttca
      241 atggttataa aatcaacttt gagctatacc acgacttatt cgttcctgcg aaattacaaa
      301 gtgcggtcta cgctcatgtt ggcacagcag ctgcaacgag taaaatggca agacttaatg
      361 tcgcattagc tgctggagca tccggcaaat cgaaagttac tggttacgca accttaccgc
      421 tcggattaag cggcgcgtta tatggaatcg cgggagatac cggattcgtg gttggagagg
      481 caatcgctga ttacagtggc gcagtcgcca ttacggttgg aactgacttc accgcatttg
      541 atggcgataa aggctacttc tgtctcgtaa aagatggcaa agtcatcgct gtcagtgcta
      601 aatacacatc aattcctaaa ggttctggaa gttaattact aacaatttag taaaaaacgg
      661 ggagagtgtt tatcgctctc ctttttttat gataaaataa gtatatagac aattaaggag
      721 ttattatgga attcaaaaca agatatataa cagcagatga atttaagcag tattttggaa
      781 tcgacttaca aattgaattg ccaaacggcg acaatgaaag tgggaaagta gcggcatatc
      841 tcactcgaat tgagaatcga cttgaagcgt ggatcaacgc aaattatttc tacaatgtca
      901 cgcagttttg ggaaacatta aacaataacc aaaaagagca atacaaatac gctttactcg
      961 aacaagcata ttatgtttta cgaatggggg acatttcagt tgatagtggc tatgataaag
     1021 aacggggaat tatcgcgggt cgttcaacga taaacgaatt gtcagtcgca cctaatgcta
     1081 tgcttcattt agaaaacggt ggtttcttaa acactcgcat tgtcaagcgt ggtggttatt
     1141 atcaaggtgt cgtagcgaat tcacaagtag tcgctggtgg tgttgtcgga caaaaaggcg
     1201 aaccgggaaa agatggctta acgccattca tcggtaataa tggtaattgg tggattggtg
     1261 ggttagatac gggagtaacg gctgccggac aaccgggaac gaatggaacg aatggtagtg
     1321 ggtttggtga aacttttaga ggtaatgatt tgtttttagc tatcagactc gatgatggaa
     1381 atgtgattaa aaattactta atcgcttttg ataccgtatc ggcaacggga caagcgacta
     1441 catatataga tgatacagac acgcgagaaa ctatttcatt agccgatagt attggtaaaa
     1501 tattatctat tcactatatt ggtgggttgc cagtgtcaac ggttcattac tacttaaacg
     1561 gagttaagat taaaacttat gagtgctacg atatagaatt atattttggg agtgagaacg
     1621 ctaaactcgt ggagatttta tgataagcaa aagaagttat aacgagat
//
LOCUS       DAOSEX010000207         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_74635_length_1668_cov_3.347799, whole genome shotgun sequence.
ACCESSION   DAOSEX010000207 DAOSEX010000000
VERSION     DAOSEX010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74635_length_1668_cov_3.347799"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1668)
                     /locus_tag="PK612_03955"
     CDS             complement(<1..>1668)
                     /locus_tag="PK612_03955"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOH95293.1"
                     /translation="EFPLWDSNTFLGVNNIGSNSFYYSMNPFFLPILIFPRELIPQGI
                     SILMIGKFVLAAVTMRLYLKYMGVNEKEARFFAVIYAFSGWNTYYLWFNHFMEVAVIF
                     PLIFLGIEKLIKEKVMWVLILSLGLMGFANYFFLMTVGVIGVLYAGFRYFQQLPKVEP
                     SIIGLGIIAFALGLLLSSVVLFPSLVIALGSDRATDATYLEKLLDLFKNVNSISLKEQ
                     RRQIWHLITKWEPYTMEGATPSTQYSYKKFYPLISYYFPVLSNRSVALLNTSNYDNTI
                     SSLFSFSPVIILFIPSLVVSAKKKKVSHFVALAFFLFALFTPFFYHAFHGFTKDYGRW
                     QLIVTFSLITYVAMSLPEVKAEKPYLLDISIIFNLIMMGITAKVAFSFENKSGFSYMY
                     QREYVIYYQAIMLFISYLVLRLARNTKHEYYLRNAFVFFEIAVMGTITMIGHGFISYK
                     DSVSGGYQNYKDDLTTIREIKALDGSYYRIFNTRAYRGNDNLPMRENYNGLSAFHSLY
                     NFELMPFNKWSKINYNHKGWSLGVFEKRSMLNDFLQVKYYMLNNTNHV"
BASE COUNT          605 a          304 c          262 g          497 t
ORIGIN      
        1 aacatggttc gtattattaa gcatatagta cttaacttgt aagaagtcat ttagcatact
       61 acgtttttca aagacaccta aactccagcc cttatggtta tagttaattt ttgaccattt
      121 gttaaatggc atcagttcaa agttataaag actatggaag gcacttaacc cgttataatt
      181 ctcacgcatt ggtaaattat cattaccacg ataggcacga gtgttaaaaa ttcggtaata
      241 agaaccatct aaggctttaa tttctctaat cgttgttaaa tcatctttat aattttggta
      301 accacctgac acactatctt tatagctaat aaagccgtgc ccaatcattg ttatcgtccc
      361 cattaccgct atttcaaaaa acacaaaagc attgcgtaaa taatattcgt gttttgtgtt
      421 acgcgctaaa cgaaggacta aataactaat aaaaagcatt attgcttggt agtaaattac
      481 atattccctt tgatacatat aactaaagcc gctcttattt tcaaaagaga acgctacttt
      541 agcggttatt cccatcataa ttaagttaaa gataatactt atatctaaaa gataaggctt
      601 ttctgccttc acttcaggaa gcgacattgc aacataagtt attaaactaa aagttacaat
      661 tagttgccat ctaccataat ccttggtaaa cccgtggaaa gcgtgataga agaagggcgt
      721 aaataaagca aacaagaaaa acgcaagtgc tacaaagtga ctaacctttt tcttttttgc
      781 ggatacgact aatgaaggga taaataaaat aataactggg ctaaacgaaa acaaacttga
      841 aatcgtgtta tcgtaatttg atgtattaag tagagcgaca ctcctattac ttaatactgg
      901 gaagtaataa gaaataagcg gataaaactt tttataacta tactgtgtac tcggagtagc
      961 gccttccatg gtgtaaggct cccatttagt aattagatgc caaatttggc ggcgctgttc
     1021 tttaagactg atactattaa catttttaaa taagtctagt aatttttcta aataagtggc
     1081 gtctgtcgct cggtcactac ctaaagcaat gactaaactc gggaataaca ctacactact
     1141 taatagtaat cctaaagcaa aagcaataat acctaatcca ataatacttg gttcaacctt
     1201 tggtaattgt tgaaagtagc gaaaaccagc gtagagcacc ccaatgaccc caacagtcat
     1261 taagaagaag taattagcaa aacccattaa gcctaatgac aaaattagga cccacataac
     1321 cttttcttta attaattttt caataccaag gaaaataagc gggaaaataa cggcaacttc
     1381 catgaagtgg ttaaaccata agtaatatgt attccagcca ctaaatgcat agattacagc
     1441 aaagaatcgt gcttcttttt cattaacgcc catatatttt aaatataaac gcattgttac
     1501 tgccgctagt acaaacttac caatcattaa aatactaatc ccttgtggga taagttcacg
     1561 cgggaaaata agaattggta agaagaaggg attcattgaa tagtaaaaac tatttgaacc
     1621 aatgttatta acacctaaaa aggtattact atcccacagc ggaaattc
//
LOCUS       DAOSEX010000208         1655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_75607_length_1655_cov_2.405625, whole genome shotgun sequence.
ACCESSION   DAOSEX010000208 DAOSEX010000000
VERSION     DAOSEX010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75607_length_1655_cov_2.405625"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1655
                     /locus_tag="PK612_03960"
     CDS             <1..>1655
                     /locus_tag="PK612_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH95294.1"
                     /translation="EKDFVFVQQDEKIHDLKFETKPTTYFKDAMRRFVKNKSSVVAGV
                     IIGILILLALFVPIFNKNDIVTSQEDYRFLPPRWPGFQNTSVLNGRRYVENIIYDATD
                     EDETKHHPVGYDNEAIVGEVKLTEGTTNFASPYASGGKVLISSDRSSFATGIQTNKIT
                     ADFALNDYYLEIVTDTELNRDNNYEIYVLVYYNGPNSDATLVVLQEQSMKYGTFTTNL
                     SDVVLSSRPAAILEDEFTVQIGVYVPATNSPDGTRAQLYLNKLAVTTTAEENPFELHN
                     YTDANELLLRRSDKEKIPYRYQTVISGNQMLYEGRVIRASFEYDYYKAVYGEVNRIIG
                     ISDLLNARRDGKVKFDLDPAHSDHTKPDFNYQIVMDTFEVVNDKTSQYREITFAHPRQ
                     TVKAGMTTIYVYNYDVVVSRYREMGYTREPFFLFGTNQRGQDFFKIVFNGLRTSLILG
                     FSAAAINIFIGLVWGAISGYYGGTVDILMERFTEILGGVPWIIIMTLTILHLGNNFGV
                     FLLALILTGWIGTASVTRSQFYRYKRREYVLASRTLGAKDSRL"
BASE COUNT          518 a          286 c          326 g          525 t
ORIGIN      
        1 tgaaaaagat ttcgtgtttg ttcaacaaga cgaaaagatt catgacttaa aatttgaaac
       61 taaaccgaca acatatttta aagacgcaat gaggcgtttt gttaaaaaca aatcaagtgt
      121 tgtcgctggt gtgattattg gcattttaat tcttttagcg ttatttgtgc caatctttaa
      181 taaaaacgat attgttactt cgcaagaaga ttatcgcttc ttaccaccaa ggtggcctgg
      241 ttttcaaaat acttcagtat taaacggtcg ccgctatgtt gaaaacatca tttatgatgc
      301 gactgatgaa gatgaaacta aacatcatcc agtaggttac gataacgaag caattgttgg
      361 tgaagtaaaa ttaaccgagg gtaccactaa cttcgcttcg ccatacgcat caggcggaaa
      421 agtgcttatt tctagtgata gaagcagttt cgctactggt attcaaacga ataaaattac
      481 tgcagacttt gctcttaatg attattattt agagattgta actgatactg aacttaatcg
      541 tgataataac tacgaaattt atgtgcttgt gtattataac ggtcctaaca gtgatgcgac
      601 actagtcgta ttacaagaac aaagtatgaa atatggtact tttactacta atttaagtga
      661 tgtagtatta tctagtcgtc ctgcagcgat attagaagat gaattcactg tacaaattgg
      721 tgtttatgtg cccgcaacta atagtccaga tggtacgcgc gcgcaattat atttaaataa
      781 attagcggta acgactactg ctgaagaaaa tccttttgaa ctacataact acactgatgc
      841 gaatgaatta ttactacgcc gaagtgataa agaaaaaata ccatatcgct atcaaacagt
      901 tatttctggt aatcaaatgc tctatgaagg tcgtgtcatt cgcgctagtt ttgaatacga
      961 ttactataaa gcagtttatg gtgaagttaa tcgtatcatt gggattagtg atttactaaa
     1021 tgcccgtcgt gatggcaaag ttaaatttga ccttgatccg gcccatagcg atcatacaaa
     1081 accagatttt aactatcaaa tcgtgatgga tacatttgaa gttgtaaacg acaaaacatc
     1141 acaatatcgc gaaattactt ttgcccatcc acggcaaaca gttaaagctg ggatgacaac
     1201 aatttatgta tataactatg atgtcgttgt ttctcgttat cgggaaatgg gttacacacg
     1261 tgaaccattc ttcctttttg gcacgaatca acgtggtcaa gatttcttta agattgtctt
     1321 taacggttta cgcacatcct taattttagg ctttagtgct gccgctatta atatttttat
     1381 cggtttagtc tggggtgcca tcagtggtta ctacggcgga acagttgata ttttaatgga
     1441 acgctttacg gaaatcttag gtggggtacc gtggatcatt attatgacct taacaatctt
     1501 acacttaggg aataactttg gcgtcttcct tttagcgctt attctcacag gctggatcgg
     1561 cacggcaagt gtaacgcgta gtcaattcta tcgttataaa cgccgagaat atgttttagc
     1621 aagtaggaca cttggggcga aagatagtcg cttaa
//
LOCUS       DAOSEX010000209         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_75877_length_1651_cov_2.909148, whole genome shotgun sequence.
ACCESSION   DAOSEX010000209 DAOSEX010000000
VERSION     DAOSEX010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75877_length_1651_cov_2.909148"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /gene="sufB"
                     /locus_tag="PK612_03965"
     CDS             complement(<1..1324)
                     /gene="sufB"
                     /locus_tag="PK612_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012243143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOH95295.1"
                     /translation="MTSADELKFGTYAYGFSDDQESIASTGIGINEDVVRQISAFKEE
                     PEWMLEFRLKSLRAFELMKNPKFGPSLKDLDFDSYTYFNRVVKDEARSWDDVPDTIKE
                     TFEKLGIPEAERKFLAGVATQYDSEVVYASMISEFKEKGVIFSSTDEALKEHPEIFKK
                     YFNTVVKYTDNKYTALNGAVWSGGSFIYVPKGVELDKPLQSYFRLNAAKMGQFERTLI
                     IVDEGASVHYVEGCTAPVYSEDSLHAAVVEIIVHKDAKCRYTTIQNWSNNIINLVTKR
                     ADVYENGQMEWIDGNIGSSINMKYPACILRGEGARGTTISIAVAGKGQYQDAGARMIH
                     LAPNTSSTIISKSVVHSGGTANYRGLVRHTKNAPNSQTHIECDTLILDGISKSDTIPT
                     NEIHNNESFIEHEATVSKISEEQLFYLMSRGLSEEDATQMIIMGFIEPF"
     gene            complement(1317..>1651)
                     /locus_tag="PK612_03970"
     CDS             complement(1317..>1651)
                     /locus_tag="PK612_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOH95296.1"
                     /translation="CIDNLTLYLKVKDNKVITGYFDGFGCTISIASTSILLEMIEGMN
                     VDAVLKIIAEYKKMLNSEPYDIDILNEAIVFANTVRQPSRIRCATIGWDGIAELITKH
                     KEEENHND"
BASE COUNT          498 a          339 c          258 g          556 t
ORIGIN      
        1 agaatggttc aataaagccc ataataatca tttgcgttgc gtcttcttca cttaaaccgc
       61 gactcattaa ataaaataac tgttcttcac taattttact taccgttgct tcgtgttcaa
      121 taaatgactc gttgttatgt atttcattag taggaattgt gtcacttttt gatatcccgt
      181 ctagtattaa agtgtcacat tcaatatgtg tttgtgaatt aggagcattt tttgtatgtc
      241 gcactaaacc gcgataattt gccgtgccgc cactatgtac gactgattta gaaataatgg
      301 tacttgatgt atttggtgct aagtgaatca ttcttgcacc tgcatcttga tattgacctt
      361 tccctgcaac agcaatcgaa attgttgtgc cgcgtgctcc ttcaccgcgt aagatacaag
      421 caggatactt catattaata gagctaccaa tattcccatc aatccattcc atctgtccgt
      481 tttcataaac atctgctctt tttgtaacga gattaataat gttatttgac cagttttgaa
      541 tcgtagtgta gcgacattta gcgtctttat gtacaataat ttcaacaaca gcagcgtgta
      601 atgagtcttc actataaact ggagcagtac aaccctcaac ataatgaaca cttgcccctt
      661 catcgacaat aattaatgtt ctttcaaatt gacccatctt tgccgcatta agtctaaagt
      721 aactttggag tggtttatct agttccacac cttttggcac ataaataaat gaaccaccgc
      781 tccatacagc cccatttaag gctgtatatt tattatcggt atatttaaca acggtattaa
      841 aatatttttt aaatatctca ggatgttctt taagtgcctc atcagttgat gaaaaaatca
      901 cacccttttc tttaaattca ctaatcatac tagcgtaaac aacttcggaa tcatattgcg
      961 tcgccacacc agcgagaaat ttacgctcag cttcaggaat acctaacttt tcaaaagttt
     1021 ctttaatcgt gtccggaaca tcgtcccacg agcgtgcctc atcttttaca acacggttaa
     1081 aataagtata agaatcaaaa tcgagatctt ttaaagatgg tccaaattta gggtttttca
     1141 ttaattcaaa cgcgcgaagt gatttaagtc gaaactcaag catccattct ggttcttctt
     1201 taaaggcaga tatttgccga acaacatctt cgttaatacc aataccagta ctagcaattg
     1261 attcttggtc atcggagaaa ccataagcat aagtaccaaa ttttaattca tcagcactag
     1321 tcattgtgat tttcctcctc tttatgtttt gtaattaatt cagcaatccc gtcccaccca
     1381 attgtggcac agcgaatgcg acttggttgc cggactgtat ttgcaaatac aattgcttca
     1441 ttaagaatat ctatgtcata aggttcgctg ttgagcattt ttttgtattc cgcaattatt
     1501 ttcaagacag catcaacatt cattccttca atcatttcaa ggaggattga ggtagaggca
     1561 atcgaaatag tgcaaccaaa tccatcaaaa tatccggtga taactttatt gtctttaact
     1621 tttaaataaa gtgttaaatt atctatacaa c
//
LOCUS       DAOSEX010000210         1635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_77005_length_1635_cov_3.287342, whole genome shotgun sequence.
ACCESSION   DAOSEX010000210 DAOSEX010000000
VERSION     DAOSEX010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77005_length_1635_cov_3.287342"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PK612_03975"
     CDS             complement(<1..593)
                     /locus_tag="PK612_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOH95297.1"
                     /translation="MKLFGTDGIRGPANVLLTPGLSFDIGAFLGQYQKKNKILIAYDT
                     RRSGIVIFASLVAGILSSGGEASSIGVAPTPALAYLCKKYNYDYGIMISASHNPFQDN
                     GIKIFDTNGFKIAESIEEEIEKYIASDTRLKLKTGGEIGKFTDKSELIYEYVDYAKKA
                     FRGGLKLNLLIDGANGSASSVIHHIVAGTSVNATIINV"
     gene            complement(614..1387)
                     /locus_tag="PK612_03980"
     CDS             complement(614..1387)
                     /locus_tag="PK612_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA
                     nucleotide-binding domain protein"
                     /protein_id="HOH95298.1"
                     /translation="MFLILSEMLSELQIQVIQLLVVFVFIVTLDVLLLITLKRIFPKV
                     TLIVLSVVLLVLGLFDLTYAFYLVALIYMLCAMTFIFGNIVEFRWLISNAATPKDRRF
                     QRDFRKIYDQEDLVNQLVSAVFSLSKTKTGALITIERTNQLTELIKNGSPIHAQVSAP
                     LLTTIFYNGTALHDGAVVIRDDTILAASVYFTPSTKALAGKVGSRHRAALGISEISDA
                     VTIVVSEETGRISIAYRAALQPVSPEDFRKVLVDHLLTK"
BASE COUNT          561 a          314 c          254 g          506 t
ORIGIN      
        1 acattaataa ttgtggcatt tacactagtg cctgcaacga tatgatgaat aacactgcta
       61 gcgctcccat tagcgccatc aattaataaa ttaagcttga gtccaccgcg aaatgccttc
      121 ttagcgtaat caacatattc ataaattaac tcggatttgt cggtaaattt gccaatttca
      181 ccgcctgttt taagtttaag tcgtgtgtca cttgcaatat acttttcaat ttcttcttcg
      241 attgattcag caattttaaa accatttgtg tcaaatattt taatgccgtt atcttggaaa
      301 ggattatgcg acgcactaat catgatgccg taatcataat tgtatttttt acataaatac
      361 gctaaggcag gtgttggcgc aacaccaatg gaactagcct cacccccact gcttaaaata
      421 cccgcaacta agctagcaaa aataacaatt ccacttctgc gcgtgtcata agcaattaaa
      481 atcttatttt tcttttggta ttgacctaaa aaagctccaa tatcaaaact taagcctggc
      541 gtcagtaaaa catttgccgg tcctctaatt ccgtctgttc caaataattt catatataac
      601 ctctttaaat aatttatttc gttaataaat gatcgactaa tacttttcta aagtcttcag
      661 gactcacagg ttgtaaagca gcgcgataag caatggaaat gcgaccagtt tcttcactaa
      721 caacaatcgt aacagcatca ctaatttcgc taattcctaa tgctgcgcgg tgccgtgagc
      781 caacctttcc tgcaagtgct ttggttgatg gtgtaaagta gacactagcc gctaaaatgg
      841 tatcgtcacg aattacaacg gcaccgtcat gaagtgccgt cccattatag aaaatagtcg
      901 ttaataaagg tgcactaacc tgtgcatgaa tcggactgcc atttttaatt aattccgtta
      961 attgattagt gcgttcaata gtaataagcg caccagtttt agtttttgat aacgaaaata
     1021 ccgcagatac aagttgattt actaaatcct cttggtcgta aatcttacga aaatcacgct
     1081 ggaagcgacg gtcttttggt gtggcggcat tggagattaa ccatctaaat tcaacaatat
     1141 taccaaaaat aaacgtcata gcgcataaca tataaattaa agcaactaag taaaaggcat
     1201 atgttaaatc aaataaaccg agcactaaca agacaactga aagtacgata agcgtaactt
     1261 tggggaatat acgtttaagc gtaattaata aaagtacatc aagtgtaact ataaaaacaa
     1321 aaacaactaa gagttgtata acctgaattt gcaattcact aagcatttct gaaagtatta
     1381 aaaacataat aaatcccccg tctatttgta taaatatttt agcatgtaaa cacaaccatt
     1441 ttcaatttaa aatgcgtaaa ttgtcgctat tttttaacga aaactaatcc caaattaaaa
     1501 agagtgccat tttgcaaggt tatcgcaata aacattataa ttatttcggc aatataatac
     1561 taaacattat gttcgaatgt tatttaaaat atcagagcac taagcgcgtc ttttaagtaa
     1621 ttcactttta atttt
//
LOCUS       DAOSEX010000211         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_77906_length_1623_cov_2.014668, whole genome shotgun sequence.
ACCESSION   DAOSEX010000211 DAOSEX010000000
VERSION     DAOSEX010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77906_length_1623_cov_2.014668"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(477..1022)
                     /locus_tag="PK612_03985"
     CDS             complement(477..1022)
                     /locus_tag="PK612_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95299.1"
                     /translation="MWQCNKKFENGKKGNKCSTPHLFEREIKEAFIKAFNLLCQKKNS
                     VLNNCKDFITILNNTKELDEKIMVQETEVKVLINIARNLVEENATTALDQEDYKNRYA
                     KIEKKFKEEQDKLDELLKERERKRVQKNSIKLFMKAYKEMPEILKEWSLEVWITIIDH
                     AIVYSDGKMKFIFKSGDEIKV"
     gene            complement(1090..>1623)
                     /locus_tag="PK612_03990"
     CDS             complement(1090..>1623)
                     /locus_tag="PK612_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOH95300.1"
                     /translation="KDHLEAINHKEAIEYVEELVKKYTKLTEYDIKSIHYLILKEIDN
                     LNAGKYRAQNVLISGSKHIPPSYINVPLEMQMLMEQYEDWAVYHPLIRACLLHGEFVK
                     IHPFVDGNGRTARLLLNFELIKNGYPPVIIKKEDRADYYDALEMASTTKNYTLFINLV
                     LNLVNEAADSYLLLLGK"
BASE COUNT          502 a          291 c          212 g          618 t
ORIGIN      
        1 cagatttatg aattttttct attataatat cttcccttgc ctcaacactt ctaatcgttc
       61 agatagtgga attctagata attaaaatta ctcccttcaa taacctttaa aatgtcaaat
      121 tactatcaat tatcgaaaaa atgtattatt tttctatctt tttctaataa aaaatgcctt
      181 gaaattgtat caaagcatac ctagtaagat ggcatgttgt cctaattgat acaatttcgg
      241 aagtaatcgt tcaatcatgc accccccacc cttcaaatcg ttcaaatgtg caacccccta
      301 ggttataatc gttcagacaa taggattccg aaaaaaggaa aagagattcg ctctccagtg
      361 taaataatgg tttggtacta tttaatattt aaaaatgcta actataaatt acattcttga
      421 caagttctta aagtccctgt tttgttaacc atatgaaaca tatttaattt ataaacttat
      481 actttaatct catcaccact tttaaagata aacttcatct taccatcact atatacaata
      541 gcgtggtcaa ttattgttat ccatacttca agagaccact ctttcaatat ttcgggcatt
      601 tctttataag ctttcatgaa taacttaata ctattctttt gcactctttt acgctctctt
      661 tcctttagta attcatcaag tttatcttgt tcttctttaa acttcttctc gattttagcg
      721 tatcgatttt tataatcttc ttggtctaat gcggtagtgg cgttttcttc cactaagtta
      781 cgagcgatat taataaggac tttaacttct gtctcttgaa ccattatttt ttcatctagt
      841 tcttttgtat tattaagtat tgtgatgaag tctttacaat tatttaaaac gctattcttc
      901 ttttgacaaa gtaagttaaa ggctttaata aatgcttctt taatctctct ttcaaataaa
      961 tgaggagtac tacatttatt ccctttttta ccattttcaa atttcttatt acactgccac
     1021 acttctcttt tatatttagt tgttgaatgc gaaacttttg aactcttgag ttcggtgatg
     1081 tacttattat tacttaccga gcaataacaa ataactatca gcggcttcat taactaaatt
     1141 aagcacaaga ttaataaaaa gagtgtaatt tttcgtggta ctagccattt caagagcgtc
     1201 ataataatca gcgcgatctt cctttttaat aatcacaggt ggatagccat ttttaattag
     1261 ttcaaaattt aaaagtaagc gcgcagttcg cccattacca tcaacaaatg gatgaatttt
     1321 cacaaattca ccgtgcagta aacaggcgcg aataagcgga tgataaactg cccaatcttc
     1381 atattgttcc attaacattt gcatttcaag cggcacatta atataggagg gcggaatatg
     1441 ttttgaacca cttattaaaa cgttttgggc gcgatatttg ccagcattta aattatcaat
     1501 ctcttttaaa attaaataat gaatagattt tatgtcgtat tctgtgagtt tagtgtattt
     1561 cttcactagt tcttcaacat attcaattgc ttctttatga ttgattgctt caagatggtc
     1621 ttt
//
LOCUS       DAOSEX010000212         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_78189_length_1619_cov_2.178389, whole genome shotgun sequence.
ACCESSION   DAOSEX010000212 DAOSEX010000000
VERSION     DAOSEX010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78189_length_1619_cov_2.178389"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1619)
                     /gene="feoB"
                     /locus_tag="PK612_03995"
     CDS             complement(<1..>1619)
                     /gene="feoB"
                     /locus_tag="PK612_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOH95301.1"
                     /translation="NIIDSTAIERSLYLTTQLFNFNIPMVIALNMTDLAFEQGIRIDV
                     NKLSKALSVPVVPISCLKRTGIKDLIMLITKDTPTSTNYLTIYSNPLQETINKTAELL
                     PIDAKEFLAIKLLENDIDLAPRIDLDVSKDIKKLNDHYKLGISEVLANERYNFIEKVR
                     DIAVSSREKPKLLTDKIDRILLNKYLAIPIFIIIMLLVYFLAAGPVGMFLSSLVEQGF
                     AALNEGLATFLTNVGASAWSVSLVVDGILTGVTSILSFVPQLLILFFMIDILEASGYM
                     SRIAFFFDRVFRKVGLSGKALIPFIIGTGCSVPAIMASRVIEDERERKLTVLLTPFIP
                     CGAKLPIITLFAAYFFPDSMGLVSASLYFLSIIIILLTALLLKRILYKQKYSPFIQEL
                     PTYKAPNMRYVLRDTGHKTWSFITNAGSVILFASVILWLLLNFNWKMQFGVAIEKSIL
                     SDIGRLFSFVFYPFLGTNSWAAAVSIIQGLITKEQVVSSFSIIAKLSGGNTIFESPLF
                     NFFTPASAYAFMAFNLFSAPCFGAIGAMNHE"
BASE COUNT          566 a          291 c          290 g          472 t
ORIGIN      
        1 aattcatgat tcatagcccc aatcgctccg aaacacggcg cactaaataa attaaaggcc
       61 ataaaagcat aagctgaagc aggagtaaag aaattaaaaa gcggactttc aaagatggtg
      121 ttgcccccac taagtttagc aataattgaa aatgaagaaa caacttgttc tttggtaatt
      181 agcccttgaa taatactaac cgcagccgcc caactatttg tgcctaagaa cgggtaaaac
      241 acaaacgaga ataagcgtcc aatatcactt aaaatactct tctcaatcgc aacgccaaat
      301 tgcatcttcc agttaaagtt aagtaaaagc cataagataa cagaggcgaa taaaataact
      361 gagccggcat tcgtgataaa tgaccatgtt ttatgtcctg tatcacgtaa tacatagcgc
      421 atatttggtg ctttataagt tggtagttct tgaataaatg gtgaatactt ttgcttataa
      481 aggatgcgtt ttaaaagcaa agctgttagt aaaataatga taattgatag gaaatataat
      541 gaggcactta ctagtcccat tgaatcaggg aagaagtagg ccgcaaagag cgtaataatc
      601 ggtaattttg cgccgcatgg aataaaaggc gtaagtaaaa cagttaattt tcgttcgcgt
      661 tcatcttcaa taacgcggct tgccataatc gccggtacac tgcatcccgt accaataata
      721 aaagggataa gtgctttacc cgataaacca actttacgga aaacacgatc aaaaaagaaa
      781 gcaatgcggg acatataacc acttgcttct aagatatcaa tcataaagaa taaaatgagt
      841 aattgcggca caaaactaag aatcgaagtc acgcctgtta agatgccatc aacgactaaa
      901 ctaacagacc acgcagaagc cccaacatta gtgagaaaag ttgctaaacc ttcgtttagc
      961 gcggcaaaac cctgctcaac tagtgatgag agaaacatgc caactggtcc tgctgctaag
     1021 aaatagacaa gtaacatgat aataataaat atcggaattg ctagatattt atttagtaaa
     1081 atccgatcaa ttttatccgt taaaagtttt ggtttttcgc ggcttgaaac agcaatatca
     1141 cgaacttttt caataaaatt atagcgctca ttcgctaata cttcgcttat cccaagtttg
     1201 taatgatcat ttaatttttt aatgtctttg cttacatcaa gatcgatgcg tggcgctaaa
     1261 tcaatatcat tttctaatag ttttattgct aaaaattctt tagcgtcaat cggtaaaagt
     1321 tcggctgttt tattgatagt ttcttgcagt ggattgctat atatagttag gtaattagta
     1381 gaagtagggg tatctttagt aattaacatt attaaatctt ttatccctgt tctttttaaa
     1441 catgatattg ggactacggg aacacttagt gccttgctaa gtttatttac atcaatccga
     1501 atcccttgtt caaacgctaa gtcagtcatg tttagtgcaa tgaccattgg aatattaaaa
     1561 ttaaataatt gcgtagtaag atataagcta cgttcaatcg cggtactatc aataatgtt
//
LOCUS       DAOSEX010000213         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_79112_length_1607_cov_2.774485, whole genome shotgun sequence.
ACCESSION   DAOSEX010000213 DAOSEX010000000
VERSION     DAOSEX010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79112_length_1607_cov_2.774485"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PK612_04000"
     CDS             <1..565
                     /locus_tag="PK612_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95302.1"
                     /translation="KLTDGATIGNTIHVNAWVAALEDAVLNKVTFEGEVKGLGVGGVN
                     AVSKDRATGVVEGQDWTIGGAVFTEGKKVLAARIDTFQVRYENSEGKAVIKSGLKQVD
                     YDKSRVKGKQELKEEYGMKVASPIEKEWYEQAASLVEYIKGKTVSEALGTAAKLANGA
                     EVGCTMNIGGYRTALLEAEHTAFNSRK"
     gene            645..>1607
                     /locus_tag="PK612_04005"
     CDS             645..>1607
                     /locus_tag="PK612_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95303.1"
                     /translation="MGKKKKKTFKKFSATLGVIITLLAIVMYIPRTARIGYKNDKYAN
                     ILKDANIAADTRVVDIAMLGAHDAFSSNINLLSAPDPAESGIVKNKAVNAVFKGGLVR
                     VLKAQQDGAKVLLNRGVRYFDVRISYVEGEWYTKHGLLDTNLFYYLKDIYDFLENRPQ
                     EFVILDVQHIYTGESNINEFLNYLIDLRFVGNPLGLNDFIHHTSAIPLNDLLYFDVLG
                     PTHGGIVLLLKDDGSADVAQKTLFYERNDAIRSKWHNKRYVRDIVPLINEEAAYLKTL
                     ADSDFFRVNQAQLTPDYLKDPLGTLGRWSLINIAATSNAKLLNNK"
BASE COUNT          530 a          253 c          344 g          480 t
ORIGIN      
        1 gaaattaact gatggtgcaa caattggtaa tacaatccat gttaatgctt gggttgccgc
       61 tcttgaagac gctgtcctta ataaagtcac ttttgaaggt gaagttaaag gtttaggcgt
      121 tggtggtgtt aacgctgtta gcaaagaccg cgcaactggt gttgttgaag gacaagactg
      181 gaccattggt ggtgctgtct ttactgaagg caaaaaagtt ttagccgcac gcattgatac
      241 tttccaagtt cgctatgaaa atagtgaagg taaagcagtt attaaatctg gcttaaaaca
      301 agttgattat gataaaagtc gcgttaaagg taaacaagaa cttaaagaag aatatggcat
      361 gaaagttgca agcccaatcg aaaaagaatg gtacgaacaa gctgcaagct tagtcgaata
      421 cattaaaggt aaaaccgtta gcgaagcttt aggaactgct gctaaattag caaatggtgc
      481 tgaagttggt tgtactatga atattggtgg ctatcgcact gctttacttg aagctgaaca
      541 cacagccttt aatagtcgta aataattaat tagttacgaa gaaaagatac acaagggact
      601 acttcaaagt agttcctttt ttgttaatat gcgataatat tactatggga aagaaaaaga
      661 aaaaaacatt taaaaaattt agtgcgacgc ttggagttat cattacatta ctcgcaatag
      721 taatgtatat accgcgtact gcgcgtatcg gttataagaa tgacaaatat gcaaatattt
      781 taaaagacgc taatattgcc gctgatacgc gtgtagtgga tattgccatg ctcggagcgc
      841 atgatgcttt tagcagcaat attaatctac ttagtgcccc tgatccagcc gaatcaggaa
      901 tagtgaaaaa taaggccgta aacgccgttt ttaaaggcgg gttagtgcga gtgttaaaag
      961 cacagcaaga cggcgctaag gtgctactta atcgtggtgt ccgctatttt gatgtgcgga
     1021 ttagttatgt tgaaggtgaa tggtatacaa agcacggttt acttgatacg aatctctttt
     1081 attatttaaa agatatttat gactttttag aaaatcgtcc acaagaattt gtgattttag
     1141 atgttcaaca tatttataca ggcgaaagta atattaatga attccttaat tatttaattg
     1201 accttcgttt tgttggtaat ccgttagggc taaacgattt tatccatcac acaagcgcaa
     1261 taccgcttaa tgatttactt tactttgatg ttttaggacc gactcacggc ggtatagtat
     1321 tattactgaa agatgacggg agcgctgacg tggcgcagaa aacactattt tacgaacgaa
     1381 atgatgcgat tcgttcaaag tggcataaca agcgttatgt aagagatatt gtgccactta
     1441 ttaatgaaga agcagcttat cttaaaactt tagcggatag tgatttcttt agagtcaatc
     1501 aagcacaact cacacctgat tatttaaaag atccactagg gacattaggt aggtggtcac
     1561 ttattaatat tgccgcgact agtaacgcta aattattaaa taataaa
//
LOCUS       DAOSEX010000214         1603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_79386_length_1603_cov_2.756460, whole genome shotgun sequence.
ACCESSION   DAOSEX010000214 DAOSEX010000000
VERSION     DAOSEX010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79386_length_1603_cov_2.756460"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PK612_04010"
     CDS             <1..1262
                     /locus_tag="PK612_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOH95304.1"
                     /translation="QSIVFYDEELGRHDLTLIEGIHRRIGLVEADIKGIEDQKSSLVE
                     ERGKISTNMQILKNERMPETKAIIKERQSKLEENYDAFFINDVAEPFFQNLIAEGKNP
                     LDINAEYRQAFSNALYLSNSVFNQVKKLRKEYVVDYRLTYDVETLHNDDFQMELNDLL
                     EVKLPEYEEKIADAYRKAVKQFKDDFISKLRNAIETVEDQIAELNEALSMSTFGEDSY
                     QFTVKPSTVYRQYYDMIKDDLLLKVTDDDSEFFQKYDDVMKDLFAQIVLAGDGDKNAA
                     VLANVEKFTDYRNYLDFDLIVRNKDGHEQRLSRVLMKKSGGETQTPFYISVLASFAYL
                     YRVNEPGELSNSARLIIFDEAFSKMDSGRIKESIRLLRRFGLQAIISAPSDKVADISP
                     LVDETLVVLRGKNHSTVQLFAREEDLT"
     gene            1282..>1603
                     /locus_tag="PK612_04015"
     CDS             1282..>1603
                     /locus_tag="PK612_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95305.1"
                     /translation="MEAKFVDGIRAYHMRLRRRYLVELFVTISVLLAFCIALIVLDLL
                     SDFNMREPLFVPLLASATSIFLLLFILIYFPIRFKRLQKALNLEFFQRAKTVKLELIP
                     NPLGG"
BASE COUNT          527 a          282 c          321 g          473 t
ORIGIN      
        1 aacaaagcat tgtcttttat gatgaagaac ttggccgtca tgacttaaca ctaattgaag
       61 ggattcatcg ccggattggc ttagtcgaag cagatattaa aggtattgaa gaccaaaaat
      121 caagtttagt ggaagagcgc ggtaagattt ccacaaacat gcaaatttta aagaatgaaa
      181 gaatgccgga aacaaaagca atcattaaag aacggcaaag taaacttgaa gaaaattatg
      241 atgctttctt tattaacgat gtcgccgaac cattcttcca aaacttaatt gccgaaggta
      301 agaatccgct tgatattaat gctgaatatc ggcaagcatt tagcaatgcg ctctacctct
      361 caaatagtgt cttcaatcaa gtgaagaaac tgcgtaaaga gtatgttgtt gattatcgtt
      421 taacatatga cgttgaaact ttacacaatg acgacttcca aatggaatta aatgacttgt
      481 tagaagttaa attaccagag tacgaagaaa aaattgcgga tgcatatcgt aaagcggtta
      541 aacaatttaa agatgacttc atttcgaaat tgcggaatgc aattgaaacg gttgaagatc
      601 aaattgctga acttaacgaa gcacttagta tgtctacatt tggtgaagac tcgtatcaat
      661 tcacggttaa accaagtaca gtatatcggc aatattacga tatgattaaa gacgatttat
      721 tacttaaagt aacggatgac gatagtgagt tcttccaaaa atatgacgat gtgatgaaag
      781 atttattcgc gcaaatcgtg ttagcgggag acggtgataa aaatgcggca gtgctcgcta
      841 atgttgaaaa attcaccgat taccgtaatt atttagactt tgacttaatt gtccgcaata
      901 aagatggcca cgaacaacgc ttatcacgcg tattaatgaa gaagtctggt ggggaaacac
      961 aaaccccgtt ctacattagt gtacttgcta gttttgctta tctctatcgc gttaatgaac
     1021 caggtgaact tagcaacagc gcccgtctta ttatctttga cgaagcgttt agtaaaatgg
     1081 atagtgggcg gattaaagaa tcaattcgct tattacgccg ctttggctta caagcaatta
     1141 ttagtgcccc aagtgataaa gtggctgata ttagcccctt agtcgatgaa acacttgttg
     1201 tattacgcgg taaaaatcat agcacagtgc aattatttgc gagagaagag gacttaactt
     1261 aaccgcaaag aaaggagaat tatggaagca aagtttgttg acggaataag agcatatcac
     1321 atgcgcttac gtcgccgcta cttagtggaa ctatttgtta ccattagcgt tttactcgcc
     1381 ttttgcatcg ccttaattgt ccttgatctc ttgagtgact ttaatatgcg tgaaccactt
     1441 tttgttccgc tattagcgag tgctactagt attttcttac tcttatttat tcttatttat
     1501 ttcccaattc gctttaaaag gctgcaaaaa gcgctcaatt tagagttttt ccaaagagca
     1561 aagacagtaa aacttgaact tattcccaat ccgttaggcg gca
//
LOCUS       DAOSEX010000215         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_79989_length_1595_cov_2.680519, whole genome shotgun sequence.
ACCESSION   DAOSEX010000215 DAOSEX010000000
VERSION     DAOSEX010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79989_length_1595_cov_2.680519"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..826
                     /locus_tag="PK612_04020"
     CDS             65..826
                     /locus_tag="PK612_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95306.1"
                     /translation="MTKVKTKKGPKIILAVASLLVVTILSLSIVRIVKNNPPKHKDYA
                     IYLYGEEHGVAKINKKELALWDDFYHKQGMRHLFIESAYFDAAILNLWMQEEDDTRFE
                     FLYDGWEGTLSHNKETRDFYFAFKESYPETIFHGTDVGHQHERAGQYYLDYLIDNGLE
                     NTEAYTLTLESIAQGEEFTKTGDLKYRESKMVSNFIREFDALDNEVIMGIYGSAHISK
                     DFIAFFLGIEPMTIQLKKHYEPQNILIYAKELTRL"
     gene            906..982
                     /locus_tag="PK612_04025"
     tRNA            906..982
                     /locus_tag="PK612_04025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:940..942,aa:Arg,seq:tct)
     gene            1170..>1595
                     /locus_tag="PK612_04030"
     CDS             1170..>1595
                     /locus_tag="PK612_04030"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOH95307.1"
                     /translation="MKDLATLVKEFKTIIDNSNNVVFFGGAGVSVPSGIPDFRSADGV
                     YSKDTNTMYSAEEMVSRTMYYRNTPFFNDFYFKYLVYPDALPNPCHTYLARLEKEGKL
                     KAVVTQNIDGLHQAAGSKVVYELHGSVHRNYCTLCKAFHD"
BASE COUNT          541 a          258 c          299 g          497 t
ORIGIN      
        1 gaagtagcgg aagccttact tactaaatta aatagggttg atgacacgga aggaggagtt
       61 taatatgact aaagtaaaga ctaaaaaagg accaaaaata attcttgccg ttgcctcact
      121 cttagttgtt actattcttt ctttaagcat tgtacgcatt gttaaaaata atccgcccaa
      181 gcataaagat tatgccattt atctctatgg cgaagaacat ggggtcgcta aaatcaataa
      241 aaaagaacta gcgctctggg atgactttta tcataagcaa ggtatgcgtc atcttttcat
      301 cgaatcagcc tattttgatg cagcaattct taatttatgg atgcaagaag aagatgacac
      361 acgctttgag tttctttatg atggttggga aggaacatta tcgcataata aagagacccg
      421 tgacttttat tttgccttta aagaaagtta tccagaaaca atattccacg gcactgatgt
      481 tggccatcaa catgaaagag caggacagta ttacttagat tatcttattg ataacggttt
      541 agaaaacacc gaagcataca ctttaacact tgaatcaatc gctcaaggcg aagaatttac
      601 taaaactggc gacttaaaat atcgtgaaag taagatggtc agtaacttta ttcgtgaatt
      661 tgatgcttta gataatgaag tgataatggg gatatatggt agtgcacata ttagtaaaga
      721 ctttattgct ttctttttag gcattgaacc aatgacaatt caacttaaaa agcattacga
      781 accgcaaaat atattaatat acgctaaaga actcacacga ctttaaaagc taaatttaac
      841 aataaaacta ttttttaatg tcacttgttg aaactaatat tcttttattg taaaataact
      901 tgcgtgtccc ggtagctcag tcggatagag caacagcctt ctaagctgtg ggtcgggcgt
      961 tcgagtcgct cccgggacgc cattaattaa tgaagaagag ttagtgttta ccccctagat
     1021 gctgactctt tttttctaac attaaattga actagttttg tgataaaaac ataacataaa
     1081 ggctaatggt aattaattag taaataatta gtagaatatt tagtagagaa agcattaaat
     1141 agcttattct taagtgttat aataaaagta tgaaagattt agcaacatta gtgaaagagt
     1201 ttaagactat tattgataat agtaataatg ttgtcttttt tggtggcgct ggtgtgagtg
     1261 tccctagcgg tattcctgat tttagaagtg ccgacggagt gtatagtaaa gacacaaata
     1321 caatgtactc agcagaagaa atggtatcac gcacgatgta ttaccgtaat actcctttct
     1381 ttaacgactt ttattttaag tatcttgtct atcccgatgc gcttcctaat ccgtgccaca
     1441 cttatttagc tagacttgaa aaagaaggta aattaaaagc agtagtgaca caaaatattg
     1501 acggtttaca tcaagccgct ggttcaaaag tcgtctatga acttcacggc agtgtgcacc
     1561 gtaattactg tactttatgc aaagcatttc atgat
//
LOCUS       DAOSEX010000216         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_80677_length_1586_cov_3.350098, whole genome shotgun sequence.
ACCESSION   DAOSEX010000216 DAOSEX010000000
VERSION     DAOSEX010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80677_length_1586_cov_3.350098"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PK612_04035"
     CDS             complement(<1..906)
                     /locus_tag="PK612_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011068386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH95308.1"
                     /translation="MYLKRKIDEYLVNWKKDNNKMPLIVKGARQVGKTESIKKFAENN
                     YENVIYINFVEEPIYKGITADGYKTDAIIKIMSRIDPLKVFTPYKTLIFFDELQDFPD
                     ITTALKFFKLDGRFDVICSGSLLGINYRIIESNSVGYKVDYEMNSLDFEEFLWAKGYS
                     DDVVRDMLKHMCELKPFNDVDMKIFNDLFLDYAILGGMPAVVRLYIENGTFEKTLELQ
                     KQLILDYKEDVRKYVVGLDQTRILNVFNHIAPQLAKENKKFQITKVGKNARFADYRGC
                     IEWLKDSGVINVCYGLNYPELPLKGN"
     gene            complement(1233..1308)
                     /locus_tag="PK612_04040"
     tRNA            complement(1233..1308)
                     /locus_tag="PK612_04040"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1273..1275),aa:Ala,seq:cgc)
     gene            1447..>1586
                     /locus_tag="PK612_04045"
     CDS             1447..>1586
                     /locus_tag="PK612_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HOH95309.1"
                     /translation="MEKVYAVIDLKSFYAGVECAERGLDPFTTPLVVCDERRGDGTII
                     MAV"
BASE COUNT          529 a          291 c          226 g          540 t
ORIGIN      
        1 attgccttta agcggaagtt ctggataatt taacccatag catacattaa taacccccga
       61 atcttttaac cattcaatac agccgcggta atcagcaaaa cgggcatttt tgccaacttt
      121 tgtaatttga aattttttat tttctttcgc taattgtggt gcaatatgat taaaaacatt
      181 caaaatgcgt gtttgatcta aaccaacaac atatttgcgg acatcttctt tataatccaa
      241 aataagttgt ttttgtaatt caagtgtttt ttcaaatgta ccattttcaa tataaagtct
      301 gacaaccgca ggcatccccc caagaatagc gtaatctaaa aataaatcat taaagatttt
      361 catatcaacg tcattaaatg gcttcaactc acacatatgt tttagcatgt cacgcacaac
      421 atcatcacta tagcctttgg cccacaaaaa ttcttcaaaa tcgagtgaat tcatttcata
      481 atctacttta tagccaacgc tattactctc aattatccta taattaatac caagcagtga
      541 accgctacag atgacatcaa agcgaccatc taatttaaaa aacttcaacg cagtggtgat
      601 atcaggaaaa tcttgtaatt catcaaaaaa gattagtgtt ttatatgggg taaatacttt
      661 aagcggatca atccgtgaca taattttaat aatcgcatct gttttatacc catccgcagt
      721 aataccttta tatattggtt cttccacgaa attaatataa atgacatttt cataattatt
      781 ttcggcaaat tttttaattg actcagtctt accaacttgt cgtgcacctt taacaattaa
      841 aggcattttg ttattatcct ttttccaatt aactaaatac tcatcaattt tacgtttaag
      901 ataaatcata atcatccctc atcatcatta aatttgtaat tcaatattaa cactataatc
      961 tatatataac aagccttttt acaaaaaaag gagcgagatt tttccaaaat ttacaaaaaa
     1021 aggagcgact ttttggcaga atttacaaaa aaatgaggga gttttatatt taaaaacatg
     1081 taaacattca tataatcaat atttttcgtc gcttatctaa ttaatgacac aattttataa
     1141 attttgcttc ttttttggtt ttcaaaataa atggtttttg ttattaataa ataaaaaaat
     1201 agctccctga gcggaagcta atacttttac tttggtggag ccgacgagga tcgaactcgc
     1261 tacctcatcg ttgcgaacga tgcgctctcc cagatgagct acggccccaa gtctaactaa
     1321 aagtttatca aactagtaaa taattgcaag ggctatcgct tatcaatttt aatggtgaaa
     1381 atcttttact aactttgttc atccttctgt ctaaaaatta aagtgtcctc ttgtataatt
     1441 atggtaatgg aaaaagtata tgccgttatc gatttaaaaa gtttctacgc gggcgtagaa
     1501 tgtgcagagc gtggtcttga tccctttacc actcctttag tggtgtgcga tgagcggcgc
     1561 ggagacggca ctattattat ggctgt
//
LOCUS       DAOSEX010000217         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_80933_length_1583_cov_3.010471, whole genome shotgun sequence.
ACCESSION   DAOSEX010000217 DAOSEX010000000
VERSION     DAOSEX010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80933_length_1583_cov_3.010471"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PK612_04050"
     CDS             complement(<1..241)
                     /locus_tag="PK612_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOH95310.1"
                     /translation="MKKQIVIATNNANKLKEFKSLFANTPYEVLSLADINFTGVIKED
                     GESFSDNAVIKAMTIHKLTNLPVIADDSGLSVNALE"
     gene            complement(263..1309)
                     /locus_tag="PK612_04055"
     CDS             complement(263..1309)
                     /locus_tag="PK612_04055"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOH95311.1"
                     /translation="MSNLVGYSIVALFVIVMGLLLLLKVYLQTYHPGKYWYIERPIKY
                     LMILGPMFFLFAIGERWHFGENFLPSQNPDDLAWGPFHLGWLFAMVIAIIVVSSGVKA
                     DKANTKRYVFGQLNKIDFTVFQFGVLLFGIELYKQLIFLNLYEGLANYHWYGFPLQFC
                     SIPIFLYPLTPFIKNEKIKEAIYSFISIFNLIGGLAVMILATGVYTLQVSISIHTMIW
                     HGVMVVVAFYLINAYKIGTKWRHYLGAVTVLFCLIVLAQLTNVLFHYIGMKFPGPGDF
                     DGFFISPWIDRRNMPILGDIRANMIAGGVPTLIIALVFPHIYFVIFSLTGLLIYYLFH
                     FIWKDVEKNKRKKH"
BASE COUNT          596 a          266 c          228 g          493 t
ORIGIN      
        1 cttctaaagc gttaacactt agtccactat catcagcgat aacaggtaaa ttcgtcaatt
       61 tatgaatagt catagctttt ataaccgcat tatcactaaa cgattcgccg tcttccttta
      121 taacgccagt aaagttaatg tccgccagac ttaaaacttc gtaaggcgta ttagcaaaaa
      181 gtgacttaaa ttctttaagt ttatttgcgt tattcgtggc aatgacaatt tgttttttca
      241 taattttata aagtattagt ttttaatgct ttttcctttt atttttttca acatctttcc
      301 aaataaaatg gaataaatag taaataagta gcccagttaa actaaagatt acaaagtaaa
      361 tatgcgggaa cactagtgca atgattagtg tggggacacc accagcaatc atatttgctc
      421 taatatcacc aagtatcggc atatttctcc tgtcaatcca tggtgaaata aagaaaccat
      481 caaaatcccc tggaccaggg aacttcatac caatatagtg gaataaaaca ttcgttaatt
      541 gcgctaaaac gatcaaacaa aataatactg tgaccgcgcc aagataatgc cgccatttag
      601 tgccaatttt ataagcattt attaaataga aagcaacaac aaccattact ccgtgccaaa
      661 tcatcgtatg aatcgatata gaaacttgta gagtatatac accagtggca agaatcatca
      721 cagcaagacc accaataaga ttgaagattg aaataaagga gtaaatggct tctttaatct
      781 tctcattttt aataaatggt gtaagtggat ataaaaagat tggtattgaa caaaattgta
      841 aggggaaacc gtaccaatga taattagcaa gcccttcata taaatttaag aaaattagtt
      901 gtttgtataa ttcaatgcca aatagtaaaa cgccaaattg aaaaacagta aaatcaatct
      961 tatttaactg accaaaaaca tagcgtttcg tgttcgcctt gtcggcttta acaccacttg
     1021 aaacaacaat gattgcaata accatggcaa agagccatcc aagatgaaac ggaccccacg
     1081 ctaagtcatc tggattttgc gatggtaaaa agttttcacc aaaatgccat ctttcaccaa
     1141 ttgcaaacaa aaaaaacatt ggccctaaaa tcattaaata ttttattggt cgctcgatat
     1201 accaatactt accaggatga taagtttgta aatatacttt aagcaatagc aacaaaccca
     1261 ttactataac gaataacgct actatactat aacctactaa atttgacata cactctcctt
     1321 gaggcagatt gaagttatta tataacttat tgctttttta ttctattgaa agagattaat
     1381 gttcgtttta tacttaaaaa tattgcgaaa gaaaatgatt aaaatgatta attaatatct
     1441 aaaaatccaa aatcagtaat ttgttgttct attgttttga cttcatttga tgcggttttt
     1501 aatttcttaa aattatgagt taaattataa ataaatggaa caataaggat aagaataact
     1561 aaaagtaaaa agattataat att
//
LOCUS       DAOSEX010000218         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_81401_length_1577_cov_3.397503, whole genome shotgun sequence.
ACCESSION   DAOSEX010000218 DAOSEX010000000
VERSION     DAOSEX010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81401_length_1577_cov_3.397503"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PK612_04060"
     CDS             <1..894
                     /locus_tag="PK612_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOH95312.1"
                     /translation="IFITGEITTKAKINAKAITKRVLSDVGYESDKYTIINNIGLQSP
                     DIAMGVDDGGAGDNGMMFGYACNDTEKYLPTAMVILQELAKGYDELRQSLSGLFYPDG
                     KAQITGIYDDDFNLIKIKDFTICYQNSENDRLKTDAIIKKLADNICEKYGVKVERYLF
                     NPTGKFLIGGFDGDAGLTGRKIVVDAYQSFANVGGGCMNGKDPTKVDFSAAHKARQIA
                     LRYLKEHNLKWCEVQLSYAIGVKQPLAIYIVSDKGNIEAPESLYKECEPQEIIKDLKL
                     REISYEELAQFGHFGGKNEKY"
     gene            881..>1577
                     /locus_tag="PK612_04065"
     CDS             881..>1577
                     /locus_tag="PK612_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008470959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOH95313.1"
                     /translation="MKNINIIYKKIDELKPYKNNPRKNDKAVKAVADSIKEFGFKVPI
                     ILDSNMEIIAGHTRLKAAKRLKYKEVPCIIADDLTPDQVRAFRLVDNKVGELAEWDID
                     LLNIELSEIELDLTPFEFKIEKPLNDIEEDDYEVVLPDEPKSKKGDIYILGNHRLMCG
                     DSTNKDDVRKLMNGNQCDMIFTDPPYNVNYEGGTGMKIQNDNMLDATFYDFLISAFDN
                     MFENTKRGGAIYVC"
BASE COUNT          597 a          216 c          331 g          433 t
ORIGIN      
        1 atatttatca ctggagaaat aactactaaa gcaaaaatca acgccaaagc aatcacaaag
       61 cgtgttttaa gcgatgttgg gtatgaaagc gataaataca ccatcatcaa taatatcggg
      121 ctacaatcgc ctgatattgc gatgggagtg gatgatggag gagcagggga caacggtatg
      181 atgttcggtt atgcctgtaa tgatactgaa aaatatttac caaccgctat ggttatatta
      241 caagaattag ccaaaggcta tgatgaattg cgacaaagct tatcagggtt attttatccg
      301 gatggaaaag cacaaataac gggcatatat gatgatgact ttaatcttat aaagattaaa
      361 gatttcacta tatgttatca aaacagcgaa aatgatagat taaaaaccga tgctatcata
      421 aaaaaattag cagataatat ttgcgaaaaa tatggagtta aagttgaaag gtatcttttc
      481 aatccaactg gtaaatttct cattggtggt tttgatggcg atgccggatt aactgggcga
      541 aagattgtgg tagatgcata tcagtcattt gctaatgttg gtggcggttg tatgaacggg
      601 aaagacccaa ctaaagttga ttttagtgca gcccataaag cgaggcaaat agcgcttaga
      661 tacctaaaag aacataatct aaaatggtgc gaggttcaac tatcgtatgc aatcggggtt
      721 aaacaaccgt tagccattta tatagtgagt gataaaggaa acattgaagc acctgaatcg
      781 ctttataaag aatgtgagcc acaagagatt ataaaagact taaaattaag agaaatatcg
      841 tatgaggaat tggcgcaatt cggtcatttc ggaggaaaaa atgaaaaata ttaatattat
      901 atacaaaaaa atcgatgaat taaaaccata taaaaacaat ccgagaaaaa atgacaaagc
      961 agtaaaagcg gttgccgact caataaaaga attcggtttc aaagtaccaa ttatccttga
     1021 tagcaatatg gaaattatcg ctggacacac aagacttaaa gcggctaaaa gattaaaata
     1081 taaagaagtg ccatgtatta tagctgatga tttaacgcct gatcaagtga gagcgttccg
     1141 acttgtcgat aataaggttg gcgaattagc ggagtgggat atcgatttat taaatattga
     1201 attatcagaa atagaacttg atttaacacc atttgagttt aaaattgaaa aaccgctgaa
     1261 tgacatagaa gaagatgatt atgaagtggt gttgcccgat gaaccaaaaa gtaaaaaggg
     1321 cgatatttat attttgggta atcatagatt aatgtgtggt gattcaacaa ataaagatga
     1381 tgtgagaaaa ttaatgaatg gcaatcaatg cgatatgatt ttcaccgatc ctccgtataa
     1441 tgttaattac gagggcggaa caggcatgaa aatacaaaat gataatatgt tagacgcaac
     1501 cttttatgat ttcttaatta gtgcatttga taatatgttt gaaaacacta aaaggggtgg
     1561 agcaatctat gtctgtc
//
LOCUS       DAOSEX010000219         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_82352_length_1565_cov_2.524503, whole genome shotgun sequence.
ACCESSION   DAOSEX010000219 DAOSEX010000000
VERSION     DAOSEX010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82352_length_1565_cov_2.524503"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PK612_04070"
     CDS             <1..373
                     /locus_tag="PK612_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95314.1"
                     /translation="TFVRNIHGDYYLVPKTYEKGNNRNFLFFENDTFYIVKIVEAVNT
                     AKLAAKDDDLNSYEHFKTEAEIAEIVAHVTEALAKLDATKTNALNFYMKDLKIVFHDE
                     DVYDFFKEKFPDIFDDEKKKK"
     gene            475..891
                     /locus_tag="PK612_04075"
     CDS             475..891
                     /locus_tag="PK612_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOH95315.1"
                     /translation="MSKDCVFCKIIRREIPGAIVYEDNDVLAFLDISPTAPGHTLVIP
                     KEHHETFLGTPRKLMYNVMDVAQRIGQVQIKTLKAQGVNILINNYPPAGQSVPHFHVH
                     VIPRYGKKDGLKIAMVEHLDGDLNFPAVVEKIKSGL"
     gene            complement(892..>1565)
                     /locus_tag="PK612_04080"
     CDS             complement(892..>1565)
                     /locus_tag="PK612_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH95316.1"
                     /translation="RLDEFTMASPVKLETLTAELDRLLSSFTDTDIQRLVERLISDRY
                     EAFITYPAASRLHHEYTHGLLEHTITIANLADRIIDYYLELYEGDWHINRDLVIAGIL
                     LHDLGKTIELSGPVLTSYTVAGRLVGHISLMNAELLKVAEELEIKSEMPILLSHIILS
                     HHGKLEYGSPVTPMTKEAQLVSMIDDLDAKMKLIEKALNTTNSGEFTDRLWALDSTSF
                     YKPKK"
BASE COUNT          502 a          308 c          274 g          481 t
ORIGIN      
        1 aaccttcgtt cgtaatattc atggcgatta ttacctagtc cccaaaactt atgaaaaagg
       61 taacaatcgt aacttcctct tcttcgaaaa cgatacattc tacatcgtta aaatcgttga
      121 agccgttaac accgctaaac ttgccgctaa agatgatgac ttaaattcat atgagcattt
      181 caaaactgaa gcagaaattg cggaaattgt tgcccatgtt actgaagctt tagcgaaact
      241 cgatgcgact aaaactaatg cgcttaactt ctacatgaaa gacttaaaga ttgtcttcca
      301 tgacgaagat gtctatgatt tcttcaaaga aaaattccct gatatcttcg atgatgaaaa
      361 aaagaagaaa taagcatacc ttctatttat taaataacga ccgcaggtta acccctgcgg
      421 tttttattta cagatatttg ttataattaa tatatagata aaggaggaac actaatgagt
      481 aaagattgtg ttttttgtaa gataattcgc cgagaaatcc caggtgccat cgtctatgaa
      541 gataatgatg tccttgcttt tcttgatatt tccccaaccg ctccgggtca cactttagtt
      601 attcctaaag aacaccacga aacattttta ggcactccgc gtaaattaat gtacaacgtc
      661 atggatgtcg cgcaacgtat cggccaagtg caaattaaaa cacttaaagc gcaaggcgtt
      721 aatattttaa ttaataatta tccgcccgct ggtcaaagcg tcccgcactt tcatgtccat
      781 gtaatcccgc gttatggtaa aaaagacgga ttaaaaatcg ccatggttga acaccttgat
      841 ggcgatttaa acttcccagc agtcgtggaa aaaattaagt ctggtttata attatttttt
      901 cggtttataa aaactcgttg aatcaagcgc ccacaaccga tcagtgaatt caccgctgtt
      961 tgttgtgttg agcgcttttt caattaattt cattttggca tctaagtcat caatcatact
     1021 cacgagttgt gcttcttttg tcattggagt tacaggtgaa ccgtattcaa gcttaccgtg
     1081 atgcgataag atgatatgtg ataaaagaat tggcatttca cttttaattt caagttcttc
     1141 tgcaactttt aaaagttcag cgttcattag tgaaatatgg ccaacaagtc taccagcaac
     1201 tgtataacta gttaacactg gtccacttaa ttcaattgtt ttacctaagt catgcagtaa
     1261 aatgcccgca ataactaaat cgcgattaat atgccaatca ccttcataca actctaaata
     1321 ataatcaatg atacgatctg caagattagc gatggtaatt gtgtgttcaa gcaaaccgtg
     1381 tgtatattca tgatgtaatc tgctcgcggc aggataagtt ataaaagctt cataacgatc
     1441 actgattagt cgttccacga gccgctgaat atccgtgtcc gtaaatgaag agagtaaacg
     1501 atcaagttcc gcagttaaag tttcaagttt aaccgggctt gccattgtaa attcatcaag
     1561 tctaa
//
LOCUS       DAOSEX010000220         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_82373_length_1565_cov_1.988742, whole genome shotgun sequence.
ACCESSION   DAOSEX010000220 DAOSEX010000000
VERSION     DAOSEX010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82373_length_1565_cov_1.988742"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PK612_04085"
     CDS             complement(<1..906)
                     /locus_tag="PK612_04085"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOH95317.1"
                     /translation="MKILMVASESSPLAKTGGLADVVYALSKELAILGEEVSVVMPFY
                     GNIKRSPDQEIRFVANVQVPLGWRFQNAKVYKTFIDGITFYLLENEYYFGREGFYGYD
                     DDTERFAFFSVAVRNMMQALKIKPDVVHVHDYHGGMVPALIKIQNSRTKFFNKMKFVV
                     TIHNPAFQGEFHPDLLPDFYDIRKEYFDDGTLRYRDKVNALKAAIVFADKINTVSPTH
                     AEELLTPEGSKGLDGILKLRKNDFSGILNGIDYDEFNPQTDKHLPFRYGKTRPGIKTK
                     NKLVLFKELGIKGDVDTPLFSMVSRL"
     gene            complement(906..>1565)
                     /locus_tag="PK612_04090"
     CDS             complement(906..>1565)
                     /locus_tag="PK612_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOH95318.1"
                     /translation="NNARNEYIGRAIFDLNKDGHVINISRNKGEKDARNIGLEIYIFN
                     RETFDFLIRDAQNISSFYSLSDIVAYHALKDIRVDTYEYVGYMRCIDSYQHYYDYSFE
                     LLNYRIRRQLFLHNWPIYTVTHDTPPAKYEATADVKNAFIANGSLISGMIKNSIISRN
                     VKIEEGAVVKNSIIFTDSILRRNKVISHAIIDKKAEVAVAAELAGTPEDILYVRKGDR
                     V"
BASE COUNT          483 a          322 c          261 g          499 t
ORIGIN      
        1 taaacggcta accatcgaaa atagcggtgt atcaacatca cctttaatac ctaattcttt
       61 aaatagtaca agtttgtttt tcgtcttaat tcctggtcta gttttaccat atctaaacgg
      121 taaatgttta tcagtctgtg gattaaattc atcgtaatcg atgccgttaa gaataccgct
      181 gaagtcattt ttccgtaatt ttaaaatacc gtctaaccct tttgaccctt caggggttaa
      241 aagctcttcc gcgtgcgtag gactaacggt attaatttta tcagcgaaga caatcgcagc
      301 tttaagcgca ttaaccttgt cacgataacg taatgttccg tcatcaaaat actccttccg
      361 aatatcataa aaatcaggga gtaaatccgg atggaattca ccttggaatg ctggattatg
      421 aattgtgaca acaaatttca ttttattaaa gaattttgtg cgtgaatttt gaattttgat
      481 taacgctggt accatgccgc catggtaatc gtggacatgg acgacatctg gtttaatttt
      541 taatgcttgc atcatattgc gaacagcgac actaaaaaac gcaaagcgtt ccgtatcatc
      601 gtcatatcca taaaaccctt cacggccaaa atagtattca ttttctaata aataaaaggt
      661 aataccgtca ataaatgttt tatacacttt agcgttttga aagcgccagc ctaaagggac
      721 ttggacattc gcgacaaagc ggatttcttg atcaggactc cgtttaatat tgccgtaaaa
      781 tggcatcact acacttactt cttccccaag aatcgcaagt tccttactta aggcatagac
      841 gacatcggct aagccaccag tttttgcaag tggactactt tcacttgcta ccattaatat
      901 tttcattaca ctcgatctcc ttttctaaca tatagtatat cttccggcgt acccgctagt
      961 tcagcagcaa ccgccacttc cgccttctta tcaataatag catgactaat cactttattg
     1021 cgtctaagga ttgaatcggt aaagataatg gaatttttca caaccgcccc ttcttcaatc
     1081 ttgacattcc gtgaaataat cgaattttta atcatcccgc tgattaatga tccgttggcg
     1141 ataaacgcat ttttgacatc agccgttgct tcatacttcg ctggtggggt atcatgcgtt
     1201 acggtgtaaa tcggccaatt atgtaagaag agttgacgac ggatacgata gttaagtaat
     1261 tcaaaactat aatcataata atgctgataa ctatcaatac agcgcatata tccgacatac
     1321 tcataagtat cgacccttat atcttttaag gcatggtaag caacaatatc acttaaggaa
     1381 taaaatgagc taatgttttg ggcatcacga atgaggaaat caaatgtttc acgattgaaa
     1441 atataaattt caagtccgat attgcgtgca tctttttcac ctttatttcg tgaaatatta
     1501 ataacatgac cgtccttatt taaatcaaag atagcgcgtc caatatattc atttcgcgca
     1561 ttatt
//
LOCUS       DAOSEX010000221         1551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_83540_length_1551_cov_4.130348, whole genome shotgun sequence.
ACCESSION   DAOSEX010000221 DAOSEX010000000
VERSION     DAOSEX010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83540_length_1551_cov_4.130348"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..730)
                     /locus_tag="PK612_04095"
     CDS             complement(5..730)
                     /locus_tag="PK612_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95319.1"
                     /translation="MKKKKLLFKIIGAMTLALPISITLVVSAIWSKPTPDYVITFNDA
                     LAVDLETINEKVVITEHDDKNKALVIAFDEFTFYNIDFTYYDKIIDGKYIVKNKSYIR
                     FLSDDFDKVLEIIDGKNEQGDPFIKLDKVSHINIQTGNKLSLSLIVSIIATLVVVGII
                     SGKMKLHRKYPKSATFIALLSATIIIGVINAIIGSIFTVFVVATISWGAYCLEDAYFK
                     SLQPVSESQQQQNALIDELRRLL"
     gene            complement(705..1181)
                     /locus_tag="PK612_04100"
     CDS             complement(705..1181)
                     /locus_tag="PK612_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95320.1"
                     /translation="MKKVKKIIKYLSIAISFGFIALSGVEIISGLIGDVSLFGFNLTE
                     VQQKITHYITSGGLAIVGGLGVGLNDFLNRKLIESRNLTNQALQSAISVGEKYGEIKK
                     EVRELCILIKQLAADNSHLAELITADLSAKLSNPLIDKKAKELIEEAINEKKETII"
     gene            complement(1178..1447)
                     /locus_tag="PK612_04105"
     CDS             complement(1178..1447)
                     /locus_tag="PK612_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95321.1"
                     /translation="MAKIVLDVAINNLQKDMILVYNPANEKWEATSKSAYLNQTYIEI
                     DKINQKTVELKEKTIELERKLAETQTKFDELRRIFENTIKELISR"
BASE COUNT          456 a          302 c          227 g          566 t
ORIGIN      
        1 ttggtcataa gagcctccgt aattcatcga ttaaagcgtt ttgttgttgt tgtgattcgc
       61 taacgggttg taaagactta aagtaagcgt cctctaaaca ataagcaccc caactgatag
      121 tcgccactac aaagaccgta aatatactac caatgatggc attaattacc ccaattataa
      181 tggtggcact taataaggca ataaaagttg ccgactttgg atattttcgg tgcaacttca
      241 ttttaccact gataatgccc acaactacta aagtggctat gatactcacg ataagtgata
      301 aggacagttt attacccgtt tgaatgttga tgtggctcac tttatccaac ttgataaatg
      361 gatcaccttg ttcatttttg ccatcgataa tctctaaaac tttgtcgaaa tcatcactta
      421 aaaaccgaat gtatgactta tttttaacaa tgtattttcc atcgataatt ttatcgtaat
      481 aggtaaaatc gatattataa aaagtgaatt catcgaaagc aatgactaac gctttgtttt
      541 tatcatcatg ttcggttata accacttttt cattaatcgt ttctaaatca accgctaatg
      601 catcgttaaa agtgattaca taatcgggag taggcttcga ccaaatggcg cttactacca
      661 atgttattga aatcggtaaa gcaagtgtca tcgcaccgat gattttaaat aatagtttct
      721 tttttttcat ttattgcttc ctcgattagt tcttttgctt ttttatcaat taacgggttg
      781 cttaattttg cagataaatc ggcggtaatt aactcggcta aatgtgaatt atcggcagca
      841 agctgtttaa tcaaaatgca taattctctc acttcttttt taatctcgcc atatttttca
      901 ccaacgctaa tagccgattg tagggcttga tttgtaagat tccgactttc tattaacttg
      961 cggttcaaaa agtcgtttaa acccacgcca agcccaccaa cgatggctaa accaccactc
     1021 gtgatgtagt gagtaatctt ttgttggact tcggtcaggt taaatccgaa taaactgaca
     1081 tcgcctatta gcccactgat aatttcgact ccgcttaaag caataaaacc gaaacttata
     1141 gcaatcgaca aatacttaat tatctttttt acttttttca tcgtgaaatt aactccttaa
     1201 tggtattttc aaatattctc cgcagttcat caaatttcgt ttgcgtttca gctaactttc
     1261 gctctaactc gatggtcttt tcttttaatt cgaccgtctt ttgattaatt ttatcaattt
     1321 cgatataagt ttgatttaaa tatgcgctct tgcttgtcgc ctcccacttt tcattagcgg
     1381 gattatacac taaaatcata tctttttgta aattattaat cgccacatct aatactattt
     1441 ttgccataaa ttactccctt acttatatta tacgatattt ttgtaaattg tcattatttt
     1501 cattagcatt taattctttt atttcgccca caagattgtt atttctatca t
//
LOCUS       DAOSEX010000222         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_84511_length_1540_cov_3.041751, whole genome shotgun sequence.
ACCESSION   DAOSEX010000222 DAOSEX010000000
VERSION     DAOSEX010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84511_length_1540_cov_3.041751"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PK612_04110"
     CDS             complement(<1..549)
                     /locus_tag="PK612_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95322.1"
                     /translation="MKIRRLLLALIGVTLLASCEPPTSQSIEVPESEIPSVSDISDTP
                     SIVSETSTTEEVTSVEESSVEVSSEWVEPEPVLKPAENWNFRPLPAYTGNFWNTIDFT
                     LRGEELRQALADYMWSKFYVPGVLDNRVTYNVAYDAIKVMDADPNKPGNVLSVYDLRS
                     HPHAGYNYGKWNREHVFPQSKLA"
     gene            complement(616..>1540)
                     /locus_tag="PK612_04115"
     CDS             complement(616..>1540)
                     /locus_tag="PK612_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH95323.1"
                     /translation="GIPLKRIMESEKEKILSLYDELKKRVLGQDVALRLVSDAILRQR
                     AGLNNPNRPIGSFLFLGPTGVGKTEVAKSLAYALFDDERHIVRIDMSEYMEKFSVSRL
                     IGAPPGYVGYDEGGQLTEAVRRSPYSIILFDEIEKAHPEVFNLLLQLLDDGRLTDSQG
                     RVVNFKNTVIIMTSNLGSEFFLKNQTSKIDDLVKATFKPEFLNRIDEIVYFLPLSKDT
                     QRQITRKMLDELMAHVAKNNFKLIYDEKIVDFVLDNAYAPEFGARPLKRFIQKSLETF
                     IAEQIVQGNIDSSITYRLTVTNNEVSISPFA"
BASE COUNT          449 a          318 c          280 g          493 t
ORIGIN      
        1 tgctagtttg gattgcggga aaacgtgctc acgattccat ttaccgtagt tatacccagc
       61 gtgcggatga ctacgtaagt catacactga taatacatta cctggtttat ttggatcagc
      121 gtccattact tttattgcgt cataagcaac attgtatgta actctattat ctaaaacacc
      181 tggcacataa aacttcgacc acatgtaatc ggcaagtgct tgacgaagct cttcaccgcg
      241 gagtgtaaag tcaatcgtgt tccaaaaatt accagtgtaa gcaggtagtg gtctaaaatt
      301 ccaattttca gctggtttta atactggttc aggttcaacc cattcagaag acacctcaac
      361 acttgattct tcaacactcg taacttcttc agttgtagat gtttcactga cgatacttgg
      421 tgtatcacta atgtcactaa cgcttggaat ttcactctca ggtacttcaa tactctgtga
      481 tgtaggtggt tcacacgacg ctaaaagcgt aactccgatc agcgctaata ataatcgacg
      541 tattttcata attaaaagac ctcttttctt atgtgattta attataacaa agaggccttt
      601 ttttgtaaat taaagttagg caaagggcga aatactaact tcgttatttg ttaccgttaa
      661 gcgataagta atactgctat cgatattgcc ctgaacaatt tgttcagcga taaatgtttc
      721 taaactcttt tggataaagc gttttaatgg tcgcgcccca aattctggag cgtaagcatt
      781 atcaagcaca aagtcgacaa tcttttcgtc atatattaac ttgaagttat ttttggcaac
      841 atgagccatt agttcatcaa gcatcttgcg cgtgatttgt cgttgtgtgt ctttactaag
      901 cggtaagaaa tacacaattt catcaatccg atttaagaat tcgggtttaa aagtcgcttt
      961 cactaagtca tcaatctttg atgtttgatt tttaaggaag aattcactac ctaagttact
     1021 cgtcataata atgacagtat ttttaaagtt tacaaccctt ccttgagaat cagttaagcg
     1081 gccatcatct agtagttgta agagtaaatt aaagacttca ggatgcgcct tttcgatttc
     1141 atcaaataat ataattgaat aaggactacg ccgcactgct tcggttaatt gcccgccctc
     1201 atcataacca acgtatcccg gtggggcacc aataagacgc gagacactaa atttttccat
     1261 atattcactc atatcaatcc ggacaatatg gcgttcgtcg tcaaaaagag cgtaggctaa
     1321 gcttttggct acttcggtct taccaacccc tgttgggcct aggaataaga aactaccaat
     1381 tgggcgattt gggttattaa gtcctgcgcg ttgccgtaaa atggcatcac taacaagccg
     1441 cagggctaca tcttgcccta atacgcgttt ttttagttca tcataaagcg ataaaatctt
     1501 ttctttttca ctttccatga tccgttttaa aggaatgcct
//
LOCUS       DAOSEX010000223         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_84545_length_1540_cov_2.323232, whole genome shotgun sequence.
ACCESSION   DAOSEX010000223 DAOSEX010000000
VERSION     DAOSEX010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84545_length_1540_cov_2.323232"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="PK612_04120"
     CDS             complement(<1..144)
                     /locus_tag="PK612_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOH95324.1"
                     /translation="MRVVIQIVKDAKLEIDNKVYSSIDYGMLLLVSFTLSDTKEVAKK
                     MAEK"
     gene            complement(144..1451)
                     /locus_tag="PK612_04125"
     CDS             complement(144..1451)
                     /locus_tag="PK612_04125"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOH95325.1"
                     /translation="MRMYDIILKKRDGFALTEEEIKFFIKGVTDGTIPDYQTSSLLMA
                     IVWRGMNKEETAALTLAMMHSGDVIDLSAIKGVKVDKHSTGGVGDKTSLVLGPLVASF
                     GVKVAKMSGRGLGHTGGTLDKLESIPGTCIDIPNDDFIRQVNEVGCAIIGQTGHLVPA
                     DKKLYALRDVTATVEAIPLIASSIMSKKLASGSDTILLDVKFGSGAFMKTLKDAQELA
                     RAMVDIGKSLNRDTRAILTDMDQPLGLAVGNALEVKEAIATLKGEGPRDLSELCIEAA
                     CIMLEQAGVVKKAQDARKEVENKIKSGAALQKLVDMVKAQGGDESYITNPEKFAISQN
                     VFEVLATSEGYVEHIDSMAIGISSMKLGGGRETYEDEIDMSAGIVLNKKVGDKVKVGE
                     RLALVYTNKADEVSTPILVDIHHAFKLSDKHIDVPPIIHDYIK"
BASE COUNT          453 a          365 c          225 g          497 t
ORIGIN      
        1 cttttccgcc atttttttcg ctacttcttt agtgtcacta agcgtaaaag atacaagtaa
       61 aagcattccg taatcaatac ttgaatacac tttattatct atttctaatt tagcatcttt
      121 aacaatttga ataacaacac gcattactta atataatcat gaataattgg tgggacatca
      181 atatgcttat cgctaagttt aaaggcatga tgaatatcaa ctaagattgg agtagaaact
      241 tcatcagctt tattcgtata tacgagcgct aggcgttcac caactttcac tttatctcct
      301 acttttttat ttaaaacaat tccggcggac atatcaatct catcttcata agtttcgcgg
      361 ccaccaccta atttcatact agaaatacca attgccatac tatcaatatg ttcaacataa
      421 ccttcactag tcgctaatac ttcaaacaca ttttgactaa tcgcaaattt ttccgggtta
      481 gtaatataac tctcgtcacc accttgtgct ttcaccatat cgacaagttt ttggagcgca
      541 gcacctgact taattttatt ttcaacttct ttgcgagcgt cttgtgcttt ttttacgaca
      601 cctgcttgtt caagcataat acacgctgct tcgatacata attcacttaa gtcacgcggt
      661 ccttcgcctt taagcgtggc gattgcttct ttgacttcaa gtgcattacc gactgcaagg
      721 cctagtggtt gatccatatc cgttaaaata gcccgtgtat cacggtttaa actcttacca
      781 atatcaacca tcgctcgcgc taattcttgg gcgtctttta gtgtcttcat aaacgcaccg
      841 ctaccaaatt taacatcaag taaaatcgtg tcactgccgg aagcaagttt ctttgacata
      901 atggaactag caataagcgg aatagcttca acagttgctg tcacatcacg aagcgcatat
      961 aacttcttat cagcgggtac taagtgcccc gtttgcccaa taatcgcaca gcctacttca
     1021 ttaacttgcc gaataaagtc atcatttgga atatcaatac acgtacctgg aatactttca
     1081 agtttatcaa gtgtcccgcc ggtatgccct aagccgcgcc cactcatttt ggcgacttta
     1141 acaccaaaac tagcgacaag cggaccaagc actaagcttg ttttatcacc aacaccaccc
     1201 gtgctatgtt tatcaacttt aacaccttta atggcactta aatcaataac atcaccacta
     1261 tgcatcatcg ctagtgttaa ggcggcggtt tcttctttat tcattccgcg ccacacaatc
     1321 gccattagta aactactcgt ttgataatca ggaatagtcc cgtcagttac tcctttaata
     1381 aagaatttaa tttcttcttc agtaagtgcg aaaccatcgc gttttttcaa aataatgtca
     1441 tacatccgca tagttaatat ctcctttatt aaaactattt taccatacta aaaagcgctt
     1501 actctactaa gatagagtaa actatacttt ttatcgtcat
//
LOCUS       DAOSEX010000224         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_84854_length_1536_cov_3.526671, whole genome shotgun sequence.
ACCESSION   DAOSEX010000224 DAOSEX010000000
VERSION     DAOSEX010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84854_length_1536_cov_3.526671"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..603)
                     /locus_tag="PK612_04130"
     CDS             complement(166..603)
                     /locus_tag="PK612_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HOH95326.1"
                     /translation="MSKLHNGLNKQVANLAVFFVKLHHHHWYIKGQHFYGLHAKFEEF
                     YDEVNELYDAVAERLLMIGGKPYSTMKDYLANSSLVEASGGETATEMVTAIKQDFKTL
                     RDEFNALIKVAQDEGDEVTTDLLLGAVTSFDKHIWMLNATLGK"
     gene            1023..>1536
                     /locus_tag="PK612_04135"
     CDS             1023..>1536
                     /locus_tag="PK612_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95327.1"
                     /translation="MKTRKLKIFALALTLPLLTGCKLIHNFICGIALNVAFNSPDLLS
                     KVNVVEGGAKLTPIEDKPTSADKRASLTYGIEALVLPKRIETVQYGYEIIIDFTFTFS
                     EGAQDYFYIRDVEDAQEGEYNVDGTILYPTGTVENPSNEQEISDWLTVWRMFDLKNQE
                     SKDITITLTGK"
BASE COUNT          506 a          272 c          240 g          518 t
ORIGIN      
        1 acccccgacc ttctgtacgt caaacagatg ctctcccagc tgagctaaac atccagccac
       61 ataagtataa caaagcgatt aactttgtca attcatataa ttgcgattta taaaaaagaa
      121 aaccccagtt tgaaatatat tgaactgggg ttgtttcgct aaaatttact tacctaatgt
      181 agcgttaagc atccatatgt gcttatcaaa tgatgtaaca gcaccaagta gtaagtcagt
      241 tgttacttca tcaccttcat cttgtgctac tttaattagt gcattaaatt catctcttaa
      301 ggttttaaag tcttgtttaa ttgctgttac catttccgta gcagtttcac cgccactagc
      361 ttcaacaagc gaactattag cgagataatc tttcattgtc gaatatggtt taccaccaat
      421 cattaaaaga cgttcggcta cagcatcgta aagttcatta acttcatcat aaaattcttc
      481 gaatttagca tgtaatccgt aaaaatgttg acctttaata taccagtggt ggtgatgtaa
      541 cttaacaaaa aacacagcta agtttgcaac ttgtttattt aagccgttat gtaattttga
      601 cataattttt cctccttttt tcttcaatac acattatacc attatttgaa aatgttacct
      661 aaaatgatgc tccctttaaa aaatatcaac atataatcgc tattatcaca tattataagt
      721 tttatttatg gggtttgagt gtttttattg cttttattta taattggtgt ataatgagcc
      781 tgtaagtaag ttaacttact tgccgccccc ctaaaagaat agcaacgtta aggaggtgaa
      841 agtatgcgta aattcttaaa taaactagcg aagttactgc ttgatcaagt aatcttcagc
      901 aataacaaat aatcttgcac accttgtgta agattttttt tgctctttta tcacttaatc
      961 aatataaata ctcctaacaa gatttcttgt tttaaagtac aatggataca ggaggatttg
     1021 ttatgaaaac tcgtaaactt aaaatattcg cactcgcact tacactacct ttactaactg
     1081 ggtgtaagtt aatccataat ttcatttgtg gcattgcttt aaatgtcgct tttaattcac
     1141 cagatttatt aagcaaagtt aatgttgtag aaggtggtgc aaagttaaca ccaattgaag
     1201 ataaaccaac ttccgcagat aaacgcgcga gtttaactta tggtatcgaa gcactcgttc
     1261 ttcctaaacg aatcgaaact gttcaatatg gttatgaaat aatcattgac tttactttta
     1321 ctttcagtga aggagcgcaa gattatttct atattcgtga tgtcgaagac gcacaagaag
     1381 gtgaatataa tgttgatggt actattctct atccaactgg cactgttgaa aatccgagta
     1441 acgaacaaga aataagtgat tggctaaccg tatggcggat gtttgattta aaaaaccaag
     1501 aatcaaaaga cattactatt actttaacag gtaagt
//
LOCUS       DAOSEX010000225         1530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_85437_length_1530_cov_2.226441, whole genome shotgun sequence.
ACCESSION   DAOSEX010000225 DAOSEX010000000
VERSION     DAOSEX010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85437_length_1530_cov_2.226441"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="PK612_04140"
     CDS             complement(<1..1516)
                     /locus_tag="PK612_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOH95328.1"
                     /translation="MFAECDNLKHVDFGNGVQEIGFMAFDHCPSLEAIALPDSVETIY
                     GEAFANCPSLTSVRMSANIKTFGLGVFNKSPNVEYKVYENGLYLGNSTLSEYLVFVEI
                     VDKTQTTLKLHPDTRLIAEFACYSSDVAEITSWGDNLRTINYGSFMRSKLTAITLPDT
                     LEEIGDGAFKQTKLTSLKFGTGLKAIGYEAFSNCTDLADVTLPNSLEQIDSSVFYGTS
                     VTYTDYENGRYLGSGGTGETANPYFALMEVITKTMTTFTVHADTHLIVPGVFYGSYSL
                     ESVEIKEKVSFIGSGAFENCIALTSLSVAANNTHFYSPSNKAILEKDTDRLLFGLNGG
                     FIPENTKIIGEKAFYYRKFTEPLVIPSSVTTIEDYAFYQTNVTAIVIPDSVTKIGKSV
                     FQYSNSLTSVTLSKNLKVIDEWTFSGCEIIETLTIPDSVTMIKEWAFSNCDMLANVTY
                     SANLAFIGDVAFSSCIALTTLPASESLQVFGSGSFRGCDSLTSITIPKNVRYISS"
BASE COUNT          504 a          311 c          234 g          481 t
ORIGIN      
        1 cgctagaaat atagcgtaca tttttaggaa tcgtaatact tgttaaacta tcacaccctc
       61 taaatgaccc actgccaaac acttgcaaac tttcagatgc tggaagggta gttaaggcaa
      121 tacaacttga aaaagcaaca tccccaataa acgctaaatt agcgctataa gtaacattag
      181 caagcatatc acaattacta aatgcccact ctttaatcat tgtgactgaa tcaggtatag
      241 taagtgtttc tattatttca caaccactaa aagtccattc atcaataact tttaagttct
      301 tacttaaagt tactgaagta aggctattac tatattggaa cactgatttt ccaattttag
      361 tgacactatc aggaatgaca atcgctgtta catttgtttg ataaaaagca taatcttcaa
      421 ttgtcgtgac actacttgga atgactaaag gctcagtaaa ttttcggtaa taaaaggctt
      481 tttcaccaat gatttttgta ttttctggaa taaaaccacc atttagacca aatagtaaac
      541 gatctgtgtc tttttctaaa attgctttat tcgatggtga gtaaaaatgc gtgttattag
      601 cggcaacact aagagaagtt aacgcaatac aattctcaaa cgctccgcta ccaataaatg
      661 acaccttctc tttaatttcc acactttcaa gactgtaaga tccataaaaa acacccggca
      721 caataagatg cgtatcagcg tgaacagtaa atgtcgtcat cgtttttgtt attacttcca
      781 ttaaggcaaa atacggatta gcggtttctc cggtgccgcc gcttcctaaa tatcgtccgt
      841 tttcgtaatc agtataggta acgctggtgc cataaaaaac acttgaatct atttgttcta
      901 aactattagg caatgtaaca tccgctaaat cggtacaatt actaaacgcc tcataaccaa
      961 tcgctttgag tcctgtgcca aattttaggc ttgttagttt tgtttgttta aaagcaccat
     1021 caccaatttc ttctaatgtg tcaggtagtg tgattgctgt taattttgaa cgcataaagg
     1081 aaccgtaatt aattgtccgt aaattatcac cccacgatgt tatttcagca acatcgcttg
     1141 aataacaggc aaattccgca attaggcgcg tatcaggatg taatttaagt gttgtttgtg
     1201 ttttatccac tatctccaca aagactaaat attcgctaag tgtactattc ccaagatata
     1261 agccattttc ataaacctta tattcaacat tcggagactt attaaaaacg cctagcccaa
     1321 aagttttaat attcgctgac atacgaacgc ttgttaatga tggacaatta gcgaaagctt
     1381 caccgtaaat tgtctcaaca ctatctggta aagcaattgc ttctaaactt ggacaatgat
     1441 caaatgccat aaagccaatt tcttgtacgc cattaccaaa atcaacatgt tttaaattat
     1501 cacactcggc aaacacatct tcaccgattt
//
LOCUS       DAOSEX010000226         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_86360_length_1519_cov_3.197404, whole genome shotgun sequence.
ACCESSION   DAOSEX010000226 DAOSEX010000000
VERSION     DAOSEX010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86360_length_1519_cov_3.197404"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PK612_04145"
     CDS             <1..700
                     /locus_tag="PK612_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95329.1"
                     /translation="LIAAPIIIGAKLFNAGKSNYQLAAMLGWGNLHILSRTLINTIDD
                     LSGKSGLAFAEHSTPGIMFKLATSDVSDINVNYVKAVSDVGGPFIQKQYDYVLISEPV
                     LSKIRSNINDETYTFSLQNVAKLPKIAQFGLFSNPATKAKDVNKFLVKLKNNVEQLNT
                     DPESYVDSIIDSDDYFKGLGRDVLIDAIPRLDIEYKSALEAKADLTLFFDYLISVDTN
                     LIGATSLDESFFRS"
     gene            678..1442
                     /locus_tag="PK612_04150"
     CDS             678..1442
                     /locus_tag="PK612_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95330.1"
                     /translation="MKASSVPKKKPTAQIIASSLGFLTIILLWIIISALHQNDIIFPG
                     IISTLKVFFSLFQRGSTYFILLNTIGGLILVLVLSFLTALILSLISLKFPTFKSYITP
                     FLALFKIIPVPALLIVFLVDFKQSFIPFLLTYIVLLPLVYDGLYGAFISINKDLIDEL
                     KITSKLNLLMIFKIYIPIVSKQVISTLLQALGLGLKVKVMSEFIAEAPNTIGYELSYG
                     RATLSMDIVFAWSLILIVIVVILDAVLVNYLGKQEA"
BASE COUNT          495 a          217 c          246 g          561 t
ORIGIN      
        1 tttaatcgct gcaccgatta ttattggcgc aaagttattc aacgctggta aatcgaacta
       61 tcaactcgcc gcaatgctcg gctggggtaa cttacatatt ttaagtcgga cactaattaa
      121 tactattgat gacttaagcg ggaaaagtgg tttagctttt gctgaacatt caacaccagg
      181 cattatgttt aaattagcga caagtgatgt tagtgatatt aatgtaaatt atgttaaagc
      241 agttagtgat gtcggcggac catttatcca aaaacaatat gattatgttt taattagtga
      301 accagtttta agtaagatta gaagtaatat aaatgatgaa acatatacat tttccctgca
      361 aaacgttgct aaattaccta aaatagcgca gtttggatta tttagtaatc ccgctactaa
      421 agcaaaagat gtaaataaat ttttagttaa acttaaaaac aatgttgaac aacttaacac
      481 tgatccagaa agttatgttg atagtattat tgacagtgat gactacttta agggactcgg
      541 tcgagatgtt ttaattgatg ctattccccg tttagatatt gaatacaaaa gtgcgcttga
      601 agcaaaagca gatcttactc tcttttttga ttatttaatt agtgttgaca ctaacttaat
      661 aggagcgacg agtcttgatg aaagcttctt ccgttcctaa gaaaaaacca acagcgcaaa
      721 ttattgctag ctcattaggc tttttgacaa tcattttact atggattatt attagtgcgc
      781 tgcaccaaaa tgatattata ttccccggaa ttattagtac tttaaaagtg tttttttctt
      841 tattccaaag agggtctaca tattttattt tactaaacac tattggggga ttaattctcg
      901 ttttagtttt atcattttta acggcattaa ttttaagttt aatatcgtta aaatttccaa
      961 cttttaaaag ttatattaca ccctttttag cactatttaa aattatccct gttccagcgc
     1021 ttttaattgt gtttttagtt gattttaaac aaagctttat tccgttttta ctaacatata
     1081 ttgtgctttt accacttgtt tatgatggat tatatggggc ttttattagc attaataaag
     1141 atttgattga tgaattaaaa attacttcaa aacttaattt attaatgatt tttaaaatat
     1201 acattccgat tgtaagcaaa caagttattt cgactttatt acaagcctta gggctaggac
     1261 ttaaagtaaa agttatgagc gaatttatcg ctgaagcgcc taatacgatt ggttacgaac
     1321 tctcttatgg acgggcaact ttatcgatgg atattgtatt tgcgtggtca cttattttaa
     1381 tcgttatcgt cgttatttta gatgctgttt tagtgaatta tttaggtaaa caagaagctt
     1441 aaatttttta atagatatta aaaaaacact gtttttcaca taattttttc gcaataatat
     1501 ataataagac aagtagagg
//
LOCUS       DAOSEX010000227         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_86461_length_1518_cov_2.855092, whole genome shotgun sequence.
ACCESSION   DAOSEX010000227 DAOSEX010000000
VERSION     DAOSEX010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86461_length_1518_cov_2.855092"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PK612_04155"
     CDS             <1..492
                     /locus_tag="PK612_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95331.1"
                     /translation="ILKILEGLKYDGRAQDLLRLSMWYLQITYSAPNISAALRYAGYA
                     LYLDGKFAEAYAFYYQAIVYQDGETPARFNEEITSVLTALKKDAPYELSQAQIKKLFA
                     YSKFKPYPSEIAFDTIRPLIINHYTKGNYVDAIRYATQYVIFRPNDDKIMRILKASKL
                     NLD"
     gene            602..>1518
                     /locus_tag="PK612_04160"
     CDS             602..>1518
                     /locus_tag="PK612_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH95332.1"
                     /translation="MNFNPFPNYEQDEKILEKFGRFLDEDAKLNKSDPVIGRDSEIRR
                     IIEILARKQKNNVILLGEPGVGKTAIIEGLAQRIVAGDVPKNLADKRIYELDMGALVA
                     GAKFRGEFEERLKAILKKIKESNHQIILFIDEIHLIVGAGKAEGAIDAGNMLKPMLAR
                     GEIHTIGATTLDEYRTYIEKDRALERRFQPLIVQEPTLEDTISILRGLKARYEAHHGV
                     KISDNALVSAAVLSDRYITERFLPDKALDLVDEACALIRVEVESLPAELDEIRRKMRQ
                     LEIERVALSKENDDLSKVRLQKVNEELENL"
BASE COUNT          520 a          248 c          315 g          435 t
ORIGIN      
        1 attcttaaaa tccttgaagg tttaaagtat gatggtcgcg ctcaagattt attacggctt
       61 tcaatgtggt acttgcaaat tacttatagt gccccaaaca ttagtgcagc attgcgttat
      121 gcaggatatg cgctttatct tgatggtaag ttcgccgaag cttatgcctt ttattaccaa
      181 gcaatcgtgt atcaagatgg tgaaacacca gcacgtttta atgaagaaat tacaagtgta
      241 ttaactgcct tgaaaaaaga tgccccatac gaattaagcc aggcacaaat taaaaaactt
      301 tttgcgtatt caaaatttaa accgtatcct agtgaaatag catttgatac gattagacca
      361 ctaattatta atcattatac aaaaggtaat tatgttgatg ctattcgtta tgcgacacag
      421 tatgttattt tccgtccaaa tgatgataag ataatgcgca ttttaaaagc aagtaaactt
      481 aatttagact aataatatta aaaatattac aaaaaatggc actcggtgtt gacaagtgcc
      541 aattttagta tataatagac ttagcactca gacatttaaa gtgctaagga ggtgttatat
      601 tatgaatttt aatccttttc caaattatga acaagatgaa aaaatcttag agaagtttgg
      661 acgcttttta gatgaagacg ctaaactaaa taaaagtgac ccagtaattg ggcgtgatag
      721 tgaaattaga cgcattattg aaattttagc gagaaagcaa aaaaataatg ttattttact
      781 tggtgaacca ggggtcggta agaccgctat tattgaagga ctagcgcaac gaatcgttgc
      841 tggtgatgtg ccaaagaatt tagcagataa aagaatctat gaattagata tgggcgcact
      901 agtggctggt gcgaagttcc gcggtgaatt tgaagagcgg ctcaaagcca ttttaaagaa
      961 aattaaagaa agtaatcatc aaatcatttt atttattgat gagattcatt taatcgtcgg
     1021 cgctggtaaa gccgaaggag ccattgacgc tggtaatatg cttaaaccaa tgttagcgcg
     1081 cggtgaaatt catacgattg gggcgacgac acttgacgaa tatcgcactt acattgaaaa
     1141 agaccgagcg cttgaaagaa gattccaacc gcttattgtc caagaaccga cgcttgaaga
     1201 tacaatttcg atattacgcg gactaaaagc gcgttacgaa gcccatcacg gcgttaagat
     1261 aagcgataat gcacttgtga gtgcagcagt acttagtgac cgttatatta ccgaacgctt
     1321 tttacccgac aaggcgcttg acttagtgga cgaagcttgt gcattaattc gtgttgaagt
     1381 tgaaagttta cccgccgaac tcgatgaaat tcgccggaag atgcggcaac ttgaaattga
     1441 acgagttgca cttagtaaag aaaatgatga cttaagtaaa gttcgtttac aaaaagtaaa
     1501 tgaagaactg gaaaatct
//
LOCUS       DAOSEX010000228         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_86576_length_1517_cov_1.989056, whole genome shotgun sequence.
ACCESSION   DAOSEX010000228 DAOSEX010000000
VERSION     DAOSEX010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86576_length_1517_cov_1.989056"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..262
                     /locus_tag="PK612_04165"
     CDS             65..262
                     /locus_tag="PK612_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95333.1"
                     /translation="MEISIRELKKAKKGDLLVCTGDGKFEIASLDELLVDRDKRLAKC
                     EELKSELNTLRLQIAKALKEI"
     gene            267..767
                     /locus_tag="PK612_04170"
     CDS             267..767
                     /locus_tag="PK612_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95334.1"
                     /translation="MDEIMNWFATFDFEKFTILMTWLASSGILTWAGIIWSKYRKYKA
                     LTPTEIKDDIMSELSPLIESKIDEVITIAVDKALSPILEYLKNIEAIAKVQVESLVLS
                     NSTDPQSKLALIENVSKVKGIKTVVIEKVKEEVKEFVAEQAEAQKRKEQAIADIEQSV
                     DYTETL"
     gene            896..1234
                     /locus_tag="PK612_04175"
     CDS             896..1234
                     /locus_tag="PK612_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95335.1"
                     /translation="MMGGWSLILIAIVSIPIFYALSRIVKALEQCLVKQIIVGVMYVI
                     VPLVIVLFMTSVIAENVMKIQRILYIVIPSVAIGIVVNPLPAWVAEREQTQIEQAMRV
                     AFAKKPKGKK"
     gene            1305..>1517
                     /locus_tag="PK612_04180"
     CDS             1305..>1517
                     /locus_tag="PK612_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95336.1"
                     /translation="MKPVKVGRARVVITSLLTFLMVALLAFTLLLQVRFDIKQFNWFE
                     FTFSIINFVAGRGIYFPMGLDVGMAQP"
BASE COUNT          483 a          237 c          341 g          456 t
ORIGIN      
        1 aaatggtgtc aatacattta gttggatatt agataatagt taaggaaagg tggtaatgaa
       61 caatatggaa attagtatta gggaattaaa gaaagcgaaa aagggcgatt tgctcgtttg
      121 cactggcgat ggcaagtttg aaatcgcatc gcttgatgag ttgttagttg atcgtgataa
      181 gcgattggcg aaatgcgaag aattgaaaag tgaattaaac actttgagat tacaaattgc
      241 gaaagcatta aaggagatct aattgtatgg atgaaattat gaattggttt gcaacattcg
      301 attttgagaa gtttaccatc ttgatgactt ggttggcttc gagtggcatt ttaacttggg
      361 ctggaatcat atggtctaaa tatcgcaagt ataaagcact gacaccaacc gaaatcaaag
      421 atgacattat gagcgagtta tcgccactca tcgaaagcaa gattgatgaa gttattacta
      481 ttgcggtcga taaggcatta tcgcctatcc ttgaatattt gaagaacatt gaagccattg
      541 ccaaagtcca ggtcgaaagt ttagtgttat caaatagcac tgatccacaa tctaaacttg
      601 ccttgatcga aaatgtatca aaggtcaaag gcattaagac cgttgtaatc gaaaaggtaa
      661 aagaagaagt taaagagttt gtcgcggaac aagccgaagc acaaaagcgc aaagagcaag
      721 ccatcgccga tatcgagcaa tcagtagatt ataccgaaac gctttaaggt aatttatcat
      781 gagcgataga acgaagtttt ggattaagtt aatcgcattt atgtttttta cctgcatagt
      841 gccttttctt gctataaatt attactatga tctatttaat gtcgaggggc ggtttatgat
      901 gggcggttgg tcgttaattt taatcgctat cgtgtccata ccgatattct atgccctatc
      961 acgcattgtt aaagcgttag agcaatgttt agtcaaacaa atcatcgttg gcgtaatgta
     1021 tgtcatcgtg ccactcgtta tcgttttatt tatgacaagt gtaatcgctg aaaatgtaat
     1081 gaaaatacaa cgcatacttt atatcgtgat accaagcgtc gccattggca tcgtggttaa
     1141 tccattaccg gcttgggttg ccgagcgcga acaaacgcaa atcgaacaag caatgcgcgt
     1201 agcgttcgct aaaaagccaa aaggcaagaa ataactaata ataaagatat tgtgttgtat
     1261 agtaaaataa aggggaaaat tgttttatga ataaaaggtt gaatatgaag cccgtaaaag
     1321 tcgggcgtgc gagggttgtt attacctcgc ttttaacatt cttgatggtc gcgttgctgg
     1381 cctttacttt gttattgcaa gtaaggtttg acattaagca gtttaattgg tttgagttta
     1441 cattcagcat cattaacttc gttgctggtc gcggtattta tttcccgatg ggtttagatg
     1501 ttggaatggc acaacca
//
LOCUS       DAOSEX010000229         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_86956_length_1512_cov_3.839396, whole genome shotgun sequence.
ACCESSION   DAOSEX010000229 DAOSEX010000000
VERSION     DAOSEX010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86956_length_1512_cov_3.839396"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PK612_04185"
     CDS             complement(<1..653)
                     /locus_tag="PK612_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOH95337.1"
                     /translation="MRYLGLTMTELHELYVSGKATPLDVVNEVIEALTKDKNNILEKT
                     MYDEARSMAQSLTKVEKDNLLWGIPFLVKDNIAMKGIETTGSSNILNGFIPLCNAEVI
                     DKLLAAKAIPVAKTTLDEFGMGGRGETGHKGITYNPYGKERTRIVGGSSSGSASGVAS
                     GYVPFAVGSDTGDSIRKPASFAGLVGFKPTWGEISRFGLFAFMPTLDHVGFFTRSVS"
     gene            complement(655..942)
                     /locus_tag="PK612_04190"
     CDS             complement(655..942)
                     /locus_tag="PK612_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95338.1"
                     /translation="MNKEEIRKLAAEILITLTPEEEATFAAEFAYFKELAKLLEALPN
                     IDEKEPMVFPFKVTQTYLKEDTPEESPGAKEILKNSKNAKDAFVVIPKVVK"
     gene            complement(965..>1512)
                     /locus_tag="PK612_04195"
     CDS             complement(965..>1512)
                     /locus_tag="PK612_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOH95339.1"
                     /translation="EKSVDNLLNAIEKSKEKSLEKLVFGLGIKELGAKTSKILAEKYM
                     TLDALSCATEAELLTIRDIGPVAASSIVSYFSNEQNLALITKLKEFGLNTDYLGSVKK
                     DNTFFSGKTIVITGKFENYSRDELTALLENLGAKVAGSVSKKTSLVVAGEDAGSKLTK
                     AQELNIDILNEEQLLKLISEA"
BASE COUNT          430 a          318 c          230 g          534 t
ORIGIN      
        1 tcactaacac tgcgggtaaa aaatccaaca tggtctaaag ttggcataaa tgcgaataga
       61 ccaaaacgcg aaatttcgcc ccatgttggt ttaaagccaa cgagtccagc aaagcttgcg
      121 ggcttacgga ttgagtctcc cgtatcacta ccaaccgcaa aaggaacata cccactagcg
      181 accccactag cgctaccgct actagaacca ccgacaattc gtgttctttc tttaccatac
      241 ggattatatg ttatcccttt gtgaccagtt tcaccgcggc cacccatccc aaattcatca
      301 agtgttgttt ttgcaacagg aattgcttta gcagctaaaa gtttatcaat aacttcagca
      361 ttgcaaagcg gtataaagcc gttaagtata ttactactgc ctgttgtttc aatgcctttc
      421 attgcgatat tatctttgac taaaaaagga attccccata gtaaattatc tttttcaact
      481 ttagtaagac tttgcgccat actgcgtgct tcgtcataca ttgttttttc taaaatgttg
      541 tttttatctt ttgttaaggc ttcaattact tcattaacaa catctaatgg tgttgcttta
      601 cccgacacat ataattcatg aagttccgtc attgttaaac ctaggtagcg catattattt
      661 caccaccttt gggataacga caaaagcgtc ttttgcgttt ttactatttt ttaatatctc
      721 tttagcgcca ggactttctt ccggcgtgtc ttcttttaaa tatgtttgtg ttactttaaa
      781 agggaacacc attggttctt tttcatcaat attaggtaaa gcttctaaaa gcttcgctaa
      841 ttctttaaag taagcaaatt ctgcggcaaa agtggcttct tcttcaggag taagcgttat
      901 taatatctct gctgctaact ttcttatttc ttctttattc ataattatcc tcgctagtaa
      961 tatatcatgc ttcgctaatt aattttagca attgttcttc gtttaatata tcaatattaa
     1021 gttcttgcgc ttttgttaat ttgctaccag cgtcttcgcc agcaacaaca agtgaagttt
     1081 ttttactaac actgcccgct actttagcgc ctaaattttc taagagagcg gttaattcgt
     1141 cacgtgaata attttcaaac ttacccgtaa tgacaattgt cttaccagag aagaaagtat
     1201 tatctttttt aacggaccct agataatccg tgtttaagcc gaactctttt agtttagtaa
     1261 ttaacgctaa attttgttca ttactaaagt aagacacaat tgaagaagca gcaactggac
     1321 cgatatctct aattgttaaa agttctgctt cagtagcgca actaagtgcg tctaaagtca
     1381 tatacttctc ggctagaatc ttacttgttt tcgctcctaa ttctttaatc cctaaaccaa
     1441 acactaattt ttctaaagac ttttctttac ttttttcaat agcgtttaat aagttatcaa
     1501 ctgatttttc ac
//
LOCUS       DAOSEX010000230         1498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_88193_length_1498_cov_2.687457, whole genome shotgun sequence.
ACCESSION   DAOSEX010000230 DAOSEX010000000
VERSION     DAOSEX010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88193_length_1498_cov_2.687457"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..316)
                     /locus_tag="PK612_04200"
     CDS             complement(50..316)
                     /locus_tag="PK612_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95340.1"
                     /translation="MQINVTHKFEKVFAYTKDFKSPLTKMQKMMLTELERAKDPRKIT
                     KTFLKAIYKKRPKTVYKVNNSFKMKLLNALPVKVQDFILGLFFK"
     gene            complement(345..857)
                     /locus_tag="PK612_04205"
     CDS             complement(345..857)
                     /locus_tag="PK612_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH95341.1"
                     /translation="MKYALITGASGGLAHNIITAIKDDFFLILTDINPAISDKYNDLP
                     NKLIVVSDLTDKDSINNLFDKILKITDKLDLVIHFAGILEIGPVMEVKVEALETLMQV
                     NLFAVYFINQKAFPLLKKAKGRIIHLSSEYGTLLALPFHSFYTIAKRSIEAYNDSLRR
                     EITKFWHQGY"
     gene            977..>1498
                     /locus_tag="PK612_04210"
     CDS             977..>1498
                     /locus_tag="PK612_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HOH95342.1"
                     /translation="MNVFVYIFFGLFIAVTIAELIMAFNEIEKARMILKPFSMFFLAI
                     AALIAVPNHPLIYLGAFLGMVGDIFLLWPKNKKMFLAGATSFLVGHTLYISEIIHIMF
                     KGQALPWWFYAVVSFGVLLFVLAFYPMSKKICGDRYLTLVGNTYIAVLILVSVVSIIA
                     CVKGFTNFMVLGII"
BASE COUNT          446 a          210 c          274 g          568 t
ORIGIN      
        1 aaaaagaaaa agtagcgagg tgctactttt ttcttaaata gtattttaat tatttaaaaa
       61 atagtcctaa aataaaatct tgaactttaa cgggaagagc atttaataat ttcatcttaa
      121 acgaattatt tactttataa acagtttttg gtcttttctt atatattgct tttaaaaatg
      181 ttttagtgat tttacggggg tctttagctc tttctaactc ggtaagcatc atcttttgca
      241 tttttgtaag cgggctttta aagtctttag tgtaggcaaa tactttttca aatttatgcg
      301 taacattaat ttgcatatct gttttaaaag caccgggtct aactttaata accttgatgc
      361 caaaacttcg taatttctcg tcgtaaacta tcgttatatg cttcaatgct gcgttttgct
      421 atcgtgtaga aggagtggaa gggtagtgct aaaagtgtgc cgtactcact acttaaatga
      481 ataatgcgac ctttagcttt ttttaagagc gggaaggctt tttggttaat aaagtagacc
      541 gcaaaaagat taacttgcat taaggtttca agtgcttcaa cctttacttc cattactgga
      601 ccaatctcta aaataccggc gaagtgaatg actaaatcaa gcttgtctgt aattttaagt
      661 atcttatcaa aaagattatt tattgagtct ttatctgtta agtcgctaac cacaataagt
      721 ttatttggta aatcattata cttatcactt attgctggat taatatcagt gagtattaaa
      781 aagaagtcat ctttgatggc agtgataatg ttgtgcgcta agccaccact agcgccagta
      841 ataagcgcat atttcataaa tttattttaa cactctaaat gtaaatgagt gtcaatattt
      901 ggaaaatagc ggtaaattca ctacttttta ataatattaa aagatgataa tataaaaggg
      961 taggagaaat atagttatga acgtatttgt gtatatcttt tttggtttat tcattgcggt
     1021 gacaattgct gaactaatta tggcttttaa tgagattgaa aaagcgcgga tgattcttaa
     1081 acctttttca atgttctttt tagcaattgc ggcacttatt gctgtaccta atcacccgct
     1141 tatttatctt ggagcatttt tagggatggt tggtgatatc ttcttactgt ggcctaaaaa
     1201 taagaagatg tttcttgcag gggcaacaag ctttttagtt ggacatacat tatatattag
     1261 tgaaatcatt cacattatgt ttaaaggtca agctttaccg tggtggtttt acgctgtggt
     1321 gagttttggc gtgttgctct ttgtgttagc gttttatcca atgtccaaaa agatttgtgg
     1381 tgaccggtat ttaacacttg ttggtaatac atatattgct gttttaattt tagtttctgt
     1441 tgtgagcatt attgcttgtg ttaaggggtt tactaacttt atggtactag gtatcatc
//
LOCUS       DAOSEX010000231         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_88315_length_1497_cov_2.298197, whole genome shotgun sequence.
ACCESSION   DAOSEX010000231 DAOSEX010000000
VERSION     DAOSEX010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88315_length_1497_cov_2.298197"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK612_04215"
     CDS             complement(<1..231)
                     /locus_tag="PK612_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95343.1"
                     /translation="MRNRKPKKQRIVKKRHQRYVNLANFLLGRLIRRSYGVKQAKIDI
                     KQYEPCLIVSNHILTFDPILMTSIFGHQIYYVA"
     gene            complement(221..1360)
                     /locus_tag="PK612_04220"
     CDS             complement(221..1360)
                     /locus_tag="PK612_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95344.1"
                     /translation="MIKIVPVYGGRKLRQFINFPVKLYKNSKQFTPYIFEDEISNLTP
                     SKNPASAWCDFKLFLAYRDRKIVGRICGIISHYANKKYDQKRVRFNRIDMIDDIEVTK
                     ALIKAVEEWGYSRGMNEIVGPLGYSDQDKEGLLVEGFEYSNMFATFYHFPYYMKHLEE
                     LGFVSDAVWVEHRIIIPKEIDERFERVSEMVKRKYNVKVRRLKSKRQIQPYVRKALAL
                     TNRAYDHLYGYVPMSEKLMDMLARQYVPLINLKYLILVVDEKDELLAFGLSIPSPVFA
                     LKKIKGKLFPFGFIRFLRALKKSKQLDLLLVAVEPELQNSGLANVIMYESMKSAIADG
                     IEYAETGPQLEYNKPVQRLWKHLNYFNHKRRICYVKPITEVEGEE"
BASE COUNT          482 a          276 c          236 g          503 t
ORIGIN      
        1 agcgacataa tatatttgat gaccaaaaat tgatgtcatt aaaatcggat caaaagtaag
       61 gatgtgatta ctgacaatta aacatggctc atattgctta atatcaatct ttgcttgttt
      121 aacaccataa gaacgacgaa ttaatctacc tagtaagaag ttagcgagat tgacataacg
      181 ttgatgacgt tttttaacaa tccgctgctt cttcggtttt ctattcctca ccttcaacct
      241 ccgtgatagg ttttacataa catatccggc gtttatgatt aaagtaattt agatgtttcc
      301 aaagccgttg gacaggttta ttatattcaa gttgcggacc agtttcagca tactcaatcc
      361 catcggcaat cgcgcttttc attgattcat acattattac attagcaaga ccactattct
      421 gcaattcagg ttcaaccgca acaaggagta agtctaattg ttttgatttc ttaagtgcgc
      481 gtaaaaagcg aataaatcca aacgggaata attttccttt aatcttttta agggcaaaaa
      541 caggagacgg aattgaaagc ccaaacgcta ataattcatc tttttcatcc actactaaaa
      601 taagatattt taagttaata agcgggacat attgtcgcgc tagcatatcc atgagttttt
      661 cactcattgg tacataacca taaagatggt cgtatgctct atttgttaac gctaaagctt
      721 tgcggacata tggttgaatt tgtcgttttg atttaagtct tctaactttg acattatatt
      781 tgcgttttac catttcacta acgcgttcaa aacgctcatc gatttctttc ggaataataa
      841 tgcggtgctc aacccataca gcatcactta caaatcctaa ttcctctaaa tgtttcatgt
      901 aatacgggaa gtgataaaat gtggcaaaca tatttgaata ttcaaaccct tccacaagta
      961 gtccttcttt atcttggtca ctataaccta aaggaccaac aatttcattc ataccgcgtg
     1021 aataacccca ttcttccact gctttaatga gtgcttttgt tacttcaata tcatcaatca
     1081 tatcgattcg attaaatcgg acacgttttt gatcatactt cttattagcg taatgactaa
     1141 taataccgca aatgcgacca acaattttcc ggtctctata tgctagaaac agtttaaaat
     1201 cgcaccacgc tgaagccgga tttttacttg gagttaaatt tgatatttcg tcttcaaaaa
     1261 tatacggagt aaattgtttt gaatttttat ataatttgac ggggaagttg atgaattgtc
     1321 taagttttct tccgccgtac acagggacta tttttatcat aatacattcc tctacttgat
     1381 aattttaaca taatcaaatt acatttgaag taaaacatat tatgaataat gaacgaaaat
     1441 aaaaaaacct cacgattgcg tgagttattt ttaaaaaaat ggtggatgct gagggaa
//
LOCUS       DAOSEX010000232         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_89059_length_1488_cov_2.945569, whole genome shotgun sequence.
ACCESSION   DAOSEX010000232 DAOSEX010000000
VERSION     DAOSEX010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89059_length_1488_cov_2.945569"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1222)
                     /locus_tag="PK612_04225"
     CDS             complement(89..1222)
                     /locus_tag="PK612_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOH95345.1"
                     /translation="MIYFDNASTTKIHPEVLRTYIEILEKYYGNPSGMHTFAREIARL
                     QDKSREEILRMFKFRDGKVIFTSGATEANNIALIGLYEAYKNRGNEIIISDYEHPSVR
                     DACEYLEKERGAIIHKVPIRFKGGFDFDVFKSLLNDKTVLVSVMAVNNEVGDVIDLGE
                     IRACLKKYPKVIFHTDVTQALGKLDFHPNAADAYSFSAHKIGGLKGSGALLLRKNLKP
                     KPLVYGGSQEEGIRPGTHNAPANIVLAKTVRIAEGNMDKFYDHSYRLALKLHNFLATH
                     KDLFIINSVGGSNPYIVNFSLKTIKASIVLNALESRDIYVGTTSSCSAKLHAMSKSVY
                     AISQDEERASNSIRVSFGMENTLEEVEIFCNTLTEIIKEYGRE"
     gene            complement(1219..>1488)
                     /locus_tag="PK612_04230"
     CDS             complement(1219..>1488)
                     /locus_tag="PK612_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95346.1"
                     /translation="ERPIDVSQVEVLTNELRALATRLEDVITTDVKYAHSAEEVIVSL
                     NAFRPQFSELHSKLRDEEQRFFNGLFKHTYENSIEQLKKYQNRKQ"
BASE COUNT          449 a          300 c          244 g          495 t
ORIGIN      
        1 ccggggtaaa atataccgtg ctgaccacgc gccatcacga cggtttttgc ctttcgtata
       61 aagttcacca aaatgaatca ttaacatttt actcacggcc atactccttt ataatttcgg
      121 ttaatgtgtt acaaaatatc tcaacttctt caagcgtatt ttccattcca aaacttactc
      181 ggattgagtt actagcgcgt tcttcatctt gcgaaatggc atatactgac ttactcattg
      241 catgtaattt agcgctacaa gagctagtcg tgccaacata aatatcgcga ctttccaagg
      301 cgttgagcac gattgaagct ttaattgtct ttagtgaaaa gttaacaata tacggattac
      361 ttccgccgac cgaattaata ataaataaat ctttatgagt agcaagaaaa ttatgtaatt
      421 taagtgctaa gcgataagaa tgatcataaa atttatccat attaccttca gcgatacgca
      481 ctgtttttgc taatacaata ttcgccggag cattatgtgt tcccggcctt atcccttctt
      541 cttgactgcc gccatacact agtggctttg gctttaagtt tttccgtaaa agtagtgcac
      601 cacttccttt taatccgccg attttatgag cggaaaatga ataagcatct gcagcgttgg
      661 gatgaaagtc aagtttgcct aaggcttgag tgacatcagt gtgaaatata actttaggat
      721 atttttttag acatgctcta atctccccta aatcaatgac atcaccaacc tcattattaa
      781 cagccataac ggagacaagc accgttttat catttaatag cgatttaaaa acgtcaaaat
      841 caaaaccacc tttaaagcga atcggcactt tatgaatgat tgccccacgt tctttctcta
      901 aatattcaca tgcgtcacgc acactagggt gttcgtaatc actaataatt atttcgttac
      961 cacgattttt ataagcttca tataaaccaa taagcgcaat attatttgct tcagtagcgc
     1021 cactagtgaa aataacttta ccatcacgaa acttaaacat tcttaaaatt tcttcgcgtg
     1081 atttatcttg taatcttgct atttcacgcg caaaagtgtg catcccactt ggattgccgt
     1141 aatatttttc aagaatctca atgtatgttc ttaaaacttc aggatgaatt tttgttgtac
     1201 tagcgttatc aaaataaatc attgtttacg gttttggtat ttctttaatt gttcgattga
     1261 attttcgtaa gtatgtttaa aaagcccatt aaagaagcgt tgttcttcat cacggagttt
     1321 tgaatgtaat tcactaaatt gtggacggaa agcgttaaga ctaacgataa cttcttccgc
     1381 tgaatgagcg tactttacat cagttgtaat cacatcttct aagcgtgtag caagcgcacg
     1441 taattcattt gttaaaactt cgacttggct aacatcaatt ggtcgttc
//
LOCUS       DAOSEX010000233         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_89708_length_1481_cov_2.805750, whole genome shotgun sequence.
ACCESSION   DAOSEX010000233 DAOSEX010000000
VERSION     DAOSEX010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89708_length_1481_cov_2.805750"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PK612_04235"
     CDS             <1..502
                     /locus_tag="PK612_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95347.1"
                     /translation="QFGYIVAIAIAIEAGVMFLLSLRKNNSFRMPAFSFFFIGIFLLI
                     RPLVIVLNLSPTWTMVLSVFRGIAWGYYIVFNVRFLARVVPLKHLTKALLTGSIATTS
                     SRIITSLIIGESLKHHSYQTVFFCSMFSVVFGMTISMIIAEVDRRKQKKVDMIVPFIE
                     KDQDLN"
     gene            complement(499..984)
                     /locus_tag="PK612_04240"
     CDS             complement(499..984)
                     /locus_tag="PK612_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HOH95348.1"
                     /translation="MEIRDVTNVLFIKNNKVLLGYKKRGFGKKKYNGFGGKLKSGETI
                     EMAAIREAKEEAGLTMKTYYKAAEIDFAESYPLRMHLYVCTEWSGEITESDEMVPRWF
                     GFNEVPVDEMWDDDKYWLHLALSGQKFRASFVFENDDDVTGTAENKVISYSIKLVDTL
                     D"
     gene            complement(1120..1323)
                     /locus_tag="PK612_04245"
     CDS             complement(1120..1323)
                     /locus_tag="PK612_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95349.1"
                     /translation="MVKEFGLPWIAHLLLQLFIGPIWGAVIRLVRGRVLWAVIYLLTG
                     GFFAIGWIYDLVMLIIHRDYKLA"
BASE COUNT          449 a          283 c          245 g          504 t
ORIGIN      
        1 acagttcgga tatattgtgg cgattgcaat agcaattgaa gcgggcgtga tgtttttact
       61 ttcgctacgt aaaaataata gtttccgaat gccagccttt tcgtttttct ttatcggaat
      121 attcttgctt attcggccgt tagtcattgt tcttaattta tcaccaacat ggacaatggt
      181 attgtcagtc tttcgcggga tagcctgggg ctattacatc gtttttaatg tgcgcttttt
      241 agctcgtgtt gtccccttaa aacacttaac aaaggcactt cttactggat caattgccac
      301 gacttcaagt cggattatta ctagtttaat cattggcgaa agtcttaagc atcattcgta
      361 tcaaacagtg tttttctgtt caatgttctc tgtagttttt gggatgacaa ttagtatgat
      421 tattgccgaa gttgatcggc gaaaacaaaa aaaggtcgat atgatcgtac cttttattga
      481 aaaagatcaa gatttaaatt aatctagagt atccactaat ttgatggagt aactgatgac
      541 tttgttctcg gcagtaccgg taacatcgtc atcattttca aaaacaaaac tggcgcggaa
      601 cttttgaccg ctaagtgcta gatgcaacca atatttatca tcatcccaca tttcatcaac
      661 tggcacttca ttaaaaccaa accatcttgg taccatttca tcagattcgg tgatttcacc
      721 gctccattca gtacatacat ataaatgcat ccgtaatgga tagctttccg caaaatcaat
      781 ttcagcggct ttataatatg ttttcattgt aagacccgct tcttctttag cttctctaat
      841 cgcagccatt tcaattgttt caccactctt taatttacca ccaaagccgt tatatttctt
      901 tttaccaaaa ccgcgttttt tataaccaag taaaacctta ttgtttttta taaataatac
      961 gtttgtaaca tctctaattt ccatatttgt tttctctttt caataatagt gtgttaagca
     1021 cattgcttaa tatacgctaa taagggggaa ataacaagaa aaaggaataa aaaaaacaca
     1081 ccagaggtgt gttcctttaa cttcactaat tttaattaat taggcaagtt tgtagtctct
     1141 gtggatgata agcatcacta agtcgtaaat ccagccaata gcgaaaaatc cgccagtgag
     1201 tagataaatg acggcccaaa ggacacgtcc gcgaactaag cgaataactg ctccccaaat
     1261 aggtccaatg aaaagttgga ggagtaagtg agcaatccat ggtaatccaa attctttaac
     1321 cattttgtac ctcctatggt ttatgatttt atgatatatc aattaataca aaatttcaat
     1381 ctaacaaatg taaattaata cttactaatg agcgctttca gttctttggg atcaattttt
     1441 attgaacggt catgggctaa taatccgttt ttctcttgat a
//
LOCUS       DAOSEX010000234         1476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_90121_length_1476_cov_3.976777, whole genome shotgun sequence.
ACCESSION   DAOSEX010000234 DAOSEX010000000
VERSION     DAOSEX010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90121_length_1476_cov_3.976777"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PK612_04250"
     CDS             complement(<1..476)
                     /locus_tag="PK612_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOH95350.1"
                     /translation="MNKKARDVLLYFASKYEGNYHAIINALRSKEPVDDALFNKYKED
                     WEKVDTITIIDEDYPALFKTVNNPPIVLFLKGNKELLFNLEKAIAVIGAREYSEYGKN
                     MTYEIVEGLVKEKFTIVSGLARGIDGFSHDACLRAGGNTIAVLGSGINYPYPKSNK"
     gene            complement(526..1152)
                     /locus_tag="PK612_04255"
     CDS             complement(526..1152)
                     /locus_tag="PK612_04255"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOH95351.1"
                     /translation="MTYFEFDNDVRKHGHLYLVGVDEAGRGPLAGPVFAAAVILPPFF
                     NDERIKDSKQLTATELLELSDLIKEKALDYQIVHVDAPVIDQVNIYQATILAMQKAVT
                     GLSHEFHFVLTDAMPLSNLEVGYEAIIQGDATSLSVAAASILAKVARDAYMNKLAKLY
                     PQYDFENNKGYPTRKHLQALDTYGPIKDVHRYSYKPVQDALNKQIKLF"
BASE COUNT          468 a          269 c          240 g          499 t
ORIGIN      
        1 tttttatttg atttaggata aggataatta attccgctgc ctaggaccgc tattgtgttg
       61 ccacctgccc gtaaacacgc atcatgacta aacccgtcta taccgcgcgc taagccacta
      121 acaatcgtaa atttttcttt aacgagtcct tcgacaattt cataagtcat gtttttaccg
      181 tattcactat actctcgagc accaatcaca gcgatagctt tttccaaatt aaaaagtaat
      241 tctttgttac cttttaaaaa taaaacaatt ggcggattat taactgtttt aaaaagcgct
      301 gggtagtctt catcaataat cgttatggta tcaacttttt cccagtcttc tttatattta
      361 ttaaataaag cgtcatcaac tggttctttg gagcggagtg cattaataat tgcgtggtaa
      421 ttgccttcat atttactggc aaagtaaagt aaaacatcgc gtgctttttt attcatttgt
      481 agttcctcat tatataaata aggaagaact taatttttta tcacattaaa ataatttaat
      541 ttgtttattg agcgcatctt gcactggttt ataagagtag cgatgcacat ctttaattgg
      601 gccataagta tcaagtgctt gtaaatgttt ccgtgttgga taacctttat tattttcaaa
      661 atcatattgc ggatagagtt tcgcgagttt attcatataa gcatcacgcg ctactttagc
      721 aagaatactt gcggcggcaa cactaagcga agtcgcatct ccttgaataa ttgcttcgta
      781 ccccacttct aaattagata gcggcatcgc atcagttaac acaaaatgaa attcatgact
      841 taagccagta actgcttttt gcattgctaa aattgttgct tgataaatat taacttgatc
      901 aataacggga gcgtcaacat gcacaatttg gtaatcaaga gccttctctt taattaagtc
      961 acttaattca agtaactccg ttgctgttaa ttgtttcgag tctttaattc gctcatcgtt
     1021 aaaaaagggc ggcaaaataa ccgcggcagc aaacacaggt cctgctaaag ggccgcgtcc
     1081 cgcttcatca actcccacta aataaaggtg tccgtgtttt cttacatcat tatcaaattc
     1141 aaaatatgtc atattatcac tccaaactta caagctagta attcaattac taacattatt
     1201 atattataga aactaactat ttctattttt aatatttcaa atatttgtga ttttgttcct
     1261 aaaatgccta agctagtatt gagatttcat ttttaagaat taaaatgaaa atatagatta
     1321 gtgaaaggag aagattatga aaaaacgaaa atttttacta atcccactac ttttaggatt
     1381 gctcttaact ggatgctttg gtggagacgg tcctgaagat attattgatg gcgacgatga
     1441 aagcagtcaa aaaactacta acgatgtagc ggaaga
//
LOCUS       DAOSEX010000235         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_90460_length_1473_cov_2.339210, whole genome shotgun sequence.
ACCESSION   DAOSEX010000235 DAOSEX010000000
VERSION     DAOSEX010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90460_length_1473_cov_2.339210"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PK612_04260"
     CDS             <1..475
                     /locus_tag="PK612_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOH95352.1"
                     /translation="NSFIPDAAHQTKAAAWYGTGTEVIFAAAGGAGGSVMKAAEEKQN
                     KWVIGVDVDQRAESRTVLTSAIKSLGSSVYQTLDKIYKDGVKGGTSETLDSTVGGVGL
                     PAKSDADFDRFQNFNKAQYDAVYELVKNGTITVYDDVEDPDYVALGGAKVVVTVE"
     gene            593..>1473
                     /locus_tag="PK612_04265"
     CDS             593..>1473
                     /locus_tag="PK612_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH95353.1"
                     /translation="MSYVIEMLNITKSFPGIKANDNVTLQLKHGEIHALLGENGAGKS
                     TLMSILFGLYQPDEGTIKVHGEVVNIKNPNDANDLKIGMVHQHFKLVEDYTVLENIIL
                     GREETKNGFLTTKNARKKILEIADHYGLHIDLDAKIEDITVGMQQRVEILKMLYRDAE
                     ILIFDEPTAVLTPQEIVELMDNMRDLIKEGKSIILITHKLNEIKMVADRCTVLRKGQY
                     IGTVDVKNTTVEEMAEMMVGRKVSFHIEREKSEPGEVILEVKDVNHFNDYLNKKDLTD
                     VSFNVRAGEIVVVAGIEG"
BASE COUNT          496 a          225 c          323 g          429 t
ORIGIN      
        1 taactccttt attcctgacg ctgctcacca aactaaagct gctgcgtggt acgggactgg
       61 gaccgaagtt atcttcgccg ctgctggtgg agctggtggt agtgtgatga aagctgccga
      121 agaaaaacaa aataaatggg ttattggtgt tgacgttgac caaagagcag aatcaagaac
      181 agtcttaaca tccgctatta agagtttagg ctcaagtgtg taccaaacct tagacaaaat
      241 ctataaagat ggcgttaaag gtggtacaag tgaaacttta gactcaaccg ttggcggtgt
      301 tggtttacca gcaaaatcgg atgctgattt tgatcgcttc caaaacttta acaaagcaca
      361 atatgatgcc gtttatgaac tcgttaaaaa tggtaccatt actgtttatg acgatgttga
      421 agatccagat tatgttgcct taggtggcgc aaaagttgtt gttactgttg aataataatt
      481 tttaacataa gctaactaaa ggccgcgcaa ttatgcgcgc ggccttttac tttattacca
      541 aattaaaaac ttcatgttat aattgattta aagttgtaaa ggagatttta ttatgtccta
      601 cgttattgaa atgttgaaca taacaaagag cttccctggc attaaagcta atgacaatgt
      661 tacgctgcaa ttaaagcacg gagaaattca tgctttatta ggagaaaacg gtgcagggaa
      721 aagtacattg atgtcgattc tttttgggct atatcaacct gatgaaggaa caattaaagt
      781 gcatggtgaa gttgtcaaca ttaaaaatcc aaatgacgct aatgatttaa agattggaat
      841 ggtccatcaa cattttaagt tagtggaaga ttatactgtg ttagaaaata ttattcttgg
      901 tcgagaagag acgaaaaacg gttttctaac aaccaaaaat gcgcgtaaaa agatcttaga
      961 aattgctgac cactatggtt tacacattga tttagacgca aagattgaag atattactgt
     1021 tggtatgcaa caacgtgtgg agattttaaa gatgctttat cgtgatgcgg aaattttaat
     1081 ttttgacgaa ccaacagcag tgcttacccc gcaagaaatt gtggagttaa tggataatat
     1141 gcgtgactta attaaagaag gaaaatcgat tattcttatc acacataaac ttaatgaaat
     1201 taaaatggtt gctgatcgct gtacagtact gcggaaagga caatacattg ggacagttga
     1261 tgtaaaaaac acaacagttg aagaaatggc agagatgatg gttggacgca aagttagttt
     1321 ccacattgaa cgcgagaaaa gtgaacctgg tgaagttatc cttgaagtaa aagatgttaa
     1381 tcactttaat gattatttaa ataaaaagga cttaacagat gtctccttta atgtgcgcgc
     1441 tggtgaaatt gtcgtcgtag cggggattga agg
//
LOCUS       DAOSEX010000236         1467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_90993_length_1467_cov_2.659348, whole genome shotgun sequence.
ACCESSION   DAOSEX010000236 DAOSEX010000000
VERSION     DAOSEX010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90993_length_1467_cov_2.659348"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PK612_04270"
     CDS             <1..236
                     /locus_tag="PK612_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95354.1"
                     /translation="PSETDTISFETSFSGAGYKYDSGNEKYANQFQFYLYKNTERYDW
                     FRIDKVTFDKTNGKFKCEVFSNVDNSTTTFTEV"
     gene            389..>1467
                     /locus_tag="PK612_04275"
     CDS             389..>1467
                     /locus_tag="PK612_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006303437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH95355.1"
                     /translation="MQEIKRDLYLNKLINAIGNRMIKVVTGIRRSGKSYLLNNLFKNY
                     LLESGVSADHIIILNMDERRNISYRDPDTLDEYIRSKIKDNEMYYVIIDEVQFVADFE
                     SVLNGFLNMHNLDVYVSGSNSKFLSNDIITEFRGRGQQIRVYPLSFSEFMSVTTLSKS
                     DALTEYLRYGGMPLIMSMKSDEAKARYLKDLFELTYLKDIIERNNVLRDDVLDELVNI
                     LASAVGSLTSVTKLTNTFKSKGVKELSKNTLIQYLDYLLDSFLIKKVSRYDVKGKKYI
                     QTLAKYYFSDIGLRNARLNFRQQEENHIIENVVYNELIRRGFNVDVGIVEIRNGNGIK
                     RLEVDFVCNEASRRYYIQVTTHLDT"
BASE COUNT          507 a          198 c          270 g          492 t
ORIGIN      
        1 atccgagtga gactgataca atttcctttg agacttcctt tagtggcgcg ggctataagt
       61 atgatagtgg caatgaaaaa tacgcaaatc aattccaatt ttatctctat aaaaataccg
      121 agcgttacga ttggtttaga attgataaag taacctttga taaaacaaac ggtaaattta
      181 aatgtgaagt attttcaaac gtcgataatt cgacaacaac atttaccgaa gtttaatagt
      241 aaaacattta cttataataa tattcgcact gaacaaaaac tacaaaaatg taagttttgt
      301 tcaatgactc ttgatattca caaacaaata ttttaaaata gtactgaaca aaaactacaa
      361 aagcgtaagt tttgttcagt gaggtgttat gcaagagata aaacgtgatt tatatttgaa
      421 taagttaatt aatgcaattg gcaaccgcat gattaaagta gtgactggta ttcggcgctc
      481 aggcaaatca tatttgttga acaatctttt caaaaattat ttattagaaa gtggtgttag
      541 tgcagaccac atcattattt taaatatgga tgaaaggcga aatatttctt atcgtgaccc
      601 cgatacttta gacgaataca ttcgcagcaa aattaaagat aatgaaatgt attatgtcat
      661 cattgatgaa gtgcagtttg ttgccgattt tgaatcagta cttaacggat ttcttaatat
      721 gcataactta gatgtttatg tttctggtag taattcaaaa tttctatcaa atgatataat
      781 cactgaattt aggggtagag gacaacaaat tcgcgtgtat ccactttctt ttagtgaatt
      841 tatgagtgtc accactttaa gtaaaagtga tgctttaact gaatatttac gctatggcgg
      901 gatgccgctt ataatgtcaa tgaaaagtga tgaagcaaag gcgcgttatc ttaaagattt
      961 atttgagctt acttatctca aagacattat cgagcggaat aatgtgctgc gtgatgatgt
     1021 tttggatgag cttgtaaata tattggcctc tgctgttggt tctttaacta gtgttactaa
     1081 attaacgaat acctttaaga gtaaaggtgt caaggaacta tcgaaaaata cgcttattca
     1141 gtatttagat tacctattag attcttttct tatcaagaaa gttagtcgtt atgatgttaa
     1201 aggtaaaaaa tatattcaaa ctttagcaaa gtattatttt agtgatattg gattacgtaa
     1261 tgccaggctt aatttccggc aacaagaaga aaatcacatt attgaaaatg ttgtctataa
     1321 cgaacttata agacgtggtt ttaatgttga tgttggtatc gttgaaatta gaaatggaaa
     1381 tggtattaag cgccttgaag ttgattttgt ttgtaacgag gcgagtagac gctattacat
     1441 tcaagtaacg acgcatcttg atactag
//
LOCUS       DAOSEX010000237         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_92559_length_1450_cov_23.542652, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000237 DAOSEX010000000
VERSION     DAOSEX010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92559_length_1450_cov_23.542652"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1333
                     /locus_tag="PK612_04280"
     CDS             122..1333
                     /locus_tag="PK612_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95356.1"
                     /translation="MRKHKGNRKHFTIRQRHYIEFAIGKGISIMEMANFLGFSRQSIY
                     REIQDNRYPKFNQKRALEYPRNLCLRLTKYPFVCNVCKFKQGCPHIKYYYRGDVANDN
                     ASDRLVVARRGTRLSKGELLNIEEIITPGIRKGQSIHHVYTANKDKINVSQRTIRNLI
                     NRNELSVRNIDLPLTVAQKPPKKEYAKVNTTRPLGTLFGRMHVDFKGLIGFGDHYAQS
                     DSVIGKRSDKKTILTIHFPTLSFMFGLLCEYKGWLPVNNHFYALREKLGPTLYEKAFP
                     VILVDRGNEFDRLFLLEDDNGEGTSKVFYADAYNSTQRAEIESNHRLIRRIIPKGPKG
                     RSFEFLTQNHLDLMFSHINGLIREKQGNRTGYELAKEYLGQDFLNAINIKYIAPDEVT
                     LKPYLFDKLQK"
BASE COUNT          487 a          248 c          266 g          449 t
ORIGIN      
        1 ataatagaag aaaaggttaa attgacaaga taaaaaactg aaagaatcat tttttgcaaa
       61 aaatggtttt tttagtttta ataaagttag gtgtgatttc cgtattgaaa ggaggttaag
      121 catgcgtaag cataaaggaa atcgaaaaca ctttactatt cgtcaacgtc attacattga
      181 gtttgctatt ggtaaaggaa tatcaataat ggaaatggct aactttcttg gattctctcg
      241 gcaatcaata tatcgggaaa ttcaagataa tcgttaccca aaatttaatc aaaaacgggc
      301 attagaatat ccgcgaaatc tgtgtctccg attaactaaa tatccgtttg tttgtaatgt
      361 gtgcaagttt aaacaaggct gtccgcatat taagtactat tacagaggtg atgtcgctaa
      421 tgataatgcc tctgatcgtt tagtcgttgc tagacgtggc actaggcttt ctaaaggtga
      481 attacttaat atcgaagaaa taattactcc tggcattaga aaagggcaaa gtattcatca
      541 cgtctacact gcaaacaagg ataaaattaa tgttagtcaa cggacaattc ggaatcttat
      601 taaccgcaat gaattaagtg ttcgtaatat tgatttacca ttaactgttg ctcaaaaacc
      661 gccaaagaaa gaatatgcga aagtaaacac gacacgacca ctaggaacct tatttggtcg
      721 aatgcatgtt gattttaaag gtcttattgg gtttggtgac cattacgctc aaagtgattc
      781 ggtgattggt aaaagaagtg ataaaaaaac tattctcacc attcatttcc caacattaag
      841 ctttatgttt ggacttctat gtgaatacaa aggatggtta cctgttaata atcatttcta
      901 cgctttaaga gaaaaacttg gaccaacact ttatgaaaaa gcgttccccg ttattcttgt
      961 tgatcgcggt aatgaatttg atcggctctt tcttctcgaa gacgataatg gtgaaggtac
     1021 ttccaaagta ttctatgcgg atgcttataa ttccacgcaa cgggctgaaa ttgaatcaaa
     1081 tcatcgcctt attcgtcgta tcatcccgaa aggtccaaaa ggtcgttctt ttgaattttt
     1141 aacgcaaaat cacctcgatt taatgttctc tcacatcaat ggtcttattc gggaaaaaca
     1201 aggtaatcgc actggttacg aactcgctaa agagtatctt ggtcaagact tcttaaacgc
     1261 aataaatatc aagtatattg cacctgatga ggtaacgtta aaaccttatt tattcgataa
     1321 attacaaaaa taaaaaaggg aattagaccg caactctaat accctttata cggaagtcgc
     1381 accttgttag taaagccctt ataacttgtc aatataagtt ttgcaaactt tgaggcggtt
     1441 cttttcgccg
//
LOCUS       DAOSEX010000238         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_92710_length_1449_cov_2.508608, whole genome shotgun sequence.
ACCESSION   DAOSEX010000238 DAOSEX010000000
VERSION     DAOSEX010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92710_length_1449_cov_2.508608"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK612_04285"
     CDS             complement(<1..387)
                     /locus_tag="PK612_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95357.1"
                     /translation="MSDTAEFVKVEVIDEENKEAMSRRKKFEIRKLVMSTYVTLVVIA
                     YLAMVFFLDKWHPHWVIFLSIPVVDSLVTAILEKKIALFSVDALIILAFVVVGAVTGK
                     WHPAWMILLLVPLWRYILNTIKRIKRI"
     gene            complement(406..846)
                     /locus_tag="PK612_04290"
     CDS             complement(406..846)
                     /locus_tag="PK612_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95358.1"
                     /translation="MQFNQDFDKIIGEDIYLSITEKNSGLIGGLPYYKYGIHLNDGTL
                     VGNILVRIGHNFNSYYSGNFVYQVSGAYRGQNLAVKALNAILVVAKYHKMEYVIFSAP
                     SSSKGYLRTFEKMGAKLLEIVTPPENYMYYYTDIEPLAIYRLDL"
     gene            complement(884..1369)
                     /locus_tag="PK612_04295"
     CDS             complement(884..1369)
                     /locus_tag="PK612_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95359.1"
                     /translation="MITFEKSKRYLFYYVGVLLVTITLFISWVVMIVKNPGSTSGDIV
                     LLSFLFLILFGGEALFLIIYLIRPKVVLSVDEEGAYLHLSKKKTKEVAFSNFSSVGNM
                     RRNLVIATKDSELYVVRFLKDHKKAEETLKTMFNTFITNHPERYFDMTESADKQKHEE
                     K"
BASE COUNT          490 a          289 c          213 g          457 t
ORIGIN      
        1 aatacgttta atgcgtttaa tcgtatttaa aatataacgc cataatggca caagtaatag
       61 gatcatccac gctggatgcc atttacccgt taccgcacca actacgacaa aggcgaggat
      121 aattaaagca tcaacggaaa ataaggcgat tttcttttct aaaatggctg tcactaatga
      181 atcaacgaca ggaattgata agaaaataac ccaatgtgga tgccatttat caaggaaaaa
      241 aaccattgcg aggtaagcaa taacgacaag cgtcacatat gtcgacatta ctaattttct
      301 aatttcaaat ttcttgcgtc gactcattgc ctctttattt tcttcatcaa tcacttcaac
      361 tttaacaaat tcagctgtat cactcataaa tgtgttcctc tctttttata aatctaggcg
      421 atatatcgcg aggggctcta tatctgtata ataatacata taattttcag gtggtgtgac
      481 aatttctaat aactttgcac ccattttctc aaaagtgcgt aaataaccct tgctcgaact
      541 tggcgcacta aagataacgt attccatctt gtggtacttt gcgacaacaa gaattgcgtt
      601 taatgcttta accgcaagat tttgtccgcg atacgctccg ctaacttgat aaacaaagtt
      661 cccactgtag taagaattaa aattatggcc gatgcgcact aagatgttcc cgactaaggt
      721 accgtcattt aaatgaatac catatttata ataaggtaaa ccaccaatta agccactatt
      781 tttctcagta atacttaaat agatgtcttc accaataatc ttgtcaaagt cttgattaaa
      841 ctgcatattt atcattcctc ctgtttcatt ataaacggaa tgattatttt tcttcgtgct
      901 tttgcttatc cgcactttca gtcatatcaa aataacgttc tggatgatta gtgataaaag
      961 tattaaacat tgttttaagc gtttcttcag cctttttatg atcttttaag aagcgcacaa
     1021 cataaagttc actatctttc gttgcaatca ctaaattacg gcgcatattc ccaacagagc
     1081 taaaattact aaacgctact tcttttgtct ttttcttact taaatgtaag taagcgcctt
     1141 cttcatcaac actgagtact actttaggac gaatgaggta aataattaaa aacaatgctt
     1201 cgccaccaaa taaaataagg aaaaggaatg atagtaaaac aatatcacca cttgtgctac
     1261 cagggttttt aacgatcatc acgacccacg aaataaacaa agtaatcgtt acaagtaaaa
     1321 ccccgacata ataaaataag tatcgtttag atttttcaaa agtaatcata aatcaagtgt
     1381 tttttagtaa ccggtacctt ttttaccagt aaagaaagca acaccattac tcgtgccaat
     1441 ccgattagc
//
LOCUS       DAOSEX010000239         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_93226_length_1444_cov_2.732181, whole genome shotgun sequence.
ACCESSION   DAOSEX010000239 DAOSEX010000000
VERSION     DAOSEX010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93226_length_1444_cov_2.732181"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PK612_04300"
     CDS             complement(<1..651)
                     /locus_tag="PK612_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95360.1"
                     /translation="MARKQPFNINDLASVQLGLASPDMIREWSNGEILKPETVNYRSQ
                     KPELDGLFCERIFGPSKDYECHCGKYRRIRHRGTVCEKCGVEVISKYVRRERMGHIEL
                     ASPCTHIWYLKGTPARIATLLDIPAKQLEEIVYFISHIVTDPGTSSVLSYKQFIDETT
                     GRQVFAEAIKEIQENIEEGSFEHAKGSELLERLEVTQTFDFFATSRYISKHTGAKFG"
     gene            complement(654..>1444)
                     /locus_tag="PK612_04305"
     CDS             complement(654..>1444)
                     /locus_tag="PK612_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOH95361.1"
                     /translation="RILPEADMPFMEDGTPIDIMLNPLGVPSRMNIGQVLEIHLGMAC
                     KKLGVKVASPVFDGMTNEEIFELMEEAKMHKDGKEILYDGRTGDRFDERISVGVMYLI
                     KLVHMVDDKLHARATGPYSLVTQQPLGGKAQNGGQRFGEMEVWALEAYGAANILQEIL
                     TIKSDDRVGRKRTYEAIVKGYDLPTPGIPEAFKVLVRELQGLAINVELRMKDGEILDL
                     DKLAKEQDADIRRSRRSTAVREEQEVFDPEETAVVYKNEERGDE"
BASE COUNT          378 a          339 c          269 g          458 t
ORIGIN      
        1 accaaactta gcgccagtat gtttactaat ataacgcgaa gtcgcaaaga agtcgaaagt
       61 ttgtgtcact tcaagtcgtt ctaaaagttc actacctttt gcgtgttcaa agctaccttc
      121 ttcaatattt tcttgaattt ctttaatcgc ttcagcgaac acttgacgcc ctgttgtttc
      181 atcaataaat tgtttgtaag aaaggacact gcttgtgcct ggatcagtca cgatatggct
      241 aataaagtag acgatttctt caagttgttt cgcaggaata tctaaaaggg tcgcaattct
      301 tgccggtgtc ccttttaagt accaaatatg cgtacacgga ctagcaagtt caatatgccc
      361 catccgttca cgacgcacat atttagaaat aacttcaaca ccacactttt cacaaactgt
      421 gccgcggtgg cgaattcgcc gatatttacc acaatgacat tcataatcct tactaggtcc
      481 gaagattcgt tcacagaaaa gaccatcaag ttcaggcttt tggctccggt agttgaccgt
      541 ttctggtttt aaaatttcac catttgacca ttcccgaatc atgtctgggg aagcaagtcc
      601 aagttggaca cttgctaaat cattaatatt aaatggttgt tttcttgcca taattattca
      661 tcccctcttt cttcattttt gtagaccact gctgtttctt ctgggtcgaa cacttcttgt
      721 tcttctctaa cagcagtact tctgcgggag cgacggatat ctgcatcttg ctctttagcg
      781 agtttatcta agtcaaggat ttcgccgtct ttcatccgta attcaacatt aatcgcgagt
      841 ccttgcagtt cacgcactaa tactttaaat gcttctggga ttcctggtgt tggtaagtcg
      901 tagcccttca cgattgcttc gtaagtgcgt ttacgaccaa cacggtcatc gctcttgatt
      961 gtgagaattt cttggagaat gttagcggca ccataagctt caagtgccca cacttccatt
     1021 tccccaaatc tttgtccacc gttttgtgct ttaccaccaa gtggttgttg tgtgacaaga
     1081 ctataaggtc cagtcgcacg cgcgtgcaac ttatcatcaa ccatgtggac aagtttaatt
     1141 aagtacatga caccaacact aatccgttca tcaaaacggt caccggtgcg gccgtcatat
     1201 agtatttctt taccgtcttt atgcatcttt gcttcttcca ttaattcaaa gatttcttca
     1261 ttagtcatcc cgtcaaatac tggagaagca actttaacac cgagtttctt acaagccata
     1321 cctaagtgaa tttcaagtac ttgaccgata ttcatccggc ttggaacacc aagggggtta
     1381 agcataatat caattggggt gccatcttcc ataaacggca tatcagcttc tgggagaatg
     1441 cgcg
//
LOCUS       DAOSEX010000240         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_94703_length_1429_cov_3.650655, whole genome shotgun sequence.
ACCESSION   DAOSEX010000240 DAOSEX010000000
VERSION     DAOSEX010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94703_length_1429_cov_3.650655"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK612_04310"
     CDS             complement(<1..423)
                     /locus_tag="PK612_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH95362.1"
                     /translation="MKLATKRLILRPFTLNDVDAVQKYSSKESVTRYMMWGPNKYEDT
                     KRFVSSVVSRNSETPRDVFDFIVTLKDGTVIGACGLYFDEYPKPPTLGWVFDDTYWNQ
                     GYGTETGRALLAFGFNTLKVKSILATCDNENIASARIME"
     gene            564..1226
                     /locus_tag="PK612_04315"
     CDS             564..1226
                     /locus_tag="PK612_04315"
                     /inference="COORDINATES: protein motif:HMM:NF040319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HOH95363.1"
                     /translation="MKQKKINNLIIDYILNNYDKETPIFTKDIYQKYSEISRSTIRSI
                     LSRLVKENNIVRIAEGIYSLPNYDCAIGTIAISNNEIINKKYIRNDGKIFGYRTGISF
                     ANKLGLTTQTAGVDSIISNCVANKKREVKLNKTRVIVNSPRVEVTANNYKLLQILDLL
                     NDYTSFSEINLEEGASTIFKYLEGVNLERKEVEKIVNSYPREAQVNFYKIRCQDAITS
                     KY"
     gene            1204..>1429
                     /locus_tag="PK612_04320"
     CDS             1204..>1429
                     /locus_tag="PK612_04320"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOH95364.1"
                     /translation="MLLHQNIKEFRKIVILTATHYGLQEYQIEKDYYISLFLKPLGNM
                     SKNIPIVFKGGTSLSKCYGVISRFSEDIDLS"
BASE COUNT          527 a          208 c          234 g          460 t
ORIGIN      
        1 ttccataatg cgggcactag cgatattttc attatcacag gtggcaagaa tgcttttaac
       61 tttaagtgta ttgaagccaa acgctaatag agctctgcct gtttccgtgc cataaccttg
      121 attccaatag gtatcatcaa atacccaccc tagcgttggt ggttttgggt attcatcaaa
      181 ataaagacca caagcgccaa taacggtgcc gtcttttaat gtaacaataa aatcaaaaac
      241 atcacgcgga gtttcactat ttcgactaac aactgaagat acaaatcgtt tggtgtcttc
      301 atatttattt ggtccccaca tcatatagcg agtaacactt tctttacttg aatatttttg
      361 cacagcatca acatcgttta gtgtaaaagg tcttaagatt agtcgtttag tcgctaattt
      421 catgatgtta cctccgtata atgttaatta tatcaatact ttattattta aaattgattt
      481 taaatgcaac agcaagtaaa cattaataag aaaaagtgtt gcaaagctta gtagagatga
      541 taaaatgtag acggagaata gcgatgaaac aaaaaaagat taataattta attatagatt
      601 acatattaaa taactatgat aaagagacac caatttttac taaagatata tatcaaaaat
      661 atagtgaaat tagtagaagt acaatccgct ctattttaag tcgtttagta aaggaaaata
      721 atatcgttag aattgcagaa ggtatttact ctttaccaaa ttatgattgt gctatcggta
      781 ccatagctat ttccaataat gaaattatta ataaaaaata tattcgaaac gatggaaaga
      841 tattcggtta tcgtacgggc ataagtttcg ctaacaaatt agggttaaca acgcaaactg
      901 caggagttga ttcaataatc tcaaattgtg tcgctaataa aaagcgtgaa gtgaaattaa
      961 ataaaacacg tgttattgtt aatagtccgc gtgtagaagt cacagcaaac aattataaat
     1021 tattacagat ccttgacctt ttaaatgatt acactagttt tagcgagata aatttagaag
     1081 aaggggcaag caccattttt aaatatttag aaggtgttaa tttagaaaga aaagaagtag
     1141 aaaaaatagt taatagctac ccgcgtgaag cacaagttaa tttttataaa attaggtgtc
     1201 aggatgctat tacatcaaaa tattaaagag tttagaaaaa ttgttattct aaccgctact
     1261 cattatgggc tccaagaata tcaaatagaa aaagattatt acatatcatt atttttaaaa
     1321 ccgcttggta atatgagtaa aaacatccct atagttttta aaggtggtac ctctttatct
     1381 aaatgttatg gagttattag tcgtttttct gaagatattg acctttctg
//
LOCUS       DAOSEX010000241         1427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_94994_length_1427_cov_2.180029, whole genome shotgun sequence.
ACCESSION   DAOSEX010000241 DAOSEX010000000
VERSION     DAOSEX010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94994_length_1427_cov_2.180029"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK612_04325"
     CDS             complement(<1..383)
                     /locus_tag="PK612_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95365.1"
                     /translation="MAGALTGFVGNTSGAVPPIGTDDHPFYGTFDGNGKTIKNLWVSS
                     DPLDWKEKPIGYEYIDVGASIGFFGNIAQLNDGAIIGEANNFYLENIEITNKVADSYA
                     GIVVGLVNGNIQAVGVKNAKMSLGTT"
     gene            complement(396..674)
                     /locus_tag="PK612_04330"
     CDS             complement(396..674)
                     /locus_tag="PK612_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95366.1"
                     /translation="MRDFKKVIYFSLITVTSFLALIISTMAFTTTAWFTTILHFNTHT
                     NASSISNYYAGGTGTETDPYLIATPRHVYNFSWLQNSGIYPTKTYFKL"
     gene            complement(683..1360)
                     /locus_tag="PK612_04335"
     CDS             complement(683..1360)
                     /locus_tag="PK612_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95367.1"
                     /translation="MLILFGILVSFTSFTVAWFSNIRNAQVHLSHVEVTAPDFFITEY
                     NVYPVTSMTKGTTTSSFTFTNTTATEMPKYDPNDIAYSQYQRVLVVQITYEYGGSAST
                     VFGAKTTTTTFTTGTTGTNPYDDNFTSNIYQFTPSPGVTLADSWTSATISTTNADVES
                     LVTLNPTPVKATNTMITTIYAGSTTLWFVIEYNESVMNYINNVRAAEIREVVYVDDVI
                     YYIGDGA"
BASE COUNT          449 a          260 c          265 g          453 t
ORIGIN      
        1 gtcgtgccaa gactcatttt agcgttttta acacctactg cttgaatatt gccattaact
       61 agcccaacaa caatgccagc ataactatcc gcaactttat ttgtgatttc aatattttct
      121 aagtagaagt tattagcttc accaataatc gcgccatcat ttaattgagc gatgtttcca
      181 aaaaagccga tagatgctcc aacatcaatg tattcataac caattggttt ttctttccag
      241 tctaaggggt cactagaaac ccaaagattc ttaattgttt taccgttgcc atcaaacgtc
      301 ccataaaacg gatggtcatc agtgccaatt ggtggtactg ccccactagt gttaccaaca
      361 aagccagtaa gagcacccgc catattaata tcgttttata atttaaaata tgttttagtt
      421 gggtaaatac cgctattttg cagccaagag aagttgtaga catgacgagg ggtagcaatt
      481 aaatatggat cagtttccgt gcccgtaccg ccagcgtagt aattactaat ggaactagcg
      541 tttgtatgcg tgttgaaatg taaaatagta gtgaaccatg ctgttgtggt aaatgccatt
      601 gtcgaaataa taagcgcgag aaaagaagta acagtgatga gtgagaaata tattactttt
      661 ttaaaatcac gcataattat ttttaggctc catctccaat ataataaatt acatcatcaa
      721 catatacgac ttcacgaatt tcggctgctc gtacattatt aatgtaattc ataacactct
      781 cgttatattc gataacaaac cataatgtcg tacttccagc ataaattgtt gttatcatcg
      841 tatttgtcgc tttaactggc gtggggttaa gtgttactaa tgactcaaca tcggcatttg
      901 ttgtcgatat agtcgcgctt gtccatgagt ctgcaagcgt gacaccagga cttggtgtaa
      961 attgatatat attgcttgta aagttatcat cataaggatt cgtgccagtt gtccctgtcg
     1021 taaaagtagt tgttgttgtt ttagcgccga aaacagtact cgcgctacct ccatattcat
     1081 aagtaatttg tacaacaagc actctttgat attgagaata agcaatgtca ttaggatcat
     1141 attttggcat ttcggttgca gttgtatttg taaaagtgaa agaagaagta gtcgttcctt
     1201 ttgtcatact tgtgacagga tagacattat attcagttat aaaaaaatca ggggcagtaa
     1261 cttcgacatg actaagatga acttgggcgt tacggatatt agaaaaccac gcaacagtaa
     1321 aactagtaaa tgaaacgaga ataccaaaaa ggatgagcaa attcatcgtg attaaaatta
     1381 caggttctac ggcgatcttt gttcaatgta cttattatcc tttttta
//
LOCUS       DAOSEX010000242         1421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_95621_length_1421_cov_2.738653, whole genome shotgun sequence.
ACCESSION   DAOSEX010000242 DAOSEX010000000
VERSION     DAOSEX010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95621_length_1421_cov_2.738653"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1093
                     /locus_tag="PK612_04340"
     CDS             89..1093
                     /locus_tag="PK612_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95368.1"
                     /translation="MKLKKFMLAIGVIGLLLTGCDDPNTSSSQTSGSETSEITEAERF
                     SAAGYSPLKGWPVESIRMHYTREGIDAAWVFTNVLTPTGFGQNTDLYVRSYEDELGID
                     VFKSFEVVRVTADATTFTNYVALYDGKTDYTVIVDEEHEFGIEAFSGKVRIGVSFVEE
                     NEAVPSATYFRFTVQEKEEFPGAPKVDRSAHRSEITFSNDFKVTTRSKEIVEWAFSDL
                     YKFKVEVGESSTNVGNIPNNSGVGYLTPQLRIYKDQVLTLNMEQGTMKQVIFHGVNYT
                     EDGVDVKSIDAFPVVEGAQQFKTANAVLYHFDADNTTSLSLVVTANARLLDVTVIYFL
                     "
     gene            1201..>1421
                     /locus_tag="PK612_04345"
     CDS             1201..>1421
                     /locus_tag="PK612_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH95369.1"
                     /translation="MQLLKPELNSLIQKEALDLFYKNGYQATLMRDLAAAVGMSVGNT
                     YRYYKSKHDLFTTLVQPVHEKLVELLEAT"
BASE COUNT          456 a          230 c          280 g          455 t
ORIGIN      
        1 aaagtgtaat aatgtatccg ctttcttact ctatcattta aaatattaaa tgaagtatga
       61 taatataact tgatagcgga ggttatgtat gaaactcaag aaatttatgt tagcaattgg
      121 agtaattggc ttactcttaa caggatgtga tgatcctaat acatcgagca gtcaaacttc
      181 tggtagtgaa acaagcgaaa ttacagaagc tgaaagattt agcgctgctg gttattcacc
      241 gcttaagggc tggccagttg aatcaataag aatgcactac actcgtgaag ggatagatgc
      301 agcgtgggta ttcacgaatg ttttaacacc aacaggattt ggtcaaaata ctgatcttta
      361 tgttcgcagt tatgaagatg aacttgggat tgatgttttt aaatcatttg aagtagtgcg
      421 cgtcactgct gatgccacaa cttttactaa ttatgttgcg ctctatgatg gtaaaaccga
      481 ttacacagtt atcgttgatg aagaacacga atttggtatt gaagctttca gcggtaaagt
      541 acggattggt gttagttttg tagaagaaaa tgaagctgtt ccgagcgcta cttatttccg
      601 ctttactgtt caagaaaaag aagaattccc tggcgcacca aaagttgacc gtagtgcgca
      661 tcgctctgaa atcactttct ctaatgattt taaagtaaca acaagatcaa aagaaattgt
      721 tgaatgggca tttagcgatc tttacaagtt taaagttgag gttggtgaat caagtacaaa
      781 tgttggaaat attccgaata atagtggtgt cggctactta accccacaac ttcgtattta
      841 taaagatcaa gtcttaactt taaatatgga acaaggaaca atgaaacaag ttattttcca
      901 tggtgttaat tatacagaag atggtgtaga tgttaagtca attgacgctt tcccagttgt
      961 cgaaggtgct caacaattta aaacggctaa cgctgtttta tatcactttg acgctgataa
     1021 cactactagt ctttcacttg tcgttacggc taatgcaaga ctactcgatg ttactgttat
     1081 ttatttctta taaaatatta attatttaca ttataaagcc catatttgat gggcttttta
     1141 tttttttctt gcaacgattt tagcattgct atacaatatt agtgaacagg tgtttacatt
     1201 atgcaacttt taaaaccaga acttaattca ttaatccaaa aagaggcgct tgacctcttt
     1261 tacaagaatg ggtatcaagc aactttaatg cgtgatttag cagcggcagt ggggatgagt
     1321 gttgggaaca cttatcgcta ctacaaaagc aaacatgact tatttactac gcttgtacaa
     1381 ccagtacatg aaaagcttgt tgagttactt gaagcaacgc a
//
LOCUS       DAOSEX010000243         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_95793_length_1419_cov_3.060850, whole genome shotgun sequence.
ACCESSION   DAOSEX010000243 DAOSEX010000000
VERSION     DAOSEX010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95793_length_1419_cov_3.060850"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PK612_04350"
     CDS             <1..502
                     /locus_tag="PK612_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95370.1"
                     /translation="IKFINYHYVLLFLVTIANLLFAFKYLDHIYNWYEYLLVGSLVLS
                     AIMLYLIFFIKVFEIKKHIIVVILQALSVVTSYLSFGLFAHISPFGKQNIVFGIFGYL
                     FALIGMLVLLNPRLRKWPIMDKVLQQDGTVLILRPRYFMLALYEWGFIAAQFLLMIVM
                     YAYLYV"
     gene            complement(552..743)
                     /gene="rpmB"
                     /locus_tag="PK612_04355"
     CDS             complement(552..743)
                     /gene="rpmB"
                     /locus_tag="PK612_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003865181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOH95371.1"
                     /translation="MARHCPVTGKGPLSGNKRSHSLRATRRRWGVNLQNYKVEVDGKV
                     QTVRMSARAYRSLNKSAKV"
     gene            910..>1419
                     /locus_tag="PK612_04360"
     CDS             910..>1419
                     /locus_tag="PK612_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOH95372.1"
                     /translation="MDLINKNIGEKIKSRRLRLGYSQLMLSELAHVSRDQISRIESGK
                     INFTLATLFKICRALRIKPHKLLKDAEQEEYIKSGDLRPFVKWAGGKTQILDELMQRI
                     PNEFNNYFEPFVGGGAFFLKLEHPRSVINDINSELMTAYSVIKDKEKFIALKKLLLQY
                     EEKHNEEFYY"
BASE COUNT          442 a          213 c          247 g          517 t
ORIGIN      
        1 tatcaaattt atcaattatc actatgtatt attgtttctt gtgacgatcg ctaatttatt
       61 atttgccttt aaatatttag atcatattta taattggtac gaatatttat tagtcggttc
      121 actcgtatta agtgcgataa tgctctatct tattttcttt attaaagtat ttgaaattaa
      181 aaaacatatt atcgttgtta tcttacaagc tttatctgtt gtcacaagtt atctttcatt
      241 tggtttattt gcgcacatta gtccatttgg taaacaaaat atcgtctttg gtatttttgg
      301 ttacttattt gcgcttattg ggatgttagt gttacttaat ccacgacttc gcaaatggcc
      361 gattatggat aaagttttac agcaagatgg aacggtactt attttgcgac cgcgctattt
      421 tatgctagcg ctttatgaat ggggttttat tgccgcacag tttttattaa tgattgtaat
      481 gtacgcttat ctttatgttt aagtagaaag aagaagataa taaaaaaaac tcactttgtt
      541 gtgagctttt tttatacttt ggcggacttg ttaagtgagc gatatgctct tgcagacatc
      601 cggacggttt ggactttgcc atcaacttca actttatagt tttgtaagtt tacgccccag
      661 cgccgtctag ttgcgcgaag agagtgactg cgtttattgc cacttaaggg tcctttgcct
      721 gttactggac aatgtctagc cataatttcc tccccattca ataaatgcta cattaagata
      781 acattaatgt tataaatttg caaatataaa aagttaaatt ttgaaaatta ttgcaaattt
      841 gcacatattg ttaataaata cctttgttat taatgtgtat tttagtccac attatgttat
      901 actctctcta tggatttaat taataagaat atcggtgaaa aaataaaaag tagaagattg
      961 cgtttaggct actcacaatt aatgttatcg gaacttgctc atgtatcccg tgaccaaatt
     1021 tcacggattg aaagcgggaa gataaatttt acattagcga cactatttaa aatttgtcgg
     1081 gcgcttagaa ttaaacctca taaattgctt aaagatgctg aacaagaaga atatattaaa
     1141 agtggggacc ttcgtccttt tgttaaatgg gcgggtggta aaacgcaaat attagacgaa
     1201 cttatgcaaa gaattcctaa tgagttcaat aattactttg aaccttttgt ggggggtggc
     1261 gcgttctttt taaaactcga acacccgcgt tccgtcatta acgatattaa cagtgaacta
     1321 atgactgctt atagtgttat taaagataaa gaaaaattta ttgcactgaa gaaactatta
     1381 ctacaatatg aagaaaaaca taatgaggaa ttttactat
//
LOCUS       DAOSEX010000244         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_95816_length_1419_cov_2.540323, whole genome shotgun sequence.
ACCESSION   DAOSEX010000244 DAOSEX010000000
VERSION     DAOSEX010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95816_length_1419_cov_2.540323"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PK612_04365"
     CDS             complement(<1..329)
                     /locus_tag="PK612_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH95373.1"
                     /translation="MFKSKFAIDPSLLHKDSVYTLNNGVKMPVIGFGTWQAKDGEEAY
                     ESVKAALRVGYRHIDTAEAYHNEESVGRAIRDSGIPREEIFVTTKLTNTHLTYEDAKQ
                     AINASLKR"
     gene            497..1048
                     /locus_tag="PK612_04370"
     CDS             497..1048
                     /locus_tag="PK612_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOH95374.1"
                     /translation="MSDPKVSRIIALLNELKRGNNDNFAEFYELTKYQIFYSLLSLTK
                     NHELAEDILAETYLKFLDNLKRIKANQNPLGFLLVTARNLAYDHFKKENRVSYIEDYL
                     LESDIGASTTDKYDDSDRLLARMQEILNETEYQVVVLYVLSELTHKEIAEYLDKPLGT
                     ITWTYSNAIKKLQKGLSDYARDE"
     gene            1041..>1419
                     /locus_tag="PK612_04375"
     CDS             1041..>1419
                     /locus_tag="PK612_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95375.1"
                     /translation="MNDDKIKKIIQENVTKPEIKLSAAQILAMHEERKRAKEKPKRIP
                     FFKRPTFKYSLGFLSAAFVIGITVYALRDTIVPPIISTSEGGETSTPSSGEVSTPSVP
                     VSEHNPDKIPHGKEGEFVFMTSSA"
BASE COUNT          473 a          277 c          255 g          414 t
ORIGIN      
        1 cgctttaaac ttgcattaat cgcttgcttt gcgtcttcat aagttaaatg agtatttgtt
       61 aatttagtgg taacaaatat ttcttcgcgc gggataccgc tatcacgaat agcgcgccca
      121 acactttctt cgttatgata agcttcggca gtgtcaatat ggcgataacc aacacgcagc
      181 gctgctttta cgctttcata agcttcttcg ccgtcttttg cttgccaggt gccaaaaccg
      241 atgacgggca tcttcactcc attatttagt gtataaacgc tatccttgtg taaaaggcta
      301 ggatcaattg caaattttga tttaaacatc taacccctcc tataaatatt ttaacaaata
      361 aattatactt ttttcagaga atgtgtgatt ttataagacg aagtcttaag tttgttataa
      421 tagtcgtgga aagaaaaaat ttaaatatta ccaataaaat gatgaactta atcgtattaa
      481 taatgaggaa gtgcaaatga gtgacccaaa agtcagtcgt attattgcat tactcaatga
      541 attaaaacgc gggaataatg ataacttcgc ggagttttac gaactcacaa agtatcaaat
      601 cttctattcg ctattatcgc tgacgaaaaa tcacgaactc gcggaagata ttctcgccga
      661 aacttatcta aagtttttag acaatctcaa acgcattaag gccaatcaaa atcctcttgg
      721 cttcttgctt gtgaccgcta gaaacttagc ctacgaccac ttcaaaaaag aaaatcgtgt
      781 ttcttatatt gaagattatc tccttgaaag cgatattggt gcgagcacaa ctgacaagta
      841 tgacgatagt gatcgcttac tcgccaggat gcaagaaatt ctaaacgaaa cagaatacca
      901 ggttgtcgtg ctttatgttt taagtgaatt aacacacaaa gaaattgccg aatacttaga
      961 taaaccatta ggcaccatta catggacata tagcaacgct attaaaaaat tacagaaagg
     1021 attaagtgat tatgcacggg atgaatgacg acaaaattaa aaaaatcatt caggaaaatg
     1081 tgacaaaacc tgagattaag ctttccgctg ctcagatttt agccatgcat gaagaacgta
     1141 aacgcgctaa agaaaagcca aaacgcattc ctttctttaa acgtccaaca tttaaatata
     1201 gcctaggttt cttaagtgca gcgtttgtaa tcgggattac agtctatgct ttaagagaca
     1261 ctattgtccc cccaattatt agcacaagcg aaggtggcga aacttcgacc ccaagcagtg
     1321 gtgaagtctc cactcctagt gtaccagtaa gtgaacataa tccggataaa atcccacatg
     1381 gtaaagaagg tgagtttgta tttatgactt cttctgccc
//
LOCUS       DAOSEX010000245         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_95909_length_1418_cov_2.994131, whole genome shotgun sequence.
ACCESSION   DAOSEX010000245 DAOSEX010000000
VERSION     DAOSEX010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95909_length_1418_cov_2.994131"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK612_04380"
     CDS             <1..202
                     /locus_tag="PK612_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95376.1"
                     /translation="NALRDVDNSVDTRNFFRGIQDILWNFGFKNIRFTARSLERSLDN
                     HYYSGRFGPDFNYSFYRYVDNC"
     gene            565..1260
                     /locus_tag="PK612_04385"
     CDS             565..1260
                     /locus_tag="PK612_04385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOH95377.1"
                     /translation="MDWVEKLKHKYNVDVLYDVFLRENDSLFFLIEESHDICDKPLIK
                     LFGTCEGVGLPYCVDTIDELLSHIDVKSQICDIRGANEYRDELHREINSDQTKFVLYL
                     PLDFKGTKRYLQLEIIKMFKRNISIVMLNQIDIKKVNVETLFFESYKDSLTNLSNFST
                     LLMHIDKTEDSHYFGFLDLDYFKVVNDTYGHKVGDEILAKVGWKLIEIADETVIMYRK
                     RADEFIIMTLGLD"
BASE COUNT          502 a          171 c          245 g          500 t
ORIGIN      
        1 aaatgctcta agggatgttg ataactcagt tgacacaaga aattttttta gaggaataca
       61 agacatttta tggaattttg gatttaaaaa catcaggttt acagctcgtt cgttggaaag
      121 gagtttagat aaccactatt attcggggcg ttttggtcct gattttaatt atagttttta
      181 tagatatgtt gataactgct gaacttttgt taaaaactgt gaggatttta tagatgcatt
      241 tgatggaata tctatttatg gtggaagtga tacttataga taattttagt taaaataaaa
      301 atttataacg gttatgcaaa tattaatata aaagcaatcg ataatgctta tacaatttta
      361 cctagttatg tatattggca caataataaa tgataccatt catcattaaa aaatatgact
      421 ccggccccat accaaaatgt ctatgttgac acatcaactt gaacaaatgt aaaaaatgtt
      481 caattattca tatatactta tggaattttg cataaaatcg tgcaataata aattattaat
      541 ttattaataa gaggtgattt catagtggat tgggttgaaa aactaaaaca taaatataat
      601 gtagatgttt tatatgatgt gtttttaagg gaaaatgatt cacttttttt ccttattgaa
      661 gaaagccatg acatttgtga taaaccttta attaaactgt ttggtacatg tgaaggtgtc
      721 ggtcttcctt attgcgttga tacgattgat gaattattaa gtcatattga tgttaaaagt
      781 caaatatgtg atattcgcgg cgctaatgag tatcgtgatg aattacaccg tgaaattaat
      841 agcgatcaaa ctaaatttgt gctctatctt ccacttgatt ttaaaggcac taaaagatat
      901 ttacaacttg aaattattaa gatgtttaaa cgtaatatat caattgtgat gcttaatcaa
      961 atcgatatta aaaaagttaa tgttgaaaca ctcttttttg agtcgtataa agatagttta
     1021 acgaatttat ctaatttcag cacattacta atgcatattg ataagacgga agatagtcat
     1081 tacttcggat tcttagattt agattacttt aaagttgtaa atgatacata tggtcataaa
     1141 gttggcgatg agattctcgc taaagtagga tggaaattaa ttgaaatcgc ggatgaaaca
     1201 gttatcatgt atcgcaaaag ggctgatgaa tttattatca tgacgctagg gcttgattga
     1261 actgaaacaa aaaaacttgt aaaacgtatt caaaaagcga ttcgctcagt tgaattaccg
     1321 gacattaaaa tagattgctc aattggtgtt gcggaatatc gtcataatac ctgacttttg
     1381 gacttaagcc aattttctag tgtaaaacta ctttttga
//
LOCUS       DAOSEX010000246         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_96259_length_1415_cov_2.455147, whole genome shotgun sequence.
ACCESSION   DAOSEX010000246 DAOSEX010000000
VERSION     DAOSEX010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96259_length_1415_cov_2.455147"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PK612_04390"
     CDS             complement(<1..547)
                     /locus_tag="PK612_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95378.1"
                     /translation="MKKFRTAFWLILAFLTLTSCGKKPSSESPSVTTSTDTTESETTT
                     SVGDSGSEVTTFTITWKNYDSQVLEVDKNVERGTMPKYDGATPTRPDDDTYTYSFNGW
                     DPEPVPVVADATYVAKYKTTLKEVVDPRDFVFADTGEGYTLCGYYGNASRVVIPETYN
                     DKPVISLEKWAFFNLDNLVSVT"
     gene            complement(692..>1415)
                     /locus_tag="PK612_04395"
     CDS             complement(692..>1415)
                     /locus_tag="PK612_04395"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOH95379.1"
                     /translation="LILKDLEVLYRRKDKVETKARVNKDKDSVFELEIINKLIPALEN
                     EVPARLVTTLTQAEKDYVKLNFGLLTIKPLIYVANISDTEYATYEDNIEYQKVKAIAA
                     KEGAELVAISAEIEAEIATLSSADQELYLAELGINESGLNKVINVAYKTLGLSSFFTT
                     GKDEVRAWTFKDGTSAQKCAGIIHSDFERGFIRAEVYTYNDFEKYGSELKIKEAGKVR
                     SEGKDYVMRDGDIVFFKFNVRK"
BASE COUNT          423 a          280 c          203 g          509 t
ORIGIN      
        1 gtgtcactga cactaaatta tcaagattga aaaatgccca tttttcgagc gatattacag
       61 gtttatcatt ataagtttca ggaatgacta ccctcgaagc atttccgtaa tatccgcata
      121 aagtgtagcc ttctcctgta tcagcaaaga cgaaatctcg cggatccaca acttctttca
      181 aagttgtttt atattttgct acatatgttg catcagcaac aacaggtaca ggctcaggat
      241 cccagccatt aaacgagtat gtatatgtat catcatcagg ccgtgttggt gtagcgccat
      301 catactttgg catcgtgccc cgctccacat ttttatcaac ttcaagtact tggctatcat
      361 aatttttcca tgtaattgta aaagttgtta cttcgctccc agagtcccct acacttgtgg
      421 tggtttcaga ttccgttgta tcagtgctcg ttgtaacact tggactttca ctacttggtt
      481 ttttaccaca tgatgtcaaa gttaaaaagg ctaaaataag ccaaaaggct gttctaaatt
      541 ttttcataaa aatcttcccc ctacgataac ttcattttac tttaaaaaga aagtaaagca
      601 aagacttttt ttactacttt attaaatttt gcaatgagta aaaataatta gttatttata
      661 atcaatatta tttaaaaatt caatttttta tttattttcg cacattaaat ttgaaaaaga
      721 cgatgtcacc atcacgcatt acatagtctt tgccttcact gcgtactttg ccagcctctt
      781 taatttttag ttcactgcca tatttttcaa agtcattata tgtataaact tcagcgcgaa
      841 taaagccgcg ttcaaagtca ctatgaataa tacctgcaca tttttgcgca ctagtgccat
      901 ctttaaatgt ccacgcccta acttcatctt taccggtcgt gaaaaaagaa gaaagaccta
      961 atgttttata tgcaacatta attactttat ttaaccctga ttcattaatg ccgagttctg
     1021 ctaaatataa ttcttgatca gcgcttgata aagtagcaat ttctgcttca atttcagcac
     1081 tgattgccac aagttctgcg ccttcttttg cggcaattgc ttttactttt tgatattcaa
     1141 tattatcttc ataagttgca tattctgtat cactaatatt agcgacataa ataagcggtt
     1201 taatcgttaa taaaccaaaa ttgagcttaa cataatcttt ttcagcttgt gttaatgtag
     1261 taactagtcg tgctggtact tcattttcta aagcggggat aagtttatta ataatctcca
     1321 gttcaaacac tgaatcttta tctttattta cccgcgcttt tgtttcaact ttatctttcc
     1381 tacgatatag aacttctaag tctttaagaa ttaat
//
LOCUS       DAOSEX010000247         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_96458_length_1413_cov_2.503682, whole genome shotgun sequence.
ACCESSION   DAOSEX010000247 DAOSEX010000000
VERSION     DAOSEX010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96458_length_1413_cov_2.503682"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PK612_04400"
     CDS             <1..212
                     /locus_tag="PK612_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95380.1"
                     /translation="IAKEAKNKVDIINFIKAQSEEKDSPLIKEIKALLKSDAAFKELL
                     TFLVKHIRSYEEALITLKEGEIKHE"
     gene            205..1200
                     /locus_tag="PK612_04405"
     CDS             205..1200
                     /locus_tag="PK612_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95381.1"
                     /translation="MNNFNIKEVQTITYSFNLLRIKNDLAYHEKEMGRTKEKTIGESD
                     DFFQKLGKDFSNTVNKNVFESNLKQYNLKQEHLQKIETFIKENDVEVISDLNELAIYI
                     NELFKDDVADIRAVFVAFNLIFTSPYYGTSIKEAVKKESFNEVAEALKLPKNFFVDYE
                     KDLLKKYHLISGKYPNDLLADALLVGTLAGIAAFFISPVLGVFTAGAVTTLTLGGMIS
                     AGVIIGILAGSARYGANLAGNRSEVRKAFKSMSAEETDFMLLQKLAVIEALAKYQGEP
                     TVDEAIQQLIDELINLKSDLDLALYGFRENKDNVNTRKKDSFHRFDKQLLKVLKI"
BASE COUNT          535 a          227 c          254 g          397 t
ORIGIN      
        1 atattgctaa agaagcaaaa aacaaagttg atatcattaa ctttattaaa gcgcagagtg
       61 aagaaaaaga tagtccgctt attaaagaaa taaaagcatt actaaaaagt gatgcagcgt
      121 ttaaagaatt attaactttc ttagtgaaac atataagaag ttatgaagaa gcacttatta
      181 ccttaaagga aggagaaatt aagcatgaat aattttaata ttaaagaagt gcaaaccatt
      241 acgtattcat ttaatctttt acggattaaa aatgatcttg cctatcacga aaaagaaatg
      301 ggacgcacca aagaaaaaac gattggtgaa agtgatgact ttttccaaaa gcttggcaaa
      361 gactttagta acaccgtaaa taaaaatgtc tttgaaagca atttaaaaca atataaccta
      421 aaacaagaac atctccaaaa aattgaaact ttcattaaag aaaacgatgt agaagttatt
      481 agtgatctta atgaactagc gatttatatt aatgaactat ttaaagatga tgtcgcagat
      541 attagagcag tattcgttgc ctttaactta atctttacaa gtccctatta cggaacatcg
      601 attaaagaag cagtgaaaaa ggaaagtttc aacgaggtgg ccgaagccct taaattaccg
      661 aaaaacttct ttgttgatta cgaaaaagat ttattaaaga aatatcacct catcagtggc
      721 aagtatccaa atgatttact cgctgatgcc ttattagtag gtactctagc tggtatagct
      781 gccttcttta tttccccagt tcttggcgta tttaccgctg gagcagtgac aaccctcact
      841 ttaggaggaa tgataagcgc cggagtaatt attggtattt tagcgggaag tgcaagatac
      901 ggggctaacc ttgcgggaaa tcgcagtgaa gtacgcaaag cctttaaaag catgagtgcc
      961 gaagagacag actttatgct cttacaaaaa ctcgcggtta ttgaagcact cgctaaatat
     1021 caaggggaac cgacagtaga tgaagctatt caacaactaa ttgatgaact aattaactta
     1081 aaaagtgatc ttgacttagc cttatatggc ttccgggaaa ataaagacaa tgtcaatacc
     1141 cggaaaaaag atagtttcca tcgctttgat aaacaattac ttaaagtatt aaaaatataa
     1201 agcactaaac aaaagaaaag gaacgaatca catcgctcct tttcattaac taattagtta
     1261 cttattatcc aaagagtttt ttcttggaga tgaagtagta tcctgctaag gaagatacac
     1321 ctaatgcact aataataaga gttgcagttg cactattgtc acttgaagga attcttccgg
     1381 gaacgcttga accaattgag gtatcaattc cat
//
LOCUS       DAOSEX010000248         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_96661_length_1411_cov_2.132743, whole genome shotgun sequence.
ACCESSION   DAOSEX010000248 DAOSEX010000000
VERSION     DAOSEX010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96661_length_1411_cov_2.132743"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PK612_04410"
     CDS             <1..281
                     /locus_tag="PK612_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOH95382.1"
                     /translation="VLVNNDFQKLSLMHAISRKLKRIVIQNIVLALSVVLLLIALNFV
                     VTLPMGLAVAIHEGSTILVILNGLRLLNFNEKHAIKKPKRSKFNKKIA"
     gene            406..>1411
                     /gene="hrcA"
                     /locus_tag="PK612_04415"
     CDS             406..>1411
                     /gene="hrcA"
                     /locus_tag="PK612_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOH95383.1"
                     /translation="MRYRRRELILKYTVELFIKTAQPVGSKSLIDNYNLNYSSATVRA
                     EMNALEGMGFLEKPHASAGRVPSKKGYEFYVANLREDEVNDNFRHHIATLVEEKTKSV
                     EQVINQTCEILSQMTNLVSVALGPEKKGETLQSIQVVALDKNMGTAIFVTNRGYVEHN
                     TFIVPPSTSIQEISKVANIFNERLVGTRISEVSAKMELLQPLLTDYIVEQEVLANAFT
                     DAFIRYARDRLSLYGKESLLEQPEFINDSEKLRKLISILDDPEKIRELITGNGDLSVN
                     IGGKDEDLTFISANVHVPGDQVGQIVLVGPKRMDYSKVLWALEYVTDELEKYFEIERE
                     I"
BASE COUNT          483 a          244 c          292 g          392 t
ORIGIN      
        1 gggtgcttgt caacaatgat ttccaaaagt tatcattaat gcacgctatt tcacgaaaac
       61 ttaaacgcat tgttattcaa aatattgtgc tagcgctcag tgtggtgcta ttattaattg
      121 cgcttaattt cgtggtgaca ttgccaatgg gattagcagt agcgatccat gaaggtagta
      181 ctattttagt gatattaaac ggtttacggc tcttaaattt taatgaaaaa catgcgatta
      241 aaaaaccaaa aagaagcaaa tttaacaaaa aaatagccta atttttggct ttttacgaag
      301 taattagtaa aaatttacga aatttagcac ttaaatgttg acagtgctat tttctttagt
      361 ataatatatt tagcaatcga aagttgcaag tgctaaggag gagttatgag atacagacga
      421 agagagttaa tcttaaagta tactgttgag ttatttatta agactgccca acctgttggt
      481 tcaaagtcat taattgataa ttataactta aactatagca gtgcaacggt tcgagcggaa
      541 atgaatgccc ttgaaggaat gggctttctt gaaaagccac acgctagtgc tggccgtgta
      601 ccaagtaaga aaggctatga attctatgtt gctaatttac gtgaagatga agtgaatgat
      661 aacttccgac atcacattgc gacgcttgtg gaagagaaga cgaaaagcgt tgaacaagta
      721 attaatcaaa catgtgaaat tctctcacaa atgacaaatc ttgtcagtgt cgcacttggc
      781 cccgagaaaa aaggtgaaac actacaaagt attcaagttg tcgcgttgga taaaaacatg
      841 gggacggcaa tatttgtcac taatcgcggc tatgtcgaac ataatacctt catcgtcccg
      901 ccttcaacaa gtattcaaga aattagcaaa gtcgctaata tcttcaatga acgcctagtc
      961 ggtacaagaa ttagtgaagt atcggcgaag atggaactct tacaaccact cttaaccgat
     1021 tacattgttg aacaagaagt cttagcaaat gccttcactg atgcatttat aaggtacgcc
     1081 cgtgaccggc ttagcttata cggtaaagaa agcttacttg agcaaccaga attcattaat
     1141 gatagtgaaa agctccgtaa actcatatca atccttgatg atccagagaa aatacgtgaa
     1201 cttatcacgg gaaacggtga cttaagtgtg aatatcgggg gcaaagatga agacttaacc
     1261 ttcataagcg ccaatgtcca tgtcccaggc gaccaggtcg gacaaattgt cctagttggt
     1321 ccaaaacgga tggactacag taaagtctta tgggcgttag aatacgtaac tgatgagctc
     1381 gaaaagtact tcgagataga aagagagatt a
//
LOCUS       DAOSEX010000249         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_96938_length_1408_cov_2.925351, whole genome shotgun sequence.
ACCESSION   DAOSEX010000249 DAOSEX010000000
VERSION     DAOSEX010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96938_length_1408_cov_2.925351"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PK612_04420"
     CDS             complement(<1..450)
                     /locus_tag="PK612_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOH95384.1"
                     /translation="MEKLTFIISDIEANQRLDKYLKKIMPNAPQSFIYKLIRKKDIRV
                     NNKKSDAAYILNEDDTVVIFLTEAQVEEFIVPYTFTKIRRPLNIIYEDENILVVNKDT
                     GLLVHASEFENELTLTNMVLTYLHDRGKFDPQKRGYIPSPVSRIDRDT"
     gene            513..1307
                     /locus_tag="PK612_04425"
     CDS             513..1307
                     /locus_tag="PK612_04425"
                     /inference="COORDINATES: protein motif:HMM:NF021075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HOH95385.1"
                     /translation="MFKKEGFAEPAGLFSLSHLGLVLFFGTLAVLFYALTKKWTKAQV
                     MRFFKIYSLVLIALEIFKIIWNIVNYGTSAESLNRFIPLYYCSIFLYAFVIVAFTKGK
                     IQKAAYTWIMYGGLASGLAFLIYPSSSLLFYPFYHFLCLHSILFHMNFILIGFLIFRH
                     ELYTPNVKDFWYFVSFSLVFMSLAVILNKVLGTNLMFFEKPLAILPFETLNEWSPLFF
                     QSLIFILQLFGPFIGTHFVFYLFSRFTPFKIYKRNEENLTTNNDNI"
BASE COUNT          417 a          242 c          234 g          515 t
ORIGIN      
        1 tgtatcacgg tcaatccgac ttacggggga gggaatatag ccacgctttt gtgggtcaaa
       61 tttaccacga tcatgaagat aagttaacac catattagtg agtgttaatt cattttcaaa
      121 ttcactagcg tgcactaaaa gtcctgtatc tttattaacc actaaaatat tttcatcttc
      181 ataaattatg ttaagtggtc gtcttatttt cgtaaaagta taaggcacaa taaattcttc
      241 aacttgcgct tctgttagaa aaatgacgac ggtgtcgtct tcatttaata tgtaagcagc
      301 atcactcttc ttattattta ctctaatatc ttttttacga attaatttat aaataaaact
      361 ctgcggggca ttaggcataa tcttttttaa atacttatca agacgttgat tagcctcaat
      421 gtcactaatg atgaatgtta gtttctccat actttaattt taccaaatat cagtgcgcat
      481 atggtaaaat tagacaatga aaacgagggc aaatgtttaa aaaagaaggc tttgctgaac
      541 ctgcgggact ctttagtctt agtcacctcg gattggtgct cttttttggg acgcttgcag
      601 tgctttttta tgcattaacg aaaaaatgga caaaagcgca agtgatgcgt ttctttaaga
      661 tatatagtct tgtattaata gcgttagaaa tatttaaaat aatttggaat atcgtaaatt
      721 atggcactag tgctgagagt ttaaatcggt ttatcccgct ttattattgc tccatttttc
      781 tctatgcttt tgtaattgtt gcatttacca aaggtaaaat tcaaaaggcg gcttatactt
      841 ggataatgta tggcggactt gcttcaggtt tagccttttt aatatatcct tctagttcgc
      901 ttttgtttta tcctttctac cacttcttat gtttgcattc tatcttattc catatgaatt
      961 ttatcttaat tggtttttta atcttccgtc atgaactcta cactccaaat gttaaagact
     1021 tttggtactt tgtgagcttt tcactcgtat ttatgtcact agcggtaatc ttaaataaag
     1081 tgttaggcac taatttaatg ttctttgaaa aacctttagc gatccttccg tttgaaacat
     1141 taaacgaatg gtcaccactc tttttccaat ccttaatctt tattctgcaa ttatttggac
     1201 cttttattgg cacacacttt gtcttttatc tctttagtcg tttcactcca tttaaaatat
     1261 ataaaaggaa tgaagaaaac ttaactacaa ataatgacaa catataatta aatttgctat
     1321 aataaccgca ctgctggaga aatacccaag aggctgaagg ggctggcttg gaaagctagt
     1381 aggtctgtca caggacgcga gggttcaa
//
LOCUS       DAOSEX010000250         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_98033_length_1398_cov_2.460908, whole genome shotgun sequence.
ACCESSION   DAOSEX010000250 DAOSEX010000000
VERSION     DAOSEX010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98033_length_1398_cov_2.460908"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PK612_04430"
     CDS             <1..315
                     /locus_tag="PK612_04430"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOH95386.1"
                     /translation="FKDNPLFAVALVTLLSEFVVLIILFITAKEYVIRHIFSLNNLKI
                     ILSAGVSLGLVMLIKHFLPAMAYLATVGLCIFIGGIIYIVMLLLLKEDLVYAQVKGVK
                     NG"
     gene            308..1228
                     /locus_tag="PK612_04435"
     CDS             308..1228
                     /locus_tag="PK612_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="HOH95387.1"
                     /translation="MANPNFKENKLKRLIAKSIQPLLRIFSKKTYVSWQYRYITGHKL
                     DWKNLKRYTEKLQYLRLYYYPRNASVIKAASRDGARERVKSKGLEKLLIPIVGIYDKV
                     EDIPFEALPKRFVIKATHACALNHICLDKDTLDVNALKKKMRRWLKMDYGKKTVEPHY
                     SKIKPRLIVEHYVGKSDSLPTEYKMHVFNGKARYLYVVSGRGKDIRYDNLYVDFTPFP
                     ESQFNHWSSSDEVPKKPKNYEELVKYAEMLASDLLFCRVDFFIVDDHIYFNEFTFTPA
                     KGTLIFDDDKADFIISEWFDISSAKKNKAA"
     gene            complement(1200..>1398)
                     /locus_tag="PK612_04440"
     CDS             complement(1200..>1398)
                     /locus_tag="PK612_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95388.1"
                     /translation="FTSIISFDLREVLGAETLKRVNGLMISYELLEHEFLKPGGVLDT
                     SLNEEFALMLYEVMLPYSSWR"
BASE COUNT          455 a          216 c          259 g          468 t
ORIGIN      
        1 tttaaagata atccgctttt tgcggttgcg cttgttacac tgctttccga atttgtagta
       61 ttaattattt tatttattac cgcgaaagaa tatgttattc gtcatatatt tagtcttaat
      121 aatctgaaga ttattttatc tgcaggtgtt agtcttggtt tagtaatgtt aataaaacac
      181 ttcttaccag caatggctta cttagcgact gtagggcttt gtatattcat cggcggaatc
      241 atttatatcg ttatgttact attattaaaa gaagacttag tgtacgctca agttaaagga
      301 gtgaaaaatg gctaatccta attttaaaga aaataagtta aaacgcttga ttgctaaaag
      361 tattcaaccg ctgttacgta tattttccaa gaaaacatat gtgtcgtggc agtatcgtta
      421 tattactggt cataaattag actggaaaaa tttaaagcgt tatactgaaa agcttcaata
      481 cttacggctc tactactatc cgcgtaacgc aagtgttatt aaagctgctt cgcgtgatgg
      541 ggcgcgggaa agagtaaaga gtaagggtct tgaaaaactt ttaattccaa ttgtggggat
      601 ttacgataaa gttgaagata tcccgtttga ggcattacca aagcgctttg tcattaaagc
      661 cacacacgct tgtgctttaa atcatatttg tttagataaa gacacacttg atgtaaacgc
      721 actaaagaaa aaaatgcgta gatggttaaa aatggattac ggtaaaaaaa cagtggaacc
      781 acattactca aaaataaagc cgcgcttaat agtggagcat tatgttggga aaagtgatag
      841 tttaccaact gaatataaaa tgcatgtctt taacggtaag gcccgctact tatatgtcgt
      901 tagtggacgc ggtaaagata tacgttatga taatttatat gtcgatttta caccttttcc
      961 tgaatcgcaa tttaatcact ggtcaagtag tgatgaagtg ccaaagaaac ctaaaaacta
     1021 tgaagaactt gtaaagtatg cggaaatgct cgctagtgat ttattattct gtcgcgttga
     1081 tttttttatt gttgatgatc atatttactt taatgaattt acttttacac ctgcaaaagg
     1141 aaccttaatt tttgatgatg ataaggctga ctttatcatt agtgaatggt ttgatatttc
     1201 tagcgccaag aagaataagg cagcataact tcatagagca ttaatgcgaa ctcttcatta
     1261 agtgaagtat caagtactcc acctggtttc agaaattcat gttctaataa ttcgtaactt
     1321 atcattagtc cgtttactct tttaagtgtt tccgcaccta atacttcgcg gagatcaaaa
     1381 ctaataatac tcgtaaat
//
LOCUS       DAOSEX010000251         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_98156_length_1397_cov_2.346498, whole genome shotgun sequence.
ACCESSION   DAOSEX010000251 DAOSEX010000000
VERSION     DAOSEX010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98156_length_1397_cov_2.346498"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..>1397)
                     /locus_tag="PK612_04445"
     CDS             complement(224..>1397)
                     /locus_tag="PK612_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95389.1"
                     /translation="RVTGWTVGEPEAFVASEHINPAIAGDTVLYFRPDASYFAEGYDH
                     LAAYFFVGGENQFVNLVEHQTGVYKVTAPTPAAPGGWEKVIFVSLKSATNAWEVDGLT
                     NVLKQTPDLIYDGTKDMYDTTLGAWKTLGSLVSQTPTTAGISSEKVRIWVNRGGHYAT
                     EGYQYLLKAGDVHYTATGYEKALVTDDVYFAYFDVPLSALTGKNVDLVIVDTNLYFQV
                     AVAGGPYAAQDNSKLWVVKQNMDETYSVTKGAVEGRIYNTFFAKVLEGYLTCSTSADN
                     GYEAFNLVDTNFLPRAGEHWDMQGDLGGEHGVKIADYTSEEDYATGVREATPATDAYA
                     KYVMMKSLYDAAHPSPEQFRINKVANNNIIYVLVIGMLFAIAAFGFVRFRKKANVA"
BASE COUNT          422 a          290 c          239 g          446 t
ORIGIN      
        1 gtcactactt ctagtcgttg aacttaatac ttcactaatc gttgtgcaac tacttaaaag
       61 catcatgagt actattaata aaagaaattt acggtttttc ataatgacta actcctataa
      121 taaaatcatt tttattataa actaaatacc cgcattttgc aaattttagt tgaaaaagga
      181 gcgattatcg tcgttcccta agtgttttgt taaattcgtt taattatgca acgtttgctt
      241 ttttacgaaa acgaacaaac ccgaatgcgg caattgcaaa taacatacca attactaaca
      301 cataaataat attattatta gcaactttgt taatacggaa ttgttctggt gatggatgtg
      361 cagcatcata taatgatttc atcattacgt attttgcata agcatcagtg gctggagttg
      421 cttcacgaac accagtggcg taatcttctt cacttgtgta gtcagcaatt ttaacaccat
      481 gttctccacc taaatcacct tgcatatccc agtgttcacc agcgcgtggt aagaagttgg
      541 tatccactaa attgaatgct tcataaccat tatcagcact agtggaacaa gttaaatacc
      601 cttctaatac tttcgcaaag aatgtattgt aaatgcgtcc ttcaacagcg ccctttgtaa
      661 ctgagtaagt ttcatccata ttttgtttaa caacccataa tttgctattg tcttgagcgg
      721 catatggtcc accagctact gccacttgga agtataggtt tgtatcaaca atcacaagat
      781 caacattttt accggtaagt gctgatagtg gtacatcaaa gtacgcaaaa taaacatcat
      841 cagtaactaa tgctttttcg taccctgtag cggtatagtg gacatcgcca gcttttagta
      901 aatattgata cccttctgtc gcataatgac caccacggtt cacccaaatc cgaacctttt
      961 cactactaat tccagcagtt gttggggttt gtgagactaa tgatcctaaa gtcttccaag
     1021 cacctaaagt agtgtcatac atatcttttg tgccgtcata aattaggtct ggggtttgtt
     1081 taagtacatt agttaatcca tccacttccc atgcatttgt agcggatttt aaactaacaa
     1141 aaatgacttt ttcccatccg cctggtgcag ctggtgttgg tgcggtaact ttataaacac
     1201 ctgtttgatg ttcaacaagg ttaacgaatt gattttctcc accaacaaag aaatatgctg
     1261 ctaagtggtc ataaccttca gcaaaatatg atgcatcagg gcggaagtat aacactgtat
     1321 cgccagcgat tgcaggatta atgtgttcag aagcaacgaa tgcttcgggt tcccccacgg
     1381 tccaaccagt cactctt
//
LOCUS       DAOSEX010000252         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_98985_length_1389_cov_2.977511, whole genome shotgun sequence.
ACCESSION   DAOSEX010000252 DAOSEX010000000
VERSION     DAOSEX010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98985_length_1389_cov_2.977511"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PK612_04450"
     CDS             <1..620
                     /locus_tag="PK612_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95390.1"
                     /translation="ITAGQTLMPVQLSILREYNNIEKTQALLYAYAESYNTKENADFT
                     IKQFYLTPELVGAFNEYYDGYRGLYTMSGTFLISENMNALTEFAVFDGEKYVALDLIS
                     SRINFAIQVSPDTFVDLSTAHTFATALQGTLTVNIVLYQTDNFLFNDILGMIFADSPI
                     DRTYQCRFTYRKVSYSNVNLQLVDYENANELASLPAVSLTFTRAK"
     gene            620..1114
                     /locus_tag="PK612_04455"
     CDS             620..1114
                     /locus_tag="PK612_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95391.1"
                     /translation="METRKIVIEIVSKEATTKSQKIIDKVQKRETTETIETIKEGTLV
                     SSLVVNQAFTQVKGLVKNAALYYMNKHFTLKENYLMQRNVDNALTTINKVASFGTSVA
                     GGFLLGNVPGAAVAAVGWVANEAFTAFKRQDQTMMGLNTTNIQTAFQRTRAGLSNGSR
                     GTQN"
     gene            1111..>1389
                     /locus_tag="PK612_04460"
     CDS             1111..>1389
                     /locus_tag="PK612_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95392.1"
                     /translation="MTKVWINNKLVENIATNFVMTNELNETLDSAIIKVQNSPKIKMK
                     PYDDVLIEIDSGAARISFTNEQYFEDGVVLETEIKESGADYETVTTVRY"
BASE COUNT          480 a          227 c          293 g          389 t
ORIGIN      
        1 ccataaccgc tggtcaaaca ttaatgcccg tgcaattaag tattttaaga gagtataata
       61 acattgagaa aacacaagcg ttattatatg cttatgccga aagttataac actaaagaga
      121 atgcagactt tactattaag cagttttatc taactcccga attagtaggg gcttttaacg
      181 aatattatga cggctatcgc ggactttata cgatgtcggg aacattttta attagcgaga
      241 atatgaatgc attaaccgaa tttgcggtct ttgatggcga aaaatatgtc gctctcgatt
      301 taattagtag tcgcattaac tttgcgattc aagtaagtcc agatactttc gtagatttat
      361 caacagctca tacattcgcc actgctttac aaggaacatt aaccgtaaat atcgtgcttt
      421 atcaaacgga taatttttta ttcaatgata tattaggaat gatattcgct gattcgccga
      481 ttgatagaac ttatcaatgt cgatttactt atcgtaaagt gagttattca aatgttaatt
      541 tacaattagt cgattatgaa aacgctaatg agttagcaag tttaccggcg gtcagtttaa
      601 cattcacgag ggctaaataa tggaaactcg taaaattgtt attgagattg tttcaaagga
      661 agcaacgacc aaatcgcaaa agataatcga taaggtgcaa aagcgagaaa ccaccgaaac
      721 gatagaaacg attaaagagg ggacattagt ttcaagctta gttgtgaacc aagcatttac
      781 acaagttaag gggttagtga aaaacgccgc attatactat atgaataaac actttacact
      841 gaaagaaaac tacctaatgc aacgaaatgt cgataacgcg ttaacaacga ttaataaggt
      901 ggcaagtttt gggacatcgg ttgctggtgg tttcttactt gggaatgttc caggagcagc
      961 agtggctgcg gtcggttggg tggcaaacga agcctttacc gcatttaagc gccaagacca
     1021 aacgatgatg ggactgaaca ccacaaatat tcaaacagcc tttcaacgaa caagagcggg
     1081 attgagtaat ggaagcaggg ggacacaaaa ttgacaaagg tatggattaa taataagtta
     1141 gttgaaaaca tcgcgactaa ttttgtaatg actaatgagt taaatgaaac attggacagt
     1201 gccattatta aggttcaaaa ctcgcctaaa atcaaaatga aaccttatga tgatgtcctt
     1261 attgaaattg atagcggtgc agcaaggatt agttttacta acgaacaata cttcgaggat
     1321 ggtgtcgttt tggaaaccga gattaaagaa tcgggtgcgg attatgaaac ggtgactacg
     1381 gtacgctat
//
LOCUS       DAOSEX010000253         1383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_99709_length_1383_cov_2.052711, whole genome shotgun sequence.
ACCESSION   DAOSEX010000253 DAOSEX010000000
VERSION     DAOSEX010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99709_length_1383_cov_2.052711"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PK612_04465"
     CDS             complement(<1..147)
                     /locus_tag="PK612_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOH95393.1"
                     /translation="MIKYLENAADFKEIISKGTVLVDFYADWCGPCRMLGPVLEKLSK
                     ERTDV"
     gene            complement(157..987)
                     /locus_tag="PK612_04470"
     CDS             complement(157..987)
                     /locus_tag="PK612_04470"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOH95394.1"
                     /translation="MQANRHLILNSFTLKIIAMSAVLIDHVALLFINPTLTIYILMRI
                     IGRIAFVLYAFFISEGVIHTKNTNKYLLRILYLYLIIQALIIGVLIYDPVIELRNIFA
                     TLGAGASLLIYFEKKEWKKVYYLLPFIIVSTINLLAYFTKDSWFIAFAGDNGFFGLAL
                     IIAFYVARKFSIYLLKKYPPSDNTAGEDAEKSAQHVYNVASCVSLLFVASIWYILSVY
                     NSEIPHVALQSFSLISIPLLLLYNGKLGHNSRSWRIIYYSFYPVHVVMLALLFFIIKL
                     "
     gene            complement(974..>1383)
                     /locus_tag="PK612_04475"
     CDS             complement(974..>1383)
                     /locus_tag="PK612_04475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOH95395.1"
                     /translation="GQAEENLNQQIINLILSKHPVSSFFWIVLLGPLVEELTYRVGLF
                     NAISSRNRIAAYVVSALVFGFLHFNIPLNADGAIDQAKLIEEFINIPSYIVSGLVFGY
                     IYEKEGFAGSSVAHITNNLISYILSIISIYASK"
BASE COUNT          500 a          247 c          240 g          396 t
ORIGIN      
        1 gacatcagtg cgttccttac ttaatttttc aaggactgga ccaagcattc ggcacggacc
       61 acaccaatca gcgtaaaagt cgactaagac tgttccttta ctaataattt ctttaaaatc
      121 tgctgcattt tccaagtatt taatcataag tgcctcctag agtttaataa tgaagaatag
      181 tagtgcaagc attactacat gaacgggata gaaagaataa tagataattc gccaactacg
      241 actattatgg cctaatttac cgttatagag taacaagagc ggaatactta taagtgagaa
      301 actttgcaat gcaacatgcg gtatttcgct attgtacacg cttaaaatat accaaatgga
      361 cgcaacgaat aaaagagaaa cgcaacttgc gacattatac acatgctgtg cgcttttttc
      421 agcatcctct ccagcggtat tatcactagg cggatacttc ttcagtaaat agatactgaa
      481 tttacgcgcg acatagaaag cgatgattaa ggctaaacca aagaagccgt tatccccggc
      541 aaaagcaata aaccacgaat ctttagtaaa ataggctaat aaattaattg tcgaaacaat
      601 aataaacggt aataagtaat acactttctt ccattctttt ttctcgaaat agatgagtaa
      661 acttgcacca gcgccaagcg tagcgaaaat atttcttagt tcgataactg ggtcgtaaat
      721 taaaacgcca atgataagtg cttggattat taaatataaa tataaaatgc gcaataagta
      781 tttattggta tttttagtat gtatcactcc ttcacttata aaaaaagcgt aaagtacaaa
      841 agcaatgcgc ccaataatgc gcattaggat ataaattgtt aatgtgggat taataaaaag
      901 aagcgcaaca tggtcaatta aaactgcact cattgcgata attttaagcg tgaatgaatt
      961 taatattaaa tgtctatttg cttgcataaa tactaataat tgaaagaata tacgatatta
     1021 aattgttagt gatatgggca acacttgacc cagcaaaccc ttctttttca taaatatagc
     1081 caaagacgag tccgctaaca atgtaactag gaatattaat aaattcttca attagtttag
     1141 cttgatcaat agcgccatca gcgttaagtg gaatattaaa gtgtaagaaa ccaaacacaa
     1201 gtgcgctaac gacgtaggct gcgatacgat tccgtgacga tatcgcatta aataacccaa
     1261 cgcgatacgt taactcttcc actagtgggc caagtaagac aatccagaaa aaggaactga
     1321 caggatgctt cgataagatt aaattaatta tttgttgatt taagttttct tcggcttgac
     1381 cga
//
LOCUS       DAOSEX010000254         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_99906_length_1381_cov_2.505279, whole genome shotgun sequence.
ACCESSION   DAOSEX010000254 DAOSEX010000000
VERSION     DAOSEX010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99906_length_1381_cov_2.505279"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1381)
                     /locus_tag="PK612_04480"
     CDS             complement(<1..>1381)
                     /locus_tag="PK612_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOH95396.1"
                     /translation="FAGAHFLEEYAEAKSAKEITNLLKITPTTARLLLQDGTQQEVSV
                     ESLKVGDKVLVLNGDQIPTDGTIVSGSAAINEAMITGESMPKEKHEGDEVFGGSINGN
                     STFTMVVTKNSDETVLAKILRVVSETQTNLTKTATFIKKFEPIYVTIVMLIAPLFYLF
                     GYYVLGWNEVISANGMTLALKRTIILFIGASPCALAVSDIPASLSALSLLARRGVLFK
                     GASSLALFADVEAVAFDKTGTLTEGKPSVTDVNIPPGVSAEEAEEGKVFLVSMEKKSN
                     HPLATAIVSHFTPIKTVDLEVEHKIGSGLRAMYDGNEYLVGKPALFNLPERCANRAKE
                     LAVQGKTVVFIAKNGKPILLVALLDMIKCCAVDGLEYFRKQEINTVMITGDNELTAKA
                     IGKELGITRIYANVLPEQKLDIVKELKKEYGGVAMVGDGINDAPALVEATIGIAMGDG
                     TDVAIDVADG"
BASE COUNT          393 a          335 c          210 g          443 t
ORIGIN      
        1 cccatcagca acatcaatcg ccacatcagt gccatccccc attgcaatcc cgattgtggc
       61 ttcaacaagc gcgggcgcat cattaatccc gtctcccacc atcgctactc cgccgtattc
      121 cttttttagt tctttaacaa tatcaagctt ttgttcaggt aatacattcg cgtatattcg
      181 cgtaatgcct aactctttac caatagcttt tgccgttaat tcattatctc cagtaatcat
      241 tactgtatta atttcttgtt ttctaaagta ttcaagccca tcaacggcac aacatttaat
      301 catatctaaa agcgctacaa gtaagattgg tttcccgttt tttgcaataa agacgactgt
      361 tttaccttga actgccaact ctttagcgcg attcgcgcag cgctctggta aattaaataa
      421 tgctggttta cctacaagat actcgttacc gtcatacatt gcgcgtaatc cactgccaat
      481 tttatgttca acttctaaat caactgtttt tataggagta aaatgactaa caatcgctgt
      541 cgctaatggg tgattacttt tcttttccat actgacaagg aaaacttttc cttcttctgc
      601 ttcttctgca cttactcctg gcgggatatt aacatcagta acactcggtt tcccttccgt
      661 taaagtacca gttttatcaa aagcaactgc ttctacatcc gcaaatagag ctaaggaaga
      721 agcaccttta aaaagcacac cgcgtcttgc taaaagactt aaagcactta gacttgccgg
      781 gatatcacta acagctaggg cacacggcga agcaccgata aaaagaataa ttgtgcgttt
      841 aagcgcaagc gtcatcccgt tagcgctaat aacttcattc caccctaaaa cataatagcc
      901 aaataaataa aatagtggag caattaacat cacaatagtt acataaattg gttcaaattt
      961 cttaataaat gtcgcagttt tagttaaatt agtttgcgtt tcactcacaa cccttaagat
     1021 tttcgctaat accgtttcat cactattttt ggtcactacc atcgtaaaag tactgttgcc
     1081 attaatacta ccaccaaaga cttcatcacc ttcatgcttc tcttttggca tactttcacc
     1141 cgtaatcatc gcttcattaa ttgcggcact accactaact atcgtgccat ctgtcgggat
     1201 ttgatcacca tttaacacaa gtactttgtc accaactttt agtgactcaa cacttacttc
     1261 ttgttgtgtt ccgtcttgaa gtaaaagcct agcggttgtc ggtgttatct ttaaaagatt
     1321 cgtaatttct ttggcacttt ttgcttcagc atattcttca agaaaatgcg cgcccgcgaa
     1381 t
//
LOCUS       DAOSEX010000255         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_99989_length_1380_cov_3.845283, whole genome shotgun sequence.
ACCESSION   DAOSEX010000255 DAOSEX010000000
VERSION     DAOSEX010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99989_length_1380_cov_3.845283"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PK612_04485"
     CDS             complement(<1..1032)
                     /locus_tag="PK612_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH95397.1"
                     /translation="MRTIIKRKDKKALLKYVKKMHPYDIIEALNALTLDEQELFYSLL
                     SEEEIGRIVAFMEPEKAAEVIDDFDLTKQKNIIDTLELDDAADIIVYLDNETKLIEGL
                     EKELHITQVLKYDEEKVGAHMSDAYISLSLGTDIKAATKNVIQKAGEIDVINHIFIVD
                     SGNKYKGTVELRDLVRTKAPALIDDITEFTPFVFASDDIEESVHKMKNYGLHEMAVVS
                     EELELIGIITIDDAIEIYHEESVDDFEKLSALPDTKTKGVIKSAAKRLPWLFLLLVLS
                     MPIAIATSRFEEVITSVAVLALFQPLILDAGGDVASQTLAVTLRLLSKNPKKALRNGG
                     TEILTGIING"
     regulatory      complement(1107..1272)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
BASE COUNT          422 a          259 c          224 g          475 t
ORIGIN      
        1 cccgttaata attcctgtta gtatttcggt gccaccattt ctaagcgctt tttttggatt
       61 tttactaagt aatcttaaag ttaccgcaag ggtttgactc gcaacatcac ccccagcgtc
      121 aagaattaat ggttggaaaa gggcgagtac cgctactgaa gtaattactt cttcaaagcg
      181 acttgtagca attgcaattg gcattgatag cactagtagt aagaagagcc acggtaatct
      241 tttagcagcg gacttaataa ctccttttgt ttttgtatct ggtagtgccg atagcttttc
      301 aaaatcatca acggattctt catgatatat ttcgatcgca tcatcaatcg taataatacc
      361 gataagctct aattcttcgc tgacaactgc catttcatgg agaccataat tcttcatttt
      421 atggactgat tcttcaatgt catcactggc gaaaacaaac ggagtaaatt cagtgatatc
      481 atcaataagt gctggtgctt tagtgcggac taaatctctt aattcgaccg tccctttata
      541 tttattgcca ctatcaacaa taaagatgtg attaataaca tcaatttccc cagctttttg
      601 aataacattt ttagttgccg ctttaatatc tgttcctaaa gaaaggctaa tatatgcatc
      661 agacatatgg gcgccaactt tttcttcgtc atactttaat acttgcgtaa tatgaagttc
      721 tttttctaaa ccttcaatta acttagtttc attatcgaga tagacaataa tgtcagcagc
      781 gtcatcaagt tcaagcgtat caataatatt tttttgtttt gttaaatcaa aatcatcaat
      841 aacttctgca gctttttctg gttccataaa agcaacaatt ctgccaattt cttcttcgct
      901 tagcagtgag taaaatagct cttgttcatc aagagtaagt gcatttagtg cttcaataat
      961 gtcataagga tgcatttttt tgacatattt aagcaatgct tttttgtctt ttcgtttaat
     1021 aattgtgcgc atgttaacct ccctcctttg cttggaattc aaataaaaag ctcccacatt
     1081 aagtgagagc taactaaaca gtacgagtat cagcgtattc gtagagtttt agcactatgc
     1141 aacgtaaaca tgaagttagc tactttgtgg aaagccttaa gtcgacaatc catgttctac
     1201 ccattggtgt ctctggacat ttttgggcag cagcctatgt ttgcatagga gcctcaccta
     1261 acggatttat ttaatttcaa agccataata gcatatatat tatatttgtc aatctactat
     1321 tttaaaaata atagaagaaa aggttaaatt gacaagataa aaaactgaaa gaatcatttt
//
LOCUS       DAOSEX010000256         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_100010_length_1380_cov_2.804528, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000256 DAOSEX010000000
VERSION     DAOSEX010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100010_length_1380_cov_2.804528"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..676)
                     /locus_tag="PK612_04490"
     CDS             complement(221..676)
                     /locus_tag="PK612_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95398.1"
                     /translation="MSKKRRIKELEAQVAALEAKVEGKEGQQTPKKAAKNSVNVYFDS
                     NFVNSLVSNQMKYGRRSRRDDLNVDPADKKAVRRARARRFFRFLWRLIVVLVIAALVA
                     AVVAAILMVIVWALVNFNIMTPTSNPFVGMVWEWIKWFFALFGMEVPIF"
     gene            complement(695..>1380)
                     /locus_tag="PK612_04495"
     CDS             complement(695..>1380)
                     /locus_tag="PK612_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95399.1"
                     /translation="KKELQKQKEHYEAIIAEQEKERSMRISRTEYDEVLAEYGRVKAL
                     LDEKDIEKLEEKPVEPKPAPQQVPQYEEPYYGSLHAKVDRLTDKVHELQLEILRKELA
                     NQKATPAPQEEQPETEVNIYFDSNLAKRMSDNKNAIDVGKAVKGKKRKKKSVGRTIFW
                     IIFILLLLAIVVFMGAWTLEHFNITPLAGPPWLADRFNAAVLWLHNAVQSVFDLFKGL
                     FGKVRVLGR"
BASE COUNT          369 a          288 c          248 g          475 t
ORIGIN      
        1 aatcgggcta aaggtacgca atactttttg aaatatacgg tcgcctaatt ttgacatcgc
       61 atgcactcct ttccgctata gtgtttttgt aatgatattt tttaattgaa cttatgggtt
      121 taatttactt ataacaatat ttgtgattct tctcattttt attatagcaa aaaagggact
      181 tggggtccct ttgatttgct tttatctaaa tggtgtcaat ctagaagatt ggtacttcca
      241 tcccgaagag ggcaaagaac cacttaatcc actcccagac catgccgaca aatggatttg
      301 atgttggggt cataatatta aagttaacaa gtgcccagac gataaccatt aatatcgctg
      361 cgacgaccgc tgcgactaaa gcggcaataa caagcactac aattaatctc cataagaaac
      421 ggaagaaacg acgagcgcgt gctcttctga cggccttttt atcagcggga tcaacattta
      481 aatcatcacg acgtgaacgc cgtccatact tcatttgatt tgatactagg ctattaacaa
      541 agttactatc aaaatagaca tttacggagt ttttagcggc tttctttggc gtttgttggc
      601 cttctttgcc ttcaaccttt gcttcgagcg ctgctacttg agcttctaat tctttaattc
      661 tacgtttttt actcataact caaatctctc ctttctatcg tcctaatacc cggactttac
      721 caaatagtcc tttaaataaa tcaaagacac tttggacagc gttgtggagc cataataccg
      781 cagcgttgaa acgatcagcg agccatggtg gtcctgcaag aggggtaata ttgaaatgct
      841 ctaatgtcca tgcacccatg aacacgacta ttgctaagag caagagaata aagataatcc
      901 agaatattgt ccgtccaaca ctcttctttt tacgtttttt acctttaact gctttgccaa
      961 catcaatcgc gtttttattg tccgacatcc gtttagcaag gttactatca aaataaatat
     1021 taacttctgt ttcgggttgt tcttcttgcg gtgccggagt agctttttga ttagctaatt
     1081 ctttacgtaa gatttctagt tgcaattcat gaactttatc cgttaagcga tcaactttag
     1141 cgtgaagtga accgtaatat ggttcttcat attgcggcac ttgttgcggt gcaggctttg
     1201 gttcaactgg tttttcttca agcttctcaa tgtctttttc atcaagtaat gctttaacac
     1261 gaccatattc cgctaaaact tcatcgtatt cagtacggct aatacgcatg gaccgttctt
     1321 tttcttgttc agcaataatt gcttcgtagt gttctttttg cttttgtaat tcttttttgc
//
LOCUS       DAOSEX010000257         1372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_100919_length_1372_cov_2.399393, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000257 DAOSEX010000000
VERSION     DAOSEX010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100919_length_1372_cov_2.399393"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="PK612_04500"
     CDS             complement(<1..1364)
                     /locus_tag="PK612_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH95400.1"
                     /translation="MKRRKLLLIPLIALLLGGCGDSSSSAVVTSEAPVTSVSEEVTPT
                     GITISGPGSLAVDESAYFNVTVKPEGAPQSVTWSTSDATKATVTESGEVTGVSEGNVF
                     VIATSTVKAEVTAQVPLVITPKVIVAPDPTEIFITSVDDLTTVSVDKTLKLNATVLPV
                     DASQEVNWSSSDETKATVDATGLVTGVALGTVNITATSAVKESVKKVFTLEVVEDAIT
                     QNWEEMAYSSHADFVNKNTEDGTPFKVRGVVTYAAPVTAEGTVEHFIQNGTEGYYIYK
                     QDNDLFPVTVGNSYTVGGYFKNYFGTFELVDVEYFVPHDPALTYEVVDISDKDVLSLD
                     IMAEHQASFVEIKAAVVSVLPTNYTKAYDVKVNVGDKEFTLRVDPALMSADEFNAITT
                     LFQGTAPGSAIDVVGVMSAFGYGTPANQMKIVRSTDITAAGMTDKQATEVAAASLEIV
                     ESIPL"
BASE COUNT          396 a          309 c          266 g          401 t
ORIGIN      
        1 agcgggatgc tctcaacgat ttctagactt gcggccgcga cttctgtggc ttgcttatca
       61 gtcatccctg ctgccgtaat atcagtgctt cgtacaatct tcatttggtt agcgggagtg
      121 ccatagccaa acgcactcat aacaccaaca acatcaatcg ctgaaccagg agcggtgcct
      181 tggaacaaag tcgtaatcgc attaaattcg tcagcactca ttaacgccgg atcaacacga
      241 agtgtaaatt ctttatcacc aacattaact ttgacatcat atgctttagt atagtttgtg
      301 gggagcacac taacaacagc agctttaatt tctacaaatg aagcttggtg ttccgccata
      361 atgtctaaac ttaagacatc tttatcacta atatccacaa cttcataagt taaagctggg
      421 tcatgtggca caaagtattc aacatcaacg agttcaaatg tgccaaagta gtttttaaaa
      481 taaccaccga cggtgtaaga attaccaaca gtaacaggga ataagtcatt atcttgtttg
      541 tagatatagt atccttcagt accattttgg ataaagtgtt caacagtacc ttcggctgtt
      601 actggagcgg cgtaagtgac aacaccacgg actttaaaag gagtgccgtc ttccgtattt
      661 ttattgacga aatcagcgtg tgagctatac gccatttctt cccagttttg ggtaattgcg
      721 tcttcgacga cttcaagcgt aaagactttt ttaacacttt ctttaacagc acttgttgcc
      781 gtaatattta ctgtgccaag cgccacacca gtgactaaac cagtggcatc cacggttgct
      841 tttgtttcgt cactactaga ccaattaact tcttggctag cgtcaaccgg taaaacggtg
      901 gcatttaatt taagtgtttt atcaacactg acagttgtta agtcatcaac tgaagtaata
      961 aaaatttcag ttggatcagg agcgacgatt acttttggcg taatcacaag tggtacttgg
     1021 gcagtaactt cggctttaac agtggaggta gcaatgacaa agacattacc ttcactaaca
     1081 ccagttactt ctccgctttc agtgactgtt gctttagtgg catcgcttgt cgaccaagtg
     1141 acactttgcg gtgcgccttc tggtttaaca gtaacattaa agtaagcaga ttcatcaacc
     1201 gctaacgagc ccggcccact gattgtaata ccagtaggag ttacttcttc actaacacta
     1261 gttacgggcg cttcactagt gacgactgca ctactgctac tatcaccaca accacctaaa
     1321 agtaaggcaa taagtgggat caataataat tttcttcgtt tcataaacat ct
//
LOCUS       DAOSEX010000258         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_101574_length_1366_cov_2.974828, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000258 DAOSEX010000000
VERSION     DAOSEX010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101574_length_1366_cov_2.974828"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..489)
                     /locus_tag="PK612_04505"
     tRNA            complement(399..489)
                     /locus_tag="PK612_04505"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(453..455),aa:Ser,seq:gct)
     gene            complement(533..>1366)
                     /locus_tag="PK612_04510"
     CDS             complement(533..>1366)
                     /locus_tag="PK612_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOH95401.1"
                     /translation="KTEKEKFAGAEQTFTIEALMPDGKALQCGTTHYFGDGFARVFNI
                     QFQDRNNTLQYVHQTSFGVSTRLIGSVIMAHGDDNGLSLPPRLAPVQVIIVPIQQNKP
                     GIMPKALELVSRLKAIGVRVKIDDSDKSPGWKFSEHEMKGIPLRLEIGPRDLKAEVLS
                     GSIRHSHEKISFADKDLEEVVPALLEKIHEDMYQKKLTDLLNHTIEVRSIEELEDALN
                     NKLAYAKMMWSGDQEDLDVIKEKYQATARALPFNQTPFAKKCLISGKPAKYVVIIARA
                     Y"
BASE COUNT          403 a          253 c          216 g          494 t
ORIGIN      
        1 ctactattat ttgtattagc tttttggcgc tttacaattt tgggaaactt gaaacttgtc
       61 catactcaaa cagtaaataa caaaagtaat attgagtgtt gtttgcataa ttcgtttaat
      121 atcatcttaa ctcctattta ttacccttaa tgtttttttt gttttccttc aatttcgcat
      181 ttttcgatta ttcaatattg tttaatctct tttcatattg ttttttgaag aaattcagtg
      241 aaatcttatc caatatataa taataataaa gacgctataa ttcttaattt ttatttttgt
      301 taaaatgttt atttaaaaca tatttatcac atttttgttt gtggtttaaa ttcaaaataa
      361 aaaaaagcag attttcatca aatctgctga ttaactaatg gcggagtagg tgagactcga
      421 actcacgcgc cagttacccg acctacttcc ttagcaggga agccccttca ccaacttggg
      481 tactactccg tcttgctaaa taatatcaca tgcttaatat tttgtaaatt aattaatagg
      541 cacgagcgat gattacaaca tatttagcgg gtttcccact gattaaacac tttttagcaa
      601 atggagtttg attaaaaggt aaggctctag cggttgcttg atatttttct ttaatcacat
      661 ctaagtcttc ttgatcacca ctccacatca tcttagcgta cgctaatttg ttgttaaggg
      721 catcttcaag ttcttcaata cttcttactt caatggtgtg attaagtaaa tcagtaagtt
      781 tcttttgata catatcttca tgaatctttt ctaagagtgc cggcactact tcttctaaat
      841 ctttatcggc aaaactaatt ttttcatggc tatgacgaat tgaaccactt aaaacttccg
      901 cctttaggtc gcgcggacca atttctaagc gcagcgggat acctttcatt tcatgttcac
      961 taaatttcca gcctggtgac ttatcggagt catcaatttt aactctaaca ccaattgctt
     1021 ttaagcgact gacaagttct aaggcttttg gcataattcc tggtttattt tgttggatag
     1081 ggacaatgat aacttgaaca ggagcaagcc gtggtggtag gctaagccca ttatcatcgc
     1141 catgcgccat aataacactg ccgattaaac gcgttgaaac accaaaacta gtttgatgca
     1201 catattgtaa agtgttatta cgatcttgga attgaatatt aaaaacacgg gcaaaaccat
     1261 caccaaagta gtgagttgta ccacattgta aagctttacc atctggcata agtgcttcaa
     1321 ttgtaaaagt ttgttcagcg ccggcaaatt tttctttctc ggtttt
//
LOCUS       DAOSEX010000259         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_102509_length_1358_cov_2.526477, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000259 DAOSEX010000000
VERSION     DAOSEX010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102509_length_1358_cov_2.526477"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PK612_04515"
     CDS             <1..785
                     /locus_tag="PK612_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOH95402.1"
                     /translation="IRRETARRTIELLLKDGRIQPSRIEEVVEKATQELDEIIIKAGK
                     DALAELGIPNVHKDMIPFIGRLKYRTSYGQSALKHSIEVAHLCGIMAAELGLDITRAK
                     RAGLFHDIGKSADFELEGSHVEVGARLAKKFNEPDYVIDAIESHHGDKESRFIFSQLV
                     IAADTLSAARPGARTETLENYIKRLEKLEEICKSYDGVQTSFAMQSGREVRVMVHPDK
                     VDDIMVNKLVRDIRERIENEMTYPGQIKVSVIREYRASDVAK"
     gene            788..>1358
                     /locus_tag="PK612_04520"
     CDS             788..>1358
                     /locus_tag="PK612_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOH95403.1"
                     /translation="MVKILFIGDIVGKIGRETLAHFLPILKREHDFDLIIANGENTTH
                     GRGLTKKHYEELVNLGIDVITLGNHFDDRPELKYYITEVHNLVRPLNLIENYPGEGSV
                     VVTTKKGDKVRVTNVLGQAFMKEIITSPYANLKMLNADTEKMIHIVDYHAEATAEKMT
                     IGWEFDGAVSAVLGTHTHVQTRDARILPKG"
BASE COUNT          446 a          233 c          298 g          381 t
ORIGIN      
        1 caattcgccg tgagaccgct agacggacga ttgaactatt actcaaagac ggccgaattc
       61 aaccaagtcg tattgaagaa gttgtggaaa aagcaacgca agaacttgat gaaattatta
      121 ttaaggctgg taaagacgct cttgccgaac ttgggattcc taatgtccac aaagatatga
      181 ttccatttat tggccgctta aaataccgca cgagttacgg acaaagtgca cttaaacata
      241 gtattgaagt cgcccattta tgtggcatta tggctgctga attagggctt gatattacgc
      301 gcgctaaacg tgcaggatta ttccatgaca ttggtaaatc agcggatttt gaacttgaag
      361 gaagtcacgt tgaagttgga gcaagactag cgaaaaaatt caatgaacca gattatgtta
      421 ttgatgcaat tgaaagccac cacggtgata aagaatcgcg ctttatcttt agtcaattag
      481 tcatcgctgc tgatacatta agtgcagcgc gaccaggtgc tcggactgaa acgcttgaaa
      541 attacattaa acgtttagaa aaacttgaag aaatttgtaa atcgtatgac ggtgtgcaaa
      601 ctagctttgc aatgcaatca ggccgtgaag tacgcgtgat ggtccatcct gataaggtgg
      661 atgatattat ggtcaataaa ctcgttcgtg atattcgtga acggattgaa aatgaaatga
      721 cttatcctgg tcaaattaaa gtgtcggtta ttcgtgaata ccgtgcaagt gatgtagcaa
      781 aatagtaatg gtgaaaattt tatttattgg tgatattgtt ggcaaaatcg ggcgagaaac
      841 tctcgcccat ttcctgccta tacttaagcg tgaacatgac tttgatttaa ttatcgctaa
      901 cggcgaaaat acaacgcatg ggcgcggatt aacgaaaaaa cattatgaag aattagtaaa
      961 tcttgggatt gatgtcatca cacttggtaa tcattttgat gatcgtccag aacttaaata
     1021 ttacattacc gaagtgcata atttagtgag accattaaac ttaattgaaa attatcctgg
     1081 tgaaggatct gtcgttgtca caacgaaaaa aggtgacaaa gttcgcgtca ctaatgtatt
     1141 aggacaagca tttatgaaag aaatcattac tagtccgtac gctaatctta aaatgcttaa
     1201 cgctgataca gaaaagatga ttcatatcgt tgattatcat gcagaagcta ctgcagaaaa
     1261 aatgacgatt ggctgggaat ttgatggtgc agtaagtgct gttttaggta cacacactca
     1321 tgtacaaacg cgtgacgccc gtattttgcc aaaaggta
//
LOCUS       DAOSEX010000260         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_103062_length_1353_cov_3.757319, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000260 DAOSEX010000000
VERSION     DAOSEX010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103062_length_1353_cov_3.757319"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1280
                     /locus_tag="PK612_04525"
     CDS             288..1280
                     /locus_tag="PK612_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95404.1"
                     /translation="MKLRNLLWLPIAAMLLNSCALGKKVEPSVAQERALAATKANEEA
                     EIDTQAITSKSEFSDTIKSYEGETVVTETITSKFESSFKAKGLHGDTPEFAATYKEMM
                     EEKVDGVETYYVNHEEAFYLKDSWLFGDFSTTVRERGETETEENKGKVKIVPIDFKVV
                     SVFDYLSGFSAGEYQFGAPNEEELAILFQVASEIEAKELFGKLTITYKLDVKSLVLIM
                     LLAFGLDLDAMTEEEREAFDTELNTMVEEMSKVMSINKYQMTLGISKDGYVEKIRTDI
                     DMVTNEYNEEVEPKVLVASEELKTFTESTMEINKKLTIKYPNIDDYPEAQPIIR"
BASE COUNT          506 a          192 c          257 g          398 t
ORIGIN      
        1 aactgatccg caaggtatgg gtgaagatcc tgaatatgtc atagtagtga aatcaaaagg
       61 atttaccgag attaatacaa aacttaatga aaaagtagca attaagtttc cagattttag
      121 tgattatgtt gaacggtcat tgatgtaaat aaataatgtc attagaagag tcgccgctgt
      181 gcgacttttt tattaatcaa aattgttatt tttatatgtt tctttacaat aaaaagaaaa
      241 atacaatgct ataatgtatg aatagtagaa aggaggatac ttttactatg aaactaagaa
      301 atttattatg gctcccaatt gcggcaatgt tactaaacag ttgtgcactt ggtaagaaag
      361 tcgaaccaag cgttgcgcaa gaacgcgccc ttgccgcgac taaagcaaat gaagaagcag
      421 aaattgatac gcaagcgatt acgagcaaaa gcgaattttc agacacaatt aaaagttatg
      481 aaggtgaaac tgttgtaact gaaacaatta cttcaaaatt tgaatcgtca tttaaagcaa
      541 aaggcttaca tggtgataca ccagaatttg ccgcaactta caaagaaatg atggaagaaa
      601 aagttgatgg cgttgaaaca tattatgtaa accatgaaga agcattttat ttaaaggata
      661 gttggctttt tggcgatttc tctacgactg ttcgtgaaag aggcgaaacc gaaactgaag
      721 aaaacaaagg aaaagttaaa atagttccaa ttgactttaa agttgtttcc gtgtttgatt
      781 acttatctgg cttctcggca ggtgaatatc aatttggtgc tcctaatgaa gaagaacttg
      841 ctatactctt tcaggttgct tcagaaattg aagccaaaga actttttggc aaacttacaa
      901 ttacctataa gttagatgtt aaatcactcg tgcttattat gttattagcc tttggtctag
      961 atttagatgc aatgactgaa gaagaacgtg aagcatttga cactgaactt aatacaatgg
     1021 ttgaagaaat gagtaaagtt atgtcaatta ataaatacca aatgaccctt ggtatttcta
     1081 aagatggtta tgttgaaaaa atacggactg atattgatat ggttactaac gaatataacg
     1141 aagaagtcga accaaaagta ttagttgcta gcgaagaatt aaagacattt actgaatcaa
     1201 ctatggaaat taataaaaag cttacaatta agtatccaaa tattgatgat tatccagaag
     1261 cgcaaccaat aataagataa aacaattatt gctattaatg acaagagtcg ctgcggcgac
     1321 tcttttttaa attgccacac tgatgaaaaa agt
//
LOCUS       DAOSEX010000261         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_103441_length_1350_cov_2.488803, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000261 DAOSEX010000000
VERSION     DAOSEX010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103441_length_1350_cov_2.488803"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..422
                     /gene="rbfA"
                     /locus_tag="PK612_04530"
     CDS             81..422
                     /gene="rbfA"
                     /locus_tag="PK612_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOH95405.1"
                     /translation="MPKSRNLSRNESLMLRHLSEIISYKVGDPSLGLATVSAVELTRD
                     YSYAKVYISFLGDGDVEAKMEILNNASGFIRHELSQKMNIRKTPELRFIYDDTFERVA
                     KLDELLKRTKK"
     gene            complement(499..>1350)
                     /locus_tag="PK612_04535"
     CDS             complement(499..>1350)
                     /locus_tag="PK612_04535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH95406.1"
                     /translation="RMKKAAINEVANKLGANKVAFAHHIDDALETLFMNMIGGGRFAT
                     FSPQMYLERAEITFIRPLILVDEKLIIRVQKDLKLPLLPYNCPNENKTRRDDIKSLLN
                     AIYKKYPEAKTNFELMLINDKEFDLWYKDYATLINDDCVIVKVTTPAHFNDALYIRYK
                     VFVEEQNIKVEDEYDANEKDYTSLVLYVKGNKAATIRYKYDALARRAYLGRIAVLKEY
                     RGLKLGMTLITYVEQKLSVKYRPLTFHIGGQSHLLKYYESLGYKRVGESYMDAGILHY
                     HFEKKVI"
BASE COUNT          422 a          218 c          255 g          455 t
ORIGIN      
        1 atgggagcgc gttgctaagg ttgaagacgt acttaaagaa ggcgatatta ttgaaggcta
       61 tgcgatggtg gaggttaaag atgcctaaaa gtcgtaattt atcacgtaat gaatcattaa
      121 tgctccgtca tcttagtgaa attattagct ataaagtagg tgacccaagt ttagggcttg
      181 ctactgttag tgcggttgaa cttacgcgtg actatagtta tgcaaaagta tatattagtt
      241 ttttagggga tggcgatgtt gaggcgaaga tggaaatctt aaataacgcg agcggattta
      301 ttcgccatga actatcgcaa aaaatgaata ttagaaagac accagaacta cgctttattt
      361 atgacgatac ttttgaaaga gtagcgaaat tagatgaatt attaaagcgt actaaaaagt
      421 aaagttaatt attaataaga aaaataggcc tgattataaa ggcctatttt atttttaatt
      481 gacaagaagt gtctaatgtt aaataacttt cttttcaaag tgataatgta agataccggc
      541 gtccatataa ctttcaccaa cgcgtttata ccctaagctt tcgtaatatt ttaatagatg
      601 actttgaccc ccgatatgga aagtaagggg gcgatattta acacttaact tttgctcgac
      661 ataagttatt aaagtcattc ctagtttaag accgcgatat tcttttaata ctgcaatacg
      721 acctaagtac gctcttcttg ctagtgcgtc gtacttataa cgaattgtgg ccgctttatt
      781 acctttaaca tagagcacta aactagtgta atctttttca tttgcgtcat attcgtcttc
      841 gactttaatg ttttgctctt cgacgaatac tttataacga atataaaggg catcattaaa
      901 gtgtgcagga gtagtaactt taacaatgac acagtcatca tttattagcg ttgcgtaatc
      961 tttgtaccat aaatcaaatt ctttgtcgtt aattaacatc agttcaaagt tagttttcgc
     1021 ttccggatac tttttataaa tcgcgtttaa taaactttta atgtcatcgc gtcttgtttt
     1081 attttcattt ggacagttat aaggaaggag tggtaacttt aaatcttttt gcacacggat
     1141 tataagtttt tcatcgacaa gaataagtgg acggataaag gtgatttctg ctctttcaag
     1201 atacatctgt ggactaaaag tcgcgaagcg tccgccacca atcatattca taaagagtgt
     1261 ttcaagcgcg tcatcaatat gatgagcaaa ggcgacttta ttagctccta acttattagc
     1321 gacttcatta atcgctgctt ttttcattcg
//
LOCUS       DAOSEX010000262         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_103912_length_1346_cov_2.649884, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000262 DAOSEX010000000
VERSION     DAOSEX010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103912_length_1346_cov_2.649884"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..>1346)
                     /locus_tag="PK612_04540"
     CDS             complement(266..>1346)
                     /locus_tag="PK612_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95407.1"
                     /translation="LDVPADNIGGNLLIDPNREQTVHNSIYYPNVVQAPDSIIGRAYY
                     VGDAILLSTNINQNNSKFLKFTQTVTVNPNANQVVRTDAAPMVQLTAANYSQYVTDHY
                     WQRIQDIYSRSRVRSVMFGSTPNPLYAPAINAQGQWNYPVNLPAEALAPNAPRNTICF
                     KPLTGGTSGLSFFRSNQGGDQECISVYHYKRVNNQITNLKETRFAIIKGTLGNREGVY
                     IDFVIPQSMAAEGYEFVVGISSYLPATSAGFMYLSLSGTDQSGGDNNAQVQSVDFTYY
                     TSAGTFEDLTDPNYVPKKVHFDFDGVYNGNAYFNMLDYPSNDGKVYFYYDPNFVITNN
                     LTGTVGVKTSTYNSTTFPPRQETSP"
BASE COUNT          428 a          245 c          257 g          416 t
ORIGIN      
        1 gtctgggata tatttggttt gtaatttata agtaatttta gcgatatcca tttcattgac
       61 gccacgacgc gctaggcgtt ctacaattac aacatatttt ttctcatcgt tcattcgatt
      121 tcttgtttcg taatccatat ttttttacct cctaataatt atatacatat aacgaaataa
      181 agtgattcct aattaaaaaa ataattagtg ttttacttta tcacactaat taaaagttcg
      241 aaagtaaaac gataatacta ttaatttatg gagatgtctc ttgccgtggt gggaaggttg
      301 tagagttgta agtggaagtt tttacaccaa cagtgcctgt taagttattt gtaatgacaa
      361 aatttggatc ataataaaaa tatactttgc cgtcattact aggataatca agcatattaa
      421 agtaagcatt gccgttataa acgccatcaa aatcaaagtg tactttcttt ggtacataat
      481 ttgggtcagt taaatcttca aatgtaccgg cactcgtata ataagtaaaa tcaacacttt
      541 gcacttgggc attattatcg ccaccacttt ggtcagtacc agagagtgac aggtacataa
      601 aaccagcact tgtagcgggt aagtaactag aaataccaac aacaaattca taaccttctg
      661 cggccattga ttgcgggata acaaagtcaa tataaacacc ttcgcggtta ccaagtgtac
      721 ctttaataat cgcaaatcgt gtttctttta ggttggtaat ttgattatta acgcgtttat
      781 agtgataaac agaaatacat tcttgatcac caccttggtt actgcggaag aaggagagcc
      841 cagaagtccc gcccgtcagc ggtttaaaac aaatcgtatt acgcggggca ttaggcgcta
      901 gtgcttccgc gggtaaatta accggataat tccattgccc ttgggcattt atcgcggggg
      961 cgtaaagggg attaggagta ctaccaaaca ttacactacg cacacgacta cgcgaataaa
     1021 tgtcttgaat tcgttgccag taatgatcag taacatactg tgaataatta gcagcagtaa
     1081 gttgaaccat cggtgctgca tcagtacgca caacttggtt agcgttagga ttaaccgtca
     1141 ccgtttgtgt aaattttaaa aatttcgagt tattttgatt aatattagtg ctaagcaata
     1201 ttgcatcccc aacataataa gcacggccaa taatagaatc aggggcttgg acgacattag
     1261 gataataaat gctattatgc acagtttgtt cgcggttagg gtcaattagt aagttaccac
     1321 cgatattatc cgctgggaca tcaagc
//
LOCUS       DAOSEX010000263         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_103913_length_1346_cov_2.611154, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000263 DAOSEX010000000
VERSION     DAOSEX010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103913_length_1346_cov_2.611154"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PK612_04545"
     CDS             <1..825
                     /locus_tag="PK612_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95408.1"
                     /translation="GLIGMDTYNDWPSAAIDEYLLSHNVEEKVPALPNVTRSYVELTL
                     DDPNFEHYLTVFIPGTNQAEDYTAILQAVNYQVVYYEEDDYYIGLNSETTIQIDYFYV
                     TGDADYPDGMFIFIEALATNGGGTIVTPEGIPSVIDFATEDQITKRDALQSIWTTESG
                     VTMTIDKGASTVNVGNLTTQNEVGYFTNPLRIYAGQVVTFTVPAEVQINAVLLETAGA
                     WEKGDSVLAVTNSAPETVDFIAEGDNVLFTLFSPSNTLTFSLAAQARFNKITVFVI"
     gene            complement(903..>1346)
                     /locus_tag="PK612_04550"
     CDS             complement(903..>1346)
                     /locus_tag="PK612_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95409.1"
                     /translation="IIEGIAPPVEKSNGADFIAALPTPTNAALTIAVDIRAEGDTPNQ
                     CLTLYSPYKSRVLSLSPSQLNYFKTVVAGEEQRLTDGRARYEAWALHLGDALPYEYNE
                     NFGLGNTNKHTFTSDIVMLVLVIALFGLSIGFYFIKSKQNTAKKH"
BASE COUNT          434 a          231 c          254 g          427 t
ORIGIN      
        1 ggattaattg gcatggatac atataacgat tggccatcag ccgctattga tgaatattta
       61 cttagtcata atgtggaaga aaaggtacca gcgctaccaa atgtaacaag aagttatgtt
      121 gaattaacgc ttgatgatcc aaattttgag cactatttaa cagtgtttat acccggcaca
      181 aatcaagctg aagattatac cgctatttta caagcagtaa attatcaagt tgtatattat
      241 gaagaagatg actattatat tggacttaat agtgaaacca cgattcaaat tgattatttc
      301 tatgtaacag gtgacgctga ttatcctgat ggtatgttta tttttattga agcactcgca
      361 actaacggcg gcggaacaat agttacccca gaaggtattc catcagtcat tgattttgct
      421 actgaagacc aaataacaaa acgcgatgcc ttgcaatcaa tttggacaac agaaagtggt
      481 gtcacaatga cgattgataa aggtgcatcg acagtaaatg taggaaattt aacaacccaa
      541 aatgaggtag gttactttac aaatccgttg cggatttacg ctggacaagt agttactttt
      601 actgttcctg ctgaagtaca aattaatgct gtgttattag aaactgcggg cgcttgggag
      661 aagggtgact ctgttttagc ggttactaat tccgctccag aaacagtcga ctttattgcc
      721 gaaggtgata atgtactttt cactttattt tctccaagta acacacttac attctcatta
      781 gcagcgcaag ctagatttaa taaaataact gtttttgtta tttaatcaaa tagaatcata
      841 agatatttta aaagagggtt ttggccctct tttttaaata aaataagctg tttttgcaaa
      901 ttttaatgct tttttgctgt gttttgtttt gatttaataa aatagaatcc aattgataaa
      961 ccaaagagag caatgacaag gacaagcata acaatatcgc tagtaaatgt atgcttgttc
     1021 gtgtttccaa gtccaaagtt ttcattgtat tcataaggta aagcatcacc taaatgaagt
     1081 gcccacgctt cgtatcgggc gcgaccatct gttaatcttt gttcttcacc cgctacaact
     1141 gttttaaagt aattaagctg cgacggacta agcgaaagca ctcttgattt ataaggacta
     1201 tataaagtta aacattgatt aggagtatcg ccttctgccc ttatatcaac agcaatagtt
     1261 agggcggcat ttgtcggtgt tggtaaagca gcaataaaat ctgccccatt acttttttca
     1321 actggaggag caataccttc aataat
//
LOCUS       DAOSEX010000264         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_104066_length_1345_cov_1.773643, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000264 DAOSEX010000000
VERSION     DAOSEX010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104066_length_1345_cov_1.773643"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PK612_04555"
     CDS             <1..271
                     /locus_tag="PK612_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95410.1"
                     /translation="IDTDGLNLKIVKVSNNISSVVKAIKLDHQYDMTALHTIRLSHRD
                     GNVNITFDNMEKIRNLALDIPLGKIGYKGDSYENISRLLVMLHRA"
     gene            487..1242
                     /locus_tag="PK612_04560"
     CDS             487..1242
                     /locus_tag="PK612_04560"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOH95411.1"
                     /translation="MRVDNASLGKKIALKIDNETPLEFVIPYVDTNAAFINVYLGNID
                     LVKGIHYLSVLNRENTAFSRIDFIPTAKQETVYENDLLSDKLDILSRGAPFTYTEEGL
                     TSSASRGIAYLMKEGFDNAQISVDLRFDTPSSFYSSGIVVRAENDAFHPEWDDHNSLQ
                     GYYFGVKNTQAFLMKCDYQAMSQQLAINFAEVPSHEFVTLKVVANGNTLSFYVNGVEE
                     FVVIDADAKYGGKLGLYTTGVTTTFRNLKVTLI"
BASE COUNT          452 a          216 c          237 g          440 t
ORIGIN      
        1 cattgatact gatgggttaa atcttaaaat tgttaaagtt agtaataaca tttcttctgt
       61 tgttaaagca attaaacttg atcatcaata tgatatgact gcacttcaca ctattagact
      121 ttcacatcgt gatggtaatg taaatattac ttttgacaac atggaaaaga ttcgtaatct
      181 agcgctagat atcccgcttg gtaaaattgg ttataaaggg gatagttatg aaaacatttc
      241 acggctttta gtaatgttgc acagggctta agtgaccaag aatacgctaa acagaaaact
      301 ttatatgcga atgcttatga cgaaaatgtt tcaagattaa atgcttcttc gcttgttagt
      361 attaacggcg atactgaaga cgaaaattat aacggaaaga ataatagtta taaacttcaa
      421 ttatctggtg aagaatatgc aacttataag attcttgcca cgaacactga cacttatcaa
      481 atcacgatgc gtgttgataa tgcctcactt ggtaaaaaga ttgcacttaa gattgataac
      541 gagacaccac ttgaatttgt aataccatat gttgatacaa atgcagcctt tattaatgtt
      601 tatcttggga atattgattt agtaaaaggc atccattatc tttctgtgct taatcgtgaa
      661 aacacagctt ttagcaggat tgattttatc ccaactgcaa aacaagaaac agtatatgaa
      721 aatgatttat taagcgataa gcttgatatt ttaagtcgtg gcgctccgtt tacttatacg
      781 gaagaggggt taacatcaag tgcgtcacgc gggatagctt atttaatgaa agaaggtttt
      841 gataatgctc aaatcagcgt tgatcttcgt ttcgacactc cttcttcatt ttattcatca
      901 ggtattgttg tccgcgctga aaatgatgcc ttccatccag aatgggatga tcataattca
      961 ttacaaggct attactttgg agttaaaaat acacaagctt tcttaatgaa gtgtgactac
     1021 caagcaatgt cacagcaact agcgattaat tttgcggaag ttccttctca tgaatttgtg
     1081 acacttaaag ttgtcgctaa tggtaataca ctttcctttt atgttaatgg cgttgaagaa
     1141 tttgtggtca tagacgctga tgcaaaatac ggtggtaaac taggattata cacaactggc
     1201 gtaactacca catttagaaa tcttaaagtt acactaattt aatatacaaa cactaaaatt
     1261 gttagccata attttttaat taatgttatt attgtactga taccctaaat gtagacacac
     1321 aataaaatca tgtggccaca tgatt
//
LOCUS       DAOSEX010000265         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_104143_length_1344_cov_2.539953, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000265 DAOSEX010000000
VERSION     DAOSEX010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104143_length_1344_cov_2.539953"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PK612_04565"
     CDS             complement(<1..873)
                     /locus_tag="PK612_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95412.1"
                     /translation="MREAYKKIPCRITVIDLLKLTGMIFICIPHCLQRWGPSDYTSTL
                     GFSFFYSVGLSMFFFSSGIVLRRITSLKDLLIFIVKTVGVYLTSAFIFTVLSIYTLPR
                     FTLLEKDFGYWMNELYLRTDTFYWYSLVATLINIFIASAYYFVSLIKKNNLKWDIFKA
                     SLIIILTVAYSMIFLHIYNREDLGPGCLGASLVLYYLPITLFGFLFNIFKPYLLKLPR
                     QELLKGIATLVALVIYIPLLIVYRDWLPHLNGSFWTISALWICSLSGTIVYYYLLSKI
                     SHFTIAKKVSYYGFF"
     gene            1011..>1344
                     /locus_tag="PK612_04570"
     CDS             1011..>1344
                     /locus_tag="PK612_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HOH95413.1"
                     /translation="MRILLFNKKQKLYDKSGVGRAMKHQVKALTEAKIPFTTNEKDDY
                     DLVVINTIDPSAFRMARKARRQGKKVIVYGHSTKEDFMNSFMLSNQIAPFFKIHLKRI
                     YKICDIVIT"
BASE COUNT          527 a          222 c          219 g          376 t
ORIGIN      
        1 aaagaagccg taatatgata cttttttagc aattgtaaag tgtgagattt tggaaagtaa
       61 gtaataatac acaattgtcc cgctaagtga acatatccat aaggcactaa ttgtccaaaa
      121 tgagccgttt aaatgaggga gccaatcgcg atacacaatt aaaagcggta tataaattac
      181 tagcgcaact aaagtagcga tacccttaag taattcttgg cgtggtagtt taagtaaata
      241 cggtttaaat atgttaaaaa gaaaaccaaa taaagttatc ggtaaatagt aaagcactaa
      301 agaagcacct aagcaccctg gtcctaagtc ttctctatta taaatatgta aaaaaatcat
      361 tgagtaagca actgtaagta taattataag acttgcctta aaaatgtccc acttaaggtt
      421 atttttcttt attaaagaca caaagtaata tgcactagcg ataaaaatat taattaatgt
      481 tgcgacaagc gaataccaat aaaatgtatc agtgcgtaaa tacaattcat tcatccagta
      541 gccaaagtct ttttctaaaa gcgtaaagcg cggcaaagta taaattgata aaacagtaaa
      601 aataaaggcg gatgttaaat acacaccaac agtttttact ataaatataa gcaaatcttt
      661 aagtgatgtg atgcggcgga gcacaattcc gctactaaag aaaaacatcg aaagaccaac
      721 actatagaaa aaagaaaagc ctaatgttga tgtataatca cttggccccc atctttgtaa
      781 acagtgtggg atacaaataa aaatcatccc tgtaagtttt aataagtcaa tgaccgttat
      841 ccgacacggt atctttttgt aagcttcacg catagcttta actctttcta ttattaaggg
      901 tttagttccc tttaatagca ataattatag cattaaatta taaactatta acacgttcaa
      961 aaaagtatat atcgtgatat gataaaaaga gagtgaaagg agcccggagt atgcgaattc
     1021 tcttatttaa taaaaaacaa aaattatatg ataagtctgg tgtgggtcgc gcgatgaagc
     1081 atcaagtcaa agcattaaca gaagcaaaaa tcccgtttac cacaaatgaa aaagatgact
     1141 atgatctcgt cgtcattaac accattgacc ctagtgcttt ccgtatggca cgcaaagcaa
     1201 gaagacaagg aaaaaaagta atagtttatg gtcactcaac aaaagaagac tttatgaact
     1261 ccttcatgct aagtaatcaa attgcacctt ttttcaaaat acacttaaaa cgcatttaca
     1321 aaatttgtga cattgtcata acac
//
LOCUS       DAOSEX010000266         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_104183_length_1344_cov_1.923196, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000266 DAOSEX010000000
VERSION     DAOSEX010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104183_length_1344_cov_1.923196"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="PK612_04575"
     CDS             complement(<1..1192)
                     /locus_tag="PK612_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH95414.1"
                     /translation="MEKKSHNLPMPKVHSWQDVKDVYFLYITNAEDRANIEEAYNFAL
                     KKHEGQFRKSGHPYITHLIEVAFILAELTAGPSTIIAGLLHDVVEDTDVTVSEIDKMF
                     GKDVAMIVDSVTKIQRLKLSKKVDDSDFVYEDHRKIFLGMAKDIRVIIIKLADRLHNL
                     RTLYFLSHERQLRIAKETMAVYAPIAGRLGMGKIKAEMEDLSLKYIEPEAFNHVSELV
                     EQRSPNLQKALAEVSKRIADILFKNDIKFEMKFRVKSIYSIYKKMKEKDNDFERIYDL
                     LALRIITPTELNCYEILGLIHATYRPVTGRFKDYIAMPKSNMYQSLHTTIFTGDGNAL
                     EVQIRTPEMDKIADSGVAAHWAYKEGTNYNPEREQREIENKLHWLRDFVATGYETGNN
                     AKEYM"
BASE COUNT          376 a          283 c          215 g          470 t
ORIGIN      
        1 ccatatattc tttcgcatta ttaccagttt catagcccgt tgccacaaaa tcacggagcc
       61 aatgcaattt attttcaatt tcgcgttgtt cacgttcggg attataatta gtcccctctt
      121 tatacgccca gtgtgccgca accccagaat cggcaatttt gtccatctct ggcgttctaa
      181 tttgcacttc taaagcatta ccgtcacccg taaagatcgt tgtgtgtaaa ctttgataca
      241 tattgctttt tggcatcgcg atataatctt taaagcgtcc cgtaactggg cgataagtcg
      301 cgtggattag tcctaaaatt tcataacaat taagttccgt tggagtaatg attctaagcg
      361 ctaaaaggtc ataaatccgt tcaaagtcat tatctttctc tttcatcttc ttataaatac
      421 tataaatcga cttcacacga aacttcattt caaatttaat gtcattttta aataagatat
      481 ccgcaattcg tttactaact tccgcaagcg ccttctgtaa gtttggggag cgctgctcaa
      541 cgagttcact tacatgatta aaggcttctg gttcaatgta ttttagactt aagtcttcca
      601 tttctgcttt aattttcccc atccctaggc gtccagcaat cggagcgtaa accgccatcg
      661 tttctttagc aatacgcagt tgtcgttcat ggcttaaaaa gtataatgtt cgcagattat
      721 gtaatctgtc cgcaagttta ataatgataa cacggatgtc ttttgccatc cctaagaata
      781 ttttacggtg atcttcatac acaaaatcac tatcatcaac ttttttacta agttttaggc
      841 gttgaatttt cgtgacgcta tcgacaatca tcgccacatc tttgccaaac attttatcaa
      901 tctcactaac tgttacatca gtgtcttcaa cgacatcatg cagtaaacca gcaataatcg
      961 tacttggacc cgctgttagt tcagcaagaa taaaggcaac ctcaattaaa tgcgtaatat
     1021 aaggatgacc gcttttacga aattgccctt catgtttttt aagcgcaaaa ttatatgctt
     1081 cttcaatatt agcgcggtct tcagcattag taatatagag aaaatagaca tctttgacgt
     1141 cttgccacga atggactttt ggcattggta ggttatgact ttttttctcc atactatgcg
     1201 tttcctttct tattaattat aacaaattaa taatcgcttt gattatgctt tacttaataa
     1261 gctgctttta ataaaaaaag actctctgtt gagagtcgag ttcgtcagta atattcttaa
     1321 ttacttatct tcggtttggg cttt
//
LOCUS       DAOSEX010000267         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_104233_length_1343_cov_3.607919, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000267 DAOSEX010000000
VERSION     DAOSEX010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104233_length_1343_cov_3.607919"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PK612_04580"
     CDS             complement(<1..295)
                     /locus_tag="PK612_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95415.1"
                     /translation="MNHYVVKQTAGKRFLAGFLDTIFVIILALFLYIPARTIVDNNGL
                     IEVFVKITQIQLKSGLYDVEGIDNAVPYIINDVEKFPKALYTFYVDENLETGEI"
     gene            complement(295..>1343)
                     /locus_tag="PK612_04585"
     CDS             complement(295..>1343)
                     /locus_tag="PK612_04585"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOH95416.1"
                     /translation="TGAVLIDLVIMYLLMNVLWFAIHPIYNKVYDIDNVVTAHERDKK
                     RSNLVVLNEPILDDESNIDLVKVESNQLPETKRGEGVFKFYTVFLRSEQEAKLAAGEI
                     DNTPENNAMFTFDWYLENVIKPDTEESIYLRIPTDEHLGAEGAVSSEETAEPEYDTFR
                     PAGLDFKADLTAEDINKFNNRIYNQAVNAFNLRPNMTIINKVNIQENIILMVLGSTVV
                     FLVFPLFFKHGQTLGKKALKLGVTTRHGYTVTVPWLLLRYFAFLFINLLSNLLIPIIL
                     PFISLTIMTFSKERRSAHDFIANTKVIDLQNSTVYKSEAEFLEAQKRYNPRLEAEDFN
                     EEVFSERFDEESGA"
BASE COUNT          427 a          259 c          215 g          442 t
ORIGIN      
        1 caatttcgcc tgtttcaaga ttttcatcaa cgtaaaatgt gtagagtgct ttagggaact
       61 tttcaacatc attaatgatg taaggtactg cattatcaat cccttcaacg tcatatagtc
      121 cgctttttaa ttgaatttgt gtgattttaa caaacacttc gattaagcca ttattgtcta
      181 ctatagtgcg cgccggaata tataaaaaaa gcgccaatat aatgacaaat atcgtatcaa
      241 ggaaacccgc aaggaagcgt ttacctgctg tttgctttac gacataatgg ttcattaggc
      301 gccactttct tcatcgaagc gttcactaaa tacttcttca ttaaagtctt cagcctcaag
      361 tctaggatta tatctttttt gtgcttctaa gaactcagct tcacttttat atactgtcga
      421 attttgtaaa tcaattactt tcgtattagc gataaagtca tgcgcactgc gacgctcttt
      481 actaaaggtc ataattgtga gtgagataaa cggtaaaata attggaatta agagattact
      541 taataagtta ataaataaga aagcaaagta acgaagtaat aaccacggca cagtaactgt
      601 gtaaccatga cgcgtcgtta ctcctaattt taaggccttt ttgccgagtg tttgaccgtg
      661 tttaaagaaa agcgggaaca ctaaaaagac gacagtgcta ccaagcacca tcaagattat
      721 attttcctga atattaacct tattaatgat tgtcatattc ggtcgtaaat taaaagcgtt
      781 cactgcttga ttataaatgc ggttattaaa cttattaata tcttccgcag ttaaatccgc
      841 tttaaaatct aatcccgctg gacgaaaagt gtcgtactcc ggttcagcag tttcttcact
      901 actaaccgcg ccttctgctc ctaaatgttc atcagtggga atgcgtaaat aaattgattc
      961 ttccgtatca ggtttaatga cattttctaa gtaccaatca aatgtaaaca ttgcattatt
     1021 ttcaggtgta ttatcaattt cacccgccgc taatttcgct tcttgttcac tgcgcaaaaa
     1081 gactgtgtaa aacttaaaga caccttcccc gcgttttgtt tctggtaatt gattactttc
     1141 aactttaact aagtcaatat tgctttcatc atcaagaatc ggttcattaa ggacgactaa
     1201 gttactacgt tttttatcac gctcatgtgc ggtcacaaca ttatcaatat cataaacttt
     1261 gttataaatt ggatgaatgg caaaccataa tacattcatt aataaataca taatgacaag
     1321 gtcaattaaa actgcaccag tac
//
LOCUS       DAOSEX010000268         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_104567_length_1340_cov_6.771984, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000268 DAOSEX010000000
VERSION     DAOSEX010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104567_length_1340_cov_6.771984"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PK612_04590"
     CDS             complement(<1..887)
                     /locus_tag="PK612_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOH95417.1"
                     /translation="MTKTIEVIMPHKIDTSLTKTNKTKLRVAAYARVSTDEDDQVNSF
                     KSQIDEYTERITSNPDWDFIGMFADKGITGTQTKKRPEFMRMVELGMERKIDLILVKS
                     ISRFARNTVDVLTTVRELRNRGCIIFFEKENIRSDDTKIDFVLTVLSSVAQEESRSIS
                     TNVKWSIEKKFKNGIAHVTNIYGYRKGENGDLIIEESEATIVRLIFSLFIEGYNINDI
                     VKILNDRKIPTLKGKSWLYSTVRGLLSNEKYMGDAILQKTCTVDYLTHKQVKNDNIEP
                     KYYVTNHHEGIIKRDEYETV"
BASE COUNT          372 a          267 c          254 g          447 t
ORIGIN      
        1 acggtttcat attcgtccct tttgatgatg ccttcatgat gatttgtcac ataatacttc
       61 ggttcgatat tatcattctt gacttgcttg tgcgttagat agtcaacggt acaagtcttc
      121 tgtaatatgg catcacccat atatttctca ttactaagaa gtccacgaac agtggagtat
      181 agccacgatt tacctttaag cgttggtatt tttcggtcat tgagaatttt gacaatgtca
      241 ttgatgttat agccttcgat aaacaaagag aagatgaggc gaacaatagt ggcctcgctc
      301 tcttcaataa tcaaatctcc gttttcgccc ttacgataac cataaatatt ggttacgtga
      361 gcgatgccgt tcttgaattt cttttcgatg ctccatttaa cgttggtcga tatcgagcga
      421 ctctcttctt gggcaacact tgatagaacc gtaagaacga agtcaatctt ggtatcatcg
      481 ctacggatgt tttccttttc aaagaaaatg atgcatcccc ggtttcttaa ctctctaacg
      541 gtcgtgagaa cgtcgactgt attacgtgca aaacgagaaa tggacttaac tagaattaag
      601 tcaattttgc gttccattcc taactcaacc attcgcatga actctggccg tttcttggtc
      661 tgcgtaccag tgatgccttt gtccgcaaac ataccgataa aatcccaatc gggattagag
      721 gtaatgcgtt cggtgtattc atcgatttga gacttgaatg aatttacttg atcatcttca
      781 tcagtggata cacgtgcata agccgcaacc cgcaattttg ttttgttagt ttttgtaagt
      841 gaagtgtcga ttttatgcgg cataatcacc tcaatagttt tcgtcatgtc ttgaattact
      901 cctttcgtaa acgagtagta ttactcgctt gtttgtttta taacttaaat atttgattac
      961 cgataatgaa atcgcgcatt tgaagatatt cttcaatact taaaaaaccc ttcacaaaaa
     1021 gaagggttaa agcatgtcga attagtttgt gttgaacatc tggattcatt ctttgctcct
     1081 tttaagaaca aggaatgact tatttaatct tgagccaggt tcattctatt ttatgtacgt
     1141 gcgtaataag agggtcaaac tgaagactac tgttcaataa agcgtccagg aatggtctcg
     1201 acatagtcgc cgacatggcg agctagtatg aagagatcct ttcgtttcat gctgactgga
     1261 ttgtccttat taaacaaaac aaggacgatt ccgtttttat ctgacggatc accgacaaat
     1321 aataactcag ccactttctt
//
LOCUS       DAOSEX010000269         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_104705_length_1339_cov_3.623832, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000269 DAOSEX010000000
VERSION     DAOSEX010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104705_length_1339_cov_3.623832"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..913)
                     /locus_tag="PK612_04595"
     CDS             complement(170..913)
                     /locus_tag="PK612_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95418.1"
                     /translation="METKKKRTINIILKISIIVAAIIGIISQQLHGLLIYTGHVPFMY
                     FTTQSNILVAVCMGILLYYDLTEKEVPRWVLIFQHITVTAVTLTFLVFAIFLGPFIPD
                     LSVGYFYSVQNLTLHNIVPILAISQYLYDDNRDIPKYAKYLGLSSGFIYVIFVYIVHL
                     CGATFGATEFPYFFLDYKEFGWLTINEGKIGIIYWWIVIILLLFGISALLYYLKKKSA
                     ERKSIPVAIFNSLIILSIIFIIINIIIKI"
     gene            996..1072
                     /locus_tag="PK612_04600"
     tRNA            996..1072
                     /locus_tag="PK612_04600"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1030..1032,aa:Thr,seq:cgt)
     gene            complement(1132..>1339)
                     /locus_tag="PK612_04605"
     CDS             complement(1132..>1339)
                     /locus_tag="PK612_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95419.1"
                     /translation="LTSGAVALTHKRQALHGIFSGTYVVDARTSKIFNNINDEEEYFS
                     LSVEERELNKNYYQEKPYVPLKKL"
BASE COUNT          473 a          210 c          226 g          430 t
ORIGIN      
        1 taaatcaagg cgatttataa gcacaggtaa atccatcacg gtattgacaa gaattgatcc
       61 gatgatatta tcaattagct tacagctagt aagagtaaat aatgtgacaa gcgccaatgg
      121 gactactttt aatttacgaa gtttcataat aggaccccct aaaccttctt tatattttaa
      181 ttataatatt aattattata aaaatgatag ataaaataat tagactatta aaaattgcga
      241 ctggaatact tttgcgttca gcactttttt tctttaagta atataagagt gcactgattc
      301 caaatagtaa taaaattatt actatccacc aataaataat gccgatttta ccttcattta
      361 ttgttaacca tccaaattct ttatagtcaa ggaagaagta aggaaactct gttgcgccga
      421 aagtagcgcc acaaaggtga acaatataca caaaaattac ataaataaat ccgctagaaa
      481 ggcctagata tttagcatat tttggaatat cacgattatc atcatataaa tattggctga
      541 tagcaagaat cgggacaata ttatgtaatg ttaaattttg cacggaataa aaataaccaa
      601 cgctaagatc aggaataaat ggaccaagga aaatagcaaa cactaaaaag gttaatgtaa
      661 cagcggtaac tgtaatatgc tgaaagatta aaacccagcg cggaacttcc ttttcagtta
      721 agtcgtaata tagtaaaatt cccatacaaa cggcgacaag aatattactt tgggttgtaa
      781 agtacataaa tggtacgtga cccgtgtaaa ttaataaacc atgcagttgc tgtgatatta
      841 ttccgataat tgcggcaacg ataatactaa tctttaagat aatgttaata gtgcgttttt
      901 tcttagtttc cataaaaaga ttatactaca aatcaagata ttatcttgat gtttttattg
      961 caatattgtg ataaagtcgg tatgattaag actaggccga cgtagctcaa tctggcagag
     1021 caactgaatc gtaatcagta ggttgcgggt tcaattcctg tcgtcggcac cagtcaaaat
     1081 gaaaatgtcc cacgcatctg ggactttttt attaattaaa agtaatgttt attaaagttt
     1141 tttaagggga acgtagggtt tttcttgata ataattttta ttaagttccc gttcttcaac
     1201 acttagtgaa aaatattctt cttcatcatt aatgttatta aatatttttg aggttcttgc
     1261 atcaacgaca tatgtgccac tgaaaatacc gtgtagcgct tgtcttttat gtgtaagtgc
     1321 gaccgccccg cttgtgagt
//
LOCUS       DAOSEX010000270         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_106775_length_1322_cov_3.187845, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000270 DAOSEX010000000
VERSION     DAOSEX010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106775_length_1322_cov_3.187845"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PK612_04610"
     CDS             complement(<1..740)
                     /locus_tag="PK612_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOH95420.1"
                     /translation="MKKLTSNDIRKMWLSFFKSKNHAVVEGASLIPVNDKTLLWINSG
                     VAALKQYFDGSKVSADKRLTNVQKSIRTNDIENVGKTNRHHTFFEMLGNFSIGDYFRT
                     EAITWGFEFLTSKDYLALPLEKLYFTYHPSDTETRDLWISLGVPKSHVIANEDNYWEI
                     GEGPCGPNTEIFFDLGEKIDARGPELLASDIDNDRFLEIWNIVFSQYNAKAGVKRENY
                     EELPSKNIDTGAGLERIASVLQEVPSNF"
     gene            complement(970..>1322)
                     /locus_tag="PK612_04615"
     CDS             complement(970..>1322)
                     /locus_tag="PK612_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOH95421.1"
                     /translation="PENKRWYVCKKDVVNNILYVANDDEDEHLKSDKITLTDVNFINE
                     ELKSGTPLNVKLRYRQKDLPATYYKNKDGAYLLFPKPYLAVTPGQAAVFYIDEVCLGG
                     AIIGDLFYKGKKIN"
BASE COUNT          398 a          278 c          200 g          446 t
ORIGIN      
        1 tcaaagttac ttggcacttc ttgtaataca ctagcaatcc gttctaaacc agcgccagta
       61 tcgatgtttt tactcggtaa ttcttcgtaa ttctcgcgct ttactcccgc tttagcgtta
      121 tattgactaa acacaatatt ccaaatttca aggaacctgt cattatcaat atcacttgct
      181 aataattctg gaccacgcgc atcaattttt tcccctaaat caaagaaaat ttcggtattg
      241 ggaccacacg gtccttcccc aatttcccaa taattatctt cattagcgat aacatgactc
      301 ttcggcacac ctaaactaat ccataagtca cgcgtttctg tgtcacttgg gtgataagta
      361 aaataaagtt tctcaagcgg taaagctaag taatctttac tcgttaaaaa ttcaaagccc
      421 caggtaattg cttccgtacg gaaatagtca ccaatactaa aattacctaa catctcaaaa
      481 aaggtgtggt gacgatttgt tttaccgaca ttttcaatat cattcgtgcg aatacttttt
      541 tggacatttg ttaaacgttt atccgcactt accttactac catcaaaata ttgtttaagc
      601 gctgcgacgc ccgaattaat ccaaagtagt gtcttatcat taaccggaat caaactagcg
      661 ccttccacta cggcatgatt tttgctttta aaaaaggaca gccacatttt gcgaatgtca
      721 ttacttgtta attttttcat aaaaaacctc aactaatata ggaacgactc tcgccgcggt
      781 accatcctaa ttcatcgttg tgatgcgctt tattttaatt ttgtaacggt aattaaccgg
      841 cgatgattag tcgctcgaag gaagtggcac gttttacttt tgagtccttt caccaactaa
      901 ctctcgcttt taagtttgta aaacttagtt tccttattga tttcgataat attatagcat
      961 agttaacgat tagttaatct ttttgccttt ataaaataag tcaccaataa tagcaccgcc
     1021 aagacacact tcgtcaatat agaaaactgc tgcttgacct ggggtcacgg ctagataagg
     1081 tttggggaat aataagtagg caccgtcttt atttttataa taagtggcag gcaagtcttt
     1141 ttgccgataa cggagcttaa catttaaggg agttccactc tttaattctt cattaataaa
     1201 attaacatca gtcaaagtaa tcttatcact ttttaaatgt tcatcttcat catcattagc
     1261 gacatataaa atattattta ccacatcttt tttacataca taccagcgtt tattttctgg
     1321 gt
//
LOCUS       DAOSEX010000271         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_107264_length_1318_cov_2.870942, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000271 DAOSEX010000000
VERSION     DAOSEX010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107264_length_1318_cov_2.870942"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..>1318
                     /gene="dcm"
                     /locus_tag="PK612_04620"
     CDS             149..>1318
                     /gene="dcm"
                     /locus_tag="PK612_04620"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR00675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOH95422.1"
                     /translation="MLKVIELFAGVGAQVQGLKDANVPHEIVAISEIDKYALSVYEAL
                     HGAVNNLGDIRSIESLPRADLWTYSFPCTDISISGRMKGFEKESGTGSSLLWEVERLL
                     EKARENGNLPKYLLMENVKNIVSKRFKPLFEEWIDYLSSLGYKSFYKVLNAKDYGVPQ
                     NRERCFMVSFLNYDGDFSFPNKVPLETKLSDLLEKDVAEKYFLSEKLITNFSSMENRN
                     GFIRGLRFRPQFRDAPFGWTITTNPGSRATDNFIIEPIPCALRGRREEDGKIHQHLEI
                     KKDGITNTLTTVTKDNLILEGLDNSKFMIPQAVKKGYALAEVGDGVYTNRCEFKRGVV
                     QKKMIPTLKTTVSDIGVVVNDPQDLISIRRLTPRECWRLMGWKDESIDIAFSTKVS"
BASE COUNT          439 a          210 c          298 g          371 t
ORIGIN      
        1 gaagggcgag tgtgtgacga atgtaatatc gctaaagtac taccagcacg catcttatta
       61 atgaataaaa ataaatagct aaaacggcta ttaactgagc aagaggtacc aattggtgcc
      121 ttttttctta ctcaaaagga gggttcatat gttaaaagtg attgaactct tcgcgggagt
      181 aggggcgcaa gtgcagggat taaaagacgc taatgttcct catgaaatag tcgcaataag
      241 cgaaattgat aagtatgcgc taagtgtgta tgaagcctta cacggagcag ttaataatct
      301 tggagatata cgaagcatag aatccttacc aagagcggac ctatggactt attcgtttcc
      361 gtgtactgat atttctattt ctggtcggat gaaagggttt gaaaaggaaa gtgggactgg
      421 ttcatcgctt ttatgggagg tagagcgact tttagaaaaa gcacgagaaa acggcaattt
      481 accaaaatat ctcttaatgg agaacgttaa aaatattgtg tcgaaacgct ttaagccttt
      541 atttgaagaa tggatcgatt atctttcatc gttaggctat aaatcgttct ataaagtgtt
      601 aaatgctaaa gattacggtg ttccgcagaa tcgagaacgg tgcttcatgg tttcgtttct
      661 taattacgat ggtgatttta gctttcctaa taaagtacca ttagaaacaa aactaagtga
      721 tttactcgaa aaagatgtcg cggaaaaata ctttttaagt gaaaaactta ttactaactt
      781 ttcaagcatg gagaatcgta atgggtttat tcgtggactg cggtttagac cacaatttag
      841 agatgcgccc tttgggtgga cgattactac aaatccaggt agtagagcaa ctgataattt
      901 catcattgaa ccgattccgt gcgccttacg tggacgaaga gaagaggatg gaaaaatcca
      961 ccagcatctt gaaattaaaa aagacgggat tacaaatact ctcacaactg taactaaaga
     1021 taaccttatt cttgaaggat tagacaattc aaagtttatg attccacaag cggtgaaaaa
     1081 agggtatgcc ttagcggaag taggggacgg tgtttatacg aatagatgcg aatttaaacg
     1141 aggtgtcgta caaaaaaaga tgattccaac attaaagact acagtctccg atattggcgt
     1201 tgtcgttaat gatccacaag acttaatttc aatacgacga ttaacaccgc gagagtgttg
     1261 gcgccttatg gggtggaaag atgagagtat cgacattgct tttagcacta aagtaagt
//
LOCUS       DAOSEX010000272         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_108647_length_1307_cov_3.007188, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000272 DAOSEX010000000
VERSION     DAOSEX010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108647_length_1307_cov_3.007188"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="PK612_04625"
     CDS             complement(<1..106)
                     /locus_tag="PK612_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase SNF2"
                     /protein_id="HOH95423.1"
                     /translation="MQYEPHEYQTFATNYIETHPISALLVDMGLGKTVI"
     gene            complement(87..371)
                     /locus_tag="PK612_04630"
     CDS             complement(87..371)
                     /locus_tag="PK612_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019260865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HOH95424.1"
                     /translation="MGKETKIEKKLVQEVKKAGGLALKLVSPGYVGVPDRLILIAFGK
                     IGFVEVKAPNQKPRKIQMRRHKELQKLGFKTYVLDDEKEIGGIIDAIRTT"
     gene            complement(514..>1307)
                     /locus_tag="PK612_04635"
     CDS             complement(514..>1307)
                     /locus_tag="PK612_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HOH95425.1"
                     /translation="GKSTFFNSISHVIGDYHGSIASDILTTGIKRDKQADLAELRGKR
                     LVIAAETKVGDSLDESTIKRMCSTDKINACKKYKDPFDFVPSHTLVVYTNNLPKVNTI
                     DDGTWRRIIVIPFKHKFTGTEDIKNYAEVLVEEAGEYILYWLIEGAKKAIDNKFHIEP
                     PAEVIDAIKGYKEQSDTITMFLQEKCDTSDKKAECPSGELYAKYRAYCYEMGEKARST
                     TDFNVVLSKNGFIKVKKNSRFYIKGISILPIDLSSAAEDFEELLK"
BASE COUNT          330 a          286 c          215 g          476 t
ORIGIN      
        1 taataaccgt cttccctaac cccatatcaa cgaggagtgc actaatcggg tgcgtttcaa
       61 tgtagttagt ggcaaatgtt tgatattcat gtggttcgta ttgcatcgat aatcccccct
      121 atttcttttt catcatcaag tacataagtt ttaaacccaa gtttttgtag ttccttatga
      181 cgcctcattt gtatcttgcg tggtttttga ttaggtgctt ttacttcgac aaagccaatc
      241 ttgccaaacg ctattagaat taagcggtca ggaacaccta catatcctgg actaacgagt
      301 tttaaggcta aaccaccagc ctttttaact tcttgcacca atttcttctc aattttcgtc
      361 tcttttccca tgcgacctct ttccaatact ttagtattga tgagattgat gaagtcgttt
      421 ccgtactttt ctatatataa tttttattat tatttttttc cttatatgaa taagttagga
      481 tatgacctca tcgacctcat tgttttgaac tttttacttt agtaactcct caaaatcttc
      541 ggctgcagag gagaggtcga ttggcaaaat tgatatccct ttaatgtaaa atcggctgtt
      601 tttctttact tttataaacc cgtttttact aagcaccaca ttaaaatctg ttgtgctccg
      661 cgccttttca cccatttcat agcaataggc acgatacttg gcgtaaagtt ctccgcttgg
      721 gcattcagcc tttttatcac tcgtatcgca tttctcttgg agaaacatgg taatggtatc
      781 gctttgctct ttatagcctt taatggcgtc tattacctct gctggcggtt caatatgaaa
      841 cttattatca atcgcttttt tggcgccttc aattaaccaa taaaggatat attcgcccgc
      901 ctcttccact agcacttccg catagttttt aatgtcttct gtgccagtaa atttatgctt
      961 aaacggaata acaataatcc ttcgccaagt gccgtcatca atcgtattta ctttcggtaa
     1021 attgttcgtg tagacgacta gcgtatgact tggcacaaaa tcaaatggat ccttatattt
     1081 tttacaagcg tttattttat cggtcgaaca catgcgctta atggttgatt catcaagact
     1141 gtcgccaact ttcgtttctg ccgcaattac aagccttttg ccgcgtaatt ctgctaaatc
     1201 agcttgttta tcacgtttaa ttcctgtcgt taatatgtca cttgcgattg acccatggta
     1261 gtcacctatt acgtgcgaga tagaattaaa aaacgtcgat ttcccgt
//
LOCUS       DAOSEX010000273         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_108672_length_1307_cov_2.418530, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000273 DAOSEX010000000
VERSION     DAOSEX010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108672_length_1307_cov_2.418530"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1307
                     /gene="alaS"
                     /locus_tag="PK612_04640"
     CDS             <1..>1307
                     /gene="alaS"
                     /locus_tag="PK612_04640"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOH95426.1"
                     /translation="VPSNFETDLFMPIIKELEKLSGVKYSDDIVSFCVIADHIRTSTF
                     AIADGASFSNEGRGYVLRRVLRRAVRYGQKLAMKEPFLHKLVQSVVDVMKDFYPYLES
                     KIDHVEKLIKAEEVRFLNTLNHGEELLRGALSNSKNISGEVAFKLYDTFGFPIDLTLE
                     IASENGASVDVKGFEVLLEEQRERARAARKNLNSMASQSEDLLKFDQKSKFEEQKFSV
                     TATVIGLFKDGVKVNESAEDVSAIFDVTPFYSESGGQVSDSGFVSNKELRAVVSAMSK
                     APHGQHLHHLTLDYGVIKVGDKVQLEIDGVARRSIMLNHSATHLLHRALNDVLGSEVS
                     QAGSYVGPDYLRFDFNYNTRVTDEELEKIELIVNKYISDSVPLTVKYMTLAEAQKLGA
                     KALFMEKYGDLVRVVTFARISVGLCGGTHIDNTSKIGTFVIVS"
BASE COUNT          416 a          205 c          287 g          399 t
ORIGIN      
        1 agtgccaagt aactttgaga ctgatttatt tatgccaatt attaaagaat tagaaaagtt
       61 atcgggcgtt aaatatagtg atgatatcgt tagtttctgt gttattgctg atcatattcg
      121 tacatcaaca ttcgcaatag cagatggcgc tagttttagt aatgaagggc ggggttatgt
      181 cttacgccgt gtattacgac gcgcggtgcg ttatggccaa aagctcgcta tgaaagaacc
      241 cttccttcac aaattagtgc aaagtgtagt tgatgttatg aaagacttct atccatattt
      301 agaaagtaaa atagaccatg tcgaaaaact tattaaagcc gaagaagtac gctttttaaa
      361 tacacttaat cacggtgaag aattattacg cggtgcccta tcaaatagca agaacatcag
      421 cggggaagtt gcatttaaac tctatgatac ttttgggttc cccattgatt taacactaga
      481 aatcgctagt gaaaatggcg cgagtgtcga tgtgaaaggc tttgaagttt tgcttgaaga
      541 acaacgcgaa agagcacgtg ctgcgcgtaa aaatttaaat agcatggcta gtcaaagtga
      601 agacttatta aagtttgatc agaagtcaaa atttgaagaa caaaaattta gtgttaccgc
      661 gactgttatt ggcttattta aagatggtgt taaagttaat gaaagtgcag aagatgtgag
      721 tgccatcttt gatgttaccc ccttttacag tgaaagcggc ggacaagtta gtgatagcgg
      781 ttttgtaagc aacaaagaac ttcgcgctgt cgtcagtgcg atgagtaaag caccacacgg
      841 tcaacattta catcatctaa cgcttgatta cggcgtaatt aaggttggtg ataaagtcca
      901 attagaaatc gacggggtag cgcgtcgctc cattatgctt aaccacagtg ccacacactt
      961 attacaccgt gcacttaacg atgtattagg tagtgaagta agtcaagctg ggagttatgt
     1021 tggtcctgat tatttacgct ttgactttaa ttataatacc cgtgtaactg acgaagaatt
     1081 agaaaaaatt gaattaattg ttaataaata tattagtgac tcagtcccat taactgttaa
     1141 atatatgact ttagcggaag cacaaaagct tggtgcaaaa gctttattta tggaaaagta
     1201 tggtgactta gttcgtgttg ttacttttgc cagaatatct gttggacttt gtggtggaac
     1261 acatattgat aatacaagta agattggtac ttttgtaatt gtaagtg
//
LOCUS       DAOSEX010000274         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_110126_length_1296_cov_3.290089, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000274 DAOSEX010000000
VERSION     DAOSEX010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110126_length_1296_cov_3.290089"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PK612_04645"
     CDS             <1..965
                     /locus_tag="PK612_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOH95427.1"
                     /translation="FIVLLTIGEIVPKTLAVKLNYQMSYAYANIFMFFYYLFTPLLFP
                     FRLISARRKKKNGNEEVNGDEIYTEDELQIIVDEIEEQGLIDEETSDLVRSALEFTET
                     EAYEIMTPRVDVFSYDIEDDIKELMKEEDIFKYSRIPVYEDTIDNIIGILRTKDLLKL
                     SLSDKKINVKSLIKPPLFVHHTKNVSQILTEFKQTKNHIAIVIDEYGGTEGIITMEDI
                     LEEIIGEIWDEADEVDEPIVKLEDNHYIIDGGLNIEDFFELFELSEDEDNDYDTVAGW
                     CIEMLDRFAKAGDEFTYEKITVKIIEVEGFVVEKVEVIVESEED"
     gene            965..>1296
                     /locus_tag="PK612_04650"
     CDS             965..>1296
                     /locus_tag="PK612_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH95428.1"
                     /translation="MYVEKFLERIPSLLNKTYVVTGANSGLGFALSTLLVSKGARVIM
                     ANRNPKRSEDAINKIRELYPAASVMVLPFDQSKKDSIEAFVNSLISHNIKPEGFVLNA
                     GIYSPAKDA"
BASE COUNT          428 a          180 c          270 g          418 t
ORIGIN      
        1 tatttattgt ccttttaacg attggtgaaa ttgtgccaaa aacactcgct gttaaattaa
       61 attatcaaat gtcgtatgct tacgcgaata tctttatgtt cttttattat ttatttacgc
      121 cgcttttatt tccgtttaga ctaattagtg cacgccggaa gaaaaagaat ggtaatgaag
      181 aagtaaatgg cgatgaaatt tatacggaag acgaattaca aattattgtc gatgaaattg
      241 aagagcaagg cttaattgac gaagagacaa gtgacttagt gcgcagtgcg cttgaattta
      301 cggaaacaga ggcttacgaa attatgacgc cgcgtgttga tgtctttagt tatgatattg
      361 aagatgacat taaagagtta atgaaagaag aagatatttt taaatatagt cgtatccccg
      421 tttacgaaga tacaatcgat aatattattg gtattttacg gacaaaagac ttacttaaac
      481 tttcactttc cgataagaaa attaatgtga aaagcttaat taaaccgcca cttttcgtcc
      541 atcatacgaa aaatgttagt caaattttaa ccgaattcaa gcaaacgaaa aaccatattg
      601 ccattgtcat tgatgagtat ggtggcaccg agggtattat tacaatggaa gacatccttg
      661 aagaaattat tggtgaaatt tgggatgagg ctgatgaagt tgatgagccg attgttaagc
      721 ttgaagataa tcattacatt attgatggtg gcttaaatat tgaagacttc tttgaattat
      781 ttgaattaag cgaagatgaa gataatgatt atgatacggt cgctggttgg tgtattgaaa
      841 tgctcgatcg ttttgctaag gcgggcgacg aattcactta tgaaaaaata actgtcaaaa
      901 tcattgaagt tgaaggcttt gttgttgaaa aagttgaagt tatcgttgaa agtgaagaag
      961 attaatgtac gttgaaaagt ttttagaacg aatcccgtct ttattaaata aaacttatgt
     1021 cgttactggc gctaatagtg gtttaggttt tgcccttagc acactccttg taagtaaagg
     1081 ggcacgcgtt attatggcta atcgtaatcc taaacggagt gaagacgcaa taaataagat
     1141 acgcgaatta tatcctgctg cgagtgtaat ggtactccct tttgatcaaa gtaaaaagga
     1201 ttctattgaa gcctttgtta atagtttaat tagtcacaat attaagcctg agggctttgt
     1261 gcttaatgct ggcatttatt cgcctgctaa agatgc
//
LOCUS       DAOSEX010000275         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_110648_length_1292_cov_3.426839, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000275 DAOSEX010000000
VERSION     DAOSEX010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110648_length_1292_cov_3.426839"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK612_04655"
     CDS             complement(<1..304)
                     /locus_tag="PK612_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HOH95429.1"
                     /translation="MNKEEIITLLAKENLSPQKRKGQNFLVDENVARKIVSFLEPNYI
                     DVIEVGPGLGALTKELLLKTVNFTAIEIDKGFVRVLRAKYPHLNIIEKDFLEYSVPR"
     gene            complement(297..836)
                     /locus_tag="PK612_04660"
     CDS             complement(297..836)
                     /locus_tag="PK612_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4093 domain-containing protein"
                     /protein_id="HOH95430.1"
                     /translation="MSSKTLLNCIFVAEGKRDEERLKKLDIPYIVRTDGLNVPRETTK
                     HLQELAKIHEVIILTDPDGPGQKIADKLLQEIPNAKVIKVIKKKAVKKGKVGIEHIPL
                     PLLAELIKPYKQETFTTSSDVTLIKLLDLGLSGPNSQFKKIKLATKYSLLVASLKNML
                     TQIQLLNISYKELKEALSE"
     gene            complement(833..>1292)
                     /locus_tag="PK612_04665"
     CDS             complement(833..>1292)
                     /locus_tag="PK612_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOH95431.1"
                     /translation="KERAIEHEFFIAQIELANKYKKPVLIHMRDATSDTYEVVKSNIP
                     RYQGIMHSYSGSKEMALRFIDLGMYISLAGPVTFLNAREPKEVAKVVSEERLLLETDA
                     PYLTPHPYRGKLNESANIPLIAKTIATIRNVSVEKVAEFTTKNAKTLFKL"
BASE COUNT          340 a          245 c          198 g          509 t
ORIGIN      
        1 cacgtggaac actatattct aagaaatctt tctcaatgat gttaaggtgt ggatactttg
       61 ctcttaatac tcgcacaaaa cctttatcaa tttcaattgc agtaaaattt acggtcttta
      121 aaagcagctc ttttgttagt gcacctagtc caggtccaac ttcaattaca tcaatgtaat
      181 taggttcaag aaagctgaca atcttacgcg ctacattttc atccactaaa aagttttgtc
      241 cctttctttt ttgtggcgac aagttttcct ttgctaataa tgttatgatt tcttctttat
      301 tcacttagtg cttcctttaa ttctttataa cttatattta atagttgtat ttgcgttaac
      361 atatttttaa gtgacgctac taataaacta tatttagtcg ctaactttat ttttttaaat
      421 tgtgaatttg gcccagataa ccctaaatct agtaatttta taagtgtaac atcggaagaa
      481 gtagtaaatg tttcttgctt ataaggttta attaattccg ctagaagcgg tagcggaata
      541 tgttctattc caacttttcc tttctttact gcttttttct ttattacttt tattacttta
      601 gcgttaggta tttcttgtaa aagtttgtct gcaatctttt gccctggtcc gtctggatct
      661 gttaagatta taacctcatg aattttcgct aattcttgta aatgctttgt tgtttcacgt
      721 ggaacattta gcccgtctgt ccttactatg taagggatgt ctaacttttt tagtctttct
      781 tcgtcgcgct ttccttccgc aacaaagata caatttaaca atgttttgct actcataatt
      841 taaataatgt tttagcgttc tttgttgtaa actctgcaac tttctcgaca gaaacattac
      901 gaatcgttgc tatggtctta gcaataagtg ggatatttgc actttcattt aatttgccgc
      961 gatatggatg cggggttaag taaggcgcat ctgtttcaag tagaagtctt tcttcgctta
     1021 caacctttgc cacttccttt ggctcacgcg catttaaaaa agttactggt cccgctagac
     1081 ttatatacat ccccaaatct ataaaacgca acgccatttc ctttgaacca ctgtaagaat
     1141 gcattattcc ttgatacctt gggatattac tctttaccac ttcatatgta tcacttgtag
     1201 cgtcgcgcat atgaataagc actggttttt tatatttatt tgcaagctca atttgcgcta
     1261 taaagaattc gtgttcaatt gctctttctt tt
//
LOCUS       DAOSEX010000276         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_112384_length_1279_cov_2.396242, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000276 DAOSEX010000000
VERSION     DAOSEX010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112384_length_1279_cov_2.396242"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="PK612_04670"
     CDS             complement(<1..958)
                     /locus_tag="PK612_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH95432.1"
                     /translation="MLFSPEIKESPTLLLGEKARQKKEKGEKVISLAVGEPDFPTPEY
                     IIDATKKALDEGYTKYSTPQGLLELRTLIAKEYKPYKAEEVIIFPGAKAAIHAALTSI
                     LEPGDEVIIVEPYYVSYPAMIKLAEPKAKIVKIVLNKDFTFPLEKLEEKITKKTKLLI
                     LNYPHNPTGQILSFQETMKIVNLVFRNKIYLLSDEIYDKLNFSGKAFHSFAEFPLIKD
                     QLVVINGFSKTYSMTGFRLGYALANQELIQKMNLVNQNIHTNTATFIQKGAVAALTNK
                     PMFLIPYNNELKERALYLNEEVNKLPLFSGVLPEGAFYYYMNI"
     gene            1062..>1279
                     /locus_tag="PK612_04675"
     CDS             1062..>1279
                     /locus_tag="PK612_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95433.1"
                     /translation="MKTFHFNDDYALINFDGINVNDALLILNSKSFEKVFRSFHRSLK
                     EKESVLLVSLGKLTATDLLEFYGLLFKY"
BASE COUNT          386 a          192 c          211 g          490 t
ORIGIN      
        1 ttatattcat atagtaatag aaagcacctt ctggtaatac accactaaag agtggtaatt
       61 tatttacttc ttcgtttaaa taaagtgctc tttcttttag ttcgttatta tacggaatta
      121 aaaacattgg tttattagtg agtgccgcta ctgcgccttt ttgaataaag gttgcggtat
      181 tagtgtgaat attttgattt actaaattca tcttttgaat taactcttga ttagcaagag
      241 cgtagcctaa tctaaaacct gtcatagaat atgttttact aaaaccattg atgaccacta
      301 gttggtcttt aataagcggg aattctgcaa aagagtggaa ggctttgccg ctgaaattaa
      361 gtttgtcata tatttcgtca cttagtaaat atatcttatt tctaaatact aaattaacaa
      421 ttttcattgt ttcttggaat gataagattt gaccagtggg gttatgcgga taattaagaa
      481 ttaataattt tgttttttta gtaatctttt cttctaactt ttctagtggg aaagtaaaat
      541 ctttattaag tactatttta acgatcttag ctttaggttc cgctagttta atcattgcgg
      601 gataactaac gtaataaggt tcaacgataa taacttcgtc acctggttca agaatactcg
      661 ttaatgccgc gtgaattgcc gctttagcgc cgggaaagat aattacttct tcagctttgt
      721 aaggtttata ttctttagca attaaagttc gtaattctaa taacccttgc ggagtggaat
      781 atttcgtata tccttcatca agtgctttct ttgttgcgtc aattatatat tcaggcgtag
      841 gaaaatcagg ttcgcctacc gctagtgaaa taactttttc acctttttct tttttttgcc
      901 gcgctttttc accaagtaaa agtgtcggac tttcttttat ttccggacta aataacataa
      961 aaaatacctc ttttcttatc aattttacca tattttgctc attttttgat aaaaaatggt
     1021 cgcttatggt acattatata attaagagaa ggggaatatt gatgaagact tttcacttta
     1081 atgatgacta tgcactaatt aactttgatg gtattaatgt taatgatgcg ctcttaatat
     1141 taaactccaa aagttttgaa aaagtattta gaagctttca ccgctcttta aaagaaaaag
     1201 agagtgtttt acttgttagt ttaggtaaac taaccgcgac tgatttatta gagttctatg
     1261 gccttctttt taaatatga
//
LOCUS       DAOSEX010000277         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_113103_length_1274_cov_1.824446, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000277 DAOSEX010000000
VERSION     DAOSEX010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113103_length_1274_cov_1.824446"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK612_04680"
     CDS             complement(<1..188)
                     /locus_tag="PK612_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95434.1"
                     /translation="MERNKTLFKILAILLLIVGIILIVLGLINIVEVTTYNKVLLPGE
                     EPKSTNLLFPLLATPCMFG"
     gene            complement(175..522)
                     /locus_tag="PK612_04685"
     CDS             complement(175..522)
                     /locus_tag="PK612_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOH95435.1"
                     /translation="MSDDVIKKILKFRDDRDWKQFHNGKDLAISLNLEASELLELFQW
                     SGTDLTASEKVEAMKEELADILMYAILFADRYNIDLNEALEKKLEQNNNKYPASIAKG
                     KKDKYTELKNGKK"
     gene            complement(525..1067)
                     /locus_tag="PK612_04690"
     CDS             complement(525..1067)
                     /locus_tag="PK612_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOH95436.1"
                     /translation="MPDKLLKDYFVILVLDDITSLKVRTLKLTDKDFKVPYRNFYPHI
                     NLGRFLQADKHTVVKAMKKVSKLINPFELDINNVEMLTEYLMVLTFAKNEKLDFVHRK
                     LHELVPYKSDDWTNPDSGKYVPHISIYYNPGEDVREMHKHYLNIFKPFKGKVVAIELS
                     EYDGQSFRVSHRYELKKRLF"
BASE COUNT          404 a          202 c          171 g          497 t
ORIGIN      
        1 ccaaacatac atggtgtcgc taataaagga aaaagaaggt ttgttgattt aggttcctct
       61 cctggtaata acactttgtt gtatgttgtt acttccacaa tattaataag acccaataca
      121 attaaaataa ttcccactat taggagtaat attgctaata ttttaaaaag tgttttattt
      181 ctttccattt ttaagttctg tatatttatc tttcttaccc ttagcaatgc ttgctgggta
      241 tttattatta ttttgttcta gtttcttttc taaggcttcg tttagatcaa tattgtatct
      301 atccgcaaaa agaatagcat acattaatat atctgcgagt tcttccttca ttgcttcaac
      361 tttttcactt gcggttaagt ctgttcctga ccattgaaaa agttctaaaa gttcacttgc
      421 ttcaagattt aacgaaatag ctaaatcttt accattatga aattgtttcc aatcacggtc
      481 atctctaaat tttagtattt ttttaatgac atcatcacta atcattaaaa taatctcttt
      541 tttaattcgt atcggtgtga tacgcggaaa ctttgtccgt cgtattcact tagttcaata
      601 gcgactactt tgcctttaaa cggtttaaag atatttaagt aatgtttatg catttcacgg
      661 acatcttcac caggattata ataaatagaa atatgtggga catactttcc tgaatctgga
      721 ttcgtccagt catcagattt atagggcact agttcatgaa gtttacgatg aacaaaatct
      781 aatttttcgt ttttcgcaaa ggttaatacc atgagatatt ccgttaacat ttcaacatta
      841 ttaatatcta attcaaaagg gttaattaat ttagatactt tcttcattgc tttaaccaca
      901 gtatgtttat cagcttgtaa aaatcttcct aagttaatat gtggataaaa attacgataa
      961 ggcactttga aatctttgtc agttaatttt aaagttctta cttttaaaga agtaatatca
     1021 tcaagcacta atattacaaa gtaatctttt aataatttat ccggcataaa agtcctccta
     1081 aaaagttgga ttcatagaca ttatactata aagtaaaaga atattatatt tttaagggtc
     1141 ttctttattt ttataaaata aaaagtgtta ctaacactag tcccaagtta tcacttggtt
     1201 aggagtatat gatttacgat tcttttgtat ttcgcgtcgt tttgccttaa aatcttcttt
     1261 aataattccc atcg
//
LOCUS       DAOSEX010000278         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_113683_length_1269_cov_3.908567, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000278 DAOSEX010000000
VERSION     DAOSEX010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113683_length_1269_cov_3.908567"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1146
                     /locus_tag="PK612_04695"
     CDS             43..1146
                     /locus_tag="PK612_04695"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOH95437.1"
                     /translation="MTNYLSKETANSGRGDYQNAYLVDKRVDETRALIHKFINSKHVQ
                     EVVFTQGTTASLNMVAKCYGEKFLKEGDEILLSIAEHASNTLPWMQVAKKTGAKIVYM
                     PLSEDGKVTPEIVEKYMTEHTKVVSVLHVSNVLGHVHGIKQIAKIVHDKGAIIVLDGA
                     QSVPHMKVDVQDLDVDFLAFSGHKMLAPTGIGVLYGKKELLEKMDPYQTGGGMNFSYD
                     KEGNYELFKVPLKFEAGTLNIEAIYGLHAAIEYINKIGIENISKHEHKLRTYAVSKLK
                     EMDHIILYNENAETGIITFNVKNVFAQDVATYFNSKNIAVRSGEHCAKLLKEHLREVA
                     TVRASLYLYTTKEEIDAFVEAARTAENYLDAYF"
BASE COUNT          445 a          185 c          258 g          381 t
ORIGIN      
        1 aacgctgcta caacatataa accataagtt gtaatcgatg cagtgactaa ttatttatca
       61 aaagaaaccg ctaatagtgg gcgcggcgat tatcaaaatg cttatttagt cgataaaaga
      121 gttgatgaaa cgcgcgcttt aattcataaa tttattaata gtaaacatgt gcaagaagtt
      181 gtttttaccc aagggacaac ggcctcatta aatatggttg ctaagtgtta tggtgagaag
      241 ttcttaaaag aaggtgacga aatattatta tcaattgctg aacacgcatc taacacctta
      301 ccctggatgc aagtggccaa aaaaactggt gctaaaatcg tctatatgcc acttagtgaa
      361 gacgggaaag tcactccaga aattgtcgaa aaatatatga cagaacatac aaaagttgtc
      421 agtgtacttc atgttagcaa tgttttaggt catgttcatg gtattaaaca aattgcgaaa
      481 atagtgcatg ataagggcgc tattattgtt ttagatggtg cgcaaagcgt cccgcatatg
      541 aaagttgatg ttcaagactt agatgttgac tttttagcgt ttagcggaca taaaatgtta
      601 gcgccaacag gtatcggtgt actttatggt aaaaaagaat tattagaaaa gatggatcct
      661 taccaaacgg gcgggggcat gaattttagt tatgataaag aagggaatta tgaattattt
      721 aaagtcccac ttaagtttga agcaggcaca ttaaacatcg aggcgattta tggcttacat
      781 gccgcgattg aatatattaa taagattggt attgaaaata ttagtaaaca tgaacataag
      841 ttaagaacat atgctgtttc taaattaaaa gaaatggatc atattatttt atataacgaa
      901 aatgctgaaa ctggtattat tactttcaat gttaaaaatg tgtttgcaca agatgttgct
      961 acatatttta atagtaagaa tattgccgtt cgtagtggag aacattgcgc taaattgctg
     1021 aaagaacatt tacgcgaagt ggcaactgta cgtgcaagtt tatatttgta tacgacaaaa
     1081 gaagaaattg atgcctttgt tgaggcagct agaacagcgg agaattattt agatgcctat
     1141 ttttaataac gagcttatgc gggcgacaat tttaacgcat tatgaaaacc cttatcacaa
     1201 ggaaaccccg caaaatgcgg atgaatttat gcaaattcac cttacttcca gtggttgtat
     1261 agataattt
//
LOCUS       DAOSEX010000279         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_114387_length_1264_cov_3.740281, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000279 DAOSEX010000000
VERSION     DAOSEX010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114387_length_1264_cov_3.740281"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PK612_04700"
     CDS             complement(<1..210)
                     /locus_tag="PK612_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOH95438.1"
                     /translation="MKLYSNEIVFRGHPDKVCDQISSAILDACLRQDPRTRAGIEVVG
                     GKGKIFITGEITTKAKINAKAITKRV"
     gene            complement(311..496)
                     /locus_tag="PK612_04705"
     CDS             complement(311..496)
                     /locus_tag="PK612_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95439.1"
                     /translation="MTDAKNFKIIFYDKDDCEHLVKYGAATSCEFIEECIEMFKKKYD
                     HFKSAVVIFNGEIIEYK"
     gene            complement(489..980)
                     /locus_tag="PK612_04710"
     CDS             complement(489..980)
                     /locus_tag="PK612_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95440.1"
                     /translation="MTIRNHKAREIVGLLRGDSQYVISEKPLEIGESYHIYLRPLKTE
                     SLTRVKRLGFVLQSRKHPLGKPIQDCIIGWYEINKSIALETKEIVDLLKTNNLSSITV
                     KIDNQPITLYGDDLPKKETFYLNLVSELRIYSHPMKLSDYQNRYGKELKSAKDLQEVK
                     LYD"
     gene            complement(993..>1264)
                     /locus_tag="PK612_04715"
     CDS             complement(993..>1264)
                     /locus_tag="PK612_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95441.1"
                     /translation="PHLVTASGIMFKTYTDLNAYQLLTVEQDLINMLNNLNDNLSANG
                     VVLIKWNNKDFSLKPLFKKSKLKLLFGTTSYKKTETYYYVEIKGI"
BASE COUNT          328 a          228 c          194 g          514 t
ORIGIN      
        1 aacacgcttt gtgattgctt tggcgttgat ttttgcttta gtagttattt ctccggtgat
       61 aaatatttta cctttgcctc cgacaacttc aattccggct ctggtgcgag ggtcttgtct
      121 taaacaagca tctaatatcg cactactgat ttggtcacat actttatctg ggtgccccct
      181 gaacacgatt tcatttgaat ataatttcat tttttaagcc ccttttattt attttaacat
      241 ttttttaatt tttcttccaa tctatctttt tatatattgt ttcacagaca agttgatttc
      301 atgtgattca ttatttgtat tcgataatct cgccattaaa tattactacc gctgacttga
      361 agtgatcata tttctttttg aacatttcga tacactcctc gatgaattcg cacgatgttg
      421 ccgccccata tttaacgaga tgttcgcagt cgtctttgtc atagaatata attttaaagt
      481 tcttggcatc agtcatataa ttttacctcc tgtaaatctt tggctgattt tagctctttc
      541 ccatatcggt tttgataatc gcttaatttc atcggatgtg agtagattcg tagttcactg
      601 actaaattaa gatagaatgt ttccttttta ggtaagtcat cgccataaag tgtaatgggc
      661 tgattatcga ttttaaccgt tatgctcgat aagttgtttg tttttaataa gtcgacaatc
      721 tcttttgttt caagcgcgat cgacttgttg atttcatacc agccgataat gcagtcttga
      781 atcggttttc ctaacggatg ttttcgactt tgcagaacga atcctaaccg cttaactctt
      841 gttaaacttt cggtcttgag tggtcttaaa taaatgtgat aactttcgcc aatttcaagt
      901 ggcttctcac tgataacata ttgtgaatcg ccccttaata aaccgacaat ttcccttgcc
      961 ttgtgatttc taattgtcat actatctcct ttctatatac ctttaatttc tacataataa
     1021 taagtttcgg tttttttata acttgttgtt ccgaataata gttttaattt tgattttttg
     1081 aataatggtt tgagtgaaaa atctttatta ttccatttta ttaaaaccac tccgtttgct
     1141 gataagttat catttaaatt atttaacata tttattaaat cttgttctac tgtcaataat
     1201 tggtaagcgt ttaaatcggt gtaagtttta aacataatgc cacttgcggt tactaaatgc
     1261 ggcg
//
LOCUS       DAOSEX010000280         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_114796_length_1262_cov_1.784590, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000280 DAOSEX010000000
VERSION     DAOSEX010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114796_length_1262_cov_1.784590"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PK612_04720"
     CDS             complement(<1..936)
                     /locus_tag="PK612_04720"
                     /inference="COORDINATES: protein motif:HMM:NF023156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HOH95442.1"
                     /translation="MEKRLRVALYLKMLVGFMISFIIAYLLKMPTSYTAGVIAVLNLW
                     YSRDNVVKTALIRVLSSLIGLATSALLFFLLGYNIFTLFFMVFIVLALLYVFKLEYGA
                     TPTLVLIGQQWAQQTSEAPLNAFVTLLIGVIPALLLNLITFRKSKILLINQQKLDTEI
                     ANIFTYFEKDEIYDFSVIDKILKDTRESLQIALDNYEVKNIMQNFHYINMRTEQINVL
                     KRIVNDLNMLEASPYKKEITNYLIKFKDRIGLEDYASDLLVKHDKLVAYYRALELPKT
                     RDEFEHRAILFAILEKIKIFLILKLDYHKIYPCQKQ"
BASE COUNT          457 a          175 c          213 g          417 t
ORIGIN      
        1 ttgcttttga cacggataaa ttttgtgata atcaagttta agaattaaga agattttaat
       61 tttttctaat atcgcaaata atatagcgcg gtgttcaaat tcgtctctag tttttggtag
      121 ttctagtgcg cggtaatagg ccactagttt atcgtgctta acgagtaaat cacttgcata
      181 atcttcaagt ccaatgcggt ctttgaactt aattaaataa ttagtgattt cttttttata
      241 tggtgaagct tctaacatat ttaaatcgtt tactattcgc tttaatacat taatttgttc
      301 ggtgcgcata ttaatataat ggaagttttg catgatgttt tttacttcat aattatctag
      361 cgctatttgc agactttcac gtgtatcttt gagtatttta tcaatcacgc tgaagtcata
      421 aatttcatct ttttcaaagt aggtaaatat atttgcaatt tctgtatcta gtttttgctg
      481 attaataagt aagatctttg atttacggaa agtaattaag tttaaaagta aagcagggat
      541 aacaccgatt aaaagcgtga caaaggcatt taatggcgct tctgaagttt gttgcgccca
      601 ttgttgccca attaagacaa gggttggggt tgcgccgtat tcaagtttaa aaacatagag
      661 gagcgctaaa acgatgaata ccataaagaa gagcgtaaaa atgttatagc ctaataaaaa
      721 gaagagcagt gcactagtgg caagcccaat taaacttgat aatacgcgaa taagggcagt
      781 tttgacaaca ttatcacggc tataccataa gtttaaaaca gcaataacac cagcagtgta
      841 gcttgtcggc atcttaagta aataggcaat gataaaacta atcataaagc ccactagcat
      901 tttaagataa agcgcaactc gtaatctttt ttccatacat tacatataat aacaaaaagt
      961 ttagtatagt aactctttaa agcattagta agataatagt ttgtaagcaa tatataactt
     1021 aaaaagtaat aaaattatgt tgatttgctc cataaagtgt tatttaaggc aaggaagtgg
     1081 agtaaaaaaa catttgccga cacttgatgt aaagaagaga caaagaatct tttatataat
     1141 agaaagcaag tattaatgaa cataatttga cgatatttac taaataacga acataatatc
     1201 aaaataatgt tgtatcatta aaatgtatat actcacataa ataagaaagg aatcatatat
     1261 gc
//
LOCUS       DAOSEX010000281         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_114865_length_1261_cov_2.912935, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000281 DAOSEX010000000
VERSION     DAOSEX010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114865_length_1261_cov_2.912935"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PK612_04725"
     CDS             complement(<1..188)
                     /locus_tag="PK612_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOH95443.1"
                     /translation="MVKNKVAVIIDTDLGADCDDVGTLVLALQLEKHGFCDIKAVGYC
                     ATNIYGVQCVDIVRSFYGG"
     gene            complement(175..879)
                     /locus_tag="PK612_04730"
     CDS             complement(175..879)
                     /locus_tag="PK612_04730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOH95444.1"
                     /translation="MFPNNTHKAITFSFDDGVTQDRRLVALFNKYNLKCTFNLNSGGF
                     GKDGVCHWQKWSAPHNKVNSAEVKALYSGHEVAVHTVNHPDLTTIADEKIIIDEIEQD
                     RKVLEKLTGDKIVGMAYPFGTLNDRIVNIIKRNTPIKYARSIWATYNFDLQDNLLAFR
                     PTIHFLDFKKMYELAGTFLNTEYNEDKIFYIWGHSYELDFEDAWEEFEKFLAYIANKK
                     DVFYGTNKEVLLNGQK"
     gene            complement(881..>1261)
                     /locus_tag="PK612_04735"
     CDS             complement(881..>1261)
                     /locus_tag="PK612_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95445.1"
                     /translation="TYQFQDKNNYYEFMYSNDKYLKLSGNSTYRLTLKYKLMTEIRLH
                     SDPSLKGYAYIFARSKATHVDGNPDNPLLVGTEIHFASASTLETVYTETFELMTPSCD
                     DTMIIIGLYGIGTMIVDDILVEKD"
BASE COUNT          393 a          269 c          182 g          417 t
ORIGIN      
        1 ccgccataga aagaacgaac aatatcaaca cattgcaccc cataaatatt cgtcgcacaa
       61 taaccgactg ccttaatatc gcaaaaccca tgcttttcta attgcaacgc taacacaagg
      121 gtcccaacat catcacaatc agcgcctaaa tcagtatcaa taatgacagc aactttattt
      181 ttgaccattt agtaacacct ctttgtttgt cccgtaaaaa acatcttttt tattagcaat
      241 atacgctagg aatttttcaa attcttccca cgcatcttca aaatccaatt cgtaagaatg
      301 cccccaaata taaaatatct tatcctcatt atattcagtg ttaagaaaag tgcctgctag
      361 ttcatacatc tttttaaaat ctaaaaaatg aattgttggc ctaaacgcta ataagttatc
      421 ttgtaaatca aaattgtatg tcgcccaaat acttcttgcg tatttaatcg gcgtatttct
      481 tttaataata ttaactatcc tatcatttaa tgtgccaaaa ggatacgcca tcccaactat
      541 tttgtcgcct gttaattttt ccaacacctt tcggtcttgt tcaatctcat caatgatgat
      601 tttttcgtca gcgattgttg ttaaatcagg atggttaaca gtgtgaaccg ctacttcgtg
      661 gccgctatat aaagccttaa cctctgcgct atttaccttg ttatgtggtg cactccattt
      721 ttgccagtga cagacaccat ctttcccaaa accaccacta tttagattga aagtgcattt
      781 taaattatac ttattaaata atgcaacgag gcggcgatct tgtgttaccc catcatcaaa
      841 ggaaaaagtt atcgctttat gtgtattatt tggaaacatc tcaatccttc tccactaaaa
      901 tatcatcaac aatcattgtc ccaatgccgt ataagccaat aataatcatt gtgtcatcgc
      961 aacttggcgt cattagctca aaggtctcgg tataaactgt ttctaaagta ctggcgctgg
     1021 caaaatgaat ttctgttccc acaagtaacg gattatcagg attaccgtcg acatgagtag
     1081 ccttgcttcg tgcaaagata taggcatagc cctttagtga aggatcacta tgtagcctaa
     1141 tctccgtcat caatttatat tttaaagtta aacgataagt tgaatttccg ctaagtttta
     1201 aatacttgtc atttgagtac atgaattcat agtaattatt cttatcttgg aattgatatg
     1261 t
//
LOCUS       DAOSEX010000282         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_115799_length_1255_cov_2.265833, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000282 DAOSEX010000000
VERSION     DAOSEX010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115799_length_1255_cov_2.265833"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1099)
                     /gene="recA"
                     /locus_tag="PK612_04740"
     CDS             complement(98..1099)
                     /gene="recA"
                     /locus_tag="PK612_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOH95446.1"
                     /translation="MSKKKDANILQETLKEIEKSFGKGAIMKLGERPVINVDVIPSGS
                     LLIDEALGVGGYPKGRIIEIFGPESSGKTTLALHAIAECQKKGGQAAFVDAEHAIDPI
                     YAKNLGINIDELLLAQPDSGEQALEIVVRLAESGVIDLIVVDSVAALVPEAELSGEMG
                     DNQVGLQARLMSKAMRKLAGVLNKAECTVIFINQLREKVGIIYGNPEVTSGGRALKFY
                     SSIRIDIRRGEAIKQGSKLIGNQAKIRIVKNKVAPPFAACGIDIIYGKGISKIGEILE
                     LAVNRGFIKKSGSWYEHDGLKIGQGREAAQNYILERPELLADLEEKIKTGVVATETK"
BASE COUNT          378 a          263 c          187 g          427 t
ORIGIN      
        1 acaacttttt agcattaatt aaagcaaaga tgattagtaa agtacacaat tttaaaaatt
       61 gtgtttttta attttaaaat ttataaaata attgtaatta tttagtttca gtggcgacta
      121 cgcctgtttt aattttttct tctaaatcag cgagtaattc tgggcgctcc aaaatataat
      181 tttgcgctgc ttcgcggcct tgtccaatct taagtccatc gtgttcatac cacgaacccg
      241 actttttaat aaatccgcgg ttaaccgcta attcaagaat ttccccaatt ttagaaatac
      301 ctttgccata aattatatcg attccacaag cggcaaatgg tggtgcaact ttgtttttta
      361 caatacgaat ttttgcttga ttaccaatta atttagaacc ttgcttaatc gcttcaccgc
      421 gtctaatgtc aatacgaatg gaagaataaa attttaatgc acgaccacca gaagtaactt
      481 caggattgcc ataaattatt ccaacctttt cgcgtagttg attaataaag ataacagtac
      541 actcagcttt atttaataca cccgctagtt tacgcattgc ttttgacatc aggcgtgctt
      601 gtagtcctac ttggttatca cccatttcgc cagatagttc tgcttcaggt acaagtgcag
      661 ccacactatc aacaacgatt aaatcaatca caccactttc ggcaagacgg acaacaattt
      721 ccagtgcttg ttcacctgaa tcaggttgtg ctaaaagtaa ttcatcaata ttaattccta
      781 aatttttagc ataaatcggg tcaattgcat gttcagcatc gacaaaagca gcttgaccac
      841 ctttcttttg acattcagca attgcgtgca gcgctaaagt agttttccca ctactttctg
      901 gtccaaagat ttcaataata cgcccttttg gatagccacc aacgccaagt gcttcatcaa
      961 ttaataatga gccgctcgga atgacatcaa cattaataac tgggcgttcg cctagtttca
     1021 taatggcgcc tttaccaaac gacttttcaa tttcttttaa tgtttcttgt aaaatatttg
     1081 catctttctt ttttgacata ataattttct ccttatatag atatatacaa tattattcga
     1141 agttttttaa cactctcgct aacattttta aggcagcatt cactgcatca gtgcggactt
     1201 taagccgatc acctttaaat ttgaagtgtt caacaatttc gtcgttagca aacgc
//
LOCUS       DAOSEX010000283         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_117574_length_1243_cov_2.301347, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000283 DAOSEX010000000
VERSION     DAOSEX010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117574_length_1243_cov_2.301347"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /gene="ybeY"
                     /locus_tag="PK612_04745"
     CDS             complement(<1..461)
                     /gene="ybeY"
                     /locus_tag="PK612_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOH95447.1"
                     /translation="MLKLTVEFYDDYAPTYKNYRAIFKRIYAKTMKLLDYMTGEFLVE
                     VTITDEERIRALNKEYRAKDAATDVLSFAFNDIKPGHKFKEPFVHLGSIIICVPVAQR
                     QALELGHDARREFKFLFTHGLLHLLGYDHTNEKEEQVMITLQDQIIGKRGG"
     gene            634..>1243
                     /locus_tag="PK612_04750"
     CDS             634..>1243
                     /locus_tag="PK612_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOH95448.1"
                     /translation="MKSDFTFIYSAPKVTDEKLPAVFLFHGYRSDEKDLLSLVASLES
                     THHIFSVRGPLSFMFSGYAFWDMQDLENPNLEQYQSAVESVQNFIVNAITQYNLEKKD
                     ITLLGFSQGAVLVQSIALRAQKLVNKVVALSGHLPYFAKDEEYATLNLNKLHIFISNG
                     INDNVVPYAWGKESADYFKKRGADVTFKSYFSGHYVSRDNARD"
BASE COUNT          411 a          216 c          210 g          406 t
ORIGIN      
        1 ttccctcctc ttttaccaat aatttggtct tgcaaagtga tcatcacctg ctcttctttt
       61 tcatttgtgt gatcataccc taataaatgt aagagtccgt gcgtaaataa gaatttaaat
      121 tcacgccggg catcgtgtcc taattcaagc gcttgacgtt gcgctactgg gacacaaata
      181 ataatggaac ctaaatgtac aaagggttct ttaaacttat gaccaggttt aatatcatta
      241 aaggcaaatg ataatacatc agttgctgca tctttagcgc gatattcttt atttaatgcc
      301 ctaatccttt cttcatcagt aattgtaact tcaacaagga attcacctgt catataatca
      361 agtaatttca ttgtttttgc ataaattcgt ttaaaaatag cccgatagtt tttataagtc
      421 ggtgcataat catcataaaa ttcaacagta agtttaagca tcaatataat tttaccatag
      481 attgattaaa ttaacgcagt gctttaatcg agtaataaag taaaccacta aagtggtatt
      541 tcaaatatac atagtacaat gttaagataa tgtatataaa attcttattt gttacattct
      601 taaaaacaaa gcattaaaag aaaggaaata gcaatgaaat ctgactttac ctttatatat
      661 tccgcaccaa aagttacaga tgaaaaatta ccagcggttt ttttatttca cggctatcga
      721 agcgatgaaa aagatttact ttcacttgtt gcaagtttag aaagcaccca tcatattttt
      781 agtgtgcgcg gaccgctcag tttcatgttt agtggatacg cattttggga catgcaagac
      841 ttagaaaacc ctaatcttga acaatatcaa agcgcagtgg aaagtgtaca aaattttata
      901 gttaatgcta tcactcaata taatttagaa aagaaagaca tcacgctttt aggttttagt
      961 caaggtgcag tacttgtgca atcaattgcg ctcagggcac aaaaactagt gaataaagtc
     1021 gtagcactaa gtggtcatct tccttatttt gctaaggatg aagaatatgc gacactaaat
     1081 cttaacaaac tacatatttt tatctctaac ggcattaatg ataatgttgt gccttatgct
     1141 tggggtaagg aaagcgcgga ctattttaag aagcgcggcg ctgatgtgac ttttaagtct
     1201 tattttagtg gccactatgt ttcgcgtgac aacgcccgcg att
//
LOCUS       DAOSEX010000284         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_118517_length_1236_cov_3.836579, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000284 DAOSEX010000000
VERSION     DAOSEX010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118517_length_1236_cov_3.836579"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PK612_04755"
     CDS             complement(<1..470)
                     /locus_tag="PK612_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95449.1"
                     /translation="MKKTFTLMTLALGATLVLGGCTKPNTEATAVIKSQTMAFEATTA
                     LTLVGGFETVPEVKALQKSLAAEGVAEPDIPVATLDILFNNGVDFQINREDSDLAGYT
                     YVDTISFNIGTDSRIEYKLYYNLETAEEEIDDDSDDDELLNNEVASKARYGENK"
     gene            complement(561..>1236)
                     /locus_tag="PK612_04760"
     CDS             complement(561..>1236)
                     /locus_tag="PK612_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95450.1"
                     /translation="PEGSIPGGMGLIKPWYSISLSDPEKLELEAALDETLPLVSDFFS
                     LDKGFNYQKNKGNYAGTYGTYTHEFIINEYTRIIGNVEIEEDDTETETELEGEIIIHD
                     THFHFEGETEIDTADNETDISLKIEYNDDSYLEIESENEADEQTFKYKLYLDGDEKLN
                     VEIKSYNTHGNGNGGRCAEVKTKYNNEDYEFYITKKSGNYVIEYLEMIIIATETEDKK
                     FIYSYE"
BASE COUNT          333 a          233 c          192 g          478 t
ORIGIN      
        1 tgtttgtttt cgccgtagcg tgctttactt gcgacttcat tatttaataa ttcgtcatcg
       61 tcactatcat catcaatttc ttcttccgct gtttctaaat tgtaatagag tttgtattca
      121 atgcggctat ctgttccgat gttgaaggat atcgtatcaa cataggtata acctgctaaa
      181 tcactatctt cacgattaat ttggaagtca acaccattat tgaataagat gtcaagtgtt
      241 gcaactggga tatcaggttc ggcaacgcct tccgcggcaa gtgatttttg taaggctttc
      301 acctctggta cagtttcgaa tccgcccact agtgtgagag cggtcgtggc ttcaaaggcc
      361 attgtttgac ttttaattac ggctgtggct tcagtatttg gttttgtaca gccaccaagc
      421 acgagtgttg ctccaagagc gagagtcatg agtgtaaacg tttttttcat aagtagttcc
      481 tccttttttc cgttcacaat aaatacgaat taaaattctt ttttattgga attatttaaa
      541 aattattttt attttatttt ttattcgtaa gaataaataa attttttatc ttcagtttct
      601 gttgcaataa taatcatttc aagatattca atgacataat taccgctctt ttttgtgatg
      661 taaaattcat aatcttcatt gttatatttc gtttttactt ctgcgcaacg accaccatta
      721 ccattaccat gcgtgttata acttttaatt tcaacattaa gtttttcgtc gccatcaaga
      781 tatagtttat atttaaatgt ttgttcatca gcttcgtttt cgctctcgat ttctaaatat
      841 gaatcatcat tatattcaat ctttaaacta atatctgttt cgttatcagc agtatcaatt
      901 tctgtttcgc cttcaaaatg gaaatgtgtg tcgtgaataa tgatttcacc ttcaagttcc
      961 gtttccgttt cggtatcatc ttcttcaatt tcaacattac caataatgcg tgtgtattca
     1021 ttaataataa attcatgcgt gtatgtacca tatgttcccg cataattccc tttattcttc
     1081 tgataattaa aacctttatc aagactaaag aagtcactca ctaagggtag cgtttcatct
     1141 agcgctgctt ctagttctaa tttttcagga tcactaagtg agatactata ccatggttta
     1201 attaatccca ttccaccagg aattgagcct tcaggg
//
LOCUS       DAOSEX010000285         1234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_118892_length_1234_cov_2.586090, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000285 DAOSEX010000000
VERSION     DAOSEX010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118892_length_1234_cov_2.586090"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="PK612_04765"
     CDS             complement(<1..879)
                     /locus_tag="PK612_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95451.1"
                     /translation="MRKQIIHNIFKKALVRDIYVTNNEVSGAFIRVYYDELYIFDGFG
                     HLLYQGTQEIHSISSGVNNEGKHYVKLYINPVFTTPITYDEVYFYVYDALGNMTRVAT
                     DSAPNTQKESSNAEDLSEYGLPLYSLVTNEESQEIKDATGKTIVTLKKEVGVYRSQIF
                     AGHYFYQKITKGSKSAINYDLKDIYGNTYKVSTHSVNLKTGRIKTLKFPYFLAAISPL
                     YDKNNIYNYAVAEIATFDAFNSKTANYVSEKFIINYKGHLLDQVSDMPLENFVEIQGG
                     FYNKATNKLYDEDVKLI"
     gene            complement(939..1157)
                     /locus_tag="PK612_04770"
     CDS             complement(939..1157)
                     /locus_tag="PK612_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95452.1"
                     /translation="MKKHNFFIITLTVVTLLSGCKKYRSRGSVLSGELLRNNITKVLK
                     KAEPLNVKKIYEQLYLVMMKFITIMTLH"
BASE COUNT          400 a          225 c          174 g          435 t
ORIGIN      
        1 aattaattta acgtcttcat cataaagttt attcgtagct ttattataaa atccgccctg
       61 aatttccaca aaattttcaa gcggcatatc acttacttga tcaagcaagt gacctttata
      121 attaataata aatttctcgg aaacataatt tgctgttttg ctattaaaag catcaaaagt
      181 agcaatctca gcaacagcat aattataaat gttattttta tcatagagtg gacttatcgc
      241 ggctaagaag taggggaact taagcgtttt aatgcgacct gtttttaaat taacactatg
      301 tgttgacact ttataagtgt tgccataaat atcttttaaa tcgtaattaa tcgcactttt
      361 gcttcctttt gtgatctttt gatagaaata atgacccgca aagatttggc tacgataaac
      421 gcctacttct ttttttagcg tcactatcgt tttaccagta gcgtccttaa tttcttgact
      481 ctcttcattt gtaactagcg agtaaagagg taatccgtac tctgacaaat cttccgcgtt
      541 actactttct ttttgtgtat ttggcgctga atcagtcgca actcttgtca tattaccaag
      601 ggcgtcatac acataaaaat aaacttcgtc ataagtaatt ggagtagtga atacgggatt
      661 aatatatagt tttacataat gttttccttc attatttacc cctgaactta tactatgaat
      721 ctcttgtgtg ccttgataga gtaaatgacc aaagccatca aaaatataga gttcatcgta
      781 gtacacacga ataaaggcgc ctgatacttc attattagta acataaatat cacgcactaa
      841 ggctttttta aatatattat gaataatttg tttacgcatt gctaatgaat aaaacccaat
      901 acaatcatca ctatttttcg tcgcaattat atctctaatt agtgcagtgt cataatggtg
      961 ataaatttca tcatgactaa atacaattgt tcatatattt ttttaacatt taacggttct
     1021 gcttttttta gcacttttgt aatgttgtta cgcagtaact ccccgcttaa cacactacca
     1081 cgcgacctat acttcttaca tcctgaaagt aaagtcacaa cggttaaagt aataataaaa
     1141 aaattatgtt ttttcactaa gttcctcctt taccaatatc gatattagaa attaggccgc
     1201 aaagacataa gtgagcacta atccagaaac aatt
//
LOCUS       DAOSEX010000286         1233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_119092_length_1233_cov_2.123939, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000286 DAOSEX010000000
VERSION     DAOSEX010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119092_length_1233_cov_2.123939"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PK612_04775"
     CDS             <1..776
                     /locus_tag="PK612_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608947.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HOH95453.1"
                     /translation="LGLLHMDVIQERLEREHDLNLILTAPSVIYHVYLTNGTMLKIDN
                     PSKLPDFTLVDRIEEPYVKAQIMTPQEYVGPIMELCQDKRGIYVDLSYLDDTRMQVTY
                     EIPLSEVIYNFFDKLKSYSKGYATLDYSLSGYKASNLAKMDILLNGQPVDALSLIVYR
                     PTAYNRGVAMCRALKNIIPRQQFEVPIQAAINNKIIARTDIKAVRKDVLAKCYGGDIS
                     RKRKLLEKQKEGKKRMKLVGSVEIPQEAFMSVLSIDDDN"
     gene            855..>1233
                     /locus_tag="PK612_04780"
     CDS             855..>1233
                     /locus_tag="PK612_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95454.1"
                     /translation="MSTNQGERFSNERYLTMNQVSRALGTTLIDDIWKEVLQYRNMFS
                     VTIPLRTVERNRLRVVLTPKINDRLNQLDRRISRIQARIQEVYVDANHQKALKTLLTR
                     DVLKTIAASYEINFHDGIVNQLIN"
BASE COUNT          421 a          206 c          234 g          372 t
ORIGIN      
        1 ttttagggct tttacatatg gatgtaattc aagaacggct tgaacgggaa cacgacttaa
       61 atttaatctt aacagcgcca agtgttattt atcatgttta tctcactaat gggacgatgt
      121 taaaaataga taacccaagt aaattaccag attttacact tgttgaccgt atcgaagaac
      181 catatgttaa agcacaaatt atgacaccac aagaatacgt cggtcccatt atggaattat
      241 gccaagataa gcgcggtatt tatgtcgatt tatcttatct tgatgatacg cgaatgcaag
      301 ttacttatga gattccctta tctgaagtaa tttacaattt ctttgataaa ttaaaaagtt
      361 attcaaaggg ctacgcgaca cttgattata gtcttagcgg ctataaagct agtaacttag
      421 cgaaaatgga tattttactt aatggtcaac cagttgacgc tttaagtcta attgtgtatc
      481 gcccgactgc ttataaccgc ggtgttgcca tgtgtcgagc cttaaaaaac atcattccac
      541 ggcaacaatt tgaagtaccg attcaagcag ctatcaataa taagattatt gcaagaaccg
      601 atattaaagc agtgcgtaaa gatgtcttag cgaagtgtta tggtggtgat attagtcgga
      661 aacgcaagct tttagaaaag caaaaagaag ggaaaaaacg gatgaaactc gtcggttcag
      721 tggagattcc acaagaagcc ttcatgtcag ttttaagtat tgatgatgat aattagtaaa
      781 atattagtag ttttatagtt ttaggggtca aaacaaacat caattatggt acactttaat
      841 agaggaagaa atgtatgagc actaaccaag gagaacgctt tagtaatgaa cgctatctaa
      901 cgatgaatca agttagtagg gcacttggca caacactgat tgatgatatt tggaaagaag
      961 tattacaata tcgcaatatg ttcagtgtga ccatcccttt acgcacggtt gaacgtaatc
     1021 gtttacgcgt tgtattaact cccaaaatta atgatcgctt aaatcaactt gaccgcagaa
     1081 tctcgcgtat tcaagcacga attcaagaag tttatgttga cgctaatcat caaaaggcac
     1141 ttaaaacctt attaactcgt gatgttttaa aaacaatcgc cgctagttat gaaattaatt
     1201 ttcatgatgg gattgttaat caactcatta atc
//
LOCUS       DAOSEX010000287         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_119488_length_1230_cov_4.265532, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000287 DAOSEX010000000
VERSION     DAOSEX010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119488_length_1230_cov_4.265532"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..>1230)
                     /locus_tag="PK612_04785"
     CDS             complement(139..>1230)
                     /locus_tag="PK612_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95455.1"
                     /translation="VGSYESRVGIWNSVLELSTGIHLWFGRGLHLFNQTLGFYHSGVE
                     SSGGVTNYFSHNGFLEILGQWGLVGLIPYCLGILAIFIIDIYVAVKNYKVGIPALIIF
                     GAFLGYTMVETSTLFDLTIEGVTTTSLVALPALSWLYAYRHPEKNKEIVKSAEEIEYG
                     MPRYDTAKFERQAAKYIALLLGVTTLFAFYSFYINKLEVPIYILFVTLMLVVFITLPR
                     TLANAYQLRHKKRGAIFGLLFSLSVIIGLAALLLYIFTTSPLVVIFAVKILLINLLLS
                     EKFFRTEEDSFKFYLTNVIGRSSILLFAVIISGSLIVFLYRGLTWFILSEIVGLFIVF
                     TIPFIREFDLKKSLANKKMLLLFGRSIGT"
BASE COUNT          434 a          252 c          229 g          315 t
ORIGIN      
        1 atatttggca gcactatctg atccgcagtc atagatgaag ttaaaaccat tattgttaag
       61 atgccccgaa tagaataatc cttgacctac attatgaaag ataaagtgat tcattgattg
      121 tccccaatca aataataatt atgtcccgat actgcgccca aaaagcaaga gcatcttctt
      181 atttgcgagg ctctttttta aatcaaactc gcgaataaaa ggaattgtaa atacgataaa
      241 gagacctact atttccgata agataaacca ggtcagacct cgatataaaa agacaatcaa
      301 agaaccagaa ataataaccg cgaataaaag aattgaagag cggccaataa cattagttaa
      361 gtaaaactta aatgagtcct cctcagttct aaagaacttc tcgcttaata gtagattaat
      421 aagaagtatc tttacagcga agataactac taatggagat gtggtaaaaa tatagagcaa
      481 taaggccgca aggccaataa taacactcaa ggaaaatagt aatccaaaga ttgcccctcg
      541 ctttttgtgc cttaactgat aggcattcgc taatgtccgc ggcaaggtaa taaagacgac
      601 aagcatcaat gtcacaaata ggatatagat tggaacttca agtttattta tataaaaact
      661 atagaatgca aaaagtgtgg taacgcccaa taacaaggcg atatattttg cggcttgtct
      721 ttcgaattta gccgtgtcgt agcgcggcat tccgtattct atttcttccg cgcttttcac
      781 aatctcttta ttcttctctg gatgtcgata agcgtagagc cacgatagcg ctggaagggc
      841 cactaaagag gtcgttgtca cgccttcaat cgtcagatcg aataaggttg atgtctccac
      901 catcgtatag cctaaaaacg ctccaaagat gattaaagcc ggtatcccta ccttgtagtt
      961 cttgacggca acataaatat caataatgaa gatcgctagt attcccaaac aataaggaat
     1021 caagccgaca aggccccact ggcccagaat ctcaagaaag ccattgtggg agaagtagtt
     1081 agtgacgcca ccgctgcttt cgactcccga atgataaaaa ccaagggttt gattaaaaag
     1141 atgaagacca cgaccaaacc aaaggtgtat tccagtagaa agttctaaga cagaattcca
     1201 aatacctact ctagactcgt atgatccaac
//
LOCUS       DAOSEX010000288         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_119908_length_1228_cov_1.916454, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000288 DAOSEX010000000
VERSION     DAOSEX010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119908_length_1228_cov_1.916454"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PK612_04790"
     CDS             <1..708
                     /locus_tag="PK612_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HOH95456.1"
                     /translation="VRYISSGAFADCRALTSLNVVSENNHYDSRENCNGIIETATNTL
                     MVAVKDTVIVESIEALGEEVFSGVDIQSITIPASVTKIGNWAFSDCEKLETVVIEGEL
                     TMINAGTFSNCTSLTSVNIPETVTEIRGSAFYNCKVLPELKLPANLEKIGSWCFSRCT
                     ALPQIIIPRSVTNIDRYAFSSCANLTIYAEATALPDGWHADWNTDDRPVYWYSETANY
                     DGSHWRYVDGVPTVWIE"
     gene            846..>1228
                     /gene="argS"
                     /locus_tag="PK612_04795"
     CDS             846..>1228
                     /gene="argS"
                     /locus_tag="PK612_04795"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOH95457.1"
                     /translation="MIDIRLQELIKNATVKLGLDIDQDTIVIETSKDSAHGDYATNIA
                     MQLARPLRKNPREVATLLINAMDQTPFEKIEIAGPGFINFFLKPETLSKYIATILKEG
                     SNFGNGSKKDTKVNIEFVSANPTGKL"
BASE COUNT          396 a          189 c          261 g          382 t
ORIGIN      
        1 gtacgctata tttctagcgg tgcttttgct gattgtaggg cactaacatc attaaatgtt
       61 gtaagtgaaa ataatcatta cgactcacgc gaaaattgta acggtattat cgaaacggcc
      121 acaaatacat taatggtcgc cgttaaagat acggtgattg tcgaaagtat tgaagcttta
      181 ggagaggaag tgttttcagg cgttgatatt caaagtatta cgattcccgc gagtgtaact
      241 aaaatcggta attgggcatt tagtgactgt gaaaagttag aaacagtcgt aattgaaggt
      301 gaacttacga tgattaatgc tggcacattt tcaaattgta cttccttaac aagtgttaac
      361 attccggaaa cagtgactga aattcgtggt agtgcctttt ataattgtaa agttttaccc
      421 gaacttaaat tacccgctaa tttggagaag attggtagtt ggtgttttag tcgctgtacc
      481 gctttaccgc aaattatcat tcctagaagc gtgacaaata ttgatcgtta tgcctttagc
      541 agttgtgcga atctaacaat ttacgctgaa gcaacagcat taccagatgg gtggcacgcc
      601 gattggaata ctgatgaccg ccccgtttat tggtatagtg aaaccgctaa ttacgacggt
      661 agtcactggc gctatgttga cggggtgcca acggtgtgga tcgaataatc gctttaaata
      721 ataatttata aaagaaaggt gattgttgtt tgttttatag caataatcac cttttttgct
      781 tgtgaaaagt ggatttactt tataatatac tttaaataaa gtatacttag tcgttgaggt
      841 aagaaatgat tgatatccga ttacaagaat taattaaaaa tgcaactgtg aaactgggac
      901 tagatattga ccaagacaca attgtgattg aaacgagtaa agatagtgct cacggggatt
      961 atgctacaaa tattgcgatg caactagcgc gtccgttacg caagaatccg cgtgaagtag
     1021 cgactttact cattaatgcg atggatcaaa cgccatttga aaaaattgaa attgctggtc
     1081 ctggttttat taatttcttt ttaaaaccag aaacattatc aaaatatatt gcgactattt
     1141 taaaagaagg cagtaatttt ggtaatggta gtaaaaaaga tacgaaagtt aacattgaat
     1201 ttgttagcgc taatccgact ggtaaact
//
LOCUS       DAOSEX010000289         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_120298_length_1225_cov_2.959829, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000289 DAOSEX010000000
VERSION     DAOSEX010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120298_length_1225_cov_2.959829"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PK612_04800"
     CDS             complement(<1..804)
                     /locus_tag="PK612_04800"
                     /inference="COORDINATES: protein motif:HMM:NF017046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOH95458.1"
                     /translation="MNKYSNRRQKLYKELANNSALILFSGCEIKKSADGFYDFDVNKN
                     FFYLTGIKQEGSILVVTKIKNIVKETILTSPYDAHKEIWTGRRLKPENVREITGINEY
                     KTTDEFNALLQTLKGNVKTVYLDLEPGIFTHTGLLVSNFREEIINTVNAKVENVYPLI
                     VRLRMVKDEEEIALIRESIALTKKALDFAISKVKPGAYEYEVAAAFEYKIKAAHGVLG
                     FSTIAATGDNAVILHYPDLNTKLEAGELLLLDLGGHNNMYTADISRTVPV"
     gene            complement(797..>1225)
                     /locus_tag="PK612_04805"
     CDS             complement(797..>1225)
                     /locus_tag="PK612_04805"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOH95459.1"
                     /translation="TTVNWGELDFLLIDFPPGTSDVALSAFQTIAVDGIVLVTTPQQM
                     VQQIVKKTYKMGEEMKVKLLGVIENMAYVKLPNSDEKYYLYGQNKLEDLQNTFPINIL
                     GSLPFLPQNTKLVDEGKIEVTDTTEFSAIVEKLIKELQNE"
BASE COUNT          360 a          232 c          186 g          447 t
ORIGIN      
        1 aacgggtaca gtccgcgata tatcggcggt gtacatatta ttatgaccac ctaaatcaag
       61 gagtaataat tcccctgctt caagtttagt gtttaagtca gggtaatgaa gaattaccgc
      121 gttatcacct gtagcggcga tagtactaaa gcccaaaaca ccatgcgccg ctttaatttt
      181 atattcaaaa gcagcggcaa cttcatattc ataagcgcca ggctttactt tacttatggc
      241 aaaatcaagt gcctttttag ttaaagcaat actttcacgg ataagtgcaa tttcctcttc
      301 atcttttacc atgcgcaaac gaacgattaa cgggtagaca ttttcaactt ttgcattaac
      361 ggtattaatg atttcttctc tgaaattgct tactaataaa ccagtatgcg tgaaaatgcc
      421 aggttctaaa tcaagataaa ctgtttttac attacctttt aatgtttgta gcaaagcatt
      481 aaattcatct gttgttttat attcgttaat cccagttatt tctcttacat tttctggttt
      541 taaccggcga cctgtccata tttctttatg ggcatcatat ggactcgtta aaattgtttc
      601 tttcacgatg tttttaattt tcgttacgac taaaattgaa ccttcttgtt taataccagt
      661 taagtaaaag aagttcttat ttacatcgaa atcataaaac ccatcggcgc ttttcttaat
      721 ctcacaacca ctaaaaagaa tgagcgcact attatttgca agttctttat ataatttttg
      781 gcgtctatta ctatatttat tcattttgca actcctttat taatttttcg acaattgcac
      841 tgaattcagt cgtatctgtt acctctattt taccttcatc aacaagtttt gtattttgtg
      901 gtaagaaagg aagtgatcct aaaatattaa tcgggaatgt gttttgtaag tcttctaatt
      961 tattttgacc atataaataa tacttttcat cactatttgg taatttaaca tatgccatat
     1021 tttcgataac accgagaagt ttgactttca tttcttcccc catcttatat gttttcttca
     1081 caatttgttg gaccatttgt tgtggtgttg tcacaagcac aatcccgtca actgcaatag
     1141 tttgaaaagc actaagtgcg acgtcacttg ttcctggtgg gaaatcaatg agtaaaaagt
     1201 ctaattcacc ccaattaaca gttgt
//
LOCUS       DAOSEX010000290         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_122413_length_1211_cov_2.373702, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000290 DAOSEX010000000
VERSION     DAOSEX010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122413_length_1211_cov_2.373702"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1211
                     /locus_tag="PK612_04810"
     CDS             <1..>1211
                     /locus_tag="PK612_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOH95460.1"
                     /translation="IRVYDSKDARQPRGKQTWKATPFECFYHGTSGIYGDVWLEEVDA
                     VYIKCFILTPIAKERKLKVKALFSEESIGAKFTINATFAGKNVAKHEYLITNSIQEFE
                     LLIPGELKLWSTETPHLYDLELCIGSADKVLSYFGFNDVGIRGKYITLNEKETYLKFV
                     LDQGYFWGGDLTATYEDFMIDINYLKAAGFNGVRKHEKVESKLFFYLADRYGLLNWVE
                     YPSPHLFDNNKADVLKEQWTNIIKEHMSHPSVMAYVCYNESWGVHQILTDTDQQKFTV
                     ELYHLTKKIDPHRLAISNDGWEHTESDLLTVHNYQETYEELVKTYENMESDLKTKGNT
                     TANETRKAYAEGYKYQGEPILITEFAGIAIDHNTSDGWGYGKAAIGTEGFLAKLEGQL
                     KAIKKFDFFVG"
BASE COUNT          426 a          182 c          246 g          357 t
ORIGIN      
        1 catacgtgta tatgatagca aagacgccag acaaccacgc ggtaaacaaa cgtggaaagc
       61 aaccccgttt gaatgttttt atcatggcac aagcggaata tatggtgatg tttggcttga
      121 agaagttgac gctgtttaca ttaagtgctt tattcttaca ccaattgcga aagaaagaaa
      181 attaaaggtt aaagcgttat ttagtgaaga aagcataggc gctaaattca caattaacgc
      241 aacttttgct gggaaaaacg ttgcaaaaca tgaatatttg ataacaaaca gtattcaaga
      301 gtttgaattg cttattcctg gtgaattaaa gttgtggagc actgaaaccc cacatcttta
      361 tgaccttgag ttatgcatcg gttcagccga taaagtttta tcttattttg gatttaatga
      421 tgtagggatt agaggcaaat acattacact aaatgagaaa gaaacatatt taaaatttgt
      481 gttagatcaa ggttacttct ggggtggtga tttaaccgct acttatgaag acttcatgat
      541 tgatattaat tatttaaaag ctgctgggtt taatggtgtg cggaaacacg agaaagtaga
      601 aagtaaatta ttcttttatc ttgctgatcg ctatggctta ttaaattggg ttgagtatcc
      661 ttcaccacat ctttttgaca ataataaagc agacgtttta aaagaacagt ggactaatat
      721 tattaaagaa catatgtcac atccttcagt gatggcttat gtttgttaca acgaatcatg
      781 gggcgtgcac caaatcttaa ccgataccga tcaacaaaaa tttacagttg agttatatca
      841 cttaacaaag aaaattgatc cgcaccgttt agctatttca aatgatggtt gggaacacac
      901 agaaagcgat ttactaacgg tccataatta tcaagaaaca tatgaagagc ttgtaaaaac
      961 atatgaaaat atggaaagcg atctaaaaac taaaggtaat acgacggcaa acgaaacaag
     1021 aaaggcttac gctgaaggat ataaatatca gggcgaacca attcttatta ctgagtttgc
     1081 aggtattgcg attgaccata atacgagtga tggttgggga tatggtaaag ccgctattgg
     1141 aacagaaggt tttcttgcaa aattagaagg tcaactaaaa gcaattaaaa agttcgactt
     1201 ctttgttggt t
//
LOCUS       DAOSEX010000291         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_123625_length_1203_cov_2.416376, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000291 DAOSEX010000000
VERSION     DAOSEX010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123625_length_1203_cov_2.416376"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PK612_04815"
     CDS             complement(<1..373)
                     /locus_tag="PK612_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOH95461.1"
                     /translation="MSDKLILGSHVGMSAPDYYIGAINEALSYGANTFMIYTGAPQNT
                     RRLATEQMHALEGIKYLKEKGMDIKHIVAHAPYIINLGNSVKPETYELAVSFLQDELQ
                     RVADFGITMVVLHPGSHVGAGV"
     gene            complement(370..>1203)
                     /locus_tag="PK612_04820"
     CDS             complement(370..>1203)
                     /locus_tag="PK612_04820"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH95462.1"
                     /translation="PTVQLFSATIPQKLANLVTLKTGVKTVIDIDRDDKTSDNVTHHL
                     IDVHHMNRFEVVESFIKHFNPYLLLVFCSTNKEVLELYEFLSAKGHKIGLLTGELESR
                     KRKAMFRRVRNNEFQVVVASDIAARGIDIEHVSDVLSVSFPFDLEFYFHRAGRTGRLD
                     NKGNAYTLYDHDDLETVEKVEKLGVKFTRLVYKGGAFSAVTKRISRKKALTPEEKELN
                     VKIKTAVAETKTKKVRPGYKKKVRTAVENVKRKHRRKMIKKSIREQRVERYKAEKRRD
                     S"
BASE COUNT          349 a          268 c          192 g          394 t
ORIGIN      
        1 cgacacctgc acccacgtgt gaaccaggat gaagcacaac cattgtaatc ccaaaatcag
       61 caacgcgttg taattcatct tgtaaaaaag ataccgctag ttcataagtt tcaggtttta
      121 cactgttgcc taaattaata atataagggg catgagccac gatatgctta atatccatcc
      181 ctttttcttt taaatattta ataccttcaa gtgcatgcat ttgctcagtc gctaagcgtc
      241 ttgtattttg cggtgcacca gtataaatca taaatgtatt tgcgccatag cttaaagctt
      301 cattaattgc gccaatataa taatcgggag cactcatccc aacatgacta cctaaaatta
      361 atttatcact catgaatccc tccttttttc cgctttataa cgttcgacac gttgttcacg
      421 gattgacttt ttaatcatct tacggcgatg ttttcttttt acattttcca ccgctgttct
      481 aactttcttt ttatagccgg gtcgcacttt ctttgttttt gtctcggcga ccgctgtttt
      541 aattttaaca ttaagttctt tttcttccgg agttagtgct ttcttgcgac taatgcgctt
      601 ggttacagca ctaaaagcgc cacctttata cacaagacgc gtaaatttaa cacctaactt
      661 ctctactttt tcgactgttt ctaagtcatc atgatcgtaa agcgtataag cattaccttt
      721 attatctaaa cgccctgttc tacccgcacg gtggaagtaa aattctaaat caaaaggaaa
      781 actaacgcta agaacatcac taacatgttc aatatcaatc ccgcgcgctg caatatcact
      841 cgcaacaaca acttggaatt cattattacg cacccgtcta aacattgctt tacgtttacg
      901 gctttcaagt tcaccagtta aaagtccgat tttatgacct tttgcactta aaaactcata
      961 aagttcaagc acttctttat tcgtggagca aaacactagt aataaataag gattaaagtg
     1021 tttaataaaa ctttcgacaa cttcaaagcg attcatatgg tgtacatcga ttaagtgatg
     1081 ggtgacgtta tcacttgtct tatcatcgcg gtcaatatca atcacggtct ttacacccgt
     1141 ctttaaagta acaaggttcg ctagcttttg cggaatagtc gccgagaaaa gctggacagt
     1201 agg
//
LOCUS       DAOSEX010000292         1200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_124109_length_1200_cov_2.323144, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000292 DAOSEX010000000
VERSION     DAOSEX010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124109_length_1200_cov_2.323144"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PK612_04825"
     CDS             complement(<1..705)
                     /locus_tag="PK612_04825"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HOH95463.1"
                     /translation="MIAKHLDLSRITSLAPSTALVVGTFDGVHIGHKYLVEEGFKAAD
                     NVAVLLVYTNKKLTKLHQKSGVLTSLEDRVEIFEKFGVKTVYLLNLGDELLKLEPCEF
                     INKVLVPLAPNSVIVGTDFRFGRGAKGTGEDLREFGKNVFTTRVLAPLYYENEKVSTS
                     LIKELLSKGKPKIAKSLLGRYYALTGLVTKGYGLGNKLGFPTANIALDPSYFVPAHGV
                     YFVRVKFRANYYFGMAS"
     gene            complement(702..>1200)
                     /locus_tag="PK612_04830"
     CDS             complement(702..>1200)
                     /locus_tag="PK612_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95464.1"
                     /translation="TSAIKIDGKRAYEYARKNIEVDMPSRVVEVYTLEVLEYNHPLLV
                     IEATVSKGTYIRTLAQVIALALGTNATTIKLVRTRHGDFSLEKAKLFEEVSEEDIIDP
                     SLALKKFPIYKLSDEEVKRVRNGMTIKLNASEEIVQIHNNSGVLQALYKKKGDNYIAL
                     RGFNL"
BASE COUNT          365 a          265 c          177 g          393 t
ORIGIN      
        1 acttgccatc ccaaagtaat aattagctct aaacttaacg cgaacaaaat atacaccgtg
       61 cgctggtaca aagtaacttg ggtcaagggc aatattcgca gtcggaaatc ctaatttatt
      121 tcctaaaccg tagcctttag tcactaaccc agtgagcgca taatagcggc caagtaatga
      181 ctttgcgatc ttaggtttac ctttacttaa aagctcttta attagtgatg tgctcacttt
      241 ttcattttca tagtaaagtg gcgctaaaac tctagtcgta aatacatttt taccaaactc
      301 tcgcaaatct tcgccagtgc ctttagcgcc ccgaccaaat ctaaaatcag taccaacaat
      361 aacagaattt ggagctaaag gtactaaaac tttattaata aattcacatg gttcaagctt
      421 gagtaattca tcacctaaat ttaataaata aacagtcttc actccaaact tctcaaagat
      481 ttcaacacgg tcttctaaac tcgttagcac cccacttttt tgatgaagtt ttgtaagttt
      541 tttattagtg tatacaagta aaaccgcaac attatctgcc gctttaaaac cttcttccac
      601 taaatactta tgaccgatat ggacgccgtc aaacgtccca acaactagtg cagtgcttgg
      661 tgctaaagat gtaatacgac ttaaatcaag atgtttagca atcataaatt aaatcctcgt
      721 agcgcgatat aattatcgcc ttttttctta tataatgctt gcaaaacgcc actattatta
      781 tgaatttgta caatttcttc actagcgtta agtttaatcg tcatcccgtt tcgcaccctt
      841 ttaacttcct catcacttaa cttgtaaatc ggaaactttt ttaacgctag acttggatca
      901 atgatatctt cttcacttac ttcttcaaaa agttttgcct tttcaagact aaagtcacca
      961 tgccttgtgc gcactagctt aatcgttgta gcgtttgttc ctaaggcaag cgctataact
     1021 tgagcaagcg ttcgaatata agtgccttta ctaacagttg cctctatgac tagtaaagga
     1081 tgattatatt caagtacttc tagagtgtaa acttctacta cacggcttgg catgtcgact
     1141 tcaatatttt tacgcgcata ttcataggcc cttttcccat caattttaat cgcacttgtc
//
LOCUS       DAOSEX010000293         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_124549_length_1197_cov_3.050788, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000293 DAOSEX010000000
VERSION     DAOSEX010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124549_length_1197_cov_3.050788"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PK612_04835"
     CDS             complement(<1..700)
                     /locus_tag="PK612_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOH95465.1"
                     /translation="MAKELIAMILAGGKGTRLYELTRKDAKPAVHFGGKYRIIDYALS
                     NCAHSGIDTVGVLAQYESISLSRYIGNGEKWGLNGIRSSTVTIAPRQTDSGSNWFIGT
                     ADAIYQNLEFIDSYNPENVLILSGDHIYRMDYSKMLETHLENNADLTISVIEVTLEEA
                     KRFGILSADEDGRITEFVEKPPKPKSTLASMGIYIFNYKVLREALVKDAADENSSHDF
                     GKNIIPTLLANNNRL"
     gene            complement(705..>1197)
                     /locus_tag="PK612_04840"
     CDS             complement(705..>1197)
                     /locus_tag="PK612_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95466.1"
                     /translation="VKSGAIKGVAPYEFNKRLFHVVYVYDDEKYSAIIHLPSIDSPYY
                     IQIHNGNFAPPEAYADNLIVKIAYVSPYKLQYYASSSATNAKIYLRKENETRFVKFIK
                     IYQATYQYVTTFTDEYLKVEGLTSPGRLRLGEKYDNKHYHELLTRLRLLQALMSAMLE
                     RGK"
BASE COUNT          354 a          244 c          203 g          396 t
ORIGIN      
        1 ataagcgatt attattcgct aataaagttg gaatgatatt tttaccgaag tcatgggaac
       61 tattttcatc agcggcatct ttaacgagtg cttcgcgtaa tactttatag ttaaagatat
      121 aaatacccat actcgctagt gttgattttg gttttggtgg tttttccaca aattcagtaa
      181 tccgtccgtc ttcatcagcg ctaagaatcc caaagcgctt agcttcttca agtgtgactt
      241 caatgacaga aattgttaaa tcagcgttat tttctaaatg tgtttcaagc attttcgaat
      301 aatccatccg ataaatatgg tcaccactta aaattaaaac attttcggga ttatacgaat
      361 caataaattc caagttttga taaatcgcat cagcagtacc aataaaccaa ttactgccgc
      421 tatcagtttg ccggggcgca attgttactg tactacttct aatcccgttt aatccccatt
      481 tttcgccgtt acctatataa cgactaagac taatcgattc atattgggct aagacaccaa
      541 ctgtatcgat gccactatgc gcacagttac ttaaggcata gtcaatgatg cggtattttc
      601 cgccaaaatg caccgctggt ttagcgtctt tgcgggttag ctcgtagaga cgtgtccctt
      661 tacctcccgc taaaatcatt gcgataagct cttttgccat ccttttactt ccccctttca
      721 agcatcgctg acattaaggc ttgtaacaag cgtaatctcg ttaaaagctc atggtaatgt
      781 ttattatcat atttttcacc tagccgtaat cgacccggtg aagttagccc ttcaactttt
      841 aaatattcat cagtgaaggt tgtcacatat tgataagtcg cctgataaat tttgataaat
      901 ttaacaaagc gcgtttcatt ttcttttcgt aaatatattt tagcatttgt ggcactactt
      961 gaggcatagt attgtagttt gtagggagaa acatacgcaa tctttacaat taaattatca
     1021 gcgtaagctt caggtggagc aaaattaccg ttatggattt ggatgtaata aggtgaatca
     1081 atacttggca agtgaataat cgcactatat ttttcatcgt catacacata tacaacatga
     1141 aaaagccgct tattaaattc gtaaggcgca acacctttaa tcgccccact tttaaca
//
LOCUS       DAOSEX010000294         1191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_125482_length_1191_cov_2.972711, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000294 DAOSEX010000000
VERSION     DAOSEX010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125482_length_1191_cov_2.972711"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1191
                     /locus_tag="PK612_04845"
     CDS             68..>1191
                     /locus_tag="PK612_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95467.1"
                     /translation="MDVLKEDGLAASTMVNYPTQMQILAKPPTDNARTVQWPYYVSVT
                     PLPFGVFTQLPGDEAYITEFYDVIGKDSKFATERNEIMFVLDKYNRISVQALERMGIV
                     DNSANVQKLNFDDIIGREFKMVHNDDIYKKALGNIDDYVVRDFMGQSRTIEKYRFQNP
                     QYLYDDPTVGEMLKITGILRPKPGIQFEMLAPGMVYTQALNKYMHETNKHNKVAKAQV
                     NNVVIKEANITAENINTAIEFYSATTDWSGVKPVNPTTIRIDKFLSNLKIYGVDLADI
                     SKDNAIYEKLLKELLNTAYSPVESVAIFAKDGDSKKEIKARINEYNNTREDEKDKIVI
                     FDAVEFVTDSIATMIDVVSIVLIVFTSISLVVSGFLIGIIT"
BASE COUNT          429 a          181 c          229 g          352 t
ORIGIN      
        1 attttcatta taaacaacac ggattaccat tacaaataat aggataacat ctgaattctt
       61 agatttagtg gacgtattaa aggaagacgg gcttgccgct agtacaatgg ttaattatcc
      121 aacacaaatg caaattttag caaagccgcc cactgataac gcgcgtactg tacagtggcc
      181 ttattatgtc agtgtgacgc cactaccctt tggggtattt acccaattac caggggacga
      241 agcctatatt actgaatttt atgatgtaat tggtaaagac tcaaagtttg cgacagagcg
      301 taatgaaatt atgtttgtac ttgataaata taatcgtatc tcagtccaag ctttagaacg
      361 gatgggcatt gttgataatt ccgcaaatgt acaaaagctt aattttgatg acattattgg
      421 gcgagaattt aaaatggtgc acaacgatga tatttataaa aaggcattgg gtaatattga
      481 tgactatgta gtgcgtgact ttatggggca atcacgcacg attgaaaagt atcgttttca
      541 aaacccacaa tatttatatg atgacccaac ggttggtgaa atgctgaaaa ttactggcat
      601 tttgcggcca aaaccgggaa ttcagtttga aatgctcgcg ccaggaatgg tgtatacaca
      661 agcattaaat aaatatatgc atgaaacaaa taaacataac aaagttgcaa aggcacaagt
      721 taataatgta gtaatcaaag aagcaaatat cactgcagaa aatattaata ctgcaattga
      781 attttatagc gctactaccg attggtcggg tgtaaaacca gttaatccaa caacaataag
      841 gattgataaa tttttaagca acctcaaaat ttatggagtg gacttagcgg atataagtaa
      901 ggacaacgcg atttacgaaa aactattaaa agaattatta aatacagctt attcaccagt
      961 agaatcagta gcaatttttg ctaaagacgg tgattcaaaa aaagaaatta aagcacggat
     1021 taatgaatat aataatacgc gcgaagacga gaaagataaa attgtaatct ttgatgcagt
     1081 agagtttgtt actgattcaa ttgcgacaat gattgatgtt gtttcaattg tattaattgt
     1141 atttacaagt atctcacttg ttgtgtcagg attcttaatt ggcattatta c
//
LOCUS       DAOSEX010000295         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_126274_length_1186_cov_3.375774, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000295 DAOSEX010000000
VERSION     DAOSEX010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126274_length_1186_cov_3.375774"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PK612_04850"
     CDS             complement(<1..789)
                     /locus_tag="PK612_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOH95468.1"
                     /translation="MINRKLLKLIDNHKHIIFTVILMVCGLITNIVITGSLVYLIKEV
                     ANHSDDSSVWTYLWPALIAIISIIIRYLLSISVGYLTDIIGRQAKKDLRQKVYNKIVR
                     LGVRTTDGMKVAGLTQVSLEGVEQLDLYYSTYIPQFFYALLAPIILFFVTVWFDWRFS
                     LVLLAAVPLIPLSIIAISKYAKRIFAKYWGKYTAMGDKFLDSIQGLRELKIFKADAYQ
                     QTKMNEASEEFRVITMKVLVMQLASTTIMDIVAYTGAGIGVLIAI"
BASE COUNT          401 a          207 c          178 g          400 t
ORIGIN      
        1 tattgctatt aatacaccaa ttccagcgcc agtataagcg acaatatcca taatcgttgt
       61 actcgctaat tgcatgacta acactttcat agtaatcaca cgaaattctt cacttgcttc
      121 attcatcttt gtttgctgat aagcatcggc cttaaagatt ttaagttcgc gtaatccttg
      181 gatactatcg agaaatttat cacccattgc tgtgtatttt ccccagtatt tagcaaagat
      241 ccgttttgcg tatttagata tagcgataat ggaaagtgga attaggggta ccgctgctag
      301 tagcacgagt gaaaaccgcc aatcaaacca taccgttaca aaaaacaaaa ttattggcgc
      361 taatagtgcg tagaaaaatt gtggaatata agttgagtaa tataaatcta attgctccac
      421 gccttcaagt gaaacttgcg ttaaaccagc caccttcatc ccgtcagtag ttctcacgcc
      481 aagtcttaca atcttgttat acactttttg acgcaggtct ttttttgctt gacgaccaat
      541 aatatctgtc aagtaaccaa cactaattga aagtaaataa cgaataataa ttgaaataat
      601 agcaattaat gcaggccata aataagtcca tacactacta tcatcactat gattagcgac
      661 ttcttttatt aaataaacaa gcgatccagt tattacaata tttgtaatta atccgcatac
      721 catgagtatc acagtgaaaa taatatgttt gtgattatca attaatttca gtaactttcg
      781 attaatcatt tatgctttat aactcctaat tacttaagct tattagttag cgaacgctaa
      841 cctttatggt aaaaaagaag gcggcgatat tcttgttagt cgccgctaac ttcgtactaa
      901 ctatacattc catcttgtca attgtcaatg ataactataa tattcatcct tgagttttgt
      961 ctgtaaatta caataaaagt aatgatacga gcattataaa aagatactat tattaagtca
     1021 ctaatgtaga atacactact ttattagaat tattgaagtt cacatcattg atttttcagt
     1081 agtgttatgt tattttgaat gtagagaatt tatatttaag gagtcggtta aatgggtcta
     1141 aaaccatctg gtgagatgta tttaaaaaca atttatattt taacta
//
LOCUS       DAOSEX010000296         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_126804_length_1183_cov_2.070922, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000296 DAOSEX010000000
VERSION     DAOSEX010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126804_length_1183_cov_2.070922"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..>1183
                     /locus_tag="PK612_04855"
     CDS             103..>1183
                     /locus_tag="PK612_04855"
                     /inference="COORDINATES: protein motif:HMM:NF017926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005940 - septin ring [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HOH95469.1"
                     /translation="MKVVTLLNTLDIVFIVASIILVLAVFGFILSKFAFRGTKNKKAL
                     KSLSIKYSYVRSLLLGENIQYIKRIEAISDINLLMRPAYEEFFTRFEQLRNVDDRKAS
                     RSIQILNEALAAGNKEFKKAFNEHRPIILDYERKVTALSKDLTNMIKPESEIREESVY
                     DKEKLRIIKNIYRDHKDELEICVPSFEKIFTQIELLFKEYDNALNGAYYDEARSYLEK
                     IRTFNDQLERILQILPLLVVKATVLIPEKIATLKKSYEEATAYNVPLHHLRVSAEIES
                     FNEQLEVIKKQLIRFDTRNVEQTLNQIADTIDELINSFNVEIEKQREFTAEYDKTYLH
                     VNELEQRYIRLVNRMPQVKEYYLINP"
BASE COUNT          443 a          177 c          194 g          369 t
ORIGIN      
        1 ctaatagccg cttattatca agtatttaac ataataaata tatgttaacg tatgaaaaca
       61 ttttcaaaca ttgtataata tacaataagt gaggtgcgac ttatgaaagt agtaacatta
      121 ttaaacacac tcgatattgt cttcattgta gcaagtatca ttcttgtcct agcggttttc
      181 ggatttattt taagcaaatt tgccttccgt ggcacaaaaa ataaaaaggc attaaaatct
      241 ttatcaatta aatattcata tgtgcgcagc ctactccttg gtgaaaatat tcaatatatt
      301 aaacgtatcg aagcaataag tgatattaat ttactaatgc gtccagctta tgaagaattc
      361 tttacgcgct ttgaacaatt acgcaatgtc gatgaccgta aagcaagtcg tagtattcaa
      421 atattaaatg aagcactcgc ggcaggtaat aaagaattta aaaaagcttt taatgaacat
      481 cgccccatca ttcttgatta tgaacgtaaa gtcacagcgt taagtaaaga tttaacgaat
      541 atgattaaac ccgaatcaga aattagggaa gagagtgttt acgataaaga gaaattacgg
      601 attattaaaa atatttatcg tgatcataaa gatgaactgg aaatttgtgt accaagcttt
      661 gaaaaaatat ttacacagat tgaattactc tttaaagaat atgataatgc tcttaatggt
      721 gcttattatg acgaagctcg tagttattta gaaaagattc ggacatttaa tgatcaactt
      781 gaacgcatct tacaaatttt accgctttta gttgtgaagg ccacagtgtt aatccctgaa
      841 aaaattgcga cattaaagaa gagttatgaa gaagcaacgg cttataatgt tccacttcac
      901 catttacgtg tcagtgctga aattgaaagc tttaatgaac agctcgaagt gattaaaaaa
      961 caacttattc gttttgatac acgcaatgtc gaacaaacac ttaatcaaat tgccgataca
     1021 attgatgaat taataaatag tttcaatgtt gaaattgaaa aacaacgcga atttacggca
     1081 gaatacgata aaacatattt acatgttaat gaattagaac aacgctatat tcggcttgtt
     1141 aatcgaatgc cgcaagtgaa agagtattat ttaattaatc ctg
//
LOCUS       DAOSEX010000297         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_127178_length_1181_cov_1.611901, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000297 DAOSEX010000000
VERSION     DAOSEX010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127178_length_1181_cov_1.611901"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PK612_04860"
     CDS             <1..578
                     /locus_tag="PK612_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95470.1"
                     /translation="KNIYRSSKHYYDESATLVNVDTSGNHFPIYITDESFTDNHPDNN
                     EPYYFIVLTSKNGLGTIISYPLISQEHLIGSTSARLEVKAGVPFLIVKLNGVAPENFD
                     KFRLVVKDNVVDIHFAEYDANLGVGEYHFDLTQIRWKGIWYDILIEYKSNSNRYDLLA
                     ESADLTQAISVGGDVYKFEEWEGLLKVHKVK"
     gene            580..>1181
                     /locus_tag="PK612_04865"
     CDS             580..>1181
                     /locus_tag="PK612_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylceramidase"
                     /protein_id="HOH95471.1"
                     /translation="MLKLIRSTKCGERLKEIGKLNATKEVKSNRFLIVDEKITYQTHA
                     GFGGALTEATTSVLNKMSQAQKESVLAAYFGKNGLNYNLARITINSSDFGYGNYDYLD
                     GADASLESFTLKNEETRAIPAIKEVNKIHGEKIKLFASPWSPPAFMKTTKTMNEGGKL
                     LPEHYSTWAKYFALYLKGMESYDLKVDYISIQNEPEAKQR"
BASE COUNT          403 a          179 c          255 g          344 t
ORIGIN      
        1 gtaaaaatat ttatcggagt agtaaacatt attatgatga aagtgctacg cttgttaatg
       61 ttgatacaag tggtaatcat tttccgattt atatcactga cgaatcattt acggataatc
      121 atcctgataa taatgagccg tattacttta ttgtgttaac gagtaaaaac gggcttggga
      181 caattattag ttatccgctg attagtcagg aacatttaat tggttctact tcagcaaggc
      241 ttgaagtaaa agctggagtg ccgttcttaa ttgtgaaatt aaacggtgta gcgcctgaaa
      301 actttgataa attccgtctt gttgttaaag ataatgttgt cgacattcat tttgcggaat
      361 atgacgcgaa tctaggggtc ggcgaatatc acttcgactt aacacaaatt agatggaagg
      421 ggatatggta cgacatcctt attgaatata aaagcaatag taatcgctat gacttacttg
      481 cggaaagcgc tgatttaacg caagcaattt ctgtcggtgg tgatgtatat aaatttgaag
      541 aatgggaagg gctcttaaaa gtgcataagg taaagtaata tgttgaaatt aattcgtagt
      601 acaaagtgtg gtgaaagatt aaaagaaatt ggtaagctta acgctacaaa agaagtgaag
      661 agtaatcgct ttttaatcgt tgatgaaaaa ataacttatc aaactcatgc ggggtttggc
      721 ggggcattaa ctgaagcaac gacctcagtg ttaaataaaa tgtcacaagc acaaaaagaa
      781 agtgttttag cagcctactt cggcaaaaac ggcctaaatt acaacttagc gcgaattact
      841 attaactcca gtgactttgg atatggtaat tatgattacc ttgacggggc ggatgcttcg
      901 ctagaaagct tcaccttaaa gaatgaagaa acacgggcga ttccagcgat taaggaagta
      961 aataaaattc acggtgaaaa gattaaactt tttgcctcac cgtggtcacc gccagcgttt
     1021 atgaaaacaa caaaaacgat gaatgaaggc ggtaaacttt tacctgagca ttatagtact
     1081 tgggctaaat actttgcgct ttatttaaag ggtatggaaa gttatgacct taaagttgat
     1141 tatattagca ttcaaaatga gccagaagct aaacagcgct g
//
LOCUS       DAOSEX010000298         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_127808_length_1177_cov_2.629234, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000298 DAOSEX010000000
VERSION     DAOSEX010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127808_length_1177_cov_2.629234"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PK612_04870"
     CDS             complement(<1..793)
                     /locus_tag="PK612_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HOH95472.1"
                     /translation="MKTFWFIVGSQSLYGEETLKQVEKNAKKIAKGLKTKYPIVYKET
                     VKTHAEAMRLIKDANYDENCLGIITFCHTFSPSKMWVNALQNLQKPWCHFHTQFYDEI
                     PNDAIDMDYMNLHQSAHGDREHGFIGTRLQKNRKIIVGYWQDPKTSEAISAWQDVVDA
                     VNESKTLSVVRFGDNMREVAVTEGDKVEAQMKFGWQVNTIGVGTLVDTIKAVTNEELT
                     AKLAEYKAKYALNTDNFASIEYQAKAEVAIKKILDANNAKAFTNTF"
     gene            complement(797..>1177)
                     /locus_tag="PK612_04875"
     CDS             complement(797..>1177)
                     /locus_tag="PK612_04875"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOH95473.1"
                     /translation="AVAKADGNVINLKNIEPGKQAYNLAKLTGKEAELVEAIIDLKPG
                     MDILLNKEHVKVDYLLGHGGYFKSKSAGRKVMSETLGIPIRLLETAGEGGPWGMAVLA
                     AYRYYNKEQKIKFDKFIEKVFKGA"
BASE COUNT          317 a          246 c          181 g          433 t
ORIGIN      
        1 cgaatgtatt agtgaatgct ttagcgttat tagcgtcaag aatcttttta atcgcaactt
       61 ctgctttagc ttgatattca attgatgcaa agttatcagt gttcagtgca tactttgctt
      121 tatattccgc tagttttgct gtaagttctt cgttagtaac ggctttgatt gtatcgacta
      181 atgtaccaac accaatcgta tttacttgcc aaccaaattt catttgcgct tcaactttat
      241 caccttctgt aaccgctact tcacgcatat tatcaccaaa gcgaacgaca cttaatgtct
      301 tactttcatt aactgcatca acaacatctt gccaagcaga aattgcttcg cttgttttag
      361 gatcttgcca gtaaccgaca attattttac gattcttttg taaacgagta ccgataaaac
      421 cgtgttctct atctccgtgc gctgattgat gaagattcat gtaatccata tcaatcgcgt
      481 cattaggaat ttcatcataa aactgtgtgt ggaaatgaca ccatggtttt tgtaaatttt
      541 gtaaggcatt cacccacatt tttgatggcg agaatgtgtg acaaaaagta atgataccaa
      601 ggcaattttc atcataattt gcgtctttaa ttaagcgcat tgcttctgca tgtgtcttaa
      661 ctgtctcctt atagacgata ggatatttcg ttttcagtcc ttttgcaatt ttctttgcat
      721 ttttttctac ttgcttgagg gtttcttctc catataaaga ttgtgaccca acgataaacc
      781 aaaatgtttt cataaattac gctcctttaa atactttttc tatgaattta tcaaatttta
      841 ttttttgctc tttattgtaa taacgatatg ctgctaagac agccatcccc cacgggcccc
      901 cttcaccagc ggtctctaat aaacgaattg gaataccaag tgtttcactc atcactttcc
      961 tacccgcgct ctttgattta aagtatccgc catgccctaa taaataatca actttaacat
     1021 gctctttgtt tagtaagata tccatgcccg gttttaagtc aattattgct tcaacaagtt
     1081 ctgcttcttt tccagtaagt ttagcaagat tataagcttg ttttccgggt tcaatatttt
     1141 ttaggttaat aacatttcca tcggctttgg ccacagc
//
LOCUS       DAOSEX010000299         1175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_128142_length_1175_cov_2.798214, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000299 DAOSEX010000000
VERSION     DAOSEX010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128142_length_1175_cov_2.798214"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PK612_04880"
     CDS             <1..477
                     /locus_tag="PK612_04880"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOH95474.1"
                     /translation="TLREMYDFILKTLRNNNYIRYEVSNFALKGYESIHNKLYWENRN
                     YYGIGLGASGYIDGKRYLISGGLTKYLKGESIINYDEITSEEYVEEYLMLNLRLAQGF
                     SLQDFKTRVGFDFLEKYGTKVKSLIERGLLNVTDDKVYATDEGIMLLDQVVLTLIM"
     gene            557..841
                     /locus_tag="PK612_04885"
     CDS             557..841
                     /locus_tag="PK612_04885"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH95475.1"
                     /translation="MDKVVDIERLRDFFKVLGDDTRMRIILLLEESAKNVTEIANALN
                     MTQSAISHQLRTLYDHRVVTYSKAGQERIYEILDPHIYEIIKQARDHIRE"
     gene            846..>1175
                     /locus_tag="PK612_04890"
     CDS             846..>1175
                     /locus_tag="PK612_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOH95476.1"
                     /translation="MIQDNKQSKFQKIFHELGLYAYFIGLLLFLFALLAFIPGVSFLS
                     DKAVMILNLIALILAGYEVISEGLVEMVKESIKSKRFRPNIHILMALGAIGAIIIGDY
                     GDAALLIL"
BASE COUNT          407 a          157 c          230 g          381 t
ORIGIN      
        1 actttacgcg agatgtatga ctttatttta aaaacattaa gaaataataa ttatatacgc
       61 tatgaagtta gtaattttgc gttaaaggga tatgaaagta tccacaataa actatattgg
      121 gaaaaccgta attattacgg gattggcctt ggcgctagtg ggtatattga tggtaaacgc
      181 tacttaataa gtggcggttt aactaagtat ttaaaaggcg aaagtatcat aaattacgac
      241 gaaataacaa gcgaagaata tgttgaagag tacttaatgc ttaacttacg actcgcacaa
      301 ggttttagtt tacaagactt taaaactcgt gtcggctttg attttttaga aaagtatggc
      361 actaaggtaa aaagcctcat agaaagagga cttttaaatg tcactgatga caaagtatat
      421 gcgacagatg aagggattat gctccttgat caagtcgttt taacattaat aatgtaaata
      481 tctttacata tattagatat attttttata atgtatatga acacttgctc aaatattcaa
      541 gtgttaggag aaaattatgg ataaagtcgt ggatattgaa agattacgag acttctttaa
      601 agtcttagga gatgatacgc gtatgcgtat tattttactc ttagaagaaa gcgcaaagaa
      661 tgtcactgaa atcgctaatg cattaaatat gacgcaaagt gcgattagtc atcaattacg
      721 gacactttat gaccatcgcg tagtcacgta ttcaaaagct ggccaagagc ggatttatga
      781 aattcttgac ccgcatattt atgaaattat taaacaagca cgtgaccata ttagggagta
      841 aaaatatgat acaagataat aaacaaagta aatttcaaaa aatatttcac gagcttggtc
      901 tctatgctta ttttatcggg ctattgctct ttttatttgc acttttagca tttatccccg
      961 gtgtgagttt tttaagtgat aaagcggtaa tgatattaaa cttaatagcg cttattcttg
     1021 cgggttacga agttattagt gaaggactag tcgaaatggt taaagagagt attaaaagta
     1081 aaagatttcg tccaaatatt catatcttaa tggcactagg ggcgattggg gcgattatta
     1141 ttggtgatta tggcgacgct gctttactta tatta
//
LOCUS       DAOSEX010000300         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_128470_length_1173_cov_3.291592, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000300 DAOSEX010000000
VERSION     DAOSEX010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128470_length_1173_cov_3.291592"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..>1173)
                     /locus_tag="PK612_04895"
     CDS             complement(28..>1173)
                     /locus_tag="PK612_04895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOH95477.1"
                     /translation="TANDTIILKASILDVISGDRVSIKRVFQSGDITAVSTLPTIELF
                     DGTSVVLANSIYKMTAPEGETPTPDVSFNTGTVSSDGTLTLRLQFKDDDVFDNYRFRL
                     MIHDKSTDIGYVAYNRTLVGSLTAESNETDSFEFSAIDFGVLEFIPKTDKLNIVFGDT
                     ITDTDNSVLEYNAGSQSANRKLSVLESRITALDNPFRDKVIAIWGDSRESNNPTSDPS
                     GVGDQKDTSYPALLAKKLGATVLNFGLSGGAWAENTVQQDAASAIVNRVLTEDTSASA
                     DVIIISSMNDFKLATPLGSPTEATDKTTFYGAMRMTYKRLANKYPSKKIWLVLPQKRF
                     DESTNYGGGDYYAYRKAQIDVAREYGIPTIDLYNNFPNSKTEISGGV"
BASE COUNT          326 a          251 c          218 g          378 t
ORIGIN      
        1 gtcattcagc atattagtat cgtagaatta tacgccacca cttatttcgg ttttgctatt
       61 tgggaagtta ttgtaaaggt caatggttgg aataccatat tcccttgcga catcaatttg
      121 tgccttgcga taagcataat aatcgccacc accataatta gtgctttcat caaagcgttt
      181 ttgtggcaat actaaccata tctttttgct tggatattta tttgccaagc gtttataggt
      241 cattcgcatc gcaccataga aagttgtttt atcggttgct tcggttggac taccaagtgg
      301 tgtcgctaac ttaaagtcat tcatacttga aatgatgata acatcggcac tcgcacttgt
      361 atcttcggtt aagacacgat taacaatagc agaagcagcg tcttgctgaa cggtattttc
      421 cgcccaagca ccaccactca aaccaaagtt tagaacggtt gctccaagtt ttttggcaag
      481 taaggctggg tatgaagtat ctttttggtc gcccacacca cttgggtcgc ttgttgggtt
      541 gttgctttct cgactatcac cccatatcgc tatgacttta tctctaaatg gattgtcaag
      601 ggcggtaatg cgactttcaa gaacacttaa ttttctattt gccgattgac tacccgcatt
      661 atattcgagg acactattat cggtatcagt aatcgtatcg ccaaacacaa tatttaattt
      721 gtcggtcttt ggtataaact ctaatacacc aaaatcaatg gcactaaact cgaaactatc
      781 ggtttcattt gattcggcag ttaaactacc aaccaatgtg cgattatatg caacataacc
      841 aatatcggtg gacttatcgt gtatcatcaa acggaagcgg taattatcga acacatcatc
      901 atctttgaat tgtaatctca atgtgagtgt gccatcactt gataccgtgc cagtattaaa
      961 actaacatct ggtgtcggtg tttcgccttc gggtgctgtc atcttgtaaa tgctattagc
     1021 caacacaaca cttgtgccat caaataattc aatggtcggt aatgtgctga ccgcagtaat
     1081 atcgccactt tggaatacac gcttaattga cactctatcg ccactaatga catctaatat
     1141 acttgctttt aagataatcg tgtcattagc agt
//
LOCUS       DAOSEX010000301         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_129238_length_1169_cov_1.580790, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000301 DAOSEX010000000
VERSION     DAOSEX010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129238_length_1169_cov_1.580790"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..834
                     /gene="upp"
                     /locus_tag="PK612_04900"
     CDS             208..834
                     /gene="upp"
                     /locus_tag="PK612_04900"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOH95478.1"
                     /translation="MVFEIKHPLIDVKLSIMRDEKTKSKEFRESLDEIASLMSYEIFK
                     DIGTHASEDTFMTPTGVELQKIKLDNKIILVPILRAGVGMVDGIRNMIPTARIGHIGI
                     YRDEKTLEPHEYYFKLPKVDNPLVVLVDPMLATGGTAIASIDAIKARGYNNIRMATLV
                     ASPLGIERMSIAHPDVHIYVAAIDEKLNEHGYIIPGLGDAGDRIFGTR"
     gene            840..>1169
                     /locus_tag="PK612_04905"
     CDS             840..>1169
                     /locus_tag="PK612_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011167101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HOH95479.1"
                     /translation="MSFSKDACIFSLTANKELTREVAKILGVNVSEAIVTHFADGETL
                     CEPTSSFRGKNCYVIQSTCKPVNERLMEILIFADALKRASAKSINVVIPYFGYARQDR
                     KNKPRQPI"
BASE COUNT          384 a          185 c          236 g          364 t
ORIGIN      
        1 catgatatta gtgaaaaggt tcatagtcaa gtggaagcaa attttaaagt tttacattgc
       61 catgtccata ttaaccccca ctaaataacg attatttgta aagattattc ggtttttaac
      121 ttattacaaa atttttaata gttaaagacc cttttttaga tttatattaa gtataatata
      181 catgtatgat ttcaatagga gggacttatg gtttttgaaa ttaagcaccc gctgattgat
      241 gtaaagttat caatcatgcg tgatgagaaa acaaaatcta aggaatttcg tgaatcactc
      301 gatgaaattg cttcgctgat gtcttatgaa atatttaaag acattggtac ccacgcttca
      361 gaggacacat ttatgactcc gacaggcgta gagttacaaa aaattaagtt agataataag
      421 attattctag ttccaatctt aagagcgggg gttggaatgg ttgacggcat tagaaatatg
      481 ataccgactg ctagaattgg tcatattggt atataccgtg atgaaaaaac attagaacca
      541 catgaatatt attttaaatt accaaaagtc gataatccgt tggttgtgtt agttgaccca
      601 atgctagcga ctggtgggac ggcaatagcc tcgattgatg cgattaaagc tcgtggttat
      661 aacaatattc ggatggcaac acttgtggcg agcccgcttg ggattgaacg catgagcata
      721 gcgcaccccg atgttcatat ttatgtcgcc gcaattgatg aaaaattaaa tgaacatggt
      781 tacatcatcc ccggacttgg tgatgctggt gaccggattt ttggaacgag gtagagatta
      841 tgagctttag taaggatgct tgtatatttt cattaaccgc taataaagaa ttaacgcgtg
      901 aagtagcaaa gattttagga gtaaatgttt ctgaagctat tgtcacacat tttgcagatg
      961 gggaaacact ttgtgaacct acaagttcat tccgcgggaa aaattgttat gtgattcaat
     1021 ctacttgtaa accagtaaat gaacgattga tggaaattct cattttcgct gatgctttaa
     1081 aaagagcttc agccaaatca ataaatgttg tgattcctta ttttggctac gctagacaag
     1141 accgtaaaaa taaaccacgt caaccaatt
//
LOCUS       DAOSEX010000302         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_129586_length_1167_cov_1.997302, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000302 DAOSEX010000000
VERSION     DAOSEX010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129586_length_1167_cov_1.997302"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1167
                     /locus_tag="PK612_04910"
     CDS             <1..>1167
                     /locus_tag="PK612_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95480.1"
                     /translation="EIYKVVASGENKVLSDAFVIDSLFIKETFSPTNYTPATATAKGH
                     FVGIDNLFDAIITGTQLVGKAKLDEGGHDIRESYGASLSTDNDDVALKIALRNKLNDV
                     LTTLILREATTTKNGLMSSADKVKLTDLPTKGQLDSALAVKVPQTRKILGIDLTNDIL
                     LDEFKTAIGEATAILSGLMSATDKARLDALHALLGVESDADVVVNTINEVLAIFNNYQ
                     EGADLVNALSGKVDKVAGKGLSANDFTDILKAKLDSLLSGSNYYDKTYIDALKDLNGW
                     SSALLTETALTNGGVITKATLSDYDVIKLFVINTATGEIDTDSFDTSIGLVDNYKYVF
                     FDNDGIYLSIANPNCTFTDNITGYQLKIIGQKYEAQDAENVKYDRTVKNLLEAEN"
BASE COUNT          396 a          190 c          237 g          344 t
ORIGIN      
        1 gagatttata aagttgttgc aagcggggaa aataaagttt taagtgatgc attcgtaatt
       61 gatagtttat ttatcaaaga aacatttagc ccgaccaatt acacgccagc aactgcaacc
      121 gcaaaaggac attttgtcgg cattgataat ttgttcgatg ctattattac gggaacgcaa
      181 cttgtcggca aggcgaaatt agatgagggc ggacacgata tacgagaaag ttatggtgct
      241 tcgctttcta ccgataatga cgatgtggca ttaaaaattg cattacgcaa taaattaaat
      301 gatgtcttaa ccacacttat tttaagagag gcaaccacaa ccaaaaatgg tctaatgtca
      361 tccgccgata aagtgaagtt aactgattta cccaccaaag ggcaattaga tagtgcgtta
      421 gcagtcaaag ttccgcaaac tcgcaagata ctcggcattg atttaaccaa tgatatttta
      481 cttgacgagt tcaaaaccgc aatcggcgaa gcaaccgcta tattaagtgg tttaatgagt
      541 gctaccgata aagcaagatt agatgcctta cacgcattat tgggcgttga aagtgatgcc
      601 gatgtcgttg ttaatacgat taacgaagta ttagcgattt tcaataacta tcaagaaggt
      661 gctgatttag taaatgcttt aagtggcaag gtcgataaag ttgcgggcaa aggtttatct
      721 gctaatgatt ttaccgatat attaaaagcc aagttagata gtttattaag tggcagtaat
      781 tattatgata aaacatatat tgatgctctc aaagacctca atggttggtc gagtgcttta
      841 ttaaccgaaa ccgcattaac taatggtggt gtgattacca aagcaacatt gagcgattat
      901 gatgtcataa agttattcgt tattaacact gcaacgggag agattgatac cgatagtttc
      961 gatacttcca ttggtttagt cgataactat aagtatgtgt tttttgataa tgacggtatt
     1021 tatttatcaa tcgctaatcc aaattgcaca tttaccgata acattacggg ttatcaatta
     1081 aagattatcg gtcaaaaata tgaagcccaa gatgccgaaa atgtcaaata tgataggacc
     1141 gttaaaaatc tattagaagc cgaaaat
//
LOCUS       DAOSEX010000303         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_129937_length_1165_cov_2.097297, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000303 DAOSEX010000000
VERSION     DAOSEX010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129937_length_1165_cov_2.097297"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="PK612_04915"
     CDS             complement(<1..729)
                     /locus_tag="PK612_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH95481.1"
                     /translation="MDIILQTNNLSKRFKNKLALKNVNMTIYRGDIYGFVGENGAGKS
                     TLIRLVTNILRPSTGSYSLNLPKRLGVVGAIVESPAFHPGLNALQNLRYQSDLLGYKK
                     TDEELLVLLDKVGLEEQRNSKKVVKNFSLGMKQRLSIAVTLLSNPEFILLDEPMNGLD
                     PAGIKEMRDLILELNRKGTTFLISTHILTELDKVATRYGFISHGELVEEISVRDLHKK
                     AKSYTEVKLGSEVNAKVRELLSGLN"
     gene            complement(867..>1165)
                     /locus_tag="PK612_04920"
     CDS             complement(867..>1165)
                     /locus_tag="PK612_04920"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOH95482.1"
                     /translation="PPKRGFFAYTPLGQERVLETILDAAVEEQITGLCYFEPGWINVN
                     GLTVATIEGIKYLKEKNIKAGSTLFNEALFSRSGRPNPALSVKNNMDAFKKSDN"
BASE COUNT          347 a          250 c          172 g          396 t
ORIGIN      
        1 atttaaaccg ctaagtaatt cacgcacttt agcgtttacc tcactaccta atttaacttc
       61 ggtataactc ttagcttttt tatgcaagtc acgcacggaa atttcttcga ctaattcacc
      121 gtgactaata aacccgtaac gcgtcgctac tttatctaac tcagttaaaa tgtgagtaga
      181 aattaaaaat gtcgtacctt ttcgatttaa ttctaaaatt aagtcacgca tctctttaat
      241 gcccgcgggg tcaagcccgt tcattggttc atcaagcaaa ataaattcag gattacttaa
      301 aagggtcacg gcgatactta accgttgttt catccctaaa ctaaagtttt taacgacttt
      361 tttactattg cgttgttctt ctaacccaac tttatcgagt aacactaata actcttcatc
      421 agtcttttta taacctaata aatcactttg atagcgtaaa ttttgtaaag cgtttaagcc
      481 gggatggaaa gcaggacttt cgacaatcgc tcccactacg ccaagtcttt ttggtaagtt
      541 taatgaataa ctcccagtcg aaggccgtaa aatattcgtc actaggcgaa tcagtgttga
      601 tttaccagcg ccgttttcac caacaaaccc gtaaatatca ccgcgatata tcgtcatatt
      661 tacatttttt agcgctagtt tatttttaaa ccgtttggag agattattcg tttgtaagat
      721 aatatccata aatgcacttc tctactttca cttttaatta cttagtaatt atacactact
      781 taacactaaa cgaaaaactc gtaagcgtgt tagtaaactt ttctttacaa atgaaaagcc
      841 ctcgctactt gcgaggactc tcccttttaa ttatcactct ttttaaaagc atccatatta
      901 tttttaacgc taagtgctgg attaggtcgg ccgcttcttg aaaacaaagc ttcattaaat
      961 aatgtactgc cggccttgat atttttttct tttaagtatt taataccttc tattgttgcc
     1021 acagttaaac cattaacatt aatccatcct ggttcaaaat aacataaccc tgtaatttgc
     1081 tcctcaaccg ctgcatctaa aattgtttca aggacacgct cttgtcctaa cggtgtgtag
     1141 gcaaagaaac ctcttttcgg tggga
//
LOCUS       DAOSEX010000304         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_130824_length_1160_cov_1.642534, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000304 DAOSEX010000000
VERSION     DAOSEX010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130824_length_1160_cov_1.642534"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PK612_04925"
     CDS             <1..612
                     /locus_tag="PK612_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH95483.1"
                     /translation="IVCGVNTVVNLFLNALAGYAFARLKFKGREITFRIIILSIMIPG
                     TIMTIPNLIICRLLKIDDTLLVLILPFVMSVYNVFLMRQQFYNLPDELEEAAILDGAG
                     PLKIFFKISLPLVTPMMVVLGITTFMWNYNNFLWTLVAIQSPENFTLARSLGDLVSAG
                     SSNPSMYPIMLAGSVLVSLPLVIIFFALQKYIVGGLSSGAVKG"
     gene            624..>1160
                     /locus_tag="PK612_04930"
     CDS             624..>1160
                     /locus_tag="PK612_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95484.1"
                     /translation="MHKNRKLLWGMMIVALLLSGCKNNRRTSSSSSSGIVAELSNYAE
                     LYETTGTKAKMLSRENDLEWVPYEITGNNEIHVYTDTDKEELTGFGFAMTHSTAYWLQ
                     NMSPALKQQALEDLLLPSGANLNIIRIPLGTSDFTYTNDFYTFNDTLGFSDYDLNGFS
                     IERDLEYLIPSLKDALLIK"
BASE COUNT          343 a          197 c          242 g          378 t
ORIGIN      
        1 attgtctgtg gtgttaatac tgtcgttaat ttatttttaa atgcgcttgc gggttatgcg
       61 tttgcccgcc ttaaatttaa ggggcgtgaa ataaccttta gaattatcat tttatcaatt
      121 atgattccag ggacaataat gacgattcca aatttaatta tttgtcggtt acttaaaatt
      181 gatgacacat tgcttgtgct tattttaccg tttgttatga gtgtgtacaa tgtgttttta
      241 atgcgtcaac aattttataa tttaccagat gaattagaag aagcagcaat tcttgatggc
      301 gcgggaccac ttaaaatttt ctttaagatt agtctcccgc ttgtcactcc aatgatggtt
      361 gtgttaggga ttacgacatt tatgtggaac tataacaact ttctttggac attagtcgcg
      421 atccagtccc cagagaactt cacattagca aggtctcttg gggacctagt gagcgcaggt
      481 agctctaatc catccatgta tccaattatg ctagcgggtt cagtacttgt ctcactaccg
      541 cttgtcatta ttttctttgc attacaaaaa tatatcgttg gtggtctcag tagcggggcc
      601 gtaaaggggt aggggaggta aatatgcata aaaaccgaaa actcttatgg ggaatgatga
      661 ttgtagcctt attactttca ggctgtaaaa ataacagaag aactagtagt agcagtagta
      721 gcggcattgt tgctgaactt agtaactacg ctgaactata tgaaacaaca ggcactaaag
      781 ccaaaatgct tagtcgtgaa aatgatttag agtgggtgcc ttacgaaata actggtaata
      841 atgagattca tgtctacacc gacacggata aagaagagtt aacagggttt gggtttgcaa
      901 tgacgcattc tacggcctat tggctgcaaa acatgagtcc agcgcttaaa caacaagcac
      961 ttgaagattt actcttacca agtggcgcga atttaaatat tattcggatt ccgcttggca
     1021 caagtgactt tacatatacg aatgattttt atacgtttaa cgatacgctt ggctttagtg
     1081 attatgattt aaacggtttt agtattgaac gcgatttaga atatttaatc ccgtcactaa
     1141 aagatgcttt acttattaag
//
LOCUS       DAOSEX010000305         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_133421_length_1145_cov_2.222018, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000305 DAOSEX010000000
VERSION     DAOSEX010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133421_length_1145_cov_2.222018"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PK612_04935"
     CDS             complement(<1..813)
                     /locus_tag="PK612_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014195700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH95485.1"
                     /translation="MDYDVIIVGAGPAGYFCAYELTEKRPNLKVLLIEKGRAINTRSC
                     PILKGLIDKCPINKEGFQECYPSCSITCGFGGAGAYSDGKFNITSEFGGWMQDYLERE
                     ELLYLINYVDSINLKFGASNEITDPYTNKVKEIELRAMSVGVRLLRSKVRHLGTEENF
                     KILGRIFKHLQKKVTMLFETDVVEIITENNEIRGVVTKDNEQFTAPKVLLAVGREGSK
                     WLDTILEKRGIKMDQTQVDIGVRVECNDLIMEEINKHLYEGKFVYRTSTGNTV"
BASE COUNT          362 a          221 c          175 g          387 t
ORIGIN      
        1 gactgtatta ccagtagaag tacgatagac aaatttacct tcataaagat gtttattaat
       61 ttcttccata attaagtcat tacattcgac gcggacaccg atatcaactt gggtttgatc
      121 cattttgatg ccgcgctttt ctaaaatagt gtcgagccat ttactacctt ctcgtccaac
      181 cgcgagtaat actttagggg cagtaaattg ctcgttgtct tttgtaacta caccacgaat
      241 ctcattattt tcagtgatta tttccacaac atcagtttca aataacattg taactttctt
      301 ttgtaaatgt ttaaaaatac ggcctaatat tttaaagttt tcttcagtgc ctaagtgtct
      361 tactttacta cggagtaagc gaacacccac actcatcgca cggagttcaa tttctttaac
      421 tttgttcgta tagggatcag tgatttcatt actagcgcca aattttaaat taatactatc
      481 aacatagttg ataaggtaga gcaattcttc tctttctaaa taatcttgca tccaaccacc
      541 aaattcacta gtgatattaa atttgccatc agaataggct ccagcaccac caaagccaca
      601 agtgattgag caagacggat aacattcttg aaagccttct ttattaatcg gacatttatc
      661 aattaatcct tttaaaattg gacatgagcg agtattaatg gctcgtccct tctcaattaa
      721 taaaactttg aggtttggac gtttttcagt taattcataa gcacagaaat aacctgctgg
      781 tccagcaccg acaataatca catcataatc catagtaaaa gcgtctcctt aatttatatt
      841 attttacctt gaggggtatc tagaaattta ttttccctct ttattttaat aaataaactt
      901 atattgttag ttgaatttaa taatattggt aacgctttat tacatttata aaaaagtccg
      961 actatgatgc ggactaattt acttatctgt gtaaggtaag caatggtgat acaatgcacc
     1021 ctctatagac gaaggtgcac cctctaacca ttatttattg gtatatacac acttagtgac
     1081 acgtgactgg cgtgacagat gtgacaaatg aatagaggct cgaaatgaac ccctaattca
     1141 atatt
//
LOCUS       DAOSEX010000306         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_133721_length_1143_cov_3.781250, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000306 DAOSEX010000000
VERSION     DAOSEX010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133721_length_1143_cov_3.781250"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>841
                     /locus_tag="PK612_04940"
     CDS             <1..>841
                     /locus_tag="PK612_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95486.1"
                     /translation="VVQVEKARKLTPRAVKYMAQNADHVSEVLDDGRVIPKKILNVYV
                     DDDLKIYENRFIMTLINRLQIFIELRHKYIREHGDTRNSDQITIKREVQVEDSLFTVD
                     ATITMSVPSDDEGARESNMDLLERLENIRRRTQFLVNSRFMREMAKAVPVSDPIAQTN
                     IIRLNWAYQDAFKLWNFIHRYEELGISYTTTQAKIEFDKKYLDKINQFSLNSFFTIET
                     EHARLALSNVEQKNIRPIIEAGDLNYELSDERFAKPGLPMRVDARVETAEQEAARLKR
                     EATR"
     assembly_gap    842..851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <852..>1143
                     /locus_tag="PK612_04945"
     CDS             <852..>1143
                     /locus_tag="PK612_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95487.1"
                     /translation="KARLKREEDKRKQKEREALKKARAKERALKRKLEAAERAKRRKE
                     EAKQRQIEKNMERARLRAEKERLLAERKAYQKLMMTEAERLRRARKAVAAQAT"
BASE COUNT          407 a          199 c          245 g          282 t
ORIGIN      
        1 gtcgtgcaag tagaaaaagc gcggaaactt accccacgag cagtcaagta tatggcgcaa
       61 aatgctgatc atgtatctga ggtattagat gatggtcgtg ttatccccaa aaagattctt
      121 aacgtttatg ttgatgacga cttaaagatt tatgagaacc gtttcattat gacactaatt
      181 aatcggttac aaatctttat tgaattgcgg cataaatata ttagagaaca tggtgatacg
      241 cgtaatagtg accaaattac gattaagcgt gaagtacaag ttgaagattc gctcttcaca
      301 gttgatgcaa caattacaat gtcagtgcct agtgatgacg aaggggcacg cgaaagtaat
      361 atggatttac ttgaacgttt agaaaatatt agaagacgga cacaattctt agttaatagc
      421 cgctttatgc gtgaaatggc taaagccgta cctgtgtctg acccaattgc acaaacaaat
      481 attattcgtt taaactgggc gtatcaagat gcttttaaat tatggaactt tattcatcgc
      541 tatgaagaac taggtatttc atatacaaca acccaagcca aaattgaatt tgataaaaaa
      601 tatttagata agattaatca attttcatta aattcattct ttacaattga aacagagcat
      661 gcgcgccttg ctttaagtaa tgttgagcaa aagaatattc gtccgattat tgaagcgggc
      721 gacttaaatt atgaattaag tgatgaacgt tttgctaaac caggattacc aatgcgtgtt
      781 gacgctcgtg ttgaaactgc tgaacaagaa gcagcgcgac taaaacgtga agctactcgc
      841 gnnnnnnnnn naaaagcacg gcttaaacgt gaagaagata aacgtaaaca aaaagaacgt
      901 gaagccttaa agaaagcgcg ggcaaaagaa agagcgctta aacgcaagtt agaagctgct
      961 gaacgcgcta aacggcgcaa agaagaagca aaacaacggc aaattgaaaa gaatatggaa
     1021 cgggcacgct tacgcgcgga gaaagaacgc ttacttgccg aacggaaagc atatcaaaaa
     1081 ttaatgatga cggaagcaga acgcttacgc cgagcacgta aggctgttgc cgcacaagca
     1141 act
//
LOCUS       DAOSEX010000307         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_135198_length_1135_cov_3.144444, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000307 DAOSEX010000000
VERSION     DAOSEX010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135198_length_1135_cov_3.144444"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /gene="fusA"
                     /locus_tag="PK612_04950"
     CDS             <1..1056
                     /gene="fusA"
                     /locus_tag="PK612_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH95488.1"
                     /translation="NSTMRASQMFSLLGAKQNNIQKAIAGDIVAFAKLDGITTGHTLC
                     DKNDVVTFPRANYPTIVYRRAISAKNSRDEEKLVQALYRVAAEDPTLEITRVPETKQL
                     LLGGLSDSHLNFVLDKVKTTFGFEANTDAPQVIYRETVKREASAVGRYVKQSGGSGFY
                     GVVEMRFGPSGSDENVFTEEIFGGTVPKNYHPAVEKGFFESVQEGQLAGFPVIGMKAT
                     LFDGKYHSVDSNEQAFRMAAILAYREAYMNCAPTLLEPIMKITISVTNEFTGNVMNDL
                     NQRRARVLSMDESGYGTQEITALVPEAELLDYAIKLRVLSQGSGSFNSEFDSYQEVPT
                     NLLQGVIAANSRLEKDK"
BASE COUNT          349 a          223 c          249 g          314 t
ORIGIN      
        1 aattccacga tgcgtgcttc acaaatgttt agtcttttag gcgctaaaca aaataacatt
       61 caaaaagcga ttgctggtga tatcgttgct tttgcaaagt tagacgggat taccactggt
      121 cacacattat gtgataaaaa tgatgtcgtt acattcccgc gcgctaatta tccgacgatt
      181 gtttatcgcc gcgctatcag tgcgaaaaat agtcgtgacg aagaaaagtt agtgcaagca
      241 ctctaccgtg ttgccgcaga agacccaacc cttgaaatta cgcgtgttcc agaaacaaaa
      301 caactcttac ttggtggact tagtgatagt catcttaact ttgtgcttga taaagtaaaa
      361 actacatttg gctttgaagc aaataccgat gcaccacaag taatttaccg tgaaacggta
      421 aaacgcgaag ctagtgcagt tggccgttat gttaagcaat caggcggcag cggcttctac
      481 ggagttgttg aaatgcgctt tggcccaagt ggtagtgatg aaaatgtctt taccgaagaa
      541 atcttcgggg gaacagtgcc aaagaactat caccctgctg tcgaaaaagg attctttgaa
      601 agtgtgcaag aaggtcaact tgcgggcttc cccgtaatcg ggatgaaagc aacattattt
      661 gacggtaaat accacagcgt tgatagtaac gaacaagcgt tccgcatggc cgctatttta
      721 gcctatcgcg aagcttatat gaattgtgcc ccgactttac ttgaaccgat tatgaaaatt
      781 accatcagtg tcacaaatga atttacgggt aacgttatga atgaccttaa ccaacgtcgg
      841 gcacgggtat taagtatgga tgaaagtggt tatggtaccc aagaaattac cgccttagtg
      901 cctgaagcag aactattaga ctacgccatt aaacttcggg tcttaagcca aggcagtggt
      961 agtttcaata gtgaatttga tagctatcaa gaagtaccca caaacttatt acaaggtgtt
     1021 atcgctgcca attcacggct tgaaaaagat aaataattag atagtttaca taaagaaaac
     1081 catcggtgtg cgccgatggt tttttgtata taatcgcttt attttgtagt ttcta
//
LOCUS       DAOSEX010000308         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_136495_length_1128_cov_2.886300, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000308 DAOSEX010000000
VERSION     DAOSEX010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136495_length_1128_cov_2.886300"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="PK612_04955"
     CDS             <1..1023
                     /locus_tag="PK612_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH95489.1"
                     /translation="VPSLCPGRAIQVFRTLSIIIGSVLIFSQLLLVFLCKERDRSEVD
                     SEEIIKVKDIFKVVFQNKPLLVMLGAIVLHYLGGSMFNNLGQNYMHFTFGYAEGGQAF
                     FYFLVGYAVGSIAANFIFGFLVKKYTRAQILRGAFTISAIGNSLLFVIGSTHAFGWLQ
                     IPNSIYQWILPVLALIIFTPNGVFYLTLLVQITNTVEYNEWKFGERKEGVVFAARPFA
                     AKISNAITVIVVFLTLIVGGVYDITNKISDYEVLGGLPEGDARRLITEEVQDLAQAAI
                     DGANLGRARFVLILGMALLPLICQTLAYLLVSRKYPIDEKMYDQMVKEIDERRETAAE
                     EQVVEA"
BASE COUNT          319 a          206 c          233 g          370 t
ORIGIN      
        1 gttccaagtt tatgcccagg acgcgcgatt caagtattta gaacacttag tattattatt
       61 ggtagtgtat taattttctc ccaattatta cttgtgttct tatgtaaaga gcgagatcgt
      121 agtgaagttg atagcgaaga aattattaaa gttaaagaca tctttaaagt tgtcttccaa
      181 aataaaccgc ttttagtaat gcttggggca attgtccttc attacttagg tggttcaatg
      241 tttaataacc ttggtcaaaa ctatatgcac tttacttttg gctacgctga agggggccaa
      301 gctttctttt atttcttagt ggggtatgct gttgggtcaa ttgccgcgaa ctttattttt
      361 ggcttccttg tgaaaaaata tacaagagca caaatcttac gcggcgcctt cactattagc
      421 gcaattggta actcactgct ctttgtgatt ggtagtacac atgcttttgg ttggttacaa
      481 attccaaata gcatctacca atggatctta cctgttttag cgctcattat ttttacgcca
      541 aacggtgtgt tttatcttac actgctcgtg caaattacaa atacggttga atataatgaa
      601 tggaaatttg gcgaacggaa agaaggtgtt gtctttgccg cgcgtccttt tgccgcgaag
      661 attagtaacg caatcaccgt tattgttgtg tttttaacac tcattgtcgg cggtgtttac
      721 gatattacta ataaaattag tgattacgaa gtcttaggcg ggttaccaga aggagacgca
      781 cggcgactta tcactgaaga agtgcaagac ctcgcccaag ccgcaattga cggcgcaaat
      841 ttaggcagag cacgctttgt gttaatctta gggatggcac ttttaccact tatttgtcaa
      901 acacttgcct atctccttgt cagtcgtaaa tacccaattg atgaaaagat gtacgaccaa
      961 atggtcaaag aaatcgacga acggcgtgaa accgcagcgg aagaacaagt cgttgaagca
     1021 taagcgttaa ttagaattac cgaaaagtcc aagttatcaa gacttgggct tttttgttaa
     1081 aaattcaatc tttcaccata aattgttagt tattttgtac ttttttgt
//
LOCUS       DAOSEX010000309         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_136666_length_1127_cov_3.048507, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000309 DAOSEX010000000
VERSION     DAOSEX010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136666_length_1127_cov_3.048507"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1127
                     /locus_tag="PK612_04960"
     CDS             <1..>1127
                     /locus_tag="PK612_04960"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95490.1"
                     /translation="ATRVYNAVYNDYAECFDNTDLDYDKVKNRIVEITDDDKVRLGSN
                     NSIRVVGVVSDSYGHMLGGTEEEIENGKKIPVGIAGKLYVDSKDIVDINNIGKFVGSD
                     DEEYGGDVIFQPISAKKGTGIDELLDAILVTAEMLELEANPKRYAYGTVLEARLERGE
                     GPKAMLLIQNGTLTNRDFVVAGAAYGKVRRMTNEHNKALKSAGPATPVAIIGLDEVPE
                     AGDNFIAFETEKEAKELAIKRKQIRIEESRGSSEGMKLSDLHKQIAEGETVVVNVIIK
                     ADNSGSAEAVRGSLEKINVEGVRLNIIRASAGQITESDVLLASASNAVIFGFNIRPNN
                     QVRDLAERLKIEIHLHRVIYALLEQTEQALKGLLKPEFEEKV"
BASE COUNT          390 a          163 c          258 g          316 t
ORIGIN      
        1 gcaaccagag tttataatgc agtttataat gactatgcag aatgttttga taatactgat
       61 ttagattatg ataaagtaaa aaatagaatt gttgaaataa cagatgatga caaagttaga
      121 ttaggtagta ataattcaat tagagtagtt ggtgttgtat cagattctta tggtcatatg
      181 ctaggtggta ctgaagaaga aattgagaat ggaaaaaaga taccagtagg aatagcagga
      241 aaattatatg ttgattccaa agatattgta gatataaata atataggtaa atttgttggt
      301 tctgatgatg aagaatacgg aggtgacgtt atttttcaac caatttctgc gaaaaaagga
      361 acgggtattg atgaactact tgacgcgatt cttgtcactg ctgaaatgct tgaattagag
      421 gctaatccaa agcgctatgc ctacggcaca gttttagaag cgcgtcttga acgcggtgaa
      481 ggaccaaaag ctatgctttt aatacaaaac ggcaccttaa caaatcgtga ttttgtcgtt
      541 gccggagcag cttatggtaa agtacggcgg atgactaatg agcataataa ggcattaaaa
      601 agtgctggac cagcgacacc agtcgccatt attggtcttg acgaagtacc tgaagcgggt
      661 gacaatttca tcgcttttga aacagaaaaa gaagcaaaag aactcgctat taaacgcaaa
      721 caaattcgca ttgaagagag ccgcggtagt agtgaaggga tgaaacttag tgatctccat
      781 aagcaaattg ccgaaggtga aactgttgtc gttaatgtga ttattaaggc tgataatagc
      841 ggtagtgcgg aagcagtacg cgggagtctt gaaaagatta atgtcgaagg tgtgcgcctt
      901 aatattattc gtgcaagtgc tggccaaatt accgaaagtg atgtcttact tgcaagtgcg
      961 agtaatgcag ttattttcgg gtttaatatt cgtccaaata accaagtgcg tgaccttgca
     1021 gaacgactaa aaattgaaat tcatttacat cgcgtaattt atgctttact tgaacaaact
     1081 gaacaagcac taaaaggttt attaaaacct gaatttgaag aaaaagt
//
LOCUS       DAOSEX010000310         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_137414_length_1123_cov_2.927903, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000310 DAOSEX010000000
VERSION     DAOSEX010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137414_length_1123_cov_2.927903"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PK612_04965"
     CDS             <1..925
                     /locus_tag="PK612_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOH95491.1"
                     /translation="EEGTELIGRLKAFLDGDKDVKLPLFTSCSPGWIRYIERYAPEYL
                     GHVSSCKSPQGMSGAIIKNYYAQKLGIEREKIKVVSIMPCIAKKYEASRPEMEVDGVR
                     DIDLVLTTRELARFIKRHVIDFDMLTNKQLTSPLASYTGAGVIFGVTGGVMEAALRTI
                     KALLEKEDAYLIDIREVRGFESIKEATLTIDGKELNFAVVHGAKHFKEVLELVKSGKK
                     QYAFIEFMGCHGGCIFGGGQPITKAKDFEKIDVLKERAKALYRIDQSGKIRRSHENPH
                     VLKLYEEFLGHPGSHLAHKLLHTTYSKKDVY"
     gene            926..>1123
                     /locus_tag="PK612_04970"
     CDS             926..>1123
                     /locus_tag="PK612_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95492.1"
                     /translation="MKKKIATLLLTSLLLGCQKQTVKLSLLVPAGSPLLTVAGVTSEL
                     EKEVTVGPDLIPAAFTKGEKDL"
BASE COUNT          375 a          181 c          239 g          328 t
ORIGIN      
        1 ggaagaaggc actgaattaa ttgggcgttt aaaagcattc cttgacggtg ataaagatgt
       61 gaagttacca ctttttacaa gctgttcacc aggctggatt cgttatattg aacgctatgc
      121 tcctgaatac ttaggacatg tttcttcatg taaatcacca caaggcatga gcggagcgat
      181 tattaaaaat tattatgcgc aaaaattagg gatagaacgc gaaaagatta aagtcgtgtc
      241 aatcatgccg tgtattgcta aaaaatacga agcaagtcgt ccagaaatgg aagttgatgg
      301 tgttcgtgat attgatttag tactcacaac tcgtgaatta gcgcgtttta ttaaacgcca
      361 tgtcattgat tttgatatgt taacaaataa acaattaaca agcccgcttg cctcttacac
      421 tggggctggt gttatttttg gcgtaacagg tggcgttatg gaagcagcat tacgtactat
      481 taaagcatta cttgaaaaag aagatgctta tttaatcgat attcgtgaag tacgcggctt
      541 tgaaagtatt aaagaagcaa ccttaacaat tgatggtaaa gaacttaact ttgcggttgt
      601 gcacggcgct aaacatttta aagaagtgct tgaactagta aaaagtggta aaaagcaata
      661 tgcctttatc gaatttatgg ggtgccatgg cggctgtatc tttggtggag gtcaaccgat
      721 tactaaagct aaagactttg aaaagattga tgtattgaaa gagcgcgcta aagcactcta
      781 ccgcatcgac caaagtggaa aaatccgccg ttcacatgaa aatccacatg tcttaaaatt
      841 atatgaagaa tttttaggcc atcctggctc acatttagcg cataaactat tacatacaac
      901 atatagtaaa aaggatgtgt attaaatgaa aaagaaaatt gcgactttgt tattaactag
      961 cttactttta ggttgtcaaa aacagactgt taaattatct ttattagtgc ctgctggcag
     1021 cccgttatta acagttgcag gtgtaacaag tgaattagaa aaagaagtga cagttggacc
     1081 cgacttaatt ccggcagcgt ttactaaagg tgagaaagat tta
//
LOCUS       DAOSEX010000311         1123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_137468_length_1123_cov_2.244382, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000311 DAOSEX010000000
VERSION     DAOSEX010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137468_length_1123_cov_2.244382"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PK612_04975"
     CDS             complement(<1..749)
                     /locus_tag="PK612_04975"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOH95493.1"
                     /translation="MNQDFIYGADISSLLAVEELGGTFTNDNGEKEDFFKILKSNGFN
                     LARIRLFVDPKTNKGVSYGAGHNDLERALELAKRAKAHDFKVLLNIHYSDFWADENHQ
                     TLPKAWAKLDFADLEYQVYAYTKEVVTTFKEENITLDYIQVGNEVNKGILWPHGKLFR
                     RKKATQTEYDALAILLNAGIKAIREVTPNVKIMIHLGQGSNPEMYADFLHFMEPRLFK
                     FDAIGLSYYPYIHRQITELQATLLFIKTYYP"
BASE COUNT          334 a          216 c          160 g          413 t
ORIGIN      
        1 taaggataat aagttttaat aaataaaagt gtcgcttgta attcagtaat ttggcggtga
       61 atatacggat aataagataa tccaatagca tcaaatttaa aaagccttgg ttccataaaa
      121 tgtaagaaat ctgcgtacat ctctggatta gaaccttgcc ctaaatgaat cattattttc
      181 acattcggtg ttacttcacg aattgcttta atcccagcat taagtaaaat cgctaaagcg
      241 tcatattctg tttgtgttgc ctttttacga cggaaaagct taccatgcgg ccataagatt
      301 cctttattaa cttcgttacc aacttgaata taatcaagtg ttatattttc ttctttaaaa
      361 gtagtaacaa cttctttagt gtaggcatag acttgatatt caagatccgc aaagtctagt
      421 ttagcccatg cttttggtaa tgtttgatga ttttcgtctg cccaaaagtc actataatga
      481 atattaagta atactttaaa gtcgtgtgct tttgctcttt ttgctaattc tagcgccctt
      541 tctaaatcat tatgaccagc gccataagac acgcctttat tcgtcttagg atcaacaaag
      601 agacgaatac gcgctaaatt aaagccgtta cttttaagta ttttaaagaa atcttctttt
      661 tcaccgttat cattagtgaa agtaccgcct aactcttcca ctgctaaaag tgaagagata
      721 tccgcaccat aaatgaaatc ttgattcatt atcttccctc ctaaaactaa gcaatacaac
      781 actataatta taaatgacta atgcttaact ttcattaaaa gtcacaacat agtaataaaa
      841 aaaacgacac taacaagtag tgtcgcccac cttgagctat tatttggttc ctgcctagtg
      901 ccgcccaagg tgtattttta tagtagcata taaatttaat aaaagtaaaa ctaatactct
      961 tttggttctg cttcaggttc tgctttttct gtttcttttt caacctttac tttcggttct
     1021 tttacttctg tcttttttgg agcagcttct actttttctg cttcttttgg tttttcttct
     1081 tcacttttaa cttctacttc aacctcttgc actgtttctt ttt
//
LOCUS       DAOSEX010000312         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_138342_length_1118_cov_3.817498, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000312 DAOSEX010000000
VERSION     DAOSEX010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138342_length_1118_cov_3.817498"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PK612_04980"
     CDS             <1..970
                     /locus_tag="PK612_04980"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOH95494.1"
                     /translation="LKDALLTKRVFARVSPENKVQIVNALQANGEIVAMTGDGVNDAP
                     SLKTADIGIAMGITGTDVAKGAADMVLTDDNFASIEKAVQEGRGIYANIRKTVWFLLS
                     SNFGEVITMLVAVLVGYPFPLSALHILWVNLITDGVPALALGADSKDPDIMKEKPRDK
                     KESLFANGGYFITIFFGSVIALITTLAFLFIPVVQHGMRSVQDINTFFAENPELKVRA
                     MTYAFTALGVSQLFHMFGMSNLNKTFFRTFTKKKWFLWVAFFIGFLLQIAVTEIHALT
                     VAFGTVELTFIEWVFLALLSTTPLVIHELRVFFRFIREKIKNRKSH"
BASE COUNT          334 a          178 c          224 g          382 t
ORIGIN      
        1 cttaaaagat gctttgctta ctaagcgtgt ctttgcccgt gtatctcccg aaaacaaagt
       61 acaaattgtt aatgccttac aggctaatgg tgaaattgta gcgatgacgg gggacggtgt
      121 taatgacgct cctagtttaa aaactgctga tatcggaatt gcgatgggta ttaccggaac
      181 tgatgtggct aaaggtgccg ctgatatggt attaactgat gataactttg cgagtattga
      241 aaaagcggtg caagaaggac gcggtatata cgctaatatt cgtaaaacag tgtggttttt
      301 attatcaagt aactttggtg aagttattac gatgcttgtt gccgtcttag taggttatcc
      361 tttcccgctt tcggcattac atattttatg ggttaattta attactgatg gtgtaccggc
      421 gcttgcactt ggcgctgact cgaaagaccc agacattatg aaagaaaaac cgcgtgataa
      481 gaaagaatca ttattcgcta atggtggtta ctttattacg atattcttcg gaagtgttat
      541 tgctttaatt actactttgg cgttcttatt tatcccggta gtgcaacacg ggatgcgtag
      601 tgttcaagat attaatactt tctttgcgga aaatcctgaa cttaaagtta gagcaatgac
      661 ttatgctttt actgcattag gtgttagcca actcttccat atgtttggca tgtcaaattt
      721 aaataaaaca ttctttagaa catttacgaa gaaaaagtgg ttcttatggg ttgctttctt
      781 tattggcttc cttttacaaa ttgcggtaac tgaaattcat gctttaactg tggcgtttgg
      841 tacagttgaa ttaacattta ttgagtgggt atttttagca ttacttagta caacaccact
      901 tgtaattcat gaattaagag ttttcttccg ctttatccgt gagaaaatca aaaatcgaaa
      961 aagtcattaa taacactatt gctttatgac attagtaaag ttataataaa tttccttgtg
     1021 aggataagta tatgaaaaaa cgaaatattt ttactaaatt ttctttaact gcaattttac
     1081 tactagcgcg tacactacta aaagcaaaga gcgcagat
//
LOCUS       DAOSEX010000313         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_138889_length_1115_cov_3.150000, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000313 DAOSEX010000000
VERSION     DAOSEX010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138889_length_1115_cov_3.150000"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..376
                     /gene="infA"
                     /locus_tag="PK612_04985"
     CDS             158..376
                     /gene="infA"
                     /locus_tag="PK612_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOH95495.1"
                     /translation="MAKQDLIVQDGIITEALSNAMFRVRLENGHEVISHISGKMRMNY
                     IKILPGDKVQLEMSPYDLNKGRITFRYK"
     gene            385..501
                     /gene="ykgO"
                     /locus_tag="PK612_04990"
     CDS             385..501
                     /gene="ykgO"
                     /locus_tag="PK612_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOH95496.1"
                     /translation="MKVRASVKKRSADCKIVRRKGVVYVINKKNPKFKQRQG"
     gene            527..904
                     /gene="rpsM"
                     /locus_tag="PK612_04995"
     CDS             527..904
                     /gene="rpsM"
                     /locus_tag="PK612_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOH95497.1"
                     /translation="MARISGVDLNKNKRGEIALTYIYGIGKVTAKKILTANNIDVNTK
                     VKDWTDENVNGIVRYLADNLKLEGELRGETQQNIKRLQDIGCYRGIRHRKGLPVRGQS
                     SKTNARTRKGKRKTVANKKMVTK"
     gene            955..>1115
                     /locus_tag="PK612_05000"
     CDS             955..>1115
                     /locus_tag="PK612_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOH95498.1"
                     /translation="MAKQQGASSAKAKAKKRIVKVESEGKVFLNATFNNLIVTFTNLS
                     GQAISWSSAG"
BASE COUNT          429 a          163 c          241 g          282 t
ORIGIN      
        1 attaggacaa aagtgttaat gggaatggtc atttatgggg aaattccacc cttttcgttc
       61 cgtttctttt gattcgtata attaattgta taaaaaaatt ttggagtgtg tggataagtt
      121 atatatttgc gttccctttt aaaaaggggg atattgcatg gctaaacagg atttgattgt
      181 acaggacgga attataacag aagcgttaag taacgcgatg ttcagagtac gattagaaaa
      241 tggacacgaa gtaatatcgc acatatcggg taaaatgcgt atgaattata tcaaaatttt
      301 accgggcgat aaggtgcagt tagagatgag tccgtacgat ttaaataagg gaagaatcac
      361 ttttagatac aaataataaa gaccatgaag gtaagagcat cagttaagaa aagaagcgca
      421 gactgcaaaa tcgttcgcag aaaaggcgtg gtatatgtaa tcaacaagaa gaaccctaag
      481 ttcaaacaaa gacaaggtta attaaaacac taaacattta ttagcaatgg caaggatatc
      541 cggtgtagat ttaaataaaa acaaacgtgg cgaaatcgcc cttacttata tatatggtat
      601 aggtaaagta accgcaaaga aaattttaac ggcaaacaac attgacgtga acaccaaagt
      661 aaaagattgg acggacgaaa atgtgaacgg tatcgttaga tatttagcgg acaacctaaa
      721 actagaagga gagcttcgtg gagaaacaca acaaaatatc aagcgtttgc aagatatcgg
      781 ttgctaccgt ggtatccgtc acagaaaagg attgccggtt cgtggtcaaa gttctaaaac
      841 gaatgcaaga acacgtaagg gtaaacgtaa aacagttgct aacaagaaaa tggtaactaa
      901 ataataatta gtcaatgggt ctgtttgcgg accactataa taaacgaata aatcatggca
      961 aaacaacaag gagcaagtag cgcaaaagca aaagcgaaga aaagaatagt taaagttgaa
     1021 tcagaaggta aagtgttttt gaatgctact ttcaacaact taattgtgac tttcactaat
     1081 ctttcaggac aagcaatttc atggtcaagt gccgg
//
LOCUS       DAOSEX010000314         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_139091_length_1114_cov_2.963173, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000314 DAOSEX010000000
VERSION     DAOSEX010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139091_length_1114_cov_2.963173"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..880
                     /locus_tag="PK612_05005"
     CDS             89..880
                     /locus_tag="PK612_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95499.1"
                     /translation="MEKSLQPTEPKKRRRRKRPRRIRQNWFFRFLRNFVKLFKKRPLI
                     YDHNEDIVGPAIFVSNHSGAAGPMDLSMFFPYYFVPWGAHEMRGNYRTRWKYLYYVFY
                     QQKLHYGKFKSFFLATILAVINPFLYANMRLIPTYQDIRFTKSLRESLSYLDDGIPIL
                     VFPEDSSEGYFDTIIKFNEGVVVLADYVDKHRNLNIPIYPVYYSKRAREIEIGKPAFY
                     RDLKAKGHTRSEIADILRKHVNTLYENIKARKARNAKARERVKKG"
     gene            complement(888..>1114)
                     /locus_tag="PK612_05010"
     CDS             complement(888..>1114)
                     /locus_tag="PK612_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95500.1"
                     /translation="VNDGLLFRVTIKTESRQHIGIERESVKNFINMSKLKIGQLLYQL
                     LLKNTIQVIKLKLIKNKAKRRQFSRLLSLY"
BASE COUNT          357 a          193 c          207 g          357 t
ORIGIN      
        1 aacgatatta tagcaaatat gccgcactaa gtttaagatt tggtatcttc ctttgctaaa
       61 agtgtgctta ttcggtaaaa tgaacaatat ggaaaaaagt ttacaaccga ctgagccgaa
      121 aaaaagacgg cgcagaaaaa gaccacgaag aattaggcaa aactggttct tccgtttttt
      181 acgtaatttt gtgaagctat ttaagaagcg tccgcttatt tatgaccata atgaagatat
      241 tgttggccca gctatttttg ttagtaatca tagcggtgca gcaggaccaa tggatctttc
      301 aatgtttttc ccgtattact ttgtaccatg gggcgcgcat gagatgcgcg gcaactatcg
      361 aacacgctgg aaatacttat attatgtttt ctatcaacaa aaattgcatt acggtaaatt
      421 taagagcttt ttcttagcaa cgattctggc ggtaattaat ccgtttcttt acgcaaatat
      481 gcggcttatc ccaacttatc aagatattcg gttcacaaaa agtttacgtg aaagtttaag
      541 ttatttagat gacgggattc ctattcttgt tttccccgaa gattcaagtg aaggctactt
      601 tgacacgatt attaaattta atgaaggtgt tgtcgtgctt gctgactacg tcgataaaca
      661 tcgtaatctt aacattccaa tttatcctgt ttattacagt aaaagagcac gcgagattga
      721 aattggtaaa cctgctttct atcgtgactt aaaagcaaag ggtcacaccc gttcagaaat
      781 agcggatatt ttacggaaac acgttaatac attatacgaa aatattaaag ccagaaaagc
      841 gcgaaatgcc aaagctagag aacgagttaa aaaaggttaa attactctta atataacgac
      901 aaaaggcggc taaattgccg cctttttgct ttgtttttaa ttagtttaag cttgataact
      961 tgaatcgtgt tcttcaacaa caactggtac aataattggc caatttttag cttggacata
     1021 tttataaagt tctttacgga ttcgcgttca ataccgatat gttgccgtga ttctgttttg
     1081 attgtaactc taaagagtaa accatcattt acat
//
LOCUS       DAOSEX010000315         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_139172_length_1114_cov_1.941454, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000315 DAOSEX010000000
VERSION     DAOSEX010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139172_length_1114_cov_1.941454"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PK612_05015"
     CDS             <1..938
                     /locus_tag="PK612_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
                     /protein_id="HOH95501.1"
                     /translation="VLSYKRPLYGRLTFKIKLEDFDYFDASSFYPNYSNEDKVIAYSI
                     FGGIPHYLARISDRLTIKENVINLLSGKNADFKDEIDFFLKQELRSISVYGMIISAIA
                     SGATKLNEIAQSALIKETGTASKYIDTLRDLNIVEKEVCFGEKINSKRTIYKIKDNFF
                     NFYYFFIHKNKSQIALMDDNYFYDAFIVPSLSTYVGNKFETIAKQFLIKKNKMNKVDP
                     FFEIARYWGNNKDLKREVKIDLVTKGKNALVVYECKWTNDLFRLAEVKSFKQNASHLR
                     PTSYQAFSKSSYSDDARKALDKAYIIDDLYSLQIK"
BASE COUNT          392 a          180 c          204 g          338 t
ORIGIN      
        1 atgtcttatc ttataagcga cctttatacg gacgtctaac ttttaagatc aagttagagg
       61 attttgatta ttttgatgcg tcttcttttt atcccaacta tagtaatgaa gataaagtta
      121 ttgcctatag catttttggt ggcatccctc actatcttgc tagaataagt gatcgtttaa
      181 ctataaaaga aaacgtaatt aatttattaa gtggcaaaaa cgctgatttc aaagatgaga
      241 tcgatttttt cctcaaacaa gaactgcggt ctatcagcgt ttacggcatg attatttctg
      301 cgattgccag tggtgcgaca aaattaaacg aaatcgcaca gagtgcttta attaaagaaa
      361 cagggactgc ttcaaaatac atcgacacct tacgcgattt gaatattgta gaaaaggaag
      421 tttgttttgg cgaaaaaata aattcgaaaa gaaccatcta caaaataaag gataattttt
      481 ttaattttta ttactttttt attcacaaaa acaaatcgca gattgccttg atggatgata
      541 actattttta tgacgctttt atcgttccaa gtttaagcac atatgttggt aataaattcg
      601 aaacaattgc gaaacagttt ttaatcaaaa aaaataaaat gaataaagtt gatccatttt
      661 ttgaaatcgc ccgctattgg ggaaataaca aagatttaaa gcgcgaagtt aaaatcgacc
      721 ttgtaactaa aggtaaaaat gccttagttg tgtatgaatg caaatggaca aacgacttgt
      781 ttaggctcgc tgaagtaaaa agtttcaagc aaaatgctag tcatctacga ccaacatcat
      841 atcaagcctt ttccaaatca agttatagcg acgacgcaag aaaagcatta gataaagctt
      901 acataattga tgatctatat tctctgcaaa tcaaataaat tattaaaacc atatagtaaa
      961 tattagtaaa tgggattata tacctggccc accaagatgg tcgcaccgtt ataggagagg
     1021 ctgaaggcaa agggccgatt aaagatgaga ggttcggcac tgtagttatc gccggcagat
     1081 tcgccgatgc tcgtgacggt taccgaggct gaat
//
LOCUS       DAOSEX010000316         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_140706_length_1106_cov_2.297812, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000316 DAOSEX010000000
VERSION     DAOSEX010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140706_length_1106_cov_2.297812"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="PK612_05020"
     CDS             complement(<1..772)
                     /locus_tag="PK612_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOH95502.1"
                     /translation="MEKVIKVIEESFLAPLLSDKNITDISYNGHKLFYFSNESGRKLY
                     GEINHKTVASFLRNVANFANERFSYADTFLDISIGKYRLSAMHYACSRFEFKESLSFA
                     LRINHNFLASNPHYLNNDVVDVLKYVMKENRSIIISGTTGVGKTTLQKYLLMLLPANT
                     RVLVIDNVLELSEVTTLNKDLDLTLWQSLTNKEEEIKSFIQKALRFHPDYLIIAESRG
                     GEMKEILQGAISGHPNIVTLHADSTALAYERVMYVAKHD"
     gene            complement(775..>1106)
                     /locus_tag="PK612_05025"
     CDS             complement(775..>1106)
                     /locus_tag="PK612_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95503.1"
                     /translation="TYSTLLAINIRYHHVNEVLSSVTHDHIYHVTTMDLVTYELYFEK
                     PLVRVVVANHFNLNMKRENYEYTITFYTNGVVSEESERSRYFTINLVCPLGFGVTFDK
                     TFAYYVS"
BASE COUNT          361 a          203 c          165 g          377 t
ORIGIN      
        1 catcatgctt tgctacatac atgacgcgtt cgtatgctaa agctgtacta tcagcgtgga
       61 gtgttacaat atttggatga ccgcttattg ctccttgcag tatttctttc atctcaccac
      121 cccgactctc ggcaataatt aaataatcgg gatgaaaccg taaagccttt tggataaaac
      181 ttttaatttc ttcttcttta tttgttaatg attgccaaag tgttaaatct aaatctttat
      241 ttaatgttgt gacttcactt aattcaagga cattatcaat cactaacacc cgcgtattag
      301 cgggtaaaag cattaataaa tacttttgta aagtcgtttt accgactcca gttgtgccgc
      361 taatgatgat ggagcgattt tctttcatca catactttag aacatcaaca acatcattat
      421 ttaaataatg cggattactt gcgagaaaat tatgattaat gcgcaaggca aaacttaaac
      481 tttctttaaa ttcaaatcta ctacatgcat aatgcatcgc actaaggcga tactttccaa
      541 ttgatatatc aagaaatgta tcagcgtaag aaaatctctc attagcaaaa ttagcgacat
      601 tacgtaaaaa gcttgcgaca gtcttatgat taatttcgcc atataacttg cgaccactct
      661 catttgaaaa gtaaaataat ttgtgaccgt tataactaat atctgttatg tttttatcac
      721 taaggagcgg ggctaaaaaa ctctcttcaa ttactttaat tactttttcc attgttaact
      781 cacataatag gcaaatgttt tatcaaaagt cacaccaaag cctagtggac acacgaggtt
      841 aattgtaaaa tatcgacttc tttcactttc ttcactaacg actccatttg tgtaaaatgt
      901 aatcgtatat tcgtaatttt ctcttttcat attaaggtta aaatgattag cgactacaac
      961 tcttacaagc ggtttttcaa agtagagttc ataagtaact aaatccattg ttgtcacatg
     1021 ataaatatga tcatgagtta cagaactaag tacttcatta acatggtgat agcgaatatt
     1081 tatcgctagc agtgtgctat aagtaa
//
LOCUS       DAOSEX010000317         1106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_140744_length_1106_cov_1.897241, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000317 DAOSEX010000000
VERSION     DAOSEX010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140744_length_1106_cov_1.897241"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1053
                     /gene="xseA"
                     /locus_tag="PK612_05030"
     CDS             151..1053
                     /gene="xseA"
                     /locus_tag="PK612_05030"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOH95504.1"
                     /translation="MGLFDQEKKRPLPKFPKVIGIITPKDSAAQSDLVTNINRRYPLV
                     TLKIVNATFQGESAPSSLISAFEVLQNEHIDVLIIARGGGSSDDLWAFNDESLIMALS
                     AKKVPLISAIGHEIDTTLVDYLADVRASTPTAAAELVVPDQNELRQTLTDYSVRLENA
                     MSTVLIQREKELNHLIIRPVLVNFGAIVENLTTKLLTLSTKLGARSAENIARAEITLH
                     SLKERLNKAITRTYNVINETLVAKSTRLEALNPTNVLNRGYAFISKNEEIIKSAKKLK
                     VKDEVKITFSDGSVLAEVKGGEDE"
BASE COUNT          370 a          194 c          236 g          306 t
ORIGIN      
        1 gctaaaagat ggtgacgaag taattgccac aggtcgtatt acggtttatg aaaaaagtgg
       61 taactaccaa ctcgtggcca ctgaagttag ttattacgga cttggtgcga agttgcttga
      121 acttgctaaa cttaagtaaa gttagacaag atggggcttt ttgatcaaga aaagaagcgc
      181 ccattaccta aatttcccaa agttattggt attattaccc caaaagatag cgcggcacag
      241 agtgatttag tgacgaatat taaccgtcgc tatccgcttg tgactttaaa gattgttaac
      301 gctacttttc aaggggaaag tgcgccttct tcactcatta gtgcctttga agttttacaa
      361 aatgaacata ttgatgtctt aatcattgcc cgtggcggtg gaagtagtga cgacttatgg
      421 gcatttaatg atgaaagtct cattatggcc ttaagtgcaa agaaagtccc acttattagt
      481 gcgataggac atgaaatcga tacaacatta gtcgattatc ttgccgatgt gcgcgctagt
      541 accccaactg ctgccgccga acttgttgtt cctgatcaaa atgaattacg gcaaacgctt
      601 actgattata gtgttcgtct tgaaaacgct atgagtactg tgctcataca acgcgaaaaa
      661 gaactgaatc acttaataat aagaccagta cttgtaaact ttggcgcaat tgtggaaaac
      721 ttaacgacaa agcttttgac actgagcact aaattaggag cccgtagcgc cgaaaacatc
      781 gctcgagcgg aaattacctt gcattcatta aaagaacgct taaataaggc gataactcgt
      841 acgtataatg ttattaatga aacgcttgtc gctaagagta cacgacttga agcacttaat
      901 ccgacgaatg tattaaatag aggctatgct tttattagta agaatgaaga aattattaaa
      961 tcagcgaaga agttaaaagt aaaagacgaa gttaagatta catttagtga cggcagtgta
     1021 ctagccgaag ttaaaggagg agaagatgag taaagaaaaa ttatcatttg aacaaaaact
     1081 cgcacgctta aatgctattg ttagtg
//
LOCUS       DAOSEX010000318         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_140855_length_1105_cov_2.959048, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000318 DAOSEX010000000
VERSION     DAOSEX010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140855_length_1105_cov_2.959048"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1105)
                     /locus_tag="PK612_05035"
     CDS             complement(<1..>1105)
                     /locus_tag="PK612_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95505.1"
                     /translation="QTEINSINAALNTLESTNVEVLFIKRAQKETSEKITKLENEVAL
                     LNANIKRFRFLDIFLSIITLSIYFFVLKSKNNKLRKEIALLNKTIDELNVKLENYAAE
                     LATMTSTESDIKLKQKLDEWTQKVNQKQKELEAFKEHVFDVFRKYSVKVNSVSDVQVA
                     YFLINSHYEKHLALNKKLNDVNFKLKNYDIKKINSDLNSFALLDEEELNRNKSNVDIK
                     NDELIKKITALEKQIEANEDLASNLENYKFERDELRTTMQNYEQKKELLTIARTLLEH
                     ANQQLAARYLTPLQNNVNDLLTKIKLEGYAVMFDGNSEMKLKDLDTNEYYDFAYYSAG
                     SQDLISVLVRVALIDLVYQDLKPFIILDDTFVN"
BASE COUNT          328 a          168 c          156 g          453 t
ORIGIN      
        1 aagttaacga atgtatcatc aagaatgata aatggtttta agtcttgata cactaaatca
       61 attaaggcaa cgcgcactaa gaccgaaata agatcttgac taccagcact gtaataagca
      121 aagtcgtaat attcattcgt gtctaaatct tttaatttca tttcagagtt tccgtcaaac
      181 attacagcgt agccctctaa tttaattttc gtaagtaagt cgttaacatt attttgaagc
      241 ggggttaaat accttgctgc tagttgctga ttagcgtgtt ctaataatgt tctagcgata
      301 gttaataatt cttttttctg ctcatagttt tgcattgtag tgcgtaattc gtcgcgttca
      361 aacttgtaat tttcaaggtt actcgctaag tcttcatttg cttcaatttg tttttctaaa
      421 gctgttattt ttttgattaa ttcatcgttt ttgatgtcga cattactctt attcctgttt
      481 aattcttctt catctagtag ggcgaaagaa tttaaatcac tatttatttt tttaatatca
      541 taattcttaa gtttaaaatt tacatcatta agctttttat taagcgctaa atgtttttca
      601 tagtgactat taataaggaa ataagcaact tgaacatcac taacactatt aactttaacg
      661 gaatacttac gaaatacatc aaaaacatgt tctttaaaag cttctaattc tttttgtttc
      721 tggttaactt tttgtgtcca ctcatcaagt ttttgtttaa gtttaatgtc actttctgtg
      781 ctagtcattg tcgctagttc agcggcataa ttttcaagtt ttacattaag ttcatcaatc
      841 gttttattta aaagcgctat ttcttttctt aacttattat tcttactttt aaggacaaag
      901 aaataaattg atagcgtgat aatgcttagg aaaatatcaa ggaaacgaaa tctttttata
      961 tttgcattaa gtaaggctac ttcattttct aatttagtta ttttttcact agtttctttt
     1021 tgcgctcttt taataaataa gacttcaaca tttgtacttt cgagagtatt tagagcagcg
     1081 tttattgaat taatttctgt ttgtg
//
LOCUS       DAOSEX010000319         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_141138_length_1104_cov_2.034318, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000319 DAOSEX010000000
VERSION     DAOSEX010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141138_length_1104_cov_2.034318"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..537)
                     /locus_tag="PK612_05040"
     CDS             complement(157..537)
                     /locus_tag="PK612_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95506.1"
                     /translation="MALLDITKGEILSALGVDLEFELAIDDDPSGKVKRFINDVQEWC
                     YDYLRFHYGLNEDISVALAWRQDYFKKGVIKQIEYVLRNGKLSIDSGFIRSTSLVIDL
                     KGLELAPDAKQKFFLGGFCNIVRA"
     gene            complement(684..1037)
                     /locus_tag="PK612_05045"
     CDS             complement(684..1037)
                     /locus_tag="PK612_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HOH95507.1"
                     /translation="MRIAYQLPNPVNRLQSSEDARLPFAVCLQTAHKVVLAASPDGAD
                     GWVGDGLSITLSADVANVKIYYTTDGSTPTTASTLYDPAKGITITATKTIKALGVSNG
                     CTNSDVLSKTFTKSA"
BASE COUNT          332 a          235 c          214 g          323 t
ORIGIN      
        1 tgacaatcgg acgccatgca aaatcactct ttgcataaat acgccctaat ggttttgttc
       61 cggggattag tttattattt ttgacagcat taaaatcata ataataatat gtgtcggggt
      121 attcaattcg cccatagcgt aaatccacgc ccataattaa gccctcacta tattgcagaa
      181 accgccaaga aagaactttt gctttgcgtc tggtgctaat tctaaacctt tcaagtcgat
      241 gaccaatgat gtcgagcgaa taaaaccact atcaattgat agtttaccat tacgcaacac
      301 atattcaatt tgcttgataa cacctttttt gaagtagtct tgtcgccagg cgagtgccac
      361 actaatatct tcgttcaaac cataatgaaa acgcaagtaa tcatagcacc attcttggac
      421 atcattgata aagcgtttaa ctttaccact tggatcatca tcaatcgcca actcgaactc
      481 taaatcaaca cccaacgcgc ttaatatttc gcctttggtt atatccaata atgccatatt
      541 gtgtaatctc ctttatttaa ctatctattt ttattgtata ttacacgacg cataaattaa
      601 acaaaaagaa aagcgacaga tttttagtcc atcgctttaa tgtgaaatcg caaaagggta
      661 agcgattaag cacctatgct aaattaagct gatttggtaa atgtctttga taggacatcg
      721 ctattggtgc atccattaga aacaccgagt gctttaatgg tcttggtggc tgtaatcgta
      781 atgcccttag cggggtcata gagcgtggaa gcggttgttg gcgtcgagcc atcggtagtg
      841 taatagattt tgacattagc gacatcggct gacaaggtga tgctaagacc atcgcccacc
      901 caaccatccg caccatctgg cgaagcagcc aagacaactt tgtgagcggt ttgtaaacat
      961 accgcaaatg gtaatctcgc atcttcactt gattgtagac gattgacggg gttaggcaat
     1021 tgataggcaa ttctcatgac cgcacgaaga gcgaccatat cttgctgggc aaggttataa
     1081 agaattgtac catcggttgg gtct
//
LOCUS       DAOSEX010000320         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_141927_length_1100_cov_2.370335, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000320 DAOSEX010000000
VERSION     DAOSEX010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141927_length_1100_cov_2.370335"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..428
                     /locus_tag="PK612_05050"
     CDS             63..428
                     /locus_tag="PK612_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOH95508.1"
                     /translation="MGLKPSGEMYLKTIYILTKTVHPVRSIDIAEYMNFSKPSVSRAV
                     GNLRKEKYISVNEDGHIFLTPKGLDAAQNIYDRHKVLTKALRILGVSEETALNDACEI
                     EHIISDESFEALKQYLKNK"
     gene            complement(711..>1100)
                     /locus_tag="PK612_05055"
     CDS             complement(711..>1100)
                     /locus_tag="PK612_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95509.1"
                     /translation="IQGIVAKENVTATAETLASIIDVDGFTGIVEAIGLSNAAVVAFS
                     VFNLLTIPCFAAIATAKGELSNRKVYRWTIAYWFLLSYGLGALTYVSFAYVWTLAITI
                     PVIALLIALLIIYDRHKKKQELLLENE"
BASE COUNT          371 a          202 c          184 g          343 t
ORIGIN      
        1 gaaaggagat gtattaccta atcgaagtac cggggcatga ttagataata caagtcggtt
       61 atatgggtct aaaaccatct ggtgagatgt atttaaaaac aatttatatt ttaactaaga
      121 cagtccatcc tgttcggtca attgatattg ctgaatacat gaatttttct aaaccaagtg
      181 tgagtcgcgc tgtcggtaat ttacggaaag aaaagtatat aagcgttaat gaagacggtc
      241 acattttcct tacgcctaaa gggcttgatg ctgcacaaaa tatatatgac agacataaag
      301 tgctaactaa ggcgctgcgc atcttagggg taagtgaaga gacggcactt aatgacgctt
      361 gtgaaatcga acatattatt agcgatgaat cctttgaagc acttaaacag tacttaaaaa
      421 ataaataata cttaaacttc ataaaaataa agggctgctt atgaatataa tcttcattaa
      481 cagcccgttt ttaataatca tttcttgttt tatttaagcg acttataatg tttacgataa
      541 tatgacttaa tgtcaatatt tgtcgtggga cacccaccac aactaacccc acatcctgtt
      601 tcgccttttt gtcttttttt aacaaaataa agaatagtgc tagttagtac aacaataata
      661 aatataatcg tgaatatttc tgtccaatgc aaaattttaa tcttcctttg ttattcattt
      721 tctaatagta attcttgttt ctttttatgg cgatcataaa taataagcaa ggcaattaat
      781 agtgctataa ctggaatcgt tatcgccagt gtccacacat aagcgaaact aacataagtt
      841 aatgcgccta acccataact taaaaggaac caataagcaa ttgtccaccg atatactttg
      901 cgattactta attcaccttt agctgtagca atagccgcaa aacaaggaat agttagtaag
      961 ttgaacacgc taaaggccac aacagcagcg ttgcttagtc caatcgcttc gacaataccg
     1021 gtaaacccat ctacatcaat aatactcgct agtgtttccg ctgttgcggt aacattttct
     1081 ttggccacta taccttggat
//
LOCUS       DAOSEX010000321         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_142899_length_1095_cov_2.524038, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000321 DAOSEX010000000
VERSION     DAOSEX010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142899_length_1095_cov_2.524038"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..148
                     /locus_tag="PK612_05060"
     tRNA            65..148
                     /locus_tag="PK612_05060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Leu,seq:gag)
     gene            310..474
                     /locus_tag="PK612_05065"
     CDS             310..474
                     /locus_tag="PK612_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95510.1"
                     /translation="MTNTNIKLITEKTKHELFILKIDVILVRHGLKRTQIVYDRAGQV
                     AVSFVPPKRK"
     gene            537..>1095
                     /locus_tag="PK612_05070"
     CDS             537..>1095
                     /locus_tag="PK612_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95511.1"
                     /translation="MNTFKTKWKEGITKIDSFFNMLFDYFMKFFRKYYLWIAFAFILA
                     ISFFVRYAFFDYLSGDMHNFLLQWFNHLKANGGLKAMGDYPWTNTPGVRAGDYPVSYI
                     TLLALLSYLPFEGIVSIKLLSVFFDYVLAIGAVLIIREFNKNSYFSLLAFAILVFFPT
                     SILNSAVWGQCDQLYVALIVWTVLFL"
BASE COUNT          330 a          180 c          196 g          389 t
ORIGIN      
        1 aataacaatc ttttgtatta ttgtgattga aacatttata aaagttgtta taatttaatt
       61 cgacgcagtc atggcggaat tggtagacgc gtacgtttga ggggcgtatg gtaatcccgt
      121 gtgagttcaa gtctcactgg ctgcaccagt tatcaaaaat tagtcctact tcgtagggct
      181 ttttttatat ttcatcatag tcattttaaa agttatgaga tacaataaaa catatacagt
      241 ggttagtaac attaacacta atattaatgc ccctcattaa taaccgcgac acggaaaggg
      301 gttttagcta tgactaacac taacattaaa cttatcaccg aaaaaacaaa acacgaatta
      361 tttattttaa aaattgatgt catacttgta agacacggtt taaaaagaac gcaaatagtt
      421 tacgaccgag caggtcaagt agcggtgagc ttcgtgccgc ctaaaagaaa atagtattcc
      481 acgcaataag taatatttta atatatggta aactttactt aagtaagagg atgcaaatga
      541 atacttttaa aactaaatgg aaagaaggta ttacgaaaat tgactcattt tttaatatgc
      601 tttttgatta ttttatgaag ttcttccgca aatattactt atggattgct tttgccttta
      661 ttttagctat ttcctttttc gtccgttatg cattttttga ttatttaagt ggcgacatgc
      721 ataactttct cttacagtgg tttaatcact taaaggctaa tggggggtta aaggcaatgg
      781 gtgattaccc gtggactaac acacctggtg ttagagcagg ggactatcct gtatcttaca
      841 taacactgct cgcccttctt tcgtatttac catttgaagg gattgtttca attaaattac
      901 ttagtgtctt ttttgattat gtactcgcta ttggagcggt acttataatt cgtgaattta
      961 ataaaaactc ttatttctcg ctattagcgt ttgccatttt agtatttttc cccacgagca
     1021 ttcttaattc cgctgtgtgg ggtcaatgtg atcaactcta tgttgcgctt atcgtctgga
     1081 cagtattatt cctta
//
LOCUS       DAOSEX010000322         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_143100_length_1094_cov_2.816169, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000322 DAOSEX010000000
VERSION     DAOSEX010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143100_length_1094_cov_2.816169"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..>1094
                     /locus_tag="PK612_05075"
     CDS             200..>1094
                     /locus_tag="PK612_05075"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOH95512.1"
                     /translation="MAQAILSIYPGAKFGIGPDISEGFYYDVDFGDVVINDGDLAVIE
                     AKMMELSRADHRIERQVVSKEKALEIFAKNEYKVELINEIEERITIYSQGDFVDLCYG
                     PHMPSTRYVQHFKLLSLAGAYWRGDASRKQLTRIYGVAFFSKDALDKHLYNLEEAKKR
                     DHRKLGRELGLFMLSEYGPGFPFWLQKGMTLYHELEHWWIKKQREAGYEFVKTPQILS
                     RELWETSGHWLNYRENMYTTLIDERDFAIKPMNCPGSILIYNNGLHSYKDLPIRMAEH
                     GHVHRHEPSGSLNGLFRVRSFT"
BASE COUNT          344 a          194 c          227 g          329 t
ORIGIN      
        1 aaaagttaat gacggcacaa caggttttga cttacttaaa attttaaaca tcgaagggag
       61 aagtaatgtt ttagtttata aactaaacgg tgaacttttt gaccttgcaa ctccagttga
      121 gcaagatggt gaattttcct ttgtgacaaa agaggacgaa gaagcttttg cgattttaaa
      181 tcatgacgct gcccacctca tggcacaagc aattttaagt atttatcctg gcgctaaatt
      241 tgggattggt cccgatatta gtgaaggctt ttattatgat gttgattttg gtgatgttgt
      301 tataaacgat ggcgatttag cggttattga agccaaaatg atggaactct ctagagcaga
      361 ccaccgtatt gaacgccaag ttgttagtaa agaaaaagca ttagaaatct ttgctaaaaa
      421 tgaatataaa gttgaactta ttaatgaaat tgaggagcga attactattt attcgcaagg
      481 tgactttgtt gatttatgtt acggtccaca tatgccaagt acgcgttatg tccaacattt
      541 taaactgctt agtttagcag gagcctattg gcgtggtgac gcaagtcgta aacaattaac
      601 gagaatttat ggggtcgcct tcttctccaa agatgcactt gataagcatc tctataacct
      661 tgaagaagca aaaaaacgtg accaccgtaa attaggtcgc gaacttggac tatttatgct
      721 tagtgaatat ggtccaggct tccccttctg gctccaaaaa ggaatgactc tttatcacga
      781 acttgaacac tggtggatta aaaaacaacg cgaagcaggt tacgaatttg tcaaaactcc
      841 gcagatctta agtcgtgaac tatgggaaac aagcggacac tggcttaatt accgtgaaaa
      901 tatgtacaca actcttattg atgaacgcga ttttgccatt aaaccgatga attgtcctgg
      961 ttcaatcctt atttataata atggtttaca ttcatataaa gatttgccaa tccggatggc
     1021 tgaacatggt catgtccacc gtcatgaacc aagcggttca cttaacggat tattcagagt
     1081 gcggagtttc actc
//
LOCUS       DAOSEX010000323         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_144012_length_1090_cov_1.749758, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000323 DAOSEX010000000
VERSION     DAOSEX010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144012_length_1090_cov_1.749758"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PK612_05080"
     CDS             complement(<1..1080)
                     /locus_tag="PK612_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH95513.1"
                     /translation="MGVYNWANTFVYYFLIISRLGIPLIAIRECAKVKDDKDKLSEKV
                     QSFFVLQLITTALSFVLLSTIMFIGKDTPMFKGDTWELIFLLSLNFLVGVFSFEWVYI
                     ALDRVFYMSVRSLATTILATLLIIVFVQNDHDLFIYAFINLIATFVTVTLNLLLLKRE
                     GIKLSFRRKVNLKHLTKSLLFVFGITFLITAYNQNDSLLLGYLDDSFRTSGAYSVGIR
                     GIEIVITIITSLSGIFVIRTTSALKNDDMPAFHRVISYSTNITFFIGVPAVLFMIGLS
                     PEIVGFIVKDSPYWDEAAINNAILAVVLLSSLMLTYSIHDGIYQQVLVPLKKEKIYFF
                     TMLFVLILNIVASVLLALFVFKDNPL"
BASE COUNT          434 a          184 c          182 g          290 t
ORIGIN      
        1 aagcggatta tctttaaaca cgaataaggc aagtaataca cttgcaacga tatttaaaat
       61 aagcacaaat aacatagtaa agaagtatat tttttctttt ttaagcggaa caagcacttg
      121 ctgatatata ccgtcatgaa tactgtaagt tagcattaaa gatgaaagca gaacaaccgc
      181 taaaatggcg ttattaatcg ctgcttcatc ccaataagga ctatctttaa caataaagcc
      241 gacaatttcc ggtgataagc caatcataaa taagaccgca ggtacaccaa taaaaaaggt
      301 aatattagtg ctgtaactta taacacgatg gaaagcaggc atatcatcat ttttaagtgc
      361 agaagtagtg cggatcacaa agataccact taaagaagta ataatagtaa taacaatttc
      421 aataccgcga attccaacag aataagcacc agatgttcta aaagaatcat ctaaatagcc
      481 aaggagtaaa gaatcatttt ggttataagc ggtaattaag aaagtaatac caaatacaaa
      541 gagaagcgac tttgttaagt gttttaaatt aacctttcgt ctaaaggaaa gctttatacc
      601 ttcgcgtttt aataagagaa ggtttaaggt cacagttaca aaagtagcga ttaagttaat
      661 aaaagcataa ataaataaat cgtgatcatt ttgtacgaaa acaataatca gtagtgtagc
      721 taaaattgtt gtcgctaaag aacgaacgga catataaaac actcggtcta gagcaatata
      781 tacccattca aaggagaaga cgcccacaag gaagtttagt gataataaaa agattaactc
      841 ccaagtatca cctttaaaca tcggtgtatc tttaccaata aacataattg tggacaaaag
      901 cacaaaggaa agtgccgttg tgattaattg cagtacaaag aaagactgaa ctttttcact
      961 tagtttatct ttatcatctt tcaccttagc gcattcccta attgcaataa gcgggatacc
     1021 aagacgcgaa ataattaaaa agtaatagac aaaagtattt gcccagttat atacccccat
     1081 cgccgcagta
//
LOCUS       DAOSEX010000324         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_144082_length_1089_cov_3.346228, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000324 DAOSEX010000000
VERSION     DAOSEX010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144082_length_1089_cov_3.346228"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..>1089
                     /locus_tag="PK612_05085"
     CDS             107..>1089
                     /locus_tag="PK612_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOH95514.1"
                     /translation="MAKKEIIIGIDLGTTNSVVSYRQADGQIKVIPTPEGKPTTPSVV
                     AFKPDGEQVNGEAAKRQAVTNPDTVMSVKRHIGTNKKFKINALKKEFTPEEISARILG
                     YMKDYAEKNLGQSIQKAVITVPAYFNDAQRQATKVAGEIAGLEVVRIINEPTAAALAY
                     GLDKEHDEKILVFDLGGGTFDVSILEIGDGTFEVIATAGDNHLGGDDWDAEIVEWIKQ
                     EIEREHGLKLNDKMAMQRFREAAEKAKIDLSQMLETTISLPFIGMGASGPISYEGKLT
                     RAKFLDLTKHLLARVEEPIRRALSDAKVKASDLNQVILIGGSTRMPAVQDLV"
BASE COUNT          386 a          184 c          250 g          269 t
ORIGIN      
        1 agttatggtg tcaacgaata ttaaagaaga accaaaaaat gatgataatg tcccagaagt
       61 taatcctgag gacttaaatt aataaaaagg agaagagata tatattatgg caaagaaaga
      121 aattattatt ggaattgact taggaacaac taatagtgtc gtaagctatc gccaagctga
      181 cggccaaatt aaagttattc caaccccaga aggcaaaccg accaccccaa gtgtcgtcgc
      241 ctttaaacct gatggcgaac aagttaatgg tgaagcggcc aaacgtcaag cggttactaa
      301 tcctgatact gtgatgagtg ttaagcgtca catcgggaca aataaaaagt ttaaaattaa
      361 tgcgttaaag aaagaattta caccagaaga aataagcgcg agaattcttg gttatatgaa
      421 agattatgcc gaaaagaatt tagggcaaag tattcaaaaa gctgttatta ccgttcctgc
      481 ttactttaat gatgcgcaac ggcaagcaac aaaagtcgct ggtgaaattg ccggccttga
      541 agttgtgcgg attattaacg aaccgaccgc agcagcactt gcgtacggtt tagataaaga
      601 acacgatgaa aagatcttag tgtttgactt aggtggtgga acatttgacg tttcaatctt
      661 agagattggt gatggtacct ttgaagttat cgcgaccgct ggagataacc acttaggtgg
      721 ggacgactgg gacgcggaga ttgtcgaatg gattaagcaa gaaattgaaa gagaacatgg
      781 cttaaagtta aatgacaaaa tggctatgca acgcttccgt gaagcggctg aaaaagcaaa
      841 gattgactta agtcaaatgc ttgaaacaac aattagttta ccctttattg gtatgggcgc
      901 aagcggacca attagttacg aaggtaaatt aacaagagcg aagttccttg atttaacaaa
      961 acacttatta gcgagagtcg aagaaccaat tcgccgggca ctaagtgacg ctaaagttaa
     1021 agcaagtgac cttaaccaag taatcttaat tggcggtagt acaagaatgc ctgctgtcca
     1081 agatttagt
//
LOCUS       DAOSEX010000325         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_144517_length_1087_cov_3.687984, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000325 DAOSEX010000000
VERSION     DAOSEX010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144517_length_1087_cov_3.687984"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /gene="ssb"
                     /locus_tag="PK612_05090"
     CDS             complement(<1..190)
                     /gene="ssb"
                     /locus_tag="PK612_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003774356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH95515.1"
                     /translation="MINRVVLVGRLTHDPELRKTSSGASVTNFSIAVNNRFARDQENS
                     TSFFRVSCWNQTADFVAQY"
     gene            complement(209..496)
                     /gene="rpsF"
                     /locus_tag="PK612_05095"
     CDS             complement(209..496)
                     /gene="rpsF"
                     /locus_tag="PK612_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOH95516.1"
                     /translation="MKKYEIMYILLADLSEEERKAEMESLHQMITNLGGKIVDVDTEN
                     WGLRDFAYPINDLLKGYYVVINVESDAAAIAEFERLSKLNKNVIRHLVIAK"
     gene            complement(645..>1087)
                     /locus_tag="PK612_05100"
     CDS             complement(645..>1087)
                     /locus_tag="PK612_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018167086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOH95517.1"
                     /translation="IHVEKHWTGSGHILNAFFEEFCEADLIQPTFVYGHPLEISPLAK
                     KAEDPRFTERFELFIKANELANAFTELNDPRDQYERFKAQLKARELGDEEASEMDEDF
                     VEALEYGMPPAGGIGIGIDRLVMFLLNQTSIREVILFPTMRDNK"
BASE COUNT          303 a          218 c          194 g          372 t
ORIGIN      
        1 cgtattgagc gacaaagtct gcggtttggt tccagcatga gactctaaag aaacttgtac
       61 tattttcttg gtcgcgagcg aaacgattgt ttactgcgat gctgaagttg gtaacggatg
      121 cacctgacga tgttttacgt aattctgggt cgtgggtaag tcttcccact aagactactc
      181 gattaatcat tgttattcct cctcgttttt atttagcgat aacaagatga cgaattacat
      241 ttttatttaa ttttgataac cgttcgaatt cagcgattgc cgcagcatca ctttcgacat
      301 taatgactac ataatatcct ttaagtaaat cattaatcgg atacgcaaaa tcacgtaagc
      361 cccaattttc ggtatctaca tcaacaattt taccgcctaa attggtaatc atttgatgga
      421 gactttccat ttcggcttta cgttcttctt cgcttaagtc tgcaagaaga atatacatga
      481 tttcgtactt tttcataagc acacctccct ttggtctatt tcggctgttg cttaccaaca
      541 gcatggagta tattacaagc agtaatatag cgttgtaatt atattactat tactagcgtt
      601 tgtaaaggca caccttgttc gctagtaata attacaattt tattttattt attatcacgc
      661 attgttggga aaagaataac ttcccgaatt gatgtttgat ttaataaaaa cattacaaga
      721 cgatcaatac cgattccaat cccgcctgcc ggtggcatcc cgtattctaa tgcttcaacg
      781 aagtcttcgt ccatttcact agcttcttca tcacctagtt ctcgcgcttt taattgtgct
      841 ttaaagcgtt catattgatc gcgcggatca ttaagttcag taaaggcgtt cgctagttcg
      901 tttgctttaa taaataattc aaagcgttct gtaaaacgcg gatcttcggc ttttttcgct
      961 agtggggaaa tttcaagtgg atgcccatat acaaatgttg gttgaattaa gtctgcttca
     1021 cagaattctt caaagaaagc attaagaata tgacccgagc cagtccagtg cttttcaaca
     1081 tgaattt
//
LOCUS       DAOSEX010000326         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_145606_length_1082_cov_2.653359, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000326 DAOSEX010000000
VERSION     DAOSEX010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145606_length_1082_cov_2.653359"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PK612_05105"
     CDS             <1..1079
                     /locus_tag="PK612_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95518.1"
                     /translation="EMFLNDYYRGLELVAKSANLDFTEALLADVGRLFNVPPLSNYIY
                     RTTEIKTGRQVSVINHVHPHVPVNQISDAIADFYAFMATSTLPTIVQSAAIIFYLDYI
                     KPFEAHSEIIAILMAKAFLAERELALIAPYLNFELILERYRDARDDVFAEVKKSADLT
                     YFIDLLINVLNESLQTIEERIVFVVTHELEAEKHVLPPFEEETQEESVSATPPTDLFE
                     HAQAEVAEKEETFKSEPVPVYTPKKSAIPLPLPPREEGVKVTPEEANLGITQLPPELS
                     ENDILRLERHIRESDPSLSRAQAYFYARHCTMGKYYSINDFKEVTGCAYETARTSMEH
                     LANSGYYRKEKFKNKFIYTPKRKA"
BASE COUNT          356 a          213 c          209 g          304 t
ORIGIN      
        1 gggagatgtt tttaaatgat tattatcgcg ggttagaact agtagcaaaa agcgctaatc
       61 ttgattttac tgaagcactt ttagctgatg ttgggcgcct atttaatgtt ccgccgctaa
      121 gtaactatat ttatcgcact accgaaatta aaacggggcg tcaagtctcc gtcattaatc
      181 atgttcatcc gcatgttccc gtcaatcaaa ttagtgatgc aatcgctgat ttttacgcat
      241 ttatggccac aagtacatta ccaaccattg tgcaaagtgc agcgattatt ttctatcttg
      301 attatattaa accttttgaa gctcatagtg aaattattgc cattttaatg gcaaaagcct
      361 tccttgcaga aagagaactc gcgcttattg cgccttatct taactttgaa cttatcttag
      421 aaagataccg tgatgcgcgt gatgatgttt tcgccgaagt aaagaaaagt gctgatttaa
      481 cttattttat tgatttactt attaatgtgc ttaatgaatc actgcaaaca attgaagaac
      541 ggattgtgtt tgtcgtgacg catgaacttg aagcagaaaa gcatgtgctc ccaccatttg
      601 aagaagaaac gcaagaagaa agtgtgagtg ctacaccacc aactgatctt tttgaacatg
      661 cccaagccga agtagcggag aaggaagaaa cttttaaatc tgaaccagta ccagtttata
      721 caccaaaaaa atccgcaatc ccgcttccac ttccgccgcg cgaagaaggc gtaaaagtta
      781 caccagaaga ggccaattta gggattacac aattaccacc agaacttagc gaaaatgata
      841 tattacgctt agagcgacat attcgtgaaa gcgatccaag tttaagtcgc gctcaagctt
      901 atttctacgc tagacactgt actatgggta aatattactc aattaatgac tttaaagaag
      961 tgacaggctg tgcgtatgaa acagcgcgga catcaatgga acacctcgct aacagcggat
     1021 attaccgcaa agaaaaattt aaaaacaaat ttatttatac accaaaacgg aaagcatagg
     1081 at
//
LOCUS       DAOSEX010000327         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_146825_length_1076_cov_4.804114, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000327 DAOSEX010000000
VERSION     DAOSEX010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146825_length_1076_cov_4.804114"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PK612_05110"
     CDS             complement(<1..307)
                     /locus_tag="PK612_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH95519.1"
                     /translation="MKKSKDEKRNKIIEHKKRGLNYYNVAARGFEESKKLKIAPKDAI
                     ISLQNVNKIYPNFVQAVFDFSLDIKPQEFIVFVGPSGCGKSTTLRMIAGLEEITAGDL
                     "
     gene            complement(304..>1076)
                     /locus_tag="PK612_05115"
     CDS             complement(304..>1076)
                     /locus_tag="PK612_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95520.1"
                     /translation="CVDIVRSFYGGKFPIGMYYGKKVECNEDQHIFDIDLVDHYKDLI
                     KETNYIEAYKLYRKVLAEAEDDSIIFITIGEFTNAANLVSSLPDEISPLSGQELFAKK
                     VKYSCVMGGLINDNHPITAFNNKAFPEYNIVCAIEDAQKFVKISAKPIYFLDFYVGAD
                     VFTGKKVIEHDEHSPMKFAYLKYANGPSRSWDLLAVYYAITKRLYRSVGPGVMHIDDN
                     GFTTFTKDQDGHHYYLRPIKSNEEITKEIDEALVGNIL"
BASE COUNT          343 a          201 c          145 g          387 t
ORIGIN      
        1 ataaatcacc cgcagttatt tcctctaagc ccgcgatcat tcttagtgtt gtggacttac
       61 cacagccaga aggaccgaca aagacaataa attcttgcgg ctttatatct aacgaaaaat
      121 caaaaacagc ttgaacaaaa ttaggatata ttttatttac attttgtaag gaaatgatgg
      181 catctttcgg tgcaattttt agtttctttg attcttcaaa accgcgcgcc gcaacgttat
      241 aataatttag ccctcgtttt ttatgttcaa ttattttgtt tcttttttca tcttttgact
      301 ttttcataat atgttcccca ctagtgcttc atctatttct ttagtaattt cttcattact
      361 tttaattggt cttaaataat aatggtggcc atcttgatct ttagtaaatg tcgtgaatcc
      421 gttatcatca atatgcatta ccccaggccc aaccgatcta tataagcgtt tagtaattgc
      481 ataataaacc gctaataaat cccaacttcg cgatggcccg ttagcgtatt ttaaatatgc
      541 aaatttcatt ggtgaatgtt catcatgttc aattactttc tttcctgtga aaacatcggc
      601 gccaacataa aaatctaaaa aatagatggg ttttgccgag attttaacaa acttttgtgc
      661 atcttcaatc gcacaaacaa tattatattc agggaacgct ttattattaa aagcagtaat
      721 aggatgatta tcatttatta atccacccat tacacaacta tactttactt ttttagcaaa
      781 taattcttgg ccagataatg gtgatatttc atcaggtaaa gaacttacca aattcgctgc
      841 attagtgaat tcaccaatcg tgataaaaat aattgaatca tcttctgctt cagcaagaac
      901 tttacgataa agtttatatg cttcaatata attagtttct tttattaaat ctttataatg
      961 atcgactaaa tcaatatcaa aaatatgttg gtcttcgttg cattcaactt ttttgccata
     1021 atacattcca attggaaatt ttccgccata gaaagaacgc acaatatcaa cacatt
//
LOCUS       DAOSEX010000328         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_146911_length_1076_cov_2.368266, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000328 DAOSEX010000000
VERSION     DAOSEX010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146911_length_1076_cov_2.368266"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PK612_05120"
     CDS             complement(<1..310)
                     /locus_tag="PK612_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOH95521.1"
                     /translation="MKKFTPNYYAKNIYEVSADFYLKHGIKTLLVDLDNTLASYRELE
                     PSDTTINFVRNLEKAGIKVLLASNNSGKRVKRYGELLGVPYISGARKPFAFKLRKFLK
                     E"
     gene            456..725
                     /locus_tag="PK612_05125"
     CDS             456..725
                     /locus_tag="PK612_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95522.1"
                     /translation="MNLSKLKPYRNMLFLALAAGVGAFMPVIGIIVTVVMYAKRDENS
                     LNFTKEERFLLNALLIILIIYLTLNVLYTLKYPEVKPDTSSETSL"
     gene            complement(722..>1076)
                     /locus_tag="PK612_05130"
     CDS             complement(722..>1076)
                     /locus_tag="PK612_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95523.1"
                     /translation="LQHVMRTFKSDPHTIVSAFRYNRNIEELRVYALTDANVILPLLL
                     TDLAPTPVDKYAKRNINELAEEEKIVSIFNEEIPTISEETKAYAPIVKAEAVKVEPVE
                     DSYEQISIFDLLDED"
BASE COUNT          325 a          185 c          175 g          391 t
ORIGIN      
        1 gctctttaag gaatttacgg agcttaaacg caaatggttt acgagctcct gaaatatacg
       61 ggacaccaag taattcacca tatcgtttaa cgcgtttacc actattattt gatgctaaaa
      121 gtactttaat accagctttt tctaagttgc ggacaaaatt aatagttgtg tcacttggtt
      181 ctaattcacg ataactcgct agtgtgttat ctaaatcaac gagtaaggtt ttaatcccgt
      241 gcttaaggta gaagtcagca cttacttcat atatattttt agcgtaataa ttcggtgtaa
      301 atttcttcat aatatctgtc acttctaaag tataaggtta ttttatcatg gacgatataa
      361 ttctcgctta acaacagtaa atctttttat aaaagattaa acctttaaat ccttcttttc
      421 ttataaatcg ttagcgtatg tgctacaatt tagttatgaa tttaagtaaa ttaaaaccgt
      481 atcgtaatat gctcttctta gcgcttgccg ctggtgtcgg tgcttttatg cccgtcattg
      541 ggatcattgt tacagttgta atgtatgcaa agcgtgatga aaactcgctt aattttacaa
      601 aagaagagcg ttttttacta aatgcccttt taattatatt aataatatat ctcacactta
      661 atgtgctcta tactttaaaa tatcctgaag ttaaaccaga tacaagtagt gaaacttcac
      721 tttaatcttc atctaataaa tcaaagatac taatttgttc ataagagtct tcaaccggct
      781 ctacttttac ggcctctgct tttacgattg gggcataagc ttttgtttct tcgctgatag
      841 tggggatttc ttcattaaag atactaacaa tcttttcttc ttcggcgagt tcattaatat
      901 tgcgttttgc atatttatca actggcgttg gcgctaggtc agttaagagc aatggtaaaa
      961 taacattcgc atcagttaaa gcatacactc ttaattcttc aatatttcga ttataacgga
     1021 aggcactgac aattgtgtgt ggatcacttt taaaagtgcg cattacatgt tgtaat
//
LOCUS       DAOSEX010000329         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_147181_length_1075_cov_1.905882, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000329 DAOSEX010000000
VERSION     DAOSEX010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147181_length_1075_cov_1.905882"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..>1075)
                     /locus_tag="PK612_05135"
     CDS             complement(222..>1075)
                     /locus_tag="PK612_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95524.1"
                     /translation="LLAFTLLLQVWFDITQFNWFEFTFSIINFVAGRGIYFPMGLDVG
                     MAQPDYMLIANTLKKYRNLIIKKRVNKEFRERLKVHNQLSKCEAYLDILDNQLLSKKI
                     TDSEILKIEAEKSDVLAYIDFLNGKAPEYKGTLNLATLRVKYDYTTFDNMFAFGTGNR
                     KVGSKYISHPYMTGLRFSAMPFIMTIIIAFANAILVFAEYGVNTASILVFVIKLTMFA
                     WGCYQGIQTGKRVVDEDKYPAILNLMSLGKEIITEIEIANNIVIDEAPLPKPLNEDAP
                     KPEITEL"
BASE COUNT          311 a          216 c          174 g          374 t
ORIGIN      
        1 ctttaagtca tcgcgactaa tgctgatact tttggtttgc gttccatctc ttttgacttc
       61 cgttagtgta atcatctttt tctccttgcg atttcttttc gcacctttat cttaccacat
      121 aaaaaacgaa tagcaaacac ttttttaact tttttttatt cggttgctat ttatataata
      181 aaaaagacct aacaagtagg tcaatttatt cgatgttgtg cttataattc ggtgatttct
      241 ggtttcggcg catcttcgtt taatggtttc ggtaatgggg cttcgtcgat cacgatatta
      301 ttagcgattt cgatttcggt aattatctct tttccaagcg acataaggtt taatattgcc
      361 gggtatttat cttcatcgac cacgcgttta cctgtttgta taccttgata acatccccac
      421 gcaaacattg ttaacttaat cacaaacact aagattgaag cagtattcac gccatattcg
      481 gcaaacacca gtatcgcgtt agcgaaagcg ataattatgg tcattatgaa tggcattgca
      541 gaaaatctca aacccgtcat atatggatgc gatatatatt tactcccaac tttccgattg
      601 cccgttccaa atgcgaacat attatcaaag gtagtgtagt catatttaac gcgtaatgtg
      661 gctaaattaa gcgttccctt gtattctggc gctttaccat taaggaagtc aatatatgcc
      721 aacacatcgc ttttttcggc ttcaatttta aggatctcgc tatcagtaat cttctttgat
      781 aacaattggt tatctaatat atctaaatat gcctcgcatt tcgataattg gttatgcact
      841 ttcaatcgtt ctctaaactc tttattgacg cgctttttaa taattaagtt gcgatatttt
      901 ttgagtgtat tagcaattaa catataatct ggttgtgcca ttccaacatc taaacccatc
      961 gggaaataaa taccgcgacc agcgacaaag ttaatgatgc taaatgtaaa ctcaaaccaa
     1021 ttaaactgcg tgatgtcaaa ccatacttgc aatagcaaag taaaggccag caacg
//
LOCUS       DAOSEX010000330         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_148638_length_1068_cov_2.396841, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000330 DAOSEX010000000
VERSION     DAOSEX010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148638_length_1068_cov_2.396841"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PK612_05140"
     CDS             complement(<1..660)
                     /locus_tag="PK612_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH95525.1"
                     /translation="MFKNLRKFIEEHDKIAIFSHIYPDGDAVGSIIGLRELIKTSYPH
                     KEVFGLGSNVQPFVSIVGELDEVSDDFIKESAAIIVDVANGERVEDQRFKLAKASFKI
                     DHHIFVEKFTDEELVLTKRIATAEIIGELMMKEKLRTNKLGATALALGIITDSGRFRY
                     DLTSGNTFSVMARLTNIGADLALINENLAKRSLADFKKRGHFLSNYETYENVIYIIVP
                     YL"
     gene            complement(661..>1068)
                     /locus_tag="PK612_05145"
     CDS             complement(661..>1068)
                     /locus_tag="PK612_05145"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003253712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOH95526.1"
                     /translation="ETLYADPNECLEMVQKTKIDCLAPALGSVHGPYKGEPKLGFDEM
                     DHINKLVGVPLVLHGGSGIPDFQIQMAIERGTAKINVNTEIQQEWTKIVRETLANNDK
                     VYDPRKIIGPGMKGIRKVVNEKCRVFGCIGKAA"
BASE COUNT          325 a          235 c          161 g          347 t
ORIGIN      
        1 taagtatggg acaataatat aaattacatt ttcataagtt tcataatttg ataaaaagtg
       61 tccgcgtttt ttaaagtcag cgagtgaacg cttcgctaaa ttctcattaa ttaacgctaa
      121 atccgcacca atattagtga gtcgcgccat cacactaaaa gtgttaccgc ttgttaagtc
      181 gtatctaaaa cgacctgaat cggtaataat cccaagcgct aaggcagtcg caccaagttt
      241 atttgtgcga agcttttctt tcatcattaa ttcaccaata atttcggcag tagcaatacg
      301 ctttgttaac actaactctt catcagtaaa cttttccacg aaaatatgat gatcaatttt
      361 aaatgatgct tttgctaatt taaagcgttg atcctcaact ctctcaccgt tagcgacatc
      421 gacaataatc gctgcgcttt ctttaatgaa gtcgtcactt acttcatcta actcgcccac
      481 aatagaaaca aagggttgga cattactgcc caacccaaaa acttctttat gcggataact
      541 tgtttttatt aattcacgca agccaataat actcccaact gcatcgccat caggataaat
      601 gtgtgaaaag attgcaatct tatcatgctc ttcaatgaac ttacgtaaat ttttaaacac
      661 ttatgcagct ttccctatac aaccaaagac gcggcacttt tcgttaacaa ctttacgaat
      721 gcctttcatt cccggaccaa taattttacg tggatcataa actttatcat tattcgctaa
      781 agtttcacgg acaatctttg tccattcttg ttgaatttca gtgttgacat taattttagc
      841 ggtaccacgt tcaatcgcca tttgaatttg gaaatcagga ataccactgc caccatgtag
      901 cacaagtggg actccgacta atttattaat atgatccatt tcatcaaaac caagttttgg
      961 ttcgccttta tacggaccat gtacactacc aagcgctggc gctaagcagt caatttttgt
     1021 tttttgcacc atttctaaac attcgtttgg atcagcatat aatgtttc
//
LOCUS       DAOSEX010000331         1061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_150252_length_1061_cov_2.042744, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000331 DAOSEX010000000
VERSION     DAOSEX010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150252_length_1061_cov_2.042744"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PK612_05150"
     CDS             complement(<1..201)
                     /locus_tag="PK612_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95527.1"
                     /translation="MAFGQFSAHLIAPRVIRVVLYSDKYQYEQTRVTIYDDNDKLVPF
                     SSKSKVVQYKVTAFELTLEQPFV"
     gene            complement(207..527)
                     /gene="acpS"
                     /locus_tag="PK612_05155"
     CDS             complement(207..527)
                     /gene="acpS"
                     /locus_tag="PK612_05155"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOH95528.1"
                     /translation="MSIGIDIVYIPRFAGKEKLATRILSKNELEIYHMRPNKEEFLAG
                     RFAAKEAFLKAMKEGIGRVAFHKIDVVYKKDGRPEIHHDGNVYAVSISHDGDYAVAVV
                     QIKE"
     gene            complement(530..>1061)
                     /locus_tag="PK612_05160"
     CDS             complement(530..>1061)
                     /locus_tag="PK612_05160"
                     /inference="COORDINATES: protein motif:HMM:NF020952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOH95529.1"
                     /translation="RPSVQVITGNIKANIPSRVAFMTASVTDSMTILGCGGAENLLGY
                     GDMLVDSITTNYRGLLRVQAPYVQVREVLRVTNYLRNNYMTDFYPEFLDLKEKVNVYG
                     GGGPVISDDLYQAVLDFTLTQDTISISRLQQNFNLGWPRARQLYDQLMQNGIIEPPDE
                     ANSAKGAVVIANKRGN"
BASE COUNT          325 a          237 c          172 g          327 t
ORIGIN      
        1 cacaaaaggt tgttctagtg ttaattcaaa agcagtaact ttatattgta cgactttact
       61 cttcgaacta aacggcacta atttatcgtt atcatcataa attgtcacac gtgtttgttc
      121 atattgatat ttatcactat agagcaccac gcggattacc cgtggcgcaa ttaaatgtgc
      181 gctaaattgt ccaaatgcca taaatactac tccttaatct gtactactgc gaccgcataa
      241 tcaccgtcgt gactaattga cacagcataa acattaccgt catgatgaat ttcagggcgt
      301 ccgtcttttt tatacacaac atcaatttta tgaaaagcaa cgcggcctat cccttctttc
      361 attgctttta aaaacgcctc tttagcggca aaacgccctg caagaaattc ttctttattt
      421 ggtcgcatat gataaatttc taattcattt ttcgataaga tgcgtgtcgc aagtttttct
      481 ttacccgcaa agcgcggaat ataaacaata tcaatcccaa tcgacataat taattacctc
      541 tcttattggc aataacaacc gcccctttag cgctattagc ttcatcaggg ggttcaataa
      601 tgccattttg cattaattga tcataaagtt gacgggcacg cggccatcct aaattaaagt
      661 tttgttgtaa tctgctgata gaaatcgtat cttgcgttaa agtaaagtca agcactgctt
      721 gatagaggtc atcactaata acgggaccgc caccaccata gacattaact ttttctttta
      781 aatctaaaaa ttccggataa aaatcggtca tatagttatt gcgtaaataa ttagtgacgc
      841 ggagtacttc tcgcacctgt acataaggcg cttgtacacg taatagtccg cgataattag
      901 tcgtaatact atcaactaac atatccccgt agccaagtaa gttttccgct ccaccgcaac
      961 caagaatcgt catactatca gtcacgcttg ccgtcataaa ggcaacacgg ctaggaatat
     1021 tagccttaat attaccagta ataacttgga cacttggtct t
//
LOCUS       DAOSEX010000332         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_150513_length_1060_cov_1.776119, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000332 DAOSEX010000000
VERSION     DAOSEX010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150513_length_1060_cov_1.776119"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PK612_05165"
     CDS             <1..450
                     /locus_tag="PK612_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004803709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOH95530.1"
                     /translation="NIPAGIGEGQKLRIQGKGERGLNGGPNGDLHVEIVIKPHNSFKR
                     VGNDIYLQIPISFVDAALGVTLEVPTVYGNVEVKIPAGTQPGATLRLSGQGVKDVRGN
                     VGNQYLEVEVKTPTNLSKDQKVALEDFKKAEPKKETFFEKFKSKFKR"
     gene            714..>1060
                     /locus_tag="PK612_05170"
     CDS             714..>1060
                     /locus_tag="PK612_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95531.1"
                     /translation="MKNKKLLSKATLAFFALSALLISAISLGSNHGSSARGEPTPYAI
                     VFNASKNKIVNGPVGQSNSGYGSMKTELGNSIGMQIGALENTATTWQSLRSSGYITNT
                     TIIHGITYIRFKE"
BASE COUNT          364 a          171 c          209 g          316 t
ORIGIN      
        1 aacatcccag ccggcattgg tgaaggtcaa aagctacgga ttcaaggcaa gggtgaacgc
       61 ggacttaacg gtggtcctaa tggtgaccta catgttgaaa ttgtcattaa accgcataat
      121 agttttaagc gcgttggtaa cgatatttat ttacaaatcc ccattagctt tgtcgatgca
      181 gcgcttgggg ttacacttga agtcccaacg gtttatggca atgtcgaagt taaaatacca
      241 gcgggtacgc aacctggcgc tactttacgc ttaagtggcc aaggggttaa agacgtacgc
      301 ggtaatgttg gaaaccaata cttagaagta gaagtgaaaa caccgactaa tttaagtaaa
      361 gatcaaaaag tcgcacttga agacttcaag aaagcggagc caaagaaaga aacattcttt
      421 gagaaattta aatctaaatt taaacgctaa aatttattaa aattatatga aagaggttag
      481 gaataaatcc ctagtctttt tttgtataaa aaacgttatc acaaaaaatt gaatttttgc
      541 tttagttaat aatcaactaa attattacga atatatgcaa ttaatttaat ttaagtgaag
      601 ttttaatcct tctttttact aatttaggtc atgaaattaa aacttttaat tgattttatg
      661 gtgttaaact aaatttatga acatatgttc atttggtcgg aaggaaataa tctatgaaaa
      721 acaaaaagtt gcttagtaag gcgaccttag ccttttttgc gttatcggca ctacttatta
      781 gcgcgattag tcttgggagt aatcacggct ctagtgcgcg cggtgaacca acaccgtacg
      841 ccattgtgtt taatgcgagc aaaaataaaa ttgtaaatgg tccagttggt caaagtaatt
      901 ccggctatgg atcaatgaag acggaacttg gtaactcaat cggaatgcaa attggtgctt
      961 tagaaaacac cgctacaaca tggcaatcat taagatcaag tgggtatatt acgaatacta
     1021 ctatcattca cggcattact tatatacgtt ttaaagagta
//
LOCUS       DAOSEX010000333         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_151317_length_1056_cov_2.371628, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000333 DAOSEX010000000
VERSION     DAOSEX010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151317_length_1056_cov_2.371628"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PK612_05175"
     CDS             <1..522
                     /locus_tag="PK612_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH95532.1"
                     /translation="NETLLQWGWISRRIKFLDYPVLAKITVIVVNIWVGVPYTMLLTS
                     GILMNIPTELYESAKIDGAGPVRQFFQITMPYILFVTGPYLITQFIGNINNFNVIFFL
                     TGGGPSTGIPGVPYGHTDILITWLYALTVGGAHEEYAIGSALGIFIFLISATITLVLY
                     SRTSAAQSEGDFA"
     gene            524..>1056
                     /locus_tag="PK612_05180"
     CDS             524..>1056
                     /locus_tag="PK612_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95533.1"
                     /translation="MSKNVKKDVVTEQPTGLHASAASSRIPKGKVKFWQNQRVIRGVV
                     DAFLYVLLAAMCVAWIFPIIWLIANALRIESTGPVSYLFPREIGFDNFRRLFTTAEMP
                     YLKWFTNTLIVATFSSIISTTIVLLTSYTFSRLRFKARKGLMKFLMILGMFPGFMSMI
                     AIFHILKAIGLLPTETRP"
BASE COUNT          290 a          216 c          215 g          335 t
ORIGIN      
        1 aatgaaacac tattacagtg gggatggatt agtcgacgga ttaaattcct cgattatcca
       61 gtcctagcta aaattacggt cattgtcgta aatatttggg ttggggttcc ttacacaatg
      121 ctattaacgt cgggtatctt aatgaatatt ccgactgaat tatatgaatc agcgaaaatt
      181 gacggcgctg gacctgtccg gcaattcttc caaattacga tgccttacat cttgtttgtt
      241 acaggtccgt acttaattac ccaattcatt gggaacatta ataactttaa cgttatattc
      301 ttcctgacag gtgggggacc aagtactggt attcctggcg taccgtatgg tcacactgat
      361 attttaatta cgtggctata cgcgcttacc gttggaggag cacatgaaga gtacgcgatt
      421 ggtagtgcac tagggatctt catcttcctt atcagtgcga ctattacttt agtgctctat
      481 tcgcggacat ccgcagcaca atcggaagga gactttgcat aatatgagta aaaatgttaa
      541 aaaagatgtt gttactgaac aaccaacagg cttacacgct agtgcggcct cctcacgaat
      601 tccaaagggt aaagttaagt tttggcaaaa tcaacgtgta atacgtggtg ttgtcgatgc
      661 tttcctttat gtcttactag cggcaatgtg tgtggcgtgg attttcccga ttatttggtt
      721 aatcgctaac gcactccgca ttgagtcaac tggacctgtt tcttatcttt tcccgcgaga
      781 aatcggcttt gataacttta gacgactttt cactacagca gaaatgccgt acttaaaatg
      841 gtttacaaat acgctaattg tggcgacatt tagtagtatc atttccacaa ctatcgtctt
      901 acttacaagt tataccttta gccgacttcg ttttaaagcg cgcaaaggtc ttatgaaatt
      961 tttaatgatt ctcggaatgt tccctggctt catgtcaatg attgcaattt tccacatctt
     1021 aaaagcgatt ggattattac caaccgaaac acgacc
//
LOCUS       DAOSEX010000334         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_154139_length_1044_cov_1.843276, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000334 DAOSEX010000000
VERSION     DAOSEX010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154139_length_1044_cov_1.843276"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..275)
                     /locus_tag="PK612_05185"
     CDS             complement(72..275)
                     /locus_tag="PK612_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95534.1"
                     /translation="MNNDIDNSSFGGGFALGFLLGLIGLIIAFAIGGGNIRRGAGYGL
                     LTYFIIYTITGIIVLLVSMSGGY"
     gene            complement(272..520)
                     /locus_tag="PK612_05190"
     CDS             complement(272..520)
                     /locus_tag="PK612_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95535.1"
                     /translation="MKTLEKIFDCVYLTVISFLVIYTFFFIVIALTFALTYLIGGIEL
                     DIEVCMLNWVVISTVAFLITSSALITSYIKSEKKEVEK"
BASE COUNT          341 a          211 c          158 g          334 t
ORIGIN      
        1 ctgcttttgt cggttttgtc ggtttttcat acacatttac gaagacaccg aacgctataa
       61 ttttaataat actaatatcc accactcatc gaaactaata acacaatgat acctgtaatc
      121 gtatatatga taaaataagt taaaagccca tacccagcac cacgcctaat gtttccacca
      181 ccgatggcaa aggcgatgat taaaccgata agccccaata agaaacctaa cgcaaagcca
      241 ccaccgaaac tgctattgtc aatatcgttg ttcatttttc tacctctttc ttttcacttt
      301 ttatataact tgtaattaac gctgatgatg taattagaaa cgcaacggtg cttataacaa
      361 cccaattcag catacaaacc tcgatgtcta attcaatacc gccaataaga taagttagtg
      421 caaaagttag tgcgataaca ataaagaaga atgtatatat tactaaaaac gatataaccg
      481 ttagatatac acaatcaaat attttttcta atgttttcat aattttcctt tcttcatcat
      541 ttttctttgc ttgatgatag caattttaat tttgattttt ctaaatagta atcgaacttt
      601 gaacttaacc ttgtgccaat cgcttttggc ttgatcatac atatcgtcga gttgctttct
      661 agtctttggg tggttcttaa atagcctggt caggatgtag atagcaaagg ggatggtgat
      721 caacttttcc ggtgtgatcg gtatgtatag aaaggcaata taactgcgac taaaccacac
      781 taaccaatcg ggcaatctca tcggtataaa ggcgaataca taccacatac catttgtgac
      841 tatccaagca agaccaaacg atattagaaa tcgccaatta gtgaatggct ttaagtattt
      901 ccatagccac aatataaagc gtttcactta atcacctcta tctcgaataa gtcaatttgg
      961 acatactcgt tttctttgta gtgttttata cgagcgttag caatctcgac ataatcagca
     1021 ttgatttcac aagcagtcca attt
//
LOCUS       DAOSEX010000335         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_154956_length_1040_cov_3.600000, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000335 DAOSEX010000000
VERSION     DAOSEX010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154956_length_1040_cov_3.600000"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..>1040)
                     /locus_tag="PK612_05195"
     CDS             complement(252..>1040)
                     /locus_tag="PK612_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOH95536.1"
                     /translation="SPGVGQTVMLKASENLTPVVLELGGKSPLIIAEDADIPLTIRRL
                     KFAKLINCGQTCVAPDYVLLDKKLIDKVDDIRQAFSVEGEEKARLPKIINEKHYLRLK
                     GVLTEVGSDNFDEETQTINPTVVLNPPLDSELMREEIFGPILPIITYETLDEAIAFIN
                     ERPKPLALYLFTKSKEIMNKVLRETTSGSVAINDAVVQLANPHFPFGGVGNSGMGSYH
                     GYHTFKTFSHMKAVLHKSRAFDFRFRYKPGPKTVGIFKKLTRNR"
BASE COUNT          322 a          214 c          164 g          340 t
ORIGIN      
        1 agcctttcaa agcctcctcc ttctgctcat ctgaataaac atattcaact tttttagggt
       61 cgagaacaac aagttcgcgt tcaagactca tacttttata gccaggaatt ttccgccgac
      121 ctacagtttg gaataacttc atcacttttc cctccatcgc gcatattata cacgcgtaaa
      181 gagcaaaaag taaagtaaat gtgtaaatag ttcactttta agtgaaacga ccataaataa
      241 tgaatgtatg tttatctatt tctcgttaat ttcttaaaaa ttccaactgt ttttggacca
      301 ggtttatatc taaaacgaaa atcaaaagca cgggacttat gtaaaacagc cttcatatga
      361 ctaaatgttt taaaagtatg atacccgtga taactcccca tcccgctatt gccaacacca
      421 ccaaagggga agtgcggatt tgctagttgc acaaccgcat cattaatcgc gacagaacca
      481 cttgttgtct ctcttagaac tttattcatt atttctttcg atttagtgaa aagataaagc
      541 gctagcggtt taggacgttc attaataaaa gcaatcgctt catcaagtgt ttcataagta
      601 ataatcggta aaattggacc aaatatttct tcacgcatta gttcgctatc aagtggtgga
      661 tttaacacaa ctgttgggtt aatagtttgt gtttcttcat caaaattatc actacctact
      721 tcagttaaaa cacctttaag tcgcaagtaa tgcttttcat taataatttt aggtaacctt
      781 gctttttctt caccttcaac actaaatgct tgacgaatat catcaacttt atcaataagt
      841 tttttatcaa gtaacacata gtcaggcgct acacatgttt gtccgcaatt aataagttta
      901 gcaaatttaa ggcgccgaat cgttaagggg atatcagcgt cttctgcaat tattaacggt
      961 gactttccgc ctaattcaag gacgactggt gttaaatttt cacttgcttt aagcatcacc
     1021 gtttgtccga ctccaggact
//
LOCUS       DAOSEX010000336         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_156652_length_1033_cov_2.504090, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000336 DAOSEX010000000
VERSION     DAOSEX010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156652_length_1033_cov_2.504090"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /gene="ruvB"
                     /locus_tag="PK612_05200"
     CDS             complement(<1..775)
                     /gene="ruvB"
                     /locus_tag="PK612_05200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003773741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOH95537.1"
                     /translation="MSNRLVSDKLDQSEASVELSLRPLTLNEYVGQTALKHNLNIFIT
                     AAKARNEVLDHTLLFGPPGLGKTTLAHIIANEMGANIKVINGPSIEKAGDLAAILTTL
                     EAGDVLFIDEIHRLPRVVEEVLYSAMEDFKLQVMITLDGNVKNLQVDLPPFTLVGATT
                     RAGDLSSPLRARFGISEKIDYYEESDIFNIIKRTSRVFELPINDDAALEIARRSRRTP
                     RIANRLFRRIRDFATFASKRVIEYELCLEALKHLRVDTLG"
     gene            complement(775..>1033)
                     /locus_tag="PK612_05205"
     CDS             complement(775..>1033)
                     /locus_tag="PK612_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOH95538.1"
                     /translation="SEELSYLKKLPGVGPKGASQIILDLKGKLQTDTSKGLNPYEQEV
                     TLGLQSLGFKKSEITKVLKKVSNKSLDANELLRLALSELRR"
BASE COUNT          299 a          226 c          171 g          337 t
ORIGIN      
        1 gcccaagcgt atcaacacgt aaatgtttta aagcttcaag acatagttcg tattcaatca
       61 ctcgcttact agcgaaagtc gcaaagtcac gaatgcggcg aaagagccga ttagcaatgc
      121 gcggtgttcg tctgcttctg cgtgcaattt caagcgcagc gtcatcatta atcggtaatt
      181 caaacacacg acttgtccgt ttaataatgt taaaaatatc actttcttcg taataatcaa
      241 ttttttcact aatgccaaag cgtgcccgga gcggactact taagtcaccg gctcttgttg
      301 ttgcaccaac aagcgtaaag ggtggtaaat caacttgtaa atttttaaca ttaccatcaa
      361 gcgtaatcat cacttgtaac ttaaagtctt ccattgcact atataagact tcttcaacga
      421 cacgcggtaa acggtgaatt tcatcgataa ataacacatc acctgcttct aaagtcgtta
      481 aaatcgctgc taagtcaccg gctttttcaa tacttggacc attgattact ttaatatttg
      541 cgcccatttc attagcgata atgtgcgcta atgttgtttt tccaagtcct ggcggtccga
      601 aaagtaaagt atgatcaagt acttcattcc gtgctttagc ggcagtgata aaaatattta
      661 aattatgttt aagcgcagtt tgcccgacat attcattcaa tgttaacggg cggagactta
      721 attccacact tgcttcactt tgatctaatt tatcactcac taatctgtta ctcattaccg
      781 tcttaactca ctaagcgcta gacgtaataa ctcattagcg tcaagcgact tattactaac
      841 tttctttaaa actttagtaa tttcactttt cttaaaccct aagctttgta atccaagcgt
      901 tacttcttgt tcataaggat ttaagccttt tgaagtgtca gtttgtaatt taccctttaa
      961 gtcgagaata atttgactcg cgccttttgg accaacgccc ggtaatttct ttaagtaact
     1021 aagttcttca ctc
//
LOCUS       DAOSEX010000337         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_157641_length_1029_cov_2.378850, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000337 DAOSEX010000000
VERSION     DAOSEX010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157641_length_1029_cov_2.378850"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1029)
                     /locus_tag="PK612_05210"
     CDS             complement(<1..>1029)
                     /locus_tag="PK612_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOH95539.1"
                     /translation="LKDWLSENDIDAPSLGKKEVIELLKDTTDENVQEVLLLRQLISK
                     SSVKKYEAIINSKCKDNRVRGMFQFYGANRTGRFSSKIVQLQNLPRNSLEDLEAARAL
                     VKANNLPALEMLYGDVTDVLSQLIRTAFIPAPGHKFIVSDFSAIEARVIAWYAEEEWR
                     LEAFRNNEDIYCASASQMFGVPVLKNGVNSDLRPKGKIAELALGYGGAVGALVAMGAI
                     DYGLKEEELQALVNAWRSTNPRIVEFWWKVDKAIKKVINEGKVIRLGKLIFEYRAKML
                     FIHLPSGRKLSYVKAEIREGQITYYGIGDNKKFVRIESYGPKFVENIVQATARDLLVF
                     AMKNMNLP"
BASE COUNT          294 a          249 c          169 g          317 t
ORIGIN      
        1 atcggtaagt tcatattttt catcgcaaat acgagtaagt cccgagctgt cgcttgcaca
       61 atgttttcga caaactttgg gccatacgac tcaatacgaa caaacttctt attgtcgcca
      121 atcccgtaat aagtgatttg tccctctctt atttccgctt taacgtatga gagtttccta
      181 ccacttggca aatggataaa aagcatcttg gcacgatact cgaatattaa ctttccaagt
      241 cttattactt tgccttcgtt aattaccttt ttaatggctt tatctacttt ccaccaaaat
      301 tcaacgatac gaggattagt cgaccgccag gcgtttacaa gcgcttgtaa ctcttcttct
      361 ttaagaccgt aatcaatcgc ccccattgct actaacgccc ccacagcgcc cccataaccg
      421 agcgctaact cagcaatctt ccctttagga cgaaggtcac tattgacacc attctttagg
      481 acgggaacac caaacatctg actcgcacta gcgcaataaa tatcttcgtt attccgaaac
      541 gcttctaagc gccattcttc ttcggcgtac cacgcaataa ctcgtgcttc aatcgctgag
      601 aagtcgctaa cgataaactt atggccagga gcaggaatga atgctgttct aataagctgc
      661 gataaaacat cagtaacgtc accataaagc atttccagtg ctggtagatt atttgcttta
      721 actaaagcac gagcagcctc taagtcttca agcgagtttc gtggtaagtt ttgtagttgc
      781 acgattttac tagagaaacg acccgtacga tttgcaccat agaactgaaa cataccgcgc
      841 accctgttat ccttacattt agaattaata atcgcttcat actttttaac ggatgacttt
      901 gaaattaatt ggcgtaaaag taatacttct tgcacatttt catcagtcgt atctttaagt
      961 aattcgatta cttctttctt acctaatgaa ggagcatcaa tatcattttc acttaaccaa
     1021 tcttttagt
//
LOCUS       DAOSEX010000338         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_158365_length_1026_cov_2.153450, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000338 DAOSEX010000000
VERSION     DAOSEX010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158365_length_1026_cov_2.153450"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1019)
                     /locus_tag="PK612_05215"
     CDS             complement(126..1019)
                     /locus_tag="PK612_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008858593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOH95540.1"
                     /translation="MRYYTRLEYQIVAELSYKYEITKLCKVMQVNRSGFYKWQRRVTC
                     PHERDLKRAKDIGMFLTYHAAYPSHGYRWLNAKIRLDTGLIISDKYAHKCCKYAGIRS
                     KSKRYRYKKPGEEGRSYPNLVLQNLAITKPYEVIVSDMTAMYIQNIYHELTLYMDLYN
                     NEIIGYALTNNRGNPKTYYEGLQMVLEKKKEYPHLETILHSDQGSVYSSKSYNELLPN
                     YNITRSMSRVGTPTDNGAMEAIVGWVKEELFLDFQLNQSDNIIKTVDDYINYFNNERP
                     ACSLNYLTPVQFKERRLAKDN"
BASE COUNT          291 a          199 c          174 g          362 t
ORIGIN      
        1 gcactaacaa ttattattat tctattaatt aatttattat cgacttttag gtattttaaa
       61 aatataaata aaaataatat tgcataagtc aactaaaagt agacacctat aattataagt
      121 aaactttaat tgtcttttgc tagtctcctt tctttaaatt ggaccggtgt taagtaatta
      181 agtgaacacg ctggtcgttc attgttgaag taatttatat aatcatcaac tgttttgatg
      241 atattatcac tttgattaag ttggaagtcg aggaacaatt cttccttgac ccacccaaca
      301 attgcttcca tcgcaccatt gtccgttgga gttccaacac gcgacattga tcgcgtaatg
      361 ttataattag gtagaagctc gttatagctt ttggatgagt acacactccc ttgatcacta
      421 tgcaagattg tctcaagatg tgggtactct ttctttttct ctaagaccat ttgtaacccc
      481 tcataatacg ttttgggatt acctcggtta tttgttaagg cgtaaccaat aatttcatta
      541 ttatagaggt ccatataaag ggttaattca tgataaatat tttgaatata catcgcggtc
      601 atatcactta caattacttc atatggtttt gtaattgcta aattttgtaa gacaaggttg
      661 ggatagcttc gtccttcctc accaggcttc ttataacggt atcgtttgga tttggatcgg
      721 attcccgcat atttacagca tttatgcgcg tatttgtcac ttatgatgag tccggtgtct
      781 aatctgatct tcgcattcaa ccaccgatac ccgtgacttg gatatgccgc atgataagtt
      841 agaaacatcc caatatcctt cgcgcgttta agatcccgtt cgtggggaca agtcacccgc
      901 cgctgccact tgtagaagcc gctacgattt acttgcatca ccttacataa cttggtgatc
      961 tcatacttat aagaaagctc cgcgacaatt tgatattcta atcgggtata atatcgtatt
     1021 cctttt
//
LOCUS       DAOSEX010000339         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_159054_length_1023_cov_2.679752, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000339 DAOSEX010000000
VERSION     DAOSEX010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159054_length_1023_cov_2.679752"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PK612_05220"
     CDS             complement(<1..450)
                     /locus_tag="PK612_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95541.1"
                     /translation="MDIKISTLEISASAVKLVVGYELEGSPYVLYANKVALSQAISAG
                     EIVAPDQVINAIKVLAADAREALGETISEVVLALPTIDFEVFRGTQTTNTLSNKIDRI
                     DIKNIMALFRKNRVDPNKVIVAILPQVFKTDSDQVYRLLPLGEVSNTL"
     gene            complement(606..824)
                     /locus_tag="PK612_05225"
     CDS             complement(606..824)
                     /locus_tag="PK612_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95542.1"
                     /translation="MRDKLYKTNKKVGYYRFRAFLIGILLVAVGAAALTIPYPYVAKA
                     INEAQARRAQAEEESSEEAPLVSEDLTI"
     gene            complement(824..>1023)
                     /gene="mraW"
                     /locus_tag="PK612_05230"
     CDS             complement(824..>1023)
                     /gene="mraW"
                     /locus_tag="PK612_05230"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HOH95543.1"
                     /translation="VKNVFKKLATLSDEGLHPYTLPDKNRVAPFTLVNRKVIVATDEE
                     IELNPRASSAKLRIIEKRRTK"
BASE COUNT          297 a          202 c          187 g          337 t
ORIGIN      
        1 tagcgtatta cttacttcac caagtgggag taagcgatac acttgatcgc tatctgtttt
       61 aaaaacttgc ggtaaaatcg ccacaattac tttatttgga tcaacacggt ttttacggaa
      121 aagtgccatg atatttttaa tatcaatccg atcaatttta ttcgaaagcg tgtttgtcgt
      181 ttgtgttcct ctaaacactt caaaatcaat cgttggtaag gctagcacta cttcactaat
      241 cgtttcgcct aatgcttcac gagcgtccgc tgctaaaact ttaatcgcgt taatgacttg
      301 gtcaggcgcg actatttctc ccgcacttat tgcttgtgaa agcgccactt tatttgcata
      361 aagcacatag ggactacctt caagttcata accgacaacg agtttgacgg cactggcact
      421 aatttctaat gtactaattt taatatccat aattctccaa taatgtaatt attttacaat
      481 aattaacgaa taataacaat tgcaactaat taatatttgc ttaattatca caaatttaag
      541 aaaaaagcct tagtgagaga ggaaaagggg aagagttcac taaggcttta aagaaaggaa
      601 aaaaactaaa tggttaaatc ttcactaact aaaggagctt cttcactgct ttcttcttcg
      661 gcctgagcac ggcgtgcttg tgcctcgtta atcgctttag cgacatacgg ataaggaatc
      721 gttaaagctg ccgctccaac cgctacaagt aaaatcccta taagaaaggc tctaaagcgg
      781 tagtatccga cttttttatt cgttttgtat aatttgtcac gcattattta gtcctccttt
      841 tttcgatgat tcgcagtttt gcactactag cgcgtgggtt gagttctatt tcttcgtcag
      901 tggcgacgat gactttacga ttgacaagtg tgaagggtgc tactctattt ttatcaggta
      961 gtgtgtaagg atgcagtcct tcgtcactca gtgtcgctaa ctttttaaag acatttttta
     1021 cta
//
LOCUS       DAOSEX010000340         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_159827_length_1020_cov_2.850777, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000340 DAOSEX010000000
VERSION     DAOSEX010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159827_length_1020_cov_2.850777"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..873)
                     /locus_tag="PK612_05235"
                     /pseudo
     CDS             complement(1..873)
                     /locus_tag="PK612_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(930..>1020)
                     /locus_tag="PK612_05240"
     CDS             complement(930..>1020)
                     /locus_tag="PK612_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010772099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH95544.1"
                     /translation="VLISIPITILFVFMQRYYVSGVTGGAVKG"
BASE COUNT          304 a          220 c          191 g          305 t
ORIGIN      
        1 cgaaagttgc ggaccagagc gcgccataat gctgtcaatt ataaacccag tcgcgttagc
       61 gccatcaaaa ttaaagagtg attcaacccc ggcgtagtaa tacttttgca cattaaagtt
      121 ttcccatgct tcagcgacga tataactatc gggtttataa cttttaacca tatcgctaaa
      181 ccaccctaaa aagtcgatgt tttccggatg agtttcaaag aaatgacccg cagcgtcaag
      241 tctaaaccct ttaaccccgc gatcaagcca aaatttacta atgctcgtaa tttcttcacg
      301 tacggctgta ttgttgagta gtagttcagg catatggtcg ccaaagtacc caattttaac
      361 ttttacttca ccattttcaa tctaaaagtc ataccagtta cccttatcac tggggtcgtt
      421 aggattgtat tcaggtgtat taaaacgcct tcgaccttca ataaaccacg ggtgcgtacg
      481 cgccgtgtga ttaataacga ggtcaataat aacatcaata ttatgttcag ctgcgacact
      541 gattaaagta tcaaagtctt caagcgtacc aaagtcagga tgaatcgctt tataatcggt
      601 tacatcatag ccatgataac taggtgaagg gttaatcggg gttaaccaaa tacccttcac
      661 tcctagttct tgtaaatatg gaattttatc agtgatacct tttaagtcac cgtggccatc
      721 cgcattagtg tcggcaaatg agcgcacgaa aatttgatag tatacgccgc ctttttcatt
      781 agtgattgac tcatcaattt cataatccgt cggcgcttgt agtatttctt cacttgaaac
      841 actttcgctt ggtaaatctt tacaacttgc cataaatagt aaggggacga taagcattaa
      901 agcacgaagg ctttgttttg ctttcatagt tatcccttaa ctgctccacc tgttacaccg
      961 ctgacataat agcgttgcat gaagacgaag agaattgtaa ttggaattga aatgagtact
//
LOCUS       DAOSEX010000341         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_159845_length_1020_cov_2.625907, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000341 DAOSEX010000000
VERSION     DAOSEX010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159845_length_1020_cov_2.625907"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1020
                     /locus_tag="PK612_05245"
     CDS             <1..>1020
                     /locus_tag="PK612_05245"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOH95545.1"
                     /translation="KPFITPFAALTFSDKGENGLSQNFHRFVIDHVVRYEYARKPRPI
                     LLNNWEATYLTFDEAKIKKLIREASKLGIELMVLDDGWFINRDDDFGGLGNYEVNKKK
                     LPRGLKGLSDYATKLGMSFGLWFEPEMVSADHPIFTEHPEWVVKIPGVKPSTGRHQYS
                     LNLTLKEVQDYIVENVSKVLKSADIKYVKWDYNRNLSDYYSPSGEYGTFFYDYTLGLY
                     AIMQRLTKEFPTILWEGCASGGNRFDLGILSFFDQIWVSDCSDAYERIRMHTNLAKAY
                     PLSVMSGHVSAAPNHQLLRDLPLQTRFNNAFMSGGFGYELDVNNLTPKDKKEIKAYNE
                     IAKAF"
BASE COUNT          320 a          171 c          214 g          315 t
ORIGIN      
        1 gaaagccttt tattaccccg tttgccgccc ttacatttag tgataaaggc gaaaatggcc
       61 ttagtcaaaa cttccatcgc tttgttatag accatgtcgt gcgttatgaa tatgcgcgga
      121 aaccgcgtcc aatcttactt aataactggg aagcaactta tcttacattt gatgaagcaa
      181 aaattaaaaa actaatacgt gaagcaagta aattagggat tgaactaatg gttttagatg
      241 acggatggtt tatcaatcgt gatgatgact ttggcggatt aggtaattac gaagtaaata
      301 agaagaagtt accacgcggc ttaaaaggcc taagtgacta cgcaacgaaa ttaggaatgt
      361 cctttggttt atggtttgaa ccagaaatgg ttagtgctga tcacccgata tttaccgaac
      421 accctgaatg ggttgttaaa attcctggtg ttaaacctag tactggacgc catcagtatt
      481 cccttaattt aacgctcaaa gaagtccaag actatattgt cgaaaatgtg agtaaagtat
      541 taaagagtgc cgatatcaaa tatgttaagt gggattataa tcgtaactta agtgattact
      601 attcaccatc gggtgaatat ggcacattct tttatgatta tacgcttggc ttatatgcga
      661 ttatgcagcg gttaaccaaa gaatttccaa cgattttatg ggaaggttgt gcaagtggcg
      721 gtaatcgttt tgatttagga attttatcgt tctttgatca aatttgggtg agtgactgta
      781 gtgatgctta tgaacggatt agaatgcata ccaatctcgc gaaagcttat ccgcttagtg
      841 tcatgtctgg gcatgttagt gctgccccta atcatcagtt actacgtgat ttaccactgc
      901 aaacacgctt taataatgcc tttatgtctg gtggttttgg ctatgaatta gatgtgaata
      961 atttaacgcc aaaagacaaa aaagaaatta aagcatataa cgaaattgct aaagcattcc
//
LOCUS       DAOSEX010000342         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_160650_length_1017_cov_1.846154, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000342 DAOSEX010000000
VERSION     DAOSEX010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160650_length_1017_cov_1.846154"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PK612_05250"
     CDS             complement(<1..387)
                     /locus_tag="PK612_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95546.1"
                     /translation="MTENKSKFKVFLAKLPKLSKKQIITIAVLLVLYVLLAIFTSDNM
                     YNLLTLTIYSAIPLAIVAMGGMYSERSGVVNIALEGTMMFGAFVGILCLSHIQHAGID
                     GQHIIFLELLIGAISGVIFSALHAFAS"
     gene            complement(374..>1017)
                     /locus_tag="PK612_05255"
     CDS             complement(374..>1017)
                     /locus_tag="PK612_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH95547.1"
                     /translation="LPTLGLDKLFNFPRLNSGIVIAIITTIIIFVLLEKTTFGYQLKA
                     VGFNREAARYAGINDKRNIVLSLVIAGALAGLAGAVIYLVPAQGERLATKHVISNYGF
                     QGIAVALLGMSHPIGTMGAGLFFGYIEAANLPLQRVGFKREIIDIISASIIYFSAFTL
                     FFQIYARRFINKMRDFFAKIKKKPQLATAEGVEIETLPPNEEGKEGGQEDDRK"
BASE COUNT          317 a          212 c          193 g          295 t
ORIGIN      
        1 tgaggcaaaa gcgtgtaagg cggagaatat aacgccagaa atagccccaa ttaagagctc
       61 aaggaaaata atatgttggc catcaattcc cgcatgttgg atatgactta agcataatat
      121 tccgacaaat gccccaaaca tcattgtccc ttcaagggca atattaacga cgcctgaacg
      181 ctcactatac atgccgccca tcgccacaat tgctaagggg attgccgagt aaatcgtcag
      241 tgttaaaaga ttgtacatat tatcactcgt aaagatagca agtaacacat aaaggacaag
      301 taaaactgcg attgtaataa tttgtttttt cgaaagtttc ggaagtttag caagaaaaac
      361 tttgaacttt gatttatttt cggtcatctt cttgacctcc ttctttacct tcttcattcg
      421 gtggtagcgt ttcaatttct actccttcgg cagttgctaa ttgtggtttt ttctttatct
      481 tagcgaagaa atcacgcatt ttattaataa agcgtcgtgc gtaaatttgg aagaataacg
      541 taaaggcact aaagtaaata atacttgccg aaataatatc aataatttcg cgtttaaacc
      601 caactctttg gagtggtaag ttcgccgctt caatataacc aaagaagagc cccgctccca
      661 tcgttcctat tggatggctc atcccaagga gtgcaacggc aatcccttgg aagccgtaat
      721 ttgagataac gtgttttgtg gcaagtcttt cgccttgcgc tggcactaag taaataacag
      781 cccccgctaa accagcaaga gcgccagcaa ttacaagtga gagcacgata ttacgtttat
      841 cattaatccc tgcataacgc gcggcttcac ggttaaagcc aactgcttta agctgataac
      901 caaaggttgt tttttcaagt aacacaaaaa tgataattgt cgtaattatc gctattacta
      961 tcccgctatt aaggcgcgga aagttaaata atttatcaag tcctaatgtt ggtaatt
//
LOCUS       DAOSEX010000343         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_161292_length_1014_cov_2.908238, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000343 DAOSEX010000000
VERSION     DAOSEX010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161292_length_1014_cov_2.908238"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PK612_05260"
     CDS             <1..295
                     /locus_tag="PK612_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95548.1"
                     /translation="MTRFTAICSKYHKESVIFLEYYLAQDDDPVIKDFKNLIFETVKA
                     HIPKVTPPMFELLYHFIATGTVFVLRKSAPENIVANLRTLYIFLFKDINERVI"
     gene            298..>1014
                     /locus_tag="PK612_05265"
     CDS             298..>1014
                     /locus_tag="PK612_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOH95549.1"
                     /translation="MKKIAAFIVKFKVFFFGLWIALTIASIFMIPHVKINYDTTKYLP
                     EDTRVKESLRVMEEEFGITGQASVMIEKVETLQEVFVYKDEIKRIPTVMEVVWIDSFV
                     ERDTINDIQRIAEENGMSGVFDFANIPGLNAFYKDKSALLQIVFTEEDHSRAVGKAIE
                     QIRSYLEGIKRPYAMRGTAVNAYYTQKLTESEVLRVTVIVVPIILFILLVFTRSWAEP
                     IIFLIAVGVAVLVNMGTNLFF"
BASE COUNT          310 a          163 c          209 g          332 t
ORIGIN      
        1 tatgacacgt tttacagcga tatgttcgaa atatcataaa gagtcagtaa tctttcttga
       61 atactattta gcgcaggatg atgatcccgt aattaaagat tttaaaaatc ttatttttga
      121 aacagtgaaa gcgcacattc ccaaagttac accgccgatg tttgaattgc tctatcattt
      181 tattgcaaca ggtactgtct ttgtattacg gaaatcagcc cctgaaaaca ttgtcgccaa
      241 tttaaggaca ctttatatat tcctatttaa agacattaat gaaagggtca tttagtgatg
      301 aagaaaatcg ctgcttttat tgttaaattt aaagtattct tctttgggtt atggattgcc
      361 ttaaccatcg ctagtatttt tatgattccc catgtcaaaa taaattacga cacgacaaaa
      421 tatttgccag aagatacgcg tgttaaagaa tctttacgtg tgatggaaga agagtttggc
      481 attactggtc aagctagtgt aatgattgaa aaagttgaaa cattacaaga agtgttcgtg
      541 tataaagatg aaattaaaag aattccaacg gtgatggaag ttgtgtggat tgatagtttc
      601 gtcgaacgcg atacgattaa tgatattcaa cgcattgctg aagaaaatgg gatgtccggt
      661 gtctttgact tcgcaaatat tccggggctt aacgcttttt ataaagataa aagtgctttg
      721 ctgcaaattg tgtttacgga agaagatcat agtcgcgctg tcggcaaggc cattgaacaa
      781 atccgctcct atcttgaggg tatcaagcga ccttatgcaa tgcgcggaac agcagttaat
      841 gcttattaca cgcagaagtt aactgaatcg gaagtattgc gggtaacggt aattgttgtc
      901 ccaatcatct tatttatttt attagtgttt accagaagtt gggccgagcc tattatcttt
      961 ttaattgccg tcggggtcgc tgttttggta aatatgggta caaacttatt tttc
//
LOCUS       DAOSEX010000344         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_161368_length_1014_cov_2.241919, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000344 DAOSEX010000000
VERSION     DAOSEX010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161368_length_1014_cov_2.241919"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..409)
                     /locus_tag="PK612_05270"
     CDS             complement(146..409)
                     /locus_tag="PK612_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95550.1"
                     /translation="MLTSVFIVGTLGKNENDYRYLLVEKVPGLDYEDDEERAKYDYFK
                     VKHWSNTPSAFNRLAEGREVALKGRLEEIEGETYIIAELYREF"
     gene            complement(429..>1014)
                     /locus_tag="PK612_05275"
     CDS             complement(429..>1014)
                     /locus_tag="PK612_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95551.1"
                     /translation="HDNGVLVDKMTVSNASECKETHLARLLSMNTIVMLSFGVPFFHR
                     GQEIGVSKYGDLNSYKSSDKVNQFPYTLALKRKEMVEYFKALTALRKDCKFLHETDPK
                     VIKNMIEFKDLPGGASQIVYNPDMDLTPYSHFNVYVNPTSEPVFISLEKPLKTILSSG
                     GYIAARSKEYFERTMVAPYSVIVLGLRKGDSGPK"
BASE COUNT          300 a          217 c          163 g          334 t
ORIGIN      
        1 caagtttttg tccttttgcc atccgcaatt cgggagagta cttatcagga cgccgtgcgt
       61 aggggcgcat ccacgtaaaa taaccccaca ttaaggggat tgctaccttc actatccaaa
      121 taatatactt aatcataatc tctttttaaa actcacgata taattcggca ataatatacg
      181 tttctccttc aatttcttcg agtcgacctt ttagtgcaac ttcacgtcct tcggcaagcc
      241 tattaaaggc tgaaggtgta ttcgaccaat gtttgacttt aaagtagtca tactttgctc
      301 tttcttcatc atcctcataa tctaaccctg gcactttctc gacaagtaaa tagcgataat
      361 cattttcgtt tttacctagg gtaccaacga taaatacgga tgttaacata atccttcctc
      421 ctattagttt attttgggcc agaatctcct ttccgtagtc ctagcacaat tacactatag
      481 ggggcaacca tcgttctttc gaaatattct ttcgagcgag cggcgatata tccgccacta
      541 cttaaaattg ttttaagcgg tttttctaaa ctaataaaaa ctggttcact agtgggatta
      601 acataaacat taaagtgact atatggggtt aaatccatat cgggattgta cacgatttga
      661 cttgcgccac ctggtaaatc tttaaattca atcatattct taatgacttt aggatcagtt
      721 tcatgtaaaa atttacaatc tttgcgtagc gccgttaaag ctttaaaata ttcaaccatt
      781 tctttacgtt tcaaggctaa agtatacggg aattggttaa ctttatcgct gctcttatat
      841 gaatttaagt caccatattt tgacacacca atttcttgtc cgcgatggaa gaaaggcaca
      901 ccaaaagaga gcatgacaat cgtattcata ctaaggagtc tcgctaaatg agtttcctta
      961 cattcactag cgttactaac cgtcatttta tcaactaaaa caccgttatc atga
//
LOCUS       DAOSEX010000345         1009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_162591_length_1009_cov_2.199161, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000345 DAOSEX010000000
VERSION     DAOSEX010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162591_length_1009_cov_2.199161"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1009
                     /locus_tag="PK612_05280"
     CDS             <1..>1009
                     /locus_tag="PK612_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH95552.1"
                     /translation="SILKYAFNKRGKDVFTMIPSRYKDGYGLNTAVVDKFSNRGINLI
                     ITVDNGINQYEAIDLANALGIDVLVSDHHELGEALPNAVAILHPELSNLPRINSCGAY
                     MALVISYGILGYYDDYLISLAGIATLADMMPLVDRNRTVVKLAIEAMNDQQYFTLIKL
                     NDSTFVDETSMSMKIAPKINALGRLSQNYEANVLVEYFTTTDIKRHYDISAYVEKVYR
                     ERKDMSRGETLSGEELAFPAIVLITDELEGVLGLIAQNYVLEHNKPAFVFTESSEDPA
                     LLKGSVRSRSGFNVVDAFSHLNHLIVTSGGHPEAGGLTIKKDDFKAFYHAVNEYAKHH
                     PL"
BASE COUNT          304 a          174 c          218 g          313 t
ORIGIN      
        1 caagtatctt aaaatatgct tttaataagc gcggtaaaga cgtgtttacg atgattccta
       61 gtcgttataa ggatggctat ggcttaaaca ctgcggttgt cgataaattt agtaatcgcg
      121 gcattaattt aatcattact gttgataacg gcattaatca atatgaagca attgatcttg
      181 ctaacgcttt aggaattgat gtccttgtta gcgatcatca tgagcttggt gaagccttac
      241 ctaatgctgt tgcgattctc caccctgaac taagtaattt accgcgtatt aatagttgcg
      301 gggcatatat ggcacttgtg ataagttacg gaatattagg atattatgat gattatttaa
      361 ttagtcttgc ggggattgcc acgcttgccg atatgatgcc gcttgttgac aggaatcgga
      421 cagtggtaaa gcttgctatt gaagcaatga atgaccagca atatttcacg ctcattaaat
      481 taaatgatag tacgttcgtt gatgaaacat caatgagtat gaaaattgcc ccgaaaatta
      541 atgccctagg ccgcttaagt caaaattatg aggcgaatgt gttagtggaa tactttacga
      601 cgactgacat taagcgtcat tatgatatca gtgcttacgt tgaaaaagtg tatagagaac
      661 ggaaagatat gagccgtggt gaaacgttaa gtggcgaaga acttgccttt cccgcgattg
      721 ttttaataac cgatgaatta gaaggggtct taggcttaat tgcccaaaat tacgtcttag
      781 aacataataa accagccttt gtctttaccg aaagtagtga agatcctgct ttacttaaag
      841 gtagtgtccg cagtcgcagt ggctttaacg ttgtcgacgc ctttagtcac ctaaatcatc
      901 ttattgtcac aagtggtggt catcccgagg ctggtggttt aacaattaaa aaagatgatt
      961 ttaaagcttt ttaccatgca gttaatgaat acgctaaaca tcatccact
//
LOCUS       DAOSEX010000346         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_163306_length_1006_cov_2.977918, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000346 DAOSEX010000000
VERSION     DAOSEX010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163306_length_1006_cov_2.977918"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1006)
                     /gene="tgt"
                     /locus_tag="PK612_05285"
     CDS             complement(<1..>1006)
                     /gene="tgt"
                     /locus_tag="PK612_05285"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOH95553.1"
                     /translation="VGTLANVKTITPEEIVALGSGVILANTYHLSLRPGTDILKKAGG
                     IHSFMNHHGPILTDSGGFQVFSLADNRKISEEGVTFKSHLDGRELFFSPEEAIRMQEE
                     IGADIIMSFDECIHYPADYEYVKASTERTLRWAKRGKAAHKREDQALFGIVQGGEYPD
                     LRKYSAMETVKIGFPGYAIGGTSIGEPKDVFIKMVESSVPYLPKDKPRYLMGIGSIDM
                     ILQGVARGVDMFDCVLPTRIARHGSLYTRKGRLNIRRAEFKDDFRPIEEECDCYTCKN
                     YTRAYLRHLYVSEEALAQRLLSIHNLRFLIKLMEDARVAIKENRFLDFKSDVLKKFGD
                     "
BASE COUNT          297 a          226 c          177 g          306 t
ORIGIN      
        1 ttgtcaccaa acttttttaa gacatcactc ttaaaatcta aaaagcgatt ttctttaatc
       61 gccacacgcg catcttccat aagtttaatt aaaaagcgta agttatgaat tgagagtaag
      121 cgctgcgcta aagcttcttc actaacatat aaatggcgta aataagcacg cgtgtaattc
      181 ttacatgtat agcaatcgca ctcttcttca attgggcgga agtcatcttt aaattcagcg
      241 cgccggatat taagtctgcc ttttcttgta tagagcgaac cgtgacgcgc aatacgcgtt
      301 ggtagtacac aatcaaacat atcaacaccg cgcgctacac cttgtaagat catatcaatt
      361 gaaccaatcc ccattaagta acgcggtttg tctttaggta aataagggac ggaactttcc
      421 accatcttta taaagacatc ttttggttca ccgatgcttg ttccaccaat tgcgtaacca
      481 ggaaacccaa tttttactgt ttccatcgca ctatatttgc gtaagtcggg gtattctccg
      541 ccttgtacaa taccaaataa agcttggtct tcacgcttat gcgctgcttt tccgcgctta
      601 gcccagcgta aagtacgttc agtacttgct tttacatatt cataatctgc gggataatga
      661 atacattcat caaaactcat aataatatcc gcaccaattt cttcttgcat cctaattgct
      721 tcttctggtg agaagaaaag ttcgcgtcca tctaaatgtg atttaaaggt cacaccttct
      781 tcgctaatct tacgattatc ggctaaagag aatacttgaa agccgcctga atcagtaaga
      841 attggtccgt gatgattcat aaaagagtgg attccaccag ccttttttaa aatatcagtg
      901 cctggtctaa gtgataagtg ataagtattc gccaagatga ctccgctgcc taaggcaaca
      961 atttcttcag gagtaattgt tttaacatta gcaagcgtac ccaccg
//
LOCUS       DAOSEX010000347         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_163417_length_1006_cov_1.933754, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000347 DAOSEX010000000
VERSION     DAOSEX010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163417_length_1006_cov_1.933754"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1006
                     /locus_tag="PK612_05290"
     CDS             <1..>1006
                     /locus_tag="PK612_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95554.1"
                     /translation="WTITSAPYQTQNNVRVYVGESDQETLTHYLNFDHTTLTTANLTA
                     EHIRGALTDIFDGAGSDWSVSGSDNEVIFTNNSDVQLDAPAFDDDDFNEYSKAFVEIE
                     ITDISSVATATDWGVMLEIDGQEYWFEDNIIAGDPTDIIAQALVNSAIWTNVGGVFGD
                     STTFPSVFDITQDYDSVIITKKTDGTILPATLSLFSQTPSYNQTNFTFGRYSVLEPQS
                     HILTFTILPDNITANIDCEPIYVGDFTANQSAQCIWDELDRISDITNNFTLSYLNNVL
                     TFTAKNYGEKLITISAQPIYLSTSRNIVASGSIEVIAPDFDFTLTNHQYGEITTPLFT
                     "
BASE COUNT          322 a          206 c          185 g          293 t
ORIGIN      
        1 catggacaat tacaagcgcc ccctatcaaa cgcaaaacaa tgtaagggtt tatgtcggag
       61 aatctgatca agaaacactt actcactatc ttaatttcga ccacacaaca ttgaccactg
      121 ctaatttaac cgctgaacac attaggggcg cattaaccga tatttttgat ggcgctggct
      181 cggattggtc agtaagtgga agcgataatg aagttatctt tactaataac agcgatgtgc
      241 aattagacgc accggctttt gatgatgacg actttaacga gtattccaaa gcgtttgtcg
      301 aaattgaaat caccgatatt tcatcggtcg ccaccgctac tgattggggg gtaatgttag
      361 aaatagacgg gcaagagtat tggtttgaag ataacattat tgctggcgac ccaaccgata
      421 taatagcaca agcactcgtc aactcggcaa tttggactaa tgtgggtggt gtctttggcg
      481 acagcacaac atttccttcc gtttttgata ttacccaaga ttatgattcg gttatcatta
      541 ctaaaaaaac cgatggaaca atactgccag ccacattgag tttatttagt caaacaccat
      601 catataatca aacgaacttt acatttgggc gatattcggt attagagcca caatcccaca
      661 tattaacctt tacaatcttg ccagataata taaccgcaaa tatcgattgc gaaccgattt
      721 atgttggcga ttttaccgct aatcaaagcg cacaatgcat atgggatgaa ttagatagaa
      781 tctccgatat aacaaataat tttacattat cttatcttaa taatgtttta acctttaccg
      841 ctaaaaatta tggtgagaag ttaatcacta tttcggcaca accgatttat ttgtcaacat
      901 ccaggaacat tgtggcgagt ggaagcatcg aagttattgc gcctgatttt gatttcactt
      961 taaccaatca ccaatatggc gaaatcacaa cacctttatt cacaat
//
LOCUS       DAOSEX010000348         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_163557_length_1005_cov_2.932632, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000348 DAOSEX010000000
VERSION     DAOSEX010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163557_length_1005_cov_2.932632"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PK612_05295"
     CDS             complement(<1..528)
                     /locus_tag="PK612_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOH95555.1"
                     /translation="MPLVSMKEMLIKANKEKYAVGQFNINNLEWTQAILDEVQELQAP
                     VILGVSEGAAKYMGGYNVVVQMVKALIKDRGYTIPIAIHLDHGSSEEVCKKAIDAGFT
                     SVMIDASHHPFKENLEITKRVVDYAHARGVSVESELGSIGGQEEHVIAETLYADPNEC
                     LEMVQKTKIDCLAPAL"
     gene            complement(558..944)
                     /gene="rpoE"
                     /locus_tag="PK612_05300"
     CDS             complement(558..944)
                     /gene="rpoE"
                     /locus_tag="PK612_05300"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR04567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HOH95556.1"
                     /translation="MKKRTNIEIAYTLLKEHGESLSFYDLWQGVVNEHEYSEAEGHDL
                     ISRFYTALTMDGRFVNLGDNMWALRENVLFDDIALPLNEVYTEVDEAGDEEEEDEGPE
                     ILYFDDETGQADEAKVRSTLDEEDEE"
BASE COUNT          266 a          224 c          157 g          358 t
ORIGIN      
        1 aagcgctggc gctaagcagt caatttttgt tttttgcacc atttctaaac attcatttgg
       61 atcagcatat aatgtttcag caataacgtg ttcttcttga ccaccgatgc taccaagttc
      121 tgattcaaca ctaacgccgc gtgcatgtgc gtaatcaact acgcgtttag taatttctaa
      181 attttcttta aatgggtgat gacttgcgtc aatcatcacg gaagtaaagc cagcatcaat
      241 tgccttttta catacttctt cactgctgcc gtgatctaag tggatggcaa ttggaatcgt
      301 atagccacga tctttaatta aggcttttac catttgcacg acgacattat aaccgcccat
      361 gtatttagcg gctccttcac taactcctaa aataactgga gcttgaagct cttgtacctc
      421 atcgagaatt gcttgggtcc attctaagtt attaatgttg aattgaccaa cagcgtactt
      481 ctctttgttt gctttgataa gcatttcttt catactcact aatggcataa aaattctcct
      541 ttatctttta aaattattta ttcttcgtct tcttcgtcaa gtgtgcttct tactttcgct
      601 tcatctgctt gacctgtttc atcatcaaag taaaggattt ctggtccttc gtcttcttct
      661 tcctcatcac ctgcttcatc aacttcagtg taaacttcgt ttagtggtaa agcaatgtca
      721 tcaaataaga cattttcacg taatgcccac atattgtcgc ctaaattaac aaagcgacca
      781 tccatcgtta aggcagtgta gaagcgtgaa attaaatcat gaccttcagc ttcactatat
      841 tcatgttcgt taacaacacc ttgccataaa tcataaaatg atagtgattc accgtgttcc
      901 ttaagtaggg tgtaagcaat ttcaatgttt gtccttttct tcatatatct tcttgtatta
      961 tctaatcatg ccccggtact tcgattaggt aatacatctc ctttc
//
LOCUS       DAOSEX010000349         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_163838_length_1004_cov_2.362487, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000349 DAOSEX010000000
VERSION     DAOSEX010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163838_length_1004_cov_2.362487"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PK612_05305"
     CDS             complement(<1..598)
                     /locus_tag="PK612_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95557.1"
                     /translation="MKKAKPIKFKQKRKRWYRALKRIIKVRYKEPKFIFLGEKPTTNA
                     IILSNHVGTDAPMSLEIYAPFHLRMWGTHEMNSGLRKMYRYQSRVYYHEKKGWNLHLA
                     RLFCLLASPLTNLFYKGLRLISTYQDLRLKKTVKESLEAFKDGDNIVIFPEVSDEGYL
                     DELKGFHGGFCILAEIAYKHGIDTYLYASYFKIKEKVYL"
     gene            868..>1004
                     /locus_tag="PK612_05310"
     CDS             868..>1004
                     /locus_tag="PK612_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOH95558.1"
                     /translation="MFQNFDGGYIEVVCGPMFAGKTEELIRRIKRLEYAKQSYIIFKP
                     K"
BASE COUNT          328 a          146 c          159 g          371 t
ORIGIN      
        1 atagataaac tttttctttt attttaaagt atgatgcata taagtatgta tcaataccgt
       61 gcttataagc aatttctgct agaatgcaaa aaccaccatg aaatcctttt aattcatcta
      121 aatatccttc gtcactaact tcggggaaaa taacgatatt gtcaccatct ttaaaagctt
      181 ctaagctttc tttaactgtc tttttaagtc gtaagtcttg ataagttgaa attagtcgta
      241 gtcctttata gaagaggtta gttaaaggac ttgctaaaag acaaaaaagt cttgctaaat
      301 gtaagttcca gccctttttc tcatgataat acactcgtga ctggtaacga tacattttac
      361 gcagccctga attcatttca tgtgtccccc acatccgtaa gtggaatgga gcgtaaattt
      421 caagtgacat cggcgcatca gtgccgacat gattacttaa gataattgca tttgtcgttg
      481 gtttttcacc aagaaagata aactttggtt ctttataacg gactttgatg atacgtttta
      541 aagcgcggta ccagcgctta cgtttttgtt taaatttaat aggtttagct tttttcataa
      601 gtacccccac aagaaaatat taacatatat tcaatacaat attatttttt tatatgtttt
      661 tgacattatc tgtcaatatt cctcatgtaa gtgttttcat tttcagtgtc attttttaca
      721 attttgaaat tatgctaatg atactaaatt aattttaaaa gttgttaaat tatgtctatc
      781 tttttaatag ttaagcgttt aaaaagttgt gttaaatttg ttaagattat taatgaaaat
      841 atttgaatag agaaaagggg taagattatg tttcaaaatt ttgatggtgg ctatattgaa
      901 gtcgtatgtg gcccgatgtt tgccggtaaa accgaagaat taattagaag aattaaacgc
      961 ttagaatatg cgaaacaatc atatattatt tttaaaccaa agat
//
LOCUS       DAOSEX010000350         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_164057_length_1003_cov_2.956751, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000350 DAOSEX010000000
VERSION     DAOSEX010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164057_length_1003_cov_2.956751"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PK612_05315"
     CDS             <1..406
                     /locus_tag="PK612_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH95559.1"
                     /translation="DFANTFVNIEDMLNLIKKVKKQKYEVCLIISDTELTLPDKIYGE
                     VDYFMVNEKILTHTVNDERERLSLLSSLGKLLRHQKPIMISDLPKWSEIEYYIRAGVD
                     YVASDEISKKDNSLQTIDKKKALRIINFSKRK"
     gene            409..>1003
                     /locus_tag="PK612_05320"
     CDS             409..>1003
                     /locus_tag="PK612_05320"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801586.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOH95560.1"
                     /translation="MSKIKIDAITTQSEDFAQWYTDVCRKAELMDYSEVKGFIIYRPY
                     GYAIWENIQKYLDEKFKEKGHDNVYMPLVIPTSLFQKEKDHIEGFAPETLVATQGGNQ
                     KIEDPLIIRPTSEVLFCDHYAKIVSSYRDLPKKYNQWCSVVRWEKTTRPFLRGSEFLW
                     QEGHTIHETEVEARTHALDMLKVYENMGKELLAIPFLT"
BASE COUNT          364 a          144 c          196 g          299 t
ORIGIN      
        1 agatttcgct aacacatttg tgaacattga agatatgtta aaccttatta aaaaagttaa
       61 aaaacaaaaa tatgaagttt gtttaatcat tagtgatact gaattaacac tgccagacaa
      121 aatttatggt gaagttgatt actttatggt gaatgaaaaa atcttaaccc atacagttaa
      181 tgatgaacgg gaacgcttat ccttactttc atcattaggt aaattattac gtcatcaaaa
      241 accaataatg attagtgatt tgccaaagtg gagtgaaatc gagtattata ttcgggcagg
      301 tgtagattat gtcgctagtg atgaaattag caagaaagac aacagcttgc aaactattga
      361 taaaaagaaa gcactaagaa ttattaattt tagtaagagg aaataaagat gagtaagatt
      421 aaaattgatg caattacaac ccaaagtgaa gattttgccc aatggtacac tgatgtgtgt
      481 cgcaaggcgg aattaatgga ttatagcgaa gttaaaggtt ttattattta ccgaccttat
      541 ggttatgcga tttgggaaaa tattcaaaaa tatttagatg aaaagtttaa agaaaaaggt
      601 catgataatg tgtacatgcc ccttgttatt cccacaagtc tttttcaaaa agaaaaagat
      661 catatcgaag gttttgcccc tgaaacatta gtcgcaacac aaggtggtaa tcaaaagatt
      721 gaagatccac ttattattcg tccgacaagt gaagtattat tttgtgatca ctacgctaaa
      781 atagttagct catatcgtga tttaccaaag aaatataatc agtggtgttc tgtcgttcgc
      841 tgggagaaga caacgcgtcc ttttttacgc ggtagtgaat tcttatggca agaagggcac
      901 acgattcatg aaacggaagt agaagcacgg acccatgcgc ttgatatgct taaagtttat
      961 gaaaacatgg gtaaagaatt actcgcgatt ccgtttttaa ccg
//
LOCUS       DAOSEX010000351         1001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacilli bacterium isolate AMR_MDS_1794
            NODE_164763_length_1001_cov_1.549683, whole genome shotgun
            sequence.
ACCESSION   DAOSEX010000351 DAOSEX010000000
VERSION     DAOSEX010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863323
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacilli bacterium (wastewater metagenome)
  ORGANISM  Bacilli bacterium
            Bacteria; Bacillati; Bacillota; Bacilli.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:33:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,065
            CDSs (total)                      :: 1,035
            Genes (coding)                    :: 1,022
            CDSs (with protein)               :: 1,022
            Genes (RNA)                       :: 30
            tRNAs                             :: 28
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 9 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Bacilli bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164763_length_1001_cov_1.549683"
                     /isolate="AMR_MDS_1794"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1903720"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1001)
                     /locus_tag="PK612_05325"
     CDS             complement(<1..>1001)
                     /locus_tag="PK612_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH95561.1"
                     /translation="KGMKIIQDVVFNHTGNFGEENLFPMFEKDYTKPDTVNNLKITDP
                     YKVLPDDYATLSGDRQYGSRIDAMKEDMNDFRHIYHHEKSMGYEQYIEQIGQIAGDCV
                     DLNTENPYVTNYLIDAYNRYIDMGVDGFRVDTAKHISRLVFDKVFNPAFQARGGDNFF
                     MFAEVCTRVREVWNHGQAADSAPFYTWKEQKDYPWSTMEERAASTEANWYDNLSTWDQ
                     PTSNNHYLNGNNYRTVNYSKKSDLNVIDFPMHWNFKNAWDAFNIAKYNDHVYADATWN
                     VTYVDSHDYAPDGAPESERFNQPQDVWAENLALMFTFRGIPAIYYGSEIEFQKGKRI"
BASE COUNT          246 a          213 c          239 g          303 t
ORIGIN      
        1 gatgcgcttt cctttttgaa attcgatctc gcttccgtaa tagatggcgg gaatgcctct
       61 aaatgtaaac atcaaggcga ggttttcggc ccacacatcc tgcggttgat tgaatctttc
      121 gctttcaggg gcgccatcag gggcgtaatc gtgtgaatcg acataagtga cattccatgt
      181 cgcatcggcg tagacatggt cgttatactt ggcgatatta aaggcatccc aggcattttt
      241 gaagttccaa tgcatcggaa aatcgatgac attaagatcg cttttcttgc tgtaattgac
      301 cgtgcggtaa ttgttgccgt tcaaatagtg attattgctt gtcggctgat cccaagtcga
      361 aaggttgtcg taccagttag cttcggttga agccgctcgt tcctccatcg tcgaccacgg
      421 atagtctttt tgttctttcc aagtataaaa aggggcggag tcggcggctt gaccatgatt
      481 ccacacttct cgcacgcgag tgcaaacttc ggcgaacatg aagaagttgt ctcccccgcg
      541 ggcttgaaag gcaggattaa aaactttatc aaagacgagg cgggaaatat gtttggcggt
      601 atcgacgcgg aagccatcga cgcccatgtc gatatagcga ttgtaggcat cgatcaagta
      661 attggtgaca taaggatttt cggtgtttaa atcgacacag tcaccggcaa tttgcccaat
      721 ctgctcgata tattgctcat agcccatgct cttttcgtga tggtagatat ggcggaagtc
      781 attcatatcc tctttcatcg catcgatgcg tgaaccgtat tggcgatcgc cgcttaatgt
      841 cgcatagtca tcaggtaata ctttataggg atcggtaatc tttagattat tgactgtatc
      901 tggttttgta taatcttttt caaacattgg aaaaaggttt tcttcgccaa aattgccagt
      961 gtgattaaag acgacatctt gaatgatttt catgcctttt g
//
