LOCUS       DAOSFC010000001        15111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_3221_length_15111_cov_4.623273, whole genome shotgun sequence.
ACCESSION   DAOSFC010000001 DAOSFC010000000
VERSION     DAOSFC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15111
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3221_length_15111_cov_4.623273"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PK791_00005"
     CDS             complement(<1..471)
                     /locus_tag="PK791_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91727.1"
                     /translation="MNKATEKLSRIFKLEAQQGFENRAVMGGMVRLTQAWESDAREVA
                     MDEKTIQFVSQKFSAYEQLSPAERKTALIEIAEQLAIQGTLNLQAFAVSEALSDFSQA
                     VSAENGRQSANINARSHLSSDKSTGIATSKSTATSSQRARVKLPVSAARPTHQAG"
     gene            complement(609..2285)
                     /locus_tag="PK791_00010"
     CDS             complement(609..2285)
                     /locus_tag="PK791_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOH91728.1"
                     /translation="MKSFSTTLPSEADRHRLLKSPLTGEGLILLFEAGLVWLTTNQEY
                     VNALNVFPVPDGDTGTNMVLTMQAGFAEADSKRSKHAGDMAKTFAHGALMGARGNSGV
                     ILSQIWRGFSRALEGHATVDAALLVKALQGARDTAYRGVVRPVEGTILTVLKDMTTAA
                     EGAIKTNSALSEVLVILVEAADVSVKNTPNLLPQLKDAGVVDAGGMGLFYIFEGMLRL
                     LQGQSLDTPATNVLSLAQFDLSHAMEQVEEGQDYEVVIDFEPNKNFLLEQYYQGLEDI
                     GVSIQVGEGEGIYRMHIHTELARRYEPIEFTETQGIIKKVYMENLQDQMSHRAEAEIK
                     CNPVHPGEIAVVAVSPGPGLSRIFASLGVAAVVEGGQTMNPSVKDLVAAFEDLPTDKI
                     IILPNNKNIILAAQNASKVSVKQVGIIPTRNTPQGFASMFRLMPDGDFDEVVESMTEA
                     ISDVHTAEITTATRTAEIEGVKVKKGEVIVLYDGKLILSEKSLDAAVEGLYEHVDMDD
                     FERITLYYGDTMNQSAANSLADKTREKYEDMEVEVHDGGQPHYQYIIAFE"
     gene            complement(2310..2645)
                     /locus_tag="PK791_00015"
     CDS             complement(2310..2645)
                     /locus_tag="PK791_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOH91729.1"
                     /translation="MGKIYILPKAIKTVVRDAALQSYGVIGLAPGNVRQSFVKMFNEE
                     GDYGISIASDERGLEITINLIVEYGLRIKSVTDSVAESVKFHVEKTMGIPVTRVNIHV
                     RGMRISDED"
     gene            2787..2972
                     /gene="rpmB"
                     /locus_tag="PK791_00020"
     CDS             2787..2972
                     /gene="rpmB"
                     /locus_tag="PK791_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOH91730.1"
                     /translation="MAKCSQCGKTTTFGHNRSHSLVATKRQFRPNLQRVTLREGNKKV
                     RRVLCTRCIKTLSKAYN"
     gene            complement(3041..3703)
                     /gene="rpe"
                     /locus_tag="PK791_00025"
     CDS             complement(3041..3703)
                     /gene="rpe"
                     /locus_tag="PK791_00025"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOH91731.1"
                     /translation="MQFRISPSILSADLSNLGEEIRACEKAGADAIHIDVMDGVYVPN
                     ITFGPNIVATCKELTNLPLDVHLMIVEPEKHIAEFVAKGADIVTVHYETCPHIHRTLQ
                     QIRQSGAKAGLSLNPGTPVEPLALLEQDLDLVLIMSVNPGYGGQKFIPVSLEKIRQAR
                     QILDRAGSKADLQVDGGIDASTVLAAYEAGARNFVAGTAVFKHPKGISGGIQALKDVL
                     NA"
     gene            complement(3708..4829)
                     /locus_tag="PK791_00030"
     CDS             complement(3708..4829)
                     /locus_tag="PK791_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOH91732.1"
                     /translation="MNNPQILNDRYQLNAKLGSGGMAHVYRAMDLMLQRNVAVKVLKE
                     DYSADLAFRERFRQEARSAANLSHPNIVTVHDFGLDQGHLFIVMEYIPGTDLKSLIKE
                     RGRLSVTESLELMIQACQGVGYAHRAGLVHCDIKPHNFLVTPDMLVKVTDFGIARALS
                     TISKDEKNDVVWGSPHYFAPEQASGLPPMPASDVYSLGVIFYELLTGKLVFDGQDSAE
                     LSRMHRTEAPIPPTVHNPDIPEQLEAIILKVLSKEPSARYRTADQLGRLLQGFYETQL
                     RGASSTLLSGPAALGVTGVGPVPVTSSPVVQAPIETPVVSTAQTYSQNVAEVVAEPVR
                     EKEPAEPVDWLTWLLGIVAFIFLIGLVPFWFYIYSMIGR"
     gene            5116..5562
                     /gene="mraZ"
                     /locus_tag="PK791_00035"
     CDS             5116..5562
                     /gene="mraZ"
                     /locus_tag="PK791_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOH91733.1"
                     /translation="MFYGRYEHNIDAKGRMTVPSIYRDQTPGNVVFITKGLDGNLMAY
                     PKPAFEIIAQTVSSKSLTDPGVRSFKREILGYTAELAYDTAGRILIPSFLRKFAGIED
                     QVVIVGVGDSFEVWSPDRLAAKEEVFADPEANQDRWVAFDISTRGN"
     gene            5562..6500
                     /gene="rsmH"
                     /locus_tag="PK791_00040"
     CDS             5562..6500
                     /gene="rsmH"
                     /locus_tag="PK791_00040"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOH91734.1"
                     /translation="MNAVHGQEAPHRPVLYQETIQYLTAKHAKRFLDCTAGAGGHSEG
                     LLRESSTDSELIALDLDSTAIALSQARLAPFGKRARVIQASYLQATEVLKAEGWDSVD
                     GILMDLGVSSMQLDQAERGFSFRYDAPLDMRFDPHQGQSAADLVNNLSESDLAQILWE
                     YGEEKFSRRIAKAIVEARPLYTTFELAEVARKAVGKTREQQDQATRTFQALRIAVNDE
                     LNVISQAVPSLIELLKSKGRIAIISFHSLEDRIIKNVFRQASLDCICPPEKLICDCGH
                     QATVKVLTKRPITASEDELHTNPRSRSAKLRVAEKL"
     gene            6530..7018
                     /locus_tag="PK791_00045"
     CDS             6530..7018
                     /locus_tag="PK791_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91735.1"
                     /translation="MSTTTTPSQAYKQSPWRRQMQVIGAFMLVLVIVATIAGLYLSIS
                     GRAAAAGRRIQELEMQATTLNLEINNLRTRLAQVSSSHALNERISDLNMRILDPHTTI
                     YLEVPGYSPDIRKGSGNQPAPVTVPKAKLLPEYTSSLWDWLREKIWTVSPQPSIIDEV
                     TP"
     gene            7015..8823
                     /locus_tag="PK791_00050"
     CDS             7015..8823
                     /locus_tag="PK791_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOH91736.1"
                     /translation="MNAQNGHGRILTICILATLIAVAICVQFIRIQNMDVAQQILDDS
                     KAYQGVTRLVYPNRGNIYDAKGRLLAGNETTYEVGLDLNAILNGQEETIASAVSEILG
                     MDYDKVLAYAMIEPGNGNPFYIQLDGFVSPDKILEFEALIEEYKNMELGRNQIRPRLN
                     GLMWTAHNKRSYPEGTTGSNILGFYKYLDRTQGMGYFGVEETYNTLLAGTPQMVYTAF
                     DPQKLSMLDEIPAGADIELTFNREIQAMAEQELDEAIEWSGAKGGTILIYNPKNGEII
                     AMANTPRLDPNEYWNYGELFPNPEPFNPTISKTYEPGSVFKAITVAIALDTGAIEMDT
                     VYSDDGAYEIGGVEIRNWDYGAYGDVDMTDCMRWSLNTCLAWMAEEIGPDNFYRYLKA
                     FGFDRNTGIDLGGETHWPLKLPGDSNWYEIDLATNAFGQGISVTPIQMAMTVGALANE
                     GKMYVPHVVKAVIIDGRRYEVEPVLAGTPVSAETARQVTAMLTETLMDESYENAYIDG
                     YTFAGKTGTGQIPTEYGYTNPLTNASFVGWGPSEDPQVVIYVWMQEPTRDIWGSQIAA
                     PIFSKVVEKLVVLMDIPPDSIRLMNQPDRQVMEAGE"
     gene            8826..10247
                     /gene="murF"
                     /locus_tag="PK791_00055"
     CDS             8826..10247
                     /gene="murF"
                     /locus_tag="PK791_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOH91737.1"
                     /translation="MSVLSISHILKALKSPIQTVEADIPVYGGCIDSRRCEDGSLFIA
                     IKGEVTDGHFYVDNAFNNGAYMAIIDHAIESDYPVIDIADPNAIIPTEAPYSLLVPDS
                     ILALQTIATYWREQFDIPVVGITGSVGKSSSKDLIASVLSHRMRTLKNPGNYNNEIGL
                     PLTLLSLTHEHEIAVLEMGFYVAGEIKLLCDIAHPNIGVLTNIGTVHAERAGSIEAIA
                     EGKAELLEELPEAPKGLAILNYDDDFVREMASKTHARVFYYGLDPRADLWADKIESRG
                     LNGIRCQLHHQGESYYLTAPLIGRHSVFTILRACAVALNLGISWDWIFHAFKSSREQV
                     RVITVKTPSGALIIDDTYNASPDSTLAALNLLGDIDGRKVAVLGDMLELGQYEREGHR
                     RVGLRASEIADEIVLIGTRSQITRDAVLETEFKPENLRWFENTDEAIAYLKPRLSQGD
                     IVLVKSSHSMHLERIVSALEEVH"
     gene            10247..11245
                     /gene="mraY"
                     /locus_tag="PK791_00060"
     CDS             10247..11245
                     /gene="mraY"
                     /locus_tag="PK791_00060"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOH91738.1"
                     /translation="MENTTIAIAVSGIAFLLTVIWGDPFIRLLKHWKVGKIIRVDDLP
                     ESHREKMNVPTMGGLMFILPVTLLTVILNAVTLFGSRVFGMSILVPLACMWGFAIIGA
                     IDDWEGIRGPRRGIGMSSKVKLILQIIVALVIAYMLRYQLRVPEMIWPVTGGYITIGW
                     PYVIIAAFIIVAMANAVNFTDGIDGLAGMISLTAFGIYGIVAMTQGQIYLARFCFCIV
                     GALAGFLWFNVKPAQLFMGDTGSQALGATLAVVALMTGQWLWLPLIAIIPTAEMLSVV
                     IQVIYFKATGGRRVFKKTPIHHHFEMIGWEESQIVMRFWLIELIGAMIGLSMTFIG"
     gene            11249..12637
                     /gene="murD"
                     /locus_tag="PK791_00065"
     CDS             11249..12637
                     /gene="murD"
                     /locus_tag="PK791_00065"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOH91739.1"
                     /translation="MKKYDSKNILIIGAARQGLAASRYLDKHGARVTLTDARPEAELT
                     DAKQSLADTQVKTHFGAHPLELLDGVDLVCVSGGVPLDIPIIREARKRRIKLSNDSQI
                     FLENCPCTVIGITGSAGKTTTTTLLGKMAEQFFAMKTHKNRAWTGGNIGYPLINNVDE
                     MHEDDLAIMELSSFQLELMTRSPHVAAVLNITPNHLDRHGTLGAYTAAKARILKFQKQ
                     ADLAILNRDDKGSWDLRDELHGDLISFGYKQPGRNEHGTYVENGNIMLQIARERMKML
                     PIEWIPLRGEHNLQNVLAACAIAAGATIALPAIQTAIEEFEGVSHRLQLVATRNDVKW
                     YDDSIATTPERSLAALKSFDEPIILLLGGRDKNLPWEDLLAFASQRAKHIILFGEASP
                     MIAEILGRGKKPANYLSLSQAGTLQNAVKIAAEKAEAGDIVLLSPGGTSYDAFIDFEE
                     RGNCYAKWISEL"
     gene            12634..13857
                     /locus_tag="PK791_00070"
     CDS             12634..13857
                     /locus_tag="PK791_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOH91740.1"
                     /translation="MSTQVQAQAKPVGVKRLGIDVPLLIIVACLVVFGLLMVYSSSWD
                     ASLRAGKAPTHIFNRQLIWVGIGLVICLIASLTDYHFYSKVVVIMMIGVLILLIAVLI
                     VSRVKSDATRTLFDGSGQPTELAKMATILYLAVWLNNRRETLGSFKDGLIPLVAIVGL
                     VAALIIFQPDISAAATIGILGIIMFFIGGGDLRQIMLSLAGGLGLGLLAVNILPTGRA
                     RISQYLSGIQNPVKGSYHIRRTFEAVIKGRFFGVGLGNADTKFTGLPLPHTDSIFAVI
                     AEETGVFGSFFVIILFVLLLWRGYVIAKNAPDNLGKLLSFGLIGWIVLEALMNIAVIV
                     GLFPMAGNALPFISAGGSSMVTTLFAIGIVMNVSRQSAAKINQERSSGSAAVDLRGRD
                     WRRSVSRPNRYRDTE"
     gene            13820..14854
                     /gene="murG"
                     /locus_tag="PK791_00075"
     CDS             13820..14854
                     /gene="murG"
                     /locus_tag="PK791_00075"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOH91741.1"
                     /translation="MYPALTVTEILNKAGHDILWVGTADGIEANLVQRAGIPFEGIRA
                     AGVHGVSIKKLPGNLLKLWKGYRDSKAILKRFKPDVLLFTGGFVAVPMALAGRHVPSL
                     LYVPDIEPGLALKTLGRFAKKIAITTEDTKMFYKSSDKLVVTGYPLRDELKQWTKPEG
                     LEYFHFSPDLPTILFMGGSTGARSINKAVISIADKLVEKYQVIHLVGHLDWEKVSKAT
                     ESLGARYQAFPYLHEVGAAMAAADLVVSRAGASALGEYPYFALPAILVPYPYAWQYQK
                     VNADYLVDRDAAILLEDSRLMTELHETIENLMQNPGKLARMSNAMRSLTHPNAAENIA
                     ELLYEMAGAA"
     gene            14851..>15111
                     /locus_tag="PK791_00080"
     CDS             14851..>15111
                     /locus_tag="PK791_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOH91742.1"
                     /translation="MRTVHLIGIGGTGMSAIAIFLLEKGYKVTGSDKNRSSYFDLVRA
                     KGADARVGSHPDLATQADVIVRSSAIRDEDPEVIAGKAAGIPV"
BASE COUNT         3779 a         4080 c         3528 g         3724 t
ORIGIN      
        1 gcctgcctga tgcgtcggtc gtgcggcact gactggcaac tttacccgcg cgcgttggct
       61 acttgtggct gttgatttgc ttgtagctat gccagttgat ttatcgcttg acaagtggct
      121 acgcgcatta atgtttgcgg attgacgccc attttccgca cttacagctt gagaaaaatc
      181 ggataaagcc tcagacaccg cgaaagcctg cagattcagc gttccctgta ttgccagctg
      241 ttcagcaatt tcgataagcg cggttttgcg ctccgcggga cttagttgtt cataagcgct
      301 gaacttttga ctgacaaatt gaatcgtctt ttcgtccatg gcgacttcgc gggcatccga
      361 ttcccatgcc tgggtcagcc tcaccatgcc acccatcacc gcgcgattct caaacccttg
      421 ctgggcttca agcttgaata ttctggataa tttttccgtg gctttattca tcggtctttc
      481 tctgtctaat gataccaaaa tccacttgat gccttcaaga gttagaagaa cgtcattgta
      541 aaaaaaagta acccgcgctt gattaatcac gggttacttt tagaaaatgc taattcgcga
      601 acagctacct actcaaacgc gatgatgtac tggtaatgcg gttggccacc atcgtggact
      661 tcaacttcca tgtcttcgta tttctcgcgg gttttgtcag ccaggctatt ggctgcggac
      721 tggttcatgg tgtcgccata atacaaggta atccgttcaa aatcgtccat atcaacgtgc
      781 tcatacaacc cctcaaccgc tgcgtcaagt gacttttcac tcaaaatcag cttgccgtcg
      841 taaagcacaa tcacttcacc ttttttaact ttcacgcctt caatttcagc tgtgcgggtg
      901 gcggtagtaa tctcagcggt atgcacgtcc gaaatcgcct cggtcatcga ctcaaccact
      961 tcatcaaaat cgccatcggg catcaggcgg aacatggacg caaatccctg cggtgtgttt
     1021 ctggtgggaa taatgcccac ctgtttgaca ctcacttttg aagcgttttg agcagctaaa
     1081 atgatgtttt tgttgttcgg cagaatgata atcttatccg taggcaaatc ttcaaacgcg
     1141 gcaaccaggt ctttcacgct ggggttcatg gtttgcccac cttctaccac tgctgccacg
     1201 cccaggcttg cgaatatgcg cgacaatccc ggccctggcg aaaccgccac aaccgcaatt
     1261 tcacccggat gaaccggatt acatttaatt tcagcttcag cgcgatggct catttggtcc
     1321 tgcagatttt ccatgtagac ttttttgatg ataccttgcg tttctgtaaa ttcaatcggc
     1381 tcatagcgtc gtgccagttc agtgtggatg tgcatgcgat aaatgccttc gccttcacca
     1441 acctgaatac tcacgccgat atcttccagt ccctgatagt actgctcaag caaaaagttt
     1501 ttattaggct caaagtcaat cacgacctcg tagtcttgcc cttcttcaac ttgctccatg
     1561 gcatgcgata aatcgaactg cgccaaggaa agcacattcg ttgcaggcgt atccagcgac
     1621 tgaccctgaa gcaagcgcaa catgccttca aagatgtaga acaggcccat accaccggca
     1681 tcaaccacac cagcgtcttt gagttggggt aataagtttg gcgtattctt tacactgaca
     1741 tcagcggctt cgaccaaaat gaccaaaact tcactcaagg cgctgttggt tttaatggca
     1801 ccctcagcag ctgtggtcat atcttttagg acagtcaaaa tggtgccttc aaccgggcga
     1861 acaacgcccc gataagcagt gtcgcgcgca ccctgcaggg cttttaccag caaagcggca
     1921 tccaccgtgg catgtccttc cagcgcgcgt gaaaaaccac gccaaatttg tgacaaaata
     1981 acacctgagt tacctcgcgc gcccatcaac gcaccgtgcg caaaagtttt ggccatatca
     2041 ccggcatgtt tggaccgctt ggaatctgct tccgcaaagc ctgcttgcat ggtaagcacc
     2101 atatttgtgc cggtatcgcc atcgggtacc gggaaaacat ttagcgcgtt cacatattcc
     2161 tgattggtcg ttagccagac taatccagct tcgaaaagta agattagtcc ctctcctgtc
     2221 agtggtgatt tcaatagccg atggcggtct gcctcgcttg gtaaggtggt tgaaaatgac
     2281 ttcataagac tcctacgaac aaataaaatt taatcttcat cactaatacg cataccacga
     2341 acgtgaatat taacgcgcgt gacaggtatt cccattgttt tttccacatg gaacttcaca
     2401 ctttcagcca ccgaatcggt cacgctttta atgcgaagtc catactcaac gataaggttt
     2461 atggtaattt ccagaccacg ctcatcagaa gctatggaaa taccataatc accttcttca
     2521 ttaaacattt ttacaaatga ttgcctgaca ttgcccggcg caaggcctat aacgccatag
     2581 gattgcaacg cggcatcacg tactactgtt tttatagcct ttggcaaaat ataaattttg
     2641 cccattgggt tctcccgact tgtcatcttt cgtgcctcac ttccatgtaa tacttgattc
     2701 tttttattct atctgttatc attattattt gcgtgcggcg tttaccacct tcacgctaga
     2761 aattataacg caagaaaggt aataaaatgg caaagtgctc tcaatgtggt aaaacaacca
     2821 ctttcggtca taatcgcagt cactcactcg ttgcgacaaa acgtcagttt cgtcctaatt
     2881 tacaacgggt cacgctaagg gaaggcaaca agaaggttcg ccgcgtgctt tgtacccgct
     2941 gcatcaaaac cttgtccaaa gcgtacaact aggtttataa cacagtaaag cgtaaaaagt
     3001 cgcgtttgcg gcttttttgc ttttaagccc cactatcctt ttacgcgttt aaaacgtctt
     3061 ttaaagcttg aattccaccc gaaatacctt tgggatgttt gaaaacagca gtgcccgcca
     3121 caaaattgcg cgcgccggct tcatatgctg caaggaccgt cgatgcatca atgccaccgt
     3181 ccacctgcaa atccgccttg gaaccagccc ggtcaagaat ttgcctggcc tggcgtattt
     3241 tttcaagtga aacaggaata aacttttgcc cgccataacc cgggtttacc gacataatca
     3301 agaccaaatc cagatcctgc tccaagagtg ccaaaggctc aacaggcgtt ccaggattta
     3361 agcttaagcc cgccttcgcg cctgattgcc tgatttgctg caaggtacgg tgaatatgcg
     3421 gacaggtttc ataatgcaca gtaacgatat ccgcgccttt ggcgacgaat tccgcgatgt
     3481 gtttttccgg ctctacaatc atcagatgca cgtccaaagg taaattcgtc aattctttac
     3541 aagtcgcgac aatatttggc ccaaaagtga tgttcggcac atagacgcca tccatcacgt
     3601 caatgtggat agcatccgcc ccggcttttt cacaggctcg gatttcttcg cccaaattgc
     3661 ttaaatcagc agaaagtatt gatggtgaaa ttcgaaattg cataagtcta tcgccctatc
     3721 atgctataaa tgtaaaacca aaacggcacc agccctatca aaaagataaa cgccacaata
     3781 cccaaaagcc aggtcagcca gtccactggc tcagccggtt ctttctccct gactggctca
     3841 gcaactactt cagccacatt ctgtgaataa gtttgtgcgg tacttacgac aggtgtttca
     3901 atcggcgcct gaacaacagg actggatgtt accggaacag gcccaacgcc agtaacacca
     3961 agcgctgccg gtcccgaaag caaggtactg gaagcgccac gcaactgcgt ttcgtaaaaa
     4021 ccttgcaaaa gccgtcccag ctggtctgct gtgcggtagc gcgcgctggg ttcttttgaa
     4081 agcaccttca ggataatagc ttcaagttgt tcggggatat ccgggttatg gacggtcggc
     4141 gggatgggtg cctctgtgcg atgcatacgc gagagttcgg cagagtcctg cccgtcaaac
     4201 accagcttgc ccgtcagcag ttcgtaaaaa ataacgccca aagaataaac gtccgaagca
     4261 ggcatcggtg gcagaccact ggcttgctcc ggcgcgaaat aatgcggcga cccccaaacc
     4321 acgtcgtttt tctcgtcttt tgaaatcgtc gaaagtgccc gcgcgatgcc aaaatcggtc
     4381 actttgacca gcatatccgg cgtcaccaaa aagttatgcg gtttgatgtc acaatgcacc
     4441 aaacccgccc ggtgcgcgta tcccacgccc tgacaagcct ggatcatcag ctccagggat
     4501 tcggtcacac tcagacgccc acgctctttt atcagcgatt ttaagtccgt gccggggata
     4561 tattccataa cgatgaacaa gtgcccctgg tccaagccaa aatcatgcac ggtgacgatg
     4621 ttggggtgcg aaaggttggc agccgaacgt gcttcctggc ggaaacgctc cctgaaagcc
     4681 aggtcagctg agtagtcttc tttcaggact ttcaccgcca cattgcgctg aagcatgaga
     4741 tccatcgccc ggtaaacgtg cgccatacca ccactgccaa gtttggcatt cagctgatat
     4801 cggtcgttca aaatctgcgg attgttcatc accccgatta taacgcatta tctcccagaa
     4861 ctttgctgga attgtagctt tggtgtcacg caatcccatc agaaaaatga attcccaaaa
     4921 ccagaacaaa caaatctcga gagaaacagg aaaatcttgc ccattttggc aaattaatcc
     4981 cttaaaaccc ttgctttttt gtggcttaag gtctataatg tgggaaaatt tgtcataaag
     5041 tgtcacaaag tgtcacgcat gtagtgaaag cgctggaaac ccggcgcttc ggccattaaa
     5101 aagggattag aacaaatgtt ttacggtaga tacgaacaca atattgatgc taaaggtcgc
     5161 atgaccgtgc catcaattta ccgggaccaa acccccggca atgttgtgtt catcaccaaa
     5221 ggcctggacg gaaacctgat ggcttatcct aaacccgcct ttgaaattat agctcaaacc
     5281 gtaagcagca aatcgcttac cgatcctggg gtgcgttcct tcaaacgcga aatccttggc
     5341 tacactgctg aactggctta tgacacggct ggccgtattc tcattccgtc tttcttgcgg
     5401 aagtttgccg gaattgagga tcaagttgtc atcgttggcg ttggagatag ttttgaggtt
     5461 tggtctcccg acagattggc tgcaaaagaa gaagtgtttg cagaccccga agccaaccaa
     5521 gaccgctggg tcgcctttga catatcgact agaggaaatt aatgaacgcg gtgcatgggc
     5581 aagaagctcc gcaccgcccg gtactttacc aagagacaat tcaataccta actgcgaagc
     5641 acgctaaacg ctttttggac tgcactgccg gtgctggtgg tcattctgaa ggtcttttgc
     5701 gcgaaagctc aaccgacagc gaactcatcg ctttggattt agattcaacc gctatcgcgc
     5761 taagccaggc aagactcgcc cctttcggca aacgtgccag ggtcattcaa gcctcatacc
     5821 ttcaggcaac agaagtgctc aaagcagaag gctgggacag cgtggacggc atcctgatgg
     5881 atttgggtgt ttcttccatg cagctggatc aggctgaacg tggtttctcg tttcgctacg
     5941 acgccccact ggacatgcgc tttgacccac accaggggca atcagcagct gatttggtga
     6001 acaatcttag cgaaagcgac ttagcccaaa tcctttggga gtatggcgaa gagaagtttt
     6061 ccagacgcat cgctaaagcg attgtcgaag ccagaccgct ttacaccact ttcgaactcg
     6121 ccgaagtcgc cagaaaagca gttgggaaaa cccgcgaaca gcaagaccag gccacacgca
     6181 cctttcaggc cttgcgcatc gcggtgaacg acgaactcaa tgttattagc caggccgttc
     6241 caagcctgat tgaactctta aaatcaaaag ggcgtatcgc gatcatcagt tttcattcgc
     6301 ttgaagaccg cattatcaaa aatgtctttc gtcaagcgag tctcgattgc atctgcccgc
     6361 cagaaaagtt gatttgcgac tgtggacacc aagccaccgt caaagtcctc accaagcggc
     6421 ccatcaccgc cagcgaggac gaactgcaca ccaacccacg atcccgcagt gccaaactgc
     6481 gcgtcgcgga gaaactataa aaggcatagt atttgcaata ttaaaaggta tgagtacgac
     6541 aacaacacca tcacaagcct ataaacaaag cccttggcgt cgccagatgc aagtcatcgg
     6601 cgcgttcatg ctggtcttgg tgattgttgc caccatcgct gggctctacc tttccatcag
     6661 cggacgtgca gcggcagctg gccgacgcat tcaggaactt gaaatgcaag ccaccacgct
     6721 gaatctcgaa ataaacaacc tgcgcacgcg cctggcacaa gtttcatcgt cgcacgcgct
     6781 taacgaacgc attagtgatt tgaacatgcg aatccttgac ccccatacca ccatttacct
     6841 ggaagtgccc ggttacagcc ctgacattcg caaaggcagt ggaaatcagc cagcgccagt
     6901 aactgtgcca aaagccaaac tcttaccgga gtacaccagt tcactttggg actggttgcg
     6961 cgaaaaaatc tggacggttt ctccgcagcc ttcaattatc gacgaggtca caccatgaac
     7021 gcgcaaaatg gtcacggacg catcttaacg atttgcattt tagcgaccct catcgcggtc
     7081 gcgatttgcg tgcagtttat tcgcattcaa aacatggatg tcgctcagca aatccttgat
     7141 gacagcaaag cctaccaggg tgttacccgc ctggtttacc ccaaccgtgg caatatttat
     7201 gacgccaaag gacgcttgtt agccggcaac gagaccactt acgaagtcgg acttgacctg
     7261 aacgccatcc tgaacggaca ggaagaaacc attgcctctg ctgtttccga aatcctcggc
     7321 atggattacg acaaagtcct ggcatacgcc atgattgaac cgggtaatgg caatccgttc
     7381 tacattcagc tggacggctt tgtcagccca gataaaatcc tggaatttga agctttaatc
     7441 gaagaataca aaaacatgga actgggacgt aatcaaatcc gccccagact caacgggctc
     7501 atgtggactg cccacaacaa acgcagttat cccgaaggca ccaccggctc gaatatactc
     7561 ggcttctata aatatctcga ccgcacccag gggatgggtt acttcggcgt tgaagaaact
     7621 tataacaccc tgttagccgg cacaccccaa atggtttaca ccgcgtttga cccgcaaaag
     7681 ctttccatgc ttgatgaaat cccggccggt gccgacattg aactcacctt caaccgcgaa
     7741 attcaggcca tggcggaaca agaacttgac gaagccatcg agtggtctgg cgccaagggc
     7801 ggcacaattc tcatctacaa ccccaaaaat ggcgaaatta tcgccatggc caacaccccg
     7861 cgccttgacc cgaacgagta ctggaattac ggtgaattat tcccaaatcc cgaacctttc
     7921 aacccaacca tcagtaaaac ttatgaaccc ggttcggttt tcaaagccat cactgttgcc
     7981 atcgccctgg ataccggtgc tattgaaatg gacaccgttt attcagatga tggcgcttac
     8041 gaaatcggtg gcgttgaaat tcgcaactgg gactacggtg cttatggcga cgtagacatg
     8101 accgattgca tgcgctggtc gctcaacact tgcctggcat ggatggctga agaaatcggt
     8161 cctgataact tctaccgcta cctgaaagcc ttcggtttcg accgcaatac cggcatcgac
     8221 ctgggtggcg aaacccactg gcccctgaaa ctgcccggtg acagcaactg gtacgaaatc
     8281 gacctggcta caaacgcctt cggacagggt atttccgtta cgcctatcca aatggcgatg
     8341 accgttggcg cgctggccaa cgaaggtaaa atgtacgttc cccacgtggt aaaagcggtc
     8401 atcatcgacg gtagacgtta tgaagttgag ccggttttgg caggcacgcc cgtgagtgcc
     8461 gagaccgcgc gtcaggtgac cgccatgctc accgaaaccc tgatggacga atcgtacgaa
     8521 aacgcctata tcgatggtta caccttcgcc ggcaaaaccg gtaccggtca aattcctacc
     8581 gaatacggtt acaccaaccc gcttaccaac gcgtcgtttg ttggctgggg tccttcagaa
     8641 gacccgcaag ttgttattta cgtgtggatg caggaaccca cgcgcgatat ttggggcagt
     8701 caaattgccg ctccgatttt ctcaaaagtg gttgaaaagc tggttgtctt gatggacatc
     8761 ccccctgaca gcatccgtct tatgaaccag ccagaccggc aggtcatgga agcaggagag
     8821 taaaaatgag tgtattaagt atttcccaca tcttaaaggc actcaaatcg cccattcaga
     8881 ccgttgaagc ggatattccc gtttacggtg gctgtattga ttcacgtcgc tgcgaagatg
     8941 gctcgctgtt tatcgccatt aagggtgaag ttactgacgg acacttttac gttgataacg
     9001 cattcaataa tggcgcttat atggcgatta ttgaccacgc catcgaatcg gattacccag
     9061 tgattgacat cgctgacccg aatgcgatta tccccaccga agcaccttat agtttgctgg
     9121 tgccagattc aattcttgcc ttacaaacca tcgccactta ctggcgggaa cagttcgata
     9181 tccccgtcgt cggcatcacc ggcagtgtgg gcaaatcttc cagcaaagat ttgattgcca
     9241 gcgtgctctc gcatcgcatg cgcacgctca aaaaccctgg caactacaac aatgaaatcg
     9301 gcttgccctt aaccctgcta agcctgaccc atgaacatga aatcgccgtg ctcgagatgg
     9361 gcttttatgt cgcgggcgaa atcaaactcc tttgcgatat cgcgcatcct aatatcggtg
     9421 tactcacgaa tattggcacc gtgcatgccg aacgcgctgg ctcaattgaa gccatcgcgg
     9481 aaggaaaagc tgaattgctc gaagaactgc ccgaagcccc aaagggcctg gcaattttga
     9541 attatgacga tgattttgtc cgcgaaatgg cttcaaaaac ccatgcccgc gttttctact
     9601 acggacttga tccccgcgct gacctttggg ctgacaaaat tgaaagccgt gggctgaacg
     9661 gcatccgctg ccaactgcac catcagggcg aatcttacta tctcaccgct ccactcatcg
     9721 gacgccattc cgtctttacc atcttgcgcg cctgcgctgt ggcgctcaac ctgggcatca
     9781 gctgggactg gatttttcac gccttcaaaa gcagtcgaga acaggtacgc gtcattaccg
     9841 tcaaaacgcc ttccggcgcg ctcatcatcg acgacaccta caacgcctca cctgattcaa
     9901 cccttgccgc gctcaacttg cttggcgaca tcgacggacg caaggttgcc gtactgggcg
     9961 atatgcttga acttggccag tacgaacgcg aaggacaccg ccgcgttggc ttgcgcgcgt
    10021 cagaaatcgc tgatgaaatc gttctgattg gcacacgcag tcaaatcacg cgcgatgctg
    10081 tccttgaaac cgaatttaag cctgaaaacc tgcgttggtt cgaaaacacc gatgaagcca
    10141 tcgcctactt gaaaccacgc ttaagccagg gcgatattgt gctcgtcaaa agttcacaca
    10201 gtatgcatct tgagagaatc gtctcagctt tggaggaggt ccattaatgg aaaataccac
    10261 aatcgcgatt gcagtcagtg gcattgcctt cttgttaaca gttatttggg gcgatccttt
    10321 cattcgcctg ctcaaacact ggaaagtggg caaaatcata cgtgttgacg acctgcctga
    10381 atcgcaccgt gaaaaaatga acgtccccac catgggtggc ttgatgttta ttctgccggt
    10441 taccttgtta accgtcattt taaacgcggt aacgctattt ggttcacgcg tatttggtat
    10501 gtcgatctta gtccccctgg cctgtatgtg gggtttcgcg attattggcg cgattgacga
    10561 ctgggaaggc atccgtggtc cccgacgcgg tattggtatg tccagcaaag tgaagctgat
    10621 cttgcaaatt attgtcgcgc tggttatcgc ctatatgctg cgctatcaat tgcgtgtccc
    10681 tgaaatgatt tggcccgtca ccggtggcta tattaccatc ggatggcctt atgtcattat
    10741 cgctgctttt attattgttg ccatggcaaa tgccgtcaac tttaccgatg gcatcgacgg
    10801 tttagccggc atgatttcac tgaccgcgtt tggcatttat ggcatcgtcg ccatgactca
    10861 ggggcaaatc tacctggcac gattttgttt ttgtatcgtt ggcgctttgg caggcttttt
    10921 gtggttcaac gtcaagcccg cgcaactctt tatgggcgat accggctccc aggcattggg
    10981 cgccacgctg gcggtcgtcg ctttgatgac cggccagtgg ctctggctcc ccctaatcgc
    11041 catcattcct actgcggaga tgctctcggt cgtcattcag gtgatttact tcaaagccac
    11101 aggcggacgc cgggttttca agaaaacacc tattcaccat cactttgaga tgattggctg
    11161 ggaagaaagc caaatcgtaa tgcgattttg gctcatagaa ctgattggcg caatgattgg
    11221 cctaagcatg acatttattg gataaacgat gaaaaaatac gactcaaaaa acattctgat
    11281 tattggcgcg gcccgacaag gactggcagc atcgcgttat ctcgataaac atggtgccag
    11341 agtaacttta accgacgcgc gccctgaagc agaactgact gacgccaaac aaagcctggc
    11401 agacacccag gtgaaaaccc actttggcgc gcatccgctc gaacttcttg atggcgttga
    11461 ccttgtctgc gtttccggtg gcgtgccttt ggatattccc atcatccgag aagcccgcaa
    11521 gcgacgcatc aagctaagca atgattcgca gatatttctt gaaaactgcc cctgcacggt
    11581 catcggcatc accggttccg ctggaaaaac aaccacgact accttgcttg gcaaaatggc
    11641 ggaacagttc ttcgccatga agacccataa aaaccgcgcc tggaccggtg gcaacatcgg
    11701 ctacccgctc atcaacaatg ttgacgagat gcacgaagac gacctggcca ttatggaact
    11761 ttccagcttc cagctcgaac tgatgacccg ctccccccac gtcgctgccg tgctaaacat
    11821 cacccccaac caccttgacc gacatggcac cctgggcgca tataccgccg ccaaagcgcg
    11881 catcctgaaa tttcaaaagc aagctgacct tgccattctc aaccgtgatg acaaaggttc
    11941 ctgggactta cgcgacgaac tccacggcga cctaatcagc tttggttaca aacaacctgg
    12001 cagaaatgaa catggcacct acgttgaaaa cggaaacatc atgcttcaga ttgcccgcga
    12061 acgcatgaaa atgttgccca tcgagtggat tcctttacgc ggtgaacaca acctgcaaaa
    12121 tgttttggca gcctgtgcca ttgccgctgg cgcgaccatc gccctgccgg ctattcagac
    12181 cgccatcgaa gaatttgaag gcgtttcgca ccggttgcaa ctcgtcgcga cgcgcaacga
    12241 cgtaaagtgg tatgacgact ccatcgccac cacacccgaa cgctcccttg ccgcgttgaa
    12301 atcttttgac gaacccatca ttctgctttt gggtggtcgc gataaaaacc tgccctggga
    12361 agacttgctc gcctttgcca gtcaaagagc caaacacatc attttgtttg gcgaagccag
    12421 cccgatgatt gcagaaatcc ttggtcgtgg caaaaaacca gccaactatt tgtcgctcag
    12481 ccaggctgga acccttcaaa atgcggtcaa aatcgctgcg gaaaaggctg aagccggcga
    12541 catcgttctg ctctctcccg gtggcaccag ttatgacgct tttattgact ttgaagaacg
    12601 tggcaactgc tacgcgaaat ggataagtga gttatgagta cccaagttca agcccaggca
    12661 aaaccagtcg gcgtaaaacg ccttggcatc gatgtgccct tgttaatcat cgttgcctgt
    12721 ttggtcgttt ttggcttgct gatggtctat tcttcaagct gggacgcctc attacgtgct
    12781 ggaaaagcac caacacatat tttcaaccgc cagcttattt gggttggaat aggtctagtg
    12841 atttgtttaa tagcgagttt gactgattat cacttttact caaaagtagt ggtcattatg
    12901 atgataggtg ttctcattct gctcattgca gttttgattg ttagccgcgt gaaatctgat
    12961 gctacccgaa cattgtttga tggctcaggg cagccaacgg aattggcaaa aatggctacc
    13021 attttgtatc ttgcggtttg gctcaacaat cgacgcgaaa ccttgggtag cttcaaagat
    13081 ggtctcattc cactcgtcgc tattgtaggc ttggtcgccg ccttgattat ttttcaacct
    13141 gatatcagcg ctgctgctac tattggcatc ttgggcatta tcatgttctt cattggcggt
    13201 ggagatttgc gtcaaattat gttgtctttg gctggtggtc ttggtcttgg tcttttggcg
    13261 gtcaacattt tacccacggg acgagcgcga atcagtcaat atctcagtgg tattcaaaac
    13321 cctgttaaag gctcctatca catccgacgc accttcgaag ccgtcatcaa aggacgcttc
    13381 ttcggggttg gacttggcaa tgccgatacc aaattcaccg gcctgcccct gccccatacc
    13441 gacagtatct ttgccgttat cgctgaagaa accggcgttt tcggctcatt tttcgtcatt
    13501 atcttgtttg ttcttttgct ctggcgtggt tacgttatcg ctaaaaacgc gccggacaat
    13561 ttaggcaaac ttttatcatt cggactaatc ggatggattg ttttggaagc actgatgaat
    13621 atcgcggtta ttgttgggct tttccccatg gcaggaaacg ctttgccctt catcagcgcg
    13681 ggtggctcaa gcatggtcac caccctattt gccatcggca ttgtgatgaa cgtttcgcgt
    13741 cagagtgccg caaaaataaa tcaggaaagg agttcaggca gtgcggctgt tgatttgcgc
    13801 gggcgggact ggcggcggag tgtatcccgc cctaaccgtt accgagatac tgaataaagc
    13861 tggtcacgac attctctggg ttggcacagc cgacggcatc gaagctaatt tggtgcaacg
    13921 cgctggcatt ccctttgaag gcattcgcgc cgctggcgtg catggtgtgt ccattaaaaa
    13981 actgcctgga aacctactaa aactatggaa aggctaccga gactcaaaag cgatattgaa
    14041 acgcttcaaa cccgacgttt tgctcttcac cggcgggttc gtggcagtgc ctatggcatt
    14101 agccggacgt cacgtgccgt ctttgcttta tgtgcccgac atcgaacccg gtttggcgct
    14161 aaaaacgctc ggtcgctttg ccaaaaaaat cgcaattacc acggaggata ccaaaatgtt
    14221 ctataagtct tccgataaac tcgttgttac cggctatccc ttgcgcgatg aactcaagca
    14281 atggaccaag cccgaaggtt tggaatactt ccactttagt cccgatttgc ccaccatttt
    14341 gttcatgggt ggcagcactg gcgcacgttc gattaataaa gccgtcatca gcattgccga
    14401 taaactggtc gaaaagtacc aggtcatcca tcttgttggg catctcgact gggaaaaagt
    14461 cagcaaggcc accgaatcgc ttggcgcgcg ctatcaggcc ttcccctatc tgcacgaagt
    14521 cggtgccgcg atggcagccg ctgatttggt cgtctcacgc gctggcgcga gtgccttggg
    14581 tgagtatccc tacttcgctc tgccggccat tttagtacct tacccctacg cctggcagta
    14641 ccaaaaagtc aacgcggact atctggtaga ccgcgatgct gccattctgc tggaagattc
    14701 acgcctgatg actgaactcc atgaaactat tgaaaacctg atgcaaaacc caggcaaact
    14761 cgcccgcatg tccaacgcca tgcgctcgct aacccatccc aacgcggccg agaacatcgc
    14821 cgaactactc tacgaaatgg caggcgcagc atgagaacag tgcatctgat cggcatcggt
    14881 ggaacaggca tgagcgcgat tgccatcttt ttacttgaaa aaggctacaa agtgaccggc
    14941 agcgacaaaa accgctcgtc atactttgac cttgtccgcg ccaaaggcgc tgacgcgcgc
    15001 gttggcagcc atcccgacct tgccacacag gccgacgtca tcgtgcgttc ttccgccatc
    15061 cgcgatgaag accccgaagt tatcgccggc aaagccgcag gcattcccgt g
//
LOCUS       DAOSFC010000002        15065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_3238_length_15065_cov_6.200000, whole genome shotgun sequence.
ACCESSION   DAOSFC010000002 DAOSFC010000000
VERSION     DAOSFC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15065
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3238_length_15065_cov_6.200000"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PK791_00085"
     CDS             <1..438
                     /locus_tag="PK791_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOH91743.1"
                     /translation="PTNFGNPRRIANGINFNRLLIIVAVLTRRVGLNLGNQDVITNVV
                     SGLRLEEPAADLAVATALASSMRDLPTRADTVLIGEVGLSGELRMVGQMPTRLREAAK
                     LGFKRAIVPRRARKGEPWPDTIEILEARSLRDALKLAFVEEEK"
     gene            614..1132
                     /locus_tag="PK791_00090"
     CDS             614..1132
                     /locus_tag="PK791_00090"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HOH91744.1"
                     /translation="MKKNILDYIPIIRRIRRNHALEHATMHVLSERYPGLRMMGISNP
                     KGFLMVADLPTELMTEATLEAKNRLKAGETDLAVHPNCGSNIAVPTLVASMVAGSVLA
                     LSTVNEKKPKWYHYLIAAASAVPAYFLARPLGPITQKYITTSGEIEDTQVKGVLTQKV
                     LNGSVHKVDTEA"
     gene            1136..2158
                     /gene="holA"
                     /locus_tag="PK791_00095"
     CDS             1136..2158
                     /gene="holA"
                     /locus_tag="PK791_00095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOH91745.1"
                     /translation="MSDSSESPSFYLLRGDDSTRFRELVAGFKDALGDPDTADLNTTL
                     LDGETLNIDSLTADVMAMPFLAPRRLVIVENARSFLTKQVKNAKDRFLKLLAELPQTT
                     QLVVFVDDQIIKRGGKAYWENVKSYDWVLEWIGQNPSSAQVMNCSLPDVADMPVWIRR
                     NCKDKGGTIDKSASALLAQYVGNDTLRAGHEIDKLIAYAGEGGTITTKDLVLLTTQDQ
                     EGDIFALVDALGERNGKKAMEQFLLLTEKSDVMELTGMIHRQFRLLIQAREVMDEGGR
                     QDKVQSELKVLSFIAGKLYKQAGRFSMAQLLDIYARLLKIDEDIKTSGMPGIIAFQVL
                     IADLTL"
     gene            2361..4739
                     /locus_tag="PK791_00100"
     CDS             2361..4739
                     /locus_tag="PK791_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91746.1"
                     /translation="MGCGSKLEQSAPVEPVAEESIQEPVGTEQEVPAQSTDSDGFVDA
                     YIPPAPGPVLYSPPPADTPQTVPQQVGPQQGEFVSAPSQASFGPGQAVPPGFQQAPPP
                     PIYQAPPKKKSNALWIILGVVGGLLILCVILFFAVIRPIINRTKDIIEGEMPNVMETI
                     ISESVEETPEVLPTEAEVPLPTEPAGGSLIGSDTVWEGFPATAEEITAENAYRMEQGM
                     RFGDGYSLAISYSPDGKYLAVATSIGVDIMDPNSGQKVKRLLDDEPVYDLIFAPNGDL
                     LASTYDGIYHFTADSFESISMFAENAINYEMTLSSDGSILASDYYDVALIFKKTGQTY
                     TYSHEIIDGEDYYVIGISPDGTKLASGNSDGELKIWDVDSGNLLKTINAHTDYINQLA
                     WSPDSSLIATASDDDSLAIYNISSGEKVFSNSFDSTNGTAVVFSADGTKLIANGDYSE
                     IAVWDINAGSLLKTFTWGDTYFTNLELNTANTSIFARNDYGQLILWDYETNQQTELAV
                     NYIDAYYSLAISPDGSQYFYLDNNDLSMMIGENGEVVWSRVDERFDQMLAPAFSKDGT
                     MLLGGTYHGYFNFLKANNGTELDYFDAHDDWIRDIAISPDESKIATASDDETVKVWDF
                     NTFELLFTLEGHTDYVRTVAFSPDGQYIASGGDDETVRIWDANTGASLHVLEGHENWV
                     FAVAFTPDGSMLGSTGSDDFVLFWDPVTGNQIRKIKTSVSTKYSLEYTKDGKAFWAQD
                     GREVKLYDIEDGTVLVTLSGHTGNIVRVKMSADGTTLVSAGYDGTIRVWRVK"
     regulatory      4795..4897
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4925..5581
                     /locus_tag="PK791_00105"
     CDS             4925..5581
                     /locus_tag="PK791_00105"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOH91747.1"
                     /translation="MKKEKRAILFSNGEAQNPERLQIRTDDYLIAVDGGLKHLEKLGI
                     SPDLLIGDLDSVDSQDLQNLESSSVEVLRFNPQKDDTDLALALREAIKRGFQRIVIAF
                     GLGGRVDHLLASLGLLEAAKTEAIEIYFDDGTTRVFLFDDEIMLDAEPQDLISLIPWR
                     GDTLVHETQNLAYALRDEWLPFGTSRGVSNLALAQTIRVSLSKGELLVIHTKTNADKA
                     "
     gene            5593..6657
                     /locus_tag="PK791_00110"
     CDS             5593..6657
                     /locus_tag="PK791_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOH91748.1"
                     /translation="MMKKLLLILTIFILLFAACAPDTNNLSASRVLNVMTHDSFDITA
                     ELLAKFERENNVRVNIIKAGDAGSALNRLILTSTAGASEADVFYGLDNSFLSRALENE
                     IFEAYQAPALTHIDASFQLDTSNRALPINYGDVCINYDKAWFADHGLAVPTSLEDLTL
                     PAYQGLLAVENPSTSSPGLAFMMATIAHFGEDGWLDYWKQLKDNQVVIAADWETAYYS
                     NFSGSSGKGAQPLVVSYASSPVAEFIYAEVELDEAPTASIVAEHACWRQIEFAGIVKG
                     TQQRALAEKFIDFLLSKDFQEDLPLQMFVYPVLPEALLPDEFTRASQVPEKPATLDPA
                     DIAKNRELWVEQWLELMLSN"
     gene            6705..8393
                     /locus_tag="PK791_00115"
     CDS             6705..8393
                     /locus_tag="PK791_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOH91749.1"
                     /translation="MKRSIWQTLLLWVLPLAFLLIFFYQPLLAIFRLVFSAQFSQGWK
                     LFRWSQITKPLAFTLYQATLSTVLTLVLGLPAAYLFARFDFAGRKFLRVLITIPFILP
                     TVVVTAAFNTLLGPRGWLNLGLMSLFNLSAPPIHMLNSLSAILLAHIFYNTSIVIRMV
                     GSNLSRLRQNLPLAAQNLGASPWRAFKEVTLPLLMPAILSATLMVFLFDFTSFGVVLM
                     LGGPQFSTLEVEIYNQAMNRFNLPVAGLLSIVQLAFTLLVSVLINRSKQPRYGKAALA
                     SESSGLREARTRWEKIFVIVMVVVLLLLMVTPTLSLLTRSFFTFDAARGERGELKTGF
                     TLRYYRELFENREQSLFYVPPILAIRNSMIYAGITMLLATVLGLISVYAIQKSGKLSR
                     FLEPLIMLPLGASAVTLGLGFLIVFGGTVLTQGRFQALIPVAHTLVALPMVIRTLLPA
                     LRGIPENLRLAAKSMGASPLRVFREVDLPVLFRAIAVSLLFAFTISLGEFGASSFLSN
                     TAMPTIPVAIYRYLSQPGALNYGQALAMSSLLMVVCVAGSLIIEQIRLPGEEVF"
     gene            8393..9400
                     /locus_tag="PK791_00120"
     CDS             8393..9400
                     /locus_tag="PK791_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH91750.1"
                     /translation="MLSVRDIYKTYEGAPLLNGLSFDLAQGEIIALLGTSGSGKSTLL
                     RIIAGLEEAESGAVYWDGVDLAQTPAHQRGFGLMFQDYALFPHRNVYENIVFGLRMQK
                     RPEAEIEKIVGERLKSIRMESFAKRPVTELSGGEQQRVALARALAPDPRLLMLDEPLG
                     ALDRGLREELTKELRQTLRDSHKPVIYVTHDRQEAFGLADRLLILHDGVIVQDGSPQE
                     LFERPANAWVAEFLGLGNLISANDLTKNAIQEEKPKASDDDRWLLRPFDAQIGESQTD
                     TSFQFEGEILDCVYMGERYQTGLRTAQGTVLSIETPHLCVMGEKIKVYWPAEKVQCLK
                     S"
     gene            9385..9873
                     /locus_tag="PK791_00125"
     CDS             9385..9873
                     /locus_tag="PK791_00125"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOH91751.1"
                     /translation="MPQKLNLHIPNPARQLKIIKHHAGSEKIYTWSGQLLSGTPNSRR
                     VNACFNGNPGYAGKVLLEPGDRFVESYFTDCWYNFFQIYAGQSNLIKCLYFNISRPAV
                     FYDDRIEWEDLSLDLLVYPDGLKQLLDLDEFALLNIDEKTRQICWQTISDLANTDLTC
                     LL"
     gene            complement(9905..10354)
                     /locus_tag="PK791_00130"
     CDS             complement(9905..10354)
                     /locus_tag="PK791_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HOH91752.1"
                     /translation="MSNNFDPNIPPQEQAPQQAPQGPAPYQAPQQPPQPAPYQTLPPP
                     PPPAPKKSYSVWAIVSLVAGILNFKFAPFGAIAALVTGYVARNEIRESNGTLEGQGMA
                     IAGIVLGWVGLAFSIIMVVLSVLFMVGMITSTPLVCGPFFEWLHSLP"
     gene            complement(10338..10949)
                     /locus_tag="PK791_00135"
     CDS             complement(10338..10949)
                     /locus_tag="PK791_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOH91753.1"
                     /translation="MGGTLTGIFSVLSAFGLSASAGLNAYIPLLVVALLAKFTNTITL
                     ASPFDALESWWTIGVLIVLSVVEIVADKVPAVDHVNDIVQSVIRPVAGAIIFAASANI
                     ITDISPVLSLICGLLVAGSVHATKALVVRPAVNVSTAGVGAPVVSTAEDASAVAISVL
                     SVVAPILMVILVFLLIWLIAWIVRKRRSKKEARLKKENYEQQL"
     gene            complement(11038..11859)
                     /locus_tag="PK791_00140"
     CDS             complement(11038..11859)
                     /locus_tag="PK791_00140"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOH91754.1"
                     /translation="MKQDDSTRQDNPKRSRVWLILLLVLICVLIGLWIWFTPSGFWGK
                     VNALGYSVCHQITPRSFVLGDSQSPLCARCAGMYLGALVTLIAHFIWGKHGKFPPLWI
                     LIFLGLGLLAFALDGLNSAAGLILPSVSFYETTNLTRLITGLAVGLGIGSVLAPIFNQ
                     TLWHKWLSSSAYEKGYRFPLLIAVLGIVGWAVYSQLPVFFYVSNLLMGLGILLTLWVI
                     HTTLASFILKRNNTVKQWRDLVLPGLLGFVMVLAQIGLISLARYLLTGTWEPVVL"
     gene            complement(11876..13024)
                     /locus_tag="PK791_00145"
     CDS             complement(11876..13024)
                     /locus_tag="PK791_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOH91755.1"
                     /translation="MEYRNFGKLDWKVSALGFGTMRFPVIDGDSSKIDEPTAAKMLRY
                     AIDNGVNYVDTAWGYHREQSEPFVGRVLADGYRERVKLATKMPSWLVKEPGDLDKHFN
                     TQLERLQTDYIDCYLLHALNKRYWDNYLSLNIFDWLERKKDEGKIRAVGFSFHDSYEV
                     FEEIINGYDHWDFCQIQYNYVDIEEQAGVKGLKLAADKGLAVVIMEPLRGGGLASPVL
                     PPSVAEVFARSEHPWTHIEWALQWLWDQPEVSVVLSGMSTPEQVEQNVQFASRSGVGK
                     LSDDDRQLISDAHQAMRACSPIPCTKCEYCLPCPQGVRIPSIFAIYNDAMMFNLQAYG
                     QRNYGFSVPKDGRADQCIECGACEALCPQQIEIIESLKVAHKYLTETI"
     gene            13302..14726
                     /locus_tag="PK791_00150"
     CDS             13302..14726
                     /locus_tag="PK791_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOH91756.1"
                     /translation="MDSNKAIPESEEKPTPSSVEKPRSSRGAVSPPWSWQIKLVVVVL
                     LLGGAIALAVKYNNYLQLFLTAMLISFLIQPVVRWIHKHTKMNWKLISALLYLIILGL
                     LVWGLFSGGRGLVGQITGLFKTLQENVGALTDFLAGLSNQTVHIGPLEFKTPDINTKF
                     ITEKINEFVQPLVGEITKNMGRMLGSVGSFLFNLVTVILVSFFITAESGGQKKPFLNI
                     KNQEFNYDLRRMGREISGNFNAFLRGTFTVVLIAIVVYSALLGALGVPYYFLLAVIAG
                     FGRFIPYLGAFIGWVGFAIGALIQNPTPFGLTPFVYMLLVLGVSLFIDVILDHLVTPS
                     VMSEVLDVHPAAILISALVGGQMFGLLGVILAAPAYAVLTLLVRYLVRKLFDEDPWEG
                     MVYYRKPPEAGVIKFLRKWGKRFWQWTEKPRQAISAWFVKLWGKVAGFFKKLFAKLPK
                     SKRKKQDKGKEESPDLDSDGKAQE"
     gene            14806..14997
                     /locus_tag="PK791_00155"
     CDS             14806..14997
                     /locus_tag="PK791_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91757.1"
                     /translation="MTNSQDPITSTIAETENYLAWKAEEPDGETTYHLELNNVTLHFF
                     KEEWDEILQLLEIIVDEEL"
BASE COUNT         3785 a         3561 c         3748 g         3971 t
ORIGIN      
        1 cccactaatt ttggcaaccc gcgccgtatc gcgaatggca tcaacttcaa tcgcttgctg
       61 attatcgtgg ctgttttaac gcgtcgcgtg ggccttaatt taggtaatca agacgtgata
      121 accaacgtgg tgagtggctt aagactcgaa gaacccgcgg ctgatttggc tgtggcgacc
      181 gctttggcgt catctatgcg cgacttgccc acacgcgcgg ataccgtgct aattggtgag
      241 gttggccttt ccggcgaact gcgtatggta gggcaaatgc caacacgtct gcgcgaagcc
      301 gcgaagctgg gctttaaacg agccatcgtg ccccgacgcg cgcgcaaagg cgaaccctgg
      361 cccgatacca ttgagatttt agaggcacgt tccttgcgcg acgcgctcaa actggcgttt
      421 gttgaagagg aaaaataagt accacagata tgccctgaag tgtatctgcg cattttggta
      481 aaaagtttaa taatgcagcg ccaaaataat caatagcttg gtagaatcag gatatagtct
      541 aaaagactta accatcatgc cagcggaaga aatcgttaaa cgctacgatt ccttagtata
      601 aaagaaggtt gcaatgaaaa agaacatact cgattacatt cccatcatcc gtcgtattcg
      661 acgtaatcac gctttggaac atgccacaat gcatgtgctt tcagagcgct atccgggttt
      721 gcgaatgatg ggcatttcaa acccgaaagg ttttctgatg gttgccgact tgcccaccga
      781 actgatgacc gaagccacac tggaggctaa gaatcgtttg aaagcgggtg aaacagattt
      841 ggcagtacat ccaaattgcg gatcgaatat cgccgttccc accttggtgg caagcatggt
      901 cgcgggcagc gttttggctt taagcactgt caacgaaaag aaaccgaagt ggtatcacta
      961 tctgattgcc gctgcgtctg ctgtccccgc ctattttctc gcccggcctt tgggtccgat
     1021 tactcaaaag tacatcacca ccagtggcga aattgaagac actcaagtta agggtgtact
     1081 aacccaaaag gttttgaatg gatcggttca taaagtagat actgaggcat aacaaatgtc
     1141 tgattcgtct gaatcaccaa gtttttatct cttgcggggg gatgacagca cacgttttcg
     1201 tgagctggtg gcaggtttta aagatgcttt aggcgatcca gataccgctg atctcaacac
     1261 cactctcttg gatggcgaga ctttaaatat tgacagtctg actgccgacg tcatggcaat
     1321 gccttttttg gcaccacgtc gcctggtaat cgttgaaaac gcgcggagtt ttttgacgaa
     1381 acaagtcaaa aacgccaaag accgctttct gaaattgctt gcagaacttc cccagaccac
     1441 gcagttggtc gtgtttgttg acgaccaaat cattaaacgc ggtggcaaag cttattggga
     1501 aaacgtcaaa agctatgact gggtactgga atggatcggg caaaatccgt ccagcgcgca
     1561 ggtgatgaat tgttccctgc ccgatgtcgc cgatatgcct gtgtggattc gacgcaactg
     1621 caaagataaa ggtggcacga tagataagtc cgcttccgcg ttgcttgcac agtatgtggg
     1681 taacgacaca ctccgtgccg ggcatgaaat tgacaagctc atcgcctatg ccggtgaagg
     1741 tggcaccatc acgaccaaag atcttgtttt acttacaacg caagaccagg aaggggatat
     1801 ttttgccttg gtggatgcct tgggcgaacg caacggcaaa aaggcgatgg agcaattttt
     1861 gttgcttacc gaaaaaagtg atgtcatgga gcttacaggc atgattcatc gccagttccg
     1921 cctgcttatt caagcccgcg aagtgatgga tgaaggcggt cgtcaggaca aagttcagag
     1981 tgagctcaag gtattatctt ttatagcagg caagctttat aagcaagccg gtcgcttttc
     2041 gatggcgcag ttgcttgata tttatgcgcg cttgttaaaa attgacgaag atatcaaaac
     2101 gagtggtatg cccggcatta tcgctttcca ggttctgatt gctgatttaa ctttatagcg
     2161 tattgagtgc ctttactcaa aattttcacg caatagattt gcgtttttta ttaaactttt
     2221 acccagtttt cctagccgtt tgtattgttt cttagtagaa tagattagat gacaaacact
     2281 aaactattgc cacgaatgag gaggtattga tgatttgtcc atattgcaat acaaacaata
     2341 aacctggcag caatttctgc atgggttgcg gtagtaaact ggagcaaagt gctcctgttg
     2401 agcccgttgc ggaagaaagt attcaggaac cagtgggtac agagcaagaa gttcccgcgc
     2461 aaagtactga ttcagatggg tttgtggacg catatattcc acccgcacct ggacctgtgc
     2521 tctactctcc acccccagcc gacacacccc aaactgtgcc acaacaagtt gggccacaac
     2581 aaggcgaatt tgtgagtgcc cccagccaag ccagttttgg acctgggcaa gcggttccgc
     2641 caggattcca acaggcaccc ccgcctccca tctaccaggc gcccccaaaa aagaaaagta
     2701 atgctctttg gattatcctg ggcgttgtgg gtggtttgct gattctttgt gtgattctgt
     2761 tctttgctgt tattcgaccg atcatcaatc gcaccaaaga cattatcgaa ggtgaaatgc
     2821 caaatgtcat ggagacaata atcagtgaat ctgtggaaga aacacctgaa gttttgccta
     2881 ctgaagctga agtccctctg ccaacagagc ccgctggcgg cagtcttatt ggttcagaca
     2941 ctgtctggga aggtttccct gcgacggcgg aagaaataac cgctgaaaat gcctatcgta
     3001 tggaacaagg catgcgcttt ggcgatggct acagtttggc gattagctat tcgcctgatg
     3061 gcaaatatct tgctgttgcc acgtcaatcg gtgttgatat catggatcca aacagcggac
     3121 aaaaagtgaa acgtttgttg gatgatgagc ctgtttatga tctcattttc gctcctaatg
     3181 gtgatcttct cgcatctacc tacgatggca tataccattt caccgccgac agtttcgagt
     3241 caatctccat gtttgctgag aacgccatta attacgaaat gaccctttca agcgatggga
     3301 gtattctggc ttctgactat tatgacgttg ccttgatttt taagaagact ggccaaacat
     3361 acacttactc gcatgagatc attgatggcg aagattatta tgtgattggt atttcgccag
     3421 atggcaccaa actcgcctcc ggaaacagtg atggcgaact caaaatctgg gacgtcgact
     3481 ctggcaatct cttgaaaaca ataaatgccc acacagatta catcaatcag ttggcctggt
     3541 cacctgattc cagtttgatt gctacagcaa gtgatgatga cagtttggcg atctataata
     3601 tttcttcagg cgagaaagtc tttagcaaca gcttcgacag cactaatggt accgcagtgg
     3661 tgttttccgc agatggcacc aagttgatag caaatggtga ttattcagaa attgccgttt
     3721 gggacattaa cgctgggtca cttctcaaga ctttcacctg gggagatacg tacttcacaa
     3781 accttgagtt gaatactgcc aacacaagca tcttcgcgcg caatgattat ggtcagctta
     3841 tcctttggga ctatgaaacc aaccagcaaa ccgagttggc tgtaaactat attgatgcct
     3901 attattcact tgcgatttcc cccgacggct cacaatactt ctatttggat aacaatgatt
     3961 taagcatgat gataggcgag aacggcgaag ttgtctggtc gcgggtagat gagagatttg
     4021 accaaatgct ggcgccggct ttcagcaaag acggtacgat gctgttgggc ggtacctatc
     4081 atggttattt caatttcctc aaagcgaaca atggcacgga acttgattat ttcgatgcgc
     4141 atgatgactg gattcgtgat atcgcgatat ctcctgacga gagcaaaatt gccactgcca
     4201 gtgacgatga aaccgtaaaa gtgtgggatt tcaatacctt tgagcttttg ttcacacttg
     4261 aaggtcacac tgattacgtg cgtacagtag ctttttcgcc agatggccag tatattgcca
     4321 gtggcggtga tgatgaaaca gttcgcatct gggacgcgaa caccggtgcc agtttgcacg
     4381 ttttggaagg gcacgaaaac tgggtgtttg cggtagcctt tacaccagac ggaagcatgc
     4441 tgggcagcac tggctcggat gattttgtgc tcttctggga tcctgttacc ggcaatcaga
     4501 ttcgtaaaat taaaacaagt gtatctacaa agtacagtct cgaatataca aaggacggca
     4561 aagcgttttg ggcacaagat ggcagggaag taaagcttta tgatattgaa gatggtaccg
     4621 ttcttgtgac gctctccggg cataccggta atattgttcg cgtgaaaatg agcgcagatg
     4681 gcactaccct ggtgagcgct ggctacgatg gtacgatccg cgtttggcgt gtgaaataag
     4741 cctaaacaga taatagattt tgtttgaatt atgtaaggaa tgtgttatca ttccttacat
     4801 aacccgggga gctcgcaagg gctgagaggg accaaaagtc cgacccgtaa acctgatccg
     4861 gataatgccg gcggagggat ggagcaaaaa cgttaaacac cctccctggc gagggtgttt
     4921 tgttatgaaa aaagaaaaac gtgcaatttt atttagcaat ggcgaagcgc aaaatccaga
     4981 gcgactgcaa attcgcacgg atgactatct catcgctgtt gacggtggcc tgaagcatct
     5041 tgaaaaactg ggaattagtc ctgatttgct tatcggcgat ttggactctg ttgattcaca
     5101 agatctccaa aaccttgaaa gcagctcggt agaagttctg cgttttaacc cccaaaaaga
     5161 tgataccgac ctggcacttg ccctgcgcga ggcgataaag cgtggatttc aacgcatcgt
     5221 cattgccttt ggcctgggcg gacgtgtgga ccatctatta gctagcctgg gcttgctcga
     5281 agcagcaaaa accgaagcca tagaaattta ctttgatgat ggcacaacac gcgtattttt
     5341 gtttgacgac gaaataatgc ttgatgccga gccccaggat ttaatttcgc taatcccctg
     5401 gcgcggtgat acgctggtgc atgaaaccca aaacctggcc tatgccttac gcgatgaatg
     5461 gctgcctttc ggtacaagcc gtggcgtcag caatcttgcc cttgcgcaaa ccatacgggt
     5521 cagtttatcg aaaggcgagc ttttagttat tcacacgaaa acaaatgctg ataaagcata
     5581 gttgaaagga aaatgatgaa aaaacttttg cttatactca cgatattcat actgctcttc
     5641 gctgcatgcg cgcctgatac aaataattta tcagcaagtc gggtgctgaa tgtgatgacg
     5701 catgattcct ttgatatcac tgcagaactc ctggcgaaat tcgagcgtga aaacaatgtc
     5761 cgtgtcaaca ttatcaaggc tggcgatgcc ggcagcgcgc tgaaccgtct tatcctgacc
     5821 agtactgcag gcgccagcga agcggacgtt ttctatggcc ttgataacag ttttctttcc
     5881 cgcgccctcg aaaacgaaat tttcgaggct tatcaggcgc ctgctctgac gcatattgat
     5941 gccagttttc agctggatac atccaaccgc gccttgccga ttaactatgg cgatgtctgc
     6001 atcaactacg ataaagcctg gttcgcggat catggactgg cagtacccac ttcgcttgaa
     6061 gacttgaccc tgcctgctta tcagggcctg ctggctgttg aaaatcccag tacttcatca
     6121 cctggcctgg cgtttatgat ggcgaccatc gcccatttcg gtgaggatgg ctggcttgac
     6181 tactggaaac aactcaaaga caatcaggtc gttattgctg cagactggga aaccgcctac
     6241 tacagcaatt tttcaggctc ttcgggcaag ggggcgcaac cgctggttgt gtcttatgct
     6301 tccagtcctg tcgcggaatt catttacgcc gaagtagaac ttgatgaagc gccgacggct
     6361 tccatcgtgg cagagcatgc ctgttggcgt caaattgagt tcgctggcat cgtgaaaggc
     6421 actcagcagc gcgcgttggc tgaaaagttt attgactttc tcttgagcaa ggactttcag
     6481 gaagacttgc ccttgcaaat gtttgtttat ccggtattgc cggaggccct tttgcccgat
     6541 gaatttacgc gggccagcca agtccctgaa aaacccgcca cgcttgaccc tgccgatatt
     6601 gccaaaaacc gtgagttgtg ggttgaacag tggcttgagt taatgttaag caactaagcg
     6661 aatgtttggt tggattattg aaaggacata aagagaagta caagatgaaa cgctccattt
     6721 ggcaaacact attgttatgg gtgctgccac tcgcgttttt gctgattttc ttttatcagc
     6781 ccttgttggc gatcttccgt ttggtctttt ctgcccagtt tagtcagggc tggaaattgt
     6841 tcagatggtc gcaaatcacc aaaccacttg cttttaccct gtaccaggcc acactttcca
     6901 ctgtgctgac gcttgttctg ggactgcccg cagcttatct gttcgcgcgc tttgattttg
     6961 cagggcgcaa gtttttgcgg gtgctgatta ccattccgtt tattctgccc acagtcgtcg
     7021 tgactgccgc gtttaacaca cttttgggtc cacgtggctg gctgaatttg ggcttgatgt
     7081 ccctgtttaa cttgagcgca ccgcctattc atatgctcaa cagtcttagt gccattctgt
     7141 tggcgcacat tttttacaat acgtcaattg tgattcgcat ggttggttct aatttaagtc
     7201 gcctcaggca aaacctgcct ttggcagcgc aaaatttggg cgcgtctccc tggcgggctt
     7261 ttaaggaagt aaccttaccc ttactgatgc cagctattct tagcgcgacg ctcatggttt
     7321 ttttgtttga ctttaccagt tttggcgtgg tgctcatgtt aggcggtccg caattctcaa
     7381 cgcttgaagt ggaaatttac aaccaggcta tgaaccgctt caatttaccg gtagcgggct
     7441 tactttcaat tgtgcaactt gcttttacgc ttttggtgag tgtgttaatc aatcgcagta
     7501 aacaaccgcg ttatggcaaa gcagctttgg cgtctgaaag ctccggtttg cgcgaagcca
     7561 gaacacgctg ggagaaaatt tttgtcatcg tcatggttgt tgttttactt ttactgatgg
     7621 taacgccgac cttgagtttg ttaacgcgtt cctttttcac ctttgacgcg gcgcggggtg
     7681 agcgtggtga actaaaaaca gggttcactt tgcgctatta ccgcgaactt ttcgaaaatc
     7741 gggagcaatc cctgttttat gtacccccca ttctcgcgat tcgcaactct atgatttacg
     7801 ccggcataac catgctgctg gcgacggtac tggggctgat atcggtttac gctattcaaa
     7861 agtcgggcaa gctgagccgt tttttggagc cccttatcat gttgcctttg ggtgccagcg
     7921 cggtgacgct tggtctgggc tttttaattg tctttggtgg cacagtgctt acccaggggc
     7981 gttttcaggc gctcattcct gtcgcccata ccttggtcgc tttgcccatg gtcatccgca
     8041 cgcttttgcc tgccttgcgg ggaattccgg aaaatctgcg tctggcagcg aagagtatgg
     8101 gcgcgagccc tttgcgcgtg tttcgggaag tcgatttgcc tgtccttttt agagcgatcg
     8161 cggtgagttt gcttttcgct tttacgattt cactgggtga atttggcgca tcaagctttt
     8221 tgagcaatac tgccatgccc acgattccgg ttgccatcta tcgctatctt tcccaacccg
     8281 gcgcgttgaa ttacgggcag gcactggcga tgtcgagtct tttgatggtg gtttgtgtgg
     8341 caggcagttt gataatcgag cagattcgtc tgccgggtga ggaagtgttt tgatgttaag
     8401 cgtacgtgat atttataaaa cctatgaagg cgcgccctta ttgaacgggc taagctttga
     8461 tttagctcag ggcgaaatca ttgccctttt gggcacatca ggcagcggta aaagcaccct
     8521 gttacgcata attgccgggc ttgaggaggc ggaatccggc gcggtttact gggatggtgt
     8581 tgaccttgcc caaacacctg cccatcagcg tggttttggt ttgatgttcc aggattacgc
     8641 cctctttccg caccgtaatg tttacgaaaa tatcgttttt ggcttgcgta tgcaaaaacg
     8701 ccctgaagcg gagattgaaa aaatcgtggg tgaacggctc aaatcaatcc gcatggaaag
     8761 cttcgccaaa cgacctgtga ccgaactttc gggtggcgaa cagcaacgcg tcgctttggc
     8821 acgtgccttg gcacccgacc cgcgcctctt gatgcttgac gaaccgcttg gcgcgcttga
     8881 ccgtggcttg cgcgaagaac tcaccaagga actgcgccaa accttgcgcg acagccacaa
     8941 gccagttatc tatgtcaccc acgaccgtca ggaagctttt ggtctggcgg accgtttgct
     9001 catcttgcat gatggggtga ttgtgcaaga cggcagtcca caggagctct ttgaacgccc
     9061 ggcgaacgcc tgggtagcag agtttttagg cttgggcaat ctcatcagcg cgaatgattt
     9121 gacgaaaaac gccattcagg aagaaaagcc caaggcaagc gacgatgacc gttggctatt
     9181 gcgtcccttc gacgcgcaaa tcggtgaatc tcagacagat acctcctttc aatttgaagg
     9241 tgagattttg gattgtgtct acatggggga acgataccaa acagggctgc gtaccgcgca
     9301 aggaactgtg ctatccatcg aaaccccaca tttgtgcgtg atgggcgaaa aaattaaggt
     9361 ctactggcca gcggagaaag ttcaatgcct caaaagctaa acttacacat tccaaaccct
     9421 gccagacaac tcaaaatcat caaacatcac gctgggtcag aaaagatata cacctggtct
     9481 ggtcaactgc ttagcggaac gccaaatagt cgccgtgtta acgcttgctt caatggcaat
     9541 cctggctatg ctggcaaggt cttgcttgag cctggcgacc gttttgtgga atcttacttc
     9601 accgactgct ggtacaattt tttccaaatc tatgccggac agagcaacct aataaaatgt
     9661 ctttacttca acatatcccg tccggctgtt ttctacgatg accgcattga atgggaagat
     9721 ttgtcacttg atttgctcgt ctatcctgat ggtttaaagc aactattaga tttggatgaa
     9781 ttcgctcttt taaacattga cgaaaaaaca cgccaaatct gctggcagac catttctgat
     9841 cttgcaaaca cagacttgac gtgtttactt taattcgagc taaatattag ccagttttcc
     9901 tgaactaagg cagtgagtgt agccattcaa agaatgggcc acaaaccagt ggggtgctgg
     9961 taatcatgcc gaccataaac aaaacgctta gaaccaccat gattatgcta aatgccagcc
    10021 ctacccagcc taagacgata ccggcgatag ccatgccctg gccttccaaa gtgccgttgc
    10081 tttcgcggat ttcgttgcgc gccacgtaac cggttaccag agcggcaatg gcaccgaaag
    10141 gcgcaaactt gaagttcaga atgccagcga ccaggctgac aatcgcccaa acggaatacg
    10201 attttttggg agccggcgga ggagggggtg gcaaggtttg atagggtgcc ggttggggcg
    10261 gttgttgagg cgcctgatat ggcgctgggc cctggggagc ctgctgtggg gcttgttcct
    10321 ggggtgggat gtttggatca aagttgttgc tcatagttct ccttttttaa tctagcttcc
    10381 tttttgcttc ttcgcttcct tacaatccag gcgatgagcc aaatcagcaa gaaaacgaga
    10441 ataaccataa ggatgggggc tactacagat agtacggata tggcgaccgc tgaagcgtct
    10501 tcggcggtac taacgacggg cgcgcccacg ccagcggtgc tcacattgac ggcgggtctg
    10561 accacaagcg cttttgtggc gtggactgaa ccagctacca gcaaaccaca aatcagggac
    10621 agtaccgggc tgatatcagt gatgatgttc gcgctggcag cgaagataat tgccccggcg
    10681 actgggcgaa tgacgctttg aacgatgtcg ttcacatggt caaccgcggg gactttatcg
    10741 gcgacaatct ctacaactga caaaacgatt aagacaccga tggtccacca gctttccaaa
    10801 gcgtcgaaag gcgaggccag cgtgatggtg ttggtgaact ttgccagcaa agcgacaaca
    10861 agcagtggga tataagcgtt aagacccgct gaagctgaaa gaccaaacgc gcttaatacg
    10921 ctaaaaatgc cagttagggt tccacccata attgctttcc tttcaaataa gcattaatca
    10981 cactactatt atagactttc tgtataagaa aaacagtaga taatctatgg aatctcgcta
    11041 cagaacaaca ggttcccatg ttccggttaa caaatagcgc gccaggctga taagaccgat
    11101 ttgcgctaag accatgacga aacccagcag tcccggcaga accaaatcgc gccattgttt
    11161 gaccgtgttg ttgcgtttca agataaagct tgccagggtg gtgtggatga cccaaagggt
    11221 gagtaatata ccaagcccca tcagcaaatt tgatacataa aagaagaccg gcaactgact
    11281 gtataccgcc cagcccacaa tgccaagtac tgctattaat aaaggaaagc gatatccctt
    11341 ttcgtaagcg gaactggaca gccacttgtg ccacagggtt tggttgaaga taggcgctaa
    11401 aacagagccg attcccaagc caactgccag tcctgttatc aaacgggtga gattagtcgt
    11461 ttcgtagaag ctgaccgatg gcaaaatgag cccggcagcg gagttgagcc cgtccagtgc
    11521 gaaggccaaa aggcctaatc ccaaaaaaat cagaatccaa agtggcggaa acttgccatg
    11581 tttgccccag atgaagtggg caatgagcgt gaccaacgcg cccaggtaca tccccgcgca
    11641 gcgcgcgcag agaggcgatt gagaatcgcc caacacaaag gaacgcgggg taatttgatg
    11701 gcaaacactg taacccagcg cgttaacctt gccccaaaag ccactgggcg tgaaccaaat
    11761 ccacagcccg attaacacgc aaatcagcac taaaagcagg ataagccaaa cgcgtgaacg
    11821 ctttggatta tcctgcctgg tggaatcatc ttgtttcata tcttgtgaca aggcgctata
    11881 tcgtttcggt tagatactta tgcgcgactt tgagggattc aataatctca atctgctgtg
    11941 gacaaagtgc ctcacaagcc ccacactcaa tgcactgatc agcgcgtccg tccttaggta
    12001 ccgaaaaacc ataattgcgc tggccatatg cctggagatt aaacatcatc gcgtcgttat
    12061 agatcgcgaa aatgctggga atacgcaccc cttgcgggca aggcagacaa tactcgcatt
    12121 tggtacaggg tatcgggctg caagcgcgca tcgcctgatg ggcgtcgcta atcagctgac
    12181 ggtcatcatc gctaagctta cccacgccac tgcgactggc aaattgaacg ttttgctcaa
    12241 cctgttcggg ggtagacatg cctgaaagca ccacagaaac ttcgggctga tcccaaagcc
    12301 attgcagcgc ccactcgatg tgagtccatg ggtgttccga gcgggcaaaa acttccgcga
    12361 cgcttggtgg taaaacgggg cttgccaacc ccccaccgcg caatggctcc ataatgacga
    12421 ccgccaaacc cttgtcggcg gccagtttaa gcccttttac gccagcttgc tcttcgatat
    12481 ccacataatt gtattgaatt tggcaaaagt cccagtggtc gtagccattg atgatttctt
    12541 caaagacttc gtaactgtcg tggaacgaaa aacctaccgc gcggattttc ccttcgtctt
    12601 tctttctctc aagccagtca aagatattaa ggctcagata gttatcccag tagcgtttgt
    12661 taagcgcgtg caaaaggtaa cagtcgatgt agtcggtttg caaacgttca agctgggtgt
    12721 tgaagtgttt gtctaaatct ccgggttctt taaccagcca cgaaggcatt ttggtggcta
    12781 gttttacgcg ttcgcgataa ccatccgcca ggacacgccc tacaaagggc tcggactgct
    12841 cgcggtgata gccccaggcg gtgtcaacgt agttcacgcc attgtcaatg gcgtaacgca
    12901 gcatcttcgc tgcggttggc tcatcaattt tgctgctgtc gccatcaata acaggaaaac
    12961 gcatcgtgcc aaagccgagt gcgctcacct tccagtctaa ttttccaaaa ttccgatatt
    13021 ccattgagca ctcctttact atatgacttg gtttaatttt acccgtttat cccgtatggt
    13081 gacggtgatt tagcataagc ttcttatccg ctttattaac tttgttctta gcgcgcaaat
    13141 ttcgttcccc ggtttgtaaa acatttatca gatgttttcc tcttatgcaa aaacctgatg
    13201 ataaagcctt gattcgagtg gttgattgtg gtttttttga taaaatgagt gcaggaacga
    13261 ttgaatcagc aaagtgcttg ctgaaaagaa aaaaggaagt tatggactca aacaaagcaa
    13321 tacctgaatc tgaagaaaag cccacgccat caagcgtgga aaagccacgt tccagtcgtg
    13381 gggcggtttc tccaccctgg agttggcaaa ttaagctggt tgtggtcgtg cttctgttag
    13441 ggggcgccat cgctctggca gtaaaataca acaattatct gcagttgttt ctcaccgcga
    13501 tgttgatttc ttttctcatc cagcctgttg tgcgttggat acacaaacac accaaaatga
    13561 actggaagct catctcagcc ttgctgtatt taattattct cggcttgttg gtttggggtc
    13621 tgtttagtgg cggacgtggc ttggtaggac agattacggg cttgttcaaa accttgcagg
    13681 aaaatgtggg ggcattaaca gattttctgg cgggtttgtc taatcagact gttcatattg
    13741 ggcctttgga attcaaaaca cccgatatta acaccaaatt tatcaccgaa aaaatcaatg
    13801 aatttgttca gcctttggtt ggcgaaatca ccaagaacat gggcaggatg ctgggttctg
    13861 tgggtagctt tttgttcaat ctggttaccg ttattctcgt ctcatttttt atcaccgccg
    13921 aatccggtgg tcagaaaaaa cctttcctga acattaaaaa ccaggagttc aattacgatc
    13981 ttcgccgaat ggggcgggag atatcgggta acttcaacgc ctttttgcgg ggcactttca
    14041 cggtggttct gatcgccatt gtcgtttact ccgctttgct gggcgcgctg ggtgtgccct
    14101 attatttcct gttggcggtg attgccggct ttgggcgctt catcccctat ctgggtgcct
    14161 ttattggctg ggtgggtttc gcgattggcg cgctgattca aaacccaacg ccatttggct
    14221 tgacgccttt tgtgtacatg ttgctggttt tgggtgtttc gctgtttatt gatgtgattt
    14281 tggatcacct tgttaccccc agcgtgatga gtgaagtgct ggacgtgcac cctgcggcga
    14341 ttttgatttc cgctttggtg ggcgggcaaa tgtttggtct tttgggtgtc atcctggccg
    14401 cgccggctta tgccgtgctc acccttctgg tgcgttacct ggtgcgcaaa ctgttcgatg
    14461 aagacccctg ggagggcatg gtttattacc gcaaaccgcc tgaagctggg gtgataaagt
    14521 ttttacgcaa atggggtaaa cgcttttggc aatggaccga aaaaccccgc caggcgatta
    14581 gcgcctggtt tgtgaagtta tggggcaaag ttgccggctt ctttaaaaaa ctcttcgcta
    14641 aactgccaaa atccaaacgc aaaaagcagg ataagggcaa agaagaatct ccggaccttg
    14701 attctgacgg aaaagcgcag gaataatccc ggtcaaaaag gctggctttc ctatccgcct
    14761 tgcattgcga tataattagg caaacgataa acaaaggagt aaattatgac taactcacaa
    14821 gatcctatca ccagcacgat cgccgaaacc gagaattatc tggcatggaa agccgaagaa
    14881 cccgatggcg aaacaaccta tcaccttgaa cttaataatg tcaccctgca cttctttaag
    14941 gaagaatggg acgaaattct gcaactgctt gaaatcattg ttgacgaaga actctagttt
    15001 taacgcctga cattgaaaat agctcttaat cagccagccc agagtgttag ggctggcttt
    15061 gtttt
//
LOCUS       DAOSFC010000003        14392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_3470_length_14392_cov_6.246146, whole genome shotgun sequence.
ACCESSION   DAOSFC010000003 DAOSFC010000000
VERSION     DAOSFC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14392
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3470_length_14392_cov_6.246146"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK791_00160"
     CDS             complement(<1..237)
                     /locus_tag="PK791_00160"
                     /inference="COORDINATES: protein motif:HMM:NF028055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91758.1"
                     /translation="MQELLQGLVIRSQAGWMTVRTSQGDYACRVRGRLRRGRAEGDLV
                     AVGDQVMVAPQADGTGRIDEVLPRHSVLSRKLSGV"
     gene            complement(250..2433)
                     /locus_tag="PK791_00165"
     CDS             complement(250..2433)
                     /locus_tag="PK791_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOH91759.1"
                     /translation="MKRLILIFVIFSLLLSACDPITKNTETPTPQVTATLPSPSVSRE
                     ELPDAEALAANYFQNWKIEDYNRMYEFLTIPAKAAYSLEDFIARHKKTAADLTMKSVD
                     FQILSNLLSTNAAQVSYHISYETNLIGTLERDTLMNLQLEGGEWKINWDPSMMLPELA
                     DGNYLELVLTIPTRGNIYSSDASNNYPLVAYTDAASLTIIPGKINPAQEDNLVSFLAE
                     LTNQTEDAVRLKYAYAQSNWSVVIGDISAEVAAENSSRIERFDGLYLQPFKSRFYYDN
                     GIAPHVTGYMLGISAEDQERYQRLGYKIDETVGASGLERWGEKYLAGKRGADLYVKNK
                     EGGIVTKLASTEAEPAQSITTTIDSRLQYWLQRSMGDRVGAVVVLERDTSKILAMVSS
                     PGFDPNIFNPLIHTHYEVSNLIQNVRQPLYNRAAQGVYAPGSIFKVISMISALETGVF
                     TPDRVYSCDNLWTEYDGWVGENWTYKRGFSADGDLTLVEGLMRSCNPWFWHIGLTLWN
                     DGYTTSIPDEALAYGLGTLTGIEIEEFAGAVNYPDNAFDYFQMAIGQSTLQVSALQAA
                     NLAAAIGNGGYLHRPTVIERIGFMGEEPVYQFTEPDLIRHIDTLPENLAAVQEGMVQV
                     IRNPRGTANFQFSRFSGNIAGKTGTAETALGKPHAWFFGYTFNEDPDKPDIAVAVILE
                     YGGEGSEMAAPLFRRAISLYYSDAEEPGGTMPWEKSPYQPLEEEE"
     gene            complement(2445..3137)
                     /locus_tag="PK791_00170"
     CDS             complement(2445..3137)
                     /locus_tag="PK791_00170"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOH91760.1"
                     /translation="MLESNPIMEEKRKLILASASPRRRELLALLGLPFDLRPGTYPEP
                     QKGEQSALDFVRALAVAKGQNVASSPGEIIFSADTIVLIDGKVLGKPADDEDARRTLD
                     TLRGRPHDVYTAINLFEVSSGDSWCEVCKTTVHMRQWSDEEQQAYIYKGTYHDKAGGY
                     AIQDDNFHPVERIEGCYANVMGLPLCLLYRMFKEKGLPLPANLPEACQAYTGTSCNYY
                     PDVLHEKVLQKV"
     gene            complement(3176..4099)
                     /locus_tag="PK791_00175"
     CDS             complement(3176..4099)
                     /locus_tag="PK791_00175"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOH91761.1"
                     /translation="MFELVFLGTSASAPSIRRGLSSLVVMHDEYRFMVDCGEGTQRQI
                     LRSGVGFKRLNHILLTHGHLDHILGLAGLLSTFMRWEAIEEVNIYGTQHTLDRVHDLL
                     YGVVLRGQEPGMPLNLIEVRPGPIFRTNKFEIRAFPVDHRGSSSLGYVFEEFPHRPFL
                     TDAAERLDIPPGPWRKDLVQGKEITLQDGRVISPDMVLGEARPGTKLSVVGDVGKPFK
                     LIDYVRDSDAIVTEATYLTEELDMAEKFGHSTAEQSAQLAKQANAKMLILNHVSRRYR
                     DDAILAEAQAIFPNTHLAADFDIFQIKARED"
     gene            complement(4116..4313)
                     /locus_tag="PK791_00180"
     CDS             complement(4116..4313)
                     /locus_tag="PK791_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91762.1"
                     /translation="MNKTGKTLIIIAGTLIGALVGVKVALNLVKEMEENEGRFPLSGS
                     ASLKAGLNAFNVMQQLTGKKG"
     gene            complement(4310..4780)
                     /locus_tag="PK791_00185"
     CDS             complement(4310..4780)
                     /locus_tag="PK791_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91763.1"
                     /translation="MDDEIKLKVERLHTEAQERQKKQRFKQIILPIIVVGVLLVIVLA
                     FAIVSALSDSSGIVGYKAGQITLIIVLFLALLVGFLLIYILFKTIDGLYKLNQTLPNY
                     GKIALGGVIQAEEGVRTVADASVEPVMTVQELGSKVKQVGESMKSHISPEGKQK"
     gene            5218..5676
                     /locus_tag="PK791_00190"
     CDS             5218..5676
                     /locus_tag="PK791_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOH91764.1"
                     /translation="MTKVITAITAQKRNTQRLNISLDGEYAFSLDRLTAAWLKVGRKL
                     SPEEIVSLQEKDEQEVAFNRALRYLSYRARSEAEMRKYLSDKGFSDHVSQTVIDRLKD
                     ERLINDPRFAQDWIDNRVSFRPRSQTQLRFELRNKGLSEDLSKMPCKKLI"
     gene            5682..5867
                     /locus_tag="PK791_00195"
     CDS             5682..5867
                     /locus_tag="PK791_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOH91765.1"
                     /translation="MELACVAGKKLVGRYARLGWLDFRQKLGAALARRGFSYETVRSV
                     TRQLWDECQTQTPARDQ"
     gene            5884..7425
                     /gene="rny"
                     /locus_tag="PK791_00200"
     CDS             5884..7425
                     /gene="rny"
                     /locus_tag="PK791_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOH91766.1"
                     /translation="MFDLFNDPLNLIWIALALLIGILLGGFIVASVRKNRDIQLKRTA
                     EDIIAAANEEARQVQLEAKDDALKITRQAENELQQRRSELNKEDDRLVKRREELDRRF
                     DQMQQRESSLNKRQSSIDKRANEIDNLYNERLNELQRVAELTTDEARDILLEEVERTA
                     RNDMARIIRQIENEARETGEEKARELIADAIQRVASDKVAEVTTSIVNLPNEEMKGRI
                     VGRNGRNIRSFEQATGVDVIVDDTPEAVTISCFDSVRREIARRTLDRLVQDGRIHPAY
                     IEKILSEERAEMDKDIVKAGNEAAYDASVHGLHPEIIKKLGRLKFRTSYGQNQLAHAV
                     ETSKIASVLAAELGANVEIAKMGALLHDLGKAMDHNTDGTHAQIGAEFAKRHGVSPAV
                     VNIIASHHHEVDQTSIEAVIVEAADAISGARPGARREDLEQYIKRLKALENIAIQHKG
                     VESSFAIQAGREVRIIVKPEEVDDYQAHQLAKNIAEEIEETLQYPGQIRVTVIRETRS
                     VSYAK"
     gene            7480..8169
                     /locus_tag="PK791_00205"
     CDS             7480..8169
                     /locus_tag="PK791_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOH91767.1"
                     /translation="MKTTFTWYGHATLGLTVGEHKLLVDPFFTGNPVAPITADEAFAD
                     FILITHGHGDHVGDAQAIAKRNNALIISNFEIAGWFAEQGLESHAQHLGGGFHHPFGY
                     LKLTLALHGSALPDGGYGGNPAGLLLTTPDGKKIYMAGDTGLFGDMQLIGDEGIDLAV
                     IPIGDNYTMGPDDALRAVKLLRPKQVVPIHYNTWPLIEQDPHAWKARVEAETSAKVVV
                     LKPGESLDLDS"
     gene            complement(8254..9108)
                     /locus_tag="PK791_00210"
     CDS             complement(8254..9108)
                     /locus_tag="PK791_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH91768.1"
                     /translation="MKKINKLRPLALIIVFFLSFTLLGQFAAPSRFASAQDAPFNTAT
                     PDANGYIYHTVRENEYCYTIAELYNTSVERIISLNNLDLDCTIFVGRTLLVAIVTATA
                     VPPTAEPVPAQPTVPPQEAQPTQPPADFVPEPTAMLDTGKVCIILFHDVDGNGMRTDG
                     ENFLYGGEVSINDRTGTVSRVSTTVAGDPDTTKPMCFEQLPPGEYNISIAVPDGFNNT
                     TATNHAITVVSGETITIDFGAQEATPEDMNMQGEARALSPLLFILGGVILLSGLGLLF
                     YMVRARKR"
     gene            9290..9832
                     /locus_tag="PK791_00215"
     CDS             9290..9832
                     /locus_tag="PK791_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOH91769.1"
                     /translation="MEQDNIQNEPSHTLEMLTHIEANPDISQVGLATELGVAVGTINW
                     HLKRMINKGYVKVKRAQRKKLRYIITPEGIALRAALTVDYIKNSFNLYRLVRERTLNT
                     LKELRYQGYEQIRIEGGEDEVAEVARLTCIEQGVSICDKSDSKTPTIYIQGIKLALKL
                     PGETSSDVAPETENRGAHVE"
     gene            9822..10781
                     /locus_tag="PK791_00220"
     CDS             9822..10781
                     /locus_tag="PK791_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOH91770.1"
                     /translation="MWNNLDLSQKRLCVTGGAGFLGKHLIARLEHYGAKDIFVPRSAE
                     YDLVKGDDIARMIDDAQPDIIIHLAAKVGGIGANMEKPGEFFYDNLMMGIQLIHQAWQ
                     RGVEKFVAIGTICAYPKYTPIPFKEEDIWDGYPEETNAPYGLAKKMLLVQSQSYREQY
                     GYNSVFLLPVNLYGPGDNFDPASSHVIPALIRKCLEAKERGDKQIVAWGDGSPTREFI
                     YAEDAAEGIALATMRYNSSDPVNIGSSFEISIKDLTETIARLCDFEGDVVWDTSKPNG
                     QPRRKLDTTRAKERFGFEAKTDFEEGLMKTIAWYKEERACGRG"
     gene            10768..11625
                     /locus_tag="PK791_00225"
     CDS             10768..11625
                     /locus_tag="PK791_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH91771.1"
                     /translation="MAVAENSKTAKSAPNTVVIKARKGLLGIDLKELWQYRELIYFLT
                     WRDIKVRYKQAVLGIAWAILQPVMTTAITSLVFGLLLKVKSDGLPYPVFVLSALLPWH
                     LFQQSLQKSSVSLVGNANLITKIYFPRIIIPISSVLAVMVDFFISLILLFIAMAIYGI
                     TLTWIALWVIPLTLLAVLAALAVGLWLSALNVQYRDVQQMVPFLIQIWMYATPIVYPI
                     NTIPEGIIRYIYSLNPMVGVVQGFRWALFGGSPPDMTLLVSTAAVLVLLAGGLFYFKR
                     MERTFADVV"
     gene            11625..12908
                     /locus_tag="PK791_00230"
     CDS             11625..12908
                     /locus_tag="PK791_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH91772.1"
                     /translation="MTNNNYAVMVKGIGKRYKIGVAQQRQDTLRDVLVAGARKIGNAF
                     RRDKIAKASVDHIWALRDVSFEVKQGSAIGIIGRNGAGKSTLLKILSRVTEPTEGYGE
                     LRGRVGSLLEVGTGFHPELTGRENIFLNGAILGMTRKEISARFDEIVDFSEVEKFIDT
                     PVKRYSSGMYLRLAFAVAAHLEPEILVVDEVLAVGDADFQRKCLGKMGDVAESGRTVL
                     FVSHNMSAILRLTEETIVLDKGRVIKRAPSAEAVDFYLNRGMSKLGERYWEPDEVPAN
                     AAPFRPLAIRVVDKDGVTSDSLRSVDPITIEIDYELEQDITGLRVGFYLNSTRGEAIF
                     TSFDTDSEDLFKEFTMRPKGVYTSRCLIPADTLNEGRFVLGVNASSYMVRRYFQDDQA
                     LSFSVDASGAPGTHWPEPRPGGVRLPLEWKIQEKG"
     gene            12912..>14392
                     /locus_tag="PK791_00235"
     CDS             12912..>14392
                     /locus_tag="PK791_00235"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91773.1"
                     /translation="MKNHIKDFIGMIPYSAELFWAVRGSRRPWSAHYRLGGIKDVLTQ
                     SVHDVQRYAKQNAHPKKVFIFASLHYWIEQAGLIALALAGQGHKVQLSYLPFAEWDKD
                     INRFDVRRQDLYTQDLFKPLRNVIGVIPLLPDLLRYYRWDESEYPAELVKIAKKVSRY
                     DAMYTKQIEEIGEDDELYTFRLRRNLPTALALQRVFEREKPDVVIIPNGTILEMGVAY
                     QVARAMGIETVTFEFADQRERIWIAQNDEIMSHDTTALWQGLGGGDLPDKARQAMVEL
                     FSARKGARLWGDFARQWQQNPSQGASAVREALGLDERPIALLPTNVLGDSLTLGRERI
                     TPTMADMIVGTIKHFIEHPEAQLVIRVHPGELKVHGTSMLDVIRENFKSLPENIHIVK
                     PADKTNTYDLVEIADLGIVFTTTVGMEMAMAGIPVIVTGKTHYANKGFTHEPADWQAY
                     TKLLKEVVADPAAYRLSEKQIEQVWLYAYLFFFEYSLPFPWHLL"
BASE COUNT         3783 a         3696 c         3424 g         3489 t
ORIGIN      
        1 gacgccagag agttttcttg agagcacgct atgtcggggc agaacctcgt cgatgcgccc
       61 agtaccatcc gcctggggtg caaccatcac ctgatcgccc accgctacca aatcaccttc
      121 agcacgccca cgacgcaagc gcccacgcac ccgacatgca tagtcgccct gcgaagtgcg
      181 caccgtcatc cagccagctt gcgaacgaat cactaaacct tgcagcaatt cttgcatctc
      241 gctcaaggtc tattcctctt cttccagggg ctgataaggg cttttttccc agggcattgt
      301 gccaccgggc tcttcagcat cggaataata taaggaaata gcccgcctaa acaaaggggc
      361 tgccatttca gagccttcgc caccatattc cagtatgact gccacagcga tgtctggctt
      421 atcagggtct tcattaaagg tatagccaaa aaaccaggca tggggtttac ctaaagcagt
      481 ttcggcagtc ccagttttac cagcaatatt gccactaaaa cgcgaaaact ggaaatttgc
      541 tgttcctctg ggattgcgaa taacttgaac cataccttct tgcactgctg ccaggttttc
      601 tggcaaagta tcaatatggc gaatgagatc cggttctgtg aattgataaa ctggttcttc
      661 acccataaat ccgatacgct cgataacagt cggacgatgt aaatagccac cattacctat
      721 cgccgcggcg agattagccg cttgcaaagc actcacttgc aaagtgcttt gcccaattgc
      781 catttggaag taatcaaacg cattatcagg gtagttgacc gcaccagcaa attcttcgat
      841 ttcgattcct gtaagcgtgc ccaatccgta ggcaagggct tcatcaggaa tagatgtcgt
      901 atagccatca ttccatagag tgagcccaat gtgccagaac cacgggttgc acgaacgcat
      961 caagccctcc acaagcgtta aatctccatc ggcactaaaa cctctcttgt aagtccagtt
     1021 ttcgcctacc caaccatcat actcagtcca aaggttgtcg catgaataaa cacggtcggg
     1081 agtgaaaacg ccagtttcga gtgcggatat catcgaaata accttaaaaa tcgaaccggg
     1141 cgcgtaaacg ccttgggcag cccggttata caaaggctgt cgcacgttct gaatgagatt
     1201 cgatacttca taatgggtgt gaataagcgg attaaaaatg ttggggtcaa agccagggct
     1261 tgaaaccatt gccaagatct tactggtatc acgctcaagc accacgaccg cgccaaccct
     1321 gtcgcccatt gaccgttgca accaatactg caaacggctg tcgatggtgg ttgtaatgga
     1381 ttgcgctggc tcagcttcag ttgaagccag cttggttaca atcccgcctt ctttgttttt
     1441 cacataaaga tccgcgccac gtttgcccgc gagatacttt tcaccccaac gttcaagacc
     1501 acttgcgcca acagtttcat caattttgta acccaaacgc tgataacgct cctggtcttc
     1561 ggcagagatt cccagcatat agcccgtaac gtgtggggca atgccattat cataataaaa
     1621 acgcgattta aatggctgca agtaaagacc atcaaaacgt tcaattcgcg aactgttttc
     1681 cgcagcaact tcagctgaaa tatcaccgat tactaccgac cagttcgact gagcgtaagc
     1741 atatttgagt cgcactgcat cttcagtttg gttggtcagc tctgctaaaa agctcaccaa
     1801 attgtcttcc tgggccggat taatcttgcc gggtatgatc gttaatgatg ccgcgtcggt
     1861 atatgccacc agcggatagt tatttgaagc atccgatgaa tagatattac cccgggttgg
     1921 aatggtaagc accaactcaa gatagttacc gtccgcaagc tcaggcaaca tcatggaagg
     1981 gtcccagttg attttccatt ccccgccttc aagttgcaaa ttcatcaggg tgtcgcgctc
     2041 caatgtgcca attaggttgg tttcgtagct aatgtgataa ctcacttggg cggcgttggt
     2101 gctgagcaga ttcgacagga tttgaaaatc aactgatttc atggtcaggt cagcagctgt
     2161 tttcttgtgg cgggcaatga agtcctcaag actgtaagca gccttggcag gaatggtcaa
     2221 aaactcatac atcctgttgt agtcttcaat tttccagttt tgaaaatagt ttgctgccaa
     2281 agcttcagca tcgggcagtt cctcccgcga taccgatggg cttggtaaag tcgcagtcac
     2341 ttgtggtgtt ggcgtttcgg tgtttttagt tatcggatca cacgcgctaa gcaataagct
     2401 aaagatgaca aagataagaa taagtcgttt cataagcatt aaccttacac tttctgcaga
     2461 accttctcat gcagaacatc tggatagtaa ttacaacttg tgccagtata agcctggcaa
     2521 gcttcgggca ggttcgcagg aagtggcaaa cccttttctt taaacatgcg ataaagcaaa
     2581 caaagtggta gacccatcac atttgcatag cagccttcaa tgcgctcaac aggatgaaag
     2641 ttgtcatcct ggatggcata accacctgct ttgtcgtgat aggtgccttt atatatgtag
     2701 gcttgctgtt cttcatcgct ccactggcgc atgtgaaccg tggttttgca cacttcgcac
     2761 caggagtccc cgcttgagac ctcaaacagg tttatagcgg tatacacatc atgtggtctg
     2821 ccacgcaatg tgtctaaagt gcgtctggca tcttcatcgt cggcaggctt acccaaaacc
     2881 ttaccgtcaa tcagaacaat cgtatccgcg gagaatatga tttcgcccgg actggaagcc
     2941 acattttggc cctttgccac cgccagggcg cgaacaaaat ccagcgcgga ttgctcaccc
     3001 ttttgcggtt caggataagt cccgggacgc aagtcaaaag gcaagcccaa aagcgccaaa
     3061 agttccctgc gacggggcga agcagatgca agtatcagct ttcttttttc ttccataatc
     3121 ggatttgatt ctaacatgaa tcacaaatgc gaaaatagca gcctatgcca aatggctaat
     3181 cttcgcgcgc tttaatctgg aagatgtcaa aatcagccgc cagatgtgtg ttcgggaaaa
     3241 tcgcctgtgc ttcagccaaa atggcatcat cgcgatagcg acgggaaaca tggttgagaa
     3301 tgagcatctt cgcgttagcc tgtttggcca gctgtgcgct ctgctccgct gtgctgtgac
     3361 caaacttttc tgccatatcc agttcttcag taagataagt agcctcagtt acgatagcat
     3421 ccgagtcgcg cacataatca atcagcttga acggtttacc cacgtcgcca acaacagaca
     3481 gtttcgtgcc cggtcgcgct tcacccaaaa ccatgtcggg actaatcacc cgtccatcct
     3541 ggagtgtaat ttccttgcct tgcacaaggt ccttgcgcca tggaccgggt ggaatatcaa
     3601 gtcgttcagc ggcatctgtc aggaatgggc ggtgcggaaa ttcttcaaac acatagccca
     3661 gggaagaaga accccgatga tccaccggaa aagctctgat ttcaaatttg tttgttctaa
     3721 aaatcggacc gggtctgact tcaattaagt tgagtggcat tccaggttcc tgcccgcgca
     3781 aaacaacgcc ataaagcagg tcatgcacgc ggtccagggt atgttgtgtg ccataaatat
     3841 tgacttcttc aattgcttcc caacgcataa aagtggaaag caaacctgcc aaacccagga
     3901 tatgatccaa atgcccatgc gtgagcagaa tatgattgag acgtttaaaa ccgacaccgg
     3961 agcgcaggat ctggcgctgg gtgccttcgc cacaatctac cataaagcga tactcgtcgt
     4021 gcatcaccac cagcgacgac aaaccacggc gaattgaagg cgccgatgca gaagtgccta
     4081 aaaatacaag ctcaaacaaa gcttaagtcc tgtgattatc cctttttacc ggtcagctgt
     4141 tgcatcacat taaaagcatt cagtccggct ttaagactgg cagagccgga tagtggaaaa
     4201 cgtccttcgt tttcttccat ttctttaacg aggtttaatg ccacttttac accgacaagc
     4261 gctccaatca acgttcccgc gatgatgatt aaggtttttc cggttttgtt catttctgct
     4321 tcccttcagg tgagatgtga gatttcatgc tttcgccaac ttgttttact tttgagccaa
     4381 gttcctggac agtcattacg ggttctaccg aagcatccgc caccgtgcga acgccttctt
     4441 cggcttgtat aacaccaccc aaagcgattt tgccataatt tgggagtgtt tggttcagct
     4501 tatacaagcc atctattgtt ttaaacaaaa tgtagatgag taaaaaccca accaacagtg
     4561 ccagaaacaa gacaataatc aaggtaattt gcccggcttt atagccaacg attccacttg
     4621 agtcactaag cgcggaaacg atggcaaaag ccaagacaat cacgagtaaa acaccaacaa
     4681 cgataatcgg caaaataatc tgcttaaagc gttgtttctt ttggcgttct tgcgcttctg
     4741 tatgtaagcg ttcaactttt aattttattt catcgtccat gccataattc tagcatgata
     4801 tgcctatgat ttcacggttt tcttccttta aaaaattgtc tttttgaatt tcacaaattc
     4861 tggcacattt catgtataat gttattaata aaaatacacg aatgggttct gtatcatagc
     4921 agatgcccat ggcgacgaaa tcgtataacg atttatttgg cagttcagac tgagacaacg
     4981 ctcagttaat gttttggaaa agaaataagc aaatgcttgc gaaaccacga ttgagaataa
     5041 gataccattt aaaagaagga catctggcat cttcttgatt cttggcttgt gcttttactt
     5101 tattgctcct taagggcttt tcggttctaa taagcccaaa ctgccagtaa aaccgcagtt
     5161 attattgata tttcattttc gttgccatgg gcggagaccc atggctgttt gattaatatg
     5221 acgaaagtga ttactgcgat taccgcgcaa aaacgaaata cgcaacggct aaacatcagt
     5281 ctggatggcg aatatgcttt tagccttgac cgcctgactg ccgcatggct taaagtcggt
     5341 cgaaagctca gccccgaaga aattgtttcc ttgcaggaaa aagacgagca ggaagtcgct
     5401 ttcaaccgcg ctttgcgtta tctcagttat cgcgccaggt ctgaagctga aatgcgcaag
     5461 tacctttcag acaaaggctt tagcgaccat gtttctcaaa cagtcataga ccgtctcaaa
     5521 gatgaacgtc tcatcaacga cccccgcttt gcccaggact ggattgacaa ccgcgtgagt
     5581 tttcgtccgc gcagtcaaac ccagttacgc tttgaactgc gcaacaaagg cttgtccgaa
     5641 gatctctcga agatgccctg caagaagctg atttagacga catcgaactc gcctgtgtgg
     5701 cgggcaaaaa gctggtcggc agatatgcca ggcttggctg gcttgatttt cgccaaaagc
     5761 taggcgcggc actggcgcga cgtggtttca gttacgaaac cgtccgcagc gtaacccgac
     5821 aactttggga cgaatgccaa acacagaccc cagctcgtga tcaataatgg agcacagata
     5881 ataatgtttg acctttttaa cgacccacta aatttaatat ggattgccct ggccctgctg
     5941 attggcatcc tgcttggtgg tttcatcgtt gcaagtgtgc gcaaaaaccg cgacattcag
     6001 ctaaagcgaa cggctgaaga tatcatcgcg gcagccaacg aagaagcccg ccaggttcag
     6061 ctcgaagcca aagatgacgc cctaaaaatc acccgccagg ctgaaaacga actgcaacaa
     6121 cgacgcagcg aactcaacaa ggaagatgac cgacttgtaa aacgacgcga agaactcgac
     6181 cgtcgctttg accaaatgca acagcgcgaa agttcgctca acaaacgcca aagcagcatc
     6241 gataaacgcg ccaacgaaat tgacaatttg tacaatgaac gtctaaacga attacagcgt
     6301 gtagctgaac tgacgactga cgaagcccgc gacatcctgc ttgaagaagt cgaacgcact
     6361 gcccgcaacg atatggcacg catcattcgc caaatcgaaa acgaagcccg cgaaacgggc
     6421 gaagaaaaag cgcgcgaact catcgccgac gcaatccaac gcgttgcttc agacaaggtt
     6481 gctgaagtga ccacatcaat cgtaaacctg cccaacgaag agatgaaagg gcgcattgtt
     6541 ggccgcaacg ggcgcaacat ccgctcgttt gaacaagcca cgggtgtgga cgtcatcgtt
     6601 gatgacaccc ccgaggcggt aaccatctcc tgttttgact cggtccgccg cgaaatcgcg
     6661 cgacgtacac ttgaccgcct ggtgcaagat ggcagaatcc atcccgccta cattgagaaa
     6721 atcctgtcag aagaacgcgc tgaaatggac aaagacatcg tcaaagcggg taacgaagcg
     6781 gcctatgacg catctgttca tggcctgcac ccggagatca tcaaaaagct tggccgtctt
     6841 aaattccgca cgtcctatgg acaaaaccaa ctggcacatg ctgttgaaac cagcaaaatc
     6901 gccagcgtgc tggccgctga actcggtgcc aacgttgaaa tcgccaaaat gggcgcgctt
     6961 ctgcacgacc tgggcaaagc catggaccac aacaccgatg gcacccacgc ccaaatcggc
     7021 gcggagtttg ccaaacgcca tggcgtaagc ccagcggttg tcaacatcat tgcctcgcat
     7081 caccatgagg tagaccaaac atccattgaa gctgttatcg tagaagctgc tgatgctatc
     7141 tcaggcgccc gccccggtgc ccgacgtgaa gaccttgaac aatatatcaa gcgtcttaaa
     7201 gcgcttgaaa acattgctat tcagcacaaa ggcgttgaat ccagtttcgc cattcaagcc
     7261 ggtcgcgaag tgcgcatcat cgtcaagccc gaagaagttg atgactatca agcccatcaa
     7321 ctggcaaaga atatcgccga ggaaattgaa gaaaccttac aatatcctgg tcaaatccgc
     7381 gtaaccgtca tccgcgaaac gcgctcggtc agttacgcca aatagaacaa ttcaaaagcc
     7441 cccctgtggg gctttttatt atcatcaaac ggaggttaca tgaaaacaac atttacctgg
     7501 tacggacacg ccacacttgg cttgacagtt ggcgaacaca aacttttggt tgaccccttc
     7561 ttcactggca acccggtcgc cccgataact gctgatgaag cctttgcgga cttcattctc
     7621 atcacccacg gacacggtga ccacgttggc gacgcacaag ccattgccaa acgcaacaat
     7681 gcgctgatta tcagcaactt tgaaattgcc ggatggtttg ccgaacaagg gctggaatcc
     7741 cacgctcaac acctgggcgg tggttttcat cacccctttg gctatctcaa gctcactttg
     7801 gcactgcatg gctccgcttt gcctgatggt ggctacggtg gcaacccggc tggcctgctt
     7861 ttgaccacac cggatggcaa gaagatttac atggcgggtg ataccggtct gtttggcgat
     7921 atgcaactca tcggtgatga aggaatcgac ctggcagtca tccccatcgg cgataactac
     7981 accatgggcc ccgatgacgc cctgcgcgcc gtcaaacttt tgcgacccaa acaagttgtg
     8041 cccattcact acaatacctg gccactcatc gaacaagacc cccacgcctg gaaagcccgt
     8101 gtggaagctg aaacaagcgc caaagttgtg gtactcaagc ccggcgagag tcttgactta
     8161 gacagttaag cttctttaga aaggctaaac ctttaaaacg aaaaggagac gcaacatgta
     8221 cgtctccttt tttgtttcct gatattcagg gatttatctt ttccttgccc taaccatata
     8281 gaacaacaag cccaaacccg aaagcaagat gacaccaccc agtataaaca gcaatggaga
     8341 cagagcgcgc gcttcacctt gcatgttcat gtcctctggc gtggcttcct gcgcgccaaa
     8401 atcaatggtt atggtttctc cggaaacgac tgtaatagcg tgattcgtgg ctgtcgtatt
     8461 gttgaaccca tccggcaccg cgattgagat attgtactca cctggcggga gttgttcaaa
     8521 gcacatcggc tttgtggtgt cagggtcacc tgccaccgtc gtgctcacac gcgagaccgt
     8581 gccggtgcgg tcattaatgc tcacttcacc accatataaa aagttttcgc catcggtacg
     8641 cataccattg ccatctacat catggaaaag aataatgcat accttgccgg tatccaacat
     8701 cgcggttggc tctggaacaa aatccgccgg tggctgggta ggttgcgctt cttgcggggg
     8761 aacagttggt tgggcgggca caggttctgc agtcggggga accgcggttg ctgtaactat
     8821 tgctaccaga agcgttcgtc cgacaaaaat cgtgcaatct aaatctaaat tattcaaact
     8881 gatgattcgc tcaacagaag tattataaag ttcagcgatg gtatagcaat actcattttc
     8941 cctgaccgta tggtagatgt aaccatttgc gtccggtgtc gctgtattaa aaggtgcgtc
     9001 ctgcgcgctg gcaaagcgac tcggcgcagc aaactgtcct aaaagggtaa aggacaaaaa
     9061 gaatacaatg atcaatgcca aaggcctaag tttgtttatc tttttcatag caaactcctt
     9121 tgcttagatt atatcattga ctgaaaagtc cataatcgcg cgataccaag atcttaggat
     9181 aattataatt cttttctgga gagatgcatt ttcgcattcc agtcatacca tcgcgcgcgc
     9241 agagttgacc ccgcagcaaa tgggttttat aattcagtca ttgaattata tggaacaaga
     9301 caatattcaa aacgaaccca gccacacgct ggagatgctc acgcacattg aagccaatcc
     9361 ggatatctcg caagtcggtt tagcgaccga acttggcgtg gcggtgggca ctatcaactg
     9421 gcatcttaag cgcatgatta acaagggcta cgtgaaagtc aagcgcgccc aacgtaaaaa
     9481 gctgcgctat atcatcacgc ccgaaggcat cgctctgcgc gctgccttaa ctgtggacta
     9541 tatcaaaaac tcctttaacc tttaccgcct ggtgcgtgaa cgcacgctca atacattaaa
     9601 ggaattgcgc tatcagggct atgagcagat tcgcattgaa ggtggcgaag atgaagtggc
     9661 agaagtggcg cgtttgactt gtattgaaca aggtgtaagc atttgcgaca aatcagacag
     9721 caaaacgccc actatctata ttcaaggcat caaacttgca ttaaaattac ctggcgaaac
     9781 gtcatctgat gtcgcgcctg aaacagaaaa cagaggtgct catgtggaat aatttagatc
     9841 tcagtcaaaa acgcctttgc gtcacaggtg gcgcgggctt tttgggaaaa catttaatcg
     9901 cgcgtcttga acattatggc gcgaaagata tctttgtgcc acgctcggca gagtatgacc
     9961 tggtaaaagg ggatgatatc gcccgcatga ttgacgatgc ccagcccgac attatcattc
    10021 acctggcagc caaggtcggg ggcatcggtg ccaacatgga aaagcccggc gagttctttt
    10081 atgacaactt aatgatgggc attcagctca ttcaccaggc ctggcagcgt ggtgtggaaa
    10141 agttcgtggc aatcggcaca atttgcgcct accccaaata cacgcccatt ccttttaaag
    10201 aagaagatat ctgggatggc tatcccgaag aaaccaacgc gccctatgga ctggcaaaga
    10261 aaatgctttt ggtacagtcg caaagttatc gcgagcaata tggctacaac tccgtattcc
    10321 tgttacccgt caacctttac ggacctggcg ataattttga ccctgccagt tcgcacgtta
    10381 ttcccgcgct gattcgcaaa tgcctggaag ccaaagaacg tggcgacaag caaatcgtgg
    10441 cctggggcga tggctcgcca acgcgtgagt tcatctatgc cgaagacgcc gccgaaggta
    10501 tcgcgctggc aaccatgcgc tataacagct ctgacccagt caacatcggc tcgagttttg
    10561 aaatcagcat caaagatttg actgaaacca tcgctcgatt gtgcgatttt gaaggcgatg
    10621 tggtttggga caccagcaag cccaacggtc agccccgtcg caaactggac actacccgtg
    10681 ccaaagagcg ctttggtttt gaagccaaaa ccgattttga agaaggctta atgaaaacaa
    10741 tcgcctggta taaagaggag cgtgcctgtg gccgtggctg agaacagcaa aacagcaaaa
    10801 agcgcaccaa atacagtcgt catcaaagcc cgcaaaggct tgctgggtat tgaccttaaa
    10861 gaactctggc aataccgcga actgatttac tttttaacct ggcgcgatat caaagtacgt
    10921 tataaacaag cggttttggg catcgcctgg gcgattttgc aaccggttat gaccacagcg
    10981 attacctcgc tggtgtttgg cttgttgctc aaagtgaaat cggatgggtt gccctatccg
    11041 gtgtttgtgc tctcggcgct tttgccctgg catcttttcc agcaaagtct gcaaaaaagc
    11101 agcgtgagcc tggtaggaaa cgccaactta atcaccaaaa tctacttccc gcgtatcatc
    11161 attcccatca gttcggtcct ggcggtgatg gtggactttt tcatctcgct tatcttgctc
    11221 tttatcgcca tggcgattta tggcatcacc ctgacttgga tcgccctgtg ggttatcccg
    11281 ctcacgcttt tggctgtttt ggccgctttg gcggtgggac tttggctctc ggcactgaac
    11341 gtgcagtacc gggatgtgca acaaatggtg cctttcctta ttcaaatatg gatgtatgct
    11401 acccccatcg tttaccccat caacaccatt ccagaaggga taattcgtta tatctacagt
    11461 cttaacccga tggtcggtgt cgtgcagggc ttcagatggg cgctttttgg tggttcaccc
    11521 ccggatatga cgcttttggt ctccaccgca gccgttttgg ttttgctggc aggcggactg
    11581 ttttatttta aacgtatgga aagaactttc gcggatgtgg tgtgatgacg aacaataatt
    11641 acgcagtaat ggtcaaaggt attggcaaac gctataaaat cggcgttgcc cagcaacgtc
    11701 aggacacctt gcgcgacgta ctggtcgcag gcgcgcgaaa aatcggcaat gctttcaggc
    11761 gcgacaaaat cgccaaggcg tctgttgacc acatttgggc tttgcgcgac gtcagttttg
    11821 aagtcaaaca agggtcagcg attggtatca tcggccgcaa tggtgccggc aaaagcactt
    11881 tactgaagat tctctcgcgc gttaccgaac ccaccgaagg ctatggcgaa ctgcgcggtc
    11941 gggtagggtc tcttttggaa gttggcacag gttttcatcc ggaattgacc ggacgggaga
    12001 acattttcct gaacggcgcc attttaggca tgacccgcaa agaaatcagc gcccgttttg
    12061 acgaaatcgt tgatttttct gaagtggaaa agtttattga cacacccgtg aaacgctatt
    12121 cttcgggtat gtatttgcgc ctggcattcg cggtcgcagc gcatctggaa ccggaaattt
    12181 tggttgtgga cgaagttttg gcggttggcg atgccgattt tcagcgcaaa tgcctgggca
    12241 aaatgggcga cgtggccgaa agtggccgaa ccgtgctttt tgtcagccat aatatgtccg
    12301 ccattttgcg cctgaccgaa gaaaccatcg tattggacaa ggggcgtgtc atcaagcgcg
    12361 cgcccagtgc cgaagcggtt gatttttatc tcaaccgggg catgtccaaa ctgggtgaac
    12421 gctactggga acccgatgaa gtccccgcca acgccgcgcc cttcagaccg cttgccatcc
    12481 gcgttgtgga taaagacggt gtaacttcgg acagcctgcg cagtgttgac ccgataacga
    12541 ttgaaattga ctatgaactt gaacaggata tcaccggtct gcgggtgggt ttttatctca
    12601 acagtacccg tggcgaggcg atatttacca gttttgatac ggattcagag gatttattca
    12661 aggaattcac catgcgtcct aaaggtgttt acaccagtcg ctgccttatc ccggccgaca
    12721 cactcaacga agggcgtttt gtcctgggtg tgaacgccag ttcttatatg gtacgacgat
    12781 acttccagga cgaccaggca ttgagttttt cagtcgatgc cagtggcgcg ccgggcaccc
    12841 attggcccga accccgccca ggtggtgtgc gcctgcccct tgaatggaaa atccaggaga
    12901 aaggataatt gatgaaaaat catataaaag actttattgg gatgataccc tatagcgcgg
    12961 aactcttttg ggcggtgcgt ggttcgcgtc gtccctggtc agcgcactat cgattgggtg
    13021 gcatcaaaga tgtactcacg caatctgtgc acgacgttca gcgctacgcc aaacaaaatg
    13081 cccatcctaa aaaagtcttt atttttgcgt ctttgcatta ttggattgaa caagccggac
    13141 ttatcgcgct tgccctggca ggtcaggggc acaaggtgca gttgtcctat ttgcccttcg
    13201 ctgagtggga taaggacatc aaccgctttg acgtgcgccg gcaggacctt tacacacagg
    13261 acttattcaa acctttacgc aacgtcatcg gcgtgattcc gcttttgcca gaccttttgc
    13321 gctattatcg ctgggatgaa agcgaatatc ccgctgagct ggtgaaaatc gctaaaaaag
    13381 tctcccgcta tgacgccatg tacaccaagc agattgaaga aatcggcgaa gatgatgagc
    13441 tttatacctt tagattacga cgcaatttgc caaccgcctt ggcattgcaa cgtgtctttg
    13501 agcgtgaaaa gccagatgtg gtaattatcc ccaacggaac cattttggag atgggcgttg
    13561 cctatcaggt cgcccgcgcc atgggtattg aaacggtaac gtttgagttt gccgaccagc
    13621 gcgaacgcat ttggatcgcg caaaacgatg aaatcatgag ccacgataca accgctttgt
    13681 ggcaaggtct gggcggaggc gacttgcccg ataaagcccg ccaggcgatg gtggaactgt
    13741 tttccgcgcg aaaaggcgcg cgcctttggg gcgactttgc ccgacaatgg caacaaaacc
    13801 cctctcaggg cgcttccgcg gtaagagaag ccctgggttt ggacgaacgc cccatcgctc
    13861 tcttgcccac caacgtcctg ggcgacagcc tcacgctggg tcgcgaacgc atcacgccca
    13921 ccatggcgga tatgattgtg ggcacgataa aacactttat cgaacatccc gaagcgcaac
    13981 tcgtcatacg cgtgcatccc ggcgaactaa aagtacacgg cacatccatg ctggacgtca
    14041 tccgtgaaaa cttcaagtct ctgccggaaa acattcacat cgttaaaccc gccgataaaa
    14101 ccaacaccta tgacctggtc gaaatcgccg atttaggcat cgtctttaca acgaccgtgg
    14161 gcatggagat ggcgatggcg ggcattcccg tgattgtgac cggcaagacc cactatgcca
    14221 acaagggctt cacacatgag cccgctgact ggcaagccta taccaaactg ttgaaagaag
    14281 tcgtggcgga tcctgccgcc taccgcctta gcgaaaagca aatcgaacag gtttggctgt
    14341 atgcctatct cttcttcttt gaatactcgc tgcctttccc ctggcatttg ct
//
LOCUS       DAOSFC010000004        12626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_4176_length_12626_cov_5.085435, whole genome shotgun sequence.
ACCESSION   DAOSFC010000004 DAOSFC010000000
VERSION     DAOSFC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12626
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4176_length_12626_cov_5.085435"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PK791_00240"
     CDS             <1..592
                     /locus_tag="PK791_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91774.1"
                     /translation="KIRKMSVEALTEDILTHLDNAEEHLAEIFGMADWMNVMHKNRVI
                     ADTHETSRKGLVKELGEAQYEAIAHLPLEEIPEDLHERIQGVLGYETQNRVYRDLLLG
                     TFTEQWVEYLTEMEALRVSIRMESYAQQDPLVQYKLKASGMYSELMGTVRQIVVSRMF
                     RFRPNLASTQDSGTQTASGNDSESGGKRKKKRRRRR"
     gene            622..1398
                     /gene="surE"
                     /locus_tag="PK791_00245"
     CDS             622..1398
                     /gene="surE"
                     /locus_tag="PK791_00245"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOH91775.1"
                     /translation="MKKILLTNDDGVRAPGILAMAQALRKADFDVTILGPDTNWSASG
                     HKKTMNKPLRISKVQLADGSWAFATDGAPSDCVALGLMGALDMEFDFIVSGINNNANI
                     GSDVSYSGTVAAVLEAGIMGKPGFAVSVDAPEYNDGNLDYLSTASKAMEIIIKLLEMP
                     MEDQDMNVWNINLPYFPDGSYKGVQFTRLGKRYYHDFLLRREDPFGRPYFWIGGEQPT
                     GLMEDHTDYGALKTGFVSVTPLHIDSTNYAVLEKLEKNLD"
     gene            1420..2976
                     /locus_tag="PK791_00250"
     CDS             1420..2976
                     /locus_tag="PK791_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH91776.1"
                     /translation="MLPDFRVRQRDYLLEISRALTEELNLPVLLTRILRITVEMLSGQ
                     AGFIALLDENQGWQIAVHQGLPQALFTYIQDWMANLPDDIESDRERAFEMNKMLNDVS
                     MGMLTSVGISMVAQGEVIGQIYVFRLHQASFTQNDYSLLNSFANQAAIAVRNAKLYKE
                     ISESNLRTVALLDSVADGIVILGPDLRVLRVNSAFCKLIHASPKTLLDRPSDEVIQLQ
                     GKLKGIPIEQVLAHDWKRYTREEMFIEGDMLRQGGLAPLPVSINYAPLFTKDGELLNV
                     IASVRDISRHRAAEDMKTSFMSVISHELKTPIALIKGYASTLRRDDVEWDKAMIEESL
                     RVIEEESDHLAAMVEDLLDATRLQAGGLALRPTELDIDALVEDLLRKHTVQAQDQPLI
                     THLPHDFPLVYADPTRINQLLRNLISNSLKYAEEGPIEVDGQAFDDYVQICISDHGPG
                     FDPMDVPHAFDRFYRADHSSKTSKGTGLGLFLSKSIVEAHGGKIWIDENYHKGAKICF
                     TLPRFFPKSV"
     gene            2996..3778
                     /locus_tag="PK791_00255"
     CDS             2996..3778
                     /locus_tag="PK791_00255"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOH91777.1"
                     /translation="MELQILENIKPVEMAGFKVYTLAETASTNTDVLNLARAGAPDST
                     VVYAKKQTQGRGRMNRVWESKPEQSLAFTILLRPSKDEQAFLFRFTALAALALADVLE
                     ENYGLAAKLKWPNDVLLNGAKVCGILTETLWQGTCAEALAIGMGVNLGKQAYESAKPL
                     RYQAISLEEVTGQLVPPLVLLEAILKRVSQLRPQIGSIEFIQKWNEKLAFKGEKVKTI
                     NEKGETESFRLIGIQDDAALLLEDAHGKLRQLYSSEISLSSL"
     gene            complement(3745..6393)
                     /gene="gyrA"
                     /locus_tag="PK791_00260"
     CDS             complement(3745..6393)
                     /gene="gyrA"
                     /locus_tag="PK791_00260"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOH91778.1"
                     /translation="MTEKPDNLEQENVPEENENNAEANHTNRGDVELVGIDEQMRTAY
                     LDYAMSVIVARALPDVRDGLKPVHRRILYAMDDMGIRSNTPFKKSARIVGEVLGKYHP
                     HGDSAVYDTMARMAQDFSMRYTLVQGQGNFGSIDGDPPAAMRYTEARLDKIADELIAD
                     LDKDTVDFVDNFDASLKEPEVLPARIPNLLMNGSSGIAVGMATNIPPHNLGELVSALN
                     LLIDRWDDIEEVSVEELMQHVPGPDFPTGGVIVGTEGIRQAYSTGRGHLTMRGEAVIE
                     EIREGRYAIVINEIPYQLNKTSLIERIAELVREGRLDAISDMRDESDRKGMRIVVELK
                     RGTQPRKVLNQLFKYTPLQSTFAVQMLALLDGEPRMFTLKRALYHYLEHRQVVITRRT
                     LYELDRAKKRAHILDGLLIAIANLDAVIKTIRESADADTAKTNLIERFTLSPEQAQAI
                     LDMQLRRLAALERLKIEEEYKQVMETIADLEDILANPQRILDIIRADLNDVATKYSDP
                     RRTRIAPDHEMDLSDEALIQDEPVFVSITNRGYIKRVADSAYRIQGRGGRGVIGQSIR
                     GEDEVVLFERANTLGTMLFFTDLGKIYTTRVFELPEESRQGRGIPLVNLLDLEPDETV
                     TAMLAINSFDEAKYFVMATKLGRIKRLPLHNFANVRPSGLIAINLDQGDTCGWVRKTN
                     GDDDIILATREGKALRFNESQVRVTGRMTMGVMGIRLRGDDEVIGMEVVDEDQTLLIV
                     TQRGQGKRSAMDQFVPKSRGTLGVTCFTRLEDENIGPLAEICMVHEDDEITLISQAGI
                     VLRTKVDQISIQGRATQGVRVMELDEGDTVAALTRISADEYFPHLQSGNAKNPQGDQP
                     ATEPNQPASEVEANADHKEESEISEE"
     gene            6720..7613
                     /locus_tag="PK791_00265"
     CDS             6720..7613
                     /locus_tag="PK791_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOH91779.1"
                     /translation="MLKLRLMTLNLGGGIKNYRGTPQESFDKSEAISELINRIKPDVL
                     GVQEIAQYLDGDGHLHSMTERIRDDAQFDHAFYGETLSMKQHMQVKQALIVEGIFNDW
                     WDWSKGNALFSHLPFARLGDATSSGMPRNVPVFQPARYEGTRDTDPRYVIISRLKASP
                     HPYILNLHLTTLVGERGENAWSSTIEAAEMARSKQILNVLGLIEKHVLIPGEPVILMG
                     DFNAKPDEYTLKNHLVEDNHFVRLVPENDIFTHGSAGTVDHIYFAPAHRLRSYQCWVV
                     DDELTHRVSDHLPVVADIEIE"
     gene            7626..9098
                     /locus_tag="PK791_00270"
     CDS             7626..9098
                     /locus_tag="PK791_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91780.1"
                     /translation="MQTVKYFGTDGIRGTYGNYPMTEEFMTRLGYAVGTVLGHDADAI
                     NEQPIVIIGRDTRQSGPGLQQALTRGLTATGAIVWDLGILPTPGVAFLTQEHQATAGV
                     VISASHNPAEQNGIKFFSRQGTKLSLEQELEIESILDATALENVPAMDESKVFDRLDL
                     QADYLRDLLESQHELNLNGKRILVDCSNGAAYHLAPRALEVLGADVVPINIDSSGDTI
                     NKNAGSERARSKPAELSAIMQEKGADLAIAFDGDADRVIFLDELGRLIDGDHMLAILA
                     DYLHAKDLLLGDTLVTTVMANGALKTYAQNQGFKLKTTPVGDKYVTEALQQILDDTRE
                     LGKIGLGGEQSGHIILLDQTHQTGDGLRTMTFVLAVVMHSDEQSLAKLADRIEKYPQL
                     VASCDVAQKIPLAELKALSARLETLSDDLPGLVEMNSRYSGTENKYRLMLETDTRHTA
                     HDLGKIAWEICALVQAETGTPAGSKIEVLNVADGGLIPKP"
     gene            9127..11670
                     /locus_tag="PK791_00275"
     CDS             9127..11670
                     /locus_tag="PK791_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91781.1"
                     /translation="MFNDKPFVTQLPYRFNRLNRAILVADKPELWQAAIAEMSAFLDE
                     LEAWLHANVNLLGEDLATSSRILSLLLTLAATGSQWRLELFNAKNAYEYKFRKQLDQD
                     YLPKNGAMRRKAIDLAKFYFTAPVFASLQEDIAFEILPLLESLDAKQDPDRLMAYRVI
                     QIGNIYERLYAMRLRTADPLLIGLAGVKGLLREIYNRKYLRFGTSGVRGRWGNDFTEP
                     RARQVLQAICDFMNNANVPAFVGAEDLSGRRVVLGHDTRANADLVTQWAAEVCLANGF
                     RVEMGTRDVPTPALSYYETDVLPQDDVAGLIIATASHNPPEWQGIKFNPRLGYPAPTN
                     VTDFIAFRINELQLVDAAGGQADVQAAKARGLVQGFDPLDSYVRWIKNNGQGNARIPI
                     DFDRIRRYFADKMVVVDEMHGAGRDYLTRLIGEAGVRFTVIHAEVDPDLGGQDYANPE
                     EPFNQLLKDTVVKTGAHLGMGMDTDADRFGIVDRDGTYFRPNQILPMLVRYLGVDRGL
                     TGRIIATQTGSPLLEPLAGMIPGNEANWPVEGALPGYVNQVAYKCQVGDIHTRALRYA
                     FAVPVGIKYIEEIRRTDRAYNALKELPDNWRDTILIGGEESSGLTTRGHVLDKDGPWA
                     NILIMDMVAYYGTREKKPLHSLKEIWDELVSLPGLWESFGTSTDPSSHAGRADVDAPL
                     EAKEGFINYYLDLAQNTGVKNPKIAGLTVDYLGGIRYELVEMQLIDDDGDDHHYLRMR
                     ASGTEPINRIYIESSQLETGQKMMKEVLKRLELITMDVLAKAHSPWHLVDMLTQTTMT
                     ADTLALVKQLIGERGWDKQDILAKIQQMLQTLEKRNRKVITTWYEALQK"
     gene            11836..>12626
                     /locus_tag="PK791_00280"
     CDS             11836..>12626
                     /locus_tag="PK791_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH91782.1"
                     /translation="MKIVCDSGADLTLEEYKELDITVFPLKVEVDGVSYQAGVDISPT
                     EFYDLMDNAENMPKTATPSLGDIVDVYKRLAQEDRDIISVHISSGLSGTSNVARQAAL
                     LVPEANITVVDTLTLSAGQAWQVRAAALMAKANKNREEILEKLGQIQQALTSHFTLPD
                     LKYLIAGGRIGHLKGLLASLLGIKPIIEVNKEDGKYYDVGKRRSFQKAIDNIPELLLK
                     YHQAGAQLRAQIVTAANSEGGEKLKQAMEKVFNVSWEPEVVLGTAL"
BASE COUNT         3136 a         3091 c         3340 g         3059 t
ORIGIN      
        1 aaaaatcagg aagatgagcg tggaagcgct aacggaagat attctcacgc atctcgataa
       61 cgctgaagaa catctggcag aaatctttgg catggcggac tggatgaatg tcatgcacaa
      121 aaaccgcgtg atcgcggaca cgcacgaaac ttcgcgcaaa ggcctggtaa aagaactggg
      181 cgaagcgcaa tatgaagcca ttgcccatct gcccctggaa gaaatccccg aagacctgca
      241 tgaaagaatt cagggcgttc tcggctacga aacccaaaac cgcgtttacc gtgatttatt
      301 gctgggcact ttcactgaac aatgggtaga atatctgaca gagatggaag ctttgcgcgt
      361 gtcgattcgc atggaatcct atgcccagca ggacccactc gtacagtaca aactcaaagc
      421 cagtggtatg tacagtgagt tgatgggcac cgtgcgccaa attgttgtca gccgtatgtt
      481 ccgctttaga ccgaacctgg cttccacaca agatagtggc acccaaaccg cgtcaggaaa
      541 tgactctgaa tcgggtggca agcgcaagaa aaaacgacga agacgccgct aagcgcgtct
      601 gtaaaaggac ttggagactt aatgaaaaaa atcttattga ctaatgatga tggcgttcgc
      661 gcgccaggca tattagccat ggcacaagcc ctgcggaaag cagattttga tgtgacaatt
      721 ttaggacccg acacaaactg gtcggcatct ggtcacaaaa agacgatgaa caaacccttg
      781 cgcatcagca aagtgcagtt ggctgatggc agctgggcgt tcgctacgga cggcgcgccg
      841 agtgactgtg tggcactggg attgatgggc gcgcttgata tggaatttga ttttatcgtt
      901 tctggtatca acaataatgc caacatcggt tcagatgtga gttattcggg cacggttgcc
      961 gccgtgcttg aagccggcat tatgggcaaa cctggttttg cggtctcggt tgacgcgccc
     1021 gaatacaacg atggcaacct ggattatctc agcactgcca gcaaagcgat ggaaattatt
     1081 attaagctgc tcgaaatgcc catggaagac caggatatga atgtctggaa tatcaacctg
     1141 ccttattttc ctgatggcag ttataaaggc gtgcagttca cacgcctggg caaacgttat
     1201 taccatgatt ttctgcttag acgcgaagac ccctttggca gaccttattt ttggattggt
     1261 ggcgaacagc cgactggatt gatggaagac cacaccgatt atggcgcgct gaagacgggc
     1321 tttgtttcgg taacgccatt acatatcgac agtaccaact acgcggtgct ggaaaaactg
     1381 gaaaagaatt tggactaagc gatgagtttg agagtcaaga tgctgcctga ttttcgtgtc
     1441 cgccaacggg attacctcct ggaaatttct cgcgcgctaa cggaagagct aaatttgcct
     1501 gttctgctta cccgcatttt gcgcattacc gtggaaatgt tgagtggcca ggcgggcttt
     1561 atcgctttgc tggacgaaaa tcagggctgg cagattgccg tgcatcaggg cttgcctcag
     1621 gcgcttttca cttatatcca ggactggatg gcaaacttgc ccgacgatat cgaaagtgac
     1681 cgtgagcgcg cgtttgagat gaacaaaatg ctgaacgacg tcagcatggg catgttgacg
     1741 agtgtgggta tttcgatggt cgcgcaaggc gaggtaatcg ggcagattta tgtctttcgg
     1801 cttcaccagg cctcttttac ccaaaacgat tattcccttt tgaatagttt tgcaaaccag
     1861 gctgctattg cggtgcgcaa tgccaaactt tacaaagaaa tcagcgagag taatttgcgc
     1921 acggtcgcct tgctggattc ggtggcagat ggcattgtga ttcttgggcc agatttacgc
     1981 gttttgcggg ttaattcggc tttttgcaaa ttgattcatg cctcaccaaa gactttgctt
     2041 gaccgcccga gcgatgaagt tattcagttg cagggcaaat taaaaggcat acccattgag
     2101 caggtcttgg cgcatgactg gaaacgttat acccgcgaag aaatgttcat tgaaggcgat
     2161 atgcttcgcc agggtggttt ggcacccttg cccgtttcaa tcaactacgc gccactcttt
     2221 accaaggacg gtgaactgct taatgtcatc gcgtcggtac gtgatatcag ccgccatcgc
     2281 gccgctgaag atatgaaaac aagctttatg tcggtgatta gccacgaact caagacgccc
     2341 atcgcgctga ttaagggcta tgccagcact ttgcgtcgtg atgatgtgga gtgggataaa
     2401 gccatgatcg aagaaagtct gcgcgtgatt gaggaagaat ctgaccacct tgccgcgatg
     2461 gtggaagatt tgcttgacgc gacccgcttg caggcgggtg gactggcact gcgccccacc
     2521 gagttggaca ttgacgccct ggtggaagat ttgctgcgca agcatacggt ccaggctcaa
     2581 gaccagcccc tgataacgca tttgccccat gactttccct tggtctatgc tgacccaacc
     2641 cggattaatc aactgctgcg aaacctaatt tcaaacagcc ttaaatatgc tgaagaaggg
     2701 ccgattgagg ttgacggaca agcctttgat gattatgtgc agatttgcat ttcagaccat
     2761 ggtcctggct ttgatcctat ggacgtgccc cacgcttttg accggttcta tcgcgcggat
     2821 cacagcagca agaccagcaa aggcacaggt ttgggtttgt ttttgagcaa atccatcgtt
     2881 gaagcgcatg gtggaaagat ttggattgac gaaaattacc acaaaggcgc aaaaatatgc
     2941 tttactttac cccgtttttt tcctaaatcc gtataatctc acgtgagagt aaaatatgga
     3001 attacaaata ctggaaaata ttaaaccagt tgaaatggct ggttttaagg tttacacact
     3061 agcagaaacc gcatcaacca acacagatgt tctcaatttg gccagagcag gcgcgcccga
     3121 tagcactgta gtttacgcca aaaaacaaac ccagggcaga ggcagaatga atcgggtttg
     3181 ggaatcaaaa ccggagcagt ccctggcatt taccattctc ttaagaccca gtaaagatga
     3241 acaagctttc ctgtttcgtt ttactgccct ggcggctttg gcacttgcag atgttcttga
     3301 agagaattat gggttggctg ccaagctgaa gtggccaaac gatgtccttt tgaatggtgc
     3361 caaagtctgc ggaatcctga ccgaaacgct gtggcaaggc acctgcgcgg aagcgcttgc
     3421 cataggcatg ggtgtgaatt tgggcaagca ggcttatgaa agtgccaagc ccttgcgcta
     3481 ccaggcgata agtcttgagg aagtgaccgg tcagcttgtg ccacccctgg ttttgcttga
     3541 agcgattcta aagcgcgtct ctcagttgcg cccacagata gggtcaatcg agtttatcca
     3601 aaaatggaat gaaaaacttg ccttcaaggg cgaaaaggtg aaaacgatca atgaaaaagg
     3661 cgaaacagaa tcatttcgcc ttatcggtat ccaggatgat gccgcgctgt tgcttgaaga
     3721 cgcgcacggc aagctgcgcc agctttattc ttccgaaatc tcgctttctt ctttgtgatc
     3781 agcgtttgct tcaacttcac ttgctggttg atttggctct gtagcaggct ggtcaccttg
     3841 cgggtttttc gcgttcccgc tttgcaaatg cgggaaatat tcgtccgctg aaatgcgtgt
     3901 tagcgcggcc accgtgtcgc cttcgtccag ctccataacc cgcacacctt gtgtggcacg
     3961 tccctgaatg gaaatctggt caacctttgt acgtaaaacg atgcctgcct gagaaatcag
     4021 cgtaatttca tcatcttcat gcaccatgca tatttccgcg agtggcccga tattttcatc
     4081 ttcaaggcgg gtaaagcagg taacacccag cgtgccacgt gacttgggca cgaactggtc
     4141 catagcgctg cgttttccct gaccgcgttg ggtgacgatg agcagggttt gatcttcatc
     4201 aacaacttcc atgccgatta cttcgtcatc accgcgcagg cggatgccca tcacgcccat
     4261 ggtcatgcgt ccggtaacac gcacctggct ttcgttaaag cgcaaggctt tgccttcgcg
     4321 ggtggcaagg atgatgtcgt catcgccgtt ggtcttgcgc acccagccac aagtgtcgcc
     4381 ctggtcgaga ttgatggcaa tcaaaccact ggggcgcacg ttggcgaaat tgtgcagggg
     4441 caggcgtttg atacgtccaa gctttgtcgc catcacgaag tacttcgctt cgtcaaaact
     4501 attaatagcc agcatagcgg ttaccgtttc atcgggttca aggtccagca ggttgaccag
     4561 gggaatgcct cggccctggc gggattcttc gggaagttca aacacgcgcg tggtgtaaat
     4621 tttgcccagg tcagtaaaga aaagcattgt gcccagtgtg ttggcacgtt caaagagaac
     4681 cacttcgtcc tcgccacgga tgctttgccc gatgactccc cgtccaccgc gaccctgaat
     4741 gcggtaggcg ctgtcggcga cgcgcttgat atagccacgg ttggtaatgg agacaaagac
     4801 cggttcatcc tgaatgagcg cttcgtctga aagatccatt tcgtggtcgg gcgcgatgcg
     4861 tgtgcgacgc gggtcgctgt atttggttgc cacgtcattc aaatcagcgc ggataatatc
     4921 caaaattcgc tggggattag caaggatatc ttcaaggtca gcgatggttt ccatcacttg
     4981 cttgtattct tcttcaatct tgagacgttc tagggctgcc aaacggcgca gttgcatatc
     5041 caggatagcc tgagcctgtt caggactgag cgtaaagcgt tcaataaggt tggttttcgc
     5101 ggtgtccgcg tcagctgatt cgcgaatggt tttgatgacc gcgtcgagat tagcaatagc
     5161 aatcagcaaa ccgtccagaa tatgcgcgcg ttttttggca cggtccagtt cataaagtgt
     5221 gcgacgggtg ataaccacct ggcgatgttc caaatagtgg tagagcgcgc gcttgagcgt
     5281 gaacatgcgt ggttcgccat ccagcaaagc gagcatttgc accgcaaatg tgctttgcaa
     5341 gggcgtgtat ttaaagagtt ggttgagcac ttttcggggt tgcgtgccac gtttgagttc
     5401 aacaacgata cgcatacctt tacggtcgga ttcgtcgcgc atatccgaaa tagcgtccaa
     5461 gcggccttcg cgcaccagtt cagcgatgcg ttcaatcaga ctggttttgt tgagctggta
     5521 ggggatttcg ttgatgacga tggcgtaacg cccttcgcgg atttcttcaa tgaccgcttc
     5581 accgcgcatc gtcagatgcc cacgaccggt gctgtatgcc tggcgaatgc cttcggtacc
     5641 gacgatgaca ccaccggtag gaaagtcggg ccccggcacg tgttgcatga gttcttccac
     5701 gctgacttct tcaatgtcat cccagcggtc aatcagcaaa ttgagcgcgg agaccaactc
     5761 gcccaggttg tggggcggga tgttggtcgc catacccacc gcgataccgc tggagccatt
     5821 catcagcagg ttaggtatgc gcgcgggcag gacttcaggt tccttgagcg atgcgtcgaa
     5881 attgtccaca aagtcaaccg tgtctttgtc caaatcagcg atgagttcgt cggcgatttt
     5941 gtccaggcgg gcttcggtat aacgcatggc ggccggcggg tcgccgtcga tcgagccaaa
     6001 gttaccctga ccttgcacga gcgtgtagcg catcgaaaag tcttgcgcca tgcgcgccat
     6061 cgtgtcgtag accgcgctgt cgccatgggg gtggtactta cccagaactt caccaacgat
     6121 acgcgctgac tttttgaaag gcgtgttgga gcgaataccc atgtcgtcca tggcgtaaag
     6181 aatacggcga tggacgggtt tgagcccgtc acgcacgtcg ggcaaagcgc gtgccacgat
     6241 aacgctcatc gcgtaatcga gataggccgt gcgcatttgt tcatcaatgc ctacgagttc
     6301 aacgtcacca cggttagtat ggttcgcttc tgcgttgttt tcgttttcct caggcacgtt
     6361 ttcttgttct aaattatccg gtttttctgt catattatct ttccagcctg ccaaaatttt
     6421 tataagcaag gcaactttac aagtatacca cgagcaagct gtttttcgca atttcttcgc
     6481 tgagaggcaa aaaaacgcct taaattctaa aatagcggtt ttaaaggtgg cttataaaaa
     6541 gagctttttt taatctgcaa aacgccgaaa aacgtttttt atggcatcga atgtcaagga
     6601 tatgtgttga gaaaataagc cttgtgcatt tcagtccgtt gtgattcaca gatggaatac
     6661 gccaggcttt gcaatgcttg gtataattca agcaagagaa aaggagattg aacccaacca
     6721 tgcttaaatt acgtctgatg actctcaacc ttggcggtgg catcaaaaat taccggggaa
     6781 caccccagga aagttttgat aaatccgaag cgatttcgga gctaatcaac cgcatcaagc
     6841 ctgatgtact cggtgtgcag gaaattgccc agtaccttga tggtgacggt catctgcata
     6901 gcatgactga gcgcatccgc gacgacgcgc aatttgacca cgctttttat ggggaaaccc
     6961 tgtccatgaa acagcatatg caggtcaagc aggccttgat agttgaaggc atcttcaatg
     7021 actggtggga ttggtccaaa gggaacgctt tgttctcaca tctgcctttc gcgcgtttgg
     7081 gcgacgcgac aagttcaggc atgccccgca acgtgcccgt ctttcagcca gcgcgctacg
     7141 aaggcacgcg cgacacggac ccgcgctacg tgattatctc gcgccttaaa gccagtccgc
     7201 atccttatat tctcaatctc catctaacca ccctggttgg tgaacggggc gaaaacgcct
     7261 ggtcatcaac gattgaagcg gcagaaatgg cgcgatccaa gcagatactc aatgtgctgg
     7321 gtctaatcga aaaacacgtg cttatcccgg gtgaaccggt catattaatg ggggatttta
     7381 acgccaagcc ggatgaatac acgcttaaaa atcatttggt ggaagacaat catttcgttc
     7441 gtcttgtacc cgaaaacgat atctttaccc acggaagcgc gggaacggtt gaccatatct
     7501 acttcgcgcc agcccatcgc ttgcgctcat atcaatgctg ggtggtagac gacgaactga
     7561 cccatcgcgt ttcagaccat ttgccggttg tagccgatat cgaaattgaa taactaaagg
     7621 tgattatgca aactgtaaaa tattttggca ctgatgggat tcgggggact tacggcaact
     7681 atccgatgac agaagagttt atgacccgtc ttggttacgc ggttgggacg gttttagggc
     7741 atgacgcaga cgctatcaac gaacagccca ttgtgataat cggacgtgat acgcgccagt
     7801 cagggccagg tctgcagcaa gcactcaccc gcggactgac cgcgacaggt gccatcgttt
     7861 gggatttggg catcttgcct acacccggcg tggctttttt gacccaggaa caccaggcga
     7921 ccgctggcgt ggttatttcg gcatcgcaca atccagctga acaaaacgga attaagtttt
     7981 tcagtcgcca gggcaccaag ctcagcttag agcaagagct tgaaattgaa tctatactgg
     8041 atgccaccgc ccttgaaaat gtgccagcga tggacgagag taaagtcttt gaccggttgg
     8101 atttacaagc ggattatctg cgggatttgt tggaatcgca gcacgaacta aacctgaatg
     8161 gcaagcgcat tttggtggat tgttcgaatg gcgctgcgta tcatctggca ccgcgggctt
     8221 tggaagtctt aggcgcggat gtcgtgccga ttaatattga cagtagcggt gacacaatta
     8281 ataaaaatgc cggttctgaa cgcgcgcgct ccaaacccgc tgaactgtca gcgattatgc
     8341 aagaaaaagg ggctgattta gctattgctt ttgacggtga cgcggacagg gtcatctttt
     8401 tagacgaact gggacgctta atcgatggcg accatatgct ggctattttg gcagactatc
     8461 tgcacgcgaa agacttgctt ttgggcgaca ctttggtcac caccgtgatg gcgaatggcg
     8521 cgctgaaaac ttacgcgcag aaccaaggct ttaagctgaa aaccaccccc gttggcgata
     8581 aatatgtcac cgaagcattg cagcaaatcc tggatgacac gcgcgaattg ggtaaaatcg
     8641 gcctgggcgg ggagcaaagc ggacatatta ttttgctgga ccagactcat cagaccggtg
     8701 atggcttgcg cacgatgacc ttcgttttgg cggttgtgat gcattccgac gaacaaagtc
     8761 tggcaaagct ggcagacaga attgaaaagt atccgcaact ggtagcatcg tgcgacgtgg
     8821 cgcaaaaaat cccgctggct gaactcaaag cactcagcgc gcgtctggaa actcttagcg
     8881 atgacttacc cggcctggta gaaatgaatt cgcgctattc cggcacggag aacaaatacc
     8941 gcctgatgct tgaaaccgac acacgccata ctgcccatga cctgggaaaa attgcctggg
     9001 aaatttgtgc tttggttcaa gccgaaacag gcaccccagc cggcagtaaa attgaagtcc
     9061 tgaacgtagc tgatggtggg ctaatcccca aaccataatg agaatacaag taaaacggag
     9121 ctgaatatgt ttaacgacaa accttttgta acccaactgc cttatcgctt caatcgcctg
     9181 aaccgggcga ttttggtcgc ggacaaaccc gaactttggc aagccgctat tgctgaaatg
     9241 agcgcctttt tggatgagct tgaagcctgg ctccacgcga atgtcaactt gctgggcgag
     9301 gacttagcta caagttcgcg gattttgagc ctgttgctta cccttgcggc aactggtagc
     9361 caatggcgtt tggagctttt taatgccaag aacgcgtatg agtacaagtt ccgtaagcag
     9421 ttagatcagg actatttacc taaaaacgga gcaatgcgac gtaaagcgat tgatttggcg
     9481 aagttttatt ttacagcccc cgtttttgct tctttgcagg aagacatcgc gtttgaaatc
     9541 ctgccccttt tggaaagcct ggacgccaaa caagaccccg accgcttgat ggcgtatcgg
     9601 gtgattcaaa tcggtaatat ttacgaacgc ttgtacgcca tgcgcttgcg caccgctgac
     9661 ccgctactca tcgggctggc aggcgtcaaa ggcttgttgc gcgagattta caaccgcaag
     9721 tatctgcgct ttggcacgtc gggcgttcgc ggacgctggg gcaatgactt caccgaaccc
     9781 cgcgcgcgac aagtactgca agccatttgc gacttcatga acaacgcaaa tgtgccggct
     9841 tttgtcggcg ctgaagacct ttccggacga cgtgtggtat tggggcacga cacgcgcgcc
     9901 aacgccgatc ttgtgaccca gtgggctgct gaagtctgtt tagcgaatgg ttttcgggtt
     9961 gaaatgggca cgcgcgatgt gcctactccg gcactttctt actacgaaac ggacgttttg
    10021 ccccaggatg acgttgccgg cttgattatc gccaccgcca gccacaatcc tcccgagtgg
    10081 cagggcatca aattcaatcc gcgtttgggc tatcccgcgc caaccaacgt gaccgacttt
    10141 atcgcttttc gtatcaatga gttacagttg gtggacgctg ctgggggtca ggcggatgtg
    10201 caggctgcca aggcgcgcgg tttggtgcag ggctttgacc cgctggactc atacgtgcgc
    10261 tggattaaga acaatggcca gggcaacgcc cgcattccca tcgattttga ccgtatccgc
    10321 cgctactttg ccgataagat ggtcgtggtg gatgaaatgc acggtgctgg tcgcgattac
    10381 ctgacccgtt tgattggcga agccggtgtg cgctttacgg ttatccatgc cgaagttgac
    10441 cccgatttgg gtgggcagga ttatgccaac cccgaagagc ctttcaatca gcttcttaaa
    10501 gataccgtcg ttaaaaccgg cgcgcatttg ggtatgggca tggacaccga cgccgaccgt
    10561 tttggcattg tggaccgcga cggaacttat ttcagaccca accaaatttt acctatgctt
    10621 gtgcgctatt tgggcgtgga ccgcggtttg accggtcgta ttatcgccac acaaaccggt
    10681 tcgccattgc ttgagccttt ggcgggcatg attccgggca acgaagccaa ctggccggtt
    10741 gaaggcgctt tgccgggcta tgttaaccag gtcgcttaca aatgccaggt gggcgatatt
    10801 cacacccgcg cgctgcgcta tgcttttgcg gtgccggtgg gtattaaata tattgaagaa
    10861 attcgccgca cagaccgggc atacaacgcc ttaaaagaac tgcctgataa ctggcgcgat
    10921 accatcttaa tcggtggcga agaaagctcc ggcttgacca cccgtgggca tgtgctggat
    10981 aaagacggac cctgggcaaa tattctgatc atggacatgg tcgcgtatta tggcacccgc
    11041 gagaaaaaac ccctgcactc cctgaaagaa atctgggatg aactcgtgag cctgcccggg
    11101 ctttgggaaa gctttggcac gtccacagac cccagttcgc acgcgggtcg ggctgatgtt
    11161 gacgcgccac tggaagccaa agaaggtttc atcaactact atcttgactt ggcgcaaaat
    11221 actggtgtga aaaacccaaa aatcgctgga ctgacggtcg attatttggg cggtatccgc
    11281 tatgagttgg tcgaaatgca gttaatcgat gacgatggcg acgaccatca ctacctgcgg
    11341 atgcgcgcgt cgggcaccga gccgattaat cgtatttaca ttgagtcaag ccaactggaa
    11401 acgggtcaaa aaatgatgaa agaggtgctc aaacgccttg aactcatcac catggacgtg
    11461 ctggcgaagg cccattcgcc ctggcacctg gtggatatgc ttacccaaac caccatgacc
    11521 gcggacactt tggcattagt gaaacaactc atcggagaac gtggctggga caagcaggac
    11581 attttggcta aaatacagca aatgctacag acgcttgaaa aacgcaaccg caaggtcatc
    11641 accacctggt atgaagcgtt acaaaagtag tgacaaaacg ccatagtttg tagaaaatac
    11701 tcacatttcc tggtttggca ctatccataa gttgacatct caactataat agcgcgggtt
    11761 cttaaatccc gtgctatttt gaatttaaca atgtgcgaaa gaatgcgggg taaaacattt
    11821 tgaacgagga aaaaaatgaa aattgtatgc gacagtggtg ctgatttaac cctggaagaa
    11881 tataaagaat tagacattac ggtgtttcca cttaaagttg aagtagacgg tgtttcctat
    11941 caggcgggtg tggatatcag ccccacagag ttttatgacc tgatggacaa cgcggaaaat
    12001 atgccaaaaa ccgcaacgcc ttctttgggc gatattgtag atgtttataa acgtcttgca
    12061 caggaagacc gcgatatcat ttctgttcac atttcatcgg gcttgagtgg caccagcaat
    12121 gtcgccagac aagctgcctt acttgtgccc gaggcgaaca ttactgtggt ggatacactc
    12181 acgctttcgg ccgggcaagc ctggcaggtc cgcgcggcgg cacttatggc taaagccaac
    12241 aagaaccgcg aagagatact ggaaaaactg ggccaaattc aacaggcttt gacttcacat
    12301 tttaccttgc cggatttgaa gtatctcatt gccggcgggc gtatcggcca cttgaaaggt
    12361 cttctggctt ctttgctggg catcaagccg attattgaag taaacaaaga agacggtaaa
    12421 tactacgatg tcggcaagcg tcgttctttc caaaaggcga ttgataatat tcccgaactc
    12481 ctgctaaagt accatcaggc tggcgcgcaa ctgcgggcgc aaattgtgac cgctgccaac
    12541 tccgaaggtg gcgaaaaact caaacaggcg atggaaaagg tcttcaacgt gagctgggag
    12601 cccgaggtcg tactcggcac cgcgct
//
LOCUS       DAOSFC010000005        12495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_4234_length_12495_cov_4.664871, whole genome shotgun sequence.
ACCESSION   DAOSFC010000005 DAOSFC010000000
VERSION     DAOSFC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4234_length_12495_cov_4.664871"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..694
                     /gene="lspA"
                     /locus_tag="PK791_00285"
     CDS             122..694
                     /gene="lspA"
                     /locus_tag="PK791_00285"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOH91783.1"
                     /translation="MKEKVKHNAFLIVVMTLIVALDQWTKLLVMHKFEVGEGIAPIPA
                     LGDFFRIIHWRNTGAAFGLFQNANTVLLVLSIIIVIAVLYYYQLVKANAWLAKVSLAL
                     ILSGAIGNIIDRIKLGYVVDFLSFGRFPVFNVADSAVTVGVFLLAIQYMLEERKALKQ
                     KEEEQTEKTCKECATDKEIEEVVYKVEDTE"
     gene            691..1614
                     /locus_tag="PK791_00290"
     CDS             691..1614
                     /locus_tag="PK791_00290"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH91784.1"
                     /translation="MSPSFEKHVFEAAEAGRLDKLISDTLRDVSRRRAQTAILDGQVR
                     LNGEICLKASTRVEPGDKVEISLALEREDVLLAENIPLNVIYVDENVIVINKNAGMVV
                     HPGAGNPSGTLVNALLYKWPELREAGEAGRPGIVHRLDKDTSGVLIVARNNSSYKWLV
                     RQFKSRKVIKKYLALLDGHPPTPSGRIEANIGRDDTHRQRMTVKYGQSRAAISEYQTI
                     QTYPRHTLVEVQPHTGRTHQIRVHMAFIQAPITGDRVYGRRKLSVPIERFFLHANKLG
                     IILPGDKQITEFVAPLPDDLKEILKTVEKMN"
     gene            1614..2654
                     /gene="ltaE"
                     /locus_tag="PK791_00295"
     CDS             1614..2654
                     /gene="ltaE"
                     /locus_tag="PK791_00295"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOH91785.1"
                     /translation="MKTFDFRSDTVTKPSDAMREAMAKAVVGDDVYHEDPTVNRLEAM
                     TAELLGKEASLFVSSGTMGNLVAVLAQCQRGDEVIMGTQGHTFLHEAGGVSVLGGVSM
                     FTVPNDADGSIPLAGLETAVRNHFDVHEPITKLVILENTQNFCGGAVLNPFYVSEVAE
                     FCKCRGLGLHIDGARLFNAAVELNMPVSEVVKEADSVTFCLSKGLGAPVGSMLSGTKA
                     FIQKARRLRKILGGGMRQVGILAAAGIYALEHHVERLRIDHVRAKQLASGISELPGVQ
                     ITRDTPHTNIIFFALDDTVEIDTPTFLKKLAEKGVLIMDVGPREFRMVTHLEISDEGV
                     GQCLQAFKDILG"
     gene            2708..4831
                     /locus_tag="PK791_00300"
     CDS             2708..4831
                     /locus_tag="PK791_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91786.1"
                     /translation="MKNAKLLTLLLTIMLALAFFSFNGDTPYALANADAEDSQPEAAE
                     PGVDRLYIYSFPIMKNGNALLDPVGPIGGTFTSVLTDPSDSNVVIGGHYEAGVFISFN
                     QGNTWYARNNGLPNLKIQSLAIHPRHSNILYAGTYGNGVFLSRDRGLNWIPWSGGVLE
                     NHIIYDIAIDPTSPSVVYTTSRVDRSLMGYICRSTDAGKTWRVVYRGDWFDSLDYFYE
                     VEVHPSGINTVYFSAHEHGFYRSVDNGLNFSPINAGVSDLSARGLAMNRANQNVILGG
                     VWKGGGIYKSTNLGASWQSSRTGLPDSAKITKIKADPNDAYGNRYFATTYTNGLYVSE
                     NSGGSWQNRGWNGSHINDIAISPYYPQTWFMATQYSGMVRTRDGGASWSTIMDDLRLY
                     SVTGIQSLGDDPDALYVSIYGMGVYRYVPTENSWQAVNSGLASLEINVLHNDGKSLWA
                     VAQDGLWRFDGDKWQSVFLPLASAGNVDALKSWQASRLGEPFVEQVYRLAPQDNALEN
                     TGDLNHLQTTALGSLGDLLYLGTDNGVWLKNAYRWTYLGLDRKVVYTMAIDEKTQSLW
                     VSACGTQNDCQVFQYFEGEWFERSQGLNGAQINQLLFADETLLAVGEMGIHRWDAANK
                     AWKLVYQVSDGLLSITQNPNEPKQLLAGGNGIVVYSDDFGENWQEFYTELTWTYPVLA
                     FSPGENVKFLLGSIESGPFIMEIEQ"
     gene            4886..6268
                     /locus_tag="PK791_00305"
     CDS             4886..6268
                     /locus_tag="PK791_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH91787.1"
                     /translation="MLIILSDLHLADTSPYNLGINRSNLNLPGRVYRTFFREIAELIK
                     DGDVKHIDLVLAGDIFEITRSVLWHQGDLRPYVHNDEVTAGSPHERRILDILDAIVLD
                     PKVGDTAKVFQSLPELFNLPVNIHYVPGNHDRLANASVAIRNKVRAFFGVPESDEPFP
                     NQYLHYRDDEVRVLVRHGHEYDHSNFGANLAMWPEIPLIIDKSYYDMPVLGDIVTMEI
                     ASKLPLLFRERYGDDEILGDTHLKRMYQRLIDFDNVRPNGAIMSFLFSTPGLSTKEIW
                     LIIEPVFRDLLDDIAKNDDLAEDIVLLGNFKRFSAKAIASILKARVWRRGMPIWMFNA
                     LVNPAAKKSGVDADMDIIVKEQFLKQNDNDLRCLVTGHTHNAIVRLLSVDDGVEKYYL
                     NSGTFRNVIMPTPGYDKFGRLRSKARLLIYEEGEQNPEYTRETGWSFDFNARLGFGAE
                     AENLIDNLAD"
     gene            complement(6257..6550)
                     /locus_tag="PK791_00310"
     CDS             complement(6257..6550)
                     /locus_tag="PK791_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91788.1"
                     /translation="MLKNKIIEIFMLIPFLKKVLTDSNAEEVRRVENILIEHKIPYRI
                     RTYKTRGSIGMALDTSSYARSNLAMYKGASSPSISYGIYVRKKDYQRVMDLIS"
     gene            complement(6574..9369)
                     /locus_tag="PK791_00315"
     CDS             complement(6574..9369)
                     /locus_tag="PK791_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH91789.1"
                     /translation="MRPYQSQNTDDIMIIRRSKFLTLVQTAITNREFQFVRQACLIWM
                     ASYPGDLLVNFIYASALVELGDTEMALATLERLLRFDPEFTEALSLYSRLSGKSNVEN
                     ETALAYLTRSVSLDKTLSPWLPALNQARSEFESGDYNAAEKSVLESLAQNPSSPLPAI
                     MHLNIVLQMSNATLLKTLTELYSSRWKDCLQIKIFSALADMSNEKDSAGVEKLHWAAA
                     HDVSGQVVHRLLGPNHVYQPLWPEDLKVYLDLPVPASVAADLGWNILATASSSSHSAP
                     ATQQVSDTEATQAIAHSTMSAEDALQALLSEQATVPLKPSNLLNILQSDENIAVDYSE
                     IPTASFFKQDIATPPSNVAFSEQHFEESLFTQETRQTVAALDEIQSDFDQVTRKIHRP
                     ELSNADTRFPSYVLFTSRKNLATKYGENTAQVIIDSIKSLSAKILALPGWNSTVFIPD
                     DAEISTELGTTQIESCDANTLKSALVDLDAKLSQRGEMIGALLIVGGNDIVPFHLLPN
                     PTDDSDPNVPSDNPYATLDENYFVQQWPVGRLPDEKGKDAGYLIEQLRFLNNEYALKR
                     KSKKSAFFNVLSSLFVTLFSWLRVFGTKPSNIESLGISAEVWKRASEGVYGVIDDNKR
                     LLTSPPTNNKNLLESRKASSQFAYFNLHGLKDTNEWYGQRDLSREGTDIEYPVALNTK
                     NFTQESGAPAIVLSEACYGAWVFDKKVNDSVALNFLSRGTRAFVGSTVIAYGAPGKTL
                     VAADLLAYHFWSHVKKEVAVGYALMRAKLALASKMTADQGFLDGEDQKTILSFVIYGD
                     PLASSKDIKEITKPIVRVATSPVIKTISDSRPDMIINQDEMPNEILNKVRKVIKSYMP
                     GMEEGKMAISPLLGVIQSTSDPATDKKNTLEAIKQSQRYVVTVKKSHEYRSKNFDQFA
                     RMTFDQKGKMVKLSTSK"
     gene            complement(9366..>12495)
                     /locus_tag="PK791_00320"
     CDS             complement(9366..>12495)
                     /locus_tag="PK791_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH91790.1"
                     /translation="DRNATYDASFEMKLLEADVKARLGELNEAYEDYLYLLDLPEAKA
                     PRFYQHLQRQIGLVALDLGYDDISMASLQEAISYNPNDLASRHALSHAYLKSGLNDKA
                     IESAQTALELAPADTHNVLWYSKFMQANHRIHDAIQALKDAIFRRPEEQILHLSLARV
                     YLANNAVDESKITLSKMLETSDIPTEDYLHIAGLYARMNETQLAVDILQRAITENENP
                     SFVESCQITYAFLEHQQNNIALNFLDHIKQKHGQQAGYAMLRADVLSAEKQYLSALEA
                     LEPLLRRLESGAIESIQSAFIPEENSGILPLDTEGLYLRVAYLNRLLANYEVAGKYAK
                     LALKTNESSKNARILCLDLALASCDKASLDQTLEELNRKESLNEIELELAQRLALDAL
                     LEKDLTKASLIYEQFLTPVEEELIPMATKAMISEHEGQAHQAMAFIQVAKEGFSTTDN
                     SGEKPLSLAEQFSKVWQSLALALVAWKLQDWAFANQLFSQATSICKSNPRLNLVFADY
                     LADKARMQRTCLNLRVKRHLPQAFTSDRSDESVFDEQIALAKANLEPSLTQAIEIIGK
                     AQFAGHLDDSSQLDARVKNSHIAKQAIAVLKDTVSISKILAMYPHDNELQFQYALQNI
                     DKQADLACEILNSIPIKPNLQALHHAALALAQVSEPELAGEAINRALNIWQDEAEWFI
                     LRAEIQEKQVKLQDATQSVKLALELEPNNPGYWQYLGELALREGDLHTARNHFAKANS
                     LKYNTIPVIEALADINRQLGDYPEAIAYYKELHILEPNNDSYLESLAELYFIIQDYDL
                     ALETADKVINRSLNCERALKVKIETLISRQAFDEAKKLANDAMLIAKDPITFEIYRIR
                     IEARENPANGLRMATNLAHEHPDYPNVLNLLAQYQISLDQKQNAEKTLIRSLQLDKSN
                     PETLLALGALNRQNNSHETAQTYLKQALELNPSLIEAYLELGQSLQDQRLNDQALQVY
                     NKAIEQVSKDPRPYVHAANAYKASRDFRSAELMLKQASQLAPSDQSIRRQLAAVVAQN
                     LLDNLQEAPKRK"
BASE COUNT         3025 a         2560 c         3369 g         3541 t
ORIGIN      
        1 ttaccagcga gactttggcg cttgagttag aatcatctga gaacccacaa ggtcttgcca
       61 gcgctgaaga tcgctttgac gagcaagaca taataattgc gcttgaaaag gttgaacgtg
      121 aatgaaagaa aaggtcaaac ataacgcatt tttaattgtt gtcatgacgc tgattgtggc
      181 gttggaccag tggacgaagc ttttggttat gcataagttt gaggtgggcg agggcatcgc
      241 gccgatacca gctttgggcg atttcttcag gattattcac tggcggaata caggcgccgc
      301 ttttggctta tttcaaaacg ctaatacggt attgctggtc ttgagcatca ttattgtgat
      361 tgctgtgctc tattattatc agctggttaa agcgaacgcg tggctggcga aagtgtcgct
      421 ggcgttaatc ctttctggcg cgatagggaa catcattgac cggattaagc tgggctatgt
      481 cgttgacttt ttgtccttcg ggcgttttcc ggtgttcaat gtggcggatt ccgcggtgac
      541 ggtaggcgtt ttcttgctgg ctattcaata tatgctggaa gaaagaaaag ccctaaagca
      601 aaaagaagaa gagcagaccg agaaaacctg caaagaatgc gcgactgata aagaaattga
      661 ggaagttgtt tacaaagttg aggacactga atgagtccca gttttgaaaa gcatgttttt
      721 gaggctgctg aagcgggccg tttggacaag ttaatcagtg atactctaag agatgtttcg
      781 cgcaggcgtg cccagactgc tatccttgac ggacaggtaa ggctgaatgg tgagatatgc
      841 ctgaaagcgt ctacccgcgt ggaaccggga gataaagttg agattagctt ggcgcttgag
      901 cgtgaggatg ttctactcgc tgaaaatatc ccgctgaacg tcatttacgt tgatgaaaat
      961 gttattgtga ttaacaagaa tgccggcatg gtggtacacc cgggtgcagg caatcccagt
     1021 ggcaccctgg tcaacgcttt gttgtataaa tggcccgaac tccgcgaagc aggcgaagca
     1081 gggcgaccag gtattgtgca tcgcctggac aaagacactt ccggcgtatt gattgtcgcg
     1141 cggaataact cgagttacaa atggctggta aggcaattta agagtcgaaa agttattaag
     1201 aaataccttg ccttgcttga tggtcatccg ccgacaccca gcggtcgcat tgaagcaaat
     1261 attggcaggg atgataccca tcgtcagcgt atgacggtga agtacggtca aagccgcgcg
     1321 gcaatttcgg aatatcagac gatacaaact tacccaagac ataccctggt cgaagtgcaa
     1381 ccccataccg gaaggacgca tcaaatccgg gttcacatgg cattcattca ggcgccgatt
     1441 acgggcgaca gagtttatgg gcgacgcaag ttgagcgttc ccattgagcg tttctttttg
     1501 cacgccaaca aactgggtat cattctgccg ggtgataaac aaataacgga gtttgtcgcg
     1561 ccattgcccg atgatctcaa ggaaatattg aaaacagtgg agaaaatgaa ctaatgaaaa
     1621 ctttcgattt tcgctcagat accgttacca aaccatccga cgccatgcgc gaggcgatgg
     1681 caaaagccgt cgttggtgat gacgtttacc acgaagaccc aacggtcaat cgacttgaag
     1741 caatgaccgc ggagcttctg ggaaaggaag cgtcactgtt cgttagttcg ggcacgatgg
     1801 gcaatctggt tgctgtcctg gcgcagtgcc aacgaggcga cgaagtgatt atgggtaccc
     1861 agggccatac ctttttgcat gaagccggtg gtgtttcggt tttgggtggt gtgtcgatgt
     1921 ttactgtgcc caacgatgct gatggcagta ttccgctggc gggtcttgag actgcagtgc
     1981 gcaatcattt tgatgtgcat gagcctatta ctaagctggt gattctcgaa aacacgcaaa
     2041 atttttgcgg tggagcggtg ctgaatcctt tctatgtatc cgaagtggct gagttttgta
     2101 aatgccgtgg tttgggtttg catattgatg gcgcgcgttt gtttaatgcc gcggtggaat
     2161 taaatatgcc tgtttccgaa gtggtgaagg aagctgattc ggttactttt tgtctcagca
     2221 aaggacttgg cgcgccggtt ggctcaatgt tgagtggtac aaaagcgttt atccaaaaag
     2281 cacgtcgatt gcgcaagatt ttgggtgggg gcatgcgtca ggtgggtatc ctcgccgccg
     2341 cgggtatcta tgccctggag catcacgtgg aacgcttgcg cattgaccat gtacgtgcca
     2401 aacagctggc atctggcatc tcagaactcc caggcgtgca gataacgcgg gatacacccc
     2461 atacaaacat tattttcttt gctttggatg atactgtgga aattgatacg ccaaccttcc
     2521 ttaagaaact ggctgaaaaa ggtgttttga ttatggatgt gggaccaaga gagtttcgaa
     2581 tggtgacgca tttggaaatt agcgatgaag gtgtagggca gtgtctgcaa gcgtttaagg
     2641 acattttagg ctagtctata cataatacct ggcattctac ttgcacttta attgccagag
     2701 aatgcctatg aagaacgcga agttactcac attattgctg accataatgc tggctttggc
     2761 gttcttttct tttaacggtg atacgccgta tgcacttgca aatgcggatg cggaagacag
     2821 tcagcctgaa gcagccgaac cgggtgttga tcggctttat atttacagtt ttccgattat
     2881 gaaaaatggc aatgcattgc ttgacccagt aggtccgatt gggggaacgt ttacgtcggt
     2941 attgactgat ccatcagaca gcaatgtggt catcggcggg cactatgaag caggcgtgtt
     3001 tatcagtttt aaccaaggca atacctggta tgccaggaat aacggtttgc caaatttaaa
     3061 aatacaatca ctcgccattc atccgcgcca ttctaatatt ctgtacgctg gtacctatgg
     3121 caatggtgtt ttcctttcgc gggacagggg cttgaactgg attccgtgga gtggtggcgt
     3181 gcttgaaaac catatcattt acgatatcgc gattgacccc accagcccat ctgttgttta
     3241 cactacatcc cgtgttgaca ggagtttaat gggctatatt tgccgttcga ctgacgcggg
     3301 caaaacctgg cgggttgttt atcgcgggga ctggtttgac tctttggatt atttctacga
     3361 agttgaagtt catccatcgg ggattaatac ggtttatttt tcagcgcatg aacatggatt
     3421 ttatcgcagt gtggataatg gactcaattt ctcgccgata aatgcgggcg tgagtgactt
     3481 gtccgcgcgc ggtctggcga tgaacagggc taaccaaaac gtgattctgg gtggtgtttg
     3541 gaaaggtgga ggcatctata agagcacaaa tttaggggcc tcgtggcaat cttcgcgaac
     3601 tggtttgccc gatagcgcga aaattacgaa aatcaaagct gacccaaacg atgcctacgg
     3661 taaccgttac ttcgcgacga cctataccaa cggcttgtat gttagtgaaa acagcggtgg
     3721 ctcatggcaa aaccgaggct ggaatggcag ccatattaat gatattgcga tttcacctta
     3781 ctatccccaa acctggttta tggcaacgca atattcaggc atggtgcgta cccgcgatgg
     3841 gggcgcgagc tggtcaacga ttatggacga cttgcgcctg tattcggtta cggggataca
     3901 gtctttaggc gatgatccgg atgccttgta tgtttctatt tatggtatgg gcgtgtaccg
     3961 ttatgtgcct accgaaaaca gctggcaggc ggttaattct ggtctggcaa gtctggagat
     4021 aaatgtttta cacaatgatg gcaagtcgct ttgggcagtc gcgcaagatg gcttatggcg
     4081 ttttgatggt gataaatggc aatctgtgtt cttgcctttg gcgtctgcgg ggaatgtgga
     4141 cgcgctgaaa agctggcagg catcccgcct gggagagcca tttgttgaac aggtgtatcg
     4201 cctggcacca caagacaacg cgctggaaaa tactggtgac ttaaaccatc tgcaaacaac
     4261 cgctttaggg tcactgggtg atttgcttta tcttggaacg gataatggcg tttggctcaa
     4321 aaatgcttac agatggacgt atttaggact tgatcgaaaa gttgtttaca caatggcgat
     4381 tgatgagaag acacagtcgc tttgggtttc tgcttgtgga actcaaaatg actgccaggt
     4441 ttttcagtat tttgaaggtg aatggtttga acgcagccaa ggcttaaatg gtgcacaaat
     4501 caatcagttg cttttcgcag atgaaacact gttagcggtt ggagagatgg gaatacatcg
     4561 ctgggatgct gcgaataaag cgtggaagct ggtgtatcaa gtgtctgatg gcttgctgag
     4621 cattacgcaa aatccaaatg agccaaagca attactggca ggcgggaatg ggattgttgt
     4681 gtatagcgat gactttggag aaaactggca ggaattttac acagaactga cctggacata
     4741 cccggttttg gcattcagcc caggtgaaaa tgtaaagttc ttgttaggaa gcatcgaatc
     4801 cggccctttt atcatggaaa ttgaacaata atcgactaca atttttctaa gttggaatgc
     4861 agctaagaga aatggagcgg aaaccttgtt aatcattctt tcagatttac accttgctga
     4921 cacaagccct tacaatttag gtattaaccg cagtaatctc aacctgcctg gacgggttta
     4981 ccgtactttt ttccgggaaa ttgctgaact tatcaaggat ggtgatgtaa aacacatcga
     5041 tcttgttctg gcgggggata tttttgaaat tacacgttct gttttgtggc atcagggtga
     5101 cttgcgccct tatgtacata atgacgaagt taccgcgggt tctccacatg aacgacgtat
     5161 cttagacatt ctcgatgcga ttgttcttga ccccaaggtt ggggatactg ccaaggtgtt
     5221 ccaaagctta ccagagcttt tcaatttgcc tgtaaatatt cactatgtcc caggcaatca
     5281 tgaccgtctg gcaaacgcgt cagtagcaat acgcaacaaa gtccgtgctt tttttggtgt
     5341 tccggaaagc gatgagccat tcccgaacca gtatttgcat tatcgcgatg atgaagtgcg
     5401 ggtattagtg cggcatgggc acgaatacga ccattcaaat tttggcgcta atttagctat
     5461 gtggcctgaa attcccttaa tcattgacaa aagctattac gatatgcctg ttttggggga
     5521 cattgtaacg atggaaatcg ccagcaaatt acccctgctt ttccgggaac gttacggtga
     5581 tgatgagatt ctgggcgata cgcatttaaa gcggatgtac caacgcctga ttgactttga
     5641 taacgtgcgt ccgaatgggg caatcatgag ctttttgttt tcaacaccag gcttgagtac
     5701 caaagaaatt tggctgatta ttgaaccggt ttttagagat ctcttggatg atatcgcgaa
     5761 gaatgacgat ttagctgagg atattgtctt gttaggcaat ttcaaaaggt ttagcgcaaa
     5821 ggcgattgcc agtattttaa aagcccgtgt ttggcggcgg ggaatgccga tttggatgtt
     5881 taacgcactt gttaatccag ccgcgaaaaa atcaggcgtt gatgccgaca tggatattat
     5941 tgtgaaagaa caattcctaa aacaaaatga caatgatttg cgttgtttag taactgggca
     6001 tacgcataac gcgatagtcc gtttgttgag cgtggatgat ggtgttgaaa agtattattt
     6061 aaacagcgga acttttcgta atgtgattat gccaacccct ggctatgata aatttggcag
     6121 attacgttca aaagcgcgct tgttgattta cgaagaaggt gaacagaatc cagaatatac
     6181 ccgcgaaacg ggctggtctt tcgatttcaa cgctcgctta ggctttggtg ctgaagcaga
     6241 aaacttaatt gacaacctag ctgattaaat ccattactcg ctgataatcc tttttacgga
     6301 cataaatgcc atagcttatt gatgggctgc ttgcaccttt atacatagcc aggttcgaac
     6361 gcgcataaga actggtatct aatgccatgc cgatgcttcc acgtgtttta taggttctga
     6421 tgcggtaggg aatcttatgc tcaatgagta tattctccac acgacgaact tcctccgcgt
     6481 ttgaatctgt caataccttc tttagaaaag gaatgagcat gaaaatttca attattttat
     6541 ttttcagcat agcttaatgt ctcctaatct gttttacttg gaggtgctaa gcttgaccat
     6601 tttgcctttt tggtcgaagg tcatacgggc aaactggtca aagtttttag agcgatactc
     6661 gtggcttttc ttcacggtaa ccacatagcg ttggctttgt ttaattgctt ccagggtatt
     6721 ctttttatct gtagcagggt cggaggtgga ttgaatgaca cccaggagcg ggctgatcgc
     6781 catttttcct tcttccatgc ctggcatgta ggatttgatt accttacgga ctttgttcag
     6841 gatttcattt ggcatttcat cctggttgat aatcatatct ggacgtgaat cgctgatggt
     6901 cttaatcacg ggcgatgttg ccacgcgtac gatgggttta gtgatttctt tgatgtcttt
     6961 actgctggca agcgggtcac cgtaaatgac gaaggagaga atggtttttt ggtcttctcc
     7021 gtccaggaag ccttggtcag cggtcatttt gctggcaagg gctaatttcg cacgcatcag
     7081 cgcgtaaccc acagcgactt cctttttaac gtgcgaccaa aaatgataag ctaaaaggtc
     7141 agccgcaacg agggtcttgc ctggcgcgcc ataagcgata accgttgagc caacaaaggc
     7201 acgtgtgcca cgtgacagga aattgagagc aacggaatca ttgacttttt tgtcaaaaac
     7261 ccaggcacca tagcaggctt cgctcaggac gatggcgggc gcgccacttt cctgagtgaa
     7321 attcttggtg ttaagcgcga cgggatattc gatgtcagtg ccctcgcgac ttaaatcacg
     7381 ctggccgtac cattcgttcg tatctttaag accatgcagg ttgaaataag cgaattgtga
     7441 actggctttg cgagattcaa gcaggttttt attgttggtt ggaggtgatg tgagcagacg
     7501 cttgttgtca tcgataacac cgtacacgcc ttcagaagcg cgtttccaga cttccgcgct
     7561 gatgcccagg ctttcaatgt tgctgggctt tgtgccaaag acgcgtaacc acgaaaacag
     7621 ggttacaaaa agactgctaa gtacgttgaa aaaggcactc tttttggact ttcgtttcaa
     7681 tgcgtattcg ttgttaagaa aacgcagctg ttcaatgagg taaccagcat cttttccctt
     7741 ttcatcggga agccgaccga caggccattg ttgcacgaaa tagttctcgt caagtgtggc
     7801 ataagggtta tcggaaggaa cgttggggtc actgtcgtcg gttggattgg gcaaaaggtg
     7861 gaacggtact atatcattac caccgacaat aagcaaagcg ccgatcatct cgccacgctg
     7921 ggagagtttc gcgtccaaat ccaccaaagc agattttagg gtattggcat cgcagctttc
     7981 gatttgcgtt gtgccgagtt ctgtactgat ttccgcgtca tcgggaatga atacggtact
     8041 gttccagccc ggcagggcga gtattttagc tgagagactc ttgatagagt cgataatgac
     8101 ctgggcggtg ttttcgccat atttggttgc aaggtttttg cgactggtaa acaatacata
     8161 gcttgggaat cgtgtatccg cgttggacaa ttccgggcgg tgaatttttc gcgtcacctg
     8221 gtcaaagtca gattgaattt cgtccagtgc ggcgacagtt tggcgggttt cctgagtgaa
     8281 gagactttct tcaaagtgtt gttcgctgaa tgccacatta ctgggcggtg tggcaatgtc
     8341 ttgtttgaag aaagaggctg tggggatttc ggaataatcc acggcgatat tttcatcact
     8401 ttgtaatata ttgagcaaat ttgaaggctt tagtggaaca gtagcttgtt ctgaaagcaa
     8461 cgcctgaagg gcatcttccg cggacattgt gctgtgcgca atggcttgtg tggcttctgt
     8521 atctgatact tgttgagtgg caggggcact gtgagacgaa gatgaagcag ttgccaggat
     8581 gttccaacca aggtcagcag cgacactggc gggtacgggc aggtcgagat agactttgag
     8641 gtcttcaggc cataaaggct gatagacatg gtttgggccc agcaagcggt gcacgacctg
     8701 tccgctgacg tcatgggcag cagcccagtg tagtttttca acgcctgctg aatctttttc
     8761 gttactcata tcagcgagcg cggagaagat tttgatttgc aaacaatctt tccagcgact
     8821 tgagtagagt tcggtgagtg tctttaacaa tgtcgcgtta ctcatttgca agacgatgtt
     8881 taaatgcata atagctggca ggggcgaaga tgggttttgg gcaaggcttt caagcacgga
     8941 tttttccgcg gcgttatagt cgcctgattc aaattcagaa cgcgcttggt tgagcgcggg
     9001 aagccaggga gacagggttt tgtctaggct gacactgcgg gtgagatatg ccagcgcggt
     9061 ttcgttttcg acattggatt ttcctgaaag gcgggagtaa aggctaagcg cttcggtaaa
     9121 ttccgggtca aagcggagaa gacgttccag cgtggcaagt gccatttctg tgtcgcctaa
     9181 ttcgactaaa gcagaggcgt agatgaaatt gaccaacaag tcccctggat aacttgccat
     9241 ccaaattaaa caagcctgac gaacaaactg gaactcgcga ttggtgatcg ctgtctgtac
     9301 gagcgtcagg aatttgctgc gtcggattat catgatgtca tcggtgtttt gggattgata
     9361 tggtctcatt ttcttttcgg tgcctcttgt aggttatcga gtagattttg tgcaactacc
     9421 gcggcaagct gacgacggat gctttggtcg ctgggggcaa gttgagaagc ctgtttgagc
     9481 atcaactctg cactacggaa atcgcgactg gctttgtaag cgttggcagc gtgcacgtag
     9541 ggtcgggggt ctttgctaac ttgttcaatg gccttgttgt aaacttgcag ggcctggtcg
     9601 ttcaggcgct ggtcttgcag gctttggccg agttccaaat aggcttcaat aagacttggg
     9661 ttgagttcaa gcgcttgttt gaggtaagtt tgcgcggttt cgtggctgtt gttttggcgg
     9721 tttagagcgc caagcgcgag aagcgtttcg gggttgcttt tatcgagttg taagcttctt
     9781 atcaaagtct tttccgcgtt ttgtttttgg tcaaggctta tctgatattg cgcgagcaga
     9841 ttgagcacgt ttggataatc tgggtgttca tgcgccagat tggtggccat gcgcaagcca
     9901 ttggctgggt tctcgcgggc ttcaattctg atgcggtaga tttcaaaggt gatagggtct
     9961 ttggcaatca acatggcatc atttgccagc tttttggctt catcaaaggc ctggcgcgag
    10021 atgagtgttt caatttttac tttcaacgct cgttcgcagt tgagcgagcg attaattact
    10081 ttatcagcag tttccagggc taaatcatag tcctgaatga tgaagtaaag ttcggctaaa
    10141 gattccagat aactgtcatt attgggttcg agaatgtgca gttctttgta atatgcgatg
    10201 gcttccgggt aatcaccgag ttgacggttg atgtctgcca gggcttcaat gacgggaatc
    10261 gtgttgtatt tgagcgagtt ggccttcgca aaatggttgc gggcggtgtg gagatcacct
    10321 tcacgcagag caagttcgcc taagtattgc cagtagcctg gattattggg ttccagttca
    10381 agggcaagtt ttacgctttg tgtggcgtct tgcagtttga cttgtttttc ctgtatttct
    10441 gcccgcagga taaaccattc agcttcgtct tgccaaatgt ttagggcacg gttgatggct
    10501 tctccggcaa gttcaggttc gctgacttgc gctaatgcta aagcagcatg gtgcaaagcc
    10561 tgaaggttgg gcttaatggg aatgctgttg agtatctcgc aggctaaatc ggcttgtttg
    10621 tcgatatttt gcagggcata ttggaactgg agttcattat catgcgggta catggccaga
    10681 attttagaaa tactgacggt gtctttgagc accgcgatgg cttgtttagc gatatggctg
    10741 tttttaacgc gtgcgtcaag ctgactgctg tcatcaaggt ggccagcaaa ctgtgctttg
    10801 cctatgattt ctatggcttg tgtgagactg ggttccaagt tggctttggc tagcgcgatt
    10861 tgctcatcaa agaccgattc atcacttctg tcggaggtga acgcctgcgg tagatgtcgt
    10921 ttgacccgca aattaaggca agttctttgc atacgggcct tatctgccag gtaatcggca
    10981 aagaccaggt tcaagcgcgg atttgatttg caaatgctgg ttgcctgact gaaaagctga
    11041 ttagcgaatg cccagtcctg tagtttccag gctacaaggg caagtgccag ggattgccag
    11101 actttgctga attgttcagc caagctcagt ggcttttcgc ctgaattatc agttgttgaa
    11161 aaaccttctt tggcaacttg aatgaatgcc attgcctgat gtgcctgacc ttcatgttcg
    11221 ctaatcatcg ctttcgtcgc catggggatg agctcttctt caactggcgt aagaaattgt
    11281 tcgtatatca gactggcttt ggtcaaatcc ttttccaaaa gcgcgtccag agccaggcgt
    11341 tgggctaatt caagttcgat ttcgtttaga ctttctttgc ggtttaactc ttccagggtt
    11401 tggtcaagac tggctttatc gcaggaagcg agtgccagat ccaggcagag aatacgggcg
    11461 tttttgctgc tttcgtttgt cttaagggcg agcttggcat attttccagc cacctcatag
    11521 tttgcaagca agcggttcag gtaagcgaca cgcaggtata agccttccgt gtcaagtggc
    11581 aggatgccgg aattttcttc gggaatgaaa gcgctttgaa tgctttcaat ggcgccactc
    11641 tcaaggcggc gcaataaggg ttcgagcgct tccaaagcgg acaggtattg tttttcagcg
    11701 cttaggacgt cagcacggag catagcatag ccagcttgtt gaccgtgttt ttgttttata
    11761 tggtcaagga agtttaaggc gatgttgttt tgctgatgct caaggaaagc ataggtgatt
    11821 tggcagcttt caacaaagct ggggttttca ttttcagtga tagcgcgttg taagatatcc
    11881 accgcaagct gggtttcgtt catgcgcgcg tagagcccgg caatgtgcag gtaatcttcg
    11941 gtggggatgt cggatgtttc cagcattttg gacaaggtga tttttgattc atcgacggcg
    12001 ttattggcca ggtaaacccg cgccagtgaa agatgcaaga tttgttcttc agggcgacgg
    12061 aaaatcgcgt ctttcaaagc ttgaatggca tcatgaatgc gatggttggc ttgcatgaac
    12121 ttgctgtacc aaagcacgtt gtgagtgtcg gcaggggcaa gttccagagc ggtttgggcg
    12181 ctttcaatcg ctttgtcatt gagaccggat ttcaggtatg catgtgagag agcatgacgg
    12241 gaggcaaggt cgttggggtt gtaggagatg gcctcctgca gcgatgccat gctgatatcg
    12301 tcatagccca agtcaagcgc gacaaggcct atctgacgtt gcaagtgttg gtagaaacgc
    12361 ggggctttcg cttcgggtaa gtcgagcaag tagagataat cttcgtaagc ttcgtttagt
    12421 tcaccaagtc tggcttttac gtcagcttcg agtaatttca tttcaaaact ggcatcatag
    12481 gtggcattgc ggtcc
//
LOCUS       DAOSFC010000006        12270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_4348_length_12270_cov_4.802374, whole genome shotgun sequence.
ACCESSION   DAOSFC010000006 DAOSFC010000000
VERSION     DAOSFC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12270
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4348_length_12270_cov_4.802374"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..2083
                     /gene="tkt"
                     /locus_tag="PK791_00325"
     CDS             71..2083
                     /gene="tkt"
                     /locus_tag="PK791_00325"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOH91791.1"
                     /translation="MNKTDIEQALNTIRFFSADGVQLANSGHTGMPMGAAALAYTLYV
                     RQMNHNPKNPNWINRDRFVLSGGHASMLLYTILHLTGYDISIDDLKQFRQYQSITPGH
                     PEWGVTPGVDTSTGPLGQGFATGVGMAIAEAHLAATFNKPGFDIVNHYTYAIVTDGDM
                     MEGISSEAASLAGTLKLGKLIYLYDSNDITIEGRTDIALTEDVAKRFEAYNWHVQTVE
                     DGNDVEAIDKAIEAAKLDPRPSLIICKTIIGYGLPNRADTSGIHGSPAGWDELNLAKE
                     NAGIPTEPLFYITDEILAHFREQIAVGAELQKEWNELYTCYKEKYPDLAKEFERRNAR
                     ILPENWDENLPVFEPDKKGLATRVASWHTIQKIADALPEVVGGSADLAPSNNTWMDAY
                     PAFGPEQRDGRNIHYGVREIAMAAIGNGLFVHGGIRPFNATFLVFSDYLRGSLRVSAL
                     SNIANIFVLTHDSIGVGEDGPTHQPVETIMSLRLMPNLNVIRPADANETVEAWKAAIE
                     KTDGPTALILSRQNIPTIDRQKYASAKGLHKGAYVLYKSKDGTPDLIIIAAGSEVTLA
                     LDCISALEEKGLNTRVVSMPCWELFEAQDKVYKDEILPPSVKNRISLEAGQTLGWERW
                     VGQYGVSLGVTEFGLSAPGDIIFKEFGFTVENVISMIEKMMKKNQG"
     gene            2083..4854
                     /locus_tag="PK791_00330"
     CDS             2083..4854
                     /locus_tag="PK791_00330"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HOH91792.1"
                     /translation="MEMNNAIKTKQLGQSIWYDNIDREDLENGKMRELVQQGKVYGVT
                     SNPSIFEKSIGSGNIYDLCLQSMAWAGLDTQQIYFELVKQDIQMTADIFRDVYTYTEK
                     VDGYVSVEIDPTLADDTQKSIQEGKNLWAQINRRNVMIKVPATLAGIPVIRALIAEGI
                     NVNATLIFSIDRYRDVMEAYLLGIEERLKKGLDVSHIHSVASFFVSRIDTAVDKLLDE
                     KIASGSVEANELKGKIAIDNARMAYQEFKQIFSSPRFEALAKHGAQIQRPLWASTGTK
                     NPNYSDCLYVDELIGPNTVNTVPTKTLHAFLDHGKVDQRISYDIGNSIKRLDMLIDLD
                     IDFTSVTDKLEADGVLAFQKAHESLLDVIEKKRRIFAAEIKPIIENLKDAVKYSTDKN
                     FIQRLLENDPTLWTDDASQYEEIRNRLGWLSLPAKQYDLVQEYDQFLEQILQDGFTDV
                     FVLGMGGSSLAPEVLAQSFETYLVTDKRLRLQIIDTTNPIEIKARCETINLEKTLFIV
                     ASKSGGTSETMAAYKYFSNEIAKISPKDFGRQFVAITDPGSPLVELAKKDGFRAVFYA
                     PANVGGRFSAFTPFGLVPASLIGLDLENLLNKAKFSDRLSQKSIALAANPNAILGLAI
                     GSAAKAGRNKLTFIAESFAANLVPWLEQLIAESSGKDGVGILPVENEPELHSYDYPND
                     RLFIYFEVDGSKTEMIDAIRQKGLPVLTFKLSNPYDLAREFYRWEYATAIACAYLRVN
                     AFNQPDVQSNKLVTKAVIEKYKETGQLDEGKAFFCNNQVEVYGEALAGLERCNSLDDV
                     IHLYAQGLEEGAYIAINAFLPRLPEEIEKFQALRSSLLNKYNVATTLGFGPRFLHSTG
                     QLHKGGPGEGVLYLIFTRKNAIDIEIPGEGMSFGTLQLAQALGDFDVLKDLGKNVIRI
                     DLPDNGENK"
     gene            4858..5313
                     /gene="ruvX"
                     /locus_tag="PK791_00335"
     CDS             4858..5313
                     /gene="ruvX"
                     /locus_tag="PK791_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOH91793.1"
                     /translation="MTDIKATGRKFLGVDHGDKRIGLALSDDTLRFARPLRIIEHVSR
                     EDDAKRVIDIAHIEACTDILVGVPYDSDGNVGPRARKVLRFVEMLKSLTTLQVDVWDE
                     SGSTMAFKNLSLQMGQSAKQRRKPSDDRVAALILQDYLDNHYLAKDKND"
     gene            5306..6451
                     /gene="mltG"
                     /locus_tag="PK791_00340"
     CDS             5306..6451
                     /gene="mltG"
                     /locus_tag="PK791_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOH91794.1"
                     /translation="MTKTEKKNAFIFRYLFFSILLIASFLLLACYLTVSKAEKNFGKA
                     DRQLPVVSRLKYAYILNKNNAILTPLVAFPDDIDKKFFISSDETVLEICAKLENEKIV
                     PNGDLACQLLIYYGRDRMIQPGSYTVPSGLNTIEIVQRVGNVAYRDKDLYIFAGWRLE
                     EIANAIDTLGLSFTGDEFLSSALNIPNEIREKYEFLPGDSLEGFILSGQYHLQPKITL
                     IEALELFMDTFESKVVLSGLKAELETQKLSLIESLTMASIIQRETLAVEEMPTMASVF
                     YNRLRLGMRLETDVTVQYAIGWDDNTETWWKPGLTWTDLAINSSYNTYQVNGLPPGPI
                     CSPSLEAIQAVARPAQSDYLFFRAACDNSGRHNFAKTYEEHLNNGCE"
     gene            6459..7205
                     /locus_tag="PK791_00345"
     CDS             6459..7205
                     /locus_tag="PK791_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH91795.1"
                     /translation="MIRLGIDIGGSAIKAALVNLDTGEIVSDTLRAKNNIETKPHDAA
                     VNILRIMRKLDYSGPLGVGFPGRLVKNVLHKAPNLHPEWLNNDLTVFFEENTKSKTVV
                     LNDADAAALAELNFGDPRIKTTTRAMFLTLGTGIGSALILNGQIWNDTEFGQLIFEGK
                     RTMENVGAAAVKRIENLSWKQWGIRLNSVLEKYDFYFSPELFVLGGAISSSLDKFQRY
                     LDFPEEKVIAAKLGNDAGIIGAAMATEKIV"
     gene            complement(7277..8776)
                     /gene="malQ"
                     /locus_tag="PK791_00350"
     CDS             complement(7277..8776)
                     /gene="malQ"
                     /locus_tag="PK791_00350"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOH91796.1"
                     /translation="MIKQRASGVLLHPTSLPGPYGIGDLGPEAYKWIDFLADTGTKYW
                     QILPLGPTGYGDSPYQCFSAFAGNPYLISPDGLVELGLIDEQDLSNLPQFPTENVDFG
                     SVITWKSNLLSEISKRVADEGMPPALQDEYQAFLDDSKIWLDEYALFMAIKESQGNVA
                     WSDWLVPLKFRDENILRETRVRLTKEIFHQKFIQFLFFKQWSDLRQYAHSKDIQIIGD
                     MPFVIAFDSADAWSRPELFLLDEDLQPTLVAGVPPDAFGPKGQLWGNPLYNWPAHDEE
                     NYAWWVKRLEATLKLVDLVRIDHFRGFAAAWHIPFGNEDAVIGEWLPSPGEALFNAFQ
                     AKYPELPIIAEDLGYITPDVVALRENFNLPGMRILQFAFDGDPDNDFLPHHYTINSVC
                     YTGTHDNETAKGWYINAEQKNKNFMHKYLDNNDEDIAWVMMRAAFRSVANLCVVPMQD
                     VLGLDSKSRMNNPGIAAGNWGWRMKPDAINDKLIKRFKDLNFTYSRLND"
     gene            complement(8780..10267)
                     /locus_tag="PK791_00355"
     CDS             complement(8780..10267)
                     /locus_tag="PK791_00355"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOH91797.1"
                     /translation="MKKSINILFAVAEAAPFAKVGGLGDVAGALPEALNKLDEFNFDV
                     RIFMPFHARVHEYDPDRRKIGNFTFSLMSGDSLECELFIAQIGSTKVYLIDNEYINHN
                     SPIYHGDWRLDGLKYVNFSLMVLEASKFINWKPDILHCNDWHTALAINALDQHYANDS
                     FFKDTRTVLSIHNLPYNGWGSQEAMSQLGFSPSTDPDLPDWAKFTPLPMGIAATDLVI
                     AVSPGYSKEIQTPEYGCGLENYLSKHSDKVMGILNGLDIDSYNPETDEVISHSFSSDR
                     LYQRLMNKLHLQSTLGLEVGHQIPLMTMVSRLSHQKGIDLLLDSLEQILHLPWQFVLL
                     GTGDPELEERANNFAKSHPEKFVALLKYDENTARQLYASGDIFTMPSRYEPCGLSQMI
                     AMRYGNIPVASATGGLKNSIFDYSDGLDTATGFLSHQFSVEDFSQTLGKALSLFTDKV
                     IWTQIQRNAMAQDFSWHHSAITYAQAYLNLINPDIPENITLIDEA"
     gene            complement(10275..12257)
                     /locus_tag="PK791_00360"
     CDS             complement(10275..12257)
                     /locus_tag="PK791_00360"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOH91798.1"
                     /translation="MKECNTILKNALILTMDNNYTQYQQGAVAINDGAIVAIGPEDAI
                     LKSWNGKDIIDCQGKVLMPGLINTHTHVPMTLLRGLANDLRLDVWLMGYMMPVEREFV
                     SPEFVRLGTQMGCAEMIRSGVTTFNDMYYFEDEVATATAEIGMRAVVGQTVMKFSAPD
                     AASYDDSLDLSIELIKKWKDHHLIVPAIAPHAVYTCPAEVLEAVVEIAKQYDATVHFH
                     VSETADEVVNMRKQSGMPVVPYIKKLGMLDTKLIAAHCVHVDDGEIRTMRRAGTGIAH
                     NPSSNLKLASGFAPTARMMEMGAKVGIGTDGAASNNDLDMFEEMRLASLIAKPVAGDP
                     TVLPARQVLEMATRIGAEVLHLDHITGSLEVGKRADIIVLDIFPIHNQPHFKRDSENI
                     YAQIVYASKASDVTDVMVDGQWLMRNKTLLTIDEDALIRQAKPVAEQIDAFITSREKS
                     VHSKLLAIGGAAEEESFEVQAKVRISDPDEIIKRLEHPEIEILKRRHYHQYDSYFLFE
                     DSTQGILRYREDEILNKKGDIQNVRGRLTLIGEGSDDVGKQIKQGLLSRSRYFAPATH
                     SLRFYIEYFKPVEMMEIEKDRRRFLVKFKGVEFYINVDTIIKPIEDYFVEIKSRTWSR
                     QDADEKTHLIIYLADVLGLSTQQIINKDYFQLLRNR"
BASE COUNT         3739 a         2356 c         2640 g         3535 t
ORIGIN      
        1 ccatacatcg cttaaagatt gccgcgtggt aaaattttac catcaagcat tataaattat
       61 cggaggtcgc atgaataaga ctgatattga acaagccttg aatacaattc gtttcttttc
      121 agcggatggt gttcaattag ctaattctgg acatacaggt atgccaatgg gcgctgctgc
      181 cctggcttac acgttatatg ttagacaaat gaaccataac cccaaaaatc caaactggat
      241 aaatcgtgac agattcgtac tctctggtgg acatgcatca atgctactgt atacaatcct
      301 gcatttgacg ggttacgata tcagcattga cgatttgaaa caattcagac aatatcagtc
      361 cattacaccg ggacatcccg aatggggtgt tacgccaggt gtagatacca gtactgggcc
      421 actcgggcaa ggtttcgcaa ctggtgttgg tatggcaatc gccgaagctc atttggcagc
      481 gactttcaat aaacctggtt ttgatattgt taaccattac acttatgcca tcgtaaccga
      541 tggtgacatg atggagggta taagttctga agccgcatca ttagctggta ctttaaagct
      601 tggtaaatta atttatctat atgactcaaa cgatatcacc attgaaggca gaacagatat
      661 tgccttaaca gaagatgttg caaaacgctt tgaggcatat aactggcatg ttcaaacagt
      721 tgaagatgga aatgatgttg aggcaatcga taaagcgatt gaggcggcca aacttgaccc
      781 aagaccatct ttgattattt gtaaaacaat tatcggatat ggcttaccta atcgcgctga
      841 tacatcaggt attcatggct caccggcagg ttgggatgaa ttgaatctcg ccaaagaaaa
      901 tgctggcatt cctaccgagc cattgtttta tattactgat gagatacttg cacatttcag
      961 ggaacaaata gcagttggcg ctgaacttca aaaagaatgg aacgaacttt atacatgcta
     1021 taaagagaaa tatcctgacc tggcgaaaga atttgaaaga cgcaatgccc gaatattgcc
     1081 agaaaactgg gatgaaaatc taccagtatt tgaacctgat aaaaagggtt tagcaacacg
     1141 agtagcatcc tggcacacta ttcagaaaat tgcagacgct ttacccgaag ttgtgggggg
     1201 aagtgccgac ctcgcgccaa gcaacaacac ctggatggat gcttatcccg catttggacc
     1261 agaacaacgc gatggaagga atattcatta cggtgtacgt gagattgcta tggcagcaat
     1321 cggaaacggt ttatttgtgc atggcggaat aagacctttt aatgctacat tcctggtctt
     1381 ttcagattat ttacgtggct ccttaagagt tagtgctttg tcaaatattg caaacatatt
     1441 tgttttaaca catgattcga tcggtgttgg agaagatgga ccgacccatc aaccggttga
     1501 aacaattatg agtttaaggc ttatgccaaa cttgaatgtt attcgaccag ccgatgcaaa
     1561 cgaaactgta gaagcctgga aagcggcaat cgagaaaaca gacggaccaa cagcattaat
     1621 tttatcgcgt caaaatatac caacaattga tcgccagaag tatgccagtg cgaaaggatt
     1681 acataagggt gcctatgttc tttataaaag caaagatggc acgcctgatt taattatcat
     1741 tgctgccggc tctgaagtta cattagcact agattgcata tctgcgcttg aagaaaaagg
     1801 tttgaataca cgtgttgttt ccatgccgtg ttgggagctt tttgaagctc aggacaaggt
     1861 atacaaagac gaaattttgc cacccagtgt gaaaaacaga atctcattag aagcaggtca
     1921 aacgctaggt tgggagcgct gggttggaca atatggtgtt agcttgggtg taaccgagtt
     1981 tggtcttagt gctccaggcg atatcatttt caaagaattc ggttttactg ttgaaaatgt
     2041 tatcagtatg attgaaaaga tgatgaagaa gaatcaaggg taatggaaat gaataatgca
     2101 attaaaacaa aacaattagg acagtcaatt tggtatgaca atattgaccg tgaagacctt
     2161 gaaaacggaa agatgcgtga acttgttcaa caaggcaagg tttatggtgt gacttcaaac
     2221 ccaagcattt ttgagaaatc aatcggttca ggcaatatct atgatttatg tctgcaatcc
     2281 atggcatggg caggcttaga cacgcaacaa atatactttg aactggttaa acaagatatt
     2341 caaatgactg cggatatctt cagagacgtt tacacttata ctgaaaaagt tgatggttat
     2401 gtgagtgtgg aaattgaccc aactctcgct gatgataccc aaaagtcaat acaggaaggt
     2461 aaaaatctct gggcgcaaat aaaccgacgc aacgtgatga taaaagtgcc tgcaacgctt
     2521 gcgggaattc ccgtcattcg cgccttgatc gctgaaggca taaatgtaaa tgctacgctc
     2581 attttttcga ttgaccgata ccgagatgtt atggaagcct acttgcttgg tattgaagag
     2641 cgattgaaga aaggtttgga tgtaagtcac attcacagcg tggcgagttt ctttgtttcg
     2701 agaattgata ctgctgtgga taagcttctt gatgaaaaga tagcatcggg atctgttgag
     2761 gcgaatgaat taaaaggaaa aattgccata gataacgcgc gtatggcata tcaggagttt
     2821 aagcagatct ttagcagtcc aaggtttgag gcattggcaa aacatggtgc acaaattcaa
     2881 cgtccgttgt gggcgtccac gggtacaaag aatccgaatt attcagattg tctgtatgtt
     2941 gatgaactga ttgggcccaa cacggttaat actgttccaa ccaagacatt gcatgcattt
     3001 ttggaccacg gcaaggttga ccaacgaatt agctatgata tcggaaattc aattaaacgg
     3061 ctggacatgc ttattgatct ggatattgat tttacaagtg ttaccgataa attagaagct
     3121 gatggtgtgt tggctttcca aaaagcacat gaaagtcttt tggatgttat tgagaagaaa
     3181 aggcgcattt ttgctgcaga gattaaacct ataattgaaa atttaaaaga tgcggtgaaa
     3241 tatagcacgg ataagaactt tatccaacgt cttttggaaa atgacccaac attatggact
     3301 gatgatgcaa gtcaatatga agaaatacgc aaccgtttag gctggctgtc acttccagca
     3361 aagcaatatg atttagtaca ggaatacgat cagtttcttg aacaaattct tcaagatgga
     3421 tttactgatg tctttgtctt aggcatgggt ggttcatcat tagcgccaga agttttggca
     3481 caatcttttg aaacttatct ggtaaccgat aaacgactgc gtttacaaat tatcgacaca
     3541 actaatccga ttgagataaa agcacgatgt gaaacgatta acttagaaaa gacactattt
     3601 attgttgcaa gcaagagtgg tggtaccagt gaaaccatgg cggcctacaa atacttttca
     3661 aacgaaatag ctaaaatctc accaaaagat tttggcagac aatttgttgc cattactgat
     3721 ccgggttcac cgttagttga attagcaaag aaagatggat ttcgagctgt tttttacgct
     3781 cctgcaaatg ttggcggacg cttttctgca ttcacaccat ttggtttggt gccggcaagt
     3841 ttaatcggtt tggatttgga aaacctatta aacaaagcta agttctcaga ccgattaagc
     3901 caaaagtcaa ttgcattggc tgcgaatcca aatgccattt taggcctggc gataggaagt
     3961 gctgccaaag cgggacgcaa caaattgaca tttattgctg aaagttttgc tgctaacctt
     4021 gtaccctggc ttgaacagct catcgctgaa agcagtggca aagatggcgt cggcattttg
     4081 cctgtcgaaa acgaacctga attgcattcg tatgactatc caaatgacag gctattcatc
     4141 tactttgaag ttgatggaag taaaactgaa atgattgatg caattcgcca aaagggatta
     4201 ccagtcttga cctttaaact ttcaaacccc tatgatttgg cccgcgaatt ttaccgttgg
     4261 gaatatgcaa cagcgattgc atgtgcttat ttgagagtga atgcgttcaa tcaacctgat
     4321 gtgcaaagta ataagctggt tactaaggct gtcattgaaa aatataaaga aacaggccag
     4381 cttgacgaag gaaaagcttt tttttgcaat aatcaggttg aagtctatgg cgaagcttta
     4441 gctggtttgg aacgatgcaa ttcactcgat gatgtgattc atttgtatgc ccaaggatta
     4501 gaagaaggcg catacatcgc aataaacgcc tttttgccaa gacttcctga ggaaatagaa
     4561 aagttccaag cgcttcgttc aagtttgctt aacaaatata acgttgccac aaccttgggc
     4621 tttggccctc gattcttaca ttccacagga caattacata aaggcggtcc tggtgaaggt
     4681 gttttgtatc taatctttac cagaaaaaac gcgattgata ttgagatacc tggagaagga
     4741 atgagttttg ggactttaca attagcccag gcacttggcg atttcgacgt attaaaagat
     4801 ttaggaaaga atgttattcg catagatttg ccggataacg gagaaaacaa atagtcaatg
     4861 actgatatca aagcaactgg gcgcaagttc cttggagtag accatgggga caagagaata
     4921 ggacttgcgc tcagtgatga tacattgcgc tttgcaagac cattacgaat tattgaacat
     4981 gtatcacgtg aagatgatgc aaaaagagtg atagatattg ctcatattga agcttgtaca
     5041 gatattcttg taggtgtacc ttatgacagc gatggaaatg taggaccccg ggcacgaaaa
     5101 gttttgcgct ttgtagagat gcttaaatct cttacaacac ttcaggtgga tgtatgggat
     5161 gaaagcggaa gtacaatggc atttaaaaac ttgtctttgc aaatgggaca gtcagcaaaa
     5221 caaagacgaa agccatcaga tgaccgggtt gctgctttaa tacttcagga ctatttagac
     5281 aatcattatc tagcgaaaga taaaaatgac taaaacagag aaaaagaacg cttttatctt
     5341 tagatatctg tttttctcaa ttttgcttat tgcaagtttt cttttacttg cctgttattt
     5401 gacggtatca aaagctgaaa agaattttgg aaaagcggat cgtcaacttc ctgtcgtttc
     5461 gcgattaaaa tatgcgtata ttctaaacaa aaacaacgct atattaactc cgcttgttgc
     5521 ttttcctgat gatattgata aaaagttttt tatcagcagt gatgaaaccg tgttagaaat
     5581 ttgcgccaaa ttagaaaatg aaaagattgt tccgaatggt gatttagctt gtcagttgct
     5641 catatattac gggcgcgaca gaatgattca gccagggtca tatactgttc cgagtggttt
     5701 aaatacgatt gaaattgttc aacgcgttgg gaatgttgcc tatcgcgata aagatttgta
     5761 catctttgcc ggttggcggc ttgaagaaat tgcaaatgcg attgatacgc ttggtttgtc
     5821 gtttacgggg gatgaatttc tgtcatctgc tttaaacatc ccaaatgaaa taagagaaaa
     5881 atatgaattc ttaccaggag attctttaga aggttttata ctttcggggc agtatcatct
     5941 tcaaccaaaa ataaccctta ttgaagcgct agaattgttc atggatactt ttgaatcaaa
     6001 agttgtctta agtggattaa aagcagaact tgaaacacaa aaattaagtt tgattgaaag
     6061 tctaacgatg gcatccatca ttcagcgcga gactttggca gttgaagaaa tgccaacaat
     6121 ggcatcagtc ttttataatc gccttaggct tggtatgcga cttgaaacgg atgtaacggt
     6181 gcaatatgca atcggttggg atgacaacac agaaacctgg tggaaaccag gcctgacttg
     6241 gacggatttg gcaataaatt cgtcttataa tacttatcag gtaaatggat tgccacctgg
     6301 acctatttgc agcccaagct tggaggctat tcaagctgta gcacgacctg cacaaagtga
     6361 ttatttattc tttagggctg catgcgataa ttctggcaga cataattttg caaaaacata
     6421 tgaagaacat cttaacaatg gatgtgaata ggtgaaatat gattagatta ggtattgata
     6481 tcgggggttc cgctatcaag gcggcgttgg taaatcttga tacaggagaa attgtttctg
     6541 atactttaag agcgaagaac aatatagaaa caaagcccca tgatgctgct gttaatattt
     6601 tgcgaataat gcgaaagttg gactatagcg ggccactcgg agttggattt ccgggcaggt
     6661 tagtgaaaaa tgttttacac aaggctccta atttgcatcc agaatggctg aataatgatt
     6721 tgactgtctt ttttgaagaa aacacgaaat caaaaaccgt tgttctgaat gacgcagatg
     6781 ctgctgcgct tgccgaactt aactttggcg atccgcgaat aaaaacgaca acccgtgcaa
     6841 tgtttctaac gctaggcaca ggtattggtt ctgctttgat tttgaatggg caaatttgga
     6901 atgataccga atttggacaa cttattttcg aaggaaaaag gacgatggaa aatgtaggtg
     6961 ctgctgctgt aaagcgtatt gaaaatctaa gctggaaaca atggggaatt cgtctcaata
     7021 gtgttttgga aaaatacgat ttttatttta gtcctgaatt gtttgtgctt ggcggggcga
     7081 ttagttctag tctggataag ttccaaagat atctcgattt tcctgaagaa aaagttatag
     7141 ctgcaaaact tggcaatgat gctggcatta ttggcgcggc aatggcaacc gaaaaaatcg
     7201 tctaaagaaa tagaacaata atagaaaaaa ggcttgcgac aattgacaag cctttttgca
     7261 accaaatttc attttatcaa tcatttaagc gactgtaagt aaagtttaag tccttaaatc
     7321 tttttatcaa tttgtcgttt atggcatcag gtttcatgcg ccaaccccag tttcctgcag
     7381 cgataccagg attattcatg cgggatttgg aatctaagcc taacacgtct tgcatcggca
     7441 ccacacacaa gtttgccacg gaacggaaag cagcacgcat cataacccag gcaatatctt
     7501 cgtcgttgtt atcaaggtac ttgtgcatga agttcttatt cttttgttcg gcgttgatat
     7561 accagccttt ggcggtttcg ttatcatgcg tgcctgtgta acaaacgcta tttatggtgt
     7621 agtgatgggg caggaaatca ttgtctggat caccatcaaa ggcaaattgt aaaatacgca
     7681 tacccggtaa attgaaattt tcacgtaaag ccactacatc gggtgtgatg tacccaagat
     7741 cttcagcaat aatgggtaat tcaggatatt ttgcctgaaa ggcattaaat aaagcttcgc
     7801 cgggagaagg taaccattcg ccaataacag cgtcttcgtt gccaaaagga atatgccagg
     7861 cggcagcgaa acctctaaag tgatcaatac gtactaagtc aacaagtttt aacgttgctt
     7921 ccaaacgttt tacccaccag gcataatttt cttcgtcatg ggcaggccaa ttgtaaagcg
     7981 ggtttcccca aagttgcccc tttgggccaa aagcatcagg tggtacacca gcaactaatg
     8041 taggttgtaa atcctcatcc agcaagaaaa gttctggccg cgaccaggca tcggcactgt
     8101 caaaagcgat gacaaagggc atgtccccga taatctgaat atctttgcta tgagcatatt
     8161 gacgaaggtc agaccattgt ttgaaaaaca aaaactggat aaatttttga tggaatattt
     8221 cttttgttaa tcgaactctt gtttcccgca aaatattttc atcacggaat tttaggggta
     8281 ccagccaatc agaccaggcc acattcccct gactttcttt aatagccata aacaaagcat
     8341 attcgtcaag ccaaattttg ctatcatcga gaaaagcttg atactcatct tgtagggcgg
     8401 gcggcatacc ttcatcagca acacgcttac taatttcaga cagcaaattg cttttccaag
     8461 tgattacact tccaaaatca acattttctg ttgggaattg tggaagatta cttagatctt
     8521 gttcatcaat tagtccaagt tcaactagtc catctggact aatcaaatag ggattacccg
     8581 caaaggccga aaaacattga taaggagaat cgccatatcc tgtgggtcca agtggtaata
     8641 tttgccaata ttttgtgcca gtatctgcta aaaagtcaat ccatttataa gcttctggtc
     8701 ccaaatcacc aatgccatag ggtcctggca agctggttgg gtgtaatagt acgccagagg
     8761 cgcgttgttt aatcatcttt tatgcctcat ctataagtgt tatattttca ggaatatcag
     8821 gatttataag atttagatat gcttgcgcat atgttattgc agaatgatgc caactgaaat
     8881 cttgcgccat cgcatttcgt tggatttgcg tccagattac tttatcggtg aacaaggaca
     8941 atgcttttcc taatgtttga gagaaatctt ctacagaaaa ctgatgtgaa agaaaaccag
     9001 ttgctgtatc caatccatcg gaataatcga aaattgaatt cttaagaccg ccagtggcgc
     9061 tggcaacagg tatattccca taccgcatcg caatcatttg agaaagaccg catggttcat
     9121 atcgggaagg cattgtaaaa atatcgccac ttgcgtatag ctgtcgtgct gtgttttcgt
     9181 catactttag caaagcaacg aatttctcag gatgactttt tgcaaaattg tttgcacgtt
     9241 cttctaattc gggatcacct gtaccaagca ggacaaattg ccagggaaga tgaagaattt
     9301 gttcaagact gtcaaggagt aaatcaatac ctttttggtg acttagccga ctaaccatag
     9361 tcatcaatgg gatctggtga ccaacttcaa gacctaatgt agattgtaaa tgaagtttat
     9421 tcatcaaccg ttgatatagt cgatctgaac taaatgaatg agaaatgacc tcatcagttt
     9481 cagggttgta actatcgata tcaagaccat ttaaaatacc cataacttta tcggaatgct
     9541 ttgaaagata attttccaag ccacaaccat attcaggagt ttgaatttcc tttgaataac
     9601 cgggagagac cgcgatgaca agatctgtag ctgcgatacc catcggtaat ggtgtaaatt
     9661 ttgcccaatc aggtaagtca ggatcagtgc ttgggctaaa accaagttga ctcattgcct
     9721 cttgcgaacc ccaaccattg tatggcaggt tatggattga caaaaccgtg cgggtatcct
     9781 tgaaaaatga gtcattcgca taatgttgat caagcgcatt tatcgccagg gctgtatgcc
     9841 aatcgttaca gtgcaatata tcaggtttcc aattaataaa tttactggct tcaagaacca
     9901 ttagagaaaa attaacgtac ttcaaaccgt ccaaacgcca atcaccgtgg tatatcggag
     9961 aattatgatt gatatattcg ttgtcaatta aataaacttt tgttgaacca atttgggcga
    10021 taaatagttc acattccaat gaatcgccac tcattaacga gaaagtaaag ttgccaatct
    10081 tgcgtcggtc aggatcatat tcatgaaccc gggcgtgaaa tggcataaaa atacgcacat
    10141 caaaattgaa ttcatctaat ttattaaggg cttctggcaa agcacctgcg acatcaccca
    10201 agccaccgac cttggcaaat ggagcagctt ctgcgacagc aaaaagaata ttgatgcttt
    10261 ttttcatata tcacctacct gtttcttaaa agttgaaaat aatccttatt aataatctgt
    10321 tgcgtactca agcccaggac atcagcaaga tatatgatga gatgcgtctt ttcatcagca
    10381 tcttgacgac tccaggttct gctttttatt tcgacgaaat aatcttcaat cggcttaatg
    10441 attgtatcaa cattaatgta aaattccaca cctttgaact ttacaaggaa tctacgtcta
    10501 tctttctcga tttccatcat ttcaaccggt ttgaagtatt caatatagaa tcgcaggcta
    10561 tgtgttgccg gcgcaaagta tcggctacgt gatagcaagc cttgctttat ttgttttccg
    10621 acatcatcac tgccttcacc aattaaggtt agtcggccac gtacgttttg tatatcaccc
    10681 ttcttgttga gaatctcatc ttcacggtaa cgtaatatac cttgagttga atcttcaaac
    10741 aagaaatagg aatcatactg atgataatga cgtctcttga ggatttcaat ttcggggtgt
    10801 tcaaggcgtt tgataatttc atctggatca gaaatgcgca ctttggcttg cacttcaaag
    10861 ctttcttctt cagcagcacc accaattgcc agtagtttgg aatgaactga tttttcccgg
    10921 cttgtgataa atgcgtcaat ttgttcagca acaggtttgg cttgtctgat taacgcatct
    10981 tcgtcaatcg ttaaaagagt tttatttcgc atgagccact ggccatcaac cataacatcg
    11041 gtaacatcgc tggctttgct tgcataaaca atttgtgcat aaatgttttc agaatcacgc
    11101 ttgaaatggg gttggttatg gatcgggaaa atgtcgagaa ctatgatatc tgcacgtttt
    11161 ccgacttcaa gactgcctgt aatatgatca aggtgtaaga cttcagcgcc aattctggta
    11221 gccatttcca aaacttgtcg cgcaggcaga actgttgggt caccagcgac tggtttggcg
    11281 attaaactag ccagacgcat ttcttcgaac atgtctaagt cgttatttga agcggctcca
    11341 tctgtgccaa tgccaacttt tgcgcccatt tccatcattc gcgcggttgg ggcaaagcct
    11401 gatgccagtt ttaagtttga actgggattg tgagcgatac ccgttccagc acgtctcatg
    11461 gtgcgaattt cgccgtcatc aacatggaca cagtgtgctg caataagttt ggtatccaac
    11521 atgcctaatt ttttaatgta gggaactact ggcattccag actgtttacg catattgaca
    11581 acttcatcgg ctgtttctga gacatgaaaa tggacagtag catcatattg ttttgcaatt
    11641 tcgacaactg cttcaagcac ttcggcaggg caggtgtaaa cagcatgggg cgcgatagct
    11701 ggaactatca gatgatgatc tttccatttc tttatgagtt caattgaaag atctaatgaa
    11761 tcatcataag aagccgcatc aggggcagaa aatttcatta ctgtttgtcc aaccacagcg
    11821 cgcataccaa tctccgcggt ggcagttgca acttcgtctt cgaaataata catgtcatta
    11881 aaggtagtaa caccagatct aatcatttcc gcgcaaccca tttgtgttcc taaccgcaca
    11941 aactcaggcg agacaaattc gcgttcaacg ggcatcatgt aacccatcag ccacacatca
    12001 agccgaaggt catttgccaa accacgtaaa agagtcatcg ggacatgggt atgggtatta
    12061 attaatccag gcatgagcac cttgccctgg caatcgatta tgtctttccc attccagctc
    12121 tttaagatgg catcttctgg accaatagcg acaatagcac cgtcgttaat tgcaacggca
    12181 ccttgctggt attgggtgta gttgttatcc atagtaagta tcaaagcgtt ttttaggata
    12241 gtgttacatt ctttcataag gctccttttg
//
LOCUS       DAOSFC010000007        11646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_4650_length_11646_cov_8.039859, whole genome shotgun sequence.
ACCESSION   DAOSFC010000007 DAOSFC010000000
VERSION     DAOSFC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4650_length_11646_cov_8.039859"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PK791_00365"
     CDS             complement(<1..949)
                     /locus_tag="PK791_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH91799.1"
                     /translation="MNELPWLLTLLGVAVVFDLLTSAARAAFSQAQLPNLLTYFEKEP
                     HKAERTLEVSGKLGLRTSLRLAIAFAHLFLGALTVLVTHYFWPDLHFAWVLLIALGVL
                     FVISIFEYFISRKVLDTPEKSAYSWHRFADFLYFVFKPMTIVMTGLLGPHAEINVPAV
                     TDETLRDWVSLEQAEGMLEKGEREMIYSIFQFSETLTKEIMVPRMDVLALDIESTISH
                     ARQEFINAGHSRVPVYEDVIDNIVGLLYAKDLLAITDDDATIASKRKLLRTAYFVPET
                     KKVDELLTEMQSRGVHLALVVDEYGGFAGVVTLEDIVEEI"
     gene            complement(951..2306)
                     /gene="glmU"
                     /locus_tag="PK791_00370"
     CDS             complement(951..2306)
                     /gene="glmU"
                     /locus_tag="PK791_00370"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOH91800.1"
                     /translation="MTIASIMLAAGHGTRMKSTLPKVLHPILGKPLVLHALSAVENVV
                     DIPPVVVIGHGAEDVKAAIQAGVKQDVQFAIQEKQLGTGHAVMCAQSLLQGKSEKVII
                     TFADMPLLTQASIEHIIQLQQESQSVMVITTVMSEEPRGFGRVIRDSDGSVQAIVEEV
                     DCTPEQLLVREKNTSAYCVDAKWLWQSLEKLQPSAKGEYYITDLVAMAVAEGLRVSAY
                     VLEDRSEGLGINDRVDLADTERAMRMRVNRDWMRKGVTFIDPWTTTVHLEVELAQDVV
                     LQPNTMLMGKTKIAKNSVIGPNTTLIDTLVGEDCHIQYSVAESAQVGNHVTIGPFARL
                     RKGAVLQDHVHIGNFGEVKDSVLGEGTKMGHFSYIGNAEIGKDVNIGAGTITCNYDGK
                     TKHLTEIGDSAFIGSDTMLVAPLKIGKNATTGAGSVVTRDVPDNTIVYGTPAKPKERK
                     D"
     gene            complement(2418..2488)
                     /locus_tag="PK791_00375"
     tRNA            complement(2418..2488)
                     /locus_tag="PK791_00375"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2454..2456),aa:Gln,seq:ttg)
     gene            complement(2560..2772)
                     /locus_tag="PK791_00380"
     CDS             complement(2560..2772)
                     /locus_tag="PK791_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91801.1"
                     /translation="MFRISSKTMIIIGGVLLTLSVIIPALRLAEILPALIWIDFLTYA
                     LLVSGLFLGMMGIFTYAKEVRDRNRE"
     gene            complement(2802..3845)
                     /locus_tag="PK791_00385"
     CDS             complement(2802..3845)
                     /locus_tag="PK791_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH91802.1"
                     /translation="MTDQIDEKTIEQNFEQDEDFVLDIRGLKVYFELRKRGFQHAGFV
                     HAVDNVDLRLRRGETIAIVGESGCGKTSLMKTILRLNTPTGGEIEFDGEIINDLKSDA
                     LKNVRRKIGYVQQDPYGALAPFMNVRTILEEPLIVNGVKDKQERFDRVVKVLEEVRMT
                     PVEDFLEKFPHQLSGGQQQRIVIARAMILDPILLVADEPVSMLDASVRVEILKLLRSL
                     QEEHNLAVIYITHDLATVRYFSEYIYVMYAGQIIEKAPESDLINHTAHPYTHALLAAT
                     SDPDPKNVDEFQDVPPGEPPSLVKPPEGCRFHPRCPHMIADLCDKEVPLEFEIAEKHL
                     VHCWLFRDKAVAK"
     gene            complement(3847..4923)
                     /locus_tag="PK791_00390"
     CDS             complement(3847..4923)
                     /locus_tag="PK791_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH91803.1"
                     /translation="MAKQKTKDIKYDAIAAAKEKAQLMSQMSETTHKTGPVEHSLLLS
                     IKDLKLHFETVRGPVQAVDGVNFDLVTNKAVVVVGESGCGKSSLAKAILRLLPRNVAH
                     YSGEVWLRDVDTMQLSDDDYREEIRWVKISMVPQAAMNSLNPVLRVGDQVAEPMLVHY
                     GMPKEEAMQQAAIMFRNVGVPVDFLTRYPFELSGGMRQRVAIAMALVTGPDLVILDEP
                     TSALDVLTQANIFNLLKRIKANLDTSFILITHDISTSSELADIVAVMYAGKIIEVSES
                     HRFFSKPRHPYARALMASVPRLREREEPHFIEGKPPSLINPPTGCRFKERCPKRMEKC
                     EEEPPVTRFGDDWVACWLYEGQGD"
     gene            complement(4925..6301)
                     /locus_tag="PK791_00395"
     CDS             complement(4925..6301)
                     /locus_tag="PK791_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH91804.1"
                     /translation="MKKFKDTFQDIVRYPSAIVGLLIVLALIVFSIYTMITIPYKEAI
                     YLWKGTEEVVYKNPRNAQPTWSNWFRKDKLPESIDVYAGVGEEFSKEVTPREKGGSQI
                     EFNYELEYNYNELPQDIFMYFTSNYTDRNPFIVITMITPSGEEIKVFDGGIARATNVR
                     FGQEEKLMNRLTRLHGREPETPLKNVIEGLFTKVGVYPPVVDKGTYTINVKATTFEEG
                     SDVDAELVMHGTVFGLFGTDHMRRDLKIAIMWGAPIALAFGLTAALGTALISMFIAAL
                     GTWFGGWLDELIQRITEVNMVIPYLSILIMVGTFYSRSIWVLLAVTILLNIFSGAIKS
                     RRAIFLQVRESTYIEAAKAYGASDYRIIIRYMIPRVIPMIIPGLVSAVPAYVFLEASL
                     AILGLGDPTVPTWGKIINDARTYGALYQGMFYWILEPAVFLMITGLGFAMLGFALDRI
                     FNPRLRGM"
     gene            complement(6303..7325)
                     /locus_tag="PK791_00400"
     CDS             complement(6303..7325)
                     /locus_tag="PK791_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH91805.1"
                     /translation="MQIITIIIGVYITILVANMGGYVDTILKASIIEEVNMEVSAMVS
                     EKDIPREMREQMVRDLVEERERLAGLDQPFITRSFHHLWNGLTLNFGRARFMVSDHGS
                     RMVRNIILERLPATLLLMGTSQLLLFFVTLSIALAVSRHYGSFWDKLIVAMSPTSSIP
                     PWFYGIFLILIFAAWLKVLPFSGMVDAPPPPTKLGYFLNVLKHLILPAGSLFISSFFI
                     NTYSWRTFFLIYSSEDYVDMAKAKGLGDRAIERRYILRPTLPSIITSFALLMVGIWTG
                     ATLTETIFNWPGLGTTIYTAISKYDTAVIVGTTIIQAYMLGITVFFLDFLYAIVDPRV
                     KLGGGK"
     gene            complement(7516..10125)
                     /locus_tag="PK791_00405"
     CDS             complement(7516..10125)
                     /locus_tag="PK791_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH91806.1"
                     /translation="MSKFARILSLLLVTVLLGGMLIACEQKPVTPEPPVDEVPVEEPV
                     EEPVEEPVEEEPVEEEPVEEPVEEEPEPEPTTRVGGWLDEIVVSIIDAPSVIVQMEAD
                     AVDVYVNGFGTNDLDQLNDSGLPYTTSNGTYYTLQFNPAEFNEGLNVFRNRKIREAFN
                     YLIDRNYINQEFYAGGALPKWFAIMTNFGDYALLADTARRLETKYAYNFEKAKEIIDA
                     EMEAAGYPLTDGKYMNADGEQYEVIFLVRNDGDGTRKAWGTYLTGQMEKLGFAVNFKE
                     GTGGDLGPIWIAGDPKAGEWHMYTGGWGASVLNRDQSNIFQEMYLPTSAQGLPVFMEN
                     DSDPEFQELGDKLYNREFTTMEQRAEMMARALELSLEDSLQVMIVDSKRFVPHQEGLV
                     VTTDLAAGVESAQITPFTLRWEGQEGGTVRWATQPFLFNGPWNPVRGSNTTSDQGAVR
                     MTVGYATTYDPYTGLLNAFWVDKAEVTVQTGLPVQKTLDWLTLDFADEIVVPGDAWAD
                     WDAETQTFITVDEKSGGEPVTATAKAVVYFREDLFDVVKWHDGSNLEMADIMMSYIMG
                     FDRAKEESAIYDKQPVGAFNVFMQTFKGFKIVSTDPLIMEGYQDTFSADAELLVPIGY
                     TWINNFQGEGSFASYAMMNSAEAAHELAYTESKALELEVEQTSTIGGPSLEILTKHLD
                     QLIADGTIPYEPTLGQYITKEQAIARYQALKAHFEKYGNYHQGVGPYFLKSVDLNGKS
                     LVMAHWDQYPDLADRWSMYSAPKISDVEIDGPGQVVGGEEATFDIYVTFQGKPYELAD
                     IKQVKFLVYDAENNVVIISAAEAVEDGHFVITFSGEDTAKFGDGATKLEVAVISIAVA
                     QPTFETIEFITAQ"
     gene            complement(10348..10896)
                     /locus_tag="PK791_00410"
     CDS             complement(10348..10896)
                     /locus_tag="PK791_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91807.1"
                     /translation="MSRKKYINAYETVYEYDEDGRETRRVNYRGDIYEIVTDEVNIKK
                     YKVTQIIIVVLVFLIQLALGFINNAGGRILYVSLPYLIAFLPMAYWFIAATRLPSEKK
                     ELRLEIVDLGFKRSKVMSLLIAIFSGIAILGAIVFMIFFSEGNILKDLLFLLGQVIVF
                     TLALFAHLQSRKIIIRKIETDE"
     gene            complement(10961..>11646)
                     /locus_tag="PK791_00415"
     CDS             complement(10961..>11646)
                     /locus_tag="PK791_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91808.1"
                     /translation="AIAGCLATFDIATSIFNRDWIAHSWQLAMDTQPFVPPVVWTDAK
                     GRLTDLGWVLQEGSDGVRVADGVPNVEDGTPFSINLLTGQSAQSQSMANLIVQNLADC
                     GIAVTHTSLPAEELYAPGPDGVLFGRQFDMALVNWGKLPFPLCELYTSGQIPGPSTHW
                     IGTNIAGLNDNAFNYGCMNGNSDEMMIQYVPAVPLMPQIRVWLASPDLDLTELIRFDL
                     LSELKPVVP"
BASE COUNT         3168 a         2773 c         2601 g         3104 t
ORIGIN      
        1 cgatttcttc aacgatatct tctagcgtta ccacccccgc gaaaccgcca tattcatcaa
       61 ccacaagcgc taaatgcacg ccccgcgatt gcatctctgt gagaagttca tccacctttt
      121 tggtttcagg cacaaaatag gcggtacgta aaagcttgcg ttttgacgcg atggttgcgt
      181 catcatcagt gattgccagg agatccttgg cgtaaagcaa accaacaata ttgtcgatga
      241 cgtcttcata gactggcaca cgggaatgac cggcattgat aaattcctgg cgcgcgtggc
      301 ttatggtcga ttcgatatcc agggctaaaa cgtccatacg gggtaccata atctctttgg
      361 taagcgtttc gctaaactgg aaaatggaat aaatcatttc gcgctcgcct ttttcaagca
      421 tgccttcagc ttgttccaaa gaaacccaat cgcgtaaggt ctcatcggtc acagccggta
      481 catttatctc cgcatgtggc ccaagcaaac cagtcatcac gatggtcatc ggcttaaaca
      541 caaaatacag gaaatcagca aagcgatgcc agctataagc ggatttttca ggcgtatcta
      601 atactttgcg cgagataaag tattcaaaaa tgcttatgac aaacaataca cccagggcaa
      661 tcagaagcac ccacgcaaaa tgcaagtccg gccagaaata gtgggttact aataccgtta
      721 aagcgccaag gaaaagatgg gcaaaagcga ttgccaggcg caacgaagtg cgcaggccaa
      781 gttttccgga aacttcaagc gttctttccg ctttgtgagg ttctttctca aagtatgtca
      841 atagattagg caattgcgcc tgcgaaaagg cggctctggc ggcggaagtc aataaatcga
      901 atacgactgc cacccccaag agtgtcaata gccagggaag ttcgttcacg ttagtccttt
      961 ctctctttcg gtttcgcagg cgtgccatac acaatcgtat tatctggcac atcgcgagtt
     1021 accacagatc cagcgccggt tgtggcattt tttccaattt tcagcggagc aacaagcatc
     1081 gtgtcactgc caatgaaggc agaatcgccg atttcggtaa gatgcttggt ttttccgtca
     1141 taattacagg taattgtgcc cgcgccgatg ttaacatcct tgccaatttc ggcattacca
     1201 atataggaga agtgtcccat tttggtgcct tcaccaagca cagagtcctt cacctcgccg
     1261 aaattaccga tatgcacatg gtcctgcaaa acagcaccct tgcgcaaacg ggcaaatggg
     1321 ccaatcgtga catgattgcc aacttgcgcc gattctgcca cactatattg aatatggcag
     1381 tcttcgccaa ccagcgtatc aatcaaagtc gtatttggtc ctatgactga gtttttggca
     1441 atttttgttt tacccatcag catcgtattt ggctgaagca caacatcctg cgccagttca
     1501 acctcaaggt gcacagtcgt tgtccagggg tcaataaagg taacaccttt acgcatccaa
     1561 tcgcggttaa cccgcatacg catcgcgcgc tccgtatcag ccaggtcaac gcggtcattt
     1621 atacccagac cttcgctgcg atcctcaagc acatacgcgc tcacccgcag tccttcggca
     1681 accgccatgg cgaccaaatc agtaatgtaa tactcacctt tggcggaagg ttgtagcttt
     1741 tccagggatt gccaaagcca tttggcgtcc acacaatacg cgctggtatt cttttccctg
     1801 accaaaagtt gctcaggcgt gcagtcgact tcttcaacaa ttgcctgcac ggaaccgtcg
     1861 ctatcgcgaa tgacgcgccc aaatccgcgg ggttcttcag acatgacggt cgtaataacc
     1921 atcacgcttt ggctttcctg ttgcagctga atgatatgtt caatactggc ctgtgttaaa
     1981 agtggcatat ccgcgaaggt aatgatgact ttttcagact tgccctgcaa gaggctttgc
     2041 gcgcacatca ccgcatggcc agtaccaagc tgtttttcct ggatggcaaa ttgcacatct
     2101 tgttttacac cagcttgtat cgcagctttg acatcttctg cgccatgtcc aatcacaaca
     2161 actggcggaa tatcaaccac attttctacc gcgcttaaag cgtgtaaaac caaaggcttg
     2221 cccaggatag gatgtaatac tttaggtaat gttgatttca tgcgtgtgcc atgaccagca
     2281 gcaagcataa tagaagctat tgtcattgta ttctccaaat ggaacgacga caagcaggaa
     2341 ccggtttact cacgctgctt gtcgtgtaaa aagaatgata tggatcgtca ggttcagttt
     2401 ccacaataat aactaggctg gggtacatgg attcgaacca cgacaaacgg attcaaagtc
     2461 cggtgtgcta ccattacaca ataccccagg gacgattatt ttagcacaat ttccagaata
     2521 tgcaaatgat atctcaagtt tggattattg ttttgcgcgt tattctctgt tacggtcgcg
     2581 cacttccttg gcataggtaa agatacccat catgccaaga aacaagccac taactaaaag
     2641 ggcgtatgtt aagaaatcaa tccagattaa ggctggcagt atttctgcca gccttagtgc
     2701 tggaatgatg acgctaagtg tgagcaaaac gccaccaata ataatcatcg tcttagaact
     2761 gattctaaac aaatccttgt cccttaacaa ttgctatgga tttatttagc cacagccttg
     2821 tctctaaata accaacaatg caccaaatgc ttttcggcta tttcgaactc tagcggtact
     2881 tctttgtcgc acaaatcagc tatcatgtgt gggcaacgcg gatggaaccg acaaccttct
     2941 ggcggtttta ccaggcttgg gggttctcct ggtggaacgt cctggaattc atcaacgttt
     3001 ttcgggtcgg ggtcagatgt agccgccaaa agcgcatgcg tgtagggatg cgcggtgtgg
     3061 ttaattaggt ctgactccgg tgccttttca atgatttgcc cagcgtacat aacatagatg
     3121 tactcagaga agtaacgtac ggttgccagg tcgtgggtga tatagataac cgctaagttg
     3181 tgttcttcct gcagcgaacg caaaagtttg agaatctcta cccgtacaga agcatccagc
     3241 attgagaccg gttcgtctgc aaccaacaaa atcggatcca aaatcatcgc gcgcgcaata
     3301 acgatacgct gttgttgacc gccactaagc tgatggggga acttttcaag gaagtcttcc
     3361 acaggtgtca tgcgcacttc ttcaagcact tttacgacac ggtcgaagcg ttcctgtttg
     3421 tctttaacac cattaacgat taatggctct tcaagaatag tacggacatt catgaatggt
     3481 gccaaggctc cataaggatc ctgttgcaca taaccaatct tgcgacgcac attcttgaga
     3541 gcatccgatt ttaggtcgtt gatgatttcg ccgtcaaatt caatttcgcc accagttggt
     3601 gtgttgagac gcaaaatagt cttcatcaaa cttgtcttgc cacaacctga ttcgcctaca
     3661 atcgctatcg tttcaccacg tcttagcctg agatcgacat tatcgacagc atgtacaaaa
     3721 ccagcatgtt gaaaaccgcg ttttctaagt tcaaaataga cttttaatcc gcggatatcc
     3781 aaaacaaagt cttcatcttg ctcaaagttc tgttcaattg ttttttcatc aatttgatct
     3841 gtcatgctaa tcaccttgtc cttcatagag ccaacaagca acccagtcat caccaaagcg
     3901 agtaacaggt ggttcttctt cacacttttc cattcgcttt ggacagcgtt ctttaaaccg
     3961 gcaacctgta ggtgggttga taagtgatgg cggttttcct tcaataaaat gaggttcttc
     4021 gcgttcccgc aatcgtggca cactagccat caaagctctt gcatagggat ggcggggttt
     4081 gctgaaaaag cgatgggact cgctaacctc aataatcttg cctgcgtaca taactgccac
     4141 gatgtcggca agttctgaag acgtagagat gtcgtgggta atcaagataa aactggtatc
     4201 caggttagct ttgatgcgct taagcaggtt aaagatatta gcctgggtca atacatcaag
     4261 tgccgaagtg ggttcatcaa ggataaccag gtcaggaccg gtaaccagtg ccattgcgat
     4321 agcaacgcgc tggcgcatac cacctgaaag ctcaaatgga taacgggtta agaagtcaac
     4381 cggcacgccc acattgcgga acatgattgc cgcttgttgc atagcttcct ctttgggcat
     4441 accataatgc accagcatag gctcagcaac ctgatcgcct acgcgcaaca ctggattgag
     4501 tgagttcatg gcggcctgtg gcaccatcga aattttgacc catctgattt cttcgcgata
     4561 gtcatcatca ctcagttgca tcgtgtcaac atcacgcaac catacttcac cggaataatg
     4621 agccacgttg cgtggcaaca agcgcaaaat ggctttggca agtgaactct ttccacagcc
     4681 tgattcgcca acaacgacca cagctttgtt ggttaccaaa tcaaaattga cgccatcaac
     4741 tgcctgtact ggaccgcgaa cggtttcaaa gtgaagttta aggtctttga tacttaagag
     4801 caaactgtgt tctactgggc ctgtcttatg agtagtttca ctcatttggc tcatcaactg
     4861 agccttttcc ttagcagcag caattgcatc atacttgatg tctttggttt tttgttttgc
     4921 cattttacat acctctcaat cttgggttga agattcggtc aagcgcaaag cccaacatcg
     4981 cgaacccaag cccagtaatc atcaagaata ctgcaggctc cagaatccaa tagaacatac
     5041 cttgatacag cgcaccatat gtgcgggcat cgttgatgat cttaccccag gtaggtacgg
     5101 taggatcacc cagacctaaa atcgccaaag atgcttcaag gaagacatac gcaggtacag
     5161 ctgataccag acccggaatg atcataggga taacacgggg aatcatgtat cggatgataa
     5221 tgcggtaatc gctggctccg tatgctttag ctgcctcaat gtaggtagac tctcgaactt
     5281 gcaggaagat tgccctgcgt gatttaatcg cccctgagaa tatgttcaaa agaatcgtca
     5341 cagccaaaag cacccaaata cttcgcgagt agaaggtacc caccataatc agaatagaca
     5401 ggtagggaat aaccatattg acttcggtga ttctttgaat gagttcatcc agccaaccgc
     5461 caaaccatgt gccaagcgcg gcaatgaaca tagaaatcag tgctgtaccc aaagcagcag
     5521 tgagaccgaa agccaaggca ataggcgcac cccacatgat ggcgatttta agatctctgc
     5581 gcatgtgatc ggtaccaaaa agcccaaata ctgtgccatg catcaccaat tcagcatcaa
     5641 catccgaccc ttcttcaaag gtggtcgcct tgacattaat cgtgtatgtg cctttgtcta
     5701 caactggcgg ataaacaccc actttggtaa aaagaccttc aataacattc ttcagaggtg
     5761 tttctggctc ccgaccatgc aagcgggtca aacggttcat gagtttttct tcctgaccaa
     5821 atctcacatt tgtggctcgg gcaataccgc catcaaatac ctttatctct tcacctgaag
     5881 gtgtaatcat cgtgatgacg ataaagggat ttctatccgt atagttcgat gtaaagtaca
     5941 taaaaatatc ttggggcagt tcgttatagt tgtattccag ttcgtaatta aactcaattt
     6001 gactgccacc tttttcacgt ggcgtgactt ctttcgaaaa ctcttcgcct acgccggcat
     6061 atacatcaat ggattcagga agtttgtctt ttctaaacca attactccag gttggttggg
     6121 cattcctggg attcttgtag acaacttctt cagtaccctt ccacaaatag attgcttctt
     6181 tgtaggggat agtaatcatc gtataaatag aaaatacgat taacgctaag actatcaaca
     6241 atcctacaat cgcggaagga tatctgacaa tatcctggaa tgtgtcttta aattttttca
     6301 tcttatttcc ctccgcccaa cttaactctg gggtcaacaa tggcatacag gaagtccagg
     6361 aagaacactg ttatacccaa catataagcc tgaataatcg ttgtgccaac aattaccgcg
     6421 gtgtcatact tgctaatggc ggtgtatatc gttgttccaa gccccggcca gttaaaaatc
     6481 gtttccgtca atgtggcacc agtccaaata ccaaccatca gcaaagcaaa gctggtaatg
     6541 atactgggca aggttgggcg caggatatag cgtcgttcaa tcgcgcggtc acccagacct
     6601 tttgccttag ccatatcaac atagtcttcg ctggagtaga ttaagaagaa tgttcgccag
     6661 gaataggtgt ttataaagaa actcgatata aacagcgatc ctgcaggaag aataagatgt
     6721 tttagaacat tcaggaaata tccaagcttt gttggtggtg gaggcgcatc taccatacca
     6781 ctaaaaggca aaacctttaa ccatgcggcg aaaataagga taaggaatat gccataaaac
     6841 catggcggaa tggaagatgt cggcgacatt gccacaatta gcttatccca aaaactacca
     6901 taatgtcttg acaccgccag cgcgatacta agcgtgacaa aaaacaacag cagttgcgac
     6961 gtgcccatta agagcaatgt ggcaggaagt cgctctaaaa tgatgtttcg aaccatcctg
     7021 gatccatggt cactcaccat aaaacgtgcc cggccaaaat tgagcgttaa accattccaa
     7081 agatgatgaa atgagcgggt gataaaaggc tggtctaaac ctgccaatct ttcgcgctct
     7141 tcaacaaggt ctctcaccat ttgctcacgc atttcgcgcg gtatatcctt ttcacttacc
     7201 atagctgata cttccatatt aacttcctca ataattgagg cttttagaat agtatcaaca
     7261 taaccgccca tgttggcaac aagaatagtg atgtaaacac caatgatgat agtgattatc
     7321 tgcaaaacta aacgaataga aaagaatcgc gccaagcgct tagctgtttc tcgtgttgct
     7381 ctctttgcgt attctattgg agtttcctgt gaattcgttt ctatagacat actgggcaac
     7441 ctccctttct tttttaaata aaaggggcaa gtttttgtaa acctgcccct catgggagct
     7501 acttagccac tcagtttact gggcggtaat gaactctatc gtttcgaaag ttggctgggc
     7561 aactgcgatt gagattactg caacctcaag cttggtggca ccatcaccaa atttcgcggt
     7621 gtcttcgcca ctgaaagtga taacgaagtg gccatcttca actgcttcag cagcgctgat
     7681 gataacaacg ttgttttcag cgtcgtaaac aaggaactta acttgtttga tatcagccag
     7741 ttcgtagggt ttgccctgga aggtgacata gatatcaaat gtggcttctt caccaccgac
     7801 aacctggccg ggaccatcaa tctcgacatc ggaaatcttt ggtgcagaat acattgacca
     7861 gcggtcagca agatcaggat attgatccca gtgagccatg accagtgatt taccattcag
     7921 atcaacagac ttgaggaagt aaggaccaac gccctgatgg tagttgccat acttttcaaa
     7981 gtgagctttc agtgcctggt agcgggcaat ggcttgttct ttggtgatgt attgacccaa
     8041 agtgggttcg taaggaatgg ttccatcagc aatgagttga tcaagatgct tggtcaaaat
     8101 ctcaaggctt ggtccaccaa tggtgctggt ttgttcaact tcgagctcaa gagctttgga
     8161 ttcggtgtag gcaagttcat gtgcagcttc tgcagagttc atcattgcat aggatgcgaa
     8221 tgagccttca ccctggaagt tgttaatcca ggtgtagcca atgggaacaa gcaattcagc
     8281 gtcagcgctg aaggtatctt gataaccttc catgatcaag ggatcagtgg aaacaatctt
     8341 gaagcctttg aaggtttgca taaagacatt gaaggcgcca actggttgtt tgtcatagat
     8401 agcagattct tctttggctc ggtcgaagcc catgatgtat gacatcatga tgtcggccat
     8461 ttccaggttg ctgccatcgt gccatttgac gacgtcaaag agatcttcgc ggaagtaaac
     8521 aacggctttc gctgttgcgg taacaggttc gccaccactc ttttcatcaa cagtgatgaa
     8581 ggtttgagtt tcggcatccc agtcagccca ggcatcgcca ggtacaacga tttcatctgc
     8641 gaaatcaagg gtgagccaat ccaaagtttt ctggacaggc aagccagttt gaacagtgac
     8701 ttcggctttg tcaacccaga aggcgttcaa aagaccagtg taaggatcat aggttgtggc
     8761 atagccaacg gtcatgcgca cggcaccctg gtcagaagtg gtgtttgaac cgcggacagg
     8821 gttccatgga ccattgaaca agaagggttg ggttgcccag cgcactgttc caccttcttg
     8881 accttcccag cggagggtga agggggtgat ttgagcgctt tcaacaccag cagccaagtc
     8941 ggttgtaaca acgagacctt cctggtgagg aacgaagcgt ttgctgtcaa caatcatgac
     9001 ctgcagggag tcttcaagtg agagctcaag agcgcgggcc atcatttcgg cacgttgttc
     9061 catagtggtg aattcgcggt tgtagagttt gtcgcctaat tcctggaatt cgggatcgga
     9121 atcattttcc atgaagacag gcaagccttg tgcggaggtg ggcaggtaca tttcctggaa
     9181 gatgttggat tggtcacggt tcagaaccga agcaccccaa ccaccagtgt acatgtgcca
     9241 ttcgccagct ttgggatcgc cagcaatcca gatgggacca aggtcgccac cggtaccttc
     9301 tttgaagtta acggcgaaac cgagtttttc catttggccg gtaaggtaag taccccaggc
     9361 tttgcgggtt ccatcaccat cgttgcggac caggaagatg acttcatatt gttcgccatc
     9421 agcgttcata tatttgccat cagtcaaagg atagccagcg gcttccattt ccgcatcaat
     9481 gatttctttg gctttctcaa agttgtatgc gtatttggtt tcaaggcggc gagcggtatc
     9541 agccaggaga gcataatctc caaagttggt cataatagca aaccatttgg gcagggcacc
     9601 accagcgtag aactcttggt tgatgtagtt gcggtcaatc aaatagttga atgcttcgcg
     9661 aatcttgcga ttgcggaaga cgttgagacc ttcgttaaat tcagcagggt tgaattgaag
     9721 ggtgtaatag gtaccgtttg aagtggtata gggtaagcca gagtcattca gctgatcaag
     9781 gtcattggta ccgaaaccgt tgacgtaaac atccacggcg tcagcttcca tctgcacaat
     9841 aaccgatgga gcatcgataa tagaaaccac gatttcgtct aaccaaccac caacacgggt
     9901 agtgggctcg ggctcgggtt cttcttcgac gggttcctcg acgggctctt cctctacagg
     9961 ttcttcctct acgggttctt cgacgggttc ctcgacgggt tcctcaacag gaacttcatc
    10021 gacggggggt tctggtgtca caggtttttg ttcgcaagca ataagcatgc caccaagtaa
    10081 aactgtgacg agcagaagtg ataagattcg tgcaaattta ctcatatttc tccttttctc
    10141 cttttgggtt tgatttatta taatcggcaa gtctaaaatc tgtcaaccaa ccaactgcct
    10201 taaataataa acggctttta atgcttatta tttgagtttt atccgggaat aattcttttt
    10261 caggtacgtt ttgacgagca gtgaagtcgt ttatatatta tcaatgacaa tttttatgga
    10321 ttttaataaa agatgatttt tcagaatcta ctcatcagtc tcgattttgc gaataataat
    10381 ttttcggctt tgcaagtgcg caaaaagtgc cagtgtaaaa acaataacct gccccaaaag
    10441 gaagagaagg tctttgagaa tgtttccttc gctaaaaaat atcataaaca cgatcgcgcc
    10501 cagaatcgct atgcccgaaa agatagctat caacagactc attaccttgc tgcgtttaaa
    10561 tccaaggtca acaatctcca gccttaattc tttcttttct gatggcaagc gcgtagcagc
    10621 gataaaccaa taggccatcg gcagaaaagc gataagataa ggcaggctta catatagtat
    10681 gcgtccgcca gcgttgttga tgaaacccaa tgccagttga atgaggaaaa caagcacgac
    10741 aataatgatt tgcgtcactt tgtacttttt aatattgact tcgtctgtta cgatttcgta
    10801 aatatctcca cgatagttca cacgacgtgt ctcgcggcca tcttcatcgt attcataaac
    10861 tgtttcgtaa gcgttgatgt attttttcct ggacatattc tctcaatttc ttttcctggc
    10921 aagctttggc attaagttta atcgatatga ttgactttcc ttatgggacc actggtttca
    10981 gctctgataa aaggtcaaaa cgaataagtt ccgttaagtc aaggtcaggc gaagccaacc
    11041 atacacgtat ttgtggcatc aaaggcaccg ctgggacgta ctgaatcatc atttcatctg
    11101 aattcccgtt catgcagcca tagttaaagg cattatcatt taacccggca atgttggtgc
    11161 caatccaatg cgtgcttgga cctggtattt gcccactggt gtaaagttcg catagcggaa
    11221 aaggcaactt gccccaatta acaagcgcca tgtcaaactg gcgtccaaac agcactccgt
    11281 caggtccggg cgcgtaaagc tcttcggcag gcaagctggt atgcgttacc gcgatgccac
    11341 aatctgccag gttttgcacg atgaggttcg ccatggactg actttgcgcg ctttgcccag
    11401 tcaaaaggtt tatactgaaa ggcgtcccgt cttcaacatt tggtacacca tctgccaccc
    11461 gtacgccatc agaaccttcc tgaagcaccc agcctaaatc cgtcaatctg cctttagcgt
    11521 cagtccagac aaccggtggg acgaatggct gtgtatccat agctaattgc cagctatggg
    11581 caatccagtc acggttaaat atacttgtag caatatcgaa tgttgccaga cagccggcga
    11641 tagctt
//
LOCUS       DAOSFC010000008        11630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_4656_length_11630_cov_5.150929, whole genome shotgun sequence.
ACCESSION   DAOSFC010000008 DAOSFC010000000
VERSION     DAOSFC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4656_length_11630_cov_5.150929"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PK791_00420"
     CDS             complement(<1..370)
                     /locus_tag="PK791_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH91809.1"
                     /translation="MENGLSVLLIEGKRRLKNNFAQELSDKGFDVATVTSGATALETI
                     PDLMPNVVVINAMSLRTNGLRITNWIRTAWPDLPIILIIAEDEAITNAPQANVLLRLP
                     FTVQKLINRLRLFNEVKHKHI"
     gene            629..1267
                     /locus_tag="PK791_00425"
     CDS             629..1267
                     /locus_tag="PK791_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOH91810.1"
                     /translation="MTKMTECEIDSVRVSLTNQDRVVVLKDKNSERYLPIWIGLFESE
                     ALTIALQGVQTARPLTHDLLLSTIQSLGAQFVRVEIVAIDEDVYFAQLVLLLEGKQIN
                     IDCRPSDALVMMVRARVPIFIAEDILEKVAIQPEDRVPYDLSVDPVEGDDGELEDLSV
                     FSDFLSTLEETPDAIIEDAEIEIDSDEDDDDDDDDEDIDLDDILFGPTINNN"
     gene            1280..2647
                     /gene="dnaB"
                     /locus_tag="PK791_00430"
     CDS             1280..2647
                     /gene="dnaB"
                     /locus_tag="PK791_00430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOH91811.1"
                     /translation="MSDQDYQTQNENLVPTQLYDRELEEALIGAILINPDAFVDVSPK
                     LDSKDFYIHKHEWIWDAFKSLLHADRNIDLLTVKQELESAGRFDDSGGMAYLVGLTNL
                     VPSSLHAEAYAETIKGLALRRRLLKSANEIAKIAHRKDMTIDAVIEESENAIFGVSQA
                     RFSRDLVPLSSVAMDYQRRIQEVSKKTEGLSGLETGLRSLDLVLDGLQKEDLIIVAGR
                     PGMGKTGFLLGIAKHVGMRLKRNVAIFSLEMSNTQLLQRMLAQETGIDSQKLRSAKLT
                     DEEWTRTFHALSLLGESRIFIDDTPAITPLQMRSKCQRLKMEEGLDLVIVDYLQLMSS
                     DTRTENRVQEVSYISRYLKVLARDLGVPVLAAAQLSRAVEQRQDKQPVLSDLRESGSL
                     EQDADIVMFINRPDQMNEDSPFHNLARLAVAKHRNGPTHSGIELVFIERLAMFRDATR
                     QEMPR"
     gene            2644..3324
                     /locus_tag="PK791_00435"
     CDS             2644..3324
                     /locus_tag="PK791_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOH91812.1"
                     /translation="MSQGLRSPGGFNTNTHLVALPRDFVSACVGLSGEAVLLLVHYFS
                     ICSSAKDSVAYVEQEKLLENFSKWHGSQKQKAKTALDELLEKALLILWRENETDYLIA
                     GTPEGRFLYQNLSLGEKSLLEQTSSNLSLEDERPNIFKLYETHIGIITPMMAEQLKQD
                     EIDFPAEWIEDAIREAVERNARNWKYVKAILLAWKERGRDKRDEGHQSDIEAFRRLTR
                     QQQERDQP"
     gene            3251..4666
                     /locus_tag="PK791_00440"
     CDS             3251..4666
                     /locus_tag="PK791_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH91813.1"
                     /translation="MKDIKAISKHLEDSLGNSKSETNPEQEISYRQSPADQHFGDPDC
                     PYCLGLGFVGHEVPIDHPDYGKLYICSCRLKRVQSDQQNALFEFSNLGSLRDLTFETF
                     KPRGRVGLASQQAASLQQAYNSARHFADNCQGWLLLTGTYGSGKTHLAAAIANETLNR
                     GVPTLFLTVPDLLDWLRFSFSSDSESSFESRFAEIRNVAVLILDDFGTQSSTAWAQEK
                     LFQIINHRYISQLPTVITTNYRFNDFEGRIRSRLTDPEIVTRVDILAPDYRNPTDEVG
                     HPELSTLNLHNKQQFDTFSLRANEPGMLAEDKASLKHALDIAQAYAKNPQGWLLFQGP
                     YACGKTHLAAAIGNYQAALGAPPLFVGVPDLLDYLRAAFAPNSTQSLDKRFDEIRSTR
                     LLILDDLDTQSATPWAREKIYQLFNYRYVAELPTVMTTSRQPSELDQRLFSRLSDTRL
                     CQVVYMSAPAYTGKSTRKTHK"
     gene            4768..6936
                     /gene="glgP"
                     /locus_tag="PK791_00445"
     CDS             4768..6936
                     /gene="glgP"
                     /locus_tag="PK791_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOH91814.1"
                     /translation="MEKINRHLPDGFNLPYRIKRLAELAHNLWWTWNPEATRLFKEID
                     ELNWNLSTHNPVVFLRKVDHQVFEDLIKDRYFLERYDSVMRAFDAYMQEKDTWFNVNY
                     PQLADEHIGYFSFEYGLHESLKVYAGGLGILSGDHVKEASDLDLPFTAVGFLYTYGYF
                     AQRITEDGWQVARNVEINFNDLPIASLYDKNGEPVKISVDLPGRTLWARLYELKVGRV
                     TLVLLHTDLPENSPQDRQLTDKLYISDPELRISQEILLGIGGMRALDRLGIKPTIYHM
                     NEGHSAFLSLERLRQLLASGKTMEEAKEIVSASNIFTTHTPVPAGNDEFPIWLVDKYF
                     SNYWGELGLDREGFLDLAKVRNDWADEVFSMPVLALRLSNYRNGVSKLHGKVSRGMWQ
                     GLWQDKAEDDVPITSVTNGVHTLSWLARRITELYKRTFGPDWINHIDDPKMWEGIERI
                     DDAELWQIRFHLKRKLVNYMVTRARYQWANNNVHPVQTVASGVLLEPSALTIGFARRF
                     ATYKRANLIFHDWDRLLRIITNPKKPVQIIFAGKAHPNDEPGKRMIQEVYRAVKDARN
                     GGRLVFLEDYDMDMARYLTQGVDVWLNNPRRPREASGTSGMKAAMNGVLNFSVLDGWW
                     AEGYNGVNGWAIGDETEYTDLDAQDKADANSLYDTLENEIVPLYYTKRSADGLPADWL
                     QRVKDCMRSISPAFSMTRMVKEYMTELYHPAMLGAQKYKE"
     gene            6945..7496
                     /locus_tag="PK791_00450"
     CDS             6945..7496
                     /locus_tag="PK791_00450"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOH91815.1"
                     /translation="MGPFVLEFSKETILISLLIFAVRVVGMALDTLRIMLVMRKKQGI
                     AWVLGFLETVIYVLSIGVVLDDVSNVLKVFAYSAGFATGSVVGMWFESKLAIGHAHII
                     IITKLPGSIVADALRAGDFAVTEIHATGKDGNVWLCDLTVARKKVKDVETIVREADPD
                     AFVTIEDITPLHRGYWGSGQLSR"
     gene            7664..10180
                     /locus_tag="PK791_00455"
     CDS             7664..10180
                     /locus_tag="PK791_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91816.1"
                     /translation="MEIDSSKFYFGQAYDLTEGKVIPDKLTFYDPEDLVTHGVIVGMT
                     GSGKTGLGIILLEEAALQGIPAILIDPKGDLTNHLLHFPDLQADDFAPWIDEDAAKRD
                     GQTVQEAATAAASNWAKGLERSGIDKARMEKLSTNVDYVVYSPGSTSAFPISILSSLR
                     APKLDWEENKEMLRENISSTVTALLGLVGFKNIDPIRSREHILLSNIFEHNWSQGADL
                     NLESLILQVQNPPFDKLGVFAMTKFYPEKDRFELAMLLNNFIAAPSFENWLKGQALDI
                     GSILYAPDGKPRHSVFYLAHLSDQERMFFVTLLYTAIETWMRSQAGTTNLRALVYFDE
                     IAGYLPPVANPPSKPIILRMLKMARAFGVGLVLSTQNPIDLDYKALSNAGTWMIGRLQ
                     TEQDKARLLDGLDSIAGGYDRAYFDKAITALGKRVFLLHNVHAKAPEVFTTRWAMNYL
                     PGPITREKLEDLNRLVGADLAILHASMQAEEEGSASPVSFAEPGTQATLNTEPKLPSS
                     VDLAYLPINVNPGAAYDQAMRQNVAHGEQPSFHYEALLMGQATVYYANRTHNIDTQQK
                     IAVRLRDLQGRGLVRWEDYTTEAIDLHAVDSTPLSNATFGSLSYPVDDEKTINELKKD
                     FVEWIYRNAPVTVYRNETLKLSSRVDESLEDFKARCEEASQGDKEALRKKITDDYAKK
                     KSAAENRLMKEEMKLEKLEETLKGRRLEEGATGLTTALSWLGSFNKTVGKVIGGRRKS
                     ITSSLTKRRLTASAKKAVEESELMVERYKEELEAVDEQMQAELDALQDKHSEMQGEIR
                     EIAINPLKKDIVVEFFGLAWEPQYAYKAGDRIVMVPANEV"
     gene            complement(10276..11292)
                     /locus_tag="PK791_00460"
     CDS             complement(10276..11292)
                     /locus_tag="PK791_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger protein"
                     /protein_id="HOH91817.1"
                     /translation="MARKILGYVELIWTCDSCGTKNPGAIKTCTACGAPQAVNVRFEQ
                     VDANTFNFIKDEALIRMAQSGPDKHCPYCGTRNLADSLVCRQCSADLTVGAKSRPSGM
                     AVGEDAQPTEYEIDDSTLPPEQAHATKPLSRKVLLILMLVILIICGYFAWNTIRANRT
                     KDIKATVVSTAWERTMAIEAYTTVTESDWLSDIPSGASAYNCSSQLRDTSDTYVPGSI
                     ENCSEPYTVDTGTGIGEVVQDCVYEIYEDYCDYETMAWVMADTVTTQGTDVYATWPDP
                     NLTQSQRISGRSESYTIYFDANGQSYILRTSDYEVFQQATPGSTWKLAVNKQGDVRKA
                     EPQN"
     gene            complement(11306..>11630)
                     /locus_tag="PK791_00465"
     CDS             complement(11306..>11630)
                     /locus_tag="PK791_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91818.1"
                     /translation="SSDTLKSSVPLGIAGAFFTVLVFWSIWQFLTLSCFFLQDEPSMK
                     LAWKNGWAIFVRLRSFSLTIGIIIFIFSLFSVFTFIPLVLAAEGLIAITGVRAVQATI
                     LPEQG"
BASE COUNT         3044 a         2701 c         2916 g         2969 t
ORIGIN      
        1 ggatgtgttt atgtttgacc tcgttaaaca gtcgcaggcg gttgatgagt ttttgcacgg
       61 taaaaggcag gcgcaaaaga acgttcgcct gaggtgcgtt ggtgatggct tcgtcttcgg
      121 cgatgatgag aatgattggc aaatcaggcc aggcagtacg aatccagttg gtgatgcgca
      181 ggccatttgt gcgcaaactc atggcgttga taacgaccac attgggcatc aggtctggga
      241 ttgtttctaa tgccgtggcg ccactggtga cggtggcgac atcaaatcct ttatcagaaa
      301 gctcttgcgc aaaattattc ttaagacggc gtttgccttc tattaataat accgataatc
      361 cattttccat taatacaaaa ccctaataat tccaaaacaa agcgcgcaca aaatgggcct
      421 aaaagacctt aaaaatgtta aaacgtgctg atttggctat tatagtcgaa agtttagaaa
      481 tttgcttaga ttcaagagtg caattttcat tttttccatc tatttcagta tgtaaaaata
      541 ttatcgcaaa gccaagctta aaatatgtga ttaaagcgtc atggcgtgtt agaatcaaaa
      601 gtgtatttaa ttccgggatt gatgtactat gacaaaaatg acagaatgtg agattgatag
      661 cgtcagggtt agtttaacca accaggatcg cgttgtggtg cttaaagata aaaacagcga
      721 acgttatttg cccatctgga ttggtctgtt cgagtccgaa gccctgacca tcgctttgca
      781 gggtgtgcaa accgcgcgac cgctgacgca tgacttattg cttagcacta ttcaatccct
      841 tggcgcgcaa tttgtgcgcg ttgaaatcgt tgccattgat gaagatgttt attttgcgca
      901 gctggtcttg cttttggaag gcaagcaaat caatattgat tgccgtccgt cggatgctct
      961 tgttatgatg gtgcgcgcgc gtgtacccat ttttatcgcg gaagatatct tggaaaaagt
     1021 tgctatccaa cccgaagacc gtgttcctta tgacctgtcg gttgacccag ttgaagggga
     1081 tgacggtgaa ctggaagacc ttagcgtctt tagcgacttt ctttccacac ttgaagaaac
     1141 gcctgatgca attattgaag atgccgaaat tgaaattgat agcgatgaag acgatgatga
     1201 cgatgacgat gacgaagata tcgaccttga cgatatcctt tttggcccta caataaacaa
     1261 taactaataa agaattgcca tgagtgatca agactaccaa acccaaaacg aaaatcttgt
     1321 cccaacccaa ctttatgacc gtgagttgga agaagctctg attggtgcta ttctcatcaa
     1381 tcctgatgct tttgttgacg tctcgccgaa actcgactca aaagattttt atattcataa
     1441 gcacgagtgg atctgggacg cgttcaagag tttgctccat gctgaccgaa atattgacct
     1501 gcttaccgtc aaacaagaac tcgaaagtgc gggccggttt gatgacagcg gtggtatggc
     1561 ttatttggtt ggactgacga atcttgtgcc cagttctttg catgctgaag cctatgcaga
     1621 aacgataaaa ggacttgctt tgcgccggcg tcttttgaaa tcagccaacg aaattgctaa
     1681 aatcgcccat cgcaaagata tgaccatcga cgcggttatt gaggaatctg aaaacgcgat
     1741 ttttggtgtt agccaggcgc gtttcagtcg cgatttggtg cccctttcca gcgtggcaat
     1801 ggattaccag cgacgtattc aggaagtcag caagaagacc gaaggcctta gcgggcttga
     1861 gacgggctta cgttccctgg atttggtttt ggatggtttg cagaaggaag acttgattat
     1921 tgtcgcgggg cgcccaggca tgggtaaaac cggcttttta ctgggcattg ccaaacatgt
     1981 gggcatgcgc cttaaacgca atgtggctat tttctcgctg gaaatgtcca acacgcagtt
     2041 gcttcagcgt atgcttgccc aggaaacagg cattgactcg caaaaattgc gatccgccaa
     2101 acttactgac gaagaatgga cacgcacttt tcacgctttg agccttttgg gtgagtcgcg
     2161 cattttcatc gacgacacgc cagcgattac accactacag atgcgctcta aatgtcagcg
     2221 tctaaagatg gaggaagggc ttgacttggt cattgtggac tatttgcagc tgatgtccag
     2281 cgatacgcgt accgagaaca gggtgcagga agtttcgtac atttcgcgct atctcaaagt
     2341 gcttgcccgt gaccttggtg tgcccgtttt ggctgccgcg cagctttcgc gcgcggttga
     2401 acaacgtcag gacaagcaac ccgtactttc agacctgcgc gaatcgggct cacttgaaca
     2461 ggacgcggat atcgttatgt tcatcaatcg acctgaccaa atgaacgaag acagtccttt
     2521 ccacaacctg gcgcggctcg ctgtcgccaa gcatcgtaat ggtcccactc acagtggtat
     2581 tgagttggtt ttcatcgagc gtttggcgat gttccgtgat gctacccgtc aagagatgcc
     2641 cagatgagtc agggattaag gtcgcctggt ggctttaaca caaatactca tcttgtcgcg
     2701 cttccgcgcg attttgtgag tgcctgtgtg ggcttgagtg gcgaagccgt tttgcttttg
     2761 gtgcattact ttagtatatg ttcatcagcc aaggactctg tggcctatgt tgaacaggaa
     2821 aagctgctgg aaaacttctc gaaatggcat ggcagtcaga aacaaaaggc taaaacggcg
     2881 cttgacgagc ttttggaaaa ggcattattg attctctggc gcgagaatga gaccgactat
     2941 ctcatcgcgg gaacgccaga aggacgattt ttatatcaaa acttaagttt gggcgaaaag
     3001 agtcttttgg aacaaacatc ttcaaaccta agtttagaag atgaacgccc gaacattttt
     3061 aaactttatg agacgcatat cggcattatt acacccatga tggccgaaca gctaaagcaa
     3121 gatgaaattg attttccggc agagtggatt gaagatgcca tccgcgaggc agtggaacgt
     3181 aacgcccgca attggaaata tgtaaaagcc atcttgcttg cctggaaaga acgaggacga
     3241 gacaaacgag atgaaggaca tcaaagcgat atcgaagcat ttagaagact cactcggcaa
     3301 cagcaagagc gagaccaacc ctgaacaaga aataagctac cgccaaagtc ccgctgacca
     3361 gcattttggt gacccagact gcccctattg tttgggtttg ggctttgtgg ggcatgaggt
     3421 gcccattgat catcctgatt atggtaagtt gtatatttgc agttgccggc taaagcgggt
     3481 gcaaagtgac cagcaaaacg cgctttttga gttcagcaat cttggcagtc tgcgcgacct
     3541 gacttttgag acatttaagc caagaggacg ggtaggtttg gcgtctcagc aagcggcatc
     3601 gctccagcag gcctacaatt ccgcgcgaca ttttgccgat aattgccagg gctggctctt
     3661 gctgacgggc acctatggct ctggcaaaac gcatttggca gccgccatcg ctaatgaaac
     3721 cttaaacaga ggcgtgccaa ccctgttttt gaccgtgcca gacttgctcg actggttgcg
     3781 tttttccttc agttccgaca gcgaaagcag ctttgaaagt cgttttgctg aaattcgcaa
     3841 tgtggcggta cttattttgg atgactttgg cacgcaaagt tcgacggcat gggcgcagga
     3901 aaaactcttc caaattatca atcaccgcta tattagtcag cttcctacgg tgattaccac
     3961 aaattatcgc tttaatgatt ttgaaggacg catccgctcg cgcttgactg accctgaaat
     4021 tgtgacccgt gtggatatcc ttgcgccgga ctatcgcaat ccaaccgatg aagttggcca
     4081 tccggagctc agcacgctga atctgcacaa taaacaacag tttgacactt tcagcttgcg
     4141 cgccaacgaa cctggtatgt tggctgaaga caaggcttca ctcaaacatg ctttggacat
     4201 tgcccaggcc tatgccaaaa acccgcaagg ctggctgctt ttccaggggc cttatgcctg
     4261 tggcaaaacg catctcgccg cggcaatcgg gaattatcag gccgcgcttg gcgcgccacc
     4321 gctttttgtg ggtgtgcccg atttgctgga ttatctgcgc gcggcttttg ccccaaacag
     4381 cacacaaagt ctggacaagc gctttgatga aatccgctca acgcggttgc ttatccttga
     4441 tgacctggac acgcagtctg ccacgccatg ggcacgcgag aagatttatc agcttttcaa
     4501 ctatcgctat gtggcagagt tgcccacggt gatgaccaca tcgcgccagc cttctgaact
     4561 tgaccaaagg ctttttagcc ggctgtctga tacgcgcttg tgtcaggtag tttatatgag
     4621 tgcaccagct tatacaggaa agagtacaag aaaaacacat aaatgaaaat aacggtataa
     4681 tggtgaagtt aaagtagttg atacatctac tatattgaga atatcatggc gctttaggtg
     4741 ccatatcagt aaaaaggaaa aggaaatatg gaaaaaataa atcgacattt gcccgatggc
     4801 ttcaatctgc cttatcgtat taaacggctt gccgaacttg cccacaatct gtggtggaca
     4861 tggaacccgg aagcgacccg tctttttaag gaaattgatg aacttaattg gaatctctcg
     4921 acccataatc cggttgtttt tctacgcaaa gtagatcacc aggtttttga agacctgatt
     4981 aaagaccgct attttctgga gcgttatgat agcgtgatgc gtgcgtttga cgcttatatg
     5041 caagagaagg atacctggtt taacgtgaat tatccccaac tggctgatga acacattggc
     5101 tatttctcat ttgaatacgg cttgcatgaa tcgctaaaag tttatgccgg tggcttgggt
     5161 attttaagtg gtgaccacgt aaaagaagcc agcgacctgg atttgccctt taccgcggtg
     5221 ggtttcttat acacctacgg ttattttgca cagcgcatta ccgaagatgg ctggcaggtc
     5281 gcccgcaatg ttgaaatcaa cttcaacgat ttgccgattg catcacttta tgacaagaat
     5341 ggcgaacccg tcaagatatc tgttgatttg ccaggacgta cactttgggc acggctttat
     5401 gagctcaaag ttggtcgggt tacccttgtg cttttgcata ccgacttgcc tgaaaacagc
     5461 ccgcaagacc gccagctgac cgataaactc tatatcagcg acccggaact gcgtatctcg
     5521 caggaaatcc tgttgggcat cggtggtatg cgcgcgcttg accgcctggg cattaaaccc
     5581 acaatttatc acatgaacga aggtcattcc gcgttcctga gtctcgaacg tctgcgccag
     5641 ctcctggcca gtggcaagac gatggaagaa gccaaagaaa ttgtcagtgc ttcaaacatc
     5701 tttaccacgc acacacctgt gcctgccggt aacgatgaat tccccatttg gctggttgat
     5761 aaatacttta gcaattactg gggcgaactc ggactggacc gcgaaggctt tttggacctg
     5821 gctaaagtca ggaatgactg ggctgatgaa gtctttagta tgcctgtttt ggccttgcgg
     5881 ctttcaaatt atcgcaacgg cgtttccaaa ctgcatggca aagtttcgcg gggtatgtgg
     5941 caaggacttt ggcaagacaa agctgaagat gatgtgccta tcacctctgt taccaacggt
     6001 gtgcatacgc tcagctggct tgcccgtcgc attactgaac tttataagcg cacctttggt
     6061 cctgactgga ttaatcacat tgatgatccc aagatgtggg aaggcatcga aagaatagat
     6121 gacgcagaac tctggcaaat ccgcttccat ctcaagcgga aactggttaa ctatatggtt
     6181 acccgcgcgc ggtatcagtg ggcgaataac aacgttcacc ccgtgcaaac tgtggcaagt
     6241 ggtgttctgc tggaaccgag cgcgcttact attggtttcg cgcgtcgctt tgcgacctat
     6301 aaacgcgcta atttaatctt ccacgactgg gaccgcctgt tgcgtatcat cacgaatccg
     6361 aagaaaccag tgcaaatcat ctttgctggt aaagcacatc ccaacgatga acccggtaaa
     6421 cgcatgattc aggaagtcta tcgcgcggtg aaagatgccc gcaacggtgg cagactggtc
     6481 ttccttgagg attacgacat ggatatggcg cggtatctga cccagggcgt ggacgtgtgg
     6541 ctcaataacc cacgcagacc ccgcgaagcc agtggcacga gtgggatgaa agccgctatg
     6601 aacggcgtgc tcaacttctc ggttctcgat ggctggtggg cggaaggcta taacggcgtt
     6661 aatggctggg cgattggtga tgaaaccgaa tacaccgatt tagacgctca agataaggca
     6721 gatgcaaaca gtttgtatga cacgctggag aacgaaatcg tgccactgta ctatacaaaa
     6781 cgcagtgccg atggtttgcc tgctgactgg cttcagcgcg taaaagattg tatgcgcagc
     6841 atctcgcccg cattttcgat gacccgcatg gtcaaagaat atatgaccga gctttatcat
     6901 cccgcaatgc tcggtgctca aaaatataaa gaataggaga ataaatgggt ccttttgtac
     6961 ttgaattttc taaagagacc atattaatct cattgctcat tttcgctgtg cgtgtggttg
     7021 gtatggcgct tgatacctta cgtatcatgc ttgttatgcg taagaaacaa ggcattgcct
     7081 gggttttagg ctttttggag acggttattt acgttctctc cattggtgtt gtcttggacg
     7141 atgtcagtaa tgtgcttaaa gtgtttgctt attcagcagg ttttgccaca ggtagtgtgg
     7201 taggcatgtg gttcgaatca aaattagcca tcggacatgc ccatatcatt atcattacca
     7261 aattaccagg ttcaatcgtt gctgatgctt tgcgcgctgg tgactttgct gtgacggaaa
     7321 ttcacgctac gggaaaagac ggcaatgtgt ggctgtgtga ccttaccgtc gcacgtaaga
     7381 aggtgaaaga cgttgaaact atcgtccgcg aagctgaccc tgatgccttt gttaccattg
     7441 aagacatcac cccactccat cgtgggtatt ggggctctgg gcaactgagt cgatagtctt
     7501 aaattgtttg aatgagaaga cccctggcta aaaccggggg tctttttttg actgtgttcg
     7561 gcaaacagaa acaaaaccgg gaaatgatga ggggtaatgg atttgcttaa tttttactat
     7621 caatcgctta taattaggca tcgctagtaa caaggagatg aaaatggaga ttgattcatc
     7681 aaaattttat ttcggccaag cctacgacct gacggaaggc aaagttatcc cagataaatt
     7741 aacattttat gaccctgaag acctggtaac ccatggcgtt attgtgggta tgaccggctc
     7801 gggcaaaaca ggtttgggca ttatcctgct tgaagaagcc gctttgcagg gcatcccggc
     7861 tattctcatt gaccccaaag gcgacttgac caaccatctt ttacacttcc cagatttgca
     7921 ggcagatgat ttcgcgccct ggatagacga agacgctgcc aaacgcgatg gacagaccgt
     7981 gcaagaagcc gctaccgcgg ctgccagcaa ctgggcaaaa ggacttgaac gctccggcat
     8041 cgacaaagcc cgcatggaga aactttccac gaacgttgat tacgttgttt actcgcctgg
     8101 ttcgaccagc gcattcccga taagcattct cagctcactg cgtgcgccaa aacttgactg
     8161 ggaagaaaac aaagaaatgc tgcgcgagaa catcagcagc acggttaccg ccttgcttgg
     8221 tttggtgggc tttaaaaaca ttgaccccat ccgctcacgc gaacatattt tgctgtcaaa
     8281 tatctttgaa cacaactgga gtcagggtgc tgacctaaac cttgaatcac tcattttgca
     8341 agttcaaaat ccaccctttg acaagctggg tgtcttcgcg atgactaagt tttatcctga
     8401 aaaagaccgc tttgaactgg ctatgctgct caacaacttt atcgccgcgc caagtttcga
     8461 aaactggctc aaagggcagg ctttggatat cggatccatt ctctatgctc ctgatggcaa
     8521 accccggcac agcgtctttt atctggcgca tttgagtgac caggaacgca tgttctttgt
     8581 aaccttgctc tatacagcga tagaaacctg gatgcgcagc caggcgggca cgacgaattt
     8641 gcgcgcctta gtctattttg atgaaattgc tggctattta ccgcctgttg ctaatccgcc
     8701 atcaaaaccc atcattctac gcatgctcaa gatggcacgc gcttttggtg ttggcctggt
     8761 gctttcgacc caaaacccga ttgaccttga ctataaagcg ctcagcaacg ccggcacctg
     8821 gatgatcggg cgcttgcaga ctgaacagga caaagcgcgc ctgctggacg gtttggacag
     8881 catcgctggt ggttatgacc gcgcgtactt cgacaaagct atcaccgcgc tgggaaagcg
     8941 ggtcttttta ctgcataatg tgcatgccaa ggcgcccgaa gtctttacaa cccgctgggc
     9001 gatgaactat ttgccaggtc ctatcacacg cgagaagctg gaagatctca atcgcctggt
     9061 tggcgcggat ttggcaatct tgcatgccag tatgcaagct gaagaagaag gaagtgcttc
     9121 gccagtcagt tttgctgaac ctggtacaca ggcgaccttg aacacagaac ccaaattgcc
     9181 ctcgagtgtt gacctggcct atctgcccat caatgttaac cccggcgcgg cttatgatca
     9241 ggccatgcgt cagaatgtcg cccatggaga acagccaagt ttccactatg aagcgctttt
     9301 gatgggacaa gccaccgttt attacgctaa tcgcacgcat aatatagaca cacaacaaaa
     9361 aatcgctgtg cgtttgcgcg atttgcaagg acgcggtttg gtgcgctggg aagactacac
     9421 cacggaagcc atagatttgc atgctgttga cagtactccg ctaagcaacg ccacttttgg
     9481 ttcgctcagt tatccggttg atgatgaaaa gaccatcaac gaactgaaaa aagattttgt
     9541 ggaatggatt taccgcaatg cccccgtgac cgtctaccgc aatgaaacgc taaaactcag
     9601 ctcgcgcgtt gatgaaagtc tggaagactt caaggcaagg tgtgaagaag cctcgcaagg
     9661 ggataaagaa gccctgcgca agaaaatcac cgatgactat gccaagaaaa agagtgccgc
     9721 agaaaaccgg ctgatgaaag aagagatgaa actcgaaaag cttgaagaaa cgctcaaagg
     9781 tcgccggctt gaagaaggcg cgactggctt aaccaccgcc ttgagctggc tgggcagttt
     9841 taacaagacc gttggcaagg tgatcggtgg tcgtcgcaag agtattacca gttcactgac
     9901 caaacgccgt cttactgcca gcgctaaaaa agcggttgaa gaatcagaat tgatggttga
     9961 acgctacaaa gaagaacttg aagcggtgga cgaacaaatg caggcagaac tggatgcctt
    10021 gcaagacaaa catagcgaaa tgcaaggtga aatccgcgag attgccatta atccactcaa
    10081 gaaagacatt gtagtcgagt tttttggttt ggcgtgggag ccccagtacg cttataaagc
    10141 cggggaccgc atcgtgatgg ttccagctaa cgaggtttaa acgcgctttg actgactttt
    10201 aaaggctaaa tcaaacagct ttcatggcaa tcaccatgaa agctgttttt taatactaaa
    10261 agtaaattcg ggaaatcagt tttgcggttc cgctttacgg acatcccctt gtttgttcac
    10321 cgctaatttc caggtactgc caggcgtagc ttgctgaaag acttcataat cgctggtgcg
    10381 caggatgtaa ctttgaccgt tggcatcaaa ataaatggtg tagctttcgc tccgtccgct
    10441 gatgcgctgg ctttgcgtca aatttgggtc gggccaggtg gcatacacgt cagtaccttg
    10501 tgtggtgacg gtgtctgcca tcacccatgc catcgtctcg tagtcgcaat aatcttcgta
    10561 aatttcgtag acgcagtcct gcaccacttc cccgatacct gtgccggtat ctacggtgta
    10621 gggctcgctg caattttcta ttgacccggg aacgtaagtg tctgaagtat cacgcaactg
    10681 tgatgaacag ttataagcac tggcgccact gggaatgtcc gaaagccagt cgctttcggt
    10741 tacggtggta taggcttcaa tcgccatggt gcgctcccag gcagttgata ccacggtggc
    10801 tttgatgtct tttgtgcggt tggcgcggat cgtgttccag gcaaaatagc cacaaataat
    10861 cagaataacc agcatgagaa tcaaaagcac ttttcgcgat aagggcttgg tggcgtgcgc
    10921 ctgctctggc ggcagtgtgg aatcgtcaat ttcatattct gtgggttggg cgtcttcgcc
    10981 taccgccatg ccactcgggc gcgatttcgc gccgacggtc aaatcagcgc tgcattgccg
    11041 gcagaccagg gagtcggcaa ggttgcgtgt gccacaatag ggacaatgtt tgtccgggcc
    11101 ggattgcgcc atgcggatga gggcttcatc tttgatgaaa ttgaaagtgt tcgcgtcaac
    11161 ttgctcaaaa cgaacgttta cggcttgtgg cgcaccacat gccgtgcatg tttttattgc
    11221 gcctgggttt ttggtaccac agctgtcgca ggtccagatg agttctacat atcctaaaat
    11281 ttttcttgcc atgctagaga ttcctttatc cttgttcagg gagaatggtg gcttgaacag
    11341 cgcgcacacc agttatagcg ataagccctt cggccgcgag aaccaaaggg ataaacgtaa
    11401 agaccgaaaa cagtgaaaaa atgaaaatga taataccgat ggttagacta aaagaacgca
    11461 aacggacaaa tattgcccag ccatttttcc aggcgagttt catagacggc tcatcctgta
    11521 aaaagaaaca ggaaagcgtt aaaaattgcc aaatgctcca aaacaccaaa acggtgaaaa
    11581 atgctcccgc gatacccaaa ggcacactgg attttaaggt gtcggaactc
//
LOCUS       DAOSFC010000009        11551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_4695_length_11551_cov_5.592032, whole genome shotgun sequence.
ACCESSION   DAOSFC010000009 DAOSFC010000000
VERSION     DAOSFC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4695_length_11551_cov_5.592032"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="xseA"
                     /locus_tag="PK791_00470"
     CDS             complement(<1..947)
                     /gene="xseA"
                     /locus_tag="PK791_00470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOH91819.1"
                     /translation="MNYTTIIMFDFEDLQPTIMTVKALNSYLRELLETDDILRDIWVK
                     GEISNFTRASSGHLYFTLKDTDAQVKCVMWKGSTFGLRFDPREGMAVEVHGSMSIYSA
                     TGQVQLYVSEMRQAGQGQLYAEFLKLKAKLQAAGLFDKKKPLPRFPHHIGIVSSATGA
                     ALQDMLHALDRRLPTARVTIAPSTVQGADAPASIIRALNALNRIEDLDVILLGRGGGS
                     IEDLWCFNDEALAYVIFNSRVPVISGVGHETDFTIADFVADLRAPTPTAAAELASPVT
                     VDDLLQNLTDRSARLEQSLSAIFTMHQQKFQLAQSALERV"
     gene            996..1664
                     /locus_tag="PK791_00475"
     CDS             996..1664
                     /locus_tag="PK791_00475"
                     /inference="COORDINATES: protein motif:HMM:NF026037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91820.1"
                     /translation="MKNITKLVLLTLTLSLLLAGCNLIANQTQTSDEFIQTIADYAVK
                     LTVQASEIDLLKQALTESPDSCPTCVVCPTTTPCPVNTPCPVCPTVAPEPTPTATKGI
                     PVGTASISGKLIYPSDHIPPQRVIAFEVKTGYYYWVRTATGNASYTISNLPAGTYKVV
                     SYKISESPTGLKAGYTPFAACNFTCAEDHSLIEFELKEGEHKTGIDPIDWYAPEDVDW
                     PDEP"
     gene            1687..3525
                     /locus_tag="PK791_00480"
     CDS             1687..3525
                     /locus_tag="PK791_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOH91821.1"
                     /translation="MSSKKTLFLQITLILLLLLSTLTACENGQSKAENFIVSVQMPQR
                     GLSQGQNFVLTLTLQNNSIKNKEVESIVLPAAFIKGVNAFNTQPAMPRGINSNGDWVF
                     EYERTIAPQGLEQVAFYFETAPTGHFEATGVVNTSERSYEFALDLTVLGVNPADWSPG
                     AAKPISDDAVYEPPYLSVVKIDAFAEQDGQRTQVWSGNGAIISSDGLILTSADVVLSD
                     RYFQLTDLLVSLTVDAEAPPVPSYRASIVQADAKLGLAIIKPRETLDGKAIDYSLLDL
                     PVLALGDSRVLEQDAELRVTGYPQAPNTSELMSERNLRLNDFGAELPFGEGSLWHADR
                     AIETGYLGAPVLNEAGELVAVPIHKSPITFDPRTVTCEPVIDGNRDGRIDALDPCPRT
                     SLPIDTLRPIHLAQEMIDAARRGEISISMAGIAQPLSHTPVKLLDTDDFSNNRNQWKI
                     TGNDFGEQKIEDGAYLFKLHKPMTQLWSSVGYGYDQPMQIDVEARVTAPVYNSEFGVI
                     CGLTETERTVFAVSENGYFAIWKVQNDAMHYLQDWRYGGFDAEQTLNLSVQCGQGWYQ
                     FAVNERVLAQGTDPDFKPGLTGLYVASYDKGGLTVHFDNFSVSIIE"
     gene            complement(3573..5372)
                     /locus_tag="PK791_00485"
     CDS             complement(3573..5372)
                     /locus_tag="PK791_00485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOH91822.1"
                     /translation="MTPTRWDLSNIYTGLDDPQLVEDINYIKTIAAELTAIYETKLEP
                     ALSSDISDEALSQLLNQIVDTRNTVSLVGNKIGAFLGATLSVDSFNKTAEQLYSKVQS
                     EMLPLGNLSNRISAWLGKLGDRLPKALQYPGSAHEHSFALLEEAEQSRYMMSEAEEEL
                     ANELSLSASSAWDNLQGVLTSQKSVEFELDGKMQTLPLPALINLRSHPDADTRERAYK
                     LEMQVFEEMKEPLAACMNGIKGEVNVLNRRRGRQDALHSALDMARIDRETLDAMMGAM
                     QDSLPMFRDYFRAKAKKLGHDKLPWWSLFAPVGSANKNYSFSEAKELILENFAKFSPE
                     LAELAQTAFESNWIDAEQRAGKRGGAFCMGIPVVKESRIMSNFDGSFDQVMTLAHELG
                     HAFHNYCIYQAGKTPFQSRTPMTLAETASIMCETIVLTALLKNPSSPEEELMLLETAI
                     ASDAQTIVDIMSRYLFEMEVFIRREKGTIPADDISEIMLQAQRDTYGDGIDPEVMNKY
                     AWTWKPHYYSAHLSFYNFPYAFGTLFGKGLYAIYLEKGDAFLEDYKALLSSTGEANAA
                     DLAARFGINIRDKAFWKSSLDTLKPMIERYIAL"
     gene            complement(5536..6225)
                     /locus_tag="PK791_00490"
     CDS             complement(5536..6225)
                     /locus_tag="PK791_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOH91823.1"
                     /translation="MPFSPTYFIFILPGLVLSIIASIAVNSAYKKWSQVRNTRNLTGL
                     QVADYLKSRYNLDEISFTQTQGTLSDHYDPRSKTLALSQNVGAVPSVAAMAITAHELG
                     HAAQDRDNYGPMKLRRVLVPVVNIGSNVGMILVIIGLVLNLLGLATIGIILFASTTVF
                     SLVTLPIELDASKRAKVMLEESQLMTSDEERKGVNKVLNAAAWTYVAGLVTSVLQLLY
                     YVSLISGRRRR"
     gene            complement(6252..7601)
                     /locus_tag="PK791_00495"
     CDS             complement(6252..7601)
                     /locus_tag="PK791_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH91824.1"
                     /translation="MQNKSNRTILTVILIVLIVVTSYGAGVATASLVKFNPIRIALGY
                     DKLGNTVGVSDPCPACLCPDGDGVTEGLECDPCPAIQFGADAGKCSEYVSSSTPRELQ
                     TLFKPFWESWNYLHRYYVDQPLDDVELMRGAIQGMLAATGDKHTSYMDPHTYEQANAE
                     MEGSYTGIGAWVNTSGEYVEIVSPMKGSPAEEAGLKPKDIIIAVNGEDVTGTAGDIVL
                     QSILGPAGEVVVLTIRRGDEIFDVSITRRVIEIPVVDYEMLENDIAYIALYTFNDKAV
                     EQVSNALNELMAQNPKGLIFDLRDNGGGYLYAAIDISAMFIEDGVILYEEYGDGTRDT
                     YRAEGDAIAPNIPMVVLINGGSASASEIVAGALQDHGRAKLIGEVSYGKGSVQSWIDL
                     SDNQGGVRITIARWLTPDGNQIAEIGLTPDIEVPYTEADFEAGIDPQRDAAIEYFNK"
     gene            7919..9094
                     /locus_tag="PK791_00500"
     CDS             7919..9094
                     /locus_tag="PK791_00500"
                     /inference="COORDINATES: protein motif:HMM:NF023524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HOH91825.1"
                     /translation="MKNMFNYIMTNYLTAKKEDFRNHPVANFIRNKAVNIISEKAYID
                     ETKYRITGSAGMGQWTHFPWIAIFDIDITTSAQRGFYPVYLFTEDMRGVYLSLNQGWT
                     YFKGEYGTRLGIKNISIVSKFWKEKLFSNLSDFEEPKISLITQSLNHENATGYERGHI
                     MGTYYDLKSLPDTKNLVNDLRNLLGVYREMKGIMGNNSFESMVDKVINKQDSLDNEDG
                     FYSIEPDRSHLEIRDKSVLITPSQVDFLELQKQQKELGLSGEELIIKYEQNKLISAGR
                     QDLAQKVLHESKEHGDGAGYDILSFNSKGDTIFIEVKTTTRDIHTTFFLSENERRFSD
                     ENASSYKLYRIYDYKEENDSFAFYIIEGSMSKTLDLEPTAYYVKNIHTKVQHVSSEK"
     gene            complement(9128..9769)
                     /locus_tag="PK791_00505"
     CDS             complement(9128..9769)
                     /locus_tag="PK791_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOH91826.1"
                     /translation="MMNQISILLIDLDDTVYPSSLNIWPLFMERIQSYIHEVLGVPLE
                     ETAELQHRLFVQYGTTLRGLQVERGVDMHDYLNWVHSVDLSDYMAPDPALRKALESLP
                     QPKWIYTNASVAHAENVLGLMNLRDLFVDIIDVVATAPYCKPEKESYQIALNLIGNPK
                     PENCLFLDDRSINLDTARQLGIHTLQVKPEPDGDHPYISHLIHLPEYLNKTRF"
     gene            complement(9766..10359)
                     /locus_tag="PK791_00510"
     CDS             complement(9766..10359)
                     /locus_tag="PK791_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOH91827.1"
                     /translation="MKTTFRKNITVFAGSADGLPETHMQIAWQLGEALAKQNRTLIYG
                     AGSTGLMGAVANGILENHGEVIGVVNDALNLEHLIHATLTKLETVRDIQTRQKRMLEL
                     ADGIIALPGGYGTYYETVEALTWAQIGLHTKPVGLLNIEGYFDPFVRMVEHSIALGYI
                     YPEHRGLFIVADTPIDLLQKLDEFTPPQNMNRWLDRT"
     gene            complement(10376..>11551)
                     /locus_tag="PK791_00515"
     CDS             complement(10376..>11551)
                     /locus_tag="PK791_00515"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH91828.1"
                     /translation="FIDKPIIKVLTGLRRSGKSVLLEQIQDRLIMQGIAPSQIFTLNF
                     ESAKAMHIRDDQDLIHELQNQPFDRNKKVYIFLDEIQEVQNWERAVNSLRVDFNSDIY
                     ITGSNSSLLSGELATYLAGRYVEFQIYPFSFLEFTQLLEHTPLKLDKDAAFNLYINLG
                     GMPFLHHLDFDVEPSKQYLKDLYASVVVKDIVSRHNIRDVDLLQRVILFLMSNIGNPF
                     SARSISAYLKNEYRAVAPETVINYIKATLDACLFYPAKRQDLRGKRILQIDEKYYLAD
                     HGIRQAVYGENFRDIHLILENIVFMELLRRGFDVTVGRIGEKEVDFVAEKADKKFYVQ
                     VSYIMNDEATFNREISPLLDIKDNYPKYIISMDPINRSTQGIKHMHILTFLLETESL"
BASE COUNT         3050 a         2533 c         2920 g         3048 t
ORIGIN      
        1 actctttcga gcgcgctttg ggcaagttga aatttttgtt ggtgcatcgt aaaaattgct
       61 gacagggatt gctcaaggcg ggcgcttcta tcagtaaggt tttggagcaa atcatcaacg
      121 gttactggac tggctagttc tgccgctgcg gttggcgtgg gcgcgcgcag gtcagctaca
      181 aagtcagcga tggtaaagtc ggtttcgtgg ccaacgccgg aaatgacagg tacgcgtgaa
      241 ttgaaaataa cgtatgccag ggcttcatcg ttaaaacacc ataaatcttc aattgagcca
      301 ccgccacggc caagtaaaat gacatccagg tcttcaatgc ggtttagcgc gttgagcgcg
      361 cgaatgattg acgcgggcgc gtccgcaccc tgaacagtgc tgggcgcgat ggtaacgcgg
      421 gcggtgggca gacgccggtc aagcgcgtga agcatgtctt gcagggcagc gcctgtcgca
      481 gagctgacga tgccgatatg gtgcgggaaa cgcgggaggg gctttttctt gtcaaaaagc
      541 ccagctgcct gaagcttggc tttcagcttt aaaaactccg cgtagagttg tccctgacca
      601 gcctggcgca tttcgctgac gtaaagctga acctgaccgg tagcggaata gatgctcatt
      661 gagccatgca cttcaaccgc catgccttcg cgcgggtcaa agcgcaagcc aaaggttgag
      721 cctttccaca tgacgcattt cacttgcgcg tcggtatctt tgagtgtgaa ataaagatgt
      781 ccggaactgg cgcgggtgaa attcgaaatt tcgcccttga cccaaatatc gcgtaaaata
      841 tcatctgttt caagtaattc gcgcagataa ctgttgagcg ctttcaccgt catgatggtg
      901 ggttgaaggt cttcgaagtc aaacatgatg atagtagtat aattcaaaaa gcgatgaaag
      961 ataacaataa gttctgaaag aaagtgtggt ggatgatgaa aaacattaca aaactggtcc
     1021 ttctaacttt gactttatcc ttgcttttgg ctgggtgtaa tttgatagca aatcaaacgc
     1081 agacatcaga tgaatttatc caaacgattg cggattatgc tgtaaaactt actgtccagg
     1141 ccagtgaaat tgacttgctc aaacaggcac tgaccgaaag cccagactct tgccccacgt
     1201 gtgtggtttg cccaacaaca acgccttgcc cggtcaacac accgtgtccg gtttgtccca
     1261 ctgtggcacc tgaacctaca ccgaccgcga ctaaagggat tcctgttggc acagcgagca
     1321 tttcaggtaa attgatttat ccatcggatc atatccctcc acaacgggtt attgcttttg
     1381 aagtcaaaac aggctattac tattgggtgc gtacagctac aggaaacgcc agttacacca
     1441 tttcgaactt gccagcaggt acttataagg ttgttagcta taaaataagc gagagtccaa
     1501 ctgggcttaa ggccggttat acacctttcg cggcatgtaa ttttacttgc gcggaggacc
     1561 atagcttaat cgagtttgaa ctaaaagaag gtgaacacaa aacgggcatt gatccgattg
     1621 actggtatgc gccagaagat gtagattggc ctgatgaacc ataaaactaa aatgatttaa
     1681 aaagaaatga gttctaaaaa aacgcttttt cttcaaatta cgctgattct tttgcttctg
     1741 ctatcgacgc tgaccgcttg cgaaaacgga caaagcaaag ctgagaactt tattgtgtcg
     1801 gttcaaatgc ctcagcgcgg tctttcgcag gggcagaact ttgtgctgac cctgaccctg
     1861 cagaacaaca gtataaagaa caaagaagtc gaatcgattg tcttacccgc tgcgtttatc
     1921 aaaggggtga acgcgttcaa cacgcagcca gctatgccac gtggcattaa tagcaacggc
     1981 gattgggtct ttgaatatga gcgaactatc gcgccccaag gccttgaaca agtcgctttt
     2041 tactttgaaa ccgcgccaac gggtcatttt gaagcgacag gcgtggtgaa tacctccgag
     2101 cgttcctatg aatttgcgct tgatttaacg gtgcttggcg ttaatcctgc agactggtca
     2161 ccaggcgcgg caaaacccat ctccgacgat gctgtttatg agccaccata cctaagtgtc
     2221 gtcaaaattg acgcctttgc tgaacaagac ggacaacgaa cgcaggtctg gagcggtaac
     2281 ggagcgatta ttagttcaga tgggcttatt ttaaccagcg ctgacgttgt gctttcggac
     2341 cgttactttc agctcaccga cttgcttgtt tctttgaccg ttgatgccga agccccacct
     2401 gttcccagct atcgtgccag catcgtgcag gctgatgcca agctgggact ggcaatcatc
     2461 aagccacgcg aaactctgga tggaaaagct atcgactaca gcttgcttga cttgcccgtc
     2521 ttagcgctgg gcgattcgcg agtcctggag caagacgctg aattacgggt aacaggttac
     2581 ccacaggctc ccaacacatc agagctgatg agcgaaagaa atttacgact caatgacttc
     2641 ggcgcggaac ttccctttgg cgaaggatca ctctggcatg ctgatagagc gattgagacc
     2701 ggctatcttg gcgcgcccgt gctaaacgaa gccggcgaac tggtggctgt gccgatccat
     2761 aaatcgccga tcacttttga cccgcgcaca gtaacgtgtg agcctgttat cgatggcaat
     2821 cgcgatggac ggattgatgc gcttgacccc tgcccgagaa cgagtctgcc gattgacacc
     2881 ctgcgaccca ttcacctggc ccaggaaatg attgacgctg ccaggcgggg tgagataagc
     2941 atctcaatgg ctggaatcgc gcagccttta agccatacgc cagtaaaact gcttgacacg
     3001 gatgattttt cgaacaatcg caatcaatgg aaaatcaccg ggaatgactt tggcgagcaa
     3061 aagattgaag atggtgccta tctgtttaag ttgcataagc cgatgacaca gctttggtca
     3121 tcagtgggct atggctatga tcagcccatg caaattgacg tggaagcgcg cgtgactgcg
     3181 ccggtgtata atagcgaatt tggcgtgatt tgtggcctga ctgagaccga gcgcaccgtt
     3241 ttcgcggtga gtgagaatgg ttatttcgcg atttggaaag tgcaaaatga cgccatgcat
     3301 tatttgcagg actggcgcta cggtggcttt gatgctgagc aaacgctaaa tctttccgtg
     3361 caatgtgggc agggctggta tcagtttgcc gttaatgagc gtgtgttggc gcagggtacg
     3421 gaccctgatt ttaagcctgg actaacgggt ttatatgtag ccagttatga caagggtggt
     3481 ttgacagttc atttcgataa ttttagcgtt tctatcatcg agtaaaagaa aaacccccgt
     3541 gataagcggg ggtttaagga gaaaggtaat gtctaaagcg cgatgtagcg ttcaatcatc
     3601 ggctttagcg tatccagtga actcttccag aaggctttat cgcggatatt gatgccaaag
     3661 cgggcagcca ggtcggcggc gttggcttcg ccggtgctgg acaggagcgc tttgtaatct
     3721 tccaggaaag catcgccttt ttcgagataa atcgcgtaca aaccttttcc aaacagcgtg
     3781 ccaaaagcgt aggggaagtt gtagaaggaa aggtgggcac tgtagtaatg cggtttccag
     3841 gtccaggcgt atttgttcat cacttcgggg tcaatgccat cgccgtaggt gtcgcgttgc
     3901 gcttgcaaca tgatttctga gatatcatca gctgggatgg tgcctttttc gcgtcggata
     3961 aagacttcca tctcaaacag atagcgcgac atgatatcca cgatggtttg cgcgtcgctg
     4021 gcgattgcgg tttcaagcag catcaattct tcttccggtg atgaaggatt ttttagcagg
     4081 gcggtgagaa caatcgtttc gcacatgatg gatgcggttt cggcgagcgt catgggcgtt
     4141 ctggactgga aaggtgtttt gccagcttga tagatgcaat agttatggaa agcgtgcccg
     4201 agttcgtggg caagggtcat gacctggtca aacgagccgt caaagttgct catgatgcgg
     4261 ctttctttca ccacgggtat gcccatacaa aaggcgccac cacgtttgcc agcgcgttgt
     4321 tcagcgtcaa tccagttgct ttcaaaagcg gtttgggcca gttctgccag ttcgggtgaa
     4381 aactttgcaa agttttccaa aatgagttct ttggcttcgc taaaggaata gtttttgttg
     4441 gcggaaccaa ccggcgcgaa aagactccac cagggcagct tgtcgtgacc cagctttttg
     4501 gctttggcgc ggaagtagtc gcggaacatg ggcagggagt cttgcatggc gcccatcatg
     4561 gcatccagcg tttcgcggtc gatacgtgcc atgtccagcg cgctgtgtag ggcgtcctgg
     4621 cgtccacgtc ggcggttgag cacgttcact tcgcccttga tgccgttcat acaagcggca
     4681 aggggctctt tcatctcttc aaagacttgc atttccagct tgtaagcgcg ttcgcgggtg
     4741 tcggcgtcgg ggtgactgcg caggttgatg agcgcgggca aaggcagggt ctgcattttg
     4801 ccatccagtt caaattcaac actcttttgt gaggtcaaca cgccttgcaa attgtcccag
     4861 gcactgctgg cagaaaggct gagttcgttg gccagttctt cttcggcttc gctcatcata
     4921 tagcggcttt gttcagcttc ttcgagcaag gcaaagctgt gttcatgcgc cgagccagga
     4981 tattgcaagg ctttgggcag acggtcgccc agtttgccca gccaggcgga gatgcggttg
     5041 cttaaattgc cgagtggtag catctcgctt tggactttgg agtataactg ctcggctgtt
     5101 ttgttgaacg agtcaacgct aagcgtcgcg cccaaaaaag cgcctatttt attgccaaca
     5161 aggctaactg tgttgcgggt atcaacaatt tggttaagca actggcttag ggcttcatcg
     5221 ctgatatcag aacttaaagc gggttcaagc ttggtttcgt aaattgcggt gagctccgca
     5281 gcaatagttt tgatgtaatt aatgtcttca acgagttgtg ggtcgtcaag accagtataa
     5341 atgttagaaa gatcccatcg ggttggtgtc atagtctgct cctgttcgtg ttagataatt
     5401 gtattatacc gtgttcttta gagataactt gcctgctgtg caaaatcagc ttaaaaacgg
     5461 tgggtaaaaa acaccgctcc tgtctctaaa gacaggagcg gtggatcttg catcaagttt
     5521 tgttcaaggc aaatcttatc tgcggcgtct gccactgatc aaagacacat aatagagcaa
     5581 ttgcagaact gaggtcacca agccagccac gtaagtccag gctgcggcat tgagaacctt
     5641 attgacgcct ttgcgttctt catcgctggt catcaactgg ctttcttcaa gcatgacttt
     5701 ggcgcgtttt gacgcgtcca gttcaatggg caaagtaacc aacgagaaaa cggtcgtact
     5761 ggcaaacagg ataataccga ttgttgccag tccaagcaag ttcagcacca gtccgatgat
     5821 aaccaaaatc atgccaacgt tggagccgat attgacaacg ggcacgagta cacggcgtag
     5881 tttcatcgga ccataattgt cgcggtcttg cgcggcgtgt ccgagttcat gcgcggtaat
     5941 tgccatggct gccacagagg gcacagcacc aacgttttgt gagagtgcca gggttttact
     6001 cctgggatcg taatggtcgc tgagtgtgcc ttgcgtttgc gtaaagctta tttcatcaag
     6061 attgtaacgg gatttgagat agtcggcgac ctggagaccg gtcaggttac gtgtgttgcg
     6121 cacctggctc catttcttgt aggctgaatt taccgcgata ctggcaatta tcgacagtac
     6181 caggcctggc aaaataaaga taaagtaagt tggactaaaa ggcatcttgg tttatccttt
     6241 caaagcgggc tttatttatt gaagtattca atggcagcat cgcgttgcgg gtcaatgccg
     6301 gcttcaaagt cagcttcggt gtagggcact tcaatgtcag gagtgagacc gatttcagca
     6361 atctgatttc catcaggggt caaccaacgc gcgatggtaa tgcgcacgcc accctggtta
     6421 tcagaaaggt ctatccagct ttgtaccgag cctttaccat agctcacttc gccgatgagt
     6481 ttcgcgcggc catggtcttg cagcgcgccg gcaacgattt cggaagcgga agcagagcca
     6541 ccgttaatca agaccaccat gggaatgttg ggggcaatag cgtcaccttc agcccggtag
     6601 gtatcgcggg tgccatcgcc atattcttcg taaaggataa cgccatcctc gataaacatg
     6661 gcggaaatat caatggcggc ataaagataa ccaccgccgt tatcgcgcag gtcaaagatg
     6721 agtcccttgg gattttgtgc catgagctca ttcaacgcgt ttgaaacttg ctctaccgct
     6781 ttatcgttga aggtgtatag cgcgatgtag gcaatatcgt tttcaagcat ttcgtaatca
     6841 acgactggta tttctattac ccggcgtgta atactaacat caaagatttc atcaccacgg
     6901 cgaatggtta aaacgaccac ttcacccgcg gggccaagaa tcgattgcag aacgatatcg
     6961 ccggcggttc cggttacatc ttcgccattt acagcgatga tgatatcttt gggcttgagt
     7021 ccggcttctt cagcggggga gcctttcatg ggggaaacga tttcaacata ctctccggaa
     7081 gtgttcaccc aggcgccgat gcctgtgtaa gagccttcca tttcggcgtt ggcttgttca
     7141 taagtgtggg ggtccatgta ggatgtgtgt ttgtcgccgg ttgcagccaa catgccctga
     7201 atggcaccac gcatcagttc aacgtcgtcc aggggctggt ccacgtagta tctgtgcaga
     7261 taattccagc tttcccagaa aggcttaaag agcgtttgta actcgcgtgg ggttgacgag
     7321 ctgacgtact cagaacactt gccagcatcc gcgccaaatt gaatggcggg gcaggggtcg
     7381 cattcgagtc cttcagttac accatcacca tcaggacaaa ggcaggcagg acacgggtca
     7441 gaaacaccaa cagtattgcc gagtttgtcg taacccaagg cgatgcggat gggattgaat
     7501 ttcacaaggc tggccgtggc tacgccagca ccataggatg tgacaacgat gagcacaatt
     7561 aagattactg ttaatattgt gcgatttgat ttgttttgca tgcatacctc caaagtcaac
     7621 ttgcctgggc aagtcgctca tatacagacc ttaagaatac catactcgga ctattttcac
     7681 cgctggcttt agcggattaa acggaaatta tacaaaaatt aaacacatta tgggcaatgc
     7741 aggggatggt ttttgagacc ttggaaaagc tagttctagg ctatatttct ataactccta
     7801 tgataagata attaatggga aaattattta caccataata aacgaataaa aagttctaaa
     7861 gtaactatca tcatgtgatg attctaaaaa atacagatca gcaaaggagt agcctatcat
     7921 gaaaaacatg tttaactata taatgacaaa ttatctgaca gctaaaaaag aagattttag
     7981 aaaccatcct gttgcaaact ttattcgcaa taaagcagtt aatattattt cagaaaaagc
     8041 atatatcgat gaaacaaaat ataggataac aggctcagca gggatgggac aatggacaca
     8101 ttttccttgg attgctattt tcgacattga tatcaccaca agtgcgcaaa gaggctttta
     8161 tcctgtttat ttattcacgg aagacatgag gggagtatat ctgtcgctga atcaaggttg
     8221 gacctatttc aaaggcgaat atggaacgag gttaggaatt aaaaatattt ctatagtttc
     8281 taaattctgg aaagaaaaac ttttttcaaa tttatctgat ttcgaagagc cgaaaatatc
     8341 cctcatcaca caatctttga atcatgagaa tgctaccggg tatgaaagag gtcatataat
     8401 ggggacttat tacgacttga agtctttacc tgatacaaaa aatctagtga atgatttgcg
     8461 aaacctactt ggcgtttata gagaaatgaa aggaataatg gggaataatt ctttcgagtc
     8521 aatggttgat aaagttatta acaaacaaga cagtcttgat aatgaagatg gtttttatag
     8581 tattgaacct gatagaagcc acttagaaat tcgggataaa tcagttttaa ttaccccttc
     8641 gcaagttgac ttcctggaat tgcaaaaaca acaaaaagaa ttaggattaa gtggagaaga
     8701 actgataata aaatatgagc agaataagct tatctctgcc ggaagacaag atttagctca
     8761 aaaagtgctt cacgaatcaa aagaacatgg cgatggtgcg ggatatgaca ttctttcgtt
     8821 taactccaaa ggagacacca tatttattga agtcaaaaca acaacaagag atatccatac
     8881 gaccttcttt ctttctgaaa acgagcgtag attctctgat gaaaacgctt cttcttacaa
     8941 gttgtacaga atctatgatt ataaagaaga aaacgatagt tttgcctttt acatcataga
     9001 agggtcaatg tcgaaaacat tggatcttga accaacagca tattacgtaa agaacattca
     9061 tactaaagtt cagcacgtaa gtagtgaaaa gtaatcgaag tgagctgtta ttgcgtaaaa
     9121 agatgcttta aaagcgtgtt ttgttcaaat attcgggcaa gtggatgaga tgactgatgt
     9181 acggatggtc gccatcgggt tcgggcttaa cctggagggt atgaatacct aactgacggg
     9241 cggtgtccaa attgatgctg cggtcatcca aaaacaggca gttttccggc ttggggttac
     9301 cgatcagatt tagcgcgatt tggtagcttt ccttttctgg cttacaataa ggcgcggtgg
     9361 caacgacgtc gataatgtcc acaaacaagt cgcgcaggtt catcagtccc aaaacatttt
     9421 ccgcgtgggc gacactggca ttggtgtaaa tccatttggg ctggggcaga ctttcgagcg
     9481 ctttgcgcag ggcgggatct ggtgccatat aatccgaaag gtccacggaa tgtacccagt
     9541 tgagatagtc gtgcatatca acaccccgtt caacctgcaa gccacgcaag gtcgtgccgt
     9601 attgcacaaa aagacggtgt tgcagttcag ccgtttcttc aaggggaacc cctaaaactt
     9661 cgtggatata gctttgaatg cgttccataa acaggggcca aatgttgagc gaactgggat
     9721 aaacggtgtc gtcaaggtca ataagcagga tagaaatttg gttcatcatg tgcggtctaa
     9781 ccacctgttc atattttggg gcggggtgaa ttcatccagt ttttgcagaa ggtcgatggg
     9841 cgtatcggcg acgataaaaa gcccgcggtg ttccggatag atgtaaccca gggcgatgct
     9901 gtgttcgacc atgcgcacga agggatcaaa atagccttca atattgagca gaccaactgg
     9961 tttggtgtgc aatccgattt gtgcccaggt gagcgcttca acggtttcgt aataggttcc
    10021 gtatccgccc ggcaaggcga tgatgccatc agccagttcg agcatacgct tttgacgggt
    10081 ttggatatcc ctgaccgttt caagtttggt caaagtggca tggatgagat gttcaaggtt
    10141 gagcgcgtcg tttaccaccc ctatgacctc gccgtggttt tcgagtatgc catttgctac
    10201 cgcgcccatc agtccggttg aaccagcacc atagattaag gtgcggtttt gtttggcgag
    10261 agcttcgcca agttgccagg cgatttgcat gtgggtttcg ggtaaaccat ctgccgagcc
    10321 ggcaaagacg gtgatgtttt tacgaaaggt ggttttcata ctgttctatt gtagcctata
    10381 aactctctgt ctccagcaag aatgtcaaaa tgtgcatatg ttttatgcct tgtgtgctgc
    10441 gattgatcgg atccattgag atgatatatt tcgggtagtt gtcttttatg tccagcaggg
    10501 gcgatatctc gcgattgaag gtagcttcat cgttcatgat atagctaact tgaacataaa
    10561 actttttatc agctttttcc gcgacgaaat caacttcctt ttcgccaatt ctaccaacag
    10621 ttacatcaaa accccgtctt aatagttcca taaaaacaat gttttctaga atgagatgga
    10681 tatccctaaa gttttcgcca taaaccgcct gacgtatgcc atgatcagca aggtaatatt
    10741 tttcatctat ttgcagaatg cgcttaccgc gcaaatcttg tcgttttgca ggataaaaca
    10801 agcaagcgtc cagggtggct ttaatgtagt taatgaccgt ttcgggcgcg accgcgcgat
    10861 attcattttt taagtaggct gaaatgctgc gggctgaaaa tggattgccg atgtttgaca
    10921 tcaaaaacag aataacccgt tgtaacaggt caacatcgcg gatattgtgt cggctaacaa
    10981 tgtccttaac cacaactgac gcgtaaagat cttttaaata ttgttttgaa ggttccacgt
    11041 caaagtcgag atgatgcaaa aacggcattc cgccaagatt gatgtacaaa ttaaaggcgg
    11101 cgtctttatc cagctttaat ggtgtgtgtt caagtaattg tgtgaactct agaaatgaga
    11161 agggataaat ttgaaattcc acatatcggc cagccaggta agttgccagt tctccggata
    11221 ggagcgaact gttggagccg gtgatgtaaa tgtcggaatt aaagtcaacg cgcaagctgt
    11281 taaccgcccg ttcccaattt tggacttcct gaatttcatc caggaaaatg taaacttttt
    11341 tattgcgatc aaacggttga ttttgaagtt cgtggattaa gtcctgatcg tcgcggatat
    11401 gcatggcttt tgcggattca aagttgagcg tgaagatttg agagggcgca ataccttgca
    11461 tgataaggcg gtcctggatt tgttcaagca aaacagactt tccgcttctg cgcaatccag
    11521 taagcacttt tataatcggt ttgtcaataa a
//
LOCUS       DAOSFC010000010        11488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_4736_length_11488_cov_5.450101, whole genome shotgun sequence.
ACCESSION   DAOSFC010000010 DAOSFC010000000
VERSION     DAOSFC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11488
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4736_length_11488_cov_5.450101"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..446
                     /locus_tag="PK791_00520"
     CDS             24..446
                     /locus_tag="PK791_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91829.1"
                     /translation="MRYTLSPEWDIMVPDHFQHRVEGEHAVFWTRGVTLLCTVFSYSG
                     EKGRQTLLANLRARAEIRELQTIEDIQGEIVRFAYMQTEELRPGHHRLALHAFTTAPY
                     GCLQTSFYMDDPATLQDQLKIWNSSRYHAEGTPPDIAD"
     gene            476..1294
                     /locus_tag="PK791_00525"
     CDS             476..1294
                     /locus_tag="PK791_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91830.1"
                     /translation="MPQIQPEIGFIGFFDILGYSQIMLNNNIHKTSQFVSDTLMNIPK
                     DLISRLRNIHANDNNQQMGHPFDAEEWQRMLDKVQWIIFSDSILISMPMNPEMDALSQ
                     TNLYLAFSTVCASLLNQTFSAGFPLRGGISLGEFFIEDRCFAGRPIINAYRVAQDLEM
                     SGCILDESADAFVSDLRRALVQNQQTEALAKLDQTTILYIAPTKLGEDERFRAINWVT
                     LESPGFHAFRDDVRDATMKAFLMHNKIAVPSVQGKIVNTEMFIRHVLTNLQEWL"
     gene            1460..2149
                     /locus_tag="PK791_00530"
     CDS             1460..2149
                     /locus_tag="PK791_00530"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOH91831.1"
                     /translation="MPYLTYNYELFLIGALALLFLFRAVRAMIYKEINIPRETVIVAL
                     ALFIAFLSTQTFENHAIRIDGFAPRYNLTLFKTIGKLIEKGRSYDGQETYHLHINIIY
                     INLLGNLLIFAPVGFLSAFLFKEAKWYKSLLAGFSLSFTIEIVQLFLITRSFDIDDLF
                     LNSLGTLIGYLFFKLVTLIPPVKRFAQKTGHSERPYGWQWAIAYILCVILLSLAIFLH
                     QYRIAKSVPWG"
     gene            2225..3205
                     /locus_tag="PK791_00535"
     CDS             2225..3205
                     /locus_tag="PK791_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91832.1"
                     /translation="MKKTYLLSITLIIIAMLGLAACKTATPYPDVDPASQTQVAIFAQ
                     ATLTKYAEMQPTQEPAEVETPMPTFTLIPEESEVPGEESTEAPAEVTEAPTEAPTEAP
                     TAEPTAVPTAIPQPTSPADLPQPPSGHTRISFDTGTTNKTVTDTVEANQTKRYVLWMA
                     KWQIMDISTSADNAAYISVKTPSGKVLVGFPNRWIWYRDFAQESGDYVIEVSGMAYKS
                     NFNLKVSVPQILQFEKGASSLKALATVPANGSHEFSAWANQGQTMRLNLNNSEKFILS
                     VMHADGTEVLRYDAKLASAEFVLPKAGTYIIKVHNISSEALSVELSLEVK"
     gene            complement(3289..4122)
                     /locus_tag="PK791_00540"
     CDS             complement(3289..4122)
                     /locus_tag="PK791_00540"
                     /inference="COORDINATES: protein motif:HMM:NF017517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HOH91833.1"
                     /translation="MIFHYTTQAEWDAAQESGVYLPKNFADDGFIHCSDNYQLRFIAN
                     RLFLDTPNLICLAINADAVKEHIVYENLEGGEMPFPHIYGPLPVSAVEKVYVMQKDES
                     GKIILPDELASGPVPLITLLPVHLPGTLYRSPTPGSWMFDPDDEIFDKYLKAGINVVV
                     VLSPADELKRNTGRDLFKRYADAGIEVIHAPTADFSAPEKGYWDGTIAQAIQHLNEGK
                     NLAVHCHAGLGRAGIFIACMVQDILGLNPEQAVEWTRQYIPRAVDTYLQRQFVRDYKK
                     A"
     gene            4419..4913
                     /locus_tag="PK791_00545"
     CDS             4419..4913
                     /locus_tag="PK791_00545"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOH91834.1"
                     /translation="MKVKVDQDLCIACGICEGDLPEVFHIGDSGFAEVIMCPVEADFH
                     DMLQQVAEACPTAAIQIKEAKCSEQKAETPKGACRGADASKPIEDVSTNTNPERNVNM
                     KIKVVEDLCIGCGICEGIAPEVFSLETEPYAVVLLEAIPEELQADVREAADACPEAAI
                     EIEE"
     gene            5032..6261
                     /locus_tag="PK791_00550"
     CDS             5032..6261
                     /locus_tag="PK791_00550"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH91835.1"
                     /translation="MNISNISDTVNGQKRILILTSDAGFGHRRSAESVEKALIKHYGD
                     QVDARVVNPITDTDKALLLNNVEKSYNTGIKNTPALYRLGYQLSDNRQVSKMMDGALT
                     LALTSSLKEVISTFKPQAILNTNPMFNSGVGFALDQLKMHMPYFTVVTDFADVHALWF
                     SNEPDVYFLASDWVRVRALENEMKPQKLIVSGIPVDPIYEEPVASKAKLRKKLRIEPN
                     LPTLLIVGGERVKNVVETVEALENLEHDFQVVAVAGGNDRLFLDLVKLKPKFPLHVHH
                     FLSNMPEWLRAADVLISKAGGLIISEGLAAGLPILIIHYLPGQEEGNVRYVLSHQAGA
                     LPANTGEAVATLDYWLENDGYALKLAAQAAKYLGRPHAASEVAQALWESIDDQTQSPT
                     SANADFVESVAETPDEE"
     gene            6274..6894
                     /locus_tag="PK791_00555"
     CDS             6274..6894
                     /locus_tag="PK791_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91836.1"
                     /translation="MQPESTSPEHVSYASQAAEACPSQPLTKKSKTLSAIMLAIITVA
                     GIALLIWFDLDEIGAFMHANASWVVLISQVIFIALGFTIIPATPICLILAAVMGPWKA
                     IFWGTVGMTLSALVEYFVARQFHDAFDVDRWRAKLPPRWRNLPLDSILVLIFGRFFPG
                     PKVIAFCAVSTRTPMFKYIWTAFVSNLLGMAIVILPFVGILKLPWA"
     gene            complement(7042..7470)
                     /locus_tag="PK791_00560"
     CDS             complement(7042..7470)
                     /locus_tag="PK791_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOH91837.1"
                     /translation="MTQVFVFSDHEPLRILEIDERADQITARVNQGLWESYLGYEAYP
                     SQSWQARQVGKAVLLTNPQPAEVFQGFKLDARDFQILQALISGLNVDQIAWYLHISTR
                     TVRARLKKMQVQFRVESLYALIALVTAMGLIFPDVGAIYD"
     gene            complement(7579..8802)
                     /locus_tag="PK791_00565"
     CDS             complement(7579..8802)
                     /locus_tag="PK791_00565"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH91838.1"
                     /translation="MQNDSHPQTKVRYSSQFWLMFVGLVISSTGTTMIWPFLTVYASS
                     KLSMPMAAIMGLMSFNSVAGLVSSILAGSLVDRFGRKGVMTVGLVGAAAVYAGYIFAT
                     QYWHFVLLLIFAGFFSPLYRVGTNVTVADVVEAEHRAHAYSITRMGHNIGVALGPILG
                     GFVLAKSYNIGFISASLALLVYGAIVVIFIKETMPTQNARESLREQFLIYKEALKNLR
                     FSWMIVSHTLMEICSTFMWVMLAVHMKNNFAYNEDVYRWLPTTNALMVILLQVVITRF
                     VQRWRDTKVMILGAMFYPISMLIIAFSNHFWGFWLSMVVLTMGELIVSPTASAYVANL
                     AEPEKRGRYLGMFSLTWPLAMAVGPVTGGFLSDKIGITAPWLAAAVVGVLAVLSFWGL
                     DRYSQKKRLQDILSK"
     gene            complement(8858..9415)
                     /locus_tag="PK791_00570"
     CDS             complement(8858..9415)
                     /locus_tag="PK791_00570"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HOH91839.1"
                     /translation="MHLRHRLGLFALAGLLLCGACSYQAKALPLSPWVDENLPFTISL
                     PENWELIALASDNNTQYRIAPDDYSEGDESFRVYFYFSETQSENRDEILEESSQKLEN
                     WMDELIDDDYEIYNKSELKIDKKPAVGLDFAKPVDDSYLVGRVVIGTHKTHTIAFVAM
                     ADKASWDANVRTFDKIIRSLKIKSD"
     gene            9454..10149
                     /locus_tag="PK791_00575"
     CDS             9454..10149
                     /locus_tag="PK791_00575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOH91840.1"
                     /translation="MPTKNGASSAPLEYKIIYSKRKTIELRFDGANLLLIKVPQRTRQ
                     SAIEAILHQKADWIERTRKRLAQRLPVPQMVQGEQILLLGNWHSLNIQKVVTPDEVFN
                     TPGVITLSQKLASQGVAGLIPLYRQVTRQWTEHYAQIYSEKHQLPYNGIRVSAAKTRW
                     GSCGANNTLNFSWRLAMTPLLSIEYVVAHELAHIKHRNHSKAFWGFVAQIMPNYEQGR
                     AWLKQNGHALPMF"
     gene            10249..10998
                     /locus_tag="PK791_00580"
     CDS             10249..10998
                     /locus_tag="PK791_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOH91841.1"
                     /translation="MNIAIIEDQKAQREALYQATRDWLRANAPTGRVMAYQSAESFLF
                     AWEEESFNLLLVDIQLPGESGIALVERLRAKGHETMVVFITGVKDFVFQGYKVNALDY
                     LLKPVSIKDLYAVLEKAQARLARQSPRLLLEQGGVLSEIAIETIHYIEVLGHTSTLFY
                     NRPGNPPDVALLEYSSRNSLQNWLNRLEEVAGPGVFISPHRSFIVHLGWVSRISRDEL
                     KMADGFIVPIARGRWQEVSKAWLAYRRGHSA"
     gene            10995..>11488
                     /locus_tag="PK791_00585"
     CDS             10995..>11488
                     /locus_tag="PK791_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91842.1"
                     /translation="MNLLVLLLFCLPVYVVFRLARFKVNWANVLILALILAGFLGLAV
                     WLARPWILFAGGGLTLFFFVLFYDDEPFRIELRWLLPVLLITPCFALLLPMRFAFGAS
                     FLFALFFIWAWKRALTWPRALLVALLFVALSFVSPLYLQIILSGLSFIALDGLMYSIQ
                     KHFD"
BASE COUNT         2821 a         2892 c         2907 g         2868 t
ORIGIN      
        1 attagatagg ctataattcc actatgcgat acacgctttc tcccgaatgg gacatcatgg
       61 tgcccgacca ttttcaacac cgcgtggaag gtgaacacgc cgtcttttgg acgcggggcg
      121 tgaccttgct ttgcacggtt ttttcgtatt ccggcgaaaa agggcgccaa acgcttttgg
      181 ccaaccttcg cgcgcgggcg gaaatcaggg aattacaaac gattgaggat attcaaggcg
      241 agattgtgcg ctttgcttat atgcaaaccg aagaactgcg cccgggacat caccgcctgg
      301 cgttgcatgc ttttaccacc gcgccatacg gctgtttgca gacctcgttt tacatggacg
      361 acccggctac acttcaagac cagctcaaaa tttggaactc aagccgctat cacgccgaag
      421 gcacgccacc tgatatcgca gactgaacgc tggcacaaac agaaaggaga ctaacatgcc
      481 ccaaatacaa cccgaaatcg gctttattgg ctttttcgat attcttggct acagccagat
      541 tatgctcaac aacaatattc ataaaacatc tcagtttgtg tcggatacct tgatgaatat
      601 ccccaaggat ttgattagcc ggctgagaaa catccatgca aacgacaata atcaacaaat
      661 gggacatcct tttgacgctg aagaatggca gcgcatgctg gataaagttc agtggattat
      721 cttctcggat tccattttga tttcaatgcc catgaacccc gaaatggatg cgcttagcca
      781 aaccaacctt tatctggcgt tttcaacggt gtgtgcttca ttgctcaacc aaactttttc
      841 agctggtttc ccactaaggg gcggtatttc actgggcgag ttctttattg aagaccgttg
      901 ttttgccggc cggccgatta tcaatgccta tcgtgtggcg caagacctgg aaatgtccgg
      961 ttgtattttg gacgaaagcg cggacgcctt tgtaagcgac ttgcggcgcg cgctcgtgca
     1021 aaatcagcaa accgaagcgc tggcgaagtt ggaccagacc acaattttgt acatcgcgcc
     1081 gaccaaactc ggtgaagatg aacgctttag ggcgatcaac tgggtgacgc tggaaagccc
     1141 gggctttcat gcttttcgtg acgacgtgcg cgacgcgacg atgaaggcgt ttttgatgca
     1201 taacaaaatc gctgtgccgt cggtgcaggg caagattgtc aacacggaga tgttcatcag
     1261 gcatgttttg acgaacttgc aggagtggtt ataagctttt tttagtacat ggcgatgacg
     1321 ccatgtatca gtaaatgacc aaagacagga aagttggtag cttccttgct tatttaatgt
     1381 acttcgcaat agatttccag gcgggactaa caaaatcagg ctaaaaaatg acctgttcaa
     1441 tatcggttac aatgcaacta tgccgtactt aacctataac tacgaacttt tcctaatcgg
     1501 cgcgttggct ttgctctttc tctttcgcgc tgttcgggcc atgatataca aagaaatcaa
     1561 tatcccgcgc gagacagtca tcgtcgcgct ggctcttttt atcgcttttc tcagcacgca
     1621 gacttttgaa aaccacgcta ttcgtataga cggtttcgcc ccacgctata atctaaccct
     1681 tttcaaaacg ataggcaagc ttattgaaaa aggtcgtagc tatgacgggc aggaaaccta
     1741 tcacctgcac atcaatatca tttatatcaa cttgcttggc aacctgctca tcttcgcgcc
     1801 ggtcggtttt ttgtccgctt tcctgttcaa agaagcaaaa tggtacaaaa gcctgctggc
     1861 tggctttagc ttgtcattca ccattgagat tgtgcaactc tttttaatca cccgtagttt
     1921 cgatattgac gacttgtttc tgaacagtct gggcacgctt atcggttatc ttttcttcaa
     1981 gctcgttacg ctcatcccgc ctgtcaagcg ctttgcccaa aaaacaggtc attccgaacg
     2041 cccttatggc tggcaatggg caatcgctta cattctttgc gtgattctat tgagtctggc
     2101 aatattcttg caccaatacc gtatagctaa aagcgtgccc tggggttgat gggaacatct
     2161 tcgccctgag tcacgtttta acaacgataa accatgcgtt taacgcctta gtctgtgagg
     2221 aaatatgaag aagacctatt tacttagcat aacacttatt atcattgcga tgcttggtct
     2281 tgcggcttgc aagactgcca cgccttatcc tgatgtagac cccgcgtctc aaactcaggt
     2341 ggctattttc gcgcaagcga cgctgaccaa atacgccgag atgcaaccca cccaggaacc
     2401 cgctgaagtg gaaacgccta tgccaacttt cacgctcatc cctgaagaaa gcgaagtgcc
     2461 cggcgaagaa agcaccgaag caccagccga agtgactgaa gcccctaccg aagcgccaac
     2521 cgaagctccc accgcggaac ccactgctgt gccgactgcc attccccagc ccaccagtcc
     2581 ggcagatttg ccccagcctc cgtccgggca cacgcgcatc agctttgata ccggcacgac
     2641 caataaaacc gtcaccgata ctgtggaagc caatcagacc aagcgctacg tgctttggat
     2701 ggcgaagtgg caaattatgg atatcagcac gtcagcagac aacgcggcat atatttcggt
     2761 gaaaacgccc agtggcaagg tgctggttgg tttcccgaac cgctggattt ggtaccgcga
     2821 tttcgcccag gagagtggcg attacgtcat cgaagtttct ggcatggcat acaagagtaa
     2881 tttcaatctc aaagtcagcg ttccgcaaat acttcagttt gaaaagggcg cttccagcct
     2941 gaaagcgctt gccacagtgc ccgcgaatgg cagccatgaa ttttctgcct gggccaatca
     3001 gggacaaacc atgcgtctga acctgaacaa cagcgaaaaa tttattctct ctgtgatgca
     3061 cgccgatggt accgaagttc tgcgctacga cgcgaaactt gccagcgcgg aatttgtttt
     3121 gcccaaggct ggtacctaca tcatcaaggt gcataacatt tcgtccgagg cgctcagcgt
     3181 tgaactcagc cttgaagtca aataaacaaa acttacttat ttaaagtaaa actgcccctt
     3241 ttgatggggc agttttgtta tggatagttt attgaacagg caaggacgct aagctttttt
     3301 ataatcgcgc acgaattgtc tttgcagata ggtatcaaca gcgcggggga tatactggcg
     3361 ggtccattcc accgcctgct cgggattcag acctaaaatg tcttgtacca tgcaagcgat
     3421 aaaaataccc gccctaccca aaccagcgtg gcagtgtacg gcaaggtttt tgccttcatt
     3481 caaatgctga atggcctggg cgatggtgcc gtcccagtag cctttttccg gcgcggaaaa
     3541 gtccgcggtg ggcgcgtgga tgacttcgat acccgcgtcc gcgtagcgtt taaaaaggtc
     3601 ccgtccggta ttgcgtttta gctcatctgc ggggctcagg accaccacga cgttaatccc
     3661 ggctttcaga tacttgtcaa aaatttcgtc atcagggtca aacatccaac tgcctggcgt
     3721 gggggatcgg taaagcgtgc cgggtaaatg gactggcaac agggttatca agggcacagg
     3781 gccggaagcc aactcgtcag ggagaatgat tttgccactt tcgtcctttt gcatgacata
     3841 tactttctcc accgcgctca ccggcaaggg gccatagatg tgcgggaagg gcatctcgcc
     3901 accttcgaga ttctcataaa caatgtgctc tttcaccgcg tcggcgttta tagccaggca
     3961 gattaaattt ggtgtgtcca aaaagagtct gttcgcgata aagcgcagct ggtagttatc
     4021 agaacaatga ataaagccat catcggcaaa gtttttgggc aaatacacgc cactttcctg
     4081 ggcggcatcc cactcggctt gggtggtgta gtggaaaatc atagttcaca ctccttaaat
     4141 gtcgtgccta agttttatca ggatttggca ataattgcca tagctgtagc atgcaaagtg
     4201 tttggtcgat ttggcaagtt ctgcgcggcg ggacgccggc aggagaggta ttaagtatta
     4261 tcctcgttca aacgtggtct cctgaccacg ttttttatta tgctcgcccc ggcgtctcgc
     4321 cgcgggcgaa cccgaaagac cgtaactttt aataaagaat aaaccccgct ttgcatcagt
     4381 tcagtgtata ataaggacgt tctggaaaag gagtgtgcat gaaagtcaag gttgaccagg
     4441 atttatgtat cgcttgtggc atttgcgaag gggatttgcc cgaagtcttt cacatcggtg
     4501 actccggctt tgccgaagtc atcatgtgcc cggttgaagc tgattttcac gacatgctgc
     4561 aacaagtggc agaagcttgc cccactgccg caattcaaat caaggaagct aaatgctcag
     4621 aacagaaagc ggaaacgcca aagggagctt gccgtggcgc agatgccagc aaacccattg
     4681 aggacgtatc aaccaataca aaccccgaaa ggaatgtgaa tatgaaaatt aaagttgtcg
     4741 aagatctgtg catcggttgt ggtatctgcg aaggtatcgc gcctgaggtt tttagccttg
     4801 aaaccgaacc ctatgctgtt gttcttttgg aagcaatccc cgaagaatta caggcggacg
     4861 ttcgcgaagc ggctgatgcc tgcccagaag ctgccatcga aatcgaagag taattaattt
     4921 cctgtcaaac gtcagggcac accgcacccg cggtgtgcct ttgcgtatta atacgctcat
     4981 tctaatcagc tcataaacac gctatgttag cttttcagat ataataagag catgaatata
     5041 tcgaatatat ctgataccgt aaacggacaa aagcgcattc tcattcttac cagcgatgcc
     5101 ggttttgggc atcggcgcag cgcggaatcc gttgaaaaag ctctgatcaa acactatggc
     5161 gaccaggtgg atgcgcgggt ggttaacccc attaccgata cggataaagc cttgttgctg
     5221 aataacgttg agaaaagcta caataccggc ataaaaaaca ctccagccct ttatcggctc
     5281 ggttatcagc taagcgacaa tcgtcaggtt agcaaaatga tggacggcgc cttgacgctc
     5341 gccctgactt ccagcctaaa agaagtcatc agcactttca agccccaggc cattctcaac
     5401 accaacccga tgttcaactc cggagttggc ttcgcgcttg accagctcaa aatgcacatg
     5461 ccctacttta ccgtggttac ggacttcgcg gacgtacacg cgctttggtt tagcaatgaa
     5521 cccgacgtct actttttggc gtccgactgg gttcgcgttc gcgcgctcga aaacgagatg
     5581 aaaccccaaa aactgattgt aagtggcatt ccggtcgacc cgatttacga agaacctgtg
     5641 gcaagcaaag ccaagctacg caaaaagctg agaattgaac ccaacctgcc cacgcttttg
     5701 attgtgggtg gcgaacgggt gaagaatgtg gtggaaaccg ttgaagcctt ggaaaatctg
     5761 gaacatgatt ttcaggttgt ggcagtcgct ggtggtaacg accgtctgtt tttggacttg
     5821 gtcaagctta agcccaaatt tcccctgcat gtgcatcact ttttgagtaa tatgcccgaa
     5881 tggttgcgag ccgcggatgt tttgataagc aaagccggcg gattaatcat ctctgaaggg
     5941 ctggcggctg ggctgccgat tctgattatc cattatcttc ccggtcagga agaaggcaac
     6001 gtgcgttatg tgctcagcca ccaggctggc gcgctgcctg ccaacacagg cgaggcggtt
     6061 gctactctgg attactggct tgaaaatgac ggctatgcgc ttaaactcgc cgcgcaggct
     6121 gccaaatatc tgggccggcc ccacgcggcc agcgaggtgg cgcaagccct ttgggaatcc
     6181 atcgatgatc aaacgcaaag cccgacatcg gcaaacgcgg atttcgttga atcggttgct
     6241 gaaacacctg acgaagaata aatcgaatga gtcatgcaac ccgagagtac aagccccgaa
     6301 cacgttagct atgcaagcca agccgctgaa gcctgcccaa gccaaccgct gacaaaaaag
     6361 agtaaaacct tgagcgccat tatgctagcc attattactg tggctggcat cgctttgctt
     6421 atctggtttg accttgatga gataggcgct tttatgcatg ccaacgccag ctgggtggtg
     6481 ctgattagcc aggttatttt tatcgctttg ggttttacca tcatccccgc gacgccgatt
     6541 tgtcttatcc tggcggcagt gatgggcccc tggaaagcaa tattttgggg cacggttggc
     6601 atgacgcttt ccgcgctggt ggagtatttt gttgcccgtc agtttcacga tgcctttgat
     6661 gttgatcgtt ggcgcgcgaa actgccacca cgctggcgta atttgccttt ggattcgatt
     6721 ttagtactta tctttggtcg tttttttcca ggcccgaagg tgattgcctt ttgcgctgtt
     6781 agcacgcgca cgccgatgtt taagtatatc tggacggcgt ttgtatctaa tttgttgggg
     6841 atggctatcg ttatcctgcc ttttgtgggt attctcaaac tgccctgggc ttagaacgat
     6901 tacagctact tgcctgtttt tttataacag tgaacaattc agtatggatt taaattatca
     6961 ccctcctggg gctgcttgcg tgccgggtgc ttttctcggc tgggggataa aagggggtgg
     7021 gcacagggct tttaatttca cctaatcgta aattgccccg acgtcgggga agataagtcc
     7081 catcgcggtg accagggcaa tcagcgcgta aagcgattca acccgaaact gaacttgcat
     7141 cttttttagc ctcgcgcgca cggtgcgggt gctgatgtgc aaataccagg cgatttggtc
     7201 gacgtttaag ccggaaatga gtgcctgaag aatctgaaaa tcgcgcgcgt cgagtttgaa
     7261 gccctgaaaa acttccgctg gctgggggtt ggtaagcaaa accgccttgc ccacctgacg
     7321 cgcctgccag gactggctgg ggtaggcttc gtaacccagg tatgactccc aaagcccctg
     7381 gttgacacgc gcggtaattt ggtcggcgcg ctcgtcgatt tccaaaatgc gtaagggctc
     7441 atggtcgctg aaaacaaaaa cttgtgtcac ggctttcctt atcctggaaa aatagaactt
     7501 acgttctata ttatcgccaa aagcggaata aaagtcaagt caaaagggct acacatttag
     7561 cgcaatcgcg ccaacagatt atttgcttag gatgtcttgc aggcgttttt tctgactgta
     7621 gcggtcaagc ccccaaaacg ataagaccgc cagcacaccc accacagcgg cagcgagcca
     7681 gggcgcggtg atgccgattt tgtcactcaa aaacccgccg gtcacaggtc ccaccgccat
     7741 tgccaaaggc caggtgagtg aaaacatgcc caaatagcgt ccgcgttttt ccggttcagc
     7801 caggttggct acataagccg aagcggttgg cgaaacgatg agttcgccca tggtcagcac
     7861 gaccatactc agccaaaagc cccaaaaatg gttcgaaaaa gcgataatca gcatcgaaat
     7921 gggataaaac atcgccccca aaatcatcac ctttgtatcg cgccagcgtt gcacaaaacg
     7981 ggtaatgacg acctgaagca ggataaccat caaagcgttg gtggtcggca gccagcgata
     8041 aacgtcttcg ttgtaagcga aattgttttt catgtgcacc gccagcatca cccacatgaa
     8101 ggtggaacaa atttccatca gcgtgtgcga aacaatcatc cagctaaagc gcaggttttt
     8161 cagggcttct ttgtaaatca aaaactgttc gcgcaaactt tcccgcgcgt tttgtgtggg
     8221 catggtctct ttgataaaaa taacaacgat agcgccataa accagcagcg cgagtgatgc
     8281 cgagatgaag cctatattgt aagacttcgc caaaacaaat ccacccagga tgggtcccag
     8341 ggcgacaccg atgttgtgcc ccatgcgcgt gatactgtaa gcgtgagcgc gatgttccgc
     8401 ttcgaccaca tctgccacgg tcacgtttgt gcctacccga taaagcgggc tgaaaaatcc
     8461 cgcgaagatc agcaaaagca caaaatgcca gtactgcgtg gcaaaaatat agccagcata
     8521 gaccgccgcc gcgcccacca ggcctaccgt cataacgcct ttgcgcccaa agcggtcaac
     8581 cagggagccg gccaaaatgg acgagaccaa acccgctacc gagttgaaac tcatcagtcc
     8641 cataatcgcc gccatgggca tgctcagttt gctgctggcg taaacggtga gaaaaggcca
     8701 aatcatggtc gtgccggtgg atgaaattac caggcctaca aacatgagcc aaaactggct
     8761 tgaataacgg actttggttt gcggatggga gtcgttttgc ataggcgctg ttactgtctt
     8821 tccttgcttt gaacgcggtt gtcaggcttg gtgctgctta atcgctcttg attttcaaac
     8881 tgcggataat tttatcaaaa gtacgtacgt ttgcgtccca ggaggcctta tccgccattg
     8941 ccacaaaagc gatggtgtgg gtcttgtgtg tgccgatgac cacccggcct accaggtagc
     9001 tgtcgtcaac cggtttggcg aaatcaagcc ccacggcagg ttttttatcg attttcagtt
     9061 cgcttttatt gtagatttcg tagtcgtcgt ctattagctc gtccatccag ttttcaagct
     9121 tttgggaact ttcttctaga atttcatcgc ggttttcaga ctgggtttca gaaaaataga
     9181 aatagacgcg aaagctttca tcaccttcgc tgtaatcatc aggcgcgatg cgatactgcg
     9241 tgttgttgtc actggctaaa gcgataagct cccagttttc cggcaaggag atagtaaagg
     9301 gcaggttctc atccacccac gggctgagtg gcagggcttt ggcctggtag ctgcaagccc
     9361 cacacaggag caaacctgcc agcgcgaata atccaagacg atgtcgtaaa tgcatgcctg
     9421 cattgtaccc gaaaataaga ttataatgag ttcatgccca cgaaaaacgg tgccagttcc
     9481 gcgcccctgg agtataaaat tatctatagc aagcgtaaga ccatcgagtt gcgttttgat
     9541 ggggctaatc ttttgctcat caaagtgccc caacgcaccc gccagtcagc gattgaagcc
     9601 attttgcacc aaaaagcgga ctggattgag cgcacgcgca agcgactcgc ccagcgtttg
     9661 ccggtgccac agatggtgca aggcgagcaa attttgcttt tgggcaactg gcacagtttg
     9721 aatattcaaa aagtggtgac acccgatgag gtttttaaca cgcccggagt gataacgctc
     9781 agccaaaagc tcgccagcca gggcgttgcc gggctcattc cgctttaccg gcaagtgacg
     9841 cgccagtgga ctgaacatta cgcgcaaatt tacagcgaga aacaccagct gccctacaat
     9901 ggcattcgcg ttagcgctgc taaaacgcgc tggggttcgt gcggggcgaa caacacgctg
     9961 aacttttcat ggcgtctggc gatgacgccg ttgctgtcca tcgagtatgt cgtagcgcac
    10021 gaactggcgc acatcaaaca ccgcaatcac agcaaggcat tttggggttt cgtggcgcaa
    10081 atcatgccga attacgagca aggccgcgcc tggctcaaac aaaacgggca tgctctgccc
    10141 atgttttgag tgggaaaaat cgtgtggctt tccctgacca caggcaaagt gccaaccaca
    10201 aaatgccacg aacaagtcaa tctgtgagta caatatagtc aaacagctat gaacatcgcg
    10261 attattgaag accaaaaggc acagcgcgaa gcgttgtatc aagccacccg ggactggctg
    10321 cgagccaacg cgcctacggg gcgggttatg gcatatcaga gcgcggaaag ttttcttttc
    10381 gcctgggaag aggaatcttt taacctctta ctggttgata ttcagttgcc cggcgagagt
    10441 ggcatcgcgc tggtagagcg cctgcgcgcc aagggtcacg aaacaatggt ggtttttatc
    10501 acgggggtta aggattttgt cttccagggc tataaggtca acgcgctgga ctacctgttg
    10561 aaaccagtca gcatcaagga cctgtatgcc gtgctggaaa aggcgcaggc caggctggcg
    10621 cggcagtcgc cccgtttgct tttggagcaa ggcggggtgc tgagtgagat agccattgag
    10681 acaatccatt atattgaagt gctcgggcat accagcacgc tgttttataa ccgtcccggc
    10741 aacccgcctg atgtggctct tttggagtat tccagtcgca acagtctgca aaactggctg
    10801 aaccgcctgg aagaagtcgc tggaccgggc gtatttattt ccccgcaccg cagttttatt
    10861 gtgcatttgg gttgggtgtc gcgcatcagc cgtgatgaac tcaaaatggc agatggcttc
    10921 atcgtgccta tcgcgcgcgg tcgctggcag gaggtttcaa aggcgtggct ggcgtaccga
    10981 cgggggcata gcgcatgaac ctgttggtct tactgctttt ttgtttaccg gtttatgtgg
    11041 ttttccggct ggcgcggttc aaggtaaact gggcgaatgt gttgattctg gcgcttatcc
    11101 tggcgggatt tttgggtttg gcggtttggc ttgcaagacc ctggatattg ttcgccggtg
    11161 gcgggctgac gcttttcttc tttgtgcttt tttatgacga tgagccattc cgcatcgaat
    11221 tgcgctggct tttgcctgtt ttgctgataa cgccctgctt tgccttgctg ttaccgatgc
    11281 gctttgcttt tggcgcgagc tttttattcg cgctgttttt tatatgggca tggaaacgcg
    11341 cactcacctg gccacgcgcg ctcctggtcg cgcttctctt tgtcgctttg agttttgtaa
    11401 gcccgcttta tttgcagatt atcctgagcg gtcttagttt tatcgcgctg gatggtttga
    11461 tgtattccat ccaaaagcat tttgacat
//
LOCUS       DAOSFC010000011        11462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_4761_length_11462_cov_5.125186, whole genome shotgun sequence.
ACCESSION   DAOSFC010000011 DAOSFC010000000
VERSION     DAOSFC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4761_length_11462_cov_5.125186"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PK791_00590"
     CDS             <1..614
                     /locus_tag="PK791_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOH91843.1"
                     /translation="SLTTRELAKILKEGSVDLRYIEEEDFDSPLGVSTGAGVIFGSTG
                     GVLEAALRSVYELVTKETLENVDFVALRGLDGAHEAVVDLGVRKIRVAAVSGLGNARK
                     ILDKVVSGEEVFDVIEIMACPGGCINGGGQPYAKDRDAVLQKRMESLYKLDREDKLRK
                     SHENPEVIALYKDFLGEPGGHKAHDLLHVENFEASVMKEWEQF"
     gene            858..1658
                     /locus_tag="PK791_00595"
     CDS             858..1658
                     /locus_tag="PK791_00595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH91844.1"
                     /translation="MKIDFEETTDDLAKRINIHEQYGSRDIDAWMLDVLQLQPDSKIL
                     DIGCGAGKQCFSYFHYLNGDADITGTDVSRDLLNEAIEKNKQTHNAIKFQLMDFNLPF
                     LMASNQYDLVSACFAIYYAQDIPWTLAEMHRVLKPGGRLFTTGPMPSNKQLFYDIIKE
                     ATGKAIPPMPGSSRYEPQIYPTMQSLFSKAEQHIFENPLTFTSVEPFMVYTRASMSED
                     RKLWNTLLNTHEEFEQVMQAIQQVAQKRLAKDGVLVMTKVVGGFLATK"
     gene            1671..2312
                     /locus_tag="PK791_00600"
     CDS             1671..2312
                     /locus_tag="PK791_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOH91845.1"
                     /translation="MNNMTFYGKSLLFMGAHPDDIELGCGAFIAGMVGKADIFCYTFS
                     DNQKNPDLLNLVTEHANSMRALGLKENQFELGTFETRRFPDARQEILEKMIALRKKYQ
                     PKIVFVHTAQDLHQDHQIVTQEAMRAFRGTTVLGFDVLRSSYGFFPHFLTEVSEEDLN
                     TKIAALAEYKTYQGRYYFSPEIIRATAIRHGALAERPFAEGFDIIRIVANFSE"
     gene            complement(2334..2624)
                     /locus_tag="PK791_00605"
     CDS             complement(2334..2624)
                     /locus_tag="PK791_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91846.1"
                     /translation="MLRTGHFMRPPEDDQIYNIGDRVEVNCDHEDEEGQRIRGWVRGT
                     VVQVENKLIAVQFKNQKIYLTDGWMVPDNILWFPFDSENVRPWVSPQSRARK"
     gene            complement(2647..3831)
                     /gene="sufD"
                     /locus_tag="PK791_00610"
     CDS             complement(2647..3831)
                     /gene="sufD"
                     /locus_tag="PK791_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOH91847.1"
                     /translation="MTNIDSTKSSIMKNLRWPEHWQANVQQISAWELAQSLSFPGRKN
                     EDWKWINFDHIDLDELTVEKPVITILSSDNQDINASVLKADSKENLLIQPETDAYSAL
                     NAALSEELCYIHIPAKIQKLDPVHVIITLNAHSSAANTRAIIQLDKGAQAKVVFELQS
                     DTHEHLQAFLNFQLEIQLDEGARLELMEVQNLGEHVSYVSREYAHVHRDAHLNWTYVA
                     LGSRVAKSFITVDLLGPGSETLMNGAYFSGSGQVIDIDTQQNHLTAQAHSDLLYKGAA
                     IGSGKAIWEGMIYVDHVAQQTDSYQSNRNLLLDDRAEIKTIPGLEINANDVSCSHGAT
                     VGRLNDDELFYSQSRGIPMREAEKLIVEGFFAEVLAKSNDEALSKKLLEQISEKFDRN
                     HA"
     gene            complement(3835..5250)
                     /gene="sufB"
                     /locus_tag="PK791_00615"
     CDS             complement(3835..5250)
                     /gene="sufB"
                     /locus_tag="PK791_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOH91848.1"
                     /translation="MSDQKNQDFLNELGADYQYGFSDPDIASFRPEKGLTREIVKQIS
                     AFKEEPEWMLNLRLKAYEHYINRPMPGWGPDLSDLDLDNIYYYVRPPEVNQKSWDDVP
                     DTIKNTFDKLGIPEAEQKFLAGVGAQYDSEMVYHSIQENLEKQGVVFLSIENGLKEYP
                     ELFREHFGTVVPYQDNKFSALNTAVWSGGSFVYVPKGVKVDLPLQAYFRLNNPNLGQF
                     ERSLIIADEGSQVHYVEGCTAPIYSEDSLHSGVIEIVVKKNARVRYSTIQNWSTNVYN
                     LVTQRAVVYENGTMEWVDANLGSKVTMKYPSCYLRGEGAHGEVLSVAFAGPGQVQDAG
                     SKMIHFVPNTSSKITSKSISRGNGRSSYRGLVRVANNVDKANSSVVCDALLLDPESVS
                     DTYPTIDIRSSDVNIGHEASVSRIGEEQLFYLNSRGLTDEEATTMIVSGFIEPLVKEL
                     PMEYAIEMNRLIQLQMIGSVG"
     gene            complement(5268..6047)
                     /gene="sufC"
                     /locus_tag="PK791_00620"
     CDS             complement(5268..6047)
                     /gene="sufC"
                     /locus_tag="PK791_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOH91849.1"
                     /translation="MTKIEIKDLHVNIGEKEILKGFNLTVNGGEVHALMGPNGTGKST
                     LAYALMGHPRYEVIKGDILLDGESIMEMSVDERARRGLFLAFQYPVAIPGVTLANFLR
                     SALNAKAKALNPEDKGISLVNFRRLLRSKMDLLKMDYAFGARYLNDGFSGGEKKRAEV
                     LQMAMLEPRIAILDETDSGLDIDALRIVADGVHQLQSDTLGVILITHYQRMLEYMQPQ
                     FVHIMYGGAIVESGGAELALKLEEQGYDWIRDKYPDTRKTG"
     gene            6367..6699
                     /locus_tag="PK791_00625"
     CDS             6367..6699
                     /locus_tag="PK791_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH91850.1"
                     /translation="MSEKKSLKDIIKPKKQEKSLATPEPAPKETLTKREAVRQKLEDL
                     PKEPARTHVVAEGETLSHIALKYYGKATPPYYQHIYQHNLEVIGDNINIIVPGQELVI
                     PDLPDELK"
     gene            6721..7692
                     /locus_tag="PK791_00630"
     CDS             6721..7692
                     /locus_tag="PK791_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOH91851.1"
                     /translation="MTKELDLAQVFKAVSKTLVENQSSLDEADTVNHDHGTNMVNTFK
                     LIEKAVKAKKDGTTTEQLAYASQMLREKSTSGSAMLYADGLENAAKEFEGKTFDREGV
                     GTLLNALMGTPQNQAQEQTPSAPQGNDFLSTLVGGMASSQQTSQGEQEPTFAQVPDLI
                     DALGGLTFTQEPVAQEAEQNQESDQTSAIDLVGSLLGGLGQTQPAKQEEEPKPSGLGG
                     LLGNLLGGGKQQSPASSGVGGLGNLLGSLLGGQQSQPSQQAGQAASTATSLLSAALAY
                     LAATQQGKTPFESILEAVGTATRFSDRQDRTQSGALVAQTLIGLLSK"
     gene            complement(7775..8179)
                     /locus_tag="PK791_00635"
     CDS             complement(7775..8179)
                     /locus_tag="PK791_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOH91852.1"
                     /translation="MAKIIDIEGIGPVYAEKLIAIGIKTVEGLLKAGAYPKGREEIAE
                     QTGISSKLILEWVNHADLYRIKGVGEEYSDLLEEAGVDTVVELATRNPENLYAKIIEV
                     NNEKNLVRRPPSQSMVADWILQAKGMDRAVFY"
     gene            8366..8977
                     /locus_tag="PK791_00640"
     CDS             8366..8977
                     /locus_tag="PK791_00640"
                     /inference="COORDINATES: protein motif:HMM:NF037269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOH91853.1"
                     /translation="MNTKNRILNTLLIKQEASVNDLAEELGVNGISIRHHLKNFEKDG
                     WVVSRESRKGQVGRPKFIYSLSSLGMEQFPSSYVEFSSILVDELKNRLDDEQLNALFK
                     EVGLQFAERNRLERRHRNSPKRLSYVVENLNEQGYIIEKKEIDGEIVFTNYHCPYRYV
                     ASATTNLCCLDKTMLEELCESEVEITQTIAKGDPHCTFIIKNK"
     gene            8974..9321
                     /locus_tag="PK791_00645"
     CDS             8974..9321
                     /locus_tag="PK791_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOH91854.1"
                     /translation="MTLSNKPDWKIDKTHPELVESLVLGLSAVLDPDLGYSVLELGLV
                     RDISLTDKGASVVMILTTPFCPYGPAMLEETRAKVAQVLEKPTTIEYGSEAWKPEMMD
                     PELRDDEWGLLGF"
     gene            9557..9715
                     /locus_tag="PK791_00650"
     CDS             9557..9715
                     /locus_tag="PK791_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOH91855.1"
                     /translation="MDKYECIACGYIYDPELGDPTQDIAPGTPFEDLPEDWVCPDCGV
                     GKDQFEKI"
     gene            9833..10945
                     /gene="ugpC"
                     /locus_tag="PK791_00655"
     CDS             9833..10945
                     /gene="ugpC"
                     /locus_tag="PK791_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOH91856.1"
                     /translation="MASVTYDGVWKKFGDVVAVQDLNLAIEDKEFLVLVGPSGCGKTT
                     ALRCLAGLEEITEGEVYIGDVLVNDIAPKDRDIAMVFQSYALYPHMTVFDNMAFGLKL
                     RKTPKEVINQRVQEAAKTLGIENLLHRKPKELSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVQTRAELSKLHKRLETTFIYVTHDQTEAMTMATRIAVMNKGVLQQLDTP
                     QVLYDHPANLFVAGFIGSPSMNFFDGVIEQREGGIYVNLGSFAVAVPEKDYEALKHLI
                     GRPIIFGIRPEDIHNPTYAPPGIIAEPVNAEVDVIELMGNEIFLYLVNGEHNFIARVD
                     PRTRMDIGDKVEVAFNMDNYHIFDPGLDEENPVAVF"
     gene            complement(11019..>11462)
                     /locus_tag="PK791_00660"
     CDS             complement(11019..>11462)
                     /locus_tag="PK791_00660"
                     /inference="COORDINATES: protein motif:HMM:NF018964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HOH91857.1"
                     /translation="VFNARELEHMIDVKKVVKTSLTVWYALLGFTFAVLMWFVIMGAW
                     KPLRQGLIGGAWLTVGLIVALIIFIAVSFNNLFDRFHQLFFKDGTWLFYESDTLIRLF
                     PLTFWRDAFILVSVLTLLFAGLLLIIFGVISKVRKNKQKKSLATP"
BASE COUNT         3283 a         2840 c         2323 g         3016 t
ORIGIN      
        1 actccttgac cacccgcgaa cttgccaaga tcctcaaaga aggctctgtt gacttacgct
       61 acatcgaaga agaagacttc gactcacctc tcggcgtttc aactggcgct ggtgtcatct
      121 tcggtagcac cggtggtgtt cttgaagccg ctttacgcag cgtctatgaa ctggttacca
      181 aagaaaccct tgaaaacgtc gatttcgtcg ctttgcgtgg ccttgatggt gcccacgaag
      241 ccgttgttga ccttggtgtt cgcaagattc gtgttgcggc cgtttccggt ttaggtaacg
      301 cccgcaagat tttggacaaa gtcgtttctg gcgaagaagt ctttgacgtc atcgaaatca
      361 tggcttgccc cggtggttgt atcaacggtg gcggtcagcc ctacgccaaa gatcgcgacg
      421 ctgtgctcca aaaacgcatg gaatcactct ataaactcga ccgcgaagat aaactgcgca
      481 aatcgcatga gaatcctgaa gtcatcgcgc tctataaaga cttccttggc gaacctggtg
      541 gtcacaaagc acatgatctc ctgcacgtgg aaaactttga agcaagtgtt atgaaagaat
      601 gggagcagtt ttagttccat tcgcttctaa gtaaaattta gtatagaaaa actctccttt
      661 gatattcaag aagagttttt tatttaatga acaaaattga acttagacat ttcaaactac
      721 caagagattt actggcattg acccttacta caccagggaa aaccatcaaa gctgattgtg
      781 caggataacg gagtccagcc ttgacgtact tttttgctga tttatactta agcaataact
      841 gtagacaagg actaattatg aaaattgact ttgaagaaac aaccgacgac cttgccaaac
      901 gcattaacat ccacgaacag tatggcagtc gtgatataga tgcatggatg ctggatgtgc
      961 tccagcttca acccgatagc aaaatcctgg atataggttg tggagctggc aaacaatgtt
     1021 tttcctactt ccattacctg aatggcgatg cggacatcac cggcacagac gtctcgcgtg
     1081 atctgctcaa cgaagcaatc gagaaaaaca aacaaactca taacgcaatc aaattccagt
     1141 taatggattt caacctgccc ttcttaatgg caagtaatca gtatgatttg gtatcagcct
     1201 gtttcgccat ctactacgcc caggacatcc cctggacact tgcagaaatg caccgtgtcc
     1261 tcaaaccagg tggcaggctt tttactaccg gcccaatgcc cagcaacaag caactctttt
     1321 acgacatcat taaagaagcc acaggcaaag ccatcccgcc catgccaggc agttcgcgct
     1381 acgaaccgca aatctacccc accatgcaaa gcctgtttag caaagctgag caacacattt
     1441 tcgaaaaccc gctaacgttc accagcgttg aacccttcat ggtttacacc cgtgcgtcaa
     1501 tgtcggaaga ccgcaaactg tggaacacgc tcttaaatac gcacgaagaa ttcgaacaag
     1561 tcatgcaagc catccagcaa gtcgcccaaa agcgccttgc caaagatggt gttttggtca
     1621 tgactaaagt cgtcggtggt tttttggcaa cgaaatagaa ggaaaacatg atgaacaata
     1681 tgacatttta cggaaagtca cttctcttta tgggcgcgca ccctgatgac attgagttag
     1741 gctgcggggc gtttattgct ggcatggttg gaaaagcgga catcttctgt tacacctttt
     1801 ctgacaatca aaaaaaccct gacctgctca atctggtaac agaacacgcg aacagtatgc
     1861 gtgctttagg cttaaaagag aatcaatttg aactcggcac ctttgaaaca cgtcgttttc
     1921 ctgacgctcg ccaggaaatc cttgaaaaaa tgattgcctt gcgcaagaaa tatcaaccaa
     1981 aaatcgtatt cgtacacacc gcgcaagact tgcatcaaga ccatcaaatc gtcacacagg
     2041 aagcaatgcg ggcttttcgc ggtaccaccg tgttgggatt tgacgttttg cgctcttctt
     2101 atggtttctt cccccatttc ctgaccgaag tcagcgaaga agatttaaat acaaaaatag
     2161 ccgccctggc tgaatataag acttaccaag gccgctatta cttttcacct gaaatcatcc
     2221 gtgccactgc tatccgtcac ggagcacttg ctgaaagacc ttttgctgaa ggtttcgata
     2281 ttattcgaat cgttgcaaat ttttctgagt aggacttaat cgtcccactt gctttactta
     2341 cgcgcgcgac tttggggtga tacccagggt cgcacgtttt ctgaatcaaa aggaaaccac
     2401 aagatattgt ccggaaccat ccatccatct gtcagataaa tcttttggtt tttaaattga
     2461 acagcgatga gtttattctc aacctgaacc accgtaccac gcacccagcc acgtatgcgt
     2521 tggccttctt catcttcatg gtcacaatta acttctacac gatcaccaat gttataaatc
     2581 tggtcatcct ctggtggtcg cataaagtgt cctgttctta acatataagc tcctttctat
     2641 cagccattag gcatgatttc tgtcaaactt ttcagatatt tgttcgagta gttttttgct
     2701 caacgcttca tcgttcgatt tcgccaacac ttccgcaaag aaaccttcca cgattaattt
     2761 ttcggcttct ctcatcggaa taccccggct ctgagaataa aataactcat catcattcaa
     2821 cctgccaact gtcgcgccgt gcgaacagct aacatcgttg gcattaatct ccaagcctgg
     2881 aatagtctta atctccgcgc ggtcatccag caaaagattg cgattgctct gataagagtc
     2941 tgtttgttgg gcaacatgat caacgtaaat catcccttcc caaatcgctt tgccactgcc
     3001 aatcgcagca cctttatata acagatcact atgtgcctgg gcagtgagat gattttgttg
     3061 tgtgtctata tcgattactt gcccactgcc actaaaataa gcgccattca tcagggtttc
     3121 tgaacccggt ccaagtaaat cgaccgtgat aaaacttttt gccacgcgag accccagggc
     3181 aacgtaagtc caattaaggt gcgcgtcacg atgcacatgg gcatattcgc gactgacata
     3241 tgaaacatgc tcacctaaat tttgcacttc catcagctcc agtctggcac cttcatccag
     3301 ttgaatttcg agctgaaagt tcagaaaagc ttgtaagtgc tcatgcgtat cgctttgtaa
     3361 ttcaaacaca acttttgcct gtgcgccttt atcaagctgg ataattgccc gggtattagc
     3421 tgcactgcta tgcgcattca acgtaattat aacatgaact gggtctaatt tctgtatctt
     3481 tgcggggata tgaatatagc aaagttcttc actcaatgca gcgtttaatg cactataagc
     3541 gtccgtttct ggctggatga gtaagttttc tttactatca gccttaagca cactggcatt
     3601 gatatcctga ttatctgatg acagaatcgt aataactggc ttttcaacag taagttcgtc
     3661 caaatcaata tggtcaaaat taatccattt ccagtcttca ttctttcgcc ctggaaaaga
     3721 cagcgattgt gctaactccc acgccgaaat ttgctgcacg ttggcttgcc aatgttctgg
     3781 ccagcgcaaa tttttcatga tactgctttt tgtggaatcg atattcgtca tagcctaacc
     3841 taccgatcca atcatttgaa gctgaattaa gcggttcatc tcgatggcat attccatcgg
     3901 caactctttt accaggggtt caataaagcc cgaaacgatc atggttgtag cttcttcatc
     3961 cgtcagacca cgactgttaa gataaaacag ttgctcttcg ccaatgcggc ttaccgatgc
     4021 ctcatgtccg atgttcacgt cacttgaacg gatatcaatg gttggataag tatccgacac
     4081 agattcaggg tcaagcaata aagcgtcaca aacaaccgaa gagtttgcct tatcaacatt
     4141 attggctacg cgcaccaagc cacggtaaga agatcgccca ttaccccgcg aaatcgactt
     4201 cgacgtaatt ttactgctcg tatttggcac aaagtgaatc atctttgagc ccgcgtcttg
     4261 cacctgccct ggccccgcaa aagcgacgga aagcacctcg ccatgcgcgc cctccccacg
     4321 cagataacaa cttggatact tcatggtcac ctttgagcca aggttggcat cgacccattc
     4381 catcgtgcca ttttcgtaaa ctaccgcgcg ttgggtaacc aggttataca cgttggtaga
     4441 ccagttctga atggtcgaat agcgcacacg ggcattcttt ttaacaacaa tttcgatgac
     4501 accgctatgg agagaatctt ccgaataaat cggcgcggta caaccctcca catagtgtac
     4561 ctgcgagcct tcatcagcaa taatcaaact ccgttcaaac tgccctaagt taggattgtt
     4621 cagccggaaa tatgcctgaa gtggcaaatc aaccttgaca cctttcggga catacacaaa
     4681 cgaaccgccc gaccaaaccg cggtgtttag cgcgctgaat ttattgtcct gatatggcac
     4741 cactgttcca aaatgttctc taaacagctc tgggtactct ttgagaccat tttcaatgct
     4801 caaaaaaaca acaccttgtt tctcaagatt ttcctgaata ctgtgataga ccatctcgga
     4861 gtcatattgt gcgccaacac ccgccaaaaa cttttgttcc gcctcaggga tacccagctt
     4921 atcaaaagtg tttttgatag tatctggcac gtcatcccag gatttttggt ttacttcagg
     4981 tggtcgaaca tagtagtaga tattgtccag gtccaaatca ctgagatctg gtccccagcc
     5041 aggcatggga cggttgatat agtgctcgta ggcttttagg cgaagattta acatccattc
     5101 aggttcttct ttaaaagccg atatctgctt cacaatctcg cgggtcaagc ctttttccgg
     5161 cctaaaactc gcgatatcgg ggtcactaaa gccatattga taatcagccc caagttcgtt
     5221 tagaaagtct tggttttttt gatcactcat tcttatccaa actttcctta tccggttttc
     5281 cgcgtgtcag gatacttgtc gcgaatccag tcatagcctt gttcttcaag tttaagagcc
     5341 aactccgccc cacctgattc aacaatcgcg ccaccataca taatgtggac aaattgcggt
     5401 tgcatatact ccaacatacg ttggtagtgg gtaatgagaa taacgccgag agtatcggat
     5461 tgcaattgat gcacaccatc agcgacaatc cgcagagcgt caatatcaag ccctgagtca
     5521 gtttcatcca aaatagcgat gcgtggctca agcattgcca tttgcaaaac ttcagcacgc
     5581 ttcttttcac cgccagaaaa accatcgtta agatatcgtg cgccaaatgc gtaatccatc
     5641 ttgagcaaat ccattttcga acgcaataaa cgtctgaaat taaccaggct tatgccctta
     5701 tcttctggat tgagtgcctt tgctttggcg tttagtgcag agcgcagaaa atttgccaaa
     5761 gtaacgccag gaatagcaac cggatattga aaagctaaaa acaaacctcg tcgtgcccgt
     5821 tcatcgaccg acatttccat gatgctttca ccatcaagca aaatatcacc tttgataact
     5881 tcgtagcgcg gatgccccat cagagcgtac gccagagtcg attttccggt accattgggg
     5941 cccattagtg cgtggacttc accgccattg acggtaagat tgaacccttt taggatttct
     6001 ttctcaccga tattgacgtg taaatctttt atttcaattt ttgtcataaa acctcattat
     6061 ttatttggtt tttaccacgc cgattatagc aaatcggcag gtggaagtca atttcttgta
     6121 ttatttacta tatttttatg ctattaattc gcgttgccaa tcaacacgat ttgtaaatgt
     6181 ctgcacacga ttagccttta aagccccgtt cttgctttgt ttcacttgaa aaagtaaacc
     6241 tttgtttgat aattatcatt ggacaaggtt ctcaacatgg gtataatatc ggtgtttaac
     6301 aatggctatt taatgctatt accaattctt tccgccagga agaagatatt caatgaggag
     6361 attatcatgt ccgagaaaaa aagtttgaaa gatatcatca aaccaaagaa acaggaaaag
     6421 tcgcttgcga cacccgagcc cgccccaaaa gaaaccctta caaaacgcga agcagttcgc
     6481 caaaagttag aggatttgcc caaagagcct gccaggaccc acgtcgtagc agaaggcgaa
     6541 accctttcgc acatcgctct caaatactat ggcaaagcca cgcccccata ttatcagcac
     6601 atctaccaac acaatctcga agttattggc gataacatca acatcatcgt ccctggtcag
     6661 gaattggtta tccctgactt gccagacgaa ttaaagtaaa aaaagtatca aggaataatt
     6721 atgaccaaag agctcgacct tgctcaagtt tttaaagcgg tatccaaaac actcgtcgaa
     6781 aaccaatcaa gcttagatga agcggatacc gtcaatcacg accacggcac aaacatggtc
     6841 aacaccttca aactcatcga aaaagctgtc aaagccaaaa aagatggtac aaccaccgaa
     6901 caactggcat atgccagcca aatgctgcgc gaaaaaagca ccagcggttc tgcgatgctt
     6961 tacgccgatg gacttgaaaa tgcggcaaaa gaatttgaag ggaaaacatt tgaccgcgaa
     7021 ggtgtcggca ccctgctcaa tgccttgatg ggtacacccc aaaaccaggc acaggaacaa
     7081 acaccatctg caccacaagg aaacgatttc ctttccacac tggttggcgg aatggccagc
     7141 tcacaacaaa cttcccaggg agaacaagag ccaactttcg cccaggttcc agatttgatt
     7201 gacgccttag gtggtctcac cttcacccag gaacctgtcg cgcaagaagc tgaacaaaac
     7261 caggaaagcg accaaacttc agcaattgac cttgttggct cgcttttggg aggactcggt
     7321 caaacacaac ctgcaaaaca ggaagaagaa cccaaaccaa gtggacttgg tggtcttttg
     7381 ggcaacctcc ttggtggagg aaaacaacaa agtccggcat cttctggcgt tggcggatta
     7441 ggaaaccttt taggctcact tttaggtggc caacaaagcc agccttcgca acaagctggg
     7501 caagcagcct ctaccgctac aagcttgctt tcagctgccc tggcctactt ggcagccacg
     7561 caacaaggca aaacaccctt tgaatccatc ctggaagcag ttggtactgc aacgcgcttt
     7621 agcgaccgcc aggaccgcac ccaatcaggc gcgcttgttg ctcagacatt aattggattg
     7681 ctatccaagt agtcacatta acgaccatag acaaaaaacc gcgcgaattc gcgcggtttt
     7741 ttattattat tttttattct aaccctgact gtttttagta gaatacggct cggtccatgc
     7801 ctttagcctg caaaatccaa tcagcaacca tcgactggct tggtggacgg cgaacaagat
     7861 tcttttcatt gttcacttca ataatttttg cgtagagatt ctcagggttg cgtgttgcca
     7921 gttcaacaac agtatccaca ccagcttctt caagcaaatc agaatactct tcgccaacac
     7981 ccttgatacg ataaagatca gcgtgattaa cccattcgag aataagtttt gaactaatac
     8041 ctgtttgttc tgcaatctct tctcgtcctt ttgggtatgc acctgcttta agcaggcctt
     8101 caacggtctt aatgccaata gcgatcagtt tttcggcata gacaggtcca ataccttcga
     8161 tatcaataat tttagccatt tttactcctt gtgacataaa gtgtttacac ttaattttga
     8221 ttatagcact aacctagcag attacgtaat aatttacatt tgtatacttt cccattatgg
     8281 ataattataa attatacatt aataagcaac tttcatttta ttctggtaga attgaaataa
     8341 gaaaaatttt acatggagca ctaaattgaa tacaaaaaat cgcattttga acacattact
     8401 tattaaacaa gaagcctcag ttaacgattt agcagaagaa ttaggcgtga atggcatatc
     8461 cattcgacac catcttaaaa actttgaaaa agatggctgg gtcgttagtc gggaatcacg
     8521 aaaaggccag gttggacgcc caaaatttat ttacagtctc agcagtcttg gcatggaaca
     8581 gtttccttca tcttatgtgg aattttcatc aatattagtc gacgagctta aaaaccgcct
     8641 ggatgatgag caactcaacg cgctattcaa agaagttggt ctgcaattcg ccgaaagaaa
     8701 ccgccttgaa cgccgccatc gcaactcacc caaacgtctg tcttacgtcg ttgaaaattt
     8761 aaacgagcaa ggctacatca tcgaaaagaa agaaattgat ggtgaaatcg tctttactaa
     8821 ctaccattgc ccctaccgct atgttgcctc agcgaccaca aacctttgtt gcctagacaa
     8881 gacaatgctc gaagaactat gcgaaagtga agtagaaatc acgcaaacta ttgccaaagg
     8941 cgacccgcat tgcaccttca tcatcaaaaa caaatgaccc ttagcaacaa acctgactgg
     9001 aagatagaca aaacccaccc ggaacttgtc gaaagtctcg tgttgggttt atctgccgtc
     9061 cttgacccgg acttgggcta ttctgtcctg gaattagggt tggtgcgaga catcagcctg
     9121 accgacaaag gcgcaagcgt ggtgatgata ctcaccacac cattctgccc ctatggtccc
     9181 gctatgctgg aagaaacccg tgccaaggtc gcccaggtgc tcgaaaagcc aaccacaatt
     9241 gaatacggca gcgaagcctg gaaaccagaa atgatggacc cggaacttcg cgatgatgaa
     9301 tggggattgt taggctttta attaccagtt aatttaacgc atcagcaaaa tttcatagtc
     9361 ccttgtcagc aaaagacaat ctcgttgaca agggattttt tctttcaaat agaagaaata
     9421 acacaaaagc atccgtttca cttttttggt gtagcacctt tttagttttc tgcacagtaa
     9481 aatactgtat aataccggac tgaaggtagg atttcctatc tatctaagat tctttatgca
     9541 ataacaaagg agacatatgg acaaatacga atgcatcgca tgtggttaca tctatgaccc
     9601 cgaacttggc gaccccaccc aggatatcgc ccccggaaca ccttttgaag atttgcccga
     9661 agattgggtt tgccccgatt gtggcgttgg caaggatcaa ttcgaaaaga tttaaatcca
     9721 aacaaacctg ttctaaaaaa gactgttgcc atttgtgaca gtcttttctt ttttcccgaa
     9781 aactgtcgta taataaagcc actatattcg ttcaagattg gaggaaaatc ttatggcaag
     9841 tgtgacatac gatggtgtat ggaaaaagtt cggtgacgtg gtcgccgtgc aggatttaaa
     9901 tcttgcaatc gaagataaag agtttttagt tttagttggc ccttccggct gtggcaaaac
     9961 aaccgctttg cgttgtttgg ctggtttgga agaaatcacc gaaggtgaag tttatatcgg
    10021 cgacgttctc gtcaacgata ttgcccccaa agaccgcgac atcgcgatgg tattccaaag
    10081 ctacgcgctc tacccccaca tgaccgtgtt cgacaacatg gcatttggtc ttaaactccg
    10141 caaaacaccc aaagaggtca tcaaccaacg tgtgcaagaa gcagccaaaa cccttggtat
    10201 cgaaaatctc ttgcatcgca aacccaaaga gctctctggt ggtcaacgcc agcgtgttgc
    10261 tgttggtcgc gcaatcgttc gcgagccaaa agtcttcctc tttgatgaac ccctttcaaa
    10321 cctcgatgcc aagttacgcg ttcaaacccg cgcggaactg agcaaactgc acaaacgcct
    10381 tgaaacaacc ttcatctacg tcacccacga ccaaaccgaa gccatgacca tggcaacccg
    10441 catcgccgtg atgaacaaag gcgtcttgca acagcttgac accccacagg ttctgtatga
    10501 ccacccagcc aatctctttg ttgctggttt catcggttca ccttcaatga acttctttga
    10561 cggtgtcatt gaacaacgcg aaggtggcat ttatgtcaac ttaggtagct tcgctgtggc
    10621 tgttccggaa aaagattatg aggccctcaa gcatctcatc ggacgcccaa tcatctttgg
    10681 tatccgcccg gaagatattc acaaccccac ctacgcgcct cctggcatca ttgccgagcc
    10741 ggtaaacgcc gaagtggacg ttatcgagtt gatgggtaac gaaatattcc tgtatctcgt
    10801 caatggtgag cataacttca tcgcccgcgt tgacccacgc acccgcatgg acatcggcga
    10861 caaagtcgaa gtcgctttca atatggataa ctaccatatc tttgatccag gtctcgacga
    10921 agaaaacccc gttgcggttt tctaatccac aagtttaatt gataaagcga gctgacggaa
    10981 tggtcagctc gcttttttat ttgaagttcg accaaatttt atggcgtagc cagtgatttt
    11041 ttctgcttat ttttgcggac ttttgaaatc acaccaaaga taataagcag cagtcctgca
    11101 aaaagcaaag tcagcacact aaccaaaata aatgcgtccc gccaaaatgt tagcgggaaa
    11161 agacgaataa gcgtatcgct ctcgtaaaac aaccaggtgc catctttgaa aaaaagctga
    11221 tggaaacggt caaacaagtt gttaaatgaa acagcgatga aaatgattag cgcgacaatc
    11281 aggcctactg tcaaccaggc tccaccaatc aaaccctgtc tgagtggctt ccacgcgccc
    11341 atgatgacaa accacatcag taccgcgaac gtgaagccta ataaggcata ccaaaccgtc
    11401 aggctcgtct ttacgacctt cttcacatcg atcatgtgtt ccagttcgcg cgcgttaaaa
    11461 ac
//
LOCUS       DAOSFC010000012        10446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_5395_length_10446_cov_6.110673, whole genome shotgun sequence.
ACCESSION   DAOSFC010000012 DAOSFC010000000
VERSION     DAOSFC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5395_length_10446_cov_6.110673"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..487)
                     /gene="rpsK"
                     /locus_tag="PK791_00665"
     CDS             complement(83..487)
                     /gene="rpsK"
                     /locus_tag="PK791_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOH91858.1"
                     /translation="MAKQARTARRATSSRKVKRQLSAGQVHVFASFNNTIVTITDMRG
                     NTVCWGSSGTAGFKGSRKSTPFAARMATEQALKTAQQMGIQEVELFVKGPGPGREAAI
                     RAVQGMGVKVTKITDETPVPHNGCRPPKKRRV"
     gene            complement(595..981)
                     /gene="rpsM"
                     /locus_tag="PK791_00670"
     CDS             complement(595..981)
                     /gene="rpsM"
                     /locus_tag="PK791_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOH91859.1"
                     /translation="MARIEGVDLPRNKRIDIGLRYIYGIGPKTANDILAATGVDPAIR
                     VKDLPESEVTKLRDYIAQNLKVEGDLRREVQMNIKRLVEIGCYRGLRHRRNLPLRGQN
                     TRTNARTRKGPKKTVAGRGRRRGAKK"
     gene            complement(993..1106)
                     /gene="rpmJ"
                     /locus_tag="PK791_00675"
     CDS             complement(993..1106)
                     /gene="rpmJ"
                     /locus_tag="PK791_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOH91860.1"
                     /translation="MKVSASIKKRCKDCKIVKRDGRLYVICKNPRHKQRQG"
     gene            complement(1177..1959)
                     /gene="map"
                     /locus_tag="PK791_00680"
     CDS             complement(1177..1959)
                     /gene="map"
                     /locus_tag="PK791_00680"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOH91861.1"
                     /translation="MDSYEIFGVNLKSANELKIMREAGRLNALTLKAVMDAIAPGVTT
                     GELNEVAESFQKAHGVYSPFKNYDPGNGVPYPGSICTSINEEMVHGIPGNRKLREGDI
                     ISVDCGTVYQGYVGDSAFTVGVGEISKQAQSLIDTTKQALEIAISKMVIGNRSGDVGA
                     AIEAYVESQGFYCTHTYTGHGVGRAMHEAPQLPNYGKPGTGFLLREGMTIAIEPMVLV
                     GGRRTRVLRDRWTVVNANGGLTAHQEHTIAVTKDGPLVLTEL"
     gene            complement(1959..2612)
                     /locus_tag="PK791_00685"
     CDS             complement(1959..2612)
                     /locus_tag="PK791_00685"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOH91862.1"
                     /translation="MKEQATIVVMLGAPGTGKGTQAVLLAERLGIPHISTGDLFREHL
                     AAETPLGIEAATYMNKGELVPDEITITMLNARLEEDDCENGVVLDGYPRTLVQALALE
                     SYLDSIDEELTMVANLAVPEALIVKRLSGRWMSKSGNVYHAEYNPPKRKWFDDEDGSP
                     LYQREDDKAETVLNRLKIYEAQTLPLIDHFREEGKLVEFNGVGEINDINDEIFEALQ"
     gene            complement(2627..3997)
                     /gene="secY"
                     /locus_tag="PK791_00690"
     CDS             complement(2627..3997)
                     /gene="secY"
                     /locus_tag="PK791_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOH91863.1"
                     /translation="MKRSSWRYLWKSESIRNKLLITLLLLAVYRFVANVPVPGLDHAA
                     VKELSQLTGAGGNLVGLLDLLSGGAVSKFSVLAMGVYPYITAQIILQLLTPVIPALEQ
                     KMKDDPREGRVWMEKWTVILTIPMALLSAFGQINIFNQMLGGQSVIQHYGFSGENLLP
                     TLAAIITMVAGTMFGIWLGQLISEFGLRNQGLSLIIFSGIVSRFPVTIAQMVRRADPA
                     NPGQYISDPWWAFVLIFVVFILTIFGIVYMQLGRRNVPVMFPGRRVGNRMSMPVRSQL
                     PLMINMAGMIPIIFAQSFLTFPAILSSFFLNKTNWVGKAANWVYRNFGGDTWWYPIMF
                     FVLVVLFCYFYTDVLFAQQDYGENLKKQGAQIPGVVRGKPTQDYLTRVQRRITLPGAI
                     FLGIIAVLPYIMTGVFKLFGETNIAGLGLMQASGLLIVVGTVREIVQLIETELRMHGY
                     DDRLIN"
     gene            complement(4019..4477)
                     /gene="rplO"
                     /locus_tag="PK791_00695"
     CDS             complement(4019..4477)
                     /gene="rplO"
                     /locus_tag="PK791_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOH91864.1"
                     /translation="MQLHDLKPNEGAKKGRKRVGRGTGSGMGKSAGRGTKGQSARTSG
                     GVRLYHQGGNLPFYRKLPFLRGEGFTPVNRVRYAEVNLDALTPYKKGDEVNPENLKAN
                     GLLKGKGLPVKIMGRGEIKVALNIKAHKVTEGARQKIEAAGGTIELIELA"
     gene            complement(4479..4664)
                     /gene="rpmD"
                     /locus_tag="PK791_00700"
     CDS             complement(4479..4664)
                     /gene="rpmD"
                     /locus_tag="PK791_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOH91865.1"
                     /translation="MLRIKLVKSGIGYTVKTKRTLSALGFKKLQEVVEHEDTPIIRGM
                     LDKVSHLVEIESALEEK"
     gene            complement(4684..5208)
                     /gene="rpsE"
                     /locus_tag="PK791_00705"
     CDS             complement(4684..5208)
                     /gene="rpsE"
                     /locus_tag="PK791_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOH91866.1"
                     /translation="MDEFQGLEQQYDERVIDIARVAKVVKGGRRFSYRVTLVVGDNNG
                     NIGLGIGKALAVPDAMRKASDRAYKNMRPVSLHGTSIAHEVIGVQSGAKVLLKPASPG
                     TGVIAAGGVRAVLEAAGITDILTKSLGSANMLNIVLATFNALDQLKSPEIVALERGKD
                     VRDVTPYWDRRRRG"
     gene            complement(5221..5586)
                     /gene="rplR"
                     /locus_tag="PK791_00710"
     CDS             complement(5221..5586)
                     /gene="rplR"
                     /locus_tag="PK791_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOH91867.1"
                     /translation="MAKKSRNQARLHRAKRVRKKIFGTPERPRLNVFRSINEIYVQII
                     DDHSGTTLASASSVDKSLRDAMEGKNKIDQARLVGEEIAKRALDKGIKKVVMDRAGYF
                     YTGRIKSMAEGARKGGLEF"
     gene            complement(5595..6143)
                     /gene="rplF"
                     /locus_tag="PK791_00715"
     CDS             complement(5595..6143)
                     /gene="rplF"
                     /locus_tag="PK791_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOH91868.1"
                     /translation="MSRIGKLPITLPKGVEVKIDGQKVSVKGPKGQLERTFAPEIELK
                     LEDGTITVSRPNDEPHVRALHGTTRALLNNMIVGCGEGFTRVLEYNGVGYRAELQGKD
                     LVLNVGYSHPVNYPAPDGIEFDVDARARTIKIMGRDRQQVGQVAAEIRKVRPPERYHG
                     TGIRYQGEEVKLKAGKSGRTGK"
     gene            complement(6154..6567)
                     /gene="rpsH"
                     /locus_tag="PK791_00720"
     CDS             complement(6154..6567)
                     /gene="rpsH"
                     /locus_tag="PK791_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOH91869.1"
                     /translation="MTITDPIADMLTRIRNGAMAGLSTVAMPHSKLKAEVARILVEEG
                     YLADYEVLEGKNETHKVLQVKLKYIGERRQRRPLITGLQRVSSPGCRVYTSKKDIPWV
                     LSGIGIAILSTPKGVMTGARARKLGVGGEILCKVW"
     gene            complement(6591..6773)
                     /locus_tag="PK791_00725"
     CDS             complement(6591..6773)
                     /locus_tag="PK791_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOH91870.1"
                     /translation="MAKKSMIHREYRRKYPTRVVSRCRVCGRPRGYMRKFGLCRICFR
                     ELALQGKIPGVKKSSW"
     gene            complement(6773..7318)
                     /gene="rplE"
                     /locus_tag="PK791_00730"
     CDS             complement(6773..7318)
                     /gene="rplE"
                     /locus_tag="PK791_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOH91871.1"
                     /translation="MNRLLEQYKNEYAPALFEELGLDNVMQVPKIKKVVINIGVGEAL
                     ENPKALDAAVNDLTIITGQKPIIIAAKKAISNFKLREGRQIGVKVTLRGEKMWAFLDR
                     LINVALPRVRDFRGISADSFDGRGNYTLGLTEQLIFPEIQYDKIDKVRGMEISIVTNA
                     TNDDHGRALLSKFGMPFMKGN"
     gene            complement(7322..7741)
                     /gene="rplX"
                     /locus_tag="PK791_00735"
     CDS             complement(7322..7741)
                     /gene="rplX"
                     /locus_tag="PK791_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOH91872.1"
                     /translation="MKIRKGDRVRVMRGDEADKNKVGEVIRVLPKENRVVVQGVNLVK
                     KHQKQQQTGGGKTIPSGIREFEAPIHASNVMVVDSITERASRRVRRGKAAEEAKPATK
                     RATAKKETVAAEPKAKATEAKKSATPKTAKADEGAGK"
     gene            complement(7773..8144)
                     /gene="rplN"
                     /locus_tag="PK791_00740"
     CDS             complement(7773..8144)
                     /gene="rplN"
                     /locus_tag="PK791_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOH91873.1"
                     /translation="MIQKESRLKIADNTGAREILVIQVVGGTRRRYATVGDIVTATVK
                     SATPQSSVKKSEIVKAVIVRVSKAYRRADGSTICFDDNAAVILDNDGVNPRGTRIFGP
                     VARELREKGYMKIVSLAPEVY"
     gene            complement(8147..8503)
                     /gene="rpsQ"
                     /locus_tag="PK791_00745"
     CDS             complement(8147..8503)
                     /gene="rpsQ"
                     /locus_tag="PK791_00745"
                     /inference="COORDINATES: protein motif:HMM:NF004123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOH91874.1"
                     /translation="MNNRRRLNGVVKSNKMDKTIIVDVDRTFAHPLYHKVVTRKKSYK
                     AHDPFGCNVGDKVTIVESKPISRTVRWVVEKILVEEIRQENIESVQEMEQAIEEAIVE
                     ETEETVEEAEQAEDEE"
     gene            complement(8503..8709)
                     /gene="rpmC"
                     /locus_tag="PK791_00750"
     CDS             complement(8503..8709)
                     /gene="rpmC"
                     /locus_tag="PK791_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOH91875.1"
                     /translation="MKIKEIRELSTEQIKTNLLNTRQELMNLRFQMVTGQLTDTSRFK
                     AARRDIARFETILNERARAEQEGK"
     gene            complement(8709..9134)
                     /gene="rplP"
                     /locus_tag="PK791_00755"
     CDS             complement(8709..9134)
                     /gene="rplP"
                     /locus_tag="PK791_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOH91876.1"
                     /translation="MLMPKRVKYRKQMRGRMKGKALRGSTVSFGEFGLKALECGYISA
                     RNIESARRAIVHMMKRRGKVWVRVFPDKPYTKRAAETRLGKGKGAVDHWVAVVRPGRI
                     LFEITTADPEVAKAALNRARYKLGFKTKIVAREVLGEGS"
     gene            complement(9162..9839)
                     /gene="rpsC"
                     /locus_tag="PK791_00760"
     CDS             complement(9162..9839)
                     /gene="rpsC"
                     /locus_tag="PK791_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOH91877.1"
                     /translation="MGRKVHPIGFRVGVNKPWLGRWYAEGKEYTEQLHQDLAIRELVR
                     KEAERAGVSKIEIERFPGKVKVTLHTAKPGILIGRKGESVKALRQELEALTGQKIDLE
                     VKEIKDPDTDAYLVALNIAGQLERRISYRRAMKRAIQQAMRQGAEGIKIQVGGRLAGA
                     EMARSITLREGRVPLQTLRADIDFSRASALTTFGQIGIKVWIYKGIVMHQAEEKVEAT
                     EGVYVSE"
     gene            complement(9849..10196)
                     /gene="rplV"
                     /locus_tag="PK791_00765"
     CDS             complement(9849..10196)
                     /gene="rplV"
                     /locus_tag="PK791_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOH91878.1"
                     /translation="MAQDIIAKLSNFANSAQKTRLVIDLVRGKPALKALEILATTPNK
                     AAMPVRKLIYSAISNAEENFGISRDDLVVYRIFADEAPTRKWRRFGARGRFKPMLKRR
                     SHITVILRETEQN"
     gene            complement(10202..10444)
                     /gene="rpsS"
                     /locus_tag="PK791_00770"
     CDS             complement(10202..10444)
                     /gene="rpsS"
                     /locus_tag="PK791_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOH91879.1"
                     /translation="MDPKLLKKVEDMNAKGDKKVIRTWSRASVIFPQMVGHTIAVHDG
                     RRHVPIYVTENMVGHRLGEFAPTRWFRGHVAEKKGR"
BASE COUNT         2449 a         2742 c         2387 g         2868 t
ORIGIN      
        1 ggaacagctt ttcgccttcg cgtcgacata atcgacaaac tgcttctgta tatctagcca
       61 taagtttttc ctttttctaa tattaaacgc ggcgcttttt aggcggacga catccgttgt
      121 gtgggacggg ggtttcgtca gtaatcttgg tgaccttaac acccatgccc tgcacggcac
      181 gaattgctgc ttcgcgacca gggccggggc ccttcacgaa caattcaact tcttgaatgc
      241 ccatttgctg ggcggttttc agcgcttgct cggtagccat tcgggcggca aaaggtgtgc
      301 tcttgcggga acccttaaag cctgcggtgc ctgagcttcc ccaacaaacg gtgttaccgc
      361 gcatatcggt gatggtaaca atcgtgttgt tgaaggatgc gaatacatgc acctgtccgg
      421 cagacaactg gcgcttgact ttgcgcgaac tggttgcgcg tcgcgcggtt cttgcttgtt
      481 tagccataaa tcatcctcac tatcatctaa ctctggtacc catcgtcggt tgccgctagg
      541 gagaaaggga accccaccga ctttggatca gaccagttaa taagatatca ataattactt
      601 ctttgctcca cggcggcggc cacgacctgc aacagtcttc ttggggcctt tgcgtgtgcg
      661 cgcgtttgtg cgtgtgtttt gtccacgcag ggggaggttg cgtcgatggc gcaagccacg
      721 ataacaaccg atttcgacca agcgcttaat gttcatttga acttcgcggc gcaggtcacc
      781 ctcaacctta aggttttggg cgatgtagtc gcgtaatttt gtaacttcag attctggcaa
      841 atccttcaca cgaatcgcag gatccacacc ggtggcagcc agaatatcat ttgccgtttt
      901 agggccgata ccgtagatat atcgcaatcc gatatcaatg cgcttgttgc gcggtaggtc
      961 aacaccttca atacgagcca taattctttt ccttatcctt gtcgctgttt gtgtcttggg
     1021 ttcttgcaaa taacgtacaa acgaccgtcg cgtttgacaa tcttacaatc tttacagcgt
     1081 ttttttattg aagccgatac tttcatttgg catactcctt atttttaccg caaagtttca
     1141 attatagtct tttaccgcgg ttatggcaag actttcctat agttccgtaa gcaccaatgg
     1201 gccgtctttg gtgacagcaa ttgtgtgctc ctgatgcgcg gtaagcccac cgttggcatt
     1261 gactaccgtc caacggtcac gcaaaacgcg cgttcttctc ccaccgacca ataccatggg
     1321 ttcaatggcg atggtcatac cttcgcgcaa cagaaaaccg gtgcccggtt taccgtagtt
     1381 tggcaactgt ggcgcttcgt gcatggcacg cccaacccca tgtccggtat aggtatgggt
     1441 gcaataaaaa ccttgacttt caacgtacgc ttcaatggcg gcgccaacgt cgccactgcg
     1501 gtttccgatg accattttgc taatcgcaat ttcaagagcc tgtttggtgg tatcaatcaa
     1561 gctttgagct tgtttgctaa tctcaccaac accgaccgta aaagccgagt cgcccacata
     1621 accctggtaa actgttccgc aatccaccga aataatatcg ccttcgcgca gttttcggtt
     1681 tccgggaatg ccatgcacca tttcttcgtt gatgctggtg cagatgctgc cggggtaagg
     1741 cacgccattg cctgggtcgt agtttttgaa cggtgagtat acgccatgcg ctttctggaa
     1801 gctttcggca acttcgttta gttcgcctgt ggtcacgcca ggagcgatgg catccatcac
     1861 ggctttcagg gtgagcgcat ttaagcgtcc agcctcgcgc atgattttca gctcgttggc
     1921 tgatttgaga ttaacaccga agatttcgta gctatccatt attgcaaagc ctcaaatatt
     1981 tcgtcgttaa tatcatttat ttcaccgacg ccattaaact cgaccaactt gccctcttcc
     2041 cgaaaatgat cgatcagcgg aagcgtttgg gcttcataaa ttttcaagcg attcagcacg
     2101 gtttccgcct tatcgtcttc acgttgataa agcggactgc cgtcttcgtc atcaaaccat
     2161 tttcttttag gcggattgta ttccgcatga tacacattgc cacttttgct catccagcgt
     2221 ccgctaagtc gctttacaat caacgcttcc ggaacagcca gattcgcgac catggtcagc
     2281 tcttcgtcga tactgtccaa gtaggactca agcgccaaag cctgcacaag tgtacgcggg
     2341 taaccatcca aaaccacacc attttcgcag tcatcttctt caagacgcgc gttcaacata
     2401 gtaattgtga tttcatcagg taccaattcg cctttgttca tataagtagc agcctcaatc
     2461 cccaggggtg tttccgcagc aagatgctcg cggaaaaggt ccccggttga gatatgcgga
     2521 atccccaggc gttcagcaag cagaacagct tgtgttccct tgcccgttcc gggggctcct
     2581 aacatcacaa cgattgtggc ttgctctttc atcgattaaa gccctcttag ttaatcagcc
     2641 tgtcatcgta gccgtgcatg cgcaattctg tttcaatcag ttgcacaatt tcgcgcacgg
     2701 tacccacaac aatcaaaaga cccgaagcct gcatcaagcc caaacctgcg atgttcgtct
     2761 cgccaaagag cttgaaaacg ccagtcatga tgtagggcaa aacagcgatg atacccagga
     2821 agattgcgcc aggcagggta atgcggcgtt gcactctggt gagatagtct tgagtgggtt
     2881 tgccacgcac aacaccagga atctgagcac cttgtttctt cagattttcg ccgtagtctt
     2941 gttgcgcaaa gagcacgtct gtatagaagt aacagaacaa aaccaccagg acaaagaaca
     3001 taataggata ccaccaggta tcaccgccaa agttcctgta aacccagttg gcagcttttc
     3061 caacccagtt tgtcttgttc aggaagaacg atgacaaaat cgcggggaag gtcaaaaagc
     3121 tttgcgcgaa gataatcgga atcatacctg ccatgttaat catcaggggc aattgactgc
     3181 gcacgggcat cgacatgcgg ttacccaccc ggcgacccgg gaacataacc ggcacgttgc
     3241 gtctgcccag ttgcatgtag acaatgccaa agatggtcaa tataaagacc acaaagatga
     3301 ggacaaacgc ccaccatggg tcagatatat actggccagg gttagccgga tcagctcggc
     3361 gcaccatttg tgcgatagtc actgggaagc gcgacacaat acccgagaag atgattaggc
     3421 taaggccttg gttacgcagg ccaaactcag aaatgagctg tcccagccaa ataccaaaca
     3481 tcgttccggc gaccatcgta ataatcgccg ccagggtagg caagaggttt tcaccactaa
     3541 aaccatagtg ttggataacc gactggccac ccaacatttg gttgaagata ttaatctgcc
     3601 caaaagctga aagcaatgcc atcggaatgg taagaataac agtccatttt tccatccaga
     3661 cacggccttc gcgtgggtca tctttcatct tttgctcaag cgccggaata actggggtta
     3721 gcagttgcaa aataatctgc gcggtaatat acgggtaaac acccattgcc aacacagaaa
     3781 atttcgaaac cgcgccaccg gaaagcaggt cgagcaagcc aaccaggttg ccaccggcac
     3841 cggtaagctg gctcagctcc tttacagcag cgtgatctaa cccaggcacc ggcacattgg
     3901 caacaaaacg atatactgcc aacaataaaa gcgtgatgag cagtttgttg cgtatacttt
     3961 cagatttcca taaatagcgc caggacgatc gtttcatgtt attgtctcct tatcatcgtt
     4021 acgcgagttc gatcagttcg atcgtaccac cagcagcttc aatcttctgg cgggcaccct
     4081 cggtaacctt atgagctttg atattgagtg caacttttat ttcgccacga cccattatct
     4141 tgacaggcag tccttttcct ttaagcaaac cgtttgcttt gaggttttcc gggttaactt
     4201 catcaccctt tttatatggg gtgagagcgt ccaggtttac ctcggcatag cgcacgcgat
     4261 tgacaggcgt aaagccttca ccacgcaaga agggcagttt gcggtagaag ggcaagttac
     4321 caccctggtg gtaaagtctt acaccaccgc tggtgcgtgc actttgtcct ttggtaccac
     4381 ggccggcact tttgcccatg ccagatccag taccgcgccc aacacgtttg cgaccttttt
     4441 tagcgccttc gttaggttta agatcatgta attgcatctt atttctcctc taacgctgat
     4501 tcaatctcga ccaaatgtga gactttatcc agcatacccc gaatgatggg cgtgtcttcg
     4561 tgttcgacaa cttcctggag tttcttgaag ccaagcgcac tcagggttct ttttgttttt
     4621 acggtgtaac cgataccact ctttacgagt ttaatgcgaa gcatattctt cttagctggt
     4681 tttttagcca cgtctcctcc gatcccaata aggtgtcacg tcgcgcacat ctttgccacg
     4741 ttccagtgcc acaatctcgg gcgatttgag ttggtcaagc gcgttaaacg ttgcgagcac
     4801 aatgttgagc atgttcgcgc tgccaagaga tttggtcaaa atatccgtaa tgccagctgc
     4861 ttccaaaacc gcgcgcacac cacccgcggc gataacgcca gtaccgggcg aagcgggttt
     4921 tagcaacact ttggcaccac tttgaacacc gataacttcg tgcgcgatgg atgttccatg
     4981 caagctgacc ggacgcatgt ttttatacgc gcgatcagaa gctttgcgca tcgcatcagg
     5041 cacagccaaa gctttgccga tgcctaagcc gatgttaccg ttgttgtcgc caaccactaa
     5101 ggtgacacga tatgaaaagc ggcgaccgcc tttgacaact ttcgcaacgc gggcgatatc
     5161 gatgacacgt tcgtcgtatt gttgttctaa tccttgaaat tcatccatag tatcctcgat
     5221 ttagaattct aagccgccct tacgagcacc ttcggccatc gacttgatac gaccggtata
     5281 aaaataacca gcgcggtcca tgaccacttt tttaataccc ttatcaaggg cacgtttggc
     5341 aatttcttcg ccaaccaggc gggcctggtc gattttgttt ttgccttcca tggcgtcgcg
     5401 cagacttttg tccacactcg atgccgaagc cagcgtagtc ccactgtgat catcaatgat
     5461 ctgaacgtag atttcattaa tacttctaaa aacgttcaag cgtggtcgct ccggcgtgcc
     5521 aaatatcttc ttgcgtacgc gtttggcgcg atgcaatcta gcttggtttc ttgatttctt
     5581 tgccatgttt acctctactt accggtcctg ccagacttac cagccttgag tttaacttct
     5641 tcgccctgat agcgaatacc ggtaccatga tagcgctctg gtgggcgcac tttgcgtatt
     5701 tcagcagcga cctggccaac ttgctggcgg tctctgccca taatcttaat ggtgcgtgct
     5761 cttgcgtcta catcaaattc gatgccatcg ggcgcagggt agttaacagg atgcgaataa
     5821 cccacgttca aaaccaggtc tttgccctgc aactcagcac gataacccac accgttatat
     5881 tccaaaacgc gggtaaaacc ttcgccacag ccaacaatca tgttgttcag cagcgcgcgg
     5941 gtggtgccgt gtaaagcacg cacatgcggc tcgtcgtttg gacgactaac ggtgatggta
     6001 ccatcttcta atttcaattc gatttccggc gcaaaagttc gttcaagttg tcctttaggg
     6061 cctttgacac tcactttttg tccgtcaatt tttacttcaa cacccttggg cagggtgatt
     6121 ggtaatttac cgatacgcga catatcctac ctcctaccaa accttgcaga gtatctcgcc
     6181 accgacaccc aatttgcggg cacgcgcgcc ggtcataaca cctttagggg tagacaaaat
     6241 agcgatgccg ataccagaaa gcacccaggg gatatctttc ttcgaagtat acacgcggca
     6301 tccagggctt gaaacgcgtt gcaaaccagt aataagtgga cgacgttgcc gtcgctcgcc
     6361 aatgtacttt agtttaacct gcaagacctt atgggtctcg tttttacctt ccaagacttc
     6421 gtaatcagcc aggtagcctt cctcaacgag gatgcgggca acttcagctt ttagcttgga
     6481 atgtggcata gccacggtgg acaaaccagc catcgcgcca ttgcgaattc tggtcaacat
     6541 atcggcaatt ggatctgtta tagtcataat tctctagcct ccagcttcaa ttaccaggat
     6601 gatttcttta ctccagggat ttttccctga agtgccaatt ctcgaaaaca aatgcggcac
     6661 aagccaaatt tgcgcatata accgcgtggc ctgccacaca ctcgacagcg actgacaacc
     6721 ctggtgggat attttcgccg atattcacgg tgaatcatac tctttttagc cattagtttc
     6781 ctttcataaa gggcatgcca aatttgctaa gcaatgcccg accgtgatca tcattggttg
     6841 cattggtgac gatactgatt tccataccgc gcactttatc aatcttatcg tactgaattt
     6901 cagggaaaat cagttgttca gtaagaccta aagtatagtt gccgcgaccg tcaaaggagt
     6961 cagccgagat accccgaaag tcacgtacac ggggcaaagc aacattgatc aagcggtcca
     7021 aaaatgccca catcttctca ccgcgcaagg taacttttac gccaatttgg cggccttcgc
     7081 gcagtttgaa gtttgaaata gcctttttgg cagcgatgat gatcggtttc tgaccggtaa
     7141 tgatggtcag gtcgttcacc gccgcgtcga gggctttggg gttttcaagg gcttcgccaa
     7201 cgccaatgtt gatgacaact tttttgattt tcggcacctg catgacgtta tcaagaccga
     7261 gttcttcaaa aagggcgggc gcgtattcat ttttgtattg ttcaagtagt ctgttcattt
     7321 attatttacc tgcgccttca tctgccttgg cagtttttgg agtggcggac tttttagctt
     7381 cagtagcttt cgcttttggc tcagctgcca cagtttcttt cttagcagtg gcgcgcttgg
     7441 tagccggttt ggcttcttca gcggctttgc cacgtcttac acggcggctg gcgcgttctg
     7501 tgatgctatc cacaaccatg acattagatg catggatggg cgcttcgaac tcacgaatgc
     7561 cactggggat ggtcttgcca ccgccggttt gctgttgttt ttgatgtttt ttgaccaggt
     7621 tcacgccctg aaccacaacg cggttctctt tgggtaacac gcggataact tcgccgactt
     7681 tgtttttgtc agcttcgtcg ccacgcataa cgcgaacacg atcaccttta cgtatcttca
     7741 tcttcattag acgctccatt cactcattag ctctagtaca cttccggtgc taacgaaact
     7801 attttcatgt agcctttttc gcgcagttcg cgagctactg ggccaaagat acgtgtacca
     7861 cgggggttta cgccatcgtt gtccaaaata acggctgcat tgtcgtcaaa acaaatggtt
     7921 gagccatcag ctcggcgata cgcctttgaa acacgcacaa taacggcttt cacaatttcg
     7981 ctctttttaa cggagctttg tggggttgcg ctttttacgg tagctgtgac aatatcgcca
     8041 acagtagcgt aacgacgtcg ggtgccaccc acaacctgaa taaccagaat ttcgcgcgcg
     8101 ccagtgttat ctgcaatttt taagcgggac tctttttgga tcattgttat tcctcatctt
     8161 ccgcttgttc agcttcttca acagtctctt cggtttcttc aacaatagcc tcttcaatgg
     8221 cttgttccat ttcctggact gattcaatgt tttcctggcg gatttcttct accagaatct
     8281 tctcaacaac ccagcgcacg gtgcgcgaaa tgggttttga ttcgacaata gttaccttat
     8341 cgccaacatt gcaaccgaaa ggatcatgtg ctttgtaaga cttttttcgg gtcacaactt
     8401 tgtgataaag tggatgcgca aaggttcggt ctacgtcaac gatgatggtt ttgtccatct
     8461 tattgctttt aaccacacca tttagtcttc ggcgattatt cattacttac cttcctgttc
     8521 tgcgcgagcg cgttcgttga gaatggtctc aaatcgcgcg atatcacgac gggcagcttt
     8581 aaaacggctt gtatcggtca gttgaccagt aaccatttgg aagcgcagat tcatcagttc
     8641 ctgtcgtgta tttagcaaat ttgttttaat ctgctcagtt gaaagttcgc gaatttcttt
     8701 aattttcatt atgaaccttc tcctaatact tctctggcaa caatcttggt tttaaatcca
     8761 agtttgtatc gggcgcggtt aagggcagct tttgccactt cggggtcagc ggtggtgatt
     8821 tcaaacagga ttcttcctgg gcgcacaacg gcaacccagt gatccacagc acctttacct
     8881 ttacccaagc gggtttcagc agcacgtttg gtatagggtt tgtctgggaa gacacgcacc
     8941 cacaccttac cgcggcgttt catcatgtgc acaatggcac gtcgggcact ctcgatattg
     9001 cgggctgaga tatagccgca ttcgagcgcc tttagtccaa attcaccaaa gctgactgtc
     9061 gagccgcgca gggctttgcc cttcatgcga ccgcgcatct gcttgcggta ctttactctt
     9121 tttggcatca acataatcta atacctcact cgttccttta attactcgct tacgtagaca
     9181 ccttcggtgg cttcgacttt ttcttcagcc tgatgcataa cgatgccctt gtagatccat
     9241 accttgatgc caatttgacc gaaagtggtt aaagcactcg cgcgtgaaaa atcaatatcc
     9301 gcgcgcaatg tttgcaacgg cacacgacct tcgcgcaggg ttatactgcg agccatttca
     9361 gcaccggcca aacgaccacc cacttgaatc ttgatacctt ccgcgccctg gcgcatggct
     9421 tgttggatgg cgcgtttcat agcacggcga tagctgatac gacgttcaag ctggccggca
     9481 atattgagtg ctaccaaata agcgtcggtg tcaggatctt taatctcttt gacttcaagg
     9541 tcaattttct gaccggtcaa cgcttcaagt tcctggcgca gcgctttaac gctttcacct
     9601 ttgcggccaa tcaaaatgcc gggcttggca gtgtgcaggg tgaccttcac tttgccaggg
     9661 aagcgttcga tttcgatctt gctcacgcca gcacgttcgg cttctttgcg cacgagttcg
     9721 cggattgcca aatcttgatg cagttgctca gtatattctt tgccttcagc gtaccaacga
     9781 cccaaccagg gtttgttgac gcctactcga aaacctattg gatgtacttt tcgacccata
     9841 atatctcctt aattctgctc agtctcacgc aagataacag tgatgtgcga acggcgtttt
     9901 aacataggct taaaacgtcc gcgggcacca aaacgtcgcc atttgcgggt tggtgcttca
     9961 tcagcaaaaa tgcgataaac taccaaatcg tctcggctaa taccaaagtt ctcttccgcg
    10021 ttcgaaatcg ctgaatagat gagtttacga acaggcattg cagctttgtt cggggttgtt
    10081 gcgagaattt ccaaggcctt caaagcaggc ttgccacgca ccaggtcaat aaccaggcgg
    10141 gtcttttgtg cagaattcgc aaagtttgaa agcttagcaa taatatcttg tgccattttc
    10201 tttatctgcc tttcttctca gccacgtgcc cacggaacca acgggttgga gcaaactcgc
    10261 caagtcgatg gccgaccatg ttctcggtca catagatggg cacatgtcgg cgtccgtcat
    10321 ggacggcgat ggtgtggcct accatctgcg ggaagataac actggcacga ctccaggtgc
    10381 gaataacttt cttatcgccc ttggcgttca tatcttcgac ttttttcaat agtttcgggt
    10441 caataa
//
LOCUS       DAOSFC010000013         9956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_5781_length_9956_cov_4.614988, whole genome shotgun sequence.
ACCESSION   DAOSFC010000013 DAOSFC010000000
VERSION     DAOSFC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5781_length_9956_cov_4.614988"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK791_00775"
     CDS             <1..332
                     /locus_tag="PK791_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91880.1"
                     /translation="PAQPVQPVQPAPQYYQPPQQAPNDPNRWTGELYTPEVTPPSQPY
                     PHQAPGAWTSNQHVGYAPPPAPEQKDKFPVWAIVLIVLLVLLICIACTVLPFVIFGSM
                     IANFNGF"
     gene            479..1105
                     /gene="pth"
                     /locus_tag="PK791_00780"
     CDS             479..1105
                     /gene="pth"
                     /locus_tag="PK791_00780"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOH91881.1"
                     /translation="MQTAFIEGYTMENDRQDPFLIVGLGNPGIKYRETRHNFGFMVLD
                     ALADELNIPLKKLKYKAMIGEGRLNDAKLILARPLTYMNNSGDAVWQLAKFFKVPHEH
                     IMVAHDDLDLPLGVLRLRPGGGASGQRGVASIIQKLGTQDFPRVRLGISRPPGQMDPV
                     DYVLEKFLPSEKTLRDIVIKEAVAAIQVFVREGLNAAMNRYNGDVSGT"
     gene            1089..>4195
                     /gene="mfd"
                     /locus_tag="PK791_00785"
     CDS             1089..>4195
                     /gene="mfd"
                     /locus_tag="PK791_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOH91882.1"
                     /translation="MSAERKILDIIAELPAYQALLDSLRAKEYKSSERRGLGLPRTVR
                     LALLARLQLDLQVPILFLTNRADRALALYDEIRFWLANGRNYYFPEPNPLFYENLAWS
                     ERTRLDRLHVFSELASAQLPGTNSVKPNIDTIIAPIRALMTRTIPRRDFLKATQTLKI
                     GDERDLVETLSKLVGIGYEYNNIVVQPGQFARRGGLIDLWSPASALPIRFEFFGNEID
                     TIKTFDPATQRSLDSMEMARIFPASEALPEKLATPDKTSLDSHENAHIISEATIPHLY
                     RFHSSLLDYLPENALILLDGEEFISATALDIEEESLKRRDDLIAEKTLDESAPVPYFT
                     WSELTDAFNKLQVLELGHSNAFGGSPLSARFDAGPRFAGRLKDFQNHLKTLEKTRQHW
                     TVVSRQASRLKQLWSETLDPKAPQDSLNNFVEDSLSDGWVLKMSEKQSHHLLTDSEIF
                     GWSRPQPRRRPMLFAEAPEALYDDFKPGDYIVHVDYGIGQFIGLHRPKVDGVQREYLA
                     IGYKDNDTLYVPIQQADRLTRYIGADSAKPTLQRLGGSDWMLTKSRVRGAVAEVATEL
                     LDLYARREAVAGHAFSPDTDWQKELEASFPYIETPDQLDAIAQVKADMESDRPMDRLL
                     CGDVGYGKTEVALRAAFKAVADGKQVAVLVPTTVLAQQHYDTFRERLSVFPVNVEMLS
                     RFRTPKEQDEIIQKLADKKLDIVIGTHRLLSADVQFADLGLVIIDEEQRFGVTHKEHL
                     KKLRSEVDVLTLTATPIPRTLYMALSGVRDISTINSPPEERMPVLTHVGPYDPKLVRE
                     AIIRELDRGGQVFFVHNRVQSIHVIYNQLSALVPEARIAVGHGQMPEKQLAQVMEDFT
                     HGEIDVLLSTTIIESGLDIPNANTLIIDRADTFGLAQLYQLRGRVGRGAQRAYAYLFR
                     DRRRAPTPEGEERLEILAENARLGAGYSIAMRDLEMRGAGDLLGTRQSGYISAVGFQL
                     YTRLLGQAVRRLRQTADPKSLPKLPALALSELPSLDINVELPLGAEIPPDYIPDQALR
                     LTLYRR"
     assembly_gap    4196..4295
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4296..4696
                     /locus_tag="PK791_00790"
     CDS             <4296..4696
                     /locus_tag="PK791_00790"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91883.1"
                     /translation="IPPDYIPDQALRLTLYRRISALRDEKSVDETATEFADRFGALPD
                     GIKDLLYQMRVKILGEARGLDSIGIAGQQIVLSYPPLPQGLKDRGLPELPPPARAGRN
                     AYWIKPNSLAREDWQDLLLDVLRQIPLMAR"
     gene            4752..6188
                     /locus_tag="PK791_00795"
     CDS             4752..6188
                     /locus_tag="PK791_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HOH91884.1"
                     /translation="MMDESVETKENPPDDKKRIKPFWLWALSVCLIAVGIISLFFAFY
                     REYMLVVDDIPYTLKSIALRGRIVLKNAGLEIDDADRVSIDINTYSWELPGEIKIDRA
                     RKVTILHQSETHQLNSAARHPSTLLQLAGIKLYPNDIILQNKQPIDPYAPIPLGQEII
                     IEYIPAKLVLLNDNGQMRSFSSQAETLEQAFAEQNIQINEHDRLSLPLSTKLSLETQV
                     EIRRAREITVMRGENAFVGYSAADNAEEALLEIGLPVQNLDFIAPSGVLAFEENNPIL
                     SLDIVQVSESFNFIKEETAYSNTYELDPNTELDTTTVLVPGQTGYVVTRTISISENGE
                     TVKTYPSQQWKASDPVNGIIGRGTMPVVKTETVDGITLEYWRKVTVYATSYHPSEFPP
                     GARTRSGTPVTKGIIAVSAAWYPLMAGQQVYVPGYGHGVIGDSGGGIPGRYWIDLAYD
                     DENYVGWHHWTTLYFLTPVPAYIPAVLP"
     gene            6185..7051
                     /gene="rsmA"
                     /locus_tag="PK791_00800"
     CDS             6185..7051
                     /gene="rsmA"
                     /locus_tag="PK791_00800"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOH91885.1"
                     /translation="MKFLDTHALIRKYDIRPKKSLGQNFLVEPSGLRKVLQAAELKGD
                     EQVLEIGAGLGSLTVLLAQNAREVIAIEIDQTLFPALREAISKYKNVKTTQGDILELD
                     LKNLLSDEPFVVVANIPYYITSAIIRKLLEAQKRPSHMILTIQKEVAERIIARDGKMS
                     LLALSVQVYGKAELVASIPAGAFYPKPDVDSAVLKLSLYDKPIIDEEKLSVFFELATA
                     GFSQRRKTLRNTISAGMAISNQQAEEMLTSAGIDPKRRAETLSLEEWKELVSVYCDTP
                     SIATSIKQKQKN"
     gene            complement(7048..7770)
                     /gene="rsmG"
                     /locus_tag="PK791_00805"
     CDS             complement(7048..7770)
                     /gene="rsmG"
                     /locus_tag="PK791_00805"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOH91886.1"
                     /translation="MTSRWYEYAEALYGIALNDAQLAQFADYETMLTDWNARFNLTAI
                     RDLDGIRIKHFLDSLSCVLGFPEELDTPAMIDIGTGAGFPGVPLKIAYPNSFLTLVES
                     IKKKANFCEELTRVLGLKRVLVSSERAETIGQDWEHREFYDIALARAVAGMPTLVEYL
                     LPLVKVGGRTILQKGDSAMEEADAARNAIRLFGGELLEIIPVELPEVDGERYLVVIEK
                     VKPTPPEFPRAVGVPSKSPVRN"
     gene            complement(7774..8406)
                     /gene="udk"
                     /locus_tag="PK791_00810"
     CDS             complement(7774..8406)
                     /gene="udk"
                     /locus_tag="PK791_00810"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOH91887.1"
                     /translation="MQAKKKSFVIGVAGASGSGKTTLCKEIVREIGEDKIAVLPHDAY
                     YRDQSSKSFEERVKVNYDHPDSLETELLIQHIHHLRAGTAIDLPVYDYKLHTRAPETV
                     RIEPKPIILVEGILIFAEPSLRKLFDMKLFVDTEQELCFIRRLQRDVVERGRTVDSII
                     EQYLTTVRVSYLEFVEPSKRFADVIIPEGGMNTVAAEMVIARLKALARDV"
     gene            8546..9424
                     /gene="folD"
                     /locus_tag="PK791_00815"
     CDS             8546..9424
                     /gene="folD"
                     /locus_tag="PK791_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOH91888.1"
                     /translation="MTSNIIDGKKLSQSIREDIKKQIETRIAQGKSQPGLATILVGDD
                     PASRTYVRNKLRACDAVGIRNISYELPQDTSQEELDALIKQLNNNPDVTGILLQLPLP
                     KHLDEESLLAAIKLIKDVDGFNPINVGRIALRGIAPVFVPCTPAGILELLREYEAPIA
                     GANAVVVGRSNIVGMPLALLLTKENATVTICHSKTKNLPEITRQADILIVAIGKPEFI
                     TADMVKPGAYVIDVGINSVDDSSRKSGYRLVGDVAFDEVAQVAKAITPVPGGVGPMTI
                     AMLLKNTMRALELAEQ"
     gene            9485..>9956
                     /locus_tag="PK791_00820"
     CDS             9485..>9956
                     /locus_tag="PK791_00820"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOH91889.1"
                     /translation="MQKKGVAVVAVGGNALIKDKDHETIQDQFVAASDTTAHIADMIL
                     AGWDVVITHGNGPQVGFNLRRSELAKDEVFEVPLDYCGADTQGSIGYMFAQALHNEFR
                     RRGIDKKAVAVVTQTIVDKNDPAFENPTKPIGSFMDEATAKERAAEHGWTVVEDA"
BASE COUNT         2561 a         2885 c         2193 g         2217 t
ORIGIN      
        1 cacctgctca accagtacaa cctgtacagc cagctcccca atactaccaa ccaccacaac
       61 aggcacccaa tgaccctaac cgctggacag gggaactcta cacgcctgaa gtcacaccac
      121 ccagccaacc ttacccacac caagcgcctg gcgcctggac cagcaaccaa cacgttggct
      181 atgccccacc tcctgcacct gagcaaaaag acaaattccc ggtttgggcg attgtactca
      241 tcgtcttgct tgttctgctt atctgcatcg cctgcaccgt tttgcccttc gtgatatttg
      301 gaagcatgat tgccaatttc aatggcttct aagctatacc cttagcccat cacaaagact
      361 gccataaggc agtctttgtt ttttagctat tttattaaaa agtatcccat cgcaaacttt
      421 ttatcaggca aattgcattc gcatttcata cgaaaagctg ttaaacttga agattgcaat
      481 gcaaacagca tttattgaag gctatacgat ggaaaatgac agacaagacc cattcttaat
      541 cgtcggatta ggcaatcccg gcataaaata ccgtgaaacc cggcataatt ttggattcat
      601 ggtgttggat gcgcttgccg atgagcttaa tatccccctc aaaaagctta aatataaagc
      661 gatgattggc gaagggcgtc tgaatgacgc caaactcatt ttagcccgtc cgctgactta
      721 catgaacaac tccggcgatg cggtctggca actggcgaaa ttcttcaagg tgccccacga
      781 acacattatg gtcgcccacg atgaccttga cctgcccctg ggagtcttgc gcttgcgccc
      841 tggtggtggc gcttctggac agcgcggtgt ggcatctatc atacaaaaac tgggtacaca
      901 ggactttccc cgtgttcgac tgggcatatc ccgtccgccc gggcagatgg accccgtcga
      961 ttatgtgctg gaaaagttcc tgcccagcga aaagaccctg cgcgatattg tcatcaaaga
     1021 agccgtcgcg gcaattcaag tcttcgtccg cgaaggtctg aacgcagcga tgaacagata
     1081 caacggtgat gtcagcggaa cgtaaaatcc tggatatcat tgccgaattg ccggcatatc
     1141 aggccctgct cgactccttg cgcgccaaag agtacaagag cagcgaacgc cggggcctgg
     1201 gcttgccacg caccgtgcgc ctggcactcc tggcacgcct gcagctagac ctgcaagtgc
     1261 ccatcctgtt tctcaccaac cgcgccgacc gtgccctggc actctacgac gaaatccgct
     1321 tttggctggc aaacgggcgt aactattatt ttccggaacc caaccccctt ttctatgaaa
     1381 atttagcctg gtccgaacgc acccgactgg acaggctaca cgtcttctca gaacttgcca
     1441 gcgcgcaact gcccggcaca aactcagtaa agcctaatat cgacaccatt atcgcgccca
     1501 ttcgcgcctt gatgacccgc accattccac gtcgcgactt tctgaaagcg acccagacgc
     1561 tcaaaatcgg cgacgaacgc gacctggttg aaacactcag taaactggtc ggcataggtt
     1621 atgaatacaa caatattgtc gttcaacccg ggcaattcgc gcgacgcggt ggcttgattg
     1681 acctttggtc gccggcatcc gcactaccta ttcgctttga attcttcggc aacgagattg
     1741 acaccatcaa aacctttgac cctgcaaccc aacgcagctt ggattcgatg gaaatggccc
     1801 gcattttccc cgcctccgaa gcccttcctg aaaaactcgc cacacccgat aaaacctcac
     1861 tcgactcaca tgaaaatgcc catatcatct ctgaggcgac tattccccat ttataccgtt
     1921 ttcattccag cttgctggac tatttgcccg aaaacgcgct tattcttttg gatggggaag
     1981 aatttatctc tgccaccgct ttggatattg aagaagaatc tcttaaacgt cgcgacgatc
     2041 tcatcgctga gaaaacgctt gatgaatccg ccccggtgcc ctacttcacc tggtctgaac
     2101 taaccgacgc attcaataaa ctgcaagtgc tcgagttagg acattccaac gcctttggtg
     2161 gcagcccctt aagcgcgcgt tttgacgctg ggccgcgttt cgccggccgt cttaaagact
     2221 tccaaaacca cctgaaaacg ctcgaaaaaa caaggcaaca ctggacggtc gtctcgcgtc
     2281 aggcttcacg tcttaagcaa ctttggtccg aaacgctcga ccccaaagcc ccgcaagaca
     2341 gcctgaataa cttcgtcgaa gattcgcttt cagatggctg ggtgctcaag atgagcgaaa
     2401 agcaatccca tcacctgctt accgacagtg agattttcgg ctggagtcgg ccacaaccac
     2461 gtcgccgacc gatgcttttt gccgaagcac ccgaggcgct ctacgacgac tttaaacccg
     2521 gcgattatat cgtgcatgtc gattatggca tcggtcagtt catcggtctg catcgcccaa
     2581 aagttgacgg cgttcaacgc gaatacctgg ccatcggcta caaagacaac gacacacttt
     2641 atgtccccat tcaacaagcc gaccgcctta cccgctacat cggcgcggac agtgccaagc
     2701 ccaccttgca acgtttgggt ggcagcgact ggatgctcac caaatcgcgt gtgcgcggcg
     2761 cggttgctga agtcgccact gagcttctcg acctttacgc ccgtcgcgag gcggttgcag
     2821 ggcatgcctt tagccccgac acagactggc aaaaggaact ggaagcctct ttcccctaca
     2881 tcgaaacgcc cgaccagctg gacgccatcg cccaggtaaa agcggacatg gaaagcgacc
     2941 gccccatgga ccggcttttg tgcggtgatg tgggctatgg caaaaccgaa gtcgccttac
     3001 gggctgcttt caaagctgtg gctgatggca aacaggtcgc cgtgctcgtg cccactactg
     3061 tgctcgccca acagcattat gatactttcc gtgaacgttt gagtgtattc cctgtcaacg
     3121 ttgaaatgct ctcacgcttt agaacgccca aagaacaaga cgaaattatc caaaaactgg
     3181 ccgataaaaa gctggatatc gtcatcggca cccaccgcct gctttcagct gacgttcaat
     3241 tcgccgacct gggtttggtc attattgatg aagaacagcg ctttggcgtg acccacaaag
     3301 aacatctcaa aaaactgcgc tcggaagttg acgtgctcac cctcaccgcc acgcctatcc
     3361 cgcgcacgct ttacatggcg cttagcggtg tgcgcgatat ttccaccatc aactcaccac
     3421 ccgaagaacg aatgcccgtg ctcacccacg ttggacccta cgaccccaaa ctggtacgcg
     3481 aagccatcat tcgtgaactc gaccgtggcg gacaagtctt ttttgtgcat aaccgtgtgc
     3541 agtcgattca tgtcatctac aaccaactca gcgcgcttgt acccgaagcc cgtattgccg
     3601 ttggccatgg acaaatgccc gaaaaacaac tcgcccaggt catggaagat ttcacccatg
     3661 gagaaattga cgtcctgctt tcgactacca ttattgaaag tggcttggac atccccaacg
     3721 ccaacaccct cattattgac cgcgccgata cctttggttt ggctcagctc tatcaactgc
     3781 gtggtcgcgt tgggcgtggc gcgcaaaggg catatgccta tctcttccgc gaccgtcgtc
     3841 gcgcgccaac accggaaggc gaagaacggc ttgaaatcct cgccgagaac gcgcgcctgg
     3901 gtgccggtta ttcgattgcc atgcgcgacc ttgaaatgcg tggggcgggc gaccttctcg
     3961 gcacgcggca atccggttat atcagcgcgg tgggctttca actctatacc cgcctgctcg
     4021 gtcaggcggt gcgccgtttg cgccaaacag ccgaccccaa aagcctgccc aaattacccg
     4081 cgttggctct ttcagaactt cccagcctgg atatcaacgt tgaactcccc cttggcgctg
     4141 aaattccgcc cgattatatc cccgaccaag ccttgcgcct gacgctttac cggcgnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaaatt ccgcccgatt atatccccga
     4321 ccaagccttg cgcctgacgc tttaccggcg catcagtgca ttgcgcgatg aaaaaagtgt
     4381 tgacgaaacc gccactgaat ttgctgatcg ctttggcgcg ctccccgatg gcatcaagga
     4441 tttgctctac caaatgcgcg ttaaaatatt aggcgaagcc cgcggactgg actcaatcgg
     4501 catagctggt caacagattg tgctctccta tcccccctta ccacagggac taaaagaccg
     4561 tggcttgcca gaactccctc cgcctgcccg ggctggtcgt aacgcctact ggatcaagcc
     4621 aaacagcctg gcgcgcgaag actggcaaga tttgctcctg gatgttctgc gccaaatccc
     4681 gcttatggca cgctaatacc aaacatcagc ggcagaaaaa ccacctgttt aaaatctgat
     4741 aaaatttctt tatgatggat gaatcggtag agacgaaaga aaacccgcct gacgataaaa
     4801 agcgaattaa acccttttgg ctttgggctc tttctgtctg tctaattgca gtgggtatca
     4861 tcagcctgtt ttttgctttc taccgcgaat acatgcttgt tgtcgatgac atcccctata
     4921 ccttaaaaag tatcgccctt cgcggtcgca tagtcctgaa aaacgctggg cttgaaattg
     4981 atgatgccga cagggtctcc attgatatca acacctattc atgggaatta cctggcgaaa
     5041 tcaaaatcga ccgcgcgcga aaagtgacta ttttgcatca atctgaaacg catcaactga
     5101 actctgccgc gcgtcaccca tcaacgctac tacaactggc gggcatcaaa ctttacccca
     5161 acgacatcat cttgcagaac aagcagccta tcgaccccta cgcgccgatt ccccttgggc
     5221 aagaaataat aatcgagtac attcccgcaa aactcgtttt actcaacgac aacggacaga
     5281 tgcgaagttt ttccagccag gcagaaacgc tcgagcaagc ctttgctgaa caaaacattc
     5341 aaatcaacga acacgaccga ctctcgctac ccctttccac aaaactatcc cttgaaacac
     5401 aggtggagat tcgccgggcg cgcgaaatta ccgtcatgcg tggcgaaaac gcctttgttg
     5461 gttacagcgc tgctgacaac gcggaagaag ccttgctgga aatcggcctt cccgtccaaa
     5521 atcttgactt tatcgcgcca tcaggcgtgt tggcatttga agaaaacaat ccaatcctca
     5581 gcctggacat cgtccaggtt agcgagagtt tcaatttcat caaagaagaa accgcctatt
     5641 ccaacacgta cgaactggac ccgaacacag agttagacac cacaaccgtc ctcgtgcccg
     5701 gacaaaccgg ttacgttgtc acacgcacca tcagcatcag cgaaaatggt gaaacggtca
     5761 aaacataccc cagtcaacaa tggaaagcca gcgaccccgt taacggcata ataggccggg
     5821 gcacgatgcc cgttgtcaaa acggaaaccg tcgatggtat caccctggaa tactggcgca
     5881 aagtcaccgt ttatgccacg tcatatcatc cgtcagaatt cccccctggc gcgcgaacgc
     5941 gctccggcac accagtcacc aaaggcatta tcgccgtttc cgccgcctgg tatcccctga
     6001 tggctggaca acaggtttat gtccctggct atggccatgg cgttatcggc gactccggcg
     6061 ggggcattcc cggacgctac tggattgacc ttgcctacga tgatgaaaat tacgttggct
     6121 ggcatcactg gacgacgctt tacttcctga cgccagtgcc agcatatatc ccggcggtgc
     6181 ttccttgaaa tttctcgaca cccacgccct aatccggaaa tacgatattc gacccaaaaa
     6241 atctctggga caaaactttt tagtcgaacc gtcaggtttg cgcaaagttt tgcaagctgc
     6301 ggaattaaag ggcgacgaac aagtgctgga aatcggcgct ggattaggca gtttgaccgt
     6361 gctcctcgcc caaaacgcgc gggaagtcat cgccatagaa atcgaccaga ccctattccc
     6421 cgctctgcga gaagccatta gcaaatacaa gaacgttaaa accacacaag gcgacatcct
     6481 tgaactggac ctaaaaaacc tgctaagcga tgaacccttt gtggttgttg ccaacatccc
     6541 ctactacatc acatccgcca tcattcgcaa actactggaa gcgcaaaaac gtcccagcca
     6601 catgatacta accattcaaa aagaagtcgc tgaacgcatc atcgcccgcg acggtaaaat
     6661 gagtttattg gcgctctcag tgcaggtcta tggcaaagcc gagttggtgg caagcatccc
     6721 tgccggcgcg ttctatccca aaccggatgt cgattccgcg gtgctcaagc tctctcttta
     6781 cgacaaaccc atcattgatg aagaaaaatt gtccgtcttt ttcgaattag ccaccgcggg
     6841 attctcgcaa aggcgcaaaa ctttacgcaa taccatctct gccggcatgg caatatcaaa
     6901 ccaacaagct gaagaaatgc ttaccagcgc cggaatcgac cccaaacgcc gtgctgaaac
     6961 gctcagtctg gaagaatgga aagaactcgt cagcgtttat tgcgacacac cttccatagc
     7021 caccagtatt aaacaaaagc agaaaaatta attgcgaaca ggggatttac tcggcacgcc
     7081 aaccgcgcgg ggaaattcag gcggtgttgg cttcactttt tcaattacca ccaaataacg
     7141 ctcaccgtcc acctctggta gttctaccgg gataatctca agcaattccc ccccaaacaa
     7201 acgaatcgcg ttgcgcgcgg catcggcttc ttccatcgcg ctgtcaccct tttgcaggat
     7261 ggtacgcccg cctaccttga ctaagggcaa cagatactcc accaaagtcg gcataccagc
     7321 caccgcgcgt gccagggcaa tatcataaaa ttcacgatgc tcccagtcct gtccgatcgt
     7381 ttccgcgcgt tcagatgaaa ccagcacccg tttcaaaccc aacacccgcg ttaattcctc
     7441 gcaaaagttg gctttctttt ttatcgattc caccaaggtt aaaaatgaat ttgggtaagc
     7501 aattttcaaa ggcacgcctg gaaaccccgc cccagtaccg atatcaatca tcgctggcgt
     7561 gtcgagttcc tcgggaaagc ctaatacaca agaaagcgaa tccaaaaaat gctttatgcg
     7621 aatgccatca aggtcgcgaa tggcggtcag attgaaccgc gcgttccagt ccgttaacat
     7681 cgtttcgtaa tcagcaaact gcgccaactg cgcgtcattc agcgcgatgc cataaagcgc
     7741 ttcggcgtac tcataccagc gtgatgtcat ctattacacg tcccgtgcca aagccttcag
     7801 ccgcgcaatg accatctctg ccgccacggt gttcataccc ccttcgggga taatcacatc
     7861 cgcgaatcgt ttgctgggtt ctacaaactc cagataactc acccgaacag ttgtcagata
     7921 ttgctcgata atcgaatcca ctgttcgtcc gcgttccacc acgtctcttt gcaagcgacg
     7981 gataaaacac agttcctgtt ccgtatccac aaacagtttc atatcgaata attttcgcaa
     8041 agatggttca gcaaaaatca gaataccttc caccaaaata atcggttttg gctcaattct
     8101 caccgtctca ggcgcgcgcg tatgcaattt gtaatcatag acaggtaaat caatcgctgt
     8161 accagcacgc aaatgatgaa tatgctgaat cagcaattcc gtttccagcg agtcgggatg
     8221 gtcatagttc accttcactc gctcttcaaa agattttgaa ctttggtcac ggtagtaggc
     8281 atcgtgtggc aaaacagcga ttttatcttc gccaatctcg cgcacaatct ctttgcacag
     8341 ggtcgtcttg cctgaaccag acgcgccggc aacaccaatt acaaaactct tctttttcgc
     8401 ttgcataacg caattatact tgcaattcat gtataattac aggtgaaagt aattcgcaat
     8461 tcatgcataa aacgacgaaa tttaacctgt acgcaactaa aaaatggaga attgcaatga
     8521 attgccaagc catggagacc aaaacatgac atcaaacatt attgacggga aaaaactttc
     8581 ccaatccatt cgcgaagaca tcaaaaagca aattgaaaca cgcatcgccc agggcaaaag
     8641 ccaacctggc ttagccacca ttttggttgg tgatgaccct gcttcgcgaa cctatgtgcg
     8701 caataaactt cgcgcctgtg atgctgttgg catccgcaac atcagctacg aactgcccca
     8761 ggacaccagt caggaagaac tggacgcgct catcaaacaa ctaaacaaca acccagatgt
     8821 aaccggcatt ctcttgcaac tgcccctgcc caaacatctg gatgaagaat cccttttggc
     8881 tgcgattaaa ctgatcaaag acgttgacgg tttcaatccc atcaatgtcg gccgcatcgc
     8941 gctgcgtggc attgcccctg tctttgtgcc ttgcacacct gccggcatcc tggaactttt
     9001 gcgcgaatac gaggccccta tcgctggtgc caacgcggta gtcgtggggc gttcaaacat
     9061 cgtcggtatg ccacttgctt tgctgctgac caaagaaaac gcgaccgtca ccatctgcca
     9121 tagcaaaaca aaaaacctac ccgaaattac ccgccaggcc gacattctaa ttgtcgccat
     9181 aggcaaaccc gagttcatta ccgctgacat ggtcaaacct ggtgcttacg tcattgacgt
     9241 cggcatcaat tcagttgacg actccagccg caaatccggt tatcgtctgg taggcgacgt
     9301 cgcctttgac gaagtcgctc aagtcgccaa agcgataacc cccgtgcctg gtggcgttgg
     9361 ccccatgacc atcgccatgt tgctcaaaaa caccatgcgc gccctggaac tcgctgaaca
     9421 ataaacataa cgtcgccttc aagacggcgg attgtaaata atttttctaa aggagataag
     9481 ctctatgcaa aaaaaaggtg ttgcagtcgt tgccgttggt ggcaacgctc tcataaaaga
     9541 taaagaccat gaaacaatcc aagatcagtt cgtggccgcc tcagacacaa ccgctcacat
     9601 tgcggatatg attttagccg gttgggacgt ggtcatcacc cacggaaatg gcccccaggt
     9661 tggtttcaac ttgcgccgtt ccgaactcgc caaagacgaa gtctttgaag ttccccttga
     9721 ctactgcggc gcggataccc aaggctccat tggttatatg ttcgcccagg ctttgcataa
     9781 cgagttccgt cgtcgtggca ttgataaaaa agctgttgcg gtcgtcaccc aaaccatcgt
     9841 cgataaaaat gacccagcct tcgaaaaccc cactaaacca attggctcgt ttatggatga
     9901 agccaccgct aaagaacgcg ctgctgaaca tggctggact gttgttgaag atgccg
//
LOCUS       DAOSFC010000014         9479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_6152_length_9479_cov_4.661184, whole genome shotgun sequence.
ACCESSION   DAOSFC010000014 DAOSFC010000000
VERSION     DAOSFC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9479
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6152_length_9479_cov_4.661184"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK791_00825"
     CDS             complement(<1..267)
                     /locus_tag="PK791_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH91890.1"
                     /translation="MAKEIVLALGSGGVRGCAHIGAYRCLLDHGYKIKGISGSSAGGL
                     FGAAFASGADLDIIEKTVLDFFKNPDFSRRRGDVAFGGTGTLQEA"
     gene            422..1582
                     /locus_tag="PK791_00830"
     CDS             422..1582
                     /locus_tag="PK791_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOH91891.1"
                     /translation="MDKIHDTQAIKTAKKKSLFWRILGYIFLSLFLVMVIFFAGYQLF
                     LKPMMRKPISPKLKQELDPLKTQIVAYYQEEKEEVKEELINEIEFPEHLEEPPLFLEV
                     DEYTDTDETPRVCGMPKDLVFLLVGVDSKKDWTYRAGLADVIRLIRVDFVNKKINMIA
                     LPRDMLVDFPEGRASIESPMKINQAYTIGTAGAQQYLGEGNGAHSLAEAIDFNFGVSV
                     DHYMVVNFKFVINVIDEVGGLDVYLPQEVEDDYFGYYPAGPQHLDGIESLVLMRIRMK
                     YTDDFRVGNQTIILKAMFEKMKRPGMILRLPGLINQFRENVLTDLSVEELIRLGNCLL
                     TEFEIEDINARQFTKEHIKPAREYIPSMKLYLFVYKWGDEAVEYIHNTLMGK"
     gene            1625..2557
                     /locus_tag="PK791_00835"
     CDS             1625..2557
                     /locus_tag="PK791_00835"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH91892.1"
                     /translation="MYNCTKEFKMQDKVIGYIGLGTMGSAMAGHLLSAGFPLIVWNRT
                     ATKAQALLEKGAIWAESPAVLAAQCDVVFINVSDTKDVLEVIFGENGVAQGIRPGTIL
                     IDNSTIAPTGSREIAERLAPMGVRCLDAPVSGGDIGARNATLTIMVGGDEATLEEVRP
                     YLNVLGKKITHVGGSGAGQIAKAANQIMVAAQMVAEAELMIFAQKAGADPAKVVEAIN
                     AGAAQCWTLDVKAPKLLNGEREPGFKATLQAKDMNIAVETARAYHMNLPATALHTQLY
                     NGMVAIGGGNLDNSAIISMIEVMNGEKLKTKESD"
     gene            2639..3850
                     /locus_tag="PK791_00840"
     CDS             2639..3850
                     /locus_tag="PK791_00840"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:NF003160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOH91893.1"
                     /translation="MDFNLIKPLLKEDAKGKIVLLVMDGLGGLPLTPDGKTELETAKT
                     PNMDKLASQAELGLHHSVPFGITPGSGQAHLGLFGYDPVEYEIGRGVLSALGIDFDLG
                     PNDVAARGNFCTVDENGVITDRRAGRIPTEEGQRLCNLLKEKINLPGVELFLEPEKEY
                     RFVFVLRGEGLDGHVSDTDPQAVGKKAHIAEAQKPEAEKMAELVREFVRQGNEILKNE
                     HPANSFVLRGFAGMPKWPKFNDIWGLNSVAIAMYPMYRGVAKVVGMTVLPPAESMSEE
                     IDMLEKYWNEHDFFFVHIKKTDSYGEDGNFDGKVGIIEEVDALIPRIMALDPDVLIIT
                     GDHSTPSKLKAHSWHTIPTMIWSKNARPDGINEFGERACLRGSLGSRFDANDLMPLAL
                     SHALRIEKFGA"
     gene            3958..4977
                     /gene="amrS"
                     /locus_tag="PK791_00845"
     CDS             3958..4977
                     /gene="amrS"
                     /locus_tag="PK791_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOH91894.1"
                     /translation="MNREEKKEAKLWQALPDGRVRCELCAHYCIIPEGKKGICKERQN
                     IGGILYSLNFGHLIAAHLDPIEKKPLYHFLPGSNSFSLAMPGCNLRCAWCQNWDISQA
                     NERNDPSRLKYVHPEDVFKQVLRLKAKSIAYTYTEPTVFFEYTLELSKMSREEGIKNV
                     YVSNGFMSEQMLDLYLPWLDAANIDIKAFDDAVYRKYTGARLLEVLDNCKRIKEAGVW
                     LEVTTLLVPGVNDDHKQIEGLATFILQELGEETPWHISRYFPQAQFNKIGATPVKTVF
                     EAMSIGKCVGLKHIYAGNMSGAADTYCPYCETALILRDRIFMQENRLENGHCPKCGNQ
                     IAGVW"
     gene            complement(5011..>6546)
                     /gene="hutH"
                     /locus_tag="PK791_00850"
     CDS             complement(5011..>6546)
                     /gene="hutH"
                     /locus_tag="PK791_00850"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOH91895.1"
                     /translation="HVKQVIIDGQSLTIETVIEVAHDPAIHVNLSNEAVERINKASEA
                     VANFIETGQIAYGITTGFGAFKDRLIPLEDVEKLQENIILSHAVGVGDALDEATTRAM
                     MLIRANTLAKGHSGIRLQVLELLLSLLNHNVLPLIPEKGSLGASGDLAPLAHMSLPLI
                     GQGYVIFEGELRPSLEVLDELGLSPVRLAAKEGLALTNGTTLMTAIGTLMVHHAENIM
                     RSANLAAAITLEAQNGTTRAYTHQIHAIRPQNGQMETAAMMRDLLKNSNLTRPDDPTN
                     IQDAYTLRCVPQVHGAVSHTLSFVRGVIETELNSVTDNPLLFWDENDQPFIVSGGNFH
                     GEPLALALDYLAIAFADLGNMSERRIAKLIDSKPESNTYPDFLIAKGGVNSGFMLLQY
                     TAAALASENKSLAHPSSLDSIPSSGNVEDHVSMGATAAVHCRQVMQNVAMIIAIELMS
                     ACQAVDFRLQNLPNATMGDGTSQAYKLLRRHVPFFEADTWFQPYMLTVSDMVWEGEFA
                     KLI"
     assembly_gap    6547..6646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6721..7233)
                     /locus_tag="PK791_00855"
     CDS             complement(6721..7233)
                     /locus_tag="PK791_00855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH91896.1"
                     /translation="MPAKQWNKAPDFSLDLKKKYFATFKTDKGDIKVELFATKVPNTV
                     NNFVFLANEGFYDNTIFHRVINDFMAQGGDPTGTGMGGPGYKFRDEFDPTLRHNKPGV
                     LSMANAGPNTNGSQFFITHVATPWLDNKHSVFGEVIEGLDVLMSIPPRDPQLRGAPAV
                     TIKTIEITEE"
     gene            7388..8167
                     /locus_tag="PK791_00860"
     CDS             7388..8167
                     /locus_tag="PK791_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91897.1"
                     /translation="MTSKKSSNLSIILYVLIYLLCLTMVSLHSIAGLHISAYWGRGFD
                     AMRLDTSMINGVEITSCKCPMFFDEEEGTEVYISIKNKDTRRQTAGVVLLISKLGKRY
                     DQLQIAERMHLDAGEKRTLSWQVDTTNLVDGKVSLRLFLGQSPDHPAHSSRACLILPW
                     RGPFSANFMNTWAYPLLVLLFILSAVWVYLKSGLDWTNRKRFFFFVFANVVVILMFLS
                     IMIKFYNMVLLALPFLVLGIIATLQTSPYIPVEEYEKYIKS"
     gene            8437..9465
                     /gene="glpX"
                     /locus_tag="PK791_00865"
     CDS             8437..9465
                     /gene="glpX"
                     /locus_tag="PK791_00865"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HOH91898.1"
                     /translation="MNTAQVMKNDLDLYKSISWQLLEVTQNAAIAAARAAGHGDKNLA
                     DQAAVDAMRKTLAGMKGIRSTIKIGEGERDEAPMLYIGEELGEGMIHLDIAVDPLENT
                     NATANLNSGAVCVLTAAPAGGLVTASDTYMDKLCVGKDAADYVDIRLAPEENVYRLAK
                     ALKRRDNRELTITVLDRPRNEELINRLRATGARVRLILDGDLMPCIFSCFKGVGVHAV
                     MGIGAAPEGVISAGAIKLLGGKMQAVWKPKNDADRERLEEMGIKLDEVYSHNDLAPSD
                     DIIFCATAVTPVLTTSKAVLEGVSFFPGGAKTSSLIITKHFMNFVPTTHVLNRETFDQ
                     EKSEFRLY"
BASE COUNT         2688 a         1783 c         2275 g         2633 t
ORIGIN      
        1 cgcttcctgg agtgtgcctg taccaccgaa ggcaacatca cctctacgac ggctaaaatc
       61 gggatttttg aaaaagtcaa gaactgtttt ttcaattatg tctaaatcag cgcctgacgc
      121 gaaagccgcg ccaaacaatc cgcccgcgct tgagccagag atacctttaa ttttataacc
      181 atggtccagt agacaccgat aggcaccaat atgcgcgcaa ccacgcactc cgccacttcc
      241 taacgctaat acgatttctt tcgccatagc aacaacaacc tttctaacac taatcttaac
      301 tctattgtaa atcaaaacat attatgtaaa tgaatgggtg atataaaaaa tcaatatcct
      361 gggatagagt agaatcaaag gtgcaaagca tctattgtag agaatgcttt tggagtttac
      421 tatggacaag atccatgata cacaagcgat aaaaacagcc aaaaagaaaa gtctgttttg
      481 gcgaatactt ggctatatat tcctgtcttt atttctggta atggtcattt tctttgctgg
      541 ctatcaactt ttcttgaaac cgatgatgag aaaaccaatt agtcctaaac taaagcagga
      601 acttgaccca ctaaaaactc aaattgttgc ctactatcag gaagagaaag aagaagtcaa
      661 agaagaactc ataaatgaaa ttgaatttcc tgaacatctt gaagaacctc cgcttttttt
      721 agaagtggat gaatataccg atactgatga gacaccacgc gtttgcggta tgcccaaaga
      781 tttggttttt ctgttggttg gcgttgattc aaagaaagat tggacctatc gcgcaggatt
      841 agctgatgtc atccgattaa tccgcgttga ttttgtaaac aagaaaatca atatgattgc
      901 acttccgcgc gatatgcttg ttgatttccc tgaaggccgc gcaagcattg aaagccccat
      961 gaaaatcaac caggcttata caatcggaac tgctggagcc caacaatatc tcggtgaagg
     1021 gaatggcgcg cattcgctcg cggaagccat tgacttcaat tttggcgtaa gtgttgacca
     1081 ttatatggtc gtgaacttta aatttgttat taatgtgata gatgaagttg gtggtttgga
     1141 tgtttatttg cctcaggaag tagaagatga ttattttggc tattatcctg ctgggccgca
     1201 gcatcttgat gggatcgaat ctttggtttt gatgcgcatt cgcatgaaat atacagacga
     1261 ttttcgggtt ggaaatcaaa cgattatcct gaaagctatg tttgaaaaaa tgaaaagacc
     1321 tggcatgatt ttgagattgc caggtttaat caatcaattt cgggaaaatg ttttgacgga
     1381 tttgtcggtt gaagaattga ttcgactggg taattgcctc ttaactgaat ttgaaattga
     1441 ggatattaat gcacggcagt ttacaaaaga acatattaaa ccagcacggg aatacattcc
     1501 atctatgaaa ctatatcttt tcgtctataa atggggtgat gaggcagtcg agtatattca
     1561 caacacgctg atgggtaaat aaatacgttg taatgttacc aagctgctaa cgttgagatc
     1621 gggaatgtat aattgcacca aggagtttaa gatgcaagat aaggttattg gttacatcgg
     1681 tttaggcacg atgggcagtg ctatggcggg acatttgctt tcggcaggat tcccattgat
     1741 cgtttggaat agaacagcaa caaaggcaca agcattactc gaaaaaggcg caatatgggc
     1801 tgagagcccg gcagttttgg ctgctcagtg cgatgtggtg tttataaatg tatcagatac
     1861 aaaggatgtg cttgaagtta tctttggtga aaatggcgtg gcacaaggca tcagacccgg
     1921 cacaatattg attgataact cgacaatagc gccgactgga agtcgcgaaa tcgctgagag
     1981 attagcgcca atgggtgtgc ggtgtttgga cgcgccagtt agtggcggtg acattggcgc
     2041 gcgaaatgca actttaacga ttatggttgg cggtgatgaa gctacattgg aagaagtgcg
     2101 cccttatctt aacgttttag gtaaaaagat tacacatgtt ggtggatctg gagcaggtca
     2161 aattgctaag gctgcaaatc aaatcatggt tgccgcacaa atggtggcag aagcggaatt
     2221 gatgattttc gcacagaaag ctggtgcaga ccctgctaaa gttgtagagg ctatcaatgc
     2281 cggcgctgca cagtgctgga ctttggacgt aaaagcgcca aagttattga atggcgaacg
     2341 tgaacctggt tttaaagcga cattgcaagc gaaagatatg aacatcgcag tggaaactgc
     2401 cagggcttat catatgaatc taccggctac agcattacac acacaacttt acaatggtat
     2461 ggtcgctatc ggcggaggga acttagataa ttcagcgatt atcagcatga ttgaagttat
     2521 gaatggtgaa aaattaaaga caaaagagtc tgattagggt catattgatg tagctgacaa
     2581 ttgcaatagt atggaaaaaa ctctataatg gattggattt attttcttgg aggaagatat
     2641 ggattttaac ttgattaaac ccctgttgaa agaagatgcc aaaggaaaaa ttgttctttt
     2701 ggtgatggac ggtttgggtg gcttacccct gacacccgac ggcaaaactg aattggaaac
     2761 tgctaaaaca cctaatatgg ataaacttgc cagccaggct gaacttggtt tgcatcattc
     2821 tgttcccttt ggtattacac ctggaagcgg acaggctcat ctgggcttat ttggatatga
     2881 tcctgttgaa tatgaaatcg gtcgcggtgt tttgtcagct ttaggtatcg attttgacct
     2941 tggcccaaac gatgtagcgg ctcggggcaa cttctgcacg gtcgatgaaa acggtgttat
     3001 tacggataga cgcgcggggc gtattcccac ggaagaagga caacgcttat gcaatttgct
     3061 taaggaaaag attaatttgc ctggtgtaga actcttctta gagcctgaaa aggaatatcg
     3121 tttcgtcttt gttttgcgtg gtgaaggctt agatggccac gttagcgata cagacccaca
     3181 ggctgttgga aaaaaagcac atatcgctga agcacaaaaa cctgaagcgg aaaagatggc
     3241 agaactggta cgtgaattcg tccgtcaggg aaatgaaatt ctcaaaaatg aacatcctgc
     3301 caacagtttt gtcttacgcg gttttgccgg aatgccaaaa tggcctaaat ttaacgatat
     3361 ctggggattg aattctgttg ccatagccat gtatccgatg tatcgtggcg tcgcgaaagt
     3421 tgtcggtatg acggttttac ctccagctga gtcgatgagt gaagaaattg atatgttgga
     3481 aaaatactgg aatgaacacg atttcttctt tgtgcacatc aagaagactg acagttatgg
     3541 cgaagatggc aattttgatg gaaaggttgg catcattgag gaagttgatg ccttaattcc
     3601 cagaattatg gcacttgacc cggatgtttt gattatcacg ggcgatcatt ccacgccctc
     3661 aaaactcaag gcgcattcat ggcacactat tccgacgatg atttggtcca aaaatgcacg
     3721 cccagatggt atcaatgaat ttggcgaacg agcttgtttg cgtggtagtt tgggatcacg
     3781 ctttgacgcg aacgatttga tgcctctggc tctttcgcac gctttgcgga ttgagaaatt
     3841 cggcgcctaa agcaaatgaa attaaattat ctaaaaacgc cttgcgacgc cgcagggcgt
     3901 tttgccttaa aagaataggc aatattcatg ctacaatttt gatcaaggaa aacaactatg
     3961 aatcgcgaag aaaagaaaga agctaagctg tggcaagcac tgcctgatgg tcgtgtgcga
     4021 tgcgaattat gtgcgcatta ctgtataatt ccagaaggca agaaaggtat ttgtaaggaa
     4081 aggcaaaata ttgggggcat attgtatagc ttgaattttg ggcacttgat tgctgcccat
     4141 cttgacccga ttgaaaagaa acctttgtat cactttttgc cgggcagcaa cagtttttct
     4201 ttggctatgc cgggatgtaa tttgcgctgc gcgtggtgtc agaactggga catttctcag
     4261 gcgaatgaac gcaatgaccc aagccgattg aaatatgtgc atcctgaaga tgtttttaaa
     4321 caggtattac gcttaaaagc aaaaagtatt gcttatacat acactgaacc gaccgttttc
     4381 tttgagtaca ctttggaatt gtcaaaaatg tcgcgggaag aaggtataaa gaatgtttat
     4441 gtgagcaatg gatttatgtc tgagcaaatg ttagatttgt atttgccatg gctggatgca
     4501 gcgaacattg acataaaagc ttttgatgat gcggtttatc ggaaatatac tggcgcacgc
     4561 ttgctagaag tcctagataa ttgcaaacgc atcaaggagg ctggcgtgtg gttggaggtg
     4621 accacattat tagttccagg tgtaaatgac gaccacaaac agattgaagg gctggcaaca
     4681 tttatcctgc aagaactggg cgaagaaaca ccctggcata tcagccgcta ttttccacag
     4741 gcacagttta ataaaattgg cgcaacacca gtaaaaacgg tgtttgaagc gatgtcaatt
     4801 ggaaagtgtg tcggactaaa acatatttat gctggcaata tgtctggtgc agcggatact
     4861 tattgtccgt attgtgaaac agcgcttata ttacgtgacc gcatttttat gcaggaaaac
     4921 cgacttgaga atggtcactg tcccaagtgt ggaaatcaaa tcgccggcgt atggtagggc
     4981 cggcgatcaa tcgtttgttc cagatagaga ttagatcagt ttggcgaatt cgccttccca
     5041 taccatgtca gaaacagtga gcatataggg ctggaaccat gtatcagctt caaagaaagg
     5101 cacatgcctg cgtaaaagct tataggcttg gcttgttcca tcacccatgg tggcgtttgg
     5161 caaattctgc aatctaaaat caaccgcctg gcaggcactc atcaactcaa tggcaataat
     5221 catcgccaca ttttgcataa cctggcgaca atgcacggca gcggtcgcac ccatggagac
     5281 atggtcttca acattgccgg aactggggat gctgtctaaa ctgctgggat gggccagcga
     5341 tttattctcg gaagccaagg ctgcggcagt gtattgtaaa agcataaagc ctgaattaac
     5401 accaccttta gctatcagga aatccgggta agtattcgat tcaggtttgc tgtcaatcag
     5461 tttggcgatg cgtcgttcag acatgttccc taaatctgca aaagcgatgg caagatagtc
     5521 caaagctagc gcaagtggct caccatggaa attgccacca gaaacgataa atggctggtc
     5581 attttcatcc cagaagagaa gcgggttgtc ggtaacggag ttgagttcgg tttcaataac
     5641 gccacgtaca aaggacaatg tatggctaac cgcgccatgt acctgtggaa cacagcgtaa
     5701 tgtgtaagcg tcctggatat tggtggggtc atcggggcgg gtgagattac tgtttttcag
     5761 taaatcacgc atcatcgcag cagtttccat ttgaccgttt tgggggcgaa tggcgtggat
     5821 ttggtgcgtg taagcccgag tggtaccgtt ttgggcttcc aacgtgatag cggcagcgag
     5881 attagcactg cgcataatgt tttcagcgtg atgtaccatg agtgttccga tagcagtcat
     5941 caaagttgtg ccattggtga gcgcgagtcc ttctttggca gccaggcgaa ctggactaag
     6001 tccgagttca tcaaggactt ccagagatgg acgtaattca ccttcaaaga tgacgtagcc
     6061 ttgacctatc agtggcaaag acatgtgggc taagggtgcc aggtcgccac tggcgccaag
     6121 tgaacctttt tctggaatga gtggaagtac attatggttt agcagtgaaa gcaataactc
     6181 taaaacttgc aggcgaatac ccgaatgacc ttttgcaagg gtgttggcgc ggatgagcat
     6241 catagcccgg gttgtggctt catcaagcgc gtcaccaacg ccgaccgcat ggctaagaat
     6301 aatgttctcc tgcaactttt caacatcttc aagtgggatg aggcggtctt taaacgcgcc
     6361 gaaacccgtg gtaatgccat aggcaatttg cccggtttcg atgaagtttg cgaccgcttc
     6421 tgaagccttg ttgatgcgtt caaccgcttc attgctaagg ttcacatgga ttgcggggtc
     6481 gtgggcgact tcgataactg tttcaatcgt gagagattga ccgtcaatga tgacttgttt
     6541 cacgtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncgat aactgtttca
     6661 atcgtgagag attgaccgtc aatgatgact tgtttcacgt gactttcctt tcgtagaagc
     6721 ttattcttcg gtaatttcaa ttgttttaat ggtcacagca ggcgcaccac gaagttgtgg
     6781 gtcgcgtggg ggaatggaca tgagcacgtc cagaccttct atgacttctc caaagacgct
     6841 gtgtttattg tccaaccagg gtgtggctac gtgggtaatg aagaattgcg aaccgtttgt
     6901 gttgggtcct gcatttgcca tcgataggac gccaggtttg ttatgacgta gtgttgggtc
     6961 aaattcatcg cggaatttgt agccaggtcc acccatgcct gtaccggtag gatcgccacc
     7021 ttgtgccata aagtcgttga ttacacggtg gaagatggtg ttgtcataaa acccttcatt
     7081 cgcaaggaaa acaaagttgt taactgtgtt gggtacctta gtggcgaaaa gttcgacttt
     7141 tatgtcgcct ttatccgttt tgaatgtagc aaaatatttt ttctttagat caagtgagaa
     7201 gtcaggtgct ttgttccatt gttttgctgg catagtttgt ctccgtttta ggttttcttg
     7261 attataacaa tattgtcacg tttcaacagg aattaatcag ctgctggttt cttcactggt
     7321 ttaatgataa agcatgtaag cttaaaaacg actttatatt tatctaaagg aaaagtataa
     7381 tgaaactatg acaagtaaaa aatcaagtaa tctttcaatt attttatacg tcttaattta
     7441 cttactatgt ttgacgatgg tctcattaca ttctattgcc ggattgcata tatcggcgta
     7501 ttggggacgt gggtttgacg ctatgagact agacaccagc atgattaatg gcgttgagat
     7561 tacttcctgt aaatgcccga tgttttttga tgaagaagaa ggtacagaag tttatatttc
     7621 gataaaaaac aaagataccc gccgacagac tgcgggagtg gttttgctaa tatctaaatt
     7681 gggtaagcga tatgaccagt tgcaaatagc agaaagaatg catctggatg ctggggagaa
     7741 acggactctt agctggcagg tggatactac aaacctggtt gatggaaagg tttcgttacg
     7801 tttattcctt gggcaatccc ctgatcatcc cgcacattca tcgcgtgcat gtttgatatt
     7861 accctggcgg ggtccttttt cagcaaattt tatgaatacc tgggcatatc cattgttagt
     7921 tttactgttt attctaagcg cagtatgggt atatttaaaa tcaggattag attggacaaa
     7981 tcgaaaacga tttttctttt tcgtgtttgc aaatgttgtc gtcatcctta tgtttttatc
     8041 aataatgata aagttctata atatggtttt gcttgcatta ccatttcttg tgcttggcat
     8101 tattgcaact ttgcagacca gtccatacat accggtagaa gaatacgaaa aatatattaa
     8161 gtcttaatcc ttaaggtttg cattaacagc ttgtatagct atctgatgag aggccactgt
     8221 taataaagag attagcataa aagataaatt atgcatgtta tcaacaagga ctggtataat
     8281 aagccacatc tcataaagag actgtcttga aaggcggatt gcgtgttaaa aaacagtaaa
     8341 aagccgaatc tattaatgtg tcaacccaaa agttcaacag catatttagt tgatagtgca
     8401 actaaccgta agttttttat tgtgaaagga gtatgaatga acaccgcaca agttatgaaa
     8461 aatgaccttg atttatacaa gagtatttcc tggcaattat tggaagtaac tcaaaacgca
     8521 gctattgcag cagccagggc tgctggtcac ggtgataaaa atttagctga ccaagcagca
     8581 gttgatgcca tgcgaaaaac tctggcaggt atgaagggta ttcgatcaac tattaagata
     8641 ggtgaaggcg aacgcgatga agcacctatg ctttatatcg gtgaggagct aggcgaaggt
     8701 atgatccatt tggatattgc cgttgacccc ctggagaaca caaacgctac tgctaatctt
     8761 aattcaggtg cggtgtgtgt gctgaccgct gcgccggcag gaggtctggt gaccgcttcg
     8821 gatacctata tggataaatt gtgtgttgga aaagatgctg ctgattatgt cgatattcgg
     8881 ctcgcaccag aagaaaacgt ctatcggctt gcgaaagcat taaagaggcg agataaccgg
     8941 gaattaacca tcacggtttt agatcgtccc cgtaacgaag aattaattaa tcgcttgcgg
     9001 gctactggcg cacgcgtacg tttaattctt gatggtgacc tgatgccttg cattttttca
     9061 tgtttcaaag gtgttggtgt gcatgcagtt atgggcattg gagcagcacc agaaggtgtg
     9121 atttctgctg gtgcaatcaa gcttttgggt gggaaaatgc aggcagtttg gaaaccaaaa
     9181 aatgatgcag atcgcgaaag actcgaagaa atgggtataa agcttgatga ggtctattct
     9241 cataacgatt tagcgcccag tgatgacatc atcttttgtg ctactgctgt aacgccagta
     9301 ttgacgacat ccaaggcagt gttggaaggt gtgagcttct tcccaggtgg tgccaaaaca
     9361 agcagtttga tcatcaccaa acactttatg aactttgtac ctacaacaca cgtcttgaac
     9421 cgagaaactt ttgaccagga aaagagtgaa tttaggcttt actagccaat aaattgcaa
//
LOCUS       DAOSFC010000015         9033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_6560_length_9033_cov_4.736578, whole genome shotgun sequence.
ACCESSION   DAOSFC010000015 DAOSFC010000000
VERSION     DAOSFC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6560_length_9033_cov_4.736578"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PK791_00870"
     CDS             <1..670
                     /locus_tag="PK791_00870"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91899.1"
                     /translation="TRTRLHRLAGAFIPSNLDYAKWLSVFKDLPLQMSSEHLTSLKQI
                     MRLHASTAERLPYLDEVYKDIFSHMPSPSSILDLACGLNPLAIPWMPIDESTEYHACD
                     IVLPMLDFLENYFSVFRPNTHIFEKNILEFQSERSFDLIMLLKTMPLLAQIDKTAPEK
                     LFSRLNFKYLLISYPLQSLGKQYKGMEKTYRAQFENLIANKPFQVQEYKLPNELFFIL
                     SHHD"
     gene            663..1481
                     /locus_tag="PK791_00875"
     CDS             663..1481
                     /locus_tag="PK791_00875"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91900.1"
                     /translation="MIEEAYILKLIQDVQSSRKYRDLDLPDAFLRDLIENEFLHTTNK
                     RIIKDNFRKKLHEVIAPYLEDIDYYQETKYLSAEYQQNSLNNDQIKNWARTIMHKHAS
                     TRERLANLEGFYQTIWSNIGYPSSIIDLACALDPLSLPWFNNPFLNSFYGIDIHKPRL
                     DFLETFFQLYFPFAKTIQQDFIAEPVQIKADCAFLFKEAHRMEKRKSGSTSQLIQNLD
                     VQHIIISLPAKDLSGHHDLSDFHTRLIENAIQGQNLSMTMAQVGLELLYFIEKL"
     gene            1478..2926
                     /locus_tag="PK791_00880"
     CDS             1478..2926
                     /locus_tag="PK791_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91901.1"
                     /translation="MSDVIQSTLNRYQAYLSAEDFSKLIEVSSQDAPLAIRANLLKNA
                     NPQQSLLEWQNKYGWRTDAVPFWQYAVQILQAEIAPSQTIEHQFGYYYIQDAASTLPV
                     ALFDPIDKNHLVLDMAASPGGKTTQLIDRSLDRAFIIANDSSTSRLSALRVVSQNWGM
                     ANGMISNYAAEKIGDWFPNTFDRILLDAPCSMESLRVSKSHPYRDISISERERLYSRQ
                     LELLISALKACKVGGEIVYSTCTMAPEENESVINALLTRYPNVVTISRIENQNPNSQG
                     LSHFEEQIYHPDIRHALRVWPHLYATNGFFACKLQKTDEIETAGICPPKRAFSQTGLS
                     FLKSDDLNFVLEELNIRFGFESKLLEDFEIYQRDNQLFIIPKAYLEHFPTLPFNALGL
                     PLGKRIKTAFEPSFEFVMRFGMTFQRNVWHIPADLQIPWLQGRDLHSVNLSPNCSYGI
                     VAVKTEDNILIGAGKWSAQRLRNLLPHRYLIH"
     gene            complement(2952..3701)
                     /locus_tag="PK791_00885"
     CDS             complement(2952..3701)
                     /locus_tag="PK791_00885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOH91902.1"
                     /translation="MAMQRFFIDPKYIDGNDVSFPENIEKQIRKVLRLESSDAVVVLD
                     NTGMTYLVELEVKRGQAIKGIIVAKEPGRAEPCVGLRLCFSLSRKEKLEWIFQKCTEI
                     GVTVFQPFISSRSIVQDIDSFESKRERYATIAKEAAEQSMRSVMPVILPVVSYEALVA
                     EKTDGLKIIPWEETATVERMKAETFCFEEGKEPSEIRVLIGPEGGFSREEVDQAEEYG
                     YHQVSLGDSILRMETACVVATALIRHLCETC"
     gene            3783..4505
                     /locus_tag="PK791_00890"
     CDS             3783..4505
                     /locus_tag="PK791_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOH91903.1"
                     /translation="MKQPKTRDSYKYLDLVSMIFVVVLVLSNFASSAKLIDWGFSIGN
                     IPMVFDAGTVFFPIAYVIGDILTEVYGFKRARRVIWYGFGALLFSFLSFLLIKHLPGE
                     SSWLADGGQSAFDYILGGLSSGGIVLASLAGYLGGSFSNAISMAILKNRTGGKYLWIR
                     TIGSTLLGEFLDTALFIGVATLTGVFSKDLFWSLITTNYLFKVLIEVLCTPLTYWSVN
                     KLKETEGIDVYTDPKDLNPFAF"
     gene            complement(4486..5178)
                     /gene="rsmI"
                     /locus_tag="PK791_00895"
     CDS             complement(4486..5178)
                     /gene="rsmI"
                     /locus_tag="PK791_00895"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOH91904.1"
                     /translation="MLYLVATPIGNLGDMSFRAVDVLKSVDLIASEDSRHSSILLNHY
                     EISKPQTALNNYNERKVAPKLIERMKRGETIALISDAGTPGISDPGYLLVRLALDEGI
                     DVQAIPGPSAGVMALTLSGLPMHSYLFKGFPPVKEGPRKRFLAMDAESGHTMVFYESP
                     YRLLAFLDNALEVFGDRRAALANDLTKKFEKVLRGNLSEIKEWVQEEKIRGEYVICIE
                     GLSKEELRKQKD"
     gene            complement(5181..5801)
                     /locus_tag="PK791_00900"
     CDS             complement(5181..5801)
                     /locus_tag="PK791_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOH91905.1"
                     /translation="MKKINTLIFDFGNVLVNLHSEAELRELLAQLGQPASGTMADMLL
                     MPGGSDVFWDVMRGKTTEAEMWQMIADEWNIPFDLAKKVMKDVAAPNRPNMPLIKYIE
                     GLKGDYKIGVLSNAGDATLERLLQWYPYDQFVDVFIISAEVKLAKPDAEIYELALNFL
                     NAKAEESLFVDDLIENIKAAEELGITGIWHRDNDETIARLKELLGE"
     gene            5878..7602
                     /locus_tag="PK791_00905"
     CDS             5878..7602
                     /locus_tag="PK791_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91906.1"
                     /translation="MERQVPVRSSDEIDLYLRTIYSLLRSNNEVSIRTLEEVHAGTNS
                     SLHIHARSSSKPDISALTYSLMRLPKEVIDAKKVVLGQAASSFDDFGYGNVEKWQHVS
                     APARRRRCFWDGEETLACFIASRSDIDDVLPVLTALQMEWNKLHFLLQSAPQDLIDNA
                     IPTQVKSFSELSTHLNIEIDELARLHSIWDDDFTNVLYKIKGSPLDYKVRLLSGSLND
                     YRKATVIWWDNIQNKVPEIVNRPIYFVSSNTHSLTNLLSGYALTKRDIILDYIARHEP
                     RLMNEWLEIQGQDKATNVENFFYYALRKLTETDQYASYIEEQQSHETSLGIHRVSSDH
                     SFDVEANIIQLNTLDPRFFDPRLAPKHEGYEKSWEFLKDSDALILNIDYPLGASAYNL
                     LTKIAENVEKILGIYIMGKAASLNAIRGDVIIPNVVYDEHSKNSYLFSNAFKVTDVAS
                     YLNFGTVLDSQKAVTVLGTYLQNATIMDVVYREGYTDIEMEAGPYLSAIYELFRPARH
                     PLNEIVNLYELPIDLGFLHYVSDTPFSKGQNLGAGSLSYFGMDSTYAVSLAILQRIML
                     LERRRLRE"
     gene            complement(7677..>9033)
                     /locus_tag="PK791_00910"
     CDS             complement(7677..>9033)
                     /locus_tag="PK791_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH91907.1"
                     /translation="CGSSDLHQDDDVLDTWFSSGLWPFSVFGWPEETPDYKYFYPTSI
                     LETGYDILFFWVARMVMDGIEFTGQVPFHTVYLHGMIRDEKGQKMSKTKNNVIDPLIL
                     MDEYGTDALRFTLLVGSSPGNDQNVGVKKVEANRNFANKVWNIGRYVINAIDRIETKA
                     NDEPDWTLADGWIWARLKQTVNSVNSLFEAYQYGEAGKQIYEFLWNDFADWYVEGSKR
                     QLAEESDRSAYTAYTLAFVLDTMLRLLHPFTPFVTEALWGYLKNACKESGLVYTPDED
                     WADDLMIAKWPEAMALDGKENEYLREFGLVQDIVRAIRNVRSEYRIEPNKKLKAAMVS
                     EELIALLEAQKEVIADFAHLDLDSLNVSASPIEAEGLTALVVDRVEVFLQLSSDGDDE
                     ADRERLTKELEEAESHIKRLKALLASPFAQKAPEKVVAGERQKLADYQATAEKLRERL
                     G"
BASE COUNT         2557 a         2168 c         1646 g         2662 t
ORIGIN      
        1 cacccgcaca agattgcatc gcctggcagg cgctttcatc ccgagcaact tagactacgc
       61 gaaatggttg agtgttttta aagacttacc attacaaatg tcatcagaac atttgacaag
      121 cctgaaacaa atcatgcgtt tgcatgcttc taccgcagaa cggcttccat accttgatga
      181 agtctacaag gatatcttct cacatatgcc atcaccttcc agcattcttg atctcgcctg
      241 cgggctaaat ccacttgcga ttccgtggat gcctattgat gaaagcactg agtatcatgc
      301 ctgtgatatt gttttgccta tgcttgattt tttggaaaac tatttttctg ttttcagacc
      361 gaatacacat atctttgaaa agaacattct tgaattccaa tccgaacgat cattcgacct
      421 gattatgctc ctgaaaacaa tgcctttact tgcgcaaata gacaaaactg ctcctgagaa
      481 actatttagc cgtctaaact ttaaatacct tctcatttct tatccattac aaagtttggg
      541 gaaacaatac aaaggcatgg aaaaaaccta ccgcgcccaa tttgagaacc ttattgccaa
      601 taagccattt caggttcaag agtataaact tcccaatgaa ttgttcttta tcctaagcca
      661 tcatgattga agaagcttat atcttaaaat taattcaaga cgtgcaaagt tctagaaagt
      721 atcgtgattt agacttgcca gatgcatttc ttcgagattt gattgaaaat gaatttctac
      781 ataccacaaa caaaagaata attaaagaca acttccgtaa aaagctccat gaagtcattg
      841 cgccttatct ggaagacatt gactactatc aggaaacaaa gtacctatca gcagaatacc
      901 agcaaaacag tcttaataac gaccagataa aaaactgggc acgaactatt atgcataagc
      961 atgcttccac acgcgaacgc ctggctaatc ttgaaggttt ttatcaaact atttggtcaa
     1021 acatcggata tccatcatcc atcattgatt tggcctgcgc tttggaccca ctttccctgc
     1081 catggttcaa caaccctttt cttaattctt tctatggcat tgatatccat aaaccgcgcc
     1141 ttgatttttt agagaccttt ttccagctgt attttccatt cgcgaaaaca attcaacaag
     1201 actttattgc ggaacctgtc caaattaaag cagattgcgc atttttattc aaagaagcac
     1261 atcgcatgga aaaacggaaa tcaggcagta caagccaatt gatccaaaat ctagatgttc
     1321 agcatatcat tatcagtttg ccagccaaag atcttagcgg acatcatgat ctttctgatt
     1381 tccacacgcg tcttatcgaa aatgccatcc aaggccaaaa cctttctatg accatggcac
     1441 aagtgggctt ggaactgcta tacttcattg agaaattatg agtgatgtca ttcaatccac
     1501 cttgaatcgc taccaagctt atctgtcagc tgaagacttt tccaaactga ttgaagtctc
     1561 cagccaggat gcaccgctgg caattcgtgc caacctactt aaaaatgcta atccccaaca
     1621 aagtctgctt gaatggcaaa acaaatatgg ctggcgtaca gatgctgttc cattttggca
     1681 atatgctgtt caaattctcc aggcagagat cgctcccagt caaacaattg aacaccaatt
     1741 tggttattat tacattcaag acgcagcgtc cacactgcct gtggctttat ttgatcctat
     1801 cgataaaaat catcttgttt tggatatggc cgcctcgcca ggtggcaaaa caacgcaact
     1861 tatcgaccgc agtctggatc gcgcctttat cattgctaac gattccagta cctcacgtct
     1921 ttcagcttta cgcgtggtaa gtcaaaactg gggcatggca aacggcatga ttagcaatta
     1981 tgctgctgaa aaaatcggag attggttccc caacaccttt gatcgcattt tgcttgatgc
     2041 accctgtagc atggaaagtc tgcgagtttc aaagagtcac ccctaccgcg atatcagcat
     2101 atcagaacga gaacgtcttt acagtcggca acttgaatta ctcatttccg ccctaaaagc
     2161 gtgcaaagtg ggcggggaaa ttgtttattc cacatgcact atggcgccag aagagaacga
     2221 atccgtcatc aacgctttac tcactcgata tcccaatgtt gttacaattt ccagaatcga
     2281 aaatcagaac ccaaacagcc agggactgtc acatttcgaa gaacaaatct atcatcccga
     2341 tattcgacac gcactgcgtg tttggcctca cctatacgca acgaatggtt tttttgcatg
     2401 caagctgcaa aagacggatg aaatcgagac tgctggaatt tgtccgccca aacgtgcttt
     2461 ttctcaaacc ggactatctt ttctcaaatc agatgatcta aatttcgttc tcgaagaact
     2521 caatatacgt ttcggtttcg aaagcaaatt gcttgaggac ttcgaaatct atcaacgtga
     2581 caatcagctc tttattattc ctaaagccta tcttgagcat ttcccgacct tgccattcaa
     2641 tgcccttgga ctccccttag gcaaacgcat caaaacggct tttgaaccca gttttgagtt
     2701 tgtcatgcgt tttggtatga cctttcaacg taatgtctgg catataccag cagatttaca
     2761 gattccctgg cttcagggtc gtgatctgca tagtgtaaat ctcagtccaa attgttcata
     2821 cggtatcgtt gcagtaaaaa ctgaagataa tattttgata ggtgcaggta aatggagtgc
     2881 gcaacgtctg cgcaacctat tacctcaccg atatttgatt cattaggtat tttcttagac
     2941 ctatttataa tttagcaggt ctcacagaga tggcgaatga gagcggtcgc cacaacacaa
     3001 gccgtttcca tccgcaatat tgaatcccct aacgaaactt gatgatagcc atattcttca
     3061 gcttgatcta cttcttcgcg gctaaaacca ccttctggtc caattaaaac gcgtatttcg
     3121 cttggttcct taccttcttc aaagcaaaaa gtttccgctt tcatgcgttc tactgtagct
     3181 gtttcttccc atggaattat ctttagacca tcggtttttt cagcaaccaa ggcttcgtag
     3241 ctaactacag gtaagattac cggcataact gaccgcatac tttgttcagc agcttctttt
     3301 gctatcgttg cataacgttc tcgcttcgat tcaaaactat cgatatcttg cactatactc
     3361 cggcttgaaa taaaaggttg aaagactgta acaccgattt cagtacattt ctgaaaaatc
     3421 cattccaact tttcttttct gctcaagcta aaacataagc gaagcccaac gcaaggttcc
     3481 gccctgcccg gttctttagc gacgataatc ccctttatag cttgtccacg cttgacttcc
     3541 aattcaacta aataagtcat accagtattg tcaagcacaa caactgcgtc agaactttcc
     3601 aaccgcaaaa ctttgcggat ttgcttttca atattttcag gaaaactcac atcatttcca
     3661 tcaatatact ttgggtcgat aaaaaaacgt tgcattgcca tggcacctat tgtagcctac
     3721 ttttgaaaat ttttaacaag caattttgac ttttttgtag tataacattg gacagagtgc
     3781 caatgaaaca acctaaaaca cgcgattcat ataaatatct cgaccttgtt tcaatgattt
     3841 ttgtagtcgt cttggtcttg agtaattttg catcctcagc gaaactaatt gattggggat
     3901 tctccatcgg taacatcccg atggtctttg atgcagggac tgtctttttt ccaattgcct
     3961 atgttattgg cgacattcta acggaagttt atggtttcaa acgcgcccgt agggtaatct
     4021 ggtatggttt tggcgccttg ctttttagtt tcctgtcctt tttacttatc aaacatctgc
     4081 caggtgaatc cagctggctg gctgatggtg gccaaagcgc ctttgattat attttgggcg
     4141 gacttagcag tgggggtatc gttctcgcca gtttagcagg ctatttgggt ggcagtttct
     4201 caaatgccat tagcatggcc atcctgaaaa accgaactgg ggggaaatat ttatggatta
     4261 ggactatcgg cagcacactc ctgggagaat tcctggatac cgcgctgttt atcggcgttg
     4321 ccacactaac aggcgttttc agcaaagatt tgttctggtc gctaatcacc accaactatt
     4381 tattcaaagt actcattgaa gtcctctgca ccccattgac atactggtca gtcaataaat
     4441 tgaaagaaac agaaggaatt gacgtctata ctgacccgaa agatcttaat ccttttgctt
     4501 tctgagttct tcttttgaca agccctcaat gcaaattaca tattcaccac gtatcttttc
     4561 ttcttgcacc cattctttaa tttcgctgag gtttccacgt aaaactttct caaatttctt
     4621 tgtcagatcg tttgccaatg cagcccttcg gtcaccaaag acctctagcg cattatccaa
     4681 aaaagctaac aaccgatatg ggctttcata aaaaaccatt gtatgtcctg attcagcgtc
     4741 cattgctaaa aaacgtttcc ttgggccttc ctttactggc ggaaagcctt taaaaagata
     4801 gctatgcatc ggtaaaccac ttaaggtcaa cgccatcact cccgcgctcg gaccaggaat
     4861 agcttgcaca tcaatgcctt catctagcgc cagcctgact aacaaatatc ctgggtcgga
     4921 tatgcccggt gtcccagcat cgctaattaa ggcaatcgtt tcaccacgtt tcatacgttc
     4981 aatcagttta ggcgccacct tgcgttcatt gtaattattt agcgcagttt gtggtttgct
     5041 aatttcataa tggttcagca aaatgctact gtgccgggaa tcctcactgg caattaaatc
     5101 cacacttttt agcacatcta cagcgcggaa actcatatca cctaaattac cgatgggtgt
     5161 tgcgaccaaa taaagcactt ttattctccg agtaattcct tcaatcgcgc aatggtttca
     5221 tcattgtcac gatgccaaat gccagtaatg ccaagctctt cagcagcttt gatattttcg
     5281 ataaggtcgt ctacaaacaa actttcttca gcttttgcat tcaaaaaatt caaagccagt
     5341 tcataaattt ccgcatcggg tttggcaagt ttcacttcag ctgaaataat aaaaacatca
     5401 acgaactgat catacggata ccactgtaac aagcgttcca gtgttgcatc accggcgttg
     5461 ctaagcacac caatcttata atcacctttc aaaccttcaa tgtatttaat caaaggcata
     5521 ttaggtctat ttggtgccgc aacatccttc atcacctttt ttgccaggtc aaacggtata
     5581 ttccattcat cagcaatcat ctgccacatt tctgcttcgg tcgtttttcc gcgcatcaca
     5641 tcccaaaaga cgtccgatcc gcccggcatc aaaagcatat ctgccatcgt accactggca
     5701 ggctgtccta actgcgctaa taactcgcgc aattctgctt cggagtgtaa attaacgagc
     5761 acatttccaa aatcaaaaat aagggtattg atctttttca tatgcctatt ataaggcatg
     5821 cccccttaaa aatatgcatc atgataaaat tttaagcact tatcaagaga ggtttttatg
     5881 gaaagacaag tcccagttag atcatctgat gaaattgatc tctatttacg caccatatat
     5941 tcgcttttac gttcaaacaa cgaagtgtcc atccgtacgc ttgaagaagt tcacgccggt
     6001 acaaattcat ctcttcatat ccacgcacgt agcagcagca aacctgatat atccgccttg
     6061 acttacagtc tcatgcgttt gcccaaagaa gtgatagacg ctaaaaaagt tgttttaggt
     6121 caagctgctt caagctttga tgattttggt tatggaaatg tcgaaaaatg gcaacacgtt
     6181 agcgctccag cccgaaggcg tcgatgcttt tgggatggtg aggaaacctt agcctgcttt
     6241 atcgcatcgc gttcggatat tgatgatgtt ttacctgttc tgactgcttt gcaaatggag
     6301 tggaataaac ttcatttctt acttcaatcc gcaccacaag atctcattga caatgccatt
     6361 cccacccagg taaaaagctt ttcggaactt agcactcact taaacattga aattgatgaa
     6421 ctcgcccgat tgcattccat ttgggatgat gacttcacca atgttctata taaaataaaa
     6481 ggttcacctt tagattacaa agtccggttg ctaagcggtt ctttaaacga ctatcgtaaa
     6541 gcgaccgtta tttggtggga taacattcaa aataaagtac cagagattgt gaatcgccct
     6601 atctattttg tttcatctaa tacacatagc ctcaccaatt tactctcggg ctatgcctta
     6661 acgaaacgtg atattatcct cgactacatc gcgcgccatg aaccacgtct catgaacgaa
     6721 tggcttgaaa tccagggaca agataaagcc acaaatgttg aaaacttctt ctattacgcc
     6781 ttgcgtaaac tgacagaaac tgaccaatat gccagttaca ttgaagaaca gcaaagtcat
     6841 gagacctctc ttggcattca ccgcgttagc agtgatcaca gcttcgatgt agaagccaac
     6901 atcattcaat taaacactct ggacccaaga ttttttgacc cgcgactggc acccaaacac
     6961 gaaggttatg agaaatcctg ggaattttta aaagattctg acgcactcat tttgaacatc
     7021 gactacccac tgggcgcatc agcctataac ttattgacca aaattgctga aaatgttgag
     7081 aaaattctcg gcatttacat catgggcaag gctgccagtc taaatgctat tcgtggcgat
     7141 gtcatcattc ctaatgttgt ttatgatgaa cattcgaaaa acagctattt gttctcgaat
     7201 gccttcaaag taaccgatgt tgccagctat ctcaattttg gcactgtttt ggacagccag
     7261 aaggcagtta ccgtcctggg cacctatctg cagaacgcaa ccatcatgga tgttgtttac
     7321 cgggaaggct acactgatat cgaaatggaa gcgggtccgt atcttagcgc gatttacgaa
     7381 cttttcagac ccgcccgcca cccactcaac gaaatcgtaa acttatacga actgccgatt
     7441 gatttgggct tcctgcatta tgtatcggat acacctttta gcaaaggcca aaatctcggt
     7501 gctggcagct tgtcatactt cggtatggat tcaacctatg ctgtttcttt ggctattctt
     7561 caacgcatca tgctgcttga aagacgccgc ctgagagaat aagcaagcat ctcatcttaa
     7621 aaaccaaacg cccaggtctt aacatcctgg gcgttatcat tttgtgtagt tctaaactag
     7681 cctaagcgtt cacgtaattt ttccgcagtt gcctggtagt cggctagttt ttgtctttca
     7741 ccagccacaa ccttttcagg tgctttttgc gcgaatggac ttgccaacag tgctttcagg
     7801 cgtttaatgt gtgattcagc ttcttctaat tctttggtta accgttctcg gtcagcttca
     7861 tcatcgccat cactgctcaa ttgcaggaat acttcaaccc tatccacaac caaagctgtc
     7921 aaaccttcgg cttcaatcgg tgatgcacta acattcaaac tatccaggtc aagatgagca
     7981 aaatcagcaa tgacttcttt ttgtgcttcc aataaagcaa tcaattcttc tgacaccata
     8041 gctgctttta gcttcttatt tggttcaatt ctatattcag aacgaacgtt tctaatcgcc
     8101 ctcacaatgt cctgcaccaa gccaaattcc cgcaaatatt cgttctcttt tccgtccaag
     8161 gccatcgctt caggccattt ggcgatcatc aaatcatccg cccagtcttc gtctggcgtg
     8221 taaactaacc cagattcttt gcatgcgttc ttcagatatc cccaaagcgc ctcagtaaca
     8281 aatggcgtga aggggtgcaa gagtcgcaac atcgtatcca atacaaatgc gagtgtgtac
     8341 gcggtataag cagatctgtc gctctcttct gctagctgac gttttgatcc ttccacatac
     8401 cagtcggcaa agtcattcca caagaattca taaatctgtt tgccagcttc gccatattga
     8461 taggcttcaa aaagtgagtt aacgctgttc accgtttgtt ttaagcgtgc ccaaatccaa
     8521 ccatcagcta aagtccagtc cggctcatca tttgcttttg tctcaattct gtcaatagca
     8581 tttatgacat agcgtccaat gttccaaact ttattagcaa agttacggtt ggcttcaacc
     8641 tttttaacgc caacattttg atcattgcct ggcgatgaac ccaccagaag agtaaagcgc
     8701 aatgcatccg ttccgtattc atccattaaa ataagcgggt cgataacgtt attcttggtt
     8761 ttactcatct tttggccttt ttcatcccga atcatgccat gcagataaac cgtatggaat
     8821 ggcacctgtc cagtaaactc tatgccgtcc atcaccatac gtgcaaccca gaagaacaaa
     8881 atatcataac cagtttccag gatacttgtt ggatagaaat atttgtaatc gggtgtttcc
     8941 tctggccaac cgaaaacaga aaatggccac aaaccagatg agaaccaggt atcgagtaca
     9001 tcatcatcct ggtgtaaatc tgatgaccca cac
//
LOCUS       DAOSFC010000016         8572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7038_length_8572_cov_5.069156, whole genome shotgun sequence.
ACCESSION   DAOSFC010000016 DAOSFC010000000
VERSION     DAOSFC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7038_length_8572_cov_5.069156"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="PK791_00915"
     CDS             <1..637
                     /locus_tag="PK791_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH91908.1"
                     /translation="NYVAINLSDYFSEGPWKDRSPGNIVSRLPAIQPSAEIPNIGPVP
                     VGFIVAVLTALLIWFVINRTTFGFEINSVGLNPHASKYAGMRTKLLIVAAMVVSGALA
                     GVGGAVETMGVVGRFQPGFNTGLGFDGITVALLARNNPIAVILSSLFLGAMKGGANTM
                     QFYANVPNQIIDIIQGTMLLFVTAEMVVRWVFKLRKQRDTEITVTSGWGQR"
     gene            634..1650
                     /locus_tag="PK791_00920"
     CDS             634..1650
                     /locus_tag="PK791_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH91909.1"
                     /translation="MISKTVDKKRELSINWKLLRQVILGLVIVAAILFFNARSPEKTS
                     AVLSSTLRYAVPLILGALCGIFCERAGIVNIGIEGQMLFSAFLSYMTNVWTGNLLLSL
                     LVGMLTGAILGLFLAFLSIKLKLNQTIGGTVINILATGITGYFYQTGLTTQGKLWNIP
                     LGPLAKIPLIGPVLFNNPPITYIAILLVIFSQVLLFHTRWGLRTRAVGEHPRAADTLG
                     VDVFKIRYWNVIIGGVMAGLAGSFLTLEGVGMFERGMTNGRGFIALAVMIFGKWNPIS
                     SALAALFFGFANAMQIQLQFMGIQIPHQFVGMIPYLLTIIVVSGFVGRSRAPAAEGIP
                     YEKE"
     gene            1653..3212
                     /locus_tag="PK791_00925"
     CDS             1653..3212
                     /locus_tag="PK791_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH91910.1"
                     /translation="MDNTYSKTADAVMDTDKSVILEAKHIYKTFPGTVANDDVSLELR
                     KGEVLALLGENGAGKSTLMNVIYGLYTQDKGEIFLNGEPYAIDNPHDAINAGIGMVHQ
                     HFMLVPVFSVLENMMLGAETTKGPFLNKKAAEAKLRDLSESFGLEIDPHAIIEELPVG
                     LQQRVEILKALYRDVEILILDEPTAVLTPQETEELFKIMRRLTERGVSIVFITHKLKE
                     VLAVADRIIIMRRGKVVGTTTPAETDEHGLASMMVGREVILEVEKAPAQVGKVVLEIE
                     HLNIHDNNQMHSVRDISLSVRAGEVLGIAGVQGNGQSEFVEAITGLRRYNSGEIRING
                     KRMPQEKPRTLVEHGMAHIPEDRQKHGLILQYPLTSNFVLCTYYREPFSKHGILNENE
                     ILQNAEELMERFDVRAPSPVTAAGDLSGGNQQKVIVGRELSRDVSLVIANQPTRGLDV
                     GSIEYIHKTLIEMRDAGVGVLLVSSELDEIMSLSDRIAVIYRGEILAVLDAAEASREE
                     IGLLMAGHISE"
     gene            3450..>3979
                     /locus_tag="PK791_00930"
     CDS             3450..>3979
                     /locus_tag="PK791_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH91911.1"
                     /translation="MSKIVVSNISKSFGKTRAVKDISFDVRAGEIFGLLGPNGSGKTT
                     TIRIILDIYQPDSGKVAILGGPMTADKKNHIGYLPEERGLYQDMALERVLTYMASLKG
                     MADADIKTKLPAYLEKFDLAAHAKKKCKELSKGMQQKGQVIATLIHDPELIIIDEPFA
                     ALDPINTQLVKDVLEE"
     assembly_gap    3980..4079
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4080..4513
                     /locus_tag="PK791_00935"
     CDS             <4080..4513
                     /locus_tag="PK791_00935"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HOH91912.1"
                     /translation="AALDPINTQLVKDVLEEERDKGKAIVMCTHQMSQVESLCDRLVL
                     VDQGRVLLTGSLEEIAEHYKTNEVLIKPVSALPASLPGVREISRVNGSYKLSPEEGVT
                     PSQLLETLVSNGVAMESYKVLVPSLDEIFIHVVAEGKKQNG"
     gene            4506..5717
                     /locus_tag="PK791_00940"
     CDS             4506..5717
                     /locus_tag="PK791_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH91913.1"
                     /translation="MAKLFKVAKFEYTRNVFTKRFWVALLLVPLLFILFSLISTFISF
                     KSVDTRPVGLIDRAGIITQAQKLKEKPGLFEIQIEFVPFTDENAAKKASLAGEHQGYI
                     IIPEDYTTSYEIIWKGNKAITEEVMDFATQYLSENLIVNETIPNKARIEEGMNLRLES
                     LDGSIKSAGASWQRIIPPIVISMLYFALVMGSGNYLLQSLVEEKENRTIEIMLTTVSH
                     KQLMAGKIIGNIGVGVTQLLVWAVIIAVALFIFRARIPLLSELIPDPALMALSLVLML
                     VSFVFTASVLAIFGATVTESQEGQQMIGLVVVPILLPVYTLSVFMNSPNGVLARILSF
                     FPFSSPLAMSLRMAFTHVPTLEILLVLVVQVLFAIGAMWLAGRAFELGMLQFNKKVSL
                     FKLFKKEAGDA"
     gene            5710..6960
                     /locus_tag="PK791_00945"
     CDS             5710..6960
                     /locus_tag="PK791_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH91914.1"
                     /translation="MRKIWRVFKYEFMSVVSRRSFILGLILVPLLPTIIIGLVSLLKP
                     KDSPDLVKTIVKEVSTTKPYGVVDESGLINDYPNWAMGQLIPFEDESLARQETQAGNL
                     DGFYVVSADYLETGDVLLVKPEVSMVAEMAYNATFEELIRYNLVDGNDELYAKLVNHV
                     KVTTEYLDIEQADTRDQKDPSTFMVPFFISFFFYMLVIINSSLMLDSVTKEKENRTME
                     ILLSSAEPVDIFGGKILARGLASLLQTVVWLATILLLAKLSNLTLHLPFKLEIPTAVF
                     TWGIPYFLLGFLLYGSLMAGLGAMVPNTREGGQFSLIMNMPLILAYLALSEFVTNPNS
                     AVTLFLSLFPLTSTIVMPTRLVIGAVPIWQLIVALLLLVLAVWLVVRGAANLFSSQNL
                     LSGQKFNLKTFFSTLLFGKKSQKA"
     gene            7066..8223
                     /locus_tag="PK791_00950"
     CDS             7066..8223
                     /locus_tag="PK791_00950"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOH91915.1"
                     /translation="MKQFKTISLFIVVVAMILSACKPNSSDASPTDQLDDIRTVAART
                     IEALTTQMLQTDYARQTETRFSGNETPTPPSEVSPATDTAVPSASFDTTPVPTDAVPT
                     VEKPCDLAFFEGETIPDGSELYPGTVFQKTWTLRNEGSCTWTKDYSVVFISGDSMSAP
                     AVMPLTEKDVKPGESLVITMPLIAPTLGGEYRAHFKLRNAAGVIFAFRNPDHSFWADI
                     KVVEGKIHLVHAYCGAHWTSQAGALHCPGQPGDAGGYVYSDNAPVLETGGIDDEPTLW
                     LGLGAGQDSYIMGVYPAMKIPEKAQFSTVLGCGYGLSRCHVKFTLAYQEGEGQMYELG
                     SWEEHYDEQFTKIALDLSALGGRTVRIILIARAMGSAEDGKIHLLEPVIKP"
     gene            8298..>8572
                     /locus_tag="PK791_00955"
     CDS             8298..>8572
                     /locus_tag="PK791_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91916.1"
                     /translation="MKNIKRYLMILIGLTLLVGLGACGKSNKTDMNSAMSTAVQETIT
                     MGETRTAIARPSDTPIPSPTMMPTIPPIEPTSPQPTFDMTAIYGITQS"
BASE COUNT         2129 a         1785 c         2210 g         2348 t
ORIGIN      
        1 caactatgtg gcaatcaacc ttagcgacta tttttctgaa gggccatgga aagacagaag
       61 tccgggtaac atcgtttcgc gattaccagc tattcagcca tcagcagaga tccccaacat
      121 tggaccagtg cctgttgggt ttatcgtagc ggtattaaca gcgttattga tttggtttgt
      181 gatcaatcgc actacatttg gctttgaaat caattcggtt ggtttgaacc cacatgcttc
      241 aaagtatgcc ggtatgcgca cgaaactgct tattgtagcg gcaatggtgg ttagtggcgc
      301 gttggcgggc gtgggtggcg cggtggaaac aatgggtgtg gtaggtcgtt tccagccagg
      361 ctttaatacc ggtcttggtt ttgatggtat tactgtcgcg cttttggccc gcaataatcc
      421 gattgcagtt attctttcgt cgctcttttt aggcgcgatg aaaggtggcg cgaacacgat
      481 gcagttttac gcgaatgttc ctaatcagat tatcgacatc attcagggca ccatgttact
      541 ttttgtgacg gctgaaatgg tggttcgatg ggtctttaaa ctacgtaaac aacgagatac
      601 cgaaatcacg gttacctcag gatgggggca aagatgatta gcaaaacagt cgataagaag
      661 cgtgagcttt ccattaattg gaaattgttg cgtcaggtca ttctaggctt ggttattgtg
      721 gccgcgattt tgtttttcaa cgcccgctct ccggaaaaaa catccgctgt gttaagcagt
      781 actttacgct acgcggtgcc actcatcctg ggcgccttgt gtggtatttt ctgtgaacgg
      841 gcaggaattg tgaatattgg tattgaaggg cagatgttat tctctgcttt tctttcatat
      901 atgaccaacg tctggaccgg taatttgctc ttgtccttgc tggtgggcat gttgaccggc
      961 gcgatcttag gcctgttttt agcttttctt tcaataaagc ttaagctgaa ccaaaccatt
     1021 ggcggtacgg ttatcaatat tttagcaaca ggtatcacgg gctatttcta tcaaactggc
     1081 ttaacaacgc aaggcaaact ttggaatatt cccctgggac cattggctaa aattccgtta
     1141 atcggaccag ttttgttcaa taatcctccc attacctata tcgcgatatt gcttgtcatt
     1201 ttctcgcagg tgcttttgtt ccatacccga tggggtttgc gcacccgcgc ggttggggaa
     1261 catccccggg cggctgacac cttgggtgta gatgtcttca aaatccgtta ttggaatgtg
     1321 attattggtg gcgtcatggc aggtttggct ggatcatttt tgaccctgga aggggttggc
     1381 atgtttgaac gtggcatgac caacggccgt ggtttcatcg ctttggcagt aatgattttt
     1441 ggaaaatgga acccgatttc atccgcgctg gcagccttgt tctttggttt cgctaacgcg
     1501 atgcaaattc agttacagtt catgggaatt caaattccac atcagtttgt gggtatgatt
     1561 ccatatctgc tgaccattat tgtagtttct ggctttgtgg gcaggtcgcg tgcgcccgcg
     1621 gctgaaggca ttccctacga gaaggagtaa gtatggacaa tacatattct aaaacagctg
     1681 atgccgttat ggatactgat aaaagcgtta tcttagaagc aaaacatatt tacaagactt
     1741 ttccgggtac ggtcgccaat gacgatgtga gccttgaact gcgcaaaggc gaggtactgg
     1801 cattactggg tgaaaatggc gcgggaaaat cgaccctgat gaacgtcatc tatgggcttt
     1861 acacccagga taaaggcgaa attttcctga atggcgaacc ttatgctatc gacaatccgc
     1921 atgatgcgat taatgctgga atcggcatgg ttcaccaaca ctttatgctg gtgccggttt
     1981 tctcggtttt ggaaaacatg atgctgggcg cggaaaccac aaaggggccc tttttgaata
     2041 aaaaggcagc cgaggctaaa ctgcgcgatt tatctgaaag ctttgggctt gaaattgacc
     2101 cacatgctat tatcgaagaa ttgccggttg gccttcaaca acgcgttgag attctcaagg
     2161 cgctttatcg cgacgttgag attttgattt tggatgaacc aaccgctgta ctgaccccac
     2221 aggaaaccga agagcttttt aagattatgc gccgtctgac cgagcgcggt gtttccatcg
     2281 tctttatcac gcataaactg aaagaagttt tggcggttgc tgaccgcatt atcattatgc
     2341 gccgcggcaa agtggtgggc accactacac ctgctgaaac tgacgagcat ggactggcaa
     2401 gcatgatggt cggacgcgaa gtgattttgg aagttgaaaa agcgcccgcg caagtcggaa
     2461 aagtggttct ggaaattgag cacttaaaca ttcatgataa caatcagatg cacagcgtgc
     2521 gtgacatttc actaagtgtg cgtgccggtg aggtgctggg cattgcgggt gttcaaggta
     2581 acggacagag tgagtttgtg gaagccatta ccggtttgcg tcgctataac agtggcgaga
     2641 ttcgcattaa tggcaaacgt atgccccagg agaaaccacg aacgctggtt gaacacggta
     2701 tggcgcatat tccggaagac cggcaaaaac acgggcttat tttgcaatat cctttgacat
     2761 ccaactttgt gctttgcacg tattatcgcg agccattttc aaagcatggc atcttaaacg
     2821 aaaatgagat tttgcaaaat gctgaagaac tcatggagcg tttcgacgtg cgcgcgccat
     2881 cgcctgtgac cgccgctggt gatttgagcg gtggtaatca gcaaaaggtg attgtagggc
     2941 gggaacttag ccgtgatgtg tcgctggtaa ttgccaacca acccacgcgc ggtttggacg
     3001 tgggctcgat tgagtatatc cacaagacgc tgattgaaat gcgcgatgct ggcgttggtg
     3061 ttttgctggt gtcttcggaa cttgatgaaa tcatgtcgct cagtgaccgc atcgcggtga
     3121 tttaccgtgg cgaaattctg gcagtcttgg atgctgctga agcgagtcgc gaagaaatcg
     3181 gcttgctgat ggctgggcat atttcagaat aagcaagcga atgagataag atacaggcac
     3241 tcgaaagggt gcctgaattt tttactggga gatgacgatt ccaggtaatc ggatggagat
     3301 tagaatccat aaggtaaaca agcagaaact ggcgtaaaat aactttgttt tacatgattt
     3361 aggttttttg tttaattaat gcaaaaactt tacaatttct tattctttag acaatgaaaa
     3421 aggggtatga tgtaaagtaa ggagaaaata tgtctaaaat agtagtttct aatatttcta
     3481 aaagttttgg caaaacccgt gccgtaaagg acatttcatt tgacgtgcgc gccggtgaaa
     3541 tttttggtct tttgggtcca aacgggtctg gcaaaacgac cactatccgc atcattctgg
     3601 acatatatca gcctgatagt ggcaaagtgg ctattttggg cggcccgatg accgccgata
     3661 agaaaaatca tattggttat ttgcctgagg aacgtggatt gtatcaggat atggcgctgg
     3721 aacgggtgct aacctatatg gcgagtttaa aaggtatggc ggatgcggac atcaaaacaa
     3781 aattgcctgc ctatttagag aaatttgatc tcgccgcgca tgccaagaag aagtgcaaag
     3841 agttaagtaa aggtatgcaa caaaaagggc aggttatcgc gaccttgatt catgaccctg
     3901 aactgattat tatcgacgag cctttcgccg cgttagaccc catcaacacg caactggtga
     3961 aagatgtgct tgaagaagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     4081 cgccgcgtta gaccccatca acacgcaact ggtgaaagat gtgcttgaag aagaacgcga
     4141 caagggcaag gcgattgtga tgtgtacgca tcagatgagc caggtggagt ccttgtgcga
     4201 ccgtctcgtt ttggttgacc agggtcgggt tttgctaacc ggcagtctgg aagaaattgc
     4261 tgagcactat aaaaccaacg aagttttgat taaacccgtt agcgccttgc ctgcgagttt
     4321 gccgggcgtg cgtgagatta gccgggtgaa tggcagttat aagcttagcc cggaagaagg
     4381 cgtgaccccc agtcagttgc tggaaacgct ggtgagcaat ggcgtggcga tggaaagtta
     4441 caaggttttg gttccatctc tggatgaaat atttattcat gtggtggccg aaggaaaaaa
     4501 gcaaaatggc taagcttttc aaggtcgcca aatttgagta cacgcgcaat gtctttacca
     4561 aacgtttttg ggtggcgttg ttgctggtac cgttgctgtt tatccttttt agtttgattt
     4621 cgacttttat ctcgtttaag tcggtggata cgcgtccggt tgggcttatt gacagggctg
     4681 gaatcatcac gcaagcccaa aagctaaagg aaaaaccggg gttatttgaa attcagattg
     4741 agtttgttcc atttacagac gaaaacgcag ctaaaaaagc cagtttggcg ggggaacatc
     4801 aaggttatat cattatcccg gaagattaca ctacaagtta tgagataatc tggaagggaa
     4861 acaaggcgat tacggaagag gttatggatt tcgcgacgca atatctcagc gagaacttaa
     4921 ttgtcaacga aactatccct aataaagcgc gcattgaaga aggaatgaat ctgcgactcg
     4981 aaagtctgga tggttccatt aaatcagcgg gtgcatcgtg gcaacgcatc attcctccaa
     5041 tcgttatcag catgctgtat tttgcccttg tgatgggcag tggcaactat cttttgcagt
     5101 ccctggtgga agaaaaagaa aaccgcacaa ttgaaattat gctcaccaca gtttctcaca
     5161 agcagttgat ggctggtaag attatcggca atattggggt gggtgttaca cagttgcttg
     5221 tgtgggcggt catcattgct gtggcactct ttattttccg ggcgcgcata ccgcttttga
     5281 gtgagctgat tcctgatcct gctttgatgg cattgagcct ggtcttaatg ttggtttcgt
     5341 ttgtgtttac cgcctcagta ctggctatat ttggcgccac ggtgaccgaa tcgcaggaag
     5401 gacaacagat gattggtttg gtagtcgttc cgatcctttt gcccgtttat acattaagcg
     5461 tctttatgaa ttcaccaaac ggtgtcctgg cgcggatttt gagttttttc cctttcagtt
     5521 cacccctcgc catgagcctg cggatggcat ttacgcacgt gcccacgctt gaaattttgc
     5581 ttgtactcgt tgttcaggta ttgttcgcga ttggtgcgat gtggctggca gggcgcgcgt
     5641 ttgagttggg catgctccag tttaacaaaa aagtttcgct tttcaaattg ttcaaaaagg
     5701 aggctggcga tgcgtaagat ttggcgtgtt tttaaatatg aatttatgag cgtggtcagt
     5761 cgccgttcgt ttatcctggg tttgatttta gtgcctttgc ttccaacgat cattatcggt
     5821 ttggtaagct tgttgaaacc caaggactca cccgatttag tgaaaacgat tgttaaagaa
     5881 gtcagcacta ctaaacctta tggtgttgtt gatgaaagtg gcttgattaa tgactatcca
     5941 aattgggcca tggggcagct gatacccttt gaagatgaaa gtcttgccag gcaagaaacc
     6001 caggctggca atttggacgg tttctacgtc gtttcggcgg attatctcga aacgggtgat
     6061 gttttgctgg tgaagcctga agttagcatg gtcgcggaga tggcatataa cgcgacgttc
     6121 gaggagctaa ttcgctacaa cctggtggat ggtaacgatg aattgtatgc caagctggtc
     6181 aaccatgtta aagtaacgac ggaatatctg gatattgaac aagctgatac ccgtgaccaa
     6241 aaagatccca gtacttttat ggtgccattc ttcatttcgt ttttcttcta catgttggtt
     6301 atcatcaatt caagtttgat gttggattcg gtgacaaaag agaaggaaaa tcgtaccatg
     6361 gagattcttc tttcgtcggc agaacccgtg gatatcttcg ggggtaagat tctggcgcgc
     6421 ggtttggcgt cgcttttgca aactgtggtt tggctggcta cgattttgct gttggcaaag
     6481 ttgagcaatc ttaccttgca tctgccattc aagctggaaa ttccaacagc ggtatttacc
     6541 tggggtatcc cttacttctt gttgggcttt ttgttgtatg gaagtctgat ggcgggtttg
     6601 ggcgcgatgg tgcctaatac gcgtgaggga gggcagtttt ctcttatcat gaatatgccc
     6661 ctgatattgg cgtatcttgc attgagtgaa ttcgttacta acccgaatag tgcggtaacc
     6721 ctgtttctta gcctgttccc gctcacatcc acgattgtaa tgcccacgcg tctggtgatt
     6781 ggcgccgtac cgatttggca gctgatcgtc gcgcttttgt tgctggtgct ggcagtttgg
     6841 cttgttgtgc ggggtgctgc caacttgttc agttcccaaa acctgctttc aggtcaaaaa
     6901 tttaacctga agaccttttt ctctacgtta ctgttcggga agaaatccca aaaagcatag
     6961 ccttttctgg attgtaactt gcaactgaat aagcatagga ctctatcaga atcctatgct
     7021 tgtttgcgtt aagtgaatgt gaaaagacgg tgtttcggag gatctatgaa acaatttaag
     7081 acaatcagcc tgtttattgt cgtcgttgcg atgatattga gcgcctgtaa accgaacagt
     7141 tcagatgcga gcccaacaga tcagctggac gatattcgca cggtggcagc gcgcacgatt
     7201 gaagcactca ccacgcaaat gctgcaaacc gattatgcca ggcagaccga aacgagattc
     7261 tcaggcaatg aaacgccaac gccaccaagc gaagtatcac ccgcgacgga cacagcggtt
     7321 ccaagcgcca gttttgatac tacacctgta ccgactgacg ctgtgccaac tgtagaaaaa
     7381 ccatgcgacc tggctttttt tgaaggtgaa acgattcccg atgggagtga actttatccc
     7441 ggcaccgtgt tccaaaaaac ctggactttg cgcaatgaag gaagctgtac ctggaccaaa
     7501 gattactcgg tggtctttat cagtggcgat tcaatgtccg cgccagccgt gatgcctttg
     7561 accgaaaaag acgtcaaacc cggcgaaagc cttgtgataa ccatgcccct gatcgcgcca
     7621 actttgggcg gcgagtatcg cgctcatttt aagttgcgca acgcggcagg ggtaatattc
     7681 gcgtttagaa atcccgacca tagcttttgg gcggatatca aggtggtgga aggtaaaatt
     7741 cacctggttc acgcttattg tggcgcccat tggacaagtc aggcaggtgc tttacattgt
     7801 cccggtcagc caggtgacgc tggtggatat gtctattccg ataacgcgcc tgtgctggaa
     7861 accggtggca ttgacgatga gccaacgctt tggctgggcc tgggtgcggg gcaagatagc
     7921 tacatcatgg gcgtttatcc cgcgatgaag atccccgaaa aagcgcagtt cagcactgtt
     7981 ttgggctgtg gatatggctt gagccgctgc catgtgaagt ttaccctggc atatcaggaa
     8041 ggcgaagggc aaatgtatga acttgggtcc tgggaagaac actacgacga acagtttaca
     8101 aagattgcac tcgatttgag cgcgttaggc ggaaggacag taaggatcat attaatcgcg
     8161 cgcgcgatgg gttccgcaga agatggtaaa attcatttat tagagcctgt aatcaaaccc
     8221 tgattcttta ccggacctgg ctttttaaaa accttgtaac gaggatttgc catttgtaaa
     8281 ctggcattgg agtaatgatg aaaaacataa aacgctatct gatgattctt atcggtctga
     8341 cgcttctcgt tggacttggg gcgtgtggaa aatcaaataa gactgacatg aattcggcca
     8401 tgtcgaccgc ggtgcaggaa acaataacca tgggcgaaac ccgcacagct atcgcgcgac
     8461 cgagtgacac acccataccc agtccgacca tgatgcctac cattccaccg attgagccaa
     8521 cttcaccaca acccactttt gatatgaccg ccatctatgg cattacgcaa tc
//
LOCUS       DAOSFC010000017         8441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7181_length_8441_cov_4.714524, whole genome shotgun sequence.
ACCESSION   DAOSFC010000017 DAOSFC010000000
VERSION     DAOSFC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7181_length_8441_cov_4.714524"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1614)
                     /locus_tag="PK791_00960"
     CDS             complement(220..1614)
                     /locus_tag="PK791_00960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH91917.1"
                     /translation="MSKKPSSPTLTTKKHVVRQEREDRQIKIAVTVTSIVLGVVLLIV
                     LFALVNTFIIKPNTVIATVGDTEIRAGDFDKSVRYRRFNMLNQASQYYNNYAQFQSFA
                     PDYAHQFLAVAQRNAVELNQGDIIGNQVLNDMIDDILIAEEAEKLGISVSKEELNAYI
                     EENFGYFENGTPTPANTATPFNTPTISSQQETLIALPTEELPVEEDNEDALVPSDEAE
                     TPVDAEVTEEPVEETEVVEEEQTEPTEEATATLVPTITPSPTPYTRRLFEKEYRSFRS
                     QMDKIDISRSQLERILYNALLRTKVMEAVTADVPAEEEQVWARHILVASLEEAEAIIE
                     RLNAGEDFAALAMELSTDEGSKINGGDLGWFGKGRMVPEFQEAAYALENIGDISEPVN
                     STHGWHIIQLLGKGVNPVDERTHETLKATFFEDWIKELRAQRDDIVIKDKWAEFVPMK
                     PAISPSFYNAIMNP"
     gene            complement(1629..1859)
                     /gene="rpsR"
                     /locus_tag="PK791_00965"
                     /pseudo
     CDS             complement(1629..1859)
                     /gene="rpsR"
                     /locus_tag="PK791_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004648825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(1983..2264)
                     /gene="rpsF"
                     /locus_tag="PK791_00970"
     CDS             complement(1983..2264)
                     /gene="rpsF"
                     /locus_tag="PK791_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOH91918.1"
                     /translation="MHKYETVLVFQADLEEVALNALVESVETLINSVGELTKIDRWGK
                     RKLAYPIRKQFEGYYVYMTFDGEPSEIANIKRTLGYNEQILRYIIVRLD"
     gene            complement(2447..2836)
                     /locus_tag="PK791_00975"
     CDS             complement(2447..2836)
                     /locus_tag="PK791_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOH91919.1"
                     /translation="MKFLKRVLVIAIALFIAGLVMSNMSGFEITKNGWVQYAVMAVIL
                     AILNAVLLPILKTLSCGAIILTLGFFSLILNALVFWLASVISTNLLGGGITITKFWPA
                     FVGSLIVSLATSIFVRDDNKSTSGNDD"
     gene            complement(2886..3125)
                     /locus_tag="PK791_00980"
     CDS             complement(2886..3125)
                     /locus_tag="PK791_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91920.1"
                     /translation="MENINLPPCFEPIRRDMIDYYLIMHTVTRISSFGGHAYTVPLLE
                     EAVMLQNRVDQKMEEIDKCLPDCPTSFVESFQARE"
     gene            complement(3512..3584)
                     /locus_tag="PK791_00985"
     tRNA            complement(3512..3584)
                     /locus_tag="PK791_00985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3549..3551),aa:Arg,seq:tct)
     gene            complement(3617..4042)
                     /locus_tag="PK791_00990"
     CDS             complement(3617..4042)
                     /locus_tag="PK791_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91921.1"
                     /translation="MLNKLPSLIKPTLTTNFHIDFDWWKKQDRNWRNALESFLCEEHQ
                     PFFSSAEGITDADLIDPETGEVSQGDPLIDILTNHCAKQEGFLSQSGPLVDSIFKVFL
                     VNNNQPLNSKELAEITGRDADIILRTIGTLRVYKGIRPV"
     gene            complement(4050..5636)
                     /locus_tag="PK791_00995"
     CDS             complement(4050..5636)
                     /locus_tag="PK791_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91922.1"
                     /translation="MAWQTKNNFADYWKNLTINQNDLNALTNHLFETESPAVIEVLAT
                     VFVNNRLKEIQAEEELKQEEAGFFYHPKEEYPVGTKIIFPELNWISGVVAATREGNNP
                     IFGDFSVITVEFDDGSTKEFASKLEDHILNDKTYMSDTISEIDANSIMQEFGVQIKRQ
                     LSQALANQNELVLIGHSWFPKSLLIDIGIGSLNIAEAILDSMDGGPIGIAELMKQLEM
                     TDEENKQLLEFSLNYALQEDSRFDEVGSSGVFSWYLKRMEPKSVLEVPLYLQSDCQSL
                     EREGFDPGLEKLFSELSDELTWQIDPHEVYEPVKKVRIPLSYPHWRAGTLPITPETYT
                     IFPSALQSERIKIDFQDEISKERIPAWVVRPHRYVTGLKEWYEDQNLIPGSIVEIAST
                     KDPGVIKINIERKRSNKEWIKTVLVGADGGIVFALLRQSIYAGFNDRMAIAIPDVKGL
                     DQIWENRSKKEIPLKNDVTRLMHELSKLNNQSHVHFVDLYAGINVIRRVSPQELFFTL
                     ADNDEFTHIGDNYYHLSASQ"
     gene            complement(5773..5967)
                     /locus_tag="PK791_01000"
     CDS             complement(5773..5967)
                     /locus_tag="PK791_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91923.1"
                     /translation="MTVAENNENELKKLITERFFSIFESHSSELSKEEKIAFGKTLAL
                     NLISIASIGLVIGFLISFLI"
     gene            complement(5969..6190)
                     /locus_tag="PK791_01005"
     CDS             complement(5969..6190)
                     /locus_tag="PK791_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91924.1"
                     /translation="MDNVTIQKVINTIYSEYPKTRGGSPRISRQANGRNLLVFSFFDT
                     LPGSKSIQQNLRVVVDDQGRILKKSISRG"
     gene            complement(6202..7395)
                     /gene="ftsZ"
                     /locus_tag="PK791_01010"
     CDS             complement(6202..7395)
                     /gene="ftsZ"
                     /locus_tag="PK791_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOH91925.1"
                     /translation="MNISSAQTNPIGKPSLPKIVVLGLGGGGSNAINRMIENGMHDVV
                     FIAANTDAQALAKSLAPIKIQLGPKSTRGLGAGGQPIVGEEAAEESTRELAEHLRGAD
                     MVFLTAGMGGGTGTGAISIAAKVAKASGAVTVSIVTKPFSFELGRRQKNSEEGIEKLK
                     EFSDTLIVIPNDKLIEIAPRDLPLEMAFTLADDILRQGVQGISELITETGVINVDFAH
                     IRNVMTRGGGALIAVGHGSGPDKVQQAIDKALNHPLLDDIDLYSATAIIANFTGSADM
                     SFKEITEAMQRLQEATGNNAEIIPGIITDSRMGDSIQVILIVTGISENNPVNIPSLTK
                     TNQSQARQEFQDFKFDLDEPDEYAHTQAKQDANNQELEIDPISQSIDLSNDLSIPAFL
                     RRRNK"
     gene            complement(7412..>8441)
                     /locus_tag="PK791_01015"
     CDS             complement(7412..>8441)
                     /locus_tag="PK791_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91926.1"
                     /translation="AKWGVKYVIFFDKPNTKKAWMPGTWSQGDLVERFLDRYLSFVRL
                     AEQNGITPVFPALEPGGDYWDLSFLRKAFQLAGQRRSIEFGANLHIAVSGQSFSNPIN
                     WGDGGEKKWRVPRPYAKTELGEQDHFGINTYQWYAQIAKSTLNTTPKFIMLYMGTSDV
                     ISKTMDENLDYEQLLDLVTFDTSNDNDTFSINKSVIACLFWLINTPKKRTKQDTTPTT
                     LFETQEQTKPEWIEDLQAKVEETQKQSTELKLANGLADWVHAIDHYLLLPSYEWGIPV
                     NTLDKVRPIIAESRPTIGFSIAEACNARKVTVWNENGAFSEKDIQTLRESGCIIDEHI
                     INSLSISA"
BASE COUNT         2355 a         1658 c         1803 g         2625 t
ORIGIN      
        1 taacccataa cagaaatacc gttgcagctg ctgatgtgat ctatggcgta accatgggga
       61 aagattcagc cagtcaatta atcagcttaa ggctcgatga agtcgatagc agttacatga
      121 attcataaaa ccaaaagtaa taagtgataa aaaagactgc cagattcatt taggcagtct
      181 tttttttaat atattattct tgcgatatat taatcaccgt taggggttca tgattgcatt
      241 atagaaactc ggtgaaattg ctggcttcat aggaacaaat tcagcccatt tgtctttgat
      301 gacaatgtca tctcgttgag cgcggagttc ttttatccaa tcttcaaaga aggtcgcttt
      361 tagtgtttca tgggtgcgtt cgtccacagg atttacgcct ttgccaagca gttgaataat
      421 gtgccagcca tgcgttgaat taacgggttc gctgatatcg ccaatgttct caagggcgta
      481 agctgcttct tgaaattcgg gaaccattcg gcctttgcca aaccaaccca aatcaccacc
      541 gttgattttc gaaccttcgt cggttgagag ttccattgct aaagcagcaa agtcttcgcc
      601 agcatttaag cgttcaataa tggcttcagc ttcttctaat gaagcaacca aaatatgacg
      661 tgcccaaacc tgttcttctt cagcaggaac atctgcggtt acggcttcca ttacttttgt
      721 gcgtagaagg gcgttgtata gaatgcgttc aagttgcgaa cgactgatat cgatcttatc
      781 catctgactt cggaagcttc gatattcttt ttcgaagaga cgtcttgtat agggtgtagg
      841 tgaaggggtg atagtgggaa caagggttgc agttgcttct tcggtaggtt ctgtttgttc
      901 ttcttcaaca acttcagttt cttcaacagg ttcttccgta acttcagcgt ctacaggagt
      961 ttcagcttca tcagaaggca ctaaggcatc ttcattgtct tcttcaacag gaagttcttc
     1021 agtaggaagt gcaatcaggg tttcctgctg actggatatg gtaggtgtat tgaagggtgt
     1081 tgctgtatta gcaggtgttg gagtgccatt ttcgaaataa ccaaagtttt cttcgatata
     1141 ggcgttaagt tcttctttgc taacactaat gcctagtttt tctgcttctt cagcgatgag
     1201 aatatcatca atcatgtcgt tgagaacttg attgccgatg atatcgccct ggttaagttc
     1261 tacagcgttg cgttgcgcta cggctaaaaa ctgatgtgcg taatcgggag cgaagctttg
     1321 gaattgtgca taattgttgt aatactggct tgcctgatta agcatattaa atcgcctgta
     1381 gcgaacactt ttgtcaaagt caccagctct gatttccgta tcgcctacag tggcgatgac
     1441 ggtgttgggc ttgataataa aagtgttaac taaagcgaaa agtacaatca gtaaaacgac
     1501 gccaagtaca atgcttgtaa ctgttactgc aattttaatt tgtcgatctt cgcgttcctg
     1561 acgaacaaca tgcttctttg ttgtcagagt tggggagctg ggtttcttgg acataatcca
     1621 tcaccgattt agtactcagc ccaaaagtcg ccctcaaagg ggattaatgc gatgtggcga
     1681 gcattcttga ttgcggtcgc aacttcgcgc tgatgcttgg cgcaagttcc ggtttgtcga
     1741 cggggtctga ttttacctgt ttcgttgatg tagcgaccca ttaactcgat gtttttataa
     1801 tcgattttaa gattcttgtc tgcacagaat tgacaaaact tcggacggga aacgaaacga
     1861 cgtcgctcac ttcgttgctg tcggtcttgg cgttctggac gatcggagcg ttctgatcgc
     1921 tctgtttttt caacttgttc ttgctttttt tctgatgtat tgtctgtcat aattactcct
     1981 aattaatcca aacgtacaat gatgtaacgc aaaatttgct cattgtaacc aagggttcgt
     2041 ttgatatttg cgatttctga aggctcacca tcaaaagtca tgtagacata gtaaccttca
     2101 aattgtttgc gaatggggta ggcgagtttc cgttttcccc aacggtcaat tttcgtgagt
     2161 tcaccaacgc tattgatgag tgtctctacg ctttctacga gggcgtttaa cgccacttct
     2221 tccaggtctg cctggaaaac caatacagtt tcatatttat gcatgcgctc gaggctcctt
     2281 tcctcgggtt tgctcttgct tgtggccttt ggcttaacaa gagcggaagg ttgagattca
     2341 ggatttattc ctgtttaaca ggcgtaagtc taacataaat aataaaaaaa gcaagcctga
     2401 taatttgaaa ggacttgctt ggtgggtttg aaagaagatt tagatattag tcgtcattcc
     2461 cggacgttga tttattgtcg tcacgaacaa agatagatgt tgcaagactg acaataagtg
     2521 aaccaacaaa tgccggccaa aatttggtga tggtaatgcc accgccaaga agattagtgg
     2581 atatgacaga tgctaaccaa aaaaccaagg catttaaaat aagactaaaa aagcctaaag
     2641 ttaagatgat tgcaccacag gataatgttt ttaatatggg tagaagcacg gcatttaata
     2701 tggcaaggat tactgccata actgcatact gaacccaacc gtttttggtg atttcaaaac
     2761 cagacatatt tgacatgaca agcccagcga taaacaaggc gattgcaatg acgagaactc
     2821 tttttaaaaa tttcatttat ctcctatcgt gacttatata ttaaaacaaa gaacgatatt
     2881 gaaagttact ctcttgcttg aaaactttca acaaaagatg tgggacaatc aggcaagcat
     2941 ttgtcaattt cttccatttt ttggtcaaca cggttttgaa gcataacagc ttcttccaat
     3001 aaaggaacag tgtaagcgtg tccgccaaaa gagctaatgc gtgtaacggt gtgcatgatg
     3061 agatagtaat caatcatatc ccgacgaata ggctcaaaac atgggggtaa gtttatattt
     3121 tccatgaaat atcgttcctg catgataatg gatgggcctg aaatagggat atagccatgg
     3181 tcctgaattc tcataacgcg atcacgtgaa tcatgaagca ttttttgaac ccgtagatag
     3241 ttgattcgtg agcatgatgt atctgtaatc ggcatgctta tataatgatt aaatactgcc
     3301 aaaacaacca ttacaaagac gataagaata agagcaattc gtctttcgga gaaaggcgtt
     3361 actttagggt cataaggaaa tttggttcca caccgttcgc atattcttgc gccattgtcg
     3421 tatagagtgc tacaatcggt acattttctc atggttcgat cctatcatca tatttgttag
     3481 gataactaaa aggtttccgt attaaagagt gcacccccgc cgcgactcga acgcggaacc
     3541 tacggtttag aagaccggcg ctctatccat tgagctacgg gggcttattg gtttaacaga
     3601 cgaattataa cattttttag acagggcgta tacccttgta aactcttaat gttccaattg
     3661 ttctaaggat aatgtcagca tcacgtccgg taatttctgc tagttctttg gaattgagtg
     3721 gctgattgtt attgactaag aatactttaa agatactatc gactaaaggt ccactttgac
     3781 tcaaaaagcc ttcttgttta gcgcaatggt tggtaagaat atcaatgagt ggatcgcctt
     3841 gacttacttc gccagtttct ggatcaatga gatcagcgtc tgttatgcct tcagcactgc
     3901 taaaaaaagg ctggtgttct tcgcaaagaa agctttcaag tgcatttcgc cagttgcggt
     3961 cttgtttttt ccaccaatcg aagtcaatat gaaagtttgt tgtgagggta ggttttatca
     4021 aactgggcaa tttatttaac atagtatcct cattgtgaag cgcttaagtg gtagtagtta
     4081 tcacctatat gtgtgaattc atcgttatcg gccagggtaa agaataactc ctggggagag
     4141 acacgtctga taacattgat tccagcatat aggtcaacaa agtgaacatg ggactgattg
     4201 tttaatttag atagctcgtg cattaagcga gtgacatcat ttttaagtgg tatttctttc
     4261 ttgctgcgat tttcccagat ctggtcaaga cctttaacgt ctgggatagc gattgccata
     4321 cggtcattaa agccagcata aatagattgt cgcaaaaggg cgaagacgat gccaccatca
     4381 gcaccaacga gaacggtttt aatccactct ttgttggagc gtttacgttc aatattaatt
     4441 tttataacac cagggtcttt tgtactggcg atttcaacaa tgcttcctgg gattaaattc
     4501 tggtcttcgt accattcttt cagaccggtt acatatctgt gagggcgtac aacccaagct
     4561 ggaatacgct ctttagaaat ttcatcttgg aaatcgattt tgatccgctc gctttgtaat
     4621 gcgctaggaa aaattgtata ggtttcaggt gtaatgggca aggtgccggc acgccaatgg
     4681 ggataactaa ggggaatgcg cactttttta acaggctcgt aaacttcatg agggtctatt
     4741 tgccaggtta gttcatcaga aagttcagag aataattttt ctaagccagg atcaaaacct
     4801 tcacgttcaa gtgactgaca gtcactttga agataaaggg gtacttcgag aacagatttt
     4861 ggctccatac gtttgagata ccatgaaaat acaccacttg aaccgacttc gtcaaaacgt
     4921 gaatcttctt gcaatgcgta attgagcgaa aactctaaca actgtttatt ctcttcatca
     4981 gtcatttcca gttgtttcat tagttcagca atgccaattg ggccaccgtc catagaatcg
     5041 agaatggctt cagcaatatt taaggagccg ataccaatat caattaataa ggatttgggg
     5101 aaccaggaat gtccgattaa aacaagttcg ttttgatttg ctaaggcttg tgaaagctga
     5161 cgtttaatct gcactccgaa ttcctgcata atggaattag catctatctc gctgatggta
     5221 tcgctcatat aggttttatc gtttaatatg tggtcttcca gtttagatgc aaattctttt
     5281 gtactgccat catcaaattc aactgttatt actgaaaaat caccaaagat gggattattt
     5341 ccttcacgtg tggctgcgac aacaccacta atccaattta gttcggggaa aatgattttc
     5401 gtaccaacag gatattcttc tttagggtga tagaaaaagc cggcttcttc ctgcttgagt
     5461 tcttcttccg cctgaatttc cttaagacga ttgtttacaa ataccgttgc caagacctca
     5521 atgactgcgg gggattctgt ttcaaaaaga tgattagtta acgcattgag atcattctgg
     5581 tttatggtta aatttttcca gtaatctgca aaattgtttt tagtttgcca agccatacat
     5641 acctctgcta agaagaattc tttatgatta tacattaaaa acttgatatt aacgcctaag
     5701 tcagataata aggataaata aaaaatccag cctgcgtaaa acaggctgga atagggagga
     5761 ggatatgaat cattagatga gaaaagaaat caagaatcca ataaccaagc cgatgcttgc
     5821 aatggatata aggttaagtg ccaatgtttt gccaaatgct attttttctt cttttgataa
     5881 ttcagaagaa tgagattcaa atatactaaa gaatctttct gtaataagct ttttcaattc
     5941 attttcattg ttttcagcaa ccgtcatctt aaccccttga aattgacttt ttaaggatgc
     6001 gtccctgatc atcaacgacc acgcgtagat tttgttgaat acttttactg ccagggagcg
     6061 tatcgaagaa tgagaaaacc aatagatttc tcccatttgc ctgacggctt attcttggtg
     6121 aaccgcctct tgtttttgga tattcactat aaatcgtgtt aattactttt tgaattgtga
     6181 cgttatccat aaatactcct gctatttgtt tctgcgacgc aaaaatgctg ggattgacaa
     6241 gtcattggaa aggtcgatag attgagaaat agggtcaatc tcaagttcct gattgttggc
     6301 atcttgtttg gcttgtgtgt gcgcatattc atcaggttca tccaaatcaa atttgaaatc
     6361 ttgaaattct tgcctggctt gtgattgatt tgtttttgta agtgaaggaa tattcactgg
     6421 attgttttct gaaataccgg tgacaataag aataacctgg atactgtctc ccatacgaga
     6481 gtcggtgatg attcctggga taatttcagc attgttacca gttgcttctt gaagacgttg
     6541 catagcttca gtaatttctt tgaatgacat atcagcagaa cctgtgaagt tagcaataat
     6601 agctgttgca gaataaaggt cgatatcatc taacagggga tgattgagag ccttatcgat
     6661 agcttgttga accttgtcgg ggccactgcc atgaccaaca gcaattaggg caccgccgcc
     6721 acgggtcatg acgtttctga tatgtgcgaa gtcaacattg atgacgcctg tttcggtgat
     6781 cagttcggaa ataccctgaa cgccttggcg taagatatca tcagcgagag tgaaggccat
     6841 ttccagaggg agatcgcgtg gtgcgatttc gattaattta tcgttgggta taacaataag
     6901 cgtatcagaa aattctttta gcttttcaat accttcttca gagttctttt gacgacgacc
     6961 taattcaaat gagaagggtt tggtgacgat tgagacagta acggcaccgc tggctttggc
     7021 aactttggct gcgatggaaa tagcgccggt gccagtaccg ccccccatgc cggcagtaag
     7081 gaataccata tcagcaccgc gaaggtgttc tgccagttca cgagtacttt cttcagcagc
     7141 ctcttcacca acgataggct gtccgccagc ccccaaacca cgcgtagatt ttgggcctaa
     7201 ttgaattttg atgggggcta aactttttgc cagtgcctgt gcgtctgtat ttgcagcgat
     7261 aaaaacaacg tcgtgcatgc cattttcaat catgcggtta atcgcatttg agccaccacc
     7321 ccccagaccc aaaacaacaa ttttaggtaa actgggtttt cctatgggat tagtttgtgc
     7381 agatgatata ttcatttcct tctcctgaaa attatgcaga gatgctaaga ctgttgatga
     7441 tatgttcatc aatgatgcat cctgattctc ttaatgtttg tatatccttt tcgctaaaag
     7501 caccattttc gttccaaacg gtaactttac gtgcgttaca tgcttcagca attgaaaagc
     7561 caatcgttgg acggctttca gcaatgatcg gtctaacttt gtctaaggtg tttaccggaa
     7621 taccccattc gtatgatggt aataataggt agtggtcaat tgcatgaacc caatcagcca
     7681 atccattcgc caatttaagc tcagtggatt gcttttgtgt ttcttcaact tttgcttgta
     7741 aatcttcaat ccactctggt ttggtttgtt cctgagtttc aaacagcgta gtcggcgtgg
     7801 tatcttgttt tgtgcgtttt ttaggtgtgt ttatcagcca aaacaggcaa gcaataacgc
     7861 ttttgttaat cgaaaacgtg tcattatcgt tgctggtatc aaaggtaaca aggtccaaaa
     7921 gttgttcata atcaaggttc tcatccattg tttttgaaat aacatcagag gtgcccatat
     7981 aaagcataat aaattttggc gtggtattga gtgttgattt ggcaatttgc gcataccatt
     8041 ggtaggtatt aataccaaaa tggtcttgtt cacccaattc agttttagca taaggtctgg
     8101 gaacccgcca tttcttttca ccaccatcac cccagttgat aggattggaa aaactttggc
     8161 cactaactgc aatatggaga ttagcgccaa actcgattga gcgtctttgg ccggcaagct
     8221 ggaaagcttt gcgtaaaaaa gaaagatccc aataatcgcc acctggttca agagcaggaa
     8281 agacgggtgt tataccattt tgttccgcaa gtctaacaaa tgagagatag cggtccaaaa
     8341 agcgttcaac caagtctcct tgtgaccagg taccaggcat ccaggctttt ttggtattgg
     8401 gtttgtcaaa aaaaatgacg tatttaacgc cccatttcgc a
//
LOCUS       DAOSFC010000018         8437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7188_length_8437_cov_4.679671, whole genome shotgun sequence.
ACCESSION   DAOSFC010000018 DAOSFC010000000
VERSION     DAOSFC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7188_length_8437_cov_4.679671"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PK791_01020"
     CDS             <1..610
                     /locus_tag="PK791_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOH91927.1"
                     /translation="QVTGKKVKALRRQGILPGVIYGHKTEPLAIQMDAHSTGLIMGKL
                     TGTSIVTLDVDGEKHTAIVRDRQRDVIYEELLHVDFLEISLKELLRATVAIELVGEAP
                     VSKLGDVIINHVLTDLDIEALPMDLPERIEVDISGIETVEDTITVADLNLGEKITIHT
                     PEDEVIVTVGFVAAEVEGEEDEMAEGSAEPEVISAREAEDEE"
     gene            complement(700..1317)
                     /locus_tag="PK791_01025"
     CDS             complement(700..1317)
                     /locus_tag="PK791_01025"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOH91928.1"
                     /translation="MTDNLLNACQGLPADLAQRLATHLATNPGTIIDKRLQLTNPLHP
                     AAVLIPLLCHQGEWHILLTRRTEKLWSHPGQVAFPGGAWEESDANLVQTALRESFEEI
                     SLLPESVTVLGTMPTFGTISHFALTPVVGIISWPQELFLRPEEVARAFIVPLSFLKDP
                     ANRYYQDFVHESQSHPVLYFKRYDGELIWGITAQIILQFLEITES"
     gene            complement(1314..2165)
                     /locus_tag="PK791_01030"
     CDS             complement(1314..2165)
                     /locus_tag="PK791_01030"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOH91929.1"
                     /translation="MNSAIFTDSNFAQEGDLVQLQGTGHRSHLLVLEAGAVLQTHRGV
                     IRHDDIIGTAWGTRLESHQGNPFYLLQPSLSDLIKGLKRGTQIMYPKEISYILFHMGI
                     GPGKRVIECGTGSGGLTTALAYMVGTTGKVYSYERKAQIQDLAIKNLTKFGLQNRVDF
                     KVGNAEDGFDEENVDAIILDLPNPQDYLHHVRKSLRGGGSLGMILPTFNQVEIILREL
                     KLANFAMIEVSEILQRFYKTDWARLRPVDRMIGHTGFLIFGRKVDRLAAEDSDDDAFD
                     NEQGNQD"
     gene            complement(2171..2437)
                     /locus_tag="PK791_01035"
     CDS             complement(2171..2437)
                     /locus_tag="PK791_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91930.1"
                     /translation="MNITSYIFGGLLAALFGALFHLWRGGGFWKLILYLALSWLGFYL
                     GHLVFLHYDVKFMTVGGGQMAGGIVGSLVFLFLGNWFTRINPEG"
     gene            2688..3494
                     /locus_tag="PK791_01040"
     CDS             2688..3494
                     /locus_tag="PK791_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOH91931.1"
                     /translation="MFADTHCHLDDRRFQNDLPQVIKRAEDAGLVLIINSGADMDSSE
                     ASVRLAQQYEGFIFANVGLHPNRINELTAQTLKRLRLLSKSPEVLAIGEIGLDYHWNT
                     FPVWQQKEAFRTQLDLAAEMELPVVIHSRKSTDDVVEILLEWHAGLPAEHPQKARPGA
                     LHSWSDTYEKAKPLIEAGFYFGVSGPVTYLNAPERRECTAALPLERILIETDCPYLTP
                     HPLRGKRNEPAYVRKMVEEVARLHGKTIDETAKITTQNAFRLYGLRVQVA"
     gene            complement(3575..4300)
                     /locus_tag="PK791_01045"
     CDS             complement(3575..4300)
                     /locus_tag="PK791_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH91932.1"
                     /translation="MEPSGTKKKILVADDERGLVKVIHLNLEHDGFDVVEAMNGQQAM
                     DQLRTTLPDLVLLDIMMPDMDGFTVLKLIREQSQIPVIMLTAKSEEADIVQGLELGAD
                     DYITKPFSPRELTSRIRAVLRRSAARSEEESGKIVVDDRLTIDFDRHEVWVEGELVQL
                     RPTEYRLLYHLVKNAGWVLTHDQILNKVWGYEYENEPHYVRLYINYLRKKLEKDPSNP
                     KYILTERGTGYRFVDYKREGFSA"
     gene            complement(4311..>4651)
                     /locus_tag="PK791_01050"
     CDS             complement(4311..>4651)
                     /locus_tag="PK791_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91933.1"
                     /translation="KALTLDHSLVQRLVELGQISQEDANDHPQRNVLFRALGQNEGFR
                     ADIGHVEITEPCTLLLCTDGLWGLVDQETLSKRINDGGNYEKMVNSLIEMANNAGGTD
                     NISAILVKIS"
     assembly_gap    4652..4751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4752..5002)
                     /locus_tag="PK791_01055"
     CDS             complement(<4752..5002)
                     /locus_tag="PK791_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91934.1"
                     /translation="MSIFSRRRKNKPIDLANAFSNEQIPVHLEVQLAQSLGMTRTHME
                     DAIYALNIKTQLPKSRSTLGIFMVADGMGGHMYGDIASS"
     gene            complement(5033..5527)
                     /locus_tag="PK791_01060"
     CDS             complement(5033..5527)
                     /locus_tag="PK791_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91935.1"
                     /translation="MTESRNNKIHPLAILGAFIFALVLLGFAYIIFNKSAEKANAPDQ
                     QAVYNITLIAAPTQTATVFVPTQLPTPTQPAPNILPDDTIGVGSFVKVVGTKGLGLNI
                     RNSPGRGQTINFLALDAEMFEVVGGPEVIDDLIWWQLKAPYDANRTGWAAEDYLERVR
                     QDLP"
     gene            5733..7196
                     /locus_tag="PK791_01065"
     CDS             5733..7196
                     /locus_tag="PK791_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH91936.1"
                     /translation="MLAGLFLLAAIVLTVISIVQFKSVAVVYPNGSQIGDIPVGGLSR
                     KMAEERLRDAYSIPVELRYGDARLQFSPDVLGLTLNIKETLDNADEQNPGSTWWAYLW
                     GKLETPIAFTLPMQFGENQAMVRQVLEDTFPNRYDQLASATIPVVNSYHVLPGEPGLA
                     LADYDETVAQIMNALKSPNQRVVQLRVEESPALAMLWENLEIKLRQTIEQEGFNGLVE
                     IYLEDLHDGRLMHFATRGGTDVPVDVAYSGASTVKIPIMVSTMRRVSDPIPATASTWM
                     KAMIQDSLNPPADGLMKNYMDNNTGPLMVTDDMRELGYQNTFMAGFFEPGSPLLYKYE
                     TPANTRTDVYLNPDTYNQTVPSEIGDLLKRIYLCAKHDSPEYNLFDGEVNQSECQIMI
                     DNLLSNRMGALIEVGVAPEGRVAHKHGWTNETDGLLHTISDVGIVFSPGGDYVLVIFI
                     HSTGQLLFDEGNLLFARLSQSIYNAYNPYQQAVVYIE"
     gene            7216..>8437
                     /locus_tag="PK791_01070"
     CDS             7216..>8437
                     /locus_tag="PK791_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH91937.1"
                     /translation="MRLIMTHEQADLDALASMLGAHILMPDAYAVLPRQINRNGQAFL
                     RKFGKELGFTRSHDLPRKNIEEIILVDTQSMVTLRGITRDTNVRVIDHHARKSREQAE
                     WDYEIHDTGACTTVLVEKIMEANLPVSPQEATLMLLGIYEDTGSLTYPNTIARDASAV
                     SYLLTMGADLNIASKYLNPPLSNAQMQLYDRLLQNLMTVHIEQSMLVVACASALDLND
                     EISSVAHRLREFLEPDGLIVLALTRMGLRLVARGNSPDVDMAKLAKHFGGGGHQRAAS
                     ALIRLQSRPVAEQSELVLNHYQQKVLDFLPHIVSPSLQVKHIMSQNPLLLSPETTVQE
                     VAELMNRYGFEGYPVVEDGRVIGLLNRRNVDRALHHKLDATTRELMDSGEVSVVPEDS
                     ISYLQELMTASGWGQ"
BASE COUNT         2055 a         1862 c         2233 g         2187 t
ORIGIN      
        1 acaagtcact ggcaagaagg tgaaagcctt gcgccgacag ggtattttgc ccggtgttat
       61 ttacggtcac aaaactgaac cgctcgctat tcaaatggat gcccacagca ctgggttgat
      121 tatgggtaaa ttgacgggta ccagtattgt tactttggat gttgacggtg aaaaacacac
      181 ggctatcgtg cgtgaccgcc aacgcgacgt catctacgaa gaactcctgc atgttgactt
      241 ccttgagatt tcactcaaag aacttttgcg cgcgaccgtg gcaatcgagt tggttggcga
      301 agcccctgtt tcgaaactgg gcgacgtgat tattaaccat gttttgaccg atctcgatat
      361 tgaagccttg ccgatggacc ttcctgaacg tattgaggtt gacatcagtg gcatcgagac
      421 tgttgaagat acgattaccg ttgctgacct gaatctcggc gaaaagatta ccatccatac
      481 tcccgaagac gaagttatcg taactgttgg ctttgtggct gctgaagttg agggtgaaga
      541 agatgaaatg gctgaaggtt ccgcagaacc tgaggttatc tccgcccgtg aagcagagga
      601 cgaggaataa tccttacact caaaaagtaa taaacataaa aagccacttt gtaagtggct
      661 ttttgcttag ttcaagggtc gttttatttg ctgaatggtt caactttctg ttatttccag
      721 aaactgcaaa ataatttgag cggtgatgcc ccagatgagt tcgccatcgt agcgtttgaa
      781 gtaaagcacg gggtgtgact gagactcgtg gacgaaatcc tgatagtagc ggttggcagg
      841 gtctttgagg aatgaaaggg gcacaatgaa ggcacgggca acttcttcgg gtctcaaaaa
      901 taattcctgg ggccaggata tgatgccaac aaccggcgtt agcgcgaaat gggaaatcgt
      961 gccaaacgta ggcatggtgc cgagcacggt aacgctttcg ggtaaaaggc taatttcttc
     1021 aaagctttcg cgcagggcgg tttgaacaag gttcgcgtcg ctttcttccc atgcaccacc
     1081 cggaaaggca acttgtccgg ggtgactcca gagtttctca gtccgtcggg tgagcaaaat
     1141 gtgccattcg ccttgatgac acaaaagtgg aatgagcacc gccgcaggat gaagtgggtt
     1201 ggtgagttgc aggcgtttat caatgatggt gccaggattt gtggcaagat gtgttgccag
     1261 tctttgcgct aaatcagcgg gcagaccttg gcaagcattg agtaagttat cggtcaatcc
     1321 tgatttcctt gttcgttatc gaaagcatca tcgtcgctgt cctcagcagc gagacggtca
     1381 actttgcgcc caaagatgag aaacccggtg tgtccgatca tgcggtcaac gggtctcagg
     1441 cgcgcccagt cggtcttgta gaaacgttgc aaaatttcgc tgacttcaat catggcaaag
     1501 ttggccagtt taagttcgcg aaggataatt tcaacctggt tgaaagtggg taatatcatg
     1561 cccaggctac ccccgccacg caagctttta cgcacatgat ggagataatc ctgtgggttg
     1621 ggcaggtcaa gaatgatggc gtcaacgttt tcttcgtcaa agccgtcttc cgcgttgcca
     1681 actttgaaat caacccggtt ttgcaggccg aacttcgtca ggtttttgat agccaggtct
     1741 tgaatttgcg ctttgcgttc gtaggagtaa acctttccgg ttgtgccaac catgtatgcc
     1801 agcgcggtgg ttaatccgcc tgagccggtg ccacattcaa tgacgcgttt tcctggcccg
     1861 atgcccatgt gaaagagaat atagctaatt tccttggggt acatgatttg ggtgccccgt
     1921 tttaaacctt tgatgagatc gctaaggctg ggctgaagca agtagaaggg attgccttga
     1981 tgggattcca agcgtgtgcc ccaagcggtg ccgatgatat cgtcatggcg aatgacgcca
     2041 cgatgggttt gcaacaccgc gccggcttcg agcacgagta aatggctgcg atgtccggtg
     2101 ccttgcagtt gaacaaggtc gccttcctgg gcaaagttgc tgtcggtgaa tatagctgaa
     2161 ttcatttagg ctatccttcg gggtttatac gggtgaacca gttgccaagg aacaggaaca
     2221 ctaagctgcc gacaatgccg ccagccattt gcccgccacc aacggtcatg aatttgacgt
     2281 cgtagtgtag aaaaacgaga tgtcccaggt aaaaaccaag ccagctaagt gccaaataca
     2341 aaataagttt ccaaaatccc ccaccacgcc aaagatggaa caaggcgcca aagagagcag
     2401 ccagtaaacc accaaaaatg taggatgtga tgttcataag tttcctttag tctaagcggg
     2461 cagttcacgg tgatggtgaa tgatgcgcgc aagttgttgt tttgcagaca tggccagaag
     2521 accagtccaa aagaactcaa cgcacaagcg ctatccgaaa gggtatttgc atcaaaagtc
     2581 tgcccgaggt cttctgaccg cgagcatgac ttttcgttag cactggtata gacaacttgc
     2641 gtgttggtcg tgggcttaat cataacatta agtggtatat tttagctatg tttgcagata
     2701 ctcattgcca tttagacgac agacggttcc aaaatgacct gccccaagtg attaaacgcg
     2761 cggaagatgc cggcctggtt ttgataatca attcaggcgc ggatatggat tcatctgaag
     2821 cttcggtgcg ccttgcccaa caatatgaag gttttatctt cgcgaatgtt ggtctgcacc
     2881 caaaccgcat taatgaactg accgcacaga ctttgaaacg tttgcgattg ttgtcaaaaa
     2941 gccctgaagt gctggcgatt ggtgagattg ggctggatta tcactggaac accttcccgg
     3001 tttggcaaca aaaagaagct tttcgtacac aacttgattt ggctgctgaa atggaattgc
     3061 cagttgtgat tcacagccga aaatcaactg atgacgttgt tgaaatctta ttggaatggc
     3121 atgccggtct gccagcggag catcctcaaa aagcacgacc gggtgccttg catagctgga
     3181 gcgatactta tgaaaaggcc aaacctttga ttgaagcggg tttttacttt ggcgtgagtg
     3241 ggccggtgac ctatcttaac gcgccagaac ggcgggagtg cacggctgcc ttgcccctgg
     3301 aacgcatctt gattgaaacg gattgccctt atctaacgcc acatccttta cgcggaaaac
     3361 gcaacgagcc ggcttatgta cgcaagatgg tcgaggaagt ggcgcgtttg catggcaaga
     3421 cgattgatga aactgcaaaa atcacaaccc aaaatgcttt taggctttat ggcttgagag
     3481 tgcaggtagc ttaaagccag cttgctttag aaaagaatta atgcaataaa aaacgcgggt
     3541 attgtcccgc gttatgtttt aaatggctag ctgtttatgc tgaaaaccct tcgcgtttgt
     3601 aatccacaaa gcgatacccc gtgccacgtt cggtgagaat gtatttgggg ttgctgggat
     3661 ctttttcgag ttttttacgc aagtagttga tgtaaaggcg cacgtaatgg ggttcgtttt
     3721 cgtattcgta accccagact ttgttcagga tttggtcgtg ggtcagcacc caaccggcgt
     3781 ttttcaccaa atggtaaagc aaacggtatt cggtggggcg aagctgaacg agttcgcctt
     3841 cgacccaaac ttcatggcgg tcaaagtcaa tcgtgaggcg gtcgtcaacg acgattttcc
     3901 cggactcttc ttcactcctg gcagcactgc ggcgcaaaac cgcgcggatg cgactggtca
     3961 attcccgtgg gctgaaaggt ttggtgatgt aatcgtccgc gccgagttcg agcccttgaa
     4021 cgatgtcggc ttcttcgctt ttggcagtga gcatgataac cgggatttgg ctttgttcgc
     4081 gaatgagctt aagcacggta aaaccgtcca tgtcaggcat cataatatca agcagcacca
     4141 aatcgggcag ggttgtgcgt aactggtcca tggcttgttg accgttcatg gcttcaacaa
     4201 cgtcaaagcc atcgtgttcc aggttaaggt ggatcacttt tacaagcccg cgttcgtcat
     4261 cggcaactaa tatctttttc tttgttccac tgggttccat ctttcatctc ctagcttatt
     4321 ttgactaaaa tggcgctgat gttatcagta ccgccggcat tattggccat ttcgatgagc
     4381 gaattgacca ttttttcgta atttccgcca tcgttgatgc gctttgacag ggtttcctgg
     4441 tctacgagcc cccaaagtcc atctgtgcaa agtaatagcg tgcatggttc tgtgatttca
     4501 acatgcccga tatcagcccg aaagccttca ttttgcccaa gtgccctaaa cagtacgttg
     4561 cgttgggggt gatcgttggc atcttcctgg ctgatttgcc caagttcgac caaacgttgc
     4621 accaggctgt ggtccagggt gagcgctttt annnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn natgctgctg gcaatatcgc cgtacatatg cccgcccatt ccatcagcaa
     4801 ccataaaaat ccccagcgta gagcggcttt tggggagctg ggttttgatg ttgagcgcgt
     4861 agatggcgtc ttccatgtgg gtgcgggtca ttcccagtga ctgcgccagc tggacttcca
     4921 gatgcacagg gatctgttcg ttgctaaagg cgtttgccag gtctattggt ttgttttttc
     4981 ttctgcgact aaaaattgac aaaattactc cgttacatca ttgttttatg tcttaaggaa
     5041 ggtcttgcct gacgcgttcc aaatagtctt cagccgccca accggtgcga ttggcatcat
     5101 aaggggcttt gagctgccac caaatgagat catcaatgac ttcgggaccg ccaacgactt
     5161 cgaacatctc agcgtccagg gcaagaaagt taatcgtttg accccgtccg ggactgttgc
     5221 gaatgttgag tccaagtcct tttgtgccca cgactttaac gaaactgcca acgccgatgg
     5281 tgtcatcggg taagatgttg ggcgcgggct gagtaggtgt gggcagttgg gtgggtacaa
     5341 aaacagtcgc cgtttgggtg ggtgctgcga ttagggtgat gttataaaca gcttgctggt
     5401 cgggagcgtt ggctttttcc gcgcttttat tgaaaataat atacgcaaaa ccgagcaaga
     5461 ccagggcgaa aataaacgcg cccaggatgg caagcggatg aatcttgtta tttctgcttt
     5521 cggtcataag ttctccgtac ctaattatag cttattaaaa gcggggattg ggatagtgtg
     5581 taaacagaaa ctcaaatgaa acgtgtcgca ataaaagcag tggtaaaatg tctatcatgt
     5641 tgatacggaa aagaaagttt aagccttcac gcccaagacg ccgggcctat agttctctct
     5701 cgcggcgacg gcgcacggct agtggcttgt ttgtgctggc cggcttgttt ttgctggcag
     5761 caatagtttt aacagttatc agcattgtgc agttcaagtc tgtggccgtg gtgtatccaa
     5821 atggctcgca gattggggat attccggttg gtgggctttc gcgcaagatg gctgaagaac
     5881 gcctaagaga tgcttacagt attcccgttg aactgcgtta tggcgacgcg cgcttacagt
     5941 tcagtccgga tgttttaggt ttgacgctga atatcaaaga aactttagat aatgcggatg
     6001 agcaaaaccc aggcagtacc tggtgggctt atttatgggg caagctggag acacccattg
     6061 cttttacact tccgatgcag tttggcgaga accaggcaat ggtgcgtcag gttttggaag
     6121 atacttttcc gaatcgttat gatcaattag caagcgccac gattccagtg gtcaattcat
     6181 atcatgtttt acccggtgaa cctggtttag cccttgccga ttatgacgaa actgttgcac
     6241 agattatgaa cgccttaaag tcgccaaatc aaagggtggt tcagttgcgc gttgaagaaa
     6301 gcccagcctt agccatgctt tgggaaaatt tggaaattaa actacgccag acgattgaac
     6361 aggaaggttt caacggtttg gttgaaattt acctggaaga tttgcatgac ggacgcttaa
     6421 tgcatttcgc cacgcgtggc gggacggatg tgccggttga tgtggcctat tccggggcga
     6481 gcacggtgaa aattccgatt atggtctcca cgatgcgacg tgtttcggat cctatcccgg
     6541 ctactgcaag cacatggatg aaagcgatga tacaggattc gcttaacccg ccagcagacg
     6601 ggctgatgaa aaactatatg gacaacaata ccggtccgtt gatggtaacg gatgatatgc
     6661 gcgagctggg ctaccaaaac acgtttatgg cgggtttttt tgagcctggt tcgcctttgt
     6721 tgtataagta cgagacgccg gcgaataccc gcactgacgt atatctgaac ccggatactt
     6781 ataatcaaac ggtgccatct gagattggcg atttgcttaa gcggatttat ttgtgcgcga
     6841 agcacgacag cccggagtac aatctttttg acggggaagt gaatcagagc gaatgccaaa
     6901 ttatgattga taatttgtta tcgaatcgca tgggagcgct gattgaagtc ggtgttgcgc
     6961 ctgaaggacg tgtggcgcat aagcatggct ggacgaacga gacggatggc ttactgcata
     7021 cgataagcga tgtggggatt gtcttttctc cgggtgggga ttatgttttg gttatcttca
     7081 ttcattccac tggccagctt ctttttgacg aaggtaattt gctttttgca cgactttcgc
     7141 aatccattta taatgcatat aatccttatc agcaggcggt tgtttatata gaataacccc
     7201 aacactggaa ttgatatgcg actaatcatg acccacgaac aggccgattt agacgcgctg
     7261 gcttctatgc ttggcgcgca tatcctgatg cctgatgctt acgctgtttt gcccagacaa
     7321 ataaaccgca acgggcaggc atttctgcgc aaatttggca aggaactggg ctttacacgt
     7381 agccatgatt tacccagaaa gaatattgaa gaaatcattt tggtggatac gcagtcgatg
     7441 gtgactctgc ggggcatcac gcgggatacc aatgtgcgtg ttattgacca tcacgcacgc
     7501 aaaagccgcg aacaagcgga atgggactat gaaattcatg ataccggtgc gtgtacaacg
     7561 gtgctggtcg aaaagattat ggaagcaaat ctgccggtta gcccgcaaga agcgacgctg
     7621 atgttgctgg gaatttacga agacacgggt tcgctgactt atccaaacac tatcgcgcgc
     7681 gacgcgagcg cggtgagtta cctgttgacg atgggagcgg acctgaacat cgcttcaaag
     7741 tacctgaatc cgccactttc gaatgcgcaa atgcagcttt atgaccgttt gctacagaat
     7801 ctcatgacag tgcatatcga gcagagtatg cttgttgtgg catgcgcttc agcgcttgat
     7861 ttgaacgatg agatttccag tgtcgcgcat cgtttgcggg agtttttgga gcccgatggc
     7921 ctgattgtgc tggcactgac gcgcatgggt ttgcgtttgg tggcacgggg aaactcgccg
     7981 gatgtggata tggcaaagct ggccaagcat tttggcggtg ggggacatca gcgcgcggca
     8041 tcggccttga ttcggcttca gagccggcca gtcgctgagc agagtgaatt ggttttgaac
     8101 cactatcaac aaaaagtact tgactttttg ccccatatcg tcagccccag cttgcaggta
     8161 aagcatatca tgtcgcaaaa tcccttgctc ttgtcacctg aaacaaccgt tcaggaagtg
     8221 gcagagttga tgaaccgcta tggatttgaa ggctatcctg ttgttgaaga tggtcgtgtg
     8281 attggtttac tcaatcgccg taatgtagac agggctttgc accacaaact ggatgcgacc
     8341 acgcgcgaat tgatggacag tggcgaagta agcgttgtgc ctgaggacag catttcgtat
     8401 ttgcaggagc tgatgacggc aagtggctgg ggacagg
//
LOCUS       DAOSFC010000019         8351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7301_length_8351_cov_4.847517, whole genome shotgun sequence.
ACCESSION   DAOSFC010000019 DAOSFC010000000
VERSION     DAOSFC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7301_length_8351_cov_4.847517"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PK791_01075"
     CDS             <1..229
                     /locus_tag="PK791_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91938.1"
                     /translation="ATEGMNLTDGENILQSDDPQIFADLVYKAYTDKTLWTKLSDNGL
                     SFVEDRHGLESFETRLQEIIAYLNKPKHQDP"
     gene            245..1999
                     /locus_tag="PK791_01080"
     CDS             245..1999
                     /locus_tag="PK791_01080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH91939.1"
                     /translation="MKLFQSKRNLVNNSGLFDAQYYLDANPDLKKLDIDPLKHFLETG
                     WQKGYNPSAHFDTKYYATVNADVLKENENPLIHYLRKGKKEGRKALPDPNEMWRFSMK
                     AKPFKKTPLLSYRNFQFAINYIKAYGLKSFFNKVLNKIFSRPIFLGLRGSNPVKLLDA
                     DKQFIPLKPRRSTVEPYDVSISVIIPCLNAGDEFQFLLKVLKAQKGFKDIEIIIVDSG
                     STDRTLDFAKRFNAKVIQIKPEEFSHSYARNLGAKHASGDFLLFTVQDALPPSDTWLH
                     EMFTVLSDNQVAAVSCAETPREDADLFYRQICWNHYNFLEVQNADRIFSLPKVQNHIS
                     LRQNGQLSDLANLISRELFEQYQYRLNYAEDIDLGIRLIKDGYKLAFLGTTRIIHSHN
                     RPAYYFLKRGYVDNQFLSDIFSDFEVPKLHIESFVPDIAFVFRFLAELTEKLTTVPFP
                     ISPDFFAGFIENQFSQQLSRKYPEDLPKTPTTHLDTKALSFFETIIGQHGFTLKGKPY
                     NGFLLQAYFGYVNITASYLKTAYETINEQLMSEIISCLYKAFSIVVGAHLAYCAKNRT
                     GDESIDMDALHKTLMAGV"
     gene            1999..3231
                     /locus_tag="PK791_01085"
     CDS             1999..3231
                     /locus_tag="PK791_01085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH91940.1"
                     /translation="MKILYALHQFYPEYQAGTEKFIYNIATMAQLNANKVKVVTYRLG
                     EFSHNEKKSDGVSYEEYFYKSLPILAFKYDQQAFDINFATENPVGLSFAREILQREKP
                     NLLHIGHLMRVFPFAQAALELEIPYILTLTDFFTICPLNTLSPATGSLCNGPNGGEAC
                     ARYCSKFTSQFIKERLESTKRVLSGAKEIFVPSTFVANMIESEMNGIKVTVNNHGIRQ
                     STIQPNYKTYDDHDSLKFASISTLISHKGTHLAIQAFETLKTEHAELDIFGVGEKSYT
                     DWLKSLARKSKVSFKGAFMAENLAKVLNDIDVLIVPSLVYETYSFVVHEALSAEIPVI
                     VSDLGGMTEKVLHGENSYKFPAGNVDALRDIMQKILDKPSDLNRIKSNIRKSTLIPNI
                     EQEYYVYQKAYKQIEKQA"
     gene            complement(3277..4719)
                     /locus_tag="PK791_01090"
     CDS             complement(3277..4719)
                     /locus_tag="PK791_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH91941.1"
                     /translation="MMKKKYLALSVILLLALFLNPMAQPQLTEAKSSQRPQQQQVTAY
                     DLISAMNVLRMSIGNNGLIMDDTINAVAQTTAQIMADQNLSWHIGNVGGRIQAAGYGA
                     GQRIFATENFAMGINLSIDQIMVMWNDPDHMLPATNPHYCHVGAGVATASNGFTYYVL
                     QAAYIAGQPCEGRPPGNPGDPGTGQPVIPGVVTPVELAEMDENGIYTHLVMPGQSFWA
                     IAVAYQVTIKDILAWNNLTESYKLQIGDKLIILSPKAKAYVPPPKLGEVEVSSPAADG
                     SITHVVKPYENLSKIAEAYGVTVKRITELNGIKETTPLGIGWELLIAPSNHTPTPTER
                     PLTPIEMLTPAADGKYYHIVREGQNLNWIADYYGVNLADLLAWNNLKETSVIYPGDKL
                     LLNVTPPPTITPTFTPLTPSPTATMTPTATNTLIPSATPIQTLAESSEVREVNGTGVG
                     KILLPVFAGIAVIVAITLFLRYRRGQKREE"
     gene            complement(4818..6173)
                     /locus_tag="PK791_01095"
     CDS             complement(4818..6173)
                     /locus_tag="PK791_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOH91942.1"
                     /translation="MKRNINTKRLRRQLAPRPLWQRPWVWIVGFVVLVLLAVLVYQIP
                     SVNNRAYFHLARIKARIHYFFNPPDKQVFKPSDQNVLSDEAIANLTAMAPTATPMPTE
                     TSVPLALIEDTPTPTIVPTNTPVPTPLPKKVLLEGIEPEKQGFNNCGPSNLSMALGFW
                     GWEGDQYVTEKVLKPFRQDRNVMPYEMLSYVHLETELGAIVRYGGNLELVKELVANGF
                     PVVIERGMDDPREGWMGHYGIIAGYDDEKQEVTIPDSYLGWIQMSYEDIEMYWAQFDD
                     IYMVIFPYEREHEVHAILGQQMDDAYNKQYALDQVTARLYTTKGRDLFFAWYSRGSIM
                     VEMQDFWGAAESYDRAFEVYEKLPEEERPWRVTWYQTGPLFAYYYMGRYNDVYNLAKA
                     TIDQSPEPAIPESWVWLGRAELKRGNRPQAVWAFRQALKWHPGWWVAENELIALGEPI
                     D"
     gene            complement(6281..7261)
                     /locus_tag="PK791_01100"
     CDS             complement(6281..7261)
                     /locus_tag="PK791_01100"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOH91943.1"
                     /translation="MGVYIVTGTAGFIGSRVSARLLDEGHVVWGVDNLNHVYDVRIKE
                     HRLEQLLGREGFHFLQEDISQRAFIDTLAKHAPQADAVINLAAIAGVRASTTDPWSYL
                     EANTLGALNTLEYARHQKVPKYILASTSSLYGDDNKPPHSETASTDHPLAPYAASKKG
                     ADAFAYSYHHLYGLDVTVLRYFTVYGPAGRPDMVMFRFCQWIAEGKKVTITGNGEQSR
                     GFTYVDDIVSGTLLALKPLGFDVFNLGGHEVVTINELLAMFEERLGKKAVVEYIPSHR
                     ADVFENLADVSKARKDLGWEPKVSLSEGIDAMVDWYLTHRDWAKDIETPY"
     gene            complement(7261..>8351)
                     /locus_tag="PK791_01105"
     CDS             complement(7261..>8351)
                     /locus_tag="PK791_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOH91944.1"
                     /translation="FRATTDYSVLADIDAVSISVPTPLRKTGDPDMSYIASASQQLAP
                     YLHRGMVIVLESSTYPGTTRELVLSNLVENSDLKVGEDFFLAFSPERVDPGRKDYTTY
                     NTPKVVGGITKNCTEVAAAWYGQALETIVPVSTSEVAEMTKLLENTFRMINISMINEM
                     AIMCDRMNIDVWEVVDAAATKPFGFMKFTPGPGLGGHCIPVDPMYLSWKMKTLNYTAR
                     FIDLASQIDSNMSRYVVGKIQDSLNRFKKPLKGSTILLMGIAYKPDINDLRESPALDI
                     IHLLLDKGAIVGYHDPFIPELHEAGLNMASETELVAAVEKADMVAIITNHSKVDYKMI
                     YEKAQLIFDARNALKDIAPAEDIKVVKL"
BASE COUNT         2302 a         2038 c         1793 g         2218 t
ORIGIN      
        1 ggccacagaa ggtatgaatt tgaccgatgg agaaaacatc ctgcaaagtg atgacccgca
       61 gatctttgcg gatctcgtct ataaggctta tacagacaaa actctctgga caaagctatc
      121 cgacaatggc ttaagttttg ttgaagaccg acatggtttg gaaagctttg aaacaaggct
      181 acaggaaatc atagcctatc taaacaagcc taaacatcaa gacccttagg tgtaaaagta
      241 aaagatgaaa ctatttcaaa gcaagcggaa tctggtcaat aatagcggtt tgtttgacgc
      301 gcaatattat ttggatgcaa acccagactt aaaaaagctc gatattgacc cgctaaaaca
      361 ctttttggaa accggttggc aaaaaggtta taacccttcc gcacactttg acacgaagta
      421 ttacgcgact gtaaacgcgg acgttctcaa agaaaacgag aacccgctca ttcactatct
      481 cagaaaaggg aagaaagaag gccgaaaagc tttgccagac ccaaacgaga tgtggcgatt
      541 ttcaatgaaa gccaagccct ttaagaaaac cccgctttta tcctacagga attttcagtt
      601 tgcgatcaac tacatcaagg cttacggcct aaaaagtttt ttcaacaaag tgctcaacaa
      661 gattttttcc aggccaatct ttcttggctt gcgcgggagc aatccggtga aactgctgga
      721 tgctgacaag caattcatcc cacttaaacc cagaagaagc accgttgagc cctatgatgt
      781 cagtatctcg gtcattattc cttgcctgaa cgctggcgat gagttccagt ttttacttaa
      841 agtactcaaa gcgcaaaaag ggtttaagga tatcgaaatt attattgttg actccggcag
      901 cacagaccga acccttgact tcgcaaaacg attcaacgcc aaagtcatcc aaataaaacc
      961 tgaagaattc tcacattcct atgcacgcaa cctgggtgcc aagcatgcca gtggcgactt
     1021 cctgctgttc actgtccagg acgccctgcc accttcggac acctggctcc atgaaatgtt
     1081 caccgtacta agcgataacc aggtcgcggc tgtttcatgc gctgaaaccc ccagagaaga
     1141 tgctgatctt ttctaccggc aaatttgttg gaaccattac aactttcttg aagttcaaaa
     1201 cgcagaccgc atctttagcc tgcctaaggt gcaaaatcac attagtttaa ggcagaacgg
     1261 acaactttcg gacctcgcta acctgatttc gcgcgaactt tttgaacaat accaatatcg
     1321 gctaaactac gccgaagata ttgaccttgg catcaggctc atcaaagatg gttacaaact
     1381 ggcttttctc ggtacaaccc gcatcatcca ctcgcacaat cgtccagcgt attatttcct
     1441 gaaacgcgga tatgttgata accagttctt gtctgatata tttagcgatt ttgaagtccc
     1501 taaactccat atcgaatctt tcgtaccgga catcgccttt gtgttcagat tcttagctga
     1561 acttacagaa aagcttacaa cagtgccttt tccgattagt cctgattttt tcgccggctt
     1621 tattgaaaat caattcagcc aacaactctc aagaaagtat cctgaagatt tacctaaaac
     1681 acccacaaca catcttgaca caaaagcgct cagctttttc gaaaccatca tcgggcagca
     1741 tggctttacc ttgaaaggta aaccttataa tggcttcctg cttcaggcat attttggcta
     1801 tgtcaatatc accgcgtctt atcttaagac cgcatacgag acaatcaatg agcagttgat
     1861 gagcgaaatc attagctgtc tctataaagc tttttcaatt gtcgtcggag cgcatttagc
     1921 ttattgcgca aaaaaccgaa ccggtgatga aagcatcgac atggacgcat tgcacaaaac
     1981 ccttatggca ggtgtttgat gaaaatactc tatgctctcc atcagtttta cccggaatac
     2041 caggctggta ctgaaaaatt catctacaac atagccacaa tggcacaact caatgccaat
     2101 aaagtaaaag ttgttactta cagacttggc gagtttagtc ataatgaaaa gaagtctgat
     2161 ggcgtttctt acgaagaata cttttataag agtttgccca ttttagcttt caaatatgat
     2221 cagcaagctt ttgatatcaa tttcgcaacg gagaacccag ttggactttc cttcgccagg
     2281 gaaattctgc aaagagaaaa accgaatctt ctacacattg gtcacttgat gcgcgttttt
     2341 cccttcgccc aggcagcttt ggaattggaa attccttata tcctgacctt aacagatttc
     2401 tttaccattt gccctcttaa tactctgtct cccgccacgg gcagtctgtg taatggaccc
     2461 aatggtggcg aagcttgtgc gcgttactgt tcaaagttta cgtcccagtt tattaaagag
     2521 cggctcgaaa gtactaagcg tgttttgtca ggcgcgaaag aaatctttgt accttcaacg
     2581 tttgtcgcga acatgattga atccgagatg aatggcatca aagtcaccgt caataaccac
     2641 ggcatacgcc aaagtactat ccaacccaac tacaaaactt atgacgatca tgattcattg
     2701 aaattcgcca gtattagcac actaatttcg cacaaaggta cccatctcgc tattcaagcc
     2761 tttgagacac tcaaaaccga gcatgccgag ttagacatct ttggggttgg cgaaaaaagt
     2821 tacacggatt ggctcaaatc cttggctagg aaaagtaagg tttcttttaa aggggcattt
     2881 atggcagaaa accttgccaa agttttaaac gacattgatg tcttgattgt gccatccctt
     2941 gtttacgaaa cctactcctt tgtcgttcac gaagcactct ctgcagaaat accagtcatt
     3001 gtttcagacc tgggcgggat gactgagaaa gtccttcacg gagaaaacag ctacaaattc
     3061 ccggctggta atgtagacgc tttgcgagat attatgcaaa aaatacttga caaaccgagc
     3121 gatttaaatc gaataaaaag caacattcgt aaaagcacgc ttattccaaa cattgaacaa
     3181 gaatattacg tttatcaaaa agcatataag caaattgaga aacaagccta attaaataaa
     3241 cccgcttttt aaaaatacac ggagagcgct aataatttac tcttcgcgtt tttgtccgcg
     3301 tctatatctc aaaaacaaag ttatcgccac aatcacagca atgcctgcga aaactggtaa
     3361 caggatcttg ccaacacctg tgccattcac ttctctcact tcggatgact ccgcaagtgt
     3421 ttgaatgggg gtcgcactgg gaattagcgt attcgtcgct gttggcgtca tggttgccgt
     3481 gggactcggc gtaagaggcg taaaagttgg ggttattgtg gggggtgggg ttacattaag
     3541 cagcaactta tcacccgggt aaatgactga agtctctttt aaattgttcc acgcgagtaa
     3601 atctgccaga ttcacgccat aataatcggc tatccagttc aggttctgcc cttcacgcac
     3661 gatgtggtaa tacttgccat ccgctgcggg agtaagcatc tctattgggg ttagcggacg
     3721 ttcggttggc gttggcgtgt gattactcgg cgcaatcagc aactcccagc caattcccag
     3781 gggtgtggtt tctttgatgc cgttaagttc ggtaatgcgc ttcacggtaa cgccataggc
     3841 ttcagcgatt ttacttaaat tctcataggg ttttacaacg tgagtaatcg acccatccgc
     3901 agctggtgag ctcacttcca cctcacccag ttttggtggt ggcacataag ctttagcttt
     3961 agggctcaaa ataatcaact tgtcaccaat ttgcagttta taagactctg taagattatt
     4021 ccaggccagg atgtccttta tcgtcacctg atacgccacg gcaatcgccc aaaaactttg
     4081 cccgggcatg accagatgtg tgtaaatgcc gttttcatcc atttctgcca gctcgaccgg
     4141 tgtgacaaca cccggaataa caggctgccc tgtgccagga tcacccgggt ttccgggcgg
     4201 acgaccttcg catggttgcc ctgcgatata agccgcctgt aagacataat aggtaaaacc
     4261 atttgatgct gttgccacac ccgcgcccac atgacaataa tgcgggttgg tcgccggcaa
     4321 catatggtcc gggtcattcc acatcaccat aatttgatca atcgaaagat tgatgcccat
     4381 cgcaaagttc tctgtggcaa aaattctctg cccggcacca taaccagcag cttgtattct
     4441 gccccccaca ttgccaatat gccagcttaa gttttggtcc gccatgatct gtgccgtagt
     4501 ttgcgccaca gcgttaatcg tgtcatccat tatcaaacca ttatttccta ttgacatgcg
     4561 caaaacgttc attgctgaaa ttaaatcata cgcggtcact tgttgttgct gtgggcgttg
     4621 tgaacttttt gcttcagtca gttgtggctg tgccatagga tttagaaaca gcgcgagcaa
     4681 taatattacg gataatgcca gatacttttt cttcatcata aattaatata taccatagtt
     4741 cgagaaagcc attttttgct gcttttttta agattagcca agcaaagttt cacaagcacc
     4801 aggcagcact ctttaagtta atctatcggc tcacccaaag cgatgagttc gttttcagct
     4861 acccaccagc ccggatgcca tttaagcgcc tgtctaaaag cccagacagc ttgcgggcga
     4921 ttgccacgct tcaactcagc ccgacccagc cacacccagg attcaggaat agctggctct
     4981 ggcgattggt caattgtcgc ttttgccagg ttgtagacgt cgttataccg ccccatatag
     5041 taataggcga acaaggggcc tgtttgatac caggttacac gccagggacg ttcctcctct
     5101 ggaagttttt cgtagacttc aaaagcccgg tcgtaagatt cggcggcacc ccaaaagtct
     5161 tgcatttcga ccataatcga accccggcta taccaggcaa aaaacaagtc gcgccccttg
     5221 gtggtataaa gccgcgcggt aacctgatct aatgcatact gtttgttgta tgcgtcatcc
     5281 atttgctgtc ctaaaatcgc gtgcacttca tgttcacgct cgtaagggaa aataaccatg
     5341 taaatatcat cgaattgtgc ccagtacatt tcgatgtctt catagctcat ctgaatccaa
     5401 cccaaataag agtccggaat ggtcacttct tgtttttcgt catcataacc agcgatgatg
     5461 ccatagtggc ccatccagcc ttcgcggggg tcgtccatgc ccctttcaat gacaaccgga
     5521 aagccattcg ccacaagctc tttcaccagc tccaaattac ccccatagcg cacaatcgcg
     5581 cctagttcgg tttccaaatg cacatagctc agcatttcat aaggcatcac attgcgatcc
     5641 tgcctgaaag gcttgagcac tttttcagtt acatactggt caccttccca accccaaaag
     5701 ccaagcgcca ttgaaaggtt ggatggccca cagttattaa agccttgttt ttcaggttct
     5761 ataccttcaa gcaatacttt ctttggcagg ggtgtcggta cgggcgtatt cgtgggcacg
     5821 atggtcggcg taggtgtatc ttcaataagc gccagtggca cggacgtttc agtgggcatc
     5881 ggtgtagccg ttggtgccat tgcggttaaa ttggcaatag cttcgtctga aagtacgttt
     5941 tgatcactcg gcttaaaaac ctgcttgtcc ggtggattaa agaaataatg aatacgcgct
     6001 ttgatacgcg caagatggaa atatgcgcga ttgttaactg agggtatttg gtaaacaagc
     6061 actgccaaca agacaagaac gacgaatccc acaatccaaa cccaaggcct ttgccaaagg
     6121 ggacggggcg ccagttgtct gcgcaagcgc tttgtgttaa tgtttcgttt catagacata
     6181 cgatgtgaat tatatcaggt cttggatact tgaaatttca gcacatcagg ggctgtaaat
     6241 caagagagaa ccttattgca gtcctgacgc gcgcccagca ttaatatggt gtttcgatat
     6301 ccttcgccca gtcgcgatgg gtgagatacc aatccaccat agcgtcgata ccttcgctca
     6361 ggctcacttt tggctcccag cccaagtctt tacgcgcttt gctcacatca gctaagttct
     6421 caaaaacatc cgcgcggtga ctggggatat attccacaac cgctttttta cccaggcgtt
     6481 cttcaaacat ggctaaaagt tcgttaatgg taacaacttc atgcccaccc aggttgaaaa
     6541 catcaaagcc aagcggctta agtgccagca aagtgccact aacgatatca tccacataag
     6601 taaagccacg cgactgctcg ccattacccg ttattgtcac ctttttacct tcagcaatcc
     6661 attgacagaa cctgaacatc accatatctg gtctgccagc cggcccataa acagtaaaat
     6721 aacgcaaaac ggtcacgtcc aaaccgtaaa gatgatggta gctgtaagca aaggcatcag
     6781 cccctttttt actggcagcg taaggtgcca ggggatggtc ggtagacgca gtttcgctgt
     6841 ggggtggctt attgtcatca ccgtaaaggc tcgatgtcga tgccaatata tacttgggca
     6901 ccttttgatg acgggcatat tccagggtat tcagcgcgcc cagtgtgttc gcttcaagat
     6961 aactccacgg gtcggttgtg ctggcacgca cacccgcgat ggcggctaaa ttgatgaccg
     7021 catccgcttg tggggcgtgt ttggccaaag tatcaataaa agcgcgttgt gaaatatctt
     7081 cctgtaaaaa gtggaagcct tcccgtccca ggagttgctc caaacgatgc tctttgatgc
     7141 gcacgtcata tacatgattg aggttatcca caccccaaac cacatggcct tcgtccagca
     7201 aacgcgcgct gacccgcgaa ccaataaagc cagctgtacc tgtaacgatg taaacaccca
     7261 ttagagttta accaccttga tatcttcagc aggggcgatg tcttttaggg cattgcgcgc
     7321 gtcaaagatg agttgcgctt tttcgtaaat catcttgtaa tcaactttgc tgtgattggt
     7381 gataatcgcc accatgtcgg ctttttcaac tgcggcaacg agttccgttt ccgatgccat
     7441 attcagcccg gcttcatgta attcagggat aaacggatcg tgatagccca caatggcgcc
     7501 cttatcaagc aaaagatgga taatatccaa agccggcgat tcgcgcaggt cgttgatatc
     7561 gggtttgtag gcaataccca tcaacaaaat ggtgctgcct ttaagtggtt ttttgaagcg
     7621 gttgagtgag tcctggattt ttccaaccac atagcgtgac atattactgt cgatttgact
     7681 ggccaaatca atgaaacgcg cggtgtagtt gagcgtcttc atcttccatg acagatacat
     7741 ggggtctacc gggatacagt gcccaccaag cccaggtccg ggggtaaatt tcataaaacc
     7801 aaaaggtttc gtcgcggcgg catcaaccac ttcccacacg tcaatattca tgcggtcaca
     7861 catgattgcc atttcgttaa tcatcgaaat attaatcata cggaaggtgt tttcaaggag
     7921 tttagtcatt tctgccactt cagaagtaga gactggcaca atggtttcca gggcttgtcc
     7981 ataccatgct gcagcaactt cggtacagtt tttagtgatc ccgccaacaa cctttggtgt
     8041 attgtaagtc gtgtaatctt tccgtcctgg atcaacccgt tcaggcgaga aagccaggaa
     8101 gaaatcctca cccactttga gatcgctatt ttcaacgaga ttacttaaaa ccagttcgcg
     8161 ggtcgtaccg ggataggtac tggattcaag cacaatgacc atcccacgat gcaaatatgg
     8221 ggctaattgt tgacttgcac tcgcgatgta ggacatatca ggatcaccgg tcttacgtaa
     8281 cggtgtgggc actgaaatag aaactgcatc aatatctgcc aaaacagaat agtcagttgt
     8341 ggcgcgaaaa c
//
LOCUS       DAOSFC010000020         8176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7513_length_8176_cov_4.444650, whole genome shotgun sequence.
ACCESSION   DAOSFC010000020 DAOSFC010000000
VERSION     DAOSFC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7513_length_8176_cov_4.444650"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK791_01110"
     CDS             complement(<1..231)
                     /locus_tag="PK791_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011173217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91945.1"
                     /translation="MSATFPFQTLEISRALLDCRRCPRLVAWREEVGRVKRRAYLDEA
                     YWAKPVPGFGTANARIMVLGLAPGAHGSNRTGR"
     gene            complement(292..1593)
                     /gene="eno"
                     /locus_tag="PK791_01115"
     CDS             complement(292..1593)
                     /gene="eno"
                     /locus_tag="PK791_01115"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOH91946.1"
                     /translation="METTIEYINAMEILDSRGNPTVQVEVILSDGTFGVAGVPSGAST
                     GVHEALELRDGDPKRYGGKGVLTAVDNVNYEIAEELFGLDALEQKAIDELLIDLDGTE
                     NKSKLGANAMLGTSLAVARAAAAAQEMPLYRYIGGVYAHTLPVPMLNIMNGGAHSNWQ
                     STDFQEFMVMPLGAPNFAEGLRWGAEIYHALKSVLKSHGHGTLVGDEGGFAPALKANK
                     EAIELILESIEKAGYKPGSEVCIALDPAVSEIYDEETGLYNLRKEGRKLNSEEMVAFW
                     TEWIDQYPIVSIEDGFAQDDWEGWKAFTAAVGHRLQIVGDDLLVTNPKRIARGIEEKA
                     ANALLVKLNQIGTLTETLQAIDMVRSHGWRAVCSHRSGETEDSTIADLAVAMNMGQIK
                     TGAPARSDRLAKYNRLLAIEQELGSTGKYAGWDALAFDAKR"
     gene            complement(1639..3282)
                     /locus_tag="PK791_01120"
     CDS             complement(1639..3282)
                     /locus_tag="PK791_01120"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOH91947.1"
                     /translation="MGTKFIFFTGGVLSSVGKGVAAASIGRLLKSRGFKVTIQKLDPY
                     LNIDPGTLSPYQHGEVYVLDDGSETDLDLGHYERFCDLSLKKTSTVSAGQIYEEVLNR
                     ERRGDYLGGTIQVIPHITGEIKRRVYLLGESSGADIALIEVGGTVGDIESQPFLEAIR
                     EMRNELPKKDSAFVHCTWLPYLMATAELKTKPTQHSVRELRSIGIMPDMILPRSDHPV
                     DYEIRQKIARFCDVPVEAVVPMVTADMLYEIPLDLEDSHVADILLARLGLEARHEPKL
                     DGWRWMINELKKDRPVAKIALVGKYVELQDAYLSVREALKHAGLFCDLDIEIDWISSA
                     DLPEDTVDERLAGADAIIVPGGFGTRGIEGKMFAARYAREHQVPFLGICLGMQVMALE
                     FARNVMGMKDATSAEFDSKAINPLVDYMPEMKSQERLGGTMRLGLFDIDLAEGSLAEQ
                     CYLTRHISERHRHRYEINGAYREQLEAHGLHVTGLSPEGDLIEIIELEGHPFMVGTQF
                     HPEFLSRPNRPHPLFVGLLQAINEQRGLPTEKLKFGFEN"
     gene            complement(3409..4191)
                     /locus_tag="PK791_01125"
     CDS             complement(3409..4191)
                     /locus_tag="PK791_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOH91948.1"
                     /translation="MESPVEQTKNNQPFKAKQKFEGKVLKTSLQGVLVDIGSDVPAFL
                     HISQIIDVNNPKTAVQNVEDVLKVDDTVTVWIRKVAKDHISLTMVEPLLYEWRDLKPE
                     MAVKGTVIRLESFGAFVEIGAERPGLVHVSELAHSFVRNPQEVVKVGDELDVMILEVD
                     KRKKQIKLSVKALLEAPEEVVVETPKSGRRGKKAKVEVYEEESEELVPDPTYMEIALR
                     QAMDRAEKRKPAIAARAKKQRMIDEDVDDIFTRTLQSKQSDD"
     gene            complement(4432..5121)
                     /locus_tag="PK791_01130"
     CDS             complement(4432..5121)
                     /locus_tag="PK791_01130"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOH91949.1"
                     /translation="MSILFPLPWYAYLIIALLCAVNWAAAFFDNLKLYYISKPFVLIA
                     MIAFFAHSEGLSALRLPFFIGLVFSLLGDVFLIPQRTRFFVTGMGAFAIAHLAYIYGY
                     TQWRVSYWTYIPAFFALVVLVLAFNFYIETQCKEASLPRFQKRLFKAYGVLVAGMALA
                     GWLCLARVGWGRLPAVMAGLGGSFFMVSDFMIALGKLEKRIHRQRFWVIVTYHIAQML
                     ILSSVLMVKPA"
     gene            complement(5118..6041)
                     /gene="era"
                     /locus_tag="PK791_01135"
     CDS             complement(5118..6041)
                     /gene="era"
                     /locus_tag="PK791_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOH91950.1"
                     /translation="MSETEMNQLFKSGFVAVLGRPNAGKSTLLNTLMGQAIAGVSAKP
                     QTTRRRQLGILTSDSAQIILVDTPGLHESNDKLSLAINSEAIVAIQDADIILYMADAN
                     SAPAAMDKKLADLIAGMNPEAKIILVMNKVDSAPAQNLASNQAGYQALFSETPQTLQI
                     SALRAQGVHELLEAIQAALPEGPQYYDAEQITEDFERDIAAEMIRAAAMDNLAQELPY
                     SIAVLVNEYSERDADRVYINATIFVEREAQKGIVIGKGGQKIKQIGTVARRDIEAMNG
                     QSVFLDLNVKVKKDWKDDERFLRQLGLNDKK"
     gene            complement(6044..6640)
                     /locus_tag="PK791_01140"
     CDS             complement(6044..6640)
                     /locus_tag="PK791_01140"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOH91951.1"
                     /translation="MSAIEKNSQEKQPGQKLRQVVLSEPYRALAEDGFYSFETPVGIM
                     RVLSTPDLVHAVELITLDTQGFRPAPARMPIQAELEAQYKAYFAKKLQRFDLPLAIES
                     LSPFTQKVLNLLRDLPFGETCSYQDLAILAGKPNAARAVGTVMARNRWLIAIPCHRVL
                     PVSGKIGMYSAPGGADTKAWLLRHEGHRVENYKIVKGK"
     gene            6912..7724
                     /locus_tag="PK791_01145"
     CDS             6912..7724
                     /locus_tag="PK791_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91952.1"
                     /translation="MSYEEFVQRLNTTIETDQSFFDKLLIKIVRDPHRFTGVFRLSNV
                     KNKLIQYVTQSREIKFGNFMEEIITEYIQRMGYRNLEKRIQADSQNAQYFNVDQLFIK
                     DNSIYLIEQKIRDDHDSTKKVGQFDNFRKKYLLLGKKHPEHEIIATMWFIDDSLVKNK
                     NYYLGKIANETSANLKLSISYGPELFINILGNIEAWNEIYAYLSRNKAERDQESLDIP
                     DFDTSKEIFCALLNLKKERPALFKKLISDDKTYVQLREELFPTGENLKKLLD"
     gene            complement(7721..>8176)
                     /locus_tag="PK791_01150"
     CDS             complement(7721..>8176)
                     /locus_tag="PK791_01150"
                     /inference="COORDINATES: protein motif:HMM:NF037895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91953.1"
                     /translation="GTMTTGAAAKKLGRDYIMIEMDKKYCDYGEVRLASVVYEESPIS
                     KAIFDEKPIKVTVPEMIEAEALFEGETFYLRNGEAIATLCSDGKLNYQDKKIDMHTLA
                     AIARNVKAKRLNGFDYWYVKRDEKLISISHIREDYRIKKGQIWVQPSIF"
BASE COUNT         2154 a         2124 c         1755 g         2143 t
ORIGIN      
        1 acgccccgtg cgattcgagc catgcgcgcc aggcgccaag cctaaaacca taatgcgcgc
       61 gttagctgta ccgaatcctg gcacaggttt cgcccagtaa gcctcgtcaa gataagcccg
      121 tcgctttacc cgtccgactt cttcgcgcca ggcgaccagt cgcgggcatc gtcggcaatc
      181 caacaaggca cggctaatct caagggtctg aaatggaaat gtggctgaca tcaaaattcc
      241 ttgttaaata aagagagatg gctccagcga accatctctc caaagttcaa attagcgttt
      301 ggcgtcaaaa gccagggcat cccagccggc atatttgcca gtgctgccca gttcctgttc
      361 gatggcgagc aagcggttgt atttggcaag gcggtcagag cgggccggcg caccggtctt
      421 aatctgcccc atgttcattg ccacagccag gtcagcgatg gtgctgtctt cggtctcgcc
      481 ggagcgatgc gaacaaaccg cgcgccaacc gtgcgaacgc accatgtcga tggcttgcag
      541 ggtttcggtg agtgtgccga tttggttcag tttcaccaaa agcgcgttgg cggctttttc
      601 ttcgataccg cgggcgatgc gtttggggtt ggtcaccaac aggtcatcac caacaatttg
      661 caagcgatgg ccgaccgcgg cggtgaatgc tttccagcct tcccagtcat cctgagcaaa
      721 gccatcttca attgaaacga tgggatattg atcaatccat tcagtccaga aagcgaccat
      781 ttcttcggaa ttcagctttc tgccttcttt gcgcaggttg taaaggccag tttcttcatc
      841 gtaaatctca gaaactgcag ggtcaagcgc gatgcagact tcggagccag gtttgtaacc
      901 cgctttttcg atgctttcca gaatcagttc gatggcttcc ttgttcgctt tcagcgcagg
      961 ggcaaaacca ccctcatcac cgaccagggt accatgaccg tgcgacttca aaaccgattt
     1021 cagggcgtgg taaatttcag caccccagcg caggccttcc gcgaaattcg gcgcgcccag
     1081 gggcatgacc ataaattcct ggaagtccgt gctctgccag ttgctgtgcg cgccaccgtt
     1141 catgatgtta agcatcggca caggcaaagt gtgcgcgtaa acgccaccga tgtagcgata
     1201 aaggggcatt tcctgcgctg cggcggcagc acgcgccaca gccaggctgg tgccaagcat
     1261 ggcgttcgcg ccaagtttcg atttgttttc ggtaccgtca aggtcaatca gcaactcgtc
     1321 gattgctttt tgctccaggg catccaaacc aaaaagttct tcggcaattt catagttcac
     1381 attatccaca gcggttagaa cacccttacc gccgtagcgt ttagggtcac catcgcgcag
     1441 ttcaagcgct tcgtgaacgc cagtagacgc gcccgaaggc actcccgcca cgccaaatgt
     1501 gccgtcgctg agaataactt caacctgcac agtgggatta ccacgtgagt ctaagatttc
     1561 cattgcattg atatattcaa ttgttgtttc catattttcc tctttttaaa aaatcagtaa
     1621 aaagcaacaa aatgctgctt agttttcaaa tccaaatttt agtttttcgg ttggcaaacc
     1681 acgttgttcg ttgatagctt gcaaaagccc aacaaacaaa ggatgtggtc ggttcggacg
     1741 cgataaaaat tcggggtgga actgcgtgcc aaccatgaac ggatgtcctt ccagctcgat
     1801 aatttctatc aggtcaccct cgggcgaaag tcccgtcaca tgcagtccat gcgcttcaag
     1861 ttgttcgcga taagcgccat taatttcata gcgatggcgg tgtcgctcgg aaatatgtcg
     1921 ggtcagatag cattgctccg ccagactacc ttcggccagg tcaatatcaa acaaacccag
     1981 gcgcatggta ccacccaaac gttcctgtga cttcatttcc ggcatataat ccaccagcgg
     2041 attaatcgct ttgctgtcaa actctgctga tgtcgcgtct ttcatgccca tcacgttgcg
     2101 cgcgaattcc aaagccatca cttgcatacc caggcaaatc cccaaaaagg gaacctgatg
     2161 ctcgcgggca tatcgcgcgg caaacatctt tccttcaata ccgcgtgtgc caaatccacc
     2221 cggcacaata atcgcgtctg caccggccag tcgttcatca acggtgtctt cgggcaaatc
     2281 cgcgcttgaa atccagtcaa tttcgatatc caaatcgcag aacaaacccg cgtgtttcaa
     2341 agcttcgcgc acactcaaat aggcgtcctg cagttccaca tactttccga ccagagcaat
     2401 tttagccaca ggccggtcct ttttaagctc attaatcatc cagcgccagc catccagctt
     2461 gggttcgtgc ctggcttcaa gccccaggcg cgcgagtaaa atatcggcaa cgtgactatc
     2521 ctctaaatcc aggggaatct cataaagcat atccgctgtt accatcggca ccacagcttc
     2581 cacgggcacg tcacagaaac gggctatttt ttggcgaatt tcataatcca ctggatgatc
     2641 actgcggggc aaaatcatat ccggcataat accaatcgaa cgcaattcac gcacagagtg
     2701 ttgtgtgggc ttcgttttga gttctgccgt cgccataaga tagggcaacc aggtgcagtg
     2761 gacaaaagct gaatcctttt tgggaagttc attgcgcatc tcgcgaatcg cttccaaaaa
     2821 aggttggctc tcaatatcgc ccaccgttcc gccaacttca attaaagcga tatcagcacc
     2881 agaagattca cccagcaaat acacgcggcg tttaatttct ccggtgatgt gcggaataac
     2941 ctgaatggtg ccacccaggt aatcaccacg tcgctcgcgg ttcagcactt cttcgtaaat
     3001 ctgaccggca gaaaccgtgc ttgttttctt gagactcaaa tcgcaaaaac gctcgtaatg
     3061 acccaggtca aggtcggttt ccgagccatc gtccagcacg tacacttctc cgtgttgata
     3121 ggggcttaag gtgccggggt caatattaag ataggggtca agtttttgga ttgttacctt
     3181 aaatcctcgt gatttcaaca aacgcccaat cgacgccgct gcgacgcctt tgccaactga
     3241 agacaagact ccaccggtaa aaaagataaa cttggttccc atttcgctcc gttttttata
     3301 atcgtttcaa cttaatagct ttacttaaaa gttgcgggga gggcttttta tcagctcctc
     3361 cccggattgt tcaaagaatt agtcccaaat actgccagcc gaacatcatt aatcgtctga
     3421 ttgtttgctt tgcagggtgc gggtgaagat atcatcaaca tcttcatcaa tcatgcgttg
     3481 tttcttagca cgggcagcga tggcaggttt gcgtttttcg gcacggtcca ttgcctggcg
     3541 caaagcgatt tccatgtatg tcggatctgg aacaagctct tcggattctt cttcatacac
     3601 ttcaaccttg gcttttttgc cacggcgacc ggatttcggc gtttcaacaa caacttcttc
     3661 tggcgcttcc aggagtgctt tcacgctgag tttaatttgc tttttgcgtt tgtcaacttc
     3721 caaaatcata acatccagtt cgtcgccaac tttcacaact tcctgtggat tgcgcacaaa
     3781 gctatgtgcc agttcgctca cgtgtacgag accgggacgt tctgcgccga tttcaacaaa
     3841 agcgccaaag ctttccaggc ggatcacggt gcctttaact gccatttcgg gtttaagatc
     3901 acgccattca taaagcagcg gctcaaccat cgtcaaggag atatggtctt ttgccacttt
     3961 tctaatccaa acagtaacgg tatcgtcaac tttaaggaca tcttcgacgt tttgcacagc
     4021 ggttttaggg ttatttacat caatgatctg ggaaatatgc agaaatgcag gcacatctga
     4081 accaatatca accagcacgc cttgcagcga ggtctttaac acctttcctt caaatttttg
     4141 ttttgccttg aaaggctgat tatttttggt ttgttcgact gggctttcca tttctctact
     4201 cccattaagg tatattcaca accgcttagt atacactcgc aaacagaaca tgtcaaactc
     4261 tttgacatta tccgcacgaa cataagctcg tctttttttg ctgatacaag cttagatagg
     4321 gtatttaatt acgtttctat tctaaaatat ctggtcgttt ttttcaaggc tagctaacaa
     4381 acattaaata ttatttattc aagctaaaac acaggaaaaa acttacccca cctacgctgg
     4441 cttcaccatc agcacgctgc taagaataag catctgcgcg atgtgataag tcacaatcac
     4501 ccaaaaccgc tggcggtgaa tgcgcttttc cagcttgccc agcgcgatca taaaatcgga
     4561 gaccataaaa aagcttccgc ccaatcccgc catcaccgcg ggcaaacgcc cccaacccac
     4621 acgcgccaaa cacagccatc ctgccagcgc catgcccgcc accagcaccc cataagcctt
     4681 gaacaaacgc ttttgaaacc gcggcaaact ggcttcttta cattgtgttt cgatataaaa
     4741 attgaacgcc aagaccagca ccaccaaagc aaaaaaagct ggaatatagg tccagtaaga
     4801 aacccgccat tgtgtgtaac cgtaaatgta cgccaaatgc gcaatcgcga aagcgcccat
     4861 tcctgtcaca aaaaagcgtg tgcgttgcgg gattaaaaac acatcgccca gcaatgaaaa
     4921 gaccagacca ataaagaaag gcaaacgcag tgccgaaagc ccctcgctgt gcgcgaaaaa
     4981 ggcaatcatg gcgattagaa cgaagggttt gctgatgtaa tacagcttca agttgtcgaa
     5041 aaaagccgcg gcccagttca ccgcgcaaag caacgcgata atcagatagg cataccaggg
     5101 caaaggaaaa agaatgctca ttttttatcg tttaacccca actgtctcag aaagcgctca
     5161 tcatctttcc agtctttctt tacttttacg ttcaaatcca aaaacacact ctgaccgttc
     5221 atcgcctcga tatcccgcct tgcgaccgta ccgatttgct tgattttttg cccgcctttg
     5281 ccaatcacaa tccccttttg agcctcacgc tccacgaata tcgtcgcgtt gatgtagacc
     5341 ctgtccgcgt cgcgttcact gtattcattg accaaaacag cgatactgta aggcagttcc
     5401 tgtgccaaat tgtccatcgc cgcggcgcga atcatctccg ctgcgatatc ccgttcgaaa
     5461 tcttcggtaa tctgttcggc gtcgtaatac tgcggacctt cgggcaaggc agcctgtatc
     5521 gcctccaaaa gctcatgcac accctgggca cgcaaagcgg aaatttgcaa ggtttgcggt
     5581 gtttcggaaa acaaggcttg gtagccggcc tggtttgatg ccaaattttg cgctggggca
     5641 ctatccacct tgttcatcac caaaatgatc ttcgcctctg ggttcatgcc cgcgattaaa
     5701 tctgccagtt tcttatccat cgccgctggc gcgctgttgg catccgccat atacaggatg
     5761 atatcggcgt cttgtatggc aacgatcgcc tcactgttaa tcgccagact gagtttgtca
     5821 tttgactcgt gcaaaccggg cgtgtctacc aggatgattt gggcgctgtc gctggtgaga
     5881 atgcccaact gccggcgacg cgtggtttga ggcttggcac tcacgcccgc gatggcttgc
     5941 cccatcagcg tatttaacag cgtgctttta cccgcgttgg gtcgcccaag taccgccaca
     6001 aaaccagact taaagagctg attcatttct gtttcactca tagctattta cccttaacga
     6061 ttttatagtt ctcgacgcga tggccttcgt gcctcaacaa ccacgctttt gtatccgccc
     6121 cgcctggtgc tgaatacatc cctattttac ccgaaaccgg caaaacgcgg tgacatggaa
     6181 tggcgattaa ccagcggttg cgcgccatta ctgtgcccac cgcgcgggcg gcatttggct
     6241 tgccagccag aatagccaga tcttgatagc tacaggtttc accaaagggc aaatcccgca
     6301 aaagattcag cactttttgc gtgaaggggc taagcgactc tatcgccaat ggcaaatcaa
     6361 agcgttgcag ctttttggca aaataagcct tatattgcgc ctcaagttcc gcctgaatgg
     6421 gcatcctggc tggcgcggga cgaaaacctt gtgtgtccaa agtgatcaat tcaaccgcgt
     6481 gtaccaaatc cggcgtgctg agaacgcgca taataccgac aggagtctca aaactgtaaa
     6541 agccatcctc tgccaacgcc cggtatggct ctgacaaaac aacttgcctg agcttctgcc
     6601 ctggttgctt ttcctgtgaa tttttctcta tagcactcat ttgaaacgtc tcttgattag
     6661 cgaattgtta ctaatgtagg gtaatcgtag gttaatcgtc aagcataaaa agtagctata
     6721 ttgtaatata gaatacgaaa gggcgacttc tcttcttctc ctccttaata ctcagtgcca
     6781 cggtcggcgt ccgtggcact cgtgttttat atacaaacat atccagtcgc aatcaaatat
     6841 cttgtacatt tcggtcctgt tcttgcacaa atcatcatta ataattctct ttttaggtga
     6901 ttgccatgat catgtcttac gaagaattcg ttcaacgtct aaatacaaca atcgaaacag
     6961 atcaaagttt ttttgacaag ctgttaatca aaattgtaag agatccacat cggttcacag
     7021 gtgtattccg tttgagtaac gtaaaaaata agcttattca atacgttact caaagccgag
     7081 aaattaaatt cggtaatttc atggaagaaa tcattacgga atatatacag agaatgggtt
     7141 accgaaactt agaaaagcgc atccaagcag attctcaaaa tgcccaatat tttaacgtag
     7201 accaattatt cataaaggat aattctatct atctgataga gcagaaaatc cgcgacgacc
     7261 atgacagtac aaaaaaagtt gggcagtttg ataatttcag aaagaagtac ttactcctgg
     7321 gaaaaaagca cccagaacat gagattatcg ccaccatgtg gttcatcgac gatagtttgg
     7381 ttaaaaacaa gaattattat cttgggaaaa ttgccaatga gacttccgct aacctaaagc
     7441 tatccatcag ctatggacct gaattattta tcaacattct ggggaatatt gaagcatgga
     7501 atgaaatcta tgcttatcta agtagaaata aagctgaaag agaccaagag tctctagata
     7561 tcccagattt tgataccagc aaagagattt tctgcgcgtt attaaatcta aagaaagaac
     7621 gccctgcact ctttaagaaa ctaatttcag atgataaaac gtatgtacaa ttgagagaag
     7681 agttattccc aactggcgaa aatctcaaga aactgcttga ctaaaagata ctaggctgta
     7741 cccaaatctg tccttttttg attcgataat cttctcgaat atgggatatg gaaattagct
     7801 tttcgtctct tttcacatac cagtaatcaa aaccgtttaa ccgtttggct ttaacatttc
     7861 ttgcaatggc agccaaggtg tgcatatcaa tttttttgtc ttggtaattt agctttccat
     7921 cgctacatag agttgcgatt gcttctccat ttcgcaaata gaatgtttcg ccttcgaata
     7981 atgcttcagc ttcaatcatt tctggaacag taaccttaat cggtttttca tcaaaaatag
     8041 cttttgaaat gggtgattct tcataaacaa ctgaagccag tcgcacttcc ccatagtcgc
     8101 aatacttttt atccatttca atcatgatgt aatcgcgccc taatttctta gcagcagccc
     8161 cagtcgtcat tgtccc
//
LOCUS       DAOSFC010000021         8145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7553_length_8145_cov_6.029172, whole genome shotgun sequence.
ACCESSION   DAOSFC010000021 DAOSFC010000000
VERSION     DAOSFC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7553_length_8145_cov_6.029172"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(541..804)
                     /locus_tag="PK791_01155"
     CDS             complement(541..804)
                     /locus_tag="PK791_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91954.1"
                     /translation="MNVCPNCAFIIWNKTENCPKCGLSFSAAPAIDLENEQVYRAVVA
                     SSAIKNTMEMEKVRRWVIAIGISSLVSGLAVLIIVLLAVISLW"
     gene            complement(831..1814)
                     /locus_tag="PK791_01160"
     CDS             complement(831..1814)
                     /locus_tag="PK791_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91955.1"
                     /translation="MRKVKVNCISILMVLALFACSPQNGGLARNPVAETIAAMDMEAT
                     LIAHELIETQLAGLTNACPNEIETPYGLIRPDCSVTVAERLIYDTEQIKVTIKSLYFD
                     TSSAPALLFWVENNTYGGLNLRAVDLQINGFMMKGAFNARIPAQSQGLVALWVASDAL
                     AAAGVETIQYFEFNLAIDESQNWSQSITTERIRINTNAAPGGVLALNRRDIQLLDREG
                     IRVYLNRYENPTHYTGAQFLIMVENNTDIDIELTALDLKINDHECESSYETHVLAGLK
                     SVEELSLPEDQLATNNIERVETLVFRLQVMRKDTRTLIFNTDFIELVFEQP"
     gene            complement(2146..3126)
                     /locus_tag="PK791_01165"
     CDS             complement(2146..3126)
                     /locus_tag="PK791_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91956.1"
                     /translation="MSKRNKIFALLLMAILLVACAGGAKKRETEKEAIFEVLSSKFIE
                     MLSKNDSTKDNDDEPDNTHEEPTVRDGDDYAINEDVNVQESVIYDDGGIKVTVLSLDF
                     RGWMGPELRFLIENNSEKAIMLQASDLVINGYMINPIMSAEVEAGEKVNDELTVLNSD
                     LESAGIETIQYFSFNLRVIDSADWAETFITDTIRIETDANPAFIQKVDDSGTVLVEQN
                     GVRIIMKELLTEQEFWGAELLLFIENDSGQDISVTVKDVTANGLAVNPLFYADVLSGT
                     KAFAGLGFLENDLKEKGISDIREIELYFNVIDKNNFRAVFKTDKITITFD"
     gene            complement(3264..4994)
                     /locus_tag="PK791_01170"
     CDS             complement(3264..4994)
                     /locus_tag="PK791_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH91957.1"
                     /translation="MKRRMNLINTLVIVLAVLAVSVAVTFIYNRMWLGNQLTWMRETI
                     RFYSSVVDADTENQIEILKKLNSKYRSVRVTLIDPNGDVIYDSDINVNEFRLENHAER
                     KEFREALLGKLGEDIRRSESVGEDSFYFAMRVEGGNVVRLSQQWKNMAGLLGKAMPTT
                     AVVAVLAIIISAFFTHHTSKRILEPIDYYAENLEQIMLAETEDIPVYDELLPFVRELR
                     VQKKTIDAHLEELKYQSETINRLIRDAREGMVLMDSDLTILSINTSAVRLLEGDVGSE
                     YRGKDFLYLCRLPEIYHKLEQSSDSEHETFTVEMSSTLLRIYINRVLRQDELYGVMMI
                     IADDSENLRLEKQRKEFTANVSHELRSPLTAINGYAELLKNGMVDADDVKRIGGTISD
                     EGRRLSAIIDDIIKLSQFDESKVQHVSTEIDLDAYLQQILAVYKEPISQRALKLSITL
                     EGEKTLFFPEAMLHDVLDNLISNAIKYNRDGGGIDVWVSNQANELRLRVADTGIGIPE
                     EHIGRVFERFYMVDKARSRAISGSGLGLAIVKHVVEFIGGKVFLESEVNQGTTIEVFL
                     PKTDLNSDEG"
     gene            complement(4991..5662)
                     /locus_tag="PK791_01175"
     CDS             complement(4991..5662)
                     /locus_tag="PK791_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH91958.1"
                     /translation="MIYCIEDDQSIRELLAYALQKEGYETRLFEDGSTLLEVISEKTP
                     ELIILDIMLPGESGLALLKKLKADPDTKEIPVILLTALGGEYDKVIGLDLGADDYISK
                     PFSVMELLARIRAVKRRSNSTIIQHLEYNGIEINYLNRTARVDGEELDLTFKEFELLF
                     QLFSHPGMAYSREKLLESVWGYDFEGGTRTVDVHVASLRKKLGSKGSLIKTVRNIGYK
                     AGAEA"
     gene            complement(5672..6331)
                     /gene="phoU"
                     /locus_tag="PK791_01180"
     CDS             complement(5672..6331)
                     /gene="phoU"
                     /locus_tag="PK791_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOH91959.1"
                     /translation="MMRAKYFKELEELKTEVILYGSYIEGMCRTLQKAFVEGDYEEAK
                     LMVDEESTIRSKERRIENDCLRIMSTQQPVAKDLRFISSVLKIANNLQRIGGHVIEIV
                     ELLDFVGPINNLFAVNQLIDMNSRVMVMLKKALDSFVENDLELAEEVIASDNEVDALF
                     DKTMDEIADRIRTDTDSYKYLIDAMQITKYLERIGDNTERIARWVIFAIKGDPEQQEQ
                     H"
     gene            complement(6339..7097)
                     /gene="pstB"
                     /locus_tag="PK791_01185"
     CDS             complement(6339..7097)
                     /gene="pstB"
                     /locus_tag="PK791_01185"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOH91960.1"
                     /translation="MTDIKIKIENMDLFYGDFQAIKNANLDIPANEIMAFIGPSGCGK
                     STLIKSLNRMNDLIENCKITGKILIDGEDIYAPGVDVNALRKRVGMVFQTPNPFPISI
                     YDNIAYGPRVHGLRNRARLDEIVEDSLRKAAVWDELKDELRKNALQISGGQQQRLCIA
                     RALAVEPEILLMDEPTSALDPISTGKIEDLVIELKEKYTIVIVTHNMQQAARISDKTA
                     FFLTGEIIETGETGQIFSYPKDERTENYITGRFG"
     gene            complement(7094..7903)
                     /gene="pstA"
                     /locus_tag="PK791_01190"
     CDS             complement(7094..7903)
                     /gene="pstA"
                     /locus_tag="PK791_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOH91961.1"
                     /translation="MSKVLRFLVKFAAILTVAIFLIVVGYIAIRGIPGLSWKMFAWKY
                     TSENLSLMPAMITTIMLIILVLIIAIPFGVLTGIYLTEYARQQSKVVIAIRLATETLA
                     GIPSIVYGLFGMLFFVFRLQWSYSLIAGAFTAAIMVLPLIIRTTEEALLAVDPLLKQA
                     SLGLGATKLQTIVKVCLPVAMPGILAGVILSIGRIVGETAAFIFTLGTMAQLPTRLTE
                     SGRTLALHMYVLSSEAFHVREAFATALVLLVIVLIINRLSTRLSKLVNKTK"
     gene            complement(7896..>8145)
                     /locus_tag="PK791_01195"
     CDS             complement(7896..>8145)
                     /locus_tag="PK791_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOH91962.1"
                     /translation="ILGIGRAIGETMAVYMVAGNQPRLPKSLTSGVRTLTTNIVLEMA
                     YAGVEHRQALIATAMVLFIFILVINFLFFLVKRRDRHE"
BASE COUNT         2288 a         1911 c         1586 g         2360 t
ORIGIN      
        1 tttcgtcgaa gatctgggtc atgccgatct ttcttccaat aagccctttt aacatactat
       61 tctcctgttt gtaatttagc gagactgtca gcctgggcta ggctgcatca tctccgtgca
      121 attcagttaa tgaagctctg tgtagcgatt ctgcccacgc ggcaacattc ttaaactgcc
      181 aatgtcaaaa catgccccga aaggggcaac ttatcattta tatcacacct cgtcatacag
      241 cgcaagggtg atttctgatt ttgttttgac tttttgcctg gtttttcgcc aagcttgcct
      301 attataaggt agagcgtgaa attaagcgag tcttttccgc aaaataatta gccaaaatta
      361 gcccgaaata ttgatgacaa taaacgaata ctaagcccgc ccgtcagcac ttggcttgca
      421 cgcaaagcaa actcaaactt acgtctcgtt cgccaaggtc tccttgccgg cacaagtttc
      481 caagccttca tttttggata gtatgcgttc aacttcagcg agctgtaaat atttgagaaa
      541 ctaccataag ctaatcactg ctaacaaaac aataatcaac acggccaaac cactcaccag
      601 tgagctgatt cctatcgcaa tcacccaccg acggactttt tccatctcca tcgtgttttt
      661 aatcgcggat gacgccacca cagcgcgata aacttgttca ttctccaaat caatcgctgg
      721 cgcagcagaa aaggacaagc cacacttcgg gcaattttca gttttattcc agatgataaa
      781 tgcgcagttt ggacaaacat tcataagact ctccttttgg caaaaaaata ttagggttgt
      841 tcgaacacca gttcaatgaa atctgtattg aaaataagcg ttctggtgtc tttacgcatc
      901 acttgaaggc gaaaaaccaa cgtttcaacc cgctcaatat tatttgtggc gagttgatct
      961 tccggcaggc ttaattcttc aacggacttc aagccagcca gtacatgtgt ttcatacgag
     1021 gattcacatt catgatcatt tattttcaaa tcaagtgccg ttagttcaat gtcaatatcg
     1081 gtattgttct caaccataat taaaaactgc gctcctgtat aatgagtggg gttttcataa
     1141 cgattaagat aaacacgaat cccttcgcga tccaatagtt gtatatctcg tcgattaagt
     1201 gcaagcacac caccaggggc agcattggta ttaatccgaa tacgttcagt tgtgatggat
     1261 tgactccagt tttggctttc gtctatggca aggttgaact caaagtattg gatggtttcc
     1321 acacctgcag ctgcaagggc atcactcgca acccacaagg caactaaacc ttgggattgc
     1381 gcgggaatcc tggcattaaa agcacccttc atcataaatc cattgatttg taagtctact
     1441 gcccgcagat ttagcccgcc ataggtgttg ttttcaaccc aaaacagaag cgctggggct
     1501 gaactggtat cgaagtaaag tgacttaata gtcactttaa tttgttccgt gtcataaatg
     1561 agtctttcag caactgtaac tgagcaatct ggtctgatta agccatatgg agtttcaatc
     1621 tcatttggac aagcatttgt aagtcctgct agttgggttt cgattagctc atgtgcaata
     1681 agggtggctt ccatgtccat tgcagcaatc gtttcagcaa ccgggttcct tgctaaccca
     1741 ccgttttgtg gtgaacaggc aaacaaagct agcaccatca aaatcgatat gcaattaact
     1801 tttacttttc tcattccctg gcctctaaga attattgatg gaaataatgt ttatcaaatt
     1861 atacaacaag ttttcgcttt aatacatgtg gtttttacgt catgcttaaa tttggatagc
     1921 aattaatgca ccttgggtat tttttattaa acacatgcgc aataaaccac ttgtcaaaac
     1981 gctttcgcca aaagcaaata atactcaagc aaaacaacgc aacgagatag cgacagaaat
     2041 gataacagta cgtggcgaga atgccacgca ccgataatta gtttcgtact gagaaagttt
     2101 acgtgcttag cagaccatgt aaaccttgta agttattatt tccatctagt caaatgtaat
     2161 tgtgatttta tcagttttaa aaacagctct aaagttgttc ttgtcaataa cattgaagta
     2221 gagttcaatt tctctaatat cgctaatgcc cttttctttt aaatcattct ccaaaaagcc
     2281 gagaccagca aaggctttcg tgcctgacaa aacatccgca tagaaaaggg gattaaccgc
     2341 caatccattt gctgttacgt cctttacagt aacagaaatg tcctgccctg aatcgttctc
     2401 gatgaacaac agaagctctg caccccaaaa ctcttgttca gtaagcagtt ctttcataat
     2461 gatgcgcacc ccattttgtt caaccaaaac ggtgccactg tcatcaactt tttgaatgaa
     2521 agcagggttc gcgtcggtct ctatacgaat cgtatccgtg ataaatgttt ctgcccagtc
     2581 tgcgctgtca atgacacgca ggttgaacga aaaatattgg attgtttcaa ttccggcgct
     2641 ttcaagatcg ctgttcaaaa cagtcagttc gtcattaacc ttttcccccg cttccacttc
     2701 ggcagacata atcgggttta tcatgtaacc gttgatcaca aggtcgcttg cttgtaacat
     2761 aattgctttt tctgaattat tttcgatcag aaatctgagt tctggaccca tccaaccgcg
     2821 gaaatccaac gacaaaacgg ttacttttat gccaccatca tcataaataa cgctttcctg
     2881 gacattgaca tcttcattaa tcgcgtaatc atccccatct ctaacagtcg gctcttcatg
     2941 tgtgttgtct ggttcgtcat cattatcttt ggttgaatca tttttagaca acatttcaat
     3001 aaactttgat gaaagtactt caaagatagc ttctttttca gtttctcttt tctttgcccc
     3061 acctgcacaa gcaaccaaca gaattgccat cagtagtaaa gcaaaaattt tgttcctttt
     3121 tgacattttc tatcctccgt tattgaaacg cttatcagct aaacaaattt tttaaaagtc
     3181 cttttatctt ccattaataa tggtgtgaaa atttcaagcc cacaaaatgt gggcttgaaa
     3241 tcgggagaat aaaataatgg tcattatcct tcatctgaat tcaggtctgt ttttggcaag
     3301 aaaacttcaa tcgtcgtacc ttggttcact tcgctttcca aaaagacctt acctccaata
     3361 aattcaacca catgttttac aatcgccaga cctaatcctg aaccagaaat cgctcttgat
     3421 ctggctttgt ctaccatata gaaacgctca aaaacacgcc caatatgctc ttccgggata
     3481 ccaatgccag tatcagctac acgcagacgg agttcattgg cttgattgct aacccaaacg
     3541 tcaataccgc ccccatcacg attatacttt atggcgttag aaatcaaatt atccaacaca
     3601 tcatgcaaca tcgcttcggg gaagaacaaa gtcttttcac cttccagggt gatgctcaat
     3661 ttgagtgcgc gttggcttat aggttcttta tacactgcca atatctgttg taaataggca
     3721 tccaggtcaa tttcggtgct aacgtgttgc accttgcttt cgtcaaactg tgaaagttta
     3781 ataatgtcat cgatgatagc cgacaaacgc cgcccctcat cagaaatcgt gcccccaatg
     3841 cgtttcacat cgtcggcatc taccatgcca tttttcaaaa gttcagcgta accattaatt
     3901 gccgtcagcg gactgcgcaa ttcatggcta acgttggcag taaattcttt gcgttgtttt
     3961 tcgagtcgca aattctctga gtcatcagct atgatcatca tgaccccata taattcatct
     4021 tgtcttagga cacggttgat ataaatacgc aaaagcgtac tgctcatttc taccgtgaag
     4081 gtttcatgct cactgtctga gctttgttcg agtttatggt aaatttccgg caaacggcat
     4141 aagtacaaaa agtctttgcc acgatattct gaacccacat caccttccaa caagcgcacc
     4201 gcgctggtat taatgctaag gatagtgaga tcggaatcca tcaacaccat gccttcgcgc
     4261 gcgtcgcgga ttaagcggtt gatggtttcg ctttgatatt tgagttcttc caggtgtgcg
     4321 tcaatagttt tcttttgtac gcggagttcg cgcacaaaag gcaaaagttc atcataaacc
     4381 gggatgtcct ctgtttcggc aagcataatt tgctcaaggt tttcagcgta ataatcaata
     4441 ggttccaata tccgctttga cgtatgatgg gtgaaaaaag cacttatgat aatcgccagc
     4501 accgcaacaa ccgcggttgt tggcatggct ttccctaaca agccagccat gttcttccac
     4561 tgttgcgaaa gacgcaccac attgccccct tcaacacgca tggcaaagta aaagctatct
     4621 tcgccaacac tttcagagcg tcggatgtct tcgccgagtt tacccagcaa agcctccctg
     4681 aattcttttc gttcagcatg gttctcaagg cggaattcgt tgacattgat atcgctgtca
     4741 taaataacat cgccgtttgg gtcgattaag gttacccgta cacttctata tttgctgttt
     4801 aactttttaa ggatctcaat ttggttttca gtatctgcat caactacgct actgtaaaaa
     4861 cgtatggtct cgcgcatcca ggttagctgg ttgccgagcc acatacgatt ataaataaag
     4921 gtaaccgcaa ctgacacggc aagtaccgca agcacgataa ccagcgtgtt aatcagattc
     4981 atgcggcgct tcatgcctca gcgcctgcct tatagcctat attacgtacc gttttaatca
     5041 aagagccctt tgagcccagt ttcttgcgca gtgaagcaac atgcacatcc acagtgcgtg
     5101 tgccaccttc aaagtcatag ccccaaacgc tttccagcag tttttcgcgc gaataggcca
     5161 tgccaggatg agaaaacaac tggaacaaga gttcaaactc tttaaaggtc aggtctaatt
     5221 cttccccatc aacgcgcgct gtacgattga gatagttaat ttcaatgccg ttgtactcaa
     5281 gatgctggat aattgtgctg ttgctgcgac gtttcaccgc acgaatacgt gctaaaagtt
     5341 ccatcacgct aaatggtttc gagatatagt catccgcgcc taaatcaagc ccgatgactt
     5401 tatcatattc cccgcccaag gcggttagca aaataaccgg aatttccttg gtatcaggat
     5461 cggctttgag ctttttcaaa agtgccaaac cactctcgcc gggcaacata atatccaaaa
     5521 tgatgagttc gggcgttttt tcgctgatga cctcgagcaa tgtggatcca tcctcaaaaa
     5581 gtcttgtttc gtagccttct ttttgaaggg cataggcaag caattcccgg atggattggt
     5641 catcttcaat acagtaaatc atgagctact cctaatgttg ctcttgctgt tctgggtcgc
     5701 ctttgatagc aaagataacc cagcgcgcaa tgcgttctgt attatcgcca atacgctcaa
     5761 gatatttggt aatttgcatc gcgtcaataa gatatttgta gctgtcagta tcggttctga
     5821 tgcggtctgc aatttcgtcc atggttttat caaaaagtgc gtcaacttcg ttatcgctgg
     5881 caatcacttc ctctgccagt tcaaggtcat tttcaacgaa ggaatccaaa gccttcttaa
     5941 gcattaccat aaccctgcta ttcatatcga taagttgatt aacagcaaac aagttattaa
     6001 taggaccaac gaaatctaaa agctctacaa tttcaataac atgtccgcca atgcgttgta
     6061 agttattcgc gatttttagc accgaagaaa taaagcgtaa atccttggca acaggttgct
     6121 gggtgctcat aattcgcaaa caatcattct caatgcgacg ctcttttgag cgaatggtcg
     6181 actcttcgtc caccatcaat ttggcttctt catagtcgcc ttcaacaaaa gctttttgca
     6241 aagtacgaca cattccttca atatacgagc cgtacagaat gacttctgtt ttcagctctt
     6301 caagttcttt aaaatatttt gctctcatca tttaccacct atccaaaacg acctgtaatg
     6361 taattttcag tgcgttcatc tttcggatat gagaatatct gtccagtttc accggtttcg
     6421 ataatttcac cagtcaagaa aaacgctgtt ttatccgaaa ttctcgcagc ttgttgcata
     6481 ttatgggtta ctataacaat agtatacttt tctttgagct caattaccaa atcttcaatt
     6541 ttacctgttg agattgggtc aagcgctgaa gtcggttcgt ccatcagcag gatttcaggt
     6601 tctactgcca atgcgcgcgc gatacaaaga cgctgttgct gaccaccgga aatttgtagc
     6661 gcgtttttcc tgagttcgtc tttcagctca tcccaaacag cggctttgcg caaactgtct
     6721 tcaacaattt cgtccagtct ggctcgattg cgcaaaccat gtacgcgcgg accgtatgca
     6781 atattatcat agatactaat cggaaagggg ttaggtgttt ggaagaccat accaactctt
     6841 ttacgcaagg cattcacatc cacaccaggc gcataaatat cttcgccgtc aatgagaatt
     6901 tttcctgtaa tcttgcagtt ttcgattagg tcgttcattc ggttgagcga ttttatcaat
     6961 gtggatttac cacaaccgct cggaccaatg aatgccataa tctcgttggc agggatatca
     7021 aggttagcgt tttttatggc ctggaagtcg ccataaaaga gatccatgtt ttcaatctta
     7081 atttttatat ctgtcatttt gttttattta ccagtttact aagtcttgtt gaaagcctgt
     7141 tgatgatgag aacaattacg agcaaaacca acgctgtcgc aaaggcttcg cgaacgtgga
     7201 atgcttcaga agaaagcaca tacatatgca aggcaagtgt tctaccggat tctgtcaggc
     7261 gtgtgggtaa ttgcgccatg gtacctaaag taaagatgaa ggctgcggtt tcgccaacga
     7321 tacgtccaat ggaaaggatg acgcccgcca aaatacccgg catggccact ggcaaacaga
     7381 ccttaacgat tgtttgcagt ttggtagcgc ccagtcccag actggcttgc ttaagcaagg
     7441 ggtcaacggc aagcagagct tcttccgttg tacggataat caagggcaaa accataatgg
     7501 ctgctgtaaa cgcgccagca atcaaagaat aggaccactg caagcggaac acaaagaaaa
     7561 gcataccgaa caaaccgtat acaatactgg gaattcccgc cagggtctcc gttgccagtc
     7621 gaatggcaat aaccacttta ctttgctgcc tggcatactc ggtgagataa ataccggtta
     7681 acacgccaaa tggaattgcg attatcaaaa ccaggataat caacatgatt gttgtaatca
     7741 tggctggcat cagcgaaaga ttttcgctgg tgtatttcca ggcaaacatt ttccatgaaa
     7801 gaccgggaat accacggata gcgatatatc ccacaacaat caggaagata gctaccgtga
     7861 gtattgcggc aaatttaacc aaaaaacgca aaactttact catggcggtc ccttcgtttt
     7921 accaggaaaa agaggaaatt gattaccagg atgaagatga agagcaccat cgcggtggca
     7981 ataagtgcct gacggtgttc aacacccgca tatgccatct caagtacgat gttggtagta
     8041 agcgtgcgca cgccactggt cagacttttt ggcaaacgtg gctggttacc ggcaaccata
     8101 taaaccgcca tcgtctcgcc aatcgcgcgc ccaatgccca aaatg
//
LOCUS       DAOSFC010000022         8091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7634_length_8091_cov_5.046665, whole genome shotgun sequence.
ACCESSION   DAOSFC010000022 DAOSFC010000000
VERSION     DAOSFC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7634_length_8091_cov_5.046665"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PK791_01200"
     CDS             <1..363
                     /locus_tag="PK791_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH91963.1"
                     /translation="SDQQKLVSELSGGERNRVHLARTLSTEANVLLLDEPTNDLDVNT
                     LRALEAALEDFAGTAIVVSHDRWFLDRIATHILELGHEDAPKLYLGNWSDYEQYQLSL
                     GREMGINRRDRMRSLKRD"
     gene            complement(431..646)
                     /locus_tag="PK791_01205"
     CDS             complement(431..646)
                     /locus_tag="PK791_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOH91964.1"
                     /translation="MSEREKGTVKWFNGEKGFGFIAPDSGAKDVFVHYSSIQVDGYRT
                     LKEDQKVEFTIVQGEKGPQAQDVKVIA"
     gene            complement(820..3123)
                     /gene="gltA"
                     /locus_tag="PK791_01210"
     CDS             complement(820..3123)
                     /gene="gltA"
                     /locus_tag="PK791_01210"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOH91965.1"
                     /translation="MDTKKTLRSKKSTIELNTIIKKETLAERIDRYIVYVPHIARKAQ
                     PGQFVIVRLSDQGERVPLTICDKDPEKGTITLVVQAVGKSTNMMAEMHDGERLKDVLG
                     PLGNASEIKNFGNVVVIGGGLGAAVSFPVAKALKAAGNHVTSIVGARTKDLLILEDEI
                     REFSDELHITTDDGSYGFHGFATNMLQDLIDAGQHFDRAIAVGPLPMMRAITQVTKPH
                     SIPTIVSLNTIMVDGTGMCGGCRVLVGGKVRFTCVEGPEFDGHEVDFDTIIYRNRAYN
                     HDEDCRLDTAIATLEKPNLEDYPLAGEKERQQMPELPIETRIHNFEEVALGFTPEQAL
                     AEANRCLDCRVPKCVEGCPVSVKIPDFIRLIKSGDPIAAGKLIKEDNSLPRVCGRVCP
                     QADQCEGNCVLGKRGQPIAIGALERYATDLLAKEDASIQIESKATNTLPPVAIVGSGP
                     ASLSCAGDLARAGYPVTVYEAFHDFGGVLRYGIPEFRLPKEIVSNEVETLAKMGVRFE
                     ANVLIGATHTIQDLQNEMGYKAVFIGSGAGLPRFLNIPGESMIGIYTANEFLTRVNLM
                     KAYTYPKVDTPILNMHEKTVAVFGGGNTALDSARVSLRLGAKKVYIIYRRSEEELPGR
                     KEEIEHAMAEGVEFTFLRNPQEFYGNSQGWLTGVRLVKMELGEPDKSGRRSPKAIEGS
                     DYILHIDRAIVAIGNGSNPIIQKNTPGLDVNIQGHITVNPETMQTSLPGVFAGGDIVT
                     GGATVILAMGAGRKAAKSIMDYLGNGS"
     gene            complement(3155..4072)
                     /locus_tag="PK791_01215"
     CDS             complement(3155..4072)
                     /locus_tag="PK791_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91966.1"
                     /translation="MGNSLQQGIEAAKAGKLGLALEHLKNAVVEEPKNPEVWVWLAGI
                     IDDEEKQRVFLNKALELDPDNVAAQRGIAYLQRQKYVPSQSSVTENKLATEKSPQSED
                     EPDLEYPDPNDLTMHVETFAKQVDAIGDPQAPSQAQIVRESKTLKTKPKRSIVDIIIY
                     GLTLIVFTIIGILIGATIKNRNIIPPEPTEQAQISPQSPGDGVFLSINNVFYKMALGQ
                     NEPPADDGIPSIMARLPKIVVNNALLIPTKLKLLDENNNDISFTVEENPDTSFTISPT
                     SGLAPGQYCLLHPISDENQAFYWCFKVLE"
     gene            4166..4660
                     /locus_tag="PK791_01220"
     CDS             4166..4660
                     /locus_tag="PK791_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH91967.1"
                     /translation="MKIGILSDSHNEIENVRKAVSVFESLGIDTVIHCGDVTIPRVLE
                     ALKSFRVFLAYGNMDIDKEMLSITLKKPDKQNKVGPFLDFELAGKRILVIHGDDGYGL
                     RMAIKSGEYDYVFTGHTHRVRDEKVGNTRVINPGALGGAFYEPRSIAYLDLEIDKLGR
                     YYLQ"
     gene            4758..4832
                     /locus_tag="PK791_01225"
     tRNA            4758..4832
                     /locus_tag="PK791_01225"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4793..4795,aa:Pro,seq:ggg)
     gene            complement(4900..5643)
                     /locus_tag="PK791_01230"
     CDS             complement(4900..5643)
                     /locus_tag="PK791_01230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH91968.1"
                     /translation="MDNAFWHQRFILQSLWTKPLRNHLYKKLNLHAHSKILELGSGTG
                     ALLEENLTISPYTFGLDFDHARTIFAKQNFPDALLTTANAYHLPFPTGSFDLSFCHYL
                     LLWIDNPVDVLKEMRRVTRPGGTVIAFAEPDYHARIDTPQIFQEIAVLQNESLQNQGV
                     RLDTGRRLMQYFTNAGFSDVSMGMLSGEWNSKQTTKFELEWEIIAHDLQEVLPPEKIN
                     ELKLKAFESYARGGAISFIPTFYAWASVE"
     gene            complement(5636..6745)
                     /locus_tag="PK791_01235"
     CDS             complement(5636..6745)
                     /locus_tag="PK791_01235"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOH91969.1"
                     /translation="MSKFTDFFKFSIKDVQEIEPGFYTSHDLMHGETPYRLHLRVEPN
                     HQGLLTLNASTVLHLNKTAMEMAYFLIKGQNPSEIAKSISQRYKAPKDEIKRDVDSFY
                     ETLTGFIFHEDLPPTTILGQIENPEQSDIVAPYRLDCYFSPTTERSILNTAEWKIVFE
                     KAFNAGIPHVMLVDIHDALVDELPDLLRHIEELGLVSGLVTDLSELKNDAFIDEILAC
                     GLDHLLIDANPRDQENLAQILHILSKDIFTCLRMPFDADFDYSKIMQELFAQGINAFA
                     FSNIQDELDINFLELQALISEHGISIADDMPQSRLADELRIPFPTNNKINLKYLALLP
                     NGDLLIPSDKPITIGNLLQEDWNILWAKCQDYSHG"
     gene            complement(6758..>8091)
                     /locus_tag="PK791_01240"
     CDS             complement(6758..>8091)
                     /locus_tag="PK791_01240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH91970.1"
                     /translation="FPPKPGIYKFDHQENGGKSRVHLRLEDNHSGVLVVNATRMIHLN
                     PTAGLMAWQYLNKTPETEAIKQLTTRFNISRKDAKQDYEETTSKIALLISDNSNTCPV
                     CDLGLESIAPFSSQLSAPYRMDLALTYRCNNNCSHCYNARERNYPELNTERWKQIIDK
                     LWDIGIPHLVFTGGEPTLRDDLAELVRYAEQKGFVTGLNTNGRKLANPAFLQSLIDAG
                     LDHVQITLESHIASVHDEMVLAKGAWEETVQGIKNVVNSKLYFMTNTTLLKNNAGNLI
                     EHLLFSESLGIPTIGLNALIYSGRGNTVNTGLSETELPALLKIAIEHTNRSGQRLIWY
                     TPTQYCHFDPMQLELGIKGCTAAYYNMCIEPDGQVIPCQSYYEPLGDLLDHTWHEIWN
                     HPLAINLRERKSAPQACRHCDYFQECGGGCPLSRDRQQVQPIFKDLILPTS"
BASE COUNT         2073 a         1654 c         1979 g         2385 t
ORIGIN      
        1 tctgatcaac aaaagttagt cagtgagtta agtggtgggg agcgaaatcg ggtgcatttg
       61 gcgagaactt tgagcacaga agcgaatgta ttacttctcg atgaaccaac aaacgacttg
      121 gatgtgaaca ctttgcgcgc acttgaagca gcgcttgaag atttcgcggg aaccgcaata
      181 gttgtcagcc atgaccgctg gtttttagac cgcatcgcaa cgcatatcct ggagttgggg
      241 catgaagatg cgccaaaact gtacttagga aactggtcgg attacgagca atatcagctt
      301 agtcttggcc gagagatggg tatcaaccga cgtgacagaa tgcgaagttt aaagagagac
      361 taattagatc caaaaccatt aaatttaaaa gccgtggctt gaccacggct ttgttgtcga
      421 cgttagaata ttaagcaata accttcacat cttgtgcctg gggccctttt tcgccttgca
      481 caatcgtaaa ttcaaccttt tggtcttctt tcaaggttct gtatccatcg acttgaatag
      541 agctgtaatg gacgaaaaca tctttcgcgc cactatctgg ggcaatgaat ccaaagcctt
      601 tttcaccatt gaaccactta acggttcctt tttcacgttc tgacattcta aaaaaatcct
      661 ctcaatactg aaattgtttt gtgttactgg gggtttactt catgagatac gcgcagatat
      721 gcaaactgcc aatacgcgct taactttact acaatcctaa aattgcgtca aacaaaatca
      781 cttatatttg ctttggtgta agtttgcaaa acctggtctt taacttccgt tgccgagata
      841 atccatgata gattttgctg ctttgcgacc ggcacccatt gccaggatga ccgttgcgcc
      901 accggtaacg atatcacctc ccgcgaaaac gcctggcaga ctggtctgca tggtttcggg
      961 gttaacagtg atatgtccct ggatgttaac gtcaagtccg ggtgtattct tttggataat
     1021 ggggtttgaa ccattgccaa tggcaacaat ggcgcggtct atgtgcaaga tatagtcact
     1081 gccttcaatc gctttagggc ttcttctgcc ggatttatca ggttcgccga gttccatttt
     1141 caccaaacgc acgccggtga gccagccttg actgttccca taaaattcct gcggattgcg
     1201 taagaaggta aactcaacgc cttccgccat ggcatgttcg atttcttctt ttctgccagg
     1261 cagttcttct tcgctgcgac gataaatgat atagactttt ttggcaccaa gtctaaggga
     1321 tacccgtgcg gaatcaaggg cagtatttcc accaccaaag acggcaacag ttttctcatg
     1381 catattaaga atcggtgtgt caaccttggg ataggtgtag gctttcataa ggttaacgcg
     1441 ggtgagaaac tcgttggcag tgtagatgcc aatcatcgat tcaccgggga tattcaaaaa
     1501 acgaggtaag ccggcaccgg aaccgataaa gacagctttg tagcccattt cattttgcag
     1561 gtcctggatg gtgtgggtgg cgccaatcaa aacgttggct tcaaatcgaa cgcccatctt
     1621 cgcgagtgtt tctacttcgt tgctgacgat ttcttttggc agacgaaact caggaatgcc
     1681 atagcgcaat acaccaccaa agtcatgaaa tgcctcataa acggtaacag ggtagcccgc
     1741 gcgtgccaaa tcgcccgcgc aactcaggct tgctggcccg gatccaacga tggcaaccgg
     1801 tggcaatgtg ttcgttgctt tgctttctat ctgaattgac gcgtcttctt tcgcaagcag
     1861 gtctgttgca taacgttcaa gtgcgccaat agcgatcggt tgaccacgtt ttccaagaac
     1921 gcaattacct tcgcattggt cagcttgcgg acagacacgc ccacaaaccc gtggcaagga
     1981 gttgtcttcc ttgatgagtt tgccggctgc gattgggtca ccagatttga ttagtcgaat
     2041 aaaatcgggg attttaacgg ataccgggca gccttcaaca catttaggca cacggcagtc
     2101 caaacagcga tttgcttcgg ctaaagcctg ctctggggtg aaacccaagg caacttcttc
     2161 aaagttgtgg atgcgtgttt caatgggtaa ttcgggcatc tgttggcgtt ctttttcgcc
     2221 ggcaagtgga tagtcttcga gattaggttt ttcaagcgtg gcgattgctg tatcaaggcg
     2281 gcaatcttca tcatggttat aagcgcggtt acgatagatg attgtgtcaa aatcgacttc
     2341 atggccatca aattccgggc cttcaacaca ggtgaaacgg actttgccac caaccaacac
     2401 cctgcatccg ccgcacatgc cagtgccatc aaccataatg gtattaagag aaacaatggt
     2461 ggggatacta tggggtttgg ttacctgagt tatggcgcgc atcattggta gcgggccgac
     2521 tgcaatcgcg cggtcaaagt gctgaccggc gtcgattaaa tcttgcagca tgttcgtagc
     2581 gaacccatgg aagccataac tgccatcatc ggtggtaatg tgaagttcat cagaaaattc
     2641 gcgaatttcg tcttcaagga tgagtaaatc tttggtgcgc gcacccacaa tggaggtcac
     2701 atgattgccc gcagctttga gagctttggc aaccggaaac gatacggctg cgcctaatcc
     2761 acccccgata acgacaacat taccaaagtt cttgatttct gaagcattac ccagtgggcc
     2821 taaaacatct tttaagcgct caccgtcgtg catttctgcc atcatgttcg tacttttgcc
     2881 tactgcctgc acaaccaggg taatggtgcc tttttcgggg tccttgtcgc agatggtcaa
     2941 aggcacccgt tcgccttggt cagaaagtcg gacgatgaca aactgtccgg gttgggcttt
     3001 cctggcgatg tgcggcacgt agacgatgta gcggtcgatg cgttcagcca gggtttcttt
     3061 tttgatgatt gtgtttagtt caatagtgct ttttttactg cgtaaggtct tctttgtatc
     3121 caaggctcag ctcctcatct gccatgaaat gcgattattc aagaactttg aaacaccagt
     3181 aaaaagcctg gttttcatct gaaatagggt gtaacaagca atattgccca ggcgctaagc
     3241 cagatgtggg agagatggta aaggaagtgt ctgggttttc ttcaactgtg aaagaaatat
     3301 cgttattgtt ttcatccagc aattttagtt ttgtgggaat aagtaatgcg ttatttacaa
     3361 cgatttttgg cagccgtgcc attatggaag gtatgccatc atcggcaggc ggttcgtttt
     3421 gtccaagcgc cattttgtaa aagacattat taatgctcag gaaaacgcca tcgccagggg
     3481 attgcgggga tatctgtgct tgttctgtag gctctggtgg gatgatgttg cggtttttga
     3541 ttgtagcgcc gataagaata cctatgattg taaaaacgat aagggttaaa ccgtaaatta
     3601 taatatcgac aattgatctt ttaggtttgg ttttgagcgt tttgctttcg cgaacgattt
     3661 gtgcctgaga tggtgcctgt gggtcgccta tagcgtcaac ttgtttggca aatgtttcaa
     3721 catgcatggt gagatcattc ggatcggggt attccaggtc gggttcatct tcagattgag
     3781 gagatttttc ggtggctagt ttattttcgg taactgagct ttggcttggc acgtattttt
     3841 gacgttgcaa ataggcgata ccccgttggg ctgctacgtt atctggatca agttcaagcg
     3901 ctttattaag aaagacgcgt tgtttttctt catcatcaat aattcccgct aaccataccc
     3961 aaacttcggg gtttttgggc tcttcaacaa cagcgttctt gaggtgttca agcgctaaac
     4021 ccagttttcc ggcttttgcc gcttcaattc cttgctgtaa agaatttccc atagtttggt
     4081 cctttggaaa agtgattgaa tctaatgaaa ttttaccaca atggtttaag catggttata
     4141 attggcgtct cagaaaggga ttgatatgaa aattggcatt cttagcgatt cacacaatga
     4201 aattgaaaat gtaagaaagg ctgtatcggt ttttgaatcc ttggggattg atacggttat
     4261 tcattgtggt gatgtgacca taccgcgagt tttggaagcg ttaaagtctt ttcgtgtttt
     4321 tctggcttat ggaaatatgg atattgacaa ggaaatgcta tcaattacat tgaagaaacc
     4381 agacaagcaa aacaaagtgg gaccatttct ggattttgaa ttagctggaa aacggatact
     4441 tgtgatccat ggtgatgacg gttatggttt acgaatggca ataaaaagtg gtgagtatga
     4501 ctatgtgttt actgggcata cacaccgggt tagggacgaa aaggttggta atacacgggt
     4561 gattaatcca ggcgctttgg gtggggcatt ttatgagcca cgatcaatag catatctgga
     4621 tttagagatt gataagttgg gacgttatta tctacagtag ttgcatgttt gttttaaaat
     4681 atttacgtaa gacaaattga aatggaaaat gcacttgcat ttaattttga atgagcttat
     4741 aatcgtggtc tgttgtacgg ggcgtggcgc agtccggata gcgtgcttgc atggggtgca
     4801 agaggtcgga ggttcgaatc ctctcgcccc gacaccaaaa ccatctgagt aatcaggtgg
     4861 ttttttcttt aaagagttga cttttgttag aaaataaatc tattcgacgc ttgcccaggc
     4921 gtaaaatgta gggataaagc tgattgctcc gcctcgtgcg tagctttcaa aggcttttag
     4981 tttgagttcg ttgatttttt ctggtggcag aacttcttgc aaatcatggg caattatttc
     5041 ccactcaagc tcaaattttg ttgtctgctt tgaattccac tcaccggaaa gcatgcccat
     5101 gctgacgtcg ctaaaaccag cgtttgtgaa gtattgcatt aaacgtctgc cggtgtctaa
     5161 gcggacacct tgattttgca aagactcgtt ttgtaaaacg gcaatttcct gaaagatttg
     5221 tggtgtgtca attcgggcat ggtaatccgg ctcggcaaag gcgatgacgg ttccgccagg
     5281 tcgcgtgaca cgacgcattt cttttagaac atcaacggga ttatcaatcc acaggagtaa
     5341 atagtgacaa aaagacaaat cgaagctgcc ggtgggaaaa ggaagatgat aagcgttcgc
     5401 ggtggtgagt agagcatcgg gaaagttttg ttttgcgaat atagttctgg catgatcaaa
     5461 atctaatcca aaagtgtatg gcgatatggt taggttttct tctaaaagcg cgccggtacc
     5521 actgccaagt tccaaaattt tggaatgggc atgcagattt agttttttgt aaagatgatt
     5581 gcggagtggt tttgtccaca aactttgaag gataaaacgt tgatgccaaa aagcgttatc
     5641 catgactata atcctggcac ttagcccaaa gaatattcca gtcttcttga agcagattgc
     5701 cgatggtgat tggtttgtct gatggtatga gcaaatcgcc attagggaga agggctaaat
     5761 acttcaggtt tattttatta ttagtcggga aaggaatcct gagttcatct gccaggcgtg
     5821 attgaggcat atcgtcagct attgagatgc catgttcgga gataagcgcc tgtaattcga
     5881 gaaaattgat gtctaattca tcttgtatat tgctgaaggc aaaagcgttg ataccctgcg
     5941 caaacaattc ttgcatgatt ttgctatagt cgaaatctgc atcaaagggc atccgtaaac
     6001 aggtaaatat gtccttgctg agaatatgca atatttgggc gagattttcc tggtcgcgtg
     6061 ggtttgcgtc gataagcaga tggtcaagtc cacatgccag gatttcatct ataaaggcat
     6121 cattttttag ctcagacagg tctgtaacca gtccgctgac caggcctaat tcttcaatgt
     6181 gtcttaacaa gtcgggcaac tcatctacta acgcatcatg gatgtccaca agcatcacat
     6241 ggggaatacc agcgttaaat gctttttcaa atacgatttt ccattccgcg gtattcaata
     6301 ttgacctttc tgttgtgggg ctaaagtagc aatccaggcg gtaaggcgca acgatatcgc
     6361 tttgttctgg attttcgatt tgacccaaaa tcgtggttgg gggaaggtct tcgtgaaaaa
     6421 taaaaccagt cagcgtttca taaaagctat ccacatctct tttgatttca tctttggggg
     6481 ctttatatcg ctgactaatg cttttggcaa tctcggacgg attttgtccc ttgatgagaa
     6541 agtaggccat ttccatcgct gttttgttga gatgcaaaac ggttgaagcg tttaatgtaa
     6601 gcaggccttg atggttaggt tcaacgcgca ggtgcaggcg atagggtgtc tcgccatgca
     6661 tcaggtcgtg gcttgtgtaa aatccaggtt caatttcctg aacgtctttt attgaaaact
     6721 tgaaaaaatc agtaaatttt gacatgctcg ctccttatta agatgtagga agaataaggt
     6781 ctttgaaaat gggctgtacc tgttgacggt cgcgacttaa aggacaaccg ccgccgcatt
     6841 cctgaaaata gtcacaatgt cggcaggctt gtggggccga ttttctctcg cggagattaa
     6901 tggctaatgg atggttccaa atctcatgcc aagtatggtc taaaagatct cccaggggct
     6961 cgtaatagct ttggcaaggg ataacttgtc catctggttc aatgcacatg ttgtaataag
     7021 cggcagtgca acctttgatg ccaagctcga gttgcatggg gtcaaaatgg caatattgcg
     7081 ttggcgtata ccaaataaga cgctgtccgc ttctgttagt gtgttcgatg gctattttaa
     7141 gtagtgccgg aagttcagtt tcggatagtc cggtgttgac cgtgttgcct ctgccggaat
     7201 aaatgagcgc attaagaccg attgtgggga tacctaaact ttcggaaaac agcagatgtt
     7261 caatcaaatt acctgcgttg tttttcaata atgtggtgtt ggtcatgaaa taaagctttg
     7321 agttgaccac attttttatc ccttgaacgg tttcttccca ggcacctttg gcaagcacca
     7381 tttcatcatg cacgctggcg atatgtgatt ccaaggtaat ttgcacatgg tctaaaccag
     7441 cgtcaattag actttgaagg aatgcggggt tggctaattt tcgtccgttg gtgttcagtc
     7501 cggttacaaa tcctttttgt tccgcatagc gtaccagttc ggccaggtca tcgcgtaagg
     7561 ttggctctcc accggtaaaa acaagatggg gtatgccgat atcccagagt ttgtcgatga
     7621 tttgtttcca tcgctccgta ttaagttcgg gatagttacg ctcgcgggcg ttatagcagt
     7681 gtgaacagtt gttgttacac cgatatgtaa gcgctaaatc catgcgataa ggcgcgctga
     7741 gttgacttga aaatggcgcg atgctttcca agcccaggtc gcatacgggg catgtatttg
     7801 aattatcaga gattagcaaa gcaattttgg acgtggtttc ttcgtagtct tgttttgcat
     7861 cctttcggct gatattaaaa cgtgtggtga gttgttttat ggcttcggtt tctggggttt
     7921 tgtttagata ttgccatgcc attaaaccag cagtaggatt tagatgaatc atcctggtcg
     7981 catttacaac caaaacacca ctatggttat cttcaagccg aagatgaacg cgcgatttac
     8041 ctccattttc ctggtggtca aacttataga taccaggttt tggtggaaag g
//
LOCUS       DAOSFC010000023         8059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7674_length_8059_cov_4.701649, whole genome shotgun sequence.
ACCESSION   DAOSFC010000023 DAOSFC010000000
VERSION     DAOSFC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7674_length_8059_cov_4.701649"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1353)
                     /gene="hflX"
                     /locus_tag="PK791_01245"
     CDS             complement(40..1353)
                     /gene="hflX"
                     /locus_tag="PK791_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOH91971.1"
                     /translation="MTDLFYTEEELEKAIMVGVWLGKNNSAVLDLEGSLEELAFLAET
                     AGIEVVGEISQNLINPNPKTYIGSGKVEELKLLTDELGADMVLFDDELSPRHQRELEE
                     VFGNKVKVIDRTALILDIFAQHADTAEGKLQVELAQLHYRLPRLTRAWTHLARQAGGG
                     GGRTGSVGGVGLRGPGETQLETDRREIQKRITKLEDELEKVRERRARHRSQRKKSNIP
                     IVALVGYTNAGKSTLLNAISSAEIYVADQLFATLDPTIRQLQLPGGTEVLVSDTVGFI
                     QKLPTQLVAAFRATLEEINEADLLLHIVDASHEHYLEQSYSVLETLREIDAGEIPILT
                     VYNKIDLLDAETLDDLEQDAGQDTYFISAKTGEGIEALQEAIADELFTGQSMVKVLLP
                     YSEGKLVSIFHERGNVINIEHLEEGILIYGRLPKETLPYFREYAI"
     gene            1474..2886
                     /locus_tag="PK791_01250"
     CDS             1474..2886
                     /locus_tag="PK791_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91972.1"
                     /translation="MLQIPHQQITTTTSAHLAQTMTLLSLTGGELQEKIESELASNPA
                     LELVEERRCPMCKRLLPETGACPICSQPKSYDSNEPIVFISPRESFYTGTTGSTEQIP
                     EDQYVSQSVDLSTYVLRQVAAELAVEDRLITAYILNHLDEDGFLTTTVMEIARYHHRL
                     PSEVTEIIEMLKRCDPVGVCSSTPQEAMLAQIDALAETVKIPEHTREVVQNFLHKLSQ
                     QNYAEIAKKLGTTSQHIRKISNFITENLNPFPGRAHWGDVRNPSPIAPDVFHQPDVLI
                     YYLNENPKNPLVVEIILPVGGTLRVNPLFKSSMKQADKDKTDEWRSDIEKASMLIKCI
                     QQRSNALRLLLEKIIVYQRDYILHGDKAMQPLTRAKLAKELDVHESTISRAVSTKTVQ
                     LPNKRIVPMAIFFDRSLAYRAEIKSIIEEEETPYSDSEIQALLAEKGINIARRTVAKY
                     RSLEGILPAHLRQKHGHQKA"
     gene            2891..4861
                     /locus_tag="PK791_01255"
     CDS             2891..4861
                     /locus_tag="PK791_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH91973.1"
                     /translation="MQQTKLPFQAVSYLSELDFLQQSKILELLPVATVIYKRDTDALL
                     YANQKFYELTRFSEDELFGMQLSDIIPYETDTNITSTDARPTAIRIANGDILQTAMRV
                     TSINLTNTIVLLAFYPPDFEVTEQKEPPEFHIPLMMDHFTMLSQLGEQNSSKSLLFAT
                     ASIVKSVLNAPITALYLSQESENGAFTKANLPETFNCQQLPQKISIGTGQLPVYDFLW
                     LTDSVPQHMLHEFTLAKDYSYAVILPVRDENHCHAVIYATGITPIPTVETMRFLSLLA
                     NQSVSILAKIHALENATKRLNTMRHIVQIERGITENMNEGLIVLTPDLRIAEMNTAAE
                     IILGYASKEVFLEDIEMVLIGNENLATHYKSAQQGISTIAGTNLMLNDRRGNAFPAQL
                     TCVPIILDGEVKSIIILLRDLSQTEQLLQHSKQLEQRAFLGEVSAVFAHEVKNPINSI
                     STGLQFIGMNMKPEDPHYNLVMRLQQDCQRLTHLTDSTLTFAKPVEYQPLPMNISALL
                     ENILERWAPRMTRLNINYLFESEPKNAIVLADGRAIEQVFVNLISNSIQAMDSEGGTL
                     SIQISPAQDSQPPQYEITVADSGPGIPDDIIKHIFEPFQTTKTTGTGLGLAITKRIVT
                     AHKGYIFVESFPGGTMFRVLLPMSDIKEKNDN"
     gene            4851..6032
                     /locus_tag="PK791_01260"
     CDS             4851..6032
                     /locus_tag="PK791_01260"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOH91974.1"
                     /translation="MTTSILVVDDEETARMVISEFLRSKGYEVLEAGTLGDARIIVKE
                     GKADVIILDVQLPDGYGPHLLDEVARLPMRPQVIIITAHGEVSMAVDAMKAGAQDFIE
                     KPLDLIKLEAAVKRANEVISMRRELNHWRTSLGKNLEFIVGSSPQMAHVVELARRASE
                     MSVSALITGPTGTGKEVLARFIHQSGPRAGKPFIDINCPAIQPTMFESELFGHEAGAF
                     TGATQRRIGLMEVANDGILFLDEISSMPMDLQAKFLRALEERAFRRVGGTKLIDVDVQ
                     IIAASNRNLREMIEKHEFREDLYYRLKVVDLDLPTLAERKDDIPELVGYFVRRFNMRQ
                     GSNILDVSPRAMEAMMNYNWPGNIRELSNTIERAVLFCDDPVIDINHLPLEVVNYKKK
                     P"
     gene            6096..6428
                     /locus_tag="PK791_01265"
     CDS             6096..6428
                     /locus_tag="PK791_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91975.1"
                     /translation="MLEDNVQIIITIAGVIFLLGIVCLVISIIILARQAMNRNIRTIA
                     ESTAKLAQKGLTDGVSGLVGNASMLLSSLNDLTQSNTGIGIFFVFLSLVLLAVAYFLL
                     KPILFVAP"
     gene            6444..>8059
                     /locus_tag="PK791_01270"
     CDS             6444..>8059
                     /locus_tag="PK791_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91976.1"
                     /translation="MAIQWFLNHLNETFETNINRKIIQAYRQDPLVWKAIATIEHPED
                     WIALAGNSLFNWQVSKFALFTLRPDLLNEDYRDLSIDLPEELQHKSERLLETIRTTGL
                     EPGNLSDAIYLALALRNFRLEHDGWFELADFLGTVNKRADVWKTAFVILPAFVPDLED
                     AFIALIDNTEPESIDAITTLILHTVNTLFLDENQRYQLYTTLATDASIDFQLSLLKML
                     PNYETQAFIRLLASHLYDLTPESDETKSDFEIINLNRKRALLEQYAGLSDESAETIQS
                     VQQDFQTLRASFLNQLAKDLEKTQAEEARKIWEEVLSLDPDNEHYISEYAEFLIYEGD
                     VHQALYLLNTLSDRSIIKLYAWRYPAFAAISPESEYISDADLSSNLLSSPPSRFLASA
                     DKFHAARVAFESKNYTLALEFINKALLENPNHLDSIKLASKINQHLANLQDAIQNLAL
                     LTFIENDNLEHKRELTQLYLQAHEPQKALDTFAEIINASTHPSRDDSLYYAQLSITAE
                     QAEIAIPIARNFLAQDPLDGEAMVILAEAYL"
BASE COUNT         2228 a         2223 c         1639 g         1969 t
ORIGIN      
        1 aatcgttgta gctaatggca gcaacatcaa acgtaaaacc tatatggcat actccctgaa
       61 ataaggcaaa gtctccttag gcaatcgtcc gtaaatcaat ataccttctt ccaaatgctc
      121 tatgttaatc acatttccgc gttcatggaa aattgaaacg agttttccct cagaatatgg
      181 caataaaacc ttcaccatgg actgccccgt aaagagttca tcagcaatcg cttcctgcaa
      241 agcttcaata ccttcaccgg tttttgcgga gatgaaatag gtatcctgcc ccgcatcttg
      301 ttccaaatca tctaacgttt ccgcatccaa aaggtcaatt ttgttgtaaa ccgtcaggat
      361 gggtatctct ccggcatcga tttcccgcag tgtttccaaa accgaataac tttgttccaa
      421 gtaatgctcg tgcgatgcat ccacaatgtg aagcagcaaa tcagcctcgt taatttcttc
      481 cagcgtggca cgaaaagctg ctacaagctg tgtggggagt ttttgaatga agcccaccgt
      541 atcagacacc agtacctccg ttccgcccgg caactgaagc tggcgaatgg ttgggtcaag
      601 cgtggcaaaa agttggtcag ccacataaat ctccgcgctg cttatcgcgt taagtagcgt
      661 cgattttccc gcgttggtat atccgaccaa agccacaatc ggaatattgc ttttcttgcg
      721 ttgactgcga tgtcgggcac ggcgttcgcg tactttttcc agttcatctt ccagctttgt
      781 aattcgtttt tggatttccc gtcggtccgt ttccagctgc gtttcaccag gcccgcgcaa
      841 acctacgccc cccacactgc ccgttcgtcc accgccaccg ccggcttgtc tggcaagatg
      901 cgtccaggcg cgtgtcaagc gtggcaggcg ataatgcaac tgcgccagct ccacctgcag
      961 tttaccttcg gccgtatcag cgtgttgcgc gaaaatatcc aaaatcaagg ctgtccggtc
     1021 aatcactttg accttattac caaagacttc ctccagttcg cgctgatggc gtggcgaaag
     1081 ctcgtcatca aaaagcacca tatcggcgcc aagttcatca gtcaaaagtt ttagttcttc
     1141 gactttacct gaaccgatat aagttttggg gttgggattg attaaatttt ggcttatctc
     1201 gccaaccact tctattcccg cggtttcagc taaaaaagcc agttcttcaa gcgaaccttc
     1261 caggtccagt acagctgagt tgtttttccc cagccaaacg ccgaccataa tcgctttttc
     1321 tagttcttct tctgtataaa ataaatctgt caaatgcttt cctttgttac ggacttaaaa
     1381 gacttacaat ctttactctt attatagcgt atttgacaga tttctttggt ataattcttg
     1441 cacaaaggcg actccgtttc aggaaggatt aagatgttac agatacccca tcaacaaatc
     1501 acaaccacaa cttctgcaca cctggcacag accatgactc tgcttagttt aaccggtggc
     1561 gaacttcagg aaaaaattga gtccgaactc gcctctaacc ccgcgttgga actggtggaa
     1621 gaacgacgct gtcccatgtg taaacgtctg ttgcctgaaa caggtgcctg ccctatttgc
     1681 agccagccaa aaagctacga ttccaacgaa ccgattgtgt tcatctcacc ccgcgaaagc
     1741 ttctatactg gcaccacagg aagcaccgaa caaatccctg aagaccaata cgtatcacaa
     1801 tccgttgacc tttccactta cgtcttgcgt caggttgccg cagaattagc cgttgaagac
     1861 cgcctgatta ccgcctacat tctcaaccat ctcgatgaag atggctttct caccacaacc
     1921 gtcatggaaa tcgctcgata tcatcatcgc ctgccatctg aagttaccga gattatcgaa
     1981 atgcttaaaa ggtgcgaccc cgtcggtgtt tgttcttcca ccccacaaga agcgatgctt
     2041 gcccaaattg acgctttagc agaaacagtt aaaattccag aacatacccg cgaagttgtt
     2101 caaaactttc tgcataagct cagtcagcaa aactatgcag aaattgccaa aaaactgggc
     2161 acgaccagtc agcacatccg aaagatatct aacttcatta ccgagaacct gaaccccttc
     2221 cctgggcgcg cgcactgggg agacgtgcgt aaccccagtc ctatagcacc ggatgtcttt
     2281 catcagcctg atgtgctcat ttactatctc aacgaaaacc caaagaaccc actcgtggtc
     2341 gaaatcatcc tgcctgttgg tggcacatta cgcgtcaatc cgttattcaa atcttcaatg
     2401 aaacaagctg acaaagacaa aaccgacgaa tggcgcagtg acatcgaaaa agcgtccatg
     2461 ctcatcaaat gcattcagca acgctcaaac gccttgcggc ttttgcttga aaaaatcatt
     2521 gtttaccaaa gagattacat tctccacggc gacaaagcca tgcagccact cacccgcgcc
     2581 aaactcgcca aagaactgga cgtgcacgaa tccaccattt cgcgcgcggt ctcgacaaaa
     2641 accgtgcagc tacccaacaa acgcatcgtg ccgatggcca tcttttttga ccgcagtctt
     2701 gcgtaccggg ctgaaataaa atcaatcatt gaagaagaag aaactcccta cagcgactct
     2761 gaaattcaag ctcttctcgc tgagaaaggg ataaatatcg cgcgcagaac cgtcgccaaa
     2821 tatcgctccc tggaaggcat tctgccagca catttgcgcc aaaaacacgg acatcaaaag
     2881 gcatagaact gtgcaacaaa caaagcttcc ttttcaagct gtttcttatc tgtcagaact
     2941 tgactttctt cagcaatcta aaattcttga acttctacca gtcgcgacgg taatctacaa
     3001 acgcgacaca gacgccctgc tgtatgccaa tcaaaagttt tatgaactca cacggttctc
     3061 agaagatgag ctttttggca tgcaactcag cgacattatt ccctacgaaa ccgacaccaa
     3121 catcaccagc accgatgccc gtccgacagc gattcgtatt gccaacggtg acattttgca
     3181 aaccgcgatg cgcgtgacgt ccatcaacct gaccaacacc atcgtgctcc tggcctttta
     3241 tccacccgat ttcgaagtca ccgaacaaaa agaaccacct gaatttcaca tacccttgat
     3301 gatggaccat ttcaccatgc tcagtcagct tggtgaacaa aactcgtcaa aaagtctgct
     3361 cttcgctacg gccagcattg taaagtctgt tctcaatgcc ccgattaccg cactttatct
     3421 atcacaagaa tcagaaaatg gcgcgtttac aaaagcaaac ctgccagaaa ccttcaactg
     3481 tcagcaatta ccccaaaaaa tcagcatagg aacaggtcag ctgcccgttt acgacttcct
     3541 ttggctaaca gacagcgtac cccaacatat gcttcacgag ttcacattag ccaaagacta
     3601 cagctatgcc gtcatcttgc ctgtccgtga tgaaaaccat tgtcatgcgg ttatctatgc
     3661 cacaggcatt actcccattc ccaccgtcga aacgatgcgc tttttgtccc tgctagccaa
     3721 tcaaagtgtg agtatcctgg caaaaatcca cgcgctcgaa aatgccacaa aacgcctgaa
     3781 caccatgcgc catattgtgc aaatcgaacg cggcatcaca gaaaacatga acgaaggact
     3841 tatcgttcta acccccgact tgcgcatcgc ggaaatgaac accgccgcgg aaatcatcct
     3901 gggctacgcc agcaaagaag tctttctgga agacatcgaa atggtgctca tcggcaatga
     3961 aaaccttgcc acgcactata aatctgccca acaaggcatc agcaccatcg ctggcacaaa
     4021 cctaatgctc aacgaccggc gcggaaatgc atttcccgct caactcactt gcgtgcccat
     4081 tatcctggat ggtgaagtca aaagcatcat cattttactg agagacctaa gccaaaccga
     4141 acagttgctt cagcacagca aacaactgga acaacgcgcg tttcttggcg aagtttccgc
     4201 cgtcttcgcc cacgaagtca aaaaccccat caacagcatt tccacgggtt tacagttcat
     4261 aggcatgaac atgaaacctg aagatccgca ttataatctt gtcatgcgct tacaacagga
     4321 ttgccagcgg ctaacccatc tcaccgattc aacgctcacc tttgccaaac cagtcgaata
     4381 tcagccctta cccatgaaca taagcgcgct cttggagaac atccttgaac gctgggcgcc
     4441 ccgcatgaca cgcttaaata tcaattatct ttttgaatcc gaacctaaaa acgcgattgt
     4501 cctggctgat ggacgtgcca ttgaacaggt ttttgtgaac cttatcagca acagtattca
     4561 ggcgatggat tcagaaggtg gcacgctaag tattcaaatc agccccgccc aggacagcca
     4621 gcccccgcaa tatgagatta ccgtcgctga ctctggccca ggcatccctg atgacattat
     4681 taaacatatt tttgaaccct tccaaaccac aaaaacaacc ggtaccggac ttggcctggc
     4741 catcaccaag cgcattgtga ccgcgcacaa aggctacatc tttgttgaaa gcttccccgg
     4801 tggcaccatg ttccgggttt tattgcccat gtcagacata aaggagaaaa atgacaacta
     4861 gtattttggt cgttgacgat gaagaaaccg cacgaatggt aataagcgaa tttttacgct
     4921 caaaaggcta cgaagtactt gaagccggca cccttggcga tgcccgtatt atcgtgaaag
     4981 aaggcaaagc cgacgtcatt atccttgacg tgcagctgcc agatggctat ggcccccacc
     5041 tgctcgacga agtcgcccgt ctacccatgc gtccgcaagt gatcatcatc accgcgcatg
     5101 gcgaagtgag catggcggtc gatgccatga aagccggcgc gcaggatttc atcgaaaaac
     5161 cccttgacct catcaagctt gaagccgctg taaaacgcgc caacgaagtc atcagtatgc
     5221 gccgtgaact aaaccactgg cgcacatccc tgggcaaaaa tctggaattt atcgtgggtt
     5281 catcgcctca aatggcgcat gttgtggaac tcgcccgtcg cgcttctgaa atgtccgttt
     5341 ccgcgcttat caccggccca actggcaccg gtaaagaagt tctcgcgcgc tttattcatc
     5401 aaagcggtcc gcgcgctggt aagcctttta ttgacatcaa ctgcccagcc attcaaccaa
     5461 ccatgttcga atcagaactt ttcggtcacg aagccggggc ctttaccggc gctacccaac
     5521 ggcgcatcgg tttgatggaa gtcgcgaatg atggcatctt gtttttggac gaaatctcct
     5581 caatgcccat ggaccttcag gcaaaatttt tacgcgcgct cgaagaacgg gctttccgtc
     5641 gcgttggtgg cacgaaatta atagacgtcg atgtgcaaat catcgctgcc agcaaccgca
     5701 acctgcgcga gatgattgaa aaacacgaat tccgcgaaga cctttattac cgcctcaagg
     5761 tggttgacct agacctgccc acactcgctg aacgtaaaga tgacatcccg gaacttgtcg
     5821 gctactttgt gcgtcgcttt aatatgcgcc agggctcgaa cattctcgac gtcagtccac
     5881 gtgccatgga agccatgatg aactacaact ggcccggcaa tattcgcgaa ctgagcaaca
     5941 ctatcgagag agcggtgctg ttctgcgacg acccggtcat tgacatcaat cacctgcctt
     6001 tggaagtcgt caactataag aaaaagccct aaaacagacc cggattggca tattagttgc
     6061 aacatagtaa ctgaaggact tatagaggag ttactatgtt agaagacaat gtccaaatca
     6121 tcattacgat tgctggggta atctttttac tgggtatcgt ttgcctggtt atcagcatca
     6181 tcattttggc aagacaggcc atgaacagaa acattcgcac cattgctgag tcaaccgcca
     6241 aactcgccca aaaaggtttg actgacggtg tttccggttt ggttggcaac gcctccatgt
     6301 tgttgagttc actgaatgat ctcacccagt caaacactgg catcggtatt ttctttgtgt
     6361 ttttaagcct ggtactgttg gctgttgcct attttctgct aaagccgatt ttgttcgtcg
     6421 cgccataatt caaggagggc aaaatggcta ttcaatggtt tttaaatcac cttaacgaaa
     6481 ccttcgagac aaacataaac cgcaagatta tccaggctta caggcaagac cccctggttt
     6541 ggaaagcgat tgcaacaatc gaacaccctg aagactggat tgccttagca ggcaatagtc
     6601 tttttaactg gcaagtatcc aaattcgcgc ttttcacctt gcgccctgat cttctcaatg
     6661 aagattaccg cgatttgtcc attgaccttc ccgaagaact gcaacacaaa agcgaacgac
     6721 tgctcgaaac cattcgcacg actggactgg aaccaggcaa cttgtccgac gctatttatc
     6781 ttgctttggc tttgcgaaac ttcaggctgg aacacgatgg ctggtttgaa ctggccgatt
     6841 tccttggcac ggtcaataaa cgtgctgatg tatggaaaac cgcttttgtc attcttcccg
     6901 cctttgttcc cgaccttgaa gatgctttca tcgcgctgat agacaacacc gagccagaaa
     6961 gcattgatgc catcacaacg ctgattttac acacggtaaa cacgctattc cttgacgaaa
     7021 accaacgata tcagctctac accacccttg ccactgacgc gagcattgac tttcaacttt
     7081 ccttgctcaa aatgctccca aattatgaaa cacaagcttt cattcgtctt ttagcaagtc
     7141 atttatacga cctaacccca gaaagtgatg aaaccaagtc agattttgaa ataatcaacc
     7201 ttaaccgcaa acgcgcgctt ttggaacaat acgctggatt aagtgatgaa tcagcagaaa
     7261 caattcaatc cgttcagcag gattttcaaa ccttacgggc atcattccta aatcagcttg
     7321 ctaaagattt ggaaaaaacg caagccgaag aagcgcgcaa aatctgggaa gaagtactca
     7381 gtcttgaccc cgacaatgaa cactacatca gcgagtacgc tgaatttctc atttacgaag
     7441 gcgacgtgca tcaggccttg tacttgctca acacgctgtc cgaccgttca atcataaaac
     7501 tttacgcctg gcgttaccct gcttttgctg caatttcgcc agaaagcgaa tatattagcg
     7561 atgcagacct aagcagcaat ctcctttcgt cgccaccatc gcgattttta gccagcgctg
     7621 acaaattcca tgctgcccgg gtcgcctttg aaagcaaaaa ttacacttta gcccttgaat
     7681 ttatcaacaa ggccctgctt gagaacccca accacctgga ctccattaaa ctggccagta
     7741 aaatcaacca gcatttggcg aatttacagg atgcgattca aaaccttgct ctccttacct
     7801 ttatcgaaaa tgataattta gaacacaaac gggaactcac ccaactctat ctccaggcgc
     7861 acgagcccca aaaagcgctc gatacttttg ctgaaattat taatgccagc acgcatccca
     7921 gccgagacga ctcgctttat tacgcacaac tgtccatcac cgccgaacaa gctgaaatcg
     7981 ccatcccgat tgcgcgtaat ttcctggcgc aagacccgct tgatggcgaa gccatggtta
     8041 ttcttgctga agcctatct
//
LOCUS       DAOSFC010000024         7938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7853_length_7938_cov_4.917798, whole genome shotgun sequence.
ACCESSION   DAOSFC010000024 DAOSFC010000000
VERSION     DAOSFC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7853_length_7938_cov_4.917798"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PK791_01275"
     CDS             complement(<1..190)
                     /locus_tag="PK791_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91977.1"
                     /translation="MTYAKGISLIHFIVPPTYEARFNRQRLEDIAKTTISKAIDETNC
                     SFSLRLTNNQGIRSLNRDY"
     gene            complement(168..2408)
                     /locus_tag="PK791_01280"
     CDS             complement(168..2408)
                     /locus_tag="PK791_01280"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOH91978.1"
                     /translation="MLKLKETKKTPSKRTRNLTLAVILVVSSFIAYLFMILPGYLDNR
                     GVQINIGEAATEDILAPYSINFESKVLTERARQNAANAVEDVYLTSDPSIARHQLNVL
                     DAALSYITTVKNDPLSTHEQKIEDLLMMQDLKLSVDDYSKILLMEMNDWQAVGVECSR
                     VLETVLRENLRENQIERARTNLQNIIKFNFKLEQTQITTAIVSDLIVPTSPFSPEETQ
                     RARALAMANVQPITRQIIAGEAIVRRGEILAEADMEALDIFGLTNPTDQTNAFIKNGV
                     AVLISALLIFIFFRYRSTQIMLQPLCMIMVACFFLIFLGLARFLVMGNAVWPYLFPLA
                     AFGLTIGIIFNFELAAILSLILAFLSVLGFGREAELIIFYLVPGLVAMFILGDARRLS
                     TFFGTGFVTGVIAALIVAMFRLGDSYTDWLGLGTLMAAALFNGIATATLALLLQHIFA
                     SILDLPTSLQLMDISRPDHPLLQLLLNNAPGTYQHSLQVSNLAEQAADAIGANRLLVR
                     AGTLFHDIGKSSNASFFIENQIRGNIDSHDESDPYETAQTIIAHVTDGVALAKRYRLP
                     SRIIDFILEHHGNNITRYQYARAVELADGDESLVDEDKFRYPGPTPQSRETAILMLAD
                     GTEARARATNPRTDDEIRSVIDAVVQSVMSAGQLDDTELTLNDMKAIKKSFFETIKRS
                     YHPRVKYPDQRKDDKKEENFDDTKEDSDTVEDKASLEQTEKENSPVKESIFPQETTEI
                     DLREGY"
     gene            complement(2490..3080)
                     /locus_tag="PK791_01285"
     CDS             complement(2490..3080)
                     /locus_tag="PK791_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOH91979.1"
                     /translation="MKTWQHIALGILIGLILAGGILLFILPNRGSPITLVTKTPDLTP
                     QASATLALIRVHVTGAVNEPGLITLPKGACLADAIEKAGGLIEGYDQNLLNLSEPIAD
                     GQRIHIPRLDEQSISNFNQGRSITFSTEESLININTADFETLCLLPGIGPTKAQAIIQ
                     YREENGLFLDIADIQKVKGIGPTLFNTIKDQITIGD"
     gene            complement(3077..4018)
                     /locus_tag="PK791_01290"
     CDS             complement(3077..4018)
                     /locus_tag="PK791_01290"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOH91980.1"
                     /translation="MSTKQKSELKMPAYGGQAVIEGVMMRGKRFLAMAVRNPHGQIQL
                     HEEELPAIYSSKWMRVPFLRGILALWDSLSLGTRMISLSANISGTDDEQIDSKSMALV
                     TVLSLVIGVAVFFLLPAFVAGWLDKRFTLGPWWSNVIEGVLRLVLLIAYMALVGQMKD
                     IRRVYAYHGAEHKTINAYEANVALTPSETAKQSTQHPRCGTSFMLTLALISVLVFSLL
                     GPMPIYWRLISRILMLPLVAGIAYEYIRWVAKYMDDSAFLRWIIKPNLALQKLTTREP
                     SEDMLEVAIAAFNRLLELEEGKDTVKTPEKPDTDLSA"
     gene            complement(4015..5385)
                     /locus_tag="PK791_01295"
     CDS             complement(4015..5385)
                     /locus_tag="PK791_01295"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOH91981.1"
                     /translation="MDKELSAINYFLENQDALMEQYLNVLRIPSVSTDPAHKKDMERT
                     AEFLKDYFEKLGMDYIEIFPTKVHPIVFAEKKSALPDAKTLLIYGHYDVQPPDPLHEW
                     NTPPFEPNKVGDYIYARGASDMKGQIMASIFALESVLKTNDLPINVKFIIEGEEEIGS
                     PSLVDFLEDHTELLKCDMILNPDAGMISATQPTIVYGLRGMVYIELRIDGPKMDLHSG
                     LFGGNIANPANVIADIISKLHNPDGSVAIPGFYDKVRPLTPEERELIAMAGSLEDSYI
                     KLTGVPRLYGEEGFTLLERQGARPTLDVNGLYSGYIEEGAKTIIPAYAKAKISCRLVP
                     DQNPDDIYRLIKSYIASIVPNTVRAQIFKHSLGPAYLADDAPGYENLANALSETWGKP
                     VTLKREGGSIPVATNMQEILGVKSLLTGFGLPDDAIHSPNERQHLPTWTKGVQALIRF
                     LMSFAK"
     gene            5534..6478
                     /gene="trxB"
                     /locus_tag="PK791_01300"
     CDS             5534..6478
                     /gene="trxB"
                     /locus_tag="PK791_01300"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOH91982.1"
                     /translation="MTNENHRQLIIVGSGPAGFTAAIYAARANLAPMLISGMKLYGQP
                     SKTATIENYPGFPNGVEGPELGRLFEEQARAFGAEVVYDAVEKIDLSQKPYQAHTYGG
                     VFTADALILAMGTEYNKLGVPGEKELEGRGISNCATCDGYFTKGKNVHVVGGGDAAIE
                     EAIFLTRFADSVTVIHRRDALRASAILEERARTNPKIKFIWNSVVEEIKGENAVEALV
                     LKNVKTDELSTVPSQGLFVYIGQKPTTEIVGKQLETDEKGYIIIDENMETSVPQVYAA
                     GDVADPHYRQIVTAAGMGAAAAISATRFLETLMVNSAK"
     gene            complement(6498..7646)
                     /locus_tag="PK791_01305"
     CDS             complement(6498..7646)
                     /locus_tag="PK791_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH91983.1"
                     /translation="MKKTSFDEHSHRKNTVCSVCGTRLTDNATRCLVCGTPVSHTGTS
                     SKSRGLDSGAPEITLRLPGMIILALVFLALVAVIVGLLVSSKMSASAKDAEIAESSQT
                     PTATVTLTATPTLTSTPEPTWTPLPPVAHTVAQGQQCLTIALLYNTSVQAIILENKLD
                     ANCTVSEGQVLMVPLPTYTPSPVPSATPEEIVDEERCTNVMPITVTASDTLSSIAANY
                     SVTMKDIMEHNGMSNDIVRAGQALEIPLCYRLPTPGPTPTATPVPPYPGPNLLLPRSG
                     AYFPPSEETVTLQWASVADLYPNELYRVVVKDLTSTDERSLVEYVSDTKFILPASFRP
                     DDDIPHIIQWTVSIARVINNDPANPIYEEAGAQSVPRVFIWSSPNVQQ"
BASE COUNT         1970 a         1714 c         2133 g         2121 t
ORIGIN      
        1 gatagtcacg gtttagactg cggatgccct gattgttcgt caggcgcaag gaaaaactgc
       61 aatttgtctc atcaatggct tttgaaatgg ttgttttggc gatatcttct aatcgctggc
      121 gattaaacct ggcttcataa gtgggcggaa caataaaatg aatcaaacta atacccttcg
      181 cgtaagtcaa tttctgtcgt ttcctgcggg aaaatgcttt ctttcacagg gctgttttct
      241 ttttctgttt gctctaagct ggctttatct tccacagtat ctgagtcttc ttttgtatca
      301 tcaaaatttt cttctttttt atcatctttg cgttggtctg ggtatttaac gcgcgggtgg
      361 taggagcgtt tgatggtttc gaagaaggac tttttaatcg ctttcatatc atttagggtg
      421 agttcggtgt cgtcaagttg gccggctgac atcacacttt gcacgaccgc gtcaatgaca
      481 gaccttatct catcatcagt acgggggttt gtagcccggg cgcgggcttc ggttccgtca
      541 gcaagcatta agatggcagt ttcgcgcgat tgtggtgtag ggccaggata acggaatttg
      601 tcttcatcca ccaaactttc gtcaccatca gcgagttcaa cggctcttgc gtattgataa
      661 cgggtgatgt tgttgccgtg atgttcaagg ataaagtcga taatgcgact gggtaaacga
      721 taacgtttcg ccagtgcaac gccatcggtg acgtgcgcga tgatggtttg tgctgtttcg
      781 taggggtcac tctcgtcgtg ggagtcaata ttgccccgta tttgattttc gatgaaaaag
      841 ctggcgtttg atgatttacc gatgtcatgg aaaagtgttc cggcacgcac aagcagtctg
      901 ttagcgccta tagcgtcagc ggcttgttcg gccaggttgg agacttgcag actgtgttgg
      961 taggtacctg gcgcgttgtt gagcaataac tgcaataagg ggtgatccgg gcgggaaata
     1021 tccatgagtt gcagggatgt gggtaaatcc agaattgagg cgaaaatgtg ttgcaggagc
     1081 agggcaagtg tggcggttgc gataccgttg aacaaggcag cggccattaa cgtgcccaga
     1141 ccgagccagt ctgtgtatga atcgcccaaa cggaacatag cgacaattaa tgcggcaatg
     1201 acaccagtaa caaagcctgt accgaagaag gtggacaatc tgcgcgcatc tcccagtata
     1261 aacatggcaa ccagtcctgg aacgagataa aagattatga gttcggcttc gcggccaaag
     1321 ccaagtacag ataaaaaggc aagaatcagg cttaggatgg cggccagttc aaagttgaaa
     1381 atgataccga tggtcaagcc aaatgcggcg agcgggaata agtaaggcca tacggcgttg
     1441 cccatcacca aaaaccgggc aagacctaaa aagattagga agaaacaggc caccatgatc
     1501 atgcataaag gttggagcat gatttgggtg ctgcgatacc taaagaagat gaaaatcaac
     1561 aaggcggaaa tcaaaacagc aacaccgttt ttgatgaaag cattggtttg gtcggtgggg
     1621 ttggttagtc caaagatgtc cagggcttcc atatcggctt cagcgagaat ttcgccacgt
     1681 ctgacgatgg cttcgcccgc aataatctga cgggtgatgg gctgcacgtt tgccatggcc
     1741 agcgcgcgcg cgcgttgggt ttcttcaggg ctgaaggggc tggttggaac gattaagtcc
     1801 gacacaatgg cggttgtgat ttgggtttgc tccagtttga aattaaattt gataatgttt
     1861 tgcaggtttg ttcgggctct ctcaatttgg ttttcgcgca agttttcacg caatacggtt
     1921 tcaagcacac gcgaacattc cacgcctacg gcttgccagt cgttcatttc catcaagaga
     1981 attttggaat aatcgtccac gcttaatttc aagtcttgca tcatcagcaa gtcttcaatt
     2041 ttttgttcat gtgttgagag tgggtcgttt ttaacggttg tgatatagct tagggcagca
     2101 tccaagacat tgagttggtg gcgggcgatg cttgggtcag atgtgagata gacatcttca
     2161 acggcgttgg ctgcattttg cctggcgcgt tcggtaagca cttttgattc aaagttgatg
     2221 gaataaggcg caaggatatc ttcagtggct gcttcgccga tgttaatctg cacgccacgg
     2281 ttatcgagat aaccgggcag tatcataaac aaatacgcga taaaactgct caccactaaa
     2341 ataacagcga gcgtgagatt gcgtgttcgt ttggatggtg ttttttttgt ttcttttagc
     2401 tttaacataa tgtcttagcc ttgggttaat ttgtttttgc aaaaaccacc ctaattatac
     2461 ccgcaagatg gcatgtctgc actttttgtt caatcaccta ttgtgatttg gtctttgatg
     2521 gtgttaaaca gggtaggacc gatccctttc actttttgaa tatcagcgat atccaggaat
     2581 aatccgtttt cttcgcgata ttgaatgatt gcttgtgcct tggtggggcc gatgccaggg
     2641 agaagacata aggtttcaaa gtcagcggta ttgatattga tgagactttc ttcggtgctg
     2701 aaggtgattg accgtccttg gttaaagttg ctgatacttt gttcgtcaag gcgtgggatg
     2761 tggatacgtt gcccgtcagc gataggttct gagagattga gtaagttttg gtcgtagcct
     2821 tcaatcaatc cacctgcttt ttcgatggca tctgccaggc aggcgccttt gggcaatgtg
     2881 attaagcctg gctcattcac cgcgcctgta acgtgcacac ggatcagcgc cagtgtcgca
     2941 ctggcttgtg gggtcaggtc gggagttttg gtgactaatg tgattggcga accacggtta
     3001 ggcaggataa acagcaaaat gcccccggct aaaatcaagc cgatgagaat gccaagggca
     3061 atatgctgcc aggttttcat gccgataaat ccgtgtcagg cttttcaggc gttttgacgg
     3121 tgtctttgcc ttcttcaagt tccagcaaac gattaaaggc cgcgatggca acttcaagca
     3181 tatcttcgga aggttcgcgc gtggttagtt tttgaagagc caaatttggt ttgatgatcc
     3241 agcgcagaaa agctgaatcg tccatatatt ttgccaccca gcgaatgtat tcataggcaa
     3301 taccggcaac aagtggcaac atgagaatac gcgaaattaa tcgccagtag atgggcatgg
     3361 gccccaacaa ggagaagacc aaaacactaa taagcgccag agtcagcata aatgaggtgc
     3421 cacaacgtgg gtgttgggtg ctttgtttgg ccgtttcgga tggggtgagc gcgacgttgg
     3481 cttcataggc gttaattgtt ttgtgttccg cgccatgata ggcgtataca cggcgaatgt
     3541 ccttcatctg accgactaaa gccatatagg cgattaacag gacaagacgt aaaacgcctt
     3601 caataacgtt actccaccaa ggccctaagg taaagcgttt gtccagccag ccagcaacga
     3661 atgccggcaa gagaaagaaa actgccacac cgatgaccag ggacaggacg gttaccagtg
     3721 ccatgctttt ggagtcgatt tgttcatcat ctgtgccaga aatgttggct gacaggctaa
     3781 tcatgcgcgt gccaaggctt agggaatccc acaaagccaa aataccgcgt aaaaacggga
     3841 cgcgcatcca tttggaactg taaatcgcgg gcaattcttc ttcgtgaagc tgaatttgac
     3901 cgtgcgggtt gcgaacagcc attgccaaaa agcgtttgcc acgcatcatg acgccttcaa
     3961 taacagcttg cccgccatag gcgggcattt ttagttcgga cttttgttta gtgctcactt
     4021 agcaaaactc atcaaaaatc gaatcaaggc ttgcacaccc ttggtccagg tgggcaagtg
     4081 ttggcgttcg ttgggcgagt ggatagcgtc atcgggcaat ccaaagccgg tcagaagcga
     4141 ttttacgccc agaatttcct gcatattagt ggcaacagga attgaaccgc cttcgcgttt
     4201 caaagtaacg ggtttgcccc aggtttcgct tagcgcattt gccaggtttt catagccggg
     4261 cgcgtcgtct gccagatagg cgggacccaa ggagtgtttg aagatttgag cgcgcaccgt
     4321 gttgggtacg atgctggcaa tgtagctttt aataagccgg tagatatcat cagggttttg
     4381 gtccggtact aaacgacatg aaattttcgc tttggcataa gcaggaataa tggtctttgc
     4441 gccttcttcg atgtaaccag agtaaagccc atttacgtcc agggtaggcc tggcaccctg
     4501 gcgttcgagt aaggtgaagc cttcttcacc ataaagtctg ggaacgccgg tgagtttgat
     4561 gtaagaatct tcaagcgaac cagccatggc gatgagttca cgttcttctg gggtaagcgg
     4621 gcgcacttta tcataaaaac cgggaatggc gaccgagcca tccgggttgt gcaatttcga
     4681 gatgatgtcg gcaatgacgt tggcggggtt ggcgatgttg cctccaaaca agccactgtg
     4741 caagtccatc ttgggtccat caatgcgcag ttcaatgtaa accataccac gcaaaccata
     4801 tacaatggtg ggctgggtgg cggaaatcat gccagcgtct gggttgagaa tcatgtcgca
     4861 cttgagaagt tcggtatggt cttccagaaa gtctacaagt gatggtgagc cgatttcttc
     4921 ttcgccttca atgatgaatt taacgttgat gggcaggtcg ttggttttca agacgctttc
     4981 cagggcgaaa atggaagcca tgatttggcc tttcatatcc gacgcgcccc gtgcgtagat
     5041 gtaatcaccg actttgttgg gttcaaaggg tggggtgttc cactcgtgga gtgggtcagg
     5101 tggttgtacg tcgtaatgcc cgtagatgag caaggttttt gcatcgggca gggctgattt
     5161 tttctccgcg aaaacaatgg ggtgaacttt tgtggggaag atttcaatat agtccatgcc
     5221 cagtttttcg aaatagtcct tcaagaattc agccgtgcgt tccatgtctt ttttatgggc
     5281 tggatcagtt gaaacggagg gaattctaag gacattaagg tattgttcca tcaaggcatc
     5341 ttgattttct aaaaaatagt ttatggcaga tagttcttta tccataatta cctcttcttt
     5401 ttgtttgctt tatcgctata cattatatgc caaactgcga attatcgtga taggattagc
     5461 atgaaaaaag atcgaccctt caaattgaac tatagggtca ttataagtaa caataaaaaa
     5521 tgaaggaaat attatgacaa acgaaaatca tcgacaactc atcatcgtcg gttctggacc
     5581 agccggtttt actgccgcaa tttacgccgc gcgggcaaat ttagccccca tgctcatctc
     5641 ggggatgaag ctttacggac agccttccaa aaccgctacc atagaaaact atccgggttt
     5701 tccgaacggt gttgaagggc cagaactcgg ccggcttttt gaagaacaag cacgcgcctt
     5761 tggtgctgaa gtggtttatg acgcggttga aaaaattgat ttgagccaaa aaccctatca
     5821 ggcgcatacc tatggaggcg tttttaccgc agacgctctt attttggcaa tgggtacaga
     5881 atacaataaa ctaggcgttc ccggtgaaaa agaactggaa ggacgcggta tttcaaactg
     5941 cgcgacttgt gatggctatt tcacaaaagg taaaaatgtg catgtggttg gtggcggaga
     6001 cgccgcgatt gaagaagcca tcttcctgac tcgctttgcc gacagtgtta ccgttatcca
     6061 ccgtcgagat gctttacgcg ccagcgctat ccttgaagaa cgcgcgcgta caaacccgaa
     6121 aatcaagttc atctggaatt ccgtcgtgga agaaatcaaa ggcgaaaatg ctgttgaagc
     6181 gcttgtctta aaaaatgtta aaactgatga attgagcacc gtacccagcc agggtttgtt
     6241 tgtttacatc ggtcaaaagc ccaccacaga aattgtgggt aaacaattag agacagacga
     6301 aaagggctat atcattatcg acgaaaatat ggaaaccagc gtgccccagg tttacgcggc
     6361 aggtgatgtt gccgacccgc actaccgcca aatcgtaacc gcggcaggca tgggagccgc
     6421 cgcggcgatt tcagcaacgc gttttttgga aacgctgatg gttaattcag ctaagtagcg
     6481 cggtagtccc gtttggctta ttgctgaacg tttggactac tccaaataaa tactcttggt
     6541 acactttgcg caccggcctc ttcgtaaatc ggattggccg gatcgttatt aatgacccgg
     6601 gcaatggaca ccgtccactg aatgatatgt gggatgtcat catcaggcct aaagctggca
     6661 ggcagaatga atttcgtgtc gctaacatat tccaccagac tgcgttcgtc ggttgatgtc
     6721 aggtccttga ccacaacacg gtaaagttcg tttggataaa ggtcagccac cgaagcccat
     6781 tgcagggtaa cggtttcttc gctgggtggg aagtaagcgc ccgagcgggg caagagcaag
     6841 tttggtccgg gataaggcgg caccggtgtc gcggtggggg tgggtccagg ggtaggcagg
     6901 cgatagcaca ggggaatttc cagcgcctga cccgcgcgta caatgtcgtt gctcatgccg
     6961 ttgtgttcca tgatgtcttt catggtcacg gagtaattag cggcgattga gctcagtgtg
     7021 tcggaagcgg taacggtgat gggcatcacg ttagtacagc gttcttcgtc aacaatttct
     7081 tccggtgtgg cggatggtac cggcgatggg gtgtaggtgg gcaggggcac cattaagacc
     7141 tggccttctg aaaccgtaca gttggcatca agtttgtttt caagaatgat ggcttgcacc
     7201 gatgtgttat agagcaaagc aatggttaag cactgctggc cctgtgccac ggtgtgggcg
     7261 acagggggca ggggcgtcca ggtgggttcg ggcgtgctgg taagcgttgg cgtggcggtc
     7321 agcgttacgg tggcggtggg cgtttgagag ctttcagcga tttccgcatc tttggcggag
     7381 gcagacattt ttgagctaac cagcagacca acgatgacag ctaccagcgc cagaaagaca
     7441 agcgctaaaa taatcatgcc gggtaatctg agcgttatct caggcgcgcc actgtccagc
     7501 ccgcgtgatt ttgagcttgt gcccgtgtga ctcactggcg taccacagac caaacagcgt
     7561 gtcgcgttat cggtcaggcg ggtgccacat actgagcaga ccgtattctt tctatgtgag
     7621 tgttcatcaa aacttgtttt cttcattgtt ttatccaatt cgttttcttt catatcaggg
     7681 aatgcacagg taaaataggc atatgactgc ggtttatatt cacattccct tttgtaaacg
     7741 tcgttgtaac tattgtgatt ttatcacgta tgccaatatt gaggctatgc taccagatta
     7801 tctaagcgca ttgcaaatgg aaattttaaa ttccaaatgg gagtgtgacc aggctgaaac
     7861 ggtttacatc ggtgggggaa cgccatcttt gatgagtccc gagcaagtcg ccggcctttt
     7921 ggaagccgtg cgtcaacg
//
LOCUS       DAOSFC010000025         7915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_7889_length_7915_cov_4.800127, whole genome shotgun sequence.
ACCESSION   DAOSFC010000025 DAOSFC010000000
VERSION     DAOSFC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7889_length_7915_cov_4.800127"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PK791_01310"
     CDS             complement(<1..218)
                     /locus_tag="PK791_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91984.1"
                     /translation="MNIILDAHQDLAYNMLALGRDYRRSAYETRQTEVGKPFMAWTDD
                     ALIGWPEYMQARVAIVFSTLFAAPARSVP"
     gene            complement(284..1738)
                     /locus_tag="PK791_01315"
     CDS             complement(284..1738)
                     /locus_tag="PK791_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOH91985.1"
                     /translation="MSENDAVSVQGSQDTEVPEVSPADQAIEPQSEAIQEPAEEKKVL
                     SMEDLMDQLDDDAFIIDLPKAGEIRDGIIASISEGQILVSVGAKSEGIITGKEYEAIP
                     QDFLNTMEVGKAIPVYVITPEDSSGNLILSFIRAVEEQSWVDAEALLKSGEAFDSKIV
                     GYNKGGLIVMLSTIRGFIPASQLSFSRRLGISGDTPEAKYKDLIGEDITVCVIEVDRD
                     RHRLILSERAASSETRDQIRDMVIESLEEGEIRKGRITSIADFGAFVNIGGADGLVHM
                     SEITYDHITHPSQKLEVGKEVKVKVISVDRDRKRIGLSIRALKKDPWLDYVKDLTVGQ
                     LVEAEITRLTSFGAFAKLNVPGDLEGLIHISELSDRRVDHPKAIVKSGDQVTLRIIKI
                     DEEEHRIGLSLRRVDSVAYADFDWKTLAADFDLLGDEEGEALADIVGEENVAEAEELA
                     EAVEEKAETVEETVEEPAEEAAEEVKEDEETPEA"
     gene            complement(1878..2990)
                     /locus_tag="PK791_01320"
     CDS             complement(1878..2990)
                     /locus_tag="PK791_01320"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOH91986.1"
                     /translation="MNAKRIPVSDPHASYLSYKNEIDAAIHKVLDSGWYILGEVTREF
                     ESNFADYIGVKHCVGVNSGTDAISLALRALDIGAGDEVITVSLTAVATVAAIDLVGAT
                     PVLCDIDPDTRYMDASKVQGLISEKTKAILPVHLYGHPADLPALQAIAKKHNLFLIED
                     CAQAHGAQIDGKMIGSFGDLACFSFYPTKNLGAFGDGGAVCTNNDALADRLRWLREYG
                     WKERYISYYKGMNTRLDEMQAAILNVKLKHLEHDTQKRIAIAQTYQHALAGLDLKLPS
                     TKPGYRHVYHLYVVEANHREGLQTYLRDNGIATAIHYPQAVHQQPAYSQKIKGFHDLP
                     VTNSLVPKILSLPMYPELSEDKQEHVIEMIRRFYSN"
     gene            complement(2992..3978)
                     /locus_tag="PK791_01325"
     CDS             complement(2992..3978)
                     /locus_tag="PK791_01325"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH91987.1"
                     /translation="MDLSTFKNKNVLITGGLGFIGSNLAIRLVEAGANVTLVDSLIPT
                     YGGNLFNIDPIKHLAKVNISDVRDPHSMKYLVAGQDYLFNLAGQTSHLDSMTAPENDL
                     NINVLAQLHILEACRHHNPDLRLVFASTRQIYGKPLQIPVDETHPLNPVDVNGINKLA
                     GEQYHRLYYDVYGIRSTILRLTNTYGPRMRIKDARQTFLGIWIRRLLEGLPLQIYGDG
                     KQVRDYNYVDDVVDALLITALSDNSYGEVYNLGSTEDLSHTETAQIMTQLHEGSFEYV
                     PFPPARKSIDIGDYQGSFEKIKNQLGWEPRTSFRDGMGKTIAYYQEHFENYL"
     gene            complement(3993..4748)
                     /locus_tag="PK791_01330"
     CDS             complement(3993..4748)
                     /locus_tag="PK791_01330"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH91988.1"
                     /translation="MTTNAEQTQKISSITAVFPAYNDAGTIPSMVLTALIALRQVTDD
                     YEVVVTNDGSKDATGVVLQELASRFPELKVITHETNLGYGCALRSGFAAATKDWIFYT
                     DGDAQYDPLEMPKLAKALKDGIDVVNGYKISRNDAWYRILIGRIYHHVVKIAFGFKLR
                     DVDCDFRLIRREIFDNIELESKTGTICLEMVKKFQDAGYKFAEVPVNHYHRSYGKSQF
                     FRPKHLWTTFKHLSQLWWKLVVKKEHLANKSQS"
     gene            complement(4748..5233)
                     /locus_tag="PK791_01335"
     CDS             complement(4748..5233)
                     /locus_tag="PK791_01335"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOH91989.1"
                     /translation="MKNFEEGFANGQKQEKSHSKNGFIALIQKLWNARFVRFLFVGAL
                     NTLFSYLLYAFLVFIGINYVWARVISIILGIIFNFFTTGRIVFNNKDNWLIIRFILVY
                     AVTMSLDVFVLKRLVENMTINKYLAGALTTIPIALLSFLLNSVFTFKTIKLCKKPPKD
                     T"
     gene            complement(5272..6741)
                     /locus_tag="PK791_01340"
     CDS             complement(5272..6741)
                     /locus_tag="PK791_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOH91990.1"
                     /translation="MPEPEDILNPPARTPVDQENDEDRARVIEETLRSFNAPGTVVEI
                     RRGPAITMFGVEPDYLETRSSRTRVRVSNITPLADDLAMALKASRIRIQAPVPGKGYI
                     GIEVPNTQTSIVSMLEIFQSTAFQSDKSALRFVLGKDVTGLPFTSDLAKMPHLLIAGT
                     TGSGKSVCVNAIIAGYLLTLTPDQLRMMLVDPKRVELTAYNGIPHLLTPVITDADKVV
                     TALQRMLLEMDARYQLFAGKGVRNIEEYNQQAQAQGDKELPYILVVIDELADLIMLSP
                     NETQTSLARLGQLARATGIHLIISTQRPSVDVLTGLIKANFPARIAFAVASGSDSRVI
                     LDQMGAERLLGRGDMLFLPPDAPEPIRLQGTYVSDEEINRLTDFWRNQSAQSDAPERP
                     DLMTAASTPHVPLVDDAKWHIEEPDPDADPIEDEVIVMIRKEGRASISMLQRKFRIGY
                     TRSARIIEKLEADGIIGPPSPSGTREVLDYGQYPPVNED"
     gene            complement(6996..7385)
                     /locus_tag="PK791_01345"
                     /pseudo
     CDS             complement(6996..7385)
                     /locus_tag="PK791_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
     gene            complement(7837..7909)
                     /locus_tag="PK791_01350"
     tRNA            complement(7837..7909)
                     /locus_tag="PK791_01350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7874..7876),aa:Arg,seq:tcg)
BASE COUNT         1978 a         1691 c         2086 g         2160 t
ORIGIN      
        1 ggcacactgc gggcaggcgc agcgaaaagt gttgaaaaaa cgatagcgac tcgggcttgc
       61 atgtattctg gccagccgat tagagcatcg tcggtccatg ccataaaagg cttccccact
      121 tcggtttggc gggtttcata ggcagagcgg cgataatcgc ggccgagagc aagcatgttg
      181 taggctaagt cttggtgggc gtcgaggata atattcatgg tttgattata cacaaaaaag
      241 cgccagctat tgtgccggcg ctttaatatt gctttttgaa tctttaggct tcaggcgttt
      301 cttcgtcctc tttgacttct tcagcagctt cttcggcggg ttcttctaca gtttcttcaa
      361 cagtttcagc tttttcttca actgcttccg ctaattcttc tgcttcagcc acattttcct
      421 cgccaacgat atcagccagt gcttcacctt cttcatcgcc aagaagatcg aaatcagcag
      481 cgagagtctt ccagtcgaag tcagcgtagg ctacagagtc gacacggcgc aggctcaagc
      541 caatgcgatg ttcttcttcg tcaatcttga taatgcgcaa agtaacctgg tcgccagatt
      601 tgacgattgc tttggggtgg tccacgcggc ggtctgaaag ttcagagata tggataagcc
      661 cttcaaggtc accaggaacg ttgagtttgg caaatgcgcc aaagctggtc aggcgggtga
      721 tttcagcttc aacaagctgg ccaacggtca ggtctttgac ataatcaagc catgggtctt
      781 tcttaagcgc gcggattgag agtccgatgc gtttgcggtc gcggtcaaca ctgataacct
      841 tgaccttaac ttctttgccc acttcaagtt tttggctggg atgggtaatg tggtcgtagg
      901 tgatttcgga catatggaca agcccatcag cgccaccgat gttcacaaac gcaccaaaat
      961 cagcgatgct ggtgatacga cctttgcgga tttcgccttc ttccaggctt tcgataacca
     1021 tatcgcgaat ttggtcgcgg gtttcggaac tggcagctcg ttcgctgagg attaagcgat
     1081 ggcgatcgcg gtcaacttcg ataacgcaaa cggtgatgtc ctcgccaatc aagtctttat
     1141 atttcgcttc gggagtatca ccggaaattc ccagtcgtcg gctaaagctt agctgagatg
     1201 cgggaataaa accgcgaatg gtagaaagca ttacgataag cccacccttg ttatagccta
     1261 cgattttgga atcgaaagct tcacctgatt tcaacagggc ttcagcgtcc acccagcttt
     1321 gttcttcaac tgcgcggatg aatgaaagga tgagattgcc acttgaatct tcaggtgtaa
     1381 taacatagac aggaatggct ttgcccactt ccatcgtgtt caggaaatcc tggggaatgg
     1441 cttcgtattc tttaccagtg ataatacctt ctgatttggc tccgacactg actaaaatct
     1501 ggccctcaga gatgctggcg ataataccgt cgcggatttc gcccgctttg ggtaaatcaa
     1561 taataaaagc atcatcatca agctgatcca tgagatcttc catggataag acttttttct
     1621 cttcagcagg ttcttggatt gcttcggatt ggggttcgat tgcctggtcg gctggactaa
     1681 cttcagggac ctcagtatct tgagaccctt gcacactaac tgcgtcgttt tctgacatta
     1741 aaaatagctc caattgatta atttggctta tattatagat gcataaacca aatctcgcaa
     1801 gtgaaactga atatattgtt gtaaacagtc tgtaaactta ctctttactg attaggaata
     1861 taaacaaaag caaacaatta gtttgaataa aagcgtctaa tcatttcaat gacatgctct
     1921 tgcttgtctt cactcaattc agggtacatg ggcaagctga gaattttggg aacaagagag
     1981 ttagtgacgg gcaaatcgtg aaagcccttt attttttggc tatatgcagg ttgttggtga
     2041 acggcctggg ggtaatgaat agccgtggca atgccattgt cgcggagata agtttggagt
     2101 ccttcgcgat ggttggcttc aacaacatat aggtgataaa catggcgata tccaggtttg
     2161 gtgctgggca gttttaggtc taaaccggca agggcatgct gataagtttg cgcgatggcg
     2221 attcgttttt gggtgtcgtg ttcaaggtgc ttgagtttaa cgttcagaat ggcggcctgc
     2281 atttcgtcta agcgggtatt catgcctttg taatagctaa tgtagcgttc tttccagcca
     2341 tattcgcgca accagcgcaa gcggtcagcc agggcatcgt tatttgtgca caccgcgcca
     2401 ccgtcaccaa aagcgcctaa atttttggtg ggataaaagc tgaagcaggc caaatcgccg
     2461 aaactgccaa tcattttgcc atcaatttgg gcgccgtggg cctgggcgca atcttcgatc
     2521 aggaaaagat tgtgtttctt ggctatggct tgcaaggcgg gcaaatcggc gggatgtccg
     2581 taaaggtgga cgggcagaat ggcctttgtt ttctcactta ttaagccttg aactttgctg
     2641 gcgtccatgt agcgtgtgtc cgggtcaata tcgcacagga cgggtgtcgc gccaacgaga
     2701 tcaatggcag cgacggttgc cacggcggtt agcgagacgg tgatgacttc atcacctgcg
     2761 ccgatatcca gcgcgcgcaa agccagcgaa atggcatctg tgccagaatt aacgccgacg
     2821 caatgtttta cgccgatgta atctgcgaaa ttactttcaa attcgcgggt cacttcgccc
     2881 aggatgtacc agccgctgtc aagtactttg tggatggcgg cgtcaatttc gtttttgtaa
     2941 ctaagataac tggcgtgtgg gtctgaaact ggaatacgtt ttgcgttcat attaaagata
     3001 attctcaaaa tgttcctgat aataagcgat ggttttaccc attccgtcgc ggaagctggt
     3061 gcggggttcc cagccgagct ggtttttgat tttttcaaaa ctgccttgat agtcgccgat
     3121 gtcaatagat ttacgtgcgg gcgggaaagg cacatactca aaactgccct catgaagctg
     3181 ggtcatgatt tgggcggttt cagtatgaga aaggtcttca gttgagccga gattgtaaac
     3241 ttcgccatag gaattatccg acaaggcggt gatgagcaac gcgtctacaa catcatcgac
     3301 gtagttgtag tcgcgcactt gtttgccatc gccatagatt tgcagtggca agccttcaag
     3361 caggcgtctg atccaaatgc ccaggaaggt ttgacgggcg tctttaatgc gcatacgcgg
     3421 tccgtaggtg ttggtaagac gcaaaatagt tgaacggatg ccgtagacat catagtaaag
     3481 gcggtgatat tgttcgccgg ccagtttgtt gatgccgttg acgtctacag ggttgagcgg
     3541 gtgggtttca tccacgggta tttgcagtgg tttgccataa atttggcgcg tgctggcgaa
     3601 caccaggcgc aaatccgggt tgtgatgtcg gcaggcttcc aaaatgtgca gttgggcgag
     3661 tacgttgatg tttaaatcat tctccggggc ggtcatagaa tcaagatgac tggtttgacc
     3721 tgccaggttg aagagataat cttgccccgc gaccaggtat ttcatggagt gggggtcacg
     3781 cacgtctgag atgttcacct tggcgagatg ttttatggga tcaatgttga acagatttcc
     3841 gccataagtc gggatgagac tgtccaccag ggtgacattc gcgccggctt caaccaggcg
     3901 aatggccagg tttgaaccga taaagcccag tccaccggtg atgagcacat ttttattttt
     3961 gaaagttgaa aggtccatag gctaattgta ctttagcttt ggcttttatt cgcaaggtgt
     4021 tcttttttga cgacaagttt ccaccaaagt tgcgaaagat gtttgaaagt tgtccacaag
     4081 tgctttggcc tgaagaactg cgatttgcca tatgaacggt gatagtgatt tacaggtact
     4141 tcggcaaatt tgtagccggc atcctggaac tttttgacca tttccaggca aatggtaccg
     4201 gttttgcttt caagttcaat gttgtcgaat atttcacggc gaattagccg aaaatcgcag
     4261 tcaacatctc ttagtttaaa tccaaaggcg attttaacaa cgtgatggta aattctgcca
     4321 attaaaatgc gataccaggc atcgttgcgg ctgattttat aaccattgac gacatcaata
     4381 ccgtctttta gcgctttggc tagcttaggc atttcgagtg ggtcatattg cgcatcgccg
     4441 tcagtataaa aaatccagtc ttttgtagct gcagcgaaac cgctgcgtaa ggcgcaacca
     4501 tagcccaggt tggtttcgtg ggtgataact tttaactccg gaaagcgcga agccagttcc
     4561 tgtaaaacca ctccggtagc atctttgctg ccatcgttgg tgacaacaac ttcataatca
     4621 tcggtaacct gacgcagggc gataagcgcg gtgagaacca tgcttggaat cgtgccggcg
     4681 tcgttgtaag ctgggaaaac ggcggttatg ctggatattt tttgcgtttg ttcggcgttt
     4741 gtggtcatca tgtatccttt ggcggttttt tacacagttt aatcgttttg aaagtgaaaa
     4801 cggaattgag caagaaagaa agtagcgcga tgggaatggt ggttaacgcg ccagccaaat
     4861 atttattgat ggtcatgttt tctaccagtc gtttaagcac gaagacatcc agtgacatgg
     4921 ttaccgcata aacgagaata aagcgaatga ttagccagtt gtctttatta ttgaaaacga
     4981 tgcgaccggt ggtgaaaaag ttgaagatga tgccaaggat gatggaaata acccgcgccc
     5041 aaacataatt gatgccaata aagaccaaaa aagcataaag caaatagctg aaaagcgtat
     5101 taagcgcccc aacaaaaaga aagcgaacaa aacgcgcgtt ccataacttt tgtattaagg
     5161 cgataaaacc gtttttggaa tggcttttct cttgtttttg cccgttcgca aaaccctctt
     5221 caaagttttt catctcggct attatacatg aagccgaaag aattgttagg gttaatcttc
     5281 gttgacaggc gggtattgac cgtaatcaag cacttcgcgg gtgccactgg gacttggagg
     5341 gccgatgata ccgtcggctt caagcttctc gataatgcgc gcggaacggg tgtagccaat
     5401 gcggaatttg cgctgaagca tggatattga cgcgcgacct tctttgcgaa tcatgacgat
     5461 aacttcgtct tcgatgggat ccgcgtcggg gtcaggttct tcaatatgcc acttggcgtc
     5521 atcgacaagg ggtacgtgtg gggtagatgc agctgtcatt aggtcagggc gttctggcgc
     5581 gtctgattgt gcgctttgat tgcgccaaaa gtcggtcaga cggttgattt cctcatcgga
     5641 aacataggtg ccctgcaaac ggatcggttc aggcgcgtcg ggtggcagga aaagcatatc
     5701 gccacgacca agtaaacgtt ccgcgcccat ttggtcaaga atgacgcggc tgtccgagcc
     5761 ggaagcgacc gcaaaggcaa tccgggcggg gaagttggct ttgatgaggc cagtaaggac
     5821 gtcaactgaa ggtcgttgag tggagataat gagatgaatg cctgtcgcgc gtgccagttg
     5881 tcccaggcgg gcgagcgagg tttgggtttc gttaggagag agcataatca ggtcggctaa
     5941 ctcatcaatc accacaagga tataggggag ttctttatcc ccttgtgcct gggcttgctg
     6001 attgtattct tcaatattac gtacgccttt gccagcaaag agttgatatc gcgcgtccat
     6061 ctcgagtagc atgcgctgga gagcggtgac gactttgtcg gcatcggtga tgaccggtgt
     6121 taagagatgg ggaatgccat tataagcagt gagttcaacg cgtttggggt caaccagcat
     6181 catacgcagc tggtcggggg tgagtgtaag caaatagccg gcgataatgg cgttcacaca
     6241 tactgattta cctgaacccg tggtaccggc aatgagtaaa tggggcattt tggcaaggtc
     6301 agaggtaaaa ggcaagcccg ttacgtcttt gccaagtacg aaacgcaagg cagatttgtc
     6361 ggattgaaat gctgtgcttt ggaagatttc aagcatcgag acgatggagg tttgggtgtt
     6421 gggaacttca atgccgatat aacctttgcc aggaaccggc gcctggatac gtatccggga
     6481 tgctttcagt gccatagcca ggtcatctgc aaggggagtg atgttgctga cgcgcacgcg
     6541 ggttctgctg ctgcgcgttt caagatagtc cggttcaacg ccgaacatgg tgatggctgg
     6601 tccgcgcctg atttcaacaa ccgtgccagg cgcgttgaaa gaacgcaaag tttcttcgat
     6661 gacgcgagcg cggtcttcgt cattttcctg gtcaacaggc gtgcgagcgg gaggattgag
     6721 aatatcttcc ggttcgggca agacccatga tttttcttct gtgctgcctg ttttgtgaga
     6781 gtcgggttta caaaactgcg cttttctgtg acgggttttg gttcgggatc aagttccaaa
     6841 ggtgcttgta tgggcgtttg gcttgcctgg gaatcgagaa cttgctgaat ctgttggcga
     6901 tgggcttgtt tttgtttgcg atgctctatc agtgattgaa taaacagaga aagtttggtg
     6961 ttggtctttt taagttgtaa aaccgtccag gcaagaaggg cgggaagtga tagattgacc
     7021 aaaagcacca gggcaattaa cagccacgcg atgagcataa tcaccgcgcc aggcgtgcct
     7081 aatccgtgat gaaaccaact gtagatggca ttcccaacga cgccacctcc tttaaagggc
     7141 actgcgcggg tggggtcagg ggcatgaaag aactgaaacc aagtgatgag attgaaaacc
     7201 aaagcgatgg caccgatgag tctgggaatg gtaacaagcg gaaattgttc atagcgcgat
     7261 aagagaaacc aggcaccggt tattatcatg accatgggga tgacgtaaga gaagatgccg
     7321 gtttgtttga gtaaataatc gggccatttg gcgaagaaac tgccttgctt ggcaaaaaag
     7381 gtcatgatca aactgatgat gccgaatacc aaaagaatga taccgctgac ttcaatgact
     7441 ttttcttttg aaaagtcaaa ttgaaagtcc agaaagggtt ttttgttgct ggttcttttt
     7501 ttagagcttg attttgccct ggtggttgat tttgatttcg ctttgcttgt ggctggcttt
     7561 tttgccatga aaactcctgg ttatatctta attagtgttt ctgttataga aaggtcattc
     7621 gtttttctgt acttgtttca gtaaatatca tgcacttttt atgccaagag aaattaataa
     7681 aaattcataa tttgtcttaa tgattgcgtg cttgaaatca ttgtttttaa ctacaaagaa
     7741 acagtcaaag tttcagcgtc agatgctcag tcatgggcat aaccatggag ccttacctat
     7801 atatcgcgct ctctaacgag taaaaaacat aaaaaatacc cgcggaggga ctcgaacccc
     7861 caaccatctg attcgaagtc aggtactcta tccattgagc tacgcgggca agttg
//
LOCUS       DAOSFC010000026         7821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_8045_length_7821_cov_4.875225, whole genome shotgun sequence.
ACCESSION   DAOSFC010000026 DAOSFC010000000
VERSION     DAOSFC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8045_length_7821_cov_4.875225"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /gene="groEL"
                     /locus_tag="PK791_01355"
     CDS             complement(<1..885)
                     /gene="groEL"
                     /locus_tag="PK791_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOH91991.1"
                     /translation="MAKQLVFGEEARRYLKSGVDAVANAVATTLGPKGRNVALDRKYG
                     SPTITHDGVTVAKEIELENPFENMGAQLLKEAATKTNDIAGDGTTTSTVLAHAIVTEG
                     LKNLAAGANPMLLKRGIESATKVVSQAIHKQAIAITTKEEIGAVATVSAQDTQIGDLI
                     ADVMDKVGKDGVITVEESKGLEFETEYVDGMQFDRGYISPYFVTDPEHMLADIEDPYI
                     LIYDKKISAAADIVPLLEKMVQIGKRELLIIAEDIDGEALATLVLNKIRGMLNVVAVK
                     APGFGDRRKAMLQDIAILT"
     gene            complement(916..1206)
                     /gene="groES"
                     /locus_tag="PK791_01360"
     CDS             complement(916..1206)
                     /gene="groES"
                     /locus_tag="PK791_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOH91992.1"
                     /translation="MTIKMRPLGSRLVIEPIEQEDITAGGIVLPETAKEKPQKGTVLA
                     IGPGERNDAGERIPMDVKVGEVVLYAKYSGTEIKYDGKKLLILRESDVLAIL"
     gene            complement(1337..2785)
                     /locus_tag="PK791_01365"
     CDS             complement(1337..2785)
                     /locus_tag="PK791_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH91993.1"
                     /translation="MYINERKNLFHKPKPASNPYLIMFILLCIVIVIAVMRDYTSGKI
                     WPPFVPTPTPTRTVNSFLVEGDTHFQAGNLDAAIDSYNKAILLEPENVDVRVNLARIQ
                     VYSSASLTTDQERRDRLADALAVVDEARELAPQNADALAVRSFVLNWSANPALAGDRT
                     KELRNAAEADALAAIQLDSSNALAKAYYAEVLIDQFKWGQAQDYIAQARENGEHLMDV
                     RRVQAFVYEVLQEYNLAIDWYQKAIDVTPNLTFLYIRVGTLYRHNGGDVNFEIALEYF
                     AKAANLNSQLGIEDPIPYMAIANTYIRMGEAMIASRNAYRALSINPYNPSTYGQVGVI
                     YYKARNYEGAEMVLKCAVTGCNAEESCLAREEDPCQSDITTEKLPLSDSTVVYYYIYG
                     SVLAGLHRSGQDDRCALASEVFKEIRERYYDTSNEFTRTVLGIVSASENICGITPGQV
                     FSTPVPTRDYISTPTPQPTETPLVIPTFTPTP"
     gene            complement(2798..4108)
                     /locus_tag="PK791_01370"
     CDS             complement(2798..4108)
                     /locus_tag="PK791_01370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH91994.1"
                     /translation="MIIDAMYLKGSKFSIQSRRKRGNPWLILFLVLAIAGLVYLNVVV
                     VPMINPPFVPTPTPTRDPESYVIEADTLAEEGKYIQAVEVYQQAINANPHNLDNYLKI
                     ARLQIFTSQLEQAQVNAQNAILLDNNSSEAYALLGWAKAFQREYLAGEADLRKAVELN
                     QNSGLAHAIYAYVLALRVEARMDDLDTMDRAIEESRTALALEPNLMEAHWARGYVLEV
                     TANFAEAVESLKIANQINPNVDRLHMALGRNLTYIDQLDQAVFEFTKAYSLNPTSPAP
                     NLAISQVYSRLGEFDKAIQYGEVALRDGPTQANLYANLGTLYYRKSQFNTAIEYLRIA
                     VRGGLSEGGLAVEGIPLSYDLGVVEIYSRYALALAKINECNEAVTIAEGMRQTVPDSA
                     VGQENATIIIQICEENLLNPPTPTSAPTPTVVTGPTATPEGTQP"
     gene            complement(4218..5582)
                     /locus_tag="PK791_01375"
     CDS             complement(4218..5582)
                     /locus_tag="PK791_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH91995.1"
                     /translation="MKTTTIINRILLCFIIVAMTFPVLPVDAGNVETVTFVVDTTEDL
                     VDDMLNGECSVGVPSGGPCSLRAAFSEARNIMQMNQNLIIQIPEGIYELTLTEDTGSD
                     RQFGDLDLVSLNAPTERTVTIQGAGALNTIINANDIDRAIQIGAYYNVTINDLRIMRG
                     ELHAANGAGILVENAGKLELNRVKMDYNRAHKVGNNSTSGGAISAFATPLTIRNSSFD
                     NNWADYYSAIKFFGGENVPLKIIASSFRFNKAHNGYTLTSVGPFFMVNSSIISNDEGS
                     GSLALPYEAHIQNSTIVNNSKGVNIGYSDKLYLRNSIILSRPDSNGETGENCEVLLPA
                     KVANNDDFPVIITEGGNVFSDNTCVPDTDLRDIVVPWEEAGVAEIPAYQGILSALPLL
                     PHSPAVNRRFEPCLETVIFWGVPFEVPLMIDQFDRLRGMFCDAGALGLKNKTYIFYPL
                     IERE"
     gene            5956..>7821
                     /locus_tag="PK791_01380"
     CDS             5956..>7821
                     /locus_tag="PK791_01380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOH91996.1"
                     /translation="MMNSQNHDEEKQIPDEESVEQSLGQTGRRPDFDTPKRPDWMREH
                     AQAGDTNLDGYTGFSSIKDSLAQTDNLFDELKDFDYFDVPVVSPGDSVPTFTGNMGDH
                     DLDEMTAQNVPDKAQIPPAEQAIVRPGEYTQSDMDEMRTIPPPAGVPVAPPPASALPQ
                     AVSQLDPYATRLSPTAFQRDLEAASVSTQPVPVQIHQTQPIVAVPPARNVPSAPSGAG
                     KKPKKKGRAGRVILILIILFLLAMFAVAAFAFYKYTQIAKTLPDVNDLQYRASQFETT
                     RILDRKGNVLYEIIDPSAGKRTWVPLDQISPNLIAATIATEDKEFYNHPGFDLVALGR
                     ALWTNYRSKEVVSGASTITQQLARLLLLTDERYEQTYERKAREIILAAEITRRYSKEQ
                     VLEMYLNEIYYGNMSYGVQAASETYFNTNASDLSLWQAAFLAGLPQSPAVYDVYNNRE
                     ATLTRSDSVFRLMFEASTEKGCIKVDEIRPPVCVDAMQVLTESEVLKAYTFKQNLLTM
                     QFPHWVVYIQSLLEAQFDAQMIYRSGFTIHTTLDPDFQREAEAAVREQLAQMQGKNAT
                     NGAVLAINPKTGEILAMVGSADFNNPAISGQVNMVLTQTRQPGSAIKPFTFLAAFE"
BASE COUNT         1895 a         1860 c         2057 g         2009 t
ORIGIN      
        1 ggtgagtatg gcgatgtctt gcaacatggc cttgcggcgg tcgccaaaac caggggcttt
       61 cactgccaca acgttgagca taccgcggat tttgttgaga accagggtag ccaaagcttc
      121 gccgtcgatg tcttcagcaa taatcaaaag ttcgcgtttg ccaatctgca ccattttctc
      181 gagcaggggc acgatgtcgg cagcagcaga aatcttcttg tcgtaaatta agatataggg
      241 gtcttcgatg tcagcaagca tatgctcggg atcggtaacg aagtaggggc tgatgtagcc
      301 acggtcgaac tgcataccgt caacatattc ggtctcaaat tcgaggcctt tggattcttc
      361 aacggtaatc acgccgtctt tgccgacttt gtccataacg tcagcgatga ggtcaccgat
      421 ttgggtgtcc tgggctgaaa cagttgccac agcgccgatt tcttctttgg ttgtgatggc
      481 aatcgcctgt ttgtggatgg cttgagagac gaccttggtg gcagattcga tgccacgttt
      541 gagcagcatg gggtttgcac ccgcggcaag attcttgaga ccctcggtca cgatggcatg
      601 tgccaaaacg gttgaggtgg tggtgccgtc gccagcgatg tcgttggtct tggtggctgc
      661 ttctttgagc agttgggcac ccatgttctc aaaggggttt tcaagttcaa tctctttggc
      721 aacggtgaca ccatcgtggg tgatggtggg cgagccatat ttgcgatcca aagccacgtt
      781 gcgacccttg gggcctaaag tagtggcaac agcgtttgca acagcatcaa cgccactctt
      841 gagatagcgt cgtgcttctt ctccaaaaac taattgttta gccatatata tttctccttg
      901 tgtgtgattg tcttattaaa gaatagcgag cacgtcgctc tcgcgcagaa tgagcaattt
      961 tttaccgtcg tacttgattt cagttccaga atattttgca taaagtacaa cttcaccaac
     1021 cttaacatcc atcgggatgc gttcaccagc gtcgttgcgt tcgccgggtc caatcgctaa
     1081 aacggtccct ttttggggtt tttctttagc ggtttcggga agcacgatac caccagcggt
     1141 gatgtcttct tgttcgattg gttcaataac cagtcggcta cctaatggtc gcattttaat
     1201 tgtcatcgaa ttcctccaat aattaaacta agtttcaaat tttagcactc tcgacttgag
     1261 agtgctaata catgatacgc tttccaggcg cgtaagtcaa gcaatcgtta gcaattctaa
     1321 cagaacgctt acatgcttat ggcgttggcg tgaaggtggg aatgaccaag ggggtttcgg
     1381 tgggttgggg cgtgggtgtg ctgatgtaat ctcgggtggg cacaggcgtg ctaaagacct
     1441 ggccgggcgt gatgccgcag atgttttcgc tggcgctcac gatgcccagc acggtgcggg
     1501 tgaactcgtt ggatgtgtcg taatagcgtt cgcgaatttc cttaaacact tcggaagcca
     1561 gcgcgcaacg gtcatcctgg ccacttctgt gcaggccagc taaaaccgag ccatagatgt
     1621 aataatagac caccgttgag tcggaaaggg gcagcttttc tgtagtgata tcgctttggc
     1681 aggggtcttc ttcgcgtgcc aggcagcttt cttcggcatt gcaacccgta acggcgcatt
     1741 tgagcaccat ctccgcgcct tcatagtttc gggctttgta ataaatgacg cccacttgcc
     1801 cataggtgct ggggttgtag gggttgatgg acagggcacg ataggcgttg cgtgaagcaa
     1861 tcatggcttc acccatgcga atgtaggtgt tggcgatggc catatagggg atggggtctt
     1921 caatgccaag ctgcgagttg aggttggcgg ctttggcgaa gtattcgagg gcaatttcaa
     1981 aattgacatc gccgccattg tgccggtaga gcgtgccaac ccgtatataa agaaaagtga
     2041 gattaggggt aacatcaatc gctttttgat accagtcgat agccaggttg tactcctgca
     2101 acacttcgta gacaaaagcc tgcacacggc gtacgtccat taaatgttcc ccgttttctc
     2161 gcgcctgagc gatgtagtcc tgcgcctgac cccatttgaa ctggtcgatg agcacttcgg
     2221 cgtagtaggc tttggcaagc gcgttgctgc tgtccagctg aatagccgcc agagcgtcgg
     2281 cttcggcggc gttgcgcaat tctttggtgc ggtcgcccgc cagggcgggg ttagccgacc
     2341 agttgagcac aaaggaacga accgccagcg cgtccgcgtt ttggggtgcc agttcgcggg
     2401 cttcatcaac aactgccagc gcgtctgcca ggcggtcgcg tcgttcctgg tcggtggtga
     2461 gcgaggcgga agagtaaacc tgaatgcgcg ccagattcac acgcacatca acattttccg
     2521 gttcaagcaa aatcgctttg ttgtaagaat cgatggcggc gtcaaggttg ccggcctgaa
     2581 agtgggtgtc gccctcaacc aaaaaagaat tgaccgtgcg ggtaggggtg ggggtgggca
     2641 caaaaggcgg ccaaattttc ccgctggtat agtcgcgcat aaccgctatc acaatcacga
     2701 tacacagcaa tataaacatg attaagtatg ggttggacgc cggctttggc ttatgaaata
     2761 agttcttgcg ttcgttgatg tacatagcaa gccagcttta tggttgcgtt ccttctggtg
     2821 tggcggtggg accagtgacc acggtgggtg ttggcgcgga ggttggcgtg ggcgggttga
     2881 gcaggttttc ttcgcagatt tgaatgatga tggtcgcgtt ttcctggcca accgcgctgt
     2941 cgggcacggt ttggcgcatg ccttcggcga tggtgacagc ttcgttgcac tcgttgattt
     3001 ttgccagagc caaagcatag cgactgtaaa tttcaaccac gcccaggtca taggaaagcg
     3061 ggataccttc aacggccaaa ccgccttcag ataagccccc gcggacggcg atgcgcagat
     3121 attcaatggc ggtgttgaat tgcgattttc gatagtaaag cgtgcccagg ttggcgtaaa
     3181 ggttggcctg ggtgggtcca tcgcgcaggg cgacttcgcc gtactgaatg gctttgtcga
     3241 actcacccag tcggctgtaa acctgtgata tggccaggtt cggcgcggga ctggtggggt
     3301 tgagcgagta agcttttgta aattcaaaaa cagcctggtc gagctggtcg atataggtca
     3361 ggttgcgtcc cagggccatg tgcaaacggt ccacgttggg gttgatctgg ttggcaatct
     3421 tcagcgattc cacagcttcg gcaaagttgg cggtgacttc cagcacgtaa ccgcgcgccc
     3481 agtgggcttc catgaggttg ggttccagtg ccaaagcggt gcggctttct tcgatggcgc
     3541 ggtccatggt gtcaaggtcg tccatgcgcg cttccacgcg cagggctagc acgtaagcat
     3601 aaatggcgtg cgccagacca ctgttttggt tgagttctac cgctttgcgc aggtcggctt
     3661 cgccggcgag atattcgcgc tgaaaagcct ttgcccagcc cagcagggcg taggcttctg
     3721 aactgttgtt gtcaagcaaa atcgcgtttt gggcgttcac ctgggcttgt tcaagctgac
     3781 tggtgaaaat ctgcagccgg gcaattttga gatagttgtc aagattgtgt gggttggcat
     3841 taatcgcctg ttggtaaact tccacggctt gaatgtactt gccttcttcg gcaagcgtgt
     3901 ccgcttcgat gacgtaggat tcagggtcgc gtgtgggtgt gggggtgggt acaaagggtg
     3961 ggtttatcat gggtaccaca accacgttca ggtaaaccaa gccggcaata gccaggacta
     4021 aaaacaagat tagccaggga ttaccacgct tgcgtcgcga ctgaattgaa aacttactgc
     4081 ctttaagata cattgcatca attatcatca attatgacgg atgagtcaaa ccagataaaa
     4141 aagtcctgca tgacaaagca gcaggacctt tacattaata tactaaattc tcattaacaa
     4201 caatctcaaa agctaaatca ctcgcgttcg ataagcgggt agaagatata ggttttattt
     4261 ttcaaaccca aagcgcctgc atcacagaac ataccgcgca gcctgtcgaa ttgatctatc
     4321 atgagtggca cttcaaaagg aacgccccaa aaaatcaccg tctccagaca gggctcaaaa
     4381 cggcggttga ctgctggtga atgaggcaga agaggtaatg cagataatat tccttgatag
     4441 gcaggtattt cggcaacgcc tgcttcctcc cagggcacaa caatatccct caaatctgtg
     4501 tctggcacgc aagtgttgtc agaaaaaacg ttaccacctt cagtaataat cacagggaaa
     4561 tcatcattgt ttgccacctt ggcaggtagt aatacctcac agttttctcc agtttcacca
     4621 ttggaatcgg gtcttgaaag gataatactg ttgcggagat agagcttgtc ggaatagccg
     4681 atattaactc ccttgctatt attcacgatg gttgaatttt gaatgtgggc ttcatatgga
     4741 agtgccaaac tgccactacc ttcatcgttt gagattatag aagagttaac catgaaaaat
     4801 ggtccgactg aagtcaaagt gtaaccattg tgcgctttgt taaagcgaaa agaggatgcg
     4861 attatcttca aaggcacatt ttccccgcca aaaaatttga ttgcggagta ataatctgcc
     4921 cagttattgt caaaactgct attccgaatg gtcaaaggtg ttgcaaacgc gcttatagcg
     4981 ccaccggagg tggaattatt accaacttta tgcgctcggt tgtaatccat tttcacacga
     5041 tttaactcaa gtttcccggc gttttcaacc aaaatacccg cgccattagc ggcatgcaat
     5101 tctccacgca ttattctcaa atcattgatg gttacattat agtatgcacc tatctgaatc
     5161 gcccggtcga tatcattggc attgatgatt gtattcaacg cgcctgctcc ttgaatggtg
     5221 actgtgcgtt ctgtaggcgc atttaacgac accaaatcca aatcgccaaa ttgacgatcg
     5281 cttccggtat cctcggtgag tgttaactcg taaatgcctt cgggtatctg gataatcagg
     5341 ttttgattca tctgcatgat atttctggct tcagagaacg cggcgcgcaa agagcaggga
     5401 cctcccgatg gaacgcccac actgcattcg ccattgagca tatcgtcaac cagatcttcg
     5461 gttgtgtcca ctacaaatgt gaccgtctct acattgccag catcaaccgg caataccggg
     5521 aacgtcatcg cgacgatgat gaaacagagt aatattcgat taatgatggt tgttgttttc
     5581 attagacctc cgatgggtat taactaattt ggaataaaga cgtaccctgc tcagatgaac
     5641 aaagcaccag caaaaaattg ctgagaatgc actattgttg gttctttggg tcttgaaaag
     5701 cccctttttt agtaattgta aacggctctc aaatatatgt caatcttttt cttttatgaa
     5761 attaatgcat taaattctaa ggaggcattt tccttcatac ttatgggggg gaacgcagtg
     5821 attgacgggt aacacattaa tcaacaaacc tgttcgcttt tcagtatctt aacagaagaa
     5881 gtatgcaaca tgcaggcaat atatgccttt aagcgtttat gctaaaatga gagtaataat
     5941 aaactttggt gatggatgat gaattcgcaa aaccatgatg aagaaaaaca aatacctgat
     6001 gaagagtcag ttgagcagtc tttggggcaa accgggcgaa gacctgattt tgatacccct
     6061 aaacgtccgg attggatgcg tgaacacgcg caagcaggcg acactaacct tgatggctat
     6121 accggtttta gctcgataaa agacagtctc gcgcagacgg ataatctctt tgacgaactc
     6181 aaggattttg attattttga tgtccctgtt gtttcaccgg gtgattccgt gcccacattt
     6241 acagggaaca tgggcgatca tgaccttgat gaaatgaccg cgcaaaacgt gcccgataag
     6301 gcgcaaattc cacccgcgga acaagccatc gttagaccag gcgaatatac ccaaagcgat
     6361 atggacgaga tgcgcaccat cccgccacca gcgggtgtgc ccgtcgcgcc accgccagcg
     6421 agcgctttgc cacaagctgt gagccagctg gatccctacg cgacgcgtct aagcccaaca
     6481 gcttttcagc gcgatttgga agctgccagt gtgtctaccc agcctgtgcc tgttcaaatt
     6541 catcaaaccc agcccatcgt tgctgttcca ccagcgcgaa acgtgccatc tgcaccgagt
     6601 ggcgcgggta aaaagcctaa gaaaaagggc agggcgggac gcgttattct tatcttgatt
     6661 attcttttcc tgttggccat gttcgcggta gcggcttttg cgttttacaa atatacgcaa
     6721 attgccaaga ccttgcccga cgtgaacgac ttgcagtatc gggcttctca gtttgaaacc
     6781 acgcgtattt tggaccgcaa aggcaatgtg ctttatgaaa ttatcgaccc cagcgcgggc
     6841 aagcgtacct gggtgcccct ggaccaaatt tcgcccaact taatcgccgc taccatcgcc
     6901 acggaagata aagagttcta caatcatccc ggttttgact tggtcgcact ggggcgggcc
     6961 ttgtggacga actatcgctc aaaggaagtc gtttccggcg cttcaaccat cactcagcaa
     7021 ctggcgcgtc tgttgctgct taccgatgag cgctatgaac agacttacga acgaaaagcg
     7081 cgcgagataa ttctggcggc agaaatcacc cgccgttaca gtaaggagca ggtgcttgag
     7141 atgtacctga atgaaattta ctatggcaat atgtcttatg gcgtgcaagc cgcgtcggaa
     7201 acctatttca acacgaatgc ttctgatttg agcctgtggc aggctgcttt tttggcgggg
     7261 ttgccacaaa gcccggcggt gtatgacgtt tataacaacc gcgaagcaac gcttacccga
     7321 agcgactcag tctttcggct gatgtttgag gccagcacag aaaagggttg tattaaggtg
     7381 gacgaaatcc gcccgccggt ctgtgtggac gccatgcaag tgcttaccga atcggaagtt
     7441 ttgaaagcct acacatttaa acaaaacctg cttaccatgc agttcccgca ctgggtggtg
     7501 tatatccagt ccttgcttga ggcgcagttt gacgcgcaga tgatttatcg ttcgggcttt
     7561 accattcaca ccacgcttga ccccgacttc cagcgcgaag ctgaagccgc cgtgcgtgag
     7621 caactggccc aaatgcaggg caaaaacgcg acaaacggtg cggttttggc gataaatccc
     7681 aagacaggtg aaattttggc gatggtcggt tcggcggact ttaataatcc agcgattagc
     7741 gggcaagtga atatggtatt aacccaaacg cgtcaacctg gttcggcgat taagcctttc
     7801 actttcctgg cagcatttga a
//
LOCUS       DAOSFC010000027         7777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_8099_length_7777_cov_7.620824, whole genome shotgun sequence.
ACCESSION   DAOSFC010000027 DAOSFC010000000
VERSION     DAOSFC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8099_length_7777_cov_7.620824"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK791_01385"
     CDS             complement(<1..608)
                     /locus_tag="PK791_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH91997.1"
                     /translation="MPRSIRSTEREKKKNVWLWLVLVLLACVVLSVATCFIFRVHIKK
                     VLEQEIVRVWLAFALVLVFYLLLGLIAILLVLRHFNKERLLKEEQAPSPKPIIKRVVS
                     AKKASISDVAELDSMIQSGLAQFDSQLESQKQFIADAIHELNTPTALLRLSIDSYRKR
                     LQDGDAKIFDYVFMDTLNHATQRIENLVEQIDKLDAKPIAEA"
     gene            complement(639..1322)
                     /locus_tag="PK791_01390"
     CDS             complement(639..1322)
                     /locus_tag="PK791_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH91998.1"
                     /translation="MRILLIEDEEYFANMLANVLRQESYIVDIAFDGEMGIYHALESQ
                     IDYDLILLDLGLPGVDGMTVCKQIRDSKPHARILILSARGKTFDRVMGLDIGADDYLT
                     KPFAIEELLARIRALLRRTVQGTSPMLSLGALSLDPARREAFVNGKSVSLTGTEYVLL
                     ENLMRYPGQVISQETLINKSWDDFGRTLFSTTLRTHIYNLRRKLRRANLKDVAIKTVT
                     NQGYQISLE"
     gene            complement(1429..4587)
                     /locus_tag="PK791_01395"
     CDS             complement(1429..4587)
                     /locus_tag="PK791_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH91999.1"
                     /translation="MKTKSQNVFWLSFFRRPLMSLLILLAIGFVSFGFVGKAVETIIV
                     WRETNRLEGYYRSIGAIYKDFEGEEHLFSEGAAIIEQSPAFAFGDFKRQTAGFMQDIY
                     NVDFDTGTMDVGETLYADASMWYGEGVYNLDYWFYGTLVDSQILKTNKKPVLTSGYLL
                     VFEVDEVLAGYPERVQAGKRYVVWVPVNYTIGFEKIEVHLKEMRLGQRYLIRAWSHPT
                     FPFSVTGVNAVNLNDAFNLKALDDKDLWYIPAEKDESIDLSLSKYEGIRLEIDRLNEN
                     LRSILLIGTSDMSALPEVQLDSKYFFLVEGRWLNREDEEQAKPVIVIAKNLAETRGIG
                     IGNQITFTLRALKDPYHSYIRGEEDIRHWRDYPSKTVSYEVVGIYSHPGFEQSYFKQN
                     WFSNAYVPNATLPDEFIHTMSWSAVGDKTLSYSFVLTDPRLQDAFLEEYSPKLKALGF
                     DLSFVENNAKNFVAGADPLRKSLLMGAVLFAVALLMAIVLSVFLYLRQQRRNYAVLRA
                     LGVPARISNKQLILPLLGLGLLGSVIGALFSWQNAHNRAAESLSKLPLPSGVLPELSL
                     HPALGVGIWFLVLLMIVLTAWLGNRKVTSAPVLELLQDNIAKKQKRVVKDLKAENLTF
                     NKNLISKITQTPSRGKSDSKRALSGFYRSNLLRSPVKSLLTVVVAATLLLALGWFQTL
                     IESNKLEVDRLYKSFQVQIDFFIKSENMLNLISRKGVDWVEQSGVMDHSYLSTLMQTR
                     FELNMETGSFEPMPPYRILALNHLEHGLERSIKGMTINFLPGYGPESFTEKWLESEEG
                     LTKLPLVIPEEILSAEGWQLGDELIWEFFEFDKPVTFVVVGSSVGGSTVYGAMGRNGW
                     QFGPYSYMITNLSAIETYFSTPPNYAEVAFYSAPEMNYQLKAFKSELLAKQEETTWFP
                     ATIHFWDEELLAVVEPMEQNLSLMERLYPVTLLLAGSIGGVLCLLLVLNQAKETALLR
                     MLGVEKRQIRRMQMSQIVLLSVIGLIVGLVVLIVLRTTAAARWSLGVAGGIYLAGAFL
                     GALVGGILVAGKKPMELLQVKE"
     gene            complement(4642..5310)
                     /locus_tag="PK791_01400"
     CDS             complement(4642..5310)
                     /locus_tag="PK791_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92000.1"
                     /translation="MNKLTLENVSYAYKGAERKALDDISVEFQGGKIYAVIGPSGSGK
                     TTLLSILAGLDIPTEGEVFINNTKLSTLDLDQYRREQVSMIFQAFHLFPMLNVVENVS
                     FPMELNGVERAEARTRAKVFLNSVGITDEKHRRFPSNLSGGEQQRVAIARTMASGARV
                     ILADEPTGNLDKENGQNIVDILKVLAHENGYCVIIVTHDPAIAEQADQVYAMDDGRMT
                     EARD"
     gene            5572..5919
                     /locus_tag="PK791_01405"
     CDS             5572..5919
                     /locus_tag="PK791_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HOH92001.1"
                     /translation="MENKELLNKLRAMQTLYARIDQDNKAIEAFTQFLIESKQRCDAL
                     SEFYENEWLDVVGEDERRVEKGLPSLGIVVYESSEPNGKYSILGEDTIWNTLSENRQI
                     LLELLKTLVPMIS"
     gene            6193..6381
                     /gene="rpsU"
                     /locus_tag="PK791_01410"
     CDS             6193..6381
                     /gene="rpsU"
                     /locus_tag="PK791_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOH92002.1"
                     /translation="MASVSLRQNESQDSLLKRFRKKVVKSGVLSTVRRKRWFVSKSEQ
                     RRMDKKKAVRRNFRKSYD"
     gene            6405..6659
                     /gene="infA"
                     /locus_tag="PK791_01415"
     CDS             6405..6659
                     /gene="infA"
                     /locus_tag="PK791_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOH92003.1"
                     /translation="MAREDEKITVEGTIIEALPGTQFRVKLENGHEVIAYLSGRMRRY
                     YIRILLGDLVRVEMSPYDLDRGRIVYRQRRTGPIDPTDFS"
     gene            6818..7777
                     /locus_tag="PK791_01420"
     CDS             6818..7777
                     /locus_tag="PK791_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH92004.1"
                     /translation="MDPSRDLDAVANLIETAFELKNDPEGMNVVHNMRQHARMLASAD
                     ATFLDNLSVKASYSNGLVWEDQGQVVGNITLIPHTDGFRRVIMIANVSVQDDYRRKGI
                     ARELTIKALRHCRSLGVRGVFLQVRHNNQGAIQLYRDLGFHFLHSVSVWRLRPSPSGL
                     DELGKRGAELESGFSIRKRRPPDWDKQKEWLDALYPQKTRWYAGLVARHLSPLAWLNP
                     FAWPQVSQTEHFALRYAKTLAGVLTFQRRSPQIDQAWLALPPGLDPKDENQRASMLLR
                     HFVRERWNHGYLNMDFPLGHATQGIQEAGFTLAHDLDWMAIRL"
BASE COUNT         2080 a         1992 c         1751 g         1954 t
ORIGIN      
        1 tttgcttccg cgataggctt cgcgtcaagt ttatcgattt gttcaaccaa attttcaatg
       61 cgttgggtcg catgattgag ggtgtccatg aaaacatagt cgaaaatttt cgcgtcccca
      121 tcctgtaaac gttttcggta actgtcgatg gacaagcgca acagggcggt gggcgtgtta
      181 agctcatgaa tcgcgtcagc gataaactgt ttttgacttt ccagctggct gtcaaattgc
      241 gccaggccgg attgaatcat cgaatcaagt tccgccacat cgcttattga agcttttttg
      301 gcgcttacga cacgcttgat tatcggtttg ggtgaaggtg cctgttcttc tttaagcaat
      361 ctttccttat taaaatggcg caaaacaagc aaaatcgcaa tcaaaccaag taagagataa
      421 aacaccaaaa ccaaagcaaa tgccagccaa accctcacga tttcttgttc cagcaccttc
      481 ttgatatgaa cgcgaaaaat aaaacaggtg gcaacagaca aaaccacaca agcaagcaaa
      541 accagtacca gccaaagcca gacattcttc tttttttctc tttcagtgct tcttatactt
      601 ctcggcatgg tttcgagtac cccaaacatg aactcgtctt attcaaggct tatttgatag
      661 ccttgatttg taaccgtttt aatcgctacg tctttcaggt tggcacggcg caattttcgt
      721 ctcaggttat agatatgtgt tctcagcgtt gttgaaaaca gggtacggcc aaagtcatcc
      781 cagcttttat tgattaatgt ttcctgactg attacctgcc ccggataacg cataagattc
      841 tccagtagaa catactccgt gccggtaagg ctgacacttt ttccattcac aaaagcttca
      901 cgcctggcgg ggtctaacga caacgcccct aaactgagca tgggtgatgt gccctgcaca
      961 gttcggcgca gcaatgcccg aatgcgtgcc agcaattctt caatcgcgaa aggtttggtc
     1021 aggtaatcat ccgcgccgat atccagcccc atcacacggt cgaaggtttt tccgcgggcg
     1081 gacagaataa gaatacgcgc atggggttta gagtcgcgaa tttgtttgca aacggtcatg
     1141 ccatccacgc ccggcaaacc caaatccagc agaataaggt cgtagtctat ctggctttca
     1201 agggcatgat aaatccccat ctcgccgtca aaagcaatgt ccacaatgta agactcttgc
     1261 cttagcacat tagcgagcat attcgcgaag tattcttcat cttctatgag caggattcgc
     1321 atagccttta accttaggat aattcagctt tgtttatatt ttatcaccgg tagacaagac
     1381 gggatttcta ttgtaggcaa gcaagccaaa ataaacagct ctaagtcctc actctttcac
     1441 ctgtagaagt tccataggct ttttacccgc caccaggatg ccacccacca gcgcgcctaa
     1501 aaatgcccca gcaaggtaga ttccgcccgc gactcccagc gaccaacgcg ctgctgcggt
     1561 agttctcagc acaatgagaa ccaccagacc cacaatcaaa ccaatcacac tcaagagaac
     1621 aatttggctc atctgcatcc ttcttatttg gcgtttctcc acgcccaaca tacgcaaaag
     1681 cgcggtttct ttcgcttgat tcagcactaa aagcagacag agcactccac cgatagatcc
     1741 cgcgagcaag agcgtgaccg ggtaaagccg ttccatcagc gaaagatttt gttccatcgg
     1801 ctcaaccacc gccaaaagtt cctcgtccca aaaatgaatc gtcgcgggaa accaggtggt
     1861 ttcttcctgt tttgctaaca actcagattt aaaggctttc aattgataat tcatctccgg
     1921 cgcagagtaa aaagcaactt cagcataatt tggtggggta gaaaaatagg tctcaatggc
     1981 agaaaggttc gtgatcatat aactataggg gccaaactgc caaccattac gtcccattgc
     2041 accataaaca gtgcttcctc ccacagagct tccaaccacg acaaaggtta ccggtttgtc
     2101 aaattcaaaa aattcccaaa tcagttcatc gcccagctgc cagccttcag ccgacagaat
     2161 ttcctcagga atcaccaagg ggagtttcgt tagcccttct tcgctttcca gccatttttc
     2221 agtaaaggat tccggtccat accccggtag aaaattaatg gtcatcccct taatagatct
     2281 ttcaagcccg tgttccaaat ggtttagcgc aaggattctg tatggcggca taggctcaaa
     2341 ggaaccggtt tccatgttca gttcaaaacg tgtttgcatc agagtggaaa gatagctatg
     2401 atccatcaca ccgctttgct ctacccagtc gacgccttta cgagaaatca ggttcaacat
     2461 attctcactt tttatgaaaa agtcaatttg cacctgaaaa cttttataca gacggtctac
     2521 ctccagcttg ttggattcga tgagcgtttg gaaccaccct aacgccaata ataaagtcgc
     2581 cgcgacaaca accgtaagca aacttttcac cggggatcgt aaaaggttcg agcgataaaa
     2641 gccggataaa gcccgtttcg aatcggactt accccgcgaa ggcgtttggg taatcttgct
     2701 gatcaggttt ttgttgaaag ttagattttc agcttttaaa tctttgacaa cgcgtttttg
     2761 ttttttagcg atattatcct gaagcagttc caaaactggc gcgctggtaa cctttcggtt
     2821 gcccagccag gcagtcaaaa caatcatgag caaaaccagg aaccagattc ccaccccaag
     2881 cgccggatgc aggcttagct ccggcaacac accagatggc aagggcagtt tggaaagcga
     2941 ttcagccgcc ctgttgtgcg cgttttgcca ggagaacagg gcaccaatga ctgaacccag
     3001 caaaccaagc cccaacaagg gcaagatcaa ttgtttgttg ctaattctgg caggcacccc
     3061 gagcgcgcgc aggacagcat agttgcgccg ctgctggcgc agataaagga aaaccgacaa
     3121 aacaatcgcc atcagcaaag caaccgcgaa gagaactgca cccattaata aagacttacg
     3181 caaagggtcc gcccccgcga caaagttttt ggcgttgttt tcaacaaaag acaagtcaaa
     3241 acccagtgct tttagttttg ggctgtactc ttcgaggaaa gcatcctgca aacgcgggtc
     3301 agtcaacacg aagctatatg acagggtctt atcgccgacg gcagaccaac tcattgtatg
     3361 aataaattcg tccggtaagg ttgcgttggg gacataagcg ttcgaaaacc agttttgttt
     3421 aaagtaggat tgttcaaacc cgggatgtga ataaatgccc acgacctcat agctgaccgt
     3481 cttgctggga taatcgcgcc agtgccggat atcttcctca ccacgaatat aactgtgata
     3541 ggggtctttt aatgccctca gggtaaaagt gatttgattg cctatgccaa ttcccctggt
     3601 ttcagccagg ttttttgcaa taacaatcac cggtttggct tgctcttcat cttcccggtt
     3661 caaccacctg ccctccacca ggaaaaaata tttcgaatcc agttgcactt caggcaaagc
     3721 actcatatcg cttgtgccaa tcagtaaaat cgacctgagg ttttcattga gccggtcaat
     3781 ttcaagccgt attccttcat acttggacaa ggaaaggtca atgctttcat ctttttcggc
     3841 cggtatatac cataaatcct tgtcatccag tgctttcaga ttgaaagcat cgttcaaatt
     3901 gacagcgtta accccagtaa ctgaaaatgg aaaagtcgga tgagaccatg cccttatcaa
     3961 atagcgttgc cccagtcgca tttctttcag atggacttct attttttcaa acccaatcgt
     4021 gtaatttact ggaacccaga cgacatatct tttacctgcc tggacacgct ccggataacc
     4081 tgccaggact tcatccactt caaaaacaag caaatagcca ctggtaagaa caggcttctt
     4141 attcgttttc agaatctgac tatcaaccag agttccgtaa aaccagtaat ccagattata
     4201 aacgccttcg ccataccaca tactggcatc cgcatacagg gtttcaccca cgtccatggt
     4261 accggtatca aaatctacgt tgtagatatc ctgcataaag cccgcggttt gccttttaaa
     4321 atcaccaaac gcgaaagccg ggctttgctc gattatcgcc gctccctcag aaaataaatg
     4381 ttcttcccct tcaaaatcct tgtaaatggc accaatcgac cgataataac cctccagccg
     4441 attggtctcg cgccaaacaa tgatggtttc aaccgccttg cccacaaagc caaaggatac
     4501 aaagccaata gccaaaagga taagcaaact catcaatggc cgacgaaaaa atgagagcca
     4561 gaatacattc tgcgatttgg ttttcatctt gtttcccttt ctgcaaaaca gcgctttgtt
     4621 tcaggaatag ttttagcgaa actaatcacg cgcttccgtc atccgcccgt catccatcgc
     4681 gtaaacctgg tcggcttgtt cagcgatggc ggggtcatgg gtgacgatga tgacgcagta
     4741 gccgttctcg tgtgccagca ccttgagaat atcaacgata ttctgcccgt tctccttgtc
     4801 gaggttccca gtcggttcat cagcgagaat gacacgcgcg ccactcgcca tggtgcgcgc
     4861 gatggccacc cgttgctgtt caccgcccga aaggtttgag gggaagcgtc tgtgtttttc
     4921 atccgtgatg ccaaccgagt tgagaaatac cttcgcccgg gttctggctt cagcgcgttc
     4981 caccccgttc agctccatcg ggaaactgac gttttcaacc acattcaaca tcggaaacag
     5041 atgaaaggcc tggaaaatca tgctgacctg ctcacgacga tattggtcga ggtccagcgt
     5101 ggaaagcttg gtgttgttga tgaacacttc gccttcagtg gggatatcaa ggccagccag
     5161 gatggataac aaagtagttt taccagaacc acttggacca ataacggcgt aaattttacc
     5221 gccctgaaat tcgacactga tatcatcaag cgctttgcgc tccgcgcctt tgtaggcgta
     5281 gctaacgttt tcaagggtaa gtttgttcat atggcctcca attaaagaga tgaaatctat
     5341 aaatcaatac aggtatttgt tgttttatca cgtgaatcat accagtcaag atgtcaagat
     5401 tttgttaaga aaaatgtttt tcagttggtt tgcctgaaag caaaggctct cattttaaaa
     5461 gtgagtttgg caaagcaatt tctatcaggg aactttatgg cacggcaata cgtataatta
     5521 gggcgattaa tatgcgttct tcgcaaaatg agttaagcga ctggagaaac gatggaaaac
     5581 aaagagcttt taaataaact gcgcgcgatg cagaccctgt acgcgcgaat cgaccaggac
     5641 aacaaagcca ttgaagcctt cacacagttt ttaattgaat ccaaacagcg ttgcgacgcg
     5701 ctttctgagt tctacgaaaa cgaatggctg gatgtcgtgg gagaagatga gcgacgcgtt
     5761 gaaaagggtt tgcccagcct gggcatagta gtttacgagt ccagtgagcc gaacgggaaa
     5821 tactccatcc ttggcgaaga cacgatttgg aacacactca gcgaaaaccg ccaaattttg
     5881 cttgaacttc tcaaaacgct cgtgccgatg ataagctaac ttttcaagca gcgtctgttc
     5941 gcccgtcatc tgccggccac gggcgatgtt tgtcgccata gcttgatact ggtgcctgct
     6001 tgtccatcgc gtgtttttcc caggcggtgt tgtaaacaca attttatccg cgcctggcaa
     6061 aatccgattc aaatacgttt gatttttacc aaattcgtgc taggtttttt tagacatttc
     6121 gtgtataatc gctagcacgt ctgattaacg tgtattttga cctattctat gcccggaagg
     6181 aggtgaatag caatggcaag tgtcagttta agacaaaatg aatcgcaaga ttctctactc
     6241 aagcgctttc gcaagaaagt cgtcaagagt ggcgtattaa gcacggtgcg tcgcaaacgc
     6301 tggttcgttt caaagagcga gcaacggcgc atggataaga aaaaggctgt tcgtcgtaac
     6361 ttccgaaagt cctacgatta gccgagagta gtaagtgagg ttttatggct agagaagatg
     6421 agaaaattac agtagaaggc accatcatcg aagccttgcc cggcacgcaa ttccgggtga
     6481 agctcgagaa cggccatgag gtcatcgcct atctttcggg acggatgcgc cgatactata
     6541 tccgcattct cctgggcgat ttggtgcgcg tggaaatgtc tccttatgac ctcgaccgtg
     6601 gcaggattgt gtaccgccaa cgccgaactg gcccaatcga ccccacggat ttttcgtaag
     6661 catgttttat cgcccaggac attgcgccag ggctaaagca tacccaacac aaagcttcca
     6721 tcatgtatgg gagctttttt tattcaatat gggcatcttg cattaaaatc aagatatgtc
     6781 atcatcacct tcacgaccgc caggatttgt ccgccgcatg gatccaagcc gcgaccttga
     6841 cgctgtggca aatctgattg aaacggcgtt tgaactcaaa aacgaccccg aaggcatgaa
     6901 tgtcgtgcac aatatgcgcc agcacgcgcg tatgcttgca agcgcggacg ccacattcct
     6961 ggacaatctc agcgtaaaag cctcctattc caacggtctg gtctgggaag atcagggaca
     7021 agtggtgggc aacatcacgc tcattcccca cacggacggc ttcaggcgcg ttatcatgat
     7081 tgcgaacgtt tccgtgcagg atgattaccg acgcaaaggc atcgcccgcg agctgaccat
     7141 caaagctttg cggcattgtc gctcgcttgg cgtgcgcggt gttttcctgc aagttcgcca
     7201 caacaaccag ggcgccattc agctttaccg cgatttgggc tttcactttt tacatagcgt
     7261 aagcgtttgg cggttgcgac ccagccctag tggtttggat gaacttggca aacgtggcgc
     7321 ggagcttgaa agcggtttta gcatccgcaa acggcgcccg ccggactggg ataaacaaaa
     7381 ggaatggctt gacgcgctct atccacaaaa aacgcgctgg tatgcggggc tggtggcgcg
     7441 ccatctctcg ccattagcct ggctgaaccc tttcgcctgg ccacaagtca gccaaaccga
     7501 gcatttcgcg ctgcgctatg caaaaaccct ggctggcgtt ttgaccttcc agcgtcgcag
     7561 tccgcaaatt gaccaggcct ggctggcact gccccccggc ttagacccca aagatgaaaa
     7621 ccagcgggcg tccatgcttt tgcgacactt cgtcagggaa cgctggaacc atggctatct
     7681 caacatggat ttcccgctgg gccacgccac gcagggcatt caggaagctg gctttacctt
     7741 agcccacgac ctggactgga tggcgataag gctttag
//
LOCUS       DAOSFC010000028         7686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_8209_length_7686_cov_5.333377, whole genome shotgun sequence.
ACCESSION   DAOSFC010000028 DAOSFC010000000
VERSION     DAOSFC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8209_length_7686_cov_5.333377"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /gene="argS"
                     /locus_tag="PK791_01425"
     CDS             <1..1051
                     /gene="argS"
                     /locus_tag="PK791_01425"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOH92005.1"
                     /translation="KTRQWSMDGFKQVYELLDAKFDRVYFESDVEESGKVLVEDLLAR
                     GLAEDGRPENPVIIDLDKILGTKEEYRVAVILRSDGTSLYATKELPLAIMKFEEYPLD
                     QSIYVIDVRQSLHMKQMRKVLELMGYDWAEKIYHLAYEIVNLPGNVTMSSRDGTVVLL
                     DDLIREATDRALSIVKEKNPDLSAESQHEIAEMVAIGAIKYPMISRENTKIVTFDWET
                     ALDFNGQSAPYIQYACVRANSILKRAEVNTQYGQTPTASFDYELDAAEVNLIELIAEL
                     PLELSKAAKEYKPSQIATYAYDLAKAFNDFYNQCPVLNAEDEIRTARLALTNAARITI
                     QNALHLLGIKAPQVM"
     gene            1103..2443
                     /locus_tag="PK791_01430"
     CDS             1103..2443
                     /locus_tag="PK791_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOH92006.1"
                     /translation="MSRIRTIIMGAAGRDFHNFNTFYRGNDKYEVVAFTATQIPGIED
                     RRYPAVLAGELYPEGIPILAEEDLAELIEKYEVDEVCFAYSDVPYDYVMSKAAIVNAL
                     GADFRMLGTRETQLESTKPVVAVCATRTGCGKSQTSRRVVADLQDLGYEVVAIRHPMP
                     YGDLAKQAVQRFGELADLEKHECTIEEREEYEPHIENGVVIYSGVDYEAILREAEKEA
                     DIIVWDGGNNDMPFLKPDLLIVVTDPHRAGDELAYYPAEVNVRSADVFVINKVDTADP
                     ELVEFVRRNLLDTNPEAIIVDAASPIFVEDPFAIRGKRVLAIDEGPTITHGDLPYDFG
                     YVAAQRYGAGEIVDPRPFAVGDLKETFENFPHLANVLPSMGYGEKMIRDLEATIEASD
                     VDMVVSGTPIDLSRVMKINKPLVRVTYELQEIGFPTLFDVLEEEFGWKDEEDEE"
     gene            2711..3973
                     /gene="obgE"
                     /locus_tag="PK791_01435"
     CDS             2711..3973
                     /gene="obgE"
                     /locus_tag="PK791_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOH92007.1"
                     /translation="MFVDSVVIWVASGKGGDGIVHMRREKYRAKGGPDGGDGGRGGDI
                     IFEVLPTMNTLNRFHYNERFQADSGKPGGPNNRSGKSAADLTIHVPPGTLIYNDETTE
                     LIGDLVTPGQKLIVAKGGRGGRGNQHFATSRQQVPLTAEKGEPGEEFTLRLELKLIAD
                     VGLVGMPNAGKSSILASTTRAKPKIANYPFTTLFPNLGVVELDLDNTMVLADIPGLIE
                     GASEGVGLGHDFLRHVQRTRVLIHVLDGMSEDPLQDFEVINSELEFFDEHLSRSPQVV
                     VLNKMDLPDVQEKFESIAKNLQEQGIELMPVSAATGLNMKELMWKAYQVLQETPLEET
                     QEELPVYRADVDPRAFEIEQIEEDTWLVTGKMVEKAAAMTYFDQVGSVRRFQRLMERL
                     GVEKALRNDGIQEGDTVVVGEWELEWWD"
     gene            complement(4252..6867)
                     /locus_tag="PK791_01440"
     CDS             complement(4252..6867)
                     /locus_tag="PK791_01440"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOH92008.1"
                     /translation="MTEATPKHGLLPTPPKPEGLPSIELSENSIKILQKRYLRRGLDG
                     EPVEEIDDMFWRVAWHIADVDSSDEEKHALAIQIYHLLVSKAFFPNSPTFTGAGTPLG
                     QLAACFVLPISDDMGRASDGIFSTLRNAALIQQTGGGNGFNFSRLRPSGSTVNSSAGE
                     ATGPVGFLKVYDNAFGEIAQGGTRRGANMGILRVDHPDVEKFITCKTSETAITNFNIS
                     LAITDEFMEAVKNDADWDLVFPDVHDPSYKKFSGTIEDAREQGLPIKTFKTVKARALF
                     DSLVRQAHHNGEPGVLFIDAANRDNPVPHLYHLEATNPCGEQWLGPYENCCLGSINLA
                     EHCGENGSIDWEKLKESTILATKFLDNVVDKNAYVPAVPELRESALNARRIGLGIMGL
                     ADLMYHAGVRYGSEAALDFCGQIMEYIRYYSMLTSIQLAEQKGAFPAIKGSIYDPENL
                     KWQAPTPLKPYTSDWGRPAINWDDIIQGIRQHGIRNAAQTTIAPTGTIGTVAGIEGYG
                     CEPVFALAYMRHVDDRGKKLTLTYVSPHFQNALDKTDLSEESKAEIIEKVLENGNCQD
                     IEELPAHIRDVFVVSADITADEHIHTQAALQRFVDNSLSKTINFPAGTTEEEVADAFK
                     QAWELGCKGITVYITGSRDVVVLETKATSEAKDAPAAAPATPAVQQTLLYGEDLPAQL
                     SFWQDSKKPRPRALQGYTFSIGTPLGKAFVTINENGGGQPFEVFINTSKAGSETAAHS
                     EAIGRLISYILRIASPIQPRERLRIVMQQLGNIGGGRSLGFGSNRVRSLPDGISKALD
                     EYLCQEHFEQEPRPIYMPPQEALPMTQTQVPESHSSPGNPFQRVGELCPECGEATLIN
                     EEGCRKCYTCGHSEC"
     gene            complement(6885..7340)
                     /gene="nrdR"
                     /locus_tag="PK791_01445"
     CDS             complement(6885..7340)
                     /gene="nrdR"
                     /locus_tag="PK791_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOH92009.1"
                     /translation="MKCPHCQHNDSSVVDTNKEAQGGIRRRRECLNCGQRFSTIERPI
                     LTTPLIIKRDGTRETFDREKLLAGLRKASSKRPVTATMIEKLVDQIEAELQKRGRSEI
                     NSRVIGDLAVKGLKELDHIAYIRYAIVYLRMDDLHTVRDEIDHLLSEEE"
     gene            complement(7477..>7686)
                     /locus_tag="PK791_01450"
     CDS             complement(7477..>7686)
                     /locus_tag="PK791_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92010.1"
                     /translation="ASQIVLSRDEKGRFCTFVFKEKFYLLLCLQDFLIMPLPPVKPPK
                     KQENLCKINIHPRETAKLRARFRQC"
BASE COUNT         1884 a         1758 c         2115 g         1929 t
ORIGIN      
        1 gaaaacacgc cagtggtcaa tggatggttt caagcaagtg tatgaattgc ttgacgcgaa
       61 gtttgaccgc gtttactttg aaagcgatgt tgaagaaagt ggtaaggttc tggttgaaga
      121 cttgctcgcg cggggcctgg ctgaagacgg tcgccctgaa aacccggtca ttatcgacct
      181 ggataaaatc ctgggaacca aagaagaata tcgcgtggca gtcatcttgc gttcggatgg
      241 cacgtcgctt tacgccacca aagagctgcc tttggcgatt atgaagttcg aagaataccc
      301 cctggaccag tccatttatg tcattgatgt gcgccaaagc ctgcacatga agcaaatgcg
      361 caaagtgctt gaactgatgg gctatgactg ggcggagaaa atttaccacc tggcttacga
      421 aattgtgaac ctgccaggca acgtaacgat gtcgtcgcgt gatggcacgg tggtcttgct
      481 ggatgacctg attcgcgaag cgacggatcg tgccctgagc atcgtgaaag agaaaaaccc
      541 tgaccttagc gcggaaagcc agcacgagat tgcggaaatg gtcgcgatag gcgcgattaa
      601 atacccgatg atttcacggg aaaacacgaa aatcgtgact tttgactggg aaactgccct
      661 agacttcaac gggcaaagcg cgccatacat tcaatatgct tgtgtgcgtg ccaacagcat
      721 cctgaaacgc gccgaagtga acacgcaata tggtcaaaca ccgacagcgt cctttgacta
      781 tgaacttgac gcagctgaag tgaatctcat tgagctaatc gcggaattac ccctggaact
      841 cagcaaggcc gccaaggagt ataagccttc gcaaattgcc acctatgcct atgaccttgc
      901 caaagcgttc aacgattttt acaaccagtg cccggttttg aacgccgaag acgaaataag
      961 aacagcgcga ctggcgttaa ccaacgccgc gcgcatcaca atacaaaacg cattacattt
     1021 gttgggcatt aaagccccac aagtgatgta aaagtttgtt agaatagtag agtaaattaa
     1081 acctaaacct aaaggagaaa ctatgagtcg tatacgtaca atcatcatgg gagccgcagg
     1141 acgcgatttc cacaacttca acacctttta tcgtggcaac gataagtacg aggtggttgc
     1201 tttcaccgct acccaaattc cgggtatcga ggatcgacgc tatcccgctg ttttggctgg
     1261 tgagctttat cccgaaggca ttcccattct cgctgaagaa gacttagccg aactcatcga
     1321 aaaatacgaa gttgacgaag tctgtttcgc ttacagcgac gtgccctatg actacgtaat
     1381 gtccaaagct gctatcgtca atgctttagg tgctgatttc cgcatgcttg gcactcgcga
     1441 gacccagctt gagtccacca aacctgtcgt tgccgtttgt gctacccgca ctggctgtgg
     1501 caagagccaa accagccgcc gtgtggtcgc tgatctgcaa gaccttggtt acgaagtcgt
     1561 ggctatccgc catcctatgc cctatggtga tttggcaaag caagctgtgc aacgctttgg
     1621 cgagctggct gaccttgaaa aacacgaatg taccatcgaa gaacgcgaag aatacgaacc
     1681 ccatatcgaa aatggtgtgg tcatttattc tggcgttgac tacgaagcca tcctgcgcga
     1741 agcagaaaaa gaagccgaca tcattgtctg ggacggcgga aacaatgaca tgcctttcct
     1801 taaacccgac ctgttgatcg tcgttaccga cccacaccgc gctggtgatg aattagctta
     1861 ctatcctgcc gaagtcaacg tacgatctgc tgacgttttc gtcatcaaca aagtggatac
     1921 cgctgatcct gaactggtcg aatttgtccg ccgcaacttg ctggatacta atcccgaagc
     1981 cattatcgtt gatgccgctt cacccatttt cgtggaagat ccctttgcca tccgtggcaa
     2041 acgtgttttg gcaattgacg aaggtccaac aatcacccac ggtgatttgc cctacgactt
     2101 tggctacgtg gctgcccagc gctacggtgc cggcgaaatt gttgacccac gaccctttgc
     2161 cgttggcgat ttgaaggaaa cttttgaaaa cttcccccac ctggccaacg ttctgccttc
     2221 aatgggctac ggcgaaaaga tgattcgtga ccttgaagcc accatcgaag cctccgatgt
     2281 ggatatggtc gtgagcggta cgccaatcga cttgagccgc gtaatgaaaa tcaacaagcc
     2341 cttggttcgt gtgacctacg aattgcagga aattggtttc ccaaccctgt ttgatgtgct
     2401 tgaagaagaa tttggctgga aagacgaaga agacgaagag taaattttcg tctttgaaat
     2461 gaagataaaa agggtcgggt tctcccggcc cttttgttgt tgatgggtgt agacataaaa
     2521 ctctataaag catcggcgcg gactggtgtt caagtaaaac taaattgttt gctaacctat
     2581 cccgccgagc ttgccaaact gatgcctacg gcgagacgcc ggggcgagca acggaagaaa
     2641 aaacgttggg ctataatctc agggctgatt gtgttaaact tatcctatag aaagaaacta
     2701 aggaataact atgtttgttg attcagttgt aatttgggtt gcgtccggta agggcggtga
     2761 tggcattgtg cacatgcgac gtgaaaaata tcgcgcgaaa ggcggcccgg atgggggtga
     2821 tggcggtcgg ggcggtgaca tcattttcga agtgctgcca acaatgaaca cccttaatcg
     2881 tttccattac aacgagcgtt ttcaggctga ttcaggcaag cctggtggtc cgaataaccg
     2941 cagtggaaaa agtgcggctg atttgaccat tcatgtcccg cctggcactt taatctataa
     3001 cgacgaaacc accgaactaa tcggcgacct ggttacgccg ggtcagaaac tgattgtggc
     3061 taagggtggt cgcggcggac gcggtaatca gcatttcgcg accagtcgcc agcaagtacc
     3121 cctgacggct gaaaaaggcg agcccggtga agaattcacg ctaagacttg aattgaaact
     3181 tatcgcggac gttgggttgg ttggtatgcc aaatgccgga aaatcgtcga ttttggcgtc
     3241 cacaacccgt gccaaaccca agattgccaa ctatcctttc acgacactct ttccaaattt
     3301 aggtgtggtt gagcttgatt tagacaacac aatggtgctg gcagatatcc ccggtttgat
     3361 tgaaggcgcg tctgaagggg tgggtttggg ccatgatttt ttgcgccacg tacagcgcac
     3421 gcgcgtttta atccatgttt tggacggtat gtcggaagat ccgcttcagg attttgaagt
     3481 tatcaacagc gaactggaat ttttcgatga gcatcttagc cggtcaccgc aagttgtcgt
     3541 cttgaataaa atggacttgc ccgacgtgca ggaaaagttt gagtccatcg ccaaaaacct
     3601 gcaggaacag ggcattgaac tgatgccagt ttcagcagca actggcctga atatgaaaga
     3661 gctgatgtgg aaggcttatc aggtactgca agaaacgccc cttgaagaga cgcaagaaga
     3721 attgccggtt taccgtgctg atgtagaccc gcgcgctttt gaaattgagc aaatcgaaga
     3781 ggatacctgg ctggtgacgg gtaagatggt tgaaaaagca gccgcgatga cgtattttga
     3841 ccaggtcggt tcggtgcgtc gtttccaacg cctgatggaa cgcctgggtg ttgaaaaagc
     3901 cctgcgtaat gatggtattc aggaaggtga taccgttgtt gttggcgaat gggaactgga
     3961 gtggtgggac tagttcttta cggaaactac cgtttggaag ccacaagttg taaagattaa
     4021 gctgattgac ttaaaaatgt tcaccggaca ggtgaggggg gcacccgccc ggcgaacaaa
     4081 ataattatca tttaacggtt ttggaatgtc aagtaatttg acattaaaag ggcttgtttt
     4141 ggggaatact gatgaaaaat tacagacaac tgtataaaaa tatttagtaa caaaaaaggt
     4201 ctcatctaca ggctaaaatg gatgagacct tctgtgatta attgagcttt tttaacactc
     4261 actatgcccg caggtgtaac atttgcgaca accttcttcg ttgatgagcg tcgcttcgcc
     4321 acattcgggg cagagttcgc caacacgctg gaaggggttg cctgggctgc tgtggctttc
     4381 aggtacctgg gtctgtgtca tgggcaaagc ttcttgtggg ggcatgtata taggtcgtgg
     4441 ttcctgttca aagtgttcct ggcagagata ttcatccaaa gccttggaaa tgccatcagg
     4501 caaggaacgc acccggttgc tgccaaagcc cagtgagcgt ccaccgccga tgttgcccag
     4561 ttgttgcatg acgatgcgca aacgttcgcg gggttggatg ggcgaggcga tgcgtaagat
     4621 gtaggagata aggcgaccga tcgcttcgga gtgggcggcg gtttcggagc cggctttaga
     4681 agtgttgatg aagacttcaa agggttgtcc gccaccgttt tcgttgatgg ttacaaaggc
     4741 tttgcccagc ggggtgccta tcgagaaggt gtagccttgc aaggcacgtg ggcggggttt
     4801 tttgctgtct tgccagaagc tgagttgcgc gggcaagtct tcgccataaa gcaaggtttg
     4861 ctgaacggca ggcgttgcgg gagcagcagc gggggcgtct ttggcttcgg atgtggcttt
     4921 ggtttccaaa accaccacgt cgcgcgagcc ggtgatatag acggtaatac ccttgcaacc
     4981 cagttcccag gcttgcttga aggcatcggc aacttcttct tcggtcgtgc cggcggggaa
     5041 gttgatggtt ttgctcaggc tgttatccac aaagcgttgc agggcggcct gggtgtgaat
     5101 atgttcgtca gcggtgatat cagccgaaac aacgaagaca tcgcggatat gcgcgggaag
     5161 ttcttcgata tcctggcagt tgccattctc gagtactttt tcgatgatct ccgctttgct
     5221 ttcttcggaa aggtcagttt tatccaacgc gttttggaag tgtgggctaa cgtaggtaag
     5281 cgtgagtttt ttaccgcggt cgtccacatg gcgcatataa gccagggcga aaaccggttc
     5341 gcagccgtag ccttcaatgc ccgccacagt accgatggtg ccagtgggcg cgatggtggt
     5401 ttgggcggcg ttgcgaatgc cgtgttggcg gatgccctgg atgatgtcgt cccagttgat
     5461 ggctgggcgt ccccagtcgc tggtgtaggg cttgagcggg gtgggagcct gccatttgag
     5521 attttcgggg tcgtagatgc tgcccttgat agcagggaaa gcgccttttt gttcagccag
     5581 ttgaatgctg gtgagcattg aataataacg gatgtattcc ataatttgcc cacagaaatc
     5641 gagcgctgct tcagagccat agcgcacgcc agcgtggtac atcaggtcgg ccaggcccat
     5701 gatgcccagc ccgatgcggc gcgcgttcag cgctgattca cgcagttcgg gcacagccgg
     5761 tacgtaggcg tttttgtcaa ccacgttatc caggaatttg gtagcgagaa tggtgctttc
     5821 tttgagcttt tcccagtcga tgctgccatt ttcgccgcaa tgttcagcaa ggttgatgga
     5881 acccaaacaa cagttttcgt aggggccaag ccactgttcg ccacaggggt tggtggcttc
     5941 aagatgatag agatgtggca cggggttgtc gcggttggca gcgtcgatga agagaacacc
     6001 cggttcgccg ttgtggtggg cttgcctgac aagactgtca aaaagcgcgc gggcttttac
     6061 ggtcttgaac gttttaatgg gcagaccttg ttccctggcg tcttcaatgg tgccggagaa
     6121 ttttttatag gatgggtcgt gcacgtcggg gaagaccaga tcccagtccg cgtcgttttt
     6181 aacggcttcc atgaattcat cagtgattgc cagtgagata ttaaagtttg tgatggcggt
     6241 ttcgctggtt ttgcaggtga tgaacttttc cacgtcggga tggtcaacgc gcaaaatgcc
     6301 catattcgcg ccacgacgtg tgccaccctg ggcgatttcg ccaaaggcgt tgtcgtaaac
     6361 cttcaggaag cccacggggc cggtggcttc gcctgcactg gagtttaccg tggagccact
     6421 ggggcgcaat cgcgagaagt tgaagccatt gccaccgccg gtttgctgaa tgagcgcggc
     6481 attgcgcagg gtgctaaaga tgccatcgct ggccctgccc atatcatctg agataggcaa
     6541 aacaaaacag gcagccagtt gtcccagggg tgttcccgcg ccggtgaagg tgggcgagtt
     6601 tggaaagaac gcttttgaga ccagcagatg gtaaatctgg attgccaggg cgtgtttttc
     6661 ttcgtcgctg ctgtcaacgt cagcgatgtg ccaggcgacg cgccaaaaca tatcatcgat
     6721 ttcttcaacg ggttcaccgt ctaaaccgcg tcgcaggtag cgtttttgga gaattttgat
     6781 ggaattctcg ctgagttcaa tggaaggtaa accttccggt ttggggggag tcggcaacaa
     6841 gccgtgcttg ggtgtggctt cggtcatgat gatacctcta ataattattc ttcttcagat
     6901 aacagatgat ctatctcgtc acggacggtg tgcaggtcat ccatacgtaa gtaaacaatg
     6961 gcataacgaa tgtaggcgat gtggtccagt tctttaagcc cttttacagc aagatcgccg
     7021 ataacacgcg agttgatttc ggagcgaccc cgtttttgaa gctcggcttc aatttggtca
     7081 acgagttttt caatcatggt ggcagtaacc ggacgtttgg acgaggcttt gcgtaagcca
     7141 gcgagcagtt tttcgcggtc gaaagtttcg cgggtaccat cgcgtttgat gatgagtggg
     7201 gttgtaagaa tagggcgttc gatggtgcta aagcgctgtc cacagttgag acattcgcgt
     7261 cgtcggcgta taccaccctg ggcttctttg ttcgtatcaa cgacgctgct gtcgttatgc
     7321 tggcaatgtg gacacttcat aagaactcct ggtaatatcc ctatatgtta gggttgaaaa
     7381 ttaatagacc cctacatatg gcgaaattca ttctagcaca cgttaagaaa gcttgcaagt
     7441 aaaaaatgta aattaaagct taagaaatgg ctttctttaa cattgtctaa atcttgcgcg
     7501 tagcttagcc gtttctcggg ggtggatatt tattttgcat aaattctctt gttttttggg
     7561 tggtttaacg ggtggtaaag gcatgattaa gaaatcttgc aaacataata ataaataaaa
     7621 cttctccttg aaaacaaacg tgcaaaatcg tcctttctca tcacgggaaa ggacgatttg
     7681 ggaagc
//
LOCUS       DAOSFC010000029         7490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_8518_length_7490_cov_4.829052, whole genome shotgun sequence.
ACCESSION   DAOSFC010000029 DAOSFC010000000
VERSION     DAOSFC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8518_length_7490_cov_4.829052"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..1860
                     /locus_tag="PK791_01455"
     CDS             220..1860
                     /locus_tag="PK791_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HOH92011.1"
                     /translation="MQETTMTLNNLLACKSLRYARIIVLPKDLNVPVTGINIIEATDI
                     EKWAKKGMVLLTSYYALQDLDHAGLNEFFLKLQSIGITCLIVKINRLLKEVPQVFIEL
                     CKALDILLIEIPETTKYEEIMVEVLSFILSRREQRLQLYYHLSEISTRMAIEMLSMRE
                     ILSEFKKMLLFDLSIQNPAGELSVSTNPALANFECFEELPMEHSEYMTVEYKRFRYRC
                     SKNQNEPETSFVHVDLAPVGGDRYALIVHEEPDRRLDINDIIIVENLVRNVQLELLRE
                     YSGKQLKLLNKNALVGDILRGVIKSQDELNTIYQQLELDPNDIVRVMIIDYYPEVQSE
                     NLDLFNLRTKIRTSIQNKQTGTVYFIASSYDQFIIPQATTGQNMTAADFQTLVAQHIH
                     DYPEYMRLRFHGGISSFHQLKELPLADLQSKAISRFISNNYPNNYIEEYDNLGIFKLF
                     IGEDDTKLNEHIHPELKNLYENHPDLYETLYVYLSTGSSFARTAEELYLHPKTVKYRI
                     EKIKTLLSLDLENLHDLSILFTSIEILNFQAGISTLNA"
     gene            complement(1954..2610)
                     /locus_tag="PK791_01460"
     CDS             complement(1954..2610)
                     /locus_tag="PK791_01460"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF005302.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HOH92012.1"
                     /translation="MILEKEREKIVETGKRMIDARLTTGTGGNLSIFNRKENLIAIKP
                     SGVPYQSMRAQDVVVLRPDGERVDGVLKPSSETKFHLAIYHARPDVDAILHSHQVHAT
                     TIACMGIELPAVHYLLAFSGDKVPLAPYATFGTQALSDNILSVLGNYKACLLENHGLI
                     TLGASLDEAFDTAEALELVAQIYIQAKSIGEAKILNSEQMNVVIEKFKTYGKQDSETK
                     "
     gene            complement(2642..3592)
                     /locus_tag="PK791_01465"
     CDS             complement(2642..3592)
                     /locus_tag="PK791_01465"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOH92013.1"
                     /translation="MTQELDSKKNDNPQAKKDTPIAWLVRLLKGFISGVGAITPGLSG
                     GVMMVVFGIYEPLLKWLADIRQNFLRNLLFFIPVGIGGVVGVVAFSAAIDKAYELNAV
                     MFTWLIIGFIVGTYPSILRTAGKEGRKAWHIIMMLFIAGVLFYALRWMQSNLDISMPQ
                     NFWIWMMCGALTGLGLIVPGLSPSNFLMYMGLYQPMAAGVKSLNFGVIIPMLLGLVAV
                     IFALAKLISWLFKKHYALLYHIILGVVFGSTLAIVPIVSETKGVMIGADAFPLDKFPQ
                     LASINEPKSIVICVLLALAGFAASFLLAKLDEKHPHESLF"
     gene            3740..4495
                     /locus_tag="PK791_01470"
     CDS             3740..4495
                     /locus_tag="PK791_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOH92014.1"
                     /translation="MNRIENVGLEVFENVPSNDCDLTGRFTPANVFKKLTELAGINAI
                     QLGFGFDDMLAHDLYWVLSRMKVKFLRRPNAGELLQWRTWPKTIQQKLFYVRDFKILD
                     ENQQTVMLASSAWLVIHATKRRLLPPNRLPGLDLPSLEHLVALDESLEKLQIDDTSEA
                     FTMTARYSYIDMLGHMNNARYVEAITNAVPFDLMTKRELDWIQVNYDKEVRPQERLSI
                     RVAELSEDLYGVEGFNLDSEQQAFTAQLKFLPE"
     gene            4857..4940
                     /locus_tag="PK791_01475"
     tRNA            4857..4940
                     /locus_tag="PK791_01475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4891..4893,aa:Leu,seq:taa)
     gene            complement(5197..7137)
                     /locus_tag="PK791_01480"
     CDS             complement(5197..7137)
                     /locus_tag="PK791_01480"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOH92015.1"
                     /translation="MPFKNLFSTDMIDGYQLNLRDQKIPLLAGEVHFFRMDTSVWERS
                     VEAIKVLGLPMISTYLSWRRFEKIEGIFDFKGTTDPRLNLKAFLEICQEKGVYVILKP
                     GPWICAEETNGGYPDWLVERKSLQVLDADGNVVLGYNPPFNSPIPSLFHQDYWEALTR
                     WLKAVDDFVRPFIHPSGPIVLMQLDNEPCYTFHDRFLESDYNPVITGPHGVFKKWLEE
                     KYSSIDELKQVYGLETKSVSDLKAPRTVTESSPAALLRDWTLFKEWSLAEHVNRIGLL
                     HRKNGIEKIPFTINFNLHPQLSTPNNWALLEQASGLGGFDYYPELPMRWSFFTRMVMA
                     VTYSRLINKIPWSPEMMTGIWSFEGQPNQSHSFSVDDYRYLYFTCLAYGLKGMNFYML
                     ADRDNWIDSPINGMGEITEYANPVFDVVKLIRAHPNFLDYRPVVNLALLFNHDHAREA
                     YYLNETPSLLASSSGARAYKIFEDTFDFLLRKNITPALVDPEVHKISVIETPILIVVV
                     SGHLSSELLTYLAEGGTLICITLDGNNFLGADMRPNFHLCNANSLHEKLTEILLCLNI
                     NPTVPLNNPKIISVVQKYEEAHLLFLINSTDEEQAAEVQLKKNQVFQDFFDCERQLSK
                     SKPQIVLRPRSVRVYRSIETEK"
     gene            complement(7156..>7490)
                     /locus_tag="PK791_01485"
     CDS             complement(7156..>7490)
                     /locus_tag="PK791_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH92016.1"
                     /translation="LPKEIEEAAEMDGLTPFGVFWKITIPLAAPAIFTVSLFSFITGW
                     VDLLFGMSFSTVTEAMPLTVGLLQMQTGYKIYWGALMAGGIYLTLPTFVIALLFQKYL
                     LKGMRTGY"
BASE COUNT         2267 a         1683 c         1544 g         1996 t
ORIGIN      
        1 attgaatctt gataattgtt tttgcggatg atcgttttta gcatttttct ccttttcgaa
       61 ctaatttaaa ttgtttgaac ttgatacgac tgaaaacaag tacagaacgc accggttaca
      121 atcgtaaatt taagtttatc agggtattat tcatctgtca ttagcagttt tggggaaatt
      181 gtaaagtata atttatctat caaggataaa aggtgcgcca tgcaagaaac aacaatgaca
      241 ctaaacaatt tactggcttg taaaagctta agatatgccc gcattatcgt cctgccaaag
      301 gatttaaacg ttcccgttac ggggataaat attattgaag cgactgatat tgaaaaatgg
      361 gcaaaaaagg gcatggtcct tttaaccagc tattatgctt tgcaagacct tgaccacgct
      421 ggcctaaatg agtttttcct aaaacttcaa tctatcggca ttacttgttt aatcgtcaaa
      481 ataaaccgcc tgttaaaaga agttccacag gtcttcattg aactttgtaa agcacttgat
      541 attctcctga ttgaaatacc tgaaacgaca aagtatgaag aaattatggt tgaagtgctt
      601 agctttatcc tatccagacg cgaacaacgc cttcagcttt attaccacct gtcagaaatc
      661 agtaccagaa tggcaattga gatgttaagt atgcgcgaga ttctgtctga attcaaaaaa
      721 atgttgcttt ttgacctaag cattcaaaat ccagccggtg aacttagtgt gagcactaat
      781 cccgcactgg caaatttcga atgttttgaa gaactcccca tggagcattc cgaatacatg
      841 accgttgaat acaagcgttt tcgttacaga tgctctaaga accaaaatga gcctgaaaca
      901 agcttcgtac acgttgattt ggcaccagtc gggggagatc gttatgcgct cattgttcat
      961 gaggaacctg atcgccgtct ggacataaac gacattatca ttgttgaaaa tcttgtccgc
     1021 aatgtacaac ttgaactcct gcgcgaatac agcggtaaac aactcaagct tctgaacaaa
     1081 aatgccttgg taggagacat cttgagaggg gtcataaaat cacaagatga actgaacacc
     1141 atttaccagc aactcgaact tgacccaaac gatatcgtgc gggtgatgat tatcgattat
     1201 taccctgaag ttcaaagtga aaacctggac ctgtttaact tgcgcaccaa aatacgcacc
     1261 agcattcaaa acaagcaaac tggcacggtc tattttatcg cttcaagtta cgaccaattc
     1321 atcatacccc aggcaacgac tggccaaaac atgactgcgg ctgatttcca gactttggtg
     1381 gcacaacata tccacgacta tccggaatat atgcgtttgc gttttcatgg aggcatcagc
     1441 agttttcatc agctaaaaga acttcctttg gcggatttgc agtcaaaagc catttcccga
     1501 tttatctcca acaactatcc caacaattac attgaagaat atgataattt aggcatattc
     1561 aaactcttta taggtgaaga tgatacgaaa ctgaacgagc acattcaccc agagcttaag
     1621 aacctttatg agaatcaccc tgatctttat gaaaccttgt atgtttatct aagcactggt
     1681 tcaagcttcg cgcggactgc tgaagaactg tacctgcacc ccaaaacagt taaatatcgc
     1741 attgaaaaaa ttaaaacgct cctgagcctg gatttggaaa accttcacga tttatccatc
     1801 ttgtttactt cgatagagat tctgaacttc caggcaggta taagtacttt aaacgcctaa
     1861 aacaacccta accgctattc gtttgctatt tttagggaga tttatctatg cgcaatgcgt
     1921 ggagacggtg cgttttttac caaaagagcc agtttatttt gtttccgaat cttgcttgcc
     1981 gtaagttttg aacttttcaa taaccacgtt catttgttca gaatttaata tttttgcttc
     2041 gccgattgat ttggcttgaa tgtatatctg cgcgacaagt tccagggctt cagcggtatc
     2101 aaaagcttca tccaaactcg cacccaaagt aatcagcccg tgattctcaa gcaagcaagc
     2161 tttgtaattc cccaaaaccg acaaaatatt atccgataat gcctgggtgc caaacgtggc
     2221 atacggtgcc aaaggcacct tgtcaccact gaaagccagc aggtaatgca ccgcgggcaa
     2281 ctcaatgccc atgcacgcaa tcgtcgtggc gtgcacctga tggctgtgca aaatagcgtc
     2341 aacgtccggg cgcgcgtggt aaatcgccag gtggaatttg gtctcactgg atggtttcag
     2401 aacgccatca acgcgttccc catcagggcg caaaacaacc acatcctgcg cccgcatgct
     2461 ttgataaggc acgccactgg gcttgatggc aatcaagttt tccttgcggt tgaaaatgct
     2521 cagatttccg cctgtgcctg tggtcaggcg cgcgtcaatc atacgcttgc cagtctcaac
     2581 gattttttct ctttctttct ctaaaatcat agcgcgcctg aatcctgtta tctaaaaaag
     2641 cctaaaaaag cgactcgtgt gggtgcttct cgtcaagttt ggcaagcaaa aacgatgccg
     2701 cgaagcccgc cagtgccaaa agcacgcaga tgacaattga ttttggctcg ttgatagagg
     2761 ctaattgcgg gaatttatcc aggggaaaag catccgcgcc aatcatcaca cctttcgtct
     2821 cactgacgat gggcacgatc gccaacgtcg agccaaagac cacacccagg ataatgtgat
     2881 aaagcaaagc gtaatgcttt ttgaaaagcc agctaatcag cttggctaac gcgaagatga
     2941 ccgcgaccag accaagcaac atcgggataa tcacaccgaa attgagcgat tttacacccg
     3001 cggccatcgg ttgatacaag cccatataca tcaggaagtt actcggactc aaacccggca
     3061 caatcagacc cagcccggtc aacgcgccac acatcatcca aatccaaaag ttttggggca
     3121 tgctgatgtc caaattgctt tgcatccagc gcaaggcata aaaaagcaca cccgcgatga
     3181 acagcatcat gatgatatgc cacgctttgc gaccttcttt gccagcagtg cgcaaaatgg
     3241 acgggtaagt gcccacaata aagccaataa tcagccaggt gaacatcacc gcgttgagtt
     3301 cgtaagcctt gtcgatcgcc gcggaaaaag cgaccacgcc aaccacccca ccaataccaa
     3361 ccgggataaa gaaaagcaga ttgcgtaaga agttttggcg gatatcagcc agccacttga
     3421 gcaggggttc gtaaatgcca aagaccacca tcatcacccc gccagaaaga ccaggggtaa
     3481 tcgcgccaac gccggagata aagcctttca acaggcgaac aagccaggcg ataggcgtat
     3541 cttttttggc ttgcggattg tcgtttttct tgctatcgag ttcttgggtc atgggtccat
     3601 cctaatcatt tttatatggt ttcattctac aacaaaatta aaatatatgt ttcattgttt
     3661 aaatgcgtaa ctaaaacgta ttttgatgtg tttctaagat taagccaata gactggtgat
     3721 aagtgaaaga aggttagtta tgaatcgcat agaaaatgtt ggtttagaag tttttgaaaa
     3781 tgtcccaagc aacgattgcg acctgaccgg tcgctttaca cccgctaatg tctttaaaaa
     3841 gctgactgaa ttagccggca tcaatgccat tcaactcggt tttggctttg atgacatgct
     3901 tgcccatgac ctttactggg tgctctcgcg catgaaggtg aaattcctac gccggccaaa
     3961 tgccggagaa ctactccagt ggcgaacctg gccaaaaaca atccaacaaa aactctttta
     4021 tgtccgtgat tttaaaatcc tggacgaaaa ccagcaaacc gttatgcttg ccagttcagc
     4081 ctggctggtg attcacgcca ccaaacgccg attactgcca cccaaccgtt tgcctgggct
     4141 ggacttgcca tcccttgagc atcttgtagc ccttgatgaa agcctggaaa aattacaaat
     4201 tgacgataca tccgaagcct ttaccatgac tgcgcgctac tcgtatatcg atatgctggg
     4261 tcatatgaac aacgcccgtt atgttgaagc gattaccaat gccgtgccct ttgacttgat
     4321 gacaaagcgc gaactggact ggattcaggt caattacgac aaggaagtgc gcccacagga
     4381 gcgcttgtca atccgcgttg ctgagttgtc tgaagatttg tacggtgtgg aaggtttcaa
     4441 tcttgattcc gaacaacagg cattcaccgc ccaactaaag tttttgccgg aataaaaaca
     4501 ctttctcccg caaaaagacc gctttaagac caggcaaacc aaataatcca cccaaaggct
     4561 tagctgaatt agtcctttat gccgatgtct gaaaacgcgt aaaaatctat actgggggtg
     4621 taaaaggaga taagacatga taccgacatt aatcatcacc accatcatcg ctgttttggg
     4681 caccctgagc cccctgctgg tttcagacga catgcacgaa atcgtggaac tatagcttaa
     4741 taattatcaa ccacaaacgt tttcttgatt cttcaagggt cactttgcaa aaaaaagcgg
     4801 gaaaacacaa aatttcccct tgcataaatt aaaacaatgg taaaatgatt taccttgggg
     4861 ctgtggcgga ataggcagac gcaaaggact taaaatcctt cgatggtgac atcgtgtggg
     4921 ttcgaccccc accggcccta ctcaaaaacg aagatgcgaa ctctatttgc aaaacatgca
     4981 tagtgtcgct attcatagat tttaagacag tcaattggct gtctttttgt taaggtttct
     5041 gctcattgac cccacaatgt gggcggaata gaaaatgctc attatgcgag agtaaagcaa
     5101 cgatctattc agagaaaaaa agcataagag ctttaataga gattcaaatc ggtatcatgg
     5161 gtactaagca acaaatacac gattagagac cctgttctat ttttcagttt ctatggatcg
     5221 atacacacga acactgcgag ggcgcaagac aatttgcggt ttagatttgg acaattgcct
     5281 ctcacaatca aaaaaatcct ggaaaacctg gttttttttc aattgaacct cagcagcttg
     5341 ctcctcatct gttgagttaa ttaagaaaag cagatgggcc tcctcatact tttgaactac
     5401 agagatgatt ttcgggttat tcagtggaac ggttggatta atattgagac atagaagaat
     5461 ttcggttagt ttctcgtgca aactgtttgc attgcaaaga tgaaaatttg gcctcatatc
     5521 tgcgccgaga aagttgttac cgtctagggt gatacaaatc aatgttccac cttcagccag
     5581 atatgtcaag agctcactgg aaagatgtcc tgaaacaacc acaataagaa taggcgtttc
     5641 gatgacagaa atcttgtgaa cttcaggatc aacgagagct ggggtaatat tttttctcag
     5701 gagaaaatca aaagtatcct caaatatctt atatgcccgg gcacctgatg aacttgccaa
     5761 caaactaggt gtttcattca gatagtaagc ttcacgagca tggtcatgat taaacaataa
     5821 agccaggttt actactggac gataatccaa aaagtttgga tgggcgcgga taagtttaac
     5881 cacgtcaaaa actggattgg catattctgt tatttccccc atgccattga tcggagaatc
     5941 gatccagtta tcgcgatctg ccaacatgta aaagttcata cctttcaacc catatgccag
     6001 acaggtaaag taaagatagc gataatcgtc gacagaaaaa ctatgcgact ggtttggctg
     6061 gccttcaaaa ctccaaattc cagtcatcat ttcaggcgac cagggaatct tattgatcag
     6121 gcgagaatag gtcaccgcca taaccattct tgtaaaaaaa gaccaacgca ttggaagctc
     6181 agggtagtag tcaaaccctc ccagtccaga agcttgctca agcaatgccc agttattagg
     6241 agtagaaagt tgagggtgta gattaaaatt aatcgtaaat ggtatctttt cgatcccatt
     6301 tttacgatga agaagtccaa tacgattgac gtgctctgct aaagaccact ctttaaacaa
     6361 agtccaatcc ctcaataacg cagcaggact tgactctgta acggttctgg gagccttcag
     6421 atctgaaact gatttcgtct ctaatccata aacttgttta agttcgtcta tgcttgaata
     6481 tttttcttca agccattttt taaaaacacc atgtgggcca gtgatcacag gattatagtc
     6541 cgactctaag aaacgatcat ggaaagtgta gcaaggctca ttgtcaagtt gcattagaac
     6601 aatcgggcct gatgggtgta taaatggacg aacgaaatca tctacagctt ttagccaacg
     6661 agttaatgct tcccaataat cctgatggaa taatgaagga ataggagagt taaaaggtgg
     6721 gttgtatcct aaaacgacat taccgtctgc atcaagaacc tgaagcgatt tgcgttccac
     6781 tagccaatca gggtatccac cgttagtttc ttctgcacaa atccatgggc ctggttttaa
     6841 aataacgtaa acaccttttt cctggcaaat ttcaagaaaa gctttgaggt tcaagcgagg
     6901 gtctgttgtc cctttaaaat cgaagattcc ttcgattttt tcaaaacgtc tccaactgag
     6961 ataggttgat atcataggca gtccaagcac ttttatcgct tcaacgcttc tttcccagac
     7021 ggatgtatcc atacggaaaa agtgaacttc tccagccaag agtggaatct tttggtctcg
     7081 taaatttaat tgatatccat caatcatatc agttgaaaaa aggttcttga aaggcatttt
     7141 tagtctcctt gacagttagt atccagtgcg catacccttt agaaggtatt tctggaatag
     7201 aagggcaata acgaatgttg gtaaggtcaa gtatattcca ccagccatca atgcacccca
     7261 gtaaatctta taaccagttt gcatttgtaa taatccaacg gtaagtggca tggcttctgt
     7321 aactgtagaa aaactcattc caaatagcaa gtcaacccag ccagtgatga aactgaagag
     7381 cgaaacggta aaaatagccg gcgccgcaag gggaatcgta attttccaga acacgccaaa
     7441 aggtgttaac ccatccattt cagcggcttc ttctatttcc ttgggcaggg
//
LOCUS       DAOSFC010000030         7467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_8547_length_7467_cov_4.835942, whole genome shotgun sequence.
ACCESSION   DAOSFC010000030 DAOSFC010000000
VERSION     DAOSFC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8547_length_7467_cov_4.835942"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..295
                     /locus_tag="PK791_01490"
     CDS             50..295
                     /locus_tag="PK791_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH92017.1"
                     /translation="MTTEKTFELVKEVVIDVLKIDGDTITMETRFIEDLKADSMDQFF
                     LIDGFCEKFDIDIDDEAARAIQTVGDAVEAIDKAIAK"
     gene            complement(349..1218)
                     /locus_tag="PK791_01495"
     CDS             complement(349..1218)
                     /locus_tag="PK791_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOH92018.1"
                     /translation="MSILIDQNSRIIIQGINGKAGSFFAKRMHDYGNNVVGGIAPSHG
                     GEWTLNGKIPLFDTVHECVQSTDADTTVIFVPAYSATDACFEALDSGIKNIICISAHV
                     PMKDIAFIKSSLQNKDAILIGPNALGVFTPGKALAGVFPVDFAIEGNLGVISRAGSLC
                     YDVLMELYKNDIGISSVIGLGEDCLSGTSFVDCLDLFEMDAHTDSILIIGQPGYIQES
                     LAATHILESITKPVYAYVAGVGINTNKIIGHTEICIFDSQHNAQRKIEDLSNAGVRIA
                     RNLKDISQLINGR"
     gene            complement(1220..2356)
                     /locus_tag="PK791_01500"
     CDS             complement(1220..2356)
                     /locus_tag="PK791_01500"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOH92019.1"
                     /translation="MKLHEFQTKAIFKREGIPLPASFLSSSPVQAKQAASEIGYPVIL
                     KAQSLISGRGKAGGIRLTRNDAETESAAGDIFNIKLKGISIQQILVEKAIKVENELFI
                     GIRYDTTSGDPSFVLSVEGGISLEDNAPLSSSNSFIQTINPLLGLQAFQVRQACANLK
                     IAPFKWKIIEQTSLKLWHVFNNYDATLAEINSMVFNEEGHAVVIDCKLVIDDNALFRQ
                     REFAGFLDPAITSFAQAEARKFGHKLVRLDGNIAVISNSQGLASMTLDEFAQHGLNPA
                     LVLILDHNATVSSISAACQLVQEDPSVETVFLNIVSNMDDCCSIAQGINRTHQNTLSP
                     IPFVVRLKGSHCVEANKILTGQENLSLFETLDDAVQKLKEVLEE"
     gene            complement(2438..3508)
                     /locus_tag="PK791_01505"
     CDS             complement(2438..3508)
                     /locus_tag="PK791_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOH92020.1"
                     /translation="MARVFDVVEYPREMVDEIVKRFPETGVADLRIGSQVIVRESQAA
                     VFFRDGRALDVLGPGRHTITTANIPILTGLIGKLFSDRTPFTAEVYFVSMREFADRKW
                     GTPQPIIVRNTGVGLGVALLQGFGSYSFQVADPQQFVTQVVGQLHAYRTQDIENRLRT
                     MLLSKLQDVLGETTAKKNVLDLIGLTDELSAAVRAKAQDDFLALGLNLKTFYIGNLKP
                     SDKSAAELRDMGMLDMQTYMQLQAADAMRDAAQNPSGGAGLTAGIGAGMGVGQILAGS
                     LAGFQGQTPSQQPTQGQAPAQGQQGTSLPEMLTPENVAQYLNVSVDDVISTIESGTLK
                     AVKIGSAYRISRSALDEFLRSF"
     gene            complement(3526..4050)
                     /locus_tag="PK791_01510"
     CDS             complement(3526..4050)
                     /locus_tag="PK791_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92021.1"
                     /translation="MTDILNKLNEYKGGLEKLIAKIPGFKGYIALNDRRKSDKLLRET
                     IAREYEVQYQRISGVQRQLISEGELGAVGELENAAIKLRQFIDRVRTASYGYAGLFDA
                     IRIREAELETVYQYDAMLLDKNTEIASAIDNVEASIGTDGQAAAIRNLINHAQEAIDA
                     FNKRSDIMKGIVIQ"
     gene            4337..5101
                     /locus_tag="PK791_01515"
     CDS             4337..5101
                     /locus_tag="PK791_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOH92022.1"
                     /translation="MDEIRAQKIINDIHNGFWQVMDWVFPPTCPGCGIEGELICEECE
                     SKIVRLVGRQCKYCGLSVTGSDICGRCRDENHSYEAYRGYGYYTGVLRQAILRLKYQN
                     DIGIARILASYLETLVVSNDWNFDIVVPVPLSAKKLEERGYNQASRLAKPLAALLGKV
                     YKPDALVRLREVSSQVGLNKAARLENVKDAFRGNPKIAKGKSILLVDDVFTTGATLES
                     AASELMMVGARKVYALTLAKSYKIKMNIEQSPNIVV"
     gene            5127..5690
                     /gene="raiA"
                     /locus_tag="PK791_01520"
     CDS             5127..5690
                     /gene="raiA"
                     /locus_tag="PK791_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOH92023.1"
                     /translation="MALKIDLYTKELELTDRIHDYANKKIGKLDKFLSEIDECRVDLS
                     HAKTARNANERFIAQVTIRGKGFILRSEERADNIFAAIDATVEKMTRQIERFKGKHWR
                     RRADGAAKSSLEMEAEVEVDERPEPVIVRRKSFVLTPMDELEAMEQLMLLGHEDFFVF
                     YNANTNKINVLYRRRDGTYGLIDPIIG"
     gene            5760..6338
                     /gene="folE"
                     /locus_tag="PK791_01525"
     CDS             5760..6338
                     /gene="folE"
                     /locus_tag="PK791_01525"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOH92024.1"
                     /translation="MTDKKIHENKHHEIIEATKVLIKSIGEDLQREGLIRTPERVARA
                     SEEIFSGYTTDPDALINNALFNVDYDQMVVVKDINFYSMCEHHILPFFGKAHVAYLPK
                     GKVIGLSKIPRIVDMFARRLQVQERMTQQIATFIQETIHPLGVAVVIEASHLCMMMRG
                     VRKEDATMTTSAMLGGFRTHLDTRLEFLNRIS"
     gene            complement(6360..6722)
                     /gene="folB"
                     /locus_tag="PK791_01530"
     CDS             complement(6360..6722)
                     /gene="folB"
                     /locus_tag="PK791_01530"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOH92025.1"
                     /translation="MDIIRITDLEAIGYIGVKDDERANPQNLLISFHGVYDLSKAMQT
                     DNVSDTINYSTIAKRIKAIVAETKYRLLEALANHIMRELFYEFSFSEITLRIEKPHRV
                     ANTRLVGIEITRKAENYS"
     gene            complement(6733..>7467)
                     /locus_tag="PK791_01535"
     CDS             complement(6733..>7467)
                     /locus_tag="PK791_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH92026.1"
                     /translation="SMEIKGKNALVTGGAIRIGKAISSELAKHGVNIALHYHSSIDSA
                     IALKPELESEGIRVGLFQQDLSETEALPAFFSHIIETFGQIDILINNAGIYPRDRLKN
                     LKIDTLEALFGINLYAPLVLMREFAKQIPEDGHGKIINIIDAKIFKHDPETFMYRLSK
                     VALLEATKLAAMELAPNITVNAISPGLMMSLAGYEHLDMQAVAERRVPLKILGSGEIV
                     AQNLIHILKQDFMTGNNIILDGGEFI"
BASE COUNT         2279 a         1443 c         1582 g         2163 t
ORIGIN      
        1 cttaaaacaa gataatatga aaagaaacac tgtaaggagc ttgtaaagga tgacaactga
       61 gaaaactttt gaattggtaa aagaagtcgt aatcgacgtt ttgaaaatcg atggggacac
      121 aattacaatg gaaacccgtt tcattgaaga cttaaaagct gactcaatgg atcaattctt
      181 tttgattgat ggtttctgtg aaaagtttga tatagacatc gacgatgaag cagcgcgcgc
      241 aatccaaact gtaggagatg ccgtagaagc aattgacaaa gcaattgcca aataacaata
      301 atttttacat cataaacagt atcagctggc gaatagccag ctgtgcattt aacgaccatt
      361 aattagctga gaaatatctt tcaaattacg agctattcgc acacctgcat ttgacaaatc
      421 ttcaattttt ctttgagcgt tatgctgaga gtcgaaaata caaatttcag tatgaccaat
      481 aatcttattt gtgttaattc ctacacctgc gacgtaggcg tatactggct tcgttattga
      541 ttcaaggata tgcgtggcag ccaaactctc ctgaatatag ccaggctgac cgataatgag
      601 aatgctatcg gtgtgcgcat ccatttcaaa caagtcaagg caatctacaa aacttgttcc
      661 tgaaaggcaa tcttcaccta atccgataac agaactaata ccgatatcgt ttttatagag
      721 ctccattaaa acatcatagc atagcgaacc tgcacgagat ataacgccta gattcccttc
      781 aatagcaaaa tcaacgggaa aaacacctgc caaggccttt ccaggggtga aaacacctaa
      841 agcgttagga ccaatcaaaa tggcatcttt attttgcaaa ctgcttttta taaaggcgat
      901 atctttcatg ggaacatgag cagagataca aatgatgttt ttaatgcctg aatctaaagc
      961 ttcaaaacaa gcatctgtgg ctgaatatgc gggaacaaaa atgacggtcg tatcggcatc
     1021 ggtactttgc acgcattcat gtacggtatc aaacaaggga attttgccat tgagcgtcca
     1081 ttcgccaccg tgtgatgggg caatgccacc gacaacgtta ttgccataat cgtgcatgcg
     1141 tttagcgaag aatgaaccag ctttaccgtt aatcccttgt atgatgattc gtgagttttg
     1201 gtcgatgaga atggacattt tattcctcaa gaacctcttt taacttttgg acagcgtcat
     1261 ctaaggtttc aaataaacta agattctcct ggcctgttaa tattttattc gcttcaacac
     1321 aatgcgaacc ttttaaacgc accacaaaag gaatgggaga caatgtgttt tgatgagttc
     1381 tgtttattcc ctgggcaata gaacaacagt catccatatt gctaacaatg ttaagaaaaa
     1441 cagtttcaac agatggatct tcttgaacca gttggcaagc agctgaaata ctggaaaccg
     1501 tggcattgtg atcaagaatt agaactagtg cagggtttaa tccatgttgt gcaaattcat
     1561 ccagcgtcat gctggcaagg ccctggctgt ttgagatgac agcaatattt ccatcaaggc
     1621 gaactaattt atgaccaaat ttgcgggctt cagcctgagc gaagcttgtg atagcagggt
     1681 ctaagaaacc ggcgaattcc cgttgacgaa acagggcgtt atcatcaatc acaagcttac
     1741 aatcgatgac aacagcatgt ccttcttcat tgaaaaccat agaattaatt tctgcaagag
     1801 ttgcatcgta attattaaaa acatgccata atttgagaga tgtttgttca atgattttcc
     1861 atttaaaagg agctatttta agattggcac atgcttgtcg gacttgaaat gcttgcaaac
     1921 cgagtaaagg gttaattgtc tgtataaaac tgtttgatga agataatggt gcattgtcct
     1981 caaggctgat accgccttca acggatagaa cgaagcttgg atcacctgaa gtagtatcgt
     2041 agcgtattcc gataaaaagt tcattttcga ctttgattgc tttttcaacc aaaatctgtt
     2101 gaatggaaat tcctttgagt ttgatgttga aaatatcacc agctgcactt tcagtttcag
     2161 catcattacg agtcagcctt atgccgccag ctttgccacg accgctaatt aaagattgcg
     2221 cttttaaaat tacaggatag ccaatttctg acgcagcttg tttggcttga acagggctgg
     2281 aacttaaaaa ggacgctggc aggggaatac cttcgcgctt aaaaatggct tttgtttgaa
     2341 actcgtgtaa tttcattgtt gcaaaaacta aagctaaaga tagtaaaaat ataacacaaa
     2401 gtcctgattt tattcaggac tttgtgatta aggtaaatta gaaactgcgt aggaattcgt
     2461 caagtgcaga tctggaaata cgataagcac taccgatttt aactgccttt aaagtgccgg
     2521 attcaatggt tgaaatgaca tcatcaactg aaacattcag atattgagca acattttctg
     2581 gggtgagcat ctcgggcaag gaagtacctt gttgtccctg agcaggtgcc tgtccctgag
     2641 tgggttgttg gcttggtgtt tgtccctgga agccagcaag tgaaccagct aaaatttgac
     2701 ctacgcccat accggcacct atgccggcag ttaagccagc gccaccggac gggttttgag
     2761 cggcatcgcg catagcatcg gcagcttgca gttgcatata ggtttgcata tccagcatac
     2821 ccatatcgcg caattcagca gcagatttat ccgacggttt aaggttgcca atgtagaagg
     2881 ttttaagatt tagacccaaa gccaagaagt catcttgagc ttttgccctt actgcagcac
     2941 taagttcatc ggtcaaacca atgagatcaa ggacattttt cttggcagtt gtttcgccaa
     3001 gcacatcctg gagttttgaa agcaacatgg tccgtagacg attttcaata tcctgtgtgc
     3061 gatatgcgtg cagctgacca acgacctggg taacgaattg ctgtggatcg gcgacctgga
     3121 agctatagct gccaaagccc tgtaaaagcg cgacgccaag tccaacacct gtattgcgaa
     3181 cgatgatggg ttggggggtg ccccattttc ggtctgcgaa ttcacgcatt gaaacaaaat
     3241 atacttccgc agtgaatggc gttctgtcac taaagagttt gccaatcaaa cctgtgagaa
     3301 tggggatgtt cgcagtggta atagtgtggc gaccaggacc aagcacatcc agcgcacgac
     3361 catcacggaa gaagactgcc gcctgggatt cgcgaacaat tacttgtgaa ccaattcgta
     3421 aatcagcaac acctgtttca ggaaatcgtt taacgatttc atccaccatc tcgcgtggat
     3481 attcaacaac atcaaaaact cttgccatca aagacctcct aagatttatt gaataacgat
     3541 gcccttcatg atatcggagc gtttgttaaa ggcgtcgata gcttcctggg catgattgat
     3601 tagatttcta attgctgcag cttgaccatc agttccgatg gaagcttcaa cattgtcaat
     3661 agcactggcg atttcggtat tcttgtccag taacatggca tcgtattgat atactgtttc
     3721 gagttctgct tctctgatgc ggatggcatc gaaaagacca gcatagccgt atgatgcagt
     3781 acggacccgg tcgataaatt gacgaagttt aatagctgca ttttccagtt cgccaacagc
     3841 gcctaattca ccttctgaga tgagttggcg ttgcacacca gaaatgcgtt gatattggac
     3901 ttcgtattct cttgcaatcg tttcgcgcaa gagtttgtcg gactttcgac ggtcgttaag
     3961 ggcaatgtaa cctttgaaac caggaatctt agcgataagt ttttccagtc cacctttgta
     4021 ctcatttagt ttgttgagaa tatcagtcat tgttctcctt cttaatagaa atagcgttta
     4081 cttgattata tgataatctt ttgaaatagg gatatactaa agattatcta ctgtctttta
     4141 tcgactgatt tttattataa ttgattaatg caatttttga atggagtttt ttatgcgtcg
     4201 tggcacaaat aatccgtggg ggccattaga atatattctt ctaattattt tgtttatgtt
     4261 ggtgcttata accatatttt ctttgttttg gccagcaata aaattgtttt atgaacagat
     4321 aacccaaaag taaactatgg acgaaattcg ggcacaaaaa ataattaatg acattcataa
     4381 cggattttgg caagtgatgg attgggtttt tccacctacc tgtcccggct gtggtattga
     4441 aggcgaactt atctgtgaag aatgtgaaag taaaattgtt cgcttggtag gacgtcaatg
     4501 taaatattgt gggctgtctg ttaccggttc tgatatatgt ggaagatgcc gagacgaaaa
     4561 ccattcatac gaagcatata ggggttatgg atattacact ggcgtactca ggcaagctat
     4621 tttacgtcta aaatatcaaa atgatatagg catcgcccga atactggctt catatctaga
     4681 aacattggtg gtttcaaacg actggaattt tgatatcgtc gttcctgtcc cgcttagtgc
     4741 aaaaaagtta gaagaacggg gctataatca ggctagtcgc ttggcaaagc cattagctgc
     4801 tttgcttggg aaagtctata aacctgatgc actggtacga ttgagagagg taagttcgca
     4861 agttggcttg aataaagctg cccgacttga gaatgtcaag gatgcttttc gtggaaaccc
     4921 aaagattgct aaggggaaat ctattctgtt ggtagatgat gtctttacca caggcgctac
     4981 tttagaatcc gctgcatcag aattaatgat ggttggtgcc agaaaggtct acgctctgac
     5041 gctggcgaaa tcttacaaaa tcaaaatgaa tattgaacaa tcaccaaaca ttgttgtata
     5101 atacagaaaa atatacggag gttcaaatgg cactgaaaat agatttatat accaaagaat
     5161 tagaactgac cgatcgaatc catgactacg caaacaaaaa aataggcaaa cttgacaagt
     5221 tccttagcga aatcgatgaa tgtcgcgttg atttgagtca tgctaaaact gctcgtaacg
     5281 caaatgaacg atttattgct caggtaacaa ttcgcggaaa aggttttatt ttgcgatcgg
     5341 aagaacgcgc tgacaatatt tttgcggcca tagatgcaac ggttgaaaaa atgacccgac
     5401 aaattgaaag gttcaagggg aaacattggc gaagacgtgc cgatggcgct gcaaaatctt
     5461 cattggaaat ggaagcagaa gttgaagtcg atgaaagacc agaacctgtc atcgtaagac
     5521 gtaaaagctt tgtacttacc ccaatggatg aattggaagc gatggagcaa ctgatgttgt
     5581 tggggcatga agactttttt gttttctata atgccaatac caacaaaatc aatgtcctgt
     5641 atcgtcgtcg ggatggaacg tacggtttga ttgacccaat aatcgggtaa attaatgttt
     5701 tgccagcgag caaccctttc aatgggttgc tcgattcttt tcaataacag ataaataaga
     5761 tgacagataa aaagatacat gaaaataagc accatgaaat catcgaagca acaaaagttt
     5821 taatcaagtc gattggtgaa gatttgcaac gtgaaggctt aataagaacg ccagaacgcg
     5881 ttgccagagc cagcgaagaa atttttagcg gatatacaac cgatcccgat gctttaataa
     5941 acaatgcctt gttcaatgtt gactatgacc agatggttgt tgtaaaagat atcaattttt
     6001 atagtatgtg tgaacatcac atattacctt tttttggcaa agctcatgtt gcttatctgc
     6061 caaaaggaaa agtcatcgga ctttcaaaga taccccgaat tgtcgatatg tttgcgcgta
     6121 ggttgcaagt gcaagaacga atgacgcaac aaatagccac attcattcaa gaaacgatac
     6181 atcctttagg cgtggccgtt gttatagaag ccagccactt atgtatgatg atgcgtggcg
     6241 ttagaaaaga agatgcaaca atgacaactt cggcgatgct aggtggtttc agaacgcatt
     6301 tggatacacg gcttgagttt ctgaaccgaa tttcgtaatt aataaaacca taaaaagctt
     6361 tagctgtaat tttcagcttt tcttgtaatt tctatgccaa ccaatcgtgt atttgctaca
     6421 cggtgaggtt tttctattcg aagtgtgatt tcggaaaaag aaaactcata gaataattca
     6481 cgcataatat gatttgccaa agcttcaagc aggcgatatt tagtttccgc aacaatagct
     6541 ttaattcttt tggctattgt agaataatta atggtgtcag aaacattatc tgtttgcatt
     6601 gcttttgaaa gatcatacac accgtgaaaa ctgattaaaa gattctgcgg attagcgcgc
     6661 tcatcgtctt taacgccaat atagccgatt gcttcaagat ccgttattcg tataatatcc
     6721 ataaatatct ccttagataa attccccgcc atcgagaatg atgttattgc cagtcataaa
     6781 atcttgtttc agaatatgaa taagattttg agcaacaatt tcaccactgc ctagtatttt
     6841 tagaggtaca cgtcgttctg caacagcttg catatccaaa tgttcatatc cagcaagtga
     6901 catcatcaaa cccgggctga ttgcattgac cgtaatgttt ggtgctaatt ccatggcagc
     6961 cagcttggtg gcttccaata aagcgacctt gctaagacgg tacataaatg tttcagggtc
     7021 atgtttaaaa atctttgcat caatgatatt aataattttg ccatgtccat cttccgggat
     7081 ttgtttggca aattctcgca tgagaaccaa tggggcgtaa agatttattc cgaaaagagc
     7141 ttccagggta tcaatcttta ggttcttaag cctgtctctt gggtatatac cagcattgtt
     7201 gatgagaata tctatttggc caaaagtttc tataatatga gaaaaaaaag cgggtaaagc
     7261 ctctgtttcg ctaaggtctt gctgaaagag cccgacacga ataccttcag attctaattc
     7321 aggttttaag gctatggcag aatctataga agaatgatag tgcagggcaa tgttgacacc
     7381 atgtttagcc agttcacttg aaatcgcttt tccaattcga atagcgccac cagtaacaag
     7441 cgcatttttc cctttaattt ccataga
//
LOCUS       DAOSFC010000031         7457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_8559_length_7457_cov_4.479735, whole genome shotgun sequence.
ACCESSION   DAOSFC010000031 DAOSFC010000000
VERSION     DAOSFC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8559_length_7457_cov_4.479735"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PK791_01540"
     CDS             complement(<1..287)
                     /locus_tag="PK791_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92027.1"
                     /translation="MKKFQLKDLEIDWFLLINGFGSLLAGTVFANNRPGEWDLISLFS
                     TIIFWATLYLLQRTARRLSTPLVIDDTRLATGKRRLTVMDYLPGFGLLAVI"
     gene            443..2503
                     /locus_tag="PK791_01545"
     CDS             443..2503
                     /locus_tag="PK791_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOH92028.1"
                     /translation="MQHPLLSQTELFVVNTSEECEPYETTLCFFNHESLDELDGEGTG
                     LRAAIKHAKENLLSGIKINIVADYKIKTFPVLIDFPVVINGFDNAKITTQEPNCDHAM
                     LNIIESVTIQNLIIDDGACYDVSRDLIVVNSPNSVTIQKNTLENGKNAILFKDNSGNL
                     SVFFNDIRDNQLSAIRREAGTSTGKLKLTGNMITNNNPGGNQVFCHHSTLGNVNHNYW
                     GEDVFPSSASENCTFDDNKRLGAEPRQLSAGIAGEYINLERTYSDPIFGGFSAKSDQP
                     GNALFVIDHNNNQPFIDKMPRNYELTHCGKFYDVFMDENSTASLVSLRFSYDNSPCAE
                     VINSQQFCASNNENQYPLMWFDPKYGSTVTHGYDNVGAEPEGTGRYFGQTVRCNTANR
                     TIETDINNAFTQRPNLIDDLNYTPFTIGFDKTVVEDFTSSAAGSTTITLTWSSFSEAN
                     MTHYRLLKSDKSNGEFKPIGNDFSRKGDAQSGENYKYIDTGLSPETTYYYKLVVFNGD
                     TIQQTFGPVEAKTNALEPTNTHTPTRTSTQGRPTSTPYPISNSPTPTGFVYRSPTSTS
                     TKSYVKTSTPLPELTQNSQHQTMTAFFTTQTARYSDRTPAVDETPSYEDTSTEIIILN
                     TSTEESQNINVTFTPSPTQRGVNTDKSSRYHEEQSTPKWVFWLLLIVFGGYGIYLGTR
                     KNIH"
     gene            2597..3427
                     /locus_tag="PK791_01550"
     CDS             2597..3427
                     /locus_tag="PK791_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH92029.1"
                     /translation="MQKERVSDNVYWFQSEIYAQVTAGAIIGPQWAVLIDTLMPEETP
                     HIKHFLESELMMPVRYVINTHHHADHSWGNCFFPNATIISHDLCRKYMIEKSMSALNE
                     AVEGGMYFKDICIIPPDITVADGSLFLRIGKKQIQIFATPGHSDDSVAVLLEEDRILF
                     AGDSFMPVPFFVGGDYDVLLSTLIRYSEMGLENIIQGHGDIILRGEIDDKVQMNVDYL
                     HTLKKIVKAAHRRRYPYEFLAQQDIEDTGKNRVLLGGIAETLHQRNLAWLYYHTYREN
                     "
     gene            complement(3424..4248)
                     /locus_tag="PK791_01555"
     CDS             complement(3424..4248)
                     /locus_tag="PK791_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92030.1"
                     /translation="MIRNKISVVLSRFSIVSALVALSLTLIYFLPEDVPARVLSIPGL
                     QISFSPLQLIPLFIAIISLTGAYWVLISNPEIERKGWLVQQVLPNLVLPAINIFIFAL
                     ILTQTNKSYEWWGILFTGVLTFSIILYAEYQVLKPNVSTNTIFTVLLISLAHALFMAF
                     TIALRASISRVFLIIPAIVLASIFVSYRTIYLRTDGKFKSYWIYVVTFICFQFAIALY
                     YLFLTPNQYGLILTAILFVSNAVIARIGQGDRKRLYIEPLVIGVFVLAVLLLGNLL"
     gene            4321..5304
                     /locus_tag="PK791_01560"
     CDS             4321..5304
                     /locus_tag="PK791_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92031.1"
                     /translation="MEREQNLLNQPGIIESFKQGYQTVSEKLYLILFPFLFDLFLVFG
                     PKLRIDQLVNSILSALPTPKLAQNLMDQWLTTIEQLKNFTLHYNLFSELRTFPLGIPS
                     LFSWRIFEQSLLSITPVFEIHNEGLFIFLVILFFLLGMGLTTWYYKSIAVSTGLASSK
                     YSFKQFGKNYLHFLGIPITLFLIALGVSLPGMCIISLATVLSPAFASVLYIVLFIVLI
                     SIIIPFIFTPHCVVSHEQKLLDSIKQSRLIYQATKFKTSLFILLAFGLSMLSHLLWNL
                     PKDGSWMLIVGAFGHALVSTIIIVSSFHFFKNAHAYVDNLIKNKVEQGSLA"
     gene            5322..7235
                     /gene="gyrB"
                     /locus_tag="PK791_01565"
     CDS             5322..7235
                     /gene="gyrB"
                     /locus_tag="PK791_01565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOH92032.1"
                     /translation="MSKKIEHYDAGDIQVLEGLEAVRRRPGMYVGGTDVKALHHLIYE
                     VVDNSVDEALAGFCDHIQVIIHEDESVTVKDNGRGIPVDIHPEKKVSALQVVMTVLHA
                     GGKFGGGSYKVSGGLHGVGVSAVNALSEWCEVTVRRDHKIYHQRYEKGISQGDVKVVG
                     TFKENLSGTETTFRYDRTIFLEDATFKFETLANRFREMAFVTRGVSIQLIDERIGKEM
                     TFHFEGGIASFARYVNRNRVPVHPIIKGEKEIDGIGIEFAVQYTDTYSESAYAFANTI
                     NTTDGGTHLTGLRAAITRSINDYARKTSLLKENEPNFSGEDTREGLTCIVSIKHPDPQ
                     FESQTKVKLMNPEVQTYVQQVVIESFGTFLDENPSAGKAIVQKCLTSARARDAAKKAR
                     DLVIRKSALESMTLPGKLADCSERDPQKTELFIVEGDSAGGSAKQGRDRHFQAILPLR
                     GKILNTERARLDKILGNREVRAMISALGTGIGDSFDIEGLRYHRIVIMTDADVDGAHI
                     RTLLLTFFFRYMPELIDAGHLYIAQPPLYRVSSRGNIKYAYDEIQLNEIVKQFGGLSR
                     VNISRYKGLGEMNPEQLWETTMNPENRTMFQVTVENVAEADQTFSMLMGSEVPPRRRF
                     IQTHAKEVRNLDI"
     gene            7269..>7457
                     /locus_tag="PK791_01570"
     CDS             7269..>7457
                     /locus_tag="PK791_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOH92033.1"
                     /translation="MIIRKILHLDLDAFFCSVEELLDPTLVGKAFVVGGNPQGRGVIV
                     SASYAARQFGVRSAMPSGR"
BASE COUNT         2252 a         1666 c         1441 g         2098 t
ORIGIN      
        1 aaaataactg ccaaaaggcc aaaccctggc aaataatcca ttactgttaa tcttcttttt
       61 cccgtagcta atcttgtatc atcaataaca agcggtgttg acaatctacg cgcagttcgt
      121 tgcaataaat aaagcgttgc ccagaaaatt atcgttgaaa ataaactgat taaatcccac
      181 tcccctggac ggttatttgc aaaaactgtg cctgctagca agctcccaaa tccgttaatt
      241 agaagaaacc agtcgatttc caggtctttt aattgaaatt ttttcatgtt tagattttat
      301 catagcgttt tcacccatac attaaatgat ataatgtagc gtcatgaaca aacgcccatt
      361 tattttatct tttcttattt ccgttctttt tatattcggc ttgctaacgc catttatatc
      421 ccgcgatgca gttgatgcaa aaatgcagca tcccctttta tctcaaacag aattgtttgt
      481 tgttaatact tctgaagaat gtgaacctta cgaaactaca ctttgttttt tcaatcacga
      541 aagcctagat gaactcgatg gcgaaggtac aggattgaga gcggcgatta aacatgccaa
      601 agaaaatctt ttatcaggca tcaaaattaa cattgtcgct gattataaaa taaagacttt
      661 tcccgttctc atcgattttc ctgttgttat taatggcttc gataacgcca aaattacaac
      721 ccaggaacca aactgcgacc atgccatgct taatattatc gagtcagtga ctattcaaaa
      781 tctgataatt gatgatggtg cctgttatga cgtttcacgc gacttgatag tggtcaattc
      841 accgaacagt gtcacgatac agaaaaacac actggagaac ggtaaaaatg ctattttgtt
      901 caaggacaat agcggtaatc tctcagtctt ttttaacgat attcgggaca accaactttc
      961 agcaataaga cgtgaagccg gtaccagcac tggcaaactc aaactgactg gcaacatgat
     1021 aacaaacaat aatcctggtg gtaaccaggt cttttgccat cattcaactc ttggtaacgt
     1081 taatcataat tactggggag aggatgtctt tccttcttct gccagcgaaa actgcacatt
     1141 tgacgacaac aaacgtcttg gcgcggagcc ccgacagtta tcagcaggta tcgccggcga
     1201 atatatcaat ttagaaagaa catattcaga tccgattttt ggtggttttt ccgctaagtc
     1261 agaccaaccc ggcaatgcgc tttttgttat tgaccacaat aacaatcaac catttatcga
     1321 taaaatgccc cggaattatg aactcaccca ttgtggcaag ttttatgacg tctttatgga
     1381 tgaaaactca actgcatctt tggtcagttt gcgtttttct tatgataact ctccttgcgc
     1441 tgaagttatt aattcgcagc aattttgtgc ttcaaacaat gaaaaccaat atccactcat
     1501 gtggtttgac cctaaatacg ggagtactgt cacccacggg tatgacaatg tcggtgctga
     1561 accagaagga acaggcagat attttggaca aacggtgcgt tgcaacaccg ctaaccgaac
     1621 aatcgagacc gacatcaaca atgcctttac ccaacgacct aatttaatag atgaccttaa
     1681 ctacacaccc tttacaatcg gatttgataa aacagtcgtt gaagacttta cttcaagcgc
     1741 tgctggtagc acaactatta ctttgacctg gtcaagcttc agtgaagcga atatgacaca
     1801 ttacagactt ttgaaatccg acaaatcgaa tggcgaattc aaaccgatag gcaatgattt
     1861 ttcgcgtaaa ggtgatgcac aatccggcga aaattataaa tatatagata ctggtcttag
     1921 tcccgaaaca acttactatt acaaactagt tgttttcaac ggcgacacaa ttcaacaaac
     1981 ttttggaccg gtcgaagcaa aaaccaatgc ccttgaacca acaaacacgc atacacctac
     2041 gagaactagc acgcaaggac gccctacatc aaccccatat cccatatcga attcacccac
     2101 cccgacgggt tttgtatacc gcagtccaac aagtaccagc acaaaaagct atgtaaaaac
     2161 atctactcca ttgccggaac ttacccaaaa ttcacaacat caaaccatga cggcgttttt
     2221 taccactcaa acagcgcgat actctgaccg tacacctgcc gttgatgaaa ccccatcata
     2281 tgaagacaca tcaacagaaa tcataatatt aaacacatct acagaagagt cccagaatat
     2341 caacgtaacg tttacgccaa gcccaaccca acgcggtgtt aacacagata aatccagtcg
     2401 ttaccacgaa gaacaaagca cgccaaaatg ggtcttttgg cttctcctca ttgtatttgg
     2461 tggatatggt atttatttag gcacgcgtaa aaatattcat taattaaagc cacatcttca
     2521 tctttattta aggtcatttt ttgacaataa ataacgctta tgctatccta ttgttcaata
     2581 caatacgggt caaacgatgc aaaaagaacg cgtttctgat aatgtatatt ggttccaaag
     2641 cgaaatttac gcgcaagtaa cggccggcgc aataattggt ccacaatggg ctgttcttat
     2701 tgacacgctt atgcccgaag aaacccccca catcaaacat tttcttgaat ccgaattaat
     2761 gatgccggtt cgatacgtca tcaacacaca tcatcatgct gatcatagct ggggcaattg
     2821 tttcttccca aatgcgacaa tcatcagcca tgatttgtgc cgtaaataca tgattgaaaa
     2881 gtccatgtcc gcactaaatg aagctgttga aggtggcatg tatttcaaag atatctgtat
     2941 catcccacct gatattactg tcgcagatgg cagtttattc cttcgaatcg gcaaaaaaca
     3001 aattcaaatt tttgccacac ccgggcattc ggacgactca gtagccgttt tgcttgaaga
     3061 agaccgcatt ctcttcgcgg gtgattcctt tatgcccgtt ccctttttcg taggtggaga
     3121 ttatgacgtt ttgctctcta cactaatacg ttatagcgaa atgggcttgg aaaatattat
     3181 ccagggacat ggcgacatta tcttgcgggg cgaaattgat gacaaagtgc agatgaatgt
     3241 tgactatttg catacactaa agaaaattgt caaggcagcc caccgacgtc gttatcctta
     3301 tgaatttttg gcccaacaag atatcgaaga cactggcaaa aatcgtgttc tacttggcgg
     3361 catcgccgaa accttacacc aacgcaattt agcctggctt tattaccata cctaccgaga
     3421 aaattaaagt aagttcccta aaagtaacac cgctaaaacg aatacgccta taaccaatgg
     3481 ctctatatag agtcttttcc tgtctccctg gccaattcgg gcaataactg catttgaaac
     3541 aaacaaaatt gctgtgagta tcaatccata ttgattaggc gttaggaata agtaataaag
     3601 tgcaatagca aactggaaac aaataaaagt taccacatat atccagtaag atttgaactt
     3661 tccatcagta cgtaagtaaa tcgtacgata ggaaacgaaa attgatgcaa gcactatagc
     3721 aggaataatc aaaaaaacac ggctgatcga agcgcgtaaa gcaatcgtaa aagccataaa
     3781 caaagcatgc gccagggaaa taagtaaaac agtgaatatg gtattggtac tcacattagg
     3841 ttttaatacc tgatattcgg catacaaaat gatggagaaa gtcaaaacac cagtaaacag
     3901 aataccccac cattcataac ttttattcgt ttgtgttaat ataagggcga atatgaatat
     3961 attgattgct ggcaaaacca aatttggtaa aacttgctga acaagccagc cttttctttc
     4021 aatttcggga tttgatatca aaacccaata tgcgcctgta aggcttatta tagcgataaa
     4081 taatggaatt aactgcaagg gtgaaaagga aatttgaagg ccaggaatgg ataacacccg
     4141 ggcaggaacg tcttccggca aaaaataaat caatgttaaa cttaacgcaa ctaaggcact
     4201 tacaatgcta aatcggctta atacaacgct tattttattc cttatcataa taagattcta
     4261 tcatgtttga aggcccatta actatcgttt ttacccgata gatggtataa tttaaaggtt
     4321 atggaacgtg agcaaaacct gttaaatcag cccggaataa ttgagtcatt taaacaagga
     4381 tatcaaacag tttcagagaa attgtacctg atacttttcc catttttgtt tgacttgttc
     4441 ttagtctttg ggccaaaact aaggattgat cagttggtaa atagcatttt atcagctttg
     4501 ccaacgccaa aactcgcaca aaaccttatg gatcaatggc ttacaactat tgaacaatta
     4561 aaaaatttta cgcttcatta caacttgttt tccgagttaa ggacatttcc attaggaatt
     4621 ccgtccctgt tttcctggcg gatatttgaa caatcactgc tttctattac tcctgtcttt
     4681 gaaattcaca atgagggatt gtttattttt cttgttattc tttttttctt actgggaatg
     4741 ggcctcacta cgtggtatta caaatcgatt gctgtaagta caggcttagc ctcatccaaa
     4801 tatagtttca aacaatttgg aaaaaactat ttgcattttc ttggcatccc aatcacactt
     4861 ttcctgatag cattaggggt ctccttacct ggtatgtgta tcatcagtct ggcaacagtc
     4921 ctatcccctg cgtttgcaag cgtcctttac attgttcttt ttatagtttt gatctcgatt
     4981 attattccgt tcatattcac cccacactgt gtcgtgagcc atgaacaaaa attattagat
     5041 tcgatcaaac aaagtcgcct gatctatcaa gcgacaaaat tcaaaacgtc attatttatt
     5101 cttttggcgt ttggtctttc catgttaagc cacctgcttt ggaatttgcc gaaggatggt
     5161 tcctggatgc ttattgttgg cgcatttggc cacgcattag tgtccactat aattattgtg
     5221 tcaagttttc actttttcaa aaacgcgcac gcgtatgttg ataatcttat aaaaaataag
     5281 gttgagcaag gatctcttgc ttaacgggtt ggagtgtagc attgagtaaa aaaatagaac
     5341 attacgatgc aggtgatatt caagttttgg aagggcttga ggccgtccgc cgtcgccctg
     5401 gtatgtatgt tggtggtacc gacgttaaag ccctgcatca tttaatttat gaagtcgttg
     5461 acaactcagt tgatgaagca ttagctggct tttgtgatca cattcaggtc attatccacg
     5521 aagatgaaag cgttaccgtc aaagataatg gtcgtggtat tccggttgat atccatcctg
     5581 aaaagaaagt atccgccctg caagttgtta tgacggttct ccacgctggt ggtaaatttg
     5641 gcggtggcag ttacaaagtc agtggtggtt tgcatggtgt cggtgtttcc gctgtcaacg
     5701 ccctttcgga gtggtgcgaa gttaccgttc gacgtgatca caaaatttac caccaacgct
     5761 atgaaaaagg tatctcccag ggagacgtca aagttgttgg tacttttaaa gaaaatttaa
     5821 gtggtacaga aacaactttc cgctacgaca gaaccatttt tcttgaagat gccaccttta
     5881 aattcgaaac gcttgccaac cgtttccgag agatggcttt tgttacccgc ggtgtcagca
     5941 tccagctgat tgatgaacgc atcggcaagg aaatgacctt ccactttgaa ggcggtatcg
     6001 cgtcattcgc ccgttatgta aatcgcaacc gcgtgcctgt tcaccctatt atcaaaggtg
     6061 aaaaagaaat cgatggcatc ggtatcgaat tcgcggtgca atataccgac acatactccg
     6121 aatcagccta tgctttcgcg aacacaatca atactaccga cggtggcacc cacctgactg
     6181 gccttcgtgc cgcgataacc cgcagtatca acgattatgc ccgtaaaacc agcttgctaa
     6241 aagaaaatga accaaacttc agtggtgaag acacacgcga aggcttaact tgcattgtca
     6301 gcattaaaca tcccgacccg caatttgaga gccaaaccaa agtcaaactg atgaaccctg
     6361 aggtgcaaac ctatgttcaa caggttgtca tcgagagctt tggcactttc ctggatgaaa
     6421 acccatccgc tggtaaagct atcgttcaaa aatgtctcac ctctgcccgt gcgcgtgacg
     6481 cagccaaaaa agcccgcgat ttggtcattc gcaagtcagc actcgaaagc atgacccttc
     6541 ctggtaaact ggctgattgt tccgaacgag acccccaaaa gaccgaactt ttcatcgttg
     6601 aaggtgactc agcgggtggt tctgcaaaac aagggcgtga ccggcatttt caggcgattt
     6661 tacccttgcg tggcaagatt ttgaataccg aacgcgcacg tctggataaa attttaggta
     6721 accgcgaagt acgcgccatg atatccgcac tcggtacggg tattggcgat agtttcgaca
     6781 ttgaagggct acgctatcat cgcattgtta tcatgacgga tgccgatgtt gatggcgccc
     6841 acattcgcac cttgctattg accttcttct tccgctatat gcccgaattg atagatgccg
     6901 gccacttgta tattgcccaa cctccactct atcgcgttag ctcacgggga aacatcaaat
     6961 acgcttatga cgaaatccaa cttaacgaaa tcgtcaagca atttggcggt ctgtcacgcg
     7021 tcaatatctc tcgttacaaa ggtcttggtg aaatgaaccc tgaacaacta tgggaaacaa
     7081 ccatgaatcc agaaaaccgt accatgttcc aggtaactgt tgagaacgtc gccgaagctg
     7141 accaaacttt tagcatgttg atgggctccg aagtcccacc acgcaggcgc tttattcaaa
     7201 ctcacgccaa agaggtgcgc aacctggaca tctagtgaaa ggccttgcct gaccttggac
     7261 ttgtcacaat gataatacga aaaattcttc atttagattt agatgccttt ttctgttctg
     7321 ttgaagaatt acttgacccc acactcgtcg ggaaagcttt cgttgtcgga ggcaacccac
     7381 aaggacgtgg ggttattgtt tctgcatctt atgctgcccg ccagttcggc gttcgctcag
     7441 cgatgccatc tggtcgg
//
LOCUS       DAOSFC010000032         7342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_8768_length_7342_cov_4.209688, whole genome shotgun sequence.
ACCESSION   DAOSFC010000032 DAOSFC010000000
VERSION     DAOSFC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8768_length_7342_cov_4.209688"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PK791_01575"
     CDS             <1..420
                     /locus_tag="PK791_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH92034.1"
                     /translation="TSDHLVAERLWGERCQHAHAYKSLHDSGAELFLGTDAPVEIANP
                     FHTLYAAITRQTLAAEPKPNGWYPAQRLNMQEALDGMTVNPARYFGTPAQTGTLKPGS
                     NADFIVLEKDPFTLSPIEMAKIKPVMTWVSGECVYTS"
     gene            563..1288
                     /locus_tag="PK791_01580"
     CDS             563..1288
                     /locus_tag="PK791_01580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH92035.1"
                     /translation="MLKNNEITGMLSCAAESDQAAWLRYFICRRNIDYAEVFNTLIKT
                     AVTSLINARIPHLYALGLPEWFSRLLINQQFTIKHQIISLHLDPSNQIKNLKTNIIDD
                     TTFNIRTMTHDDLISVFQIDQRAFETQWQLGLMNLKACFRASKISLVLTWQNEVLAYL
                     MSEQYFDIQHISRLAVDPAFHGKGIANCLLNTLINEALNNGASQISVNTNDDNTPGLA
                     LYQSLGFVKSDERIPVYALSVLQ"
     gene            1336..2004
                     /locus_tag="PK791_01585"
     CDS             1336..2004
                     /locus_tag="PK791_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOH92036.1"
                     /translation="MTISERELVRSQIQQKKKKNRTIILIILAVIVVVAAVIIITRLP
                     KKGSSFTTEDGLSIGDPNAPVKVLEFTNFSCSHCKNFTLNTSAGFIKQYVETGKVYYT
                     AYPYPWSEDDLTYTASLGAYCAAEQDAFYAYKELVFNRVASPQDLSEEAVRGYAESVG
                     MDLAKFDSCMQDPNLASKVAETKALASTFNVSGTPSFIVNGSLFYANNLIDGVEAALE
                     ASGN"
     gene            complement(2024..2539)
                     /locus_tag="PK791_01590"
     CDS             complement(2024..2539)
                     /locus_tag="PK791_01590"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOH92037.1"
                     /translation="MSRHSKRNPIGYTDLIGASGISKADPRIQANGAVDEASASLGMA
                     RAFMTQKSHADFLKQCQADLSKLMGFIASVNTKQTFFVITDDFFETELQNLENYLFKL
                     EDETEFPRAFVFPGDNQAEGALNLARTIVRRAERELVIFFQNENLKQKAVLQYLNRLS
                     SLCYVLSLIEA"
     gene            complement(2560..4230)
                     /locus_tag="PK791_01595"
     CDS             complement(2560..4230)
                     /locus_tag="PK791_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOH92038.1"
                     /translation="MKQFRWQLLIVLLTALVVGVILILYQNKPESPVIETPSPISGGS
                     YTEALVGQFSRLNPLLDHYNQVDRDVDRLLFSRLIRHNSVGLPVPELAQNWTVSDDAT
                     HYTFFINEKAVWHDGVPVTSHDVAFTVSLIQSGSPYIAQNLREFWPNVEVIVHGDDAI
                     EFVLQDSFAAFFDYLNFQILPSHLIIVQSVEELIDHPFTLSPVGSGPFRFSELIIKEF
                     QIIGVELVANDFYFEGRPFLDKVTFRYYFDRESALQAYLGGEVDGLSSISTNEMNRVL
                     AQPGLNLYSSREAQLSTIFLNNNSTEAPYLSDADFRKALMAAINRQGMIDEVLYGQGI
                     LAVGPFIPDSWAFFDGQPQFRYDPDLARQLIAGLGMKIDGAMKLRDAEGQAVKLQLLV
                     PNDLRYEALASIVERNWKTIGIEVVLDIRPYEEVLSLLEARNYQSALIDVDFSATPDP
                     DPYPFWSEATASEGQNYSGWVNATASEYIEQARVISDYDLRTRLYRNFQVLFAEDLPS
                     LPLFYTVYNYAVKDTIQNVSMGPIYDHSDRFNTICEWYIKTGIPGTEQ"
     gene            complement(4320..4544)
                     /gene="secG"
                     /locus_tag="PK791_01600"
     CDS             complement(4320..4544)
                     /gene="secG"
                     /locus_tag="PK791_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOH92039.1"
                     /translation="MNPLINISMIIVSIFLIISILLQSKGIGLGGLAGGDSGGVYTQR
                     RGIEKVMFYITLALSVVFIILALLSVILTK"
     gene            4689..5028
                     /gene="rnpB"
                     /locus_tag="PK791_01605"
     ncRNA           4689..5028
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK791_01605"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5096..6772
                     /locus_tag="PK791_01610"
     CDS             5096..6772
                     /locus_tag="PK791_01610"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOH92040.1"
                     /translation="MSKKALPIATVALVLTILFAFLLIASCAGKQEPDLSIEISEPKT
                     GEQFYTFQEVFIKSVISGNESWSRVELWVNNQLIRSDTAARIQSNHVTQSWLPLQALP
                     TLLEVRVYNRAGTKYVSDDIAISIIDKPNTLTPEPTPMPGEPTPSNVPTQENCTTAAT
                     LLADLSIPSGTELKPGERFTKSWRIHNNGTCDWVDYKLVYISGSLMGGNSPSLLRTVK
                     ADEVIDIALELVAPSFPGEYTGVWKIQTDKGTLLNTELMYRIIIPAPTATPSPTATQT
                     PTPTPTFTPTATATLVPTATHTATPQPTLEPTPTGPYTLIAMNPQDIARGAAVELSVS
                     CNDVGGQAISGGYTVTDGITVQASQLVENGWSIIAKNNSNQKQRISVHVTCLIDQNLK
                     RQVQSLSATATPNETTELEIDSTGMVAGVGYALDPNNRLTLLTLDSETYKAKMTVHNP
                     TNQEIEFSLQNISLSQSTYQNRTVFTRTDKIKPGETKQINLSCNHGLALGATFENPNN
                     LIITINRPTLNGWLFEVKNNTATEQNFISTLICFDDGSKTIQPIDDFQLQ"
     gene            complement(6810..>7342)
                     /locus_tag="PK791_01615"
     CDS             complement(6810..>7342)
                     /locus_tag="PK791_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92041.1"
                     /translation="LTREAEVLRFQYQPSYDPLASDSNQLFLPVQAAKSFSGVSQQEI
                     ESGLKGAFVKNRRQDLARGVTTIGPHRDEFRFLSNGIDLGYYGSRGQTRSALLSLKFA
                     EVAWMKSRIGDTPVLLLDEIMAELDTERRNDLLNLIEDVEQAMLTTTDKSMFATEFVS
                     ACDTWDVHEGIVKKDA"
BASE COUNT         2215 a         1772 c         1437 g         1918 t
ORIGIN      
        1 acatctgatc accttgtcgc ggagcgtctt tggggcgagc gttgccagca tgcccatgcc
       61 tataaatcac tccatgacag tggcgcggaa ttgtttctcg gaaccgacgc ccccgttgaa
      121 attgccaacc ctttccacac cttatacgct gccataacac ggcaaacact cgcagctgag
      181 cccaaaccaa acggctggta ccctgcccag cgattgaata tgcaagaagc cttagatggc
      241 atgacggtca accctgcccg atattttggc acaccagcac aaaccggtac gctcaaaccc
      301 ggttcaaatg cggattttat cgttcttgaa aaagacccgt tcacattatc acccatagaa
      361 atggctaaga tcaaaccggt gatgacctgg gtctccggtg aatgtgtcta caccagctaa
      421 gctgatgtca ttttccattt atccttccag cgaatttgac cttttgtctc agtatgaatt
      481 tttaaacagc aatcatctgc gctataagca cttggactgg gtgcccgaga aacaacgtct
      541 gattgaaccc ttcgctttta ccttgcttaa aaacaacgaa ataacaggta tgctgtcttg
      601 tgctgctgag agcgatcaag cagcctggtt gcgttatttt atatgcaggc gaaatattga
      661 ctatgcggaa gtatttaaca cactgataaa aactgcggtg acatccctta ttaatgcaag
      721 aatcccgcat ctttacgcct taggtttgcc tgaatggttt agccgtcttc tcatcaatca
      781 gcaatttaca atcaaacatc aaattatctc gcttcatctt gacccatcaa accaaattaa
      841 aaaccttaaa actaatatta tcgatgatac aactttcaat atccgcacta tgacccatga
      901 tgatttaata tcagttttcc aaattgacca acgcgctttt gaaacccagt ggcaattagg
      961 attaatgaac ttaaaggctt gttttagagc gagcaaaatc agtctggttt taacatggca
     1021 aaatgaagtc cttgcctatt tgatgagtga gcaatatttc gacattcagc atatttcccg
     1081 tctggctgtt gacccagcat tccacggaaa aggtatcgcc aactgcttac ttaatacact
     1141 tatcaatgag gcactaaaca atggagccag ccaaatcagt gtcaacacaa atgatgacaa
     1201 tacaccaggt ttggctttat accaaagcct gggttttgtg aaatctgatg aacgtattcc
     1261 cgtttatgcc ttatctgtat tacaataatc ctaccgttat tgtacggttg aaatcaaaac
     1321 acaaagaggt taaccatgac aattagtgaa cgcgaactgg tacgttctca aattcagcaa
     1381 aaaaagaaaa agaaccgaac cattattctc ataatcctgg cagttattgt agtagttgca
     1441 gcggttatta tcatcacccg tcttcccaag aaaggttcaa gttttacaac tgaagatggc
     1501 ttgagtatcg gtgatccaaa cgcaccggta aaagtgcttg aatttaccaa tttttcatgc
     1561 agccactgta aaaactttac actcaacact tctgccggct tcatcaaaca atacgtcgaa
     1621 acaggcaaag tttactacac agcctatcca tatccgtgga gcgaagacga cctgacttac
     1681 accgcgtctc ttggtgcgta ttgcgcggct gaacaggatg ctttttatgc ctataaggag
     1741 cttgttttta accgtgtagc cagcccgcag gatttgagtg aagaagctgt gagaggatac
     1801 gctgaatctg tcggtatgga tttagcaaag ttcgattctt gtatgcaaga tccaaacctg
     1861 gcatcaaagg ttgcagaaac caaagcgctt gctagcacct ttaatgtatc cggtacacca
     1921 agttttatcg taaatggcag tttgttctac gcaaacaatc tcatcgatgg tgttgaagct
     1981 gctttagaag cgagtggcaa ttaatatcca tccaacgata agtctaagct tcaatcaggc
     2041 ttagtacata acacaaactc gaaaggcggt tgagatattg caaaaccgcc ttttgtttta
     2101 agttttcatt ctggaaaaat ataaccagtt cccgttcggc acgtctcaca attgtccttg
     2161 ccaaatttaa agcaccttct gcttggttat cgccaggaaa aacgaacgcc cttggaaatt
     2221 ctgtttcatc ttccaatttg aacaaataat tttcaagatt ctgcaattca gtctcaaaaa
     2281 aatcgtcagt tatgacaaaa aaagtttgtt tggtattaac acttgcaata aatcccatca
     2341 gtttggaaag atctgcttga cactgtttta gaaagtccgc atgggacttt tgagtcataa
     2401 acgcacgcgc cattcccagt gatgcgctgg cctcatctac tgcgccatta gcttgaatgc
     2461 gtggatcagc cttgctaatt cctgatgcgc caattaaatc agtataacca ataggatttc
     2521 gtttcgaatg tcttgacatg gcgcacctct tttgtctttc tattgttctg ttccaggaat
     2581 tcctgttttt atataccact cacatatggt attgaaacgg tcactgtggt cataaatcgg
     2641 ccccatagat acattttgaa tcgtatcctt tacagcataa ttgtaaacgg tgtaaaacaa
     2701 gggcaaggat ggtaaatctt ccgcgaaaag cacctggaaa ttacggtaaa gcctggttcg
     2761 aagatcatag tcactgataa cacgcgcttg ttctatatat tcactggcag tcgcattgac
     2821 ccaaccagaa taattttgcc cttcagaagc tgtggcctca ctccaaaatg gatagggatc
     2881 agggtcaggc gtggcagaaa aatcaacatc aatcaacgcg gactggtaat tccgcgcttc
     2941 caatagcgac aaaacttctt cataaggtct aatatccaga acgacttcaa taccaattgt
     3001 tttccaattt cgttcaacaa tactggcgag tgcttcgtag cgaagatcat ttggaaccaa
     3061 cagttgcaac ttcactgctt gtccttcagc atctcttaat ttcattgcgc catcaatctt
     3121 catccccaac cctgcaataa gttgtcttgc caggtccgga tcatatctaa attgcggttg
     3181 gccgtcaaaa aacgcccagc tatcgggaat aaaaggtcca acagccaaaa taccctgtcc
     3241 gtataagacc tcatcaatca tgccctggcg attaatagca gccataagtg cttttctaaa
     3301 atccgcatct gaaaggtatg gcgcttcagt actgttattg ttgaggaata ttgtactcaa
     3361 ttgagcttcc cggctagaat ataagttcaa accgggctgt gccaacacac gattcatctc
     3421 gttggtagat atactcgaca agccatcgac ttcgccgccc aaataggctt gcaaagcaga
     3481 ttcgcggtca aagtaatacc ggaaagtaac tttatcaaga aaaggccggc cctcaaagta
     3541 aaaatcattc gccactaatt caacaccaat aatctgaaat tccttgatga ttaattctga
     3601 aaaacgaaaa ggtcctgaac caacaggcga tagtgtaaat ggatggtcta tcaattcttc
     3661 aacgctttgc acaattatta gatgcgatgg caatatttga aagttgagat agtcaaaaaa
     3721 agccgcgaaa ctgtcctgta agacaaactc aatcgcgtcg tcaccatgca caataacttc
     3781 cacattaggc caaaattccc ttaggttttg tgcgatatat ggacttccgc tttgaatcaa
     3841 gctaaccgta aaagcgacat catgactggt cacgggcaca ccatcatgcc aaaccgcctt
     3901 ttcgtttata aaaaaggtgt aatgcgtggc atcgtccgaa accgtccagt tttgagccag
     3961 ttccggaaca ggcagaccaa cactgttatg ccgaattaat cggctaaaca acagtctgtc
     4021 cacatctctg tccacttggt tgtaatggtc caatagtgga tttaaacggg aaaactgccc
     4081 cactaatgct tctgtatagc ttccaccact aatggggctg ggcgtttcga ttacaggact
     4141 ttccggtttg ttttgataga gaatcaaaat gacgccaacg actaacgccg ttagcagaac
     4201 gataagtaat tgccagcgaa attgtttcat attagaaaaa agacgacctt cgcacctggc
     4261 gagtcgtctt attcctccat ataatagaat tgtcgctacg ccattaaagc atacgcgact
     4321 tacttagtca aaatgacact tagcaatgcc agaatgatga aaacaacact taaagcgagt
     4381 gtgatataaa acatcacttt ctcaataccg cgtcgctgag tgtaaacacc accggaatcg
     4441 ccacctgcca gaccaccgag tccaattccc ttactttgca gcaaaatgct gatgataagg
     4501 aaaatagaaa cgataatcat cgaaatatta attaaagggt tcaattctgc ctcctacaat
     4561 atgcatcatt gaattatact caaggattag aaatcgtcaa ctttttttga atattatccc
     4621 tgcttcttag ctcgaattac cttgaaactt tagacatagc cactcaattc tgctatcatt
     4681 aaaccgttca ggttggttga atgtgcgcgc tcttcgcaga gtgaggaaag tccgtacacc
     4741 acagagcagg atgccaggta acacctggag aggcgtaagc ttttggatta gggccacaga
     4801 aacataccgc gttgtaatac gtaaggttga aaaggtggtg taagagacca ccggcgggtt
     4861 tggtaacaag cccggccagg taacccccat ccggtgcaag gtcaaacagg aaagagagtt
     4921 ggctcggctc attagatttc cgggtagacc gcaagagcag ttcggtgacg tcctgcccag
     4981 atagatgcgc attctcgaca gaatacggct tacagaccaa cctgaacagg gaacttttag
     5041 cataccaaaa acgttttata gttgcatttt tgatgatgct atggagaaaa ccattatgag
     5101 taaaaaagcc cttccaatcg caacagtcgc attagtgctt acaatactgt ttgcctttct
     5161 tttaattgca tcctgtgccg gtaagcagga accagattta tcgatagaaa tttcagaacc
     5221 taaaaccggc gaacagtttt atacatttca ggaagttttc atcaaatcgg tcatttcagg
     5281 caatgaaagc tggtcgcgcg ttgaactatg ggtcaacaat caacttatcc gcagcgatac
     5341 agcagcccgt attcaaagca accacgttac gcaatcctgg ttgccattgc aagctttgcc
     5401 taccctgctt gaagtccggg tttacaatcg ggctggaaca aaatacgtca gcgatgacat
     5461 cgcaatatcc atcatcgata aacctaacac ccttacgcct gagccgacgc ctatgcctgg
     5521 cgaacccaca ccgagcaatg tcccgaccca ggaaaactgc actaccgcgg caaccttact
     5581 ggcggattta agtattccaa gtggaactga actaaagcct ggcgaaagat ttaccaaaag
     5641 ctggcgtatt cacaataatg gtacctgcga ctgggttgac tataaactgg tttacatcag
     5701 tggcagcctt atgggtggca attctcccag tctgttgcgg actgttaaag ctgatgaagt
     5761 gattgatatc gcccttgaac tcgtggcgcc aagtttcccc ggtgaatata ctggcgtttg
     5821 gaaaattcaa accgacaaag gcacacttct taacaccgaa ctgatgtatc gcatcatcat
     5881 tcctgcgcca accgctacac ccagcccgac cgcgacccag acaccaaccc caacacctac
     5941 ctttacacca acagcaaccg ctacgcttgt gccaactgca actcacacag cgacaccaca
     6001 accgacacta gaacccactc caaccggtcc atacacacta attgccatga acccccagga
     6061 tatcgccagg ggagcagcag ttgaactttc ggtatcttgt aatgacgttg gcgggcaagc
     6121 cattagcggc ggttacactg ttacggatgg gattaccgtg caagccagtc agcttgttga
     6181 aaatggatgg tcaatcattg ctaaaaacaa cagcaatcaa aaacagcgaa tcagcgttca
     6241 cgttacctgt ctgattgacc aaaatcttaa acgtcaagta caaagcctga gcgcgactgc
     6301 gacacctaat gaaaccacgg aacttgaaat tgacagcact ggcatggtag caggcgtcgg
     6361 ttacgcttta gaccccaaca atcgtcttac acttctcacg cttgacagcg aaacctataa
     6421 agcaaaaatg actgtacata acccgactaa tcaagagatt gaatttagct tacaaaatat
     6481 cagtctcagt caaagtacgt accaaaaccg gactgttttc acacgaacag acaaaatcaa
     6541 acctggagaa accaagcaaa ttaatcttag ttgcaatcac ggattagccc ttggcgcgac
     6601 atttgaaaat cccaataatc tgattatcac catcaatcgc ccaacgctga atggatggct
     6661 atttgaagtc aagaataata ccgctaccga acagaacttt atcagcacac tgatatgctt
     6721 tgatgatggc agtaagacaa tccagccgat tgatgatttt caattacaat aggaaaaaat
     6781 atatccagag aaaaggacgc ttgaaaattt tatgcgtcct tttttactat gccttcatga
     6841 acatcccatg tatcacaggc tgagacaaat tcagtcgcaa acatactttt atcggttgta
     6901 gttagcatcg cctgttccac atcctcaatc aggttcaata gatcattacg ccgttctgtg
     6961 tcaagctcag ccataatctc atccaaaagc aaaacaggcg tatccccaat acgacttttc
     7021 atccaggcaa cttcagcaaa tttcagggaa agcaatgcgc ttcttgtctg accccgtgaa
     7081 ccataatatc ccaaatcaat cccgttgctt aaaaaccgaa attcatcgcg atgtggccct
     7141 atcgttgtaa caccgcgtgc cagatcctga cgccgatttt tcacaaaggc acccttcaaa
     7201 cccgattcaa tttcttgttg gcttacacca ctaaaacttt tcgccgcttg cacaggtaag
     7261 aaaagctgat ttgaatcgct agccaaaggg tcataactgg gctgatattg aaagcgcaaa
     7321 acttcagctt ctctcgtcag cc
//
LOCUS       DAOSFC010000033         7308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_8833_length_7308_cov_5.569282, whole genome shotgun sequence.
ACCESSION   DAOSFC010000033 DAOSFC010000000
VERSION     DAOSFC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8833_length_7308_cov_5.569282"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PK791_01620"
     CDS             complement(<1..384)
                     /locus_tag="PK791_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92042.1"
                     /translation="MEARQHNDHFERFRLKNSEQRFILILGDFLAGTIALFLTLFIWA
                     QIDSLDFSAAFFRDRVDVWFYFLPIFWVILLIDTYDLGKAGKFKSTLKGVIIALAIAV
                     AIYLVVYFLVPPMSLPRVGVAIFIIV"
     gene            complement(403..1389)
                     /locus_tag="PK791_01625"
     CDS             complement(403..1389)
                     /locus_tag="PK791_01625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH92043.1"
                     /translation="MQKTVSVVIPCLNEEKTIRQVLDALRAQSYPLELMEIVIADGGS
                     KDRTREFIKTYQTEYPEQKIKLVDNPKRIIPAGVNVAMLASVGEIIVRMDAHSIPQND
                     YVARCVAGLEAGLGDNVGGRWDIMPSGTTWIAKSIALAAAHPIGVGDAQYRHSDTAAA
                     VDTVPYGSFYRALLDKVGLFDETLIANEDYEFNTRIRQSGGTIWFDPQIRCQYFARPN
                     LSRLAKQYWTYGFWKFKMLQRYPSTIRWRQFLPPAFLLGLIFLAVLSIFFKPAVYALT
                     AVLGIYLLVLLLVGLSQGNKEKNIALVLGVPLAIATMHFSWAAGFIASIFKK"
     gene            complement(1421..1843)
                     /locus_tag="PK791_01630"
     CDS             complement(1421..1843)
                     /locus_tag="PK791_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002886926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOH92044.1"
                     /translation="MKKCHSKEIITCDKVAPAIGPYSVGVKTGHLLFVSGQLGFDPET
                     ALLVEGGVEAQAHQALKNLRAILESAGSGLENVLKTTVFLMDMGDFAKVNAIYAEYFT
                     ENPPARSAVQVAALPKGGLVEIEAIALVPRNEEADCSK"
     gene            complement(1956..2273)
                     /locus_tag="PK791_01635"
     CDS             complement(1956..2273)
                     /locus_tag="PK791_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92045.1"
                     /translation="MSILTWIFGEEAIRRLPAQQKAEIRQKIDQLVHIGKTDDFLSLV
                     PGGPFNYQCHHREARTIGEEINAMGGLALMMAVRQTIKRKLSDVMAEHLDHAWKGIGD
                     WQP"
     gene            complement(2376..4157)
                     /locus_tag="PK791_01640"
     CDS             complement(2376..4157)
                     /locus_tag="PK791_01640"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOH92046.1"
                     /translation="MMMQKRKIAQWLIVLVFFLASACGSQITPPPTPAAGEMALGIAT
                     VTPKITEPSPEPAVQTTPTADLTAVETEQVVENPSICSPTDQSLPVSLKSKLLDMINH
                     DPAPDTDEPCTTIVPGDDRQIGQMVLTLVAPFPTVTDEIETVSLKSFWQTGDNTFIDR
                     LLVYPYGMEALTWLWGEPKGTVELMPHTVQDIDFADIQMDMIKEAWISERTWAIVAFD
                     ELHPRWKVIALDGQSPIQKSFDPETYALTIPLTLYNENPTRLERSFEYFAYPKFEDLM
                     PFSNYNPERLTTVILTGVTAMVRATAVGMDERGIHVPGEDLADVLKEADILHISNEVP
                     FAENCPKHYRGNYLVFCTPESYMDLLRSIGTDVVELTGDHFQDYGPEAMLYTLELYAK
                     EGWPVYGGGKDIFDAREPVKMEVNGNKIAFLGCNAKSPNFAQASETSGGAYHCDMDYM
                     AETVRELRNEGYLPIVTFQHEERYEWSPNAQMIRDFGIVLEAGAVITSGSQAHQPHYA
                     EFVHDGFAHYGLGNLFFDQYGVEEYTDWAFIDRHVFYNGKHISTEVLTIRFLDLVKAT
                     WARSEQRVEMLEMLFDTARMRWQDDEP"
     gene            complement(4340..4858)
                     /locus_tag="PK791_01645"
     CDS             complement(4340..4858)
                     /locus_tag="PK791_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92047.1"
                     /translation="MNQQEANHSQIELKKAQHFANFKNGVSWFFWVAVISVINIVIRI
                     SNADSPIRFAVGFSITNWLDAHPLPILANASPRVITIVVGFAFAIVLIVFGLLARKRN
                     RVAYLAGTLLYALDTVIAFLMRDVYAIMFHLIVLGFLVWGIINLFKLEKLEAEYPDKT
                     EPDEIVIGPDIA"
     gene            complement(4940..5914)
                     /locus_tag="PK791_01650"
     CDS             complement(4940..5914)
                     /locus_tag="PK791_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH92048.1"
                     /translation="MTEQEKSNLSSTQARGRGFFYAVVAAILFAFTPLFIKILNTRFG
                     VPALVITMWRNILGIALLFPAFLIFKPSLLKLERKHFGFIALYGVVFTLLNTIWTFSI
                     QYNSASVATVLIYFSAPLTSLLGWWLLKERFSWLKGLAIVLSLAGCALVALTGSEMET
                     NWIGLLLGLLSGLIYAFYAICGRFSAQRGINPWTSTFYIFFFALVALFVLNVLPIEIP
                     GQAPDMKTLFTLCTDPMAWLFMVLLTVPLVVGYGLFNQSLTILPSSTANLILTLEPPV
                     TAVLAWLMLKEKLLPLQLLGTVLVLSGVLLVRLGADKPLKTTDKTKTV"
     gene            6077..>7308
                     /locus_tag="PK791_01655"
     CDS             6077..>7308
                     /locus_tag="PK791_01655"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HOH92049.1"
                     /translation="METTQKMNLEQNKPQNPHGYARRGKQIAFSLFVGLFIATLTLVI
                     IAFRYEAKYKDKIYPTIRANEIDLTGLSLGQAVSRLNTDLNYPTKGKIVFSHNNQQWT
                     FTPQELGYSNNPTELAIRAYQTGRDGNWLQNLQSKVFARRQGIELNPSAMFDQPVAFA
                     KLEEIAREINQPLVEAGISIEGTQVTVHDGQIGLTLDIPATLDKLQAYFLIQQDANIP
                     LIVRENSPEILHAGDIGEQAQAILSAPLTLRHPDPDSGLEPWVIPAEDLASLLIIERD
                     TSSQTAGYKLDINATSLEHYLSSIAAKFVVQPENARMRFNEDTRQFELIRNAVMGKVL
                     DVEASLDVILHNINNHQHEVNLVLKDLAPRVTDTTTAAELGITELVVQETSYFYGSEA
                     ARVQNIQVGSSTFDGILIA"
BASE COUNT         2150 a         1850 c         1510 g         1798 t
ORIGIN      
        1 gacgatgata aaaattgcca cgcccactct gggtaaagac atcggtggta ccaggaagta
       61 aaccaccaaa tagatcgcca cagcaatcgc taacgcgatg atgacgcctt ttaaagttga
      121 cttaaattta cccgctttgc ccaaatcgta tgtatcaatg agtaagataa cccaaaatat
      181 gggcaaaaag taaaaccaga catccacacg gtcgcggaag aaagcagccg agaaatcaag
      241 tgagtctatt tgcgcccaaa tgaacaaagt taaaaatagc gcgattgttc cagccaaaaa
      301 atcgccaagt atcaaaataa aacgttgttc agagtttttt agcctgaacc gttcgaaatg
      361 atcgttatgt tgtcgggctt ccatattaag actcctaaaa ttttattttt taaaaatgct
      421 ggcaataaac cctgccgccc atgaaaaatg catcgtcgca atcgccaggg gaacacccaa
      481 aacaagagca atattttttt ccttatttcc ctggcttaat cctacaagca acagtaccaa
      541 taaataaatc ccaagcacag ctgtcagtgc ataaacagca ggtttaaaga atatgctcaa
      601 aaccgccaga aaaatcagcc ccaataaaaa ggctggtggt aaaaactgac gccagcgaat
      661 tgtgctcgga tagcgttgaa gcatcttaaa cttccaaaaa ccatacgtcc aatattgctt
      721 ggccaggcga ctcaaatttg gtcgcgcaaa atactgacat cgaatttgcg ggtcaaacca
      781 aatcgtcccc ccagattgac gtatgcgcgt gttgaactcg tagtcttcat tggcgatgag
      841 cgtttcatca aacaagccca ctttgtccag taaggcacgg taaaaagaac cgtaagggac
      901 ggtgtcaacc gctgcagcag tatcactatg gcgatattgc gcgtcgccca caccaatcgg
      961 atgcgcagca gcaagggcaa ttgatttcgc aatccaagtc gttcccgatg gcataatatc
     1021 ccatcgtcca ccgacattat cgccaagccc tgcttccagt cccgccacac agcgcgccac
     1081 ataatcattc tgcggaattg aatgagcatc catacgcacg ataatttcac ccaccgatgc
     1141 cagcatcgcc acgttcacac cagcggggat aatgcgcttt ggattgtcaa ccagcttaat
     1201 tttttgttca ggatactcag tttgataagt tttaataaat tcgcgcgtgc ggtcctttga
     1261 cccaccatcc gcgatgacaa tctccatcag ttcaagcgga tagctttggg cacgcaaggc
     1321 atcaagcacc tgacggatag ttttctcctc attcaggcaa ggtatgacga cggagacagt
     1381 tttttgcata tttccttacg gaacaacaaa ctgactgcga ctatttgcta cagtcagctt
     1441 cttcattgcg gggaacaagc gcaatggctt caatttcaac caagccaccc tttggcaaag
     1501 ctgccacttg cacagctgac cgtgctggtg ggttctcggt aaagtactca gcataaatag
     1561 cgtttacttt tgcaaaatcg cccatgtcca tcaaaaagac tgtggttttt aagacatttt
     1621 caagaccgga gccggcactt tcaagaatag cgcgcaggtt ctttagcgcc tgatgtgcct
     1681 gggcttccac accaccttca accaaaagcg cggtttctgg gtcaaatcca agttgcccgg
     1741 aaacgaaaag caaatggcct gttttcacac caacagaata aggtccaatt gcgggcgcaa
     1801 ctttatcgca agtgatgatt tctttgctat gacatttttt catagttaat aaacctttct
     1861 tgaattgtat tatccgtgat tatatcactg cacgggcttt catgccccaa aacagctttt
     1921 atatacgcat tgccctgtga agagcaatac atggactacg gctgccagtc gccgatgccc
     1981 ttccaggcgt ggtccaaatg ctccgccatg acatcactca atttgcgctt gatggtctgc
     2041 cgaacagcca tcattaaagc taagccaccc atggcgttga tttcttcgcc tattgtgcgg
     2101 gcttcccggt gatggcactg ataattaaaa gggccacccg gcacgagcga caaaaaatca
     2161 tccgttttgc caatatgcac caactggtca attttttgtc tgatctcagc tttttgctga
     2221 gcaggcaacc tgcgaatcgc ctcttcccca aaaatccaag ttaatatact catgtcattg
     2281 cctttttctt ttattatact tgcaagatga atgcctggtt agggctttaa ccatgagttt
     2341 tgtcatcatt taggtcattt ttttcattct gttcgtcaag gttcatcatc ttgccatctc
     2401 atgcgagccg tgtcaaaaag catttccagc atctcaacgc gttgttcaga ccttgcccaa
     2461 gtggctttta ccagatccaa gaaacgaatg gtaagcactt ccgtggaaat atgctttcca
     2521 ttataaaaga catggcggtc aataaacgcc cagtctgtgt attcttccac accatactgg
     2581 tcaaaaaaca aattgcctaa accgtaatga gcaaaaccat catgcacaaa ttcagcataa
     2641 tgtggctgat gagcctgact gccactggta ataacggcac ccgcttccag cacaatgcca
     2701 aaatcgcgaa tcatttgcgc atttggagac cactcataac gttcttcgtg ttgaaaagtg
     2761 acgattggca gatagccttc atttcttaat tccctcactg tttctgccat gtagtccata
     2821 tcgcagtgat aagcgccacc ggatgtttcg cttgcctggg caaaattagg cgacttggca
     2881 ttgcaaccca gaaaagcgat tttattgcca ttgacttcca ttttgactgg ctcgcgggcg
     2941 tcaaaaatat ctttgccacc accataaacg ggccagccct ctttggcgta aagctccagg
     3001 gtataaagca tcgcttcagg gccatagtcc tgaaaatgat cacccgttag ctcaaccacg
     3061 tccgtgccaa tcgagcgcaa aaggtccatg tacgactctg gcgtacaaaa gaccaaatag
     3121 ttaccccggt aatgcttggg acaattctcc gcgaagggaa cttcattgct gatatgcaga
     3181 atatcagctt ctttcaaaac atctgccaaa tcttcgccag gtacatggat gccacgttca
     3241 tccataccca ccgcggtggc acgcaccatc gcggttacgc cggtaagaat gacagtggtt
     3301 agcctttcag gattgtagtt gctaaaaggc attaaatcct caaactttgg atatgcgaag
     3361 tattcaaagc tacgttccag acgtgtagga ttctcgttat aaagcgttaa cggaatagta
     3421 agtgcatagg tttcggggtc aaaacttttt tgaattggtg attgaccatc cagggcaatc
     3481 actttccagc gcgggtgcag ttcatcaaat gcgacaatcg cccaggtacg ttcgcttatc
     3541 caggcttctt ttatcatatc catttggata tccgcaaaat caatatcctg gactgtgtgt
     3601 ggcatcaatt caaccgttcc ctttggctct ccccataacc aggtgagagc ttccatgcca
     3661 taaggataga ctaaaagccg gtctatgaaa gtattatccc cggtttgcca aaaagatttc
     3721 agcgaaaccg tttcgatttc atcagttaca gttggaaaag gcgcaaccag agtcaaaacc
     3781 atttgaccga tctgacggtc atcaccagga acaatggtcg tacacggctc atcagtgtca
     3841 ggtgcaggat cgtgatttat catgtcaagc aattttgatt tgagcgatac cggtaaagac
     3901 tgatctgtcg gcgaacatat actggggttt tccaccactt gctcggtttc tactgctgtc
     3961 aaatccgctg taggcgtggt ttggactgcc ggctcaggtg atggttcagt aattttaggc
     4021 gtaacggtag caattcccag cgccatctca cctgctgctg gtgttggtgg cggagtgatt
     4081 tgcgaaccgc aggcactcgc caggaaaaaa acaagcacaa tcagccattg cgcgattttt
     4141 cgtttttgca tcatcataaa attttcctta tccttgcttc gtttgcatta tacgtcaatt
     4201 ttccagaccg gttttcatta gcaatgcagg tcatcaagtc aggcaataaa aaacaagcca
     4261 acgaaactat tcatgagctt gttcttattt atcattaaac aacattcctg ccatttagca
     4321 aggtgaatat tttttaagtt taggctatat ctggtccaat cacgatttcg tcaggctctg
     4381 ttttatcggg atattcagct tcaagtttct caagtttgaa caaattgata atgccccaca
     4441 ccaaaaaacc caacacaatc aggtggaaca tgatggcata aacatctcgc attaaaaacg
     4501 cgatgaccgt atccaaagca tatagcaaag tgccagcaag ataagccaca cgatttcttt
     4561 tacgcgccag caaaccaaaa acaatcagaa cgatggcaaa agcaaagccc acaactatag
     4621 taatcactct tggcgatgca ttggccaaaa tgggcaaggg atgagcatct aaccagtttg
     4681 taattgaaaa cccaaccgca aagcggatgg ggctatccgc gttactaatg cgaatgacga
     4741 tgttaatcac cgaaatcact gcaacccaga aaaaccagga gacaccattt ttgaaattgg
     4801 caaagtgttg cgcctttttc agttctattt gactgtgatt agcttcttgt tgattcatct
     4861 tgacctccag ataccttatc catcagaaac ttaattgtaa taccaagtat aaaaaacgtc
     4921 tgttttaatg acgcgatgtt tacacagttt tagttttatc cgtggttttt aagggcttgt
     4981 ccgcgccaag tcgcaccagc aaaacccccg aaagcaccaa aaccgtaccc agcagttgca
     5041 aaggcaagag tttctctttt agcatcaacc aggctaaaac tgccgtaacc ggtggttcga
     5101 gcgtcaaaat caaattcgcg gtactggatg gcaaaatcgt caggctttga ttaaacaaac
     5161 cataaccaac caccagagga acggttagca gaaccataaa cagccatgcc atcgggtccg
     5221 tgcacaaggt aaacaaagtt ttcatatcag gagcttgccc tggaatttcg atgggaagaa
     5281 cattcaaaac aaacaatgcc accaaagcaa aaaagaaaat gtaaaacgtg cttgtccacg
     5341 gattgatgcc acgctgagca gaaaaccggc cacagatagc ataaaaagcg taaatcaaac
     5401 cagacaacag ccccaaaagc agaccaatcc agttggtttc catttcactg ccagtcagcg
     5461 cgaccaaagc acaccctgcc agactaagaa caattgccag accctttagc cagctaaacc
     5521 tttccttaag cagccaccac cccagcaaac tggtcaatgg cgcggaaaaa taaatcagca
     5581 ccgtggcaac ggaagcactg ttgtattgga ttgaaaaggt ccaaatggtg tttaacaaag
     5641 taaagaccac gccataaagc gcgataaaac caaaatgctt gcgttccagc ttcaaaagtg
     5701 atggtttaaa aatcaggaaa gccggaaaca gcaaggcaat cccaagaata tttcgccaca
     5761 tggtaatcac taaagctggt acaccaaagc gggtgttgag aatcttaatg aagagcgggg
     5821 tgaaggcaaa aagaatcgct gcgacaaccg cataaaaaaa gccacggcca cgggcttgtg
     5881 ttgatgatag gtttgatttt tcttgttctg tcataaaaca agtttatcat gcctgttttg
     5941 tttcaagcca ttaaaaggct ttgtggaaga aactggaact ttttcacggc taaatacgtt
     6001 ttgatattaa agcaaacagt taatctgtct tgccaggttg gaagattaat gacgactaaa
     6061 ctataatttg agcgttatgg aaacaacaca aaaaatgaat cttgaacaaa acaaacctca
     6121 aaacccacat ggctacgcca ggcgtggcaa gcaaattgcc tttagccttt ttgttggctt
     6181 gtttatcgcc acactaacgc tggtcattat cgcctttcga tacgaagcca aatacaaaga
     6241 taaaatctat cccaccatcc gcgccaacga aattgaccta accggcttga gccttgggca
     6301 ggccgtctcc cgcttgaata cggacctaaa ctatccgaca aaagggaaaa tcgtttttag
     6361 ccacaacaat caacaatgga ccttcacacc gcaggaactc ggttacagca acaaccccac
     6421 cgaacttgcc atacgcgctt atcaaacagg acgtgatggc aactggctgc aaaacttgca
     6481 aagcaaagtt ttcgccaggc gtcagggcat cgaattaaac ccaagcgcga tgttcgatca
     6541 gccggtggcg tttgccaaac tggaagaaat cgcccgggaa atcaaccaac ccttggttga
     6601 agccggcatc agcatcgaag gcacccaagt caccgtacac gatggacaaa ttggcctaac
     6661 cttggatatc ccagccacgc tggacaaact tcaggcctat tttctgatac aacaagatgc
     6721 caacatcccc ttaatcgttc gcgaaaacag cccagagata ctccatgcag gcgatatcgg
     6781 tgagcaagcc caggccatcc ttagcgcgcc ccttaccctg cgccatcccg accccgactc
     6841 aggccttgag ccctgggtta tccccgcgga agaccttgcc agtctcctga tcatcgagcg
     6901 cgacacgagc agccagaccg cgggctataa actcgacatc aacgctacat cactggaaca
     6961 ctatctttcg agcatcgctg ccaaattcgt ggtgcagccc gaaaacgccc gcatgcgctt
     7021 caacgaagac acccgccaat ttgaactcat ccgcaatgcc gtgatgggca aggtactcga
     7081 cgttgaagcc agcctggacg tcatcctgca caacatcaac aatcatcagc acgaagtcaa
     7141 ccttgtgctc aaagaccttg caccgcgcgt cactgatacc actaccgccg ctgaactggg
     7201 tatcaccgaa ctcgtggtac aggaaacatc ctacttctat ggttcagaag ctgcccgtgt
     7261 ccagaatatt caagtcggct caagcacttt tgatggtatt ctcatcgc
//
LOCUS       DAOSFC010000034         7295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_8855_length_7295_cov_6.021823, whole genome shotgun sequence.
ACCESSION   DAOSFC010000034 DAOSFC010000000
VERSION     DAOSFC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7295
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8855_length_7295_cov_6.021823"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2062)
                     /gene="fusA"
                     /locus_tag="PK791_01660"
     CDS             complement(<1..2062)
                     /gene="fusA"
                     /locus_tag="PK791_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH92050.1"
                     /translation="MTEFPLDRYRNIGVIAHIDAGKTTTTERILFYTGKTYRIGNVDD
                     GTTVTDWMPQERERGITIVSAAVTAEWKGYRLNLIDTPGHIDFTAEVQRSLRVLDGGV
                     VVFDSVQGVEPQSETVWRQADKYAVPRIAFANKMDRTGASYERTIESIRERLGANAVQ
                     MQIPLGVEAGFTGVINLLEQDAWYFEDKVGSEPRITAVPEEYREEVALRRAVLVERIA
                     ELDDTLTEKFLLGEPIELEELKAALRRGVLDNKVVPVFCGSSLRNKGVQLVLDAVVDY
                     LPSPLDIPPVQGTLVRDGSEVERIADSSEPLSALVFKIVTDPYMGRLAYIRVYSGKIK
                     QNGSIHNATKDKKERVGRLLRMYADHREDITELSAGEIGAILGLKFSFTGDTLCDPTN
                     PILLETISFPEPVISIAIEPKTKADQDKMSDSLTKLSEEDPTFKVRQDEDTGQTIISG
                     MGELHLDVLVSRLLREFKVQANVGKPRVAYHEAITKPVVGHSHRYVKQTGGHGQYAHV
                     VIDVEPLEPGSGIIFENKITGGVIPKQFIPAVEAGVRDAAESGPLAGFPMTDLKVTLV
                     DGSTHEVDSSEMAFRMAGIMALREATEKGSPVILEPIMKVEITVPEEFTGDVIAQVNA
                     RVGNILGMDERYGNAKAIHAEVPLSEMFGYATELRSATQGRGAFTMEFKHYAQVSDAY
                     MKKIL"
     gene            complement(2096..2566)
                     /gene="rpsG"
                     /locus_tag="PK791_01665"
     CDS             complement(2096..2566)
                     /gene="rpsG"
                     /locus_tag="PK791_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOH92051.1"
                     /translation="MSRRIKPEKREVKPDDRYNNIHLQMIINRIMLNGKKSLATTMVY
                     DALDIISEKTGKEGIEVFETALKNVSPLMEVRPRRIGGSTYQVPMEVAPDRRVVLAMR
                     WIITASRARSGKSYPEKLAAELMDAAENQGASVRRKEETHKMAEANRAFSHFRM"
     gene            complement(2645..3061)
                     /gene="rpsL"
                     /locus_tag="PK791_01670"
     CDS             complement(2645..3061)
                     /gene="rpsL"
                     /locus_tag="PK791_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOH92052.1"
                     /translation="MPTINQLVRHGRRTKRSKSKSPALQYTLNSEKQRRTRQPKGAPQ
                     KRGVCTIVRTMTPKKPNSALRKIARVRLSNGMEVTAYIPGEGHQLQEHSVVLVRGGRV
                     KDLPGVRYHVVRGTLDSTGVNNRKRGRSKYGTKRAE"
     gene            complement(3212..7138)
                     /locus_tag="PK791_01675"
     CDS             complement(3212..7138)
                     /locus_tag="PK791_01675"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOH92053.1"
                     /translation="MTELMPPKNYARIPVEQELPQLIEVQLESFERLKTEGLANLLNE
                     VSPIVSYGGDLRLHFPSNTPLAKEFDLKFWFEEPKNTIEECLERDLTYACPLYVSVLL
                     EGADQTEAVRQDLFLGDFPEMTQKGTFVINGTERVVVSQLIRSPGVYFEADQDRATGR
                     MMSTAKLIPDRGAWMEFETRKNDFIILKFNRKRTVPITVFLRALAALDDGIADSPLKT
                     GSDEEIMALFADVDNNPEHPYIENSFAQEPEYVTGKDIAKEALLEFYRRLRPGEPPIV
                     ENAENYLREQLFDPRHYDLELVGRYKLNQKLDLYDIIPGTHRMITKHDIVRIIRHMIQ
                     VNNGAIPKDDIDHLGNRRVKTVGELIQNKLRVGLRRMERVIKERMSIRDAGTSVLPAS
                     LVNIRPLVASLCEFFGSSQLSQFMEETNPLSELRHKRTISALGPGGLRRERAGFDVRD
                     VHFSHYGRICPIETPEGPNIGLIGRLAAFARVNEFGFIETPYRKVAQNMRGDDPNLEG
                     HLLRQNIVDPETGEIVYETETRVDKKMAKKIKELGIEVVDVRPYITDQVVFLSADAED
                     KYVIAQANAPLNEYGEFLHRNSCRYANQFSIYNYEDIDFIDVAPNQVVGISAALIPFL
                     EHDDANRALMGANMQTQAVPLLRPQVPIVSTGMEGVAVADSGQIAVAEADGEVLSVTG
                     DNILVKQSDGDLRIYPLRKYHRSNQSTCIDQRPSVVQGQRIKKGEVIIDSSSTQDGKL
                     ALGQNVVIAFIPWEGFNFEDAILISEKLVEDDRYTSIHIEKYEVEARDTKLGPEEITR
                     EIPNVGDDTIRDLDETGIIRIGAEVGPNDILVGKITPKGEKELTPEERLLRAIFGEKS
                     RDVKDTSLRMPHGERGIVVDVKVFTREENTDLSAGVDKMVRVSVAQRRKITAGDKMAG
                     RHGNKGCVSMVVPVEDMPFLEDGTPVDIILNPLGIPGRMNIGQVLEAELGWAASRLGF
                     RAITPVFDGANEAEIAAELARAWMIDEAWRAVAEKGWAWIQTFEHDPEMILDDEEVRL
                     LYLENILADKDYDIFAIKENEVLARRATLNEWLISEGFDPRTITAFPEDEIPVSQRDQ
                     QDHNALRACLQLWAKSLDRKIDSKLELAEMFKEATQIMLETGNPVPVLGKQLLRDGRT
                     GDLYDQPVMIGVMTMLKLHHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEV
                     WALEAYGAAYTLQEMLTVKSDDVQGRTNVYESIVKGTAIESPGIPAAFRVLVKELQSL
                     SLSVEAIMDDGEIVRFGKDDDRSQTPKVSTGLLDIGSDIPALEKIA"
BASE COUNT         1752 a         1919 c         1764 g         1860 t
ORIGIN      
        1 ctaatatttt cttcatgtac gcgtcagata cctgagcgta atgtttgaat tccatagtaa
       61 acgcgccacg tccttgtgtg gcggagcgca attcggtggc atagccgaac atttccgaca
      121 gtggcacctc agcatgaata gctttggcgt ttccataacg ttcgtccata cctaatatat
      181 tcccaacacg cgcgttgacc tgagcaatca catcgcccgt aaattcttca ggcacggtaa
      241 tttcaacctt cataatcggt tcaagaataa ccggtgagcc tttttcagtc gcttcgcgca
      301 atgccatgat acctgccatg cggaaagcca tctcgcttga gtcaacttca tgcgtactgc
      361 catccacaag cgtaaccttg agatcggtca tcgggaaacc cgccagggga ccactttcag
      421 cagcgtcgcg aacaccggct tccactgcgg gaatgaactg tttggggatg acaccccccg
      481 taattttgtt ttcaaagatg atgccactgc ccggttcaag gggttcgacg tcaataacca
      541 catgcgcgta ttgaccatgc ccgcctgttt gctttacata gcgatggcta tgcccaacaa
      601 caggtttggt gatggcttcg tgatatgcca cacggggttt gccgacattc gcctgcacct
      661 taaactcgcg caaaaggcgg cttaccagca catccaaatg taattcgccc ataccggaaa
      721 taatggtttg gccagtatct tcgtcttggc gaactttgaa ggttgggtct tcttcggata
      781 atttggtaag cgagtcggac attttgtcct ggtcggcttt ggttttgggt tcaatcgcga
      841 ttgaaatcac tggctctggg aagctgattg tttccagcag aataggatta gtgggatcac
      901 acaaggtgtc gccggtaaag ctaaacttga gtcccagtat cgcgccaatt tcgcctgcgc
      961 taagttcagt gatatcttcg cgatggtcgg cgtacatgcg cagaagtcgg ccaacacgtt
     1021 cctttttgtc tttggttgcg ttgtgaatgc tgccgttttg tttaattttg ccggagtaaa
     1081 cacggatata cgccaaacgt cccatgtagg ggtccgttac gattttaaat accagagcag
     1141 acaaaggttc gctgctatcc gcaatacgct ctacttcgct gccatcacgc accaaagtgc
     1201 cctgtacggg tgggatatcc aacggcgagg gtaaataatc aacaacagcg tccagcacca
     1261 actgcacgcc tttgttgcgc aaactgctgc cacaaaagac cggcaccact ttgttgtcca
     1321 aaacaccgcg tcgcaatgct gccttgagct cttccagctc aataggctcg cccagcaaaa
     1381 acttttcagt cagcgtatca tccagttcgg cgatgcgctc caccaaaacc gcgcgtctta
     1441 gggcgacttc ttcccgatat tcttcaggaa ctgcggtgat acgtggttca gaacccactt
     1501 tatcttcaaa ataccaggcg tcttgttcaa gcaaattaat aacgccggta aaaccagctt
     1561 caacacccag cgggatttgc atctgaaccg catttgcccc caatcgttcg cgaatgcttt
     1621 caatcgtgcg ttcgtatgaa gcgccggtac ggtccatttt attggcaaat gcgatgcgcg
     1681 gcacagcgta cttatcagcc tggcgccaaa ccgtttcgct ttgtggttca acaccttgca
     1741 ccgaatcgaa gaccaccaca ccgccatcca gcacacgcag cgaacgttgc acttcagcgg
     1801 tgaagtcgat atgaccgggg gtatcgatga gattcaggcg atagcctttc cactcagccg
     1861 taaccgcggc agaaacgatg gtaataccgc gttcgcgttc ctggggcatc cagtcggtaa
     1921 cagtagtccc atcgtccacg ttaccgatgc ggtaagtttt gccggtataa aacaaaatcc
     1981 gctcggtcgt ggttgtttta ccagcgtcaa tatgggcaat tacgccaata ttgcgatatc
     2041 gatcaagcgg aaattctgtc atttcaaact ctataagtgt taaagtacgg ccgtattaca
     2101 tacggaagtg agaaaacgcg cggttggcct cggccatctt gtgggtttcc tctttgcggc
     2161 ggacggaagc gccttgattt tcagcggcat ccatcagctc agcagcaagt ttttccggat
     2221 aagattttcc agaacgcgcg cgtgacgcgg tgataatcca gcgcatcgcc aaaacaacac
     2281 ggcggtcagg ggcaacttcc attggcacct gatacgttga accaccaata cgacgtgggc
     2341 gcacttccat gagtgggcta acgtttttca gcgcggtctc gaatacttcg atgccttctt
     2401 tgccggtttt ttcgctgatg atatcaagcg cgtcataaac catggttgta gccaggcttt
     2461 tcttgccatt aagcatgatg cgattgataa tcatttgcaa atgaatgttg ttatagcggt
     2521 cgtctggttt aacttctcgc ttttcgggct taatccttct tgacataaat gctcctcaat
     2581 ctaaacaagg cacttagtta cttgttccaa acaaacgtgc ctgtgtttgt atctttctat
     2641 gctattattc tgcgcgcttg gtaccgtatt tcgagcggcc acgttttcgg ttattgacac
     2701 cagtggagtc cagggtacca cgcacaacgt gataacgcac accgggcaag tccttcactc
     2761 tgccgccacg aaccagcaca acgctgtgct cttgcaattg atgaccttcg ccgggaatgt
     2821 atgcggtgac ttccataccg ttggaaagac gcacacgggc aatcttgcgc aaagccgagt
     2881 tcggcttctt gggggtcatt gtacgcacga tggtacacac accacgtttc tgaggcgcgc
     2941 ccttgggttg gcgggtgcgg cgttgtttct cagagttaag tgtatattgc agagcgggcg
     3001 atttgctctt gcttctcttt gttcttcgac catgtcgaac caattggtta attgttggca
     3061 ctttttcttt ctccgtacta ctaaactttt caataattaa tcattaaagg tcattttttg
     3121 gatggccttg ttttttcacc tgcaggcaag ctaaatcaat ccgccggcct gatggacgaa
     3181 agagtattct aacacgccat tcactctaac gtcaagcaat tttctctagt gcaggaatat
     3241 cgcttccgat atccagcaaa cccgttgaaa ctttgggcgt ttggcttcta tcgtcgtctt
     3301 ttccaaagcg aacaatttcg ccatcgtcca taatcgcctc aacagaaagg ctgagcgact
     3361 gcagttcctt gaccaagacg cggaaagcag ccggaatacc aggcgattca atggctgtac
     3421 ccttaacgat ggattcgtat acattggtac gaccctgaac gtcgtccgat tttaccgtca
     3481 gcatttcctg caaggtgtaa gcggcaccat atgcctcaag cgcccaaact tccatctcac
     3541 caaagcgctg gccaccaaac tgagccttac cgccaagggg ttgttgggtt accaaactgt
     3601 aaggaccagt tgaacgggca tggactttgt cttccaccag gtgatgcaac ttaagcatgg
     3661 tcatcacacc gatcatcacc ggctggtcat acaggtcgcc tgtgcgtcca tcgcgcaaaa
     3721 gctgcttacc caaaactggc actggatttc cagtttccag catgatttga gtggcttcct
     3781 tgaacatctc agccagttca agcttggaat caatcttcct gtccaggctc tttgcccaca
     3841 actgcaaaca agcacggagg gcgttatggt cttgctggtc acgctgagaa acaggtatct
     3901 catcttcagg gaaagcggta atcgtcctgg ggtcaaagcc ttcgcttatc agccactcat
     3961 tcaaggtggc gcgacgtgcc aaaacttcgt tttccttaat cgcgaagatg tcatagtctt
     4021 tatcagccag gatgttttca agataaagca agcgcacttc ttcgtcgtcc aaaatcattt
     4081 ctggatcgtg ttcaaaagtt tggatccaag cccaaccctt ttcagcaaca gcacgccagg
     4141 cttcgtcaat catccacgcc cgcgcgagtt ccgcagcgat ttcagcttcg ttggcaccgt
     4201 caaaaacagg tgtaatagcg cggaaaccta agcgactggc agcccagcca agctccgctt
     4261 ccaaaacctg accaatgttc atacggccgg ggatacccag ggggttcaaa ataatgtcca
     4321 ccggcgtacc atcttcaagg aaaggcatat cctcaacagg caccaccatt gacacacaac
     4381 ccttgttacc atggcggcca gccatcttgt caccagcggt aatcttacgg cgctgcgcca
     4441 cagacacacg caccatttta tccacgccgg cactcaggtc ggtgttttct tcgcgggtaa
     4501 agaccttcac atcaaccacg ataccacgtt caccgtgggg catgcgcagg gaagtgtcct
     4561 taacgtcgcg tgatttttca ccaaagatgg cgcgcagcaa gcgttcttct ggggtgagtt
     4621 ccttttcacc tttgggtgta atcttaccca ccaggatatc gttaggcccg acttccgcgc
     4681 cgatgcggat gatacccgtt tcgtcaaggt cgcggatagt gtcatcgccg acgttgggga
     4741 tttcgcgggt gatttcttcc ggtcccagct tggtatcgcg ggcttccact tcatactttt
     4801 cgatgtgaat acttgtgtag cggtcatcct caaccagctt ttcagaaatc aaaatagcgt
     4861 cttcaaagtt aaagccttcc cagggaatga acgcgataac cacgttttgg ccaagcgcca
     4921 gttttccgtc ctgggtggac gaagagtcga tgataacttc gcctttcttg attctttgac
     4981 cttgcacaac cgatggacgc tggtcgatac aagtggactg gtttgaacga tggtacttgc
     5041 gcaaaggata aatgcgcaaa tcgccgtcgc tttgttttac aaggatattg tcgccagtca
     5101 ccgaaagcac ttcgccgtcg gcttctgcca cagcgatctg gcccgagtca gccaccgcga
     5161 caccttccat accagtggaa acgatcggca cctgcgggcg caaaaggggc acggcctggg
     5221 tctgcatgtt ggcgcccatc agcgcgcggt tggcgtcgtc gtgttccagg aaaggaatca
     5281 gcgcggccga aatacccaca acctgattag gggcgacatc aataaagtcg atatcttcat
     5341 agttgtaaat cgagaactgg ttggcatagc ggcaagagtt acggtgcaaa aattcgccat
     5401 attcgttcaa aggcgcgttc gcctgggcga taacatactt atcttccgcg tcagccgaaa
     5461 ggaatacgac ctggtcagta atataaggac gcacgtcaac gacttcgatg ccgagttcct
     5521 tgattttctt tgccattttc ttatcaaccc gtgtttcggt ttcgtaaaca atctcaccgg
     5581 tttcggggtc aacaatgttt tgtctcagca aatgaccttc caggttcgga tcatcaccgc
     5641 gcatgttttg ggcaactttt cggtaaggcg tttcgataaa gccaaactcg ttcacgcggg
     5701 caaaggctgc caaacgacca atcagaccga tgttcgggcc ttctggcgtt tcaatggggc
     5761 agatacgtcc gtagtgcgag aagtgcacgt cgcgcacgtc aaagccggcg cgttcgcggc
     5821 gcaaaccacc gggacccaag gcagagatgg tgcgcttgtg gcgcagctct gaaagcgggt
     5881 tggtttcttc cataaattgc gatagctggc ttgaaccaaa gaactcgcac aatgaggcta
     5941 ccagggggcg aatgttcacc aggctggcag gcaacaccga tgtacccgca tcgcgaatgg
     6001 acatgcgttc cttaataacg cgttccatac ggcgcagacc cacacgcagt ttgttttgaa
     6061 tcagctcgcc aacagttttc acacggcggt tgccaagatg gtcaatgtca tccttgggaa
     6121 tggcaccgtt gttgacctga atcatgtggc gaatgatgcg cacgatgtcg tgtttggtaa
     6181 tcatgcggtg cgtgccaggg ataatatcgt acaggtccag tttttgattc agcttgtagc
     6241 gaccgaccag ttcaaggtca tagtgacgcg ggtcaaaaag ctgttcgcgc agatagtttt
     6301 ccgcgttttc aacgatgggc ggttcgcccg ggcgcaaacg gcggtaaaac tcaagcaaag
     6361 cttccttagc gatgtccttg ccggttacat attcaggctc ctgcgcgaag gagttttcaa
     6421 tatagggatg ttccgggtta ttgtccacat ccgcgaaaag cgccatgatt tcttcatcgc
     6481 tgccagtttt taatggcgaa tcggcgatgc catcgtccag tgccgccaaa gcgcgcaaaa
     6541 agacagtgat gggcacagtg cgtttgcggt tgaatttaag aataataaaa tcgtttttac
     6601 gcgtctcaaa ttccatccag gcgccacggt ctgggataag cttcgcggtc gacatcatgc
     6661 gaccggtcgc gcggtcctga tccgcttcaa aatacacacc gggcgaacgg attagctgcg
     6721 atacaaccac acgctctgtg ccattaatca caaaagtgcc cttttgggtc atttcaggga
     6781 aatcgcccag gaagagatcc tgtctgaccg cttcggtttg atcagcacct tccaaaagca
     6841 ccgaaacata aagcgggcac gcgtaggtaa ggtcgcgttc aagacattct tcaatggtgt
     6901 tcttgggttc ttcaaaccaa aatttcaggt caaactcttt cgccagcggg gtgttgctgg
     6961 gaaaatgcaa gcgtaaatca ccaccataag aaacgatggg ggatacttcg tttaataaat
     7021 tagccaaacc ttcagtctta aggcgctcaa aagattcaag ctgaacttcg atgagttgag
     7081 gcagttcctg ctcaactggg atgcgcgcat aattttttgg gggcatcaat tcggtcattc
     7141 acatctcctg tacgtgctaa aagcgtgggg gcatagccta acccacgcga aaatttacgg
     7201 ataaagcgca atttataatt ataaaacaaa ctggctcgag agtcaatcgt ttttcccagc
     7261 aaatcgtctt tttcaatgaa ctaaaaattg tacca
//
LOCUS       DAOSFC010000035         7098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_9213_length_7098_cov_5.584694, whole genome shotgun sequence.
ACCESSION   DAOSFC010000035 DAOSFC010000000
VERSION     DAOSFC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9213_length_7098_cov_5.584694"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PK791_01680"
     CDS             <1..224
                     /locus_tag="PK791_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92054.1"
                     /translation="EQSYGQAPPYGVIRYQDKSFEVPYNEVEKERFLDLINQLRTVEA
                     CADAPSRSHSTVSRCRGCGYQDICEQSLV"
     gene            289..1215
                     /locus_tag="PK791_01685"
     CDS             289..1215
                     /locus_tag="PK791_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92055.1"
                     /translation="MNFKTKEKKRGLELINNGFFDGDTGGGKFMAGKYPFVLQDGLLN
                     LYPEIRDEVLDYFKQNNIVWWGGKYPTGHVLSSQIACLNHLFKLRYEKDAVLNLLKTV
                     SDDFTDVFPIAENLEGYIQFEAVGGKINFLNEGNNTRGSSCTSVDALIYAQHRDGRRF
                     LIPIEWKYVELYGNEDKSAGRGGEIRKARYIDLITSSRYLNENTLTCCWFEPFYQLMR
                     QTLWAEQLLKHQPEGFQADDYLHLHVIPDGNTQLLDKTYPCSKKGMVATWKDCLSDPG
                     KYIVISPEHFWSKQSKDTDLYHYLRTRYWQVK"
     gene            1328..1427
                     /gene="ffs"
                     /locus_tag="PK791_01690"
     ncRNA           1328..1427
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK791_01690"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1449..2387
                     /locus_tag="PK791_01695"
     CDS             1449..2387
                     /locus_tag="PK791_01695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92056.1"
                     /translation="MKDSDKPAATTSDIHQEETPQVCSYEGSDYQEVFWDTGERRYED
                     AVEEVALKRLMPKQGKLLLELGAGAGRNTPRYAGWERIVLLDYSRTQLLQARERLGDS
                     SRYVYVTADIYKLPFVGGLFDGATMIRTLHHLSDANSALANVRRVLGEDAVFVLEFAN
                     KRNLKAIARYLLGKQKWNPFTKEQVEFVALNYNFHPKTVRKLLKENQFALKKQLTVSH
                     FRINALKRRVKHEILVALDSLFQHTGGIIQLSPSVFTKSVAVGSGQKPEGDAFFACPI
                     CDTSLPEARENQTCPGCGHLWTYEDGIYEFRINPES"
     gene            complement(2417..3739)
                     /locus_tag="PK791_01700"
     CDS             complement(2417..3739)
                     /locus_tag="PK791_01700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92057.1"
                     /translation="MEESTEALSTEPDERGTDRKSNPIKTYIERLKAFSTNARLFLVG
                     GFLFGIAGGINQLLFNFYVLSQGYTEGTLGKIVTARSLTTLLAAIPMGYLVDKIGRKR
                     AFFFSHLSFALSIVCILLFPSIPVFLTMGMVQGLAQSLSVVAQGPFLMENSEETERTY
                     LFSISSGIGTTASSVGQWFGGFLPGIFAGMLLVSAMDTKAYSAALWFVVLFALLALVP
                     ISFIKENRRDPSAKRSVFAPVTFFKRAPKKFTRLILPGLVTSIGAGMVMPFMNVFFRN
                     VYRLSDNSIGVIFAWGSLAMGIGLIIAPMIAERVGKIQTVVGTQALSIPFLVLLGFSG
                     RVELAVIAYYFRLTLMNMSSPIFSTFAMEQVEPEARAMLASLNSMAFNFGWAFSPTIS
                     GILQERHGFGPSFTITIILYTVSTSMYYFWFWHKKNTPATVEQAQKLN"
     gene            complement(3742..4803)
                     /locus_tag="PK791_01705"
     CDS             complement(3742..4803)
                     /locus_tag="PK791_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOH92058.1"
                     /translation="MSSYTDAKLHWEFGTAYDFFVSQLVLHHPERFSLRPNWAAGVRS
                     RLNPELRQTLEHAENALPMPIRFVFNLPGKTKNADAVLKELGSLAPLKRLELISGFER
                     INPTCQKILRETTPGNPWSDVEREMLLESTANADRKSSPRYLESLYAIWSDPKTFSDM
                     YLAALTAYKDNFFAEEEQRILPALKQALSYAQMRAGSLSLPLLLEDLTSGVRYSDLEQ
                     TKNIHLAPSFWISPLMFSVRPEKDRLIILYGARPDNMAIIPGDPVPDSLMRALKAVAD
                     PTRLRIMRFLAQSPQTAAQLSRSLRLRPPTVNHHLNELRIAGLVHITISQGGDRQFAT
                     RYDGIDAMHDLLTRYLHGI"
     gene            5102..6886
                     /gene="iorA"
                     /locus_tag="PK791_01710"
     CDS             5102..6886
                     /gene="iorA"
                     /locus_tag="PK791_01710"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOH92059.1"
                     /translation="MKHLLTGDEAVARGAYEAGVRFAAAYPGTPSTEILENISKYKGV
                     IKAEWAPNEKVALESVIGASVAGARALAAMKHVGLNVAADPFFTFAYMGVSGGAVVIT
                     ADEPGQFSSQNEQDNRNYAKAAMVPMFEPADSQEAKDMLADAFEISEQYDVPVLYRMT
                     TRVCHSKSLVELGERVEVPIKPYAKNIKKYVAVPANARIMKVKLQGNLKLLEDYSNNC
                     KWNRAEYGDTRIGIICSGDCYLYAREVFGDEASYLKLGFTNPLPAEMIKDFASKVDKV
                     YIIEENDPYIEDFVRALGIACDGKNIFPRNGEMLPEVIREVIYGEKIEADAELAEALI
                     GRPPTLCAGCPHRGFFFELGRKRNVMISGDIGCYTLSSGAPYHAIESTICMGASISAG
                     HGAQQIFDTIEDNKMRVVSVLGDSTFFHTGINSLMNVAYNKSNTVNVILDNRITAMTG
                     QQENPGSGMTVQGEITEQVNIEALVRTLGFENVRTINPNNLKEVKETLNWALELEGPS
                     VIITRWPCALKKMHEWDLKEFNNPFKTQYEVLEDVCIGCKACTRTGCPAIVFQVDKKK
                     SSIDPYKCVGCSVCAQVCPVDAIQLKGA"
     gene            6889..>7098
                     /locus_tag="PK791_01715"
     CDS             6889..>7098
                     /locus_tag="PK791_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH92060.1"
                     /translation="MTKNILLTGVGGQGTILASRLLTLGLQDNGYDVKMSEVHGMSQR
                     EGPVLTHVRYGKKVWSPVVSRKKGDI"
BASE COUNT         1944 a         1585 c         1795 g         1774 t
ORIGIN      
        1 tggagcaaag ttatgggcaa gcaccgcctt atggggtcat tcgttatcag gataagagtt
       61 ttgaagtacc ctacaacgag gttgaaaagg aaagattttt ggatttgatt aatcagctgc
      121 gtacagttga agcttgcgct gacgcgccca gccgttcaca tagcactgtg tcgcgttgtc
      181 gtggttgtgg ttatcaggat atttgcgagc agagtcttgt ttaagggacg ataatgcgat
      241 ttctagaagg gtaaatagac gttttaagtg acaaatggag gattaagaat gaatttcaaa
      301 actaaagaga aaaaacgtgg cttggaatta attaataatg gtttctttga cggtgatact
      361 ggtggcggaa agtttatggc tgggaaatat cctttcgtgc ttcaagacgg tctattaaat
      421 ttgtatcctg aaatcaggga tgaagtgctt gactatttta agcaaaataa catcgtttgg
      481 tggggtggca agtatccaac cggtcatgta ttatcttcgc aaatagcttg tttgaatcat
      541 ttgttcaaac ttaggtatga aaaagacgcc gttttgaatc tgctaaagac cgtctcggac
      601 gattttacag atgttttccc tatcgcggag aaccttgagg gatatattca gtttgaggct
      661 gtaggtggaa agattaactt cttaaacgaa ggtaataaca caagaggaag cagctgtact
      721 tctgtcgatg ccttgattta cgcacaacat cgtgatggtc gaagattctt aatcccgatt
      781 gagtggaaat atgttgagtt atatgggaac gaagacaaat cagcaggaag aggaggagaa
      841 atacgaaaag cccgctatat tgatttaatt acaagtagcc ggtatttgaa tgagaataca
      901 ctcacttgct gttggtttga acctttctat caattaatgc gtcaaacgct ttgggcggaa
      961 caactattaa aacatcaacc agaaggattc caagctgatg actatttgca tttacatgtt
     1021 attcctgatg gcaatacgca attactggat aaaacgtatc cctgcagcaa aaaagggatg
     1081 gtggcaacct ggaaagattg tttgagcgac cctggcaaat atatcgtcat ttctccggaa
     1141 catttttgga gtaagcagtc taaagatacg gacttatacc attatttaag aacacggtat
     1201 tggcaagtaa aatgaaagtg gctaagaaaa tcgtaaaacg ggcaaaatta tgcgacgcca
     1261 ctcttcagat aaaaaatgcc acgaagaatt gacacccgcc ccggaaacag gtatccttat
     1321 tcccgttggg tgggaccggt gcggcagagc cctgcgaacc ccgtcaggtc cgagaggaag
     1381 cagcggtaag gaggtttctc tgggtgttgc cggcaaacct gcccaacatt tctttaacga
     1441 ggattgacat gaaagacagt gataaaccag ctgctaccac aagcgatatt catcaggaag
     1501 aaacccccca ggtctgttcc tacgaaggct ctgattacca ggaagtattt tgggataccg
     1561 gcgagcgtcg ctatgaagac gcggttgaag aagtcgcgct aaaacggctg atgcccaagc
     1621 aaggcaagct tttgctggaa cttggcgcgg gtgccgggcg caatacgccc cgttatgccg
     1681 gctgggaacg cattgtgctc ttggattact cccgcacaca actcttgcag gcgcgcgaac
     1741 gcctgggtga ttcgtcgcgt tatgtctatg tcaccgcgga tatttacaag ctgccatttg
     1801 tcggcggtct ttttgacggt gccacgatga tccgcaccct gcatcatctt tccgatgcta
     1861 acagcgccct tgccaacgtg cgccgggtgc ttggtgagga tgctgtgttt gttcttgaat
     1921 ttgccaacaa gcgcaatctc aaagccatcg cgcgctatct cctgggtaaa caaaaatgga
     1981 accctttcac caaagaacag gtggaatttg tcgcgctaaa ttacaatttt catcctaaaa
     2041 ccgttcgcaa attgcttaaa gagaaccagt tcgcgcttaa aaaacagctc accgtatccc
     2101 attttcgcat caacgcgttg aaacgacggg tcaaacacga gattttggtt gcgttagatt
     2161 ccctgttcca gcatacgggg ggcatcattc agcttagccc gagcgtgttc accaagtctg
     2221 ttgctgtcgg atctggccaa aaacctgaag gcgatgcctt tttcgcttgc ccgatttgtg
     2281 ataccagctt gcctgaggcg cgcgaaaatc aaacctgccc gggttgtggg catctttgga
     2341 cttatgaaga cggtatctac gagtttagga ttaacccgga gagctaaagc atttcgtttg
     2401 gtgtaatctc aatcaattaa ttcagctttt gtgcctgctc aaccgtggca ggtgtattct
     2461 tcttgtgcca aaaccagaag taatacattg aggttgaaac agtataaagg atgatagtaa
     2521 ttgtgaatga tgggccaaaa ccatgtcgtt cctgcaagat accactgatt gtcgggctaa
     2581 atgcccagcc aaagttgaag gccatactgt taaggctggc taacatggcg cgcgcttctg
     2641 gctcgacttg ttccatggca aaggtggaaa aaatagggct gctcatattc atcagcgtca
     2701 atcggaaata atacgcgatt acagctaatt caaccctgcc ggaaaaaccg agtaaaacca
     2761 aaaaaggaat ggataaagcc tgggtgccta ctacggtttg aatcttgccc acacgttcgg
     2821 caatcattgg cgcgataatc aaaccgatac ccatcgccag actgccccag gcaaaaataa
     2881 caccgatgga attatccgaa aggcggtaaa cgtttcggaa aaagacattc ataaagggca
     2941 taaccatgcc agcgccgatt gaggtaacca agccgggtaa aatgagcctg gtgaattttt
     3001 tcggtgcgcg tttaaagaag gtaacaggag caaacacaga gcgcttggcg cttggatcac
     3061 gacggttttc ctttataaaa cttattggta caagcgctaa aagcgcgaac aggactacaa
     3121 accacaaagc ggcactataa gctttggtgt ccatggcgct taccaggagc atccccgcga
     3181 agataccggg tagaaagcca ccaaaccatt gtcctaccga gctggctgtc gtaccgatgc
     3241 ccgaagagat gctgaacaga taggtgcgtt cggtttcctc gctgttttcc atcaggaatg
     3301 gcccctgcgc gacaacggat aaggattggg caaggccttg caccataccc attgtcagga
     3361 atacaggtat ggatgggaac aaaaggatgc acacaatgct tagcgcgaag gaaagatggc
     3421 taaagaaaaa ggctcgtttg cgccctattt tgtcaacaag atagcccatc gggatggcgg
     3481 ccagcaaagt tgtcaggctg cgcgcggtaa cgatttttcc cagtgtccct tcagtataac
     3541 cttgactcag aacgtaaaaa ttgaagagca gttggttgat accaccagcg ataccaaaca
     3601 gaaaaccgcc gactaaaaac aagcgcgcat tagtgctaaa ggcttttagt ctttcaatat
     3661 aagttttgat gggattgctt tttctgtctg taccgcgttc atcgggctcg gttgacaggg
     3721 cttcggtgct ttcttccata tttagattcc atgcaaatat cgcgtcagca ggtcgtgcat
     3781 ggcgtcaatg ccatcatatc gtgtagcaaa ctggcggtca ccgccctggc ttatcgtaat
     3841 gtgcaccagc ccggcaattc gcaactcgtt caggtgatgg tttactgttg gcgggcgcaa
     3901 tcgtaaagag cgcgaaagtt gcgctgcggt ttgtgggctt tgtgccaaga aacgcataat
     3961 gcgcaagcgg gtagggtctg ccacggcttt gagcgcgcgc ataagactgt ctggtacggg
     4021 gtcgccgggg ataattgcca tgttatccgg acgggcacca tagagaataa ttaaacggtc
     4081 tttttccgga cggactgaga acatcaaggg cgaaatccaa aaagagggtg ccaggtggat
     4141 gtttttggtt tgttccaggt cagaatagcg cacgcctgag gtcaggtctt caagcagtaa
     4201 aggcaaagaa aggctgcccg cgcgcatttg cgcgtaagaa agcgcttgct tcagcgctgg
     4261 cagaatgcgt tgttcttctt cagcgaaaaa attgtcctta taggcggtta atgcagcgag
     4321 atacatatcc gagaaagttt tagggtctga ccaaatggca taaaggcttt ctaagtaccg
     4381 tgggctgctc ttgcggtctg catttgctgt gctttcaaga agcatctcac gttcgacatc
     4441 ggaccatgga ttgccaggtg tagtttcacg caaaattttt tggcaggtcg ggttgatgcg
     4501 ttcgaaacca gaaataagtt caagacgttt taggggcgcc aggcttccaa gctctttgag
     4561 caccgcatca gcgtttttgg tttttccagg caggttgaaa acaaagcgga tgggcatggg
     4621 taatgcgttt tcagcgtgtt caagtgtttg ccttaattcc ggatttaaac gggaacgtac
     4681 acctgctgcc cagtttggtc gcaagctaaa gcgttcaggg tgatgaagca ctaattggct
     4741 tacgaagaaa tcataggcgg taccaaattc ccagtgaagt tttgcgtccg tataagaact
     4801 cattgtgtgt aatccttaaa atatatttta ttagcagagg gttagacgac cgtctaagct
     4861 taatttgctt agttatacca ttatctaaca atggcgtcaa ttgttacatt cgtcaaatat
     4921 tcgattacaa gctaaccttt cattttatca gaaaaatcgc agtatggaaa atttaatcaa
     4981 gtaaaaaatt tttgtgaagc agttgaataa aaacaaaaaa ataaggtcct aatacccggt
     5041 atttgcgtta caatagtaat gttggcgtgt gccaagcaaa aaactatcca aggaggccat
     5101 tttgaaacat ctcttaactg gagacgaagc tgttgctcgt ggtgcctacg aagctggcgt
     5161 gcgttttgct gccgcttatc cgggtacacc cagcactgaa attctcgaaa atatctccaa
     5221 atataaaggc gtcattaaag ctgaatgggc gcctaatgaa aaagtagccc tcgagtcggt
     5281 tattggcgcg tctgtcgcgg gtgcgcgagc cttggcggct atgaaacacg ttggcttaaa
     5341 cgttgccgcg gacccgttct tcacgttcgc ttatatgggc gtgagcggtg gcgcggtggt
     5401 tatcacggcc gatgagcctg gccagttctc ttcgcagaac gagcaggaca accgcaacta
     5461 tgccaaagct gccatggtgc cgatgtttga acccgctgac agtcaggaag ccaaagatat
     5521 gctcgccgat gcttttgaaa taagcgaaca atatgatgtg cccgtgcttt accgcatgac
     5581 cacacgtgtg tgccattcca aaagtctggt tgagttaggc gaacgggttg aagtgcccat
     5641 caagccctac gcgaagaata tcaaaaagta tgttgctgtg cctgccaacg cgcgcatcat
     5701 gaaggtgaaa ctgcaaggca atttgaagtt gttggaagat tattcaaata actgcaaatg
     5761 gaaccgtgcc gaatatggcg acaccaggat tggcatcatc tgttccggcg attgctatct
     5821 ttacgcccgc gaagtttttg gcgatgaagc ctcctatctc aaacttggtt tcacaaaccc
     5881 cttgccagca gagatgatca aagactttgc ctcgaaagtt gacaaagttt acatcattga
     5941 agaaaacgac ccctacatcg aggactttgt acgcgctttg ggcatcgcct gtgacggtaa
     6001 aaacatcttc ccgcgcaacg gcgaaatgct gccggaagtc attcgcgaag tcatctatgg
     6061 cgaaaaaatc gaagctgacg cagaactggc cgaagcgctt atcggtcgtc cgcccacact
     6121 ttgcgccgga tgtccgcatc gtggtttctt ctttgagctg ggtcgcaagc gcaatgtgat
     6181 gatttctggc gatattggct gttacacctt atcatcgggc gcgccttatc atgccattga
     6241 aagcactatc tgcatgggtg ccagcatcag cgctggccat ggcgcgcagc aaatctttga
     6301 cacgattgaa gacaacaaaa tgcgcgtcgt ttcggttctg ggcgacagca ctttcttcca
     6361 taccggtatc aactccctga tgaacgtggc ctacaacaag agcaacacgg taaacgtcat
     6421 tctcgacaac cgcatcaccg ccatgaccgg tcagcaggaa aaccctggca gtggcatgac
     6481 cgtgcaggga gaaatcaccg aacaggtgaa tatcgaagcg ctggtgagaa cactgggctt
     6541 tgaaaacgtg cgcactatca acccgaacaa ccttaaagaa gttaaagaaa cgcttaactg
     6601 ggcgttggaa cttgaaggtc cgtcggttat catcacccgc tggccttgcg cgctcaagaa
     6661 aatgcacgaa tgggatctga aagagttcaa taaccccttc aaaacgcaat acgaagtgct
     6721 cgaagatgtc tgtatcggct gtaaggcctg tacccgcacc ggctgtccgg cgattgtctt
     6781 ccaggtggat aagaaaaagt ccagcattga cccctacaaa tgcgtgggat gcagcgtttg
     6841 cgcgcaggtc tgtcctgtgg atgccattca actgaaagga gcctaagcat gactaaaaat
     6901 attctactta ccggtgttgg tggccaaggc accattttag ccagccgtct tttgaccctt
     6961 ggtttgcaag ataatggcta tgacgtgaaa atgagcgaag tgcatggcat gtcccagcgc
     7021 gaaggacccg tgttgaccca cgtgcgctat ggtaaaaaag tctggtcgcc ggttgtatcc
     7081 aggaaaaaag gcgatatt
//
LOCUS       DAOSFC010000036         7074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_9253_length_7074_cov_4.413877, whole genome shotgun sequence.
ACCESSION   DAOSFC010000036 DAOSFC010000000
VERSION     DAOSFC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9253_length_7074_cov_4.413877"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PK791_01720"
     CDS             complement(<1..1134)
                     /locus_tag="PK791_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOH92061.1"
                     /translation="MKRLVSKVVSLLLLAALLVSPVVAAAPERVAPPTDEFGLTAEDE
                     LWLETARMADSFTVQLKEPSLATYEGSNALFAMPARTERGKLDFEAPETIAYLDYINS
                     KLDAFISDAETVLGRELEVLYRYDVVLNGFSAKMSLEEAAQLRQLPEVLEVYPDEIWQ
                     IDTDVSPAFIGIDQIWNGNAVPGDAGAKGAGTIVGIIDTGINMTHPSFQQTTPLDPYV
                     YPAPAGGFKGLCKTDPAGHVCNNKLIGVWDMLNNNNGHDQLDHGSHTAGTAAGNRVQI
                     NYNGASVVISGMAPNAQVLSYKVCSSSGCPSSASTAAVNQAIIDGADALNFSIGPTSG
                     PARSPWMDTTEKAFLEAFKVGVSTATSAGNSGPGASTIYKTPPW"
     gene            1552..3165
                     /locus_tag="PK791_01725"
     CDS             1552..3165
                     /locus_tag="PK791_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH92062.1"
                     /translation="MEKKRLWWKDGIIYQIYPRSFKDTNADGIGDLAGIIDSLDYIKD
                     LGVDAIWLSPIYPSPDKDFGYDVADYLTIDPKYGNLQDFDHLIAEAHKRDLHIIMDLV
                     LNHTSDQHRWFVESRKSPDNPYHDWYLWRDPAPDGGPPNNWLSHFGGPGWEYDRYLNK
                     YYFHMFEKYQPDLNWRNPEVRAMVMKIFRYWLDKGVDGFRLDVFNAYYKDADLRDNPR
                     KKGLARRPFEAYEHIYDKSQPEMMDVVKEIRSILDEYPERYVVGETFLEGSTYARTFV
                     GKDRFHAAFDFNFASRPWNAKAFGTAIQYWDALHGDEAWPNYFFNNHDVPRSATRYTN
                     NEDDSRLKLLATMLLTVRGTPYIYYGEEIGMRDIKVARKDIQDPPGKRYWPFYKGRDG
                     CRSPMQWNATVNAGFGTGKSWLPVHPNHTVRNVANQQSSPASLLNFYKALIQVRRKHP
                     SLHAGDIQLIPVDNQYLLVYLRRLPEETSLVILNFSRDIQQFELPIKEVNEWDMVFNG
                     NDSITSHMSETSVSLPAYGMAILVSRLAL"
     gene            3229..5502
                     /locus_tag="PK791_01730"
     CDS             3229..5502
                     /locus_tag="PK791_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92063.1"
                     /translation="MPFVVGHVQPDTPRTDLPLERFLPPWHDGMASRWLNNYAAPGSW
                     VLDPFGQSPFSVLEIAHAGYRVLVTANNPVSAFIIKVLATAPTLDEMQAALTLLGKSK
                     SLSGQSLIEHIQSQYLLPCPVPDCASHADNSKSKSFEVERYIWHENRKQPSQATGKCK
                     VCGHTTELELTDEHHKSLPPLPPVAMSRALALEKIAPPTDPLRAVMEDVVSYYTDRSL
                     NLLLSILTQISSQEFSSRQKMLMQALFLSAADRGNQLWAYPMGRNRPRQLLCPPVYQE
                     FNLWHSLENACHLWASQEKAITLRYYPEKLPQSGGICLFEGRLRELDPKPDPRQIETI
                     YANLPRRNQAWWNLSGLWSGWLWGKEGVKTLRNSLLKQRYDWTWHATALEKVLARLKY
                     LVRPAAPILLQVSELDPLFLLASVKAAQSAGFSLRTAACSGGVNILQTVWKFSPSQLT
                     QYSINKVRDAAREIATDFLSNWGEPSGYMRLFTQMILGLQTRGLLFNQDATQANPPIN
                     KTQEDLDPVFTDPSLFHRFSPGVSTDTGTYWLASPPEYFSTLADNVEGIIRQEFLHSP
                     KLSALDIAKAINHKHPGMMTPEHELVQTILRSYATRNEQDDQACWVLNENEQFTTRAQ
                     DIKSLREIIEKIARKLAIKRQISEEAIYFLDEHDHETYTFFVSASAMISETIQHHRGL
                     SGKKLIVLPGSRANLVSYKLRRDPNLQAMMQDDWHFVKFRLLRNIYENPLLTRELFAY
                     QILGDPPEYRVSQLALF"
     gene            complement(5520..>7074)
                     /locus_tag="PK791_01735"
     CDS             complement(5520..>7074)
                     /locus_tag="PK791_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOH92064.1"
                     /translation="GSAIKPFTFLAAFEKDWTPATLLWDVPTRFRPSLDPNDTNPDYE
                     PRNYDGRFRGPVTVRSALANSYNIPVVKALEYVHIYDNPETPEADGFINLARRLGITS
                     LTRPDYGLSLTLGGGEVSLMELTGAFQVLANEGRKLEPVAITRVVNHTGDVVFEHEPS
                     QGAQIVRAAHAYLLSSILSDNNARAPMFGTNSPLNLPFSAAVKTGTTNDFRDNWTIGY
                     TPELLIGVWVGNADYTPMVNTTGVDGAAPIWNRLMQTGINQLYGGIASNFARPNDVVE
                     RVICSVSGTEPSEYCPSHVTEIFASDQMPLPKSDDLWVRAQIDAWTNLQASPSCSEFR
                     KEALAINVTDKSAIEWLETEPGREWARNAGFPEPIVVLPERECRADDPRPIINLVGIY
                     DGGTVTENPLKIAGVIDATANFKQFIIHWGVGEDPEEWHRVTDDWVLEPIRSESLIAE
                     WDLSEIEETLITIRVTMHSTMNTEVQKFYRLRLEIPTPTPTPTLTPTETPMPTETPAP
                     PQEPTIVSP"
BASE COUNT         1816 a         1904 c         1624 g         1730 t
ORIGIN      
        1 ccatggtgga gtcttgtaaa tggtggaagc accaggacca gagttacctg ctgaagtagc
       61 tgttgaaacg ccaactttga aggcttcaag gaaagctttt tcggttgtat ccatccatgg
      121 gctacgcgca ggaccactgg ttgggccaat ggagaagtta agcgcatcgg cgccatcgat
      181 aatagcttgg ttgaccgcgg cggttgaagc actggaagga catccacttg agctacaaac
      241 tttgtaggaa agaacctgag catttggtgc cataccggaa atgacaacag aagcaccatt
      301 gtaattgatt tgaactctgt taccggcggc agtacccgcg gtatgagaac catggtcgag
      361 ttgatcatgt ccattattgt tgttgagcat gtcccaaaca ccaatcagtt tgttgttaca
      421 aacatgacca gcaggatcag tcttacaaag acctttgaag ccaccagcag gtgcgggata
      481 cacgtaggga tccaagggcg tggtttgctg gaagctggga tgggtcatgt tgataccggt
      541 gtcgataata ccgacgatgg tgcctgcacc cttcgcgccg gcatcaccag gaacagcgtt
      601 cccattccag atttggtcaa taccgataaa tgcagggcta acgtcggtat caatttgcca
      661 gatttcatca ggataaactt caagaacttc aggcaactgg cgaagctgag cagcttcttc
      721 gagactcatc ttagctgaaa aaccgttgag aacgacatcg tagcgataga gcacttccag
      781 ttcacggcca agcacagttt cagcatcgct gatgaatgca tccaatttgc tgttgatgta
      841 atcaaggtag gcgattgttt caggtgcctc gaaatccaac ttgccacgct cagtacgtgc
      901 gggcatggcg aacagcgcat tgctgccttc ataggtggcg agtgacggct ctttaagttg
      961 gacagtaaag ctgtccgcca tgcgagctgt ttcaagccac aattcatctt cggcggttaa
     1021 gccgaactca tcggttggtg gcgctacgcg ctcaggagcg gcagcaacca ctgggctcac
     1081 caacaaagcg gcgagcagta gtaaactaac tactttggat actaatcttt tcatatttgt
     1141 tactcctttt aaatatgtaa ctaccgtatc aatgatacct gatacttcta ataattgata
     1201 taaaccattt gaagctctta ttttccggtt ttcagaaagg ttttttaagc ccatttggtt
     1261 ttaatcatac cataagctga ttaatttcac tcaagttcgt aatatttaat cgattttatg
     1321 caacctccat ttctttaaaa gcaaaactgt cgaaatactt tttccaatat ttatattgca
     1381 agcattgtgc caaaatgaag gcttacatga cagtttatga cgcagctttt tcttaatcaa
     1441 accctacaca gagaccgacc aaaaacaaac tccaaagcct tcggttttta gattgagaat
     1501 tgcacacaat aacgtataat tagcttaatc gcatttggag gtgatattcc catggaaaag
     1561 aaacgcttat ggtggaaaga tggcattatc taccaaattt atccccgctc attcaaagat
     1621 acgaacgccg atggtattgg cgaccttgct ggcatcattg attctcttga ctatatcaag
     1681 gatctcggtg ttgatgccat ttggctgtcg cctatttacc catctcccga taaagatttt
     1741 ggctacgatg tcgccgatta cctgactatt gaccccaaat acggcaatct gcaagacttc
     1801 gaccacttaa tcgccgaagc ccacaaacgc gatttgcata tcatcatgga cctggtgctc
     1861 aaccacactt cagaccaaca ccgctggttt gttgaatccc gcaaatcgcc agataacccc
     1921 taccatgact ggtacctttg gcgcgacccg gcccctgatg gcggcccacc caataactgg
     1981 ctctcccact ttggcggacc aggctgggaa tacgaccgct accttaataa gtactacttc
     2041 cacatgttcg agaagtacca acccgaccta aactggcgca atcctgaagt ccgcgccatg
     2101 gtcatgaaga ttttcagata ctggctcgac aaaggcgtgg atggtttccg ccttgatgtc
     2161 ttcaacgctt actacaaaga cgctgacctg cgcgacaacc cccgcaaaaa agggcttgcc
     2221 cgccggcctt ttgaagccta cgagcacatt tacgacaagt ctcaacccga aatgatggat
     2281 gtggtcaagg aaattcgctc cattctggat gaatatcccg aacgctacgt ggttggcgaa
     2341 acatttttgg aagggtcaac ttacgcgcgc acttttgttg gcaaggaccg cttccatgcc
     2401 gctttcgact tcaactttgc atccagaccc tggaacgcca aagctttcgg caccgccatt
     2461 caatactggg acgcgctcca tggcgatgag gcctggccaa attacttctt taacaatcac
     2521 gacgtgcccc gctctgcaac ccgttacacc aacaacgaag atgacagtcg tttgaagctc
     2581 ctggcaacca tgctgctcac cgtcagaggc acgccctata tatattatgg tgaagaaatc
     2641 ggtatgcgcg atatcaaagt cgcccgaaaa gacatccagg acccacctgg caagcgttac
     2701 tggccctttt acaagggtcg cgatggttgc cgttcgccaa tgcaatggaa cgctacggtc
     2761 aacgccggct ttggtaccgg caaatcctgg ctgcccgtgc acccgaatca cacggtgcgc
     2821 aatgttgcca atcagcaatc cagcccagca tcgctcttaa atttttacaa agcactcatt
     2881 caagtccgtc gcaagcaccc cagcctgcac gcgggcgata tccagctcat tccagttgac
     2941 aaccaatacc tgctggttta tctgcgtcgc ttgcccgaag aaacaagttt ggtcattctc
     3001 aacttttcaa gggatattca acagtttgag ctacctatca aggaagtcaa tgaatgggat
     3061 atggtattca acggcaacga ctccattacc agccatatga gcgaaacaag tgtgagcctg
     3121 cccgcttatg gcatggcaat tttggtctcg cggctggcac tttaaagaca gaagacgttt
     3181 gagttaggtc atatgtacaa cccacgaacc cattggagag ctttcgaaat gcccttcgtt
     3241 gtgggtcatg tacaaccgga tacaccccga accgatttgc cactggaacg ttttttacca
     3301 ccgtggcatg atggcatggc aagtcgctgg ctgaataatt atgccgcgcc cggttcgtgg
     3361 gttctggacc catttggtca gtcgcctttt tccgtactgg aaattgccca cgctggctac
     3421 cgcgtgctcg ttaccgccaa taatcctgtt tcagctttta tcatcaaagt actcgccacc
     3481 gccccaacac tggatgaaat gcaagccgcc ctgactttac tcggtaaaag caaatctctg
     3541 agcggacaaa gcctcattga acacattcag agtcaatatc ttttgccttg tccggtgccc
     3601 gattgcgcca gtcacgctga caatagcaag tctaaatcct ttgaagttga acgctatatt
     3661 tggcacgaaa accgaaaaca acccagccag gcgacaggca aatgcaaggt gtgcggtcac
     3721 acaacagaac ttgagctaac agatgagcac cataaaagcc tgcccccgct tccacccgtg
     3781 gcgatgagtc gtgccttggc gcttgaaaaa atcgccccgc caactgaccc actccgcgca
     3841 gtgatggaag atgtcgtcag ttattacacc gaccgctcgc tcaatttgct tttgagcatt
     3901 ctcactcaga tttcttcgca ggaattttcg agtcgtcaaa aaatgctgat gcaagcgctc
     3961 tttctcagtg ccgcagaccg tggcaaccag ctttgggcat accctatggg acgcaaccgt
     4021 ccccgccagc tcttgtgccc gcctgtttat caggaattca atctttggca cagtctggaa
     4081 aacgcctgtc atctttgggc aagtcaggaa aaagccatca cattacgcta ttatccggaa
     4141 aaacttcccc aatccggtgg tatctgcctt tttgaagggc gccttagaga acttgaccca
     4201 aaacctgacc ccaggcagat tgagaccatc tacgccaatc taccacgccg caatcaagcc
     4261 tggtggaatc tgtctggttt gtggagtggc tggctttggg gcaaagaagg cgttaaaacc
     4321 ctgcgcaaca gtttgctcaa gcaacgctat gactggacct ggcacgcgac cgccctggaa
     4381 aaagtcctgg cacggttaaa gtatctcgta cgcccggcag cccccattct gctccaggtt
     4441 tccgaacttg acccgctctt tttgctggca tcagttaaag ccgcgcaaag tgccggattc
     4501 agcctgcgca cggcagcttg ttcgggtggc gtgaatatat tacaaacggt ttggaagttt
     4561 tcgcccagcc agcttacaca atattcgata aacaaagttc gcgatgccgc ccgcgaaatt
     4621 gccacagact tcctaagtaa ctggggcgag ccttctggct atatgcggct tttcacccaa
     4681 atgattcttg gcttacagac acggggtttg ctcttcaacc aggatgcgac acaggcaaac
     4741 ccaccaatca acaaaaccca ggaagattta gaccccgttt tcaccgaccc cagcttattt
     4801 caccgtttca gcccgggcgt atcaaccgat acgggcacat actggctggc aagtccacct
     4861 gaatatttca gtactctggc ggataacgtc gaaggaatca ttcgtcagga atttctccat
     4921 tcacccaaac tcagcgcgct tgatattgca aaagcaatca atcacaaaca cccaggcatg
     4981 atgacacctg aacacgaact tgtgcaaacg attttgcgtt cttatgctac acgcaacgaa
     5041 caggatgacc aggcctgctg ggttttgaat gaaaacgaac agtttacaac gcgcgcgcag
     5101 gacatcaaat cactccgtga aattattgaa aaaattgccc gcaaactggc cataaaacgc
     5161 caaatcagcg aagaagctat ctactttctt gatgaacacg accacgaaac ttataccttt
     5221 ttcgttagtg ccagcgcgat gatatccgaa acaattcaac atcatcgcgg tttaagtgga
     5281 aagaaactga ttgttctgcc cggcagccgc gccaacctgg tctcttacaa actgcgtcgc
     5341 gacccaaatc tgcaagccat gatgcaagat gactggcatt tcgtcaaatt ccgtctcctg
     5401 cgcaatattt acgagaaccc gcttctcacc cgcgaattat tcgcctatca aatactcggc
     5461 gacccgcccg aataccgcgt gtcgcaactc gctctgttct aagccagtgc tctgctcacc
     5521 tatggtgaaa caatcgttgg ttcctgtggc ggagcaggtg tttccgttgg catcggcgtc
     5581 tctgttggcg tgagcgtcgg ggtcggcgtt ggcgtgggga tttccaggcg caaacgataa
     5641 aacttctgca cttcagtgtt catcgtgctg tgcatggtca cgcgaatggt aatcaaagtt
     5701 tcttcaatct cgcttaaatc ccactctgca atcaggcttt cactccgtat cggttccaaa
     5761 acccaatcat cagtaacccg gtgccattct tcaggatctt ctccaacccc ccagtgaatg
     5821 ataaactgct tgaagttcgc ggtcgcgtcg ataacaccag cgatctttag cggattttcg
     5881 gtcactgtgc caccgtcata aatccctacc aggttgataa taggccgtgg gtcatccgcc
     5941 cgacattcgc gctcaggcaa aacaacgata ggctcaggga aaccggcatt tcttgcccat
     6001 tcgcgtcctg gttctgtttc cagccattca atcgcggatt tgtccgttac attaatggct
     6061 aatgcttctt ttctaaactc tgagcatgat ggcgaggcct gcaaattagt ccatgcatca
     6121 atttgcgcgc gcacccaaag gtcgtcgctt ttaggcaaag gcatctggtc tgacgcaaaa
     6181 atttcggtta cgtgactcgg gcaatactcg ctgggttccg taccggaaac actgcaaata
     6241 acccgctcca cgacatcatt cggtcgcgcg aaattactgg caataccgcc ataaagctgg
     6301 ttaatacctg tttgcatcag gcggttccaa atcggtgcgg cgccatcaac accggttgtg
     6361 ttgaccatcg gtgtgtaatc ggcattaccc acccaaacgc caatcaaaag ttcgggggtg
     6421 tatccaatcg tccagttatc tctgaaatcg tttgtcgtac ccgtctttac cgcggcacta
     6481 aaggggagat tcaagggcga gttcgtgcca aacatcggcg cgcgcgcgtt gttgtcagac
     6541 aagattgaag aaagcaaata ggcatgcgcc gcacggacaa tttgcgcgcc ctggcttggc
     6601 tcatgttcaa aaacaacgtc accagtgtga ttgacgaccc tggtaattgc tacaggctca
     6661 agtttgcgtc cctcatttgc caaaacctgg aatgcaccgg tcagctccat caggctcact
     6721 tcgccaccgc ccagagtgag cgagagccca taatccggcc gggtcaggct ggtgatgccc
     6781 aagcgcctcg ctaaattaat gaagccgtcc gcttcgggag tttcgggatt gtcatatata
     6841 tgtacatact ccaaagcctt cacaaccggg atattatagg aatttgccaa agctgaacgc
     6901 actgtcaccg gacctctaaa gcgcccgtcg tagttgcggg gttcatagtc ggggttggtg
     6961 tcgttggggt caagcgaggg tctaaacctg gtaggcacat cccacaagag cgtagccggc
     7021 gtccagtctt tttcaaacgc cgccagaaaa gtgaaaggct taatcgccga accc
//
LOCUS       DAOSFC010000037         6911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_9541_length_6911_cov_4.757730, whole genome shotgun sequence.
ACCESSION   DAOSFC010000037 DAOSFC010000000
VERSION     DAOSFC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9541_length_6911_cov_4.757730"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PK791_01740"
     CDS             complement(<1..851)
                     /locus_tag="PK791_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92065.1"
                     /translation="MKQQDELLKEKEADKKNPFAAGMSQNNKDQSKRQAPRRGPSRFM
                     PGEKARDFKGSIGRLITYMGRYKWLILIILFVAAGSSVFSIFGPKTLGRATTELFEGV
                     MRMVRGDALGIDFAKIGSILSRVLLMYGFSALFGLIQGRVMAVISADIIYRLRNNLSE
                     KLHRLPLSFYDRVTQGEVLSRITNDVDTIGHTLNQSLSQIITSVTTVIGVLIMMLSIN
                     LTLTLVGLTVIPLSLLMIRFVVSRSQGYFRKQQAYLGHISSHIEEMYGAHVVLKTNNG
                     EEASIEH"
     gene            complement(848..1531)
                     /locus_tag="PK791_01745"
     CDS             complement(848..1531)
                     /locus_tag="PK791_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92066.1"
                     /translation="MHDISFKAQPGQVTAVIGSTGSGKSTLVNLLPRLFDVSKGKITL
                     DGIDIRDLTQYDLREQISYAPQRGLLFSGTVKSNLSVGKHDATEEEMRHAIEVAQVAD
                     FVLSDKDGLDMEIAQAGTNLSGGQKQRLSIARALMKDSPVYIFDDSFSALDFKTDAAL
                     RKAIKPQIADKTVFVVTQRVATARNSDQILVLDDGRLVGVGHHDDLMEDCQVYREIAQ
                     SQLSEEELA"
     gene            complement(1483..3093)
                     /locus_tag="PK791_01750"
     CDS             complement(1483..3093)
                     /locus_tag="PK791_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH92067.1"
                     /translation="MWRLRKFYYPYWANILIAALMVVVVAWSNLALPDYLSQIVNTGI
                     QKNGIEHGVPEVMRQSTLDQVLALMTPEEQAELTQAYTLVEPDSPEASQYLAKYPVLE
                     TEAVYLFNATKSKDDIAHFEALMAKPLVITQAFNLIQNEPEQASQLLGDDFPFDFARL
                     PAGMDIAQILAQMPEAQRLEMRQKIDAHLEKLEGTILRQMAISAVTDEYKAVGIDMAK
                     MQRQYILKTGAQMIGMSVLAVVSSITVSLFAARTSTALARDIRAAVFKKVESFSSAEF
                     GDFSTASLITRTSNDVMQVQMVTFMAIRMAIQAPMLGVLGVVRAMDKSPNMWWTIALA
                     VVILVLFIILIFSLVTPRFKRMQALIDRLNLIMRENLSGILVVRAFNKQGFEIKRFDQ
                     ANQDVAENMRGIGRTMSFMHPIMTLVMTGVQVLVIWVGAHQVAKSTMQVGDLMAFMQY
                     AMQILSAFLNLSMFFIIMPRAAVSADRILEVLDRENVILDPENPVALPQTIRGEIEFT
                     TLIFVIPVARKTFCMISALKPNPDRLPR"
     gene            complement(3104..3568)
                     /locus_tag="PK791_01755"
     CDS             complement(3104..3568)
                     /locus_tag="PK791_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOH92068.1"
                     /translation="MSLELSNALHAWADLATRQSMESNRQYMHERGYSFAQMNSLFFI
                     HHHDQSNINELACHLGISKAAASQMINRLVEFGLVNRLEDPQDRRIKLLTLTEEGQAL
                     IRGNRVARHAWIDAFCAYLGEKDTAPIADALETLVEHLKAYKEYLEEKKSEE"
     gene            3916..4794
                     /locus_tag="PK791_01760"
     CDS             3916..4794
                     /locus_tag="PK791_01760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOH92069.1"
                     /translation="MNKNESTAWRIIKGILIILAGLILLVGINWIGKVTSPDTPQTNK
                     NITMLFSSVGTILILYAIGRFLLPSGFVSMNLPDKYGIKHYLQGLAFGSGMILTYLIL
                     ALVLGGLSYQGLGKMTVLNIILYFLGFILQGFKEELLVRGLIQGLIARKSKLLSIILP
                     SILFGLLHLGNDNFSYLAMINTALIGLIFAFVTDLTGSLWLASAAHSIWNFLLGPFFG
                     LYVSGIPMPESVFAFSRVAGKEILSGGLYGPEAALLLTIILILFLVPLVRAYILKYKK
                     QEVEQDLALPPSDELN"
     gene            complement(4816..6300)
                     /gene="glpK"
                     /locus_tag="PK791_01765"
     CDS             complement(4816..6300)
                     /gene="glpK"
                     /locus_tag="PK791_01765"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOH92070.1"
                     /translation="MKKYIIAIDQGTTSTRCAVFDKQARLQAIKHKEHRQIMPHAGCV
                     EHDALEIWNNVLDLIPECLQEASASVKDIIAVGITNQRETTVVWDKTTGLPVYNAIVW
                     QDTRTADFMTNFEATHGAENLQKRLGLPANPYFSASKLRWILENVPGARQKAEAGTLL
                     FGTIDTWLIWKLTEGLHITDVTNASRTFLMNIKTLTWDDEMLKAFDIPCQVLPEIRPS
                     SEVYGEISHPVLKGVPIAGNLGDQHAALFGQCCFSPGEAKNTYGTGCFMLMNTGNEAI
                     FSQNGLLTTLAYQVKNQAAVYALEGSVAIAGALVQWLRDNLGIIRASEEIEELALRVD
                     DNGGLYFVPAFSGLFAPYWRSDARGVIVGMTRFSNAGHIARAALEATAYQTADVFEAM
                     RKDSGVTLSSLKVDGGMVRNRLLMQFQADILNIPVLRPMIDETTALGAAFAAGLASGF
                     WSGFDELKRTWQAREQWLPAMDARTRQGLLAGWHKAITRSYDWV"
     gene            6491..>6911
                     /locus_tag="PK791_01770"
     CDS             6491..>6911
                     /locus_tag="PK791_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH92071.1"
                     /translation="MQNNAYDIIIIGGGVVGSMIARFLSRYELKLLLIEKEADLCMGA
                     SSANTAIIHAGYDPLPGSLKAKLNVRGNAMFEQLSQELGFAFERSGDMVVAIGEEEWP
                     NLLALYEMGKQNGVPDMHLFDADEVKRREPKINPSAIG"
BASE COUNT         1728 a         1721 c         1672 g         1790 t
ORIGIN      
        1 aaatgttcga tgctggcttc ttcgccgttg ttggtcttga gcaccacatg cgcgccgtac
       61 atttcttcaa tatggctgct gatatgcccc aaataggctt gctgcttgcg gaagtagccc
      121 tgcgaacggc tgaccacaaa gcgaatcatc agcagcgaaa gcggtatcac cgtcaagccc
      181 accagggtca gggtcaggtt gatggaaagc atcatgatta agacaccgat taccgttgtg
      241 actgatgtga taatctgcga caggctttga ttgagcgtgt gtccgatggt gtcaacgtcg
      301 ttggttatgc gcgaaagcac ctcaccctgg gttacccggt cgtaaaagct gagtggcaag
      361 cggtggagtt tttcagaaag gttgttgcgc aagcggtaaa tgatatccgc cgaaatgaca
      421 gccatcacgc gcccttgaat caagccaaac aaagccgaga aaccgtacat caaaagcacg
      481 cggcttagaa tactgccgat tttggcaaaa tcaatgccca gggcatcgcc acgcaccatg
      541 cgcatgacac cttcaaagag ttcggtcgtc gcgcgcccta aagtcttagg tccgaaaatc
      601 gaaaagaccg atgaacccgc tgccacaaac aaaataatca agatgagcca tttatatctg
      661 cccatgtaag tgattaatcg ccctatgctg cccttaaagt cgcgggcttt ttcgccgggc
      721 ataaatctgc tgggaccacg ccggggcgct tgccgcttgc tttggtcttt gttgttttgc
      781 gacatacccg cggcaaaggg atttttctta tcggcttctt tttccttcag gagttcgtct
      841 tgttgtttca tgctaactcc tcctcgctta gttgagattg cgcaatttca cggtagacct
      901 ggcaatcctc catcaaatcg tcgtgatgtc ccacgccgac cagccggcca tcgtcgagca
      961 ccaaaatctg gtccgaatta cgcgcggttg ccactctttg tgtgaccaca aaaaccgttt
     1021 tatcagcaat ttgcggttta atcgccttgc gtaaagcggc gtcggtctta aagtcgagcg
     1081 ccgagaaact gtcatcaaaa atataaaccg ggctgtcttt catcagcgcg cgcgcaattg
     1141 aaagacgctg cttttgcccg ccagacaagt tggtacccgc ctgggcgatt tccatatcca
     1201 gtccgtcttt atcgctgagc acaaagtcag ccacctgagc gacttcaatc gcgtgacgca
     1261 tttcttcttc agtggcatca tgtttaccca ccgaaagatt tgatttcacc gtgccgctaa
     1321 agagcaaacc ccgttgtggc gcgtagctga tttgttcgcg caagtcgtac tgggtcagat
     1381 cgcgaatatc gatgccatcc agcgtaattt tgcccttact cacatcaaaa aggcggggca
     1441 aaaggttcac cagtgtcgat ttaccacttc ctgtggagcc aatcaccgcg gtaacctgtc
     1501 cgggttgggc tttaaagctg atatcatgca aaacgtcttc ctcgccaccg ggataacgaa
     1561 aatcaacgtc gtgaattcaa tctcgccgcg aatggtttgg ggtaaagcaa ctgggttttc
     1621 ggggtcaaga atgacgtttt cgcggtcgag tacttcaaga atgcggtccg ctgaaactgc
     1681 cgcgcggggc ataattatga agaacatcga aaggttcaaa aacgcgctta agatttgcat
     1741 tgcatactgc atgaaagcca tcaagtcgcc cacttgcatg gtcgattttg ccacctgatg
     1801 cgcgccgacc caaatcacaa gtacctgcac accagtcatc accagcgtca tgatggggtg
     1861 catgaaggac atcgtgcgtc cgatgccacg catattttca gcgacatcct gattagcctg
     1921 gtcaaagcgt tttatttcaa aaccctgctt gttgaaggcg cgcacgacca aaataccgga
     1981 gagattctca cgcataatca ggttaaggcg gtcaatcaaa gcttgcatgc gtttaaaacg
     2041 tggggtaacc aaagaaaaga tcaagatgat aaacaagacc aggatgacaa cagccagcgc
     2101 gatcgtccac cacatattcg ggcttttatc catagcacgc acaacaccca ggacacccaa
     2161 cataggggct tgaatagcca tgcgaatggc cataaaggtg accatttgca cctgcatgac
     2221 atcgtttgac gtgcgcgtaa tcagcgacgc ggtggagaaa tcgccaaatt cagcacttga
     2281 gaaactttcc accttcttga agactgccgc gcggatatcg cgtgccaggg ctgtggaggt
     2341 gcgcgccgcg aataagctca ctgtaatcga gctgacaacc gccaaaacag acataccgat
     2401 catttgcgcg ccggttttta ggatgtattg ccgttgcatt tttgccatgt caatgccaac
     2461 ggctttgtat tcgtcggtaa ctgccgatat cgccatttgc ctgagaatcg tcccttcaag
     2521 tttttcaagg tgcgcgtcga ttttctgacg catttccaga cgctgggctt caggcatttg
     2581 cgccaggatt tgggcgatgt ccatgccagc tggcaatcgc gcgaaatcaa aggggaagtc
     2641 atcacccaaa agctggctgg cttgttccgg ttcgttttgg atgagattaa aagcctgtgt
     2701 gataaccagt ggtttcgcca tgagtgcttc aaagtgagct atgtcatcct tgcttttagt
     2761 cgcattgaag agatacaccg cttccgtttc aagcactggg tatttggcaa gatactgact
     2821 ggcttccggg ctatccggtt ccaccagggt ataagcctga gtgagttcag cttgttcctc
     2881 cggcgtcatc agtgccaaaa cctggtccag agtcgactga cgcatcactt ccggaacgcc
     2941 atgttcgata ccattctttt ggatgcccgt gttcacaatt tgtgatagat agtcgggcaa
     3001 ggccaggttc gaccaagcca ccacgaccac catcagggcc gcaatgagaa tgtttgccca
     3061 gtagggatag taaaattttc ttaatcgcca catagattct tactcattcc tcagatttct
     3121 tttcttctaa atattcctta taagctttca agtgttcgac caaagtctcc aaggcatcag
     3181 caatcggcgc tgtgtctttc tcgccgagat aggcacagaa cgcatcaatc caggcatgac
     3241 gcgcgacgcg gttgccgcga atcagggctt gcccttcttc ggtcagggta agcagtttta
     3301 tgcgccggtc ctggggatct tcaagcctat tcaccaggcc aaattccacc aggcggttaa
     3361 tcatttgcga tgccgcggcc ttgctgatgc ccaaatgaca ggccagttca ttaatgttgc
     3421 tttggtcatg atgatgaatg aaaaacaggc tgttcatctg cgcgaagcta taaccgcgtt
     3481 cgtgcatata ctggcggttg ctttccattg attgccttgt ggctaaatcc gcccaggcgt
     3541 gtaaagcgtt tgataattcg agagacatat tccttccttt aatagttaag cgacgtgaac
     3601 tataaagcta acttaactaa ttgtcaagcg cttttacatg ctttcccgat ggaatgcaaa
     3661 aatggttttg ttgaaagata ttttgccggg ttgatgatgg gcaagcgttt ttttaagtat
     3721 cgaaaggata tagaaccgat agaaagttta cagaaattaa gctggaattt tacataggca
     3781 tgctaaaatg gctcaatatt aaagaataat tgaattctgc tgaaaggcat tttaaacacg
     3841 gttaggacaa aaccttgcca gttgaaacaa ggtgaaagaa aaccgaacaa aactttttta
     3901 tcggaggcct atataatgaa caaaaatgaa tcgacagcgt ggcggataat taagggaatc
     3961 ttgattatcc tcgccggttt gattttatta gtcgggatta actggattgg caaagtgaca
     4021 agtccagata cccctcaaac aaacaaaaac atcactatgt tgttctcgtc tgtaggaaca
     4081 attcttatcc tttatgcaat cgggcgtttt ttattgccca gtggatttgt ttccatgaac
     4141 ctgccggata aatacgggat taaacattat ttacagggtc tggcctttgg aagtggcatg
     4201 attttgactt acttgatttt ggcccttgta ttaggtggat taagctatca gggtttgggt
     4261 aagatgactg ttctcaatat tatcctgtac tttttaggct ttatccttca gggttttaaa
     4321 gaagaacttt tagttcgtgg cttgatacaa ggacttattg ccagaaaaag caaacttctt
     4381 agtattatcc tgccatctat tttatttggt cttttgcact tgggtaacga caacttttcg
     4441 tacctggcaa tgataaatac agcgttaatc ggattaatct ttgcgtttgt aacagatttg
     4501 acgggttcct tgtggctggc ttcagcagcc cacagcatct ggaattttct cttgggcccc
     4561 ttcttcggac tttacgttag tggcatccct atgccggaaa gtgtatttgc cttctcccgt
     4621 gttgctggca aagagattct cagtggtggc ttgtacggtc cggaagcggc acttttgctt
     4681 acaattatct tgattctgtt tcttgtgcct ctcgtgcggg cttatatcct taaatataag
     4741 aagcaggaag tggaacaaga cctggcactg ccaccaagcg acgaattgaa ttaaagcaaa
     4801 aacgcaactt tataactata cccagtcgta actgcgcgta atcgctttgt gccagccggc
     4861 taacaggccc tggcgcgttc gcgcgtccat cgctggcagc cattgttccc gcgcctgcca
     4921 ggtgcgtttg agttcgtcaa agcctgacca aaaaccgctt gccagtcccg ccgcgaaagc
     4981 cgcccccaag gctgtggttt catcaatcat cgggcgcagg acgggaatgt tcaggatatc
     5041 cgcctggaac tgcatcaaaa gccggttgcg caccatgccg ccatccactt ttagcgaact
     5101 gagcgtcacg ccactgtctt tgcgcatcgc ttcgaacacg tcggctgttt gataagcggt
     5161 agcttccagc gccgcccgcg cgatatgtcc ggcattgcta aagcgggtca tgcccacgat
     5221 aacgccccgc gcgtccgacc gccagtacgg cgcgaaaagc cccgaaaaag ccggcacgaa
     5281 atataggccc ccgttgtcat caacacgcag tgccagctct tcaatctctt ctgaagcgcg
     5341 aataatgccc aaattatccc gcagccactg caccaaagca ccggcaatcg ccaccgaacc
     5401 ttccagcgcg tagaccgctg cctggttttt cacctgatag gccagcgtgg tcagcaaacc
     5461 gttttggcta aaaatggctt cgttgccggt gttcatcaac ataaaacagc ccgtaccata
     5521 ggtgtttttg gcttcgccag gggaaaaaca gcactgccca aaaagcgcgg cgtgttggtc
     5581 gcccaaattg cccgcgatgg gcacgccttt gagaaccgga tggctgattt caccgtaaac
     5641 ttcacttgaa gggcgaatct cgggcagaac ctgacagggg atatcaaagg ctttcagcat
     5701 ctcgtcatcc caggtcaaag tcttgatgtt catcaaaaaa gttctagacg cgttggtgac
     5761 gtcggtgata tgcaagcctt cggtcagctt ccaaatcagc caggtatcaa tggtgccaaa
     5821 gagcaaggtg ccggcttcgg ctttctggcg cgcgccgggc acgttttcca aaatccagcg
     5881 cagtttggac gcggagaagt atggatttgc cggtaagcca aggcgttttt gaagattttc
     5941 agcgccatgg gttgcttcaa aattggtcat gaaatcggcg gtgcgggtat cttgccagac
     6001 aatcgcgtta tagacaggca gtccggtcgt cttatcccaa acaacggtcg tttcgcgctg
     6061 attcgtgata cccaccgcga taatatcttt gacactcgcc gaagcttctt gcaaacattc
     6121 ggggattagg tcaagcacat tgttccagat ttccagggca tcatgttcca cacaaccagc
     6181 atggggcata atttggcgat gttccttgtg cttgattgcc tgcaaacggg cttgtttatc
     6241 aaaaaccgcg cagcgtgtgc tggttgttcc ctggtcaatg gcgataatat actttttcat
     6301 gatgcctcca tagtacacat cgattctaat gatgaaagaa tgtcttagtc gtggtcagga
     6361 gaccacgaaa gaactattaa tatgacacaa tcataccaag ttgtcataga attagggatg
     6421 agtccgtgcc aggatttttg ttgtcctgac agatttatcg tatcattgtt ggacacggac
     6481 aggagatttt atgcaaaaca acgcgtatga catcatcatt atcggcggtg gcgtggtggg
     6541 gagcatgatc gcgcgctttc tatcgcgcta tgagcttaaa ctcttgctta tcgagaaaga
     6601 agccgacctg tgcatgggcg catcatccgc caacaccgcc attattcacg ccggttacga
     6661 ccctttaccc ggctcactca aagccaaact gaacgtgcgc ggtaatgcta tgtttgagca
     6721 actctctcag gagctcggtt tcgcctttga acgttccggc gatatggttg ttgccatcgg
     6781 cgaagaagaa tggccaaacc ttttggcact ttatgaaatg ggcaaacaaa acggcgtgcc
     6841 cgacatgcac ctgtttgacg ctgatgaagt caaacgacgt gagcctaaaa tcaacccatc
     6901 agcgataggc g
//
LOCUS       DAOSFC010000038         6862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_9634_length_6862_cov_5.253563, whole genome shotgun sequence.
ACCESSION   DAOSFC010000038 DAOSFC010000000
VERSION     DAOSFC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9634_length_6862_cov_5.253563"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..528)
                     /locus_tag="PK791_01775"
     CDS             complement(19..528)
                     /locus_tag="PK791_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92072.1"
                     /translation="MKNKTKITILVILVGLLVFGGFAISAVKAQTPATEDTLPQPQVT
                     SRFEQRAQLRAERRALRLNQSEDCACIDLSNEECEALRLEHRLARLDERLAQGLITQE
                     HYDVIRAKIEAGEQAPYGLRDGSGLGLGSQGRGAGQGNGQGSRQGQGQGAGAGRRGQQ
                     NNGDCPYLP"
     gene            complement(653..2104)
                     /locus_tag="PK791_01780"
     CDS             complement(653..2104)
                     /locus_tag="PK791_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH92073.1"
                     /translation="MKTSLKTKLLLAFVLVALITGAVLVFVFNRFNQRQFNQFVIDKQ
                     REELISVLTNHYQEHQNWDGAQIYFSSISRSQSQGQGFGQSAGPSNQGRGRRQGQVDS
                     NHPKDSQSETGALISRRLFGLAASDATVLISSFDSPNPGEVLNAEALATSTPIIIDEK
                     TVGYLLSPNQFPGYSEVESRFVRSSNIALIIALAGAIVLAGIVAFFIARSITKPIETL
                     SKASQRIAQRELSTKVPDLGKDEIGTLARNFNEMSDHLEESDRLRKQMTADIAHDLRT
                     PLTVINGYVESMRDGDLAVTPERLGLIADETMRLSKMVDDLRFLSVADTGELNLQKQL
                     TDPNELLEHCGKLFENQAERKSITLKLELLPTAKKVLIDEDRMIQALSNLLNNAIEYS
                     PEGSQVILRLSEETAYMLLEIVDSGPGIDPANLPYIFERFHRADRSRQESETHSGLGL
                     AIAKAIITAHQGEISAQSTLGQGTRMQIRLAKY"
     gene            complement(2111..2791)
                     /locus_tag="PK791_01785"
     CDS             complement(2111..2791)
                     /locus_tag="PK791_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH92074.1"
                     /translation="MAETILIIDDQQNLRNMLKDYFSEQGYHVLTAKDGQEGLFVARY
                     ENPDLILLDIMMPRLDGYQFLQAFRKESQVPVIVLTAKTEETDTVQGLELGADDYVLK
                     PFSMRELAARVRVALRRANPSVEAEEILKAGDVVLNVTTHTVSINKEAVLLTPIEFEL
                     LSILMRSPNKVFTRADLVDRLYETGFMGLESTLNVHVRNLRAKVEKDPANPSLITTVF
                     GVGYRLNK"
     gene            complement(2883..3617)
                     /locus_tag="PK791_01790"
     CDS             complement(2883..3617)
                     /locus_tag="PK791_01790"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOH92075.1"
                     /translation="MNEKINLAREFIKSSDNIVFLGGAGVSTASGIPDFRSATGLYNR
                     AHNANYSPEYLLSYDYFKADPKGFYTYLRENLYYPDAQPNAAHYALAKLEKLGKLKAV
                     ITQNIDNFHQRAGSSNVLELHGTMEHYYCIRCHKDYDVTYVMRTKEVPLCTCGGIVRP
                     DVVLYGEMLNTDVLNASIKAITRADMFIIGGTSLVVYPAASLVRYYRGDKLLLINQDP
                     TAYDNWANLVIAGDISQVMASLVEDL"
     gene            complement(3614..4261)
                     /locus_tag="PK791_01795"
     CDS             complement(3614..4261)
                     /locus_tag="PK791_01795"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HOH92076.1"
                     /translation="MDNNTEKETEKVTQDPQKNAKLKHFAHQLNAFLIKNTPGMRVIK
                     TMIAVFICLGIEAIRQKTGTVYHSSIAAVVCMQPTVKSTAKTARERVIGTIIAGIYGF
                     LVLNFIFPNVTVDTREWYYYLVTALLSLPLMTIMIWIKTPGPIAITVIVYLIVTLKTT
                     DIPPIAYTLERVADTLIGIAVALLVNWFPPLNKLGKKMGQVEIAPAPEQDQQIKA"
     gene            4443..5018
                     /locus_tag="PK791_01800"
     CDS             4443..5018
                     /locus_tag="PK791_01800"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH92077.1"
                     /translation="MFPVAHPKTMQKARELLALNPIFLDTETTGTGANDVIIEVGIVD
                     INGDVLYESLVNPGMPIPPESSAVNEITDEMVADKPGWAQVWREVEPILRNRAIGIYN
                     ADFDMRLLKQSCAYAGVPWTLDDKQAFCVMRLYAAWYGEWNARYNGFRSQKLASAGGF
                     CGIDLPNSHHAVDDARLTAVLLRYLADYVKD"
     gene            5225..6463
                     /locus_tag="PK791_01805"
     CDS             5225..6463
                     /locus_tag="PK791_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92078.1"
                     /translation="MEKNSENKIKNLPPNLWAVSITSFLMDISSEMVINVLPLFLFSV
                     LGVKTNIIGIIEGIAEATASILKVFSGWLSDKMRSRKWLAVLGYGLSAIVKPLFYFAN
                     SWSAVAAIRWGDRVGKGIRTAPRDALVADTVKPEQRGLAFGLHRAADTAGAMLGILIA
                     ILVVWRVQATGIGLQENTFRTIVLISIIPAVLAVLALIFGTHEVKVEGVRQAPKISFK
                     GLGKNFLVFMLIVGIFDLGNSSDAFLVLRAQERGMSVLNILIMLAVFNLVYALVSTPA
                     GGLSDRIGRRKVIIGGWIIYALIYLGFALAKNVTHVFLLYVAYGVYYGMAYGTAKAMI
                     GDLVPEEMRGTAFGTYNAVLGILDFPASMIAGFLWSGVGKWAGFGASAPFYFGAIMAT
                     LAILLFVFWKPEPLSAAENG"
     gene            6550..>6862
                     /locus_tag="PK791_01810"
     CDS             6550..>6862
                     /locus_tag="PK791_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92079.1"
                     /translation="MRIGILDYRMDYYTIKRIILNKVPGFDYVPVKDLYSISRRVALR
                     FNRLFEKTLFSTFNLNNQFEDFDLNKVDVLHFSNGISYGKTPWVSQFETILPRFDYLL
                     QR"
BASE COUNT         1690 a         1416 c         1736 g         2020 t
ORIGIN      
        1 ttgcgtgtag gtaagctttt agggaaggta ggggcaatcg ccattgtttt gttgaccgcg
       61 tctgcccgcg ccagcacctt ggccttgtcc ttgtcgggaa ccctggccat taccttgacc
      121 agcgcctcgt ccctgggaac cgagtcccaa accgctaccg tcgcgtaagc cataaggtgc
      181 ttgttcgcca gcttcaatct tagcgcgaat gacatcgtag tgttcctggg tgattaagcc
      241 ttgagccaag cgttcgtcca ggcgagccaa gcgatgttcc agacgcagag cttcacattc
      301 ttcgttgctc aagtcaatgc aggcgcaatc ttctgattgg ttcaaacgca gcgctcgtct
      361 ttcagcacgc agttgcgcgc gttgttcgaa tcgactggtg acctggggtt ggggtagagt
      421 atcttcagtg gcaggggttt gggctttgac tgcgctaatg gcaaatccac caaaaaccaa
      481 aagaccaacg aggatgacta aaattgttat ttttgttttg tttttcattc ttatctcttt
      541 ctgtaagtcg tggtttagta tcgtcatatc tgttttatca acttacatcc ttatgatagc
      601 aagaacttat ttagaactga tttagaagaa aaaagatttt ttaccaaatg tactagtact
      661 tggctaatct aatctgcatc ctggtgcctt gtcctaatgt tgattgagcg gatatctcac
      721 cttggtgagc tgtgatgatg gctttagcga tagccagtcc taaaccacta tgggtttcac
      781 tttcctggcg cgaacggtct gcccggtgaa aacgttcaaa aatataaggc aagttagcag
      841 ggtcaatacc ggggccagaa tcgactattt cgagcagcat ataagcagtt tcttcactta
      901 aacgtaaaat aacctgactt ccttccgggc tgtattctat ggcattattt aaaaggttag
      961 atagggcttg aatcattcgg tcttcatcga taagcacttt tttggctgtg ggtaagagtt
     1021 caagttttag ggtgatagat tttcgctcag cctggttttc aaagagtttg ccgcagtgct
     1081 ccaagagttc atttgggtct gtgagttgtt tttgaaggtt taactcgcca gtgtcagcaa
     1141 cagataaaaa ccgtaaatca tcgaccattt ttgaaagacg catggtttca tcggcaataa
     1201 gccccagacg ttcaggtgta acggctaaat cgccatcgcg cattgattca acataaccgt
     1261 tgataaccgt caacggcgta cgcaggtcgt gggcaatatc agcggtcatt tgcttgcgta
     1321 agcggtcgct ttcttcaagg tgatcgctca tttcgttgaa gtttcgcgcg agcgtgccta
     1381 tttcatcttt cccgaggtcg ggtacttttg tgcttagttc gcgctgggca atgcgttgac
     1441 tggctttgct taaggtttca atgggtttgg tgatgcttcg cgcgataaaa aatgcgacta
     1501 tgcccgcaag aactatcgcg ccagccagag cgattatcaa tgcgatgttg cttgagcgca
     1561 caaaacggct ctctacttcg ctataacccg gaaattgatt aggtgaaagt agataaccca
     1621 ccgttttttc atcaatgatt atcggtgtac ttgtagccag ggcttctgca ttcaaaactt
     1681 cgcctggatt tggactgtca aatgatgaaa tgagaactgt tgcatctgac gcagctaagc
     1741 caaaaaggcg gcgagatatt aaagcaccgg tttccgattg actgtccttg ggatgattgc
     1801 tatcgacttg tccttgccgg cgtcccctgc cttgattgct tggccctgca ctttgcccga
     1861 aaccttgtcc ctggctctga ctgcgagata tgcttgaaaa atagatttgc gcgccatccc
     1921 agttttgatg ttcctggtaa tgattagtca ggactgaaat cagttcctca cgttgcttgt
     1981 cgataacaaa ttggttgaac tgtctttgat taaagcgatt aaagacgaac accagaacag
     2041 caccagtgat gagagcgacc aggacaaagg cgagtaataa tttggttttt aggcttgttt
     2101 tcatagcgtt ttatttattc agtcggtaac ctacaccaaa cacggtggtg attaggctgg
     2161 gattagcggg gtctttttct actttggcgc gtaaattacg cacgtgcacg ttgagcgtgc
     2221 tttcaagtcc catgaaaccc gtttcgtaaa gacggtcaac caagtccgcg cgtgtaaaga
     2281 ccttgtttgg ggagcgcata agaatgctta aaagttcaaa ttcaatgggt gttaatagga
     2341 cagcttcctt atttatgctt acggtgtgag tggtaacatt taagaccacg tcaccagctt
     2401 tgagaatttc ttctgcttca acgcttgggt tggcccggcg caaggcaaca cgcacgcgag
     2461 ctgcaagttc gcgcatactg aaaggtttca gtacgtaatc gtccgcgcca agttcgagtc
     2521 cttgaaccgt gtctgtttct tctgttttcg cggtcagaac gataacgggt acctggctct
     2581 cttttcggaa ggcttgcagg aattggtagc catccagtct gggcatcatg atatccagca
     2641 gaattaaatc cgggttttcg tagcgggcta caaagagccc ttcttgtccg tctttagcag
     2701 ttagcacatg gtagccttgc tcgctgaaat aatccttgag catattgcgc aggttttgtt
     2761 ggtcgtcgat gataagaata gtttctgcca tgatgattga gtatagcggt aatttattta
     2821 gatttgatgt agactctcaa attaattttt gatgttcgtc tagtaagcag tccgaaaaca
     2881 gattaaaggt cttcaaccaa actcgccatc acctggctaa tatcgccggc aatgaccagg
     2941 tttgcccagt tgtcataagc ggttgggtcc tggttgatga gcaggagttt gtcaccgcga
     3001 tagtagcgta ccagtgatgc agccggatat actaccagcg atgtgccacc gatgatgaac
     3061 atatctgctc gtgtgatagc tttgatggac gcgtttagca catcagtgtt gagcatctcg
     3121 ccataaagca ccacatcagg gcgcacaatg ccaccacaag tgcagagggg gacttcctta
     3181 gtgcgcatta catatgtcac gtcgtaatct ttgtggcaac ggatgcaata gtagtgttcc
     3241 atggtgccgt ggagttccaa aacattgctt gagccggcgc gttggtggaa attgtcgata
     3301 ttttgggtga tgaccgcttt tagtttgcct agtttctcaa gttttgccag ggcatagtga
     3361 gcggcgttag gctgggcgtc gggataatag agattctcgc gcaggtaggt gtaaaagccc
     3421 ttagggtcag ctttgaagta gtcgtaactc aacaaatatt ccggcgaata gttggcgttg
     3481 tgggcgcggt tataaagtcc ggttgcggag cgaaaatcgg gaataccgga tgcagtgcta
     3541 acgcctgcac ctcccaaaaa gacgatatta tccgaacttt taatgaattc ccgcgcgaga
     3601 ttgatttttt cgttcatgcc ttaatttgct ggtcttgttc tggggcgggg gcgatttcaa
     3661 cctgccccat tttcttgccc agtttattga gtggtgggaa ccagttgacc aaaagtgcca
     3721 cagcgatgcc gatgagcgta tcagcgaccc gttcaagtgt gtaggcgatg ggggggatat
     3781 ccgttgtttt gagcgtaaca atcaggtaaa caatcaccgt gatggctatc gggccaggtg
     3841 tcttaatcca aatcatgatg gtcattaaag gcagggagag caaggcggtg accaggtagt
     3901 aataccattc acgggtatca acggtgacat ttgggaaaat aaagttgagc actaaaaaac
     3961 cgtaaatgcc agcgatgatg gtgccgatga cgcgttcgcg ggcagttttg gcggtagatt
     4021 ttacggtggg ttgcatacag accacggcgg cgatactgct gtggtagact gtgccggttt
     4081 tttggcgaat ggcttctatg ccaaggcaga taaataccgc aatcatggtt ttgataacgc
     4141 gcataccggg cgtattttta atcagaaagg cattcagctg atgagcaaaa tgcttcagct
     4201 tcgcgttttt ttgtgggtct tgggtgacct tttcggtttc tttttcagta ttgttatcca
     4261 tattatttca gaataatcat acccgataat gtgggttttg gggcagttat tctaattata
     4321 gcgggatgtg atgtttgcag ccaggaaggt acaagtatga caaaatgagt gcagttatat
     4381 aaaaacctta aaactttagt gaagaatgat ttgaggcgag tataataact gcggaggatg
     4441 atatgttccc agtagcacac ccaaaaacaa tgcaaaaagc gcgcgaattg ctggcgctga
     4501 accccatctt tcttgatacg gaaacgaccg gaactggcgc gaacgatgtc attattgagg
     4561 tcggcatcgt tgatattaat ggcgatgttt tatatgagag tttggtcaac ccaggtatgc
     4621 cgataccgcc tgaatcaagc gcggtgaatg agataacgga tgaaatggtc gcggataaac
     4681 cgggctgggc gcaagtttgg cgcgaagttg aaccgatttt acgcaaccgc gcgattggaa
     4741 tctacaatgc tgattttgat atgcgtttat taaagcaaag ttgtgcttat gctggggtgc
     4801 catggacttt ggacgacaag caggcttttt gtgtaatgcg gctttacgcg gcgtggtatg
     4861 gcgaatggaa cgcgcgctat aacggctttc gttcgcaaaa actggcgtcc gcgggtggat
     4921 tctgtggcat cgatttgccc aacagtcatc acgcggtgga tgacgcacgt ttgacagccg
     4981 tgcttttgcg ctatctggca gattatgtga aagattaatg gggaattccg acgcaattaa
     5041 ataatgaaaa gctcatagaa ttagttcgct tccatacctt aagtgattaa aaagcttgat
     5101 tttaatggtc gtaacacatc ctagataagc tgaatcatgg tgcgcatcaa tgttatacaa
     5161 aggcttttac acatcaattg ataatcgtat aatcctcaaa aaaaggagtg tctgagtaac
     5221 agacatggaa aagaactctg agaataagat taaaaaccta ccgccaaacc tttgggcggt
     5281 gagtataacc agttttttga tggacatatc cagtgagatg gttattaatg ttctgccttt
     5341 gtttttgttc agcgtattag gcgtgaaaac aaatatcatc ggtattattg agggcattgc
     5401 cgaagctaca gccagtattt taaaagtgtt ttcaggctgg ctttctgaca aaatgcggtc
     5461 acgcaaatgg ttagctgtct taggatatgg actttcggcg attgttaaac ctctgtttta
     5521 ttttgccaac agctggagtg cggtggccgc tatacgttgg ggcgatcgcg ttggcaaagg
     5581 aattcgcact gcgccccgtg atgctttagt ggcagatacg gttaagccag aacaacgcgg
     5641 gttggcgttt ggattgcaca gagcggcaga taccgccggt gcgatgttag gcattctcat
     5701 cgctatcctg gttgtttggc gtgttcaagc gacaggtatt ggcttgcagg aaaatacatt
     5761 tcgcaccatt gtgcttatca gcattattcc tgctgtcctg gcagttctgg cacttatttt
     5821 tggcacccat gaggtcaaag ttgaaggtgt aagacaagcc cctaaaatca gtttcaaggg
     5881 ccttggcaaa aactttcttg tctttatgct cattgtcggt atttttgatt taggcaactc
     5941 gtcggatgct tttcttgttt tgcgggcaca ggaaagaggc atgagtgtgc tcaatattct
     6001 catcatgctg gctgttttca acctggttta tgctcttgtt tcaacgccgg caggcggcct
     6061 ttctgacagg attgggcgta gaaaagtgat tattggcggt tggattattt acgcactcat
     6121 ttatcttggc tttgcccttg ccaaaaatgt gacccatgtt ttcctgcttt atgttgctta
     6181 cggcgtttat tatggcatgg cttatggcac cgcgaaagcg atgattgggg atctggtacc
     6241 tgaagagatg cgtggcacgg cgttcggaac ctataatgct gttttgggta tattggattt
     6301 tcccgcttct atgattgctg gctttttatg gagtggcgtg ggcaagtggg ctgggtttgg
     6361 cgcatccgca ccattctatt tcggtgcgat tatggcgaca ttggctattc tgctctttgt
     6421 cttttggaaa cctgaacctt tgtcagcagc tgaaaacggt taagctgaga cctttgtgac
     6481 acttaaagag agaaatcgga caataggata taatctgctt gaacgatttc tctctgaaag
     6541 gaacatacaa tgcgaatcgg tatattagat taccgcatgg attattacac cataaaacgg
     6601 attatcttga acaaagtacc gggctttgat tatgttcctg ttaaagattt atatagcata
     6661 tccaggagag tggcgcttag attcaaccgt ctttttgaga aaacactctt ttcaactttt
     6721 aatctgaata atcaatttga agatttcgat ttaaacaagg ttgatgtttt gcatttttca
     6781 aatggcatca gctacggtaa aacgccttgg gtatcccagt ttgagactat ccttccccgt
     6841 tttgattatc ttttgcagcg tg
//
LOCUS       DAOSFC010000039         6834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_9687_length_6834_cov_5.146482, whole genome shotgun sequence.
ACCESSION   DAOSFC010000039 DAOSFC010000000
VERSION     DAOSFC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9687_length_6834_cov_5.146482"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK791_01815"
     CDS             <1..407
                     /locus_tag="PK791_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92080.1"
                     /translation="KRIGRFPAAQTDYAKLRRGYVHIPQQASFWRASLWHEVGPLDDS
                     FYFAMDYDLWTRLAAKAPLVYIKEPWAAFRLHGTAKSIAEDDRCWPEMMRVHYRDGGK
                     KLSWINAKYHLRKLLRPILMWKRRRQMTRAKE"
     gene            412..1221
                     /locus_tag="PK791_01820"
     CDS             412..1221
                     /locus_tag="PK791_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOH92081.1"
                     /translation="MLINKSKTAMRVLREKGLASVFNLLKHHFGFAKHVDEGTLILDW
                     FKFISFTGTLVDIGAHRGYDTIPFASEGWKVFAFEPDPVNRAQLEQLTINLSSVHVDA
                     RAVADKSAQQVAFYKSCESDGVSGLQAFLPSHQAEYHVDVTTLTEFVLEKAIDEIDFL
                     KIDTEGYDKMVLDGIPWESLRPRLILCEFEDKKTLPLGYSYHDLANSLLEKAYHLIVS
                     EWKPIKRYGEKHDWRGFYPYPHELQTPKAWGNLIAARDKADYESLSQFLKV"
     gene            complement(1294..2487)
                     /locus_tag="PK791_01825"
     CDS             complement(1294..2487)
                     /locus_tag="PK791_01825"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOH92082.1"
                     /translation="MTDKLSWIDDEIANLKETGFYNTIPTMDSPQGAKVNINGKKVLN
                     FCANNYLGLANHPRLVQAAKDAMDKYGLGPGAVRSIAGTMSLHLELERRMAEFKRVEA
                     AITFQSGFTANGATIPALVGKGDAIFSDELNHASIIDGSRLSGAKIHRFAHADVDDFE
                     RVVKEAQGSYNKGLMITDGVFSMDGDYAPLDKLVEVSEKYGLMTAVDDAHGEGVMGKG
                     GRGIVDHFGLHGKVDVEIGTFSKAFGVVGGVVAGNARIIEWLRQRGRPFLFSSAVTVP
                     DVAACIAAVDLLEESSELVDRLWSNARFFQEEMRKLGFNTGVTQTPITPVMLGDVQLA
                     RQFSRELLEEGIFAGALGFPTVPRGKARIRVMISAAHSQDDLEQALDAFERIGKKLGK
                     GTMEE"
     gene            2701..4170
                     /locus_tag="PK791_01830"
     CDS             2701..4170
                     /locus_tag="PK791_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOH92083.1"
                     /translation="MLKRISSILVFLLFITIVSSCPNSLSPANAQSAYVPGFSCYRDL
                     STIYDRIQQLQYKYPDLLTVTDIGDSWEKQNLGMNHGHDLLILKLEANPSPKPTLILI
                     SGLKANSFGPVEINLRFTEFLLSAYPKQSDVRYLIDHFAVHFLFVANPDGRTKAESQA
                     DGTGDTWTITWAKNTNPSGCASDDGGVALEHNFAYQWNNLPTDPCAADYPGSQAFSEP
                     ETQAIRDYLQGLVQTNPNNHLLINLESYKDFILNPYLYSKTHKPTRYEDYQVLANKLA
                     YGQEALPLEHNNTAIPEKYGTLIDYAEEIQDIKAIQYRLGSQMGGGNVSQCAYFEEVL
                     VEPALQSLLRALKTLPEPLEYGAGPEIHNLSVNLNFNESLIEISGQLDAHNYYHHTNR
                     TTSISALRYSIDLPPWYPDAEENLIDFERDLEYPGLADFFFQLDMNTINPEGATIYFQ
                     GEAEDCTNGYKRKGFVEMVTIKLPNPELNLYLPLISTSR"
     gene            complement(4211..4348)
                     /locus_tag="PK791_01835"
     CDS             complement(4211..4348)
                     /locus_tag="PK791_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92084.1"
                     /translation="MEKPIFFNITLVERFLLLILLIYRENQPLLLNKKGIIHIIPSPY
                     Y"
     gene            complement(4503..5540)
                     /locus_tag="PK791_01840"
     CDS             complement(4503..5540)
                     /locus_tag="PK791_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92085.1"
                     /translation="MMLITQDLLIDITKDYINRLLRQNNDIVACYLTGSVLGDQAFIG
                     GSTDIDLVVVHKEDPPAPREIRRVKSNISLDIVHHHQSLYTFHRRMRQDSWLGYALRN
                     HDGIIYDTDHWLEYIQAGVDAQFDSPENTHARALKFADDARRYWFALDDDDETPFSDW
                     LDLFFKSVDNACNAVAALSGPGLTQRRFLLDFPARAEAVNQMPLVGDLARLIGNDKIS
                     VDFYNQWRPVWETRLVECSQSPHCPPNLHKARKAYFLDCCDALAQSDNLFAVLWPMTV
                     TWRQIAQCSRVGLDEDDTTETEATTDWLSFASQIGFEPAQKNIKIRELGHFIEKIQNI
                     LEDYQREFGLL"
     gene            5738..6616
                     /locus_tag="PK791_01845"
     CDS             5738..6616
                     /locus_tag="PK791_01845"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOH92086.1"
                     /translation="MPNEPFIPKNILVMPNAGVPKAMAIAKEIHKHFIDIKVNSDIFA
                     YDFDGKSEKPKLVDSDLVIVIGGDGTLLRAGHLCAEKEVPLLGIQAGTLGFLVEIKDK
                     EWPKQIDRLMKGDFRIETRMMLNAELVRNDIVNGRWDVINEIVISRGSDVRPIEVRVD
                     LNEGYLASYKADGLIVSTATGSTAYAFSAGGPILPPELRNMLILPISPHLSLDRGVVL
                     AEGATVTLRKKSASDAILSVDGMPPIYLSNTDRVVVSANKNSLKMVRFGEPNYFYRDL
                     ATVLRLNPILKINAND"
     gene            6609..>6834
                     /locus_tag="PK791_01850"
     CDS             6609..>6834
                     /locus_tag="PK791_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92087.1"
                     /translation="MTDNNNPENKTPAIPSDFDELMRPANPETSPEEEPVLRCNRCGT
                     PITPKNSVLTETGYRCKDCIRGHQQVFDISK"
BASE COUNT         1924 a         1576 c         1499 g         1835 t
ORIGIN      
        1 gtaagcgcat aggaaggttc cctgccgcgc aaaccgatta tgccaaactg cgccgtggtt
       61 atgtgcatat cccgcagcaa gcgtctttct ggcgcgcgtc actgtggcat gaagtcggtc
      121 cgctggacga cagcttctat ttcgccatgg actacgacct ttggactcgt ttggccgcca
      181 aagcgccttt ggtttacatc aaagaaccct gggcagcttt tcgtttacat ggcacagcaa
      241 agagcatcgc ggaagatgac cgttgctggc cagaaatgat gcgcgttcat taccgtgatg
      301 gtggtaagaa gctgtcctgg attaacgcta aataccatct gcgcaaacta ctacgcccaa
      361 ttttgatgtg gaaacgccgc cggcaaatga cccgagccaa ggaatgagcc tatgctgatt
      421 aataaatcca aaactgcaat gcgcgtcttg cgcgaaaaag gtttagcgtc agtatttaac
      481 ctgctaaagc accatttcgg ctttgccaaa cacgtagacg aaggaacgtt gatattagac
      541 tggtttaaat tcatatcctt tacaggcacc ttggttgata tcggcgcgca ccgtggctac
      601 gacacaatac cttttgcaag tgaaggatgg aaagtatttg cttttgaacc cgaccctgtt
      661 aatagagctc agttagagca actgacaatt aatttaagtt cagttcatgt agatgcgcgg
      721 gcggtagcag ataaaagtgc ccaacaggtc gctttttata aatcatgtga atcagatggc
      781 gttagcggac tgcaagcctt tttaccttcg catcaggcag aatatcacgt cgatgttaca
      841 acattaaccg agtttgtcct ggagaaagct attgatgaaa tcgatttcct gaaaatcgat
      901 accgaaggct acgacaaaat ggttttggac ggcattccct gggaaagcct gcgtccgcgc
      961 cttattttgt gtgaatttga agacaagaaa accttgcctt tgggctatag ctaccacgat
     1021 ttggcaaatt cattgctgga aaaagcttat catctcatcg tttccgagtg gaaacccatc
     1081 aaacgctacg gcgaaaaaca cgactggcgc ggattctatc cctatccgca cgaactccag
     1141 actccaaaag cctggggaaa tctgatcgca gcgcgcgata aagcggatta tgaatcttta
     1201 agccaattct taaaagttta aatctgtttt attaatacaa aaagtggtct ctgatattat
     1261 gagaccactt tttcatttat tcagaaatat tgtctattct tccattgtgc ctttgccaag
     1321 tttcttgccg attcgttcaa aagcgtccag tgcctgttcg agatcgtcct ggctgtgagc
     1381 tgcagaaatc attacgcgaa tgcgtgcctt gccacggggc acggttggga agcccaaagc
     1441 acctgcaaaa atgccttctt caagcaattc gcggctaaac tggcgcgcca gctgcacgtc
     1501 accgagcatc acgggtgtaa taggtgtttg ggttacgcca gtgttgaaac cgagtttgcg
     1561 catttcttcc tggaagaagc gcgcgtttga ccataatcgg tcaaccaact cgcttgattc
     1621 ttccagcaaa tccacagcgg cgatacaagc agccacatcg ggcacggtca ctgcggatga
     1681 gaataggaaa ggtcgtccgc gttggcggag ccattcaatg atgcgcgcgt tacccgcgac
     1741 aacaccacca acaacaccaa aagctttaga aaaagtacct atttccacgt caacttttcc
     1801 gtggagaccg aaatgatcca cgataccacg accaccttta cccatcacac cttcgccgtg
     1861 agcatcatca acagcggtca tcagaccata tttttctgaa acttcgacca gtttatccaa
     1921 tggggcatag tcgccatcca ttgagaaaac accatcggta atcatgagac ctttgttata
     1981 gcttccttga gcttctttaa caacgcgttc aaaatcatcc acatcagcat gcgcaaagcg
     2041 gtgaattttc gcgccagaaa gtcttgagcc atcaataatt gaggcgtgat tgagttcatc
     2101 tgagaagata gcatcgccct tgccaaccaa tgcggggatt gtggcaccat tggcggtaaa
     2161 accagactgg aaagttatcg ccgcttcaac gcgtttgaat tcagccatgc ggcgttccaa
     2221 ctcaaggtga agagacattg tgccagcgat tgaacgcacc gcgccaggtc caagtccgta
     2281 tttgtccatg gcatctttgg cggcctgtac caaacgcgga tgattagcta aaccgagata
     2341 gttattagca caaaagttga gtactttttt accattgatg ttcactttcg cgccctgggg
     2401 cgagtccatg gtgggaatgg tattataaaa acctgtttct tttagattgg cgatttcatc
     2461 gtcgatccaa cttaatttat cagtcattag cacctcattc ttattgtatt tgttcaatat
     2521 catgattata ccgccttaaa ccttgctatg ggacgattac ttaattgttt ttttgattca
     2581 cctttctaat ctaaccttga aattgaccta aattatgaaa attccaaact ttatttccat
     2641 aacgcacatt aatgtaatgc gctatacgcc agtaatgata gaataaatgg cggtgaagat
     2701 atgttaaaaa ggatttctag cattcttgtt tttcttttat tcatcacaat agtttcttca
     2761 tgccccaatt cattatctcc tgcaaacgca caaagcgcat atgtacccgg cttttcctgc
     2821 tatcgcgacc tgtcaacaat ttacgatcgc attcagcagc ttcaatacaa atatcctgat
     2881 ttgcttactg tgacagatat cggcgactct tgggaaaaac aaaacctggg tatgaaccac
     2941 ggacacgatt tactgatatt aaaactcgaa gccaatccat caccaaaacc aactctcatt
     3001 ttgataagtg gcttaaaagc caattccttt ggacccgtgg aaatcaatct ccgctttact
     3061 gaatttctat tgagcgcata tccaaagcaa agtgatgtcc gctatctgat tgaccatttc
     3121 gcggttcatt ttttgtttgt cgccaatcca gacggacgta ccaaggcgga aagccaggct
     3181 gatggaactg gcgacacatg gacaatcacc tgggctaaaa atactaatcc cagtggctgt
     3241 gcttcagatg atggtggcgt agcgcttgaa cataattttg cttatcaatg gaacaatctg
     3301 ccaaccgacc cttgcgcggc tgattatcct ggttcacagg ctttttcaga accagaaaca
     3361 caagccatcc gcgactatct ccagggactc gttcaaacaa atccaaataa tcacttactc
     3421 atcaatctgg aaagttacaa agattttatt ctcaatcctt atctgtattc aaagacacat
     3481 aaaccaacgc gatacgaaga ttatcaagtc ttagccaaca agctggctta cgggcaggaa
     3541 gctttgccct tggaacacaa taacaccgcc attcctgaaa aatacggcac gctaatcgac
     3601 tatgccgaag aaatacagga tataaaggcc attcaatatc gtttaggaag ccaaatgggt
     3661 ggtggcaatg tatcacaatg tgcttatttt gaagaagttt tggtagaacc cgccttgcaa
     3721 agtcttctgc gcgccttaaa aactttgccc gaaccactgg aatacggtgc tggtccggaa
     3781 attcataatc taagtgtgaa cttgaatttt aatgagtcgc tgattgaaat aagcggacag
     3841 ctagacgcgc ataattatta tcaccatacc aaccggacga caagcatttc cgctttacgt
     3901 tacagcattg atttgccacc ctggtatcct gacgcagaag agaacttaat agactttgag
     3961 agagatttag aatatccagg attagctgac tttttctttc agctggatat gaacaccatt
     4021 aatcccgaag gtgctaccat ttattttcaa ggtgaagctg aagattgtac aaatggttat
     4081 aaacgtaaag gttttgttga aatggtgaca atcaaattgc ctaatccaga gctcaatctt
     4141 tatttgccac ttatcagcac gagccgttaa aagattattc tttaatctct tttaagtaag
     4201 ttaagtagtt ttaatagtag gggctgggga tgatgtggat aattcccttt ttattcaaaa
     4261 gcaaaggctg attctctcga taaattaaca aaatcagaag caaaaatctt tcaacaagcg
     4321 tgatgttgaa aaagatcggc ttttccacaa ttccacagac aagtgcttgt caaaaattaa
     4381 aaggacaaac aatacacatt ttatgcagaa cttatccaca gctaaacagg cgactattgt
     4441 ggattaaaaa caaaaaactg cggttttatc cacagttttc caccacttat ccacggtttt
     4501 gtttagagaa gaccaaactc acgttgataa tcttccaaaa tattttgaat tttttcgatg
     4561 aaatgaccca gttcgcgaat ttttatattc ttttgtgcgg gttcaaagcc aatttgactg
     4621 gcaaaactca gccagtctgt tgtcgcttca gtttctgttg tgtcgtcttc atcaagcccc
     4681 acgcgtgaac attgcgcaat ctgccgccag gtaaccgtca tgggccaaag gacagcaaat
     4741 aaattgtctg actgtgccag ggcatcgcaa cagtccaaaa aataagcctt acgcgcttta
     4801 tgcaggttgg gcggacaatg cggactttga ctgcactcca ctaaccgtgt ttcccaaaca
     4861 ggacgccatt gattataaaa gtccactgaa atcttatcat taccgattaa acgggccaaa
     4921 tctcccacca agggcatttg attcactgct tcagcgcgtg ctggaaagtc gagtaaaaag
     4981 cgtctttggg tgagtcctgg tccgctgagt gcggccacgg cgttgcaggc attgtcaaca
     5041 gacttgaaaa acaaatccag ccaatcgctg aaaggcgttt catcgtcatc gtccagcgcg
     5101 aaccagtacc ggcgggcatc atcggcaaat ttcagcgccc gcgcgtgtgt gttttcggga
     5161 gagtcaaact gcgcatccac gccagcctga atatattcaa gccaatggtc tgtgtcgtaa
     5221 atgatgccat catggtttcg cagggcatag cccagccagc tgtcctgtcg cattcggcga
     5281 tgaaaggtgt aaagcgattg atggtgatga acaatatcca gcgaaatatt gcttttaacg
     5341 cgtcgaattt cgcggggagc gggtgggtct tccttatgaa cgacaaccaa atcgatatcg
     5401 gtgctgccac caataaaagc ctggtcgcca agcaccgaac ctgttaaata acaagcgacg
     5461 atgtcattgt tttgacgcaa caggcggttg atatagtcct tggtgatgtc aatcagtaaa
     5521 tcctgggtaa tcagcatcat ttttctgtat ttcctaaacg tttaaaatat accataaagc
     5581 catcgggtaa ctgacttatt tcgcaaaatg gctgacgcat aggcggattt gtttaaatca
     5641 ttgtctttga cggatttaat aatcaggtac tgtaataata gagagaatga tgattctttt
     5701 cttgttcaag ctgaatgttt agggctataa tacagccatg cccaatgagc cctttattcc
     5761 aaagaacatc ttagtcatgc caaacgctgg agtgcccaag gcgatggcta ttgccaaaga
     5821 aatacacaag cattttattg atatcaaagt gaacagtgat atttttgcct acgactttga
     5881 tggcaaaagt gaaaaaccaa agctggttga cagcgatttg gtcattgtta tcggtggcga
     5941 tggcacgttg ttgcgggcag gacatttatg cgctgagaaa gaagtgcccc ttttgggcat
     6001 tcaagcgggc acactcggtt ttttggtgga aattaaggat aaagaatggc caaaacaaat
     6061 tgaccgcttg atgaaaggcg actttcgcat cgagacgcgc atgatgctga atgcggaact
     6121 ggtgcgtaac gacatcgtga acggccgctg ggatgttatc aacgaaatcg tcatcagccg
     6181 tgggtccgat gtgcgcccga ttgaagtgcg cgtagacttg aacgaaggct atttggcgtc
     6241 gtataaggcg gatggcttga ttgtcagcac ggctaccggt tctaccgcgt atgccttttc
     6301 tgccggtggg cctatcttgc cgccagagtt gcgcaatatg cttattttgc caatctcgcc
     6361 ccacctttcg cttgaccgtg gtgtcgttct ggcggaagga gccacggtga cgcttcggaa
     6421 aaagagtgcg agtgacgcta tcttgagcgt ggacggtatg ccgccaatct atttaagcaa
     6481 cactgaccgt gttgtggtca gtgccaacaa aaactctctt aaaatggtgc gttttggtga
     6541 gccaaactac ttctatcgtg atttggcaac tgtattgcgc ctaaatccca tcttgaagat
     6601 aaatgccaat gactgacaat aataatccag aaaataaaac ccctgccatc cccagtgact
     6661 ttgacgaatt aatgcgtcca gctaatcctg aaacaagtcc tgaagaagag cctgttctgc
     6721 gatgtaatcg ctgtggcacg cccataacgc caaaaaactc ggtgctgacc gaaacaggct
     6781 atcgttgcaa ggactgcatt cgcggacatc agcaagtttt tgacatctcc aagc
//
LOCUS       DAOSFC010000040         6681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_9978_length_6681_cov_4.661334, whole genome shotgun sequence.
ACCESSION   DAOSFC010000040 DAOSFC010000000
VERSION     DAOSFC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9978_length_6681_cov_4.661334"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PK791_01855"
     CDS             complement(<1..421)
                     /locus_tag="PK791_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOH92088.1"
                     /translation="MSSFANLPKYLFTSESVTEGHPDKLCDQVSDAVLDELLRQDPFS
                     RVACETATKTGYVMVLGEVTTNGFVDIDSLARSVIKDIGYDRGKKGFDAEVCGVLTAL
                     AGQSPDISQGVDRKGRTQDVEYTEEYINAGGAGDQGMM"
     regulatory      complement(491..589)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            742..1002
                     /locus_tag="PK791_01860"
     CDS             742..1002
                     /locus_tag="PK791_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92089.1"
                     /translation="MARALTPETRIKKARALIEDARKIEKPQSVGWAFFSYTANVKDN
                     LRKAFELVKLINHSPATPEDVKADARAVIEEISQAEKEILGK"
     gene            complement(1015..1767)
                     /locus_tag="PK791_01865"
     CDS             complement(1015..1767)
                     /locus_tag="PK791_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92090.1"
                     /translation="MENTQKFSGKSEHYEQSRPAYATEFITWLADTLELDANTPVADF
                     GAGTGKLTRQLLELGLPVYAVEPNPDMREKMIALLNGYANFHPIAAPAENSGLAEKSV
                     KLITAASAFHWFDTHAFRAECQRILWQDGWVCLVWNRRQLDNQINQGFRDIFGRYCPN
                     FSGFSFEQQKTTQAINNFFKDAPLVKHFDNPVRYDLSGFVGRALSASYALVASDVGYA
                     EFVGDLETLFHQHAIDGFILMPHQSVAYLGKV"
     gene            1845..2726
                     /locus_tag="PK791_01870"
     CDS             1845..2726
                     /locus_tag="PK791_01870"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOH92091.1"
                     /translation="MEQSYIPPRQKQLAWNVVIMVGLVLLGVGLIVLANLSTLIRYAS
                     VFVAVAFLLVVPFFHIFNQYNQAKQIRYLIDDESLRIHWGVNKLSLPLKQIDWAHHLS
                     EFEQQMPLPRWHLPGVYFNLFEVHGMGKTQFVATDPQRMILIKADGRYVVVSPEDPAA
                     FLARLQERRNLASPSAATIEAENLKSFLLRSWRDKGIKRWLIVSLVMLIVLWIVAGLM
                     AALRQTVTWVTLEEVRAIQLLLLPIFGTFMWIVSLVLSFFILTDELAEKAFVYILLIS
                     TSIVCLILTVAGLVMAL"
     gene            2787..2860
                     /locus_tag="PK791_01875"
     tRNA            2787..2860
                     /locus_tag="PK791_01875"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2821..2823,aa:Val,seq:cac)
     gene            3149..4867
                     /locus_tag="PK791_01880"
     CDS             3149..4867
                     /locus_tag="PK791_01880"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92092.1"
                     /translation="MKKKFLSIVIIALMWIGILYFAFGSELGTNAMADIHEKYPDNII
                     DFDDYSQSDDPIESSRGYVKPESRIFTPPSEESLKLTREKVQAFDCGTIEGATIRECQ
                     ALVALYASTNGAAWTNNTNWLESKAVGEWYGIRTNYENIWGLVLNENNLSGTLPPEIG
                     NLSELDYLALSKNFISGNLPSELSQLEKLEYLHLDDNLLNGPILSDIGMMSNLKIIRL
                     NNNLFSGQIPASLGHLNHAWELVLCNNKLTGRIPAELGQVGYLWHLDLSGNQLSGPVP
                     SELGDIRGLGSLLLSSNQLSGNIPPELGNLTNLYYLAIADNMLSGSIPAEFVNLSKLE
                     YLFLQNNRLSGTIPAEIGALTNLITLNLSHNKFEGAIPESFTNLVNLGKYTDLGYNRL
                     NVPQAEPVQSFLNEKDPDWYLTQAIQTTIPCDLGGEIFLHNNRVEITIPAGACDGELD
                     VLLTPLVGPSHDYKPMYWGKNSFELSAWNAHGSVTQFLKPLSFTIYYFEDDFNVMPEE
                     ELVVKLFDKDYAVWRDAISTCPDGEYTRNFDENWLRLPVCHLTEFGLFNLLELPFRLY
                     LPIGLK"
     gene            5044..6462
                     /locus_tag="PK791_01885"
     CDS             5044..6462
                     /locus_tag="PK791_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92093.1"
                     /translation="MKKKIIGISIIALLLIAILFATFNSDLGVNAMEKINANYPDNII
                     IFDDIAEQSEGDDLPEPRVFIPPSEESIKLTLEQVQAFDCETVEDVPVSECEALVALY
                     ASTNGSAWTNNTNWLQSTTVGDWYGVSVTASYNGPTILSLSGNNLVGTIPPEIENLSD
                     LKSLILSKNFLTGYIPSGIFNLVNLLRINLSDNLLTGTIPPEFGNGDYVAINLGSNLF
                     TGQIPAELGEAQSLQYLNLSDNQLTGTIPAEIGQLWYLYTLNIANNKIEGVVPQSLTN
                     LSRLRQGTDFGYNRLNVPQEEPVHSFLNTKDPDWYLTQAIQKPIPCVSGGEIISRNGR
                     VKIAVPAGACNGVLELMLAPLMKPSQAYEPMFWARNSFELSAWDERGPVTQFPKPLNF
                     TLYYSDGGIGTAPENFLTIEMVDEEDIVWRDAVGTCPDGEYTRNFDDNWVRLPVCHLT
                     EFGLFNHRPETPLRLYIPMGFN"
BASE COUNT         1834 a         1447 c         1543 g         1857 t
ORIGIN      
        1 acatcatgcc ctggtcgccg gcaccacccg cgttgatgta ttcttcggta tattcaacat
       61 cctgggtgcg tcccttgcgg tcaacacctt gagaaatatc aggcgactgt cccgccaaag
      121 cggtcagcac accacagact tcggcatcga aacctttttt gccacgatcg taaccgatgt
      181 ctttgatgac gctgcgcgcc agactgtcga tatccacaaa gccatttgtt gttacctcgc
      241 cgagaaccat cacataaccg gttttggtcg cggtttcgca agccacacgg ctaaaggggt
      301 cctggcgcag gagttcgtct aaaacagcat cgctaacttg gtcacagagt ttatcagggt
      361 gtccttctgt aaccgattcc gaagtaaaca agtacttggg taaatttgca aaacttgaca
      421 tataaatctc ctatgtttat tcagtaaaaa gccccgcgat tgaggggcaa gttgttttgg
      481 gatttctcac ctctcatctt ccagttttcc tgtcggattt ggcaccatgc tgtttgtgct
      541 ggttgccgaa gtttcaccgg gccgatccct ccacttctct ggataagagc gctaaaggct
      601 atttagtttt cttaattata cgcgatggcg cctatgcgtc aagcgaaatt gaagccctgt
      661 agcgacatta ggcaccggga attccgttgt ttggtggcgt tggcgtgcta caattagcta
      721 agcagaagca aggagataac tatggcaaga gcattgaccc ccgaaacccg cattaagaaa
      781 gcccgtgcat taattgaaga cgcacgcaaa attgagaaac ctcaatcagt tggatgggcg
      841 ttttttagct ataccgcgaa tgtaaaagac aacttgcgca aggcctttga gttggtcaag
      901 ttgattaatc actcgccagc aacaccggaa gacgtcaaag ccgacgcgcg ggcggttatc
      961 gaagaaatat cccaggcaga gaaagaaatt ttaggtaaat aatttaggct tgtgttagac
     1021 cttgccgaga taagcaacgc tttggtgggg cataaggata aaaccgtcga tggcgtgttg
     1081 gtggaataag gtttccaagt cgccgacgaa ctccgcgtaa cccacatcac tggctacaag
     1141 cgcgtaggat gccgacaatg cccgcccaac aaagcctgat aagtcataac gaacggggtt
     1201 atcaaagtgt tttaccaggg gcgcgtcttt aaaaaagtta ttgattgcct gtgtcgtctt
     1261 ttgttgttca aaactaaaac cagaaaagtt ggggcaatag cggccgaaga tatcccgaaa
     1321 gccctggttt atttgattgt caagctgacg tcggttccaa accaggcaaa cccagccatc
     1381 ctgccataaa attcgctggc attccgcgcg gaaggcatgg gtatcaaacc agtggaaagc
     1441 gcttgcggca gtaatgagtt tgacgctttt ctccgccaga ccggaatttt cggccggcgc
     1501 ggcgatggga tgaaaattag catagccatt caacaaggca atcatctttt cgcgcatatc
     1561 ggggttaggt tcaacggcat agaccggcag acccagttcc aacaactggc gggtgagttt
     1621 gcccgtgcct gcgccaaaat ccgcaaccgg cgtgttcgcg tctagttcca gcgtatctgc
     1681 cagccaggtg atgaattccg tggcataggc agggcgggac tgttcgtaat gttcactttt
     1741 gcccgaaaat ttctgtgtgt tttccatgct tgctccttta atctatttta acttaagtac
     1801 caggatgtgg agacatttct tttaagcttg cgtataattg gagcatggaa caaagctata
     1861 tccctccgcg acaaaaacaa ctggcctgga acgtggtaat tatggttggt ttggttctgc
     1921 ttggcgtggg tttgattgtg cttgccaatt tgtcaacgct aatccgctat gccagtgtct
     1981 ttgtggcggt ggcatttttg ctggttgtgc ctttttttca tatcttcaat caatataatc
     2041 aagcgaagca aatccgctat cttattgatg atgaaagttt gcgtattcat tggggcgtga
     2101 acaagcttag ccttccgctg aaacaaatcg actgggcgca ccatctgagc gaattcgagc
     2161 agcaaatgcc cttgccacgc tggcacttgc ccggggtgta tttcaatctg ttcgaagtac
     2221 acggcatggg aaaaacgcag tttgttgcca ccgacccgca aagaatgata ctgattaaag
     2281 cggatgggcg ctacgtggtg gtgtcgcctg aagacccagc agcatttttg gcgcgattac
     2341 aggagcgcag gaacctggct tcacccagcg cggcgactat tgaagcagaa aacctgaaaa
     2401 gttttctgct tcgttcatgg cgtgataagg gtataaagcg ctggctaatc gtcagtcttg
     2461 ttatgctgat tgtgttgtgg attgtggctg ggctgatggc agctttgcgc cagacggtta
     2521 catgggtcac cctggaagaa gtgcgggcaa tccaactctt attactgccg attttcggca
     2581 cgtttatgtg gatcgttagt ctggttttga gtttttttat cttgacggat gaacttgctg
     2641 aaaaagcatt tgtgtatatc ttattgataa gtaccagcat agtttgcctg attttaacgg
     2701 tggctggctt ggtgatggca ctataaaaat attggcgttg aatcattgga caaatgcctt
     2761 gtgttttgct ataatcgcta cactttgggc gcttagctca gttggttaga gcgcacggtt
     2821 cacatccgtg aggtcactgg ttcgagtcca gtagtgccca tttttaccac tctcatcgag
     2881 agaaaaccta ctaaagacct cacggttttt ctgctaacga catcacggat aaaagcgaaa
     2941 ggagaccagt ttttataaaa aaagctggtt ttttgtgttt tataccggct tgtttttaat
     3001 gagaaaaagc acttaggttc acaatgccag ttaacaaacc aagcaatcac cagctattac
     3061 tcatatttat taagataaca aaaaagccgc catggtatac tagctcttga ttgttaccac
     3121 tttgaacaat cgaaaggacc tgagctctat gaaaaagaaa tttcttagta ttgtcatcat
     3181 cgcgcttatg tggattggca ttttgtattt cgcctttggt tcagagcttg gcacaaacgc
     3241 catggctgat atccatgaga aatatcctga taacatcatt gattttgatg actactcaca
     3301 aagtgacgac cctatagaaa gtagccgggg ttatgtcaag cctgagtcac gtatttttac
     3361 cccacccagc gaagagagcc taaagctgac ccgggaaaag gtccaggctt ttgattgtgg
     3421 aacaattgaa ggtgcaacta tacgtgaatg tcaggctctt gtggcacttt atgccagtac
     3481 caatggcgca gcatggacga ataataccaa ttggctcgaa tcaaaggccg ttggggaatg
     3541 gtatgggatt cgaacaaatt acgaaaatat ttggggcttg gtgctgaatg agaacaatct
     3601 ttctggaact cttccaccag agattggaaa cctctctgaa cttgattatt tagctctgtc
     3661 aaagaacttt attagtggca atctaccaag tgaattaagt caattggaaa aattggaata
     3721 cctccactta gacgataatc tactgaatgg tcccatcctt tcagatattg gcatgatgtc
     3781 taatttaaaa atcattagat tgaataacaa ccttttttcg ggacaaatac cagcttcatt
     3841 gggtcacttg aatcatgcgt gggaattggt tttatgcaat aataaactta ccggacgaat
     3901 accagccgaa ttgggacaag ttggatacct ttggcattta gatttatccg gaaaccaact
     3961 atcaggtccg gtaccgagtg aacttggtga tattcgtgga ttaggcagtt tgcttctttc
     4021 atcaaatcaa ctaagcggga acattccacc ggaattaggc aatttaacta atctctatta
     4081 tctagctatt gcggataata tgctttcggg gtctatccct gccgagtttg tcaacttgag
     4141 taaactcgaa tatctttttc tgcaaaacaa ccgactttca ggaactatcc cagctgaaat
     4201 aggcgcgtta acgaatttaa tcaccctgaa cctttcccat aataaatttg aaggtgccat
     4261 tccagagagt tttacaaacc tcgtcaatct cggtaaatac acagaccttg ggtataaccg
     4321 cctgaacgtg ccccaagctg agccggtgca gagtttcctg aatgaaaaag accctgattg
     4381 gtacctgacc caagccattc aaacgactat tccttgcgat ttgggtggag aaattttctt
     4441 gcataacaac agagtggaaa ttacgatacc agcgggagca tgcgatggcg aactagacgt
     4501 gttactcacg ccactagtgg gtccaagcca tgactacaaa cctatgtact ggggaaaaaa
     4561 cagctttgaa ctaagcgcgt ggaacgcaca cggctcggta acacaattcc taaagccctt
     4621 gagttttaca atttactatt tcgaggatga tttcaatgtt atgcccgaag aagagctcgt
     4681 tgttaaattg tttgacaagg actatgcggt ctggcgcgat gctattagca cttgtccaga
     4741 tggcgaatac acgcgtaact tcgatgaaaa ctggctgcgt cttccagttt gccacttaac
     4801 ggaatttggt ttattcaatt tactggaatt acccttccgt ttgtaccttc caataggttt
     4861 aaaatagtct aaactgaact agatacagag actggctgag aggtcagtct cttattattt
     4921 aacagacacg gccaaaaaac tgcattgaga gaagggctga atgtcgcgcg cgtaaaatag
     4981 tttgactttc aaatgccaga aaaaatgcca tgatatacta gcggttgaaa ggaattgcac
     5041 tgtatgaaaa agaaaattat tggtattagc atcattgcgc ttctattgat tgctattttg
     5101 tttgccacat tcaactcaga ccttggtgtg aacgccatgg agaaaatcaa cgcgaactat
     5161 cctgacaata tcattatttt tgatgatatc gcggagcaaa gcgaaggcga tgacttgcca
     5221 gagccgcgtg tatttatccc gcccagtgaa gagagtataa aactgaccct ggaacaggta
     5281 caggcatttg attgcgaaac agttgaagat gttcccgtgt ctgaatgcga ggctcttgta
     5341 gcactttatg ccagtacaaa tggctcggca tggacaaata ataccaattg gcttcagagt
     5401 acaactgttg gagattggta tggagttagt gttacggcat cttataacgg accgaccata
     5461 ttatcgttat cggggaataa tcttgtcggt acaattccgc ctgaaatcga aaacctttct
     5521 gatttaaaaa gcttaatatt gtctaaaaat tttctaacgg gctatatccc atctggaatt
     5581 ttcaacttag tcaatcttct tagaattaat ctaagcgata atttgctaac tggcactatt
     5641 cccccagaat ttggaaatgg agattatgtt gctataaatt tgggtagtaa tcttttcact
     5701 ggtcagatac cagctgaatt gggtgaagct cagagcctgc aatacttaaa cttatccgac
     5761 aatcagctca ctggcactat tcccgctgaa ataggacaat tatggtacct gtatacctta
     5821 aatattgcca acaataaaat tgaaggcgtt gtacctcaaa gtcttacaaa tttaagccgt
     5881 ctgaggcaag gaacagactt cggatacaac cgtctgaacg ttccccagga ggaaccggta
     5941 catagttttc tgaacacaaa agaccctgac tggtacctga cccaagccat tcaaaaacct
     6001 attccctgcg tctcgggcgg cgagatcatt tcgcgcaatg gtcgggtaaa gattgctgtt
     6061 cccgctggtg catgcaatgg agtgctggaa ctgatgctgg cacctcttat gaaaccaagc
     6121 caggcctacg aaccgatgtt ctgggcgaga aacagttttg aacttagcgc gtgggatgaa
     6181 cggggcccgg tgacacagtt cccaaagccc ttgaacttta cgctctacta ctctgatggt
     6241 ggcatcggaa ccgcgcccga gaactttctt actattgaga tggttgacga agaggatata
     6301 gtctggcgcg atgctgttgg cacttgcccg gatggcgaat atacgcgcaa ttttgatgat
     6361 aactgggtgc gactaccggt ttgtcattta acagaatttg gcctatttaa ccacaggccc
     6421 gagacaccgt tacgtttgta tattccgatg ggttttaatt agcctgaact tgactaaata
     6481 tagggactgg cttttcaacc agtctctttt ttgttaagcc aggtaatata atggtgcgtt
     6541 acatcaaagc tgagttttca caattgacaa attctttgat atgttagcca tattgtgctc
     6601 tccaaaaatc agttcagcca ggcttataaa gttttagaag tttgtcgcaa aataaagtgt
     6661 tataagtccc aaaatacaaa t
//
LOCUS       DAOSFC010000041         6652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10040_length_6652_cov_4.745945, whole genome shotgun sequence.
ACCESSION   DAOSFC010000041 DAOSFC010000000
VERSION     DAOSFC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6652
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10040_length_6652_cov_4.745945"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PK791_01890"
     CDS             <1..562
                     /locus_tag="PK791_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016429 - tRNA
                     (adenine-N1-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HOH92094.1"
                     /translation="ETEHLVDEALPWLRASPSASHALDVGTGSACIAISLAKNHPALT
                     ITALDISEDALNLAKENAQLHGLQDRIAFQQSDLLANFTGQADLICANLPYIPSATLA
                     ELSVSAWEPVNALDGGQDGLAFIRRLLEQSHAVLKTPGLILLEIEASTGEPALDLAKT
                     SYPKANVDLLKDLAEYNRLIRIEVPK"
     gene            559..1191
                     /locus_tag="PK791_01895"
     CDS             559..1191
                     /locus_tag="PK791_01895"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOH92095.1"
                     /translation="MNQPERLSSHDPKAIQRALEVLNAEGLIVFPTDTLYGLACLPNS
                     KRALDKIYATKHRSRAKALPVLIADTTQLTEITHEIPPNAQKLIKHFWPGKLTLILPK
                     KEGKFQHLSTLDGIALRMPNHPFALELLGKSGPLAVTSANLSEHANHADPAEILAELA
                     GHLDLFIDDDILTSTTASTIVDCTQTPPLILRDGAISKAELNTVLGDEIA"
     gene            complement(1554..2516)
                     /locus_tag="PK791_01900"
     CDS             complement(1554..2516)
                     /locus_tag="PK791_01900"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOH92096.1"
                     /translation="MKKKILIFSLIALLLLGACAKAPAQKYEYITEDSTGYAGVPELP
                     RESLLMDEALEGYELGSSTQERLVIKTANMRVSVVDPVEAMWSVSQMATQLGGYVISS
                     ESSSSVNSSGEYKTASISIRIPTQKLDVAMEKIRQLSADGKDGVLSESTSGEDVTSDY
                     VDTESRVRNLEAAEEQLMVLMENTTDLDKTMKVFRELTRTREEIEVLKGHMKYLRESA
                     SMSMISVNFVAEASIKPIEIGGWKPGVTARESLQKLIDIAQGIADVLIRFSIVCLPFL
                     IPLGLGAFFVTKAVKKRRAKKQVQRANYYAKPPVIDPVEPEKKD"
     gene            complement(2585..3310)
                     /locus_tag="PK791_01905"
     CDS             complement(2585..3310)
                     /locus_tag="PK791_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOH92097.1"
                     /translation="MRALVISDIHSNLGAFEAVLADAGEFDRVICLGDVVGYGPDPEE
                     CVSLLRELPNLTCIMGNHDAAVLDIISTTHFNHEARKALNLQKSLMTKSSLDFLSELP
                     EKLLLDGITLAHGSPRDPIWEYVDQGGIAWAAFEYFESQGSLVGHTHTPAIFIESADF
                     KVRLLKPESGDRWIPQERFILNPGAVGQPRDRDPRAAYVIWDLEENSFMFKRVPYDVT
                     AVAKRIEERGIPSIQARRLNLGR"
     gene            complement(3316..4563)
                     /locus_tag="PK791_01910"
     CDS             complement(3316..4563)
                     /locus_tag="PK791_01910"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HOH92098.1"
                     /translation="MKMRRNFLLILLSCALLLSACGIQSGLSAEEAQQTEVANLVAEM
                     MTQQSLDKQKGTEEPEKTEEPTPEATETPVPETPTQEVLPTEPAVYSVGPTNFPEDVN
                     PLTGLKVADPSILKRLPIMVKVPNFPAYGRPHGGLSSADIVFETWIGGGSNRFAPLYY
                     GENSDYVWPIRSIRIVDPEIASLYNAVIAFSGGDPVKVMPKIYRIMGDRLFPEGTCPG
                     ICDRGSGDVTRLYANTAAITEYFQNAGIDPATRPNLDGMKFSEAIPAGGKPASKVEFH
                     FNYYNKGDWEYDEASGNYLRWIEDAYTYEMKPLVDRNTEEQLAFSNVIMLFVEHVEIE
                     TQLIDIKVWGNTAGRRAVIFRNGQAYEAIWKTNDNESPISFYDLEGNPFPLKPGKSWI
                     ALTGLNTVENITGDLWHYDFKMP"
     gene            complement(4614..>4862)
                     /locus_tag="PK791_01915"
     CDS             complement(4614..>4862)
                     /locus_tag="PK791_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOH92099.1"
                     /translation="PEQMRAPIAYLSEHSTKPIAAIPNAGIPQKVDGALIYPVGPEAF
                     AETLLGYVEAFDIRAIGGCCGTRAEHIRALVDGLQRLS"
     assembly_gap    4863..4962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4963..5440)
                     /locus_tag="PK791_01920"
     CDS             complement(<4963..5440)
                     /locus_tag="PK791_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOH92100.1"
                     /translation="MTPEVPKTKTAYLDALQERVLVFDGAMATNLQALLLTTSDCDMV
                     EMVSPYESLVLSAPNLVAEVHRQFLAAGVDVLQTNTFQANRIALAKYGLAHKTDEINT
                     IAALIARDEIDKFSHDGKQRFVAGSMGPSGVFLAGVDAVRNEQTYEDIADVYCQQAR"
     gene            5719..6057
                     /locus_tag="PK791_01925"
     CDS             5719..6057
                     /locus_tag="PK791_01925"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH92101.1"
                     /translation="MKTEIPNKPINLLPPDDENIKRMAETFKGLADPTRLKLIDLLLK
                     GERCVNDLAEATQTSQSAVSHQLRLLRNLRFVSSRRDGQQVFYRINDEHIGELFKLAL
                     DHALHMHERI"
     gene            6057..>6652
                     /locus_tag="PK791_01930"
     CDS             6057..>6652
                     /locus_tag="PK791_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92102.1"
                     /translation="MSEHHHHQSSSCGCSGDTCGLMVTPDPEGVKHSKILDFKGFWQH
                     FWQMPELKLVIPGILLLAISLFLPRFGVSHRWTIIIQLLILPVAGWFVLSHGFKSLFI
                     RRKLNMDVLMSLALIGAVLIGEGTEALILLILFTLSEAIEGYTSASARKVLTEFADLA
                     PKEALRLTNGEESLVPVEELKIGDRIIVRAGDRLPMDG"
BASE COUNT         1717 a         1742 c         1376 g         1717 t
ORIGIN      
        1 tgaaacagaa cacctggttg atgaagctct accatggcta cgcgcaagcc cttcggcaag
       61 tcacgcgctc gatgtcggca ccggttccgc ttgtatcgcg ataagcctgg ccaaaaatca
      121 ccctgcccta accatcaccg ccctggacat ctccgaagat gccctaaatc ttgccaaaga
      181 aaacgcgcaa ctacatggtt tgcaagaccg catcgcgttt cagcaatctg acctgctggc
      241 aaactttaca gggcaagcag atttaatttg tgctaacctg ccatacatcc ccagcgcgac
      301 actcgccgaa cttagtgtaa gtgcctggga gccagttaac gcgctcgatg gcgggcaaga
      361 tggtctcgcg ttcattcggc gcttgcttga acaatcacac gctgtgctca aaacgcctgg
      421 ccttattctg cttgaaattg aagccagcac cggtgagcca gccctggacc tggcaaaaac
      481 aagctaccca aaggcaaatg tggatttgct taaggattta gctgaatata atcgcctgat
      541 tcgcattgag gtacccaagt gaaccagcct gagcgattgt ccagccatga ccctaaagcc
      601 attcagcgtg cccttgaagt cctgaatgct gaagggctaa ttgttttccc taccgataca
      661 ctttacggtt tggcttgtct gcctaattca aaacgcgcat tagacaagat ttacgccacc
      721 aaacaccgca gtcgcgcgaa agccttgccc gttttgatag ctgacaccac ccagctgact
      781 gaaatcacac acgagatacc cccaaatgcg caaaaactaa tcaagcactt ttggccaggc
      841 aaattaacgc ttatcttgcc caaaaaagaa ggtaaatttc agcatttaag caccctggat
      901 ggcatcgccc tgcgtatgcc caaccatccc ttcgcgctgg aattgcttgg caagtcaggc
      961 ccgctcgcgg tgaccagtgc gaatctttcc gaacatgcta atcatgccga cccggctgaa
     1021 atacttgccg aacttgccgg ccacctggac ctttttatag atgatgacat cctcacttcc
     1081 accacagcca gtacgattgt ggattgcacc caaacgccac cattaatcct gcgcgacggc
     1141 gcgataagca aagctgaact caacaccgtt cttggtgatg aaatagccta attcgattcg
     1201 tagttatacg cgttgtatat ttattagcat attttcaatc aagaatatta cgttcacgga
     1261 ggcgtcttcg ccgacgcaaa aaacatctta taagtggatt ttttaatgct gttgctaagc
     1321 aattgcatat caaaatttta gattcaccag cctatattga ccacggcgag acgcctcggt
     1381 gaacagggat ggtaattatc tgcttatttt cacaaacttt ctgccattat taattactgc
     1441 acaatgctaa gtaataagct tgctaactac aatcccattc gcccccaaac actaggcctt
     1501 aaaacagaat cgcgccgtga atacagcgcg attctgtcta tatatcctaa agtttaatct
     1561 ttcttctctg gctcaacagg gtctatcaca ggcggcttgg catagtaatt cgctcgttgt
     1621 acttgctttt tcgcgcggcg tttcttaacc gcttttgtca caaagaaggc gcccaaaccc
     1681 aacgggatta ggaaaggcaa gcaaacaata ctaaagcgaa tgagaacatc cgcaatgcct
     1741 tgcgcgatat caatcaactt ttgcagcgac tcacgcgcag taaccccggg cttccatcca
     1801 ccgatttcaa ttggtttaat tgaagcttct gccacaaagt ttactgaaat catagacatc
     1861 gatgctgatt ctctcaaata cttcatatgc cctttcaaca cctcgatttc ctcacgtgtg
     1921 cgggtcagtt ccctgaaaac cttcatggtc ttatcaaggt cagtcgtatt ttccatcaac
     1981 accattaact gctcttcagc agcttcaaga ttgcgcacgc gagactcggt atctacataa
     2041 tccgatgtaa catcttcacc agacgtcgat tctgataaaa caccatcttt gccatcagct
     2101 gacaattgac gaattttttc catcgctaca tctaattttt gagtaggaat ccgaatggat
     2161 atgcttgcag ttttgtattc gccactcgaa tttactgaac tagatgactc cgaactaatc
     2221 acatatccac ccaactgagt tgccatttga ctgacagacc acatcgcctc taccgggtca
     2281 accactgaaa cacgcatatt tgcagtttta ataaccagtc gttcttgagt tgatgaccct
     2341 aactcgtatc cctccaaggc ttcatccatc agaagcgatt ctcttggcaa ttctggaact
     2401 cccgcgtatc ccgtgctatc ctcggttata tactcatatt tttgtgcggg ggcttttgca
     2461 catgccccta aaagcaacag ggcgatcaaa ctaaaaatca atattttctt cttcattctc
     2521 ttctcctttt taaagcttcc ttagccttaa gcattaaacg aaaaaactcg aataaaagtt
     2581 cccgctacct gcctaaattc aagcgtctcg cttgtattga tgggatgcct cgttcttcga
     2641 tgcgttttgc cacagcagtc acatcataag gcaccctttt gaacataaaa ctattctctt
     2701 caagatccca aatcacataa gcggctctgg ggtcacggtc gcgcggttgc cccaccgcgc
     2761 cggggttcaa tataaagcgt tcttgcggta tccagcggtc gccagattcc ggtttcagca
     2821 aacgcacctt aaaatctgct gattcgataa aaatcgctgg cgtgtgcgta tgccccacca
     2881 aagaaccctg actctcaaaa tactcaaacg ctgcccaggc gatgccaccc tggtccacgt
     2941 actcccaaat gggatcgcgt ggactgccat gtgccagagt aatcccatca aggagcaact
     3001 tttcaggcaa ttcgcttaga aaatcaagcg agcttttcgt catcagactc ttttgcaaat
     3061 tcaaagcctt gcgcgcttca tggttaaagt gagttgtgct tatgatgtct aatactgccg
     3121 cgtcatgatt acccataatg caggttaaat taggcaattc acgtaacaag ctgacacatt
     3181 cttctgggtc aggcccatag ccaaccacat caccaagaca aatcacgcgg tcaaattcac
     3241 ccgcatcagc caaaaccgcc tcaaaggcgc ctagatttga gtgaatgtca gagattacca
     3301 gagcgcgcat ctcgtttagg gcattttaaa atcgtaatgc cacaaatctc ccgtgatgtt
     3361 ttcaacagta ttcaagccag ttaacgctat ccagctctta cccggtttca aggggaacgg
     3421 attaccttcc aggtcataga aagatatcgg tgactcgtta tcgttcgttt tccaaatggc
     3481 ttcgtaggcc tggccattcc taaagataac cgcgcgtctg cctgcggtgt tgccccagac
     3541 tttgatatca atcaactgtg tttcaatttc tacatgctcc acaaaaagca taatcacgtt
     3601 cgaaaatgcc agttgttctt ctgtatttct gtcaacaagc ggcttcatct cataggtgta
     3661 tgcatcttca atccagcgca aatagtttcc actggcttcg tcatattccc aatcgccctt
     3721 gttgtagtag ttaaaatgga attccacttt actggcaggc ttgccaccgg cagggattgc
     3781 ttcactgaat ttcataccgt ccagatttgg tcgcgtggca gggtcaatcc cagcgttctg
     3841 aaaatattcc gtgatagctg ccgtattggc ataaagccgg gttacatcgc cggaacctcg
     3901 gtcacaaatg cccggacagg tcccttccgg aaacaagcga tcacccataa tgcgataaat
     3961 cttaggcatc accttgaccg ggtcaccacc ggaaaaagca atcaccgcgt tatacagact
     4021 tgcgatttca gggtccacaa tacgaatgga gcgaatcggc cacacataat cgctgttctc
     4081 gccataatac aatggcgcga agcgattgga accaccgcca atccaggttt cgaacacgat
     4141 atccgctgac gataaacctc cgtgcgggcg cccataggct gggaagtttg gcactttaac
     4201 cattatcggc aatcgtttaa gaatgctggg gtcagctact tttaaaccgg ttaatggatt
     4261 gacatcttca gggaagttcg tcggcccaac cgagtagact gctggctcag tgggaagaac
     4321 ttcttgtgtg ggtgtttcgg gtacgggtgt ttcagtagct tccggtgttg gttcttctgt
     4381 tttttctggt tcttcggttc ccttttgttt atccaggctt tgttgggtca tcatttccgc
     4441 aaccagattt gccacttctg tttgctgggc ttcctctgcg gacagcccgg attgtattcc
     4501 gcatgcgctc aataataacg cgcatgataa taagataagt agaaagtttc gtcgcatttt
     4561 catcgctata ctccttatct ttctatttta ccttctattt taaaaagcgt ggttcagctt
     4621 aaccgttgca agccatctac cagtgcccgg atatgctccg cgcgcgtgcc acaacaacct
     4681 ccgatggcac gaatgtcaaa cgcttccaca tagcccagga gcgtttcagc aaacgcttca
     4741 ggtccaacag gataaatcaa cgcgccatcc accttttggg gaatgcccgc gtttgggata
     4801 gctgctatcg gcttagtgct atgctcgctt aaataggcga tgggcgcgcg catttgctca
     4861 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccgcgct tgctggcaat
     4981 aaacgtcagc aatgtcttcg taagtttgct cattcctaac agcatccaca ccggcaagga
     5041 aaacgccact gggacccatc gaacccgcaa caaaacgctg tttgccatcg tggctaaatt
     5101 tgtcgatttc atcacgggcg ataagcgcgg caatggtatt gatttcatca gttttatgcg
     5161 ccaaaccata tttcgccaga gcaattcgat tggcttgaaa ggtgtttgtt tgaagaacat
     5221 ccaccccagc tgccaaaaat tgccggtgaa cttcagcgac aagatttggc gcgctaagca
     5281 caagcgattc atacggactt accatttcca ccatgtcaca gtcggacgtg gtcaataaca
     5341 gagcctgcaa atttgtcgcc atcgcgccat caaagacaag cacacgctct tgaagagcgt
     5401 ccaggtaagc ggttttggtc tttggtactt caggtgtcat ggtcgattct acagaaaaca
     5461 gttttgttat taaaagtcta ccaaagggat tattgatggt caaacaatat tcagttctaa
     5521 atttgttgta ttaaagcgcg gttttcattt ctcctgccgg cgtctcgcca tgcaagattg
     5581 atatcccatt gatattccca cgaacaccac taatctgtca aaacctattg acataagctc
     5641 ccactaccgt ataatatgaa cttatgaaca cctgctcata acttcatgca cgcacatcaa
     5701 cagaaagtgg caaataatat gaaaactgag ataccaaaca agcccataaa ccttctaccc
     5761 cctgacgatg aaaacatcaa gcgtatggcg gaaactttca aaggcctggc agacccaacc
     5821 cgcttaaagt tgattgactt gctcctgaaa ggcgagcgtt gcgtaaacga tttagccgaa
     5881 gcgacccaaa ccagccaaag tgctgtttcg catcagttga gacttttgcg caatttgcgt
     5941 tttgtatctt cccgacgcga tggtcaacag gttttttacc gcatcaacga cgaacacatc
     6001 ggcgaacttt tcaaacttgc attagaccat gccttgcaca tgcacgaaag gatttaatga
     6061 gcgaacacca ccatcatcaa tcaagcagtt gtggctgtag tggcgacacc tgtgggctta
     6121 tggttactcc cgaccccgaa ggcgtaaaac acagcaaaat cttggatttc aaaggctttt
     6181 ggcaacattt ttggcaaatg ccggaactaa aacttgtcat tcccggcatc ctgctcctgg
     6241 cgattagcct gtttctgccc cgatttggcg tgtcacaccg ctggaccatc atcattcaac
     6301 tgctcatctt gcctgttgcc ggctggtttg tgcttagtca tggcttcaag agcctgttca
     6361 tcagacgcaa gctgaacatg gacgtgctga tgtcactggc actgattggc gcggtgttaa
     6421 ttggcgaagg caccgaagcc ttaatcctgc tcattctgtt cacgctttct gaagcaattg
     6481 aaggctacac cagtgccagc gcacgcaaag ttctgaccga atttgccgac ctggcaccca
     6541 aagaagcctt gcgcctgaca aacggcgaag agagcctggt accggttgaa gaattaaaaa
     6601 ttggcgaccg catcatcgtt cgagctggcg accgcctgcc catggatggc at
//
LOCUS       DAOSFC010000042         6648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10052_length_6648_cov_5.266343, whole genome shotgun sequence.
ACCESSION   DAOSFC010000042 DAOSFC010000000
VERSION     DAOSFC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10052_length_6648_cov_5.266343"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PK791_01935"
     CDS             <1..1078
                     /locus_tag="PK791_01935"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH92103.1"
                     /translation="PMTFELIPFLKEMLSVPGLSGYEKPVRDVIARAWEPLSDELSLS
                     NLGSLLALKRGEGPDPRPTAMIAVHMDAIGLMVTAIEDGFLRFTQVGGVDPRILPGQP
                     VIVHATKGKEAQDLPALVVMPPAQLLPKEAGTGPLAQTYLLVDTGLRPKDVEKLVRVG
                     DIISFASQPEELAGDTIAGHTLDNRASVAAATVALELLAKRRHLWDVVVDATVQEEVG
                     CIGGFLDPIKVKPDLAIVVDVNFATGPGGSGSMNFEMGGGLPLNWGANVHPALYKAME
                     KTAETYEIPIYKDFSPSMSGTDAYGIQVAEEGIPVVEISIPLRYMHTPVEQVAIKDIQ
                     RAGRLMAEFITDLPLDFMNTVKWE"
     gene            1081..2172
                     /locus_tag="PK791_01940"
     CDS             1081..2172
                     /locus_tag="PK791_01940"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92104.1"
                     /translation="MAKTLETPKTFKPKMGKKELELLSKLTDALGVSGDEQEIRQIVM
                     DELEGIADEMRVDHLGNLLVTKLGKGEKRLRVMVAAHMDEVGFMLIKDEGKGIFEFRV
                     VGGIDPRQLVGKQVIVGRDHVPGIIGAKAIHLTTPEERKQTLPVTQLRIDLGPGGSAK
                     AKPGDRATFATKLQVMGPTLCAKALDNRLGVYNLIQLVKNAPENIDLLAAFTVQEEVG
                     LRGAKVAAFSFNPDIGIAIDSTPAFDLPHYDGEENTLYRTHLDGGPAIYVMDGATIAD
                     RRLVDWLVDTAKAENIPWQFRQPAPGGTDAGAIHRVREGVPSVSVSVPGRYAHSAAGM
                     ARIDDELNTLRLLHAALARLDENILLRQE"
     gene            2176..>2381
                     /locus_tag="PK791_01945"
     CDS             2176..>2381
                     /locus_tag="PK791_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92105.1"
                     /translation="MTEKKQDTLLQLIKKLVETPGPSGQEQKIRAAVREEIEGYADEI
                     RTDALGNLIARVGSKKEGGMRVMVS"
     assembly_gap    2382..2481
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2482..3017
                     /locus_tag="PK791_01950"
     CDS             <2482..3017
                     /locus_tag="PK791_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH92106.1"
                     /translation="VAKSMDDRIAAVIAIEAMKQIKKTSPNEIYFVFSVQEEVGLRGA
                     TTAAYGVNPEVGIAVDVTGVGDTPKGFKLNVKLGNGPAVKVRDNSFIADPRVVKWMVN
                     EAEAQGLPYQMEVLTFGGTDGRAIQMTREGVPAGCLSIGSRYIHTPSEMVDRDDVENS
                     IKLLAGLLNNKIELGEL"
     gene            3072..4409
                     /locus_tag="PK791_01955"
     CDS             3072..4409
                     /locus_tag="PK791_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92107.1"
                     /translation="MMKKTHSWLKPVTLLFAILVLLVSAGCEAILSIDFPWEEHAGVP
                     TKTPVPVLSTPTATPIPTKVNVEATATQTPRQTRMVIWLPPEFDPEADNEAARLLKAR
                     LENLARKNKIELDVRLKNPIGNSNLIESLIATNGAAPDNLPAVVLLRRQDLDAAYARG
                     LVYNNDELASMAGAIDWYSFARETVSYDGVSYGLGLFGDPMVLVYKAQSELIPGNDWF
                     SMHDNFGYFGFAADDSQGRFLLLLYLAAGGETRDAQNHLVLQEEPLAQALQVLKDLVK
                     TKHLNHTVLEMQTQSAVWQAYKNWQLDTAAMPASIALKALVSEETGYPVLAFNQPDFT
                     LTESWALALTHPDESQQALGLLLMQDLTNTDFLAKWSEALGYVPAKSASLGAWTNVEL
                     KPALEKTMNIALLYPRDETINSLGPILRNATLMILRDNASVEDAVQAALEGLK"
     gene            4436..4825
                     /locus_tag="PK791_01960"
     CDS             4436..4825
                     /locus_tag="PK791_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92108.1"
                     /translation="MDESRRKETELTNEQSPTEYSDESCRKKEPSKGFVAAVIGLAVV
                     TLVLVLIVVITPPIMSPQAKVAATAISITKTIAAKTGAYVDPDLLIEPLPVENVSGIA
                     ILGGLMALIVLGVTLREFFLWRKRKKN"
     gene            4928..6040
                     /locus_tag="PK791_01965"
     CDS             4928..6040
                     /locus_tag="PK791_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOH92109.1"
                     /translation="MQKRLNYLIQNLEKHQLDAVALNPGPSLNHLTGLDFHLMERPVV
                     AVFWADGAVQIILPELERAKLARLPSEVRVSTFGDVIDTWQSVYTQALESASKDSLRI
                     GIEPTRLRYLELNLLQTALPHAKFEDGSACIAPLRLRKDASELAKMKRAAEIAQNALL
                     ETLKTVRPGQSEKQIAAELMVQLYRAGSDPELPFSPIVSTGPNTANPHASPTDRLLGE
                     GEFLLIDWGAGFEHYFSDITRTFGCGEPNAEMRKIAELVRQANEAARLGGKAGMTCGD
                     IDHLARDVITKGGYGERFTHRTGHGLGMEAHEAPWIVGGSPVVLEEGMVFTIEPGIYL
                     EGFAGVRIEDDVVVTTDGLLSLTDLPREVLPLETFC"
     gene            6071..>6648
                     /locus_tag="PK791_01970"
     CDS             6071..>6648
                     /locus_tag="PK791_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH92110.1"
                     /translation="MKLSIKQISLGPLETNAVLILDEENKQAIVVDPSFNPEPLATWA
                     ENEGIEIVQIWITHGHADHTAGVPALMRALPKPADLLMSAEAYAMVQKQGTEFFGFPL
                     EPFPKPTRFIQHGEKLGFGENAEIAEARFAPGHAPGSVVYYLADAGVAIVGDVIFRAG
                     IGRWDFEGGNFKVLTQSIQEQVFTLPDETILLP"
BASE COUNT         1636 a         1644 c         1766 g         1502 t
ORIGIN      
        1 gcctatgacc ttcgaattga ttcccttcct aaaagaaatg ctctctgtac ctgggctcag
       61 cggatatgaa aaacctgtcc gcgatgtaat tgcccgggca tgggagccgc tgagtgatga
      121 attgagtctc agcaatttgg gcagcttgct cgctttgaag cgtggcgagg gccctgatcc
      181 cagaccaacc gccatgattg ccgtgcatat ggacgcaatc ggtttgatgg taaccgccat
      241 tgaagatggc tttttgcgat tcacccaggt gggtggcgtt gatccgcgca ttttgcccgg
      301 acaacccgtt atcgtgcatg caaccaaggg taaggaagcc caggatttac ccgccctggt
      361 ggtgatgcca cccgcgcaac ttttacccaa ggaagccggc acgggacccc tggcacagac
      421 ctatttgctg gttgataccg gcttgcgccc caaagacgtt gaaaaactcg ttcgcgtggg
      481 cgatatcatt tctttcgcct cgcaaccgga agagctggct ggcgatacca tcgccggaca
      541 taccctggac aaccgcgcgt ctgtggctgc ggccacggtc gccctggaac tgcttgccaa
      601 acgtcgccat ctttgggacg tggtggttga tgcaactgtg caggaagaag tgggttgcat
      661 cggtggcttt ttggatccca tcaaagttaa gcccgatttg gcaatcgtgg tggatgtgaa
      721 cttcgcgact ggacccggtg gcagtggcag catgaacttt gaaatgggcg gtggtttacc
      781 cttaaactgg ggcgccaacg tgcatcccgc gctttataaa gccatggaaa aaactgcgga
      841 aacctacgaa atccctatct acaaagactt ttcgccgagc atgtctggca cggatgctta
      901 tggcatccag gtcgctgaag aaggcattcc cgtggttgaa atcagcattc ccttgcgcta
      961 catgcacacg ccggttgaac aagtcgccat caaagatatc caacgggctg gcagactgat
     1021 ggctgaattt atcaccgacc tgccactcga ttttatgaat actgtgaaat gggagtagac
     1081 atggcaaaga cattggaaac cccaaaaact ttcaaaccca aaatgggtaa aaaagaactt
     1141 gaacttctta gcaagctgac cgacgccttg ggcgtttcgg gcgatgaaca ggaaatccgc
     1201 caaattgtga tggacgaact ggaaggcatc gccgatgaaa tgcgcgttga ccatcttggc
     1261 aaccttttgg taaccaaact gggcaaaggc gaaaaacgcc tgcgcgtgat ggttgccgcg
     1321 cacatggacg aagttggctt tatgctgatt aaagacgaag gcaaaggtat ctttgagttt
     1381 cgtgtggtgg gtggcattga cccgcgccag ctggttggca agcaagttat tgttggccgc
     1441 gaccatgtgc cgggcatcat cggtgccaaa gcaatccacc tgaccacgcc cgaagaacgc
     1501 aaacaaactt tgcccgtaac acaactgcgc atagacctcg gcccgggtgg gtctgcaaag
     1561 gctaaacctg gcgaccgcgc cacattcgcg accaagctcc aggtgatggg ccccacactt
     1621 tgtgccaagg cgctggataa ccgtctgggt gtttataacc ttattcagct ggtgaaaaat
     1681 gcccccgaaa acattgacct tttggcggct ttcaccgtgc aggaagaagt cggtttgcgt
     1741 ggcgcgaaag tggcagcctt tagcttcaac cccgatatcg gcatcgcgat tgactcaacc
     1801 cccgcgtttg acttacccca ctacgacggc gaagagaaca ccctgtatcg cacccatctt
     1861 gatggtgggc cggcaattta tgtgatggac ggtgccacaa tcgctgaccg acgtttggtt
     1921 gactggctgg tggataccgc caaagccgaa aatatcccct ggcaattccg ccaacccgcg
     1981 ccgggtggca ccgatgccgg cgcgattcac cgcgtgcgcg aaggtgtgcc gagcgtttcg
     2041 gtttctgttc ccgggcgata tgcccacagc gcggctggaa tggcgcgcat tgatgatgaa
     2101 ctcaacacgt taagattgct tcacgccgca ttggcgcgtt tggacgaaaa tatcctttta
     2161 aggcaggagt aaagaatgac agaaaagaaa caagatacat tactgcaact aattaaaaaa
     2221 ctagttgaaa cccctggccc ttccgggcag gaacaaaaaa tccgcgctgc agtgcgcgaa
     2281 gaaatcgaag gctatgccga cgaaatccgc acggacgctt tgggtaatct catcgcgcgc
     2341 gtgggcagca aaaaagaagg cggtatgcgc gtgatggttt cnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn ntggtggcaa agtcgatgga cgaccgtatc gcggctgtta
     2521 tcgctattga agcgatgaag caaattaaga aaaccagccc gaacgagatc tacttcgtgt
     2581 tcagcgtaca ggaagaagtc ggtcttcgtg gcgcgacaac agccgcttac ggtgtgaacc
     2641 cggaagttgg catcgcggta gacgtgaccg gcgttggcga tacgcccaag ggctttaaac
     2701 tcaacgttaa acttggtaat ggccccgcgg tgaaagtgcg cgacaattcc tttatcgctg
     2761 atccgcgtgt ggttaagtgg atggttaacg aagccgaagc ccagggcttg ccctatcaaa
     2821 tggaagtgct tacctttggt ggcacggacg gacgcgccat ccaaatgacc cgcgaaggcg
     2881 tgccagcagg gtgtctatcc attggcagtc gctatatcca tacccccagc gaaatggttg
     2941 accgtgacga tgtggaaaac tcaatcaaac tgttggccgg cttgctgaac aacaagattg
     3001 agttgggcga actctaaagt tttgctttta aggcaaaaca ctttagcctt aaaggcgatt
     3061 ataataaagg catgatgaaa aaaacgcaca gttggctgaa accggtaaca ctattgtttg
     3121 caatactcgt gttgcttgtt tcagctggct gtgaagcaat tttgagtatc gacttcccct
     3181 gggaagaaca tgccggcgtg cctaccaaaa cgcctgtgcc agtcctttca acgccaactg
     3241 ccacacctat accgaccaaa gttaatgtgg aagctaccgc tacccagaca ccacgacaga
     3301 cgcgcatggt gatttggttg cccccggaat ttgaccccga agcggataac gaggctgccc
     3361 gcttgctaaa agcccgactg gaaaacttag cccgaaaaaa taagattgaa cttgatgtgc
     3421 gccttaaaaa ccccatcggc aacagcaatt tgattgaatc gctgatcgcc acaaatgggg
     3481 ctgcgcctga taacttaccc gctgtcgttt tgcttcgccg gcaagacctt gatgccgcct
     3541 acgcgcgcgg attggtttac aacaacgatg aacttgccag tatggcgggc gcgatagact
     3601 ggtatagctt cgcccgcgaa acagttagtt atgatggcgt aagttatggc ttgggtctgt
     3661 ttggcgaccc gatggttttg gtctataaag cgcaatcgga gttgatacca gggaacgatt
     3721 ggttctcgat gcatgataat ttcgggtatt ttggatttgc tgctgatgac tcgcaaggtc
     3781 gctttttgct tttgctttat ctggccgcgg gtggcgaaac gcgtgacgcg caaaatcatc
     3841 ttgttttaca ggaagaaccc cttgcacaag ctttgcaggt cttaaaagat ttagtcaaaa
     3901 caaagcattt aaatcatact gtgctcgaaa tgcagacgca aagtgcggtt tggcaagcct
     3961 ataaaaactg gcaacttgat acggctgcta tgcctgccag catcgcatta aaagcgctgg
     4021 taagtgaaga aaccggttat ccagttctag cttttaatca gcctgatttt acgctgaccg
     4081 agtcctgggc cttggcactg actcacccag atgaaagcca acaggctttg ggccttttgc
     4141 tgatgcagga tttgaccaac accgattttc tcgccaaatg gagtgaggca ctgggctatg
     4201 tgcctgccaa gtcggcgtca ttgggcgcgt ggaccaacgt ggaacttaaa cctgccctgg
     4261 aaaaaacaat gaatatcgcc ctgctttacc cacgcgacga aaccatcaat agccttggcc
     4321 ccattttgcg caacgcgacc ctgatgattt tgcgcgataa cgcttccgtg gaagacgctg
     4381 ttcaggctgc acttgaaggt ttgaaataag acttgagaga ttggatggga taaatatgga
     4441 tgaatcacga cgaaaagaga ccgaattgac aaatgaacaa agcccgacgg aatattctga
     4501 tgaaagttgc agaaaaaagg aacccagtaa aggctttgtg gccgcggtga ttggtctggc
     4561 agttgtaacc cttgttctcg tcctgattgt ggtcattaca ccgccgatca tgagtccgca
     4621 ggccaaagta gcagcaaccg caatttcgat taccaaaacc atcgcggcaa aaacgggagc
     4681 ctatgttgac ccggatttgc tgattgagcc attgccggtt gagaatgtga gcggcatcgc
     4741 gattttaggt gggctaatgg cactcattgt gctgggtgtg accttgcgcg agtttttcct
     4801 ttggcgaaag cggaaaaaga actaaatttt ctctggtgat tatttaaaaa cgtcgtgaag
     4861 gcgttttttc ttttaagcct gcttgcactt ggtataatcg tagcatcatg aaaaacggac
     4921 ggatagcatg caaaaacgac tcaattacct tatccaaaac ttagaaaaac atcagctaga
     4981 cgcggtagcg ctcaaccccg gaccaagctt aaaccatctc actgggctgg attttcattt
     5041 gatggaacgc ccggtggtgg cggtcttttg ggcagatggc gcggtgcaaa tcattttgcc
     5101 ggaactggag cgcgctaaac ttgccaggct gccttctgaa gtgcgtgtga gcacctttgg
     5161 cgacgtgatt gatacctggc aaagcgttta cacacaagcc ttggaaagcg cgtcaaaaga
     5221 ctccctgcgc atcggcattg agccaacacg cttgcgctat cttgaactga acttattgca
     5281 aaccgccttg ccccacgcaa agtttgaaga tggcagcgcg tgcatcgcgc cattgcgcct
     5341 gcgcaaggac gcttctgaac tggccaaaat gaaacgcgcg gcggaaatcg cccaaaacgc
     5401 tctacttgaa acgcttaaaa ccgtgcgtcc cgggcaaagc gaaaagcaaa tcgctgccga
     5461 actgatggta cagctctatc gcgcgggttc tgaccctgaa ctgcctttct cgccgattgt
     5521 gtctaccggc cccaacacag caaacccaca cgcttcgcca accgaccgcc ttttgggtga
     5581 aggggagttt ctgctgattg actggggcgc gggctttgag cattatttct cggatatcac
     5641 gcgcactttt ggctgtggtg aacctaacgc ggaaatgcgt aaaatcgctg aactggtgcg
     5701 ccaggcaaac gaagccgcgc gcctgggagg caaagccggc atgacttgtg gcgatatcga
     5761 ccacctggcc cgtgatgtca ttaccaaagg tggttatggc gagcgcttta cgcatcgcac
     5821 aggacatgga ctgggcatgg aagcccacga agcgccctgg attgttgggg gcagcccggt
     5881 agtgctggaa gaaggcatgg tctttaccat tgagccaggc atctatttgg aaggctttgc
     5941 cggcgtgcgc attgaggacg atgttgtcgt taccacagat ggactactaa gccttactga
     6001 tttgccacgt gaagtcctgc cacttgagac attttgctaa ggtctgatgc agcagaaagg
     6061 atagaacctc atgaaattaa gcattaaaca gataagcctg ggccctctcg aaaccaacgc
     6121 cgtgcttatt ttggatgaag aaaataaaca agcgattgtc gttgacccaa gtttcaaccc
     6181 cgagccttta gccacctggg ctgaaaacga aggcattgag attgttcaga tttggattac
     6241 acacggacac gccgaccata ccgcgggtgt gccggcactc atgcgcgctt tacccaaacc
     6301 agctgacttg ctgatgagcg cagaagccta cgccatggta caaaaacagg ggacggagtt
     6361 tttcggcttt cctttggagc ccttcccaaa gccgacgcga tttattcaac atggtgagaa
     6421 gctgggtttt ggcgaaaacg ctgaaatagc tgaagcacgt ttcgcgcccg ggcacgcgcc
     6481 gggttcggtg gtgtattatt tggcggatgc gggtgtcgca attgtgggcg atgtcatttt
     6541 cagggcaggt atcggtcgct gggactttga aggcgggaat tttaaggtgc tgacgcaatc
     6601 cattcaggag caagttttta ccctgcccga tgaaaccatc ctgctgcc
//
LOCUS       DAOSFC010000043         6589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10179_length_6589_cov_5.297674, whole genome shotgun sequence.
ACCESSION   DAOSFC010000043 DAOSFC010000000
VERSION     DAOSFC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10179_length_6589_cov_5.297674"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..783)
                     /locus_tag="PK791_01975"
     CDS             complement(157..783)
                     /locus_tag="PK791_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH92111.1"
                     /translation="MPKKILLIILALGVSLLCLNFFAPGRASFDVPAIQVLHSVQASN
                     LQQQGQEIPTPVIAATLPVELYKYTATPGPDGYIKHTVLEAQFLFHIAELYGVDLQEI
                     LALNNLTVDSIINPGDILIIKQVEAPTPEPSPMLSELQSTDTEPEETPLPFEPTPELT
                     PTNTVAPESSQGLIERVFTNNSRYLAYAIFALVLLGVVLLIVSSRRIH"
     gene            900..1631
                     /locus_tag="PK791_01980"
     CDS             900..1631
                     /locus_tag="PK791_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92112.1"
                     /translation="MRKIYALVLSVSLLAGLLACVPGEVNPQESFDPVEVTLQALDAT
                     ATAQHTQELPSPEPSATPTVIILSPTTLPTATESPTPKPTTIVFQGFLEQFRLYRAWW
                     LDGKTYFYFLNAGIDFTMFATADDFDLVCEQDPANLTGMICVAESKIEEDEMDFVFYL
                     DQEHEIVVHEASFNAQLMDNIVYHHQFDCPDRGKNVSCESEYRLYDGRCYYAHTCYDA
                     CGLYYSRDNLPKVYREFQGFTGPCN"
     gene            1732..>2020
                     /locus_tag="PK791_01985"
     CDS             1732..>2020
                     /locus_tag="PK791_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92113.1"
                     /translation="MLRKKYYKLALLLLFLSLIACTPKVDYGATYTAFWENLPTDTPF
                     PTDTATPTPTNTSTATPSPSATPTQTDTPVPTETLTPTWMPHPGYYWPEAAF"
     assembly_gap    2021..2030
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2031..2225
                     /locus_tag="PK791_01990"
     CDS             <2031..2225
                     /locus_tag="PK791_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92114.1"
                     /translation="VWWGGDAWCPSRGQNVSCETEYRQYANGCFVGMTCFDACGKFYG
                     VNTIKYGIGDYIFVGSCGTD"
     gene            2236..2706
                     /locus_tag="PK791_01995"
     CDS             2236..2706
                     /locus_tag="PK791_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92115.1"
                     /translation="MKSKFLLILILLMLVSACTPKVDYGATYTAFWTALPSDTPIPSD
                     TPTREPTATLTITPSPTDNITETPTETPTETVTPTVALTARLIWPRAKFGPEHVTWRT
                     SSCPTEGNRLSCEFEYRKDDFGCYVGGTCYDDCGWFYSINTIPPGVTEFSSPCW"
     gene            complement(2773..4113)
                     /locus_tag="PK791_02000"
     CDS             complement(2773..4113)
                     /locus_tag="PK791_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOH92116.1"
                     /translation="MSQITHIIKRDGSQVKFTPERITNAIYRASVAVGNRDREAADEV
                     SRQVVIALEEQLQPGEIPTVEKVQDLVEQKLIENNYATVAKAYILYRNERARQRAKRS
                     DRDSMPSGNIPWHKLWQSLDWAVSHEVHTTRHLNEKIRSGKFHEVVTAAEAAYFEDVE
                     NAANLILQRQDEIRMVFVVGPSSSGKTTTTTIIGEHLARQGFKLKTLNIDHYFFDLEL
                     HPKDEFGDYDFETPQALNLALINQHLVELFAGKEVRIPYYDFKTGKSHPDHTPMHLEE
                     HEIVLIDSLHGLHPEMTADIDDSLKFKLYIEPLLQLKDSQGHYARWTDIRLMRRMLRD
                     ASFRAYDPLQTLLHWHYVRSSELRHIIPYSTTADTVVNSGMPYELPLYKAKLGANFAE
                     WAESLKNDPLRVDAWERATRVNALFQEIDAVEDDSIVPENSVTREFIGGGIYKY"
     gene            4373..4930
                     /gene="frr"
                     /locus_tag="PK791_02005"
     CDS             4373..4930
                     /gene="frr"
                     /locus_tag="PK791_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOH92117.1"
                     /translation="MVDDLLREAKSRMQSALDVLEEDLLGIRTGRASPALVEKLQIEY
                     YGSNVSLIQLATISVPEPRQLLIRPFDGSSLKMIERAIQASDLGLTPNNDGKVIRLNL
                     PPLTEERRRDLVKLVNNRLEEARVAVRNVRRDVLKDLRDFEKEKLISEDELKRAEEEF
                     NKVTNEYVDEVSVIGERKEKEIMEF"
     gene            4934..5653
                     /locus_tag="PK791_02010"
     CDS             4934..5653
                     /locus_tag="PK791_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOH92118.1"
                     /translation="MVKIEPVKGVPSHVGIIMDGNGRWAKARGLPRLLGHRAGTKNLR
                     RVIRAAADAGVKYITFYAFSTENWKRPAEEVNGLMDLLAEFIDDEVRDLHKENARLLH
                     IGHLDGLTPLLKKKVEDAIELTKNNTRITVIIAFNYGGRDEIVTAVRRLIEAGIRPDE
                     VSEERISNALFTAGIPDPDLIIRTSGEKRTSNFLIWQAAYAEWSFPEIYWPDFDEKAL
                     LAEFENYASRERRFGKISEQL"
     gene            5662..6471
                     /locus_tag="PK791_02015"
     CDS             5662..6471
                     /locus_tag="PK791_02015"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOH92119.1"
                     /translation="MFKQRLISSLIFVPISLAAFYFGNWALLLFMGLVLGLGAYEYSR
                     ILNQAGLRVNQVLVVGGVLALVALRHFFGFQYGALSVSVLLLGLAGHALFQYERGNDQ
                     AYQQFALELSGVLYLGWLGAYSISLRHLPNGAWLAVAAVALVMLVDLGAYVVGSLLGK
                     HHFAPRLSPSKTWEGLAGGLVFGAGCGALIGWLGAPVLPELGVWGGLGLGALMAVISP
                     FGDIFLSMLKRGAGVKDAGKLLPGIGGIMDRIDSWIWGMTAAYYFVLLLIR"
BASE COUNT         1586 a         1435 c         1759 g         1799 t
ORIGIN      
        1 ataaaaaagc taagacatac cgccaaagga aagttgattg aaaatgaaaa caatgtcctg
       61 aggcaaattt tggcacaaaa tccccaaaaa gttgacgaaa caaaagacga gcctgagttg
      121 gccgattgag tggatttgta tcgaaacgat ttgtgcttaa tgaatgcgac gcgaactgac
      181 gattaagagc acaactccca acaaaaccag agcaaaaatg gcataagcca gatagcgcga
      241 gttgtttgtg aacacgcgtt caatcagtcc ttggctggat tcgggagcga cggtgttggt
      301 gggtgtgagt tcaggggttg gttcaaaggg cagtggtgtt tcttccggct cggtgtcagt
      361 gctttgaagt tcagaaagca tcgggctggg ttctggtgtg ggtgcttcga cttgcttgat
      421 aatcaaaatg tcgccggggt tgatgatgct gtctacagtg agattgttca atgccaaaat
      481 ttcttgcagg tcaacaccgt aaagttcggc aatgtgaaaa aggaactgcg cttcaagcac
      541 ggtgtgtttg atgtatccat caggtccagg tgtggcggta tatttataaa gttcaacggg
      601 cagggttgct gcgataacgg gtgtgggaat ttcctgtccc tgttgttgca gattgctggc
      661 ctgaacggag tgcagaacct gaatcgcggg cacatcaaaa gaagccctgc caggcgcaaa
      721 gaaattaagg cacaataaac taaccccaag cgcgagtatt ataagtagta ttttttttgg
      781 catagtcaac ttgcgacttc ttcttcaaga catattatag ctgaaaaatt tgtttagcat
      841 ttttattcaa gcatccgcac agaaaaattg cgatataatg gcagcgaaag ttatttgcca
      901 tgcgaaaaat atacgcttta gttttatctg tcagcctgtt ggctggtttg ttggcttgtg
      961 tgcctggtga agttaatcca caggaaagct ttgatcctgt tgaagtgaca cttcaagctt
     1021 tggatgccac cgcgactgcg cagcatacgc aagaactgcc gagcccagag ccatcggcca
     1081 cgccaacagt aatcattctt tcacccacaa ccctgcctac agcgacggaa tctcccacgc
     1141 cgaagccaac aacgatagtg ttccaggggt ttttggagca attcagactg tatcgggcat
     1201 ggtggctgga tggcaaaacc tatttttact ttctgaacgc gggcattgat tttacgatgt
     1261 ttgctaccgc ggacgatttt gacctggtgt gtgaacagga tcccgctaac ttaacgggga
     1321 tgatctgtgt ggctgaaagt aaaattgaag aagacgagat ggattttgtt ttctaccttg
     1381 accaggagca tgaaatcgtg gtgcatgaag ccagcttcaa cgcgcaactg atggataaca
     1441 ttgtttatca tcatcaattt gattgcccgg accgcgggaa aaatgtctcc tgcgaatcag
     1501 aatacaggtt gtatgatggg cgttgttatt acgcgcatac ctgttatgac gcctgtggtt
     1561 tgtattattc cagagataat cttccaaaag tctaccggga gtttcagggc tttactggcc
     1621 cctgtaatta gctgggcggt cttgcgactt gcagatatcc cgacggtgtt gatacaatgg
     1681 tggtcggcac agtccgtgat accttgttgt gtgaatgatt gaggcttttt tatgctgaga
     1741 aagaaatact ataaacttgc tttactgttg ctattcttat ccctgatcgc ctgtacgcca
     1801 aaagtagatt atggcgcgac ctatacggct ttttgggaaa atttaccgac cgatacgcct
     1861 ttcccaactg atacagcaac cccaacgccg acaaatacca gcaccgcaac gccaagtcca
     1921 agcgctacgc caacccaaac tgacacaccc gtacccacag aaacactcac acccacgtgg
     1981 atgccacacc caggctatta ctggccagag gcggcgttca nnnnnnnnnn gtctggtggg
     2041 gtggcgacgc ctggtgtcct tcacgaggac aaaatgtgag ttgcgaaacg gaatatcgtc
     2101 agtacgctaa cggttgcttc gtgggtatga cttgttttga tgcctgtggc aagttttatg
     2161 gtgtgaatac cataaagtat ggcatcggtg attatatctt tgttgggtca tgtggtactg
     2221 attgagagcg gtcatatgaa gtcaaagttt ctgcttattc tcattttact gatgcttgtt
     2281 tctgcctgta cgccaaaagt ggattatggc gcgacctata cggccttttg gacagcattg
     2341 ccaagcgata cacccattcc cagtgataca ccaacccggg aacctacggc gacattaaca
     2401 atcacgccaa gtccaactga taacataaca gaaacaccaa ctgaaacgcc aaccgaaaca
     2461 gttacaccaa cggtggcact aactgcgcgc ttaatctggc cacgcgccaa atttggaccg
     2521 gaacacgtta cctggcgcac aagttcatgc cccacggaag gaaacaggct ttcctgcgag
     2581 ttcgagtatc gtaaggacga ttttggctgt tatgtgggcg gtacttgtta tgatgattgc
     2641 ggttggtttt actcgattaa cactattccg ccaggcgtaa ctgaattttc cagtccatgt
     2701 tggtagctaa gctattaaaa caaagaagac cgggcacagc tagtccggtc ttcttttctt
     2761 aaaccttcat ttttaatact tgtaaatccc accgccgata aactcgcggg tgacggagtt
     2821 ttcgggcacg atgctgtcgt cttccacagc gtcaatttcc tggaaaagcg cattgaccct
     2881 tgtggcacgt tcccaggcgt ccacacgcaa ggggtcattc tttagacttt cagcccactc
     2941 ggcaaagtta gcgcccagtt tggctttgta gaggggaagt tcatagggca tgcctgagtt
     3001 tacaacggtg tcggcggtcg tgctgtaggg gatgatgtgc cttagttcgc tggagcgcac
     3061 gtaatgccag tggagcaagg tttgcagggg gtcataagcc ctaaagctgg catcgcgcaa
     3121 cattcgacgc ataaggcgaa tatccgtcca gcgcgcgtag tgcccctggc tgtctttgag
     3181 ttgcagcagg ggttcgatgt agagtttgaa tttgagtgaa tcgtcgatat ccgcggtcat
     3241 ttcggggtgc aggccatgca agctgtcaat caaaacgatt tcgtgttctt caaggtgcat
     3301 aggggtgtga tcagggtggc ttttgccagt tttgaagtcg taataaggga tgcgcacttc
     3361 ttttccagca aagagctcaa ccaggtgctg attgatgagt gccaggttga gggcttgggg
     3421 ggtctcaaaa tcgtaatcgc caaattcatc tttaggatgc aattcaaggt caaagaagta
     3481 gtggtcgatg ttgagtgttt tcagtttgaa gccctggcgc gcgagatgtt caccgatgat
     3541 ggtcgtggtg gtggttttac cactggaact ggggccaacc acaaaaacca tgcgaatttc
     3601 gtcctggcgt tggagaatga gattagccgc gttttcaaca tcttcaaaat aggcggcttc
     3661 ggcagcggta accacttcgt ggaattttcc gctgcggatt ttttcgttga gatgacgggt
     3721 tgtatggact tcgtgagaca cagcccagtc cagcgattgc cataatttgt gccagggaat
     3781 gttaccggag ggcatactgt cgcggtcgga acgcttggca cgctggcggg cgcgttcgtt
     3841 gcggtaaagg atataggctt tggcaacggt cgcgtaattg ttttcaatta gtttctgttc
     3901 cacaaggtct tgcacttttt caacggtggg gatttcgcca ggctgcagtt gttcttccag
     3961 cgcgataacg acctgacgcg agacttcgtc tgctgcttca cgatcgcgat tgccaacggc
     4021 aactgaggca cgataaatgg cattagtgat gcgttctggt gtgaacttca cctgactgcc
     4081 gtcgcgtttg atgatgtggg taatttgact catgctaaac tcctttttgc gcatttcaat
     4141 acgggtggca ttcgtttaag atatctaatc tattaacaag tttgtatact ggcaagtatt
     4201 gtcattctta taatacggtg ttgaacacct tgcgaacgcc gtcgtactgc aaattgctga
     4261 catttataag caatttggct tctttacaag tatactaaag ccgttgcact cgcgcaattg
     4321 tcgcgtatct tgtaaaatag agtatcaaac tgaactaacg aaggaggttc ttatggtaga
     4381 tgatttatta agagaagcca agtcgcgtat gcaaagcgct ttggacgttt tggaagaaga
     4441 ccttttgggc atccgcaccg ggcgcgcgtc acccgctttg gttgaaaaat tgcaaattga
     4501 gtattatggc agcaatgtat ctctcattca gttagctacg attagcgtgc ctgaaccccg
     4561 tcagctgctc attcgccctt ttgatggctc ttcgctgaaa atgattgagc gcgctattca
     4621 ggcatcagat ttgggcttga cgccaaacaa tgatggcaag gtgattcgcc tgaacctgcc
     4681 cccgcttact gaggaacgtc gccgagattt ggtgaagctg gtcaacaacc gtcttgaaga
     4741 agcgcgtgtg gcagtgcgca atgtgcgtcg cgatgtgttg aaagacttgc gtgacttcga
     4801 aaaagaaaaa ttgatttctg aagatgaact aaaacgcgct gaagaagaat tcaataaagt
     4861 gaccaacgaa tatgttgacg aagttagcgt aatcggcgaa cgcaaagaaa aagaaataat
     4921 ggagttctag ccgatggtaa aaatcgagcc agttaaaggc gtgccaagcc acgtcggtat
     4981 catcatggat ggaaatggcc gttgggcaaa ggcgcggggc ttgccacgtc ttttagggca
     5041 tcgtgccggc accaagaact tacggcgggt cattcgtgcc gcggcggatg ccggcgtgaa
     5101 atatatcacc ttttacgctt tctcaaccga gaactggaaa cgcccagccg aagaagtgaa
     5161 tggcctgatg gatttactgg ctgagtttat cgatgatgag gtgcgcgatt tgcacaagga
     5221 aaacgcgcgc cttttgcata tcgggcattt ggatggttta acaccgcttt tgaaaaagaa
     5281 ggtggaagac gccattgagc tgaccaaaaa taacacgcgc atcacggtga ttattgcatt
     5341 caactatggc ggacgtgacg agattgtgac tgctgtgcgt cgtttaattg aagcgggtat
     5401 ccgtccagat gaagtgagcg aagagcgcat aagcaatgcc cttttcaccg cgggtattcc
     5461 tgaccctgac ttgattatcc gtacgtcggg agagaaacgc acgagtaact tcctgatttg
     5521 gcaggcggcc tatgccgagt ggtctttccc cgaaatttac tggcccgact ttgacgaaaa
     5581 agcccttttg gcagagttcg aaaattatgc cagtcgtgaa cgccgttttg gcaagataag
     5641 cgaacaactc taagttcatt tatgtttaaa caacgtctga ttagctcgct tatttttgtg
     5701 cccatatccc tggcggcttt ttattttggc aactgggcac ttttgctgtt catgggcctg
     5761 gttttgggac tgggcgcgta tgaatattcg cgcattctca accaggcagg tttgcgggtg
     5821 aatcaagttt tggtagtcgg tggcgttttg gcgttggtcg ctttacgcca cttttttggt
     5881 tttcaatatg gggcgttgag tgtgagcgtt ttgttactcg ggcttgctgg gcacgcgctt
     5941 tttcaatatg agcgtggcaa cgaccaggcc tatcagcaat ttgctttgga attgagtggg
     6001 gtcttgtatt tgggttggct gggcgcatat tcaatttcct tgcgtcactt gcctaatggt
     6061 gcctggttag ctgtggcagc agttgctttg gtgatgttgg tcgatttagg tgcctatgtc
     6121 gtaggctctt tgcttggcaa gcatcatttc gcgccaagat tgagtccttc gaagacctgg
     6181 gaagggctcg cgggtgggct cgtgtttggc gcgggctgtg gcgcgctgat tggctggctg
     6241 ggggcacctg tgttgccgga actgggtgta tggggcggac ttggcctggg ggccttgatg
     6301 gctgtcatct cgccttttgg ggatatcttt ttgagtatgc tcaaacgtgg cgcgggcgta
     6361 aaagatgctg gcaaacttct gcctggcatc gggggcatta tggaccgcat cgactcctgg
     6421 atttggggca tgaccgcggc gtattatttt gtcctgttgc tgattagata gccgtttttt
     6481 gttttaagac agatttagag ccgaatgtat ctcgcgtttc ttcccatagc taagcgagtt
     6541 attttcccat ccctaaccat ttcaccgagt acggcttcga ttgttgatg
//
LOCUS       DAOSFC010000044         6547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10280_length_6547_cov_5.283888, whole genome shotgun sequence.
ACCESSION   DAOSFC010000044 DAOSFC010000000
VERSION     DAOSFC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10280_length_6547_cov_5.283888"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PK791_02020"
     CDS             <1..283
                     /locus_tag="PK791_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOH92120.1"
                     /translation="KLCLIGDVKPGASLSADESILIWGKASGQITAGEKPCPNNCIFV
                     LFYDGAQFRLNGKEVALPKKLKTGVTLKIWQEDQTIMVEVLKEKRFGIL"
     gene            280..1404
                     /locus_tag="PK791_02025"
     CDS             280..1404
                     /locus_tag="PK791_02025"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOH92121.1"
                     /translation="MNTRDIWRRFDIPLFAAVLLLCIFGIALISSAIAGNPTLEGHPK
                     RQAIFMLIGLVALFASAAFDYRIWQTISKPFYIFVLVLLLVVFIMGAARFGAARWLET
                     GLVTLQPAELAKIAIILSLSSFFAANISNINNWATIGKSIMLTGGIVLWIMLQPNLST
                     SIVILVIWATMLWICGLETKYILYAVLFFAVFIGLSFAFDFPYLADYQKSRITNFLSP
                     DENARYGDTYNVDQAKISIGSGGWFGNGFGQGSQVQLRFLKIRHNDFIFSVLAHEFGF
                     VGTVIVLALLVFIIVRCFMIAKEAPDAYGSLIAYGFGILMAFQTMVNVGVNLNVLPVT
                     GLTLPFISYGGSSLVSMLLGIGIVQSIRMRSSPKEKGIAN"
     gene            1593..2294
                     /locus_tag="PK791_02030"
     CDS             1593..2294
                     /locus_tag="PK791_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92122.1"
                     /translation="MSESFNLFRLQQLDLNRMKIRRRQREIEKILNADESVQKAQQML
                     DATHQELEKATHAYNEIHDQVEERNLKLKYSQAKLFGGQITNPKELRDLEAESAALKS
                     YLSELAEQQFYALEALEKCQNKLAKAEANLAQVKSASATKHSKLMGESRQLEEKLPAI
                     NEQRESLRAQISPANYQAYVKLLKSKGGRAVAEVIDSSCNACGVNVPPGDIQRAKSPS
                     EIAYCRTCGRMLYAK"
     gene            complement(2295..2555)
                     /locus_tag="PK791_02035"
     CDS             complement(2295..2555)
                     /locus_tag="PK791_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOH92123.1"
                     /translation="MKGVITAVNILGQLLAVLVVLYVIIQMIFPNSERRGVLARLLDP
                     ILAPIRKRVKPYKGFDFAPLILLVLILLVEIVVVAVLGRFVK"
     gene            complement(2564..3295)
                     /locus_tag="PK791_02040"
     CDS             complement(2564..3295)
                     /locus_tag="PK791_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOH92124.1"
                     /translation="MSELDIVEAMQSRYTQVMARVADATRRSGRKPQDVLVLAVTKLQ
                     SVEKIRAVYSAGIRAFGESYVEEALPKIETLNDLSEARWEMVGHVQSRKAKLVANAFS
                     RIHSLGTQKLARLLDRHREPGNGPLDVLMQVNLSGEDSKQGIVATKRETWRDVLAFAQ
                     ELKAYPNLRLTGLMTMPPLFDDPEHNRPYFRQLRELRDYLNEQDPSLNLTELSMGTSY
                     DFEIAVEEGATIVRLGSILLGERQY"
     gene            complement(3298..4089)
                     /gene="pgeF"
                     /locus_tag="PK791_02045"
     CDS             complement(3298..4089)
                     /gene="pgeF"
                     /locus_tag="PK791_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOH92125.1"
                     /translation="MKTIDVNGTRAYQFESFDANAVDHGIYTRLGGVSEGIWKSLNTA
                     GTVGDDKTAIAQNHEILFGAFGRPLATRFDVWQVHGTQIHFAEAPRPPEQKHMPGDAI
                     FTDKAGVTLMMRFADCVPLLFHDPVQKVIGIVHAGWLGTALQIGRVAVEALAERYGSK
                     PQDLVVGIGPAICGDCYEVGEDVVRRFEKYWKENYQQFFKQKDQSLYLDMLGANEYVL
                     RKAGVTQIEQSGICTAENLHEWYSYRKEKGLTGRFAVLLALKDEA"
     gene            complement(4076..4816)
                     /locus_tag="PK791_02050"
     CDS             complement(4076..4816)
                     /locus_tag="PK791_02050"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOH92126.1"
                     /translation="MAKFARKRLAKRRSKKDRDRLQRWLLVLVAILLVVYLGGAVLAP
                     VLMKAGHTTQARRIYKAYQPACHQLAYRSFFLYGEQAVYPRDLAGVKGLKSYEAVTGL
                     SAEDIESAKNFIGNEQLGYKTALCQRDLAIFGSILLFIIVYAIFEKRIKPLPWWLWIL
                     LAVVPIGLDGFWQVLSQLDIAFLAWLPARESTPFLRVLTGALFGIFSAWFIILSFRGE
                     EDPALYATEIIPDRSNLAEEAEQSDEND"
     gene            complement(4817..5902)
                     /locus_tag="PK791_02055"
     CDS             complement(4817..5902)
                     /locus_tag="PK791_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOH92127.1"
                     /translation="MKNWKISRREFLKMSGMVMGALALPKLEPRTQAVEQFIDGKLYG
                     RIAVGDVGAWTEMRSEPNVDAPVVKTIYRDDVFEIKRELVANKLDPNRYKQRWYETPE
                     GYVHSWMMQPCRSYYNPVVNALPEQVDGQHGMWVEITIPKVDIQVQGSKENSSYWIRS
                     VPQPRLYYSQVHWVSDIRQHNGRTEYLLSEKYGALPDQYWVDARACRVITPEEIAPIH
                     PEAGEKRIVVDLYNQTLYAFEGKREVYFCEVSTGYVKEGKWTTTPGVHHTWRKMISLH
                     MSAGGVSQYDSPGIAWTLLYHSDGQAIHTAYWHNNFGVALSHGCVNCLPHDAKWIWRW
                     SEPYVSYYPGEQTIQGFVGNTTVEVIE"
     gene            complement(5986..6435)
                     /gene="smpB"
                     /locus_tag="PK791_02060"
     CDS             complement(5986..6435)
                     /gene="smpB"
                     /locus_tag="PK791_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOH92128.1"
                     /translation="MNVKVVSTNRKARFEYFVSDTYEAGLVLKGTEIKSIRQGKMSLQ
                     EAYVRTDGEEAWLVGAHIAPYEQASTAQHNPTRDRKLLLHKKEIRQLWNAVRIKGMTI
                     VPLRVYLKAGRAKLEIGVARGKKQYDKRESIKERDIQRESSRRGGDF"
BASE COUNT         1726 a         1813 c         1409 g         1599 t
ORIGIN      
        1 caaactctgt cttatcggtg acgtaaaacc aggcgcaagc ctgagtgccg atgaatccat
       61 tctcatttgg ggcaaagcgt ctggacaaat aactgcaggt gaaaaaccct gccccaacaa
      121 ctgcattttc gtcttgtttt atgatggcgc gcagttccgc ttgaacggta aagaagtagc
      181 cctgcccaaa aaattaaaaa ctggtgtcac gctcaaaatt tggcaagaag accaaaccat
      241 catggttgag gttcttaaag agaaacggtt tggcatctta tgaacacccg agacatctgg
      301 cgacgctttg atatccccct gttcgcggca gtactcttgc tgtgtatttt cggcatcgcg
      361 ctcatcagtt cagcaatcgc gggcaacccc acacttgaag gacaccccaa acgccaggct
      421 atattcatgc tcatcggttt ggtcgcgctt ttcgcctccg cagctttcga ctatcgcatt
      481 tggcaaacca tcagcaaacc cttctatatc tttgtgctcg ttcttttact cgttgtattc
      541 atcatgggcg cggcccgttt tggcgcagcg cgctggctcg aaacgggtct ggttaccctg
      601 caaccagcag aactggcaaa aatcgcgatc attttgagcc tgtccagttt ctttgcggcg
      661 aacatctcta atatcaacaa ctgggctact atcggcaaaa gcattatgct cacgggtggt
      721 atcgtgcttt ggattatgct ccaacccaac ttgagtacca gcatcgttat tcttgtcata
      781 tgggcgacta tgctctggat ttgcgggctg gaaaccaaat acatccttta cgcggtcttg
      841 ttttttgcgg ttttcattgg tttaagcttc gcctttgact ttccctacct ggcagactac
      901 caaaaatcac gcatcacaaa ctttttaagc cccgatgaaa acgcccgcta tggtgatacc
      961 tacaatgtag accaggctaa aattagcatc ggtagcgggg gttggtttgg caatggcttc
     1021 ggacaaggtt cccaggtaca actccgtttc ctaaaaatcc gccacaacga ctttatcttc
     1081 tccgtgcttg cccacgaatt tggctttgtc ggtacagtga ttgtgctggc gctcctggtt
     1141 ttcatcatcg ttcgctgttt tatgattgcc aaagaagccc ctgatgccta cggctcgctt
     1201 atcgcctacg gttttggtat tttgatggct ttccagacca tggtcaatgt tggcgtcaat
     1261 ctcaacgtgc tgccggtcac aggtctcacc ctgcccttca tcagctatgg gggcagttcg
     1321 ctggtttcaa tgcttttagg catcggcatt gtgcaaagca ttcgcatgcg cagttcacca
     1381 aaagaaaaag gcattgctaa ttaaaactta gaattaagac gaaatatctt gcacgtggtc
     1441 aggagaccac gtgcgaactc gcattattaa gtagatgttt aatgttcgga tatggtctcc
     1501 tgaccatatt cttgtatgga taggtttttt gcgttatcgc cctccacagt gtagaatatt
     1561 attaagacac ttttacagct gattggaggc atatgagcga atcttttaac ctattccgat
     1621 tgcaacaact tgacctaaac cgtatgaaga ttcgacgacg ccaacgggaa attgagaaaa
     1681 ttctcaacgc cgacgaatcc gtccaaaaag ctcagcaaat gctggatgca acccatcagg
     1741 agctggaaaa agccacccac gcttacaatg aaatccacga ccaggttgaa gaacgcaacc
     1801 taaaactcaa atattcccag gcaaaactgt ttggcgggca aatcaccaac cccaaagaac
     1861 tgcgggatct ggaagcagaa agtgcggcac tcaaaagcta cctctccgaa ctggccgagc
     1921 aacaattcta cgcgcttgaa gcccttgaaa aatgccaaaa taaacttgcg aaagcagaag
     1981 caaacctggc acaagtcaaa agcgcgtcag ctacaaaaca ttcaaaactg atgggcgaaa
     2041 gccgccagct agaagaaaaa ctgcccgcca tcaacgaaca gcgcgagagt ttacgcgctc
     2101 aaatatcacc ggccaattat caagcctatg taaaactttt gaagagcaaa ggcggacgtg
     2161 ctgtggcgga agtgattgac tcaagttgta atgcctgtgg tgtcaatgtg ccccctggcg
     2221 atatccagcg cgccaaatct ccctcagaaa tcgcctactg cagaacctgt ggcagaatgc
     2281 tttacgccaa ataactactt tacaaaccgc cctaaaacgg cgacgacaac aatctcaaca
     2341 agcagaatca aaaccagcaa aatgagtgga gcgaagtcaa atcccttata gggcttcact
     2401 ctttttcgga taggggctaa gatcgggtcc agcaagcgcg ccaaaacccc acggcgttct
     2461 gagtttggaa aaatcatctg aatgataacg tacaaaacaa ccaaaaccgc caataactga
     2521 cccaggatat taaccgctgt aataacacct ttcatctcgc tccttaatac tgtctttccc
     2581 caagtaaaat actgcccaag cgcacgatgg tcgccccttc ttccaccgcg atttcaaaat
     2641 cataactggt acccatgctg agttccgtga gattaagcga tgggtcttgc tcattcaaat
     2701 aatcacgtaa ctcgcgcaac tgccggaagt aaggccggtt atgttcgggg tcatcaaaca
     2761 aaggcggcat tgtcatcagc ccagtcaacc gcaaatttgg atatgctttc agttcctgag
     2821 caaacgccaa aacatcccgc caggtctcac gtttggttgc cacaatgcct tgtttggaat
     2881 cttcgccact caaattcacc tgcatcagca cgtcaagtgg cccattacca ggctcacgat
     2941 ggcgatccaa aagacgcgcc agtttttgcg tgcccaggga atgtatccgc gaaaaagcat
     3001 tcgccacaag cttagccttg cggctttgca catgcccaac catctcccat cgcgcttcac
     3061 tcaaatcatt cagcgtctca atttttggca aagcttcttc aacatagctt tcgccaaacg
     3121 cgcgaatgcc ggcggaataa accgcgcgaa tcttttcaac actttgcagc ttagtcaccg
     3181 ccaggaccaa aacatcctgc ggtttgcgtc cgctacgccg cgtcgcgtct gcaacacgtg
     3241 ccatcacttg ggtataacga ctttgcatag cttcaacaat atcgagttca gacatgatta
     3301 tgcctcatcc ttcaatgcca gcaaaaccgc aaagcgaccg gtcagcccct tttccttgcg
     3361 gtaggaatac cattcatgca agttttctgc ggtgcaaatg cccgattgct caatctgcgt
     3421 cacaccggct tttcgcaaaa cgtattcgtt ggcacccagc atatccaaat acaaactctg
     3481 gtctttttgc ttaaagaatt gctgatagtt ctctttccag tacttttcaa agcgcctgac
     3541 cacgtcttcg ccaacttcgt aacaatcgcc acaaattgcc ggcccgatgc caaccacaag
     3601 gtcttgcggt ttcgagccat agcgttcggc aagcgcttcc actgcaaccc ggccaatctg
     3661 caaagcggta cccagccaac ccgcgtgaac aatgccaatc actttctgaa ccgggtcatg
     3721 aaaaagcaaa ggaacacaat ccgcaaagcg catcatcaag gtgacgccag ctttatccgt
     3781 aaaaatggca tcgccaggca tatgtttttg ctcaggcgga cgtggtgctt ccgcgaaatg
     3841 gatttgagtg ccatgcacct gccacacgtc aaaacgcgtt gccaagggtc gcccaaaagc
     3901 gccaaagaga atctcatggt tttgcgcaat cgcggtttta tcgtccccca ccgtgccagc
     3961 ggtattcagg ctcttccaaa tcccctcgga tacaccaccc aaacgcgtgt aaatgccatg
     4021 gtccacagca ttcgcgtcaa aactttcgaa ctgataagcg cgcgtgccgt tgacatcaat
     4081 cgttttcatc gctttgctct gcctcctctg ctaaatttga acgatccgga atgatttcgg
     4141 tggcatataa tgcgggatct tcttccccgc ggaatgacaa gataataaac cacgcgctaa
     4201 aaatgccaaa taaagcaccc gtcagcaccc gcagaaaagg ggtactctcg cgggctggga
     4261 gccacgccaa aaaggcgata tccaactggc tcaagacctg ccaaaagcca tccaaaccga
     4321 tgggcacaac tgccagcaaa atccaaagcc accagggaag cggtttgatt ctcttttcaa
     4381 aaatcgcata gacgatgatg aacaaaagaa tcgatccaaa aatcgctaaa tctcgctgac
     4441 aaagcgcggt tttataaccc agttgttcat taccgatgaa atttttggcg ctttcaatgt
     4501 cttccgcgct aagacccgtc accgcttcat agcttttcaa gcccttcaca cccgccaggt
     4561 cgcgaggata aaccgcctgt tcaccatata gaaagaaaga ccgatacgcc agctgatgac
     4621 atgccggctg ataagcttta taaatacgcc tggcttgcgt tgtgtgtcct gccttcatca
     4681 agactggtgc gagaacagca cccccaaggt aaacgaccaa aagaattgcg acaagcacca
     4741 gcaaccacct ttgaaggcgg tcgcggtctt ttttgcttct tcgttttgcc aggcgttttc
     4801 ttgcaaattt ggccatctat tcaatgacct caactgtggt attgccaaca aagccctgaa
     4861 tagtctgctc tcccggatag tagctcacat aaggctcaga ccagcgccaa atccacttgg
     4921 catcatgggg caagcagttc acacagccat gcgaaagtgc cacgccaaaa ttgttgtgcc
     4981 agtaagcggt atgaatcgcc tgcccatcgc tgtggtacaa aagcgtccag gcaatacccg
     5041 gtgaatcgta ttgcgacacc ccacccgcgc tcatatgtaa cgaaatcatc ttgcgccagg
     5101 tatgatgcac gccgggcgtc gtcgtccatt ttccttcttt cacatagccg gtagacactt
     5161 cgcaaaaata aacttcccgt ttgccttcaa aggcgtacaa agtttgattg taaaggtcaa
     5221 ccacgatgcg cttttcgcca gcttcagggt gaatgggggc aatttcttcg ggcgtgatta
     5281 cccggcaagc ccgggcgtca acccaatatt gatctggcaa cgcgccatat ttctcactca
     5341 gcaaatactc cgtgcgccca ttgtgctgac ggatatccga cacccaatgc acttgcgaat
     5401 aataaagcct gggctggggc accgaacgaa tccaatagga tgaattttcc tttgaaccct
     5461 gcacctggat atccaccttg ggaatggtaa tctccaccca catcccgtgc tgaccatcca
     5521 cctgctcggg caaggcatta accaccggat tgtaataact gcgacaaggc tgcatcatcc
     5581 aggaatgcac ataaccttct ggcgtttcgt accagcgttg tttatagcgg ttggggtcca
     5641 gcttgttggc aaccagttca cgtttaatct caaaaacgtc atcgcggtaa atcgttttca
     5701 ccaccggcgc gtcaacattc ggctcgctgc gcatttcggt ccatgcgcca acatcgccaa
     5761 ccgcgatacg cccataaagc ttgccatcaa taaattgctc cacggcctgg gttcgcggtt
     5821 ccagcttagg caaggccaaa gcgcccatca ccatgccact cattttcaaa aattctcttc
     5881 ggctaatttt ccagtttttc atcatcacta ttatacggtc tcaactaaac attacaaaaa
     5941 agcttgtgca acgaaacact cccaggcttg ttttcagcca gggcgctaaa aatcaccgcc
     6001 ccggcgggag ctttcgcgct gaatatcgcg ttctttaatg ctttcgcgtt tgtcatattg
     6061 tttctttccg cgcgccacgc cgatttccag tttggcgcgt ccagctttca ggtagacccg
     6121 caaagggaca atcgtcatgc ccttaatgcg cacggcgttc cacaactggc ggatttcctt
     6181 cttgtgcaaa agcagtttgc ggtcgcgcgt cggattatgc tgcgcggtcg aagcctgctc
     6241 ataaggcgcg atatgcgcgc caaccagcca ggcctcctcg ccatcggtgc gcacataagc
     6301 ctcctgcagg ctcatctttc cctgacgaat cgacttaatc tcggtcccct tcagcaccag
     6361 tccggcttcg taagtatcgc tcacaaaata ttcaaaccgc gctttgcggt ttgtcgaaac
     6421 aactttaacg ttcatgagcc tattttaaca tattcccaaa agaatagcgc tgctaatgga
     6481 catgtgatat tagctcccaa aaatcctttg taatattcgt ataagaagca ctacgaaaaa
     6541 tacgatt
//
LOCUS       DAOSFC010000045         6530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10324_length_6530_cov_4.113977, whole genome shotgun sequence.
ACCESSION   DAOSFC010000045 DAOSFC010000000
VERSION     DAOSFC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10324_length_6530_cov_4.113977"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PK791_02065"
     CDS             complement(<1..195)
                     /locus_tag="PK791_02065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH92129.1"
                     /translation="MNLSIVVPVYNEEKNLPELYERIEAAMLPLGYPWNVTFVDDGSR
                     DKSLEVLKSLQSANPDKVRVV"
     gene            279..1319
                     /gene="ruvB"
                     /locus_tag="PK791_02070"
     CDS             279..1319
                     /gene="ruvB"
                     /locus_tag="PK791_02070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOH92130.1"
                     /translation="MTNEEKDNILNGRANSDDPIDNALRPTSLEDLIGQDVVKDNLRI
                     QIQAAKQRQEALDHVLFYGPPGLGKTTLSHILAAEMGVNIKITSGPAIERAGDLAAIL
                     TNLRANDILFIDEVHRLGRQVEEILYPAMEDYALDIIIGKGPAAKSVRLKLPRFTVVG
                     ATTRLALLTAPLRARFGALYRLDYYDVKALCDIIRRGARLLQVPAEENGIVEIARRAR
                     GTPRIALRMLRRVRDYAEVKSDGRITSDVAEAALNLQNIDRMGLDEMDRRILLTIIDK
                     YNGGPVGLNTIAAPVSEEADTIMDVVEPYLLQLGFIDRTSQGRVATQLAYEHLGRPYV
                     TSSSQMDLFSEE"
     gene            1339..2361
                     /gene="queA"
                     /locus_tag="PK791_02075"
     CDS             1339..2361
                     /gene="queA"
                     /locus_tag="PK791_02075"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOH92131.1"
                     /translation="MLKTNDFDYYLPENQIAQTPVEPRDHSRLFVYERASGKIEHKHF
                     YDIVDYLHTGDLLVVNQTRVIPARLYGQKLPGLGHIEVLLLQKQGELEWSCLIGGSGM
                     KIGRRLRIENGPEAEVIAVLEGAKRIVRFDEAIELFFDKAGHVPLPPYIHGYEGDPER
                     YQTVFNKTPGSAAAPTAGLHFTPELIQQLKDKGVSMATVDLQVGLDTFLPVTEDNPYE
                     HHIHTEWCSVPQETVEMIAQTKANGGRVIAVGTTAVRTLETLAQVSENGALRSWQGDT
                     SLFILPGYQFKVIDGMITNFHLPKSTLLMLVSAFVGRETILALYKTAIELNYRFFSFG
                     DAMLLL"
     gene            2458..4914
                     /locus_tag="PK791_02080"
     CDS             2458..4914
                     /locus_tag="PK791_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016636114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH92132.1"
                     /translation="MMRFDKFSERAQDAAARAAEIITRYGHNQVDSEHILLALIEQPD
                     GIVSQIFEMLKVDTIAVVDRLDEILRTTPKANIFGGASGQIFLTPRVKMVVDLAKTEA
                     TRLGDEYISTEHLLLAILAERNTQLARLLESHNITHQGVTDAIHELRGGKKGARPGSG
                     QYKTLEKYSRDLTQAAKDGKLDPIYGRDSEILRLITILSRRTKNNPVLIGEAGVGKTA
                     IAEGLAQKIANDDVPEILSGKRVVALDLGAMVAGTRFRGEFEERLKNTLEEAQNSDGE
                     IILFIDELHTVVGAGAAQGAMDASNMLKPALARGDLQCVGATTLDEYRKYIEKDAALE
                     RRFAPIYVEEPSIDDTVNMLKSLRDRYEAHHNVTLSDEALNAAAKLSSRYMTERKLPD
                     KAIDLMDEAAAKLRVALFSLPEELREEKKEIDRLAAEEEQAGLERDYERAVKLKAERL
                     RLEESFNESRAHWESEMKLDDVVDAEDIADVIHQWTGIPLTQLMESESQKLLQMESRL
                     SEKIIGQEEAIAAISDAIRRARSGLKNPDRPIGSFIFIGPSGVGKTELAKVLAEFLFD
                     DEEALVRIDMSEYREQHTVSRLFGAPPGYVGFEEGGQLTEAVRRRPYRVVLFDEIEKA
                     HPEVWNALLQILDDGRLTDGQGRVVDFRNTVLIMTSNLGTEFVRKGGTLGFIDKSGDD
                     DEKAAHDKIQNALKEAFRPEFLNRIDEIIIFSPLSKENLRSIVGLQVKELGERMAEFG
                     LTLEMDESAVNWLADESFDASFGARPIVRALQKFVESPLASKLLAGEFKEGDKIIARH
                     KEGDDTLTFSTTPSDNAEDE"
     gene            4946..5662
                     /locus_tag="PK791_02085"
     CDS             4946..5662
                     /locus_tag="PK791_02085"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH92133.1"
                     /translation="MFIARVLIIKEKYLLDPKFMKRMKNLFKNKDFLLILIYLLSLPI
                     FGLLNRPIGIVRNLYSPVDDVIPYVPQFILIYHSWFPFLMANAFALYKTQRIEYRKYM
                     MHLIVGQFAAYLTFIVFQTDVPRAIHLGDSIFDKLVALTYHVDNHYAGFPSVHALTTM
                     VLIMTVLRSKFKPGYKVFSVFYSVMILASILLVKQHVFWDLPSGIVYGVITFPLAMVI
                     LKHVFPEYLTYDNGQVLTKY"
     gene            5707..6228
                     /gene="def"
                     /locus_tag="PK791_02090"
     CDS             5707..6228
                     /gene="def"
                     /locus_tag="PK791_02090"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOH92134.1"
                     /translation="MDKYEIITHPNPFLRRKAKKVSVFDKDFQDLIEDMFVIMREAPG
                     VGLAAPQIGVSQRVVVVEYADAEVENAKPKAYVLVNPEIVYHSDDTTSDLEGCLSVPG
                     FVGMVERWNEVTVKAQNRFGKNIKIKADGWLARIFQHEMDHLDGVLYIDKATEVYEPS
                     DKEIDDIEPEPEN"
     gene            6230..>6530
                     /locus_tag="PK791_02095"
     CDS             6230..>6530
                     /locus_tag="PK791_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH92135.1"
                     /translation="MHLKRLSLTHFRSFTRLDLEFPRRVILFTGKNAQGKTSILEAIA
                     FLANFSSFHASHDRQLLNFNMPVEPIMVGRIIGEFERQEQSHTIEVRLIQEYTGQS"
BASE COUNT         1852 a         1260 c         1613 g         1805 t
ORIGIN      
        1 gaccacacga actttatcgg ggtttgctga ttgcaagctt ttgagaactt ccaggctttt
       61 gtcacggctg ccatcgtcca caaaagtgac gttccaggga tatcccaggg gaagcatagc
      121 ggcttcaata cgttcataaa gttcgggcag gtttttttct tcattatata cgggcacgac
      181 gattgataaa ttcataatgt ctccaaaatt tttgctgttg cgcctattat acctatcaaa
      241 tgcaggaatg ggacaatatt tcgggtataa ttttgctaat gactaacgaa gaaaaagata
      301 acattctcaa cggacgggca aacagtgatg atcccatcga caacgctttg cggccgacgt
      361 ccctggaaga tttaattggg caggatgtcg ttaaggataa tctgcgcatt cagattcagg
      421 ctgccaaaca acgccaggaa gccttagacc atgtattgtt ttacggtccg cctgggctgg
      481 gtaaaacgac actttcacac attctggcag ctgagatggg cgttaatatc aaaatcacgt
      541 ctggacctgc tatagaacgc gcaggcgatt tagctgctat tttgactaat ttgcgcgcta
      601 atgacatctt atttattgac gaagtgcatc gtttgggacg gcaagttgaa gaaatcttat
      661 atccggcgat ggaagactac gctttggata ttatcatcgg aaaaggacca gcagcaaaat
      721 ctgtgcgatt aaaactcccc cgatttactg ttgtgggggc gacgacacgt ttagccttat
      781 taaccgcgcc gttacgggct cgttttggcg cattgtatcg cctggattat tacgatgtca
      841 aagccctgtg cgatatcatt cgacgggggg caaggctttt gcaagtgcct gccgaagaaa
      901 atggcattgt tgaaatcgcc agacgggcgc gcggaacgcc acgcattgct ttgcggatgt
      961 taagacgtgt gcgtgattat gctgaagtga aatcagatgg acgcattacc agtgacgtgg
     1021 ctgaagccgc actcaacttg cagaatattg accgaatggg tttggacgaa atggacaggc
     1081 gtattcttct gactatcatc gacaaataca atgggggccc tgttggctta aatacaattg
     1141 ccgcaccggt tagcgaagaa gctgacacaa ttatggatgt ggtggagccc tatctcttgc
     1201 agctgggctt tatcgaccgt acttcgcagg gtcgggttgc tactcaactg gcgtatgaac
     1261 atctgggaag accctacgtg acaagcagca gtcaaatgga tttgttcagc gaagaataag
     1321 cctgccaaat taattacgat gcttaaaacc aacgattttg attattacct gccagaaaat
     1381 cagatcgcgc aaacgcctgt ggaaccgcgc gaccactccc gtttgtttgt ttatgaaaga
     1441 gcatctggaa aaattgaaca taagcatttt tatgatattg ttgactatct gcatacgggg
     1501 gatttgttag tcgtcaacca aacacgggtc attcctgcgc gtttatatgg ccaaaaactg
     1561 ccaggcttag gacacattga agttttgcta ttgcaaaagc aaggcgaact tgaatggtcg
     1621 tgtttaatcg gtggcagtgg catgaagata ggccggcgat tgcgtattga aaacggaccg
     1681 gaagctgaag ttattgccgt tttggagggc gctaaacgga ttgtccgctt tgatgaagct
     1741 atagaacttt tttttgataa agctggacat gtcccgttgc cgccgtatat tcatggttat
     1801 gaaggcgatc cggagcgtta tcaaaccgtg tttaataaga cgccaggttc tgctgctgcg
     1861 cctacggcgg gtttgcattt tacgccagag cttatccaac agctgaaaga taaaggcgtc
     1921 agtatggcaa ctgttgattt gcaagttggc ttggatacct ttttaccagt tactgaagac
     1981 aatccgtatg aacatcatat tcacaccgaa tggtgttctg tgccacagga aactgttgaa
     2041 atgattgcac aaacgaaagc taacggtggg cgtgtgattg cggttggtac taccgcagtt
     2101 cggactttgg agactctggc gcaagtaagt gaaaatggcg ctttaagatc ctggcaaggg
     2161 gatacatcac tttttatatt acctggatat caattcaaag ttatagatgg catgattacg
     2221 aattttcatt tgccgaaatc cacactttta atgctggtaa gcgcgttcgt tggccgagaa
     2281 acgattttag cattgtataa aacagctata gagttgaatt atcgtttctt ttcatttggc
     2341 gatgcgatgc ttttactgta aacaacagga atcttaaggt taatctaata attcttggct
     2401 acactgatac tgataaataa aggtacaaga taaaagaaat aaaagaggaa caaaactatg
     2461 atgcgttttg ataaattttc agaaagagcc caggatgccg ctgcccgcgc cgcggaaatc
     2521 attacacgct atgggcacaa tcaagtcgat tctgagcata ttttgctggc tctcattgag
     2581 caacccgatg ggattgttag ccaaatcttt gagatgctta aggttgatac catcgctgtg
     2641 gttgaccggc ttgatgaaat attgcgcacg acacccaagg cgaatatctt tggcggggcg
     2701 agtgggcaaa ttttcctgac accacgtgtg aaaatggtcg tggacctggc aaaaactgaa
     2761 gcaacgcgat taggtgatga atacatttca accgaacact tgcttctggc tatacttgct
     2821 gagagaaata cccaactggc gcggttgttg gaatcccata acattaccca tcaaggtgtg
     2881 acggatgcta tccatgaact gcgtggtggt aaaaaagggg cccggcctgg aagcggacaa
     2941 tataaaacgc ttgaaaaata ttcgcgcgac cttacccagg cagccaaaga tggcaagctt
     3001 gatccgattt atggccgtga ttcagaaata ttgcgtttga taaccatctt gagcagacgt
     3061 actaaaaaca atccagtact tatcggtgaa gctggtgtcg gaaagacagc gattgctgaa
     3121 ggactggccc agaaaatcgc gaacgatgac gtacctgaaa ttctgagtgg caaaagggtt
     3181 gtcgctttgg acttaggcgc gatggttgcg ggtacccgtt ttagaggtga gtttgaagag
     3241 cgcttgaaaa acacgctcga agaagcgcaa aactcggatg gtgagattat tcttttcatt
     3301 gatgaattgc atactgtggt tggggcgggt gcagcacaag gcgcgatgga cgcttcgaat
     3361 atgctgaaac ccgctttggc aagaggcgat ttgcaatgtg ttggcgcgac gactttggat
     3421 gaatatcgga agtacattga gaaagatgcc gcgctggaac gccgtttcgc acctatctat
     3481 gtagaagaac ccagcattga tgacaccgtc aatatgctaa aaagtctccg tgaccgttat
     3541 gaagcccatc ataatgtcac cctttctgat gaagctttga atgctgctgc gaaattaagt
     3601 tcgcgttata tgacggaacg gaaattgcct gataaagcga ttgacttgat ggacgaagcc
     3661 gcagcgaaat tgcgtgtggc gctattttct ttgcctgaag aattacgtga agaaaagaaa
     3721 gaaattgacc gtttggcagc tgaagaagaa caagctggct tggaacgtga ttatgaacgc
     3781 gctgtaaaac taaaagctga acgcttgcga cttgaggaaa gttttaatga aagtcgcgcg
     3841 cattgggaaa gcgagatgaa actggatgat gtcgtggatg cggaagatat cgcagatgtc
     3901 attcatcaat ggacaggtat ccctttgacg caactcatgg aatcggaatc tcaaaagttg
     3961 cttcaaatgg aaagccgctt atctgaaaaa attattggac aggaagaagc catcgcagct
     4021 atttctgatg caattcgacg cgcccgctca ggtcttaaga atcccgaccg accgattggc
     4081 tcgtttatct ttatcggacc ttctggtgtg ggtaagacgg aactagcaaa agtattagct
     4141 gaattcctgt ttgatgatga agaagcatta gtgcgcatag acatgagcga atatcgggag
     4201 caacacacag tttcgcgact ttttggcgcg ccacctggat atgttggatt tgaagaaggt
     4261 ggtcagctta ctgaagctgt ccgaagacga ccatatcggg tagtcctgtt tgatgaaatt
     4321 gaaaaagccc accctgaagt ttggaacgcg ctattgcaga ttctggacga tggacgattg
     4381 actgatggac agggcagagt ggttgacttt agaaatacgg ttttaatcat gaccagtaac
     4441 cttggtactg aatttgtgcg caagggcggt acgcttggtt tcatcgataa gagtggagac
     4501 gatgacgaaa aagctgcgca cgataagatc caaaacgcgt tgaaagaagc ctttagaccc
     4561 gaatttctca accggattga cgaaattatt atcttctcgc cattaagcaa ggaaaacctg
     4621 cgcagtattg ttggtttgca ggtgaaagaa cttggcgagc gcatggctga atttggcttg
     4681 actttagaaa tggatgaaag cgcggttaat tggctggctg atgagagttt tgacgcttcg
     4741 tttggtgcca gaccgattgt gcgcgctttg caaaagtttg ttgaaagtcc actggccagt
     4801 aagttgttgg ctggagaatt taaggaaggt gataaaatca ttgcccgaca taaagaaggt
     4861 gatgatacat tgacctttag tacgacacca agcgataatg ctgaagacga atagcataaa
     4921 acagaaccag aacaacacac gggtaatgtt tatcgcccgt gtgttgatta ttaaagagaa
     4981 gtatttacta gaccctaagt ttatgaaaag aatgaagaat ctgttcaaaa acaaagactt
     5041 tttacttatc ctgatttatt tactaagctt gccgattttt ggactgttga atcgtcccat
     5101 cggcattgtg cggaatcttt attcgcctgt tgatgatgtt attccgtatg tgccacaatt
     5161 tatcttgatt tatcacagtt ggtttccttt tttgatggcg aatgcttttg ctttatataa
     5221 aacgcaaaga attgaatatc gtaaatatat gatgcatttg atagttggtc agtttgctgc
     5281 ttatttgact tttatcgttt ttcaaacgga tgttccccgg gcgattcatt taggcgattc
     5341 tatctttgat aaactggtcg cattaaccta tcatgtggat aaccattatg ccggctttcc
     5401 atcggtgcat gctttaacaa cgatggtttt gattatgacg gtattacgtt caaagtttaa
     5461 gccaggatat aaagttttct cagttttcta ttcagtgatg attcttgcgt ctattctatt
     5521 ggtgaaacag catgtgtttt gggatttgcc cagtggcatt gtatatggag tgattacgtt
     5581 tccactggca atggttattc taaaacatgt ttttccagaa tatctgacgt atgacaatgg
     5641 acaagtgcta acaaaatact agaatttggc ctgtgcatga tggtataact aagctggaga
     5701 ttaattatgg acaagtatga aattattact cacccaaatc cttttttacg caggaaggcg
     5761 aaaaaggtga gtgtatttga taaggatttt caagacttga ttgaagatat gtttgtgatt
     5821 atgcgcgaag cacccggcgt gggacttgcc gcgccacaaa ttggtgttag ccagcgcgtt
     5881 gtcgtggttg aatatgcgga tgctgaagtt gaaaacgcca agccaaaggc atatgttttg
     5941 gttaatccag agattgttta ccattctgat gatacgacat cggatttgga aggatgtttg
     6001 agtgtgcctg gttttgtggg catggttgaa cgctggaacg aagtaactgt aaaagcgcaa
     6061 aaccgtttcg gaaaaaatat aaaaatcaag gctgatggct ggctggcacg tatttttcaa
     6121 catgaaatgg accatttgga tggggttctt tacattgata aagcaactga agtatatgaa
     6181 ccatcagata aagaaataga tgatatagaa ccagaaccag aaaactaaca tgcatttaaa
     6241 acgcctttcc ctgacacatt tccgttcttt tacccgtctg gatttagaat ttcccaggcg
     6301 ggtcatcttg tttaccggaa aaaacgctca aggaaagacg agtattttgg aagccatcgc
     6361 ttttttagca aacttttcat cttttcatgc cagccacgac agacaattgc tcaattttaa
     6421 tatgccagtt gaaccgatca tggtaggacg gattatcgga gagtttgaaa gacaggaaca
     6481 aagccatacg attgaagtgc gccttatcca ggaatatacc ggacaaagtc
//
LOCUS       DAOSFC010000046         6485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10421_length_6485_cov_4.585848, whole genome shotgun sequence.
ACCESSION   DAOSFC010000046 DAOSFC010000000
VERSION     DAOSFC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10421_length_6485_cov_4.585848"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PK791_02100"
     CDS             complement(<1..407)
                     /locus_tag="PK791_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92136.1"
                     /translation="MNIRIGKKACNYLFLLLITLLLVACGVVIQRHDQVHVIRAGTIP
                     SKVKLPNQVNVTFSVESPPVDLDNIQLALDILDDLSSYSDNIRRYPMQNKGDNHYEVN
                     ILLDEGFDIRYRYTMIEPAEVPETNVFGQLVDMR"
     gene            complement(413..841)
                     /gene="nusB"
                     /locus_tag="PK791_02105"
     CDS             complement(413..841)
                     /gene="nusB"
                     /locus_tag="PK791_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOH92137.1"
                     /translation="MKSRTKARFVALQSLYELDLTDHSLGDVLTQRSEDFSLSPNEYD
                     FALKLATGVMENSVKLDMLLSEHAPEWPLEQVAVIDRNILRIALWELAFYRKTPLKVG
                     INEAVELAKLFGTDSSPRFINGVLGSLAENMNKIIVSVGN"
     gene            complement(843..1181)
                     /locus_tag="PK791_02110"
     CDS             complement(843..1181)
                     /locus_tag="PK791_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOH92138.1"
                     /translation="MEHETVFNTTIDPSVLYTIVRLTTLATPGVDRMSSISFGANKVA
                     QENQGLKIEINDSKLFIDLYIVTELEENVRLVSETVQTNVSRAIYDMVGMEVANINVH
                     ITDVELMKAV"
     gene            complement(1202..1702)
                     /locus_tag="PK791_02115"
     CDS             complement(1202..1702)
                     /locus_tag="PK791_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92139.1"
                     /translation="MNQFYKPMVGLNVYLEPYFRSEIIEKVYANIPELSDEIRRDLIR
                     LTKDELKVPGFRNPMLAPLALRIRAAEKKFEKDSNFVKQILVSWSDLHRYLLNDSLES
                     LKTLGFNILDTATEYPDPENAFYIGWPEGINYKTIHEELSKNPDNKLTSDENSLLCIW
                     LTGYLP"
     gene            complement(1771..2025)
                     /locus_tag="PK791_02120"
     CDS             complement(1771..2025)
                     /locus_tag="PK791_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOH92140.1"
                     /translation="MAPRVRCHYNDCEFLDERYCSAAAIEINPDNGCLTYRPTEDIST
                     VAGWDDDESLDDWEEITDEDDDDLWVSLDDEEDEDLDEDY"
     gene            complement(2107..4302)
                     /locus_tag="PK791_02125"
     CDS             complement(2107..4302)
                     /locus_tag="PK791_02125"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOH92141.1"
                     /translation="MLLDQLLAKAPTTYTPADIASITEAYYFAEKAHAGQTRANGQPY
                     LTHCVGTATILMEMEFPPNMVVAGLLHDVLIDTDTKADVLYDKFGESVCRFVEGVTKI
                     TALPQLARADQHNGDSLLFEAQPTVSKSKFTENLSEFLRKMLLAIGDDIRVIIIKLAD
                     RLHNMRTLNALPPAHQKKIAQDTLDVYAPLANRLGIWQMKWELEDLGFRYTDPEKYRE
                     IAEHITAKGDARQAEIDEIINSFECLLSKADIKSKISGRPKHIYSIYMKMRNKDKSFD
                     AIRDLRAIRIIVDNIETCYRVLGLVHMNYQPIPGEFDDYIAAKKDNNYQSLHTAVIYK
                     DGKPLEIQIRTWEMHKNAEFGVAAHWRYKEKDAQVSSNYEAKLNAMRNLLAWSQEFEE
                     NHELDEQFRTDSFHDRIYALTPLGDVIELSAGSTPIDFAYQVHTDVGHRCRGAKVNNK
                     LVPLNYTLKTGDQVEILTANRGGPSRDWLNPSLGLVNSTRSRAKIRQWFKQQDRDQNL
                     AHGKLLIDKEFKRLGVDDIDVSELATQFNFKNIDDFYVAVGCDDIPITRVVGRIGELR
                     QAKLGEQELLVEQAKHDETDDVVQIMGLSGMLTQMARCCNPMPGDSIIGYITRGRGAT
                     IHRSNCPNVLRVKDPERLINVEWGTVQKTYPVPIQVNAYDRQGLISDISGVLSGETAR
                     LINFSMTSRHHIVKVNMVLEVPDITALSRILARLENLPNVIEAIRVSPG"
     gene            complement(4332..5492)
                     /locus_tag="PK791_02130"
     CDS             complement(4332..5492)
                     /locus_tag="PK791_02130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOH92142.1"
                     /translation="MTNTQNFDVIIAGAGSVGVPSAYYLADAGFSVLVIDPLASNGQG
                     SNKHAIGGVRATHTNLAKIKISRKSIEILSNWESEHGEDIEWHSGGYSFVAYDEPTKQ
                     DLLDIVHFQQSLNLNIQWLERDDMIALVPDINPDGLIGGTIAPEDGHASPLKVNYSYH
                     RLAVQAGAKFHFKEKVTGIIQNGGRVEGVITDKAKYSCSYFIDAAGSWSAEISKLIGI
                     DLKVKPDAHQGGITEAVAPMFDPMIIDLRPRPGSSNFYFYQHPSGKIVFCVTPEPSIY
                     GHFDDDTSDFLPYAAQRLIETMPRLANIRVRRTWRGTYPMTPDGNPIFGEYPGLKGFI
                     HATGTCGQGFMLGPGMGWYIKKLITNTLSDDERASMESLRLDRDYSEAEKLM"
     gene            complement(5485..>6485)
                     /locus_tag="PK791_02135"
     CDS             complement(5485..>6485)
                     /locus_tag="PK791_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOH92143.1"
                     /translation="SFIIKLSGYINTSNFSGDAKAIAEASAAIFSGKIAGVNIARELG
                     YETDADTQAWQSLEDVLSSRPGNIEKTKVDFPHEGMYPVMHCTQEIPCNPCSTVCPKQ
                     LIKVDKSDLRHLPYYASSSTDQCINCGRCVAVCPGLAVSIVDFRKQNGKALVSLPFEQ
                     DPEMIAVGNMIEVTNTDGSSLGNYPITEIRKIAGFRDGTRVVTIETPAEIATEAAGFR
                     IIKRDEPKPFVSEMELVENLADDAFICRCERVTAGEIRKLIREGVRDINQIKAKTKAS
                     MGACGGKTCLNMIKRLFQSEGISLDEVIETPIRPVFVEVPLHILGNIKADKEETHD"
BASE COUNT         1823 a         1371 c         1279 g         2012 t
ORIGIN      
        1 cgcatatcaa cgagttgtcc aaaaacattc gtttcgggta cttcagctgg ttcaatcatg
       61 gtatagcgat agcggatgtc aaacccttca tctagcagga tattaacttc ataatggtta
      121 tcgcccttgt tttgcatcgg ataacgtctg atattatcgg aatatgaact aaggtcgtca
      181 agtatatcca atgcaagttg tatattgtcc aaatcaacag gtggtgattc aacagaaaat
      241 gtcacattaa cctgatttgg tagcttgact tttgatggta ttgtgccagc tctaataaca
      301 tgaacctgat catggcgttg gattaccacg ccacaggcta caagcaatag tgtgattaat
      361 aacaagaata aataattgca agctttttta ccaatacgta tattcatatc gtttagtttc
      421 caacagaaac aattatttta ttcatatttt ctgcaaggct tcctaaaact ccgttgataa
      481 atcttggcga actatcagta ccgaataatt tggcaagttc aactgcttca ttaatgccta
      541 ctttaagcgg tgttttacga taaaaagcca gttcccacag cgcgattcgc aaaatgttac
      601 ggtcaatgac cgctacttgc tcaagaggcc attctggagc gtgttccgac aatagcatat
      661 cgagctttac actattttcc attacgcccg ttgctaattt tagtgcgaaa tcatattcat
      721 taggtgataa ggagaagtcc tcagatcttt gcgtaaggac atctcctaat gagtgatcgg
      781 tgagatctaa ttcgtatagg gattgcaagg ctacaaaacg cgccttggtt cttgatttca
      841 tattaaacag ctttcattag ttcaacatca gtaatatgca catttatatt agcaacttcc
      901 atgccaacca tatcataaat ggctcgcgat acattggttt gaacagtttc ggaaacaagt
      961 cgaacatttt cttctaattc agtaacaatg taaaggtcaa taaagagctt tgaatcatta
     1021 atttcaatct ttagcccttg attttcctgg gcgactttgt tagcgccaaa cgagattgat
     1081 gacattctgt caacgcctgg agttgccaat gtcgtcagcc gaacaatggt gtaaagcacg
     1141 cttggatcaa tagtagtgtt aaagacagtt tcgtgttcca taatctcctt gtttatcaca
     1201 attaaggtaa gtatccagta agccaaatgc acaaaagcga attttcatca cttgtcagtt
     1261 tgttatcagg atttttagaa agttcctcat gaattgtttt atagtttatt ccttcaggcc
     1321 agccaatata aaaagcattt tcaggatccg ggtactcagt tgcagtgtcc aggatgttga
     1381 aacctagtgt tttcaaagat tctaagctgt cattaagtaa atatcgatgc aaatctgacc
     1441 aggaaactaa aatttgtttt acaaaattag aatccttttc aaactttttc tcagcagccc
     1501 taatgcgtaa tgccaatggt gccaacattg gatttctgaa accagggact tttaattcat
     1561 ctttagtgag cctgattaaa tcacggcgaa tctcatcgga aagttctggt atattcgcat
     1621 aaaccttttc tattatttct gaacgaaaat aaggttcaag atagacatta agtccaacca
     1681 tcggtttata aaattggttc ataactttct ccagatttct aattttaaag tttgctatgt
     1741 tcaacccttc attataccgc gtagttagtt ttaatagtct tcgtccaaat cttcatcttc
     1801 ttcgtcgtct agactaaccc aaaggtcatc atcatcttca tcagttattt cttcccaatc
     1861 atctaagctt tcatcatcat cccatccggc aacagtagaa atatcttcag taggacgata
     1921 ggttaaacat ccgttatctg gatttatttc tattgctgca gcgctacaat agcgttcatc
     1981 cagaaactca caatcgttgt agtgacatct aactcgtggt gccatatagt atgttctcca
     2041 aagatatata tattttaaaa ccagtgcatt ttacactagc ttacataaac cgtaaagtca
     2101 tttgacttaa cccggtgaaa cgcgaatagc ttcgattacg tttggaagat tttcaagtct
     2161 ggctaaaata cggcttagcg cggtgatatc aggtacttcc aataccatat ttacttttac
     2221 aatgtggtga cgcgaagtca ttgaaaagtt gattaatctt gcagtttctc cagataaaac
     2281 acctgaaata tcagatatca atccctgtct gtcgtaagcg tttacttgta ttggaacagg
     2341 ataggtcttt tgaaccgttc cccattctac atttatgagt ctttcagggt cttttacacg
     2401 tagaacattt gggcaattgg accgatgaat tgtggcacca cgtcctcttg taatataccc
     2461 gatgatagaa tcaccaggca taggattaca gcagcgcgcc atttgagtga gcatacctga
     2521 aagtcccatg atttgaacga catcatccgt ttcgtcatgt ttggcttgct caactaaaag
     2581 ttcttgttcg ccaagtttcg cttgtcttaa ttcaccgatt ctgccaacaa cacgtgtgat
     2641 aggaatgtcg tcacaaccaa cagctacata aaaatcatcg atatttttaa aattgaattg
     2701 tgtagccagt tcgcttacat cgatatcatc aacaccaagg cgcttaaatt ctttatcaat
     2761 aagcaacttg ccatgggcta ggttttggtc acggtcttgt tgtttgaacc attgacgtat
     2821 ttttgcgcgt gatcgggttg aatttacgag accaagacta gggtttaacc agtctcgact
     2881 aggcccacca cgattcgcag ttaaaatctc aacctggtca cccgttttta aggtgtaatt
     2941 gaggggcaca agtttgttgt ttacttttgc tccccgacat ctatggccta catccgtatg
     3001 aacctgatag gcaaaatcaa ttggcgtaga acctgctgat aattcaatta catctcccag
     3061 gggggtaaga gcataaatcc ggtcatggaa tgaatcagtt cggaattgct catctagttc
     3121 atgattctct tcaaattcct ggctccacgc aagtaaattg cgcatagcat taagctttgc
     3181 ttcataattg ctcgaaacct gggcgtcttt ttctttataa cgccagtggg ctgcaacgcc
     3241 aaactctgcg tttttatgca tctcccatgt gcgtatttgt atttctaagg gtttgccatc
     3301 tttatatata accgcagtat gcagcgattg ataattatta tccttttttg cagcgatgta
     3361 atcatcaaat tcgccgggaa tgggttgata attcatgtgt acaaggccca aaactcgata
     3421 acaggtttca atattgtcga caataattct aattgcgcgt aaatcccgga tagcatcaaa
     3481 gcttttatcc ttattacgca ttttcatgta aattgaataa atatgtttgg gacgaccaga
     3541 aattttagat ttaatgtctg ctttacttag taaacattca aaagagttaa ttatctcatc
     3601 aatttccgct tgtcgcgcgt caccttttgc tgtgatatgt tcagcaattt cacggtattt
     3661 ttctgggtcg gtatatctaa agccaaggtc ttcaagctcc cacttcattt gccaaatacc
     3721 aagacgattg gcgagtgggg catatacgtc aagtgtatct tgggcaattt tcttttgatg
     3781 tgctggaggc agggcattaa gcgttcgcat attatgcaat cgatcagcca acttgatgat
     3841 aatcacacga atatcatcac ctattgccaa aagcattttg cgcaggaatt ctgaaaggtt
     3901 ttcggtaaat tttgattttg aaaccgtagg ttgcgcttca aaaagtaaag agtcaccatt
     3961 gtgctggtca gctcgggcaa gctggggcag ggcggtaatt ttggtaacac cctcaacgaa
     4021 acgacaaacg gattctccaa atttgtcgta taaaacatcc gctttagtgt cagtgtcgat
     4081 aagcacatca tgtagcaggc cagcaacgac catgttgggt ggaaattcca tttccatcaa
     4141 tatagtggct gtgccaacac aatgtgtcag gtagggttgc ccatttgcac gtgtttggcc
     4201 tgcatgtgct ttttcagcga agtaataggc ttccgtaatc gatgcaatgt cagcgggtgt
     4261 gtatgttgtt ggtgctttcg ctaaaagctg gtcaagtaac atttcatcaa taatatacta
     4321 caaattgcgt actacataag tttttctgct tcagaataat cacggtccag acgtagggat
     4381 tccatgcttg cacgttcatc atccgaaagc gtatttgtaa tgagtttctt tatataccat
     4441 cccattcccg gacccagcat aaaaccttgt ccgcaggtgc ctgtggcgtg gataaatcct
     4501 ttcaaaccag gatactcgcc aaagatagga tttccatcgg gtgtcatggg ataggttcca
     4561 cgccaggttc ttctaacgcg gatgttagct aaacgtggca tggtctcaat aagacgttgt
     4621 gcggcataag gtaaaaagtc actcgtgtca tcatcaaaat gaccgtagat ggagggttca
     4681 ggtgtaacgc aaaaaacaat cttcccggac ggatgctggt aaaaatagaa gttactcgaa
     4741 ccaggtctgg ggcgcaagtc gataatcatc ggatcaaaca tcggtgcgac tgcttcggta
     4801 atgccacctt gatgggcatc tggtttaact tttaaatcta tgccaatgag ttttgatatt
     4861 tcagctgacc atgaaccggc agcatcaata aagtaggaac agctatattt tgctttatcg
     4921 gttataacac cttcaacacg tccaccattt tgaataatgc cagtgacttt ttctttgaaa
     4981 tgaaatttcg cgccagcttg aacggccaag cgatgataag aataatttac tttcaaaggc
     5041 gatgcgtggc catcttcagg tgctatcgta ccgccaatca gaccatctgg gttgatgtcc
     5101 ggcaccaagg caatcatgtc atcacgttct aaccactgaa tgttgagatt tagtgattgc
     5161 tgaaaatgta caatatcaag caaatcttgt tttgttggtt catcataagc gacaaaacta
     5221 tagccaccag aatgccactc aatatcttct ccatgttcag attcccaatt tgaaagaatc
     5281 tcgatggatt tacgacttat ttttattttc gctaaatttg tatgggttgc ccttacgcca
     5341 ccaatagcgt gtttgtttga accctgacca ttgctggcaa gtggatcaat gaccaataca
     5401 ctaaatcctg catctgccaa ataataagca cttgggacac ctaccgaacc agctccagcg
     5461 atgatgacgt caaaattttg tgtattagtc atgtgtttct tccttatctg cttttatgtt
     5521 gcctaagatg tgaaggggaa cttcaacaaa aactgggcgt atcggggttt cgattacttc
     5581 atccaggctg ataccttcgc tttggaaaag gcgtttaatc atatttaggc atgttttacc
     5641 gccacaggct cccatgctgg ctttagtttt ggctttgatt tggttgatat cccgcacacc
     5701 ttcgcgtatc aatttgcgga tttctccagc agtaacacgt tcacatctac aaatgaaagc
     5761 gtcatcagcc aaattttcca ctaattccat ttcagacaca aaaggtttag gctcatcacg
     5821 cttaatgatt cgaaaacctg ctgcttcggt tgcaatctct gctggcgttt cgatagtcac
     5881 aacacgtgtg ccatccctaa agccggcaat tttacgaatt tcggttatgg ggtaatttcc
     5941 aaggctactt ccatccgtgt ttgtgacttc aatcatattc ccaacggcaa tcatttccgg
     6001 atcttgttcg aatggaagac ttaccaaagc tttaccattc tgctttcgaa agtcgacgat
     6061 actgacagcc aatccaggac aaacggcaac acagcgtccg caattaatgc actggtcggt
     6121 tgaagatgaa gcataatagg gcaggtggcg caggtcagat ttatcgacct ttataagttg
     6181 tttagggcac acagttgaac aagggttgca agggatttcc tgcgtgcagt gcatgactgg
     6241 atacatccct tcatgtggaa aatcaacttt agtcttttcg atatttcctg gtcgtgaact
     6301 caggacatct tcaagacttt gccaggcttg tgtatctgcg tcagtttcat aaccaagctc
     6361 tctggctata ttaacaccag caatttttcc ggagaatatc gcagcactgg cttcagcaat
     6421 cgctttggca tcaccggaaa aattgctggt gttaatatag ccagagagct tgattatgaa
     6481 actga
//
LOCUS       DAOSFC010000047         6450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10495_length_6450_cov_5.807975, whole genome shotgun sequence.
ACCESSION   DAOSFC010000047 DAOSFC010000000
VERSION     DAOSFC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10495_length_6450_cov_5.807975"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PK791_02140"
     CDS             <1..940
                     /locus_tag="PK791_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOH92144.1"
                     /translation="RLYDWQVSNEQVQYVPGIVTGFNVAVRGFCKPGEEVIYQVPAYP
                     PFIRAPRGAGMQGVQNAMVLGESGQYEVDFELFERQIIDNKVRVFILCNPHNPTGRVY
                     RRDELEKFAEICLRHDVIIVSDEIHCDILYDDRKHIPIAGLSKEISDVTITLQAPSKT
                     YNIAGLHTSVAIIQNEELRQRYNASKAGLVGTPCILGMYAAKAAYEHGDAWLQAQLAY
                     LQANRDLIDEALQTDLKPIKWAKPEATFLAWLDCRDLGVEGSMQEFFLDRAKVGFNDG
                     PEFGEVGQGFVRLNFGTTRANIQLAIEKMKAAIESL"
     gene            complement(1028..1243)
                     /locus_tag="PK791_02145"
     CDS             complement(1028..1243)
                     /locus_tag="PK791_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOH92145.1"
                     /translation="MSERSIGTIKWFSAPKGYGFIGREDGEDIFVHFSAIQMDGYRRL
                     NEGQKVEFSIEEGSKGKQAANVVPIND"
     gene            complement(1677..2600)
                     /locus_tag="PK791_02150"
     CDS             complement(1677..2600)
                     /locus_tag="PK791_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOH92146.1"
                     /translation="MAPMPEGRVFNAVIANGNYVYVIGGTSDAIGNTPTSTNFRYNTV
                     NNTWQTMSPMPVALDSIDGAVINDKIYIPGGGDDSNTYVYDIAADSWTTIPANGGYIA
                     RQQYQVVAIGTDLYVLGGLEGNASTNRVWKLDTITQTWSESIPMLKSRTSFSAAAING
                     TIYVAGGVSYPGFTPDMTAEKFDGTAWSFIAPLPDGDGAYTRWSYMADGSNANTMWLA
                     AGRRDAEWGALNHAAYYDPISDTWTDSPTIPILKQARVYMEGDIASDGYFYVIGGRAS
                     DASAVYDTNERLKVGTDAVTVDYLYLPLILN"
     gene            complement(2607..2789)
                     /locus_tag="PK791_02155"
     CDS             complement(2607..2789)
                     /locus_tag="PK791_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92147.1"
                     /translation="MKKTKFWIVFAVFVLLQVLTGAALAAPSAQKITSETGIASGTAV
                     IGEASGGIKPADIGGA"
     gene            2954..3793
                     /locus_tag="PK791_02160"
     CDS             2954..3793
                     /locus_tag="PK791_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92148.1"
                     /translation="MKMNSTSAVEESYNNSTYLREEQYQSADNLNARIEIHRRFSTAK
                     KEWNDFIFEHIPFTQANFKGLALGCGNATQWRENQSRFPRDTRMVLSEFSYGMLQEPR
                     FAFAEDERFQLCTMDAQHISFASNSFDFVSANHMLYHVPDIALALSEVWRVLKADGSF
                     MVATNGEHHMTELDQLLESFNPVYQGEHAMSSRFTLQNGENQLFEFFDEVALHPYQSD
                     LWVTDAGLLCDYAWSTPKVQLLFRPEQKESLRQFFEARIQADGGIFIGKDTGLFIARK
                     PRK"
     gene            complement(3768..4589)
                     /gene="uppP"
                     /locus_tag="PK791_02165"
     CDS             complement(3768..4589)
                     /gene="uppP"
                     /locus_tag="PK791_02165"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HOH92149.1"
                     /translation="MSYFQAIILGIVQGLTEFLPVSSSGHLVLVPFLLNWNLDPQKAF
                     LFDVLVQLGTLVAVFVYFRQDLLDILKAFFAGIKDRKPFQSAESRLAWYIILATIPAG
                     LIGLLLQDQVEALFDSPVWTAVFLFGTGVLLTVSELWSKKTRDLETMTVKDSLIIGFF
                     QALSIFPGVSRSGSTITGGLTQGFKRQAAARFSFLMSVPIMLAAGLLSVLKLIKMDGV
                     LEFLPVLLLGFVIAGVVGYFAIKWFIGYLKEKSLLPFAAYCAVVAIVVIFVAYAR"
     gene            complement(4586..5713)
                     /gene="tgt"
                     /locus_tag="PK791_02170"
     CDS             complement(4586..5713)
                     /gene="tgt"
                     /locus_tag="PK791_02170"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOH92150.1"
                     /translation="MKSTFSWKTLATDNRARAGEFSTPHGTLQTPLFAPVGTQATVKA
                     ITPAQLSEINPELVLANTYHLFLRPGEDIVAELGGLHVFMNWDGPILTDSGGFQVFSL
                     ASTRKIDDDGVTFRSHIDGSEKRLSPEISIDIQEKLGADIIMAFDECAKPLDFDYAKE
                     AMQRTHVWAERCLSAKKRPDQALFGIVQGGIFRDLREESARTISSMGFPGHAIGGLSV
                     GESKEDMYRTIEYVNAILPADKPRYLMGVGTQEDLIEGVLRGVDIFDCVHPTRLARHG
                     AAMTKTGRLNMKGVIYARDPLAIDPDCACYTCQNFSRAYIRHLLVAKEILASTLLSIH
                     NLHTLVQLTRDLRQAIIGGYLDDFAAEALAKLNLSRVPEVL"
     gene            5840..>6450
                     /locus_tag="PK791_02175"
     CDS             5840..>6450
                     /locus_tag="PK791_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOH92151.1"
                     /translation="MEESRNNYVRAEFHCHTVYSPDSLVKVRDLLDACAVKGVTKLAI
                     TDHGAMGGAFEAKALAPERIVLAEEIQTTEGELLAYFMTEPIPQYLPVMEVIERLKAQ
                     GAYISIAHPYDPYRGSHWEDETLEAIAPLVDGVEVFNARCIRQSFNDKAYAFAKAYDL
                     QALVGSDAHSLHELGKATLTMPDFNSPEELRESLKQAEFSGEL"
BASE COUNT         1709 a         1443 c         1630 g         1668 t
ORIGIN      
        1 acgattgtac gactggcaag taagtaatga gcaggtgcaa tatgtgccgg gtattgtgac
       61 cggattcaac gtggctgtgc gtggtttttg taagccgggt gaagaggtta tttatcaggt
      121 gcctgcttat ccgcccttta ttcgcgcgcc acgtggcgcg ggcatgcagg gtgtgcaaaa
      181 tgccatggtt ctcggtgaaa gtggtcagta cgaagttgac tttgagcttt ttgagcgtca
      241 aataatcgat aacaaggtgc gggtctttat tctgtgcaat ccacacaatc caactggccg
      301 tgtgtatcgc cgtgatgagc tggaaaagtt tgctgagatt tgcttgcgtc atgacgttat
      361 catcgtttcc gatgaaatcc attgtgatat cttgtatgac gatcggaagc atattcccat
      421 tgcgggcttg agcaaagaaa tctcggacgt gaccatcact ttgcaagcac ccagcaagac
      481 ttataatatt gctggtttgc acacttcggt ggcgatcatt cagaatgaag aactgcgtca
      541 gcgctataac gcgagcaagg ctgggcttgt tggtaccccg tgcattttag gaatgtacgc
      601 agccaaagcg gcttatgaac atggcgatgc ctggttgcag gcgcagttgg catacttgca
      661 ggccaaccgg gatttgattg atgaagcctt gcagaccgat ttgaagccaa ttaagtgggc
      721 caagcctgaa gcgactttcc tggcctggct tgattgtcgc gatttgggtg ttgaggggtc
      781 aatgcaggag ttcttccttg accgcgcgaa agttggtttt aacgatggcc ctgagtttgg
      841 cgaagttgga cagggctttg tgcgtctgaa ctttggaacc acgcgtgcca atatccagct
      901 ggccattgaa aagatgaaag ccgcgattga gagtctttag ttaaagaact taattaaact
      961 tgtgcttgaa cagaaaagat taaacaaaaa cgagccggga aaccggctcg ttttatgaac
     1021 ctaatgatta atcattaatg gggacaacgt tggcggcttg cttgcccttg ctgccttctt
     1081 caatgctgaa ttcgactttc tggccttcat tgaggcgtcg gtagccatcc atttggattg
     1141 ccgagaaatg aacgaaaata tcttcgccat cttcccggcc aataaagccg taaccttttg
     1201 gtgcgctgaa ccacttaatc gtaccaatac tgcgttctga cattctaaat aaactcaact
     1261 ttctttcaat gattgttttc ttgcttttgc aagtccgata aatttatcat tgacctgcgg
     1321 aaacgtcaag cacttaaaag gctgctgttt aaaaaacgtg tcgtgaaatt aatgtatgtg
     1381 ctgataagtt ttgcgctgta aaggaactac agtgcgcttt gattatcaaa atacatcaag
     1441 taaataatgt tttaactaaa caacaaaata ttgtggaggt atgaaaaatt atagctatct
     1501 tattaattat ttgcagtttc gtttggataa actctgaacc ctgtcagatt agctgtattt
     1561 gattgcaagc aagcgtaaga gagaatatat atcaggcaga aaattatgag aatcttccgt
     1621 ttaaaaaaga ggctaccaac agacagcctc tttttctcag atttagtcaa atagttttaa
     1681 ttcaggatta gtggcagata gagataatcg acggtgacag cgtcggtacc aacctttaaa
     1741 cgctcattgg tatcatacac ggccgaggca tcacttgcac gtccgccaat aacatagaag
     1801 tatccatcgc tggctatgtc accttccatg taaacgcgtg cttgcttcaa aatggggatg
     1861 gttggtgaat ctgtccaggt atccgatata ggatcataat aagcagcatg gtttagggcg
     1921 ccccactcgg catcgcgtct tcctgctgcc agccacatag tgtttgcatt actgccatca
     1981 gccatgtatg accaacgggt gtatgcaccg tcaccgtccg gcaatggtgc aataaaactc
     2041 cacgcggtac catcaaactt ttctgcagtc atgtcggggg taaacccagg gtacgaaaca
     2101 cctccagcca cgtaaatcgt gccattaatg gctgcagctg agaagctcgt gcgagatttt
     2161 agcataggaa tgctttctga ccaggtttgt gtgatggtat ctaatttcca gactcggttt
     2221 gtacttgcgt taccctcaag cccacctaac acatacaggt ctgtaccgat tgcgactacc
     2281 tgatactgtt gtctagctat ataaccacca ttggctggaa tagttgtcca ggaatcagca
     2341 gcaatatcat aaacataagt gttactgtca tctccgccac cgggaatgta gattttgtcg
     2401 ttaatgacag ccccatcaat ggaatcgagt gctacaggca ttggagacat ggtttgccag
     2461 gtgttattta cggtattgta acggaaatta gttgaggttg gtgtatttcc tatcgcatca
     2521 ctggtaccgc caattacata aacatagttt ccattggcga tgacggcatt aaacacacgt
     2581 ccttcgggca ttggtgccat ggtttcctag gcaccaccaa tatcggcagg cttaataccc
     2641 ccggaagctt cgccaatcac agctgttcca gacgcgatac cggtctcgct ggtgatcttt
     2701 tgggctgatg gtgctgccag agccgcgcct gttaagactt gcaagagtac aaatactgca
     2761 aaaacaatcc aaaacttagt tttcttcatt gtgacttttc ctccttagag attaaaaaaa
     2821 ttattgctat caaagaacta ttatagcact atcattgata acatgagaaa attaattatt
     2881 gagtttgctc ttgaacaggt atttggtgct tcataaagca gatttcatat tgcctgaaag
     2941 gtataataag ggcatgaaaa tgaattccac ttctgctgtt gaagaaagtt ataacaattc
     3001 cacatacctt cgcgaagagc aataccaaag tgcggataat ctaaacgcgc gaattgagat
     3061 tcacaggcgt tttagcactg ctaaaaagga atggaacgat ttcatttttg aacatatccc
     3121 ctttacgcaa gccaacttta aagggctggc actgggttgt ggcaacgcaa cgcaatggcg
     3181 tgaaaatcaa tcccgttttc cgcgagatac gcgcatggtt ttgagtgagt tttcctatgg
     3241 catgttgcag gagcccagat ttgccttcgc ggaagatgag cgattccaac tttgcacgat
     3301 ggatgcccag cacatcagtt ttgcgtcaaa tagttttgat tttgtcagtg caaatcacat
     3361 gctttatcac gtgccggata tcgcgctggc actgtctgaa gtctggcgtg tgctaaaagc
     3421 ggatggcagt tttatggtag ctaccaacgg tgagcatcac atgaccgaac tcgaccagct
     3481 tttggaatct tttaacccgg tatatcaggg cgagcacgct atgtccagcc ggtttacttt
     3541 gcaaaacgga gaaaatcagc tttttgagtt tttcgacgaa gtggcgttgc acccttacca
     3601 aagtgacttg tgggtgacgg acgctggctt gctatgcgac tatgcctggt cgacgccaaa
     3661 ggtgcagttg cttttcagac ctgaacaaaa agaaagcctg cgtcagttct tcgaggccag
     3721 gattcaagct gatggtggaa tctttatcgg gaaagatacc ggattgttta tcgcgcgtaa
     3781 gccacgaaaa taaccacaat tgctaccacc gcgcaataag ccgcgaaggg taaaagcgat
     3841 ttttccttta gatagccaat gaaccatttg atggcaaaat aacctacaac gccggcaatt
     3901 acaaagccca aaagcaacac aggcaaaaac tctaaaacgc catccatttt gatgagcttc
     3961 agcacactga gcaaacccgc ggctaacatg atagggacag acattaaaaa agaaaaacgg
     4021 gcagcggcct ggcgtttaaa gccctgtgtg agtccgccgg taatagtcga ccctgaacgc
     4081 gatacgccgg ggaatatgga taatgcctga aagaaaccga tgatgagcga atctttgacg
     4141 gtcatggttt caagatcgcg tgtcttttta gaccaaagtt cgctgaccgt gagcaaaacg
     4201 cctgttccga atagaaatac cgctgtccaa acggggctgt cgaacaaagc ttcgacctgg
     4261 tcctggagca acagtccaat caagccagcg ggaatcgttg ccaggatgat ataccaagcc
     4321 aggcgacttt cggcactctg gaatggtttg cggtctttta tgccggcaaa aaaggctttg
     4381 agaatgtcca acaaatcctg gcggaagtag acaaaaacag ccactaaagt gcctaactgc
     4441 accagcacgt caaacaggaa agctttttgt gggtcaaggt tccagttgag cagaaaggga
     4501 accagaacca aatgtccgga gcttgaaaca ggcagaaact cggtgagtcc ctgaacgata
     4561 cctaagatga tagcttgaaa atagctcata gcacctctgg aacacgcgaa aggtttagtt
     4621 ttgccagggc ttcggcggcg aagtcgtcca aatacccgcc gatgatagcc tggcgcaaat
     4681 cacgtgtcaa ttgcactaaa gtgtgcaggt tgtgaataga gagcaaggtt gatgccagaa
     4741 tttctttggc gaccagcaaa tggcggatat aagcgcggga gaagttttgg caggtgtaac
     4801 aggcgcagtc ggggtcaatg gccagtgggt cgcgcgcgta gatgacgcct ttcatgttga
     4861 gccggccggt cttagtcatg gcagcaccat ggcgtgccag gcgtgtgggg tgcacgcaat
     4921 caaaaatgtc aacaccacgc aaaacgcctt caatcaggtc ttcctgagta ccgacgccca
     4981 tcagatagcg gggtttatca gcgggtagaa tcgcgttgac gtactcgatg gtgcggtaca
     5041 tgtcttcttt gctttcgcca actgaaagac cgcctatggc gtggccaggg aaacccatgc
     5101 tggaaattgt tcgcgcggat tcttcgcgca aatccctaaa gatgccgcct tgcacgatgc
     5161 caaacagggc ctgatcggga cgttttttgg cgcttaggca acgttccgcc caaacgtggg
     5221 tgcgctgcat ggcttctttc gcgtagtcga agtccagggg tttggcacat tcatcaaagg
     5281 ccatgatgat gtccgcgccg agtttttcct gaatgtcaat cgagatttcc ggacttagac
     5341 gtttttcgga gccgtcgatg tgtgagcgaa aggtaacgcc atcatcgtca attttgcgcg
     5401 tacttgccag cgaaaagacc tgaaagccac ctgagtcggt caagatgggc ccatcccagt
     5461 tcataaagac gtgcaagccg cccagttccg cgacgatgtc ttcacccggg cgcaaaaaga
     5521 ggtgataggt gttggcgagc acgagttcgg gattaatctc gctcaattga gcgggtgtga
     5581 ttgccttgac ggtggcttgg gtgccgaccg gcgcgaaaag cggcgtttgc agggtgccat
     5641 ggggcgtgct aaactcgcca gcacgggcac ggttatctgt cgcgagagtt ttccagctaa
     5701 acgttgattt catttcagta tgtggcgcct tcgtttgtgt ctcggactta ttctatctta
     5761 atttggcagt aaacgggaaa tggtgaaagt tgactgagct ttgcgcatca actttagaac
     5821 aaggtataat ttgcgcgtta tggaagagtc tagaaacaat tatgtacgcg ctgaatttca
     5881 ttgccacacc gtctattcgc cggatagctt ggtgaaggta agagaccttc tcgacgcttg
     5941 tgctgtgaaa ggcgtcacga agttggcgat tacagaccat ggcgcgatgg gcggcgcgtt
     6001 tgaagcgaaa gctttggcgc ctgagcgaat cgtcctggca gaagaaattc aaaccacgga
     6061 aggcgaattg ctggcttatt ttatgactga gcccatccca cagtatttgc cggtgatgga
     6121 ggtcattgaa cgcttaaagg cgcaaggcgc gtatatcagc atcgcgcatc cttatgaccc
     6181 ctatcgtggc agccactggg aagatgaaac gcttgaggct atcgctcctt tggtggatgg
     6241 tgtggaagtg ttcaacgcgc gttgtatcag gcaaagcttt aatgacaaag cttatgcttt
     6301 tgcaaaagca tatgacttac aggctttggt aggatcagac gcgcacagtt tgcatgaact
     6361 cggcaaagcg accttgacca tgcccgactt taacagcccg gaagaactgc gggaatcttt
     6421 gaaacaagca gagttctccg gcgaactttt
//
LOCUS       DAOSFC010000048         6404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10614_length_6404_cov_4.184281, whole genome shotgun sequence.
ACCESSION   DAOSFC010000048 DAOSFC010000000
VERSION     DAOSFC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10614_length_6404_cov_4.184281"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /locus_tag="PK791_02180"
     CDS             <1..1802
                     /locus_tag="PK791_02180"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOH92152.1"
                     /translation="SWWQASRVLRDTSLSGHSIEVIDEFPQFTFLLADIHPHMLGMPF
                     VLIAIALALNFIRGGAEGRFKMAKLLVAYEPLAVFFAVLTLGGIAFMNTWDFPFYLAL
                     IAATLVYRRYLEDGWSGKRIIEFFGIVIICGFLSILIYLPFYLSFASQAGGILPSLAF
                     FTPGINFWVMFAPLLVPISFFLLYLVIKKKAFKELGLSVLIVLALFAFLFVFSWGIGW
                     LGSRSETTSATILGLQGAPDSRTLLTESIRLRLKSPGTAITLFALLSLGMASVLDRKK
                     IQNAETNSDVIIKEQTEKPVHLIFVLFLIILGALLTLAPEFVYLRDQFSWRMNTIFKF
                     YFQAWILWSLAASYAIVFLFGKSGTRRVPNSLFVMLIAGLVFTSFGLSLNERSTFPAG
                     FGALRADWLVLGIGLVFLLWIVSHLIRPNLKAVLAVVSVLVVLAGLIYPILEIWNKTE
                     GFQPRLGFTLDGKRVFWESYPDAMRAAEWLETAPMGVLAEAVADQGGSYTAYNLISTF
                     SGMPSVLGWVGHEHQWRGGGAEVGTRQQDLRELYSTKNMDRFKEIILQYNIRYIVYGN
                     YERDTYRVSEPYFSSELTPVFESDTVTIYEVRQ"
     gene            1804..3486
                     /locus_tag="PK791_02185"
     CDS             1804..3486
                     /locus_tag="PK791_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92153.1"
                     /translation="MQEKRQIPILKDRRELIWLLLIIFLAALVRLIKLGAMPFNAVDA
                     SLAADALAIARNQTLGQSALPAYTGLTGVLFYILGSSNFVARLLSVFAGVSLVVLSFL
                     LAKQKSLRVAIIFSLALALDPLMVNWSRQIATPMIALAGLAWAVCLFDKKRYIIAGFC
                     LAVAFLGGYSFWVFSLLALALWVFARVIKFEVPFFINTLPEKSAFLKTSLSFLISLVL
                     ISTGFFLEPTGFGQIGASLMAFLGLFKKAYQFPVYHSFFVLIRFALLPLLGAIIWLVS
                     RGNLSRKEKWMIAFYFLVSVLFGLFFSRTDVGLSILILIPLWYFTARYLSEFRLDINE
                     RLSLKIVCFVLFLVMFIYIAINLPRLIEAEFGSPQLIYAGLATLAGVLLVLIVFWLGS
                     LSLGMKQSSAILATALVLVLISSSLALSFNSLDKNRDTSQVMVNAEPILLANEAELTA
                     INPFQDYGKLNFVEASFLVEDIAYDLRWFTREFQAESSLRAPEFVITHTENPPNYDEP
                     YRGMPIELSRNMPWTQLNFAGYFNAIIAPERAIQVENANLWALTKLFTGAIQ"
     gene            3487..4194
                     /gene="cmk"
                     /locus_tag="PK791_02190"
     CDS             3487..4194
                     /gene="cmk"
                     /locus_tag="PK791_02190"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOH92154.1"
                     /translation="MKDKAEKTIPEIIAIDGPAASGKSTVGALVAEKLDYVWLDTGIM
                     YRAVACEALETGVPIDDEPAISELAEGLDVDVVPASVDDGRQFDVLINGKDRTWEIRS
                     NEVNGSVSEVSVYPGVRVAMTELQRKIASKGRIVVLGRDIGTVVLPDADLKIYLDASI
                     EVRAQRRLLEELSRGNTLDLEKTIEMLRHRDVIDSTREHAPLKAAEDAILINTDHLTI
                     EQVVEKIIGIVSDCSQA"
     gene            4232..>6404
                     /locus_tag="PK791_02195"
     CDS             4232..>6404
                     /locus_tag="PK791_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOH92155.1"
                     /translation="MKTEPFARLLDATKQTPQVPYGTWFLIIALLGVMVWLIHELRKP
                     AVKLDKKQKIALLLSIVALVPVNYLGIKANIYALDMTGFFSTPYFFIGLLQVVLIVFV
                     LGYMGLIAGFVMGLVTGIAQVILHKQDPGMIVVYSAIPVILAYLLHSPKVKRTSWRGT
                     RGWAFVVFCWIILVPVILLIMGTRALFLRLAFDWAVIRYCVFAWLSLLPAFGLAGLYY
                     WLCQKFFVQYWQLPTFVKQADSREELNTVIEQIRRLSNGTYDHELLGRPGHPRERQLY
                     QALETLRKNLLFKHETQARLLSLDPSHYSKEGYDLILSSVLKASLTRDASSARLLLLE
                     PGFDGEPANIRSRFGQGENTRLYAYLDGAILDKIGDREQLILTDIKVDQYFGLSAGSP
                     FPQSIIALPLSDKGHTHGVLWVAFEQKKWFAPDDIKFYQELAYRASVAMTTKQETQQL
                     QNDKVVYEAGMNAIPHPVFLLDNENIILFMNQSAKSLLGVDASLITSADGKHRLTHPK
                     ILERLKSDTSQQMRDSTLLFADGSSYDLEIYSLNTAETGEENAGKLVFLVNKNWLSQI
                     NQQRTEFVSNISHDLQAPIKMIKGHLKLLERMGNLNKEQQTYVKSIEDYTESMNRLVN
                     KVLTLEKLDDIEAINYTRFDFQQKTDDVIKLLLPSAQQKKISLEQDYTKMPTPFISAD
                     ATLIQQALYNLIDNAIKYSPMKSNVRVIAEKEASTLRVIVED"
BASE COUNT         1627 a         1204 c         1560 g         2013 t
ORIGIN      
        1 ggtcatggtg gcaggctagc cgtgttttgc gggatacaag cctgagcgga cattctattg
       61 aagttatcga cgagtttcca cagtttacat ttttgctggc ggacattcat ccgcatatgt
      121 tagggatgcc ttttgtgctg atagctattg ctttggcctt gaatttcatc cgtggtggag
      181 ctgaagggcg ctttaagatg gcaaaattgc tggttgccta tgaaccattg gctgtctttt
      241 ttgcggtttt gaccttgggc ggaatcgctt ttatgaatac ctgggacttt cctttttatc
      301 tggcattaat tgctgcgaca ttggtctacc gacgttatct ggaagatggc tggagtggta
      361 aaaggattat tgaatttttt gggattgtca tcatttgcgg atttttaagc atattgatct
      421 acctgccatt ttatttgagt tttgcatcac aagcgggtgg catattaccc agtttggcgt
      481 tttttacccc gggaataaac ttttgggtca tgtttgcgcc actactcgtg cctatctctt
      541 tttttctgct gtatcttgtt atcaagaaga aagctttcaa agagttaggt ctttcggtgc
      601 taattgtttt agctttgttt gcctttttgt ttgtattttc gtggggaatt ggctggctgg
      661 gaagccgaag cgaaacaaca tccgcgacaa ttcttggttt gcaaggcgcg cccgacagca
      721 gaacgctttt gacggaaagc atccgtttgc gtttgaaatc accaggaact gcgatcactc
      781 tatttgcgct tttatcgctt ggtatggcat cggttctgga caggaaaaag atacagaatg
      841 cagaaacaaa tagtgacgtg ataataaaag aacaaaccga gaaacccgtt catcttatat
      901 ttgtgctttt tcttatcatt ttaggcgcgt tgcttacatt ggcgccagaa tttgtgtatc
      961 tgcgggatca gtttagctgg cgcatgaaca cgatattcaa attctatttt caggcctgga
     1021 ttttatggtc actggcagcg agttacgcga ttgtttttct ctttggaaaa tcaggaacaa
     1081 gacgcgttcc aaacagctta tttgtgatgt tgatagcagg cttagttttt acgagtttcg
     1141 gactgagttt gaacgaacgc agcacatttc ctgctggatt tggtgcgtta agagcggatt
     1201 ggctggtgtt aggcattggt ttagtttttt tgctttggat tgttagccat ttgataagac
     1261 ctaatcttaa ggctgttttg gctgttgtca gtgtgcttgt tgtgctggcg ggtctgattt
     1321 atccgatttt ggaaatatgg aacaagacgg aaggttttca accgcgtttg gggttcactt
     1381 tggacggtaa acgtgtcttt tgggaaagct atcctgacgc gatgcgtgca gctgaatggc
     1441 ttgaaaccgc gccgatgggc gtgctggctg aagccgtagc agatcagggc ggttcgtata
     1501 cggcttacaa tctgatttca actttttcag gtatgccatc tgttttgggc tgggtcggac
     1561 acgaacatca atggcgtggg ggcggcgctg aagtgggaac gcgtcagcag gatttacgag
     1621 agttgtattc aacgaagaat atggacaggt ttaaagagat tattcttcag tataatatcc
     1681 gatacatcgt ttacggaaat tacgaacgtg atacctaccg tgttagcgag ccatacttta
     1741 gcagtgaatt gacccctgtg tttgaaagcg ataccgttac aatctatgag gtgagacagt
     1801 aaaatgcaag aaaagcgaca aatccctatc ttaaaagacc gacgagaact gatttggtta
     1861 ttgctgatta tttttcttgc tgcacttgta aggttgatta agcttggtgc gatgcctttc
     1921 aatgctgttg atgccagttt agctgccgat gctttggcta ttgcacgaaa tcagacactc
     1981 gggcaaagtg ctttgcctgc ctatacggga ctgacgggtg tgttatttta tattttgggt
     2041 agttcaaact tcgtcgcgcg attattgtcc gtttttgctg gtgtttcgct ggttgttttg
     2101 tcttttttgc tagctaaaca aaagagcttg cgtgtggcta ttatcttttc actggctttg
     2161 gcacttgacc cattgatggt taactggtcc agacagattg ccacgcccat gatcgctttg
     2221 gcaggcctgg catgggcagt ttgtctgttt gataaaaagc gctatatcat tgctggtttt
     2281 tgtttggcag tagcttttct tggtggctat tcattttggg tctttagctt acttgcgttg
     2341 gcgttatggg ttttcgcgcg tgtaataaag tttgaagtgc cattttttat aaacacattg
     2401 cctgaaaagt cagcttttct caaaacgtct ctctcttttt tgattagttt ggttttgata
     2461 tcaactgggt ttttcttaga acctactgga tttgggcaaa tcggggcaag cttgatggct
     2521 ttcctggggc tttttaagaa ggcttatcaa ttccctgtgt atcattcgtt ttttgttttg
     2581 attcggtttg cattattgcc acttttgggc gcgattattt ggctggtatc aagagggaac
     2641 ttatcgcgaa aagagaagtg gatgatagct ttctacttct tagtgtcagt tttgtttggt
     2701 ctgtttttct ccagaactga tgtaggctta agtatattaa tattaattcc gctatggtat
     2761 tttaccgccc gatacctgag cgagttccgc ttggatatta acgaaagact cagtttaaaa
     2821 attgtttgtt ttgtactctt tcttgtgatg tttatataca ttgccatcaa tttgcctaga
     2881 ttaatcgaag ctgaattcgg cagtccacaa ctcatttatg caggattagc tacattagct
     2941 ggcgtattac ttgtgcttat tgtgttttgg ctgggttctt tgtccttggg aatgaaacaa
     3001 agttcagcga tattagcaac agctttggtg cttgtgctta tttccagttc attagcacta
     3061 agctttaatt cacttgataa aaaccgtgat acaagccaag tcatggtgaa tgctgaaccg
     3121 attttattag caaatgaagc tgaactcacc gcaatcaatc cattccagga ttacggaaag
     3181 ttgaatttcg tggaagccag ttttttggtg gaagatattg cttatgattt gcgctggttt
     3241 acgcgagagt ttcaggctga gtcttctttg cgtgcgcctg agtttgtaat cacacatacc
     3301 gaaaatccgc cgaattacga tgaaccctat cgcggaatgc ccattgaact aagccgtaat
     3361 atgccgtgga cacaattaaa ttttgctggt tattttaatg ccattatcgc gcctgagcgg
     3421 gctatacaag ttgagaacgc aaacttatgg gcgctgacaa aactgtttac aggagcaatt
     3481 caataaatga aagacaaagc tgagaaaaca atacctgaaa ttattgccat tgatgggcct
     3541 gctgcatcgg gaaagtcaac ggtcggcgca ctcgttgctg aaaaattaga ttatgtttgg
     3601 cttgatacag gcatcatgta tcgcgctgtg gcttgtgaag ccttggaaac gggtgttccg
     3661 attgatgatg aaccagccat atcagaattg gcagaaggct tggatgtgga tgtggttcct
     3721 gccagtgtag acgatggccg acagtttgat gtattgatta atggcaagga tcgtacctgg
     3781 gagattcggt caaacgaagt caacggttcc gtgtctgaag tctcagttta cccgggtgtg
     3841 cgcgtagcca tgacggagtt gcagcggaaa attgcttcaa aaggacgaat tgtcgtgctt
     3901 ggtcgggata tcggaacagt cgttttgcca gacgcggact tgaaaatcta tcttgatgcg
     3961 tccatagaag tgcgcgcgca acgtcgcttg ctggaagagt tatccagggg taatacgctt
     4021 gatttagaaa aaacgattga aatgctgcga caccgcgatg tcattgactc aacgcgtgaa
     4081 cacgcacccc ttaaagctgc tgaagatgca attttgatta ataccgatca tcttacgatt
     4141 gagcaggtgg ttgagaaaat aatcggtatt gtatcagatt gctctcaagc ataagtgaaa
     4201 tttcaagagt catcatgcta taattttagt gatgaaaact gaaccgtttg caagattatt
     4261 agacgcgacg aaacaaacac cacaagttcc ctatggcaca tggtttctta tcattgcgct
     4321 gttgggtgtg atggtttggc ttatccatga gttaagaaaa cctgctgtaa aactggataa
     4381 aaagcaaaag atagcattgc ttttaagtat tgtggcactt gtgcctgtaa attatttggg
     4441 aatcaaagca aatatttatg cgctggatat gactggcttt ttcagcacgc catatttctt
     4501 tatcggcttg ttgcaagtag tcttaattgt ttttgtgctg ggatatatgg gactgattgc
     4561 aggctttgtg atggggctag tgacgggtat cgcacaggtt attctccaca agcaagatcc
     4621 tggcatgatt gtggtttatt ctgctattcc agttatcctg gcgtatttat tacattctcc
     4681 aaaagtcaaa aggacatcct ggcggggaac caggggctgg gcttttgttg ttttttgctg
     4741 gataattctg gtacctgtaa ttcttcttat aatgggtacc agagctttat ttttgcgtct
     4801 tgcctttgat tgggcggtta tcaggtactg tgtatttgcc tggctaagct tgttaccagc
     4861 ttttggtctt gctggccttt actattggct ttgtcaaaag ttttttgtgc aatattggca
     4921 actgccaacg ttcgtaaaac aagctgactc ccgtgaggag ttgaacacgg tcattgagca
     4981 gatacgacga ttgagtaatg gcacttacga tcacgagctt ctcggaagac ccggtcatcc
     5041 aagagaacga caactttacc aggcattgga aacgttgcgc aagaacctgt tgttcaagca
     5101 tgaaacacaa gcgcgactgt tgtccttaga cccatcacat tactcaaagg aaggctatga
     5161 cctaattttg tcatcggtgc ttaaagcgtc tttaacccgt gatgccagtt cagcgaggtt
     5221 acttttgctg gaacccggtt tcgatggtga acccgcaaat atccggtcac gctttgggca
     5281 aggcgagaat acgcgtttgt atgcttattt ggacggagcg atattagata aaataggcga
     5341 ccgcgaacaa cttatcctga cggatatcaa ggttgaccag tattttggtt tgagtgcggg
     5401 ttcgcctttt ccacaatcta tcattgcttt accgttgagc gacaaaggac acacgcacgg
     5461 tgttttatgg gtcgcctttg aacaaaagaa gtggtttgca cctgacgata tcaagttcta
     5521 tcaggaactg gcttatcgtg cctctgttgc catgacgacc aagcaggaaa ctcaacagtt
     5581 gcaaaatgac aaggtcgttt atgaagccgg catgaacgcc atcccacatc ccgtgttttt
     5641 actagacaat gaaaatatca ttttgtttat gaatcaatct gccaaaagtc tgttaggtgt
     5701 tgatgcaagc ttgataacga gtgctgatgg aaaacaccgg ttgactcatc caaaaatatt
     5761 agaacgcctg aagagtgata ctagccagca aatgcgcgac agtaccttac tgttcgcaga
     5821 cggatccagt tatgacctgg aaatctattc cctgaatact gccgaaactg gtgaagaaaa
     5881 tgctgggaag ctggtctttt tggtgaataa aaactggctg tcgcaaataa atcagcaacg
     5941 aactgaattt gtaagtaata tcagtcatga tttacaagcg ccgataaaga tgattaaggg
     6001 gcatttgaag cttttggagc gcatgggtaa tttgaacaaa gagcagcaaa catacgtgaa
     6061 atcgattgag gactataccg aaagtatgaa tcgattggta aacaaggtct tgactctgga
     6121 aaagctggat gacatcgagg cgataaatta cacacgcttt gattttcaac aaaagactga
     6181 tgatgttatc aagctattgc ttccaagcgc acaacagaag aaaataagtc ttgagcaaga
     6241 ttatacaaaa atgccaacgc catttatttc tgcagacgcg acccttattc agcaagcttt
     6301 gtataatctt attgataacg cgattaaata tagtccgatg aagagtaatg tgcgggtgat
     6361 tgctgagaaa gaagcatcaa ccttgcgtgt cattgttgag gacg
//
LOCUS       DAOSFC010000049         6400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10626_length_6400_cov_5.708589, whole genome shotgun sequence.
ACCESSION   DAOSFC010000049 DAOSFC010000000
VERSION     DAOSFC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10626_length_6400_cov_5.708589"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PK791_02200"
     CDS             complement(<1..216)
                     /locus_tag="PK791_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOH92156.1"
                     /translation="MKILMIYPEFPDTFWSFKHALSFIRKKASSPPLGLLTIAGLFPQ
                     SWEKRLVDLNVEKLRDKDIQWADMVFIS"
     gene            complement(318..1121)
                     /locus_tag="PK791_02205"
     CDS             complement(318..1121)
                     /locus_tag="PK791_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH92157.1"
                     /translation="MARKDDRRIQRTRKLLRESMLALILDRGYDDISIQDVTDHANLG
                     RATFYLHYREKDELFRDVLLSMAEEFLATLPKQDKKSDQTLNTKKLEMLFSYVEDRFD
                     FYRIVNMGRSAILTSAIVAELIKENFKVFSTTGMETYESITGIPTDFLLSFNASTLKS
                     TLFWWLENDMPYTAAEMAEMYTKVSFFTNFQILRAGKDMELQKAESNDPGKASKQEAQ
                     NQDKAQSDTKENAPKKENKKASKKKDANKSEELEQEEPSPESEAPESDQ"
     gene            1329..2306
                     /locus_tag="PK791_02210"
     CDS             1329..2306
                     /locus_tag="PK791_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH92158.1"
                     /translation="MLQAQKAGAYANYILGMNITAEETILVYGNQSGIISERISFKPE
                     VNQSFQNFLALISQHSDKLLNLARAQHLPLPERVSVSIAGNYDAQQGILISSRDFPAW
                     KNEPLKSQLQLLFNLPVFVEKNAVAGAQAEKLFGAAQDLRDFVFIDLGHNLSMAFSAQ
                     GKIFSPSNPHAGAVGRVRLCQESDASGEGPLTLNEQCSSVGMVRQALATQASHWDPQV
                     TVYQIINAARNDDPYAIEILGKAGASLGHHLAPYIHLLRPEAIILGFPASLAGEIFSE
                     AVFKAAQQSTGLGLDEMPRVIPSPLGTRLPELQAIAPSIHATRYPADSK"
     gene            complement(2372..3226)
                     /locus_tag="PK791_02215"
     CDS             complement(2372..3226)
                     /locus_tag="PK791_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOH92159.1"
                     /translation="MTKEQTRTENDKRTNDILLGPLERPALAFFAKHMPMWVNSDMLT
                     IFALFGAAMIFLAYFLAGQAGDLKGNGYLFLANLGLFIHWFGDSLDGTLARYRHHERP
                     NYGYYTDHALDGLASVMIFMGIGLSGLADFKVTMFALVTWLLVMMQVFLKTHATGTFE
                     MTSAKIGPTEIRVLLAILNTVIYFTGAGQPLAVLELRGLTISFTPGTIVLAVVSIVFL
                     VYYIYQVVTTGSKLSAEDLEALKVKNAERARKGIVSQMELDRREKEKKLASQQKPKTL
                     LDLFSKPD"
     gene            complement(3256..4149)
                     /locus_tag="PK791_02220"
     CDS             complement(3256..4149)
                     /locus_tag="PK791_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH92160.1"
                     /translation="MKLKDFLQDEGLVRLGKGLGKNLSLRGIRRLANFVSGILNRYPK
                     NQMRRVIKGNLSVVLDEEANSDIINALSKEVIRNLMWTHADYFYLYQHPEESKSAIEF
                     SSNALEMIEDVRERRQPTVICGPHYGNFDLLGLSLARKSVPMMVLSVPNPQGTYSAQN
                     EMRNKSGMNVRPIDMKAIRDAKKFLLEGNAVVTGIDRPVEDPKNAKYKPLFFGKPAAL
                     PVFYTRFGLEEGVKLRVAYCKRKSDGRYLVDCSAPVEMRQYDNLKEEYEKNAQATLRE
                     AEKIIRKEPEHWLMLHPVWEN"
     gene            complement(4239..5066)
                     /locus_tag="PK791_02225"
     CDS             complement(4239..5066)
                     /locus_tag="PK791_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOH92161.1"
                     /translation="MYNTLAEIFSYEFMQNALLAGVLVSLVSGIIGTLVVLNRMVGIS
                     GGIAHAAYGGVGIASYFGFDPVLGAVLFSLLSSVTMGVAHRKLRERSDTMIGVMWAVG
                     MATGIIFVSLTPGYRANLMSYLFGSILAVSKQDIWWMAGIALATVLFVVLFYRQLLAI
                     SFDEEFAIVRNLPVTVLSVLLLIMTGLTVVVAMRVVGLIMVIALLTIPSAIASMFFKE
                     MRVIMLVAVGLGIVFISAGLVISFLSNLPAAAVIILLAGLVYFLAFVIKRILLKTKT"
     gene            complement(5059..5892)
                     /locus_tag="PK791_02230"
     CDS             complement(5059..5892)
                     /locus_tag="PK791_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92162.1"
                     /translation="MTESIQAPTKSILKIEDMWAGYDGEAVLEDIQFEMFAGDYVGII
                     GPNGGGKTTLIKVILGLLPVMRGSVSVLGQSPVQARDRLGYVPQVSRTDRDFPISVWD
                     VVRMGREKAGFFGVRSLSAQDKQAIERALGQADVLDLAKRSFNELSGGQRQRVLIARA
                     LATNPEILVLDEPTANVDGQSTARLYDLLAELNQSLSILLVSHDLMAISTHVKTIACV
                     NRKMVYHNEKVLTQEMMDLGYECPVELLAHGVPHRVLAHHDHDHLSSNTSHKQEDNQD
                     V"
     gene            complement(5918..>6400)
                     /locus_tag="PK791_02235"
     CDS             complement(5918..>6400)
                     /locus_tag="PK791_02235"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOH92163.1"
                     /translation="DPHVWFSPDNARQIAENIANALIEQDPQNESTYTNNLESVLEEI
                     ANTDEEITSILQDISRRHFMIVHPAWGYFAHAYGLHMIPVEIGGNEPGPADMAKILEA
                     VKDYQISVLFVEKGSNMSLAQSIARQAGISEIVEWDPMAYEWAENMLSLGRDLQNALR
                     "
BASE COUNT         1664 a         1617 c         1388 g         1731 t
ORIGIN      
        1 actgataaag accatatccg cccattgaat gtctttgtcg cgaagttttt cgacgttgag
       61 atccaccagg cgtttttccc agctttgcgg aaaaagcccc gcgatggtta aaagccctag
      121 gggaggtgag gacgcttttt tacgaatgaa gctcaaagcg tgcttgaagc tccaaaaagt
      181 atcgggaaat tcggggtaaa tcatcaagat tttcatgctt aatcctttat tttaaatatt
      241 aactaatctc gcgtggctat atgccttgcg gtggtaaagt tcttcaatcc ccgtggctta
      301 tgtgccttat ttaattttta ctggtctgat tctggtgctt cgctttcagg cgatggttcc
      361 tcctgctcaa gctcctcaga tttgttagcg tcttttttct ttgacgcctt tttgttttcc
      421 tttttgggcg cgttttcttt ggtatcagac tgcgctttat cttggttttg ggcttcttgt
      481 ttggatgctt tgcctgggtc gttggattcg gctttttgca gttccatatc tttcccagcc
      541 cgcaaaattt ggaaatttgt aaagaaactt acttttgtgt acatctcggc catctcggca
      601 gcggtgtagg gcatatcgtt ttcgagccac caaaagagtg tgcttttcaa tgtgctggcg
      661 ttaaaactta atagaaaatc tgtaggtatg ccggtgatgc tttcgtaggt ttccattcca
      721 gttgttgaaa agaccttgaa gttctcttta atcaattcag ctacaatcgc ggatgtcaat
      781 atcgcgctac gccccatatt gacgatgcga taaaaatcaa aacgatcttc aacgtaactg
      841 aaaagcatct ccagtttctt tgtgttgagt gtctgatcgc ttttcttatc ttgcttgggc
      901 aaggttgcta aaaactcttc agccatgctt agaagcacat cacgaaagag ctcgtctttt
      961 tcgcgatagt gcagatagaa agttgcccga cctaagttag catggtcggt gacatcctga
     1021 atgctgatgt cgtcataccc tctgtcaaga ataagcgcaa gcatactctc gcgcagcaat
     1081 tttcgtgttc tttgtattct tcgatcgtcc ttacgtgcca ttgttttctc ctttgtcgcc
     1141 agataactct ctacacacag tccactagta gacagcagtt aatttatccg tctattattt
     1201 tacgaataaa tttcagcttg tcaagtagta tgcatcaaat ttcaaggtga ttaatgtaac
     1261 gaatatttgc gttaaaaaaa gataataaaa aaacgctcta atatgggatt acggctataa
     1321 taattactat gttacaagca caaaaagccg gtgcctacgc taattacatc cttggcatga
     1381 atatcaccgc tgaagaaact atccttgttt acggcaatca gtctggcatc atcagcgaac
     1441 gcatcagttt taaacctgag gtcaatcaaa gcttccaaaa ctttttggcg cttatctccc
     1501 agcacagcga caagctgctc aacctcgccc gcgcccaaca tctccctttg cccgaacgcg
     1561 tttccgtatc aattgccggt aactatgacg cccagcaagg tatcttgatt agttcgcgcg
     1621 attttcctgc ctggaaaaac gaaccattaa aatcacagtt gcaactgctt tttaacctgc
     1681 ccgtttttgt tgaaaaaaat gctgttgccg gcgcacaggc agaaaaacta tttggcgctg
     1741 cccaagacct gcgcgatttt gttttcatcg acctgggaca caaccttagt atggccttta
     1801 gtgcccaggg taagatattt agtcccagca acccccatgc cggggcagtt ggccgtgtca
     1861 ggctctgtca ggaaagtgat gcgagtggcg aaggcccact tacgctcaac gaacaatgca
     1921 gctctgttgg catggttcgg caagcccttg cgacacaagc cagccactgg gacccacagg
     1981 taactgtcta ccaaattatc aacgctgccc gcaacgacga cccctacgcc atagaaattc
     2041 ttggcaaagc gggagcaagc ctggggcatc atttggcgcc atatatccat ttattgcgac
     2101 cggaggctat cattttgggc tttcctgctt cgttggctgg tgaaatcttt agcgaagccg
     2161 tattcaaagc cgcgcagcaa agtactggct tggggcttga cgaaatgccc agagttatac
     2221 cttccccttt aggcacgcgt ttacctgaac ttcaggctat tgctccatca atacacgcaa
     2281 cacgctatcc agcagatagc aaataaatga actgctaaaa aataaaggct acctgaaggc
     2341 agcctttatt tctaaacaaa ctaatcatgg attaatcagg cttgctgaat aaatccagta
     2401 aggttttcgg tttttgttgg ctggcgagtt tcttttcttt ttcgcggcgg tcaagttcca
     2461 tttggctcac gatgcccttc cgggcgcgtt ccgcgttctt tacttttaag gcttcaaggt
     2521 cttcggccga gagtttgctt ccagtggtaa ccacctggta aatgtagtaa accaaaaata
     2581 caatggaaac cacagccaga acaatcgtgc cgggtgtgaa actgatggtc aaacctcgca
     2641 actcaaggac tgccaatggc tgacccgctc cggtgaaata aattaccgtg ttcaagattg
     2701 ccaggagtac acgaatttct gttgggccga tcttggcact tgtcatctca aacgtgccgg
     2761 tggcatgtgt tttcaaaaag acttgcatca tcaccaaaag ccaggtcact aaagcgaaca
     2821 tggtgacctt aaaatcagca agtccactta agccgatacc cataaagatc attaccgacg
     2881 ccaaaccgtc cagggcgtgg tcggtgtagt atccatagtt ggggcgttcg tgatggcggt
     2941 aacgtgccag ggtgccatcc aggctgtcac caaaccaatg gataaacaag ccaagattag
     3001 ccaggaacaa atagccattg cctttcaggt cgccggcttg acccgccaaa aaataggcca
     3061 aaaagatcat tgccgcgcca aaaagcgcaa agatagttaa catgtcggaa ttgacccaca
     3121 tgggcatatg tttggcaaag aaagctaagg ccggtctttc cagcggacct aataaaatat
     3181 catttgtgcg tttatcgttt tcagttcttg tttgttcttt tgtcatctta caccagcttt
     3241 cgtttcgaat cgcgtttaat tttcccaaac cggatgtaac atcaaccaat gttcaggctc
     3301 tttgcggatg attttctccg cttctctgag ggtcgcttgc gcgtttttct cgtattcttc
     3361 tttaagatta tcatattggc gcatttcaac gggagcgcta caatctacaa gatatcgccc
     3421 gtccgatttg cgcttgcaat aggctacccg taatttaacg ccttcttcca gaccgaatcg
     3481 cgtataaaaa acaggcaatg ccgcaggttt accaaaaaac aaaggcttgt acttggcatt
     3541 ttttggatct tcaaccggtc tgtcaatacc agtaacaacg gcgttgcctt ccagcaggaa
     3601 cttttttgcg tcgcgaatgg ctttcatgtc aatcggtctg acattcatgc cggatttatt
     3661 gcgcatttca ttttgcgcgc tatacgtgcc ctgcggattc ggcacgctta gaaccatcat
     3721 cggcacactc tttctggcga gcgaaagtcc taaaaggtca aagtttccgt aatgtggtcc
     3781 gcaaatgacc gtcggctggc gacgttcccg gacgtcttca atcatctcca gcgcgttgga
     3841 cgaaaattcg atggctgact tactctcttc agggtgctga tagagataaa aatagtctgc
     3901 gtgtgtccac atcaggttac ggatgacttc tttgctgagt gcgttaatta tatcagaatt
     3961 cgcttcttcg tctaaaacaa ccgaaaggtt accctttatc acccttcgca tttgattttt
     4021 ggggtacctg ttaagtatgc cggagacaaa atttgccaac cgacgaatgc ctcgcaaaga
     4081 gagattcttt cccaaaccct tgccgagacg caccaaacct tcatcctgca aaaagtcttt
     4141 cagtttcata gcgcaattcc actctatata attgtaaaca ccatgtcaag gatttggtta
     4201 tttaatttta cataaaaatc acaaagagat gacaattgtc atgttttcgt tttaagtaga
     4261 atgcgtttaa tgacaaatgc caggaaataa accaagccag ccagcaaaat aattaccgcg
     4321 gctgcaggga ggtttgaaag gaaagatatc accaaaccag cggaaataaa cacaataccc
     4381 aaacccaccg cgaccagcat aatcacccgc atttctttga agaacatact ggcaatggct
     4441 gatggtatcg ttaaaagcgc gataaccata atcaagccca ccacgcgcat cgcgacaacc
     4501 acggtaagac cagtcatgat gagcaataag accgatagta ccgtaaccgg cagattacgc
     4561 acaattgcaa attcttcatc aaaagaaatg gccaggagtt gccggtaaaa aagcaccaca
     4621 aacagcaccg tcgccagcgc gatacccgcc atccaccaaa tatcctgttt tgaaactgcc
     4681 aaaatactgc cgaacaaata cgacatcaaa ttcgcccggt atcccggcgt gagactcaca
     4741 aaaatgatac ctgtcgccat gccaacagcc cacataacgc ctatcatggt gtcggaacgt
     4801 tcgcgcagtt tgcgatgcgc cacgcccatg gtcaccgatg atagtaagct aaacaaaact
     4861 gcgcccagca ccgggtcaaa accaaagtaa ctggcaatgc ccacgccacc ataagccgcg
     4921 tgcgcgatgc cgccactgat tcctaccatg cggttcagca ccaccaaagt gccgataatg
     4981 ccactgacaa ggcttaccag tacccctgcc aaaagggcgt tttgcataaa ttcataagag
     5041 aagatttcag ctaaggtatt atacatcttg attgtcctct tgcttatggc ttgtatttga
     5101 tgaaaggtgg tcatggtcat gatgcgccag cacgcgatgg ggtacgccat gcgccaaaag
     5161 ctccaccgga cactcgtatc ccaggtccat catttcctgg gtgagaactt tttcgttgtg
     5221 atagaccatt tttcgattca cacaggcaat cgtctttacg tgcgtggaga ttgccatcag
     5281 atcatgcgac accagcaaaa tgctcaggct ttgattgagt tcggcgagta agtcataaag
     5341 gcgtgcggtg gattgcccat cgacgtttgc ggtgggttcg tcaagcacca gaatctcggg
     5401 atttgtggcc agggcgcgcg cgataagcac gcgctgacgt tgcccgcccg aaagttcatt
     5461 gaaagaccgc tttgccaaat caagcacatc agcctgacca agcgcgcgtt caatcgcttg
     5521 cttgtcttgc gcggacaaag acctgacgcc aaaaaagccc gccttttctc ggcccatgcg
     5581 caccacatcc caaacactaa tcgggaagtc tctgtccgtg cgcgagacct gcggcacata
     5641 acccaaacgg tcgcgcgcct gcacaggact ttgtcccagc acgctcacac ttccgcgcat
     5701 aacgggaagc aagcctaaaa tgaccttgat gagtgttgtc ttgcccccgc catttggccc
     5761 gatgatgccc acatagtcgc ccgcgaacat ctcaaactgg atgtcttcca aaacagcttc
     5821 gccatcatag cccgcccaca tatcttctat ttttaaaatg cttttcgttg gtgcctgtat
     5881 cgattctgtc acttttcaat acctttaaaa cttcccatta gcgcaaggcg ttttgcaaat
     5941 ctctgcccag agaaagcata ttttcagccc actcgtatgc cataggatcc cattcgacga
     6001 tttcgctgat acctgcctgc ctggcaatcg actgcgccaa actcatattg ctgccttttt
     6061 caacaaaaag cacgctgatt tgatagtctt tgacggcttc cagaattttc gccatgtccg
     6121 cgggaccggg ttcattgcca ccgatttcca ccggaatcat gtgcaggccg taggcgtgcg
     6181 cgaaataccc ccaggcagga tgcacgatca taaaatgccg tcgcgagata tcttgcaaga
     6241 tgcttgttat ttcttcatca gtatttgcga tttcttcaag cacgctttct aaattgttag
     6301 tgtaggtact ttcgttttgg gggtcttgtt caatcagcgc attggcaata ttttccgcga
     6361 tttgccgggc attgtcaggc gaaaaccaaa cgtgcgggtc
//
LOCUS       DAOSFC010000050         6308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10865_length_6308_cov_5.313609, whole genome shotgun sequence.
ACCESSION   DAOSFC010000050 DAOSFC010000000
VERSION     DAOSFC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10865_length_6308_cov_5.313609"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PK791_02240"
     CDS             <1..722
                     /locus_tag="PK791_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92164.1"
                     /translation="WAAPQPTDDDYQIAFSVDVDGNPIYTADMTPEDKYAAAREAALG
                     FLEAAGFTIEDGKAVEAPEGAKLNYEVIVPGGGTGDHPAFAILTNAEADLAAIGITLN
                     INDPANANILWDALDGGQQEMWTAAWGSTIDPDMYQVYHSSNVVGEGGTDSNHYHIRD
                     AQLDQFIMDARMSDEQNYRKAVYKQALDVVIDWAVEIPTYQRQNANIFSTQRLNIDTL
                     TPDITTFWGWMNDIELLEMLP"
     gene            847..1836
                     /locus_tag="PK791_02245"
     CDS             847..1836
                     /locus_tag="PK791_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH92165.1"
                     /translation="MSNFIIRRVFLGLFIVFFGAMIVYGVIRSLPSSYVETVARQRAA
                     NPTSLRTYQEWLDELNQVYHLDVGIIPGYFGWVKNAIRGDFGVSWIYNVPVTQKFKEV
                     IPYSIFLNIITFAVQIIVSIPLGILAARKQYSGVDYAITVFALAGLSLPTFFLATLLK
                     YVFSVKLGWFPLYGLTSRFHNQMDRTGQLLDMAKNMTLPIITLMMLSIGGLMRYTRTN
                     MLEVLNSDYIRTARAKGLSEKVVINKHAFRNTLIPLVSYMSTLLPAMFGGSMITETMY
                     QIPGIGYISFGAMQQGDIPFAMFYTTFLILLTQISLLIADIMYAVVDPRVRVN"
     gene            1850..3622
                     /locus_tag="PK791_02250"
     CDS             1850..3622
                     /locus_tag="PK791_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH92166.1"
                     /translation="MSDENKKNLEIDEPTDAQIGSDGLPMTTTEEMALDDQRRIKVLS
                     PGMLVFKRFIRNKLAVVGLIILAIMFTFSFIGPLFSPYTQTQVFTGMSTMSREYAGAI
                     YNLEERYTVADGETFNSADRAAYLLAKGRGSSTFNSANGEFYYEEILPGFNRIAKLDV
                     VAKTDPLGKLPTDDPAISEDFIKAYEANKKAGSFVFEDVLYIITGPKVKTISTDKDIA
                     VASTFVFDAYNKADDKFVQSHLFKLAVANAIAEEADTLELENREFTIVMDERSGTISD
                     ETGKHFAQISDIIVNPLDSSAFLSVDFKETVRETMNARKNEFYFTDENGIETHYKIVI
                     VNQNYSIMKDMPTQLVRTYEFPSKEHWLGLDNNGMDLLTRLMYGGRVSLMVGFVVIII
                     EMLIGVIFGGISGYFGGTVDTLMMRFVDLFNSIPFYPIVIIFGSVMDTLQVDSTTRIF
                     LLMAILGLLGWTGIARVVRGQILSLREQDFMVATEATGIRTSRRIFRHLIPNVMPLLI
                     VSATAGLGGIIITEATLGFLGLGVKYPLASWGSIINNASSAHVMQSYWFMWFPAGMLI
                     LLTVLGFNFVGDGLRDAYDPKMKR"
     gene            3632..4840
                     /locus_tag="PK791_02255"
     CDS             3632..4840
                     /locus_tag="PK791_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92167.1"
                     /translation="MTDEKIDLKQFDTTEAEEDRVKESIHLSARESARITRENRRITN
                     AIEKHRNRRNVDPSEYLTEMVNPDNVVEFDDVHTYFFTDIGTVRAVDGVTFDVPVGKT
                     VGVVGESGCGKSVTALSLMQLVQRPQGQTVKGEIRLNTGDKVYNIVETPTEIMETIRG
                     AQVSMIFQEPMTSLNPVFKAGHQIDEVIALHNPEMSKEAVKQKSIDMIEMVGIANAEG
                     VYEMYPHELSGGMRQRVMIAIALACNPRLIIADEPTTALDVTIQAQILDLLRQLKDRI
                     NSSIMLITHDLGVVAEMADYVVVMYAGRIVEKGTAHDVFLHPQHPYTVGLMASKPVIN
                     KEVDRLYSIPGAVPNPINMPNYCYFRDRCNKRKPICNGDYPPEIYTSDTHMVACYLYK
                     DQNEEANNGK"
     gene            4830..5858
                     /locus_tag="PK791_02260"
     CDS             4830..5858
                     /locus_tag="PK791_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH92168.1"
                     /translation="MENENLSNELIHDDEYIAEVYRLKQYFPIKAGLFQRVVGHVKAV
                     DGVTFRIRKGTTLGLVGESGCGKTTVGKTMLRLNTATSGKVLFDGVDVFALDREGIRD
                     LRPRMQMIFQDPYSTLSPRMPISEIVGEAVREHNIVPRDEFDDYIDHVMLNCGLQPYH
                     KDRYPHEFSGGQRQRICIARSLALKPDFIVCDEPVSALDVSIQAQIINLLEDLQEQNK
                     MAYLFISHDLSVVQHISDYIGVMYLGDIVEYAKKEYLFENPLHPYTQALLSAVPIPDP
                     TLKMKRIILEGSIPSPANPPSGCKFHTRCPRAMDICKQEVPPVIELKLDHEVACHLYA
                     GQGKVLDL"
     gene            5860..6135
                     /locus_tag="PK791_02265"
     CDS             5860..6135
                     /locus_tag="PK791_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92169.1"
                     /translation="MLKEYPDDDGRVICDMNVEGMRWYNRNLSKVDDYRKSREKPAPY
                     HSAGEVMTRRETVRYTFYAVLAGLTIVAVFATAWILLWLFSAYVWFK"
BASE COUNT         1665 a         1472 c         1450 g         1721 t
ORIGIN      
        1 gctgggctgc tcctcaaccc accgatgacg attaccaaat cgctttctcg gttgacgttg
       61 atggcaaccc catctacacc gctgatatga cacctgaaga taaatacgcg gctgcccgcg
      121 aggctgcttt gggcttcctc gaagctgctg gcttcaccat tgaagatggc aaagcagttg
      181 aagctcctga aggcgccaag ctgaactacg aagttatcgt tcctggtggc ggtactggtg
      241 atcaccctgc tttcgcaatc ctgaccaatg ccgaagctga cttagcagca attggtatca
      301 cattgaacat caacgatcct gccaatgcca acatcctttg ggacgcgctg gatggcggac
      361 aacaagaaat gtggactgct gcctggggct caaccattga tcctgatatg taccaggtct
      421 atcacagcag caacgttgtt ggcgaaggtg gaaccgactc caaccactat cacatccgcg
      481 atgctcaact tgaccaattc attatggacg ctcgcatgtc cgatgaacag aactatcgca
      541 aggctgttta caagcaggct ttggatgtgg ttattgactg ggcagtggaa atccccacct
      601 accagcgaca aaacgctaat atctttagca cccaacgctt gaacatcgac accctcactc
      661 cagatatcac aaccttctgg ggttggatga atgatatcga gcttcttgaa atgctcccct
      721 agttcaactg tttgaactgg gtgagtcaga acgctgacta gcactgtgag cccacctaag
      781 gtgggctcac attctatcaa ctcagaacga gtacgctcgt ttattatttg aaggaagtga
      841 aacgctatga gtaatttcat aattcgaaga gttttccttg gtctgttcat tgtcttcttt
      901 ggagcaatga ttgtatatgg tgtaatccgc agtttgccca gttcttatgt tgagacagtt
      961 gccagacaac gtgcagctaa tccaacgagc ttgcgaactt atcaggaatg gttagatgag
     1021 ttaaaccaag tctatcacct tgatgtgggc attattcctg gttactttgg atgggtcaaa
     1081 aatgctatcc gtggtgattt tggtgtttcc tggatttaca atgtgcctgt aacacaaaag
     1141 tttaaggagg ttattcctta ctcaatcttc ttgaacatta tcacctttgc ggtgcaaatt
     1201 attgtttcga ttccgctggg tattctggcg gcacgcaagc aatattctgg tgtggactac
     1261 gcaattaccg tttttgctct ggcagggctt tcgttaccga cctttttcct ggcgaccttg
     1321 ctaaagtatg tgtttagtgt caagttaggc tggttcccat tatatggctt gacaagccga
     1381 ttccataatc agatggatcg aacagggcag ctcttggata tggcaaaaaa tatgaccttg
     1441 ccaattatta ccttaatgat gctttccatt ggtggtttga tgcgttatac ccgtacgaac
     1501 atgcttgagg tactcaactc cgattacatc cgcacagcac gggcaaaagg tctttctgag
     1561 aaggtggtta tcaacaaaca cgctttcaga aacacgctca ttccattggt aagctacatg
     1621 agcaccctgt tacctgccat gtttggtggc tccatgatca ctgaaaccat gtatcaaatt
     1681 cctggtatcg gttatatttc atttggtgct atgcaacagg gcgacatacc gtttgcaatg
     1741 ttctatacca catttttgat tctactaact cagattagtc tgcttattgc agatattatg
     1801 tatgcagtgg tagacccccg cgtgagagta aactaaaggt aggcgaaaca tgagtgatga
     1861 aaacaaaaaa aatctggaaa tcgatgaacc tactgacgca caaattggca gcgacggatt
     1921 gccgatgacc acaactgaag aaatggcact tgatgaccaa cgccgtatta aggttttatc
     1981 acctggcatg ttggtgttca agcgtttcat tcgcaacaaa ctcgctgttg tgggccttat
     2041 catcctggcg attatgttca ctttttcctt cataggcccg ctgttttccc catataccca
     2101 aactcaggtt tttactggta tgtcaacgat gtctcgtgaa tatgccggtg caatttataa
     2161 ccttgaggag cggtacactg tggctgatgg tgaaactttc aattccgctg atcgcgcagc
     2221 ctatctgtta gccaagggac ggggaagttc gacctttaac tcagcgaatg gtgagttcta
     2281 ttatgaagaa atactgccag gctttaaccg catcgccaaa ctggacgttg tggcaaaaac
     2341 agacccctta ggcaaactgc caaccgatga cccagctatt tctgaggact ttataaaagc
     2401 ctacgaagcg aacaaaaaag ctggaagttt tgtttttgaa gatgtcttgt acatcatcac
     2461 aggaccaaaa gttaaaacaa tctcaactga caaagatatt gctgtagctt caacatttgt
     2521 ttttgatgca tataacaaag ctgacgacaa gtttgtgcag tcacatcttt ttaagcttgc
     2581 agtggcaaat gctattgctg aagaagccga tacccttgag ttggaaaaca gagaatttac
     2641 gattgtaatg gatgaaagaa gcggtacgat tagtgacgaa accggcaaac actttgctca
     2701 aatttccgac atcatcgtta atcccttgga ttcaagtgct tttctaagtg ttgatttcaa
     2761 agaaaccgtt cgtgaaacaa tgaacgccag aaagaacgaa ttctacttta cagatgagaa
     2821 tggaattgaa actcactata agatagttat cgtcaaccaa aactacagca ttatgaaaga
     2881 catgcccaca caattagtgc gaacctatga attccccagt aaagaacact ggttagggct
     2941 tgataacaac ggtatggacc tgctcacccg tctgatgtac ggtggacgtg tttctctgat
     3001 ggtgggtttc gtggttatta tcattgagat gcttatcggt gtcatcttcg gcggtatctc
     3061 tggttacttt ggtggcacgg ttgataccct gatgatgcgc tttgtagact tgtttaatag
     3121 tattcctttc tacccgattg tgattatttt tgggtctgta atggatactt tgcaggttga
     3181 ttcaaccaca cgtatattct tattgatggc tatattgggt ttgcttggtt ggactggtat
     3241 tgcccgggtt gttcgtgggc aaattctgag cctgcgcgaa caagacttta tggttgctac
     3301 cgaagcgacc ggtatcagaa ccagccgccg cattttccgc caccttatcc cgaacgtcat
     3361 gccactgctg attgtttccg ccactgctgg tttaggtggc atcatcatca cagaggcaac
     3421 gcttggcttc ctgggattag gcgtaaaata tcccttagca tcttggggta gtatcatcaa
     3481 taatgcatca agtgcccatg ttatgcagtc gtactggttt atgtggttcc ctgctggtat
     3541 gctgattttg ctgaccgtgt tgggcttcaa ctttgttggc gacggcttgc gcgatgctta
     3601 cgatccaaag atgaaacgtt aggagtttta gatgactgac gaaaaaatag atttaaaaca
     3661 atttgacaca acagaggctg aagaagatcg cgtaaaggag agtatccacc ttagcgccag
     3721 agaatcagcc cgaattacgc gcgagaatcg ccgcattacc aatgccatcg aaaaacaccg
     3781 caaccgtcgt aatgttgacc cttcagagta tcttactgaa atggtcaacc cggataatgt
     3841 ggttgagttt gacgacgtac acacttattt cttcaccgat atcggtactg ttcgcgctgt
     3901 tgatggcgta acttttgatg ttcctgttgg taaaacagta ggtgtggtag gtgaatcagg
     3961 ctgtggcaag tccgttaccg cgctttccct gatgcagttg gtgcaacgcc cacaaggcca
     4021 gaccgtaaaa ggcgaaattc gccttaacac cggcgataag gtctacaata ttgttgagac
     4081 cccaactgag attatggaaa ccattcgtgg tgcgcaagtt tcgatgatct tccaggagcc
     4141 aatgaccagt ttgaaccctg tctttaaagc cggacatcaa attgacgaag ttatcgcatt
     4201 acacaatcct gaaatgagca aagaagctgt caagcaaaaa tcaatcgaca tgatcgaaat
     4261 ggttggtatc gccaacgctg agggtgtcta tgaaatgtac ccccacgaac tctccggtgg
     4321 tatgcgccag cgtgtcatga ttgctatcgc tttggcttgt aacccgcgct tgattattgc
     4381 tgacgaacca acaaccgccc tggacgtgac cattcaggca caaattttgg acttgttgcg
     4441 ccagctcaaa gaccgcatca actccagtat tatgctcatt acccacgacc tgggtgtggt
     4501 agccgaaatg gcagattatg ttgtggttat gtatgccgga cgcatcgttg aaaaaggcac
     4561 ggcgcatgac gtgttcttgc atccacagca tccttatact gtcgggttga tggcatctaa
     4621 accggttatc aacaaagaag ttgaccgctt atacagcatc ccgggtgcag tgcctaaccc
     4681 catcaacatg cccaactact gctactttag agaccgatgt aataaacgca agcccatttg
     4741 taatggcgac tacccacctg aaatttacac atctgatacg cacatggtag cttgctatct
     4801 ctacaaagat caaaacgaag aggccaacaa tggaaaatga aaatctttct aacgaactga
     4861 ttcatgacga cgaatatatt gccgaggttt atcgcctaaa gcaatatttc ccaatcaaag
     4921 ctggtttgtt ccagcgagtg gttggccatg tgaaggctgt agatggcgta acttttagaa
     4981 ttcgaaaagg cacaacactt ggcttggtgg gtgagtccgg ttgcggaaaa accactgttg
     5041 gcaaaacgat gttgcgactc aataccgcta catcaggtaa agttctcttt gatggcgttg
     5101 atgtctttgc cttggatcgc gaaggcattc gtgacttgcg cccccgcatg cagatgattt
     5161 tccaggaccc ctattcgact ctttcaccgc gtatgcccat tagcgagatt gttggcgaag
     5221 ctgtccgcga gcacaacatt gtgccacggg atgaattcga cgattatatc gaccatgtca
     5281 tgctcaactg cggtttgcaa ccctatcaca aagaccgcta cccacacgag ttctccggtg
     5341 gtcagcgcca gcgcatttgt atcgcgcgtt cactcgcttt gaaacccgac ttcatcgttt
     5401 gcgacgagcc ggtctcagcc ctggatgtct ccattcaggc gcagatcatc aacttgcttg
     5461 aagatctgca agagcaaaac aaaatggctt atctctttat ctcccacgac ctttcggttg
     5521 tccaacacat ctccgattac atcggcgtga tgtacctggg cgatattgtg gaatatgcca
     5581 agaaagaata tctctttgag aacccgctcc atccttacac gcaggctttg cttagcgcgg
     5641 tgcccatccc tgaccccacc ctgaagatga aacgcatcat ccttgaaggc agtattccct
     5701 ccccggcgaa tcctccttca ggctgtaagt tccacacacg ctgtccgcgt gccatggaca
     5761 tctgcaagca agaagttccg cccgtcatcg agctgaaact ggaccacgaa gtggcatgtc
     5821 atctctatgc tggacaggga aaggtgctgg acctataaaa tgctgaaaga atacccagac
     5881 gacgacggac gtgtcatttg cgatatgaac gttgaaggca tgcgctggta caaccggaac
     5941 ttgtcaaagg tcgacgacta tcgcaaaagc cgcgaaaaac ccgcgccata ccacagcgcg
     6001 ggcgaagtca tgacccggcg cgaaacagta cgttatacct tttatgcggt actggcagga
     6061 ctgaccatcg tggcagtctt cgccaccgcc tggattctgc tgtggctctt ctccgcgtac
     6121 gtatggttca agtaagcagt atctaatgca atagataaaa aaggctgtct tttgacagcc
     6181 ttttttctat aaaagaagca aatgtttttg tgttcgttca tttgaaaata atagaattga
     6241 atagctgagt tgatttgtgc tggattatgc ataaaaaaca caagctttta gggtgatttc
     6301 tcttgtct
//
LOCUS       DAOSFC010000051         6258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_10986_length_6258_cov_4.677253, whole genome shotgun sequence.
ACCESSION   DAOSFC010000051 DAOSFC010000000
VERSION     DAOSFC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10986_length_6258_cov_4.677253"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PK791_02270"
     CDS             <1..722
                     /locus_tag="PK791_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOH92170.1"
                     /translation="VFFIYFFEEYAWLRNLPNIWKILRWAGIGTIFIASLLLSFQNQI
                     RRLLAYFYLWETGYALLAIGLAQDGGLHYLPLIFMPRILGYAGLSYIIVKLEHLEPDF
                     DGNINNLKGFFHRYPFLSLGLISTLLSLSGLPILASFPAKRHLINLLPWSGYGMETWL
                     ILGFVGALIMTFRIIYALVSPSEEEQAIAMLAPQKEDIGIAVFCTALMLLFLISGFFP
                     SLFSGLFKGILAPFENIYLFN"
     gene            792..1982
                     /locus_tag="PK791_02275"
     CDS             792..1982
                     /locus_tag="PK791_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92171.1"
                     /translation="MDEYAGIEQLERRVEWLDNERRNDKTNLASMQNRLSVLETENAN
                     LRKQIKEIEMLVSQQSTQISSFESYNNQISRLNNEIAKQVRESSERANFNLDEASKRQ
                     RLEIDSVKTSLAKIHENLEQLSALREELKLLKVEDTRLARLIEEQKAKIVEVSRFDED
                     YRRSLQMIDEGRRQEAKRLTEAQGEITSMRKRVDETRNRIEGLADNFRSFDTRITELL
                     SFERDRKEAQSAFIDTVNSSLVEKERSFKNWEGRFQELDKINLTLNSQIEALEEARLS
                     VSKSIAQADKMTQQFERRINEISEVQRLNDERFRQEWAGFKSDDIKRWANYMLTYEEQ
                     NRESAQEIGELTRQIEEIKDISVRNQDAIDALNRETVRHVQAILRAYQDSIQNIATLL
                     DNKA"
     gene            1986..3881
                     /gene="selB"
                     /locus_tag="PK791_02280"
     CDS             1986..3881
                     /gene="selB"
                     /locus_tag="PK791_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOH92172.1"
                     /translation="MRIIGTAGHVDHGKSTLVTALTGVDPDRLKEEKAREMTIDLGFA
                     SLTLPNGESMGIIDVPGHRDFIGNMLSGIGGIDAVLLIIAADEGVSAQTREHLAILDL
                     LDIQKGFVVLTKIDLVSEPEWIDLVELEAQEVLANTSLKDAKILRVSAITGEGIPELL
                     QALQELLQDLPPKADLNRPRLPVDRVFSLTGFGTIVTGTLLDGSFAIGDEIVCMPDGK
                     PGRIRGLQNHKHKLQKIAPGHRTAININGLDKEDIKRGDVITKPGTYTPTTRLDAHFR
                     LIHDASKPLKHNTEVKVFIGSAETSARLRLLGTEKLKQGESAFIQLKLDNPIIAARGD
                     HYVLRLPSPAETIGGGVIIDPHPSRFYRRFDEDNLTRLQELHAGNESDLLVQALRSLG
                     IATGSEIVQKSGLSENVVRNLLQNLVEEDRVFVLRASPTLNKTRLTLKENWQDLQYKA
                     IEYITDFHQRQPLKAGITRENLRASLKLHQDNFDLIIEHLARQGDIKLMGHLIKHHDH
                     SVVFTNEQEKQIAPLMAKFKSQPYAPPDRAEALSYIDESLLDGLIGSGRLVAVSENIL
                     LTPDTLKEMQDWVAATIKTNGSLTMAEFRDHYQNSRKYAQAILEYMDSLGFTIRKGDI
                     RTLRIVQ"
     gene            3912..4850
                     /locus_tag="PK791_02285"
     CDS             3912..4850
                     /locus_tag="PK791_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOH92173.1"
                     /translation="MTYKVLITDGLEQIGKDIINQAGEAIDKKGIEADQLLAEAHEYH
                     GMIVRGRTKVTRQVFEAAKNLKVVGRAGVGVDNIDLEAAKEHGVTVVNCPVATTVAVA
                     ELAFGLMLAMIRRIPYADAGMKNEKWLKKELVGSELMGKTLGIIGYGNIGRRLAKYAL
                     AFGMKVICYNRRRDLDEVRSTGAEPVELEDIYTQSDIISLHLPLNPASKYMINDETFA
                     KMKDGVMIVDVARGGVMEEAALLRALDSGKVAAAALDVYEKEPPVDWSVPKHPNIIAV
                     PHIGGQTQEAQERAAEDIASEVMNVLQGKDLRWKIC"
     gene            4926..>6258
                     /locus_tag="PK791_02290"
     CDS             4926..>6258
                     /locus_tag="PK791_02290"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOH92174.1"
                     /translation="MTFEEKLKTFRNHYATTQLLGGIQGLLGWDQMTYMPMGAMQSRG
                     EQLQALGGILYDRATSPEYGQLIADLVAEIPDLDADEPVARELKLAKRSYEQSTKVPK
                     DKMMELIMTTSVAQGVWQKAKEENDFAAFQPHLQKILELNLELVEYFKPYDHPYDALA
                     DQFEPGLKTARVKEIFGALRPRQVELIQAISERPQPETDFLFQRYPIDLQRKVNQYFA
                     TKQGFDWNHGRLDESAHPFTTNFGMDDVRLTTNYDEFNAMDALFSTMHETGHGLYELG
                     ISKAYKGTTIGGTSSMALHESQSRLWENLVGRSKAFWSHFYPTLQAFFSENLANVSLD
                     KFYKAINKVSPSLIRTEADEATYNLHIMLRFEIELGMIEGRIATKDLPEIWNEKMRDY
                     LGVVPSNDTTGVLQDVHWSGGMIGYFPSYAIGNLIASQWWHQLLKDQPNTID"
BASE COUNT         1809 a         1690 c         1350 g         1409 t
ORIGIN      
        1 cggtcttttt catctacttt tttgaagaat atgcctggct tcgcaatctg ccaaatatat
       61 ggaaaatatt gcgttgggcg ggtatcggaa ccatttttat cgccagcctt ttgttgagtt
      121 ttcaaaatca aatccgacgt ctcttagcct acttttatct gtgggaaaca ggctacgcgc
      181 ttttggcaat cggactggca caagatggcg gcttgcacta tttgcccctt attttcatgc
      241 ccagaatttt gggttatgca ggattaagct acattatcgt caaactcgaa catctggaac
      301 ctgattttga tggaaatatc aacaatctca aaggcttttt ccatcgctat cctttcctta
      361 gcctgggatt aattagcact ttgttgagtt tgtctggctt acccatactc gccagtttcc
      421 ctgccaaacg ccatcttatc aatcttttac cctggtcagg ttacggcatg gagacctggc
      481 ttattctcgg gtttgttggc gcgcttatta tgacttttag gatcatttat gccttggtta
      541 gtccatcaga agaagaacaa gccatagcca tgcttgcacc acaaaaagaa gatatcggta
      601 tcgctgtttt ttgcacggct ttaatgttgc tgttccttat atccggtttc tttccttcat
      661 tatttagcgg actcttcaag ggcatactgg ccccattcga gaacatttat ctgtttaact
      721 aaactttttt tcattattga tacattctta gtataatcac actatacttt tttagataaa
      781 ggcaaaatat tatggacgaa tatgcaggca tagaacaact cgaacgacgt gtggaatggc
      841 tggacaacga acgtcgcaat gacaaaacca atcttgcttc catgcaaaac cgtcttagcg
      901 tccttgagac tgaaaatgcc aatttacgca aacaaattaa agaaattgag atgcttgtga
      961 gtcaacaaag cacacaaatt tcatcttttg aaagttataa caaccaaatt tcacgcttga
     1021 acaatgaaat cgcaaaacaa gtacgcgaaa gcagcgagcg cgctaacttt aaccttgatg
     1081 aagccagcaa acgccaaaga cttgaaatcg attctgtaaa aaccagcctg gcaaaaattc
     1141 acgagaatct tgaacaatta tcagcattac gagaagaact aaagttactt aaagtggaag
     1201 atacacgtct cgcgcgactt attgaagaac aaaaagcaaa aattgtggaa gtcagccgct
     1261 ttgacgaaga ctaccgtcgc tccctgcaaa tgattgacga aggccgtcgc caggaagcca
     1321 aacgccttac tgaagctcag ggtgaaatca cctctatgcg caaacgcgtg gatgaaaccc
     1381 gcaaccgcat tgaaggactg gctgacaatt tcaggtcttt tgacactcgc atcaccgaac
     1441 tattgagttt tgaaagagac cgcaaggaag cacaatccgc tttcattgac accgtcaaca
     1501 gcagcctggt agaaaaagaa cgcagtttca aaaactggga aggacgtttt caggaacttg
     1561 ataaaatcaa tttgactctc aacagccaaa ttgaagccct ggaagaagcc cgcctttccg
     1621 tcagcaaatc aattgcccag gcggataaaa tgactcaaca atttgaacgg cgcatcaacg
     1681 aaatcagtga agtgcagcgt cttaatgacg aacgcttccg ccaggaatgg gcgggtttca
     1741 aatcggacga tatcaaacgc tgggcaaact atatgctcac ctatgaagaa caaaatcgag
     1801 aaagcgcgca ggaaattggc gaactgaccc gccaaattga agaaattaaa gatatctccg
     1861 tgcgcaacca ggatgcaatt gacgccctga accgtgaaac cgtgcgccac gtgcaagcca
     1921 ttttacgcgc ttatcaggac tccattcaaa atatcgccac attgttggat aacaaggcat
     1981 aaactatgcg cattatcggc accgcaggtc acgtagatca tggcaagtca acccttgtca
     2041 ctgcccttac cggtgttgac cctgatcgct taaaagaaga aaaagcccgc gagatgacca
     2101 tcgacctggg ctttgcttct ttaactttgc ctaacggcga atcaatgggc attatcgacg
     2161 tccctggtca tcgcgatttt atcggcaata tgctaagtgg aatcggtggt attgatgccg
     2221 ttctgcttat tatcgccgct gatgaaggcg tatcagcgca aacccgcgaa catcttgcca
     2281 tccttgacct gctggatatt caaaaaggct ttgtcgtcct gaccaaaatc gacctggtct
     2341 ccgaaccgga atggatagac cttgtagaac tggaagccca ggaagttctc gcaaatacca
     2401 gcttgaaaga tgccaaaata ctgcgtgttt cagccattac cggcgaaggc ataccggaac
     2461 ttcttcaggc cttacaggaa ctcttgcaag acttgccccc caaggcagac ctcaaccgcc
     2521 cgcgtttgcc agttgaccgt gtctttagcc tcaccggttt tggcaccatc gtcaccggca
     2581 cacttttgga cggcagcttt gccatcggcg atgaaattgt ctgtatgccc gatggaaagc
     2641 ccggacgcat acgtggcctg caaaaccaca aacacaagct ccaaaaaatc gctcccggcc
     2701 accgcaccgc cataaacatc aacggactgg acaaagaaga catcaagcgt ggcgatgtca
     2761 tcaccaaacc aggaacatac acccccacca cacgcctgga cgcgcatttc cggcttattc
     2821 atgacgcgtc taagccactc aaacacaaca ccgaagtaaa agtcttcatc ggtagcgctg
     2881 aaacatccgc ccggttgcgc cttttgggca ccgaaaaact caaacaaggt gaaagcgcct
     2941 tcattcaact caaactcgac aatcccatca tcgccgcccg tggtgaccat tacgttttac
     3001 gtctgccctc gccggctgaa accatcggtg gtggtgtcat cattgacccg cacccaagcc
     3061 gtttctaccg gcgctttgac gaagataacc tgacccgcct tcaggaactc cacgccggca
     3121 atgagagtga tttgcttgtg caagccttgc gcagtttagg catcgcgacg ggcagcgaaa
     3181 ttgttcaaaa atcaggcctt tcggaaaatg tcgtcagaaa tcttttacaa aacttagtcg
     3241 aagaagaccg cgtattcgtc ctgagggcca gtcccacgct caacaaaacc cgcctaaccc
     3301 taaaagaaaa ctggcaagac ttgcaataca aagccattga atacatcacc gattttcatc
     3361 aacgccagcc cctgaaagcc ggcatcacac gtgagaattt gcgcgccagt ctaaaactcc
     3421 atcaggacaa ttttgacctt atcatcgaac atctcgccag gcaaggcgac attaaactca
     3481 tgggacacct catcaaacat cacgatcata gcgtggtatt cactaatgaa caggaaaagc
     3541 aaatcgcgcc cctcatggca aaattcaaaa gccagcccta tgccccacca gaccgcgctg
     3601 aagccctaag ctacattgat gaaagcctgc tggatggtct cattggcagt ggacgcctgg
     3661 tggcagtatc agaaaacatt ttactcacac cagatactct aaaagaaatg caagactggg
     3721 ttgctgccac aatcaagacg aatggcagtt tgaccatggc agagttccgc gatcattacc
     3781 aaaacagccg aaaatacgcc caggccatcc ttgaatatat ggatagtttg ggatttacaa
     3841 tccgaaaagg tgacataaga accctaagga tagtacaata gagatagcaa taataaacat
     3901 aaggagaacc tatgacatac aaagttttga ttaccgatgg ccttgagcag attggcaagg
     3961 acatcatcaa ccaagcaggc gaagcgattg acaagaaagg cattgaagcc gatcaactgc
     4021 tcgccgaagc ccacgaatac cacggcatga ttgttcgcgg acgcaccaag gtcacccgcc
     4081 aggttttcga agctgcaaag aatctcaaag ttgtcggccg cgctggtgtt ggtgttgata
     4141 acatcgacct cgaagctgcc aaagaacacg gcgtcaccgt cgtcaactgc cccgtcgcca
     4201 ccaccgtggc tgtagccgaa ttagcctttg gcttgatgct cgccatgatt cgccgtattc
     4261 catacgccga cgctggcatg aaaaacgaaa aatggctcaa gaaagaactt gtcggctccg
     4321 aactcatggg caaaaccctt ggcatcatcg gttatggcaa catcggccgt cgcctggcca
     4381 aatacgccct ggcatttggc atgaaagtca tctgctacaa ccgccgcaga gatctcgacg
     4441 aagtccgctc aaccggtgca gaacccgttg aacttgaaga catctatacc caaagcgaca
     4501 tcatcagcct ccatctccct ctcaaccccg cttccaaata catgattaac gacgaaacct
     4561 tcgccaagat gaaagacggc gtgatgatcg ttgacgttgc ccgtggcggt gtgatggaag
     4621 aagccgctct cctgcgcgcg cttgattccg gcaaagtggc tgccgctgct ttggacgttt
     4681 acgaaaaaga accacccgtt gactggtccg tgcccaagca ccccaacatc atcgcggtgc
     4741 cccacatcgg cggacaaacc caggaagctc aggaacgcgc cgcagaagac atcgcctccg
     4801 aagtgatgaa cgttctgcaa ggcaaagacc tgcgctggaa gatttgctaa ataagctgta
     4861 aacccgataa cattcgccta aaaagcgaat gttatccttt aaggacactt ttataaggaa
     4921 aagccatgac ttttgaagaa aaactcaaaa cctttcgcaa ccattacgct accacacaac
     4981 tgttaggtgg cattcaaggt ctgttgggat gggaccaaat gacctatatg cctatgggcg
     5041 ccatgcaaag ccgtggcgaa cagcttcagg ccttgggtgg catcctttac gaccgcgcca
     5101 ccagtcccga atatggccaa ctcatcgctg atctggtggc tgaaatccca gaccttgacg
     5161 cggatgaacc cgtcgcccgc gaactcaaac tcgccaaacg cagttatgaa caatccacca
     5221 aagtccccaa agacaaaatg atggaactca tcatgaccac cagcgtggca caaggtgttt
     5281 ggcaaaaagc caaagaagaa aacgatttcg ccgcttttca gccccacttg caaaagattc
     5341 tcgaactcaa cctggaactg gtggaatact tcaaacccta cgaccatccc tacgatgccc
     5401 tggcagatca atttgaacca ggtttgaaaa ccgcccgcgt taaagagatt tttggcgctt
     5461 tgcgaccccg tcaggttgaa ctcatccaag ccatcagcga acgcccccaa cccgaaaccg
     5521 acttcctttt ccagcgttat cccatcgacc tgcaacgcaa agtaaaccag tatttcgcca
     5581 ccaaacaagg ctttgactgg aaccacggca gactggacga atccgcccac cccttcacca
     5641 ccaatttcgg catggacgac gtccgtctca ccaccaatta cgacgagttc aacgccatgg
     5701 acgccctctt tagcaccatg cacgaaacag gacacggcct ttacgaactg ggcatcagca
     5761 aagcctataa aggcactacc atcggtggca catcctctat ggcactgcac gaatcgcaat
     5821 cgcgcctttg ggaaaacctc gttggacgtt ccaaagcctt ttggtcacac ttttacccga
     5881 ctctccaggc cttctttagc gaaaatctcg ccaatgtcag cctggacaaa ttttacaaag
     5941 ccatcaacaa agtcagccca tcgcttattc gcactgaagc tgatgaagcc acctataacc
     6001 tgcacatcat gttgcgcttt gaaattgaac tgggcatgat tgaaggtcgc atagctacaa
     6061 aagacctgcc cgaaatctgg aatgagaaaa tgcgcgacta cctgggcgtc gttcccagca
     6121 acgacacaac cggcgtcctg caagacgtgc actggtcagg tggtatgatc ggctacttcc
     6181 ccagctacgc gataggcaac ctgattgcct cacaatggtg gcatcaattg ctcaaagacc
     6241 aacccaacac gattgacg
//
LOCUS       DAOSFC010000052         6216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_11092_length_6216_cov_4.837851, whole genome shotgun sequence.
ACCESSION   DAOSFC010000052 DAOSFC010000000
VERSION     DAOSFC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11092_length_6216_cov_4.837851"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK791_02295"
     CDS             complement(<1..480)
                     /locus_tag="PK791_02295"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOH92175.1"
                     /translation="MTDMNTQNSPDPSDWQGLPEQESPINKPEMPSQTPPNGIPEQPT
                     SAPAEQQTVFITPQTVSPILSYLIMGLTILFFIGQQLSSYLFDADLILVFFAKVNPAI
                     VSGELWRLVTPVLVHGGIIHIGLNMYALYILGRDVESLFGYGRFVLLYSLSAFGGNVL
                     "
     gene            complement(477..1103)
                     /gene="gmk"
                     /locus_tag="PK791_02300"
     CDS             complement(477..1103)
                     /gene="gmk"
                     /locus_tag="PK791_02300"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOH92176.1"
                     /translation="MQKSSLVFGAPNQYPLLIVISGPSGIGKDAVVGLLRKNTPDTHF
                     VITMTSRAPRVDETHGVDYFFVDRETFEDHIAKDELLEYAKVYSDYKGIPKSQVREAF
                     ATGKDVIMRLDVQGAMTIRELCPDAILIFLTASSKEEWMQRLIARRSETPDELALRIE
                     TAEKEYALIEEFDYLVVNSHMNIEQTISDIQAIIRTEHMRTKPRKVEL"
     gene            complement(1145..2323)
                     /locus_tag="PK791_02305"
     CDS             complement(1145..2323)
                     /locus_tag="PK791_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92177.1"
                     /translation="MPLEPLDGVRSLADGKRFDYVVWTLKALFNKAVSASLGVEKFLN
                     DEQQATIVKAYFDQVGVVEEKERALEDVLYHSSFDESQFQLANEDLNEARTWLKKLAP
                     LAEAIIQNQTEHILLDMGFGYGGQIIPPVLYEVTDLPLNLIVSARDEIGTVISISLKP
                     GIDEIEKNEIENDALNRYDYSALIEEVGGVGTYPTMVLRSNSINWLFETVAHEWIHNY
                     LTLRPLGARYFTNNAMRTINETTASLAGKEIGRAVIKIFYPEHIVIGTYPKRELNWVI
                     SEEEQNAKGFNYRAEMRETRVTVDELLAQGQILMAERYMEQRRLVFWDEGFRIRKLNQ
                     AYFAFYGSYNDTPGGGASGKDPVGPAVRALRYSLPHLKAFIDTIQDVRSLDDLMKIAP
                     "
     gene            2536..>3377
                     /locus_tag="PK791_02310"
     CDS             2536..>3377
                     /locus_tag="PK791_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92178.1"
                     /translation="MEKTNARNVILIIIIIFLFFLLACCIVSIFGFSGVIKETISYML
                     TQTPIITPALPIKQTPTSQPSIPIPTSADHDEAAQETWQTLSGIIIPEADSIDLAQRL
                     AGKQNVPEVFIDTNAPYSLGAKKAFWIINSDTTESSSIDATLHYLGERIYIWIDDRVS
                     FRDSSLQTLGDIIDQDIVPTNREFFGMEWNPGVDGDERFYILYTKGIGKWVAGYFSSA
                     DEVHPDVHPYSNAHEMFFLSADNVRLEDKYILGTIAHEYQHMIHWYLDKNEETWVNEG
                     FSMLA"
     assembly_gap    3378..3477
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3478..4311
                     /locus_tag="PK791_02315"
     CDS             <3478..4311
                     /locus_tag="PK791_02315"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOH92179.1"
                     /translation="YSPYKLSITESIYSCPEYIQRDVHQFGVDYNEIKCDGVKNIAFQ
                     GNRYVKVLPIDEIPSGDYFWWSNAADESNMSLQREFDLSDVTGQATFSFWQWYDIEDD
                     YDYAYLTASTDGESWEMLKASNCTEYNPSGNNFGCGWTGSSDGWAEIEVDLSAYAGQK
                     VTLRFDYVTDAAVNSHGMVLDDFKLEAIGYTDDVESGDGGWTSQGFARIQNILPQSYA
                     ISVVRYSNEIQVEHYFVSGDTPLQIELSKSPAREKIIIIISGTTLYTRQKTDYQLLFG
                     E"
     gene            complement(4437..4769)
                     /locus_tag="PK791_02320"
     CDS             complement(4437..4769)
                     /locus_tag="PK791_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOH92180.1"
                     /translation="MEFVITEYKRCFVIKADGRIDSTSAQEVEKRLMELTNSEKSIII
                     DMQAVNFVSSAGWWAIIRVQKELKNKHKADLVLVNLQENVRDSMDLIGILPYFTVYEE
                     MIDAIASI"
     gene            4944..>6216
                     /gene="miaB"
                     /locus_tag="PK791_02325"
     CDS             4944..>6216
                     /gene="miaB"
                     /locus_tag="PK791_02325"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOH92181.1"
                     /translation="MNVADSERLSRALEKLGYQSTNLADEADIIVLNTCVVRQSAEDK
                     ALGRLNSLKPLKSAKPHLIIAVMGCLVGNKGNPALEKRFPWVDAFSAPSDPESILRTV
                     RERHASLFEPISKTGEDDITSLLQEEFGIYTSNQHAVSENLPIVLGCSHACAYCIIPS
                     KRGREISRDPKRIIEEAKTMVARGAKEIVLLGQIVDRYGLDRDDGIHLAELLAMVNDI
                     DGLERIRFLTSHPNYFTDDMIKAVATLPKVMPHIELPIQAGDDEVLKAMRRGYTNAQY
                     RDIIAHIRDLMPDVSIGTDIIVGFPGESEAAFEETYKLLADLKLDVAHLARYSPRPGT
                     YSERFLADDVSAEEKMRRFRKLESLQKQVVGEINQRFLGQSVPVLFEERTKGKWRGRS
                     PHNRLVFVESDENLLGQIRDVHIDWAGPWSLL"
BASE COUNT         1688 a         1511 c         1283 g         1634 t
ORIGIN      
        1 gagaacattg ccaccaaacg cactcagcga atagagaaga acaaagcggc catatccaaa
       61 caaagattca acatcacgtc ctaaaatata aagcgcgtac atgttcagac cgatatggat
      121 aatgccccca tgcaccagca caggtgttac caggcgccac aattcgccag aaacaatggc
      181 agggtttacc ttggcaaaaa acaccaggat aagatcagcg tcaaacaggt aggaagataa
      241 ttgctggccg ataaaaaaca gtatggtaag acccataata agatagctaa gaatcgggct
      301 aaccgtttgt ggcgtgataa aaacagtttg ctgttccgcg ggcgcgctgg tcggctgttc
      361 tggaatgcca tttggcggtg tttgtgaagg catttcaggc ttatttatag gactttcctg
      421 ttcaggcaag ccttgccagt ccgatgggtc aggactgttt tgtgtattca tatctgtcat
      481 aattcaacct tgcggggttt cgtgcgcatg tgttccgtgc ggataatcgc ctggatatca
      541 ctaatcgtct gttcgatatt catatgtgaa ttgacgacca aataatcaaa ctcttcaatt
      601 aaggcatatt ctttttctgc ggtttcaatg cgcagtgcca gttcatctgg tgtttcgctg
      661 cgtcgtgcga ttaaacgttg catccattct tccttacttg acgcggtcag gaaaatgagt
      721 atagcatcag ggcaaagctc acgaatggtc atcgcgcctt gcacgtcaag ccgcataata
      781 acatccttgc ccgtcgcaaa agcttcgcgc acctgagact tgggaatccc cttatagtca
      841 gaataaacct tggcatattc caaaagttcg tctttggcaa tatggtcttc aaacgtttcc
      901 cgatccacaa agaaatagtc cacgccatga gtttcgtcca cccgtggcgc gcgcgaggtc
      961 atcgtaatca caaaatgcgt atcgggggtg ttttttcgca acaaaccaac caccgcgtct
     1021 tttccaatac cacttggacc agagataaca attaatagcg ggtattgatt tggggcaccg
     1081 aagacaagtg aacttttctg catagcttaa tctcttttca attattctcg ttcattataa
     1141 cgaactatgg cgcgattttc atcaggtcat ccaaacttcg cacgtcttga attgtatcaa
     1201 taaacgcctt taggtgtggc aggctgtaac gtaaagcgcg caccgcaggt ccgactggat
     1261 ctttgcccga agcgccacct cctggcgtgt cattatagga accataaaac gcgaaataag
     1321 cctgattaag tttgcgtata cgaaagccct catcccaaaa gaccagtcgg cgttgttcca
     1381 tataccgttc cgccataaga atttgtcctt gcgcaagcaa ttcatccacc gtcacgcgtg
     1441 tttcgcgcat ctctgcccga taattgaaac ctttagcatt ttgctcttct tcgcttataa
     1501 cccaattaag ttctcgtttt gggtatgtcc ctatgacaat atgttctgga tagaaaattt
     1561 tgattaccgc ccgcccaatt tctttcccag ccagacttgc cgtcgtttcg ttgatggtcc
     1621 gcatggcatt attcgtgaaa tagcgcgctc ccagcggacg taaggtgaga tagttatgaa
     1681 tccactcatg cgccacggtt tcaaacagcc agttgatgct attactgcgc aaaaccatag
     1741 tcggataagt accaactcca ccgacttctt caattagcgc agaatagtca taacgattaa
     1801 gggcgtcatt ctcgatttca ttcttttcga tttcgtctat gcctggtttt aggctgatac
     1861 ttattaccgt tccaatttca tctcgggcag atacaatcaa attcaggggc aaatcagtca
     1921 cttcatacaa cactggtgga ataatttgcc cgccatagcc aaagcccata tccaatagaa
     1981 tgtgttctgt ctggttttga atgattgctt cggcaagagg tgccagtttt tttagccagg
     2041 ttctcgcttc atttaagtct tcgttcgcca gctgaaactg gctttcgtcg aaacttgagt
     2101 gataaagcac gtcctcaagt gcgcgttcct tttcttccac cacgccaacc tggtcaaaat
     2161 aggcttttac tatagtcgcc tgttgttcat catttaaaaa cttttccacc cccaatgacg
     2221 ccgacactgc tttattaaac agcgctttca atgtccacac aacgtaatca aagcgcttgc
     2281 catccgccag acttctcaca ccatccaatg gttcaagtgg caaaacagat gacgcccgca
     2341 ggccaaaaaa gaatatcaaa atcaatatca gccacaaaaa ttgtttgcga tgtctgcatt
     2401 ttgtattttc tgtctggcat gccatgccta tattttacac tcatctggaa tacttgtttt
     2461 tacatagtct gaatttgcaa cttgcattat aagctttata attgtcacgg taaatttgcc
     2521 cagaaaagga tagttatgga aaaaacaaac gcacgcaatg tcatcttaat catcattatc
     2581 atctttttat tctttctttt ggcatgttgt attgtttcaa tcttcggttt ttccggtgtc
     2641 atcaaagaga cgataagcta tatgcttacc cagacaccta tcatcacacc agccttacct
     2701 atcaagcaaa cgccaacatc tcaaccttca ataccaatcc caacgtctgc tgaccatgac
     2761 gaagccgccc aggaaacctg gcaaacgctt agcggaatta ttatcccgga agccgacagc
     2821 attgacctgg cccagcgact cgctggcaag caaaatgtgc cggaagtttt catcgacacc
     2881 aatgcgcctt acagtctcgg cgcaaaaaaa gccttttgga ttatcaattc cgacactact
     2941 gaatcttcat ccattgacgc cacactccac tatcttggcg agcgcatcta catttggatt
     3001 gacgatcgtg ttagcttccg cgacagttcc ctgcaaacgc tgggcgacat cattgaccag
     3061 gacatcgtcc ccaccaatcg cgaattcttt ggcatggaat ggaaccctgg cgtggatggt
     3121 gacgaacgct tttacattct ttacaccaaa ggcatcggca aatgggttgc gggttacttt
     3181 tcttccgcag atgaagtcca ccccgacgtc cacccctact ccaacgcgca tgaaatgttc
     3241 tttctcagcg cggacaacgt ccgcctggaa gataaataca tccttggcac gattgcccat
     3301 gaataccaac acatgattca ctggtatctc gacaaaaatg aagaaacctg ggtaaacgaa
     3361 ggtttctcta tgcttgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntat
     3481 tcaccctata agcttagcat caccgaaagt atttacagtt gccctgaata cattcagcga
     3541 gatgtacatc agtttggcgt ggactataac gaaataaaat gtgatggcgt aaaaaatatc
     3601 gcttttcaag gcaatcgcta cgtaaaagta ttgcccattg acgaaattcc ctcaggggat
     3661 tacttttggt ggtccaatgc tgccgatgaa agcaacatga gcctgcaacg cgagtttgac
     3721 ctcagcgatg taacgggtca ggcaaccttc agtttttggc aatggtacga cattgaagat
     3781 gattacgact atgcttatct caccgcttca actgacgggg aaagctggga gatgcttaaa
     3841 gccagtaact gtaccgagta caatccttct ggtaacaact ttggctgcgg ctggacaggt
     3901 tccagcgatg gctgggcaga aattgaagtg gatttaagtg cctatgccgg tcaaaaagtg
     3961 actttgcgtt ttgactatgt taccgatgcc gcggtgaact cccacggtat ggtcttggac
     4021 gatttcaaac tcgaagccat cggttacact gatgacgtcg agtccggcga tggtggatgg
     4081 accagccagg gttttgcgcg cattcaaaac atcctgcccc aaagctatgc gatcagcgtt
     4141 gtgcgctata gcaatgaaat ccaggtcgaa cactattttg tgagtggcga cacgccgtta
     4201 caaatagagt taagcaaaag tcccgccaga gagaaaatca tcattatcat cagtggcaca
     4261 accttataca cccgacagaa aacggattat caattactgt ttggggaata agtcaatact
     4321 tgtattgcaa ttgactaatt agcattataa aaaaaggtat actgaaagca acttaatagc
     4381 aaaaaagcca gcccaaatta aaaaggctgg cttttctgat atcaggtagt gtcattttag
     4441 atgctggcta tagcatcaat catttcttcg taaacggtaa agtaaggcaa aataccaatc
     4501 aaatccatcg aatcgcgcac gttttcctgc aaattcacca ggacaaggtc ggctttatgc
     4561 ttgttcttga gttctttctg aacgcgaata atcgcccacc accctgcact ggaaacgaaa
     4621 ttaaccgctt gcatgtcgat gatgattgat ttttcagaat tagtcagctc catcaggcgt
     4681 ttttcaactt cctgtgcgga agtgctgtca atacgcccat cagctttgat aacaaaacaa
     4741 cgtttatatt cagtaattac aaattccatt atttcctcga cctcactagt tgattttatc
     4801 gacgttagca ttatatcatc tttaatatta tatccccaat aattgtcaag tattcattct
     4861 taaaaaataa aaatcaatat aaagaactgt attcaagtat aatctgggct atgaaatatc
     4921 atatttggac tataggctgc caaatgaatg tggcagattc cgagcgcctc agtcgcgcct
     4981 tggaaaaatt aggctatcaa agcacaaatc ttgctgatga agcagatatc atcgttctta
     5041 acacctgcgt cgtcaggcaa agcgctgaag acaaagccct tggcagactt aactcattga
     5101 aacctttaaa gtctgccaaa ccacacttga ttatcgccgt gatgggttgc ctggttggca
     5161 ataaaggcaa ccccgcactt gaaaaacgtt tcccatgggt agatgcattt tccgcaccct
     5221 ctgaccccga aagcattttg cgaactgtcc gggaaagaca cgcaagtcta ttcgagccaa
     5281 tctcaaagac aggcgaagac gacatcacca gtctcttaca ggaagaattt ggcatctata
     5341 ccagcaatca acatgccgtc agcgagaatc tgcccatcgt tctcggttgt tcccatgcct
     5401 gcgcttactg catcattccc agcaaacgtg gccgggaaat cagccgtgac ccgaaacgca
     5461 tcattgaaga agccaaaaca atggtcgccc gcggtgccaa agaaatcgtc ctgttgggtc
     5521 aaatcgttga ccgctacggt cttgaccgcg acgacggtat acatctggcc gagttgctgg
     5581 caatggtgaa cgatattgat ggtttggagc gcattcgttt tctgacttcg caccccaact
     5641 acttcaccga tgacatgatt aaagccgtgg caaccttgcc caaggtcatg ccccacattg
     5701 aactgcccat tcaagccgga gatgacgaag tgctcaaagc catgcgtcgt ggttacacca
     5761 atgcccaata tcgcgatatc atcgcccaca tccgcgactt gatgccggat gtttcaatcg
     5821 gcaccgacat catcgttggt tttccaggtg agtccgaagc cgcctttgag gaaacctaca
     5881 aactgcttgc tgacctgaaa ttagacgtcg cccatttggc acgctactcc ccgcgcccag
     5941 gcacctattc tgaacgtttc cttgcggatg atgtctccgc tgaagaaaaa atgcggcggt
     6001 tccgaaaact ggaaagcctg caaaagcaag ttgttggcga gattaaccag cgctttttag
     6061 gccaatccgt acccgtttta tttgaagaac gcaccaaagg taaatggcgt ggccgaagtc
     6121 cacacaatcg ccttgtcttt gttgaatcgg atgaaaactt attaggtcaa attcgcgacg
     6181 tgcacatcga ctgggcaggt ccatggtcac tcttgg
//
LOCUS       DAOSFC010000053         6147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_11246_length_6147_cov_5.391005, whole genome shotgun sequence.
ACCESSION   DAOSFC010000053 DAOSFC010000000
VERSION     DAOSFC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11246_length_6147_cov_5.391005"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2146)
                     /locus_tag="PK791_02330"
     CDS             complement(<1..2146)
                     /locus_tag="PK791_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOH92182.1"
                     /translation="MIPKRLSLSGFTSYRKTVELDFTKFNLACISGPNGAGKSSILDA
                     ITYALYGKARKSDDALINSASDKAESMLDFEYEGQTYRVNRSITRGKGSSLDFYILNP
                     QATDDSQRWKTLTERTIRETDAKIERTLRLDYETFINASFFLQGKADSFATQRPADRK
                     RILSSILGLNQWDEYLEASRARSRQVRLELDIIDAKLAEIQNELDQEEAHKTRLKELE
                     DKLKLASAESKVLQAQLDTERARHKTLDEKRQYVMLLKQNFERAQSKHEITQQRHHER
                     QNQLATYTEILNKAPETEAAYQTWQKLRDELQAFDKIYEQFKPMDGKRQDLLKQIELK
                     RQSLQQALQHLLDERKSVEETLIQSAAFEKQRLELIPKIEKLQEELAQAETLQEDIQD
                     LVALRSTLMSENNTLRDKMNELKERQIQVAKVEGAQCPFCEQELSPQHRQSLIEKLEL
                     EGKGMAEQWHDNRQKFQEADIALKDKNQALQTRGQNQSNLQALIREADRLEQLLIQNE
                     QKKKTFESDSAPKIRALESELKNEAFLPEARLEIQKIEQELATLGYNVEAHATARRNE
                     LLARDAETAYHELGKARSTVEQIKLNLAELEIELKDTAKERDKQEEAFKKAGAELAAE
                     EAGLSDLRALEHDFVAQKEAEASLLRELGGARQLLDNLDTQRARQQELRDEQETLRHK
                     QGQLRSLETAFGKDGVPAMLIEQALPELEAQAN"
     gene            complement(2149..>2687)
                     /locus_tag="PK791_02335"
     CDS             complement(2149..>2687)
                     /locus_tag="PK791_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92183.1"
                     /translation="YEKDIDYLLVTNLITRKIREAWETATAEFHSKKGNEPRPLVVVV
                     EEAHKLLNREMASQTIFSTIAREMRKYYVTLLIIDQRPSQIYDEVMSQLGTRISASLG
                     DEDDINAVLSGLPGRGGLRGMLAKLQPKEEVLMLGWGVPMPIPIRSRRYDQAFWDDLL
                     GEQNKAKSKEDALRRMGF"
     assembly_gap    2688..2697
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2698..3882)
                     /locus_tag="PK791_02340"
     CDS             complement(<2698..3882)
                     /locus_tag="PK791_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH92184.1"
                     /translation="MMTTQNPNNLSPNNEPIGFISGGGLEANLQVRLTVPAQTVQSGA
                     FVVMDSGYWRFYGLVTDLQLGATDLQYANQLNINRFDPALATILNKQTLFTNLAVMPA
                     LMQEIGPDPGTPEYGQWQSSLTEKPRPMPVKTVPEHHTPVRLASKGDIEDIFGKESKT
                     VFRVGETREQGHAVCLDLEKFVQRSSGIFGTTGSGKSFLARMILAGLIHADKASTLIF
                     DMHNEYGIDSTSSDTGAAVPGLKGKFSSKVRLVGLGADSTFHGRQPDFTLEIAHSDIT
                     PEDVLLLAKTLNLTDASGILLDTLQSKFKEKWFTTFMDMKSGDGALYTDEDGKQRPTE
                     DSVAAWANEVGAHVGSAEALHRKLKALVNRKYLVEEPASNALDQIINNLQEGRHVVLS
                     FGN"
     gene            complement(3888..4496)
                     /locus_tag="PK791_02345"
     CDS             complement(3888..4496)
                     /locus_tag="PK791_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92185.1"
                     /translation="MNEIQTIPIGKLLRADNRQFVAGCRVKELETPALGALVKVRLQD
                     QSEVYGLITRISISDDGLVRQLASGMSIPPEYTADNRHNRNLPVEIEVLTLGFAQDGK
                     LYHQLPPRPPLSLDVLYLCDDAELVRFTGHGKHAYLRHILRLQDMPVEEILVSHLSKA
                     RHAHEAAFNHDWYLKACEQLVILLREDYPRLTTVLDALSAME"
     gene            complement(4499..5674)
                     /locus_tag="PK791_02350"
     CDS             complement(4499..5674)
                     /locus_tag="PK791_02350"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOH92186.1"
                     /translation="MPINYAHIESNLDRFADLTRKKLDEMRQNYQNATAQIEIAAREQ
                     DAVRQAVERVERQAKSLYTAKPSDEAMLASFPLPACPEKYTLIAADGSQINPSRHRAL
                     QFCLINIGLIHVEHGSGHAPTQNVYTKLLDQDDLYTNEGFLIGEEEVALHRDLLERQF
                     LLEATHLEPSMPTITLTDGPLEVFTGASRSGERRQRVQKQNAVINEHMQRRGIINAGY
                     IDKPGAEMINRMLDLYVQTKNDPSQSYDDKKRANKGVSDLALFEKRLAPGERSAIFEA
                     VSKNENAEGRQEVCFFLLNVSNDPERAWLARVEFPKWVAEDPEKVNLLHAVLYRDAQV
                     LDKTPYPYVLHRAHELAVVVIAEHDEVEEMLLSCLDHEHLPSGTMSHKQSLKSLPKR"
     gene            5856..>6147
                     /locus_tag="PK791_02355"
     CDS             5856..>6147
                     /locus_tag="PK791_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOH92187.1"
                     /translation="MKSNKWILIIGGILAVGLMLTACTHPQVTKEVESIRTLSVSGSG
                     QVTLVPDIARVNIGVHSEAELVTVAVNENNKQAQAITDALLKKGIEAKDIQTA"
BASE COUNT         1298 a         1467 c         1657 g         1715 t
ORIGIN      
        1 cgttggcttg cgcttcaagt tcgggcaggg cttgttcaat gagcatggcg ggcacgccgt
       61 ccttgccaaa agcggtttcg agactgcgca actgaccctg tttgtggcgg agcgtttctt
      121 gctcgtcgcg cagttcctgc tggcgggcgc gttgcgtgtc gagattgtcc aaaagttgac
      181 gggcaccgcc caactcgcgt agcaggctgg cttcggcttc tttttgagcc acaaagtcgt
      241 gttcgagagc gcgcaaatcg gataagcccg cttcttccgc agccagttca gcgccggctt
      301 ttttgaaggc ttcttcctgc ttatcgcgct cttttgcggt gtctttcagt tcaatctcaa
      361 gttctgccag gtttagtttg atttgttcca ccgtgctgcg cgccttgccc agttcatgat
      421 aggcggtttc agcatcgcgg gctaagagtt cattgcgtcg ggcggtggca tgggcttcaa
      481 cgttataacc cagcgtggca agttcctgct cgattttctg gatttccagg cgcgcttcgg
      541 gcaggaaggc ttcattttta agttcgcttt cgagcgcgcg aatttttggt gcagaatcgc
      601 tttcaaaggt cttctttttc tgctcgtttt gaataagcag ctgttccaac cggtctgctt
      661 cgcggataag ggcttgcaag ttgctttggt tttgaccgcg cgtttgcaag gcttgatttt
      721 tgtctttcaa ggcgatgtcg gcttcctgaa acttttgcct gttgtcgtgc cactgttctg
      781 ccatgccttt gccttcaagt tcaagttttt cgattaaact ttggcgatgt tgtgggctta
      841 actcctgttc acagaaagga cattgcgcgc cttccacttt ggcgacctga atctgacgct
      901 ctttgagttc gttcatctta tcgcgcaagg tgttgttttc gctcatcagc gttgatctta
      961 gggcgaccag gtcttgaatg tcttcctgca aagtttctgc ctgggcgagt tcttcctgta
     1021 gtttttcaat tttggggatt agttcaaggc gttgcttttc gaaggcagca ctttgtataa
     1081 gggtttcttc gacacttttg cgttcgtcaa gcaaatgctg cagggcttgt tgcaaggatt
     1141 gacgtttaag ctcgatttgc ttgagtaagt cctggcgttt gccatccatc ggcttgaact
     1201 gctcgtaaat cttatcaaag gcttgcagtt catcacgtaa tttttgccag gtttgataag
     1261 cggcttcagt ttcgggcgct ttattgagaa tctccgtgta agtcgccagt tggttttggc
     1321 gttcgtggtg ccgttgctgg gtaatctcat gcttgctttg ggcgcgctcg aagttttgct
     1381 ttaagagcat aacgtactgg cgcttttcgt ccagtgtttt gtggcgggcg cgttcggtgt
     1441 caagctgcgc ctggagcact tttgattctg ctgaagcgag tttgagtttg tcttccagtt
     1501 ctttcagacg cgttttatgc gcttcttcct ggtcgagttc gttttgaatt tcagctaact
     1561 tagcgtcgat gatatccagt tcaaggcgca cctggcggga acgcgcgcga ctggcctcta
     1621 ggtattcgtc ccactgattg agccccagga tgcttgaaag aatgcgcttg cggtccgcgg
     1681 gtctttgggt cgcaaatgaa tccgctttgc cttgcagaaa aaacgacgcg ttgatgaagg
     1741 tttcgtaatc caggcgcaaa gtgcgctcga ttttagcgtc cgtttcgcga attgtgcgtt
     1801 cggtgagtgt tttccagcgc tggctgtcat ctgtggcctg ggggttgaga atataaaagt
     1861 caagactgct gcccttgccc cgcgtaatgg agcggttaac gcgatatgtt tgcccttcat
     1921 actcaaaatc aagcatagat tctgctttgt cactcgccga attaatcagc gcgtcatctg
     1981 atttgcgcgc cttcccataa agcgcgtagg tgatggcgtc taaaatggac gattttcccg
     2041 ccccgttggg tccggaaata caagccaggt tgaattttgt aaaatccagt tcaaccgttt
     2101 tgcggtaaga agtaaagccg gaaagggaaa gtcgttttgg aatcattctt agaaacccat
     2161 ccttcgcaga gcgtcttctt tgctcttagc tttgttttgc tcacccagta aatcgtccca
     2221 aaaagcctgg tcatagcgac gtgagcgaat gggaataggc atgggcacac cccatcctaa
     2281 catgagcact tcttctttgg gttgcaactt tgccagcatt ccgcgcaatc caccgcgacc
     2341 gggcaaaccg gaaagtaccg cgttgatgtc atcttcatca ccgagcgacg cggaaatccg
     2401 cgtgccgagt tgcgacatca cctcgtcgta aatttgcgac gggcgctgat cgatgattaa
     2461 tagggtaaca taatacttac gcatttcacg ggcgatggtg ctgaaaatgg tttggctggc
     2521 catttcgcga ttgagcaatt tatgcgcttc ttcaaccacg acaaccaagg ggcgtggttc
     2581 gttgcctttc ttgctgtgaa attccgcggt ggctgtttcc caggcctcgc gaattttacg
     2641 ggtaatcagg ttggtgacca aaagatagtc gatgtctttt tcataatnnn nnnnnnnatt
     2701 gccaaaggaa agcactacat ggcgaccttc ctgcaggtta ttgatgattt gatcaagcgc
     2761 gtttgatgcg ggttcttcaa cgagatattt gcggttgacc agcgctttta gcttgcgatg
     2821 taaagcttca gccgagccca cgtgcgcgcc gacctcattt gcccaggcgg caacgctgtc
     2881 ttcggtgggg cgttgtttgc cgtcttcgtc agtatagagc gcgccgtcgc cggatttcat
     2941 gtccatgaag gtagtaaacc atttttcttt aaacttgctt tgaagggtgt ccagcaaaat
     3001 accactggcg tcggtcaggt tcagggtttt ggcaagcaat aaaacgtctt caggcgtgat
     3061 atcgctgtgg gcgatttcca aagtgaaatc aggctgtcgg ccgtggaaag tgctgtccgc
     3121 gcctaaaccg accaggcgta ctttgctgga gaacttccct ttcaggccgg gcaccgcggc
     3181 gccggtatct gaactggtag agtcgatgcc gtattcattg tgcatgtcaa agatcaacgt
     3241 ggaagcctta tcagcgtgaa ttaatcctgc taaaatcatg cgtgccagga aggactttcc
     3301 ggagcccgtt gtgccaaaaa tgcccgaact gcgctgaaca aacttttcca ggtcaaggca
     3361 aaccgcatgt ccttgttcgc gcgtttcgcc aacacgaaag accgttttgc tttccttgcc
     3421 aaaaatgtct tcaatatccc ctttacttgc cagacgtact ggggtgtggt gttctggcac
     3481 ggttttaacc ggcataggtc ttggtttttc ggtaagtgag ctttgccatt gaccgtattc
     3541 gggtgtgcca gggtcaggcc cgatctcttg catcagggca ggcatgactg ccaggttggt
     3601 aaagagcgtt tgcttgttta aaatggtggc gagagcaggg tcaaaacgat taatgttgag
     3661 ttggttggcg tattgcagat ctgttgcgcc gagttgtagg tcggttacca agccataaaa
     3721 acgccagtag ccgctgtcca tcactacaaa ggcgccactt tgcacggttt gcgccggcac
     3781 ggtaagacgc acttgcagat tggcttccag tccaccgcca gaaatgaatc ctatcggctc
     3841 attattcgga cttagattgt tagggttttg tgttgtcatc attcacctca ttccattgcc
     3901 gaaagggcgt ccagcacggt ggttaatcgc gggtagtcct cgcgcaacaa aattaccagt
     3961 tgctcacagg ctttcaaata ccagtcatga ttgaaggctg cctcatgcgc atggcgcgct
     4021 ttggacagat ggctgaccag aatctcttca acaggcatgt cttgcaagcg caggatatgg
     4081 cgcagataag catgcttgcc atgcccggta aagcggacga gttcggcgtc atcgcataaa
     4141 taaagcacgt caaggctcaa aggcgggcgc ggggggagct ggtggtacag tttgccatct
     4201 tgcgcgaaac caagcgtgag cacttcaatc tcgactggca ggttgcggtt gtggcggtta
     4261 tcggcagtat attcgggcgg aatgctcatg ccagatgcca gctgacgcac cagcccatcg
     4321 tcactgatgc tgatgcgcgt gataagcccg taaacctcac tttggtcttg caggcgtact
     4381 ttcaccaagg cgccaagcgc gggtgtttcg agttctttca cgcgacagcc agccacaaac
     4441 tggcggttgt ccgcacgcaa aagtttgccg atagggattg tttgtatttc gttcatgctc
     4501 acctcttggg cagtgatttt aaactttgtt tgtgcgacat tgtgccactg ggcaaatgtt
     4561 cgtggtccag gcacgaaaga agcatttctt ccacctcgtc atgttcggca ataacgacca
     4621 ccgccagctc gtgcgcgcgg tgcaaaacgt aagggtaagg tgttttgtcc aacacttgcg
     4681 cgtcgcggta aagaacggcg tgcaagagat taactttttc agggtcttcc gcgacccact
     4741 tggggaactc tacccgtgcc agccaggctc gctcggggtc attgctaacg ttgagtaaaa
     4801 agaaacagac ttcctgtcgt ccttcagcat tttcattttt tgaaacggct tcaaagattg
     4861 cagaccgctc gccgggtgcc aggcgttttt caaagagtgc cagatcgctg acgcctttgt
     4921 tggcgcgctt tttgtcatcg taactttggg aggggtcatt ttttgtttgt acgtaaaggt
     4981 caagcatgcg gtttatcatt tcagcgccgg gtttgtcgat ataaccggcg ttgataatac
     5041 cgcgcctttg catgtgttcg ttgatgactg cgttttgttt ttgcactctt tgacgccgtt
     5101 ccccgcttcg actcgcgccg gtaaagactt ccagcgggcc atccgtaagg gtaatggttg
     5161 gcatgcttgg ctccaggtgc gtggcttcaa gcaaaaactg acgctccaaa aggtcacgat
     5221 gcagggcgac ttcttcttcg ccgatgagaa aaccctcgtt ggtgtagagg tcgtcctggt
     5281 caagcagttt ggtgtagaca ttctgcgttg gcgcgtgccc actgccatgc tcaacatgga
     5341 ttaaaccgat gtttatcagg caaaactgga gcgcgcggtg tcggctgggg ttgatttgcg
     5401 aaccgtcggc ggcaatcagc gtgtattttt caggacaggc cggcaaaggg aaactcgcca
     5461 gcatggcctc atcgcttggc ttggcggtat agaggctttt ggcctgtctt tcaacgcgtt
     5521 caacggcttg gcgcacggca tcttgctcac gggcggctat ttcgatttgc gcggtggcat
     5581 tttgatagtt ttggcgcatt tcgtcaagct ttttccgcgt taaatccgca aaacggtcca
     5641 gattactttc gatgtgggca taattgattg gcataagcag tcctggtcta aaaggaaatt
     5701 gacctgtacc gttattataa gctaaagaca ggctgcaaac agtgattccc gaagcctgta
     5761 atggatagaa aagcttgggt tgtgggaaaa gctggaacaa aatggtgaaa ataatcgttt
     5821 tataagcagg aaactgaata tcttaggagt ataaaatgaa aagcaataaa tggatactaa
     5881 ttataggtgg catacttgct gtggggctaa tgctcaccgc atgtacgcat ccgcaagtga
     5941 caaaagaagt ggaatcaatc agaacgctaa gcgttagtgg cagtgggcag gttacgctcg
     6001 tgcccgatat cgcgcgtgtt aacatcggtg tgcatagcga ggcggaactt gtcacagtcg
     6061 ctgtcaatga aaacaacaaa caagcccagg cgattaccga tgctttgctt aagaaaggca
     6121 ttgaagccaa agacattcaa accgcta
//
LOCUS       DAOSFC010000054         6105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_11358_length_6105_cov_5.800331, whole genome shotgun sequence.
ACCESSION   DAOSFC010000054 DAOSFC010000000
VERSION     DAOSFC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11358_length_6105_cov_5.800331"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1407)
                     /locus_tag="PK791_02360"
     CDS             complement(121..1407)
                     /locus_tag="PK791_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92188.1"
                     /translation="MQDPNNHFPGFEQIPSKLEGVLVFAPIPEKDEAQARSYACPQCG
                     GSLKFDVNSSGMACIYCGYLAPVATAHVGRQAQSFEFTLENVQAAEQEWETDRELLHC
                     NNCGAELSYPKGAIAASCPYCGSNKVNLSLPKTKSLRPQVLLPFSATPEALKPRIREW
                     LSKGWMHPKALKDAAQMDKLTPVYLPYWTFSASIAADWKAEVGHYETERYYNSSSKSY
                     ETRQVLRWRWENGSITHYFSDLMVNGVSPNRLNHTMLNAIEPYNLSGLVLFSPDYLVG
                     MNVQAYETPLNDAWQSAKQQMRADTERLCEQDTGSSHVRNMSIDMSYDDEQWRYVLLP
                     LYLAGYSFAGQYYNIMLNGQTGKLAGNKPVDWKKVRTITWLIALIPLLLALIGFIVMQ
                     ISGNSGLLTFGALASLAGIVVSISLYSQAKKAEKLS"
     gene            1479..1970
                     /locus_tag="PK791_02365"
     CDS             1479..1970
                     /locus_tag="PK791_02365"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOH92189.1"
                     /translation="MHYIIDGHNLIPKIPGLKLSDPEDEKKLIDLLIPFLRLTRSRAR
                     VFFDRATDGQSGERNFGLLKAVFVPAGQSADSAIIAHIHKLAGEARNHSLVSSDRMIQ
                     AAARARYVPIIRSEDFADMLIEKLEEDPERTQAFEGLTVGEVEEWEALFNQYGTTPDG
                     YNP"
     gene            2144..3886
                     /locus_tag="PK791_02370"
     CDS             2144..3886
                     /locus_tag="PK791_02370"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOH92190.1"
                     /translation="MKHTTRKFIILSLLLLMALSACRVEKVVSPTDVPKTQEPTKPSA
                     ETLVPSETPTAFISPTSAPSATPAPPAFWKGTLPLDLQERICLPDGAVWASDENTATY
                     SIVAPVPEKMNTHLASTQWTYALVAPFFTVRDDISLSELQFFLMGKESESPDLSDRKV
                     LLDEGDYDSLIALLGLSNPSDEVLKKVTSDAILADPWGDDWAIVPFDALQPGWKVLAI
                     DGISPLDHDFNPDKYALTLHVTIESKTENNEQSASMDFSGILPVPNRDETQLTSLIMT
                     GVTAMARDTAYVMETQGVLFPGTEIRDILRAADLTHISNEVSFYEGCAFPDPDYKGFI
                     FCSNPEYIKLLDDLGADIIELTGNHNNDVLALYKVDSVPFTLELYEQYGMRWYAGGVN
                     IEDAKAPLLIEHNGNKLAFIGCNSYGPEMAWATESGSGAAPCEDFSWLKAEVARLRAE
                     GYLPIVTFQFQEDYFREASSLQLRDFRDVATAGPVIVNGSQSHVAKAMEFYEGVFIDY
                     GLGNLFFDQPEFYITYDSFIQEHHFYQGRHISTKLYTITIEETGKPRPMTDEERSEFL
                     AIIFSVSEPLRRTQ"
     gene            3883..4848
                     /locus_tag="PK791_02375"
     CDS             3883..4848
                     /locus_tag="PK791_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOH92191.1"
                     /translation="MTSIRDFPTAPLYTIKVVQNRTGIKPVTLRAWERRYELLEPTRL
                     KNGYRLYSERDVQLLLWVKSRLEAGMSISSVVQQFNAMRAKDSWPESIEKLEMEQPRE
                     KSPIPARDYAELLLASMISHNEARMQSLVGDFRKYFDLITIFEEILQPSLALLEHTWF
                     RGDIPLATVHVITQLVKSRLFNVMTAIPVTRENALTLIGSGPGETKELDVLMLTVLMR
                     EAGVFVEYLGPDLLTEDILDYSLIAKPKFICLYIGEEATAYLMRGFASKLAKIPSKPK
                     LLYMGKYLDDNPAEREQLGGIYLGRTLTENLETTRKIMGLGTSSG"
     gene            complement(4966..>6105)
                     /gene="glyS"
                     /locus_tag="PK791_02380"
     CDS             complement(4966..>6105)
                     /gene="glyS"
                     /locus_tag="PK791_02380"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOH92192.1"
                     /translation="RNGDEKNADIVVDGNLQVILARFADAEFFINADREKALEDFVPE
                     LAQLTFQKELGSMLEKTTRIEKLTQDLADLLALDPTQQKTAARIARLCKADLASEMVV
                     EMTALQGVMGRYYARLSGEPQAVAEGIFEHYLPRSADDMLPTQLPATVVGIADRLDSI
                     VGLFAVGLAPTANKDPFALRRAALGLNQILIGRGLDFDLENALLLAARNYDLEISAEV
                     IQNALEFIQGRLHGVLLDKGYRYDVVEAVEQVQASNPAKADRYAELLNNWVQKPEWET
                     LLPAYSRCARITRNISETYALAPEVLQDPAEHALYEALKQTAPNNFEEALQTVEGLVP
                     GINTFFDNVLVMSDDEKVRQNRLALLQKVVGLLSPYADISKLEGF"
BASE COUNT         1564 a         1434 c         1542 g         1565 t
ORIGIN      
        1 tcgctttttg acagatgggg caagccacgt tgtgccaatg cgcgggaaca aagtaatttc
       61 gcgcgcatgc cgggcaagtt tgcgtgagaa atgggtcggg ggtgtttacg gtatgttcag
      121 tcacgatagt ttctccgctt tcttcgcctg actgtaaagc gaaatggata cgacaatacc
      181 ggccaggctt gccagtgcgc caaaggtcag caggccagag ttaccactta tttgcatgac
      241 aataaagcca atcagtgcca aaagtaaagg aataagtgcg ataagccagg ttatggtgcg
      301 gactttcttc cagtccacag gcttgttgcc ggccagtttc ccggtttgac cgttgagcat
      361 gatgttgtag tattgccccg cgaagctata tcctgccaga tataagggta acaaaacata
      421 tcgccactgt tcgtcatcgt agctcatgtc aatactcatg ttacgaacat gactgctgcc
      481 agtatcttgt tcgcaaagtc gctctgtgtc agcacgcatt tgctgtttgg cactttgcca
      541 ggcgtcgtta agcggtgttt cgtaagcttg cacattcatg cccaccaaat aatctggaga
      601 aaaaaggacc agcccgctta agttatacgg ttcaatcgcg ttgagcatgg tgtggttcaa
      661 gcggttgggg ctgacaccgt tgaccatcag gtcagaaaaa tagtgggtga ttgagccatt
      721 ttcccatcgc cagcgtaaaa cttgtctggt ttcgtaagat ttacttgaag agttataata
      781 tcgctcggtt tcgtagtggc caacttccgc cttccagtcc gccgcgatag acgcgctaaa
      841 agtccagtag ggcagataaa caggcgttag cttatccatt tgagcggcat cttttaaggc
      901 cttggggtgc atccagcctt tgcttaacca ttcacgaata cgtggtttga gtgcttcagg
      961 cgtggcacta aaaggcaata agacctgtgg acgcaggctt ttagtttttg gcaggctaag
     1021 attgactttg ttcgacccac aatatggaca actggccgcg atggcgcctt tgggatagct
     1081 aagttcagcg ccacaattgt tgcaatgcag caactcgcgg tcggtctccc attcctgttc
     1141 agcagcctga acattttcaa gcgtaaattc aaaactttgg gcttgtcttc caacatgggc
     1201 ggtggcgaca ggggcaagat agccacaata aatgcacgcc atccctgaac tgttcacatc
     1261 aaactttaaa ctgcccccac actgcggaca ggcataggaa cgcgcctggg cctcatcctt
     1321 ttcgggaata ggcgcaaaga ccaaaactcc ctctaattta gatgggattt gttcaaagcc
     1381 tggaaaatgg ttattagggt cttgcaaaac acacctccgc gtgttgttct tacgctatta
     1441 taatgggaaa agtcaaatgc atcaggtaaa atcaaagcat gcattatatt attgacggac
     1501 acaatcttat ccctaaaatc ccgggtttga aactaagcga ccctgaggat gagaaaaagt
     1561 taattgattt gttaattcct tttttgcgcc tgacacgatc tcgcgccaga gtgttttttg
     1621 accgcgcgac tgacgggcag agtggtgaac gtaactttgg attgctaaaa gccgtttttg
     1681 tgccggctgg acagagcgct gattcggcaa taatcgcgca tatccacaaa ttagcaggcg
     1741 aagcaagaaa ccatagcctc gtgagttccg acaggatgat tcaggcggct gccagggcgc
     1801 gctatgtgcc gattattcgc agtgaagatt tcgccgatat gcttattgag aaactggaag
     1861 aagacccgga aagaacgcaa gcttttgaag gattgacggt aggcgaagtt gaggagtggg
     1921 aagccctttt taatcaatac ggcaccacgc cggacggtta taatccatag aaagaacttt
     1981 ataaaaagct ttatgaagtg cagtgggttt tgtttgcttt ggtatggata tggatgggtt
     2041 tttataaaca agactgaaat cgcgtgactt tttaacaata gcttgttaac cagggagaaa
     2101 gacactataa tttactaaat catttattaa aaagacgatg aaaatgaaac acacgacgag
     2161 aaaatttatc atactgagct tgctgttgct gatggcttta agcgcttgtc gtgttgaaaa
     2221 agtagtgtct ccaactgatg tgccaaaaac acaagaaccc actaaaccga gtgcagaaac
     2281 acttgtgccg agtgaaacac cgaccgcttt catctcgcct acatctgccc cgagtgccac
     2341 cccagcacca ccagcttttt ggaagggtac cctgcccctt gacctgcaag agcgtatctg
     2401 cctgccagac ggcgcggtat gggccagcga tgaaaatact gctacgtatt ccattgtcgc
     2461 accagtgcct gaaaaaatga acacacacct tgcgtcaacg caatggacat atgccctggt
     2521 tgccccgttt tttacggtgc gtgatgacat cagccttagc gagttacagt tctttttaat
     2581 gggtaaagaa agcgaaagcc cagacttatc agatcggaag gtcctgctcg atgaaggcga
     2641 ttatgacagc cttatcgctt tgttgggttt atccaaccca tccgatgaag tgctaaaaaa
     2701 agtcaccagc gatgccattt tggcagatcc ctggggggat gactgggcaa tcgtgccctt
     2761 tgacgctttg caacctggct ggaaagtact tgccatagat ggcatctcgc ccctggacca
     2821 tgatttcaat cctgataaat acgccctaac cttacatgtg acgattgaaa gcaaaactga
     2881 aaacaacgaa caaagtgcca gtatggattt ctcgggtata ttaccagtgc caaaccgcga
     2941 tgaaacccaa cttaccagtc tcattatgac cggtgtcact gccatggcgc gcgacaccgc
     3001 ttacgtgatg gaaacacagg gtgtgctatt tcctggcacc gaaatccgcg atatcctgcg
     3061 cgctgcggat ttgacccaca tcagtaacga agtttctttt tatgaaggct gcgcgtttcc
     3121 cgatccggat tacaagggtt ttatattctg cagcaatccg gaatatatca aactgctgga
     3181 tgaccttggc gcggatatta tcgaactgac ggggaatcac aacaacgacg ttttagcgct
     3241 ttacaaagtc gattcagtac ctttcacgct ggaactttac gaacagtatg gcatgcggtg
     3301 gtatgctggc ggtgtgaata ttgaagatgc caaagctcct ttgctgattg agcacaacgg
     3361 caacaagctg gcttttatcg gctgtaattc atatgggccg gaaatggcat gggcgaccga
     3421 atcgggcagt ggcgcggccc cttgtgagga ttttagctgg ctgaaagccg aagtggcgcg
     3481 cttgcgcgcg gaaggctatt tgcctatcgt cacctttcaa tttcaggaag attatttccg
     3541 cgaagcaagt tccttgcagt tgcgcgattt tcgcgacgtt gccacggctg gcccagtgat
     3601 tgtaaatggc agtcagtccc atgtggcaaa agccatggaa ttttatgaag gcgtctttat
     3661 tgattatggc ttgggcaatc tcttttttga ccagccggaa ttttatataa cctacgacag
     3721 ttttatccag gaacatcatt tttatcaggg cagacacatc agcacgaaac tttacacgat
     3781 taccattgaa gaaaccggca agccacgtcc gatgactgac gaagaacgct ctgaattcct
     3841 ggccattatt ttctcggtct ctgaacccct taggaggaca caatgaccag tatccgtgat
     3901 tttccaaccg cgccattata taccattaaa gtcgttcaaa accgcactgg catcaaacca
     3961 gtcacgcttc gtgcatggga gcgtcgctat gagcttttag aaccaacgcg tcttaaaaat
     4021 ggctatcggc tgtattctga acgcgacgtt cagcttttac tgtgggttaa gagccgtctg
     4081 gaagcaggca tgagcatcag cagtgtggtg caacagttca acgcgatgcg cgcgaaggat
     4141 agctggccag agagtattga aaaactcgaa atggaacagc cacgcgaaaa atcaccgata
     4201 ccggcgcgtg attatgctga acttttgctg gcttcgatga tttcgcacaa cgaagcgcgt
     4261 atgcaaagtt tggtgggtga ttttcgcaag tattttgatt tgatcactat ctttgaggag
     4321 attttacagc ccagtttggc cttgctggaa catacctggt ttcggggtga tattcccctg
     4381 gctaccgtac atgtaatcac gcaactggtt aaaagcaggt tgttcaatgt gatgaccgct
     4441 ataccagtga cgcgcgaaaa cgcactaacc ttgatcggca gtggccccgg tgaaaccaaa
     4501 gaactggatg tcttgatgtt gactgtgctg atgcgcgagg ctggtgtttt tgtggagtat
     4561 ctaggtcccg atttgctgac agaagatatt ttggattact cccttatcgc aaaacccaag
     4621 tttatttgcc tttatattgg cgaagaggcc acagcctacc tgatgcgtgg attcgcgtca
     4681 aagttggcaa aaataccttc caaaccgaaa ttactgtaca tgggcaagta cttggacgat
     4741 aatcccgcgg agcgtgagca attgggtggt atctatttgg gcaggaccct aactgaaaat
     4801 ctggaaacca cgcggaagat aatgggcctt ggaacaagtt caggatgagg gaaaagccag
     4861 gcaaggaatg cttgttgaag taattattgt cttaagcata catatagcaa aaacgctgac
     4921 ataactgcca gcgtctttaa ttatttaatc aactaagtga actctctaaa aaccttccag
     4981 ttttgagata tccgcataag gtgacagtaa gccaaccact ttctgcagca gtgccagtcg
     5041 gttttggcgt actttctcgt catcggacat caccagcacg ttatcgaaaa aggtgttgat
     5101 gcctggcacg agtccttcca ctgtctggag cgcttcttcg aagttgttcg gtgcagtttg
     5161 cttcaaagcc tcatataagg cgtgttcggc ggggtcttgc aagacttcgg gcgcaagggc
     5221 gtaggtttcg ctgatgttgc gcgtgatgcg cgcgcagcga ctgtaagcgg gcaacagggt
     5281 ttcccattcc ggtttctgca cccagttatt cagcagttca gcgtagcggt cggccttcgc
     5341 gggatttgac gcctgtactt gctcaacggc ttcaacaaca tcatagcgat agcccttatc
     5401 caacaagact ccgtgcaggc gtccctggat aaactcaagc gcgttttgga taacttcagc
     5461 ggagatttcc agatcatagt tcctggcagc cagtagcaaa gcgttttcaa ggtcaaaatc
     5521 aagcccacgt ccgatgagaa tttggttcag tcccaaagca gcgcggcgca aggcaaaggg
     5581 gtctttgttg gcggttggcg ccaaacccac cgcgaaaagc ccaacgatgg agtccagccg
     5641 gtcagcaatg cccacgaccg tggcgggaag ttgggtgggg agcatgtcat cggcgctgcg
     5701 gggcagataa tgctcaaaaa tgccttccgc gaccgcctgg ggctcaccag acaggcgcgc
     5761 gtaatatctg cccatcacgc cttgcagggc ggtcatttcc acaaccattt cgctggctaa
     5821 atcagccttg caaagtctgg cgatgcgcgc ggctgttttt tgttgtgttg ggtcaagcgc
     5881 gagtaggtct gccaggtctt gggtgagttt ttcgatgcgc gtggtttttt cgagcatgga
     5941 gccaagttct ttttggaaag tgagctgggc taattcgggc acgaaatctt ccaaagcctt
     6001 ttcgcggtcc gcgttgataa agaattccgc atcggcaaag cgcgccaaaa tgacctgtag
     6061 gttgccatcc acaacgatat ccgcgttctt ttcatcaccg ttgcg
//
LOCUS       DAOSFC010000055         6079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_11418_length_6079_cov_5.453187, whole genome shotgun sequence.
ACCESSION   DAOSFC010000055 DAOSFC010000000
VERSION     DAOSFC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11418_length_6079_cov_5.453187"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1233
                     /locus_tag="PK791_02385"
     CDS             166..1233
                     /locus_tag="PK791_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOH92193.1"
                     /translation="MNKNKSFLSRNKGILIFLAVMLVLPFIIGLFEGSNPVAVWNNQG
                     TFSKHLEGIGIEIFILAIFALSYDLLFGVTGMMSFGHTMFFAVAAYTTGIALKSFGLP
                     LWQVLLIIVGTSIVQAFLFSIVLPRVKGVTFTMVTLGMASMFHIIVMSSDMIKWTGAD
                     VGLQGVPKPAFIDPTIERLRFYVVALLILVLVYIFYKRFIDSPTGRVCVSIRENESRA
                     KMLGYNTTIFKVVVMLIASFTASIAGVLHTLYQPIVSPTIADLGYTVTGLLIVLIGGI
                     ATQSGAIVGAFVIKLLDLALRGIMKDSAPLVTGVVYVIFVLFVPYGIVGSLKLGGFKI
                     KQGWQMLLERFGLKKAMPKEE"
     gene            1404..1676
                     /gene="rpsO"
                     /locus_tag="PK791_02390"
     CDS             1404..1676
                     /gene="rpsO"
                     /locus_tag="PK791_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOH92194.1"
                     /translation="MSITKEEKTDIIEKFATKPGDTGSPQVQIAILSKRISALTDHLR
                     ANKHDESSRRGLLRLVGRRRRLLAYLRQKDYDAFLAITEELKIRRK"
     gene            1865..4120
                     /locus_tag="PK791_02395"
     CDS             1865..4120
                     /locus_tag="PK791_02395"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOH92195.1"
                     /translation="MKPEAILYTATLGGKELSFETGRLARQAGGSIVVRQGDSMMFAA
                     ATLGNVREGVDFFPLTVDYEERMYAGGKIPGSFFRREGRPSTEATLVARLTDRPLRPL
                     FPDGMRNEVQVILMSLSADNENPLDMLAINAASAALMISDVPWAGPVGAVRIAYVDGE
                     LIANPTMQEMEISELDLRVAGSKEAILMVEAGSNEISEDLMAEALQLAHESIQDVIAV
                     QERMAREIGKAKREVSLAASDTELIAQVKSAVKARVTALLDQPYLKSNLYEGMDQIKA
                     EYLLETLGEEPDPAELSRYGKAFDEAQRMIVRDRILFEGKRPDNRKVYEVRDISVETD
                     ISPRAHGSGLFTRGETQVLTLATLGTPKEAQELDNLNGMDSKRYMHHYNFPPYATGET
                     KRVGGQSRREIGHGALAERALEPVLPNEKDFPYTIRLVSEVLESNGSSSMASVCGSSL
                     ALMDSGVPVSAAVSGVAMGLIMEGERYVILTDILGTEDHLGDMDFKVAGTRDGITALQ
                     MDIKIAGITPEIMAEALRRARDARMYILDQMDKVIAEPRPELKSHVPRIETIKVPVDK
                     IGAIIGPSGKNIRALQEETNTKIDIDDDGTVYIAAVDGEGFYEARERILAMTETAEIG
                     RIYTGKCVRVTDFGAFIEILPGVDGMVHISQLSTERVDKVEDVVNVGDDLTVMVIDIA
                     SDGKIRLSRQALLEGWTVEEARERDKPGGSRPRGGSRPGGRSGGDRRSSGGRSGGDRR
                     DRSGGDSRNRR"
     gene            complement(4249..4659)
                     /locus_tag="PK791_02400"
     CDS             complement(4249..4659)
                     /locus_tag="PK791_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH92196.1"
                     /translation="MPPLAHPRQHRLDEIRRDIHAFYQTRLGQDIHCYLVEVDGEVAA
                     IGFLQAHVVAYHPGCPTQRFARITNMLCYEKYRRRGFARQTMEALIALAKDMDFDYIT
                     LDASVMGRHLYESLGFVDYVPVDPPMHLRLRPKD"
     gene            complement(4733..5797)
                     /locus_tag="PK791_02405"
     CDS             complement(4733..5797)
                     /locus_tag="PK791_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92197.1"
                     /translation="MKAFRVAVLANIKTNAPVFEGMSEDQWDDLDSESTVESLCNAIR
                     EGGHEAQFLEADETIIDSIRHYQPDICFNIAESHFGDSREAQIPAILEKLRIPYTGSK
                     VLSLALTLDKPMTKRILAWHGLPTPEFQVFERVDEPLDEGLRFPLFVKPSREGTGMGI
                     SGKSVVHDERELRQQLEISLSKYKQPALVERFIKGREVTVGLVGNLIGPVAWRVPRNE
                     NARRIQAGLNFLPPMEVNLDPTMTIEGVYGNYLKTALADQLEYLCPAPLTEDQIDELN
                     WLAAAVFRVTGCLDVSRIDFRLDIEDNLKPYILEINPLPGLMPKLSDIVIEAEADGIS
                     HTELVNMILNAALKRYGMIE"
     gene            complement(5847..>6079)
                     /locus_tag="PK791_02410"
     CDS             complement(5847..>6079)
                     /locus_tag="PK791_02410"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92198.1"
                     /translation="SKSAEFYGFFSVFEKFNTVVGPALLAVITKLTGSDRYGIISLVV
                     FFLAGIIMLQFVNIERGVKVAEAEDKLLVQLD"
BASE COUNT         1503 a         1402 c         1577 g         1597 t
ORIGIN      
        1 attcaacaat ttatgatcaa atacggtcaa attggtatcc cgataccttt caccgatatt
       61 atctttaaac ctaccccggc aattattcca gcatccacca tgctcttgat ggtgattgtg
      121 ttgctcattt tgccgaacgg acttttggga aggcaggact aagctatgaa caaaaacaaa
      181 tcattcctaa gtcgaaataa gggcattctg attttcttag cggtgatgct cgtgcttccc
      241 ttcatcattg ggctctttga aggctcaaac ccggtggcag tctggaacaa tcagggcacc
      301 ttttcaaagc atttagaagg catcggaatt gaaatcttca ttcttgccat cttcgccttg
      361 agctatgacc tgctttttgg tgttaccggt atgatgagtt ttggacatac catgttcttt
      421 gctgtcgcgg cttatacaac tggtatcgcc ttgaaatcat tcggcttgcc tctgtggcag
      481 gtgctgttga ttatcgttgg cacttcaatc gtgcaggcgt ttttgtttag cattgttctg
      541 ccacgtgtca agggtgttac ctttacgatg gttacgcttg gtatggcatc catgttccat
      601 atcattgtta tgtcttccga tatgatcaag tggactggtg ctgatgttgg tttgcaaggt
      661 gttccaaaac ctgcctttat cgatccaacg attgagcgct taaggtttta tgttgtcgcc
      721 ttgctcatat tagttctcgt gtatatcttc tataagcgct ttattgactc gcccaccgga
      781 cgtgtgtgtg tttcgattcg cgagaatgaa tcacgcgcga agatgctagg ctataacaca
      841 accatcttca aagtagttgt catgttgatt gcttcgttta ctgcttcaat agctggtgtg
      901 ttgcataccc tttatcaacc gattgtcagt ccgactattg ccgaccttgg ctatacggtt
      961 acgggcttgt tgattgtgtt gattggcggt attgccaccc agagcggggc gattgttggt
     1021 gcttttgtca tcaagctgct tgatttggca ctgcgtggca tcatgaaaga cagcgcgcca
     1081 ctggttaccg gcgttgtgta cgttattttc gtgctctttg tgccttatgg catcgtgggt
     1141 agcttgaagc tcggtggatt taagataaaa caaggctggc agatgcttct tgaacgtttt
     1201 ggtttgaaaa aggcaatgcc aaaagaagaa taacagcctg caatctctaa aaaatacaaa
     1261 tttactctat aaagctcttg cgtaagcagg agcttttcgt gtataataac aagttgtgac
     1321 tggtcgggtg agcggttgat gagtaattac ccatcggctt cgacccagga gacctgcata
     1381 aaaaatattt aggagcagac gtcatgtcaa ttacaaaaga agaaaaaact gatattatcg
     1441 aaaagttcgc caccaaacct ggcgatactg gttcacccca ggtgcaaatt gcaatcctgt
     1501 ccaagcgcat ttccgcgctt actgatcacc tgcgtgcaaa caagcacgac gagagttcac
     1561 gccgtggttt gcttaggctg gttggtcgcc gacgccgttt gttggcttat ttgcgccaaa
     1621 aggactatga tgctttcctg gcaattacag aagagctgaa gatccgcaga aagtaatatc
     1681 gttgtcaaat gatacccgtt acagcgcacg ccctgacaca gaatgtaagt aagagtaatg
     1741 tcagtgcgtg ctctgttgtg gtatcaataa aaattatcaa gccagtcggg tattgaaatt
     1801 tagcgggcaa attgtttgac ctttaaattt cagttcctgc actggctaac agtgaaagga
     1861 aaatatgaaa ccagaagcaa ttctctatac agccacgctt ggtggcaaag aacttagctt
     1921 cgaaaccggc cgtttggctc gccaggcggg tggttcgatt gttgtacgcc agggcgacag
     1981 catgatgttt gctgccgcga cacttggcaa tgtgcgtgaa ggcgtggatt tcttcccgct
     2041 gaccgttgat tatgaagaac gtatgtatgc cggaggcaaa atcccaggtt cgttcttccg
     2101 ccgcgaaggg cgccccagca ccgaagctac tttggtcgcg cgcttaactg accgtccctt
     2161 gcgcccgctc ttccccgatg gaatgcgcaa tgaggtgcag gttatcttga tgtcactttc
     2221 cgcagataac gaaaatcccc tggatatgct tgccatcaat gccgcctccg ccgcactgat
     2281 gatttcagat gtgccctggg ctggaccagt tggcgcagtg cgtatcgctt atgtggatgg
     2341 tgaactgatt gccaacccga ccatgcagga aatggaaata tccgaacttg atttgcgtgt
     2401 ggctggcagt aaagaagcta tcttgatggt cgaagctggc tcaaatgaaa tttctgaaga
     2461 cctcatggca gaagctttgc aactggcaca tgagagcatc caggacgtca tcgccgttca
     2521 ggaacgcatg gcacgcgaaa ttggcaaagc caagcgcgaa gtgagtcttg cagccagcga
     2581 tacagaactg attgcccagg taaaatctgc tgttaaagca cgcgttaccg cgcttttaga
     2641 ccagccttat cttaagtcca acctttatga aggaatggat caaattaaag ctgaatatct
     2701 gcttgaaacc ttgggcgaag aacctgaccc tgctgaactc agcagatatg gcaaggcatt
     2761 tgacgaagcc cagcgcatga ttgtgcgtga ccgtattctg ttcgaaggca aacgccctga
     2821 caaccgcaag gtctacgaag tgcgcgatat cagcgttgaa acggatattt cgccccgcgc
     2881 gcatggctcg ggtttgttta cccgtggcga aacacaggtg ctcaccctgg ccactttggg
     2941 cacacccaaa gaagcgcagg agttggacaa ccttaacggt atggacagca aacgctatat
     3001 gcatcattac aatttcccac cttacgctac tggcgaaacc aaacgtgtag gtggacagtc
     3061 acgacgtgaa atcggccatg gcgctcttgc tgaacgcgcg ctggaaccgg ttttgcctaa
     3121 cgaaaaggac tttccttaca ccattcgttt ggtttcggaa gtcttggaat caaacggttc
     3181 gagttcaatg gcctcggttt gtggttcaag cctggcatta atggacagtg gtgtccccgt
     3241 aagcgcggcg gtttccggcg tggcgatggg tctcattatg gaaggcgagc gttacgtgat
     3301 tctcactgat attcttggaa ccgaagatca tcttggtgat atggatttca aggtggctgg
     3361 tacgcgcgat ggcattaccg ctttgcaaat ggacatcaaa attgctggta tcacgcccga
     3421 aatcatggct gaagctttga gacgtgccag ggatgcccgt atgtatatcc tggaccaaat
     3481 ggataaagtc attgctgaac cacgccctga attgaaatcc cacgttccac gtattgagac
     3541 cattaaggtg cctgttgata aaattggcgc gattattggg ccatccggta aaaatattcg
     3601 tgctttgcaa gaagaaacca acacgaaaat tgacatcgat gatgacggta ccgtttacat
     3661 cgctgctgtt gatggtgaag gtttctacga agcgcgcgaa cgcattttgg caatgaccga
     3721 aacagctgag attggacgca tttacaccgg taaatgtgtg cgcgtgaccg attttggcgc
     3781 attcattgaa attttgcccg gtgtcgatgg catggtgcat atcagccagc tcagcacaga
     3841 acgcgttgac aaggttgaag atgtggtgaa cgttggcgat gacctgactg tgatggtgat
     3901 tgatatcgcc tcagacggca agattcgtct ttcgcgccag gcccttttgg aaggctggac
     3961 agttgaagaa gcccgcgaac gcgataagcc aggtggctcc cgcccgcgtg gtggttcacg
     4021 accaggtgga cgctccggtg gtgatcgccg cagtagtggt ggtcgctccg gtggtgaccg
     4081 tcgcgaccgc tcgggtggcg atagtcgtaa cagacgataa accgtaaccg taactagtta
     4141 aaagaacaaa ccgctttggg caaccaaggc ggtttgtgtt ttgcctggtg ttttaatgta
     4201 aaaaaagtgc atggcatcta tgccatgtac cagtaaacga atgacgcgct aatctttcgg
     4261 tcgcagacgc agatgcatgg gcgggtcgac gggaacatag tcaacaaagc ccaggctttc
     4321 gtaaaggtgg cgacccatca cggacgcgtc aagggtgatg tagtcaaaat ccatatcttt
     4381 ggcaagggcg atgagagctt ccatcgtctg gcgcgcgaaa ccacgccggc gatatttctc
     4441 atagcacagc atgttggtaa tacgcgcgaa acgttgtgtg gggcagccag ggtggtaggc
     4501 gacaacatgc gcctgcaaaa agcctatcgc ggcgacttca ccatcgactt cgaccaaata
     4561 acaatggatg tcttgcccca agcgcgtttg ataaaacgcg tgaatatcgc gtctgatttc
     4621 atccaggcga tgctggcgcg ggtgtgccaa gggcggcatt gaagtcaatc cgcccttgaa
     4681 caagtttgtc taaatctgcg tgagttgcga aatgtccggt aatgttcata ctttattcta
     4741 tcatgccata gcgcttgagc gcggcattga gaatcatatt gaccaactcg gtgtggctga
     4801 tgccatccgc ttcagcttcg atgacgatgt cggaaagctt gggcattagg ccaggcaagg
     4861 ggttgatttc caggatgtag ggtttgagat tatcctcgat atccaggcgg aaatcaatgc
     4921 gcgatacgtc caaacaaccg gttacacgaa agaccgcggc ggcaagccag ttgagttcgt
     4981 cgatttggtc ctcggtaagt ggcgcggggc ataagtattc cagctggtct gccagggcgg
     5041 ttttaagata gttgccgtaa accccttcaa tggtcattgt ggggtcaagg ttgacttcca
     5101 tgggtggcaa aaagttcaag ccagcttgaa tacgacgcgc gttttcatta cgcggaacgc
     5161 gccaggcaac ggggccgatc agattaccca ccaaaccaac ggttacttcg cggcctttga
     5221 tgaaacgttc aaccaaagcg ggctgcttgt atttgctgag acttatttca agctgttgac
     5281 gcagttcgcg ttcatcgtgc accaccgatt taccgctgat gcccataccg gtaccttcgc
     5341 gactgggttt gacaaaaagc gggaatctta agccttcgtc aaggggttca tcaacgcgtt
     5401 caaaaacctg aaattctggt gttggcagtc cgtgccaggc gagaatgcgt ttggtcatgg
     5461 gcttgtccaa agtgagggcg agcgataaaa cctttgagcc tgtgtaaggg atgcgcaatt
     5521 tctcgagaat ggcaggaatt tgagcctccc gcgaatctcc gaagtgactc tcagctatat
     5581 tgaaacaaat atcgggttgg tagtggcgga tggagtcgat gatggtttcg tcggcttcta
     5641 aaaattgcgc ttcgtggcca ccttcacgaa tggcgttaca cagggattca acggttgatt
     5701 cggagtcgag atcatcccat tgatcttcgc tcatgccctc aaaaacgggc gcgtttgtct
     5761 taatgtttgc aagaactgca acgcgaaatg ctttcacttt tttacatgcc tccggcacga
     5821 atatttttat taattatagt cctaacttaa tcaagttgca ctaacaattt gtcttcagct
     5881 tcagcgactt ttacgccacg ttcaatgttg acgaattgca acatgatgat gccagccagg
     5941 aagaatacga ccagggagat gatgccatag cggtcggatc cagtgagttt ggtaatcact
     6001 gccagcagag cagggccaac cacagtgttg aatttctcaa agacactaaa gaatccgtaa
     6061 aattcagcac ttttactgg
//
LOCUS       DAOSFC010000056         6068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_11444_length_6068_cov_5.441543, whole genome shotgun sequence.
ACCESSION   DAOSFC010000056 DAOSFC010000000
VERSION     DAOSFC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11444_length_6068_cov_5.441543"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PK791_02415"
     CDS             <1..580
                     /locus_tag="PK791_02415"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOH92199.1"
                     /translation="VIEPNTLGSFGYDDDGVPAQDNLLIDKGILVGAITGRQMVEEAN
                     ATERKIIFEGSSGANRASSFYRVPIERMTNINVDPGTDGNLDDIIANTERGIILSGDK
                     SWSIGSNREQFHFANHIGWMVEDGKRTHVVKNPTYRGETVQFYNSLSAVGDESTWHVT
                     YVDNCGKGQPSQIMELGHGVPVCKFDDVIVGD"
     gene            582..1967
                     /locus_tag="PK791_02420"
     CDS             582..1967
                     /locus_tag="PK791_02420"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOH92200.1"
                     /translation="MKSKVTTLLKTLRAYAIEKGIRLAINYHEEDSYLMRFANSAISL
                     NTNERLTRLDLAAYDGRQRAEFQMIADPNDLDSIKKAIDTVGEMVQHATPLNYDPTFP
                     VYQQDVYDDRAYCSNLAKLSNAERLAYFNAVAGDLESNDIKLSGNFANGTTINAQIST
                     ETDHVQFFVSTDAQILVVLSSLSLKWEVNAEQSAQRKRDLDPQGMHEQLALLLDHYKN
                     AEAVQLPVGKYKVVFGPAAVGEVMNMMVGYAMNGSMVKMGYSFLSPDDIGKQVFSPKL
                     SVVDDPNRTEVFCQMADENGFIRQPFSFVEDGVFKGFAWGQDAADEYDQEPTGHSVPH
                     YSLVVSVGDKPVASLKELLEMPRDEDILYIPYIHYMNMVNPSAGVFTGSSRFGALYLK
                     ADGSVQVPYNVRLTQKFTDMFGERVEWMSSKETVYNVSSTYSRRNPEALAIPKYICVK
                     DIEISHSNPSY"
     gene            complement(2206..2403)
                     /locus_tag="PK791_02425"
     CDS             complement(2206..2403)
                     /locus_tag="PK791_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92201.1"
                     /translation="MDKKSAACAALFSQLFCLEILQDISQGQYHESNNQKNYAKGEGD
                     QTSQERHKLEEDSEDKHDNSN"
     gene            complement(2431..3579)
                     /locus_tag="PK791_02430"
     CDS             complement(2431..3579)
                     /locus_tag="PK791_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOH92202.1"
                     /translation="MTRAFGIDISKWQSSADGKRKMDFDAVTKHKEPVSFIAARAGIA
                     DDYEDSMFQYYWKEMKRIRSNRIAYHVLYFGINAVAQMDNLFGILKGKVDWNHDRIAL
                     DLEITDISQRGQYTQTTLDCLEICKQRTGRYPIAYSRAGWVNSHLNINDLPELDWWLA
                     YYLRPNEYPDYTPEHPGPPLLPNGVSQYLIHQTSEKGKSIGAIGHYMDYNRWNGDENS
                     VAAYFGGPGSVAQDEDTEIPDVTAQNQGLFQAQCMVFILFTRTGPSNTMPIIGRLRLG
                     DVVTVFEEKDGWFRVHDKDSIWVQGKRFLMRRIAQHGAKISPLFVARVIVPAQYIRVG
                     PGKEHPVMGNLVKNQLVEVFEERNGWFRIAPGAEVWVHGGAQYLQRVH"
     gene            complement(3771..4625)
                     /locus_tag="PK791_02435"
     CDS             complement(3771..4625)
                     /locus_tag="PK791_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH92203.1"
                     /translation="MEIIGTILRVVLAIVLVTFSIFPVLWIVSAAINPVNDLANQKLI
                     PDNANFDNFTKLVNNPIFPFFTWMKNSLFVSVVSTLFTVSLTTLAAYAFSRFRFKGRQ
                     GLLKSILLVQSFPNLLAIVALYSIINQMGDLPIIGKFLGLNTHWGLILVYMGGAMGMN
                     IWLMKGYMDTIPRDIDESAKVDGATDWQIFSQLLLPLLRPILVVIAILSFIGTYGEFL
                     LARTFLTTSEKQTVMVGLQIFTAGQYTKNWGVFAAGALIAALPIMIIYLVLQDQIVGG
                     LTAGSVKG"
     gene            complement(4648..>6068)
                     /locus_tag="PK791_02440"
     CDS             complement(4648..>6068)
                     /locus_tag="PK791_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH92204.1"
                     /translation="AFFVPKAHMFKWMAFGLAAWLLFSIFPILYTVYNAFTNYGDGHL
                     ISKEQAIQQIESETYLPPDSRAFVSWVFYKGSGNDYLLWVKDDAGETMMARMLKSHEE
                     PKVFDVVSGENGIGEIDANGAPYTIEGYERLSGIAASTDANLTKYRFGEEESPIFIRP
                     DLSVGQFKQLYIYNPADNTFTSQKTGLVYKDVKGTWINSKNQKIIPGYTAGVGFDNFV
                     QFVNSPGMRGPLATIVTWNFAFATLCLLTTFALGLLISIIYNEPKMKGKKLLRSLLII
                     PYTIPSLITILIWRAMFNQEMGIINKVLLEVFNIAPRWYTNPWLARTALLIVNLWLGY
                     PYWMLVTSGALQGIPTDIYEAAQIDGANGWQRFTKITLPLLLVSVGPLMIASFIFNFN
                     NFNLIYAFIGGGPPIPTATTAAGYTDIIISYVYNLAFASGRGAQYGYASAISLVLFVL
                     IAFMSLIQFRFTNMWEETGENV"
BASE COUNT         1682 a         1381 c         1459 g         1546 t
ORIGIN      
        1 tgtcattgag ccaaacactc tcggtagctt tggctatgac gacgacggcg ttcccgcgca
       61 ggacaatttg ctcatcgaca aaggcatctt ggttggcgcg attactggtc gccagatggt
      121 tgaagaagcc aacgccaccg agcgaaaaat catctttgaa ggttccagtg gcgctaatcg
      181 tgccagcagt ttctaccgag tgcccatcga gcgtatgacc aacatcaacg tcgatcccgg
      241 cacagatggt aacctggacg atatcatcgc caacacggaa cgtggcatta tattatcggg
      301 tgataagagc tggtcgattg gttcaaatcg tgagcaattc cacttcgcca accacatcgg
      361 ttggatggtt gaggatggca agcgcacgca tgtggtcaaa aacccaacct atcgtggcga
      421 aacagtgcaa ttctacaatt cgttaagcgc ggttggcgat gaaagcacct ggcatgtgac
      481 ctatgtggat aactgtggca agggccagcc cagccagatt atggaactgg ggcatggcgt
      541 accggtttgt aaatttgatg atgttatcgt gggagattaa gatgaaaagc aaagtaacaa
      601 cgctactaaa aactttacgc gcctacgcga ttgaaaaagg cattcgcctg gcaattaact
      661 atcatgaaga agacagctac ctgatgcgtt tcgcgaactc ggcgatatca ctcaacacca
      721 acgaacgatt aacacgtctt gatcttgcgg cttatgatgg cagacagcgc gcggaattcc
      781 agatgatcgc tgacccgaat gaccttgata gcatcaagaa agcgattgac actgttggcg
      841 agatggtgca acacgccacg cctttgaact acgaccccac tttcccggtc tatcagcagg
      901 atgtttatga tgaccgtgcc tattgctcaa atttggcgaa gttgagtaat gccgaacgct
      961 tggcgtattt caatgctgtt gctggtgacc tggaaagcaa tgatattaaa ctttccggca
     1021 actttgcgaa tggcacaacg attaatgccc aaatcagcac tgaaacagat catgtgcaat
     1081 tttttgtaag cactgacgcg cagattttgg ttgtattaag ttccttgagt ctcaagtggg
     1141 aagtcaacgc tgagcaaagc gctcaacgca aacgcgacct tgacccacaa ggtatgcatg
     1201 agcaattagc ccttttgctg gatcactaca aaaatgcaga agcggtgcaa ttgccagtag
     1261 gcaaatacaa agtggtcttt ggccccgcgg cagtgggtga agtgatgaat atgatggttg
     1321 gctatgccat gaacggaagc atggtgaaaa tgggttattc tttcctgtcg ccggatgaca
     1381 tcggtaagca agtcttttcg ccaaaactga gtgtggttga tgaccccaac cgcactgagg
     1441 tattttgcca gatggctgat gaaaatggct ttatccgcca acctttcagt tttgttgaag
     1501 atggtgtctt taagggcttt gcctggggac aagacgcggc ggatgagtac gatcaggaac
     1561 caactggcca cagtgtgccc cattatagtc tggtggtgag cgtgggcgat aagcctgtgg
     1621 caagcttgaa ggaactgctt gaaatgccac gcgacgaaga tattctctat atcccctata
     1681 tccactacat gaacatggtc aaccccagcg cgggcgtttt tactggttct tcacgttttg
     1741 gcgctttgta tctcaaggca gatggcagcg tgcaggtgcc ttacaacgtg cgcctgaccc
     1801 aaaagtttac cgatatgttc ggtgaacgtg tggaatggat gagctccaaa gaaacggtat
     1861 acaacgtttc cagtacctac agccgtcgca acccggaggc tttggcgatt ccgaagtata
     1921 tttgcgtgaa ggatatcgag atttcacact caaatccatc gtattagttc gaagattgtt
     1981 aagagctaaa agcccgggat aatcctgggc ttttttagtt aagattgatt gtcatagaac
     2041 aggtaatgcg tgatgtatct taaaacagtc aaaaaatatt tttgaaaata aaggttgaca
     2101 cctcaacgaa tcgggttaaa attaaactaa cacaatatgg cttacaaaag ccgctttttc
     2161 ataaggagaa aaatgaaact taatgcccca actaaacttg ctttcttaat tgctgttatc
     2221 gtgtttgtcc tcggaatcct cttccaattt gtgcctttcc tggctggttt ggtcaccttc
     2281 ccccttggcg taattctttt gattgttgct ttcgtgatac tggccttggg aaatatcctg
     2341 aagaatttct aaacagaaca attgagaaaa aagtgctgca cacgcagcac tttttttatc
     2401 cacaaaaata atcctaaaaa acagccctaa ttaatgaacc ctctgcaaat actgcgcgcc
     2461 gccatgcacc caaacttcgg caccgggagc gatgcggaac cagccgttac gctcttcaaa
     2521 cacttcaaca agctgattct tgaccaggtt tcccataacc ggatgttcct tgccgggccc
     2581 cacgcgaata tactgggcag gcacaatgac gcgtgccaca aacagggggc tgatttttgc
     2641 gccgtgctga gcaattcggc gcatcaaaaa ccgttttcct tgcacccaaa tggaatcctt
     2701 gtcgtgcaca cggaaccagc cgtctttttc ttcaaacacg gttactacgt cgcctaagcg
     2761 cagtctgccg attattggca tggtgttgct ggggccggta cgcgtaaaaa ggataaagac
     2821 catgcactga gcctgaaaaa gaccttggtt ttgcgcggtt acatcgggaa tttcggtatc
     2881 ttcatcttgt gcgacgcttc cagggccgcc aaaataggct gccacagagt tttcgtcgcc
     2941 attccagcgg ttatagtcca tgtaatgacc aatcgcgcca atacttttgc ccttttcgga
     3001 ggtttgatga attaaatact gactcacgcc atttggcaac aaaggtggcc cgggatgttc
     3061 gggagtgtag tcgggatatt cgttaggtcg cagataataa gccagccacc aatccagttc
     3121 aggtaaatca ttgatattta aatgactgtt cacccagcca gcgcgagaat aggcgatagg
     3181 gtagcgtccc gttcgctgtt tgcaaatttc caagcagtca agtgttgttt gggtgtattg
     3241 accgcgttgg ctaatatctg ttatttcaag gtcgagtgca atgcggtcgt ggttccaatc
     3301 cactttaccc tttagaatcc cgaaaagatt atccatttgc gcaaccgcgt tgatgccaaa
     3361 ataaagcaca tgataagcga tgcgattgct tctgatgcgt ttcatctctt tccagtagta
     3421 ttggaacatt gaatcttcat aatcatccgc gatgccagct cgcgcggcaa taaaactaac
     3481 aggctcttta tgctttgtta cagcatcaaa gtccattttg cgcttgccat ctgcgctgga
     3541 ttgccattta ctgatgtcga ttccaaaagc ccgtgtcata acactccaaa ataccggcaa
     3601 ttaattgcca gataatgcac aaaaatgggt gcaatttggg tgccaggcag tattgacgat
     3661 gtggctggca gcacggaaaa acaaaacgcg taaacaaaaa acaagcccgt tgagaacacg
     3721 ggcttgtttt tataaattat tagcaggtgg cgggataatg tctaaataga ctatcctttc
     3781 acagatccag ccgttaaacc acctacaatt tggtcttgca atactaggta gataatcata
     3841 atcggcagag cggcaattaa cgcgccagcc gcaaagacac cccagttctt ggtgtattga
     3901 ccagcggtaa agatttgcag gccgaccatc acggtttgtt tttcgctggt ggtcaggaat
     3961 gtgcgcgcca gcaagaactc accataggta ccaatgaaac tcaaaattgc gattactacc
     4021 aatatgggtc gcaggagcgg taataagagc tgactgaaga tttgccagtc tgtggcacca
     4081 tcgacctttg ccgattcgtc aatatcgcgt ggaatggtgt ccatatagcc tttcatcagc
     4141 caaatgttca tgcccattgc accacccatg tagaccaaaa ttaaacccca gtgagtgttc
     4201 aggcccagga atttgcctat tatgggcaag tcgcccattt ggttaatgat gctgtaaagc
     4261 gcgacgatag ccaaaaggtt ggggaagctt tgcacaagta aaatagactt gagcaaacct
     4321 tgtcgccctt taaagcggaa acgtgaaaac gcataggcag ccagggtcgt gagcgatacc
     4381 gtaaatagtg tggaaacaac tgagacgaac agggaatttt tcatccaggt aaagaagggg
     4441 aaaatcgggt tgttcaccag cttagtaaag ttatcaaagt tggcattatc tggaataagc
     4501 ttttgatttg ccaggtcgtt tacggggtta atcgccgcag aaacaatcca cagtacaggg
     4561 aagatactaa aggttaccag cacgatcgcc aacacaacgc gcagaatagt gccgataatt
     4621 tccattgttc tcatagacgt atatttttta gacgttttca ccggtttcct cccacatatt
     4681 tgtaaagcgg aattggataa gcgacataaa ggcgatcagg acaaacaaga ccagcgatat
     4741 agctgaagca taaccatatt gcgcgccacg tccgctggca aaggccaggt tgtaaacgta
     4801 gctgatgatg atatcagtgt atcctgccgc ggttgtcgct gttgggatgg gtgggccacc
     4861 cccaatgaag gcgtaaatca gattgaagtt gttaaagttg aaaatgaacg aagcaatcat
     4921 caaaggtccc acactcacca gcaacagggg cagagtaatt ttcgtgaagc gttgccagcc
     4981 attggcaccg tcaatttgag cagcctcata aatgtcagtt gggatacctt gcaaagcgcc
     5041 actggtgact aacatccagt acggatagcc cagccagaga ttcacgataa gcagcgcggt
     5101 acgtgccagc caggggttgg tgtaccatct tggcgcgatg ttgaaaacct caagcaagac
     5161 cttgttaatg atgcccattt cctggttaaa catagcacgc caaatgagga tggtaatcag
     5221 gcttgggatc gtgtagggga tgatgagcaa actccgcaga agctttttac ctttcatttt
     5281 tggctcgttg tagatgatgg atatcaataa gccaagagcg aaggtcgtaa gaaggcaaag
     5341 tgtggcaaag gcgaagttcc aggtcacaat ggttgccaat gggccacgca tgccagggct
     5401 gtttacgaat tgcacaaagt tgtcaaaacc cacacccgcg gtgtagccag ggatgatttt
     5461 ttggtttttg ctgtttatcc aggtgccttt tacgtcttta tagaccagac cggttttctg
     5521 actggtgaag gtgttatcgg cagggttgta aatgtaaagt tgtttaaatt gtcctacgct
     5581 aaggtcaggg cggataaaaa tgggtgattc ctcttcgcca aagcgatatt tggttagatt
     5641 ggcatctgta gaagcggcga taccacttaa acgctcataa ccttcaatag tatacggcgc
     5701 gccgttggca tcaatctcgc caataccatt ctcaccagat acgacatcga aaacttttgg
     5761 ttcttcgtgg ctttttaaca tgcgtgccat cattgtctca ccagcatcat ctttaaccca
     5821 cagcaaatag tcattcccgg agcctttgta gaaaacccag cttacaaaag cgcgactatc
     5881 cggcggtaaa taagtttcag attcaatttg ctggatagcc tgttctttgc taatcagatg
     5941 gccatcacca tagttcgtga aagcattgta gacagtgtaa agaattggga aaatagaaaa
     6001 caaaagccag gctgccagtc caaacgccat ccacttgaac atatgtgctt taggcacgaa
     6061 aaaggcaa
//
LOCUS       DAOSFC010000057         6066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_11455_length_6066_cov_4.465813, whole genome shotgun sequence.
ACCESSION   DAOSFC010000057 DAOSFC010000000
VERSION     DAOSFC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11455_length_6066_cov_4.465813"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PK791_02445"
     CDS             <1..955
                     /locus_tag="PK791_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92205.1"
                     /translation="LGFEPEIFLVSAKKAQKAQHATEPGEKQQLREESGIATLENFIK
                     NTLDDKSRLHQKLLNPLGVARKVLESANALNSTQLNELSAEIELVESLEALINKHEHD
                     LRLELPPRLAEVDNILNDFEKRGLDFFDSKLKISNIFNLAKPDKIKLEFQDEVQADVA
                     SEIENKVRELIDWTVDKDLELWYRIAGDLERRNEETKLLAGKRSVSEQTERRNELINK
                     ASQSIQTVVSGFDRKKQVEELGDLVQDSVAKTALFGVGAAGIGVLVATVITARALDIT
                     GIVSAGTLAVLGLFVIPYKRKQAKDNFSERMAEMRASLRRP"
     gene            1169..2278
                     /locus_tag="PK791_02450"
     CDS             1169..2278
                     /locus_tag="PK791_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92206.1"
                     /translation="MQRTKTTLAKAILLLLMLSMIFVACDKQNGVDPTDGTEQQATLS
                     EPLANETPEPEATATRDMRGVTQVVLGGDLPDDPVPTWTQSAILTQEALANQTPTATL
                     PGTVVVGEQHYVVYQGGYAVTLDLDAHVSVRGNLSLIGSRTHPVNTLIMGNVSKYEII
                     SVDELPQQVFDSLFGSEAQVSQEEVEEFELDGVQGIALNYIARINQMIAKGRLVVVKP
                     TEKRYVAIIGMGDPADPSGDQWIADGVEFFDAIVNGVEILDDDLLVDNTVCPMATDES
                     YGFSPDNPIRVGGDAIGGPARARAYLDNLQDSKGNWLAYERKGSMEHGGAIVDAYVVI
                     LGGVERLIYINQYDYAELLTPVGFSCGGAYPLVAP"
     gene            2539..>6066
                     /locus_tag="PK791_02455"
     CDS             2539..>6066
                     /locus_tag="PK791_02455"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92207.1"
                     /translation="MLQKIARFFTGDPNKKAIDKLSLIVEQINALEPQFEALSDEALT
                     AKTDEYKARLAKGETLDDLLPEAFATVREASKRVLGQRHYDVQLICGINLHHGSISEL
                     RTGEGKTLSATLPLYLNALEGKGAHLVTVNDYLARRDGRWMGAIYRFLGMSTGILQSA
                     SADGASGRAFIYDPDESSLYEETHQLRPVHRKYAYAADITYGTNSEFGFDYLRDNITM
                     RWDDRVQRPHHFAIIDEVDNILIDEARTPLIISGPSHEDSENYYRMAQVVKALKPEDY
                     EVSEKDHSVALTEIGVAHVEELLGEPLSDPERPEDILPEQARLLGFLEQSLRAQFLFH
                     RNKEYIVQGGEVVIVDEFTGRMMPGRRWSDGLHQAVDAKEGVKVQAENITHATVTIQN
                     YFRMYTKLAGMTGTAMTEKEEFYRIYGLDVLAIPSNLEYKAERADSGIKHAQAQDEFG
                     YDYTYYYAADDPTQEPLYYKRKDYPDVIYRTGEGKLRAIVLEILRYHAIGRPQLVGTT
                     SVESSEHLSSRLSSDMVRRVVQVSLIRRAWRLSRGAKEEDYQQAPELEGLNAPLDKLR
                     MPELRRLGTQFGVSTMDMTDESNREGILELLQLEPEHWERLKPTFDAGIPHQVLNARK
                     HTEESIIIAKAGALGAVTIATNMAGRGVDIKLGGELPENLLSELNQILAKNRDLDPYN
                     MTMPQRLEAVNKLDLDLEEEQENTVDAFKQYMEQMALVREIGGLHVIGSERHESRRID
                     NQLRGRAGRQGDPGSSRFYLSLDDELMRMFGGDRMEGVLSRLKLDESMPIEVGLINRM
                     VEQAQTRVEGTNFDIRKHLLEYDDVLNTQRNRVYEQRDRIFLKEDLHDDIDEMIRNEI
                     APKVELAMADAEGPWKLLAQLEDIQSMINTEFGIYPSYTIKLVADELEDVQDLGTLKS
                     RMLDLAKDAVKQENEHLLEGVELLLARNQETIKNQIAERSEALDAFLETFDPNEIRDV
                     QQELSSLMGVQVRLAAAEQRILRDDPMSMKQPLRDALRTGVTLNVIRRTLMTLEKRFG
                     EVWPLKPIDLASQPWAETQNAILDQAQDTLNHRNTVLFGDNGEVPRDIDNNEALLSEA
                     LEDDEALYRAIQMTMMGKRVGFDARSRQRQIRTHMRLNYIFSMAEKIRKMNVEALTED
                     ILTHLDSAEEHLAEIFGMADWMNVMHKNRV"
BASE COUNT         1644 a         1461 c         1607 g         1354 t
ORIGIN      
        1 tttaggtttt gaacctgaaa ttttcctggt gtcagcaaag aaagctcaaa aagcacaaca
       61 tgctaccgag cctggcgaaa aacaacagct gcgcgaagaa agtggcatcg ctacgcttga
      121 aaacttcatc aaaaacacgc tggatgacaa aagccggctc catcaaaagt tgctcaatcc
      181 cctgggcgtg gcgcgaaaag tgcttgaaag tgccaacgcg ctcaacagca cgcaactcaa
      241 cgaactgagt gccgaaattg agctcgttga atcgctggaa gcgctcatca ataaacacga
      301 acacgatttg cgcctggaac tgcccccgcg gcttgctgaa gttgacaaca ttttgaatga
      361 ctttgaaaaa cgcggactgg atttcttcga cagcaagctg aaaatcagta atattttcaa
      421 tcttgctaaa ccggacaaaa tcaaactcga atttcaggat gaagtccagg cggatgtggc
      481 cagcgaaatc gaaaacaagg tacgcgaact catcgactgg acggtcgata aagaccttga
      541 actttggtat cgcatcgcgg gtgacctgga acgtcgcaat gaagaaacaa aactcctcgc
      601 gggcaagcgc tcggtgagtg aacaaaccga aaggcgcaat gagctcatta ataaagcgtc
      661 gcagtctatt caaaccgtcg tgagtggctt tgatcgcaaa aaacaggttg aagaactggg
      721 cgatttggtg caggactcgg tggcaaagac cgcgcttttt ggcgtcggcg cagctgggat
      781 tggcgtgctg gtagccacag tgattaccgc gcgcgccttg gacatcactg gcattgtttc
      841 tgccgggact ttggcggtct tgggcttatt tgtgataccc tacaaacgca aacaggccaa
      901 ggataatttt tcagaacgta tggcagaaat gcgcgcttca ttgcggagac cttaaataaa
      961 actttcaaag ccgagtttgc gcagtccgtt acccgcttac gcgagaacat ttcgccatat
     1021 actagctatg tgcacgctga aagcgcccag gcattgcaga accagtccac tttggctgag
     1081 ctgagtcaga agcttgatgc actcaataaa gaaattaatg ctaaaatctg acatacgcta
     1141 aaatatgatt gaaaaggatg tgaacaaaat gcaacgaacg aaaacaacac tcgcgaaagc
     1201 aatcttgctt ttactgatgt taagcatgat tttcgtcgct tgtgataagc aaaacggggt
     1261 tgaccctact gatggaactg agcaacaagc aacgctgagt gaaccgctgg caaatgaaac
     1321 gcccgagccc gaagcgaccg caacgcgtga tatgcgtggt gtgacccagg ttgtgttggg
     1381 gggtgatctg cccgatgacc ctgtcccgac ctggacacaa agcgctatcc taacccagga
     1441 agccctggct aatcaaacac ccaccgccac acttccagga acggtggttg ttggtgaaca
     1501 gcactatgtg gtttatcagg gtggctatgc cgtgaccctt gatttggatg ctcatgtgag
     1561 tgtgcggggt aatttgtcat taatcggctc gcgcacacat ccagtaaaca cattaattat
     1621 gggcaatgtg agcaaatacg aaattatttc agtggacgaa ctcccacaac aggtttttga
     1681 cagccttttt ggttcagagg ctcaggtaag ccaggaagaa gtcgaagaat tcgaactgga
     1741 tggtgttcag ggaatcgcgc taaactatat cgcccgcatc aatcagatga ttgccaaggg
     1801 gcgtttggtg gtggtaaaac cgactgaaaa acgctacgtg gcaattatag gtatgggtga
     1861 tccagccgat ccaagtggtg accagtggat tgccgatggg gtggaatttt ttgacgcgat
     1921 tgtgaatggt gttgaaattt tggatgatga tttactggta gacaatacgg tttgtcccat
     1981 ggcaacggat gaaagctacg gctttagccc cgataacccc atccgtgtgg gtggcgatgc
     2041 catcggcgga cccgcgcggg cacgggcata tctggacaat ctgcaggaca gcaaaggcaa
     2101 ctggctggcc tatgagcgca aaggttcaat ggagcatggt ggcgccattg tggacgccta
     2161 tgtcgtcatt ttaggtggcg tagaacgctt aatttatatt aatcaatatg actacgcaga
     2221 gctgctcaca ccagttggct ttagctgtgg tggggcatat ccgcttgtcg caccttaggc
     2281 tgaatggctt tgatacttaa ttgaaataaa accccaccgg cagaaaaagg tggggttttt
     2341 gaatccatgc ttttattgac gcataaggtg ttttgataaa aagattgaaa atccgtccta
     2401 agaaggtttg atttaagaga cgattatgga aatttgattt ttcatttatc tacacaacgc
     2461 taacactatt aagcccgact gccatgctat aattcaaagt tgggcgctat tgtttttagg
     2521 cccaacagag gacaaattat gctacaaaaa attgcacgtt tttttacggg ggaccccaac
     2581 aagaaagcta tcgacaagtt aagccttatc gtcgagcaga tcaacgcact ggagccacag
     2641 tttgaggcac ttagtgatga agccctgact gccaagacag atgaatacaa agcacgcctg
     2701 gcaaaaggcg aaacactgga cgatttactg cccgaagctt ttgccaccgt gcgcgaggcg
     2761 agcaagcgcg ttttaggcca gcgtcactat gacgtacagc ttatctgcgg tatcaacttg
     2821 catcatggtt ctatctcgga actgcgcacg ggtgagggca aaacgctgag tgccaccttg
     2881 cccctttatc tcaacgcgct tgaaggcaag ggggcgcatc tggttacggt caacgactat
     2941 ctggcacgcc gtgacggacg ctggatgggt gctatttatc gctttttggg aatgagcacc
     3001 ggcattttgc agtctgcatc agccgatggc gcttcgggac gcgcttttat ttatgacccg
     3061 gacgaatctt cactctacga agaaacccat cagttacgac cggtgcaccg caaatatgct
     3121 tatgccgcgg atatcaccta tggcaccaac agcgaatttg gctttgacta cctgcgcgac
     3181 aatatcacca tgcgctggga cgaccgcgtt cagcgaccgc atcactttgc gattatcgac
     3241 gaggttgaca acattctcat cgacgaagcg cgcacgcctt tgattatctc cggtccttcg
     3301 cacgaagatt ctgaaaacta ttaccgcatg gcccaggtcg tgaaagcact caaaccagaa
     3361 gattacgaag tgagcgaaaa ggaccactcc gttgccttga ccgaaatcgg tgtagcacat
     3421 gttgaagaat tgcttggcga gcccctttcg gaccccgaac ggcctgaaga tatcctgccg
     3481 gaacaggcac gcttgctggg ttttttggag caaagtttgc gcgcgcagtt tctcttccat
     3541 cgcaacaagg aatatatcgt gcagggtggc gaggttgtca ttgtggacga atttaccggt
     3601 cgcatgatgc ccggcagacg ctggtcggac ggcttgcatc aggcagtgga tgccaaagag
     3661 ggcgtaaaag tccaggcgga aaacatcacc cacgccacag taaccattca gaactacttc
     3721 agaatgtaca caaagctggc cggcatgacc ggtaccgcaa tgaccgaaaa agaagagttc
     3781 taccgcattt atggcctgga cgtgctggca attccatcaa accttgaata caaagccgaa
     3841 cgggctgatt cgggcatcaa gcatgcccag gcccaggatg agtttggcta tgactatacc
     3901 tattactatg ccgcggatga tcccacgcag gagccccttt attacaagcg aaaagactat
     3961 ccggacgtca tctatcgcac cggagagggc aaattgcgcg cgattgtact ggagattttg
     4021 cgttaccatg caatcgggcg tccgcaactg gtgggtacaa cttcggtgga aagttcggag
     4081 catctttcca gtcgcctgag ctccgacatg gtgcgccgtg tggtgcaggt gagccttatc
     4141 aggcgcgcct ggcgattgtc aagaggcgcc aaagaagaag actatcaaca agcgccagaa
     4201 ctggaaggac tcaacgcgcc acttgataaa ttgcgcatgc ctgaactgcg ccgtttgggc
     4261 acacagtttg gcgtgagtac gatggatatg accgacgaga gcaaccgcga aggtatcctt
     4321 gaacttttac agcttgaacc cgagcattgg gaacgcctaa aaccaacttt tgatgcgggc
     4381 attccgcatc aggtactcaa cgcgcgcaag cataccgaag agtccatcat catcgccaaa
     4441 gccggtgcgc ttggcgcggt gaccatcgcc accaacatgg ctggccgtgg tgtggacatc
     4501 aagctgggtg gtgagttgcc tgagaattta ctttcggaac tcaaccaaat cctggcaaaa
     4561 aaccgcgatt tagatcccta taacatgacc atgccccagc gtttggaggc agtgaacaag
     4621 ctagacctgg accttgagga agagcaagaa aacaccgtag acgcattcaa acagtacatg
     4681 gaacagatgg cactggtgcg cgagattggc ggtttacatg tgataggttc ggaacgccac
     4741 gagtcgcgtc gtatcgacaa ccagctacgc gggcgcgcgg gccgtcaggg tgacccgggt
     4801 tcgtcgcgct tttatctgtc attggacgat gagttgatgc gtatgtttgg cggagaccgc
     4861 atggaagggg tgctgtcacg tctcaaactg gatgaaagca tgcctatcga agtcggtctc
     4921 attaaccgca tggttgaaca agcccaaacc cgcgttgaag gcacgaactt tgatatccgc
     4981 aagcatttgc tggagtatga cgacgtgctt aacacgcaac gtaatcgggt ttatgaacag
     5041 cgcgaccgaa ttttcctgaa ggaagatttg catgatgaca ttgacgagat gattcgcaat
     5101 gaaatcgcgc cgaaagtcga actggccatg gcggacgcgg aaggaccctg gaagctttta
     5161 gcccagcttg aagatatcca gtcgatgatt aataccgaat ttggtatcta cccgtcctac
     5221 accattaaat tagtagctga tgaactggaa gatgttcagg acttgggaac gctgaaatcc
     5281 cgtatgcttg accttgccaa agacgccgta aagcaggaaa atgagcatct tcttgaaggt
     5341 gtggaactgc ttctcgcgcg caatcaggaa acgattaaga atcagattgc tgaaagaagc
     5401 gaggctttgg acgcttttct ggaaactttc gacccgaacg aaatccgcga tgtgcaacag
     5461 gaactgagca gtttgatggg cgtgcaagtg cgcctggcag ctgccgagca acgcattttg
     5521 cgcgatgacc cgatgagcat gaaacaaccc ttgcgggacg cgctacgcac gggtgtgacg
     5581 ctgaacgtca tcagacgaac gctgatgaca ctcgaaaaac gctttggcga agtctggcca
     5641 ctcaagccga ttgaccttgc aagccagccc tgggcagaaa cgcaaaacgc tatcttggac
     5701 caggcgcaag acacgctcaa ccatcgaaat acggtgcttt ttggcgacaa tggcgaagtg
     5761 ccacgcgata ttgacaacaa cgaagctctg ctaagcgagg ctttggaaga cgacgaagcc
     5821 ctttaccgcg cgatacaaat gaccatgatg ggcaagcgcg ttggctttga cgcgcggtca
     5881 cgccagcgcc aaatccggac gcacatgcgt ttgaattaca ttttctcgat ggctgaaaaa
     5941 atcaggaaga tgaacgtgga agcgctcacg gaagatattc tcacccatct tgatagcgct
     6001 gaagagcatc ttgcagaaat ctttggcatg gcggactgga tgaatgtcat gcacaaaaac
     6061 cgcgtg
//
LOCUS       DAOSFC010000058         6036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_11540_length_6036_cov_5.652566, whole genome shotgun sequence.
ACCESSION   DAOSFC010000058 DAOSFC010000000
VERSION     DAOSFC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11540_length_6036_cov_5.652566"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK791_02460"
     CDS             complement(<1..282)
                     /locus_tag="PK791_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92208.1"
                     /translation="MTDEKLRILKMLENGTITATQATDLLAALQEEPITESPITNDGP
                     VTRAQWMKILIIDRLSGEKKVNLRFPLALLPWNKMEKGLNINLGNKTDEN"
     gene            complement(284..682)
                     /locus_tag="PK791_02465"
     CDS             complement(284..682)
                     /locus_tag="PK791_02465"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOH92209.1"
                     /translation="MEFTNTCPICQSKVRITRYHCDSCQTTIEGSFVLPVDPFSRLSE
                     DQRHFMLTFVRAEGRLNRMEEILGMSYPTLKNRLNELITTLGFQAELYSKKPNAAERQ
                     AILDDLEAGRIDSDQALRYLQGDEPFITGE"
     gene            920..2446
                     /gene="ftcD"
                     /locus_tag="PK791_02470"
     CDS             920..2446
                     /gene="ftcD"
                     /locus_tag="PK791_02470"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOH92210.1"
                     /translation="MKIVECIPNFSEGRRPEVIKAIIDAVAAVDGVRILDHSSDVDHN
                     RTVLTFVGDPKAVEEGAYACIAKAAELIDLNTHHGEHPRMGATDVVPFVPISDVTMAD
                     CIEMARRLGKRVAGELNIPVYFYEDAAIRKDRVNLEDIRRGEYEGIKETIATDPYKEP
                     DLGPKELGPAGATVIGARQPLIAYNIFLNTDDVSIAQKIARRTRHSNGGFRFVKGIGL
                     LVDGLAQVSMNLTDYRRSPLAQVTEFVRREAQRYGVGIHHTEIIGLVPQRAMINACKW
                     YMQIDGLQDEQILENQVFGSQSSDSAEENIYGFIDEVAAGTPAPGGGSSAAYAGALGA
                     ALTLMNARLTVGKKRYADVENEVFEVIEQAEPLRQALTEGIEKDAKSYDGVVEAMRLP
                     KGTEEEIAARDEAILKASLVAAQVPLQTARDCLAALKLAKRMCEIGNENAISDAAAGA
                     HMAKAGFMAAGLNVRINLLGYDDNKDAQALKAEWDDMAKEVNALMDAMTAIVHERMGI
                     "
     gene            2451..2894
                     /locus_tag="PK791_02475"
     CDS             2451..2894
                     /locus_tag="PK791_02475"
                     /inference="COORDINATES: protein motif:HMM:NF003501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HOH92211.1"
                     /translation="MTQSKFWLEKAIEDLTPSEWESLCDFCSKCCLHKFIEPGKTEVQ
                     FTNVVCRYMDMETCKCSDYENRHDNVPDCVLLTPKLARGANWLPKNCAYRLVADGKDI
                     PWWHPLKTGDPDSTRKTGNSVFGKVVSEDDIDPEDLEDMVVDWFN"
     gene            2942..3283
                     /locus_tag="PK791_02480"
     CDS             2942..3283
                     /locus_tag="PK791_02480"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOH92212.1"
                     /translation="MSDQKKPDKLIIAVVQSQDGELVERTLEEANYSFGKLPSVGGFL
                     RERNETFLIASSEAEIPELLDLLSSVAKRRVSYLATPIDNAPFPIVIPTETVIGGANV
                     FELDLDYFEEF"
     gene            3285..3551
                     /locus_tag="PK791_02485"
     CDS             3285..3551
                     /locus_tag="PK791_02485"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOH92213.1"
                     /translation="MKLIIAIIQDADNDRVSAALTDEKYRVTFIASTGGFLRSGRSTL
                     LIGTEEDRVARALEIIRETSTEPKNENEKRATVFVVDVAKFTQL"
     gene            3573..4262
                     /locus_tag="PK791_02490"
     CDS             3573..4262
                     /locus_tag="PK791_02490"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HOH92214.1"
                     /translation="MNFGKSFTYIFEDPQWFDKLWKPVLCMLIPVIGPLVFQGYIMDV
                     IKNVANGDPRPLHEMDFGENLTMGFRNFVVQLVYSLPLIVLSWLIVIPSMLSVGAMAD
                     EKLSLVMAVPALLSQLGLSLFSMVLSAVFYLVFPIICAHVAVKGTIKSGFEFREIFNL
                     AKKNFAAWLMVLAGNLIAGFIGPIGVIVFFAGMFVTMAYSMLMVAHLSGQAYRASQPT
                     VVYPSYPNYPG"
     gene            complement(4368..4937)
                     /gene="amrA"
                     /locus_tag="PK791_02495"
     CDS             complement(4368..4937)
                     /gene="amrA"
                     /locus_tag="PK791_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOH92215.1"
                     /translation="MNNKLKPEEKKTLLKIARDAITAHVNGQKPKQLDEYPLSPALLT
                     DGASFVTLTKKGKLRGCIGTLQAVQSLAEDVQLRAIQAATEDPRFPAVQASELAQIKI
                     EISRLTPPVKLDYENPQDLAQLIRPHQDGVILKDGFRRATFLPQVWDQLPYPEDFLSH
                     LCQKMGVPGGYWKKKLLEVSIYSVEDFSE"
     gene            5075..6013
                     /locus_tag="PK791_02500"
     CDS             5075..6013
                     /locus_tag="PK791_02500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH92216.1"
                     /translation="MNNEPTNVDDFPEDEQPAYDASDAIADDEDLSENEENFEDTLSN
                     TSSQTIRGLVTSPRPAQSSRAAVAYRPRVTLQTLATVIFAAVLVATLLTLWMPSAFTG
                     DNLQEQYVAEISVPQITQQAEEGLEVSLPQEFPENKIGIIIGHKGRDEGEVCSNGLTE
                     VEINSTVATYLQQKLIELGYDAELLDETDPRLVGYQAQLLIALHCNSCEYINDNATGF
                     KFAIVPSEVKTIDRELLTKCLSEAYANATGLKYHYQTTTNEIRNYHAFDQIDPLTAAM
                     IFELGYMNLDQDILTNRPEVLAEGLANGIQCYMNAD"
BASE COUNT         1561 a         1278 c         1562 g         1635 t
ORIGIN      
        1 gttctcgtcg gttttgttcc cgagattgat gttgaggcct ttttccattt tattccacgg
       61 taacaatgct agcgggaaac gtaaattcac tttcttttct ccgcttaagc ggtcaatgat
      121 aagaattttc atccattgtg cccgagttac agggccatca tttgtaatgg gtgattcggt
      181 gatgggttct tcttgcagtg cggcaagtaa atcagtggct tgggtggcgg tgatggtgcc
      241 gttttcaagc atttttaaaa tgcgcagttt ttcgtcggtc attttattct cctgtaataa
      301 aaggttcatc gccttgcaga tagcgcaggg cctggtctga gtcaatacgt ccggcttcaa
      361 ggtcatccag gatagcctgg cgttcagcag cgtttggctt tttgctgtag agttctgcct
      421 ggaagcccag ggtggtaatc aactcattca aacggttttt aagggttggg taggacatac
      481 ctaaaatttc ttccatgcgg ttaaggcgtc cctcagcacg cacaaaagtt agcataaagt
      541 gacgttggtc ttcgctcaag cgactaaatg ggtcaacggg aagcacgaaa gaaccttcaa
      601 tcgttgtttg gcagctgtcg cagtggtagc gagtgatacg tactttgctt tggcaaatgg
      661 ggcatgtgtt ggtaaattcc atcgttttta gtgtcctttc agtaatttag ctagcttcaa
      721 tgcttattct acatagaaac ttaattaagt caagttaaaa atgcaagaaa cttaatggaa
      781 ttcgcaaaaa gcctaaagaa aatcaataac agattgaaaa taactgaaaa tgtagattaa
      841 tttcttccct gcgtgcattt gggaaatttg gtataattta aagttgctaa taaaacagta
      901 aaaggagtgt gtcaaatcca tgaaaatcgt tgaatgtatc cctaatttct cagaaggtcg
      961 tcggccagaa gtcattaaag ccattatcga cgcggttgcc gcggtggatg gtgtgcgcat
     1021 tttggaccac agttctgatg ttgaccataa ccgaactgta ctcacctttg tgggtgaccc
     1081 aaaggctgtt gaagaaggcg cttatgcttg tatcgcaaaa gcagctgaac tcatcgacct
     1141 gaacacgcat cacggtgaac acccgcgtat gggcgcgaca gatgtggtgc cttttgttcc
     1201 catttcggac gtgaccatgg cggattgtat cgaaatggcg cgccgtttgg gtaagcgtgt
     1261 agctggggaa ttgaacattc cagtttattt ttacgaagac gcggctattc gcaaagaccg
     1321 tgtgaacctg gaagatattc gccgcggtga atatgaaggc attaaagaaa caatcgccac
     1381 ggacccttac aaagaacccg acctgggacc caaagaactg ggcccggctg gcgcaacggt
     1441 gattggcgcg cgccaaccct tgattgccta taacattttc ctgaatacgg acgatgtcag
     1501 tatcgcgcag aaaattgcac gccgaacgcg ccattccaac ggtggtttcc gctttgtgaa
     1561 gggtatcgga cttttggttg atggtctggc acaagtttca atgaatctca ccgactatcg
     1621 tcgttcaccc ctggcacaag tgaccgagtt tgtgcgccgt gaagcccagc gttatggtgt
     1681 gggtattcat cataccgaaa tcatcggatt ggtgccccag cgcgcgatga taaacgcttg
     1741 caagtggtac atgcaaattg atggtttgca ggatgagcaa attttggaaa accaggtctt
     1801 tggttcgcag agttccgatt ccgctgaaga aaacatttat ggttttatcg acgaagtggc
     1861 cgcgggaaca cctgctcctg gtggtggctc atctgcggct tacgcgggtg ccttgggtgc
     1921 cgcgctgacc ttgatgaacg cacgccttac cgttggcaag aaacgctatg cggacgttga
     1981 gaatgaagtc tttgaagtga tcgaacaggc tgaacctttg cgccaggcgc ttaccgaagg
     2041 cattgagaag gatgccaagt cctatgacgg tgtcgttgaa gcgatgcgtt tgcccaaggg
     2101 aactgaagaa gaaattgccg cgcgtgatga agcgatattg aaagccagtt tggtggccgc
     2161 gcaagtgccc ttgcaaaccg cccgtgattg tttagccgca ttgaaacttg ccaagcgcat
     2221 gtgcgaaatt ggcaacgaaa acgcgatttc cgacgcggct gccggcgcgc atatggcaaa
     2281 agctggtttt atggccgctg gtttgaatgt gcgcattaac ctgctgggct atgacgacaa
     2341 taaagacgcc caggctctga aagcggaatg ggacgacatg gcaaaagaag ttaatgccct
     2401 gatggacgcc atgaccgcta tagtgcacga acgtatggga atctaagaat atgacccagt
     2461 cgaagttttg gcttgaaaaa gcaatcgaag atttaacgcc gtcggaatgg gaatccttgt
     2521 gtgatttttg ctccaagtgt tgcttgcata agttcatcga accaggcaag acggaagtgc
     2581 agtttacaaa tgtggtttgt cgctacatgg atatggaaac ctgtaaatgc agtgattacg
     2641 aaaaccgaca tgataacgtg cccgattgcg ttttgctgac gccaaaactg gcaaggggtg
     2701 ccaactggtt gcctaaaaat tgcgcctacc gtttggtagc ggatggcaag gacattccct
     2761 ggtggcatcc cttaaagacg ggtgaccctg acagtacccg aaaaacaggc aattctgttt
     2821 ttgggaaggt ggtcagtgag gatgatattg accctgaaga tctcgaagat atggtcgtgg
     2881 attggtttaa ttaaaaacac aaaaagcgtg taaaataaag atatcatccg aaggaggcag
     2941 aatgagcgat caaaagaaac cagacaagct gataatcgcg gtcgtgcaaa gccaggatgg
     3001 cgaactggtt gaacgcacgc tggaagaagc aaactatagc tttggcaaac tacccagcgt
     3061 gggtggtttt ttgcgcgaaa gaaatgaaac ctttctgatt gcatcttcag aggcggaaat
     3121 tccagaattg ctggaccttc tgagttctgt agctaaacga cgggtgagtt atctcgccac
     3181 acctatcgac aacgcgccat ttccgattgt tattccaact gaaacggtta ttggtggcgc
     3241 gaatgttttt gaattagatt tagactattt cgaggagttc taagatgaaa ctcatcattg
     3301 caatcattca agatgctgat aacgaccgtg tatctgccgc gttaactgat gaaaaatatc
     3361 gcgtgacctt tattgcttcc acaggtggtt ttttgcgcag tggcagaagt accttgttaa
     3421 tcggaacgga agaagaccgt gtcgcgcgcg cgcttgaaat cattcgcgaa acaagcaccg
     3481 agcccaaaaa tgagaacgaa aaacgcgcga cagtttttgt ggtcgatgtg gcgaagttta
     3541 cgcaacttta aacacccaga tttgaggaaa taatgaattt tggtaagagt tttacgtata
     3601 tttttgaaga cccgcagtgg tttgacaaac tttggaaacc cgtgctttgt atgctgattc
     3661 cagttattgg accccttgtt tttcaggggt atatcatgga cgtgataaaa aatgtggcaa
     3721 atggcgaccc acgccccttg cacgaaatgg attttggcga aaacttaacg atgggttttc
     3781 gtaatttcgt ggtgcagttg gtctattctt tgcccttgat tgtgttgtct tggttgattg
     3841 tcattccgag catgctgtcg gttggtgcca tggcggatga aaaactttcg cttgttatgg
     3901 ctgtgccagc gctattgtct caactcgggc tttcattgtt ctcaatggtg ctaagtgctg
     3961 tgttttattt ggtcttcccg atcatttgcg cgcatgtggc cgtgaaaggg accattaaat
     4021 ctggttttga gttcagagaa atcttcaatt tagccaagaa aaatttcgct gcctggctga
     4081 tggtattggc aggaaacctg attgcgggct ttattggacc gataggcgtg attgtctttt
     4141 tcgcaggtat gtttgtaacg atggcttatt caatgttgat ggtagcccat cttagcggac
     4201 aggcctatcg cgcgtcccaa cccacggtgg tttatccaag ttatcccaac tatcccggat
     4261 aatcgcaaat agcgcttaaa attaaaactc acgcatattt atcagcgtga gtttttgcta
     4321 ttaagattac gaagcatcag gttaatgggc gtggactact ccgaaaacta ttctgaaaaa
     4381 tcttcaacgc tgtaaatgga aacttccagg agttttttct tccagtatcc accaggcacg
     4441 cccatttttt ggcataaatg gcttagaaag tcttcaggat agggcagttg gtcccaaacc
     4501 tgtggcaaaa aggtcgcgcg acgaaaacca tctttcagga taacgccgtc ttgatggggt
     4561 ctaatcagct gagccaggtc ttgcgggttt tcatagtcaa gctttacagg cggggtcagg
     4621 cgggaaatct caattttgat ttgcgccagt tccgaagctt gcacagctgg gaaacggggg
     4681 tcttctgtgg ccgcctgaat cgcgcgcaat tgcacatctt ctgccagact ttgcacggct
     4741 tgcagggtgc ctatgcatcc ccgcagcttg ccttttttag tgagagtcac gaaactggca
     4801 ccatctgtca aaagcgcggg gcttaatggg tattcgtcca gttgttttgg cttttgcccg
     4861 ttgacgtggg cagtaatggc atcgcgggca atctttaaca aggtcttttt ctcttcgggc
     4921 tttagtttgt tattcatagg gactcctttg tgtttattat acaggccttt gcctgtggac
     4981 gggggcaaaa attttggttt attaagctgg gataaacaaa acctaactgc cttaaataat
     5041 tgttcaggcg tgagaattaa ggtaagatta aggcatgaat aatgagccaa cgaatgttga
     5101 tgatttcccc gaggatgagc agccagcgta tgatgcatcc gacgcgatag cggacgatga
     5161 ggacttgtcc gaaaatgaag aaaattttga agacacgcta agtaatacca gctcacagac
     5221 gattaggggg ctggtaacca gtccgcgtcc agcgcaaagt tcgcgtgccg cagtcgcgta
     5281 ccgaccgcgg gtaactttgc agaccctggc gacagtcatt ttcgcggcgg ttcttgtggc
     5341 aaccttgctt actctgtgga tgccaagtgc ctttacgggt gataacctgc aggagcaata
     5401 cgttgcggaa atatcggtgc cccaaataac gcaacaagct gaagaagggc ttgaagtgtc
     5461 cttgccacag gagtttccgg agaataaaat cggcattatt atcgggcaca aggggcgcga
     5521 tgaaggcgaa gtttgttcga atggcttgac cgaagttgaa attaacagca ccgtcgccac
     5581 ctatttgcag caaaaactga ttgaactggg ctacgacgct gaattgcttg atgaaactga
     5641 tccgcgtttg gttggttacc aggcccagtt gctcatcgcc ttgcattgta attcctgtga
     5701 atatatcaac gacaatgcca ctggctttaa gttcgcgatt gtgccttcgg aagtcaaaac
     5761 cattgaccgc gaattgctta ccaaatgcct gtcggaagcg tatgcaaacg cgacgggctt
     5821 aaagtatcat taccaaacca cgacgaacga gattcgcaat tatcacgcgt ttgaccaaat
     5881 tgaccctttg accgcggcga tgatttttga actgggttat atgaatcttg accaggatat
     5941 tttgactaat cgaccagaag ttttggcgga gggtcttgct aatggtattc agtgttatat
     6001 gaatgcggac tagcttatga ataatatgga accatc
//
LOCUS       DAOSFC010000059         6014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_11611_length_6014_cov_4.724283, whole genome shotgun sequence.
ACCESSION   DAOSFC010000059 DAOSFC010000000
VERSION     DAOSFC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11611_length_6014_cov_4.724283"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..2639)
                     /locus_tag="PK791_02505"
     CDS             complement(63..2639)
                     /locus_tag="PK791_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOH92217.1"
                     /translation="MSTELFPERSKSKPVIYAYTDNNPAYAGMLKIGYTTRTAEERVA
                     EQYPVVRPGTKPYTIVFEEPAMRNDGTGFNDYDVFAALHRMQMQHEAGEWFSISVEQL
                     KAAYISVRDRKENLEMRSLDFSMRPEQEAAVRKTMAYFQSVWEDSSTSASPKFLWNAK
                     MRFGKTFAAYQLAKKMEMKKILVLTFKPAVESAWEEDLLHHVDFEGWQFISRTTELTY
                     EKADLSRPIVCFGSFQDYLGTEAGGRIKAKNEWVHTTNWDLVIFDEYHFGAWRDNARK
                     LFADQDEDDVDFDSEAYQKDEAGNAMDESFLPITSYHYLFLSGTPFRALGSGEFIEEQ
                     IFSWTYSDEQSAKEDWGNRPDNPYASLPQMVLMTYRLPAEITRVAEEGEFNEFDLNVF
                     FSAEGEYENARFVYEDYVQKWLDLIRGAYMPAATDDLKLGWGKPALPYSDSRLLSVLT
                     HTLWFMPNVSACYAMANLLKQRQNIFYHDYTVNVAAGTRAGVGLAAVEPVKKSMANPL
                     ESKTITLSCGKLTTGVTIRPWTGIFMLRNLKSPETYFQAAFRVQSPWVIKGEDGKSEI
                     IKEVCYVFDFAIDRALKQIADYSVQLNVEESNPEVKVGEFIRFLPILAYDGSTMKRID
                     AAGVLDMALSGTTATLLARRWESALLVNVDNLTLGRLMANQEAMDALMRIEGFRSLNQ
                     DIETIINKSEAVKKAKKEQGDTLSKAQRKELSEEEKEFKSLRKQIQEKLIKLATRLPI
                     FMYLTDYREHSLKEIIRNVETGLFRRVTGLGVNDFDLLVSLGVFNGPLMNDAIWKFKR
                     YEDSSLTYAGVNRHEGERVGAWDTSVSYEDYLRVVQSRSPRLWESYGVYDVSPASADE
                     QS"
     gene            complement(2641..4173)
                     /locus_tag="PK791_02510"
     CDS             complement(2641..4173)
                     /locus_tag="PK791_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOH92218.1"
                     /translation="MLKTNYQTLHSPDVLDCLANLSSDEIFTPPKVARQMLDLLPEEL
                     WSDPEAKFLDPATKSGVFLREIAKRLLKGLESQIPDLQERIDHIYQKQLYGVAITELT
                     DLLTKRSLYCTKYPSGRYSVSQFPKERANGNIYYKNIRHVWKDGRCQYCGASQNEYDR
                     DPELESHAYAFIHRDNPEELFNMRFDVIIGNPPYHLSTGGAGRQAKPIYPLFIEQAIK
                     LNPRYLSMIIPSRWFAGGMGLDGFRDKMLNDKSISVLVDFINAKDCFPQSSISGGVCY
                     FLREREHDGLCKFVSNRGGKTFEMVRNLSEHGVFIRYNEAVEIIRKIKSIGEPPLSEL
                     ASPLFPFGLSTDYRGVSTEKDGDLKLHSSEGVSYISRSEVSSGLDLIDVYKVLVSKTS
                     SEHAGEPGSDGRYRVLTSSIKVLEPGEVCTHSYFVIGSFSNKLEAKNLLNYLTTKFVR
                     LLTLISMSSINLSRGVFQYVPQQSWGKIWTDEELYAKYELTEDEIDFIESMIKPMDLE
                     ES"
     gene            complement(4180..4965)
                     /locus_tag="PK791_02515"
     CDS             complement(4180..4965)
                     /locus_tag="PK791_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92219.1"
                     /translation="MDRLCENYPDMYLGLLAAKKEFDTVNPSFKPKIIYAQKLFFERN
                     NIYDSEYIQRCDELLELVLYRDAAAYFTLEFFHSIRNTKDYCSAYIADIKIDRDNDMF
                     LHQVLLDQYLLLAYASLEYYLNYIYYFCLRKEPKHKGLRKILNELERSESERVIMTYQ
                     YIVTQICNPFDEKSTLWGDKLRDLRNRTAHEKNVQIGTIQRENLLKITREEPALEGQQ
                     IAHYLEHQFVAKKVLMFEKMNLLLYGIPWVPGPFHERIYDEKL"
     gene            complement(4958..5623)
                     /locus_tag="PK791_02520"
     CDS             complement(4958..5623)
                     /locus_tag="PK791_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HOH92220.1"
                     /translation="MENGIDQVKSKQRVADHGEVFTAEREVNAMLDLVKHETERIESR
                     FFEPACGTGNFLAEILRRKLAVVKARYGKHPADFERYAFLALSSIYGVELLEDNAKEC
                     RARLFGILDQAYTRVCKKDASDDFRQVARYVLERNILCGDALTLKDAKGEPIAFSEWS
                     LVTGNLVQRRDFHLSQLLDASEQQMTIFMDGWEYDADLGQYIPQPFREFPLMDYREVQ
                     NYG"
     gene            complement(5815..>6014)
                     /locus_tag="PK791_02525"
     CDS             complement(5815..>6014)
                     /locus_tag="PK791_02525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOH92221.1"
                     /translation="ALSAVIFGGVHYFGTPGKIPGVIMAAFIGWFLSKSIHETKGFFW
                     AWLIHFLQDVVIMTALFLTSV"
BASE COUNT         1707 a         1471 c         1140 g         1696 t
ORIGIN      
        1 tagcatgatt atgcctggct acacaaaaat atttccgtaa tgtaaattat gggtattgct
       61 atttaacttt gctcatcagc gcttgcaggc gacacgtcat agacaccgta tgattcccac
      121 aaacgcggac tgcgactctg cacaactcgc aagtaatcct cataactcac gctcgtgtcc
      181 catgcgccca cgcgttcacc ttcatgtcga ttaacgccgg cataagtcag gctggaatcc
      241 tcataacgct taaacttcca aatagcatca ttcatcaacg gaccattaaa cacacccaac
      301 gacaccaaca aatcaaaatc attcacgccc aagcccgtaa cacgtctaaa caagcctgtt
      361 tcgacattgc ggatgatttc ttttaaacta tgttcccggt agtcggtcag atacataaaa
      421 atgggtaagc gtgtggctaa cttgattaac ttctcttgaa tttgcttgcg caaactctta
      481 aattcctttt cttcctctga aagctcctta cgttgcgctt tgctgagtgt gtctccctgt
      541 tcttttttag ccttctttac cgcttcagat ttgttaataa tcgtctcaat atcctgatta
      601 aggctcctaa aaccttcaat gcgcatcaag gcatccatcg cctcctgatt agccattagc
      661 ctgcctagag ttaaattatc cacgttcacc aaaagcgcgc tttcccaacg cctggccagc
      721 aaggttgccg ttgtacctga aagcgccata tccagcaccc cagcagcatc aatgcgtttc
      781 atcgtactgc cgtcatacgc caaaatgggc aaaaagcgaa taaactcccc aactttcact
      841 tccggattgc tctcttcaac attcaactgc acgctgtaat ccgcaatctg tttcaaagcg
      901 cggtcaatgg caaagtcaaa aacataacaa acttctttaa taatctcgct tttaccatct
      961 tcacctttaa tcacccacgg actttgtaca cgaaaagcgg cctgaaaata agtttccggg
     1021 cttttcagat tccgcaacat aaagatgccc gtccaaggcc ttatcgtcac gccagtggtc
     1081 agcttgccac aactcagtgt aatggttttg ctttccagcg gattagccat tgacttctta
     1141 accggttcaa ccgctgccag acctaccccc gcgcgggtac cagctgccac attcacagtg
     1201 taatcatgat aaaagatgtt ctggcgttgt ttcagcaaat tagccatcgc gtagcacgcg
     1261 ctcacattcg gcataaacca caaagtgtgt gtaagaacgc tcaacaaacg actgtccgaa
     1321 tacggcaagg ctggcttgcc ccaaccaagt ttcaggtcat ccgtcgcggc tggcatataa
     1381 gcaccccgaa tcaagtccag ccacttttga acgtaatcct catacacaaa gcgcgcgttt
     1441 tcatactcac cctcagcaga aaagaaaacg ttcaggtcaa actcattaaa ctcaccttct
     1501 tcagcaacac gtgtaatttc agcgggcaaa cgataagtca tcagcaccat ttgcggcaaa
     1561 ctggcataag ggttgtcagg acgattgccc cagtcctctt tcgcgctttg ttcgtccgaa
     1621 taggtccagc taaaaatctg ttcttcaata aactcacccg aacccaaagc cctaaagggt
     1681 gtaccggata gaaacagata gtggtaactg gttatcggca aaaaactttc gtccatcgcg
     1741 ttgccggctt cgtctttttg gtaagcttcg ctgtcaaaat cgacatcatc ttcatcctgg
     1801 tccgcgaaaa gtttgcgggc gttatcgcgc caagcaccaa agtggtactc gtcaaaaatg
     1861 accaaatccc agttggtggt gtgcacccat tcatttttgg ctttgattct tccacctgct
     1921 tcagtcccta aataatcctg aaacgaacca aaacacacaa tcggacggct taaatcagcc
     1981 ttctcatatg tgagttcagt cgttcggctg ataaactgcc agccctcaaa gtcaacatgg
     2041 tgtaacaaat cttcttccca ggcgctttcc accgcaggtt taaaggttaa gaccaaaatc
     2101 tttttcatct ccattttttt ggccaactga taagccgcga aggttttacc aaagcgcatt
     2161 ttggcgttcc aaagaaattt tggcgacgca ctggttgatg aatcttccca gacggactga
     2221 aagtacgcca ttgttttacg cacagcggct tcctgttcag ggcgcattga aaaatcaagg
     2281 ctgcgcattt ccaggttttc tttacggtcc cgcacactga tataagccgc cttgagttgc
     2341 tccacactga tgctgaacca ttcacctgct tcgtgttgca tttgcatgcg gtgtaaagca
     2401 gcaaaaacat cataatcatt aaaacccgtg ccatcattac gcatggcagg ctcttcaaaa
     2461 acgatggtgt aaggttttgt tccgggacgc accacaggat actgttccgc tacacgttcc
     2521 tctgcagtcc gggtggtgta accaatcttt agcatacccg cataagcggg attgttatcc
     2581 gtgtaggcat aaataaccgg ttttgatttg gatcgctcgg gaaataattc ggtgctcatt
     2641 ttaactttct tccagatcca tgggtttaat cattgattca atgaaatcaa tttcatcttc
     2701 tgtcaactca tatttggcat aaagttcttc atctgtccat atttttcccc atgattgttg
     2761 aggcacatat tgaaaaacac ctcttgataa attaattgat gacattgaaa tcaaggtcaa
     2821 caagcgaaca aactttgtgg ttagataatt aagcaagttt tttgcttcca acttgtttga
     2881 gaaagaacca ataacaaaat aagaatgtgt gcatacttca ccaggctcaa gtacttttat
     2941 tgaagaagtc aatactcgat acctgccatc ggaacctggt tcgccagcat gctcggagct
     3001 cgttttactc actaaaactt tgtacacatc tattaaatcc aaaccgctag aaacttcaga
     3061 tctagaaata tatgagacac cttcgcttga atgcaacttt aaatcgccat ctttctctgt
     3121 tgaaacacct cggtaatcag tagataaacc gaacggaaat agagggcttg ctaactctga
     3181 caatggtggt tctccaattg atttaatttt cctaattatt tcgacagctt cattgtagcg
     3241 aataaaaacc ccatgctcgc tgagatttct taccatttca aatgtcttac cgcctcgatt
     3301 agaaacaaat ttgcagaggc catcatgctc tctctctctt agaaagtagc agacacctcc
     3361 actaatactc gattgaggaa agcaatcttt agcattaata aaatctacta gaactgatat
     3421 gcttttatca ttaagcattt tatctctaaa gccatccagt cccatacctc ccgcaaacca
     3481 gcgtgatgga ataatcatcg agagatatcg aggattcaat tttatagctt gttcaataaa
     3541 caatggataa atcggcttag cttgacgacc agcaccacct gtactcaagt gatatggtgg
     3601 atttccaatg ataacgtcaa atctcatatt aaataactcc tcaggattat cgcggtgaat
     3661 aaaggcataa gcgtggcttt ccagttccgg gtcgcggtca tattcattct gagatgcgcc
     3721 acaatactga cagcgcccat ctttccagac atggcgaata ttcttataat aaatattgcc
     3781 attcgcccgc tctttgggga actgactcac cgaataccgc ccactgggat acttggtgca
     3841 atacaaactg cgcttggtga gtaaatcagt aagttcagtg atggcaacac cataaagctg
     3901 tttttgataa atgtggtcaa tccgctcttg caaatcggga atttggcttt caagcccttt
     3961 tagcagacgt ttggcaattt cgcgcagaaa aacgccgctt ttcgtggctg ggtccagaaa
     4021 ttttgcctcg gggtcgctcc atagctcctc tggcagtaaa tccagcattt gcctggcaac
     4081 tttaggcggg gtaaaaattt catcactgct caggttcgcc aaacaatcta gtacatcggg
     4141 actgtgaaga gtttgatagt tcgtctttag catctcatct tatagttttt catcataaat
     4201 tctttcatgg aaaggtccag gaacccaagg gattccataa agtaagaggt tcattttttc
     4261 aaacattaat acttttttag caacaaattg atgttcaaga tagtgtgcaa tttgctgacc
     4321 ttccaaggca ggctcttctc tagttatctt taacaagttt tccctttgaa ttgtacctat
     4381 ctgaacattt ttttcatgtg cggttcggtt tctaagatcc ctcagcttat cgccccaaag
     4441 agtagatttt tcatcaaaag gattacatat ttgggtaaca atatactgat atgtcattat
     4501 tacccgttcg ctttctgaac gttctaattc atttaggatt ttacgaagcc ccttatgctt
     4561 aggctctttt cgtagacaaa aataataaat gtaattgaga taatattcta atgatgcata
     4621 tgcaagaagt aaatactggt ccaatagtac ctgatgaaga aacatatcat tatctctatc
     4681 aatttttata tcagcaatgt aagcagaaca ataatccttt gtgtttctta ttgaatgaaa
     4741 aaactcaagt gtaaaatagg cagctgcatc ccgatataag acaagctcca ataattcatc
     4801 gcatcgttga atatactcag aatcgtaaat gttatttcgt tcaaagaata gcttttgagc
     4861 atagattatt ttaggcttaa aagacgggtt aacagtatca aattctttct ttgcagccaa
     4921 aagacccaaa tacatatcgg gatagttctc acacaatcta tccataattt tgcacctcgc
     4981 ggtaatccat cagcggaaat tcacggaaag gctgcggaat gtactgtccc aggtcggcat
     5041 catattccca gccatccata aagatcgtca tttgctgttc gctggcatcc aaaagctggc
     5101 tgagatgaaa atcgcgccgt tgaaccaaat tacccgtcac cagggaccat tccgaaaaag
     5161 caatcggctc acctttggca tctttgagtg tcagcgcgtc gccacataaa atgttccgtt
     5221 ccaggacata gcgtgccacc tgtcgaaaat catctgaggc atccttttta caaaccctgg
     5281 tataggcttg gtctaaaatg ccaaaaaggc gcgcgcgaca ttctttggcg ttgtcttcaa
     5341 gcaattccac gccataaata ctgctaaggg cgagaaaggc gtaacgctca aaatcagctg
     5401 ggtgtttgcc atagcgggct ttgaccacag caagtttccg gcgcaaaatt tctgccaaga
     5461 aattacccgt gccacaagcc ggctcaaaaa aacgactttc aatgcgctcg gtttcgtgtt
     5521 tgacgaggtc cagcatggca ttaacttccc gctcggcagt aaagacttcg ccatgatcgg
     5581 cgactcgttg ttttgattta acctggtcga tgccgttttc cattcgcctc cgatgctttc
     5641 gtttttataa ttataccctg tgctaaggca actttcgcaa aacaaaaaac ttggttccat
     5701 tcgcggtgcg ttataggatt aatattttca tcacccctgt agcataaaaa ttgaactccg
     5761 ggcaggtttt gcccagagtt cagtaaaaga gattagccag ttggcccgaa gggattagac
     5821 tgatgtcagg aacaaagctg tcataatcac cacatcttgc aggaagtgaa tcagccaggc
     5881 ccaaaagaaa cccttggttt cgtgaatact tttcgacaaa aaccagccga taaatgcggc
     5941 cataatcaca cccggtatct tcccgggtgt gccaaaataa tgaacaccac caaaaataac
     6001 cgccgataac gctt
//
LOCUS       DAOSFC010000060         5921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_11849_length_5921_cov_4.762700, whole genome shotgun sequence.
ACCESSION   DAOSFC010000060 DAOSFC010000000
VERSION     DAOSFC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11849_length_5921_cov_4.762700"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PK791_02530"
     CDS             complement(<1..279)
                     /locus_tag="PK791_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative C-S lyase"
                     /protein_id="HOH92222.1"
                     /translation="MNFDKNIDRRQTESLKWRRWDPDILAMWVADTDFEVPQEVMDAI
                     HARVDHGILGYGFAPDGLTDVVTSRLARLYDWQVSNEQVQYVPGIVTGF"
     gene            433..1272
                     /locus_tag="PK791_02535"
     CDS             433..1272
                     /locus_tag="PK791_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92223.1"
                     /translation="MKLLRLKTGLAVTLFASLLGVAASQVTPNWKMLCIILANIFSSW
                     ILFLFRDWQYAPQDISIQADDMQNPIAAGIIKLNTAKLLLLVLVGITITLYAIPGTKT
                     LITGLALLAIIFILNWRKLGLQKIFHLKTSPYNWLVNAFFCLLGSLGLADELNQFSML
                     AIAFVVLIMLYISLIDQARQGKSARTLRRLNIISAICLSLASIIGIMLFIIFELLPYW
                     VTGLSLILMAIMVVPQTLERFRAKEKPLFENDLLRVAFFRSGALGLMVHFLVPLIYSL
                     FIK"
     gene            1279..2163
                     /locus_tag="PK791_02540"
     CDS             1279..2163
                     /locus_tag="PK791_02540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92224.1"
                     /translation="MKQNIQPHYFETATAWHEHELLDSGDGKILERFGKMVLVRPYAQ
                     AFWPKSTPELWQENHARFQADLHNFGGKWLYKDNAQKSWPLRWEMLRFQTQLGASQHI
                     GVFPEEIKHWLWLQDTIKKAQNPVKVLHLWAYTGLMNLVATHAGADVTHVDSSKHGIG
                     WAMANQKLSSRKDAPIRYIQEEPLKFIKREARREKNYNALILQFPRMENEEESRYTDF
                     LKQLPDLILAIKDILDPNPQFILITAPACIHPVTTQAIVDDLKPGRGTISSGVICLKE
                     KSAGHLLPHASFTRWSSN"
     gene            complement(2160..3497)
                     /locus_tag="PK791_02545"
     CDS             complement(2160..3497)
                     /locus_tag="PK791_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOH92225.1"
                     /translation="MPIIFNIIFIFILFGLNAVFAMYEIAMVSARKVRLEQRVAEGKK
                     GAAYALELLKDPNQEYLSAIQIMITMIDTLAGGIGGAVISQPLAEIMKQVTWLAPIAE
                     TFALLIVVILITYFSIVLGELIPKRIAVSEPENVVTALSPMVRTLTRILKPLTRLLSG
                     STNIGLRVLRIELSPEPSITEEDVKGFIEEGRQTGVFDQAEQEMVDGVFRLTERRIDA
                     LMTPHTELDWINIEDEQEEIIHDIKASPYSRMPVAEGDLDRTIGMLHIKDLVGVDIYA
                     PDFSVTNHIREALFLPGNMQAIKAFETFREKAVHQALVMDEFGGVLGLVTLYDVLESI
                     VGAIPLNEFDLNQEVVLRPDGSWSFDGLLPIDVLKDLLKVEQFPEEDTADFQTLSGFI
                     MNQLGHIPKVGQSFVWEQFSFEVLDMDGFRVDRVLVKDHSAEINETINEESET"
     gene            complement(3571..4320)
                     /locus_tag="PK791_02550"
     CDS             complement(3571..4320)
                     /locus_tag="PK791_02550"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOH92226.1"
                     /translation="MQLYFIRHAQSTNNARWESGEFTDNPRVADPPLTDKGILQSQLV
                     AQELSKHNPGIKTHFLDQQNRSGYGITHVYCSLMERAIQTGSIIARHLDVPLIALPDC
                     HEVGGIYLERIVGDKPVISMEYGLNASYIQATYPFLRLEHHIHEKGWWPGGKEDRELV
                     AERATRVIDFIRHRHGETDDRVALITHGGFFNYLFRALCRCEMNFAADQRYPYRFMYN
                     NCGISRFDMPDEMAQFIYHNKTDFLPDELIT"
     gene            4438..5514
                     /gene="prfA"
                     /locus_tag="PK791_02555"
     CDS             4438..5514
                     /gene="prfA"
                     /locus_tag="PK791_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOH92227.1"
                     /translation="MLEKLNAILDRYNDIEAQLAQVGEDYQLAVELSKERADLEDVAV
                     AARDYLETINKIDETKELIEIAEGELLDLARLELDELQEHRDEMEDKLRAMLVPKDPR
                     DNRNVIVEIRAGTGGDEAGLFVADLLRMYLKYADIHGYKTEIISSNETGIGGFKEVTF
                     TVKGKGAFSRLKFESGVHRVQRVPETESQGRIHTSTVTVAVLAEVDDFEIDIPDSDIE
                     IDVYKSAGAGGQNVQKNMTAVRIHHIPTGIVVACQDERSQLQNKTRAMSVLKARLYEL
                     EEQKRQSELDATRRSQIGTGERSEKIRTYNYPQSRVTDHRINQSSFNIQAVMDGYDLD
                     DFIEELQHVEEAERLASFETDATD"
     gene            5501..>5921
                     /locus_tag="PK791_02560"
     CDS             5501..>5921
                     /locus_tag="PK791_02560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HOH92228.1"
                     /translation="MLQISIRDCLLEAQAELSLLDNPQHSAIILMAHVLNRPKTWIIA
                     HGNETLGERQKTEFFALTKRLAQGEPLPYITGKQSFYGLDFKVSPSVLIPRPETERLV
                     DKALLWLRANPLASHALDVGTGSACIAVSLAKHHPALT"
BASE COUNT         1629 a         1543 c         1258 g         1491 t
ORIGIN      
        1 gaatccggtc acgatacccg gcacatattg cacctgctca ttacttactt gccagtcgta
       61 caatcgtgcc agtcggctgg taaccacatc ggtcaagcca tcgggcgcga aaccatagcc
      121 caaaatgcca tggtcaaccc gggcgtggat agcatccata acttcctgtg gcacttcaaa
      181 atcggtgtcc gccacccaca tcgccaaaat gtccgggtcc cagcgacgcc atttcaggct
      241 ttcagtctgt cttcggtcaa tatttttatc aaaattcatg cttactccgt ttctccaaaa
      301 attacttcat ttatgataac aaaatttagc catgttaggg aagaattggt atttttcaaa
      361 ttgttccacc cgactattac gcgcatttgg tatacttttt aacaagccat gaaaaagatt
      421 tttcctgcct gcatgaaact attgcgttta aagactggcc tggcggtgac gctctttgcc
      481 agcctgctcg gtgttgcagc cagccaggta acaccaaatt ggaagatgct ctgtataatc
      541 ctggcaaaca tttttagttc ctggatttta tttttgttcc gcgactggca gtacgcccca
      601 caagatatca gcatacaagc cgatgacatg caaaacccta tcgcggcggg tattattaaa
      661 ctcaacaccg cgaagttatt attgctggta cttgtcggca tcacgattac gctttatgcc
      721 ataccaggca ccaaaacgct tatcactggc ttggcgctct tagctatcat ttttatcctg
      781 aactggcgca aactgggctt gcaaaaaata ttccatctaa aaacaagccc ctacaactgg
      841 cttgtaaacg ctttcttctg cctgctgggc agtctcggtc ttgcagatga actaaatcag
      901 ttttcgatgc ttgccattgc tttcgttgta ttgattatgc tttacatcag cctgattgac
      961 caagccaggc aaggtaaaag tgcccgtacc ttacgacgcc tgaatatcat ctccgcgatt
     1021 tgcctttcac ttgccagtat cattggtatc atgctgttca ttatctttga attgctccct
     1081 tactgggtta cgggtttgtc attgatatta atggccatta tggtcgttcc ccaaacactt
     1141 gaacgtttcc gcgccaaaga aaagcctctt ttcgaaaatg accttttgcg cgttgccttc
     1201 ttccgaagtg gcgcattggg cctgatggtc catttcctcg ttccactaat atacagcctt
     1261 tttataaagt gagttgctat gaaacaaaac atccaaccac actattttga aaccgctact
     1321 gcctggcatg aacatgaact tctcgactct ggcgatggca aaatcctgga aagattcggc
     1381 aaaatggtac tcgtccgtcc ctacgcgcag gctttttggc caaaatccac gcccgaactt
     1441 tggcaggaaa accatgcccg gtttcaggct gacctccaca atttcggggg caaatggctt
     1501 tacaaagaca acgcgcaaaa atcctggccc ctgcgctggg aaatgctacg tttccagaca
     1561 caactgggtg cctcacaaca catcggcgtt tttccggaag aaattaagca ctggctatgg
     1621 ctccaggaca cgattaaaaa agcccaaaat cccgtcaaag tgctccattt gtgggcctac
     1681 acgggcttga tgaatctcgt cgccacccat gctggcgcgg acgtgaccca tgtggacagc
     1741 tcaaaacatg gcatcggctg ggcgatggca aaccagaagc tttccagcag gaaagacgcc
     1801 cccatccgat acattcagga agagcccctt aagttcatca agcgggaagc ccggcgcgaa
     1861 aaaaactaca acgcgctaat tctgcaattc ccacgaatgg aaaatgaaga agaatcgcgt
     1921 tacactgatt ttctcaaaca gctaccagac ctgattctcg ccatcaaaga cattctcgac
     1981 ccaaatccac agtttattct cattaccgca cccgcttgca ttcatccagt cacaactcag
     2041 gcaattgtgg atgatctaaa accaggtcgg ggaacaataa gcagtggcgt catttgcctt
     2101 aaagaaaaaa gcgcagggca tcttttgccc cacgcttcgt ttacacgatg gtccagtaat
     2161 taggtttcgc tttcttcgtt tatcgtttca ttgatctcgg cagagtgatc tttgaccaaa
     2221 acacggtcga ctctaaatcc gtccatatct aaaacttcaa acgagaactg ttcccaaaca
     2281 aaactctgcc caacttttgg gatatgccca agctggttca taataaagcc actcaatgtt
     2341 tggaagtcgg cagtatcttc ttcagggaac tgttccacct taagtaagtc ctttaggaca
     2401 tcaatcggca aaaggccatc aaacgaccac gaaccatcag gacgtaaaac cacttcctgg
     2461 ttaaggtcaa actcatttag gggaatggcg cccacaatcg attcaagtac atcatataag
     2521 gtaaccaggc ccaaaacgcc accgaattcg tccataacca aagcctgatg caccgctttc
     2581 tctcggaacg tttcaaacgc ctttatcgcc tgcatattgc ctggcaaaaa cagcgcctcg
     2641 cggatatggt tggtaaccga aaaatcaggc gcgtaaatat ccacacccac taaatcctta
     2701 atgtgcaaca taccgatagt gcggtccaaa tcgccctcag ctactggcat acgcgaataa
     2761 ggactcgcct tgatatcatg aataatttcc tcttgctcat cctcgatatt aatccagtcc
     2821 agttcggtat ggggcgtcat cagggcgtca atccgccgtt ctgtaagacg aaacacgcca
     2881 tccaccatct cttgttcagc ctggtcaaag acgccagtct ggcgaccttc ttcaataaag
     2941 cctttcacat cttcttcagt aatggatggt tctgggctga gttcaatgcg caagacacgc
     3001 agaccgatgt tagtggaacc acttaacaat cgtgttaaag gttttaagat tcgcgttagg
     3061 gtcctgacca tcggggaaag tgccgtaacc acattttcgg gttcactgac ggcaattctt
     3121 ttgggaatta gctctccaag cacaatcgaa aaataggtaa tcagaatgac cacaattaaa
     3181 agcgcgaacg tttccgcgat aggtgccagc catgtgactt gcttcataat ctccgccaaa
     3241 ggttgcgaga taacagcacc accgatacca ccagccaggg tatcaatcat ggtgatcata
     3301 atttgaattg ccgaaagata ttcctgatta ggatctttta gtaattccaa agcgtaggct
     3361 gcgccttttt taccttcagc aacacgttgc tccagtctga cttttcgtgc ggacaccata
     3421 gcgatttcat acatcgcaaa aacggcattc aacccgaaaa ggatgaagat aaatatgata
     3481 ttgaaaatta ttggcaagat attcctcttt tagtctatgc tttctaggtt aacggtgcat
     3541 agctgttgct attctaccaa gctttacaga ttacgtaatc aattcgtcgg gtaaaaagtc
     3601 ggttttgtta tgatagatga actgcgccat ctcatcgggc atatcaaaac gactaatacc
     3661 gcaattattg tacataaacc gatacgggta gcgttggtca gcggcaaagt tcatctcaca
     3721 gcgacaaagt gcccgaaaca gatagttaaa aaagccacca tgcgtaataa gcgccacacg
     3781 gtcgtctgtt tcgccatggc gatgacgaat aaagtcaatc acccgcgtgg ctctttcggc
     3841 aacgagttcg cggtcttcct tgccacccgg ccaccaaccc ttttcatgga tatgatgctc
     3901 aaggcgtaaa aagggatatg tcgcctgaat ataactcgcg ttcagtccat actccatgct
     3961 tatcacgggc ttatcgccca ctattctttc caggtaaatg ccacctactt catgacagtc
     4021 aggcagcgcg atgagtggta cgtccaaatg ccgggcaata atactgccag tctgaatggc
     4081 gcgttccatc aaactgcaat agacatgagt gattccatac ccacttcggt tttgctggtc
     4141 taaaaagtga gttttaatac cagggttgtg tttgctcagt tcctgcgcca ccagttggga
     4201 ttgcaaaata cccttgtccg ttaatggcgg gtcagccaca cgcgggttat cggtaaactc
     4261 accactttcc cagcgtgcgt tattcgttga ttgagcgtgt ctaataaaat atagttgcat
     4321 cctaaacttt ctccgacggc tgattataca cggaatggca aaaaatacga aaggtttata
     4381 gtaatacatc tctccaaatg gtataatcgg gctgaaaatt gtaaaggcac taagaatatg
     4441 ttagaaaaat taaacgcaat actcgaccgc tataacgaca ttgaagcaca attagcacaa
     4501 gttggcgaag attatcaact ggctgttgaa ctttccaaag agcgtgctga ccttgaagat
     4561 gtcgcggtag ctgcccggga ttatcttgaa actataaaca aaattgatga gaccaaagaa
     4621 ctcattgaga tcgccgaagg cgaattactc gaccttgccc gcctggagct tgatgaactc
     4681 caagagcacc gcgacgaaat ggaagataaa ctccgcgcca tgctcgtgcc caaagacccg
     4741 cgcgacaacc gcaacgtcat cgttgaaatt cgcgccggca ccggtggaga cgaagccgga
     4801 ctcttcgtgg cagatttgtt acgtatgtat ctaaaatacg ctgacatcca cggttataaa
     4861 actgagataa tttcttccaa cgaaactggc atcggcggtt ttaaagaagt gaccttcacc
     4921 gtaaaaggca aaggcgcttt ttcgcgttta aagttcgaat cgggcgtcca ccgtgttcaa
     4981 cgcgtgcccg aaaccgaatc ccaggggcgt attcatactt ccactgttac cgttgccgtg
     5041 ttggccgaag tcgatgactt tgaaatcgac atccctgatt cagacattga aatcgatgtc
     5101 tacaaatcag ccggcgcggg tggccaaaac gttcagaaga acatgactgc cgtgcgcatc
     5161 caccacatac ccaccggtat cgttgttgcc tgtcaggacg aacgctcaca actgcaaaac
     5221 aaaacccgcg cgatgagcgt gctcaaagcc cggctttacg aacttgaaga gcaaaagcgg
     5281 caatccgaac ttgacgctac ccgtcgctcg caaattggca ccggcgaacg cagcgaaaag
     5341 attcgcacct ataattatcc tcaatcgcgc gtcaccgacc accgcatcaa tcaaagttcc
     5401 tttaacattc aggccgtcat ggatggttac gacctggacg attttatcga agaactgcaa
     5461 catgttgaag aagccgagcg attggcaagt tttgaaaccg atgctacaga ttagcattcg
     5521 tgactgcctg ctggaagcgc aagctgaact cagtcttttg gataaccccc agcatagtgc
     5581 cattatcctg atggcacatg tcttaaatcg accaaaaacc tggattatag cccatgggaa
     5641 cgaaacgctt ggcgagcgcc aaaaaacaga gtttttcgcc cttacaaagc gtttagcaca
     5701 aggcgagccc cttccctaca tcaccggcaa gcaaagtttt tacgggctcg attttaaagt
     5761 cagcccgtct gtactcatcc cacgccctga aacagaacgc ctggttgaca aagctttgct
     5821 ttggctacgc gcaaacccct tggcgagtca cgcgctcgat gtcggcaccg gttccgcttg
     5881 tatcgcggta agcctggcca aacatcaccc tgccctaacc a
//
LOCUS       DAOSFC010000061         5637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_12688_length_5637_cov_4.383196, whole genome shotgun sequence.
ACCESSION   DAOSFC010000061 DAOSFC010000000
VERSION     DAOSFC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12688_length_5637_cov_4.383196"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1241)
                     /locus_tag="PK791_02565"
     CDS             complement(111..1241)
                     /locus_tag="PK791_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92229.1"
                     /translation="MLEIIPVSDKKTKKEFVEFQYTLYKDVPQFVPPFKADVYMQLNK
                     EKHPWYEHSESDQWLAKRDGQVVGRIAALENKRFNDTHNSKQASFYLFECINDQEVAN
                     ALFDTAIAWAKSRGLDKMVGPKGYGPLDGYGIQIEGNEHRQMMNMMNYNFLYYKDLVE
                     NYGFSKEVDFVSSFVRPQEYVLPEKVKKAVEIVKKRGRLKVKSFKNKREITQWKTRIK
                     DAYNNAFVQNWEYYPLTDNELEYVLNNALLVVIPTMVQVILNENDDLVGFVLPFPDIS
                     RAMQKGKGKMGPIEIIRLLRELKKTDWVCYNGIAILPEYQGMGGNALLFSTLLDATQK
                     HPYLVNGELSQVAETAVQMRKDLLNLGVKPYKNHRVYTIDIV"
     gene            1445..2002
                     /gene="efp"
                     /locus_tag="PK791_02570"
     CDS             1445..2002
                     /gene="efp"
                     /locus_tag="PK791_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOH92230.1"
                     /translation="MIDVNDLRKGVTFIQDNNLYRVLEYSHNKPGRGSATIRIKARDL
                     RKGTTLEMTFISGDRVQSVRLDYHRVEYLYRDDNFFHFMDMETFEQPAIPKDLVEDIE
                     GYLKENMEAKLTFYGEEPLDIELPTSVDLLVTTAEMAARGNTATGLTKQVEVETGLKV
                     QVPAFVTEGDTIRIDTRTGEYVTRV"
     gene            2445..4307
                     /gene="ftsH"
                     /locus_tag="PK791_02575"
     CDS             2445..4307
                     /gene="ftsH"
                     /locus_tag="PK791_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOH92231.1"
                     /translation="MKPRRQSFLITGLLLLAIVMMAFYGLRQQTIGSEVVPINRVATQ
                     LNDGKISKLEADGDTLYITYDDPKKSFKSTLDPKMGLFDQLTQLGVTEEALQNPALEI
                     RVRLPGFWSEFFLVAVQFIPFILMGVLFWIFIRQAQGSNNAAMQFGKSRARMLSGEQS
                     NVTFADVAGVDEAKEELQEVVEFLKEPQKFMALGARIPKGVLLVGPPGTGKTLMAKAV
                     SGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDEAKKRSPCIVFVDEIDAVGRQRGAG
                     LGGSHDEREQTLNQLLVEMDGFGTDTNVIVMAATNRPDILDPALLRPGRFDRRVMLDK
                     PDMKGREDILKVHSRGKPISNDVDLGVLARGTPGFAGADLENLVNEAALLAARRDKKV
                     IGNDEFEEAIERVIAGPERKSRLMNQEEKRLTAYHEAGHAIVITALPEGDPIQKVSII
                     SRGMMGGFTLALPVEELNYKSKKMILADITGLLGGRAAEELMANDITSGASNDIERVT
                     KLARAMVTRLGMSEEMGPMVYGQKEELVFLGREISEQRDYSEAVAERIDAEVARIVNE
                     CYQHALDILTEHKAKLIEVAEKLLEQETLSKEEFDAIFPSPNEKTYGVPVIVTD"
     gene            complement(4297..5244)
                     /gene="miaA"
                     /locus_tag="PK791_02580"
     CDS             complement(4297..5244)
                     /gene="miaA"
                     /locus_tag="PK791_02580"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOH92232.1"
                     /translation="MTKDINKTPIIFLLGPTASGKTSLAIRLAQELDTEIISADSRYL
                     YRELNIGTAKPTEAELKMVKHHLIDVTSVSEIWSLGEYLKAIKEPINNLHSKGKIPVL
                     VGGTGQYYRAIAQGWQVPKLPPNPALRTAIENWGNDIGFDALHQKLSYIDPNAAKNID
                     HRNHRRTVRAWEVILATGELFSEQRKKWDTPYKTLSIGIEWDRPILYERIDKRIDKMF
                     ENGLIDEVKDLMNKGLTDAVKKIGVIGYAETIAYLENEISFDDCIMLIKRHTRQFVRR
                     QANWFKPSDPNIHWFKATDPAYYEKILGLIDAFMVSANQ"
     gene            complement(5237..>5637)
                     /locus_tag="PK791_02585"
     CDS             complement(5237..>5637)
                     /locus_tag="PK791_02585"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOH92233.1"
                     /translation="MLDILIKMAKTYRQRIGAMGEDIALNWLNEKGYELVQRNFRFKN
                     DEIDLIMLKDDVLFFVEVKTRRNDNYGLAEYAMTPKKLTALFRCIDHYLYTQDKLEIE
                     WQLDLIVVEKFQKDKAPKIFHFENLGLYDD"
BASE COUNT         1583 a         1194 c         1129 g         1731 t
ORIGIN      
        1 aaggcattag cttcccaagc taacattcgt gggttcgaat cccatcaccc gctttaaaaa
       61 acaactctgg caattatctc ctgccagagt tgttctttta atgattttat ttatacaatg
      121 tcaatcgtat aaactcgatg gttcttgtaa ggttttacac caagattcaa taaatctttg
      181 cgcatttgca ctgctgtttc agcgacctgc gaaagttcac cattcactaa atacggatgt
      241 ttttgtgtcg catccaacaa cgtgctaaac aacaaggcat tgccacccat gccctggtat
      301 tccggcagaa tagcaattcc attgtaacag acccaatcag ttttctttaa ttcacgtaac
      361 aatcgaataa tttcgatagg ccccatcttg cctttgccct tttgcatggc acgcgaaata
      421 tctgggaatg gcaacacaaa tcctaccagg tcgtcatttt cattcaaaat cacctgaacc
      481 attgttggaa taacaaccag caaggcatta ttcagaacat attctaattc gttgtccgtc
      541 agaggataat attcccagtt ttgaacgaaa gcgttgttat aggcatcttt tatccgtgtt
      601 ttccactgcg taatctcgcg tttgttcttg aagcttttta cttttaatct tccgcgcttt
      661 ttaacaattt caacagcttt tttaaccttc tcaggtaaga catactcttg cggtcttaca
      721 aagctgctta caaagtcaac ttccttacta aaaccatagt tttcaactaa gtctttgtaa
      781 tataagaagt tgtagttcat catgttcatc atttggcgat gttcatttcc ttcaatttgg
      841 attccatagc catctaatgg accgtagcct tttgggccaa ccattttatc taagccacgc
      901 gatttcgccc atgcaatagc agtgtcaaaa agggcgttgg caacttcctg gtcgttaata
      961 cattcgaaaa ggtaaaaaga agcttgtttt gaattgtggg tgtcattaaa gcgtttgttt
     1021 tcaagagcgg caatacgtcc aacaacttgt ccatctcgct tggctaacca ctggtctgat
     1081 tcagaatgtt cataccaggg atgcttttct ttattgagtt gcatataaac atcggctttg
     1141 aatggtggaa caaattgtgg aacatctttg tataaagtgt attgaaattc gacaaattct
     1201 ttctttgtct ttttatcaga tactggaata atttctagca ttatgtatac tcccttcaaa
     1261 atgttataac ttttttatac ctgttacggt atattaataa atataaccca tcttgttgat
     1321 aaaagatacc aaataaatac aataaatcga tttcacattc cgctgtataa gatataaatt
     1381 acttgcgatg aagcgatcgt tgtactataa tggcgtcgtt ttattcagga agaaagagga
     1441 ctatatgatt gatgtaaacg atttaagaaa aggtgttact tttatccagg acaataacct
     1501 ttaccgtgtt ttggaatatt cacacaacaa accaggccgt ggttcggcta ctattcgcat
     1561 caaggcacgt gatctgcgca aaggaaccac tctcgaaatg accttcatct ctggtgaccg
     1621 tgttcaatcc gtgcgcttgg attaccaccg tgttgagtat ctctaccgcg atgataactt
     1681 cttccatttt atggatatgg aaacgtttga acagcctgct attcccaaag atttggttga
     1741 ggatattgaa ggttatttga aagagaacat ggaagccaag ctaaccttct acggtgaaga
     1801 acctttagat attgaattac ccacaagtgt tgatttactt gttacaaccg cagaaatggc
     1861 tgcgcgtggc aataccgcta ctggattgac caaacaggtt gaagtggaaa ctggacttaa
     1921 ggtacaagtt cccgctttcg ttaccgaagg agataccatt cgcatcgaca cacgtactgg
     1981 tgaatacgta acacgggtgt aatttgcgct tgaaactctt tttaacaaaa caaatagtat
     2041 gaaaacacct ttcggacacg aatgccgctt tttctatggt gattactatc ggggtcggaa
     2101 ttttgaagaa tgtcggggtt taatcaaccc gaacgacaaa gcagaatgga catctttact
     2161 gtgcgaaacc tgccccgttc ctaacataca gcggaataac aattgcccta atcttgtttt
     2221 gactactttt ataagtaaat cttggtttaa aaaacgtatc aagatcaaag caagttgcag
     2281 caaaacttta gaatcggtaa aggaccccaa cgttggctgc ggtttatgcc acacaccgta
     2341 agaaaaccgt aagaatatct ttgcattatt gcagacattt tcgtgtaaag attcctgctt
     2401 ggtataattc aacatgacag atatgtattg aacatggagg cttagtgaaa cctagacgac
     2461 aatcattctt aataacagga cttttgcttt tggcaatcgt tatgatggcc ttttatggct
     2521 tacgacaaca aactatcggt tctgaagtcg tgcctataaa tcgggttgcg acacaattaa
     2581 atgatgggaa aatatccaaa cttgaggcag atggggacac gctttacatc acttatgacg
     2641 accccaaaaa gagttttaaa tcaacacttg accccaagat gggccttttt gaccaactca
     2701 ctcaacttgg tgtgactgaa gaagccttac aaaaccctgc ccttgaaatc cgagtccgct
     2761 tacctggatt ttggtcagaa ttctttttag ttgcagttca attcataccg tttatcctaa
     2821 tgggcgttct cttttggata tttattcgcc aggcacaagg ttcaaataac gctgccatgc
     2881 agtttggcaa atcgcgagcc cgaatgctta gtggagaaca atctaacgtc acctttgctg
     2941 atgttgctgg cgtagacgaa gccaaagaag aacttcagga agttgtcgaa ttcctaaaag
     3001 agcctcaaaa atttatggca cttggcgctc gtatccctaa aggagtttta cttgtaggcc
     3061 ctcctggcac aggtaaaacg cttatggcga aagctgtatc tggtgaagct ggcgtgcctt
     3121 tcttttctat ttctggttct gaatttgttg aaatgttcgt cggtgtcggt gccagtcgtg
     3181 tgcgtgacct gtttgatgaa gccaaaaagc ggtccccttg tattgttttc gttgacgaaa
     3241 ttgatgccgt gggacgccag cgtggtgctg gtttgggtgg cagtcatgat gaacgcgaac
     3301 aaacattgaa ccaactcctg gttgaaatgg acggttttgg aacagataca aacgtgattg
     3361 ttatggctgc aacaaaccga cccgacattc ttgaccccgc cttattgcgt cctggacgtt
     3421 ttgaccgtcg tgtcatgctg gataaacccg acatgaaagg acgcgaagat attctaaaag
     3481 tccactctcg cggcaaaccc atatctaacg acgttgatct gggcgtcctt gcacgcggca
     3541 cacctggttt tgctggcgct gatttagaaa acctggtcaa cgaagccgcc cttttagctg
     3601 cccgacgcga taagaaggta atcggcaatg atgagttcga agaagccatt gaacgtgtta
     3661 tcgccggccc tgaacgcaag agccgcttga tgaaccagga agaaaaacgc ctgacagcct
     3721 atcatgaagc tggacacgct atcgtgatta ccgctctacc cgaaggagat cccattcaaa
     3781 aagtttctat tatctcccgt ggcatgatgg gcggttttac acttgctctt cccgttgaag
     3841 aattgaacta taaatccaag aaaatgattt tggcggatat caccggttta cttggcggac
     3901 gtgcggcgga agagttaatg gccaacgata tcacatctgg tgcatcaaac gacatcgaac
     3961 gtgttaccaa actcgctcgt gcgatggtta cacgcctggg tatgagtgaa gagatgggtc
     4021 caatggttta cggacagaaa gaagaattag tcttccttgg tcgcgaaata tctgaacaac
     4081 gcgactactc agaggccgtt gccgagagaa ttgacgcaga agtcgcccgc attgtcaatg
     4141 aatgttatca gcacgctttg gatattctga ccgaacacaa ggccaaactt attgaagtcg
     4201 ctgaaaagtt acttgaacaa gaaacgcttt ctaaagagga atttgatgcg attttcccat
     4261 cgccaaatga aaaaacatac ggtgtaccgg taatcgttac tgattagcag ataccataaa
     4321 agcatctatc agacccagta tcttttcata ataggctggg tctgttgctt taaaccaatg
     4381 gatgttggga tcactgggtt tgaaccagtt ggcctgtcta cggacaaatt gacgtgtatg
     4441 gcgttttatt agcattatgc aatcatcaaa gctgatttca ttttccaaat aagctattgt
     4501 ttcagcatag cctatcactc caattttctt gactgcatct gtcaagcctt tattcatcaa
     4561 gtctttgact tcatcgatta atccattctc aaacatttta tcaatacgct tgtcaatccg
     4621 ttcgtacaat atcggtctat cccattcaat gccaatagat aatgttttgt atggcgtatc
     4681 ccattttttc ctttgttcag aaaagagttc cccagtcgct aaaattactt cccatgccct
     4741 aacagtgcga cgatgattgc gatggtcaat atttttagct gcgttggggt cgatgtaaga
     4801 taatttctgg tgtaatgcat cgaagccgat atcattgccc caattctcaa tcgcagtccg
     4861 taaagctgga tttggcggta attttggcac ctgccagcct tgtgcaatcg cgcgatagta
     4921 ctgacctgtt ccgccaacca aaacaggtat ttttcctttt gagtgtaaat tgtttatagg
     4981 ttctttgata gcttttagat actcaccaag actccatatt tcgcttacgc ttgtaacatc
     5041 aattaaatga tgtttgacca ttttaagttc cgcttctgtc ggttttgcag tgccgatatt
     5101 taattcgcgg taaaggtatc gtgaatctgc tgaaattatt tcagtgtcta gttcttgtgc
     5161 caaacgtatc gccagagatg tttttccaga agctgttggc ccaagcagaa aaataatcgg
     5221 ggttttattt atgtctttag tcatcgtaca atcccaaatt ttcaaagtga aatattttag
     5281 gtgctttatc tttttggaat ttttcgacta caatcaaatc taattgccat tctatttcta
     5341 atttatcttg cgtgtacaaa taatgatcaa tacaacgaaa taatgctgtc aacttttttg
     5401 gtgtcattgc atattcagct aaaccgtaat tatcgttacg tcgggtttta acttcgacaa
     5461 aaaacagtac atcgtcttta agcataatca ggtcgatttc atcattttta aaccggaagt
     5521 tccgctgaac aagctcatag cctttttcat tcagccagtt taaagcgata tcttctccca
     5581 ttgcacctat tctttgtcgg taagtttttg ccattttaat aagtatatca agcatgt
//
LOCUS       DAOSFC010000062         5629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_12715_length_5629_cov_5.837280, whole genome shotgun sequence.
ACCESSION   DAOSFC010000062 DAOSFC010000000
VERSION     DAOSFC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12715_length_5629_cov_5.837280"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PK791_02590"
     CDS             complement(<1..605)
                     /locus_tag="PK791_02590"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOH92234.1"
                     /translation="MNEKLKETLLSIMPITVMVLLIHFSTYAALDSVLLTRFIIGAVM
                     MMIGLPIFLQGVDMSIERMGEQMSGALVKSNKMIIVLIGGFILGFLVNAAEPDVHIFS
                     RQFAALTAPELSHWIFVLIMSAGFGLAVAAGLLRIIRNIKMRYFMAIFFGLLFVLTLF
                     TGEEFIGVAFDTYGATTGAIATPFLLALAIGVSSKTRRSTD"
     gene            complement(756..1175)
                     /locus_tag="PK791_02595"
     CDS             complement(756..1175)
                     /locus_tag="PK791_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOH92235.1"
                     /translation="MQAKMNWKENMHFQGSTESDFIVDLDVPAADGKNSKSLTPMQLI
                     AMGVAGCTSMDVISILEKRRLDVRDFSVQVEVERRDEIPRVFTHLHFIYTVTGKNIKR
                     KDVEFAVKLSEEKYCQGIAMMRASGAGISNEIVINEI"
     gene            complement(1234..2268)
                     /gene="tsaD"
                     /locus_tag="PK791_02600"
     CDS             complement(1234..2268)
                     /gene="tsaD"
                     /locus_tag="PK791_02600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOH92236.1"
                     /translation="MKQAHLRVLGIESSCDETAAAIVEGGRVIHSNVVASQIDLHAQY
                     GGVYPELASREHVRAIYPTVEAALKNAYMNLSEIDAIAVTQGPGLAGSLVIGVNAAKA
                     MALASGKPLIPVNHLEAHIYAAWLHKAGSKPGPEPKFPLIALLVSGGHTELVLMSDHL
                     QYQSLGSTLDDAAGEAFDKVARLLNLSYPGGPAIQAAALDGNPEAYKFPIARLDSPWD
                     FSFSGLKTAVMRQVDAYKDFATDVPVADIAASFQATVVETLFSKTMAAAETHKAREII
                     VAGGVSANRALRDAFRGQNKFKVHIPHVSLCTDNAAMIAAAGFRHLVNGESYGLDFDV
                     LPNWPLSTSA"
     gene            complement(2273..3511)
                     /locus_tag="PK791_02605"
     CDS             complement(2273..3511)
                     /locus_tag="PK791_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOH92237.1"
                     /translation="MKKDIDQLMQKHNVDVIWVRGAATNNPTMYYLTGGGHITNADLF
                     YRAGKTPILMHGTMERDEAAKTGYELLSYANYPIMDLLKQCDGDLAQATVLQFKQILS
                     DLGITQGRVMLYGKQEISKVLPLIQGLSKALPEIEFIGDMDDTILLEARATKSPDEIE
                     RIRQMGVRTTTVVGKTARFLQSHPVGENETLIKADGSPLTIGDVKSQIKLWLAKAGAE
                     DTEGMIFAIGRDAGVPHSSGNPDDPITLGRTIVFDIFPAEAGGGYFYDFTRTWCLGYA
                     SAEVEKVYNDVVKVYDTVVGELKLGVNAAEYQARTCELYEAMGYETIRQNPRLEQGYI
                     HSLGHGLGLNVHEKPWFARKEDPSNVLQVGSVFTIEPGLYYPEKGFGIRVEDTWYVKE
                     DGSFEKLVDFPRDLVLPMKG"
     gene            complement(3519..4868)
                     /gene="selA"
                     /locus_tag="PK791_02610"
     CDS             complement(3519..4868)
                     /gene="selA"
                     /locus_tag="PK791_02610"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOH92238.1"
                     /translation="MADFRQIPSVDQLIQSDNFQATLQSYGHDWTVSAIRETLNEIRS
                     ALKAGKALPSHAEIAQSVNQRLAAQSMPELQNVINATGVLLHTNLGRAPLSQAASQAA
                     SDLSQTYSSLEFNLSTGKRGKRESHCAEFISRLSGAEDALVVNNNAGAVLLALSALAK
                     GKKVAISRAQLVEIGGGFRIPDVMRQSGAKLVEIGTTNRTHLKDYEEAIGQGARLVMT
                     AHQSNFQILGFTTEPSLTEICALAKSAGLPVYLDLGSGSFINMEQYGLAHEPTVPEVI
                     AQGADLVSFSGDKLLGGPQAGILAGKKVLIDKIRKHPLYRALRPDKLCLAALSATLLH
                     YLKGDYEQEIPLYRMLSQSEVQIKQRAQAWQAQLPNSQIIPGESTIGGGSLPGETLPT
                     WLLQFKPKSANKLLNALRAGKPPIIARIQEQSVVLDPRTVQAEEELIIIQRLKEFLE"
     gene            complement(4871..5281)
                     /locus_tag="PK791_02615"
     CDS             complement(4871..5281)
                     /locus_tag="PK791_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92239.1"
                     /translation="MTRAYKRTSFIPWLPAFFALLIIGGGGMAVILTQTLPTLGPRWL
                     FFFFLMLFASGFFLPLAWFINLRFPSNPPAESIVIIRQAILGGIFVSLLAWLQMGRVL
                     TLLLGGIILIVLIVIEFFLRLWERSRWKPNQEAD"
     gene            complement(5411..5499)
                     /locus_tag="PK791_02620"
     tRNA            complement(5411..5499)
                     /locus_tag="PK791_02620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5463..5465),aa:Ser,seq:gct)
     gene            complement(5525..5609)
                     /locus_tag="PK791_02625"
     tRNA            complement(5525..5609)
                     /locus_tag="PK791_02625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5573..5575),aa:Ser,seq:tga)
BASE COUNT         1267 a         1384 c         1555 g         1423 t
ORIGIN      
        1 tcatcggtac tgcgtctggt tttggaagac acaccaatgg cgagtgccaa aagaaaggga
       61 gtggcaatag cgccggttgt ggcgccgtag gtatcgaaag ccacgccgat gaactcttcg
      121 ccggtaaaaa gggtgagcac aaacagcaaa ccaaagaaaa tagccataaa atagcgcatt
      181 ttgatgttgc gaatgatgcg caagagacct gcggcgactg ccaggccaaa accggcagac
      241 atgataagca cgaaaatcca atggctgagt tctggcgcgg tgagcgcggc aaactggcgc
      301 gaaaagatat gcacgtctgg ttcagcggcg ttgaccaaaa agccaagaat aaagccacca
      361 atgagcacga taatcatttt gttcgacttg accaacgcgc cactcatctg ctcgcccatg
      421 cgttcgatac tcatatcaac gccttgcagg aagatgggca ggccgatcat catcatcact
      481 gcgccaatga taaagcgcgt aagcaagacg gaatcaagtg ctgcgtacgt gctaaagtga
      541 atcaaaagca ccataacggt gataggcatg atggaaagaa gagtttcttt tagtttttca
      601 ttcatttagt cgcgatcctt tacacgaatt ttgattaatg acgaatcgtg ttgagtatac
      661 caaagaatga ccagttattt gggttttact ggtcattctt gagaaacaga accgaatatt
      721 tgggggttgt gcgttaggct tttggccttt gaatattaaa tttcgttaat cacaatctcg
      781 ttgctaatgc ctgctccact ggcgcgcatc atggcgatgc cctggcagta tttttcttcg
      841 gaaagcttaa cagcgaattc gacgtccttg cgtttgatgt ttttgccagt cacggtgtag
      901 ataaaatgta agtgggtgaa gacacgcggg atttcatcgc ggcgctcaac ttcaacctga
      961 acactaaaat cccgaacgtc caaacgccgt ttttcgagaa tactgattac gtccatgctt
     1021 gtgcaaccag caacgcccat ggcgatgagt tgcatagggg taaggctttt gctgttttta
     1081 ccatctgctg cgggaacatc aaggtcaacg ataaagtcac tttcagtgct accttgaaag
     1141 tgcatatttt ctttccagtt catttttgct tgcatatgtc ctccaatgtt tgcttatcta
     1201 gtgcatctat tttcggacaa ttcgacttgg ttttcaagcg cttgtagaga gtggccagtt
     1261 gggcaggacg tcaaaatcaa gcccgtagct ttctccgtta accaaatgcc taaatccagc
     1321 cgcggcaatc atggcggcgt tatcggtaca gagactgacg tgcgggatat gcactttaaa
     1381 tttgttttgc ccgcggaaag cgtcgcgcag ggctctgttc gcgctgaccc cgccggcaac
     1441 gatgatttcc cgggctttgt gcgtttcggc cgcggccatg gttttgctaa agagcgtttc
     1501 aacgacggtc gcctggaaac tggcggcgat gtctgccacg ggcacatcgg tggcaaagtc
     1561 cttataggcg tccacttggc gcatcaccgc ggttttgagc ccgctgaatg aaaaatccca
     1621 ggggctgtca agcctggcga tggggaactt atacgcttcg gggttgccat caagcgctgc
     1681 agcctggatg gcgggcccgc cgggataaga cagattgagc agtcgcgcta ctttgtcaaa
     1741 tgcttcgcct gccgcgtcgt caagcgtgga gccgaggctt tggtattgca ggtggtcgct
     1801 catcagcaca agttcggtgt gtccgccgga aaccaacaag gcgattaagg ggaatttggg
     1861 ttctggcccg ggtttgctgc cggctttgtg gagccaggca gcgtaaatat gggcttcgag
     1921 atggttaacg gggatgagcg gtttaccact ggcgagcgcc atcgctttgg cggcgttaac
     1981 gccaatcacc agggaaccgg ccagtcctgg cccctgggtg accgcgatgg cgtctatttc
     2041 cgaaagattc atgtaggcgt tttttagcgc ggcttccact gtggggtaaa tggcgcgcac
     2101 gtgctcgcgc gaagccagtt cggggtaaac gccaccatac tgggcgtgca ggtcaatttg
     2161 acttgccaca acattactat gaataacgcg cccaccttca acgatggcgg cggcggtttc
     2221 gtcgcaggaa ctttcgatgc ctaaaacgcg taaatgggct tgtttcataa gattatcctt
     2281 tcataggcag taccaggtcg cgtgggaagt ccacaagctt ttcaaaactg ccgtcttctt
     2341 tgacatacca ggtgtcttca acacggatgc caaatccttt ttcagggtag tagagaccgg
     2401 gctcgatggt gaatacggag ccaacctgca gcacgttgct ggggtcttct ttgcgcgcga
     2461 accagggctt ttcgtgaacg ttcagcccca ggccatgtcc caggctgtga atgtagcctt
     2521 gttccaggcg tgggttttgg cggatggttt cgtagcccat agcctcgtag agttcacagg
     2581 tgcgggcctg gtattccgcg gcattcacgc ccagctttaa ttctccaaca acggtgtcgt
     2641 aaaccttgac aacgtcgttg taaacttttt caacttcggc tgaggcgtat cccaggcacc
     2701 aggtgcgggt gaagtcgtaa aaataaccac caccggcttc ggcggggaaa atatcgaaaa
     2761 caatcgtgcg acccagcgtg atgggatcat ctgggttgcc actgctgtgg ggcacgccag
     2821 cgtcgcgtcc gatggcgaaa atcatgcctt cggtgtcttc cgcgccagct ttggcaagcc
     2881 atagtttgat ttggctctta acgtcgccaa tggtcagtgg gctgccgtcg gctttgatga
     2941 gcgtctcatt ttcgcccacg gggtgacttt gtaagaatcg agctgttttg cccacaacgg
     3001 tcgtggtgcg cacacccatt tggcgaatgc gttcaatttc gtcagggctt ttggtagcac
     3061 gcgcttccag caagatggtg tcgtccatat cgcctataaa ttcaatctcg ggcaaggctt
     3121 tgcttagtcc ttgaatcaag ggcagcactt tgctgatttc ctgcttgcca tagagcatca
     3181 ctctgccttg ggtgatgccc aaatcgctta ggatttgttt gaactgtaaa acggtcgcct
     3241 gagcaaggtc accatcgcat tgcttgagca aatccatgat cggatagttc gcgtagctaa
     3301 gtaattcata gccggtttta gcggcttcgt cgcgttccat agtgccgtgc atcagaatcg
     3361 gtgttttgcc agcgcgatag aaaaggtcag cgttggtgat gtgtccgcca ccggtcaggt
     3421 aatacatggt ggggttgttc gtcgcggcgc ctctaaccca gatgacgtct acgttgtgtt
     3481 tttgcatgag ttggtcgata tcttttttca tagttcctct attctaagaa ttcctttaaa
     3541 cgttggatga tgataagttc ttcttcagct tgcaccgtgc gggggtcgag caccacactt
     3601 tgctcttgaa tacgcgcgat gatggggggc tttcccgcgc ggagcgcatt gagcagtttg
     3661 tttgctgatt tgggcttaaa ttgaagcagc caggtgggca gtgtttcgcc gggcagacta
     3721 cccccgccga tggtcgattc acctggaatg atttggctgt ttggaagctg ggcttgccag
     3781 gcttgagccc tttgcttgat ttggacttca ctttgcgaaa gcatccggta gagcgggatt
     3841 tcctgctcat aatcgccttt tagatagtgc aaaagcgtgg cagaaagagc agccaggcac
     3901 agtttgtccg gacgcagggc gcgatacagg ggatgcttgc ggattttatc aatcagcact
     3961 tttttgccag ccaaaatgcc ggcttgtggg ccacccagta atttatcacc ggaaaaactg
     4021 accaggtcgg caccttgcgc gatgacttcc ggcacggtgg gctcgtgtgc cagtccgtac
     4081 tgttccatgt tgataaaaga gccggaaccc aggtcaagat agactggcag tccggcagat
     4141 ttggcgagtg cgcagatttc agtcaggctt ggctcggtgg taaaacccag aatttgaaaa
     4201 ttactttgat gggcggtcat caccagtcgc gcgccttgcc cgatggcttc ttcgtaatct
     4261 ttcaggtgtg ttcggttggt ggtgccgatt tcaacgagtt tcgcgccgga ttgtcgcata
     4321 acgtcgggaa tgcgaaaacc gccgccaatt tccaccagtt gcgcgcgcga gatggccacc
     4381 tttttgccct tggccagggc gctgagtgcc agcagtaccg cgccggcgtt gttgttgacc
     4441 acgagcgcgt cttccgcgcc actcagacgg ctgataaatt cagcgcagtg gctttcgcgc
     4501 ttgccccgtt tgccggttga aaggttaaac tccagcgagc tgtaagtttg gcttaagtca
     4561 ctggctgcct gactggcggc ttggctcagg ggcgcgcgtc ccaggttggt gtgcaaaagc
     4621 acgcccgtcg cgttgatgac gttttggagt tcgggcatgg attgcgctgc taggcgttgg
     4681 ttgacgcttt gggcaatctc agcgtgactg ggtaaagctt ttcccgcctt gagcgcgctg
     4741 cgtatttcat tcagcgtttc gcggatggcg gataccgtcc agtcgtgacc gtagctttgc
     4801 agggtggctt gaaaattatc tgattgaatc agttggtcaa cagatgggat ttggcgaaag
     4861 tcagccatgt ttagtcagcc tcttggtttg gtttccagcg gcttcgttcc caaaggcgca
     4921 ggaaaaactc aatcacgatc agcacgatta ggatgatgcc acccagcagg agcgtgagca
     4981 cgcgtcccat ttgcagccag gcgagcagac tgacaaagat accacccagg atggcttgcc
     5041 tgatgatgac aatgctttcg gccggcggat ttgacggaaa acgcaggttg ataaaccagg
     5101 ccaggggcag gaaaaatcca gaagcaaaga gcatcagaaa gaaaaagaaa agccagcgtg
     5161 ggcccagtgt gggcaaggtt tgggtgagta tgacagccat tccaccgcca ccgataatca
     5221 gcaatgcgaa aaaggccggc aaccacggga tgaaagaagt tcttttataa gctcgggtca
     5281 tgctgaaatt atactagatt ctgcctgatt cgttcactta agttagtgtt ttgtaaacta
     5341 tcgtttgctt atcattcttt agaatcttgc tggcttaaac aaaaaactgc ccgaaagcag
     5401 tttttgaagg cggggaggca gggattcgaa ccctgggccg aattctactc cggcaaccgc
     5461 ttagcaggcg gccccaatcg tccactctgg cacctcccca tatcatttgt taaggtcttt
     5521 tcagcggagg aagtgggatt cgaacccacg ttaggtttcc ctaaacctgt tttcaagaca
     5581 ggcgccttca accgctcggc catccctcca cagagcaagt attctaaca
//
LOCUS       DAOSFC010000063         5623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_12740_length_5623_cov_6.085848, whole genome shotgun sequence.
ACCESSION   DAOSFC010000063 DAOSFC010000000
VERSION     DAOSFC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12740_length_5623_cov_6.085848"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /gene="gap"
                     /locus_tag="PK791_02630"
     CDS             <1..933
                     /gene="gap"
                     /locus_tag="PK791_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOH92240.1"
                     /translation="MNAHLFKYDSSYGKCSCEVVVDENDDLIVDGHWIDVLQERDPNN
                     LPWKDLGVDLVIESTGVFTTARSDEKTGKTGADAHIKAGAKKVIITAPAKGEDITIVM
                     GVNDKKYDPELHHIVSNASCTTNALAPAAKIVNDNWGIVNAVMSTVHSYTNDQVLLDQ
                     GHKKDVRRGRAAALNIVPTSTGAAKAIALVIPELAGKFDGHSLRIPTPTVSIIDFVAL
                     LEKDTTIEEVNQAFIDAAEGEYKGIVGVVKGEYDDPLVSVDFRGDTRSSIVDLPNNML
                     MGTNLLKVVTWYDNEWGYSSRTVDLAKLIASKMA"
     gene            1032..2207
                     /locus_tag="PK791_02635"
     CDS             1032..2207
                     /locus_tag="PK791_02635"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOH92241.1"
                     /translation="MLNKKMLTDLDLQGKKVLVRVDFNVPLKDGVVQDDTRILAALPT
                     INYLLNNGAAVILASHLGRPKGKVNLEYSLKPVAVYLAKLLVAPVRFADDCRGSETKE
                     AAKTLQPGEVMVLENTRFYEGEEKNDPEMAKDLAELADLYVNDAFGTAHRAHASTAGV
                     TDYLPSAAGFLLEKEINYLGNAINDPKHPFVAILGGAKISDKIGVIENLLSKADKILI
                     GGGMANTFLAAKGYVMADSLVEAESLETAKELLAKSDGKLLLPVDVVIADAFDAEAKA
                     QTIAAGDVPEGWRVLDIGPETVKAFGAEIAKAGTVVWNGPMGVFEFPRFAEGTFGIAR
                     AVAECEGVTIIGGGDSVAAINQSGLAEKITHISTGGGASLEMLEGIELPGVAALDDK"
     gene            2291..2980
                     /locus_tag="PK791_02640"
     CDS             2291..2980
                     /locus_tag="PK791_02640"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOH92242.1"
                     /translation="MKKTKTTYALIILSVLVLLITACKPSQQEPVYTEEDLQRQVLET
                     QSARATQFAVETMAAQLTALSMPTFTPIPTEIPTQAPPPTQVPPTAVVNPVQPTATTV
                     PPTQDTGTKCYAMELVSETIPAGTTMTPGEKFEKSWTIRNTGTCEWSREFDVYLSGGE
                     AYGSNKYGDFKENVPPGETTTVTLYDLVAPQTPGTYYSYWVVMTVDGIRFGYGPNGDW
                     GLSIQIEVRKP"
     gene            3086..4339
                     /locus_tag="PK791_02645"
     CDS             3086..4339
                     /locus_tag="PK791_02645"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOH92243.1"
                     /translation="MKKQTFFKTNWIVAVAVIVISVVALAACNLPGSEKPLGDPIEQA
                     KQTLIAQQTQEHFQTLVAQLTATVPVESTEPVEPVEPTESPVVQPSNTVEPTAVPATS
                     VPPTAVPPTAVPPTAVPTRPAPTATPIPCYQLSFVADVTIPDGTKIPGGNTFTKTWRI
                     KNTGSCTWDERFDVAFVRGTQLAASAIYDFRKVVKPGETVEISIEMTAPTTNGVYTSE
                     WQMVNPNGVKFGGGPKSDGTFWAKIEVVDGKGAIYNFATSACDADWSSDLNSKLPCPG
                     DRKKAGQGYVIYEKNPIREDGGKENDPGLITRPSPTVSGGYIQGIYPAINIKSGDEFR
                     ALIQCEGGAEKCSLKFELYYKIGDGKFIELGEWIEVYEKKWNTISIDLSALAGNKVTF
                     SLVVWNHGTAEDNIGLWLDPIIYRP"
     gene            4532..5302
                     /gene="tpiA"
                     /locus_tag="PK791_02650"
     CDS             4532..5302
                     /gene="tpiA"
                     /locus_tag="PK791_02650"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOH92244.1"
                     /translation="MTARKPMVAANWKMNKTAFEARELTQAMLPELKKIEAVDIVLCP
                     PFTNLSLVCEQIAETDLKLGAQNMHWQEKGAFTGEISASMLADFCDYVILGHSERRQM
                     FGETNETVNKKAHTALAHDLKPIICVGESLEQNQAGETAQVVETQCRESLQDITPEQA
                     QNIVIAYEPVWAIGTGLAATPEGANEVHRDVIRPVLRDLFGAEIAEGMRILYGGSVNA
                     ENAGELFAMSDIDGGLIGGASLKADAFVAVVKAAAETL"
     gene            5407..>5623
                     /locus_tag="PK791_02655"
     CDS             5407..>5623
                     /locus_tag="PK791_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92245.1"
                     /translation="MLEKLETLRHEAEADLAGITDMEALQKVKHDWLSKNGKLTDILK
                     AIPTLPAEQRRPFGIGANEIKTMLTEKL"
BASE COUNT         1524 a         1252 c         1507 g         1340 t
ORIGIN      
        1 atgaacgcgc atctctttaa atacgactca agctacggaa agtgcagctg tgaggttgtg
       61 gtggacgaaa acgatgacct gattgtggat ggccattgga ttgacgtttt gcaggaacgt
      121 gaccccaaca atttgccctg gaaagatctt ggcgttgatt tggtcatcga atccacgggc
      181 gtctttacca ccgcgcgcag tgatgagaaa actggcaaaa caggcgcgga cgcgcatatc
      241 aaagctggtg cgaagaaagt tatcatcacc gcgccagcca aaggcgaaga tatcaccatc
      301 gtgatgggtg tgaacgataa aaaatacgac cctgaactgc atcacattgt ctcgaatgct
      361 tcatgcacca ccaacgcatt ggcgccggcg gctaagattg tcaacgacaa ctggggcatt
      421 gtaaacgcgg tgatgagtac cgtgcacagt tatacgaacg accaggtgct actggaccag
      481 ggtcataaaa aggacgtgcg tcgtgggcgg gctgcggctc tgaacattgt gcccacttca
      541 accggcgcgg ccaaggcgat tgcgcttgtt attcccgaac tggctggcaa gtttgatggc
      601 cactctctgc gcataccaac cccaacggtt tcgattattg actttgtggc tttgcttgaa
      661 aaagacacga cgattgaaga agttaatcag gcttttattg acgccgctga aggcgaatat
      721 aagggcattg tgggtgtggt caagggcgag tatgacgacc ctttggtctc ggtggacttc
      781 cgcggtgata cacgcagttc aattgttgac ctgcccaaca atatgctgat gggcaccaac
      841 ttgctgaaag tggtgacctg gtatgacaat gagtggggct attccagccg cacagttgat
      901 ttggcgaagc ttattgccag caagatggca taagatatga gaatgtctgg cactaataag
      961 tgcacggcac tctgttgtaa cagtaaaaag tgataatata acgatataaa caaagcgttt
     1021 ttggaggaat aatgctcaat aagaaaatgt taactgattt agacctgcaa ggcaaaaaag
     1081 ttttggtacg ggttgacttt aatgtgcccc taaaagatgg cgttgtgcaa gatgacacac
     1141 gcattttggc tgctctgccc acaatcaatt atttgctgaa taatggtgcg gcggttattt
     1201 tggcttcgca tctgggacgc cccaagggaa aagtgaacct ggaatacagc ttgaaacccg
     1261 tcgcggttta tctggcgaaa cttttggtcg cgccggtgcg ttttgcggat gattgccgtg
     1321 gctcagagac caaagaagcg gccaaaacct tgcaacccgg cgaggtgatg gtgctggaga
     1381 acacccgctt ttacgaaggc gaggaaaaga acgaccccga aatggctaaa gacctggcag
     1441 agttggcaga cctttatgtg aacgacgcct ttggtaccgc tcatcgcgcg catgcctcaa
     1501 ccgcgggcgt gacagactat ttgccctccg cggctggctt tttgctggaa aaggaaatta
     1561 actatttggg taacgccatc aatgacccca aacatccctt tgtggcgatt ttgggtggcg
     1621 cgaaaatcag cgataaaatc ggcgtgattg aaaatctgct cagcaaagcc gacaaaatcc
     1681 tgattggcgg tggcatggcg aatacctttt tggctgccaa aggatacgta atggcggatt
     1741 cattggtaga agctgaatca cttgaaaccg caaaagaatt gctggcgaag agtgatggca
     1801 aactgctgtt gcctgtggat gtagttatcg cggacgcttt tgatgctgaa gccaaggcgc
     1861 aaaccatcgc ggctggtgat gttcctgaag gctggcgcgt tttggacatt ggccctgaaa
     1921 cggtcaaagc ttttggcgcg gaaattgcca aagctggtac agtggtttgg aatggtccca
     1981 tgggtgtctt tgagttcccg cgttttgctg aaggcacttt tggtatcgcg cgcgccgtgg
     2041 cagaatgcga aggcgtgacc atcatcggcg gtggcgattc ggtggcggca attaaccaaa
     2101 gcggtttggc ggaaaaaatt acgcatatct caaccggtgg tggtgcgtca ctggaaatgc
     2161 tggaaggtat cgaactcccg ggtgtagctg ccctggatga taagtaaact tgcatttctt
     2221 aaagcgtgat agaattaagc cagtgtcttg aaggaacact tttgaaacta tgcgttaaat
     2281 cggagagatt atgaaaaaga caaaaactac atacgcactc attatattaa gcgttttggt
     2341 tttgttgatt acggcttgta aaccgagtca acaggaacca gtttacactg aagaagattt
     2401 acaacgacag gtgcttgaaa cccagagcgc aagagcgaca cagttcgcgg ttgaaacgat
     2461 ggcagcccag ctgaccgctt tgagtatgcc aacgtttacc cccatcccaa ctgaaatccc
     2521 aacccaggcg cccccaccca cgcaggtacc ccctacggca gtagttaacc cagtacagcc
     2581 gactgcgaca acggtaccgc ctacccagga tacaggtaca aaatgttatg cgatggagct
     2641 tgtatctgaa acaattcccg caggtacgac catgacccca ggtgagaaat tcgaaaaatc
     2701 ctggacaatc agaaacactg gtacctgtga atggtccaga gaatttgacg tctatttgag
     2761 tggtggtgaa gcctacggtt cgaataagta tggtgatttt aaggaaaatg tcccccccgg
     2821 ggagacgaca acagtcacgc tttatgatct ggtggcacca caaacaccag gaacctatta
     2881 cagttattgg gttgtgatga cagttgatgg aatcagattt ggttatgggc caaacggaga
     2941 ttgggggctg tccatccaga ttgaagtgcg caagccgtaa aaaagatgaa agccacctaa
     3001 atgggtggct ttttttggaa cttttttcgc ttgggatacg tcttatatgt aaagtctgga
     3061 ttattaagac aaataggagc ttaagatgaa gaagcaaact tttttcaaaa cgaattggat
     3121 tgtcgcagtc gctgtgattg tgatatccgt tgttgccctt gcggcttgta atttacctgg
     3181 ttccgagaaa cctttggggg acccgattga acaagccaag caaacgttaa tcgcgcagca
     3241 aacgcaagag cattttcaaa ccctggtggc acagttgaca gctaccgtgc ctgtcgaaag
     3301 caccgagcct gtcgagccag ttgaaccgac agaaagcccg gttgtgcaac ccagcaatac
     3361 cgtcgaaccg accgcagtgc ctgctaccag tgtgccccca acagcagtgc caccgacggc
     3421 agtacctcca actgctgtgc caacgcgtcc tgcacccacg gcaacaccga taccttgcta
     3481 tcagctgagt tttgtggcgg atgtaaccat ccctgatgga acgaagattc ccggtggaaa
     3541 tacctttacc aagacctggc gcattaagaa cacgggttct tgtacctggg atgaacgctt
     3601 tgatgtcgct tttgtaagag gcacgcagct ggcagcgtcg gcaatttatg atttccgtaa
     3661 agttgtaaaa cctggcgaaa ccgtggaaat ctccattgaa atgaccgcgc ccacgactaa
     3721 tggcgtttat acgtctgagt ggcagatggt aaatcctaat ggcgtgaagt ttggcggtgg
     3781 gccaaagagt gatggcactt tttgggcgaa gattgaagtt gtggatggca aaggcgcgat
     3841 ttataacttc gccacttctg cctgcgatgc cgattggtcg agtgatttga acagcaaact
     3901 tccttgcccg ggtgaccgga aaaaggcggg tcaaggttat gtcatttacg agaaaaaccc
     3961 cattcgcgaa gatggtggta aagagaacga cccaggattg attacacgac ccagcccaac
     4021 agtgagtggt ggttatatcc agggcattta tcccgcgatc aacattaaat ctggcgatga
     4081 gttccgggct ttgattcaat gtgagggcgg tgctgagaag tgcagtttga aatttgagtt
     4141 gtactataaa attggcgatg gtaagttcat cgaactgggc gaatggattg aggtctatga
     4201 aaagaaatgg aataccattt ctatcgacct tagcgcattg gctggaaaca aagtcacctt
     4261 ctcgctggtg gtatggaacc atggcacagc cgaggacaat atcggacttt ggcttgaccc
     4321 gattatttac cgtccgtaaa atgagatgat tttttgaaaa agttaaaacc gtgccacgcg
     4381 cacggttttt tctatatcgc gcaaggctat aggggcttgt tcccgttttg gcacttatcc
     4441 tggaaatcta tacacatatt aaagaattat gcgttatatt cataccttgg cgtgataaaa
     4501 tgaaagaaaa caaatcatcc tgaggtgaaa aatgactgca agaaaaccta tggttgctgc
     4561 taattggaaa atgaacaaga cagcttttga agcgcgtgag cttacccagg cgatgctgcc
     4621 tgaactaaag aaaattgagg ctgtggacat tgtgctttgt ccgcctttta caaatttgag
     4681 tctggtatgt gagcaaattg ccgaaaccga cctaaaactt ggcgcgcaaa atatgcactg
     4741 gcaggaaaaa ggcgctttca ctggcgagat ttccgcatca atgctggcgg atttttgtga
     4801 ttatgtcata ttagggcaca gtgaacgacg ccaaatgttt ggcgaaacga acgaaaccgt
     4861 gaacaagaaa gcgcataccg ccctggcgca tgatctgaaa cccatcatct gcgtgggtga
     4921 aagccttgag caaaaccagg ccggcgagac cgcgcaagtg gttgaaacac agtgccggga
     4981 aagtttgcag gacatcacgc ccgagcaagc ccaaaacatc gtcatcgcct atgaaccagt
     5041 ctgggcgatt ggtaccgggc tggccgcgac gccggaaggc gcgaacgaag tgcatcgcga
     5101 cgttattcgc cccgtattgc gcgatttgtt tggcgctgag attgctgaag gcatgcgcat
     5161 tttgtacggt ggttcggtga atgccgaaaa cgccggcgaa ttgtttgcta tgtcggatat
     5221 tgacggcggt ttgattggtg gcgcgagcct gaaagcggat gcttttgtgg ctgtggtgaa
     5281 agctgccgct gaaaccctgt agttgccatc tgaaagcgga ataattgact tgactccgcc
     5341 cgcgaaaaac ctataatttt cgggcgtgaa ctgggcaaaa agcccaagtg tttgttagga
     5401 gattttatgt tagaaaaact tgaaaccttg cgacatgaag ctgaagctga tttggcaggc
     5461 attactgaca tggaagcttt gcaaaaagta aagcacgact ggttgagcaa aaacggcaag
     5521 ctgaccgata tcctcaaagc cattccaacc ttgccggctg agcaacgccg tccgtttggc
     5581 atcggcgcga acgaaatcaa aaccatgctt acggaaaaac tgg
//
LOCUS       DAOSFC010000064         5604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_12797_length_5604_cov_5.360786, whole genome shotgun sequence.
ACCESSION   DAOSFC010000064 DAOSFC010000000
VERSION     DAOSFC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12797_length_5604_cov_5.360786"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PK791_02660"
     CDS             complement(<1..472)
                     /locus_tag="PK791_02660"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOH92246.1"
                     /translation="MPDKKDRLDSIREELRHCRQCALARGRRNVVPGEGPHHVQVMFI
                     GEGPGRNEDQQGKPFVGQAGAVLDELLAAAGLKREEVFIANVIKCRPPNNRDPLPEEI
                     SACRHWLDEQINLLDPKIIVTLGRFSMARFIENARIGRIHGQAFEIEGRKVVTMY"
     gene            complement(514..1038)
                     /gene="rimI"
                     /locus_tag="PK791_02665"
     CDS             complement(514..1038)
                     /gene="rimI"
                     /locus_tag="PK791_02665"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOH92247.1"
                     /translation="MSQNSKTEDIIVRKMVHADLAQVEAHEKANFSQPWPSGSFAYEL
                     RQDSYSIPLVAVDTTQAENANIIGVLVAWRLADSLEIATISVAKAYRHLGIARSLLAQ
                     AICMSEEHGITEVILEVRASNRDALRLYLGLGFEVVGIRPGYYQDNNEDALLLTLKPI
                     DFEKIGNLIGLNYV"
     gene            complement(1035..1706)
                     /gene="tsaB"
                     /locus_tag="PK791_02670"
     CDS             complement(1035..1706)
                     /gene="tsaB"
                     /locus_tag="PK791_02670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOH92248.1"
                     /translation="MLLAIDTSTDWTGIALFDGTQILAEQIWRGKHYQSVELVPAIQR
                     MFSKSNVTPAQLSGVGVATGPGSFTGLRIGMSVAKGFALACKLPVVGIPSLDVLCAAQ
                     PGLRRPMIAMIELGRGRFAWIRYVYKNRAWQAEHDIQLGDAKAIAATIKSPIYVVGDL
                     NAETRQILGRKWKTTQLASAAACVRRPAVLAELAWQKIKAGQEDDPALLSPIYVHTLS
                     NVPNV"
     gene            complement(1709..2209)
                     /gene="tsaE"
                     /locus_tag="PK791_02675"
     CDS             complement(1709..2209)
                     /gene="tsaE"
                     /locus_tag="PK791_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOH92249.1"
                     /translation="MAILKPNAFEFFSHSAEQTRRLGVRLGAMLPVGTVLCLEGDLGS
                     GKTTLVQGIAQGWGSTDRVTSPTFVILNQYRRPNGMQLFHADAYRLAENDPFDLAILD
                     LEQALENGVLIVEWADRLRDSLPKEHIWIEMQWMGPNSRHFILTPEGKQNTQILKDLQ
                     LAIYGV"
     gene            2411..3481
                     /gene="ychF"
                     /locus_tag="PK791_02680"
     CDS             2411..3481
                     /gene="ychF"
                     /locus_tag="PK791_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOH92250.1"
                     /translation="MQLGIIGLPQSGKTTLFNALTNANIPTDNLGGHMQVHTAVVDVP
                     DPRVDELCAIYKPKKTVHAKVTYADIAGIDGKAGKAGLPGPLINQMASMDGFLHVVRQ
                     FESPVVAHQEGSVDPLRDIESMDTEFILNDLVMIERKLERLTEEHRRGVSRPREEIQK
                     EIDVFKSLQAVLNENLPLRHHKFTPEEDETLASYGFLSRKPQIIVFNQSEGQTPPDYK
                     SPHPDTFVIALPAKLEMDIAALEPEDALIFMEEYGIAELAMSRLIKLSYDLLGVHSFF
                     TAGEKEVHAWTIKRGTNAREAAGAIHSDMEKGFIRAEVISYQDLMHFGSTAEARNKGR
                     LRVEGKSYIVEDGDIIEIRFNV"
     gene            3686..4435
                     /locus_tag="PK791_02685"
     CDS             3686..4435
                     /locus_tag="PK791_02685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92251.1"
                     /translation="MKNINQPTDLQKAAERGEPSHVWREGQDRRLKMILEAAEDRVNG
                     TVFVDGCGVGMYLAHLAPMAETILGMDIEFPRVQESKVYTPKVFTGAGEYLALPSNSI
                     DLLLSHEVLEHVQNDKQAIQEMVRVLKPGGRLVLFCPNRGYPFETHGIYKNGKYIFGN
                     IPLVNWLPRKWRDKFCPHVEIYTRQDLRKLFKGLPVKFITQRTIFGGYDNIIQSRPRL
                     GKFLRRVLYALEKTPLQRLGLSHFWVVEKQN"
     gene            4542..5159
                     /gene="coaE"
                     /locus_tag="PK791_02690"
     CDS             4542..5159
                     /gene="coaE"
                     /locus_tag="PK791_02690"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOH92252.1"
                     /translation="MQDKVFILGLTGNIASGKSYVLAYLAKRGAKTIDADLVAQQSYL
                     PGKPAYNALIAHFGSDIVRADGQIDRTALGKIVFADPAQLKALEDIVHPATTNAITAL
                     VNEAQTTKSTNLIVVEAIKLFESGMAQKYDATWVTVADDETRLNRLIQGRGMDPISAA
                     KRLRSQSPQEEKAKLADFVIHTDGSFEETDAQIETGLEKLGLRLS"
     gene            5199..>5604
                     /locus_tag="PK791_02695"
     CDS             5199..>5604
                     /locus_tag="PK791_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92253.1"
                     /translation="MARISLTGIKPTGSPHIGNYFGMYKQAIALSGSYLGLYFVADFH
                     ALTTTPNPKDLQRDIYEVAAAWLALGLDPDKSIFFLQSDVPEIPECSWILACSTAKGL
                     LNRAHAYKAAIDKNLEIDRDPDYGVNAGLYNYP"
BASE COUNT         1456 a         1515 c         1295 g         1338 t
ORIGIN      
        1 gatacatggt aaccactttt cggccttcaa tttcaaaggc ttgcccgtga atccgcccaa
       61 tgcgcgcgtt ttcgataaag cgcgccatcg aaaaacgtcc cagggtcaca atgattttcg
      121 ggtctaaaag gtttatttgc tcgtcaagcc agtggcgaca ggcgcttatt tcctcgggta
      181 aggggtcgcg gttgttgggc ggacgacatt tgatgacatt cgcgatgaat acctcttcgc
      241 gttttagtcc agccgcagcc agcaattcat ccaacaccgc gccagcctgc cccacgaagg
      301 gcttgccttg ttgatcttcg ttccgtccgg gaccttcgcc gataaacatc acttgaacgt
      361 gatgcgggcc ttcacccggc acaacgttac gtcgccctct agccaatgca cattgccggc
      421 agtgacgcaa ttcttcacgt atactatcca aacggtcttt tttatcaggc attaaatgct
      481 ccagtaatgg attctacacc attgtccact atattagacg taattcaaac cgattaagtt
      541 ccctattttc tcaaaatcga ttggttttaa ggttaaaagc aaagcgtctt cattattgtc
      601 ctgataatag cccggacgaa tgccgaccac ttcaaatcca agacccagat aaagacgcag
      661 cgcgtcacgg ttactcgcgc ggacttccaa aatcacttct gtaatgccat gctcttcgga
      721 catgcaaatt gcctgtgcca aaagactgcg cgctatcccc aaatggcgat atgccttagc
      781 gactgaaatg gtggcaattt cgagactgtc cgcaagacgc caggccacaa gcacgccgat
      841 aatattcgca ttttccgcct gggtggtatc aacagcaacc aaggggatac tgtaagaatc
      901 ctggcgcagc tcataggcaa acgaaccact cggccagggc tgactaaagt tggctttttc
      961 gtgcgcttca acctgtgcca gatcagcatg caccattttt ctgacaatga tgtcttcagt
     1021 cttggagttt tgactcatac attagggaca ttgctaagcg tatgcacata gattggcgaa
     1081 agaagggcgg gatcgtcttc ctgacccgct ttgattttct gccatgccag ttcagccaaa
     1141 accgctggcc ggcgcacaca tgcagccgcg ctcgccagtt gcgtcgtttt ccatttacgc
     1201 cccaggattt gtcgcgtttc agcgttcagg tcccccacaa cataaatcgg cgatttgata
     1261 gtagctgcga tggctttggc atcgcccagc tgaatatcat gctcagcttg ccaggcgcgg
     1321 tttttataga cataacgaat ccaggcaaaa cgccccctgc ccagttcaat catcgctatc
     1381 atggggcgtc ttagtccagg ctgagcagcg caaagcacat ccaaagaagg aatacccacg
     1441 acaggcaact tgcatgccag cgcgaagcct ttggcaacgc tcataccaat gcgcaagccg
     1501 gtgaatgaac ccgggccagt agccacgcca acaccgctaa gctgcgctgg cgtaacgttc
     1561 gatttgctaa acattctttg aattgccggc acaagttcca cactctgata atgttttcca
     1621 cgccagattt gctcggcaag gatttgcgtg ccatcaaaaa gtgcgatgcc ggtccagtct
     1681 gttgatgtat caattgctaa taacatactt acactccgta tatcgcaagt tgcagatctt
     1741 ttaggatttg cgtattctgc ttgccttcgg gggtcaagat aaaatgacgt gaattaggcc
     1801 ccatccattg catttcaatc caaatgtgct ctttgggcag agagtccctg agtcggtccg
     1861 cccattccac aatcaacact ccattttcaa gggcctgttc aaggtcgaga atggccaaat
     1921 caaacgggtc gttctcagcc aggcgataag cgtcggcgtg aaaaagctgc ataccgttcg
     1981 gacggcggta ttgattgaga atgacaaaag taggactggt aacacggtca gttgagcccc
     2041 agccctgggc gataccctgc accagtgtgg ttttacccga acccaaatcc ccttcaaggc
     2101 aaagaaccgt gcccaccggc aacatcgcgc ccaaacgcac gcccaggcga cgggtttgct
     2161 cggcactgtg tgaaaagaac tcgaaagcat tcggtttaag gatagccata ttcttcttta
     2221 tttcctctgg gtaaattata ccttatatga aaatgtttcc gaaaaattcg gtggctcatt
     2281 tcatgatttg ctggatgttt cgcgtagaac ttgcttgttt ctctttttcg cgtctgtgcc
     2341 tgcgctgggg tataatcgaa acattatgat tgaactttag cagcctggtc tgctgacgaa
     2401 ttggagttac atgcaacttg gaattatagg actgccccaa agtggcaaga caacactttt
     2461 taatgcctta accaacgcca atatccccac tgataacctc ggtggacata tgcaagtcca
     2521 taccgctgtg gtggacgtgc ccgacccgcg tgttgacgaa ctttgcgcca tttacaaacc
     2581 caagaaaacc gtccacgcca aagtcaccta tgccgatatc gccggaatcg acggcaaagc
     2641 tggaaaagcg ggtctgcctg ggccacttat caaccagatg gcatcgatgg acggtttttt
     2701 gcacgtcgtg cgtcagttcg aaagccctgt ggtcgcccac caggaaggca gcgttgatcc
     2761 tttacgtgac attgaaagca tggacaccga attcattctc aacgacctgg taatgattga
     2821 acgcaaactt gaaagactga ccgaagaaca tcgtcgcggt gtcagccgtc cgcgtgaaga
     2881 aattcaaaaa gaaattgacg tcttcaaatc attgcaagct gttttaaatg aaaatctgcc
     2941 cttgcgccat cataaattca ctccggaaga ggacgaaact cttgccagct atggcttttt
     3001 aagccgaaag ccacaaatca tcgtcttcaa ccagtccgaa gggcaaaccc caccggatta
     3061 caaaagcccc cacccagaca cctttgtgat tgccctgccc gccaagctcg aaatggacat
     3121 cgccgcgctt gaaccggaag acgcgcttat ctttatggaa gaatatggca tcgctgaact
     3181 ggctatgtcg cgcctcatca agctttcgta tgaccttttg ggcgtgcaca gcttctttac
     3241 cgccggcgaa aaggaagtcc acgcctggac gataaaacga ggcaccaacg cccgcgaagc
     3301 cgccggagcc attcactcgg atatggaaaa gggcttcatc cgcgcggaag tgatttcgta
     3361 ccaagatttg atgcattttg gcagtactgc cgaagcccgc aataaaggcc gtctgagagt
     3421 agaaggcaaa agctacatcg ttgaagacgg tgatatcatc gagataaggt ttaatgttta
     3481 gaatgtctgc caggattctg cgctccataa aaattacagc cttctcttcg aatataagct
     3541 catttcccct tctcctgtgt catttaattt cttccgacct gtggcacaca cttccccccc
     3601 tccaaaggag ggggattaaa gggggtgggg cttttcaaaa acaccaacaa agcgcgtcga
     3661 acccaaataa tttcaggagt atcctgtgaa aaacattaac caacccactg atctgcaaaa
     3721 agccgccgaa cgtggtgaac catcgcacgt ttggcgcgag ggccaggacc ggcgcctgaa
     3781 gatgattctc gaagctgctg aagaccgcgt taacggaacc gtcttcgttg atggttgtgg
     3841 cgtgggtatg tatctcgcgc atctcgcgcc catggcggaa acaattcttg gaatggatat
     3901 cgaatttccg cgtgtgcagg aaagcaaagt ctacacgccc aaggtcttta ccggcgcagg
     3961 cgagtatttg gcattgccca gcaacagcat tgaccttttg cttagccatg aagttctcga
     4021 acacgtgcag aacgataaac aagccattca agagatggtg cgcgtactca agcccggtgg
     4081 acgccttgtg cttttctgcc ccaaccgcgg ttaccccttt gaaacgcacg gcatctacaa
     4141 aaacggtaaa tacatatttg gcaatatccc gctggtcaac tggttgcccc gcaaatggcg
     4201 cgataaattc tgcccccatg ttgaaatcta cacccgccag gacttgcgca aactgttcaa
     4261 aggcttgccg gtaaagttca tcacccaacg cacaatcttt ggcggttacg acaacatcat
     4321 ccaaagccga ccccgattag gtaaattttt gcggcgtgtc ctttacgcgc tcgaaaaaac
     4381 gcctttgcag cgtttaggcc tgtcgcactt ttgggtagtt gaaaagcaaa actaagcccg
     4441 attccagcgt agactaaatc gtcagcttgg ttgcacaagg ttttccataa acatggcaaa
     4501 tgctgttatc acgcccatca cacttaaagt atacttagct tatgcaagat aaagttttca
     4561 tccttggctt aacaggcaac atcgcttcgg gcaagtctta cgttttggct tatctcgcca
     4621 aacgtggcgc gaaaaccatt gacgctgacc tggtcgccca gcaaagctac ttgcccggca
     4681 agcccgctta taacgcgctc atcgcgcatt ttggcagcga tattgtacga gccgatggcc
     4741 aaatcgaccg aaccgcgctg ggcaaaatcg tttttgcgga ccccgcccag ctgaaagcgc
     4801 ttgaagatat cgtacacccc gcgacaacga acgctatcac cgcgctcgta aacgaagccc
     4861 agacaacaaa aagcacaaac ttgattgttg ttgaagcgat caaacttttt gaatccggca
     4921 tggctcaaaa atatgacgcc acctgggtaa ccgttgcgga tgacgaaacc cgcctaaacc
     4981 gtcttattca gggccgtggc atggacccca tcagcgccgc aaaacgcctt aggtcacaaa
     5041 gtccgcagga agaaaaggca aagctggctg attttgttat ccacaccgac ggcagctttg
     5101 aagaaactga cgcacaaatt gaaaccgggc tcgaaaaatt agggctcagg ctaagttaat
     5161 tcgcttataa tagacccact aagattttgg agattaaaat ggcacgtatt tcactcacag
     5221 gcatcaaacc gaccggcagt ccgcacatcg gcaattattt tggcatgtac aagcaagcca
     5281 tcgccctatc gggaagctac ctggggcttt atttcgttgc tgattttcac gccctgacta
     5341 ccacacccaa ccccaaagac ctgcaacggg acatttacga agttgctgcc gcctggcttg
     5401 ccttgggact cgacccggat aaaagtattt tctttttgca gtccgacgtt cccgaaatcc
     5461 ccgaatgctc ctggatttta gcctgctcta ccgccaaggg tttgctcaac agggcgcacg
     5521 cctataaagc cgccatcgac aaaaaccttg aaatcgaccg cgacccagac tatggtgtta
     5581 atgctggttt gtataactat cccg
//
LOCUS       DAOSFC010000065         5604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_12800_length_5604_cov_4.051180, whole genome shotgun sequence.
ACCESSION   DAOSFC010000065 DAOSFC010000000
VERSION     DAOSFC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12800_length_5604_cov_4.051180"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="PK791_02700"
     CDS             <1..1484
                     /locus_tag="PK791_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92254.1"
                     /translation="FQSAHDPEAERLMDQAEEAFYKGNYQVAIPLYEKVIQMEPSWAR
                     AQEHHAEAEEYLRSGNIPSVALPPEAGKAYGKAQSAARVFRYQIALNYLDDAFDHLEE
                     AGIKRWREGEDLRADLENQMQAYDVYKEGLNLLSLGDLQAAFSKVQTAASAVAIPEYI
                     DKANEIRADISAINEISDIVSMSGKIPPAMLADAKGKLERIRMKYGEIEQIRRLRTRL
                     DLMIPAMIQNLLDNTQRLKQEAADAPTATIAKQKVTATRENLDLLRQLEAYDAESLNL
                     EDEISRLETEIHTNLDAIGRAKEAFESGNKFFALESWKISRNARKRFPQDPDVLKLKR
                     NYYPSYAVGILGILVISAILIVTVSVGIRRISAGIHARNLAKTPTVTSTPTITLTSTP
                     TLTPEPTLTPTPYLSPTPTITLTPTPIMYAKTIRELYVRSDCYENFRSVARVPAGTRL
                     TLLPMTERRFDSFNRECLLVDFKSGEFAFIGYVLLQDLEPFTE"
     gene            1559..2908
                     /locus_tag="PK791_02705"
     CDS             1559..2908
                     /locus_tag="PK791_02705"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOH92255.1"
                     /translation="MKDLETRYQETLDWIYSWIDFSMSRHVDESHRFFKLDRMNRLMA
                     LLDWPNRHYPCVHVAGTKGKGSTASFIASALRACGYKTGLYTSPHLEDFRERIQINGE
                     QISVSELVDLADQMRPRTEIVADVTTFELTTAIAFQYFANQQIDIAVLEVGLGGRLDA
                     TNIVDPLVSVITPISFDHMNVLGNTLTEIAQEKAGIIKPNRPVVIAPQHEEAEKEILR
                     VAASQNAPVTLADKAYTWQILSHDINHQLIAIQQTDTNQQNPEQLTPVELDIPLLGRH
                     QADNAATSLATLEILRQSGFNITQDDLKHGFQDVYWQTRFEVLRKEPPFIADAAHNGD
                     SMQKLRQALDDYFPGLPFILLFGASADKSLTTMLDIILPRVEAVVCTQSIHPRAADAF
                     ELQKLIEPYGIPVYAEVPAEAGLAKALELAGDSKGIVATGSLFLAAAARAIWHQDYQ"
     gene            2921..5434
                     /gene="lon"
                     /locus_tag="PK791_02710"
     CDS             2921..5434
                     /gene="lon"
                     /locus_tag="PK791_02710"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOH92256.1"
                     /translation="MDSNTPKFPFQATDRIDFSNLVNLHDVPERRPGKDGTFTAPAII
                     ITKSPILPDSLSTMTVATDALILSAINQAWEENTTIVGVFDDGKKEQSFLSLGVEFAP
                     GEPIQTDKDTVTFIVQGRRRVEVLEIFVDENDMLFVKAALIKIPKRRSKEVQALMRQV
                     IKYFETFAQLSQHLPPTVIQFARQLEDPGELADIVTASINLKPAQMMEIITAVDPAER
                     IETALRLIKHEVSLLELEAEINDRVQQELDQGQREIYLREKIEKLQQELNDGKSTDPD
                     IRSLEDLLESKEYPEEVVKTAKKELERLKMTPSLSPESGMISTYLHWLLELPWNEETQ
                     DRLDIKEAQAILDKHHYGLPKAKDRILEYLAVRSLNPPKNRQPILCFVGPPGTGKTSL
                     GKSIAEAMGRKFVRRSLGGVRDESEIRGHRRTYIGSMPGRILETISKVGVANPLFMLD
                     EIDKLSSDIHGDPASALLEVLDPEQNYSFSDHYLEVPFDLSKVFFITTANTVQSIPPA
                     LLDRMEIIEFPGYIEEEKLEIARQFLIPEQLEENGLQDKSIEFTEDALRLIIKSYTYE
                     AGVRNLERMIGAVLRKTARKLAEGEAIEKIITPELVEERLGPVEFFPITAELQDEVGM
                     ATAVAWTENGGEIMQIEVLVMPGKGNLQITGQVGEIMQESAQAALSYIKSKAENFQIP
                     DSLFEETDVHIHVPEGSIPKDGPSAGITMAIAMTSAVLGVPTRHEVAMTGEITLRGRI
                     LPVGGVREKVLAAHRAGITTILLPTKNQKDLIEVPAPVLEQLRIVFVEHMDEVLKEAL
                     AGEAIYAKKPKETPPRHPRPTKNKMAQRSQQNSDTQAVA"
BASE COUNT         1573 a         1588 c         1251 g         1192 t
ORIGIN      
        1 tcttccagtc ggcccatgac cccgaagcag aacgactgat ggaccaggcc gaagaagcct
       61 tttacaaagg caattatcaa gtcgccattc ccttgtacga aaaggttatc caaatggaac
      121 cttcctgggc acgcgcccag gagcaccatg ctgaggctga agaatacctg cgttctggta
      181 atatccccag cgtcgctttg ccccctgaag ccggtaaagc ttacggtaaa gcgcaaagtg
      241 cggcgcgtgt attccgctat caaatcgcgc ttaattacct cgatgatgcc tttgaccatc
      301 ttgaagaagc cggtattaaa cgctggcgcg aaggtgagga cttacgcgct gaccttgaaa
      361 accaaatgca agcttacgac gtctacaagg aaggtcttaa tcttctcagt ttgggcgatt
      421 tacaagccgc gttcagtaaa gttcaaaccg ccgccagtgc agttgcaatc cccgaataca
      481 tcgacaaagc caacgaaatt cgcgcggata tcagcgcgat aaacgaaatc agcgacatcg
      541 ttagtatgag tggcaaaatt ccgcctgcta tgttggctga cgcgaaaggt aagctggaac
      601 gtattcgcat gaagtatggt gaaattgaac aaatcagacg gttgcgcaca cgcctggatt
      661 tgatgattcc ggcgatgata caaaacctgc tagataacac ccaacgtctg aaacaggaag
      721 ctgctgacgc gccaaccgcg accattgcca aacaaaaagt cactgccacc cgcgaaaatc
      781 ttgacctttt gcgccagctt gaagcctacg atgcggaaag ccttaacctt gaagacgaaa
      841 ttagccgcct agaaactgaa atccacacaa atctagatgc catcggacgc gccaaagaag
      901 cttttgaaag tggtaacaag tttttcgcat tggaatcctg gaaaataagt cgcaatgccc
      961 gcaaacgctt cccgcaagac cctgatgtac tgaagctaaa acgaaattat tacccgagtt
     1021 atgcggttgg cattttaggc atcctggtta tcagcgctat actcatcgtc acagtaagcg
     1081 ttggcatccg ccgtatcagc gcaggcatcc acgcgcgcaa ccttgccaaa acacccacgg
     1141 tgacaagcac gccgactatc acgcttactt caacgccaac actaacccct gagccaacgt
     1201 taacgccaac gccttatcta tcacccacac caaccataac gctaacccca actcctatca
     1261 tgtatgcaaa aaccatccgc gaactatacg tgcgtagtga ctgttatgaa aatttccgat
     1321 cggttgcgcg tgttccagct ggcacccgcc tgacacttct gccgatgacc gagcgccgat
     1381 tcgacagctt caacagggaa tgcttactgg ttgacttcaa aagtggtgaa tttgccttta
     1441 ttggctatgt actcttgcaa gacttggaac ctttcaccga ataaacgctt tcaaaccgaa
     1501 taatatcgcg ctttgcaaga aaagcccatc aagccatatt aagagttaag attagaccat
     1561 gaaagattta gaaacacgtt accaagaaac cctggactgg atttacagct ggattgactt
     1621 tagcatgagt cgccatgtgg acgagtcgca ccgctttttt aaactcgacc gcatgaaccg
     1681 tttgatggca ctcctggact ggcccaaccg acattaccct tgcgttcatg tcgccggcac
     1741 taaaggcaag gggtctaccg cctcctttat cgcctccgcc ctgcgcgctt gcggctataa
     1801 aaccggactt tacacctctc cacatcttga agatttccgc gagcgaattc aaatcaatgg
     1861 cgagcaaatt tcagtcagcg aacttgttga ccttgccgac caaatgcgcc cgcgcactga
     1921 aatcgtcgca gatgtaacca cctttgaact cacaaccgcg attgcttttc agtattttgc
     1981 caatcagcag atcgatatcg ccgttctgga agtcggtttg ggtgggcgtc tggacgcgac
     2041 aaacatcgtt gaccccctgg tttcggtcat tacccccata tcgttcgacc acatgaacgt
     2101 tctcggaaac acgcttacag aaatcgccca ggaaaaagct ggcatcatca aacctaatcg
     2161 ccctgtggtc atcgcgccac agcatgaaga agcggaaaaa gaaatcctgc gggtcgctgc
     2221 aagccaaaac gcgcctgtca cgctggcaga taaagcctac acctggcaaa tcctgagtca
     2281 cgacatcaac catcaactta tcgccattca acaaacggat acaaatcaac aaaaccccga
     2341 acagctaacg cctgtcgagt tggatatccc cttactgggc aggcaccagg ccgacaacgc
     2401 cgcgacatcc cttgccacgc tcgaaatact gcgtcaatcc ggctttaaca tcacccagga
     2461 tgatttaaaa cacggttttc aagacgtgta ctggcaaacg cgctttgaag ttctccgcaa
     2521 ggaaccgcct ttcatcgctg acgccgccca caatggcgat tcgatgcaaa agctgcgtca
     2581 ggcactggat gactacttcc ccggtttacc ctttatcctt ttatttggcg cgtcggcaga
     2641 taaaagcctg accaccatgc tggatatcat cctgccccgc gtagaagccg tggtttgcac
     2701 acaatctatc cacccccgcg ctgccgatgc ctttgaatta caaaagctga ttgagcctta
     2761 cggaataccc gtttatgctg aagtgcccgc tgaagcagga ctcgccaaag cgcttgaact
     2821 ggcgggcgac tccaaaggca tcgtcgctac cggcagcctg ttcctcgccg ccgcggcacg
     2881 tgccatttgg caccaagact atcaataaca gagagatatt atggattcaa acacccctaa
     2941 attccctttt caagccactg atcgcatcga cttttcgaac ctggtgaatc tccacgacgt
     3001 gcctgaacgt cgtccgggca aagatggcac tttcaccgcg ccagcgataa ttatcaccaa
     3061 aagccccatc ttgcccgact ccttaagtac catgaccgtc gccactgacg cgctcattct
     3121 ctccgccatc aaccaagcct gggaagaaaa caccaccatt gtcggtgttt ttgacgatgg
     3181 taaaaaagaa cagagcttct tatcactggg tgtcgaattt gcgcctggag aacccattca
     3241 aaccgacaaa gacaccgtga cttttatcgt gcaaggacga cgccgggttg aagttttgga
     3301 gattttcgtt gacgaaaatg atatgctttt cgtcaaagcg gctttgataa aaatccccaa
     3361 acgtcgttca aaagaagttc aagccttgat gcgtcaggtt ataaaatatt tcgagacttt
     3421 cgcccagtta agccaacatt tgccaccaac ggtcattcaa tttgcccgtc agcttgaaga
     3481 ccccggtgaa ttagcagaca tcgttaccgc gtccatcaac ttgaaacccg cgcagatgat
     3541 ggaaatcatc accgcggttg accccgcgga acgcattgaa accgctttac gccttatcaa
     3601 acacgaagtg tcactccttg aacttgaagc ggaaattaac gaccgcgttc aacaagaact
     3661 agaccagggc cagcgcgaaa tctatctgcg cgagaaaatc gaaaaactcc agcaggaact
     3721 aaacgatggt aaatcgacag accccgacat tcgctcgctt gaagacctgc tggaaagcaa
     3781 agagtacccc gaagaagtcg tcaaaacagc caaaaaagaa cttgaacgcc tcaaaatgac
     3841 cccgtcactc tccccggaaa gtggcatgat ttccacttat ttgcactggc ttttggaact
     3901 gccctggaac gaagaaaccc aagaccgact cgacattaag gaagcccagg ccatactcga
     3961 caaacaccat tacggcttgc ccaaagccaa agaccgcatt ttggaatatc tcgcagtgcg
     4021 ttccctgaac ccacccaaaa accgccagcc tattttgtgc ttcgttggcc cacccggaac
     4081 gggaaaaacc tccctgggta agtccatcgc ggaagcaatg ggcagaaaat tcgtccgccg
     4141 gtctttaggt ggcgtgcgcg atgagtccga aattcgcggt catcgccgga cttatatcgg
     4201 ttccatgccc ggtcgcattc tggaaaccat cagcaaggtg ggcgtggcta atcctctatt
     4261 catgcttgat gaaattgaca agctaagttc cgatatccac ggcgacccgg catccgcgtt
     4321 gcttgaagtg cttgaccccg aacaaaacta cagcttctcg gaccattacc ttgaagtgcc
     4381 ctttgacctt tcaaaagtgt tcttcatcac taccgccaac acggtgcaaa gcatcccccc
     4441 agcccttttg gaccgcatgg aaatcatcga attcccaggc tatattgaag aagaaaaact
     4501 cgaaattgcc cgacagttcc ttatcccgga acaactcgaa gaaaacggcc tgcaagacaa
     4561 atccatcgag tttaccgaag acgccctgcg cttaattatc aaaagctaca cctacgaagc
     4621 cggtgtgcgc aacctggaac gcatgatcgg cgcggtgcta cgcaaaaccg cccgcaagct
     4681 tgccgaaggc gaagctatcg aaaaaatcat caccccagaa ctggttgaag aacggcttgg
     4741 accggtggaa ttcttcccca tcaccgcgga actgcaggat gaagttggca tggcaaccgc
     4801 ggttgcctgg acggaaaacg gtggtgaaat catgcagatt gaggtactgg tcatgcccgg
     4861 aaaaggcaac ctgcaaatca ccggtcaggt aggcgagatt atgcaagagt ccgcccaggc
     4921 cgccctgagt tacatcaagt caaaagctga aaacttccaa attcccgaca gtctctttga
     4981 agaaaccgat gtacacattc atgtccccga aggctcaatc cccaaagatg gccccagcgc
     5041 gggcatcact atggcaatcg ccatgacatc cgccgtgtta ggggtgccca cgcgccacga
     5101 agtggccatg actggcgaaa tcaccctacg cggacgcatc ctgccggtag gtggtgtgcg
     5161 cgaaaaagtc ctggcagccc accgcgcggg aattaccacc attctcctgc ccacgaaaaa
     5221 ccaaaaagac ctgattgaag tgcccgcgcc tgtactagaa caactaagaa ttgtgttcgt
     5281 tgaacacatg gacgaagtct tgaaagaagc cctggctggt gaggcgattt atgccaaaaa
     5341 accaaaagaa accccgcccc gccacccccg tccgacgaaa aacaaaatgg cacaaagaag
     5401 ccaacaaaac agcgacaccc aagccgtggc ttaactaaag tcataaaaca agtcgtcaaa
     5461 aaagcacagc gaccatgctg tgcttttttt atttcaagat aatacagacg tgccctaaaa
     5521 tgtcacgtca aaatagtatt ttgataatat tatttatcgt ccggcgagag ggcggcaatg
     5581 ccgttgcgca cctgtgccag cact
//
LOCUS       DAOSFC010000066         5603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_12801_length_5603_cov_4.372386, whole genome shotgun sequence.
ACCESSION   DAOSFC010000066 DAOSFC010000000
VERSION     DAOSFC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12801_length_5603_cov_4.372386"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..761
                     /gene="msrA"
                     /locus_tag="PK791_02715"
     CDS             288..761
                     /gene="msrA"
                     /locus_tag="PK791_02715"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOH92257.1"
                     /translation="MLEQALLAGGCFWGLQAQLKAVPGVSDTLAVYTGGTTENPSYAQ
                     VCRQETGHVEAVQLKFDTEQISFEKLLEIFFQSHDPVLAERFGEQYRTTVFYANEEQN
                     KIAQKVIKAVKSSGIYPKDIDVALRPVGNVWPAEDYHQDYYAKHNVISDDDCYCR"
     gene            791..1552
                     /locus_tag="PK791_02720"
     CDS             791..1552
                     /locus_tag="PK791_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOH92258.1"
                     /translation="MTDTTLPNGNIEKRIAILIDGDNAQASLMEQIVVEASRFGNTTI
                     RRIYGDWTTTHMKSWKETLNSYAFQPFQQFSYTTGKNSTDSAMIIDAMDILHTDLVDG
                     FCLVSSDSDYTRLATRIRESGKLVIGIGKQSTPRAFVSACEVFIYTENLEERARQKKK
                     AAARQKIAAKLSTQEIELIENINRAIEMVGTDDDNWASLSEVGTSLRRIDPGFDPRSY
                     GAKQLASLVRQHKTFFDLKKVPGKSVMIIRSKEEL"
     gene            complement(1598..3157)
                     /locus_tag="PK791_02725"
     CDS             complement(1598..3157)
                     /locus_tag="PK791_02725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOH92259.1"
                     /translation="MEPQLLAIDLGTQSCRALVFDPKGNLIAKSQIPIDTFSTPEPGW
                     VEQDPELFWQIVCQACQAVWEMPSVRKDTLLGVSITTQRSTIINLGADGKPLRPAIHW
                     SDQRRTPGLKPIGGFWGLAFAITGMTPTVSYLMAEAEINWIRRNQPEIWEKTHKYMLL
                     SGYMHYRLTGELVESTGNMATYMPFDYKKQSWSGKHDWKWKAVPIEKEKLMPLVPPTT
                     LIGQITKEAAQATGIPEGLPLYAAASDKACEVIGAGCVEPNVACLSFGTTATINTTHY
                     KYVEAVPLLPPYPAAIPNAYSLEIQLYRGFWLVSWFKREFGHNEQRLAQKRGVPTEAL
                     FDDLINSVPAGSLGLMTQPYWAPGLKLPGPEAKGAVIGFGDIHTRAHVYRSMIEGIGY
                     ALREGAERTSKRSRIPIESIRIAGGGSQSPAAMQIAADIFGLPASRPHVYEASGLGAA
                     MDLAVGLDLYPDFKTAVSAMTRIHDTFEPNPKNQAIYDELYNRVYLEMYRRLKPLYHE
                     ISDITGYPPSD"
     gene            complement(3271..3828)
                     /locus_tag="PK791_02730"
     CDS             complement(3271..3828)
                     /locus_tag="PK791_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOH92260.1"
                     /translation="MKKKPSQNKSKANLKKVVAKSSKDKSKHSITRKNKRKKLSPWLF
                     FLLLIPIALAFFLINSHINKNAPDVPNEITAIQNSDALPTELSVSEAHALYTDGAFIL
                     DVREADEWHQGHIPGATLIPLENLIYAYQSLPVSEPIIVVCRSGNRSALARDFLIKVG
                     FPQVANMAGGMNSWIAAGYDLTTGD"
     gene            complement(3915..5366)
                     /locus_tag="PK791_02735"
     CDS             complement(3915..5366)
                     /locus_tag="PK791_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOH92261.1"
                     /translation="MNFGIIRYLLGRILMILSGLMVPSVVVGIIYREPFQVLKGFLIT
                     MALCLALGFLLSRNKPNKDDFYAREGLILVVLAWLMISLIGSLPYIFSGKVSSFIDAL
                     FESTSGFTTTGATVLAIEENVLPHSLLFWRSFSLVIGGMGFLFFIISLQPAIGPRGIF
                     IMRAELPGPSFGKLESRISKSIHILYAIYIGLIFAVIILLRIGKVDWFEAILLAFGTA
                     STGGFDIVPQSIAAYDSQFVHMVIAVAMMIFGMSFNLIYLLVIGKFKQFFKSEELRWY
                     LGIIISAVLLISASVFPLYTNIKTMFQDVFFTTTSIITSTAYTTVDIQKWPIFAHIVL
                     LFLMFCGGMSGSTASGLKVARIMMFGKSIKQELRRMISPERAVPIVIEGKPVDAINQR
                     STTFYIITYLAVFLVLLALISIDTQDFTSSFNAVIATLNNVGHGSDLLGPSLPYSQLS
                     VPTKLILCLAMIMGRLEIYPVLLLFFPATWRKT"
     gene            complement(5370..>5603)
                     /locus_tag="PK791_02740"
     CDS             complement(5370..>5603)
                     /locus_tag="PK791_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOH92262.1"
                     /translation="LNSENTEALEFIAHKNAKICRATIAELKFKPNILVAHIMRGIRR
                     IIPSGADQIREGDRVLVVTLGHRIAELDDILED"
BASE COUNT         1540 a         1216 c         1376 g         1471 t
ORIGIN      
        1 gcttggttgt tggttcgaat ccatccgggc ccatgtttta tttaagttcc aaaacaatgt
       61 gagattacaa attgtcagag gaccatgcgt atacgtaaaa atgtatgtaa tatttcgttt
      121 acatgctgaa tgtgaatatt ctcattgtgt aaaatgacat aaagcacttt tttgtccaaa
      181 tctataagaa aaggaaggct tgtctataga ttttgcgtga aataatgtgc tcttcgtgat
      241 atagttgcat caatgttaaa aatactttcc aaaatggaga ttgccctatg ttagaacaag
      301 ccttactcgc aggtggttgt ttttggggtt tacaagcaca attgaaagct gttccgggcg
      361 tgtcggatac gctggctgtc tacactggcg gtactaccga gaatcccagt tatgcccagg
      421 tttgtcggca agaaacgggg catgttgaag ctgttcaact gaaatttgac actgagcaga
      481 ttagttttga aaagctttta gagattttct ttcaatctca tgatccagtt cttgctgaaa
      541 gatttggtga acaatatcgc accacggttt tttatgcgaa cgaagaacaa aacaaaattg
      601 cacaaaaagt aatcaaggct gtaaaatctt ccggaatcta tccaaaagac atagatgttg
      661 ctttgcgacc tgttggcaac gtttggcccg cggaagatta tcatcaggat tattatgcca
      721 agcataatgt aatctctgat gatgactgct attgtcgtta gcctttgcgg tttcacgtta
      781 taatggggtc atgacagata caacgctacc taacggaaat attgaaaaac gcatcgcgat
      841 actcatcgat ggcgataacg ctcaagcatc tttgatggag caaatcgttg ttgaagccag
      901 ccgatttggc aatacgacca ttcggcgtat ttatggcgac tggacgacaa cgcacatgaa
      961 atcctggaaa gagacgctta attcttatgc ttttcagcct tttcagcaat ttagttacac
     1021 cacagggaaa aattctaccg actcagcgat gattattgac gctatggaca ttctccatac
     1081 ggatttggtg gatggctttt gtctcgtgtc tagtgacagc gattacacgc gcctggcaac
     1141 gcgcattcgc gaaagtggca aactggtgat tggcatcggc aagcagagca caccccgtgc
     1201 ctttgtttca gcttgcgaag tgtttattta taccgaaaac cttgaagaac gcgcccgcca
     1261 gaagaaaaaa gccgccgcgc gtcaaaagat tgctgccaag ctaagcacac aggaaattga
     1321 acttattgaa aatatcaacc gcgctattga gatggttggt accgatgatg ataactgggc
     1381 tagcctttcg gaagtgggta cctcattgcg caggattgac cccggttttg acccccgcag
     1441 ctatggtgcc aagcaactgg ccagcctggt gagacagcat aagactttct ttgaccttaa
     1501 aaaggtgccg ggtaaatcgg tgatgattat cagatcaaaa gaagagctct aatactgagc
     1561 tctttttctt ttatatgtag tttttcaaaa cgaatattta atctgaaggt ggatagcccg
     1621 taatatcgct tatttcatgg taaagtggtt tcagccggcg atacatttcc aggtaaacac
     1681 ggttgtagag ttcgtcgtaa attgcctgat tctttgggtt tggctcaaag gtatcatgta
     1741 tccgagtcat cgcactaact gcggttttga aatccgggta aagatccagt ccaactgcca
     1801 ggtccattgc cgcgcccaga ccgctggctt cgtaaacgtg tgggcgactg gcgggcagac
     1861 caaaaatatc ggcggcgatt tgcatcgcgg cagggctttg cgaaccacca ccagcaatgc
     1921 ggatggattc aatcgggatg cgactgcgct tactggtgcg ttccgcgcct tcgcgcagag
     1981 cgtatccgat gccttcaatc attgagcgat aaacatgcgc gcgcgtgtga atatcgccaa
     2041 aaccgataac cgcgcctttg gcttcgggac caggtagttt taagccaggc gcccagtaag
     2101 gctgggtcat cagaccaagc gaaccggctg gcacagaatt gattaggtca tcaaagagag
     2161 cttccgttgg tacaccccgt ttttgtgcaa ggcgctgttc gttgtgccca aactcacgtt
     2221 tgaaccacga aaccagccaa aagccacggt agagctgaat ttcaagcgag taagcattgg
     2281 ggattgcggc cgggtagggc ggaagcagag gcacagcctc gacatatttg taatgcgtgg
     2341 tgttgatggt tgctgtggta ccaaaactca agcaagcgac atttggctca acacagcccg
     2401 cgccgattac ttcgcaggct ttatcggatg ccgcggcgta caaaggcaaa ccttctggaa
     2461 tgccggttgc ctgggcggct tcttttgtaa tttgcccgat caaagtggtg ggtggtacaa
     2521 gcggcattag cttttctttt tcaatgggta cggctttcca cttccagtcg tgcttacccg
     2581 accagctttg ttttttgtaa tcaaacggca tataggtggc catattgccg gttgactcga
     2641 ccagttcgcc cgtcagccga tagtgcatat agcctgagag caacatgtat ttgtgcgttt
     2701 tctcccagat ttcgggttgg ttacgccgaa tccagttgat ttcggcttcc gccatcaagt
     2761 aggaaacggt cggagtcatg ccggttatcg cgaaggcaag tccccaaaag ccacctatcg
     2821 gctttagtcc aggggtgcga cgttggtctg accagtgaat ggcaggacgc aatggtttgc
     2881 catccgcgcc aaggttgatt atcgtggaac gctgggtggt gattgaaaca cccaaaaggg
     2941 tgtctttgcg cacgctgggc atctcccaca ccgcctggca tgcctgacag acaatttgcc
     3001 agaataattc cgggtcttgc tctacccaac ccggttcggg ggttgagaag gtgtcaattg
     3061 ggatttgcga tttggcaatg agattgcctt ttggatcaaa aacaagcgcg cgacacgact
     3121 gcgtgcccag gtcaattgcc aaaagttggg gttccatgat atctactcct taataaatta
     3181 atgatacccc taaaatactg ttttaggcgg attttgatgg ggagttgcgg gaactaagcg
     3241 gaaatcaggg aaaaaagttt tataaagctg ttagtcgcct gttgttagat catacccagc
     3301 agctatccaa ctattcatac cacccgccat gttggcaact tgcggaaagc caacttttat
     3361 cagaaaatca cgggccaggg cactgcggtt gcctgagcga cagacgacaa tgattggctc
     3421 ggaaacgggt aatgactgat aagcatatat caagttttcc agggggatta aagtggctcc
     3481 tgggatgtgt ccttggtgcc attcgtccgc ttcgcgaacg tcaagaatga aagcgccgtc
     3541 ggtgtaaagc gcgtgggctt cgctgacgct gagttcagtg ggcaatgcat ctgagttttg
     3601 tatggcagtt atttcgtttg gtacgtcggg ggcattttta ttgatgtgag aattgatgag
     3661 aaaaaatgct aaagctatgg gaatgagcag taagaaaaag agccacggac taagcttttt
     3721 gcgcttgttc ttcctggtaa ttgagtgctt gcttttatct ttagaagatt tggcaacgac
     3781 ttttttaagg tttgctttgc ttttgttttg gcttggtttc tttttcatgc tttcgtatcc
     3841 tatatgtaag agaattttac acgaaatgaa ataataaaga ttgcagaact ttaattatgt
     3901 ttttttatga ctgtttaggt ttttcgccag gtggcaggga agaaaagtaa aagcaccggg
     3961 taaatttcca gcctgcccat aatcatcgcc aaacataaaa ttaattttgt gggaacgctt
     4021 agctgactat atggcagcga tggtccgagc aaatcggatc catggccaac attattgagc
     4081 gtggcaatga ccgcgttgaa cgaactggtg aagtcctggg tgtcgatgct tatcaaggcc
     4141 agcaaaacca gaaaaacagc cagataggtg atgatataaa aggtcgtgct gcgttggttg
     4201 atggcatcaa caggtttacc ttcaatgacg atgggtaccg cgcgttcggg ggatatcatg
     4261 cgtctgagtt cctgtttgat ggatttgcca aacatcatga tacgggcgac tttcaagcct
     4321 gatgctgtcg accccgacat gccaccacag aacattaaaa acaacaggac gatgtgagcg
     4381 aagatgggcc atttttgaat gtcaacggtc gtgtatgctg ttgaggtgat aattgacgtt
     4441 gtggtaaaga aaacatcctg aaacatcgtt ttgatgttcg tatagagtgg gaaaacactc
     4501 gcggaaatca gcagtacagc actgataata atgcccaaat accagcgtaa ttcttcagat
     4561 ttaaaaaatt gcttgaattt gccaatgacc aaaagataga tgagattgaa actcataccg
     4621 aaaatcatca tggcaaccgc gatgaccata tgcacgaatt gactatcata ggcggcaatc
     4681 gactggggca caatgtcaaa gcctccagtg cttgcggtac caaacgccaa caggattgcc
     4741 tcgaaccagt caacctttcc aatgcgcaag agaatgataa cggcaaaaat taagccaatg
     4801 taaatagcat acagaatatg aatcgacttg ctaatgcgcg attccagttt accaaagctt
     4861 gggccaggca gctcagcgcg cataataaaa atgccccgtg ggcctatagc gggctgcaag
     4921 ctgatgataa aaaacaaaaa gcccatacct ccaatcacca gcgaaaaact gcgccaaaac
     4981 agcaaggaat ggggcaatac gttttcttca atggctaaaa cagtggctcc cgtggtggta
     5041 aaaccactgg tggattcgaa caacgcgtca ataaaagagc ttactttgcc actaaaaata
     5101 tacggcaatg aaccgataag cgatatcatc agccaggcca atacgaccag aattaaacct
     5161 tcgcgcgcat aaaaatcatc tttgttcggt ttattgcggc taagcaagaa tcccaaggcg
     5221 agacacagcg ccatggtaat caaaaaacct tttagcacct ggaagggttc gcggtaaatt
     5281 atgcccacca cgacggatgg caccattagg ccactcaaaa tcataagtat tctgcctaaa
     5341 aggtaacgga tgatgccgaa gttcatcgtt tagtcttcca aaatatcgtc aagttccgcg
     5401 atgcgatgcc ccagcgtaac cactaaaaca cggtcgcctt cccgaatttg atcagcacca
     5461 ctagggataa tgcgacggat accgcgcatg atatgtgcga caagaatgtt tggcttgaat
     5521 ttaagttcgg cgattgtcgc acgacaaatt ttggcgtttt tgtgggcgat aaattcaagt
     5581 gcttcagtgt tctcggagtt caa
//
LOCUS       DAOSFC010000067         5575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_12897_length_5575_cov_4.685145, whole genome shotgun sequence.
ACCESSION   DAOSFC010000067 DAOSFC010000000
VERSION     DAOSFC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12897_length_5575_cov_4.685145"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PK791_02745"
     CDS             complement(<1..850)
                     /locus_tag="PK791_02745"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOH92263.1"
                     /translation="MENLKLTSREENYSEWYNQLVLRAELADYAPVRGCMVVRPYGWA
                     LWENIQAALDKRFKETGHVNAAFPMLIPESYMTKEAEHVEGFAPELAVVTHAGGSKLE
                     EPYVLRPTSETIIGTMYSKWIQSYRDLPVLMNQWANVIRWEMRTRLFLRTTEFYWQEG
                     HTAHATYEEAEEETLRMLGVYTEFCHNEAAMPVLPGRKSDNEKFAGALATYSIEAMMG
                     DTKALQAGTSHFLGQNFAKAFDIKFLDQNNELQYCWTTSWGVTTRLIGAMVMIHGDDQ
                     GLVLPPR"
     gene            complement(854..1399)
                     /locus_tag="PK791_02750"
     CDS             complement(854..1399)
                     /locus_tag="PK791_02750"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOH92264.1"
                     /translation="MIVAVFGSASPKAGDKLYQQAFDLGQALGQAGYQVMTGGYCGTM
                     EATCKGARQTATTHTIGVTCDEIEAYRPGSANAYVLEEVRTQTLSERIDYMVRHADAF
                     IALQGGVGTLAEIMLAYNMMIIGAIERKAFVLIGESWATLGAHFFDELEGHVPAFAKE
                     LLIFVNTVDEAVLALESRNKK"
     gene            complement(1396..1716)
                     /locus_tag="PK791_02755"
     CDS             complement(1396..1716)
                     /locus_tag="PK791_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HOH92265.1"
                     /translation="MNIEDLTAKMNALVAHHGWYEPDSPKKQLPRNLAISLCLEAGEV
                     LEHYQWGDESADKEALAGELADVALYLLQLAHVEGIDLEQAILDTIARNWNRQWSDEK
                     DVEK"
     gene            complement(1783..2778)
                     /gene="gap"
                     /locus_tag="PK791_02760"
     CDS             complement(1783..2778)
                     /gene="gap"
                     /locus_tag="PK791_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOH92266.1"
                     /translation="MKTKIGISGFGRIGRLVARVAVMDPEVELVAINSTADPENTARL
                     FKYDSTHGTFDGEVSFDEGHLIVNGKAIPLFCDRDPNKLDWGKYGTEIVIDSTGAFRT
                     IDTASAHLRDTVKKVIITAPSSDAQMIVMGVNQDAYDPSQPVVSNASCTTNCLAPVTK
                     VIHENFTIEAAAMTTVHAFTNDQNNLDNRHKDPRRARGCTQSIVPTSTGAAKAMGKVI
                     PALNGKMNGFSVRIPTPNVSLVDVVFQLKESPSVEEVNALLKQAAEGELKGILGYSDE
                     PLVSIDYNGDSRSSIVDGLSTMKVGEHLIKVVAWYDNEWGYSVRCVDLAKFIHNK"
     gene            2912..4090
                     /locus_tag="PK791_02765"
     CDS             2912..4090
                     /locus_tag="PK791_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOH92267.1"
                     /translation="MATIWTIVQFVFVFGLIIFVHELGHYLMAVILGIPVAEFGFGYP
                     PKLIRLFRWRGTDFTLNAIPFGGFVRFEEEVPIDETEELSAKAIVVETPCGEPDVIIE
                     PETQEAKTKTVDSGIIGGITQAPKWKRFLILVAGPTMNFILGIIVLVIMFNAIGSPDP
                     TRVIIADIAPNSPAAQAGLEVKDLVKTINGVKIDSNEALSNQVQANLGKEIEIVVLRD
                     SQEFTTHLIPRENPPEGEGAIGIIMDNPKLPAKFGDTLKSAFGTFWNQVKTTLSLPIQ
                     LIQGAVQPEEARIVGIKGIYDMFAYAEEADQIIDNDLPIMRLSLLSIVSIAVGITNLL
                     PIPALDGGQILFLLIETITRKKMPERVANAINTVFFFLLIALMIFITFRDFINPIQLH
                     "
     gene            4100..5227
                     /locus_tag="PK791_02770"
     CDS             4100..5227
                     /locus_tag="PK791_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOH92268.1"
                     /translation="MATIMPIDKLISKLREDKIPFSNKFLHHFSDLDSPSLNKLREVW
                     FSFPVERRRALIIGMAELAEDDPLQLFESAGKLGLDDTDDDVVVAAIDLLFEADDKRL
                     IKRYLTFLRDNSRKENLRAAAANALVPYFYKCALNELDANIQAEIENALIFAYEHDRS
                     DLVRRRVLETLGYSEKPVLKEMILRALAMEQVYWTESALLAIGRTMNHEWDSTVLEYF
                     SDENPIILAQAVHAAGLLGIYSARRPLFRLLNLETDYDVRQEVIWALSQIGGDEVIER
                     LEHFANITDDEDEIELIEDSIEYLELFGTDDPELATLLNDSHFGSDDDNDFDSDDDDD
                     FFKIPMDMEEWERYVNDDDFDDEEDEFSAEFDDDEDDEDIY"
BASE COUNT         1445 a         1452 c         1223 g         1455 t
ORIGIN      
        1 aacggggtgg caaaaccaaa ccctggtcat caccgtgaat catcaccatg gcaccgatca
       61 ggcgtgtggt cacaccccaa gaagtcgtcc agcaatattg caattcgttg ttttggtcaa
      121 ggaatttgat atcaaaagct ttggcaaagt tctgccccag gaaatgcgag gtacccgcct
      181 gcaaagcctt ggtatcgccc atcatcgctt caatggaata agtcgctaaa gcgccggcaa
      241 atttttcatt atccgatttg cgaccaggca aaaccggcat tgccgcttcg ttgtggcaga
      301 attcagtata aacgccaagc atcctaagcg tttcttcttc agcttcctca taggttgcgt
      361 gcgcggtatg accttcctgc cagtaaaact cggtcgtacg caaaaagaga cgggtgcgca
      421 tttcccagcg aataacattc gcccactggt tcatcaaaac tggcaagtca cgataggatt
      481 gaatccattt tgagtacatt gtgccaatga tggtttcaga cgtcgggcgc aaaacataag
      541 gttcctcaag tttagaccca cccgcgtggg taaccaccgc caactctggc gcgaaacctt
      601 caacgtgctc agcttctttg gtcatgtacg attctggtat cagcatcggg aaagcggcat
      661 ttacgtgacc cgtttcctta aagcgtttgt ccagcgccgc ctggatattc tcccataatg
      721 cccagccata aggacgcaca accatacagc cacgcactgg cgcgtaatct gccagctcgg
      781 cgcgcaacac cagttggtta taccattctg aataattctc ttctctgctt gtcagtttta
      841 aattttccat cttttacttt ttattcctgc tttctaatgc caacactgcc tcatcaacag
      901 tgttcacaaa tattaataat tccttcgcaa atgccggcac atgcccttcc agctcatcaa
      961 aaaaatgagc accgagtgta gcccaggact caccaatcaa aacaaaagct ttgcgttcaa
     1021 tggcaccgat aatcatcata ttatacgcca gcataatttc cgccagcgtg ccaacaccac
     1081 cctgtaaagc aataaaagca tccgcgtggc gaaccatata atcaatgcgc tcgctcaaag
     1141 tttgggtgcg cacttcttca aggacataag cgttcgcgct acccggtcga taagcctcaa
     1201 tttcatcgca ggtcacgcca atcgtatgcg ttgttgcggt ctgtcgcgcg cctttgcatg
     1261 tcgcctccat ggtgccacaa tagccacccg tcatcacctg ataacccgcc tgacccaaag
     1321 cttgccccaa atcaaaggct tgttggtaaa gcttatctcc tgctttaggc gatgcactgc
     1381 caaatacggc tacaatcatt tctcaacgtc cttttcatct gaccattgcc ggttccagtt
     1441 gcgcgcgata gtatcaagta ttgcttgttc caggtcaatg ccttctacat gcgccagttg
     1501 caagaggtaa agtgccacat cagccagttc accagccagg gcttctttat ccgcgctttc
     1561 atcgccccac tggtaatgct ccaggacttc gccagcttcc agacaaagcg aaatcgccag
     1621 gttgcggggc aactgttttt tgggcgagtc gggctcgtac cacccatgat gcgccacaag
     1681 cgcgttcatt tttgcggtca ggtcttctat attcataacg aaagcgatta tatccgattt
     1741 tcacgaatat aaccgcttct cctcgtctgt ttatgtcgcg aactacttat tgtggatgaa
     1801 ttttgccaaa tccacgcagc gcacgctgta accccattca ttgtcgtacc aggcaacaac
     1861 tttaatgagg tgttcaccaa ctttcatggt tgaaagaccg tcaacaatac tcgaacggga
     1921 atcgccgtta tagtcaatcg agaccagggg ttcgtcgcta taacccaaaa tacccttcag
     1981 ttcgccttcg gcagcttgct tgagcaaagc gttgacttct tccacactgg ggctttcctt
     2041 cagttggaaa acgacgtcca ccagggaaac gttgggggta ggaatgcgca cagaaaaacc
     2101 gttcatcttg ccattcaaag caggaataac cttgcccatc gctttggctg cgccagttga
     2161 agttggcacg atgctctggg tacaacctcg cgcgcggcgc gggtctttgt ggcggttatc
     2221 cagattgttt tgatcattcg tgaatgcgtg cacagttgtc atcgcggcag cttcaatggt
     2281 gaagttctcg tgaatgactt tggttactgg cgccaaacag ttggttgtac agctggcatt
     2341 tgagaccaca ggctggctgg ggtcgtaggc atcctgattg acacccatta caatcatctg
     2401 cgcgtcagaa ctcggcgcgg tgatgatgac tttcttcacc gtatcgcgca agtgcgcgct
     2461 ggcggtgtcg atggtgcgga aagcacccgt tgagtcgatg acgatttcag tgccatattt
     2521 gccccaatcc agtttgttgg gatctcgatc acagaaaagt ggaattgctt taccgtttac
     2581 aatcagatgt ccttcgtcaa agctcacttc gccgtcaaat gttccgtggg tagagtcgta
     2641 tttaaaaaga cgtgctgtat tttcagggtc cgcagttgag ttgattgcca caagctccac
     2701 ttcaggatcc ataaccgcaa cgcgcgctac aagacgccca atgcgaccaa atccactaat
     2761 accaattttt gttttcatat ataaacctcc gttataatat cgcgtctatt atatatcata
     2821 ttcccccgta aagaataaga tttttgtaaa aagcttcggc aaattcggta ggcaaaagca
     2881 aaatgagtat aataatcccc aaggagacac tatggcaact atttggacca ttgttcaatt
     2941 cgtgtttgta ttcggattga ttatttttgt acacgaactt ggccattatt taatggcagt
     3001 tattttaggc attcccgtcg cggagtttgg ttttggctac ccgccgaagc tcatcagact
     3061 gttcagatgg cgtggcacgg acttcaccct gaacgccatt cccttcggtg gctttgtgcg
     3121 ctttgaagaa gaagtgccca ttgatgaaac cgaagaatta tcagctaaag cgattgtcgt
     3181 cgaaacacca tgtggcgaac ctgatgtcat catcgaaccc gaaacccagg aggcaaaaac
     3241 caaaacggtc gactcgggta tcatcggtgg catcacccag gcaccaaaat ggaaacgttt
     3301 tctcattttg gtcgctggcc ccacgatgaa cttcattttg ggcatcatcg tactggttat
     3361 catgttcaac gccatcggca gtccggaccc cacccgcgtt atcattgccg atatcgcgcc
     3421 caactcacca gccgcccagg ctggacttga agttaaagac cttgtcaaaa ccattaatgg
     3481 cgtcaagatt gacagcaatg aggcattgag caatcaagtt caagctaatc ttggcaaaga
     3541 aattgagatt gtggttctgc gtgattcgca ggaatttacc acgcatctta tcccacgcga
     3601 aaacccgccc gaaggcgaag gcgcgatcgg catcatcatg gataacccca aattgcccgc
     3661 caaatttggc gataccctca aaagcgcttt tggtactttt tggaatcaag tcaaaaccac
     3721 gctgtccttg cccattcaac tcattcaggg tgccgtccag cccgaagaag cgcgcatcgt
     3781 cggtatcaaa ggtatttatg acatgttcgc ctatgccgaa gaagccgacc aaatcattga
     3841 caacgacctg cctatcatgc gcctttcact gctttccatt gtcagcatcg ccgtaggcat
     3901 caccaacctg ctgcccatcc ccgcactgga tggcgggcaa atactcttcc tgctcattga
     3961 aaccatcaca cgcaagaaga tgccggaacg tgttgccaac gccatcaaca cggttttctt
     4021 ctttctttta atcgctttga tgattttcat caccttcagg gactttatca atcctatcca
     4081 gctgcactaa ggagagatta tggcaacgat tatgcccatc gataaactga ttagcaaatt
     4141 acgcgaagac aaaatcccat tttcgaacaa atttctgcat cacttttcgg atcttgattc
     4201 acccagtttg aataaattgc gcgaagtctg gttttctttt cccgttgaac gtcgtcgggc
     4261 attaattatc ggcatggcgg aactcgccga agatgaccct ttacagcttt ttgaatccgc
     4321 tggcaaactg gggcttgacg ataccgatga cgacgtggtc gtggcagcca ttgacctgct
     4381 ctttgaagct gatgacaaac gtttgattaa acgctattta actttcctga gagataattc
     4441 gcgcaaagaa aacctgcgcg cggctgctgc caacgccctg gtgccttact tttacaaatg
     4501 cgcgctcaac gaacttgatg ccaacattca ggccgaaatc gaaaatgctt tgatatttgc
     4561 ctacgaacat gaccgctccg acttggtccg tcggcgtgtt ttggaaacac tgggttacag
     4621 tgaaaaaccc gtgcttaaag agatgatttt gcgcgctttg gccatggaac aggtctactg
     4681 gaccgaaagc gccctgcttg ccatcggtcg caccatgaac cacgaatggg attcaaccgt
     4741 gctggaatat ttcagtgacg aaaaccccat tattctcgcc caggccgtgc atgccgcggg
     4801 tttgctgggc atctacagcg cccgccgtcc tttgttcagg ctgctcaacc tcgaaaccga
     4861 ttatgacgtg cgccaggaag tcatttgggc gctatcgcaa atcggtggcg atgaagtcat
     4921 cgaacgcctt gaacatttcg ccaacattac tgatgacgaa gacgaaatcg aactcattga
     4981 agattcgatt gaatatcttg aacttttcgg caccgacgac cccgaactag ccactttgct
     5041 taacgactcg cactttgggt ctgacgacga caatgacttt gacagcgatg acgatgatga
     5101 cttcttcaaa atccccatgg atatggaaga atgggaacgc tacgtaaacg atgatgattt
     5161 tgacgatgaa gaagatgaat tttccgctga atttgacgat gacgaggatg acgaagatat
     5221 ttattaatat ctaactattt agaaaacaaa cagcctgact aatttgacta tcaggctgtt
     5281 tttgtttatt tgccaggaaa aaactcaatt ctatcctggt atacaaaaaa cactgctcat
     5341 cccagcatcc cgtcacaggt aaaaaagaat gttcgcggag gcgtcttcgc cgacgctatt
     5401 aaacactttg gttaaattct attctgctca gcgagacgcc ggcaggagaa tctaaaaaca
     5461 attacaaaat cgatattaac caaacaaggt tttatttatt tagcggagaa aaaaccatca
     5521 atcactgtgt cgtgccaaaa gcgtttgctt cttccactcc ggcaaatcgc ttgat
//
LOCUS       DAOSFC010000068         5516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13067_length_5516_cov_5.173411, whole genome shotgun sequence.
ACCESSION   DAOSFC010000068 DAOSFC010000000
VERSION     DAOSFC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13067_length_5516_cov_5.173411"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PK791_02775"
     CDS             <1..505
                     /locus_tag="PK791_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92269.1"
                     /translation="FKGEMMDVKIILTGIFGTDVAIDTMPADGANFVYYRTQNADVPF
                     EPWKIDSDWNLVPEQRGGLFQALTLRQFQVILEDLPNDFLLFAEHPVIYEPERWAQVR
                     IDMERISASGARLNPLFAFNEFNQLVGQTSLANILQQYFINNSAIPGDMRHNLVFPAE
                     YIFIVTP"
     gene            508..2997
                     /gene="priA"
                     /locus_tag="PK791_02780"
     CDS             508..2997
                     /gene="priA"
                     /locus_tag="PK791_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOH92270.1"
                     /translation="MLAQSPYVDVSVNIARIDKSLSYLVPSELIGRLKPGHLVLVPLG
                     NRIAQGVVLANLEKPDVEASVVKELIALLSDELVLTEQQLELAQWLSAATMAPIGACV
                     GLMLPVGISQRADVLYSLNDLALTDELKAGFSPIQKRIFNLLKTRGALRGAQITRALP
                     KLDWRGAMLGLRKKGIVKTKNVLPPPRVTSKTVRTAALSVPVARVAEIDPNSLGATDN
                     TRTRRMAVLQLLATDPLPMDFSWIYAQTGANYADLTLLADEGLIHFKETEVWRDPLID
                     YDLPDTTIPNLTDDQQTVWDAVLPQLDDKRARPFLLHGVTSSGKTEIYMRAVQEVLAK
                     GKQALVLVPEIAMTPLTVRRFMARFPNQVGIYHSKLSDGERYDTWRRAKRGDLNVIIG
                     SRSALFMPLEKLGLIVIDECDNDSYDETERLPFYHAVETAEAYAKICDARLILGSATP
                     RVTQYYKAEKGDWQLLRLPRRVPAHLEYKSALLEGVPRMPPVHIVDMRQELIAGNRKP
                     LSRALQEALARTLDAGQQAILFLNRKGSATYVFCRNCGFVVRCPRDDVPLTFHRARRA
                     LVCHQCGYTRQMPKTCPSCGSKQIRQLGMGTERLEDFVKECFPQARVMRWDAESTREK
                     GAHELILSHFSAHRADILIGTQMLAKGLDFPKVTLVGIILAELGLNFPDFRAGERVFQ
                     LLTQVAGRAGRSPLGGQVIMQSYQPENPIIQLAAQHDFDGFYRQELAYRRELNYPPFS
                     RLTRLIVQERNQTLAQSKAEELAIELEEKTAWMGLKRTTLIGPSPCYYEKMAGKFRWQ
                     IIVRGPSPNVILEDVPLREWLMIEPNPPDLL"
     gene            3033..3536
                     /locus_tag="PK791_02785"
     CDS             3033..3536
                     /locus_tag="PK791_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92271.1"
                     /translation="MDKRREILTWTELTRLIDHLLAQFNKPYTCLLPVNQAAIIPATM
                     LAEAMEIKHLFLISVQFPLDMHRQPHDPLSIELPLFSQFPSPEQLNRHDLLLVSAHWH
                     SGREVLASQARLEREGFSADCASLFFDQSETRLEGSAPLYYAARTVNDIIYPWQGSAG
                     FYPKVVN"
     gene            3541..4986
                     /locus_tag="PK791_02790"
     CDS             3541..4986
                     /locus_tag="PK791_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92272.1"
                     /translation="MEQTNSSRPLRAIDLFVVNAYYFSVNLQTLTIVPLLMPILVERF
                     VGAELKGSVLGSLRLVSLMLSLLVHAIAAQLSDRCTSKIGRRRPFMWLSLCLETIALV
                     GLGLAISYPPGKNASILIFTTLLLASILSNIGLGPVQGLIPDLVPKAQHGRYSAVKAL
                     FELPLPVIFVAFTIAKFVGSNNFWGVILVLIAVKVLTGTIILFIPEEAISHPKTPFDK
                     QAIWRLVGMTLAFTVIIIVPGLLVRYGLPWLKTIMTDVGQFKLISAVIGVVTMFVAVV
                     LGVVISTRVSLGKDTKGRKRFSWWVINRLAFLVGGANLTSFVLYYLQERFPQLTGEAA
                     AQPTAILMAVVGLSLLIAAVVSGWLTDKFGPKPLLFLSGVLAFAAILIIVLSGRLLAV
                     YAGAAVIGISAGVFYSANWALGTRIVPRDEAGQWLGISNVAGTGAGAIGAFIGGPIGD
                     IAGYSVLILVYGVLFLVSTLALFPIKPSEPV"
     gene            complement(5025..>5516)
                     /locus_tag="PK791_02795"
     CDS             complement(5025..>5516)
                     /locus_tag="PK791_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOH92273.1"
                     /translation="VPAMLIEQALPELEAQANEILLKLSDNTMSLSFSTQREYKDKKR
                     DDKRETLDIIISDGASTRDYETYSGGEAFRINFAIRLALSRVLSMRAGARLQTLVIDE
                     GFGNQDARGRQLLIEAISMIQNDFEKILVITHIEELKDQFPNRVEISKDAEGSSTLRL
                     VLG"
BASE COUNT         1219 a         1339 c         1458 g         1500 t
ORIGIN      
        1 ctttaaaggc gagatgatgg acgtgaaaat cattctgacg ggtattttcg gaaccgacgt
       61 ggcgattgat acgatgcctg ccgatggggc gaatttcgta tattaccgca cccaaaacgc
      121 ggacgtgccc tttgaaccct ggaaaattga cagcgactgg aacttagtcc cggaacaacg
      181 cggtggtttg ttccaggcac tcaccttgcg ccagtttcag gttattctgg aagatttacc
      241 caacgacttt ctcctttttg ctgagcatcc cgttatttat gaaccggaac gctgggcaca
      301 agtacgcatc gacatggaac gtattagtgc cagtggcgcg cgcttgaacc cgctttttgc
      361 cttcaacgaa ttcaatcagt tggttgggca aaccagtttg gcgaatattc tgcagcaata
      421 ctttataaac aacagtgcta tccctggcga tatgcgccat aaccttgtgt tcccggcaga
      481 atatatcttc attgttacgc cataagcatg ttagcacaaa gtccctacgt tgacgtcagt
      541 gtcaatatag cccgaatcga taaaagctta agttatttgg tgccgtcaga actcatcggg
      601 cgactcaaac ctggtcattt ggtgctggtg cctttaggca accgaatcgc ccagggcgtg
      661 gtgctggcga acctggaaaa acctgatgtg gaagcgagcg ttgttaaaga acttatcgcc
      721 ttgctttcag atgagttggt gcttactgaa caacagcttg aactcgccca gtggctttcg
      781 gcggcgacga tggcgccgat aggagcttgt gttggcttga tgctaccggt tggcatttcg
      841 caacgcgcgg acgtgcttta tagcctgaac gatttagcct tgaccgatga attgaaagct
      901 ggtttttctc ccatccaaaa acgcatcttt aatttactca agacgcgcgg tgctttgcgg
      961 ggcgcgcaga taacgcgtgc tttgcctaag cttgactggc gaggcgccat gctgggctta
     1021 cgcaaaaagg gcatcgtcaa aactaaaaat gtacttccgc caccacgggt gacgagcaaa
     1081 accgtgcgta ccgcagcact ttcagtccct gttgcgagag tagcggagat tgaccctaat
     1141 agtctgggcg cgactgataa cacgcgcacg cgtcgtatgg cggtgcttca gcttttggca
     1201 accgacccct tgccgatgga tttctcgtgg atttatgccc aaactggcgc gaattacgcc
     1261 gatttgacgc ttttggctga tgaggggctg attcatttca aagaaacgga agtgtggcgg
     1321 gatcccttga ttgattacga cttgcctgat accacgattc cgaacttaac tgacgaccag
     1381 caaaccgttt gggatgccgt tctgccccag ctggatgata aacgcgccag gccttttttg
     1441 ttgcatggcg taaccagttc tggcaagaca gaaatttata tgcgcgcggt gcaggaagtt
     1501 ttagcaaaag gcaaacaagc tctggtgctg gtacccgaaa ttgccatgac gcccttaacc
     1561 gtccgccgtt ttatggcgcg tttcccaaac caggtgggta tttatcactc gaaactctcc
     1621 gatggcgaac gctacgatac ctggcgcagg gcaaaacggg gtgacctgaa cgtgattatc
     1681 ggttcgcgca gtgccttgtt tatgcctttg gaaaaactcg gtttgattgt gattgatgag
     1741 tgcgataacg attcttatga cgaaaccgaa cgtctgcctt tttatcacgc ggtggaaacg
     1801 gctgaagctt atgccaaaat ctgtgacgcg cgattgattc tcggcagtgc gacacctcgc
     1861 gttacgcagt attacaaggc ggaaaaaggc gactggcagc ttttgcgttt gccccgtcgc
     1921 gtgccggcac atcttgagta caagtccgct ttgttggagg gggtaccccg tatgccaccg
     1981 gtgcatatcg tggatatgcg ccaggaactt atcgcgggca accgcaaacc gctttcgcga
     2041 gccttgcagg aggctttagc cagaactctg gacgcgggtc agcaagctat tctcttcttg
     2101 aatcgcaaag gcagtgcgac ttacgtcttt tgtcgcaact gtggctttgt ggtgcgctgc
     2161 ccgcgggatg atgtgccact cacgtttcat cgcgcgcgcc gggctttggt ttgtcatcag
     2221 tgtggctata cccgccagat gcccaaaact tgcccgtctt gtggcagcaa gcaaatccgt
     2281 cagctgggga tgggcacgga acgccttgaa gattttgtaa aagaatgctt cccacaggcg
     2341 cgggtcatgc gttgggatgc tgaaagcacg cgcgaaaaag gcgcgcatga actcatcctt
     2401 tcgcatttta gcgcgcatcg ggcagatatt ttaatcggca cccaaatgct ggcaaaaggg
     2461 cttgatttcc ccaaagtcac tttggtgggc atcattttgg cggaactggg tttgaacttt
     2521 ccggatttcc gcgctggtga aagggtcttt cagctgctga cccaggtagc aggccgggcg
     2581 gggcgcagtc ccctgggtgg gcaggtcatc atgcaaagtt atcaaccgga aaacccgatt
     2641 attcagctgg cagcgcaaca tgattttgac ggtttttatc gccaggaact ggcttaccga
     2701 agagaactta actatccgcc ctttagtcgt ctgacgcgtt tgattgtgca agaacgtaat
     2761 caaactttgg cgcagagcaa ggctgaagag ctggcaattg aactcgaaga aaaaacagcc
     2821 tggatgggct tgaaacgtac cacgcttatc ggtccatcgc cctgttatta cgaaaagatg
     2881 gctggcaaat tccgctggca aattattgtg cgtggaccaa gccccaacgt tattctggaa
     2941 gatgttccct tgcgtgagtg gctgatgatt gaacccaacc caccggattt gttgtaaatc
     3001 gggcttgata tgcgctaaat gaaggagttt tgatggacaa gagaagagag atactcacct
     3061 ggacggaatt aacacgctta attgaccatc tcctggcaca atttaacaag ccttatactt
     3121 gccttttgcc tgttaatcag gctgccatca ttcccgcgac gatgcttgca gaggcaatgg
     3181 agataaaaca tctctttcta atatctgttc aatttccttt ggatatgcat cgtcagcccc
     3241 acgacccgct ctccattgaa ctgccacttt tttcgcaatt tccttcacct gagcaattaa
     3301 accgacatga cttactcctg gtgagtgcgc attggcactc cgggcgcgaa gttctggcaa
     3361 gtcaggcacg cctggaacgc gaaggcttca gcgcggattg tgccagcttg tttttcgacc
     3421 agagtgaaac ccgtctggaa ggcagcgcgc ccctttatta cgccgcgcgc accgtgaacg
     3481 acatcattta tccctggcaa ggcagtgcag ggttttatcc caaggtggta aattaatcct
     3541 atggagcaaa caaacagcag ccgtcctttg cgggctattg acttgtttgt tgtcaatgcc
     3601 tattatttct cggtaaattt gcaaacactc accatcgtgc ctttgttaat gcctatttta
     3661 gtggagcgct ttgtgggcgc ggaactcaaa ggcagtgttt tgggctcctt gcgcctggtg
     3721 tcgctcatgc tcagtttgct ggtgcatgcc atcgccgcgc aactttcaga tcgctgtacc
     3781 agtaaaatag gtcgtcgccg accttttatg tggctcagtt tgtgcctgga aacgattgct
     3841 ttggtgggtt tgggcttggc aatcagctat cctcccggaa aaaatgccag cattttaatc
     3901 ttcacaaccc ttcttttggc atccatactt tcgaacattg gcttagggcc cgttcaggga
     3961 ttaattcctg atttggtacc aaaggcgcag catggcagat attcagcggt gaaggctctc
     4021 tttgagttgc ccttgccggt tatttttgtc gcctttacaa tcgcgaagtt tgtcggcagc
     4081 aacaattttt ggggtgtcat tttggttttg attgccgtca aggtgctgac ggggaccatc
     4141 atccttttca ttcccgaaga ggctatctca caccctaaaa caccttttga caagcaggct
     4201 atttggcgac tggtaggtat gacgctcgct tttacggtga ttattattgt ccccggactg
     4261 cttgtgcgct atggcttacc atggctcaaa acaataatga ctgatgtcgg gcagtttaaa
     4321 ctgattagcg cggttattgg cgtggtgacg atgtttgttg ctgtggtact gggtgtcgtg
     4381 atatcaaccc gcgtgagcct gggcaaagat accaaaggtc gcaagcggtt ttcctggtgg
     4441 gtaatcaaca gactggcatt tttggttggt ggggccaacc tgactagctt tgtactttac
     4501 tacttacaag agcggtttcc tcagttgact ggcgaagctg ccgcgcaacc cactgctatt
     4561 ttgatggctg tggttgggct gtccttatta attgcagcgg ttgtttccgg ttggcttact
     4621 gacaaatttg gccccaagcc ccttttgttt ctgagcgggg ttttagcttt tgcagctatt
     4681 ttgatcatag tactgtctgg gcgtttgctt gcggtttatg ctggcgcagc ggtgataggc
     4741 atttcagcgg gtgtttttta ctcggcgaac tgggctttgg ggacacgcat tgttccgcgt
     4801 gatgaagcgg gacaatggct ggggatttct aacgtggctg gtaccggtgc cggcgcgata
     4861 ggggcattta tcggtgggcc gataggcgat attgcgggtt acagtgtgtt gattttggtt
     4921 tatggcgttc tctttttagt ttccacgctg gcgcttttcc ccatcaaacc ttcggagccc
     4981 gtttagattg caagattgat gggtatccta ttcgggaaat ggacttatcc taaaaccagt
     5041 cgcagggtcg aagaaccttc agcgtctttg ctgatttcca cgcggttagg gaactgatct
     5101 tttagttctt caatgtgggt aatcaccagt attttctcaa aatcattttg aatcatgctg
     5161 attgcttcaa tcagcaactg ccgaccgcgg gcatcctgat tgccaaaacc ttcatcaatc
     5221 accagcgttt gcaaccgggc accggcgcgc atacttagga cgcgcgaaag cgcgagtcga
     5281 atcgcgaagt taatccgaaa ggcctcgcca cccgagtagg tctcataatc gcgcgttgac
     5341 gcgccatcgc tgatgatgat atccagcgtt tcgcgtttgt catcgcgttt tttgtctttg
     5401 tactcgcgtt gcgtgctaaa gctgagactc atggtgttat cactcaactt gagcaaaatc
     5461 tcgttggctt gcgcttcaag ttcgggcagg gcttgttcaa tgagcatggc gggcac
//
LOCUS       DAOSFC010000069         5516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13068_length_5516_cov_4.208936, whole genome shotgun sequence.
ACCESSION   DAOSFC010000069 DAOSFC010000000
VERSION     DAOSFC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13068_length_5516_cov_4.208936"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PK791_02800"
     CDS             <1..390
                     /locus_tag="PK791_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH92274.1"
                     /translation="GELASNGSVTWQFDRKAIYSVVSDGKNFDKAFEAALEAGADDVT
                     EDDGFIEIVGSIETFKAIHDSLAAAKLKVEEAGLRFVAQNEIELDLEQTLSTMKTIMA
                     LEDLDDVQNVYSNLKITDEAIAAMEND"
     gene            396..896
                     /gene="ruvC"
                     /locus_tag="PK791_02805"
     CDS             396..896
                     /gene="ruvC"
                     /locus_tag="PK791_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOH92275.1"
                     /translation="MLVLGIDPGIATTGYGFVKTNPAGNPELVAYGVLSTSATSSTSM
                     RLTSLYDQVRELIRQYQPDACALEKLFFQKNIKTAMTVSEARGVISLCISQAGFEPAE
                     YTPSEVKQAVTSNGNAPKSQVQEMVKFILEMDEIPKPDDAADALAIAICHLSHARYKR
                     LIDEQG"
     gene            883..1719
                     /locus_tag="PK791_02810"
     CDS             883..1719
                     /locus_tag="PK791_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH92276.1"
                     /translation="MNKADMITSTSNPQVKFLRSLHLAKHRSESGLFLVEGLKLVGQA
                     FDSNAEIQQIIISPEWLISDYGKSLVDLAIKKHIDILELSTNAFESIARKDKPQGIAA
                     LVKQNWRDINTLNLNDEKQKRICLAIEAIQNPGNLGTVLRTCDATGVSALFLLPNSTD
                     PYDPVAVKASMGAIFTTPIFKLDYAQLAQITRALDDLSIIGTSDKAEQNAFTYPYSDN
                     TLLLIGSEREGLSAQLSALCHEMVRIPMIGACDSLNLSMATGIILYEIFNQHNGLKRN
                     HD"
     gene            1712..2287
                     /gene="ruvA"
                     /locus_tag="PK791_02815"
     CDS             1712..2287
                     /gene="ruvA"
                     /locus_tag="PK791_02815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOH92277.1"
                     /translation="MINSISGKVSQISDNAITIDISGLGFEVFVTHATATELSLGQSI
                     FLHTHFIVREDLMALYGFESLEEKTMFIHFLGVGGVGPKSAMAILSTLSMDNIRRAII
                     SEEPDFFSKVPGIGKKTAQKILIHMQGKVPTLPGESYKRMSDVDDSVIEALINLGYSV
                     VEAQAAVQALPKDTPDTIEDKLRVTLQYFDH"
     gene            2312..3313
                     /locus_tag="PK791_02820"
     CDS             2312..3313
                     /locus_tag="PK791_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOH92278.1"
                     /translation="MALSRQLGIDLGTFNTQIAEGNKILAQEPTIVAIVVDEMKLVEY
                     GTTALGMLGRVPDSLEVVRPLRNGVIAEYELTEIFVRELMRKVTGPTLFFKPKIIITV
                     PYGITSVERRAVQEAGLGAGAREVYLIPQPLAAAIGIDLPIKTPTGNMVISIGAGTTQ
                     AAIISMNGLVSAETSPVAGLRLDDAIINTVRKKYGLIIGQPTAEQLKLRIGSALPVED
                     EHTMEVQGQDQVSGLPRPVSLSTNEIVDALEQPLQEIVNVIKRVLEKTPPELISDIID
                     RGVALCGGSSLLKGLDKYLTVALGIPAYVVENPVTCTVEGAAKAFDSLDTILRSQNI"
     gene            3390..3764
                     /locus_tag="PK791_02825"
     CDS             3390..3764
                     /locus_tag="PK791_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92279.1"
                     /translation="MKAASIISYIVAGVIIFFSVLIAWSAFAPDVPDSRLLVGVFGLL
                     IGFAFIYAGARFNHRAKKQAQEQNVNINIDLPGQVQINAMTCQACGAPINTNDISIVN
                     GAPMVHCKSCGTSYQITEEPKW"
     gene            3837..>5516
                     /locus_tag="PK791_02830"
     CDS             3837..>5516
                     /locus_tag="PK791_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92280.1"
                     /translation="MNKKNLFISLITVLMLIILPVSSVYAQSYRFKVTRYNVQAIIEA
                     DGTLTLLYEMVFENDPNGHAIDFVDLGLPSDDYSLSNISATINGNLVPEINHSSYVDG
                     AELALGKYQIPPGGTGTVFAKVVGISGVLFPYDQGDRENYVNFQFMPNYFDADADKSK
                     NTDYTLSIVLPPDVGDNEGVYYYPERWPGDKDALATLTLEDKRVMYEWHTTNANTRTE
                     YLFGVAFPASAVPESAILSFKEFPQRENDTGSNSYSLFSQIFGRICGSMTFLVPTAAV
                     ILISILASTAQKKASEKRKLKYLKPTLSIEGHGIKRGLTAVEAAILLEQPLDQVLTMI
                     LFGLLKKEAITVLQQDPLKIQATNPLPKGLHGYETQFINAMSHEKISTQRTGLQALMV
                     YLIESLEQKMKGFSAADTKAYYKDIVQRAWSAVEGANTPEIKSAQFEHTLEWTMLDKD
                     FSDRTTRTFMDTPVFVPRWWWRYDNSYRPFMSSSASDTAGTGKVASSVPSSSGSRPSF
                     SLPSIPGASFAASVINGGANMTKSIIGDSKVFTGNVTNQINPIPKVTSSGSG"
BASE COUNT         1585 a         1349 c         1178 g         1404 t
ORIGIN      
        1 ggcgaactgg cttcaaacgg ctctgttacc tggcagtttg accgcaaagc catctattct
       61 gtcgtttccg acgggaaaaa ttttgacaaa gcctttgaag ctgctctgga agctggcgcg
      121 gatgacgtca ccgaagatga tggcttcatc gaaatcgtcg gttccattga aaccttcaaa
      181 gccattcacg acagccttgc tgccgccaaa ctaaaggttg aagaagccgg attgcgcttt
      241 gtcgcacaaa atgaaattga acttgatttg gaacaaaccc tttcgaccat gaaaaccatc
      301 atggcccttg aagaccttga cgacgtacaa aacgtctact caaatctcaa aattacagac
      361 gaagccattg ccgcaatgga aaatgactag cttcaatgct tgttttaggt attgacccag
      421 gtatcgccac aaccgggtat ggctttgtaa aaacaaaccc agccggaaat cccgaactcg
      481 tagcctatgg tgtactcagc accagtgcta cgagttcgac ttctatgcgc ctgaccagtc
      541 tttatgatca agtacgtgaa ctaattaggc aatatcagcc cgatgcatgc gcacttgaga
      601 aactgttctt ccagaaaaac attaaaacag cgatgaccgt cagcgaagca cgtggcgtca
      661 tcagcctttg cattagccag gctggttttg aacctgcgga atacaccccc agtgaagtta
      721 aacaagccgt tacatccaac ggaaatgcac caaaatcgca ggtgcaagag atggttaaat
      781 ttatccttga aatggatgaa atccccaaac ccgatgatgc cgcggacgcg ctcgcgattg
      841 cgatttgcca cctttcccac gcgcgctaca agcgtctgat tgatgaacaa ggctgatatg
      901 atcacgtcta catcaaaccc acaagtaaaa tttcttcgtt ctcttcatct ggcaaaacac
      961 agaagtgaat cgggattatt tctcgttgaa ggcttgaaat tagtcggtca agcttttgat
     1021 tcaaacgctg aaatacagca aattatcatc tctcctgaat ggctgataag cgattatggg
     1081 aaatcccttg ttgacctggc aataaaaaag catattgaca ttctcgaatt aagtactaat
     1141 gcatttgaaa gtatcgcccg caaagacaaa ccccagggca tcgccgccct ggtaaagcaa
     1201 aactggcggg atataaacac gctaaatctc aacgatgaaa aacaaaaacg catctgtctt
     1261 gccatagaag ccattcaaaa tcctggaaac ctgggcaccg ttttgcgcac ttgtgatgct
     1321 accggtgtat cagctttgtt cctgctgcct aattcaaccg acccatacga ccccgtcgca
     1381 gtcaaagctt ccatgggcgc gatctttaca acgcccattt tcaagctaga ctacgcgcaa
     1441 ctcgcgcaaa taacgagagc actcgatgat ttgtcaatta ttggcacgtc cgataaagcc
     1501 gaacaaaacg cattcaccta tccatatagc gataatacgc ttttgcttat tggctctgaa
     1561 cgtgaaggcc tatcagccca actcagcgcg ctatgtcatg aaatggttcg tatccccatg
     1621 attggcgcat gtgattcgct taatttatcc atggcaacgg gtataattct ttatgagatt
     1681 tttaaccaac acaacggatt aaagagaaac catgattaac agcattagtg gcaaagtaag
     1741 tcaaatcagc gacaacgcga taactatcga tatttccggt ttaggctttg aagtctttgt
     1801 tacccatgct accgccacgg aacttagcct tggacaatcc attttcttgc atacacactt
     1861 tatcgtccgg gaagacctca tggcacttta tggctttgaa agccttgaag aaaagaccat
     1921 gttcattcac tttttaggcg ttggcggtgt gggaccaaaa tcggcaatgg ccatcctttc
     1981 caccttgtcg atggataata ttcgtcgcgc tatcatcagc gaagaacccg atttcttcag
     2041 caaagtaccc ggaataggca aaaagaccgc gcaaaaaatt cttatccaca tgcaaggtaa
     2101 agtccccacc ctgccaggtg aaagctacaa gcgaatgagt gatgtggacg attctgtgat
     2161 tgaagcactc atcaatttgg gatacagcgt tgtagaagcc caggccgcag tccaggccct
     2221 gcccaaagac acgccagaca ccattgaaga taaacttaga gtaacattac aatactttga
     2281 ccactaaacc cgagttacga ggaaaacgat tatggcatta tcaagacaat taggcataga
     2341 cttaggcacc ttcaatacac aaatcgctga aggaaacaaa atattagcgc aggaaccgac
     2401 catcgtcgcc attgttgttg acgagatgaa actggttgaa tacggcacca ccgccctggg
     2461 tatgcttgga cgcgttcctg actctcttga agttgtcaga ccattgcgga atggcgtcat
     2521 tgcagagtat gaactcaccg aaatttttgt tcgcgaactg atgcgcaaag tcaccggtcc
     2581 aacattgttt ttcaaaccta aaatcatcat cactgtacct tatggcatca ccagtgtcga
     2641 acgtcgtgcc gtccaggaag ctggccttgg tgcaggtgcg cgcgaagttt atcttattcc
     2701 ccaaccgctg gctgcagcga taggcatcga tttgcccatc aaaacaccaa ccggtaacat
     2761 ggtcatcagc atcggcgctg gtacaacaca agccgccatc atctcaatga atggtcttgt
     2821 cagtgcggaa acctcgcctg tcgctggttt aaggctcgat gacgccatca taaacacggt
     2881 acgaaaaaaa tacgggctaa ttattgggca acccactgcg gaacaactta aactgcgcat
     2941 tggttctgcc ttgcctgttg aagatgaaca caccatggaa gttcaaggcc aggaccaggt
     3001 gtcagggctc cccagaccgg tcagcttgag tacgaacgaa attgtggacg cgcttgagca
     3061 acctttgcag gaaatcgtga atgtcatcaa acgtgtcctt gaaaaaacac cacccgagtt
     3121 gatatcagat attatcgacc ggggtgtcgc gctgtgtggc gggtcatcac tgcttaaagg
     3181 tcttgacaaa tacctgaccg tcgcgctggg tattcccgcc tatgttgtcg agaatccagt
     3241 tacctgcact gttgaaggtg ctgccaaggc ttttgacagt ctcgatacca tattacgcag
     3301 ccaaaacatt taaacaagct agataatcta gcctgtgagc acggaaaatg gtttataaat
     3361 atgaataagg aaagcaaaag gagtatctct tgaaagcagc aagcattatt tcttatatcg
     3421 ttgcgggggt tattatcttt ttttcagttc taatcgcctg gagtgcattt gctccagatg
     3481 ttcctgattc ccgcttactt gtcggtgttt ttggtttgct gattggcttt gccttcattt
     3541 acgccggcgc gcgctttaat catcgtgcaa aaaagcaagc ccaggaacaa aatgtaaata
     3601 tcaatattga tttacccgga caggttcaga ttaacgccat gacttgccag gcttgtggcg
     3661 cacccattaa caccaacgac atctctatcg tcaatggcgc gccaatggtg cactgtaaaa
     3721 gttgtggcac aagctaccag atcaccgaag aacccaaatg gtaaagtttt gtccgcctta
     3781 cgcaaaatca tgttataggc tgataattac accagaagac taagtacgga gtttgcatga
     3841 ataaaaaaaa ccttttcatt agcctaatta cagtgctaat gcttatcatc ttgcctgtaa
     3901 gcagtgtcta tgctcaaagt tatcgtttta aagtaacccg ttacaacgtt caggcgatca
     3961 ttgaagccga tggcacactt acgcttcttt atgaaatggt tttcgaaaac gaccctaacg
     4021 gtcatgcgat tgattttgtc gaccttggcc tgccaagtga tgattattca ctcagcaata
     4081 tcagtgcgac aattaacgga aatctcgttc cagaaataaa ccattccagc tatgtggatg
     4141 gcgctgaact ggcgcttggt aaatatcaaa ttccacctgg tggtactggc accgttttcg
     4201 ctaaagtggt tggcatttcg ggtgttctct tcccgtatga ccagggcgac cgtgagaatt
     4261 acgtcaattt ccagttcatg cctaattatt ttgacgctga tgccgataaa tcaaaaaata
     4321 cggattacac cctttccatc gtcttacccc ccgatgtagg cgacaatgaa ggcgtatatt
     4381 attaccctga acgctggcca ggtgacaaag acgctttagc cacactgact cttgaagaca
     4441 aacgtgtcat gtacgaatgg cacaccacta atgccaacac gcgcactgaa tatctctttg
     4501 gcgttgcttt tcctgcttct gccgttcctg aatcagccat cttaagtttt aaagaatttc
     4561 cacaaaggga gaatgatact ggatcaaatt cctactcgtt attctcacaa attttcggaa
     4621 gaatctgcgg gagcatgact ttcttagttc caactgccgc tgttatactc atatccattc
     4681 ttgccagtac agcacaaaag aaggcttcag aaaaacggaa gttaaaatat ttaaagccta
     4741 ccctgtccat tgaaggccac ggcattaaac ggggtttaac agctgttgaa gctgccatcc
     4801 tgctggaaca acccctggac caggttctaa ccatgatact gttcggttta ctgaaaaaag
     4861 aagccattac agtgcttcaa caagatcccc taaaaattca agccaccaac ccactaccca
     4921 aaggcctgca tggctacgaa acacagttta ttaacgccat gtcacatgaa aaaatttcta
     4981 cacaaagaac tggcttacag gccttgatgg tgtatctaat tgaatctctt gagcaaaaga
     5041 tgaaaggttt cagcgccgct gatacaaaag cttactacaa agatatcgtt cagcgggcat
     5101 ggagcgcggt tgagggcgcg aatacacccg aaattaaaag cgcacaattc gaacatacgc
     5161 ttgaatggac catgcttgat aaagactttt cggaccgcac cacacgcacc tttatggaca
     5221 cgcccgtctt cgttccacgt tggtggtggc gctatgacaa cagttatcgt ccatttatga
     5281 gcagttctgc ttcggataca gccggcactg gcaaagttgc gtcttctgtt ccgagttcct
     5341 ctggatccag accgagcttc agcttaccca gtatccctgg ggcaagtttc gctgcttctg
     5401 ttatcaatgg tggcgcaaac atgacaaaaa gcattatcgg tgattcaaaa gtgttcactg
     5461 gtaatgtaac caatcaaata aaccccattc ccaaagtcac cagttctgga tcaggc
//
LOCUS       DAOSFC010000070         5510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13084_length_5510_cov_4.607149, whole genome shotgun sequence.
ACCESSION   DAOSFC010000070 DAOSFC010000000
VERSION     DAOSFC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13084_length_5510_cov_4.607149"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(250..1014)
                     /locus_tag="PK791_02835"
     CDS             complement(250..1014)
                     /locus_tag="PK791_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOH92281.1"
                     /translation="MKITTFNVNGIRAIVKKEGIGWIEDLDADVLGLQEIKAKPEQVP
                     AKLNKLPGYKVIWNSAERPGYSGTAVFYRDEPKSVSLGIDYPMFDEEGRVIRLEYPDF
                     FLYNIYFPNGGEENRRVPEKLQFYAKLLEETDELHKSGKFVIITGDFNTAHREIDLEH
                     PQANSKTTGFLPEERAWIDKYLEHNFVDAFRELYPDREKAYTWWSYRTAARLRNVGWR
                     LDYYLISKELMPRVQDVIIHDDIMGSDHCPVSLILK"
     gene            1052..2482
                     /locus_tag="PK791_02840"
     CDS             1052..2482
                     /locus_tag="PK791_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOH92282.1"
                     /translation="MKDYTKSTGTKAYKAFTSLLISLLIISISLGAFGVQPTQAQATT
                     YPEYVVQAGDSLLWIASKFNTTLEAIMQVNQIGPDSTLHPGDKIKIPSLAGLEGVLSS
                     EYVGLGTSINSLSRRSQVELGKLIRLNRITSPSELFIGREIFLTDVDQETPSTASAIL
                     EGQTFLELAIKSGQNQWALSHRNLLTNPNFALPQDSYYVPSANTQSDTLAVPGIGSVS
                     IDNLPLRQGETYVLHVQSPSNVEIEAELVNQSPQFTDLGEGKQIAFGGINALTEPGVY
                     PLTMTFRRDNGYVYRFDQLIVVKSGHYPEETLLVDPATMDEENIRAENAIFYEIIKPI
                     TPVQYWDGVFTSPAQDIDCITSKFGTRRTYNNDPNIYYHTGLDFGFCKGIDVYAPAAG
                     KVVGVLPDLIIRGNTIVIDHGLGIYSIYMHLEKMMVEEGTMVQPGQLIGLIGTTGRST
                     GPHLHFEINAQGVPVSPATWLKRVFP"
     gene            complement(2543..2624)
                     /locus_tag="PK791_02845"
     tRNA            complement(2543..2624)
                     /locus_tag="PK791_02845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2588..2590),aa:Leu,seq:gag)
     gene            2686..3675
                     /gene="plsX"
                     /locus_tag="PK791_02850"
     CDS             2686..3675
                     /gene="plsX"
                     /locus_tag="PK791_02850"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOH92283.1"
                     /translation="MKTTIVLDAMGSDDRPQPELIGAIQASASGDINIILVGDETILK
                     PALDELPGDKTHISIVHAPDVLAMDDDIRQARSKKQNTMAVGMQLVKDGKAQAFVTAG
                     NTGMAMYFGTKIFGLIKGVNRPCLCANFPTRNGQCTVLDIGANAECRPDFLVQFGQMG
                     AVYARLMNNISKPRVGLISNGEEEHKGNDLVKSAHPLMQKSVPGFIGNVEGKEIFGGE
                     VDVAVTDGFTGNVFLKSTEAVAKLLLLTIKDSLMSSTRTKIAGLLAKPAFKKVRSLLD
                     PNEIGAAPLLGLNALVFVGHGRSNSDAIVSAINQARKAIEADMLVELQNAIKR"
     gene            3688..4380
                     /locus_tag="PK791_02855"
     CDS             3688..4380
                     /locus_tag="PK791_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92284.1"
                     /translation="MRLIINEKKIKRNKKIAGAFTIASLAVLGTGLFFAFKSDMILWS
                     YVALIVGFVLTQISMYYSSRFSRSPRYDEVMHKALETIRGDYSFYVYTTTVPNLLLGP
                     NKFYALHPIVTPGTISYVNGKWREKGRGILMRIIGQENLGNPERELAIQIQGLQDKLN
                     ENGLVYSEQPPIVPLLVLLTDKTVIGEMEDAPIQHVKINELKRTIRRFDREDPEGVIP
                     EEKMEQIKSILG"
     gene            complement(4470..5195)
                     /locus_tag="PK791_02860"
     CDS             complement(4470..5195)
                     /locus_tag="PK791_02860"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOH92285.1"
                     /translation="MTEERLQKILAAAGYGSRRACELLIDAGKVRVNGKLAKLGDKAD
                     ADKDAIMVDGKLLRGLQKKVYIAIYKPRGYLSDYDENFPRPTVLDLIDVPEQLFPVGR
                     LDFDSEGLVLMTNDGELANRLTHPRYEHEKEYEVLVNHMPDEEQLSIWRRGVVLEDGY
                     RTRPAGVEFINKVHNGAWLRITLKEGKKRQIRLTGLRIGLPVRKIKRVRIGTLKLGNL
                     KPREWRYLTDKEVATLRKYVGLN"
     gene            complement(5195..>5510)
                     /gene="scpB"
                     /locus_tag="PK791_02865"
     CDS             complement(5195..>5510)
                     /gene="scpB"
                     /locus_tag="PK791_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOH92286.1"
                     /translation="TTRLSQAALEALAIIAYKQPTTRPEIDSIRGVNSDGVVRTLLSR
                     GLIEELGRSEAAGRPILYGVTPEFLQHFGLESLEQLPELNLDELLQGKAESEEVAEIL
                     KD"
BASE COUNT         1513 a         1431 c         1174 g         1392 t
ORIGIN      
        1 accttgaaac tctcgacaga ttcgttgaag tgatgctcaa gattgccgaa gaagcgcgaa
       61 cgcaaccaga attgctcaaa aacgcgcctc acaacgcgcc catcaaacgc gctgatgaac
      121 tcaaagcggc aaaagaactg gttctacgtt gctatcctaa gtattaagtg aaaagctaag
      181 taaattagaa gtaatgataa gaggctgtct ttattaggca gcctcttgtt gtattgaaac
      241 aaaacttgct tattttagaa tcagcgacac cggacagtga tcagagccca tgatatcgtc
      301 atggataatc acatcctgta cgcgcggcat aagctctttg ctaatcaaat aatagtccaa
      361 acgccagccc acattccgca atcgcgcggc cgtgcgataa gaccaccagg tgtaggcttt
      421 ttcgcggtca ggataaagct ccctgaaggc gtccacaaag ttatgttcca gatacttgtc
      481 aatccaggca cgttcttcgg gcaagaagcc ggttgttttt gaattcgcct gcgggtgttc
      541 aaggtcaatt tcacggtgcg cggtgttaaa atcgcccgta ataatcacaa atttcccgct
      601 tttgtgaagt tcatcggttt cttccaaaag cttggcgtaa aactgcaatt tttcgggcac
      661 acggcggttt tcttcgccgc cattcggaaa ataaatgttg taaagaaaaa agtcgggata
      721 ttccagacga ataacacgcc cttcttcatc aaacatcgga taatcaatgc ccaaactcac
      781 cgactttggt tcatcgcgat agaaaacagc agtaccacta tagcccggtc gttcggcaga
      841 attccaaatc accttatagc ccggaagctt attcagtttg gctggcacct gttcaggctt
      901 agccttaatt tcctgtaaac caagcacatc cgcatccagg tcttcaatcc atcctatgcc
      961 ttcttttttg acaatagccc taatgccatt cacattaaac gttgtaatct tcataaaaca
     1021 ccttttcaag cctatggtaa acttattttc aatgaaagat tataccaagt caacgggcac
     1081 aaaagcttac aaagctttta cttccctttt aatttccctg cttatcataa gcataagtct
     1141 gggcgctttt ggcgttcaac ccacccaggc ccaggccaca acctacccgg aatacgttgt
     1201 gcaagcgggc gattcgctct tgtggattgc ttcaaaattt aacaccaccc ttgaagccat
     1261 catgcaggtc aaccaaatcg gacctgatag cacccttcac cctggtgaca aaatcaaaat
     1321 tcccagtttg gcaggtttgg aaggcgtgct cagcagcgaa tatgtcggtt tgggcaccag
     1381 cattaacagc cttagccggc gcagtcaggt tgaactaggc aaactcatcc gcctgaaccg
     1441 tatcaccagc ccatctgaac ttttcatcgg acgcgaaatc tttttgactg acgttgacca
     1501 ggaaacccca tcgaccgcca gcgcgattct ggaagggcaa acgtttttag aacttgctat
     1561 taaatcagga caaaaccagt gggcgcttag ccacagaaat ttgctgacca accccaactt
     1621 tgccctgccc caggacagtt actacgttcc cagcgcgaac acgcaaagcg acacactcgc
     1681 tgtgcctggc atcggctcgg tttcaatcga taacctgccc cttcgccagg gagaaaccta
     1741 cgtacttcat gtgcaaagtc cgtcaaacgt tgaaattgaa gcagaactgg ttaatcaatc
     1801 gccacagttt acagatttgg gcgaaggcaa gcaaatcgct tttggtggca tcaatgcgct
     1861 caccgaaccg ggcgtttatc cccttaccat gactttccgt cgcgataatg gctatgtcta
     1921 ccgttttgac cagctcattg tcgtgaaatc aggccattat cctgaagaaa cgctccttgt
     1981 tgacccagcc accatggatg aagagaacat ccgcgctgaa aacgctattt tctatgaaat
     2041 catcaaacca atcacacctg tacaatactg ggatggcgtg tttacatcgc ccgcccagga
     2101 tattgactgt attacctcta agtttggtac ccgacgcact tacaacaatg accccaacat
     2161 ctactaccac accggtttag actttggctt ctgcaaaggc atcgacgttt atgcgccagc
     2221 cgctggcaaa gttgttggcg ttttgcctga tctcatcata cggggcaaca ccattgtcat
     2281 tgaccatgga cttggcatat atagcattta catgcatctg gaaaaaatga tggtcgaaga
     2341 aggcacgatg gtccagcctg gtcagctcat tggcctcatt ggcacaacag gccgttccac
     2401 cgggcctcat ttacactttg aaattaacgc tcaaggcgta ccggttagtc cggcaacctg
     2461 gctcaaaaga gtctttcctt gagtaactta tgtataatta gaccgttaaa acaaaaagag
     2521 caacatctgt tgctctttcg tatgcgctga gagggagttg aaccctcacg ccttgcggca
     2581 cttggccctc aaccaagcct gtctgcctat tccagcacca gcgcatgaac cattattata
     2641 tactttgcgc gaaacttgtc aagatatcgc gccaaagagg tgactttgaa aacaacaatt
     2701 gtattagatg caatgggcag cgatgaccgc ccccaacccg aattaatcgg tgccattcag
     2761 gccagtgcca gtggcgacat aaacattatt ttagtgggtg atgaaaccat tctaaaaccc
     2821 gctcttgacg aattgcccgg cgacaaaaca catatcagca tcgtgcacgc acccgatgta
     2881 ctcgccatgg acgatgacat ccgccaggca cgctccaaaa agcaaaacac catggcagtt
     2941 ggaatgcagc tggtcaaaga tggcaaagca caagcctttg tgaccgcggg caacaccggc
     3001 atggcaatgt acttcggcac aaaaatcttt gggcttatca aaggggtcaa ccgaccctgt
     3061 ttgtgcgcca acttccccac cagaaacggg caatgtaccg ttttggatat cggtgccaac
     3121 gccgaatgca gacccgattt tttggtgcaa tttggccaaa tgggcgcggt ttacgcgcgc
     3181 ctgatgaaca atatcagcaa gccccgtgtc ggattaattt ccaacggaga agaagaacac
     3241 aagggcaatg atctcgtcaa aagcgcccac ccgctcatgc aaaagtcagt gccagggttc
     3301 atcggcaatg ttgaaggcaa agaaattttc ggcggtgaag tggatgtcgc cgtgaccgat
     3361 ggctttaccg gaaacgtctt tttaaaatcc accgaagccg tcgcgaaact gcttttgctc
     3421 accatcaagg atagtttgat gagtagcacg cgcactaaaa tagctggttt actggcaaaa
     3481 cctgctttca aaaaagtccg cagtttactt gaccccaacg aaatcggcgc cgcgccattg
     3541 ctggggctca acgcgctcgt ttttgtcggg catggccgtt caaactcgga tgccatcgtc
     3601 agcgcaatca atcaagcccg caaagctatt gaagctgata tgcttgttga attacaaaac
     3661 gccatcaaac gatagtggag aatcacaatg agactaatca ttaatgaaaa gaaaatcaaa
     3721 cgtaacaaga agatagctgg cgcgttcacc attgccagtt tggctgtgct gggcacgggt
     3781 ctcttttttg cctttaaatc ggatatgatt ttatggtctt atgtggccct aattgtcgga
     3841 tttgtactga cgcagattag tatgtactac tccagtcgct tttcgcgctc accccgctac
     3901 gacgaagtga tgcataaagc gctcgaaacc attcgcggtg attacagttt ttacgtctat
     3961 accaccacag tgcccaatct gcttttaggt ccaaacaaat tttacgcctt acatcctatc
     4021 gttacaccgg gcactattag ctacgtcaat ggcaagtgga gagaaaaagg tcgcggtatc
     4081 ttaatgcgca ttatcggtca ggaaaattta ggcaacccgg aaagagaact ggctattcag
     4141 attcaaggat tgcaggataa actcaacgaa aacggcctgg tatactcaga acaacccccg
     4201 attgtgccct tgttggtact tctcacagat aaaaccgtca tcggcgagat ggaagatgcg
     4261 cctattcagc acgtgaaaat caatgaactt aaacgcacta ttcgtcgttt cgatcgcgaa
     4321 gaccccgaag gggtcattcc agaagaaaaa atggaacaga ttaaatccat tttgggctaa
     4381 tctttattga cttcaactta aatatcaaaa aaatagattc aaaaaacgtt ctccgcggag
     4441 aacgtttttg tattttcaca actatgtttt taattcaatc ccacgtactt gcgcagcgta
     4501 gcgacttctt tatccgtcaa gtatcgccac tcccttggtt taagattgcc cagctttaac
     4561 gtaccaatgc gcacgcgttt aatctttcgc acgggcaagc ctatccgcaa accggtcagt
     4621 ctgatttggc gttttttacc ttcttttagc gtaatgcgca accaggcacc attatgcacc
     4681 ttgttgataa actccacgcc ggcaggcctg gtgcgatagc catcctctag aacaaccccc
     4741 cgacgccaaa tgctcaactg ctcttcatcc ggcatgtgat tgaccaaaac ttcatactct
     4801 ttttcatgtt cataacgggg atgagtcagg cggttagcca actcgccatc attcgtcatc
     4861 aaaaccaagc cttcgctgtc aaagtccaaa cgcccaacgg ggaaaagttg ctcgggcacg
     4921 tcaattaaat ccagaacggt cggacgtgga aaattctcat cgtaatccga aagataaccg
     4981 cggggtttgt agatggcaat gtaaaccttt ttttgtaaac cccgtaaaag tttgccatca
     5041 accataatgg catctttgtc ggcatctgct ttatcaccca gctttgccag tttgccatta
     5101 acgcgcactt ttccagcatc aataagaagc tcacacgcgc gacgcgaccc ataacccgcg
     5161 gctgcaagta ttttttgtaa tcgttcttct gtcattagtc ctttaaaatc tctgctactt
     5221 cttcgctctc ggctttgcct tgcaaaagtt catccaaatt aagttcgggt agttgctcta
     5281 agctttccaa accaaaatgt tgcaggaatt ctggtgtcac gccatataaa atcggtcttc
     5341 cggctgcttc tgaacgtccc aactcctcaa tcaagcctct ggaaagcaag gtgcgcacga
     5401 caccatcgct gtttacacca cgaatgctgt caatctccgg acgcgtagta ggctgtttat
     5461 aagcgataat cgccaacgct tccaatgcag cctggctcaa tcgtgtggtg
//
LOCUS       DAOSFC010000071         5432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13320_length_5432_cov_5.020829, whole genome shotgun sequence.
ACCESSION   DAOSFC010000071 DAOSFC010000000
VERSION     DAOSFC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13320_length_5432_cov_5.020829"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /gene="cbiR"
                     /locus_tag="PK791_02870"
     CDS             <1..506
                     /gene="cbiR"
                     /locus_tag="PK791_02870"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HOH92287.1"
                     /translation="KQSKADWLKKAELSLNQLSEWVGEPHLLAIENLDNYPVDFIDDV
                     FEGNTFSRCIDIGHLWLEGHDAVAYLEKHIHRARVLHIHGVAERDHKSLRHVPLEELK
                     RVLTVIHQSGFDGVMTIEVFNEEDFSGSLTQLNLALAQLGLSWQAQSGESAKPAKYHR
                     DLYESRG"
     gene            508..1059
                     /gene="cobU"
                     /locus_tag="PK791_02875"
     CDS             508..1059
                     /gene="cobU"
                     /locus_tag="PK791_02875"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOH92288.1"
                     /translation="MSAKKELVLVLGGARSGKSDWAQSQLETCTRDVLFVATATAGDE
                     EMRSRIQKHRASRPAHWQTLEAQLNVGQAIVAARPQGWVLLDCVTMLASNVLFSLPEP
                     VVEMDYQNAMQAEIEALIDAWHKQDASWMLISNEVGLGLVPPNALSRYYRDELGRANQ
                     RLAKVATKVVFMVAGIPMVIKEL"
     gene            complement(1131..2591)
                     /locus_tag="PK791_02880"
     CDS             complement(1131..2591)
                     /locus_tag="PK791_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92289.1"
                     /translation="MNTIENIPLEKTIALAMDEPVPNNGFKYRLRSELNSAVLRKSTQ
                     ARFFSLKLKPALITLTVVAAIALVFLNPKIVSGIRAFLSFIPGIGITQTEPGNYIVNP
                     QEATLMEGVSFQVSKGVATGQNITLLLEVKGLTEEMLFTSDEHSRFPEVVYSLEINQA
                     NTLELQEKSARWNGDSGYEIKLVFPATDKNLSRATLSLNHTPFANPDLSPNNITIPIV
                     FKEITDPDFALPVIDIQTTPYLPAPLTYSSKQTDENIPASPTQIREKTQFISTEDMYC
                     AEIHHMIQEQDGTILLGRIYWADESAKMPRFSPLSVELKDENGNIIQTSYFNTQDDIP
                     TSTDMWLPWSLKTTQPLVSGKYYLAFNGLTVRVFTDEKIVLPDSIISNSKQPGEYTLD
                     ININGIRMSLSEISFSKTTDGSHLSFIVHSDSQVESLTLFHEKLRNMDISQIDIKKFT
                     VNVGFSDVIDLKDGSLVITSIDFHLSEAIQLEFLIP"
     gene            complement(2588..3151)
                     /locus_tag="PK791_02885"
     CDS             complement(2588..3151)
                     /locus_tag="PK791_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH92290.1"
                     /translation="MQSDKQTADSTEINSEEEIVLLVKQDIRNFPLLYDRYVDQIYKY
                     FLSRVQNREIAEDLTSELFFTILKNFNKYKHKGYFRAWMYKIAQNLFYRQLQTPSQPA
                     IEDFEYVPSTKSLPEQNAIEKERISKVRQFILEDSDLNQEILRLRFVAELKFSEISML
                     IGKSESATKKHFYRLLERIKEFVENEK"
     gene            complement(3339..4601)
                     /locus_tag="PK791_02890"
     CDS             complement(3339..4601)
                     /locus_tag="PK791_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOH92291.1"
                     /translation="MKDTIKKKRPVWLSKFIFLLSILGPGLITASADNDAPGIATYSV
                     AGATYGYKLIWVILVITIGEVVILEMAARMGAVTGKGTADLIRERFGVKISTLAMLLL
                     IVANLATTVAQFAGVAAAGELFGISRYISVPIAAVVMTFIVLKAEYKYVERVLLVLCL
                     ASLSYVVAVFTVKPDWAAIGQAIIRPQIELSGDFLVTMLAVIGTTITPWAIYYLQASV
                     ADRGVDMKEYRYTRLDVSFGSAWGNVISAFILIVTAATLYVNGVAVNDAKDAALALQP
                     LAGEASSILFSVGLLGASLLAVSVLPLSTTYAFCEAYGFERGLNRKVKEAPVFYGMLI
                     AIMAISSLVVLIPNAPLFQMMILSQTLNAMLLPVLLILVLKLANSKEIMGKYTNSKLT
                     NILAIGITLLIVVVTIALFFEPLLNKIF"
     gene            complement(4617..>5432)
                     /locus_tag="PK791_02895"
     CDS             complement(4617..>5432)
                     /locus_tag="PK791_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH92292.1"
                     /translation="LNSQIIAWEDVASIEHDDPLRLKVSQERLVQMEPVDIAAILDSL
                     DHHTSKALLEGFDDEILADTLEQSSFEVQQAVLAAMEPERAADVLEEMDPDEAADLLA
                     ELDDEHSEQLLSLMEDEDEIEMRQLLAYPENSAGGIMTTEFAWVPGEFNVEQALTHLR
                     TSEEAKEDEFMYYLYILDHEERLEGVVSLRDLVTAPLEKSLHDWADDDVVHVEPLTHQ
                     NETAYLVAKYDLMAIPVIDPNNHKMLGIVTVDDALDTVLPTAWKKRLPRFAGK"
BASE COUNT         1433 a         1160 c         1293 g         1546 t
ORIGIN      
        1 gcaaacagag caaggcggat tggctgaaaa aggctgaact ctcgctcaat cagctgagcg
       61 aatgggttgg cgaaccacac cttttggcaa ttgagaacct tgataattat ccagttgatt
      121 ttatcgatga tgtttttgag ggaaatacgt ttagccgctg cattgatatc ggacatctct
      181 ggcttgaagg gcacgacgcg gtggcctatc tcgaaaaaca tattcaccgc gccagggttt
      241 tgcatattca tggcgtggcg gagcgtgacc ataagtcctt aagacatgtg ccgcttgaag
      301 agctcaagcg cgtattaact gttattcatc agtcgggttt tgatggcgtg atgacaatag
      361 aagttttcaa tgaagaagat tttagcggtt cgttaacgca gctcaatctc gctttggcac
      421 aactggggct tagctggcag gcgcaatcag gcgaaagtgc caaacccgcc aagtatcacc
      481 gcgaccttta cgaaagcagg ggctgaaatg agcgcgaaaa aagagctggt attggtgctg
      541 ggtggcgcgc gcagtggaaa atcggactgg gcgcaaagtc agcttgaaac ctgcacgcgg
      601 gacgtgcttt ttgtggcgac ggctaccgcg ggcgatgaag aaatgcgcag tcgcattcaa
      661 aaacaccgtg ccagccgacc cgcgcactgg caaacgcttg aagctcagct gaatgtggga
      721 caggcgattg tagcagccag gccgcagggc tgggttttgc tggattgtgt taccatgctg
      781 gcgagcaatg ttttgtttag cttgccggaa cccgttgttg agatggatta tcaaaatgcg
      841 atgcaggcag agattgaggc tttgattgat gcctggcaca agcaggacgc gagctggatg
      901 cttatctcca acgaagttgg tttggggctt gtgccaccca acgcgctttc gcgttactat
      961 cgtgatgagt tagggcgggc taatcaacgc ctggcaaaag tcgccacaaa agttgtcttt
     1021 atggtggcgg gcattccgat ggtaatcaaa gaactgtaaa aagtgcaagt gaagcaaatc
     1081 tcttgtacaa atcgtttgct tcacctgctt atacttatga caaacttttc ttagggtatc
     1141 aaaaactcta gctgtatcgc ttcacttaga tgaaagtcaa tgcttgtaat aaccaggctt
     1201 ccatctttga gatcaatgac atctgaaaag cctacattga ccgtgaattt ttttatgtct
     1261 atttgtgaaa tatccatgtt tcttagtttt tcgtggaaca aggtcaaact ttctacttga
     1321 ctgtcggagt gaacaatgaa tgacaaatgg cttccatcag ttgtttttga aaaactgatt
     1381 tctgacagag acattcttat cccattgata ttgatatcaa gtgtatattc tccaggttgc
     1441 ttagaattag agatgattga atcgggtaaa acgatttttt catctgtaaa cactcgcacg
     1501 gttagtccat tgaaagcaag gtaatattta ccgctaacca aaggctgagt agtctttaag
     1561 ctccatggca accacatatc ggtgcttgtt ggtatatcat cctgtgtgtt gaaatagctt
     1621 gtttgaataa tattcccatt ttcatctttc aattcaactg aaagaggtga aaaccgcggc
     1681 attttcgctg attcatctgc ccaatatatg cgacctaata gaattgtgcc atcctgttct
     1741 tgtatcatgt gatgaatttc cgcgcaatac atatcttctg tactgataaa ttgtgttttc
     1801 tcacggattt gtgttggtga agctggaata ttctcatcgg tttgcttgct tgaataggtc
     1861 aacggggctg gaagatatgg ggtcgtttga atatcaataa ctggtagtgc aaaatcaggg
     1921 tcagttattt ctttaaaaac aatcggtata gtaatgttat ttgggcttaa atcaggatta
     1981 gcaaacggtg tgtgattcaa acttaaggtt gctctactta agtttttatc tgttgctgga
     2041 aaaacaagtt ttatttcgta gcctgaatca ccattccaac gggcactctt ttcttgcaat
     2101 tccaatgtgt ttgcttggtt gatttcaagc gaatagacaa cttcaggaaa tctggagtgt
     2161 tcatctgatg tgaagagcat ttcttctgtc agccctttta cttccaggag caaagtaatg
     2221 ttttgacccg tagcgacacc tttagagacc tgaaaagaga cgccttccat taatgttgct
     2281 tcttggggat taacgatata gttgccaggt tctgtttgtg ttatgcctat gcctggtata
     2341 aaagaaagaa acgcgcgaat gccactaacg attttcggat ttaagaaaac caaggcgata
     2401 gctgctacaa cggtcagagt tattaaggct ggttttaatt ttagagagaa aaatcttgct
     2461 tgtgtacttt ttctcaaaac agctgaattt aactctgatc tcaaacgata tttgaatcca
     2521 ttatttggta cgggttcatc catagccaaa gcaatagttt tttctaatgg tatgttttca
     2581 attgtgttca tttttcattc tccacaaatt cctttattct ttctaaaagc cgatagaaat
     2641 gcttttttgt ggctgattca gattttccaa taagcatgct tatttctgaa aactttaatt
     2701 ccgcaacgaa gcgtagtctc aaaatttctt gatttaaatc tgaatcctcc aggataaatt
     2761 gccgaacttt agaaattctt tccttttcaa tagcattttg ttcaggaaga gattttgttg
     2821 aaggtacata ttcgaaatct tcaatggcag gttgagatgg cgtttgcaat tgtctgtaaa
     2881 acagattttg tgcaatttta tacatccaag cgcggaaata acctttgtgc ttatatttat
     2941 taaagttctt taatatagtg aaaaacagtt cagaagttaa atcttcagcg atttcgcggt
     3001 tttgaactct ggataaaaag tatttataaa tttgatcaac atatctgtca tagagcaaag
     3061 gaaagttcct aatatcttgt ttaaccaaca atactatttc ctcttcactg ttgatttctg
     3121 ttgaatctgc tgtttgttta tcagactgca ttctcactcc tgtcgaggta cctcacttaa
     3181 ttaaagacga aaatttctga aaagtgacaa agagaacaaa ttattcagtg cttttttcgc
     3241 agacaatcct tttttgagca tcaattagtt ttatttactt tttaaaacac aaatccccag
     3301 catgaaactc tggggattta tcgttacagc cagcttgtct aaaatatctt gttcaacaat
     3361 ggctcaaaga agagcgcgat ggtgacgaca acaatcaata gcgttatgcc gatcgccagg
     3421 atgttggtca gtttggagtt ggtgtattta cccataattt ctttgctgtt agcgagttta
     3481 agtaccagga tgagtaaaac gggcaaaagc atggcgttca gggtttgcga aagaatcatc
     3541 atttggaaga gcggggcgtt tggaatgagt accaccagtg agctgatagc cataatcgca
     3601 atgagcatgc catagaatac aggcgcttct ttcaccttgc ggttgagccc gcgttcaaag
     3661 ccgtatgctt cgcaaaaggc gtaggtggtt gacaggggca aaaccgagac agccaacaac
     3721 gacgcgccca ggagcccaac cgagaacaaa atgcttgaag cttccccagc caaaggttgc
     3781 agtgccaggg cagcatcttt ggcgtcgttc accgctacgc cgttcacata gagcgtggct
     3841 gcggtaacga tgaggataaa ggctgagatg acattgcccc aggcagaacc aaagctgaca
     3901 tccaggcgtg tgtagcgata ttctttcata tcaacaccgc ggtcagctac cgaggcttgc
     3961 aggtagtaaa tggcccaggg ggtgatggta gtaccgatga cagccagcat cgtgaccagg
     4021 aaatctcccg aaagttcaat ttgggggcgg atgatggctt gtccgatggc tgcccagtcg
     4081 ggtttgacgg tgaaaacagc cacgacataa ctgagcgacg ccaaacacaa aaccaaaagc
     4141 acacgctcga catacttata ttccgctttg agcacgataa aagtcatcac aaccgcggca
     4201 atcggtacgc taatgtagcg actgatgcca aagagttcac ccgccgcggc aacaccggcg
     4261 aactgtgcga cggtggtggc aaggttagca acgattaaca gcaacatggc cagggtggaa
     4321 atcttcacac cgaagcgttc gcggataagg tccgcggtgc ctttaccggt cacggcaccc
     4381 atgcgcgctg ccatctcaag gatgaccact tcgccgattg taatcacgag gataacccaa
     4441 atcagtttgt agccataggt cgcgccagcc actgaatagg ttgcgatgcc aggtgcgtcg
     4501 ttatctgcac tggcggttat taaacccgga ccaagaatgc tgagcaagaa aatgaatttg
     4561 ctaagccata ccgggcgttt tttcttgata gtgtccttca ttttatgctc ctttcgttat
     4621 tttcctgcga atctgggcag gcgtttcttc cacgctgtgg gcaggacggt gtccagcgcg
     4681 tcatcaacgg tgacgatccc cagcatttta tggttatttg ggtcgatgac agggattgcc
     4741 atgaggtcat acttggcaac caggtaagcc gtttcatttt gatgggtcag gggttcaacg
     4801 tgcacaacat catcatcagc ccagtcatgg agtgactttt caagtggcgc ggtaacgaga
     4861 tcgcgcaagc tcaccacgcc ttccagtctc tcttcgtggt ctaaaatata cagatagtac
     4921 atgaactcat cttctttggc ttcttcggat gtacgcaggt gggtgagggc ttgctccacg
     4981 ttgaactcgc ccggaaccca ggcaaactcg gtggtcatga tgccgcccgc gctgttttcg
     5041 gggtaagcca gcagttggcg catctcaatt tcgtcttcgt cttccatcag gctcagcaat
     5101 tgctcgctgt gttcgtcgtc gagttccgcc aaaaggtccg cggcttcgtc aggatccatt
     5161 tcctcaagca cgtccgcggc gcgttccggt tccatggctg ccaaaacggc ttgttgcact
     5221 tcaaaagagc tttgttcgag ggtatcggca aggatttcat catcaaaacc ttccagcaag
     5281 gctttgctgg tgtggtggtc taaactgtca agaatggcag cgatatccac gggctccatt
     5341 tgcaccagac gttcctggga tactttaagg cgtaaagggt catcatgttc gatgctggca
     5401 acgtcttccc aggcgatgat ttgactattg ag
//
LOCUS       DAOSFC010000072         5406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13415_length_5406_cov_4.834237, whole genome shotgun sequence.
ACCESSION   DAOSFC010000072 DAOSFC010000000
VERSION     DAOSFC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13415_length_5406_cov_4.834237"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..1611)
                     /locus_tag="PK791_02900"
     CDS             complement(244..1611)
                     /locus_tag="PK791_02900"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOH92293.1"
                     /translation="MARTSVPCPNCKTPITVDLIRLFDTNTNPNAKQILLSGQANYYQ
                     CPSCRSQGIYPTPIVYHDPEKELLLSYFPPELGAPVNEQERIIGPLIKKAVDDLPPEK
                     RKAYIFSPRTMLTKELLFETILESDGITPEMLKAQQEQLAFIQRLLMSSPDALPEILA
                     KDDERVTEEFFILLSRLVQTSLAAGDQQGAQALAMVQSVALEHTTVGKKIAQDSAEAQ
                     EVMNKLQELSKEGITREKLLDLAIESAESKVKLTMLVSAARGGMDYQFFQLLTEKAEQ
                     AQGDEKARFEGLRDSILELTEEIDKAQKVLYAEAKEMVDSLLEAENLPEATQQALPSF
                     TQATLEVLNDEFVKAQEAGDEERVKKIVTVLTVFRSANESSVYLELIDILMQAPDDAT
                     RDQVLEEAHEGLNDDFFQVLGSLINQVEQSGEQPELLAKLREVNSAAQKFIMRKNFEA
                     ASNES"
     gene            1826..3403
                     /locus_tag="PK791_02905"
     CDS             1826..3403
                     /locus_tag="PK791_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOH92294.1"
                     /translation="MSFTLTYASMANPPDIFHTSYETALAELKTKLGKDYPLIIGDEK
                     RFKEEKFPALSPINRDWTLASFQSGDVQDAQDAVAAAKAAFPAWSKLPWQERVAYVRK
                     FAQAIQDRIFEIGAAVSLDVGKNRMEALADVQEAVDLLLWPCKMMEQADGYQVKLAND
                     PMEGVLSVNESHMVPHGVWLVITPFNFPAALACGPMGNALVAGNTVVLKPASDSAWAT
                     AMMMECAEKAGFPSGVINYLTGPGGTLGNALIDHPDIAGVTFTGSAAVGLGINRKFAE
                     FKYPRPVVLELGGKNATIVSKNADIADAALGCMRSAFGLQGQKCSANSRIFVEREVYQ
                     AFVEELVRLTKEIKIGDPTMRENWFGTVINERAAADYEKYIEEFETVGRILTGGKRLT
                     DGDLAKGLFCAPTIVVDVPMTHYLWKHEMFLPITMVTAVDSLEEAMGYANDTDYGLTA
                     GFMGSAEEGEWFLDNIQAGVCYVNRVMGSTTGAWPGFQPFGGWKASGSTGRNTGGPYA
                     LLSYMREQSRTRVSIIE"
     gene            3498..4535
                     /locus_tag="PK791_02910"
     CDS             3498..4535
                     /locus_tag="PK791_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOH92295.1"
                     /translation="MSQRNKNRIIELTSLINLSDAQIAMINDGIENVRISTFPRSTAE
                     QIPAEQWAKTEVLLSAGDYLPTREMAPKLKWIQISFAGVDPILNRAIIHDENIQVTSA
                     SGVMVSQMGEYILMALLMLGHKMPAIINAQLKNEWISNKSGMVMPKELRGSTVGIIGY
                     GSIGRELARLLYHFGAKVLATKADLSNLEDTGYIPEGLGDPHGDYFYRMYPTDALLGM
                     LPECDFVVLTAPLTEKTRNMFSTEAFAAMKPSAYFINVSRGALVDESALVVALRNKTI
                     AGAMLDVFNEEPLPANSPLWKQPNLIITPHVSGVSPKILDNVLRFFNTNLKRYRDDLP
                     LYNVIDPKRGY"
     gene            4536..>5406
                     /locus_tag="PK791_02915"
     CDS             4536..>5406
                     /locus_tag="PK791_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOH92296.1"
                     /translation="MDLFDSSLEEQLRKHAPLAARMRPQTLDDIVGQDHIIGKGSLLR
                     RVIEADRLHGSIILYGPPGTGKTSLAQVIANSTKAHFEQLSAVLAGVADLRKAIEAAR
                     ERRRLYQTRTILFIDEVHRWNKAQQDALLPHVENGLITLIGATTQNPYFDVIKALVSR
                     SRVFELNSLTVENIKEILLRAITDEERGFGLRKVKIDDDALQHLAEISSGDARNALNA
                     LELAVESTPLGQEETHITLEIAQESIQRRAVLYDKTDDQHYDTISAFIKSVRGSDPDA
                     ALYWLAKMLYAGED"
BASE COUNT         1341 a         1522 c         1284 g         1259 t
ORIGIN      
        1 ccaaaccaca aaaacgcaag gttttcatgc tctaaattgt acccgagaat ggcaaacctg
       61 cgcagacaag gcccaggaag gcattttgga tggtcggact tgtaaaaaag ccaagttctc
      121 acccatgacc acgagaagaa aaaagcctgc ttaaaacagg cttttggttt aatatgattt
      181 attctgcttc tgtgcctgct cactacatat cgtagtgtgg catagcaatt cacttataaa
      241 cgcctaagat tcgttggatg cggcttcaaa gttcttgcgc atgatgaatt tttgcgcagc
      301 gctgttgact tcgcgcaact tcgcgagcaa ctcgggctgc tcaccacttt gctcaacctg
      361 attaatcaag cttcccagca cctggaaaaa gtcatcgttt aagccttcgt gcgcttcttc
      421 caaaacctgg tcgcgtgtag catcatcggg tgcctgcatc agaatatcaa tcagctccag
      481 ataaacactg ctctcgttcg ccgaacggaa aacggttaaa acggtcacga tcttcttaac
      541 ccgttcttca tcaccagctt cctgggcttt tacaaactca tcgttcagca cttccagcgt
      601 cgcctgcgtg aaagatggca gggcttgctg ggttgcttca ggtagatttt cagcttcaag
      661 caagctatcg accatttcct tggcttcagc atataaaacc ttttgggcct tgtcgatttc
      721 ttctgtcagt tccaaaatgc tgtcgcgcaa accttcaaac ctggcttttt catcaccctg
      781 ggcttgttca gccttttcgg tcaataactg gaagaactga tagtccatgc caccccgtgc
      841 cgcgctgacc agcatggtca gttttacctt cgattcagcc gactcaatcg ccaaatccaa
      901 aagcttttcg cgggtaatgc cttctttact caactcctgc aatttgttca tcacttcctg
      961 ggcttcagca gagtcttgcg cgattttctt gccaaccgtg gtatgttcca gtgccacact
     1021 ttgtaccatc gccagggctt gcgcgccctg ttggtcacca gccgccagcg atgtctgcac
     1081 caggcgtgaa agcagaatga aaaactcttc cgtcacgcgt tcatcgtcct ttgccagtat
     1141 ttcgggcaag gcgtcaggtg atgacatcag caaacgttga ataaacgcca actgctcttg
     1201 ttgcgctttg agcatctcgg gtgtaatacc atccgattcc agaatagtct cgaaaagtaa
     1261 ctctttggta agcatggtgc gcgggctaaa gatataagct ttgcgctttt cgggtggcag
     1321 gtcatcaacc gcttttttaa ttagcggacc aatgatgcgt tcctgttcgt tcacaggcgc
     1381 gccaagttcg ggtgggaaat aactcagcag taactctttt tcggggtcat gatagacaat
     1441 cggcgtggga taaataccct gactgcgaca gctggggcac tggtaatagt tcgcctgccc
     1501 ggaaagtaaa atttgcttgg cattcgggtt ggtgttggta tcaaataacc ggatcaaatc
     1561 gacggttatc ggggttttac aatttgggca cggtacactt gttctggcca tagtttctct
     1621 tttccttatc gttaatgtcc ccattataac agtcgatagt taaaaaattt cttaacattc
     1681 caacttaaat actacggaaa gatgctatac cgcacttctt tatatataga caggcaaatt
     1741 tgtggactgc aatcccagat tctcgcgtca attctgatag aatagagatt aagaaaatta
     1801 aatcattcac gcacaggagg ctccaatgtc atttacactt acctatgcca gcatggccaa
     1861 tccacctgat atttttcaca catcctacga aactgcgctg gcagaactca aaaccaaact
     1921 tggcaaagac tatccgctga ttatcggtga tgaaaaacgc tttaaagaag aaaaatttcc
     1981 ggcgctttca cccatcaaca gggactggac actcgccagc ttccaaagtg gcgatgtcca
     2041 ggacgcgcaa gatgccgtcg cggccgccaa agccgctttc cctgcctgga gtaaactgcc
     2101 ctggcaggaa cgtgttgctt acgtgcgcaa atttgcacag gcgattcaag accgcatctt
     2161 cgaaatcggc gccgcggtca gtctggacgt tggtaaaaac cgcatggaag ctctggcgga
     2221 cgtgcaggaa gccgttgacc tcttgctctg gccctgcaag atgatggaac aagctgatgg
     2281 ctaccaggtg aaactcgcca acgaccccat ggaaggcgtc ctgtcagtca acgaaagcca
     2341 catggtgccc catggcgtgt ggctggtgat tacacccttc aacttccccg ccgctttggc
     2401 ttgcggaccc atgggtaacg ccttggttgc cggcaacacg gtggtgctaa agccagcctc
     2461 tgattccgcg tgggcaaccg ccatgatgat ggaatgcgcg gaaaaagccg gcttccccag
     2521 tggcgtcatc aattacctta ctggcccagg tggcaccctt ggcaacgcgc tcatcgacca
     2581 ccccgatatc gctggcgtta cctttaccgg ttcagccgcg gttggcttgg gtatcaaccg
     2641 caaatttgcc gagttcaaat acccccgtcc ggtcgtgctc gaattaggcg gtaaaaacgc
     2701 cactatcgtc tccaaaaacg ctgatatcgc cgatgccgct ttaggctgta tgcgctccgc
     2761 gtttggcttg caaggtcaaa aatgctccgc caactcgcgc atctttgttg aacgtgaagt
     2821 ttatcaagcc tttgttgaag aactcgtgcg cctgacaaaa gaaatcaaaa tcggtgaccc
     2881 aaccatgcgc gaaaactggt ttggaacggt tatcaacgag agagccgcag cagattacga
     2941 aaaatatatt gaagaattcg agactgtggg tcgcattttg accggcggaa aacgcctcac
     3001 cgatggtgac ctggcaaaag gcctcttctg cgcgcccacc atcgtggttg atgtacccat
     3061 gacgcattac ctgtggaaac acgaaatgtt cctgcctatc accatggtca ccgccgttga
     3121 cagccttgaa gaagccatgg gctacgccaa cgacaccgac tatggactta ccgccggctt
     3181 tatgggcagt gcggaagaag gcgaatggtt ccttgacaac attcaggctg gcgtgtgtta
     3241 cgtcaaccgt gttatgggca gcaccaccgg cgcgtggcct ggttttcaac cctttggcgg
     3301 ctggaaagct tccggctcta ccggtcgcaa cacgggcgga ccctacgccc tgctctcgta
     3361 catgcgcgag caaagccgca cacgtgtgag tataattgag taaacacccc taagaatact
     3421 atggctggcg gccaccgaca tttgcgagtc gccgccagct ttttgtttgg attttacaga
     3481 ccttttgcgg agaaaacatg agccaacgaa ataagaacag aattatcgaa cttaccagcc
     3541 tgattaacct gagcgatgcc caaatcgcga tgattaacga cggcattgag aacgtgcgca
     3601 tcagcacctt tccacgaagc accgccgagc aaattcctgc tgaacagtgg gcgaaaaccg
     3661 aagttttgct ctcggcaggc gattatttgc ccacccgcga aatggcgccc aaactcaaat
     3721 ggatccaaat ctcattcgcc ggcgttgacc caatcctgaa ccgtgccatc atccacgatg
     3781 agaacattca agtcacttcc gcgagtggtg tcatggtcag ccaaatgggc gaatatattc
     3841 tcatggcact gctcatgctg ggtcacaaaa tgcctgccat catcaatgcc cagctcaaga
     3901 acgaatggat tagcaacaaa tccggtatgg ttatgcccaa agagttgcgt ggcagcacag
     3961 taggcatcat cggctatggc tcgatcgggc gtgaactggc ccgtttgctc taccactttg
     4021 gcgcgaaagt gttggcaact aaagcggatc taagcaatct ggaagatacc ggctatatcc
     4081 ccgaaggact gggcgacccc cacggcgatt acttctaccg catgtacccc accgacgcgc
     4141 ttttgggcat gttgcctgaa tgcgacttcg tggtccttac cgcgccactc accgagaaaa
     4201 cccgcaatat gttctccacc gaagccttcg ccgccatgaa accaagcgct tatttcatca
     4261 acgtctcgcg gggcgcgctg gtggatgaat ccgcgctggt tgtcgcttta cgcaacaaaa
     4321 ccatcgcggg cgcgatgctg gatgtcttta acgaagaacc cctgcccgcc aacagtccac
     4381 tttggaaaca gccgaaccta atcatcacgc cccacgtatc cggcgtttcg cccaaaatac
     4441 tggacaacgt cctgcgcttt ttcaacacca acctaaaacg ctaccgggat gacttaccgc
     4501 tttacaacgt cattgaccct aaaagaggat actaagtgga cctttttgat tcatccctgg
     4561 aagagcaact gcgcaaacac gccccgctcg ccgcgcggat gcgtccccaa acgctggacg
     4621 acatcgtcgg ccaggatcac atcatcggca aaggcagcct gctcaggcgt gtgattgaag
     4681 ccgaccgctt gcatggctcc atcatccttt atggtccacc cggaaccggc aaaaccagcc
     4741 tggcgcaggt catcgccaac agcaccaaag cccattttga acagctcagc gccgttttgg
     4801 cgggtgtggc tgatttgcgc aaagcgattg aagccgcccg cgaacgccgc cgcctttacc
     4861 aaacgcgcac cattctgttc atcgacgaag tgcaccgctg gaacaaagcc cagcaggacg
     4921 ccctcttacc gcacgttgaa aatgggctca tcacgctaat cggcgctacc acccaaaacc
     4981 cctattttga cgtcatcaaa gccctggtca gccgctcgcg cgtttttgaa ctcaacagcc
     5041 tgaccgttga gaacatcaag gaaattttgc tacgcgcaat aaccgatgag gaacgcggat
     5101 ttggtctgcg aaaggtcaaa atcgacgatg acgcgctgca acatctggcg gaaatcagca
     5161 gcggcgacgc gcgcaacgcc ctaaacgcgc tggaactcgc cgttgaaagc accccactcg
     5221 gacaggaaga aacccacatc acgcttgaaa tcgcccagga gtccattcag cgccgcgctg
     5281 tgctttacga taaaaccgat gaccaacatt acgataccat ctccgcgttc atcaaatcgg
     5341 tacgtggctc tgaccccgac gccgctctct actggcttgc taaaatgctc tatgccggcg
     5401 aagacc
//
LOCUS       DAOSFC010000073         5399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13437_length_5399_cov_5.416729, whole genome shotgun sequence.
ACCESSION   DAOSFC010000073 DAOSFC010000000
VERSION     DAOSFC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13437_length_5399_cov_5.416729"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="selD"
                     /locus_tag="PK791_02920"
     CDS             <1..726
                     /gene="selD"
                     /locus_tag="PK791_02920"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOH92297.1"
                     /translation="ADLPVELLRDIMRGAADKCHEAGVVIAGGHTVTDPEPKFGLAVL
                     GLVNPADILTKKGLQAGDALFLSKPLGIGVSTTALKRGKLGPDALQTITEWMMRLNKD
                     AAELAEACDLQAVTDITGFGLMGHASEMAKASDVGLEFWYDHLPIHDCAQACIEAFTY
                     PGGAFNNRLHFGKNVFIDPELSENQHMVIFDPQTSGGLLMGVPAEKVSRFKALANKRG
                     VDVWEIGRVLAENEYRLLSQKPV"
     gene            complement(822..1676)
                     /locus_tag="PK791_02925"
     CDS             complement(822..1676)
                     /locus_tag="PK791_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOH92298.1"
                     /translation="MACEKNLSHGVGGNSFENIRKTTHFAISAHQDDIEIMAFHGILA
                     CYQQEHCRFGACVVTDGRGTPRAGEFADVSDDEMVEIRAAEQTKAAEIGDYANCVLLG
                     HRSSDVRQLDFEPLTSELVRLLNLAQPKVIYTHNLADKHKTHLAVGLHVIKAIRRLPK
                     KLHPEKLYGCEVWRSLDFLPDTHKVLLDVSARPELQSALINAFRSQIAGGKNYEQAVI
                     GRRLANATFQDPYAPDAHSRLSIAMDMTPLILDDQLCPRAFMREILRATRDEIDGNLA
                     SFLGDLTV"
     gene            complement(1686..2741)
                     /locus_tag="PK791_02930"
     CDS             complement(1686..2741)
                     /locus_tag="PK791_02930"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOH92299.1"
                     /translation="MGLHRKKNKAGKSRYTQKELEFMLYVNTGNSHDPSLNLAAEEYV
                     IRNIAPKDEILLFYINGPSIIIGRNQNTIQEINTDYVEAHNIKVVRRLSGGGAVYHDE
                     GNLNFSFIAPYSKDNFTNFKKFTEPVIKVLNEMGVPAELRGRNDIVVNGQKISGNAQY
                     VAGERMVSHGTLLWQTDLGRVGEALRVKAAKIESKGIKSVRSRVANISEFIQGGLMSI
                     EAFRSRIIEGVFEGGDVREYVLTDDDWQAIQQLAQERYRNWDWNYGRSPAFDIRRETR
                     YPGGEIEVYLDVVQGVIKDIKFYGDFFGQRSVDELEKALTNVQYTPPAIAKALENMLI
                     SDYFVNFSLEELISLLY"
     gene            complement(2716..3867)
                     /gene="alr"
                     /locus_tag="PK791_02935"
     CDS             complement(2716..3867)
                     /gene="alr"
                     /locus_tag="PK791_02935"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOH92300.1"
                     /translation="MLYQTHVKIHLDNIRDNLLAIRERVGPDVKILPALKANAYGHGS
                     VEVARVAEDCGMDWIGVATVPEGMAIRQAGVRLPILKLSPAFPEEMEAAIENDVTLSV
                     IDAEYIQILQETAKRMGKKINVHLKVDTGMGRIGVRPETAPERALFIEKNCPNLHLQG
                     IFTHMPVADMSDLDPFLYTEDQIQHFKNVVETVNQAIGREIELPHCANSGDILGHKNA
                     FMKMVRPGIMIYGYYPDLSTPKTIFLKPGMSFYSRVSFVKKVKAGTPISYGQTWLAPR
                     DTFIGTVPVGYADGYNRQFSNRGRMLINGRSYPVAGRVCMDQTMIDLGPETDVKVGDQ
                     VVLLGKSGDLEITAYEWAEKLKTITYEITSQINQRVKRYYFDGSSQEEK"
     gene            complement(4017..5381)
                     /locus_tag="PK791_02940"
     CDS             complement(4017..5381)
                     /locus_tag="PK791_02940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOH92301.1"
                     /translation="MRVVVIGTGYVGLTTATVLAYIGHSVTCLDTNAEKINRLKQGIL
                     PIHEPHLDEVFDQVRHKMRFTTDYAQADIPNTEVIIIAVGTPSLPDGDADLTYLTQAA
                     ESLAQNLGEDFTLIVNKSTVPIGTGKWLEDRIHDFTDALDDRPGKRDFNVISSPEFLS
                     QGSALRDSFYPNRIIIGTQHAPSIERFSKLFEPIHKQNFVSPKCLPRPEGLQEVPIFV
                     CDVISAETIKYAANAFLATKISFINEMGYLAQKVGADVDALAKGMGLDPRIGSRFLNA
                     GIGWGGSCFGKDTAALVATARKNGLEMRIVQAARDVNYSLRQWIVNALQEKIGDLNGR
                     RVTLLGFSFKPNTDDLRDAPSIDISKELIKRGAIVTAHDPVALKNARLYYHDLGVVYE
                     DDLSKALENPEAVLLLTEWQDYLHIDWQNVPQCVIIDGRNHLDHTRLRDLGFETMRFG
                     SKQS"
BASE COUNT         1323 a         1344 c         1322 g         1410 t
ORIGIN      
        1 gcggatttgc ccgttgaact tttgcgagat attatgcgtg gcgcggcgga taaatgtcat
       61 gaggcgggtg tagttatcgc tggtggacat accgtaaccg atcccgagcc taaatttggt
      121 ttggcggttt taggtttggt gaatcctgcg gatattctca ctaaaaaggg cttgcaggct
      181 ggcgacgcgc tgtttttgag caaacccctg ggcatcgggg taagtacaac cgccctaaaa
      241 cgtggcaagt taggcccaga tgccctgcaa acgattactg aatggatgat gcgcctgaac
      301 aaagacgcgg cagaactggc agaagcttgt gatttgcagg cggtgaccga catcactggc
      361 tttggcctca tggggcatgc ctcagaaatg gctaaggctt ctgacgttgg gctggagttt
      421 tggtatgacc atttacccat ccacgactgc gcgcaagctt gcatcgaagc tttcacctac
      481 cctggtggcg cgtttaacaa tcggcttcat tttggcaaga atgtttttat tgaccctgaa
      541 ctgagcgaaa accaacacat ggtcattttt gatccgcaaa ccagtggcgg cttgttgatg
      601 ggtgtgcctg ctgaaaaagt ttcgcggttc aaggctctgg caaacaaacg cggtgtcgat
      661 gtctgggaaa tcggcagggt tttggcagaa aacgagtacc gcctgctgtc tcaaaagccg
      721 gtttagtttt tcaatagtca tgtaatagcg tcggcgagac gcctccgcga acggaaaaac
      781 attgctttta actgtggtct tttgaccacg gcttattcag cttagacagt caaatcaccc
      841 aaaaaactgg caaggttgcc atcaatctcg tccctggtcg cgcggagaat ctcccgcata
      901 aacgcgcgcg ggcagagttg atcatccaaa atgagcggcg tcatatccat agcgatgctc
      961 aaccgcgaat gagcgtctgg cgcgtaaggg tcttgaaaag tggcattcgc gagacgacgc
     1021 ccaatgacag cttgttcgta attctttccc cccgcaattt gtgagcgaaa ggcgttaatc
     1081 agcgccgatt gtaattccgg tcgcgcggaa acatccaaaa gcactttatg cgtgtccggc
     1141 agaaaatcca aagagcgcca tacctcacag ccatagagct tttcaggatg cagtttcttg
     1201 ggcaatcgcc gaatagcttt gataacgtgt aagccaaccg ccaggtgcgt tttgtgcttg
     1261 tcagccagat tgtgcgtgta aattaccttg ggctgagcca aattaagcaa cctgacaagt
     1321 tcgctggtca atggctcaaa atccagttgg cgcacgtcgc tactccggtg ccctaaaagg
     1381 acgcagttgg cgtaatcgcc aatttcagcg gctttggtct gttctgctgc gcggatttca
     1441 accatctcat catcactcac gtccgcaaac tcacccgctc gtggcgtgcc ccgtccatca
     1501 gtaacaacac aggccccaaa acgacagtgt tcctgttggt aacaagccaa aatgccatgg
     1561 aaggccatga tttcaatatc atcctggtgg gcgctaatcg caaaatgcgt cgttttgcgg
     1621 atattctcaa aagagttacc gcctacacca tgactcaagt ttttctcaca agccatcatg
     1681 cccccctaat acaaaagact gataagttct tcaaggctga agttcacaaa ataatcgcta
     1741 atgagcatat tctcaagcgc tttggcgatg gctggtggcg tgtattgaac attcgtgaga
     1801 gccttctcaa gttcgtccac gctgcgctgg ccaaaaaaat cgccatagaa cttgatgtcc
     1861 ttaatcacgc cctggacgac atcaagataa acctcaattt cgccacctgg gtagcgggtc
     1921 tcgcgccgga tatcaaaagc cggcgaacga ccgtagttcc agtcccagtt acgatagcgt
     1981 tcctgggcga gttgctgaat agcttgccag tcatcatcgg tcaacacata ttcacgcaca
     2041 tcgccacctt caaaaacgcc ctcaataatc cgcgaacgga aagcctcaat gctcatcaag
     2101 ccaccctgga taaattcgct gatgttggca acacgtgaac gaaccgattt aatgcctttg
     2161 ctctcaatct tcgcggcttt tacgcgcagt gcctcgccta ccctgcctaa gtcagtctgc
     2221 cacaaaagcg tgccgtggct caccatgcgc tcaccagcga catattgcgc gttaccggag
     2281 attttttgcc cgttgaccac aatgtcgttt cgcccgcgca gttcggcagg cacgcccatc
     2341 tcattgagca ccttgatgac tggctcagtg aattttttaa agttggtgaa gttatctttg
     2401 gagtaaggcg cgatgaacga aaaattgaga ttgccctcat cgtgatacac cgccccacca
     2461 cccgaaagcc gacgcaccac tttgatattg tgcgcttcaa cgtagtcagt attgatttcc
     2521 tggatagtgt tttggttgcg tccgatgata atcgatggcc cattaatata gaagagcagt
     2581 atttcatctt tgggcgcgat attgcgtatt acatattctt cagcagcaag gtttaaactg
     2641 ggatcatggc tgttaccggt gttgacatag agcataaatt caagttcctt ttgtgtgtag
     2701 cgtgactttc cagctttatt tttcttcctg tgaagaccca tcaaaatagt aacgctttac
     2761 acgctgattg atttgcgagg tgatttcgta ggtaatggtc tttaattttt ctgcccactc
     2821 atatgctgtg atttccaaat cgccactttt gccgagcagg accacctgat cgccaacttt
     2881 cacatcggtt tcggggccta aatcaatcat tgtttgatcc atacaaaccc gacccgcgac
     2941 aggatatgaa cgcccgttaa tcagcattcg cccgcgattg ctgaactgac ggttgtaacc
     3001 gtcggcataa ccaaccggaa ccgtgccaat aaaggtatct cgcggagcaa gccaggtttg
     3061 accatagcta atgggcgtgc ctgctttgac ctttttaacg aaacttaccc ggctgtaaaa
     3121 actcatgcct ggtttcaaaa agatggtttt aggtgtgctc agatcaggat aatagccata
     3181 aatcatgata cctgggcgca ccatcttcat aaaagcgttt ttatgaccaa gaatgtcacc
     3241 ggaattagcg caatggggca gttcgatttc ccgtccgata gcctggttta cggtttccac
     3301 gacgtttttg aagtgctgaa tttggtcttc tgtgtataaa aaggggtcca aatcgctcat
     3361 atcagcaaca ggcatatggg tgaaaatgcc ttggagatgc agatttgggc agtttttctc
     3421 aataaacaag gcacgttctg gcgcggtttc cggtcgcacg cctatacgcc ccatgccggt
     3481 atcgacctta agatgcacat tgattttctt tcccattctt ttggcagttt cctgcaaaat
     3541 ttgaatgtat tcggcatcga tgacgctgag cgtgacatcg ttttcaatcg cggcttccat
     3601 ttcttccggg aaagcggggg ataatttaag gataggcaag cgaaccccag cctggcggat
     3661 agccattcct tcgggaacag tggctacccc aatccaatcc atgccgcagt cttcagccac
     3721 ccgcgcgact tccaccgagc catgcccata agcattggct tttagcgctg gcaaaatttt
     3781 cacatccggc cccacccttt cgcggatggc aagcaaatta tcgcggatat tgtccaaatg
     3841 aatttttacg tgggtttgat agagcatttt tgaggctttc ttggctttta tctgttatat
     3901 agactgtatt agtccgcgtt cgattatacc aagtccctat tagactggca catagacata
     3961 gcaaagtaag agaatatgca tccgcgcttg gtgaaaaaga ccacgacacc cggggtttag
     4021 ctttgtttag aaccgaaacg catggtttca aagcccaaat ctctcaaacg cgtatggtcc
     4081 agatgattac ggccatcgat gatcacgcat tgaggcacgt tttgccagtc gatatgcaaa
     4141 taatcctgcc attcggtgag cagtaaaacc gcctcggggt tttcaagcgc tttgctcaaa
     4201 tcgtcttcat aaacgacacc caagtcatgg taataaagcc gcgcgttttt gagcgcgacg
     4261 gggtcatgcg cggtaacgat ggcgccacgt ttgataagtt cttttgatat atcaatggaa
     4321 ggcgcatcgc gtaaatcgtc agtgttgggt ttaaagctga aacctaaaag tgttacccgc
     4381 cgtccgttaa ggtcgccgat cttttcctgt aaggcgttca cgatccactg gcgcaaactg
     4441 tagttgacgt cccgcgcggc ttgcacaatg cgcatttcca gtccattttt cctggccgta
     4501 gcgaccagcg cagcggtgtc tttgccaaaa cacgagccac cccagccaat tccggcgttg
     4561 agaaaacggc tgccgatgcg cgggtcaagc cccatgcctt tggcgagggc atccacatcc
     4621 gcgccaactt tttgcgcgag ataacccatt tcattgatga aagaaatttt tgtagccaaa
     4681 aacgcgttgg cagcatattt tatcgtttca gcggagatta catcgcacac aaaaatgggc
     4741 acttcttgca agccctctgg tcgtggcagg catttgggtg atacaaaatt ttgtttgtgg
     4801 attggctcaa acagcttgct gaaacgctcg atgctgggcg cgtgctgtgt gccgatgatg
     4861 atgcggtttg gatagaatga atcgcgtaat gccgaaccct gcgagagaaa ttccggactt
     4921 gaaataacgt tgaagtctcg cttgccaggt ctgtcatcta aagcgtcggt aaagtcgtga
     4981 atgcggtctt cgagccattt tcccgtgcca attggcacgg tggatttgtt gacgatgagc
     5041 gtaaagtctt cacccagatt ttgggcgagt gactcagcgg cctgggtcag ataggtcaaa
     5101 tcagcgtccc cgtccggcaa tgatggcgtg cctacggcga tgataatgac ttcggtgttg
     5161 ggaatatcgg cttgggcata atcggtggta aagcgcatct tgtgacgcac ctggtcaaaa
     5221 acttcatcaa gatgcggttc atggatgggc aggatacctt gtttgagtct attgattttt
     5281 tcagcattgg tatccaggca ggtgactgaa tgccctatat aagcaagcac tgtggcagtt
     5341 gtaaggccga cataaccagt tccgataacg actacgcgca tattttcatc ctttcaggc
//
LOCUS       DAOSFC010000074         5347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13629_length_5347_cov_4.235828, whole genome shotgun sequence.
ACCESSION   DAOSFC010000074 DAOSFC010000000
VERSION     DAOSFC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13629_length_5347_cov_4.235828"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PK791_02945"
     CDS             <1..1150
                     /locus_tag="PK791_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92302.1"
                     /translation="PWGDFANPDENSTDKPTYYVDVLSSQWLGEGVSTCSTIWQAFLP
                     VDGPPAWLRTPALAEELESTREYFLLAGLLIKQGLVDASACSFGGLEANGAATPCGMD
                     LARPYVQEWQNQFNAEIIRVANQTGVPAQMMKNIFSRESQFWPGIYDRVYEAGLGHLS
                     EQGADTVLLWNPSFFSQFCPLILSEESCQRGFGNLDPDQQALLRGALVQQVNAQCPDC
                     PTGIDLSQANFSISVFARSLLANCEQVGQIVYNSTGRQAGQVATYEDLWKFTLVNYHA
                     GPGCLGNAMQRAIGNDQRLTWENVYPYLEEGCRTGFDYVQDIAAMPDADETGIGDLLS
                     TAEPVPYLPTPTYPPTPWPTRTPTRIPATSTPGPSPTPQPSSTPPIYP"
     gene            1265..1870
                     /locus_tag="PK791_02950"
     CDS             1265..1870
                     /locus_tag="PK791_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92303.1"
                     /translation="MGIYTLAKKTIDENDGVARNVDFQKHGLKNFQITYLCNKGYLER
                     IRRGYYQLGTFDDTTEEKIIARLFPDGILCMDSALFYYGYSDRTPLEWTIAFQRTVTR
                     TRLKVNSPKIKPYFISEETFLLGKTEQTINDVQLAMYDRDRTICDCFKYQNKMDGELF
                     NKAVLAYSKDEKKNIANLSDYAKKMRVYKKMAEILGVLLNG"
     gene            1863..2765
                     /locus_tag="PK791_02955"
     CDS             1863..2765
                     /locus_tag="PK791_02955"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOH92304.1"
                     /translation="MADRAASVLTRLKNQANLTGRTFQLCLQLFCQEEFLRRLAGSKY
                     KNNLVLKGGHLIYCLTEFSSRPTVDIDFLARDTPNTIEALRDVLSTIIDTPTGNDFIL
                     YEIKHIEIISIDKKYPGLSAVLQARIKNTKTPIKIDFGFGDLVYPEDQMRTVPTQLND
                     FIAPLVKTYSLETVVAEKLDAILNLMEFSSRMKDYYDLYFLSVGFDLDGNQLRTALIK
                     IFKNRERTYKQEDMKRVLAFDQNKDMVRKWDTFLKKVKLSPIQFNVVIAGIAAFLEPV
                     WDSIYTNRSFAYSWRAKDGQWLGD"
     gene            complement(2871..3083)
                     /locus_tag="PK791_02960"
     CDS             complement(2871..3083)
                     /locus_tag="PK791_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOH92305.1"
                     /translation="MKKTCSLFTGLFVGALVSSALVLLLTPWSGEELQENIKDFANNF
                     QEEVRQAAAEKRQELEQELAQLRSGK"
     gene            complement(3171..3608)
                     /gene="dtd"
                     /locus_tag="PK791_02965"
     CDS             complement(3171..3608)
                     /gene="dtd"
                     /locus_tag="PK791_02965"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOH92306.1"
                     /translation="MRAILQRVTKGSVTVDGQVVADIGLGYVVLLGVGHEDTQAKARE
                     LAEKIVYLRVFGDDEGKMNRSLLDVAGEMIVVSQFTLHADTSRGRRPSFINAARPELA
                     EPLVEYFIEQLRNFGIKVQSGIFGAHMVVDIQNDGPVTIALEN"
     gene            3784..4923
                     /locus_tag="PK791_02970"
     CDS             3784..4923
                     /locus_tag="PK791_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOH92307.1"
                     /translation="MPLASMPSAELITSPERYQMMIAHLGGQKAIAIDTESNSLFAYQ
                     ERVCLIQLSSRERDYLLDPFAFDDLHALGELMADEHVQKIMHAGDYDISTLKRDYDFT
                     FNNVFDTMLAASALGESSLGLSTLLEKYFGIELAKKYQRANWGERPVKDDMLRYAQAD
                     SHFLIPLRDYLLPRLEAIGWLELVQEDSNAMAKNTPAMKPHCEDVWRIKGTRDLSPAE
                     MSLLAELNHQRELQAQKRDRPPFKIFGDPSMVAIAQAMPQTLEELSALKVFSKHQMKR
                     YGNLIVNTVTNWLEKPTKLHRPAVLRPCDAELERHEALKKLRKTIAEKEGIASNLVLP
                     RDLLEKIARHDPQSMPELESLMSDYPLRFARFGKAIFDLLLRRRL"
     gene            4920..>5347
                     /locus_tag="PK791_02975"
     CDS             4920..>5347
                     /locus_tag="PK791_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH92308.1"
                     /translation="MKLTVFGAARTVTGSMTLIEYGGKKLLLECGTFQGRREESYFTN
                     LNFPFNPAEIDAMILSHAHIDHSGNIPNLVKQGFKGKIYATNATVDLADIMLRDSGHI
                     QENDIKYLNKKRRKKNKPPLEPIYTQKDALAAMPHFRMVW"
BASE COUNT         1455 a         1284 c         1242 g         1366 t
ORIGIN      
        1 accatggggc gactttgcca acccagacga gaactccacc gacaaaccaa cctattatgt
       61 agacgttctt agcagccagt ggttaggcga gggtgtttcg acctgttcaa cgatttggca
      121 ggcttttttg ccggttgatg ggccacctgc ctggttgcgc acgcccgctt tggcggaaga
      181 actcgaaagc acccgtgaat acttcctttt ggcgggattg ctaatcaaac aaggactggt
      241 cgatgccagc gcctgttcct ttggcgggct tgaagcaaat ggcgccgcca cgccatgcgg
      301 aatggacctg gcgcgtccct atgtgcagga atggcaaaat cagttcaacg cggaaattat
      361 tcgcgtagcc aaccaaacgg gtgtgcccgc gcaaatgatg aagaacatct ttagtcgcga
      421 aagtcagttt tggcccggca tctacgatcg cgtttatgag gcgggcttgg gacatttgag
      481 cgaacagggc gcggatacgg ttttgctttg gaaccccagc tttttcagcc agttttgccc
      541 gcttatcctt agcgaagaaa gctgtcagcg tggttttggc aacctggacc cggaccagca
      601 agcccttttg cggggcgcgt tggtgcaaca ggtaaacgcg caatgccccg attgtcccac
      661 aggcattgac cttagccagg cgaattttag catcagtgtt ttcgcgcgca gccttttggc
      721 gaattgcgaa caagtggggc aaattgttta caacagcacg ggacgccagg ctggccaggt
      781 ggcgacttat gaggatttat ggaagtttac tttggtgaac tatcatgccg ggccgggttg
      841 tttgggtaac gccatgcaac gcgcgatagg caatgaccag cgcctgacct gggagaatgt
      901 ttacccttat cttgaagaag gttgtcgaac gggctttgat tacgtgcagg atatcgcggc
      961 gatgcctgat gcagatgaaa ctggtatcgg tgatttgctt agcacggcgg aaccggttcc
     1021 ctatctgccc acgcccacct atccgccaac gccctggccc acgcgcacgc caacgcgcat
     1081 acctgccaca tccacgccgg gcccatcgcc aacgccgcaa ccgagttcaa caccgccaat
     1141 ttatccttag tctattttaa agacccgata ataatgcgaa agtactaatt gatttatttt
     1201 gtaaaaaagc gtatactcta accatacaca aacatgagtg cttgtgtatg gttaggagac
     1261 tgaaatgggt atttatacac ttgcaaaaaa aacgattgat gaaaatgatg gcgttgccag
     1321 aaatgtagac tttcaaaaac acggtctaaa aaatttccag attacatatc tttgcaataa
     1381 aggatatctt gaacggatca ggagaggcta ctatcagctt ggtacatttg atgatacgac
     1441 agaagaaaaa atcatcgcca ggcttttccc agatggaatc ctatgtatgg attccgctct
     1501 tttctattac ggttatagcg atcgaacgcc actggaatgg acaattgctt ttcaacggac
     1561 tgtcacaaga acccgcttga aagttaattc tccaaaaata aaaccctatt ttatctctga
     1621 agaaactttt ctgcttggta aaactgaaca gacaatcaat gatgtccagc tagctatgta
     1681 tgacagagac cgaactattt gtgattgctt caaatatcaa aacaagatgg atggcgaact
     1741 ttttaataag gcagttttgg cgtatagcaa agatgaaaag aaaaacatcg ccaatctttc
     1801 tgattacgca aaaaaaatgc gggtatataa aaaaatggct gaaatcctgg gagtattgct
     1861 caatggctga tagggctgct tctgttttaa caagattgaa aaaccaagct aatctcacag
     1921 gtagaacctt ccagttgtgt ttgcagttat tctgtcagga agaattcctg cgtcgattag
     1981 caggctcaaa atacaaaaat aatttagttc taaaaggtgg acacctgatt tactgtctaa
     2041 ctgaattcag tagccggcca acagtagata tcgattttct ggctcgagat acaccgaaca
     2101 ccattgaagc tttgagagat gtcctttcga ctattatcga caccccaact gggaacgact
     2161 ttatccttta cgaaattaag catattgaga ttatttcgat agacaagaag tatccaggct
     2221 tgtccgcggt actacaggca cgtataaaga atacaaaaac accaataaaa atcgattttg
     2281 gttttgggga tctggtttat cctgaagatc agatgagaac agtcccgaca caattaaatg
     2341 attttattgc tccgctggtt aagacctatt ctcttgagac agtagttgct gaaaaactgg
     2401 acgcaattct caacttgatg gaattttcaa gtcgaatgaa agattactat gacctgtatt
     2461 ttctttctgt aggatttgac cttgacggaa accaattgcg tacagcctta ataaaaattt
     2521 ttaaaaacag ggaacgaaca tataaacagg aagacatgaa aagagttctt gcttttgacc
     2581 agaataaaga tatggtaagg aaatgggaca cttttttgaa aaaggtaaaa ttgtccccga
     2641 tccagtttaa tgtggtaatt gctggcattg ctgcattcct tgaaccagta tgggattcca
     2701 tttatacaaa tcgttctttt gcttattctt ggcgtgcgaa ggatggccaa tggcttggcg
     2761 attgaatctt ctgtttgaag atttcacttc agttttcttt ctgccacaaa ttgcttaaac
     2821 aaaaaccgcc atctaagagt ggcggtttta cttgactaaa tagttgacga ttatttgcct
     2881 gaacgtaact gggcgagttc ctgttccagt tcctgacgtt tttccgcggc agcctggcga
     2941 acttcttcct ggaaattatt ggcaaaatct ttgatgtttt cttgcagctc ttcacccgac
     3001 cagggtgtga gcaacagaac cagcgcactg ctaacaagcg cgccaacaaa cagacctgta
     3061 aaaagtgagc atgttttttt cattttagtt cctttctcca ttcatcttta ttataaagga
     3121 cttacgcctg atatgaacag aattaattaa gattttacac tctccaaacc ttaattttcc
     3181 aaagcgatgg ttacaggccc atcgttttgg atatccacca ccatgtgcgc gccaaaaatg
     3241 ccactttgca ccttgatgcc aaagttgcgc agctgttcga tgaaatactc aaccagcggt
     3301 tcggcgagtt cgggcctggc ggcgttgata aaactcggac ggcgtccgcg gctggtatcg
     3361 gcgtgcaaag tgaactgcga aacgacaatc atctcgccag ccacatccag gagagagcgg
     3421 ttcattttgc cttcgtcgtc gccaaacacg cgtaaataaa cgattttctc agcaagttcg
     3481 cgcgctttgg cctgggtgtc ttcatgccca acgcccagca agacgacgta tcccaaaccg
     3541 atatcagcca caacctggcc gtcaaccgtc acactgcctt ttgtcacccg ttgtaaaatc
     3601 gcgcgcatcg ttctctccaa aatcctgtca ttgttttatt ttgtggcttg tggtcgggag
     3661 ttcacaagcg aacaaatggt tttcctggtt cggatatggt ctcctgacca tgtccatcac
     3721 aagatgagcc ttgccaagtc tgattatagt cggctttggt caatcggggt gtataattga
     3781 gacatgcctt tagcttcaat gccatccgcc gagctgatta caagccctga gcgctatcaa
     3841 atgatgatcg cgcacttggg tggacaaaag gccatcgcta tcgacaccga atccaacagc
     3901 ttatttgcct atcaagagcg cgtttgtctt atccagcttt catcgcggga gcgtgactat
     3961 ctgcttgacc ccttcgcctt tgacgatttg cacgccctgg gcgagctgat ggcggatgag
     4021 cacgttcaaa aaatcatgca tgccggtgat tatgacatct caacgctaaa acgcgattat
     4081 gatttcacgt tcaacaatgt ttttgacacc atgctggccg cgtcggcttt gggggagagc
     4141 agtttgggct tgagcacact gcttgaaaaa tacttcggca ttgaactggc caaaaaatac
     4201 cagcgcgcca actggggcga acggcctgtc aaggatgata tgttgcgcta cgcccaggct
     4261 gactcgcatt tccttatccc tttgcgcgat tatttgctgc ccaggctgga agcaatcggc
     4321 tggcttgaac tcgtgcagga agattcaaac gctatggcca agaacacgcc cgcgatgaaa
     4381 ccccactgcg aagacgtctg gcggataaaa ggcacgcgcg atttgagccc cgcggagatg
     4441 agcctgctgg ctgaactcaa ccatcagcgc gaactgcagg cgcaaaaacg cgaccgccca
     4501 ccgtttaaaa tctttggcga cccatcgatg gtggccatcg cccaggctat gccccaaacg
     4561 ctcgaagagc tttccgcgct caaggtcttt tcgaaacacc aaatgaagcg atatggcaat
     4621 ctgatcgtaa acaccgtgac caactggctc gaaaagccca ccaaactgca tcggccagct
     4681 gttttgcgtc cctgcgacgc tgaactggaa cgccacgaag ccttaaagaa gttacgcaaa
     4741 accatcgcgg aaaaagaagg cattgccagc aacctggtac tgccccgcga tttactggag
     4801 aaaattgccc ggcatgaccc ccaaagcatg ccagagctgg aaagtttgat gagcgactac
     4861 ccactgcgct tcgcgcgttt tggcaaagcg atatttgatt tattactaag gagaagacta
     4921 tgaaattaac cgtttttggt gcagcccgta cggtaaccgg atcaatgacg ctgattgaat
     4981 acggtggcaa aaaacttttg ttggaatgtg gcacttttca gggccggcgc gaagagtcct
     5041 acttcacgaa tctgaacttc ccctttaatc ccgcggaaat tgacgcgatg attctctcgc
     5101 atgcccacat cgaccatagt ggcaacattc ccaacctggt caagcaaggt ttcaaaggta
     5161 aaatttacgc caccaacgcc acggtcgatt tggcggatat catgttgcgc gactcgggac
     5221 acatccagga aaatgatatc aaatatctta ataaaaagcg ccgaaaaaag aataaaccgc
     5281 ctttggagcc catctacacc caaaaagatg ccctggcggc catgccccat tttcgcatgg
     5341 tttggta
//
LOCUS       DAOSFC010000075         5321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13727_length_5321_cov_4.355488, whole genome shotgun sequence.
ACCESSION   DAOSFC010000075 DAOSFC010000000
VERSION     DAOSFC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13727_length_5321_cov_4.355488"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..866
                     /locus_tag="PK791_02980"
     CDS             156..866
                     /locus_tag="PK791_02980"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOH92309.1"
                     /translation="MENNETIQRMPMIGDPAPEFKAKTTQGDINFPSDYAGHWVILFS
                     HPADFTPVCTSEFISFAVLEEEFAKLNTKLVGLSVDGLMSHIAWLRTIKEKIEFNGHK
                     NVEVKFPLIDDLTMEVAKKYGMIMPNASSTQAVRAVFVIDPKGIVRAIIYYPLSTGRN
                     FDELIRLVQALQTADEFGVATPADWRPGDKVIVPPPGSCGVATQRMAEQKDMECQDWF
                     FCLRPIDKEEVFSKIHKS"
     gene            complement(979..2175)
                     /locus_tag="PK791_02985"
     CDS             complement(979..2175)
                     /locus_tag="PK791_02985"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOH92310.1"
                     /translation="MIKRMLKFALIVLLFALISTVPGALAVDAADGQAHYDLPLCLPG
                     HYQTDPGDCLPLGPSQSIREMAEQGFPYPMRGLSARRPDASLSELPEPVALVTGGNTP
                     FYASLDDAMAGATPVRTIVGEGGRYVSVTESVQAGGRTFVRTKKGEWVEATPIWGWPR
                     WQGLEFFATPNHNFGFTIDDIYSYSEPSFISNQTENFYKKYDSFPIYSWEEAEGFTWY
                     HVAPGEWIPSLKSRMVRVDTHRPDGVDRDRWIAIDLEQQTLAVYEDGELRFATVIASG
                     AGQAYSDPGVYEVYHKVELHTMQGSYAMDKSDFFYYENVPWALYYNHAQAIHAVYWPA
                     VLGIPQSHGCINMFPGDANWLFHWAELGEYVYVFDPSGKTPLPTPTPMGTPPQPIYTP
                     TPSSGG"
     gene            2539..>5321
                     /locus_tag="PK791_02990"
     CDS             2539..>5321
                     /locus_tag="PK791_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOH92311.1"
                     /translation="MQTIVCLDIETTGLSPDNDSIIEIGAIKFSGTRVIAEYETLINP
                     SRPINQYISNLTGISNAMVMNAPMLSDKLREFVDFVGDAPILGQNVGFDLSFFHKVGA
                     LKQNQAIDTYELASVLLPTAPRYALSALASHLGVVQQNVHRALADARATMDVYHKLMD
                     ILHTLPDELIVEIVSQSEKLGWAGELPFKAVLKERMEDRPYEIYKAQKATDKLFSFAK
                     AIPAIPLEPVQFLKPLDVEELASTLEPGGAFSRHNLDFEHRSQQVQVLKSVAEALNKG
                     NHLMVEAGTGTGKSLAYLIPAAQWALQNGERVVVSTNTSALQDQLINKDLPDLIEALD
                     SDLRTAVLKGRSNYLCPRKLNALRKRGPENADELRILAKILVWLHQGGSGDRAEINLN
                     GPIERMIWARLSAEDENCRIETCLRQMGGRCPFYKARMAAESAHILVVNHALLLADAA
                     TENRVLPAYNYLIIDEAHHIEAATTDAMAFRLYAHDIKRLVRELGSHEHGLLGRAWAL
                     AQDGLRPSDLAQLSVTIGKITDIAVRFDTYMSQYFKALDYVLEELRDGRPLGTYPQQV
                     RILPSLRSLPMWFEVEVAWDDAHTALDSLTSHLKVIREILHSINSNDDEDAEDLMGAL
                     GTMSQTIAEIGEKLEQLTMTPDDNQIYWVELDPLQGRLTLQSAPLHIGTLMEKHLWHE
                     KASVILTSATLTTHGEFDYLKRRLNAQDADEMTIDSPFDYEHAVMIYLPTDIPEPNDS
                     YGHQKITEDTIIRLARVTGGRMLVLFTSYAQLRKTSQAISPALAKLDISVFEQGEGAS
                     ASALLESFKESDRAVLLGTRAFWEGVDVPGEALSALVIVKLPFDVPSDPIIAARAESF
                     ENPFAEYNLPEAILRFRQGFGRLIRTQSDRGVVVMLDKRLVSKQYGRLFMESLPNCHI
                     QRSSIANLPEHAS"
BASE COUNT         1378 a         1605 c         1171 g         1167 t
ORIGIN      
        1 ctgcttgatg acatgaaagc ctatgttatt gaacaaagcg actacgatat catcaacagt
       61 cgccacattt actacggcat ttgccctaac tgtaaatcga cttcagtcaa ctctgaagat
      121 aaaaatcaaa attaaaccat acaaggagat taactatgga aaacaacgaa acaatccaac
      181 gcatgcccat gatcggtgat cccgcacccg aatttaaagc caaaacaacc cagggcgaca
      241 tcaacttccc atcagattac gccggacact gggttattct cttcagccat cccgctgact
      301 ttacccctgt ttgtaccagc gaatttatca gctttgccgt tctcgaagaa gaatttgcca
      361 agctcaacac caaactggtc ggcctctcgg ttgacgggct gatgagtcac atcgcctggc
      421 tgcgcaccat caaagaaaaa atcgaattca acggtcacaa gaacgtggaa gtcaaattcc
      481 ccttaattga tgacctcacc atggaagttg ccaaaaaata cggcatgatt atgcccaacg
      541 cttcttccac acaggctgta cgcgccgtct ttgtgatcga ccccaaaggc atcgttcgcg
      601 ccatcatcta ctacccactt tcaaccggac gcaactttga cgagctcatc cgcttggttc
      661 aggctctgca aaccgccgat gaatttggcg tagcaactcc tgccgactgg cgcccaggcg
      721 ataaggtcat cgtgccaccc cccggatcct gtggtgtcgc cacccagcgc atggctgagc
      781 aaaaggatat ggaatgccag gactggttct tctgtcttcg cccgattgat aaggaagaag
      841 tctttagcaa gattcacaaa agctaatctt gattagctgg taaagtttag tttacttgcc
      901 aaagcattga aataaattaa aactgaccgt tcaatcatag agcggtcagt tttgttttta
      961 tgacaataac ccagatcttt agccgccaga actgggcgtt ggcgtgtaaa tcggctgagg
     1021 tggcgtgccc atcggcgttg gggttggcaa gggtgtcttt ccgcttgggt caaagacata
     1081 cacatactca cccaactccg cccagtggaa taaccagttg gcatcgcccg ggaacatatt
     1141 gatacagcca tgcgactggg gaatgcccaa aactgccggc caatacactg catgaattgc
     1201 ctgcgcgtga ttgtaataaa gcgcccaggg aacattttca taatagaaga aatctgactt
     1261 atccatggca tacgagccct gcatggtgtg taattcaacc ttatgataga cctcatacac
     1321 acctgggtca gaataagcct ggcccgcgcc actggcaatt accgtagcaa agcgcaattc
     1381 accgtcttcg tagaccgcca aagtctgctg ttccaggtca atcgcaatcc acctgtcgcg
     1441 gtcaacacca tcaggccgat gggtatctac ccgcaccatg cgcgatttta gcgatggtat
     1501 ccactcaccc ggtgcaacat gataccaggt aaaaccctca gcttcttccc aactgtatat
     1561 cggaaacgag tcgtactttt tataaaagtt ttccgtttgg tttgaaataa aactcggttc
     1621 agaataactg taaatgtcat caatcgtaaa gccaaaattg tgattagggg tagcaaagaa
     1681 ttccagtccc tgccagcgtg gccagcccca aatcggtgtt gcctcaaccc attccccttt
     1741 tttggtacgc acaaaggtac gcccgccagc ttgcaccgat tcagtcacac tcacataacg
     1801 cccgccttcg cccacaatcg ttcgcaccgg cgtggcacct gccatggcat catccaaact
     1861 ggcatagaag ggcgtattgc caccagtcac cagcgccaca ggttccggca attccgaaag
     1921 gctggcatcc ggccttcgcg cggaaagccc ccgcatcgga tacggaaagc cctgttcagc
     1981 catctcacga attgattgcg atggacccaa gggcaagcag tcccctgggt cagtttgata
     2041 atggccaggc aaacacaaag gcaggtcata atgggcttgc ccgtcagcag cgtccactgc
     2101 caacgcgcct ggtacagtgc ttatcagggc aaacaacaag actatcaaag cgaattttag
     2161 cattcgtttt atcatgctcc ctattatacc ttgcccaaat gaggatgcaa aaaggctgtg
     2221 tcagccatgc catgacaaac acaacttatc ggacgttgtt ttctatagca atcacaacct
     2281 gactaaacaa aaaattcccc ctctccaggg agagggataa agggtgtggg tttttaaagt
     2341 attacaaaca tttttattgt gggttatcca aacctctgct tcactttttt gatgatattc
     2401 gctaaatata attcgcggtt cttgaattca acccctttta atttcctggt tccaaaaaac
     2461 catttttctc accgattacc ctgcattatc cctgcccaca agccaccagg gacaggcaaa
     2521 gaaggtataa tcaaacccat gcaaaccatc gtctgccttg acatagaaac aaccgggcta
     2581 agccccgaca acgactccat catcgaaatc ggcgccatca aattttccgg cacccgcgtt
     2641 atcgccgaat acgaaacctt aatcaacccc agccgcccga ttaatcaata catcagcaat
     2701 ctcaccggca tttccaacgc catggtcatg aacgcgccca tgctctcaga caaattgcgc
     2761 gaatttgtcg acttcgtcgg tgatgcgccc attcttggtc aaaacgtggg ttttgatctc
     2821 agtttcttcc acaaagttgg cgcgctaaag caaaaccaag ctatcgacac ctacgaactg
     2881 gcctccgttc tgcttcccac cgcgccccgc tacgccctaa gcgccctggc aagtcacctg
     2941 ggggttgtac aacaaaacgt ccaccgcgcg cttgcggatg cccgcgccac catggacgtc
     3001 tatcacaagc tgatggatat cctccacact ttgcccgacg aactcatcgt tgaaattgtc
     3061 agtcaatccg aaaaactggg ctgggcgggc gaactgccct tcaaagccgt gctcaaagaa
     3121 cgcatggaag accgccctta cgagatttac aaagcccaaa aagcaaccga taaacttttc
     3181 agtttcgcaa aagccatacc cgccatcccc ttggaaccgg ttcaattcct caaaccgctt
     3241 gatgttgaag aactcgccag caccctggaa cccggaggcg cgttttcgcg tcataacctc
     3301 gactttgaac accgctcaca gcaagtgcag gtgctcaaat cagttgccga agcgcttaac
     3361 aaaggcaatc atctcatggt cgaagccggc accggcaccg gcaaatcgct ggcatatctc
     3421 attcccgccg cgcagtgggc cttgcaaaac ggcgaaaggg tcgtcgtttc taccaacact
     3481 agcgccttgc aagaccagct catcaacaaa gacctgcccg acctcattga agccctggat
     3541 tcagacctgc gtaccgctgt tctcaaagga cgctcaaatt acctctgccc gcgcaaactc
     3601 aacgcattgc gcaaacgcgg ccccgaaaat gctgacgaac tgcgcatcct ggccaaaata
     3661 ttagtctggc tccatcaggg tggcagtggc gaccgcgctg aaatcaacct gaacggcccc
     3721 atcgaacgca tgatttgggc gcgtctctcc gcggaagacg aaaactgtcg catcgaaacc
     3781 tgtctcaggc aaatgggcgg acgctgtccg ttctacaaag cgcgcatggc agccgaaagc
     3841 gcgcatattc tcgtcgtcaa ccacgccttg ctgcttgccg atgccgccac cgaaaaccgc
     3901 gttttaccgg cgtataacta cctcattatt gacgaagccc accacatcga agccgccacc
     3961 accgacgcca tggctttccg cctttacgcg cacgacatca aacgccttgt ccgcgaactg
     4021 ggcagccacg aacacggcct gctgggtcgt gcctgggcgc tcgctcagga tgggcttaga
     4081 ccttccgacc tcgctcagtt gagtgtcacc atcggaaaaa tcaccgatat tgctgtgcgc
     4141 ttcgacacct acatgagtca atacttcaaa gcgctggatt acgtgcttga agaactgcgc
     4201 gacggtcgcc cactgggcac ctacccccaa caagtacgca ttttgccctc cctgcgctcc
     4261 ctgccgatgt ggttcgaagt tgaagtcgcc tgggatgacg cccacaccgc gctcgacagt
     4321 ctcacttcac atctcaaagt catccgcgaa atcctccaca gcattaacag caacgatgat
     4381 gaagatgccg aagacctcat gggcgctttg ggcacgatga gtcaaaccat cgctgaaatc
     4441 ggcgaaaagc tcgaacaact caccatgacc cccgacgaca atcaaatcta ctgggttgaa
     4501 ctcgacccct tacaagggcg cttaaccctg caaagcgcgc ctttgcatat cggcaccctt
     4561 atggaaaaac atctctggca cgaaaaagcc agcgtcattc tcacttccgc caccctgacc
     4621 acccacggtg aatttgacta tctcaaacgt cgcctcaatg cccaggacgc cgacgaaatg
     4681 accatcgact cgcctttcga ttacgaacac gctgtaatga tttacttacc caccgacatc
     4741 cccgaaccca acgacagtta cgggcatcaa aaaatcacgg aagataccat catccgcctg
     4801 gcgcgcgtca ccggtgggcg tatgctggtg cttttcacct cttacgcgca actccgcaag
     4861 acctcccagg ccatcagccc cgccctggct aaactggata tttccgtttt tgaacaaggc
     4921 gaaggcgctt cggcatcagc cctgttggaa tctttcaagg aaagcgaccg cgccgtcctg
     4981 ctgggcaccc gcgccttttg ggaaggggtc gacgtccctg gcgaagcgct ctccgcgctg
     5041 gtcatcgtca agctgccttt tgacgtgccc tctgacccca tcatcgccgc gcgcgcggaa
     5101 agttttgaaa atccctttgc cgagtacaac ctgcccgaag ccattttacg tttccggcaa
     5161 ggctttggcc ggcttatccg cacccaaagc gaccgcggcg tggtggttat gctggataaa
     5221 cgcctggtta gcaagcaata cgggcgttta tttatggaat ccctgcccaa ctgccacatc
     5281 cagcgctcat ccatcgccaa ccttcccgaa cacgccagca a
//
LOCUS       DAOSFC010000076         5312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13757_length_5312_cov_5.006468, whole genome shotgun sequence.
ACCESSION   DAOSFC010000076 DAOSFC010000000
VERSION     DAOSFC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13757_length_5312_cov_5.006468"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3222
                     /gene="rpoC"
                     /locus_tag="PK791_02995"
     CDS             <1..3222
                     /gene="rpoC"
                     /locus_tag="PK791_02995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOH92312.1"
                     /translation="TVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPEVIVRNEKRMLQEAVDSLIDNSQRGRMLSRRGRRELRSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPHLKLYQCGLPKSMALELYRPFVIAGLDRHGYTTNIKGAKR
                     FIERNRPEVWEVLEEVIQDRPVLLNRAPTLHRLGIQAFEPVLIEGSAIQLHPLVTTAF
                     NADFDGDQMPVHVPLSDKAVAEARELMLATKNLLKPASGEPIISPGKDMVLGVYYLTM
                     IDDAPQKGDGRVFGDIDEVILAYQLGQVHVHTKIRLRARTWFDDDHNRMSKPEIRIID
                     TTVGRAMFNDILPDKAQFTNMPLDKGGIRDLIEDIYEIDGQEETGVVADAIKDIGFRY
                     ATQSGTTLAIADITVPPEKPALIKEALLEVEQVQKEYKRGLLTEQEQNERIIEIWQDT
                     SKRVSDAVRNHMDPVGNLSTMAISGATKGGFGPIAQMAGMRGLMADPSGRIIPMPIQS
                     SFREGLDALEYFISIHGQRKGLADTALRTAEAGYLTRRLVDVAQDIIINSDDCGTHEG
                     ITLYADDKSAGLPFRDRLRGRVSANRVIHPETGEVIAQPNDFITKDLARRIVAANVKE
                     VKVRSALTCELVHGVCANCYGEDLGRGRMVELGAAVGIVAAQSIGEPGTQLTLRTFHT
                     GGVAAGGDITTGLPRVEELFEARRTPKGEAIITEIAGTAYIKQSEKHLDQRSVQVDFS
                     EMVPEPYEILEGWTIKVADGQEIASGDVIAELDEATINATHGGKVRIEGNTVIVSYEN
                     KLSETYEIPTNSRIIITDGQKVEAGQQLTEGSLNPHTILKIKGREETERYLLREIQQV
                     YRTQGQNINDKHFEVIIRKMLNKVQITRPGDTDFLPQDLVDRLEILKLNEDLLAKGMK
                     PARYNEVLLGISKASLSTDSFLSAASFQHTIKVLAGAAISSKVDPLYGLKENVIIGKL
                     IPAGTGFRPGRFDIDKQDVEEDADADTHEQLAMFGDDEDIDELFDDVDYDDEDTDEDY
                     DEDLDIDEDEELDVDIDSDEDFDEELTFDEDDETYSEEDDDEN"
     gene            3338..>5312
                     /locus_tag="PK791_03000"
     CDS             3338..>5312
                     /locus_tag="PK791_03000"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HOH92313.1"
                     /translation="MSKSDVKIVNINHEMQQSYLDYAMSVIVSRALPDARDGLKPVQR
                     RILYAMYDMGIRAETPHKKSARIVGEVLGKYHPHSDSAVYDAMARMAQDFVMRYRFVD
                     GQGNFGSIDGDPPAAMRYTEARLSAEAIEMLSQLDMDTVDYNDNFDGTLKEPSVLPAA
                     IPAMLVNGASGIAVGMATNIPPHNLGEIVDALVMMLQNWETLDDITIEEIMQYVKGPD
                     FPTGGLILQDETSDSLAQIYGSGNGIVQMRARVRLEELSRGRVRIIVSELPYNVNKST
                     LIERIAEISRDGGLEGISDLRDESDRQGMRIVIDLSKNADPEKIMATLYKRTQMHASF
                     GVNILALVDGSPHRLPMKQALRVFLDHRLEVVRRRSEFLLKKNRDRLHILAALRIAIQ
                     NIDEVIRIIRKSKTVDDARQSLMTKFKLDEIQANAILEMPLRRLASLERQKIEDEYKS
                     VSKTIKDLEALLKSPKAMRNVIITELKEIREKYADPRRTQIIRFGEGEKASEMFKHTD
                     VMPLENFWIELKENGLISRTGSDKQSRLGGNDAPWQMLRTNSHHVVYLVTTTGKAAAI
                     SSVAVPSQLQFEDGQHTAQISPLDESDLVQSVITVPMERDALPDRFVVSVTRSGMIKR
                     SEVAELPGASASSFVLARTNEGDEILTVLFTKGG"
BASE COUNT         1472 a         1100 c         1354 g         1386 t
ORIGIN      
        1 actgttttac cggttattcc tccagattta cgtcccatgg tgcaattgga tggtggccgt
       61 tttgctacat ctgacctgaa cgatctctac cgtcgtgtga ttaaccgtaa taatcgctta
      121 aaacgcttgc ttgaacttgg cgcacctgaa gttattgtgc gaaatgagaa gcgcatgctt
      181 caggaagcag tcgactcatt gattgacaac tcacaacgtg gtcgcatgtt gtcacgtcgc
      241 ggaagaaggg aattacgctc tttgagcgat atgctcaagg gtaaaaaagg acgcttccgc
      301 cgcaaccttt tgggtaaacg tgtggactac tctggccgtt cagtaattgt ggttggtccg
      361 catttgaagc tttatcaatg cggtttgcct aaatcgatgg ctttggagct ctatcgaccc
      421 tttgttatcg cgggtttgga tcgtcatggt tacactacaa acattaaggg cgctaaacgt
      481 tttatagaac gcaaccgtcc tgaagtttgg gaagtgcttg aagaagttat tcaggaccga
      541 cctgtactgc tcaaccgcgc gcctacgctt caccgtttgg gtattcaggc ttttgaaccc
      601 gtgctaattg aaggcagtgc gattcagttg catccattgg tgacaactgc tttcaacgcg
      661 gactttgacg gtgaccagat gcctgtgcat gtgccactgt cggacaaagc agttgctgaa
      721 gcccgcgaat tgatgttagc aaccaaaaac ctgctcaagc ctgccagtgg tgagccgatc
      781 atcagtcctg gaaaggatat ggtattaggt gtgtattatc tgaccatgat agatgacgcc
      841 ccacaaaaag gcgatggacg tgtatttggc gatattgacg aagtcatttt ggcttaccag
      901 cttggccaag tccatgtgca tacaaaaatc agacttcgcg ccaggacctg gtttgatgat
      961 gaccataacc gaatgagtaa acctgaaatt cgcatcattg acacaacggt tgggcgtgct
     1021 atgtttaatg atattttgcc tgataaagct cagtttacaa atatgcccct ggataagggc
     1081 ggtatccgag acttaatcga agatatctat gaaatcgatg gacaggaaga aacaggagtt
     1141 gttgccgatg cgattaagga tattggtttc cgctatgcta cacaaagcgg tacaaccttg
     1201 gcaatcgcag acatcaccgt tccgcctgag aaacctgctt taatcaaaga ggcgttactt
     1261 gaggttgaac aggttcaaaa agaatacaag cgtggtctgc ttaccgagca ggaacaaaac
     1321 gaacgcatta ttgaaatttg gcaggatact tccaagcgtg tttctgacgc tgttcgtaac
     1381 catatggatc ccgttggtaa tctttccacc atggctattt ctggtgctac caagggtggt
     1441 tttggcccta ttgcccaaat ggcgggtatg cgtggtttga tggccgaccc cagtggtcgt
     1501 attattccta tgcctattca atcaagcttc cgcgaaggtt tggacgcatt ggaatacttt
     1561 atttccattc acggtcagcg aaaaggtttg gctgataccg ctctacgtac ggcagaagca
     1621 ggttatttga cccgacgttt ggtggatgtt gcccaggata tcatcattaa cagcgatgat
     1681 tgcggtacgc atgaaggcat tacgctttat gctgatgaca agagcgctgg attgcctttc
     1741 cgtgaccgtc tgcgcggtcg tgtttcggca aaccgcgtta ttcatcctga aacgggtgaa
     1801 gtaatcgccc agccaaacga cttcattacc aaagacctgg cccgtagaat tgtggccgcg
     1861 aatgtaaaag aagttaaagt ccgttccgcc ctgacctgtg aactggtgca tggtgtttgc
     1921 gcgaactgct acggtgaaga tcttggccgt ggcagaatgg ttgagcttgg cgccgcggtt
     1981 ggcatcgttg ccgcacagtc cattggtgaa ccgggtacgc agcttacgct acgaactttc
     2041 cacactggtg gtgttgcagc aggtggcgat attacgactg gtttgccacg tgtggaagaa
     2101 ctttttgaag cacgacgcac accaaagggc gaagcgatta ttactgaaat tgcgggcacc
     2161 gcttacatca aacaaagtga aaaacacctt gaccagcgtt ctgttcaggt cgattttagc
     2221 gaaatggttc ccgaaccgta tgaaattttg gaaggttgga ccattaaagt tgctgacgga
     2281 caggaaattg ccagtggcga cgtgattgct gaactggacg aagcaacgat taacgctact
     2341 catggtggta aagtacgtat tgaaggcaac acagtaatcg tttcctatga gaataaactt
     2401 tcggaaactt acgaaattcc gacaaactca cgtatcatta ttacggacgg tcaaaaagtt
     2461 gaagctggtc aacagttaac tgaaggttcc ttgaacccac atactatctt gaagattaaa
     2521 gggcgcgaag aaacagagcg atacctgttg cgtgaaattc agcaagttta ccgtacgcag
     2581 ggtcaaaaca tcaacgataa gcactttgaa gtgattatcc gaaagatgct taacaaagtt
     2641 caaattaccc gtcctggtga taccgatttc ctgccccagg atttggttga ccgcctggaa
     2701 attctaaaac tcaacgaaga cttgttggct aaaggtatga aacccgcgcg ctacaacgaa
     2761 gtgcttttag gaatttcaaa ggcatctttg agtactgata gtttcctttc ggcagcaagc
     2821 ttccagcaca ccattaaagt tttggctggt gcggcgattt cttcaaaggt agatccgttg
     2881 tatggtctga aagagaacgt tattatcggc aagttgattc ctgctggcac gggctttaga
     2941 ccaggacgct ttgatattga taagcaagac gttgaagaag atgctgatgc agacacccac
     3001 gaacaactgg cgatgtttgg tgatgatgaa gatatcgacg aactctttga tgacgtggat
     3061 tacgatgatg aagacacgga cgaagactac gacgaagatc ttgacattga tgaagatgaa
     3121 gaactggatg tggatatcga ctcggatgaa gattttgatg aggaattgac atttgatgag
     3181 gacgatgaaa cttacagtga agaggatgac gacgaaaatt aatcgtccct aaagcaagaa
     3241 gaaaaacccg gaatgttttc gctccgggtt tttttatgct atatttaatc agcccggaat
     3301 tgcattcaat ccaaaggatt acctaaaagg attaactatg agtaaaagtg atgtgaaaat
     3361 cgttaatatc aaccacgaga tgcaacagag ttatctggat tacgcgatga gtgttatcgt
     3421 ttcgcgtgct ttgccagatg ccagagatgg tttgaaacca gtccaaagac gtattcttta
     3481 cgcgatgtat gacatgggaa ttcgtgcgga aacaccccat aagaaatcgg cgcgtattgt
     3541 tggtgaggtt ttaggtaaat atcatccaca cagtgattca gcggtatacg atgctatggc
     3601 gcgtatggct caggattttg tgatgcgtta ccgctttgtg gatgggcaag gaaactttgg
     3661 cagtattgat ggtgatccac ctgcggcaat gcggtacacc gaagcccggc ttagtgccga
     3721 agccatagaa atgttaagtc agttggacat ggataccgtt gactataacg ataatttcga
     3781 tggaacgcta aaagaaccca gtgttttgcc agcggctatt ccagcaatgt tggtgaatgg
     3841 tgcatcaggt attgctgtgg gtatggcaac gaatatccca ccgcataatt tgggagaaat
     3901 tgttgatgca ttggttatga tgctacagaa ctgggaaacg ctagacgata ttaccataga
     3961 agaaattatg caatatgtca aagggcctga ttttccgacg ggtggtttga tattgcaaga
     4021 tgaaacgagc gattctttag ctcaaatcta cggaagcggg aatggcattg tgcaaatgcg
     4081 tgcaagggtt aggcttgaag aattatcgcg tgggcgggtg cgcattatcg tctcagaatt
     4141 gccttacaac gtgaacaaat caacgcttat tgagcgcatt gccgagatta gccgtgatgg
     4201 gggcttggaa ggaatctcgg atttacgcga cgaatctgac cgccagggca tgcgcattgt
     4261 cattgattta agtaaaaatg cagaccctga aaaaataatg gcaactttgt acaaacgcac
     4321 acagatgcat gcatcgtttg gtgttaatat ccttgctctc gttgatggca gtccgcaccg
     4381 tttgccgatg aaacaagctt tacgcgtttt tctggaccac cgtttggaag ttgtgcgtcg
     4441 tcgaagcgaa ttcttactta agaaaaacag agacagactt catatattag ccgcgctgcg
     4501 catagctata caaaatatag atgaggttat tcgcatcatc cgtaaatcta agactgtgga
     4561 tgatgcacga caaagtttga tgactaaatt caaactggat gagatacagg caaatgccat
     4621 tcttgaaatg ccattgcggc gtctggcatc gcttgagcgt caaaagatcg aggatgaata
     4681 caaatctgta agcaagacta taaaagacct ggaggctctg ctaaaatcgc caaaggcgat
     4741 gcgaaacgtg attatcacgg aattaaagga aatacgtgaa aaatatgcag acccacgccg
     4801 cacacaaatt attcgctttg gggagggcga aaaggcttcg gaaatgttca aacatacaga
     4861 cgtgatgcca ctggaaaatt tttggataga actcaaagaa aatggcctga tttcccgcac
     4921 tggcagcgac aaacaaagca gattgggtgg taacgatgct ccctggcaaa tgttgcgcac
     4981 taactcgcat catgtcgttt acctggttac aaccacaggg aaggcggctg cgatttcttc
     5041 ggtagctgtt ccgtcacagt tacagtttga ggacggacag catactgctc aaatatcacc
     5101 tttggacgaa agcgatctgg ttcaatcggt gataactgtg cctatggaac gcgacgcgct
     5161 tccggaccga tttgttgtta gtgtgacgcg gagcgggatg attaaacgct cagaagttgc
     5221 tgagttgcca ggtgcttcag ccagcagttt tgttttggcg cgaacgaacg aaggtgatga
     5281 gatccttacg gttttattca caaaaggtgg gc
//
LOCUS       DAOSFC010000077         5286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_13855_length_5286_cov_5.671382, whole genome shotgun sequence.
ACCESSION   DAOSFC010000077 DAOSFC010000000
VERSION     DAOSFC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13855_length_5286_cov_5.671382"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..>1322)
                     /locus_tag="PK791_03005"
     CDS             complement(70..>1322)
                     /locus_tag="PK791_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOH92314.1"
                     /translation="VLGAFIPIVAFFIGKEIKPSIAWLSAILFCFLPSFVRHSHYATP
                     DIPLTLWIMLAMLFAIKYAKGAKESTLWLAIFFCAVSTADKYPGVISLVMVAGAVLWR
                     FLDKRKAEGKFDAKGFVGKGFLCFFIFVIALFLVAPFLFIRYKEVIEALIFEGTGGHL
                     GRDGLSYPLRVLTFLNYFLTEAGWLVTAFSVLGIVGFIRDKAKYALFLLFSLVFLLAI
                     SVVGPHHERWALPVYIGLLFCAAYGIDFLIQLSQRKSFVVLAKAFAVITLLLFAIAGL
                     SQSFWLKLQDTRVKGLYYLEKMQIAESDTYYDGYTPFAPNYFPEITKQDPETRLQRDY
                     AVLSSFYYERYHVLDDEHQENRFYADIRQQGELMHTIMGEPCPYKIKDQIRLFNYWIK
                     GRLLKQETETLFTGPDLYFYRLKP"
     assembly_gap    1323..1422
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1543..2316
                     /locus_tag="PK791_03010"
     CDS             1543..2316
                     /locus_tag="PK791_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOH92315.1"
                     /translation="MASLIGKFFLQFLEPLAIVGLFLFISILIVRSKAKGSQFFLIVA
                     LLLVAIFGNSFFANWLSRSMEWRHMPVDVTTSKADAIVVLVDGVYPAESPRLRVELGE
                     ESDRLLYATALLQRGAAQGILLVGSAEETDAASKLLLELGLPEQVIFQSQSSDDIVEI
                     VSTSAGALQTVGAKSVLLVTSALEMDRMRFAFEQSGLEIIPAPCDYHITKAYWDSSME
                     LSLPNVLTHLMPNSESFARSVAALREYFSLAFYRMRVIL"
     gene            2514..3899
                     /locus_tag="PK791_03015"
     CDS             2514..3899
                     /locus_tag="PK791_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOH92316.1"
                     /translation="MKIQKIVLTVVLLLSMVLSACQPAPVTPEPVTPETEPDLYPVEE
                     QTMPEDLTQAEDTYPILDENKDTDGAVGNSAYISDPKASAEELQKLAHASNQFAIDLY
                     QRLVDKEGNLIYSPFSIYQALLMTYAGAEGETAQQMMDVLGVTDNNEIHNVMNALKLT
                     LQAEPTYTIEGMQPLIFNIANALWVQKDFHFEQTFLDKLMANYAAGLALVDFNKPDEA
                     RDLINHWVEVRTNEKIKELIPTGMLNEMTRLVLTNAVYFKGAWTNRFDSAQNTQEEFT
                     SLNGAKSKVEMMNNSFTGAAFVEKDYSVVSLPYEGGNFAMMAVLPEDFASFQTTMNAD
                     LLNEILEKLTESHTMVHLTMPKFSFESSIDLGETLPAMGMSDAFDINNADFSGMTGGK
                     DLYISDVVHQAFIDVNEDGTEAAAATMVSMAPTSMPGEAITLRLDRPFIYLIYNTQTN
                     AVVFMGHVVAP"
     gene            3999..5273
                     /locus_tag="PK791_03020"
     CDS             3999..5273
                     /locus_tag="PK791_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOH92317.1"
                     /translation="MKTTRTIWTTIMISILLLTSCVPLTKLTGPKRVTGNLAYIENPQ
                     ATPEEFAQIASANNQFAFSLYQQLSKAENNLVFSPYSIHQALTMTYAGARTETEAQMS
                     DVLGFQGLEDPHRLINALNRKLVDKPAYLNKDYQPVEINIANALWLQKDYPLKQDFVD
                     LLSSNYDAGLYLLDFSKSNRAVAIINNWVSDQTNNKIKDLLSPANVNDTTRLVLTNAI
                     YFNGAWLDKFRPELTGSAPFFSLDGTQKNVDMMSGTFFVKALVNDELSAVILPYYGRN
                     YAMLLLMPEDFSAFQQGFNADSFEKLLADLQETYPQVNLEMPKFKIETHKSLAEQLEA
                     LGMPNAFDFRADFSGMTGSRDLIIDDVIHQAMIDVAEEGTEAAAATAVLMAEGGVGMD
                     SRIDITFNRPFIYAIYNTETNAIVFMGHFLNP"
BASE COUNT         1486 a         1219 c         1177 g         1304 t
ORIGIN      
        1 ccggaactct atgagtatat ctttggcacg tcggatttga cggcgtaaat taaactcagc
       61 ttatttgaat tagggcttga gtctgtaaaa atagagatcg ggaccggtga agagcgtctc
      121 ggtctcttgt tttaatagtc tgcctttaat ccagtaattg aacaagcgga tttggtcttt
      181 tatcttatac gggcaaggtt cacccataat ggtgtgcatg agttcaccct gctgacgaat
      241 atccgcataa aaacggtttt cctggtgctc atcatccagc acatggtaac gttcatagta
      301 aaacgaactc aaaacagcgt aatcgcgttg cagccttgtt tccgggtctt gtttggtgat
      361 ttccggaaag taatttggcg cgaagggggt gtaaccatca taataggtgt cgctttccgc
      421 gatttgcatt ttttcaagat aatatagccc tttaacgcgt gtgtcttgaa gcttgagcca
      481 aaatgattga ctaaggcctg ctatagcaaa gagtagcaag gtgattactg caaaagcttt
      541 tgccaaaacc acgaatgatt ttcgctgact taactgaatt aaaaagtcaa ttccgtaggc
      601 cgcgcagaag agcaatccga tataaaccgg aagcgcccag cgttcgtgat gggggccgac
      661 aactgaaatt gccagcagaa agacaagact aaaaagaaga aaaagcgcat attttgcttt
      721 atcgcgaatg aagccaacaa tacccagaac tgaaaacgca gttaccagcc agcccgcttc
      781 agtcaggaaa tagtttagga aggttaagac gcgtaaaggg taactgagac catcccggcc
      841 gagatgtccg cctgtgcctt caaagataag cgcctcaata acttctttgt atctgataaa
      901 caggaagggc gcgaccaaaa acaaggcgat aacgaagata aaaaagcaca aaaagccttt
      961 ccccacaaag ccttttgcgt caaacttacc ttcagctttg cgtttatcaa gaaatcgcca
     1021 aagcaccgcg cccgcgacca tgaccagact gattacgcca ggatatttgt ccgcggtgct
     1081 gaccgcgcag aaaaagatag ccagccaaag cgtggactct ttggcgcctt tggcgtattt
     1141 tatcgcaaac agcatcgcca gcataatcca cagggtcaat gggatatcgg gagtggcgta
     1201 atgagaatga cgcacaaaag agggtaagaa acagaagagt atcgcggaaa gccaggcgat
     1261 tgatggttta atttcctttc cgataaaaaa ggcgacaatg ggaataaaag cgcccaaaac
     1321 agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcataaat cgccaaacgg
     1441 gtattttaaa acgccatcag tataccactt aaaagaataa aacagacgct cgggcacatt
     1501 acttgcaact atagtgtcag gtttttagcg aaggagtcga ttatggcaag tcttatcgga
     1561 aaattcttct tacaatttct tgaacccctg gcgattgtgg gtttgttttt gtttatcagt
     1621 atcttaatcg ttcgttcaaa agcgaaaggt tcgcaatttt tcttgattgt cgccttgtta
     1681 ttagttgcca tatttggcaa cagtttcttc gccaactggc ttagccgttc aatggaatgg
     1741 cgccacatgc ctgttgatgt gaccacaagc aaagctgacg cgattgtggt gctggtggat
     1801 ggcgtctatc ccgctgaaag tccccgcttg cgtgttgaac tcggcgaaga atccgatcgt
     1861 ctgctttacg ccaccgcgct tttacaacgt ggcgcggcac aaggcatttt gcttgtcggc
     1921 agtgcagaag aaaccgacgc ggccagcaaa ctgcttttgg aactgggttt gcctgaacag
     1981 gtcattttcc aaagccaaag ttcagatgat attgttgaaa tcgtgagtac cagtgcgggc
     2041 gctttacaaa ccgtgggcgc gaaaagcgtc ttgctggtga cttccgccct ggagatggat
     2101 cgcatgcgct tcgcttttga acaaagcgga ctcgaaatta tccccgcgcc ctgcgattat
     2161 cacatcacca aagcctactg ggacagttcc atggaattgt ctctccccaa cgtgctgacc
     2221 catctgatgc ccaacagcga aagcttcgcg cgttctgtgg cagccttgcg cgagtatttt
     2281 tctctcgcgt tttaccggat gcgcgtcatc ctttaatgtg tttgtatcgc tgaccctcta
     2341 tcagcgacaa aacttccgcg taaagtctgc cagcctgatg gggtggcaga ctttagttta
     2401 agcacctggt gtcttagata ttccctgaca attttggcaa aaataccggg aacaattagt
     2461 caccattaaa cgtcttacaa gtgaaaggct tattgtatag ataaggagaa agaatgaaaa
     2521 tacaaaaaat tgttttaact gttgtcttgt tactgagtat ggttttgagc gcctgccaac
     2581 ccgcaccagt cacgcccgaa cccgtcacac ccgaaactga acccgactta tatcctgtgg
     2641 aggagcaaac catgcccgaa gaccttacac aagcagaaga tacctaccct attcttgatg
     2701 aaaacaaaga cactgatggc gctgttggca attcagctta tatcagcgac cccaaagcca
     2761 gcgccgaaga actgcaaaaa ctggcgcacg ccagcaatca atttgccatt gacctttatc
     2821 agcggcttgt ggataaagaa ggcaacctga tttattcacc ctttagcatc taccaggccc
     2881 tgcttatgac ctacgctggc gcggaaggcg aaaccgccca gcagatgatg gacgtcctgg
     2941 gtgtcacgga taacaatgaa attcacaacg tgatgaacgc cctcaaactg accttgcaag
     3001 cagaacccac gtataccatt gaaggtatgc agcctttaat cttcaatatt gccaacgccc
     3061 tgtgggtgca aaaggacttc cactttgaac agaccttttt ggataagctt atggctaact
     3121 atgccgcggg tttggctttg gtggatttca acaagcccga tgaagcgcgc gacctcatca
     3181 atcactgggt ggaagtacgg accaatgaaa agattaaaga gcttatccca acgggaatgc
     3241 tgaacgaaat gacccgactg gtactcacca acgcggtcta tttcaagggc gcctggacaa
     3301 accgttttga ctccgctcaa aatacgcaag aggaattcac cagtctcaat ggtgccaaaa
     3361 gcaaggtaga aatgatgaat aacagcttta ctggggccgc tttcgtggaa aaagattaca
     3421 gcgtcgtgag tttaccctat gagggtggca actttgccat gatggcagtt ctgcccgaag
     3481 actttgccag cttccaaacc acaatgaacg ccgacttact caatgagatt ttggaaaaat
     3541 tgacggaaag ccacaccatg gttcatctca ccatgcccaa attcagcttt gaaagcagta
     3601 ttgatttggg cgaaaccctg cccgcgatgg gcatgtccga cgcctttgat attaataatg
     3661 cggatttctc cggcatgacc ggcgggaaag acttgtatat ctcggatgtg gtgcatcagg
     3721 cttttattga tgtcaacgaa gatggcaccg aagctgccgc ggcaactatg gttagtatgg
     3781 cgccaacaag tatgcctggt gaagcaatca ctttacgcct tgaccgcccc tttatttatc
     3841 tcatctacaa cacccaaacc aacgccgtag tctttatggg ccacgtcgta gcaccttagc
     3901 attcaacttt agttaaagac agcgcttcaa aacaaagaga agcgctgtca aatctttgaa
     3961 cacaagccgt ctgaacttgt agctaaaaag gagaagaaat gaaaacaaca cgaacaatat
     4021 ggacgacaat catgatttca atccttttgt taacatcatg cgtccctctg acaaaattaa
     4081 caggccccaa acgcgtaaca ggtaatctgg cttacatcga aaacccccag gccacaccag
     4141 aagaatttgc tcaaattgcc agcgctaaca atcagttcgc cttttcgctt taccagcagc
     4201 ttagtaaggc tgaaaacaat cttgttttct cgccatatag tattcatcag gcgctgacca
     4261 tgacttatgc cggcgcgcgg accgaaaccg aagcgcaaat gtccgatgtc ttgggatttc
     4321 aggggcttga agacccgcat cgattaatca acgcgcttaa ccgcaaactc gtggataaac
     4381 cagcgtattt gaataaagac tatcagcctg ttgaaataaa cattgccaac gccttatggc
     4441 tgcaaaagga ctacccgctc aaacaggatt tcgtcgattt gctgtcgtca aattacgatg
     4501 ctgggctgta cttgcttgat ttttcaaaat caaatagagc agttgccatt attaataact
     4561 gggtttcgga ccaaaccaac aacaaaataa aagatttact aagccccgcc aacgttaacg
     4621 atacgacgcg tttggtttta accaacgcta tctattttaa tggtgcctgg ctggataagt
     4681 ttagaccaga attaactggc agcgcgccgt tttttagcct tgatggcaca caaaaaaatg
     4741 tggatatgat gtcaggcaca ttttttgtca aagccctggt gaatgatgaa ctttccgctg
     4801 taatcttgcc ttattatggg agaaattatg ccatgctcct gctcatgccc gaggacttca
     4861 gcgcgtttca gcaggggttc aatgccgata gtttcgaaaa actactcgcg gatttacagg
     4921 aaacctaccc gcaagtgaac ttagaaatgc ccaaattcaa aattgaaaca cacaaaagcc
     4981 tggctgaaca attagaagct ctgggtatgc cgaatgcttt tgattttaga gcagattttt
     5041 ccgggatgac aggtagcaga gatttaatta ttgacgatgt tattcatcag gcgatgattg
     5101 acgtcgctga agaaggcact gaagccgctg ccgctactgc tgtgttgatg gcagaaggag
     5161 gcgtgggcat ggattcccgc atagatatta cttttaaccg accctttatc tacgcgatct
     5221 ataataccga aacaaacgct attgtcttta tggggcactt cctaaaccca taaacagctt
     5281 tgactt
//
LOCUS       DAOSFC010000078         5213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_14128_length_5213_cov_4.299535, whole genome shotgun sequence.
ACCESSION   DAOSFC010000078 DAOSFC010000000
VERSION     DAOSFC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14128_length_5213_cov_4.299535"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="PK791_03025"
     CDS             <1..1321
                     /locus_tag="PK791_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH92318.1"
                     /translation="VWGRGALDTKCTVIGILEAVNNLLREGFQPERTVYLAFGHDEEV
                     SGTYGAKVIAKLLEERGIQLAFLLDEGGYISTGFLPGVNVPVGLIGVAEKGHVTLKLR
                     AEVKGGHSAYPPKDTAIGALSLAIAALEANPFPQSLDMVEFMMSFVGKQLPFGLRLAL
                     ANPWLFGNAIKRKASTNRHLNAITRTTLSPTIIHAGDAENVLPSVAEAIINIRIMPGE
                     TLASTYEYVRDLVADDVVSVLPAYGDQLMGDHSWDPVEVSDIDSPHFHILYQLIRAAF
                     PGAQATPILMPGATDARHYRNVCKRTFRFTPIMVSPEESNRTHGVDERLSFENAGRMV
                     AFFQLLIQNMSALSAEEELAYSAPDDVLEETFEQKAIREMNEALPTRPMRQTPPATAV
                     FEPAEPEPVDDDTDTDLDVSFDDAFDDTFDDTFDDDAPLKTKPLKKD"
     gene            1327..2805
                     /locus_tag="PK791_03030"
     CDS             1327..2805
                     /locus_tag="PK791_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92319.1"
                     /translation="MPKAKNNNKYLAIVLVLAVVFFSIIVLMECILLRGINSGQFLSQ
                     EPPGLAENLPAQSPQASPSPVPASAEPEESEPTPEAIVTPTPEPTPEPTPSVTLEPEI
                     AQMMDQIEEQISEVRLLEMSERIPRKVYSRDELREFVREEMLEDLDEEAESNNVRFYN
                     LLGLLPEGLEMKTLLEDLYSEQIAGFYTIEDREMVVVAGDEFGISERLTYAHEYVHAL
                     QYANFDIENGLGYNEDDCQEDSERCLAIQALIEGDANLSQFLWFENHATRADIMELIL
                     SFEETESHVLDSAPAYLTTSMMFPYEQGTAFVQYLYSINGFDTINEAFTTTPPVSAEQ
                     IMFPERYPDDLPVKVDLPDVLAALGEDWEIDHDGVVGAWDSYLMLSKGYDERFQIDED
                     VALAAVEGWGGDAYALLKNESQDEFLFIMNTTWDTEEDQQEAWNAFSAMLKLRFGSPG
                     YDGLFSKDGTFARLFKTDEKGFVLILSEMLDGLESLYDFLSE"
     gene            2810..3526
                     /locus_tag="PK791_03035"
     CDS             2810..3526
                     /locus_tag="PK791_03035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH92320.1"
                     /translation="MPNKPAFKSSLDRKKIKALLFDIDGTLSDSDDEMVASIYARMAW
                     LSPPFQEIRLHKFARQLVRFFQAPANRFLAFLDHIGIDSHLARFLDHRARKKKAVPED
                     FPVIPGVRPMLDALHKRYPFAVVSARNSVTVEKFLRVNDLHSYFDIVISSQTCEKTKP
                     FPEPLLFAASELGVPIENCLMIGDTLTDVRAALAAGAQSLSVLCGFGTEKELRKSGTH
                     HILHSTSELADLLNEQNISE"
     gene            complement(3584..4933)
                     /locus_tag="PK791_03040"
     CDS             complement(3584..4933)
                     /locus_tag="PK791_03040"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92321.1"
                     /translation="MMNQDPNTATEQPSVADEAIIAPDAQPELPDKWLKNYLPMGVGQ
                     ILSMLGSGLVHFALIWFITKETGSAITLLMATITGVLPSVILGPFAGALVDRWNRKLT
                     MIISDGVIALATLLLAFLFAIGKIQIWHVYAILFIRSVGGIFQGPAMSASITLMVPRE
                     HYTRLAGVSHAVDGIITIVSPPLGALLMSLLPIQAVIAVDIVTAAMAILLLFAVKIPQ
                     PPKQKSEKVATSIKTVLTDVKDGFAYVISWRGIFAAMIGGTLINMAATPSFSLLPLHV
                     DQFFQKGSDGLAFLYSALGVGSILGGLALSVWGGFKRKILTIAMGIFSMGLCMLAFGF
                     LPAQGYTVAIFLIAFFGIANVFANGPLSALFQDKIPSELQGRVFTVMGSLVQATVPIG
                     LLIAAPIAEWLGISAWFIIGGIACVVLAIVMLLYKPMATLDWQGPGGEIMPEYQDRV"
BASE COUNT         1350 a         1389 c         1262 g         1212 t
ORIGIN      
        1 cgtgtggggg cggggcgcgc tggacaccaa atgcaccgtg atcggcattt tggaagcggt
       61 gaacaatctc ctgcgcgaag gatttcaacc ggaacgcacg gtttatctcg cctttgggca
      121 cgacgaagaa gtttccggca cttatggagc caaagttatc gcgaaacttt tggaagagcg
      181 tggcattcag ctcgcctttt tactcgacga aggtggttat atcagcacgg gtttcctgcc
      241 gggcgtcaat gtgcccgttg gactaatcgg tgtggctgaa aaagggcatg taacgctcaa
      301 actgcgcgcg gaagtgaaag gtggccatag cgcctacccg cccaaagaca ccgccatcgg
      361 cgcgctgagc ctggccatcg ccgcgcttga agctaatccc ttcccgcaaa gcctggacat
      421 ggttgaattt atgatgagtt tcgtgggtaa gcaactgccc tttggtctgc gcttggcact
      481 cgccaacccc tggctctttg gcaacgcgat aaagcgaaaa gccagcacta accgccatct
      541 gaacgcgatc acccgcacga cactctcgcc gaccattatt cacgccggcg atgccgagaa
      601 tgtcctgcca tcggtggcgg aggccatcat caacattcgc attatgcccg gcgaaacgct
      661 ggcaagcacc tatgaatatg tgcgcgattt ggttgccgat gatgtggtta gcgtgttgcc
      721 cgcctatggc gatcagctta tgggcgacca cagctgggac cctgtggaag tttcggacat
      781 tgattcgcca cattttcaca ttctgtatca gctcatccgc gccgctttcc ccggcgcgca
      841 agccacgcct attctcatgc ctggcgcgac cgacgcgcgg cattatcgca acgtgtgcaa
      901 gcgcactttc cgctttacgc caatcatggt tagcccggaa gaatccaacc gcacacacgg
      961 cgttgacgaa cgcctttcgt ttgaaaatgc cggccgaatg gtggcgttct tccagttgct
     1021 tatccaaaac atgagcgctt tgagcgccga agaagaactg gcatatagcg cccctgatga
     1081 tgtgctggag gagacttttg aacagaaagc tattcgggag atgaacgaag ccttgcccac
     1141 gcgtccgatg cgccagacac cccccgccac tgctgttttt gaacctgcgg aacctgaacc
     1201 cgttgatgac gacacggata cggacctgga tgtcagcttt gacgatgctt ttgacgacac
     1261 ttttgacgac acttttgatg acgacgcccc gctcaaaacc aaaccactga agaaagacta
     1321 atctttatgc ccaaagctaa gaataacaac aaatatctcg ccattgtgct cgttttggca
     1381 gtcgtctttt tctccatcat tgtactgatg gaatgtattt tgctgcgtgg gattaactcg
     1441 ggccagtttc taagccagga gccaccagga cttgcggaaa acctgccagc ccagtccccg
     1501 caagccagcc caagccctgt accagcaagc gctgaacccg aggaaagcga gccaacaccc
     1561 gaagccatcg ttacccccac gcctgaacca acacccgagc caacgccatc ggtcacactc
     1621 gaacccgaaa tagcgcaaat gatggaccaa attgaagaac aaattagcga agtgcgcctt
     1681 ttggagatga gcgaaagaat cccgcgcaag gtctatagcc gagacgaact gagagaattc
     1741 gtgcgcgaag aaatgcttga agaccttgat gaagaggccg aatcaaataa cgtgcgcttt
     1801 tataaccttt tggggcttct gcccgagggg cttgaaatga agaccttgct tgaggatctt
     1861 tattcagagc aaatcgcggg cttttacaca attgaagatc gcgaaatggt cgttgtagca
     1921 ggcgatgaat ttggcatcag cgaacgcctt acctacgccc acgaatacgt tcacgctttg
     1981 caatatgcta attttgacat cgaaaacggt cttggctaca acgaagacga ttgccaggaa
     2041 gactccgagc gttgtctggc cattcaagcc ttgattgaag gtgacgccaa cttaagccag
     2101 ttcctgtggt ttgaaaacca tgccacacgc gcggatatca tggaactcat tctcagtttc
     2161 gaagagaccg aaagccatgt tttggactcc gcgccagctt atctgaccac atccatgatg
     2221 ttcccttatg aacaaggcac cgcttttgtt cagtaccttt atagcataaa cggctttgac
     2281 accatcaacg aagcattcac caccacacca cccgtcagcg cggagcaaat catgttcccc
     2341 gagcgctatc ccgatgacct gcccgtcaag gtcgacttgc ccgacgtttt ggccgcgctg
     2401 ggcgaggact gggaaatcga ccacgatggc gttgtgggcg cctgggacag ttatttgatg
     2461 ctcagcaaag gctacgatga gcgctttcaa atcgacgaag atgtcgccct ggccgccgtt
     2521 gagggctggg gcggtgacgc ctacgccctt ttgaaaaacg aaagccagga cgaatttctc
     2581 tttatcatga acacgacctg ggatacagaa gaagaccaac aggaagcctg gaacgctttt
     2641 tcagcgatgc tcaagctccg gtttggcagt ccaggttatg acggtttatt cagcaaagat
     2701 ggcaccttcg cgcgcctttt caaaaccgat gaaaagggtt ttgtgctcat cctgtctgaa
     2761 atgctcgatg gtctcgagtc gctttatgat ttcctgagtg agtaaaaaca tgcccaataa
     2821 accagctttc aaatccagcc ttgaccgcaa aaaaataaag gctttgctct ttgatatcga
     2881 cggcacgctt tcagacagcg atgacgaaat ggtggcttct atttacgcgc gcatggcatg
     2941 gctcagtccg cccttccaag agatacgcct gcacaaattc gcccgccagc tggtgcgttt
     3001 ttttcaggcg cccgccaacc gctttttggc gtttctggac catatcggca tcgacagcca
     3061 cctggcacgg tttttagacc accgcgcgcg aaaaaagaaa gctgttcctg aagatttccc
     3121 ggttattcct ggcgtgcgcc ccatgctgga tgccctgcac aaacgctatc cctttgcggt
     3181 tgtttccgcg cggaacagtg taactgtgga gaagtttttg cgcgttaacg atttgcacag
     3241 ctatttcgac attgttatca gttcgcagac ctgcgagaaa accaagcctt tcccggagcc
     3301 gctgctattc gccgcgtcgg aactgggtgt gcccatcgaa aactgcctga tgatcggcga
     3361 cacgctaacc gatgtccgcg ctgctttggc tgccggagcg cagtcgcttt ctgttctgtg
     3421 tggcttcggc accgaaaaag agttgcgcaa aagcggcacg catcatattc tgcatagtac
     3481 gagcgaactg gcagacttgc tcaacgaaca aaatattagc gagtagatgt tttcccgagg
     3541 ttttcccggg aaattaaacg aataacgggg aagagagaac agactaaacc ctgtcctgat
     3601 attctggcat tatttccccg ccaggacctt gccagtcaag tgttgccatt ggtttataca
     3661 agagcatgac aatggcaagc acaacacaag ctataccacc gataatgaac caggcggaaa
     3721 tgcccagcca ttcggcgatt ggcgcggcaa tcagcaagcc gatcggcact gtggcttgaa
     3781 caagcgaacc catcaccgta aacacgcgcc cctgtaattc tgacgggatt ttatcttgaa
     3841 aaagcgcgct aagtggcccg ttggcaaaca cgttggcgat accaaaaaac gctatgagaa
     3901 aaatagctac ggtatatccc tgggcaggaa ggaagccaaa tgcaagcatg caaagaccca
     3961 tcgaaaaaat gcccattgct atggtcaata tcttgcgttt aaagccaccc catacgctta
     4021 gagccagtcc acctaaaatc gagcccacgc ccaaagcgga atagagaaat gccagtccgt
     4081 ctgagccctt ctgaaaaaac tgatcaacat gcagcggcaa aaggctgaag gatggcgtgg
     4141 cggccatgtt aatcagcgtc ccaccaatca tggcggcaaa aatacccctc cagctgataa
     4201 cataggcgaa gccgtctttg acatcggtca agactgtttt gatgctcgtg gctacctttt
     4261 ccgatttctg cttgggaggt tgcgggattt taactgcaaa caataaaaga atagccatcg
     4321 ctgcggtgac aatatcaact gctatcaccg cctgaatagg taacaaagac atcaacaatg
     4381 cgcccaaagg tggtgacaca atggtgataa tgccgtccac agcatgcgaa acacccgcta
     4441 atcgggtgta atgctccctg ggaaccatca gcgtaattga agcactcatc gccggtccct
     4501 ggaagattcc ccccacggaa cgaatgaaga gaatagcgta aacatgccaa atttgaattt
     4561 ttccaatagc aaacaaaaag gctaataaaa gcgttgccag ggcgatgaca ccatctgaga
     4621 taatcatggt taattttcgg ttccagcggt ctaccaacgc gccagcaaac ggacccagga
     4681 taacactggg caagactccc gtgatggttg ccataagaag cgtaatcgct gaaccggttt
     4741 ctttggtaat aaaccaaatc agcgcgaaat gcaccaagcc tgagccaagc atggaaagga
     4801 tctgccccac acccatcggc aggtagtttt taagccattt gtctggcagt tcgggctggg
     4861 catccggcgc gataattgct tcatcagcca ctgatggctg ttccgtagcg gtgtttgggt
     4921 cctggttcat catctcttgc tccttaacaa aggctattat aaagcaattt cgccccatga
     4981 tgattttttc tcatcaatgc ctgttttttc atacatgcat ttgttcaata atattttcag
     5041 cataatatgt catttcttca agattaaatc cccttattaa tgtcatttgt tcaatatttc
     5101 aagcgcctta ataacgcatt tcttcaatat ttagtgttat tggctttcag aacattcttt
     5161 ttttacaatc ccacataaaa tcgctaatcg ttgtaaaata agacatcacc tca
//
LOCUS       DAOSFC010000079         5195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_14192_length_5195_cov_5.113035, whole genome shotgun sequence.
ACCESSION   DAOSFC010000079 DAOSFC010000000
VERSION     DAOSFC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14192_length_5195_cov_5.113035"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PK791_03045"
     CDS             <1..720
                     /locus_tag="PK791_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH92322.1"
                     /translation="LKALVSGGYPVLIERGHTDPQDGWMGHYSIIHAYDDSTQTVTSN
                     DTLLSTITIPYEELMRDWRHFDGIYVVIYPADRDADVLARLGVHADESENLRLSLAEI
                     EARIPHETGRELFFAWYSKGQILVAMGEYMSAAQAFDQAFGVYATLDMGARPWRMTWY
                     QIGPYIAYYHSGRYQDTLALAQQTLDNSREPKALPESYYWAGMAAKAMGMSDDARVWF
                     EKALEYHPGWDVVLEAMADLP"
     gene            complement(763..1578)
                     /locus_tag="PK791_03050"
     CDS             complement(763..1578)
                     /locus_tag="PK791_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92323.1"
                     /translation="MNRSTPEAEQFFGLTIDDVWYDDIALEDVLDGLKNLEVRPMVRI
                     VMSSEIEPVDYINLFKSVAQYADIMASPVDSYVMNSYKDTESYLKRFTDSYESLAPYV
                     SIWEVGNEINGTEWIKQSPELIVNKVSSVAKFLKSKGEKIALTLYCTDSPHQDMITWA
                     EKYIPEELANIVDYCLVSYYEDDNDGYLPDWESVFKELGDIFPVSFLGIGECGNTAPD
                     ATQESKIEMAKRYYQMPKPHERFVGGYFWWNWVTDCIPYKDNAVYDAIKSFGH"
     gene            1848..2735
                     /gene="cdaA"
                     /locus_tag="PK791_03055"
     CDS             1848..2735
                     /gene="cdaA"
                     /locus_tag="PK791_03055"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOH92324.1"
                     /translation="MSELISNFLFIWQRFTWESLIDILLVAAFFAVFLYLLRDTEGQT
                     LLRGALVVVIAITLLTSMVNLPAFSWLVSTLTPVLILSIPVIFAPEIRRGLGRMGKIG
                     ELNIFGAKRSQRATEQLKKTLDAITDAVVQLSARKHGALIVIRRKQYLDQYYKNGILL
                     DADVTPQLLLQIFYPNTPLHDGAVIIEDNRIKAASCVMPLSSSGVLYVSVERSMGLRH
                     RAALGISEVSDAIAVVVSEETGTISIAQEGRLIRRLDSDRLRNSLHAFMTLQVTEIDK
                     KSRTGFLGLFRKKTTKGED"
     gene            2739..3755
                     /locus_tag="PK791_03060"
     CDS             2739..3755
                     /locus_tag="PK791_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOH92325.1"
                     /translation="MKKLLNKIISLLPIVLLSLVLAFFVWFSAVNSKDPTEEITYNKP
                     IPIEILGQNPQYTITEQSVSNVTVTIKAPRSVHNALANDMKLIRATVNISALQDGYAE
                     LEPEFLIGIKPAKLVDFNPKTVNLTIEKLITKTFDIVINQIGNLSTGLEARPARLDVR
                     EVQVTGPESRVSKITELLATVDMSTIIGSLNKQVDLIAVDEQGNIVDGVTINPSRVTV
                     NIPVLQRGGYKNVFLALVPLGSPAYGYQITKTEIIPPMVTVYTNSLSQIESVPDLIET
                     TPVNLNGRDKSFEQVVTLNLPAGVNLASDVEIIVKITIEPTITTKTIRGIPITLITRP
                     KDLM"
     gene            3755..3964
                     /locus_tag="PK791_03065"
     CDS             3755..3964
                     /locus_tag="PK791_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOH92326.1"
                     /translation="MLNITTVDVYLTGPTHLLAELTAQNLTVEIDLSDFKAGTHQVNP
                     AVILPAASINYLIVPDTVEVRLQPR"
     gene            3989..>5195
                     /locus_tag="PK791_03070"
     CDS             3989..>5195
                     /locus_tag="PK791_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOH92327.1"
                     /translation="MQKPVVVLVGRPNVGKSTLFNRLIGERLAIIDDIPGTTRDRLVA
                     EGNWAGYDFFVVDTGGIDPSKQRTQEPLSIGSKEFIRDIREQAEIAMAEADVILLMVD
                     AQQGVTQADLELVDILRRRQVSSDGTDPTPVLLVANKAESKSAWLGSMDFYSLGLGEP
                     YAISAIHGTGTGDLLDAVVAHFPHAIDDDTADESIKVAIVGKPNVGKSSLLNIMAGLD
                     HEYQGQAVVDGKAITGPSAERLLLFQEPALFPWMNIAENVAFGLAARGLPRCERIPVV
                     DRYLAMVHLEAFAHAHPHELSGGMRQRAALARALAVDPDLLLMDEPFAALDAQTRDML
                     HGELQEVWTTTGKTIVFVTHNVREALILGDRVLLMSARPGKIKKEFVFDLPRPRHFED
                     PGIAEAARVV"
BASE COUNT         1345 a         1265 c         1204 g         1381 t
ORIGIN      
        1 ctcaaggctt tggtttcggg tggctatccg gtcttaattg aacgtgggca taccgacccg
       61 caagacggtt ggatggggca ttacagcatc attcatgcct atgatgacag cacacaaacc
      121 gtgaccagca atgatacttt gttgagcacc atcacgattc cctacgaaga actgatgcgc
      181 gactggcggc acttcgacgg catttatgtc gttatctacc ctgctgaccg tgacgcggat
      241 gtgcttgccc gcctcggtgt gcatgccgat gaatcagaaa atttgcgctt aagtctggcg
      301 gagattgaag cgcgtattcc gcatgaaacg gggcgcgagc ttttctttgc ctggtatagc
      361 aaggggcaga ttttggtggc gatgggtgaa tatatgtccg ccgcgcaggc ttttgaccag
      421 gcttttggtg tatacgcgac cctggatatg ggcgcgcgtc cctggcgcat gacctggtac
      481 cagattggcc cttacatcgc ctactaccat tcaggtcggt atcaggacac cttagctttg
      541 gctcaacaga cgctggataa cagtcgcgag ccaaaagcct tgcccgaaag ctattattgg
      601 gcaggcatgg ctgctaaagc tatgggcatg agcgatgatg cccgcgtctg gtttgaaaaa
      661 gcgcttgagt atcacccagg ttgggatgtg gtgcttgaag caatggcaga tttgccatag
      721 tttgcctgcc tatgcgtgtt tacatgagca aaaattagat tgtcagtgcc caaagctttt
      781 aatggcatcg taaacagcat tgtctttgta aggaatgcaa tctgttaccc agttccacca
      841 aaagtatccc cccacgaatc tctcatgggg ttttggcatt tgataatagc gttttgccat
      901 ctcaattttg ctctcctgcg tcgcatctgg ggctgtattg ccacattctc ctataccgag
      961 aaatgagacc ggaaaaatat ctcccagttc tttaaaaaca ctctcccagt cgggtaaata
     1021 accgtcattg tcatcttcat aatagctaac caaacaataa tcaacaatat ttgccagctc
     1081 ttctggaata tacttttccg cccaggtaat catatcctgg tgtggtgaat cagtgcagta
     1141 gagtgtcagc gcaatctttt cacccttgct ttttagaaat tttgcaacag aagacacctt
     1201 attcacaatc agctccgggc tttgcttgat ccattccgtg ccgttgattt catttcccac
     1261 ttcccaaatg cttacatagg gtgccaggct ttcatacgaa tctgtaaacc ttttcagata
     1321 actttccgta tctttatagc tattcatcac ataggaatca accggactgg ccatgatatc
     1381 cgcgtattgc gctacagatt tgaacaggtt tatataatcc accggttcta tttcgcttga
     1441 catcactatt ctgaccatcg gtcttacttc aagattcttc aatccgtcaa ggacatcttc
     1501 caaggcgata tcgtcatacc agacatcatc gatggtgagc ccaaaaaact gttccgcttc
     1561 cggagtactc ctgttcacac tatcgcattg acaaagagag atggccaaga cagcaagcac
     1621 caaaacaaag ctaaccgtct tgtggaatat tgatctggtt gaatacattt ttttgtttcc
     1681 cataaaacac cgcttttgtg gaacgaatca attcttgtga cgttttaatc ctgcttaact
     1741 tgaataaaat tatacccttg cttaaatagt tcactttact gtgacacttt tttagcgaaa
     1801 tatacttaat aagggtatac tttatggtgg tataaggttg gaatagcatg tctgaattaa
     1861 ttagcaattt tcttttcatc tggcaacgtt ttacatggga aagcctgatt gacatcctgc
     1921 tggtagcagc gtttttcgcg gtttttcttt accttttgcg cgacacagaa ggacagaccc
     1981 ttttgcgtgg cgccctggtt gttgtcatcg ctattaccct gcttacttca atggtcaatt
     2041 tgcccgcgtt ctcgtggctg gtatccacgc tgacgccggt cttaatatta tctataccgg
     2101 ttattttcgc gcctgaaatc cggcgtggcc tggggcggat ggggaaaatt ggcgagctta
     2161 atatctttgg cgctaagcga agtcaacgcg ctacggaaca actcaagaaa acacttgacg
     2221 caataaccga cgcggttgta caactttcag cacgcaaaca tggcgcgctc attgtcattc
     2281 ggcgcaagca atatctcgac caatactaca aaaatggcat cctgcttgac gctgacgtaa
     2341 caccgcaatt attgttgcaa atcttttacc caaatacgcc cttgcacgat ggtgcggtca
     2401 tcattgaaga caaccgcatc aaagccgctt cctgcgttat gccactttca agcagtggtg
     2461 tgttatatgt ttcagtagaa cgttccatgg gcttacgtca tcgggcggcg ttaggcatca
     2521 gcgaagtgag cgatgcaatc gctgtggtcg tttcagaaga aacgggcaca atttcaatcg
     2581 ctcaggaagg ccggcttatt cgccgactgg acagcgaccg cttgcgcaac agcttgcacg
     2641 cttttatgac tcttcaggta accgagattg ataagaaatc ccgcacagga tttctcggtt
     2701 tattcagaaa gaaaacgacc aaaggggagg attaggcaat gaaaaaacta cttaataaaa
     2761 tcatttcatt attgccaatt gtcctgttgt cgttggtgtt agctttcttt gtttggtttt
     2821 ctgcagtcaa ctcaaaagat cccacagaag aaataaccta taacaaacct atccccattg
     2881 aaatactcgg gcaaaaccca cagtacacaa tcactgaaca aagtgtgagc aatgttaccg
     2941 taacgattaa agcaccacgt tcagttcata acgctctggc aaatgatatg aaactcatcc
     3001 gggcgacagt gaatatctca gcccttcaag atggatatgc cgagcttgaa cccgaattcc
     3061 tgataggcat taaacctgcc aaactggttg actttaaccc caaaacagta aaccttacga
     3121 ttgaaaaact cattaccaaa acttttgata ttgttatcaa tcaaatcggc aatttatcca
     3181 ccggacttga agcacgtcca gctcgtttgg atgtgcgaga agttcaggtt accgggcctg
     3241 aatcacgcgt gtcaaaaata acggagttac ttgccacggt agatatgagc accattatag
     3301 gaagcctaaa taaacaggtc gatttaatcg cggtggatga acaaggcaat atcgttgatg
     3361 gcgttaccat aaacccttca agagtgacgg taaatattcc tgttctgcaa agaggtggct
     3421 acaaaaatgt attcctggcc ctggttcctt tggggtcacc tgcttatggt tatcaaatca
     3481 ctaaaactga aattattccg ccaatggtca ccgtttacac aaacagcctc agccagattg
     3541 aaagtgtgcc tgatttgatt gaaaccaccc cagtcaatct caatgggcgg gataaaagtt
     3601 ttgaacaagt ggtaacgctc aacctgcctg ccggcgtaaa tttggccagt gacgttgaaa
     3661 tcattgttaa aataaccatc gaacccacaa tcaccaccaa gaccattcgg ggaattccaa
     3721 tcactttgat aacacgcccg aaggatttga tgtgatgtta aacattacta ctgtagacgt
     3781 gtatttgaca gggccaacac atttgctggc ggaattaact gcacaaaatt tgactgttga
     3841 gatagatcta agtgatttta aagccggcac ccatcaagtt aaccccgcgg taatattacc
     3901 agctgcgtct attaactatc tcattgtgcc cgatacggtt gaggttcgtc ttcaaccccg
     3961 ttaaggacat gaaattcaag gattaattat gcaaaaacct gttgtagtac tcgttggaag
     4021 acctaacgtt ggcaaaagca cgcttttcaa tcgcctgatt ggcgaaagac tggcgattat
     4081 tgatgatatc ccaggcacca cacgcgaccg cctggttgct gaaggcaact gggctggtta
     4141 tgactttttt gtggtagaca ccggcggtat tgaccccagc aaacaacgca cgcaggaacc
     4201 gctttcaatc ggctccaagg aattcatccg cgacattcgc gaacaagccg aaattgccat
     4261 ggcggaagcg gatgttatcc tgctcatggt agacgctcaa caaggcgtaa cccaggccga
     4321 cctggagctg gtggatattc tcagacgccg gcaagtaagc agcgatggta ccgacccgac
     4381 gcctgtcttg ctggtagcca acaaagcgga aagcaaatca gcatggttgg gttcaatgga
     4441 tttttactcc ctgggacttg gcgagcctta tgccatttcc gctatccatg gcacgggcac
     4501 gggcgatctg ctcgacgcag ttgtggcgca tttcccccac gccatcgatg acgataccgc
     4561 agatgaatcg attaaggttg ccattgtggg taaacccaat gtcggcaagt ccagtctcct
     4621 caatatcatg gctggtcttg accatgagta tcaaggacag gccgtggtgg acgggaaagc
     4681 gatcaccgga ccttcggccg agcgactcct gctctttcag gaaccagccc tctttccatg
     4741 gatgaatatt gcagagaatg tggccttcgg tctggctgcc aggggactcc cccggtgcga
     4801 gcggataccc gttgttgacc gctatcttgc catggtgcat cttgaggcat ttgcacatgc
     4861 ccatccccat gagctttcag gcgggatgcg ccagcgtgcg gcactggcgc gggccctggc
     4921 tgtggatcct gatcttcttc tgatggacga accctttgcc gcacttgatg cccagacccg
     4981 ggacatgctg catggcgagt tgcaggaggt ctggacgaca acaggcaaga cgattgtctt
     5041 tgtgacccat aatgttcgtg aggcccttat ccttggtgac cgcgttctgc tcatgtctgc
     5101 acgacccgga aagataaaaa aagaattcgt gtttgatctt ccaaggccgc gacattttga
     5161 ggatcccggg attgccgagg cggccagggt tgtgc
//
LOCUS       DAOSFC010000080         5177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_14263_length_5177_cov_4.672198, whole genome shotgun sequence.
ACCESSION   DAOSFC010000080 DAOSFC010000000
VERSION     DAOSFC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14263_length_5177_cov_4.672198"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PK791_03075"
     CDS             complement(<1..390)
                     /locus_tag="PK791_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HOH92328.1"
                     /translation="MRYKILGDNLPAVTIQFDQGESIYTQSGGMTWMTDNFNMETNMR
                     GGLGKSLGRMFTGESLFMVTYTALAPNAEMTLASSFPGEIKPIQLDGSMEYIAQKSAF
                     LCATPGVELSTHITRAGAGFFGGEGFIL"
     gene            complement(458..961)
                     /gene="rpiB"
                     /locus_tag="PK791_03080"
     CDS             complement(458..961)
                     /gene="rpiB"
                     /locus_tag="PK791_03080"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOH92329.1"
                     /translation="MKIAIASDHAGFLLKEQIKNYLDSRLIEYEDFGTHNEESTDYPR
                     YAELVAKAIQSHGFDLGILVCGTGIGMSIAANKFHGIRAAVCSEPFSAILSRRHNNSN
                     VLCMGSRVVGSGLAMLITEGWLNAAYEGGRHERRVGMITRMEQRNAADVLQINSTDDT
                     QPFTKGE"
     gene            1098..2120
                     /locus_tag="PK791_03085"
     CDS             1098..2120
                     /locus_tag="PK791_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH92330.1"
                     /translation="MILVTGGAGHIGNVLVRDLVEKGERVRVLALPGEDVGSLDGLNV
                     EIVRGNILNMEQLREAMSGARLVFHLASFVALTADKYPLMRQVNINGTANVISLCKEL
                     GVERLVYTSSIHAFERVPEGIVVNEELSFDTENPDGLYDRTKAEATLLVLDAVKEGLD
                     AVVVCPTGVVGPYDFKLSELGEMIITWMKKRASVSTDGAYDFVDVRDVSKGHILAAEK
                     GLSGEVYILGGERLTIKAFRAMVQAANSIKTMAINIPAKLALFVAPIAEFFYKLFKKR
                     PKFTTYSIKTLLSNSHISYKKAIHELGYQPRQLKETIKDTVEWLKMNQSKLQHGLRMT
                     SVKPTV"
     gene            complement(2151..2990)
                     /gene="mutM"
                     /locus_tag="PK791_03090"
     CDS             complement(2151..2990)
                     /gene="mutM"
                     /locus_tag="PK791_03090"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOH92331.1"
                     /translation="MPELPEVETIARNLAKGDEQTPSVLGQRIVSSSVFWAKTVAEPD
                     LLSFHSAVKNQSVLNVSRRGKFLIFDLDHVFFLIHLRMSGDLIMRYADMPLPLSKPLV
                     PHDHVYLHFADNWHLAFNDTRKFGRVWLTPDPNHILSKLGPEPFDPKLTDVNFADKLR
                     KHKRQLKPLLLDQSFIAGLGNIYTDEALFEAGLHPKRQSNSLSLDDSSHLLRAIRKVL
                     RLGIEHNGASIDWAYKGGGFQNYFQVYQQTGNPCPRCGNPITRTVIGQRGTHFCERCQ
                     PLI"
     gene            complement(2990..3979)
                     /locus_tag="PK791_03095"
     CDS             complement(2990..3979)
                     /locus_tag="PK791_03095"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOH92332.1"
                     /translation="MTSQQPDFGMRYGNIRLFAGTASMELAQEIADYLGLELCGRDVI
                     EFTNENLFVKLHASVRGQDCYIIQTTSMPVHRNLMEMLIMAQTLKLDSAGRITLVFPY
                     MCYGRSDRKDQPRVPITAKLVCEMMEVAGADRYITMDLHAGQIQGFFSIPGDVLSAYH
                     IIKPYLADLLPDLTNPVILTADLGFAKNGRRYAMGLDVPLSFIEKRRIDNHDHTEALN
                     LIGDVKHRDVILIDDEVDTGGSICEAVETAKLNGARDVYVAFVHPVLSSNAVKRLTAL
                     PVKQFITTNTIPLSDNQKAAFGERLKVLSIGPLLGEVIKRTNQSTSVGVMFNE"
     gene            complement(3984..4559)
                     /locus_tag="PK791_03100"
     CDS             complement(3984..4559)
                     /locus_tag="PK791_03100"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOH92333.1"
                     /translation="MAILYGENVRLRATERTDIPTFQRWINDPEVVEFLSNIFPYSLE
                     AETAWFESVLKRPMAEHPLVIDLRLTDSKNGKSEWLAIGNCAFMGITDIHRSAEVGIF
                     LGEKEYWSQGYGTEAMRLMLKFGFEDLNLHRIWLRVLESNHRARKSYLKSGFLEEGRF
                     RKAEFRHGFYNDIILMSILQEEWFANKQKEG"
     gene            complement(4581..>5177)
                     /locus_tag="PK791_03105"
     CDS             complement(4581..>5177)
                     /locus_tag="PK791_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92334.1"
                     /translation="MAFCENSLAYAYTAVKEKHGEQIKETFDVEIEVPQTPFPRLTMS
                     EAYAILKDMDYKLPPEKKGDLDPGAERALGEYVKNTYGNDFVFVTDWPATVRAFYHMR
                     HADQPLLTKSFDLIGKGLELTTGAQREHRYEVLKAQAIEKGLGTELLQGYFDYFRYGC
                     PPHGGLGLGLNRLLMVMLNLPSIRDAVYLFRGPNRLEP"
BASE COUNT         1367 a         1081 c         1313 g         1416 t
ORIGIN      
        1 caggatgaag ccttcgccac cgaagaaacc cgcaccggca cgggtaatgt gggtgcttag
       61 ttcaacgcca ggagtggcgc acaagaaagc acttttttgg gcgatatatt ccatgctgcc
      121 atcgagttgg atgggtttga tttcgccagg gaatgaggaa gccagggtca tttcagcgtt
      181 aggtgcaagt gcggtatatg taaccatgaa aagcgattca ccggtaaaca tacgtccaag
      241 gcttttgccc agaccaccac gcatattggt ttccatattg aagttgtcgg tcatccaagt
      301 cataccaccg gactgtgtgt agatgctttc gccctggtcg aattggattg tgactgcggg
      361 gagattatct cctaatattt tgtatctcat aatgctcctt aaaattagtt tctaaacttt
      421 atcattgttt aaactctaac aaaacaatgt tatggattta ttcgcctttt gtgaatggtt
      481 gtgtatcatc ggtagaatta atctgcaaaa cgtcggctgc gttacgttgt tccatgcgcg
      541 taatcatgcc cacgcgacgt tcatgtctgc caccttcata ggcagcattg agccagcctt
      601 cggtaatgag catcgccagt ccactgccca caacgcgtga gcccatgcaa aggacattgc
      661 tattgttatg tcgacgggaa agaatcgcgg agaagggttc actgcatacc gcagccctga
      721 taccatgaaa tttattggca gcaatcgaca taccgatacc tgtgccacaa acgagaatgc
      781 caaggtcaaa gccatgggat tgaatggctt tggcaacgag ttccgcgtaa cggggataat
      841 cggtgctttc ttcgttgtgc gtgccaaaat cttcatattc aattaagcga ctgtcaaggt
      901 agttttttat ctgttcttta agtaaaaagc ctgcgtggtc tgatgcaatg gcaattttca
      961 ttttttacct ctctttcggg gctaattata ccttctgtat gacaagttgc acagaactag
     1021 tgacaatcat caattgaagt cagtttgtgc tttcattata ttttaatacg taataaagac
     1081 tttaagaagg tgattaaatg atattagtta ctggtggagc tggacatata ggaaacgttt
     1141 tagtacgcga tttggtcgaa aaaggtgaac gcgtacgtgt tttagctttg cctggtgaag
     1201 acgttggctc tcttgatggc ctaaatgttg aaattgtgcg cggaaacatc ttgaatatgg
     1261 agcaactccg tgaagcgatg agtggtgcca ggctggtttt ccatttagca tcgtttgtcg
     1321 ctttgacagc tgacaaatat ccgcttatgc gtcaggtaaa cattaacgga actgcaaatg
     1381 tcatctcttt gtgtaaagag ctgggcgttg aacgcctggt gtataccagt tcaattcacg
     1441 cctttgaacg tgttcctgaa ggtattgtgg ttaatgagga acttagtttt gatacagaaa
     1501 accctgatgg tctttatgac cgtacaaaag ccgaagcaac cttgctggtg cttgacgcgg
     1561 tgaaagaagg actggacgcg gtagttgttt gtccaaccgg cgtggtcgga ccttatgatt
     1621 tcaaactgtc tgaactgggt gaaatgatta ttacctggat gaaaaaacgt gcatctgtct
     1681 caaccgatgg tgcctacgat tttgtggatg tgcgcgatgt ttcgaaaggg catattctgg
     1741 cagctgaaaa aggcctttct ggtgaagtgt atatcctggg cggagaacgt ttgacaatca
     1801 aagctttcag ggcaatggtg caagctgcta attccattaa gacaatggca attaatattc
     1861 ccgccaaact ggctttgttt gtagcaccga ttgctgagtt tttctacaaa ttatttaaaa
     1921 aacggccgaa atttacgacc tatagcatca aaaccttgtt aagtaattcg catattagct
     1981 ataagaaagc tatccatgaa ctgggatatc aaccgcgcca gttaaaagaa actatcaagg
     2041 atacggttga atggctgaag atgaatcaaa gtaaactaca acacggtttg cggatgactt
     2101 cggtgaaacc gacggtataa atctgatttt aatttttttg agaaaataag ctaaattagg
     2161 ggctggcatc tttcacaaaa gtgtgtcccc cgttgtccta tgacagtgcg tgtgatggga
     2221 ttaccgcaac gcgggcaggg attgccggtt tgttggtaga cctggaagta gttttgaaat
     2281 ccaccgcctt tatatgccca gtcgatgctg gctccgttat gttcaatacc aagccgcaaa
     2341 actttacgaa tagcgcgtaa caggtggctt gaatcatcta aactcaagct gttggactgg
     2401 cgtttggggt gcaagccggc ttcaaaaagg gcttcgtcgg tatagatgtt tcccaaacca
     2461 gcgatgaaac tttggtcaag caagagaggc tttagctgac gtttatgttt tcttagctta
     2521 tcagcaaaat tgacatcggt gagtttgggg tcaaagggtt cggggcctag tttagataaa
     2581 atatggttgg ggtcgggcgt tagccaaact ctgccaaact tacgtgtgtc attaaaagcc
     2641 aaatgccagt tatctgcaaa atgaagatag acgtggtcat ggggtacaag gggtttgctc
     2701 agtggcaaag gcatatcggc gtagcgcatt atcaaatcgc cactcatgcg gagatggata
     2761 agaaaaaaga cgtggtctaa gtcaaaaatg agaaacttgc cacgccttga aacgtttagg
     2821 acagattggt tttttacagc tgaatggaaa cttagaaggt ccggttcagc aacagtcttt
     2881 gcccaaaata ccgaactgga gactattctt tgcccaagga ctgaaggtgt ttgttcatca
     2941 cctttagcca gatttcgcgc gatggtctca acttccggca gttcgggcat tattcgttaa
     3001 acatcacacc aacagatgtg ctttggttgg tgcgtttgat gacctcgccc agcagtgggc
     3061 cgatactcaa gaccttgagt cgctcgccaa aagcagcctt ttggttgtcg cttagcggta
     3121 tggtgttggt agtgataaat tgctttactg gcaaggcagt caggcgtttg acggcgttgg
     3181 atgaaagcac aggatgaacg aaagcgacat aaacgtcacg cgcgccattg agtttggctg
     3241 tctcgaccgc ttcgcaaatg gaaccaccgg tgtcgacttc gtcatcaata agaatgacat
     3301 cacggtgctt cacatccccg attaggttca gggcttcggt atggtcgtga ttgtcgatac
     3361 ggcgtttttc aataaaagac agcggaacat caagtcccat agcgtagcgg cgtccatttt
     3421 tggcaaagcc taaatcagcc gtaagaatga cagggttagt gaggtcaggc agcagatcag
     3481 cgagataagg tttgatgatg tgatatgccg aaaggacatc acctgggata gagaaaaagc
     3541 cttgaatttg gccagcgtgc aggtccatgg tgatatagcg atccgcgccg gcaacttcca
     3601 tcatttcaca gactaatttg gctgtgatag gcacacgggg ttggtcttta cggtcagaac
     3661 ggccgtagca catataaggg aagacaagcg tgatgcggcc ggccgagtca agtttgagag
     3721 tttgtgccat gatgagcatt tccatcagat tgcggtgtac cggcattgag gttgtttgaa
     3781 tgatgtaaca gtcctggcca cgaacggagg cgtggagttt tacgaataag ttctcgttgg
     3841 tgaactcgat tacatctcgc ccgcataact caagaccaag ataatcggcg atttcctggg
     3901 caagttccat ggaagccgtg cccgcaaaaa gacgaatatt accgtagcgc atgccaaaat
     3961 ctggctgttg agatgtcatt gttctaacct tccttttgtt tgtttgcgaa ccattcttcc
     4021 tgcaaaatgc tcataagaat gatgtcattg taaaaaccat gccgaaattc agcttttcga
     4081 aagcgccctt cttcaagaaa accagacttg agatagcttt tgcgggcgcg gtgattgctc
     4141 tcaagaacgc gtaaccaaat gcgatgcaaa ttaaggtctt caaagccaaa tttaagcatt
     4201 aagcgcatgg cttcagtgcc ataaccctgg ctccagtatt ccttttcgcc gagaaaaatg
     4261 ccaacttccg cgctgcggtg aatgtcggtg attcccatga aagcacagtt cccgatagcc
     4321 agccattcag atttaccatt tttgctatcc gtaagacgca ggtcaataac aagcggatgt
     4381 tctgccattg gacgtttgag aacggactcg aaccatgctg tttcggcttc aaggctgtag
     4441 ggaaaaatat tgctcaaaaa ttccactacc tcagggtcgt ttatccagcg ttgaaaagtg
     4501 gggatatccg tgcgttcggt tgctcttaat ctgacgtttt caccatagag aatggccatg
     4561 atttgctcca agaatgttaa ttatggttca agacggttgg gtccacggaa gagatagacc
     4621 gcgtcgcgaa tgctgggcag gttgagcatg accatcaaga gacggttaag acctaaacca
     4681 agtccaccgt ggggaggaca accatagcgg aagtagtcaa agtagccttg caaaagttcg
     4741 gtgccgagac ctttttcgat agcctgggct ttaaggactt cgtagcggtg ttcgcgttgt
     4801 gcgccggtgg tgagttccaa gcctttgccg atgaggtcaa agcttttggt aagcaggggt
     4861 tggtcagcgt ggcgcatatg gtaaaacgca cgcaccgtgg caggccaatc ggtaacgaaa
     4921 acaaaatcgt tgccataagt gtttttgacg tattcaccca aagcgcgttc agcgccggga
     4981 tcgagatcac cttttttctc gggtgggagt ttgtaatcca tatctttaag gatggcgtaa
     5041 gcttcagaca tggttaggcg agggaatggt gtttggggta cttcaatttc cacgtcgaaa
     5101 gtttctttaa tttgttcgcc atgcttttct ttgacggcgg tgtacgcgta tgccagcgag
     5161 ttttcgcaaa atgccat
//
LOCUS       DAOSFC010000081         5145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_14379_length_5145_cov_4.162672, whole genome shotgun sequence.
ACCESSION   DAOSFC010000081 DAOSFC010000000
VERSION     DAOSFC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14379_length_5145_cov_4.162672"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PK791_03110"
     CDS             <1..276
                     /locus_tag="PK791_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92335.1"
                     /translation="LIFGKTDGVYEDFPTCQKVLPYINAKSALPGSLTGSSSIDVTGG
                     MLEKVRLMQRLCAVSPETQVRICPANQPGQLMDALLGKDTGTLITHN"
     gene            298..1452
                     /locus_tag="PK791_03115"
     CDS             298..1452
                     /locus_tag="PK791_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOH92336.1"
                     /translation="MIYTRQQLEEFEDQHLAPYAIRSKDSRGRKYPEEEADFRTRYQR
                     DRDRVIHTTAFRRLEYKTQVFIIHEGDYYRTRLTHTLEVAAIGRSLARSLGANEDLTE
                     TICLAHDLGHPPFGHSGEAVLARLMMDHGGFNHNHQSVRIVTELEQRYPDFPGLNLTW
                     EVLEGMIKHETDYDSADASDFDPHLRGHLEAQLANVADELAYTSHDLDDGLRSGLITP
                     AQLDGIAIWEIVNESLGRRKNDPLDELSRHQVIRRLIGFEMTDLIHSIDRYLRRSKAS
                     TPQELQALPYNVVGFSEDMHRRNRELKDFLFEKLYRHYRVVRMQTKAERIITDLFELY
                     TANPGALPDTTQLLIPSKGLELTVCDYIAGMTDRFAIDEHKRLFDVDVLP"
     gene            1457..1927
                     /gene="greA"
                     /locus_tag="PK791_03120"
     CDS             1457..1927
                     /gene="greA"
                     /locus_tag="PK791_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOH92337.1"
                     /translation="MNKTPYLTEAGKRKIEEELADLRGPQRDEIAARLKSAIEMGDLS
                     ENADYHAAKEAQGFLEGRILELETLLFRAEIVDESEFESNVVNIGSKVTVQEAGEDEE
                     VYIIVGAKEANLRERKISYESPMGASLLGKKLGETVQVPLPNGNELSLKIIAIE"
     gene            complement(2016..3221)
                     /locus_tag="PK791_03125"
     CDS             complement(2016..3221)
                     /locus_tag="PK791_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92338.1"
                     /translation="MENFLSEFKEKLKVPRNLAIFTGVIGLILGLILAWGVFPVKWKD
                     ASAEHLRPDERVVYLRNAINSYTMTGRVKEAQMAWDSLGSNAVKTLEDLNQEPGFLYT
                     EQIQRFASAVDTSAPAPVTPPEDNNAPVNENGQVIGLRPTDEIPEPESKSKGLLWLGI
                     ILLLLLLVGAGLIWWFFFKNKDFKLPEKLKLRRKDTSTPQYAAVKPAATPIKREPKPQ
                     PVVQEPVEEPEEKPTLPNLADLFGLNDRQKAQEEVPEPLPAAKPVAQFMTTYMLGDDM
                     YDESFTFDAPNGEFLGECGVSSSDTIGVGEPKKVSAFDIWLFDKNDIQTVTKVVMSKH
                     AFKDAGTRQRLEIRGEPIMAEDGKQFVLETATLRLEAKLVNFQYGDLPLPDDSYFQRA
                     TMELAIYRK"
     gene            complement(3352..>5145)
                     /gene="topA"
                     /locus_tag="PK791_03130"
     CDS             complement(3352..>5145)
                     /gene="topA"
                     /locus_tag="PK791_03130"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOH92339.1"
                     /translation="KRVVFHEITKSAIDAAFKNARTLDMDLVDAQQARRILDRLVGYK
                     VSPILWRKVRSRLSAGRVQSVAVRLVVEREREIQNFVPVEYWVVSAIFHQFGHTEQYE
                     AKLLKINDQDLELNNEKDTLATVADMKEAKYKLAEKKLNSRRNRPVAPYITSTMQQDA
                     SSRLGFLASRTMMVAQQLYEGIKLSGSEETGLITYMRTDSVNVSAQAIAEVRDYIAKE
                     YGESYLPEKAQVYKTRAKTAQEAHEAIRPTSVHRTPEKMRVFLTPEQHRLYTLIWQRF
                     VASQMEAQLVETLSLDITGQSQAHKYLLRATASQTVFEGFRKVYPTANPENNENPELV
                     KLFKDVLKEGAEQILDDLNYEQKFTQPPARFSEASLILMLEENDIGRPSTYAPIISTI
                     QTRGYVERQGRTLKPTEVGFIVNDLLVEFFPEIVDIKFTSRMEDELDEIAEGKADWVK
                     VLQEFYTPFEKRLDYAAENMPNRKPAPKEIGRACPDCGEPLVMRSGRFGDFISCSTFP
                     ACRYTENIPVTLEMACPVCQDGDVVQKRTKRGKTFYGCSRYPECEFASWQKPIQEPCP
                     SCGGLLTEKSNDEAVCTECKLTFPQIDGKLV"
BASE COUNT         1306 a         1356 c         1208 g         1275 t
ORIGIN      
        1 ctcatctttg gcaaaaccga tggggtctac gaagactttc ctacctgcca aaaagttctg
       61 ccctacatca acgccaaaag cgccttaccg ggcagtttga ccggttcaag cagcatcgac
      121 gtgaccggcg gcatgcttga gaaagtccgc ctcatgcaaa ggctatgcgc agtcagccct
      181 gaaacccagg ttcgcatctg tccagccaac cagccgggtc aattaatgga cgctttactc
      241 ggaaaagaca cgggaacact tatcacccat aattaatcac agttcaaagg atatgccatg
      301 atatacacac gccaacaact tgaagaattt gaagaccagc accttgcgcc ttacgccatc
      361 cgcagcaaag actcacgcgg acgaaaatat ccggaagaag aagctgattt ccgcacccgc
      421 tatcagcgcg accgcgaccg ggttatccac accaccgctt tcaggcgttt ggagtacaaa
      481 actcaggtct ttatcatcca cgaaggcgat tactaccgca cccgcctgac ccacacgctg
      541 gaagtggctg ccatcgggcg cagtctcgcc cgttcacttg gcgcgaacga agacctgacc
      601 gaaaccatct gcctggcgca cgacctgggg cacccgccct ttggccattc aggcgaagcg
      661 gttttggcgc gcttgatgat ggaccatggt ggcttcaacc ataaccacca aagtgtgcgt
      721 atcgtcaccg aacttgaaca gcgctatcct gattttcccg gtctgaacct gacctgggaa
      781 gtgctcgaag gcatgataaa acacgaaacc gactacgact ccgccgacgc ttcggacttt
      841 gacccgcatc tgcgcgggca tctggaagcg caactggcga acgtcgccga cgaactggct
      901 tatacctccc atgacctgga cgatggcttg cgctccgggc tgatcacgcc cgcccagctg
      961 gacggtatcg ccatttggga aatcgtcaac gaaagccttg gacgccgcaa aaatgacccc
     1021 ttagacgaac tgagtcgcca tcaggtcatc agacgtctga taggctttga aatgaccgat
     1081 ttaatccaca gcattgaccg ctatttgcga cgttctaagg ccagcacacc ccaggaactg
     1141 caggctttgc cctataacgt ggtgggattt agcgaggata tgcaccgacg caaccgcgaa
     1201 ctgaaagatt tcctgttcga aaagctttat cgccattatc gcgtggtgcg catgcaaacc
     1261 aaggcggaac gcattattac cgacctgttt gaactctaca ctgccaaccc cggtgcttta
     1321 cctgatacca cgcagctcct tatccccagc aaagggcttg agttgaccgt ttgcgattat
     1381 atcgccggca tgaccgaccg tttcgcgatt gatgaacata agcgtctttt tgacgtggat
     1441 gtgttaccat agagccatga acaagacacc ttacttaacc gaagctggaa agcgaaagat
     1501 tgaagaagaa ctggctgacc tgcgtggtcc gcaacgcgat gaaattgccg cccgccttaa
     1561 aagcgccatc gaaatgggcg atttgagtga aaacgccgat tatcacgccg ccaaagaagc
     1621 ccagggcttt cttgaagggc gtattttgga actggaaaca ctgcttttcc gcgccgaaat
     1681 cgttgacgaa agcgagtttg aatcgaatgt cgtcaacatc ggcagcaaag tgaccgtgca
     1741 ggaagcaggc gaagatgaag aagtctacat catcgtcggt gccaaagaag ctaacttgcg
     1801 cgaacgtaaa atctcttacg aatccccgat gggcgccagc ttgctcggca aaaagctcgg
     1861 agaaaccgtt caggtgcctt tacccaacgg caacgaactc agtctgaaaa tcatcgcgat
     1921 tgaataaaac ttcgtcttta gaaaattcat tagaaaagca aaagagacta tcgtttgtgg
     1981 atagtctctt ttttattgat tagtttatgc gtgctttatt tgcgatagat ggccagttcc
     2041 atcgtcgcgc gctggaagta gctgtcatcc ggcagcggca ggtcgccata ctggaagttg
     2101 accagcttgg cttccaaacg cagagtggcc gtttccagca caaattgctt gccatcttcc
     2161 gccatgatag gctcgccacg gatttcaagg cgctgacgtg taccagcatc tttgaaagcg
     2221 tgtttgctca tgaccacttt cgtcaccgtt tgaatgtcgt ttttgtcaaa cagccaaatg
     2281 tcaaaagcgg aaactttttt gggttcgcca acaccgatgg tatccgaact gctcacacca
     2341 cattcgccca aaaactcacc gttgggtgca tcaaaggtga atgattcatc gtacatgtcg
     2401 tcaccaagca tgtaggtggt catgaactga gcaaccggtt tcgcggctgg caaaggttcg
     2461 ggtacttctt cctgcgcttt ttgtcggtcg tttagcccga acaaatcagc caggttcggc
     2521 aaggtcggtt tctcttccgg ttcttcaact ggttcctgaa caacgggctg tggctttggt
     2581 tctcgtttaa tcggagtcgc ggctggcttt acagcagcgt attgtggggt tgaagtatcc
     2641 ttacgacgta acttcagttt ttcaggcaat ttaaagtctt tgtttttgaa gaagaaccac
     2701 caaatcaagc ccgcgccaac caacaatagt aaaagaagaa tgatgcccag ccaaagcaag
     2761 cctttgctct tgctttcagg ctccgggatt tcatccgtcg gacgcaatcc aatgacctgg
     2821 ccattttcgt ttacaggcgc gttattgtcc tctggcggtg taacaggtgc cggcgcggaa
     2881 gtatccaccg cggaagcaaa gcgctgaatt tgttcagtat acaaaaagcc aggctcttga
     2941 ttgaggtctt caagcgtttt aaccgcgttt gatccaagtg aatcccaggc catttgcgct
     3001 tctttcactc ggccagtcat ggtataggag ttgatagcat tacgcaggta aaccacccgc
     3061 tcgtcagggc gcagatgctc cgcgctggcg tctttccact tgaccggaaa tacaccccac
     3121 gccaatatca agcccagaat taagccaata acacccgtaa agatggctag attacggggc
     3181 acttttagtt tttctttaaa ttccgaaagg aagttttcca tttggcacct cattcataca
     3241 taacgttcaa cgttataggt gcacgtttta taccagcaga aattaaaaaa cgctccgaac
     3301 gagaaaattc ggagcgttta tatataacaa attagccttt aaaaaggcgc cttaaacaag
     3361 ctttccatca atttgcggaa aagtaagttt acattcagtg caaaccgctt catcattcga
     3421 tttttcggtc agcaaaccgc cacagctggg acatggctct tgaatcggct tttgccatga
     3481 cgcgaattcg cattccggat agcggctaca gccataaaaa gtttttccgc gtttggtgcg
     3541 cttttgtacg acatcgccat cctgacaaac cggacaagcc atttcaagcg tcacagggat
     3601 attttccgta tagcgacaag ccggaaaagt gctgcaactg ataaaatcgc caaaacgccc
     3661 cgaacgcatc accaggggct caccgcaatc agggcaagca cgtccaattt ctttgggagc
     3721 aggttttctg ttgggcatat tttctgcagc gtaatccaat cgcttttcaa aaggcgtata
     3781 aaattcctgc aaaactttaa cccagtctgc ttttccttca gcgatttcat caagttcgtc
     3841 ttccattcgg gaagtgaact tgatgtccac aatttcaggg aaaaattcaa ccaataaatc
     3901 gttgacgatg aagccaactt cggtcggttt gagtgtgcga ccctggcgtt ccacataacc
     3961 ccgcgtttga atggtcgaga taatcggcgc gtaagtgctt gggcgaccga tatcattctc
     4021 ttccagcatt aaaatcagcg aggcttccga aaaacgcgcc ggaggctggg tgaatttctg
     4081 ctcatagttg agatcatcca gtatttgttc cgcgccttct ttcaaaacat ccttaaaaag
     4141 tttaactaat tcagggttct cgttattttc agggttagcg gtgggataaa ccttgcggaa
     4201 cccttcaaac acggtttggc tggcggtcgc gcgcaacaaa tatttatgcg cctgcgattg
     4261 ccctgttata tccagactaa gcgtttcaac aagctgggct tccatctgac tggcgacaaa
     4321 gcgttgccaa atcaaggtgt aaagccgatg ctgttccggg gtcagaaaga ctcgcatttt
     4381 ctctggcgtc cgatgcaccg aagtcggacg aatggcttcg tgcgcttcct gcgcggtttt
     4441 agcgcgggtc ttgtaaacct gcgctttttc tggcaagtag ctttcgccat actctttggc
     4501 aatatagtca cggacttcag cgatagcctg agcggacaca ttcaccgagt cggtacgcat
     4561 ataggtaatt aaacccgttt cttctgatcc gcttagcttg atgccttcat aaagttgctg
     4621 tgccaccatc attgtgcgac tggccaaaaa gcccaggcga ctggatgcgt cctgctgcat
     4681 ggtactggta atatacggcg cgacaggtcg attacggcgc gagtttagct ttttctcagc
     4741 cagcttatat ttggcttcct tcatgtccgc aacagtcgct aaggtgtctt tttcgttgtt
     4801 tagctccaaa tcctggtcgt tgatcttcag cagtttggct tcatactgtt cagtgtgtcc
     4861 aaattgatga aatatcgctg acacaaccca atactcaacc ggcacaaaat tttgaatttc
     4921 acgctcgcgc tcaaccacca ggcgcaccgc caccgattga acacgtcccg cggaaagacg
     4981 actgcgcact tttcgccaca agatagggct gactttataa ccaaccagac ggtctaatat
     5041 acgccttgcc tgttgcgcgt caaccaaatc catatccagc gttcgcgcgt ttttaaaagc
     5101 tgcgtcaata gctgacttgg taatttcgtg aaaaacaacg cgttt
//
LOCUS       DAOSFC010000082         5073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_14667_length_5073_cov_3.911120, whole genome shotgun sequence.
ACCESSION   DAOSFC010000082 DAOSFC010000000
VERSION     DAOSFC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14667_length_5073_cov_3.911120"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..>2102)
                     /locus_tag="PK791_03135"
     CDS             complement(228..>2102)
                     /locus_tag="PK791_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOH92340.1"
                     /translation="HYKEMQDVEFTVEDGKLWMLQTRNGKRTAKAAVKIAVDMVKEGL
                     IDKVEAVKRVTPEQVDNLLHPQFSHEAIEQAHKDGRYFAKGVNASPGAAVGRIYFDPD
                     TVQRKHQDEGQDVIMVRPFTKPDDVHGMLSAKGILTSEGGATSHAAVVARQFGVPCVV
                     GASAIAIDAEKLQMVANGKTVKEGEWISVDGTTGEVYVGQLELSQVDITEQKELMQLL
                     SWADEISRLQVWANADYPADATRAREYGAKGIGLCRTEHMFFDVERLPDVQMMIMNAE
                     ETQRMLNSIEVLQHAYDTGKLEGRTLNEEEMAELKAEIEQETAKMRATPAAQAYFKAL
                     EIILPQQRGDFYGLFEAMDGLPVIIRLIDPPLHEFLPSMEELLVEVATMRAKGETEGL
                     AEKENLLAVVQGLHESNPMMGLRGIRLGIVYPDIMKMQVRAIFEAACDAKLNGIDPHP
                     EIMIPLTGIVTELEQVQPTLVQIAKDVMAEKGVQVDYQFGTMIEIPRACITAEEIAKY
                     AEFFSFGTNDLTQMSFGYSRDDAERTFLAEYVEKGILPKNPFQTLDRKGVGALMKLAV
                     EKGRSVRPDLTVGICGEHGGDPSSIEWCHLIGNNYVSCSPFRVPLARLATAHAQLKHG
                     "
     assembly_gap    2103..2202
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2203..3226)
                     /locus_tag="PK791_03140"
     CDS             complement(<2203..3226)
                     /locus_tag="PK791_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOH92341.1"
                     /translation="MTDKKWVYLFSEVDQAEAYAGTWDGVRGLLGGKGANLGDMIRAG
                     VPVPPGFTITTEACNAFQKTREFPEGMWDQVLAAVKALEEETGKKFGDPVNPMLVSVR
                     SGSKFSMPGMMDTVLNLGLNEETAEGMIKITGNPGFVDDSYRRLVHMLGTVVLGIDDE
                     YFEEPLEAYKAEKGYKLDIEMTADDWAEIIKTYLAVVKEHKGFDFPMDPYKQLEYAVK
                     AVFDSWDSRRAIDYRRRENIPDDLGTAVNVQLMVFGNSGNNSGTGVAFTRNPQNGENE
                     MYGEFLVNAQGEDVVAGIRNADPIAEMAQIWPEVYQQFFDITERLEHHYKEMQDVEFT
                     VEDGKLW"
     gene            complement(3434..3976)
                     /locus_tag="PK791_03145"
     CDS             complement(3434..3976)
                     /locus_tag="PK791_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH92342.1"
                     /translation="MMKNEAIIDAIYARRSIRKFTDEAVSDEHIDILLKAAMAAPSAT
                     NAQPWRFVVTRDPVRLAAIRKEMPAGKINAPLAILVCADLTIFKRPITERFWTQDCSA
                     ATQNILLAAVGLGLGTVWCGVHPVPLIEKRMEKLLDLPANVKVLNVIYIGHPAEEKEA
                     RTQYDANKVFKDRYGEPWQD"
     gene            complement(3978..>5073)
                     /locus_tag="PK791_03150"
     CDS             complement(3978..>5073)
                     /locus_tag="PK791_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92343.1"
                     /translation="AMAIDVDQADVLIDPLGGEADLKQGVLREASPDAFMNDPLRVMR
                     AVCLAQAFNLGISFKTLEHLRVATTELRRVSGERIRDEFFKILDEKDYEASLRQMWEL
                     GILSEIFSCLPCYSQDGVSVSNQKVEKRLFRLKCLEQLYQDLESEQGLVSTSASEKQR
                     QAHIAGWQEQLNAYLDMQITPGRTVRNHLVLVALLLDLERENASESPIQLVANSFKLS
                     KPESDFLSRIEKAFLSGVFSVSCERHLIDMEVYRLLKELKIASPAYALLGLVLADTEL
                     EHASKALNICQENSLNLLSVWFQRGGEILKPNLLMDGNEIMHYAGLAPGPIIGDLLEK
                     LEEAQFLGLIETIDQAKAFIDKELVQNNKR"
BASE COUNT         1237 a         1311 c         1104 g         1321 t
ORIGIN      
        1 ggcaggaaat gagcaaagac gccgactacg acctcttttt aagcaccatt cgcttcctgg
       61 aaactcgtat ctacgtccgc cgcatcgtcg aaattcacaa tctctatcgt ttgatttata
      121 gtaattaacc aactcatcag aataagaaac aaacattaaa tgtaaaacaa aaaaggcgag
      181 ttgtttctaa ctcgcctttt tgttttactt aagcgttttt agccagatta gccgtgtttc
      241 aactgcgcgt gggcagtggc caggcgggcc aagggcacac ggaagggtga acagctaacg
      301 tagttgttgc caatcaggtg acaccattcg attgaactcg ggtcaccacc atgctcgcca
      361 cagataccca cagtcaggtc aggtcgcaca gagcgtccct tttcaacagc cagcttcatc
      421 agcgcgccaa cgcccttgcg atccagggtc tggaaggggt tcttgggcag aatacctttt
      481 tcaacatatt cagccaggaa ggtgcgctcg gcgtcatcgc ggctgtaacc aaaggacatt
      541 tgggtcaggt cgttggtacc gaatgagaag aattcagcgt atttcgcgat ttcttctgcg
      601 gtaatacaag cgcgggggat ttcaatcatc gtaccaaact gataatcaac ctgaacaccc
      661 ttttctgcca tcacgtcttt tgcgatttgg accagggtcg gctggacctg ttccaactcg
      721 gtgacgatac cggtcagggg gatcatgatt tcaggatggg ggtcaatgcc gttcagtttg
      781 gcatcgcaag cagcttcaaa gatagcgcga acctgcatct tcatgatgtc ggggtaaacg
      841 atacccaggc ggataccgcg cagacccatc atggggttgc tttcgtgcag gccttgcacc
      901 acagccagga ggttttcttt ctcggccagg ccttcggttt cgcccttggc gcgcatcgtg
      961 gcaacttcaa ccaaaagctc ttccattgaa ggcaggaact catgcaatgg tgggtcaatc
     1021 aggcgaatga taacgggcag gccgtccatc gcttcaaaga gaccgtaaaa gtcaccgcgt
     1081 tgctggggca agatgatttc gagtgccttg aagtaagctt gcgcggctgg tgtagcacgc
     1141 atcttggcag tttcttgttc aatttcagcc ttgagttcag ccatttcctc ttcattcaaa
     1201 gtgcggcctt caagcttgcc agtgtcatag gcatgctgta aaacttcaat gctgttcagc
     1261 atacgctggg tttcttcagc gttcataatc atcatttgca cgtcgggcaa gcgctcaacg
     1321 tcaaagaaca tatgctcggt gcggcaaagg ccgataccct tagcgccgta ttcgcgggcg
     1381 cgggtagcgt cagcgggata gtccgcgtta gcccaaacct gcaagcggga gatttcatca
     1441 gcccagctca aaagttgcat cagttctttt tgttcagtga tatcaacctg gctcaattcc
     1501 agttgaccca cgtaaacttc accggttgta ccgtcaacag aaatccattc gccttctttg
     1561 acagtcttgc cattcgcgac catttgcagt ttttccgcgt cgatggcaat cgctgacgcg
     1621 ccaaccacac atggcacacc aaactggcgg gcgaccactg cggcgtgaga tgtcgcgcca
     1681 ccttcggagg tcaaaatacc cttggcggaa agcataccat gcacatcatc gggtttggtg
     1741 aaaggacgta ccatgataac atcctgacct tcgtcctgat gttttctttg cactgtgtcg
     1801 gggtcaaaat agatgcgacc gaccgcggca ccaggtgacg cgttcacacc cttagcaaag
     1861 taacggccat ctttgtgggc ctgttcaatc gcttcgtgtg agaactgggg atgcagcaag
     1921 ttgtcaacct gttcgggagt aacacgcttg acagcttcaa ctttgtcaat caggccttcc
     1981 ttgaccatat ccacagcaat cttcacagct gcttttgcgg tgcgtttgcc gttgcgggtc
     2041 tgtaacatcc acaatttacc atcttcaacg gtaaattcaa catcttgcat ttctttgtaa
     2101 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntccacaat ttaccatctt
     2221 caacggtaaa ttcaacatct tgcatttctt tgtaatggtg ctcaaggcgt tcggtaatat
     2281 caaagaattg ctgataaacc tcaggccaga tttgtgccat ttcagcgatg gggtcagcat
     2341 tgcggatacc tgccacaacg tcttcaccct gggcgttcac caaaaactcg ccgtacattt
     2401 cgttttcgcc gttttgcggg ttgcgggtaa aagcaacacc ggtacccgag ttgttgcccg
     2461 agttaccgaa gaccatcaac tgcacgttca ctgcggtgcc caggtcatcc ggaatattct
     2521 cacgtcggcg atagtcaatc gcgcggcggg aatcccagga gtcaaacaca gcctttacgg
     2581 catattccaa ctgtttgtag ggatccatcg ggaaatcaaa gcccttgtgc tcttttacaa
     2641 cagccaggta agtcttgata atttcagccc agtcatcagc ggtcatctcg atatcgagtt
     2701 tgtagccttt ttcggctttg taagcttcca ggggttcttc gaaatactcg tcgtcgattc
     2761 ccaaaaccac cgtgcccaac atgtgcacca agcggcgata ggagtcgtcc acaaaaccgg
     2821 gattgcctgt gattttaatc ataccttcgg cagtttcttc gttcaaacca aggttcaaaa
     2881 cggtgtccat catgccgggc atcgagaatt tcgaacctga acgaacggat accaacatcg
     2941 gattaacggg atcaccaaat ttctttccgg tttcttcttc cagtgccttg acagcagcca
     3001 aaacctgatc ccacattcct tctgggaatt cacgggtttt ctgaaaagcg ttacaagcct
     3061 cagtagtgat tgtaaagcca gggggtaccg gaacaccggc gcggatcata tcacccaggt
     3121 tggcaccctt gccacctaat aaaccgcgca caccatccca ggtgccagcg taagcttcgg
     3181 cttggtcaac ctcgctgaat aaataaaccc attttttatc tgtcattgtt cctccataaa
     3241 gttttatcga aacgcgcgaa cgtttcagtt gttttcgcaa ctatgaatga taccacgtat
     3301 agcccttgtt tacctgcaaa gtttctactt aaatgcttga attaacttag attcttatac
     3361 tatatttaca cactcaaaac gggaaccccg cattttattg aaatatgaaa tatcttataa
     3421 gacttggcgt gttttaatcc tgccagggct cgccataacg gtccttaaaa accttgttag
     3481 cgtcatattg cgtacgtgct tctttctctt cagctgggtg tccaatgtaa ataacattaa
     3541 gcactttgac gttcgccggc aagtccaaga gtttctccat gcgtttttcg atgagtggca
     3601 ccggatgcac cccacaccac accgtaccca gccccagacc taccgcggct agcaaaatgt
     3661 tctgcgttgc cgctgagcag tcttgcgtcc aaaaccgttc ggtaatcggt cgtttgaaaa
     3721 tcgtgagatc cgcgcaaact aaaatggcca gcggcgcatt gattttccct gcgggcattt
     3781 ccttgcgaat tgcagccagt cgaacagggt cacgtgtgac cacgaaacgc cagggctggg
     3841 cgttcgtcgc gctgggtgcg gccatagccg ctttcaaaag gatgtcaatg tgctcatcgc
     3901 tgacagcttc gtccgtaaat ttccggatgc ttctgcgggc ataaatagca tctataatcg
     3961 cttcattctt catcatttca cctcttattg ttttgtacca gttccttgtc gataaacgct
     4021 ttcgcctggt cgattgtttc gataagtccc aaaaactggg cttcttcgag tttttccagc
     4081 aagtcgccga tgattggtcc tggtgccaag ccagcgtaat gcataatctc gttgccatcc
     4141 atcagcaagt ttggtttcaa tatctcccca ccacgctgaa accagactga aagcaaattc
     4201 aaactgtttt cctggcaaat atttaatgcc tttgatgcgt gttctaattc agtatcagct
     4261 aaaaccaagc caagtaacgc gtaagctggt gatgcaattt tgagttcttt aagcagccta
     4321 tagacttcca tatcaattaa atgacgttca caggaaactg aaaaaacacc ggacaaaaat
     4381 gctttctcaa tcctgctcaa aaaatcactt tccggcttgc ttaacttgaa agaatttgct
     4441 acaagctgga taggactttc cgacgcattt tcacgctcta aatccagcaa aagtgccact
     4501 aaaaccaagt gattgcggac tgtcctgcca ggcgtgattt gcatatcaag atacgcgttt
     4561 agctgctctt gccagcccgc gatatgtgct tgcctttgct tttcagacgc ggaagtacta
     4621 actaaccctt gctcactttc caggtcttgg tacaactgtt ccagacactt aagccggaac
     4681 aatcgctttt caactttttg atttgaaacg cttaccccat cctgagaata acaaggcagg
     4741 caagaaaaaa tttcactcag tatccccagt tcccacatct gccttagtga ggcttcgtaa
     4801 tccttttcat caaggatttt aaagaattca tcacgtatgc gctcacctga aacacgcctc
     4861 aattctgttg tggctacgcg cagatgctct aaagttttaa aggatatccc cagattaaac
     4921 gcctgcgcca agcacactgc acgcatcaca cgcaaagggt cattcataaa agcatcaggt
     4981 gatgcctccc gcaatacccc ttgtttgagg tcggcttcac cacccaatgg gtcaatcaaa
     5041 acatccgctt gatccacatc aattgccatt gcg
//
LOCUS       DAOSFC010000083         5061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_14716_length_5061_cov_5.586696, whole genome shotgun sequence.
ACCESSION   DAOSFC010000083 DAOSFC010000000
VERSION     DAOSFC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14716_length_5061_cov_5.586696"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1667)
                     /locus_tag="PK791_03155"
     CDS             complement(48..1667)
                     /locus_tag="PK791_03155"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92344.1"
                     /translation="MLKEGLFKRVRNKLALSLDEIDNKARIRKLAEIINENKKPAQGQ
                     AVIFFNASTRLSRLSLNAGFSRLASWALELQGVPVVHFVCQRGLQPCVLGTNRKDERL
                     RTPCHTCILESEILFEDSQSINFDFSEDHVLESVLEGLDLEDLYDFTHLGIPLGALVL
                     PAVRWILRRHHLEDDEMTLMIYKGYVRSAWSFTQHFEKALDELAPRALVVYNGVLYPE
                     GVAKWLAQRRGIRVVSHEVGFQPLSAYFTDGDATAYDLDIPADFALDESQNERLDNYL
                     RKRFQGDFFMAGVQFWPEMSQLSPAFLAKAREFRQVVPVFTNVIFDTSQVHANLIFEH
                     MFAWLDNLLEVAKANPDTLFVVRAHPDEAREGKASEESVADWAKSRDVENLPNVLFIP
                     PHEFINSYDLIRMAKFVMIYNSTIGMEASILGATVLSAGKARFTASNVVHFPADRKAY
                     DQTLQDFLNAESLSALPEHKINARRFLYYQLYRSSLVFDAFLEQDKYWQGYVMLKDFD
                     WQSLLPENSPAVQVFLDGMLANGNFILKENA"
     gene            complement(1671..2543)
                     /locus_tag="PK791_03160"
     CDS             complement(1671..2543)
                     /locus_tag="PK791_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOH92345.1"
                     /translation="MTREVLIDNTKIGDGHPTYIIGEIGINHNGDVEIAKQLMLAAKQ
                     GGMNAVKFQKRTPEICVPLHQREQMRDTPWGYIPYLEYRYKTEFGKDEYTEIDRYAKE
                     IGITWFASEWDIPSVEFIEGFNPVAHKIPSALITDKELLRAYKSTGKPIIVSTGMSTM
                     EEIQTAIDILGLDDLIICHTTSSYPCPPEELNLRMIPKLKSLYDCPIGYSGHEVGLVT
                     TAVAVAMGACLVERHITLDRAMWGSDQSASVEPQGITRLVKYIRVTERSLGDGIKRVY
                     DSEQSSLSKLRRYS"
     gene            complement(2556..3617)
                     /locus_tag="PK791_03165"
     CDS             complement(2556..3617)
                     /locus_tag="PK791_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOH92346.1"
                     /translation="MIDIEFYMKVYDLKKRLMNGEKMPRAFLEEQFEAFRSPDPVVYN
                     IETTNACNMRCEMCPRTTMMTRPIETMSPELFKRVIDQLKPFSTEQLNRWEKFVTENY
                     GIHKNDMSENHFFLYVIPRVIVLHGYGDPLLDKNMPQYVKWMTERGLESYFSCNPANI
                     NMERTIETFENGLGYVKYSIESVDDDRHKEVRGKASNFTESYKNILKLLELKAQRNYQ
                     TTIVITMINLNKPWQADEFERLKEAFKGLDVYVYLKSQDQMWYEDTQQVTQSIHWLEF
                     CQFPWSSMTIKSNGEATECVEDFNNEIILGDTRTESLYDIWNGAKYIQFRKDHFDLKP
                     GIKCTEQCDMNLIGQFLSA"
     gene            complement(3620..4882)
                     /locus_tag="PK791_03170"
     CDS             complement(3620..4882)
                     /locus_tag="PK791_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HOH92347.1"
                     /translation="MVEKQEVLAIIPARGGSKGIPGKNIKPFAGYPLIAYSILAALRS
                     EHVSRVVVSTDDEEIARISREWGAEVPFIRPAELAQDHSTDYPVIVHALEWLAEHENY
                     HPDVVVQLRPTSPVRPTRLVDDAIDILLAHPEADSVRGVVPSAQNPFKMWTIDPDTGE
                     MKTLLTVPGIKEPYNAPRQALPDTFWQTGHIDAIRVASVFEKESLSGDVIYPLYIDPK
                     FTVDIDIPSDWPVAEATLRELAAEVVDPANLRRAFPARPRFLVMDFDGVFTDNRVIVD
                     ENGLESVRADRSDGLGFGLLKAQTPVECMIISKERNLVVAQRAKKLGIPVLQAIDDKA
                     TALRDEMRKRGMRAEETIFVGNDVNDLPTLDVAGYFVAPADSHPEVIRRADLILTKKG
                     GRGALRELCDMLIRHWQTISHHETLNGD"
     gene            complement(4876..>5061)
                     /locus_tag="PK791_03175"
     CDS             complement(4876..>5061)
                     /locus_tag="PK791_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH92348.1"
                     /translation="SPGGIFNGHNDDFSRNYATKTIMGRMADIDEMDAAMLFLCSDAS
                     SYMTGSNLVVDGGWTAW"
BASE COUNT         1252 a         1242 c         1254 g         1313 t
ORIGIN      
        1 cgaatcgtgg cgcatgggca ccagggcaac aattttatca agactcatca ggcgttctcc
       61 tttaatataa aattgccgtt ggcaagcatt ccgtccaaaa agacctgaac ggcgggggaa
      121 ttttcgggta acaagctttg ccagtcaaaa tctttgagca tcacatagcc ttgccagtat
      181 ttatcctgct ccaaaaaggc atcaaagacc agggatgaac gataaagctg gtaatacaaa
      241 aagcgtcgcg cgttgatttt atgttccggg agtgctgaaa ggctttccgc gttcaggaaa
      301 tcctgcaggg tttggtcata agccttgcgg tcagctggga agtgcactac attggacgcg
      361 gtaaagcgcg ctttgccagc agaaagcacc gtcgcgccca aaatggacgc ttccatgccg
      421 atggttgagt tgtaaatcat cacaaatttg gccatgcgga taaggtcata ggaattgatg
      481 aactcatgcg gcggaatgaa gagaacattt ggcaaatttt caacatcgcg cgattttgcc
      541 caatccgcga ccgattcctc gctggcttta ccctcgcggg cttcatcagg atgggcgcgc
      601 accacaaaga gtgtgtcggg attagctttg gcgacctcaa gcaggttgtc aagccaggca
      661 aacatatgct caaaaatgag attagcatgt acctgactgg tgtcaaaaat cacgttggtg
      721 aacacaggta caacttgcct gaactcacgc gcttttgcca aaaacgcagg actcagctgg
      781 ctcatctcgg gccaaaactg cacgccggcc ataaagaagt cgccctgaaa gcgtttgcgc
      841 aggtagttat ccaggcgttc attttgggat tcatcaagcg caaagtctgc tggaatgtct
      901 aaatcgtaag ctgtggcgtc accatcggta aagtaggcac tcaagggttg aaagcccact
      961 tcatgactca caacgcgaat gccccgccgt tgcgccagcc atttcgcgac accttcgggg
     1021 tacaaaacgc cgttgtaaac gaccagggcg cgtggcgcga gttcgtccaa ggctttctcg
     1081 aagtgttggg tgaatgacca cgctgaacga acatagccct tgtaaatcat caaggtcatt
     1141 tcgtcgtctt ccaggtgatg gcggcgcaag atccagcgca cggctggcaa aaccaaggcc
     1201 cccaaaggaa tgcccaggtg tgtgaaatca tataagtctt caagatcaag gccttcaagc
     1261 acgctttcca gcacatggtc ttcgctaaaa tcaaagttta tgctttgcga gtcttcaaac
     1321 aaaatttctg actccaggat gcaggtatgg cagggcgtgc gcagtctttc atccttgcga
     1381 tttgtgccca gaacgcatgg ctgcaaacca cgttgacaca caaaatgcac cacgggcaca
     1441 ccttgcagtt ccagcgccca ggatgccagg cgcgaaaaac ccgcgttgag actcaagcgc
     1501 gaaaggcgcg ttgacgcatt gaaaaaaatg accgcttgcc cttgggcagg ctttttattt
     1561 tcgttgataa tctcagcaag cttgcgaatg cgcgctttgt tatcaatttc gtccagactg
     1621 agcgcaagtt tattgcgaac tcttttaaac aatccttctt ttagcatagc ttatgaatag
     1681 cgccttaatt tgctcagtga ggattgctcg ctgtcataaa cccgcttgat gccatcgcca
     1741 agcgaacgtt cggttacgcg gatatatttc accagacgtg taatgccctg gggttcaact
     1801 gacgcggact ggtcagaccc ccacatagcg cggtcaagcg tgatgtggcg ttccaccagg
     1861 caggcaccca tcgccaccgc cacggcagtg gttaccaggc caacttcgtg accactgtaa
     1921 ccgatggggc aatcataaag cgatttcagc ttgggaatca tgcgcaggtt gagttcttca
     1981 ggcgggcagg gataactgct ggtggtatgg caaatgatca aatcatccag tccaaggata
     2041 tcaattgcag tttgaatctc ttccatggta gacatgcccg tggagacgat tatcggcttg
     2101 ccggttgatt tataggcacg caaaagctct ttgtcggtta tgagcgcgct cggaatttta
     2161 tgcgcgacgg gattgaaacc ttcgataaac tcaaccgatg ggatatccca ctccgacgcg
     2221 aaccaggtga tgccaatttc tttggcatag cggtcaatct cggtgtattc atctttgcca
     2281 aattccgtct tatagcggta ttccaggtaa ggaatgtagc cccagggagt atcgcgcatt
     2341 tgttcgcgct gatgcaaagg cacacagatt tcaggggtgc gtttttggaa ctttaccgcg
     2401 ttcatgccac cttgtttggc agccagcatg agttgtttgg caatttcgac atcgccgttg
     2461 tggttgatgc ctatctcccc aatgatgtag gtggggtgac catcaccgat ttttgtgttg
     2521 tcaattaaaa cttctctggt catcaatact ccttatcagg cactcaggaa ttgtccaatt
     2581 aagttcatgt cacattgttc ggtacattta atgcctggtt tgagatcaaa atgatctttg
     2641 cggaattgaa tgtacttcgc gccattccag atatcataca agctttcggt gcgcgtatcg
     2701 ccaaggatga tttcattgtt gaaatcttcc acacattctg tcgcttcacc gtttgacttg
     2761 atggtcatgg atgaccaggg gaactgacag aattccagcc agtgaatgga ttgtgtgacc
     2821 tgttgggtat cttcgtacca catttggtcc tggctcttga gataaacata aacatccaaa
     2881 cctttaaagg cttctttcaa gcgttcaaac tcgtctgcct gccagggttt gttgagatta
     2941 atcatggtaa tcacaatggt tgtctggtaa ttgcgttggg ctttgagctc caagagttta
     3001 aggatatttt tgtaagactc agtaaagtta gacgctttgc cacgcacttc tttatggcga
     3061 tcgtcgtcaa cgctctcaat ggaatacttc acataaccca ggccattttc aaaggtttca
     3121 atcgtgcgtt ccatattaat attcgcagga ttacacgaga agtagctttc aaggccgcgt
     3181 tcggtcatcc atttgacgta ttgtggcatg tttttatcca acagtgggtc gccatagcca
     3241 tgcaggacga taacgcgtgg aatgacatac aggaaaaagt ggttctcact catatcattt
     3301 ttgtgaatgc cataattttc cgtcacaaat ttttcccagc gattgagttg ctcggtgctg
     3361 aaaggtttca gctggtcaat cacgcgtttg aaaagttcgg gcgacatggt ttcgatgggt
     3421 cgggtcatca tggttgtgcg ggggcacatt tcacaacgca tattacaggc gttggttgtt
     3481 tctatgttgt aaacgaccgg atcagggctg cgaaatgctt caaattgctc ttccaaaaag
     3541 gcgcggggca ttttttcgcc attcatcaga cgttttttaa ggtcatatac tttcatgtag
     3601 aattcaatat caatcatttt tagtctccat tcagggtttc atggtgggat atcgtttgcc
     3661 aatgtcttat gagcatatca caaagttcgc gcaaagcacc gcgcccacct tttttagtca
     3721 aaattaaatc tgcccgtcgt ataacttcgg ggtgagaatc agccggcgcg acaaaatagc
     3781 cagccacgtc cagagttggc aaatcgttga cgtcgttgcc aacgaagatg gtttcttcag
     3841 cgcgcatacc ccgtttgcgc atctcgtcac gcaaagcggt ggctttgtca tcaatcgcct
     3901 gtaagacagg gatgcccaac tttttggccc gctgggcaac gaccaaattg cgctcttttg
     3961 agataatcat acattctacc ggcgtttgcg cttttaagag accgaaacct aagccatctg
     4021 aacggtccgc gcgtacgctt tcaagtccat tttcatcgac aatcacacgg ttatcggtaa
     4081 aaacgccgtc gaaatccatc accaaaaaac gcggtcgggc aggaaaagcg cgacgtaaat
     4141 tagccgggtc cacaacttca gcggccagtt cgcgcaaggt cgcttcagca accggccagt
     4201 cgctggggat atcaatatcc acggtaaatt tcgggtcaat gtaaagcggg tagatgacgt
     4261 cgccactaag cgactctttt tcaaaaacag atgcgacccg tatcgcgtca atatgacctg
     4321 tttgccagaa agtatccggc aaggcctggc gtggcgcgtt atagggctct ttgatacctg
     4381 ggacagtcag cagggttttc atttcgcctg tgtcggggtc aatggtccac atcttaaagg
     4441 ggttttgggc gcttggcacc acgccacgca cactgtccgc ttcgggatgc gccagcaaga
     4501 tgtctatcgc gtcatctacc agcctcgtgg ggcgtacagg cgaagtgggg cgcagttgca
     4561 cgactacatc agggtgatag ttttcatgct ctgccagcca ctctaaagcg tgcacaatga
     4621 cggggtaatc ggtggaatgg tcctgggcta gttccgcggg acgtataaag ggcacttcgg
     4681 ctccccactc acgcgagatt cgggcaattt cctcatcgtc ggttgataca acaacccggc
     4741 tgacatgctc tgaacgcaag gcagccaaaa tactgtacgc aatcaaagga tatccggcga
     4801 aaggtttgat gttttttccg ggaatacctt ttgaaccgcc ccgcgccggg atgatagcca
     4861 agacttcttg tttttctacc atgctgtcca ccctccatct accacgagat tactgccggt
     4921 catgtagcta cttgcatcgg aacataaaaa gagcatcgcg gcgtccatct catcgatgtc
     4981 tgccatgcgt cccatgattg ttttcgtagc gtagttgcgt gaaaagtcat cattgtggcc
     5041 gttgaaaatg ccaccgggag a
//
LOCUS       DAOSFC010000084         5031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_14842_length_5031_cov_5.391680, whole genome shotgun sequence.
ACCESSION   DAOSFC010000084 DAOSFC010000000
VERSION     DAOSFC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14842_length_5031_cov_5.391680"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PK791_03180"
     CDS             <1..369
                     /locus_tag="PK791_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92349.1"
                     /translation="DAVILSATFPGEHVDSSESLEKLDALTAKRAQAQPKGHSFGSTF
                     KNPSNDFAGRLLEEAGMKGFVYGKARVSDKHANFILNDGEASAQEIYTLIRMGQARVK
                     EKFGIDLQTEIELIGDFDEF"
     gene            359..1462
                     /locus_tag="PK791_03185"
     CDS             359..1462
                     /locus_tag="PK791_03185"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOH92350.1"
                     /translation="MSSKRLNLALIFGGRSGEHEVSVMSARSVLQAIDTDKYQVWQIG
                     ITREGHWLTGENTLQAFENKSCESLKQVVLMQIDDKTVLHQLVDGNLQKISAIDVFFP
                     ILHGTYGEDGTIQGYFEMLDVAYVGAGLLSSSVGMDKGICKDLVANLGVPVLEYKIFN
                     RQKIETDLEATALEAEKIAPYPLFVKPANLGSSVGISKAKNRQELIKGLEIAAKYDRR
                     VLVERGINAREIEVSVLGNTDPIVSTPGEIIPGDEFYSYDDKYIDGIARTFVPADLSD
                     AMTQRVQEIALQVYQGIDCAGMARVDFLIDKHSEEIFFSEINTIPGFTPISMFPKLLA
                     HGGITYPELIDRLIALALERKADRDKTIRSIQA"
     gene            1462..2511
                     /locus_tag="PK791_03190"
     CDS             1462..2511
                     /locus_tag="PK791_03190"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOH92351.1"
                     /translation="MARKPEELSRADQIRARRHIEPVKRKPIERAPQESTQRAARSAS
                     RVVTRRQYTDTQATPQAESSRGNRPVYIPTGTPGKELRLPAVPRVSVGMRVFSGILAL
                     LLLVGVISMLKSDNFKVNPNGINIRGNFRVSNAEIASKLSSYGTRIIEVEPDKIEQVI
                     IRDFPDIKTVKVGVSLPANLNVLVVERIPSIIWFVDATEETEASQIWIDDEGYSFEVR
                     GAASLPIEVHANTAPPPPFKPVEMEKPTAPEETETEEEIFKEPEPNVDPRLVEAIQAL
                     NQLKPQGTQLRYDARRGLGWVDPNYGWMVYFGRSAEHLDLKMAQYQKIAELILERNLQ
                     PTLISLEFLHAPYFR"
     gene            2522..3757
                     /gene="ftsA"
                     /locus_tag="PK791_03195"
     CDS             2522..3757
                     /gene="ftsA"
                     /locus_tag="PK791_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOH92352.1"
                     /translation="MDEPIVVGLDVGTSKIVTLVARIENGTTMRVLGVGVEPSHGIRN
                     GNVVDINAASDAIARSIEKAERTSGYEINSAIVSLAGAQVSSTNSKGIVGISGRVIDQ
                     EDVFRALDSAQAIAIPHNREIIHVIQRGFIIDGQEGIRQPVGMHGYRLEVETHIITAS
                     SSTINNLRQCVSQAGIDVSDFVLNPLAAGEAVLSETERNLGVALVDLGGGTTDIAIYV
                     NGDIWYANVLQIGGYHVSSDIAQGLRLPLDVAEEVKKQHGHAVLDDIGEKETFNIVTF
                     GSEQSTKIERRELVNIIEARVEEIMEMVEQEIKRSGYDVMLPAGIVLTGGGAEMPGIR
                     KLANRITGLPVRIGKPENIVGLTDQISNPACATSVGLLQWALLMTETHQILPEQSGES
                     KPQKAKAEGFLGWLRKILP"
     gene            3787..4962
                     /gene="ftsZ"
                     /locus_tag="PK791_03200"
     CDS             3787..4962
                     /gene="ftsZ"
                     /locus_tag="PK791_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOH92353.1"
                     /translation="MEAKQTESYARIKVVGVGGGGCNAVNRMIAEGLHGIEFIAINTD
                     AQALLKSQAQTRVRIGDKSTRGLGAGGNPEVGQAAAEESAEELYEVLKGADMVFVTAG
                     LGGGTGTGAAPVVAQIAKEIGALTIGVVTRPFAFEGAHRAKSAETGAQKLKEHADTLI
                     VIPNDRLLQIVGKNIGLNDAFRMADDVLRQGIQGISELITIPGLINLDFADVRTIMSE
                     GGAALMAVGVASGEDRARIAAEQAISSQLLDVTINGAHGILFNVTGGPNLSLFEVNQA
                     AAIIKETASEDVNLIFGAVIDEKMGDEIRITVIATGFDRNRPQLTEDQAESFLIPNTQ
                     MGQVGQQSPFLSRSEYKHQAELTHTAKPKAQQEYVYTSEVDLNLPAFIRRQQANRGK"
BASE COUNT         1303 a         1374 c         1260 g         1094 t
ORIGIN      
        1 gacgcggtca tactttccgc gactttcccc ggcgaacacg tcgactcaag cgaatcgctt
       61 gaaaagctcg acgctctcac cgccaaacgt gctcaagccc agcccaaagg ccacagtttt
      121 ggctcaacct tcaagaaccc gtccaacgat tttgctggtc gtctgcttga agaggctggc
      181 atgaaaggtt ttgtttacgg caaagcacgc gtgagtgaca agcatgccaa cttcattctc
      241 aacgatggcg aagcttccgc gcaggagatt tacacgctca ttcgcatggg tcaggcgcgt
      301 gtcaaggaaa aatttggaat cgatttacaa accgaaatcg agttaatagg agatttcgat
      361 gagttctaaa cgcttaaatc tggctttgat atttggtggt cgctccggtg aacatgaggt
      421 ctcggtgatg tccgcgcgct cggtgctgca ggcaatcgat accgacaaat atcaggtctg
      481 gcaaatcggc atcacccgcg aaggccactg gctgaccggt gaaaacacac tccaggcctt
      541 cgaaaacaag tcctgcgaga gtctgaaaca agtcgtttta atgcaaatcg acgataaaac
      601 agtcttacac cagcttgtgg acggaaatct gcaaaaaatc agcgcgattg acgtcttctt
      661 ccccatcttg catggcacct atggcgaaga tggcaccatt caaggctatt tcgagatgct
      721 cgacgtagcc tatgtgggcg ctggcttgct tagctcatca gttggcatgg ataaaggcat
      781 ttgcaaagac ctcgtcgcga atctcggcgt ccccgttttg gaatacaaga tttttaaccg
      841 tcaaaaaatc gaaaccgacc tggaagccac agcgctggaa gctgaaaaga ttgcccccta
      901 cccgcttttc gttaaaccgg cgaatcttgg ctcatcggtg ggcatcagca aagccaaaaa
      961 ccgccaggaa ctgattaaag gcctggaaat cgccgcaaaa tacgaccgtc gcgtgctcgt
     1021 tgaacggggt attaacgccc gcgaaattga agttagcgtg ctgggcaaca cagaccccat
     1081 cgtatcgaca cccggcgaga ttatccccgg tgatgaattc tacagctacg acgacaaata
     1141 cattgatggc atcgccagaa ctttcgttcc cgcggattta tccgacgcca tgacccaacg
     1201 cgtgcaggaa atcgccctgc aggtttatca gggcatcgat tgcgcgggaa tggcgcgcgt
     1261 tgacttttta attgacaaac acagcgaaga aatcttcttc agcgaaatta acaccattcc
     1321 cgggttcacc cccatcagca tgttccccaa actgcttgcc cacgggggca tcacgtatcc
     1381 ggaattgatt gaccgcctta ttgcgctggc acttgaacgc aaggcggacc gcgataaaac
     1441 gataaggagt atacaagcct aatggcacgc aagcccgagg aactctcacg cgcggaccaa
     1501 attcgcgcca ggcgacatat cgagcctgtc aaacgcaagc ccattgagcg cgcgccgcaa
     1561 gaaagcaccc agcgcgccgc caggtctgcg tcgcgtgtgg ttacccgacg gcaatacacc
     1621 gacactcagg cgacacccca ggctgaaagc agtcgcggta accggccagt ctacatcccc
     1681 accggcacac ctggcaaaga gctgagactc ccggctgtgc cccgcgtgag cgttggcatg
     1741 cgcgttttct ccggcatatt agcacttttg cttttggttg gggttatcag tatgctcaaa
     1801 agcgacaact tcaaggtcaa ccccaacgga atcaacattc gcggtaactt ccgtgtcagc
     1861 aatgctgaaa tcgcctcaaa actcagctca tacggcacgc gcattattga agttgaaccg
     1921 gacaaaatcg aacaagtcat tattcgcgac ttccccgata tcaaaacagt aaaggtcggg
     1981 gttagtttgc cagctaattt aaacgtactg gtcgtggaac gcattccttc catcatttgg
     2041 tttgtcgacg cgaccgaaga aacagaagcc agtcaaattt ggatagacga cgaaggctat
     2101 agctttgaag tgcgtggcgc ggccagcttg ccgattgaag tgcacgccaa caccgccccg
     2161 ccacctccat tcaaacccgt ggaaatggaa aaaccgaccg caccagaaga aacggaaaca
     2221 gaagaagaaa ttttcaagga acctgaaccc aacgttgacc ccagactggt tgaggctatt
     2281 caggcactca atcaactcaa accccagggc actcagttgc gctatgacgc caggcgcggc
     2341 ttgggttggg tagacccaaa ttatggctgg atggtctact ttggcagaag tgccgaacat
     2401 ttggatttaa agatggcgca atatcaaaaa atagcggagc tgattttgga aagaaacttg
     2461 caaccgactc ttattagcct tgagtttttg cacgctccgt attttcgcta ggagttttat
     2521 tatggatgaa cctattgttg tcggattaga tgttggaaca agcaaaatag ttaccctggt
     2581 cgcccgtatt gagaatggca ccactatgcg tgtcttgggc gtgggtgtgg aacccagcca
     2641 cggcattcgc aacggtaatg ttgtggacat caacgccgcc tcagacgcca ttgcccgctc
     2701 catcgaaaag gccgaacgca cctccggcta cgaaatcaac agcgcgattg tcagcctggc
     2761 cggcgcgcag gtgagttcca ccaacagcaa aggcatcgtt ggtatttctg gccgggttat
     2821 tgaccaggaa gatgttttcc gcgcgcttga ttctgcccag gctattgcta tcccgcataa
     2881 ccgcgaaatt atccatgtta tccagcgcgg ttttatcatt gacggccagg aaggcattcg
     2941 ccaaccggtt ggcatgcacg gttacaggct ggaagtcgaa acgcacatca tcactgcctc
     3001 atcatccaca attaacaatt tgcgccagtg cgtatcgcaa gccggcatcg acgtcagcga
     3061 ctttgtgctc aatcccctgg cagccggcga agctgtgctc tccgagactg aacgcaattt
     3121 gggcgtcgcc ctggtcgatt tgggcggggg caccaccgat attgccattt acgtcaatgg
     3181 tgacatttgg tacgccaacg tcctgcaaat cggcggttat cacgttagct cagacattgc
     3241 ccagggcctg cgcttgcccc tggatgttgc cgaagaagtc aaaaaacaac acggtcacgc
     3301 cgtgctggac gacatcggcg aaaaagagac ttttaatatt gttacctttg gctccgaaca
     3361 gagcacaaaa atcgaacggc gcgagttggt taatatcatt gaagctcgcg ttgaagaaat
     3421 tatggaaatg gtcgagcagg aaatcaaacg ctcgggctac gacgtgatgt tgcctgctgg
     3481 tattgtactc accggcggtg gcgctgaaat gcccggcatc agaaaacttg ccaaccgcat
     3541 cactggtttg ccagtccgta tcggcaagcc cgaaaacatt gttggtctta ccgaccaaat
     3601 cagcaatcca gcctgcgcaa cgagtgttgg actcttgcaa tgggcccttt taatgacgga
     3661 aacacaccag attttgcccg aacaatctgg agaatcaaag ccccaaaaag ccaaagcgga
     3721 aggctttctt ggttggctac gcaaaatttt accttagaca ggttttatta tagaggagaa
     3781 taaaaaatgg aagcaaaaca aactgaatca tatgcacgaa tcaaagtcgt cggcgtcggt
     3841 ggtggcggat gtaacgccgt caaccgcatg attgccgaag gactccacgg tattgaattc
     3901 atcgcgatca ataccgacgc ccaggcgctg ctcaaatccc aggcgcaaac ccgtgttcgc
     3961 atcggcgaca agtccacccg tggtcttggt gctggtggta accccgaggt tggccaggca
     4021 gccgctgaag aatcagccga agaactttac gaagtgctca aaggcgccga catggtcttt
     4081 gttaccgccg gtcttggtgg tggcactggc accggcgcgg cacccgttgt ggcacaaatc
     4141 gccaaggaaa tcggcgcctt aactatcggt gtggttaccc gtcctttcgc atttgaaggc
     4201 gcgcaccgtg ccaaatcagc cgaaaccggc gcgcaaaagc tcaaagaaca cgccgacacg
     4261 ctgattgtta tccccaacga tcgtcttttg caaatcgtag gcaaaaacat cggactgaac
     4321 gacgccttcc gcatggcgga tgatgtcctg cgtcagggta tccagggtat ttcagaactg
     4381 attaccattc cgggcttaat caaccttgac tttgccgacg ttcgcaccat tatgtccgaa
     4441 ggtggcgccg cgctgatggc tgtcggtgtt gccagtggcg aagaccgcgc ccgaatcgct
     4501 gccgaacagg ccatttccag ccagcttttg gatgtcacca tcaacggcgc gcatggtatc
     4561 ttgttcaacg ttaccggtgg tccaaacctt tcgctctttg aagtcaacca ggctgcggca
     4621 atcatcaaag aaaccgccag cgaagacgtc aacctcattt tcggtgccgt tatcgatgag
     4681 aagatgggcg acgaaatccg cattactgtt attgccacgg gtttcgatcg caaccgcccc
     4741 cagctgacag aggatcaagc ggaatccttc ttgatcccaa acacccaaat gggccaggtt
     4801 ggccagcaaa gtcccttctt aagtcgctct gagtacaaac accaggctga actcacccac
     4861 accgcaaaac ccaaagccca gcaagaatac gtttatacca gcgaagttga cctcaacctc
     4921 cccgctttca tccgccgtca gcaagccaac cgcggtaaat aagcgacata aaatgtgctt
     4981 cccaaatcgt cctttcccgt gatgagaaag gacgattttg cacgtttgtt t
//
LOCUS       DAOSFC010000085         4980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_15037_length_4980_cov_4.666599, whole genome shotgun sequence.
ACCESSION   DAOSFC010000085 DAOSFC010000000
VERSION     DAOSFC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15037_length_4980_cov_4.666599"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1316)
                     /gene="ricT"
                     /locus_tag="PK791_03205"
     CDS             complement(99..1316)
                     /gene="ricT"
                     /locus_tag="PK791_03205"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOH92354.1"
                     /translation="MEDLKIIGVQFTPVGKHYHFSIPEEAQLSLGDAVIVNTSRGLQL
                     GTVAALNVDPKIDGPNMLKVERLATEEDFESYKNYKKKAKEAIDIINRFLREGEFGEI
                     KAVDAEYTLDGAKMTIYLTYEQNVEFDVRSFLRDISYNFNDTRLEVRQVGPRDAAKMI
                     SGLGACGIEKRCCSRFLTEFTSISIKMAKAQDISLTPTEITGICGRLRCCLRYENETY
                     EAALQELPKRKKKVMTPLGEGRVTQVLPLRGSVIVNIPEIGPRQFTQEELETGVLQVK
                     PVVFDDIPDEYNIDHSDVELLNIEPERKPKGRRNSKQTKREGERKERAKTIRSDSEKP
                     GPETTQNAPAQKKREDGGKRNYSRNKRRNSERTASKNEVSKDKPVANQPAKNREQKPS
                     RKPRSQRRQDNKR"
     gene            complement(1472..2845)
                     /locus_tag="PK791_03210"
     CDS             complement(1472..2845)
                     /locus_tag="PK791_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH92355.1"
                     /translation="MQYSGYTINRSDRIFVTGHINPDTDSIAAAIGYAWLLRERDNLP
                     TVACRAGSVNLQTAWVLKTLNLEQPILLTDASPRFESVTKRYDVATPDKSLNEAWAIL
                     TRTGGIAPIVNADGTPYGMVTGKSVFDFLRRIIGPHPRFRDMTISELLDIPSREAANT
                     DIPRFQPQTRIKDVINRLLRHEYTEYWVVDEKGRYLGVVRQNNLLHPPRIKVILVDHN
                     EPQQSIASLEEAELLEILDHHRLGNRPTHVPIKFTVDIVGSTSTLVAEQIVEAGLSAP
                     PAIAGILLAGLLSDTLIFQSPTTTPRDKEVAQTLGRWAFVPGSPLENETVQSYGEKVV
                     RAGAGLLTREPDNIVNGDVKTYETAGVKFAISQVEVAGVYDLGDVLGQLRRALIKYRD
                     EKGLDFAMLMITDVVEGSSRLLMVDQPLVLDELPYRILRDGTFLAEGVVSRKKQLLPQ
                     VLSLLEE"
     gene            complement(2905..3315)
                     /gene="mce"
                     /locus_tag="PK791_03215"
     CDS             complement(2905..3315)
                     /gene="mce"
                     /locus_tag="PK791_03215"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOH92356.1"
                     /translation="MTIKKINHVAIAVKDIEAALDFWENKLGLKLDYIEEVPSEASRV
                     AFIPIGDSLIELVQPTRDDTGMAAYLEKRGEGMHHLCVEVENIDETFNRLKEQGVRLI
                     NTEPQVLPGRKMAFIHPKAANGVLVELYELTDTK"
     gene            complement(3358..>4980)
                     /locus_tag="PK791_03220"
     CDS             complement(3358..>4980)
                     /locus_tag="PK791_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOH92357.1"
                     /translation="EKIYNPGKARFPERKAEFKTSSNIEVPPVIHPEDIVMDREADLG
                     YPGQYPFTRGVQPTMYRGRLWTMRQYAGFSSAKESNERYRFLLEQGQTGLSVAFDLPT
                     QIGYDADDKIAMGEVGKVGVSISSIEDMEILLKDIPLDRVSTSMTINAPAAVLLAMYI
                     AVAKRQGVAPEKLRGTIQNDILKEYIARGTYIFPPAPSMRLITDIFQYCKEYVPNWNT
                     ISISGYHIREAGSTAAQEVAFTLANGIAYIEAAVKSGMDVDEFASQLSFFFNAHNNFL
                     EEIAKFRAARRLYARIMKERFGAKKASSQKLRFHTQTAGSTLTAQQPENNVVRVTLQA
                     LAAVLGGTQSLHTNSMDEALWLPTQKAVQVALRTQQIIAYESGVADTIDPMAGSYIIE
                     HLTDEIEKQAVEYIRKIDEMGGAMKAIEQGYLQNEIQDAAFAAQRNLEDGEDIVVGVN
                     RFQVDETITLESLKIDQSIELEQRERLAKLRASRDNNKAEDLLNRLEACAKGDENMMP
                     IIIECVEGKLTLGEICGRLRQVWGEYRAPSFL"
BASE COUNT         1255 a         1162 c         1029 g         1534 t
ORIGIN      
        1 gcctcatctt gtaacagtaa tcagttatac cttgtgtatt atttattact gacatttaac
       61 tcaatcaaat ctgttgccac atttattgca ctaatttttt agcgtttgtt atcttgccgg
      121 cgttgacttc gcggtttccg agatggcttt tgttcacggt tcttggcagg ctgatttgca
      181 actggcttat ctttacttac ttcatttttt gaagctgtgc gttctgagtt gcgccttttg
      241 ttcctcgaat aattgcgctt tccaccatct tcgcgttttt tttgcgctgg ggcattttgc
      301 gttgtttccg gtcctggctt ttcagaatca gatcgaattg ttttagctct ttcttttctc
      361 tcgccttccc gttttgtctg ttttgaattt cgtctgcctt ttggtttacg ttcaggttca
      421 atattaagca actcaacatc agaatgatct atgttgtatt catcaggaat gtcatcaaaa
      481 acgactggct taacttgcaa cacgcccgtt tcaagttctt cctgcgtaaa ctgacggggt
      541 ccaatttcag gaatattaac aatgaccgaa cctcttaagg gcaaaacctg ggttacccga
      601 ccttcgccca gtggtgtcat tactttcttt tttcgctttg gcaattcctg aagcgccgct
      661 tcataggtct cgttttcata acgcaagcaa catcgcaagc gaccgcagat gcctgtaatc
      721 tcggttggtg ttagcgaaat atcctgtgct tttgccatct taatggatat tgatgtaaat
      781 tcagttaaaa accgtgagca acatcgcttt tcaatgccac aggcaccaag tcctgatatc
      841 atttttgcgg catcccgggg acctacctga cggacttcca aacgggtgtc attaaaatta
      901 taagagatat cgcgcaagaa ggaacgaaca tcaaactcca cgttttgctc ataagtcaga
      961 tagatagtca tttttgcgcc gtcgagtgta tattcagcat caacggcttt aatttcgcca
     1021 aattcgcctt ctctcaaaaa gcgatttatt atgtcaatgg cttctttagc ttttttcttg
     1081 taatttttat agctttcaaa gtcttcttca gtagccaatc tttctacttt aagcatattt
     1141 ggcccatcaa ttttggggtc aacgtttaaa gccgctacgg ttcccagctg taagccccgt
     1201 gatgtattta caataacagc gtccccaaga gaaagctggg cttcttctgg gatagaaaag
     1261 tgataatgtt taccaacagg cgtaaactgt acgccaataa tctttaaatc ttccatattt
     1321 aaattatact agatgcctgc acctgtttcc aaaacttcaa tttcccggaa ggaaaacatg
     1381 gcaaaaatga tttcatatta actaaatgcc agatatattt aaaaactggc attcaagaaa
     1441 tctcatatat agctataact gatctgctag cctactcctc caaaagcgat aatacctgcg
     1501 gcaaaagttg ttttttacgt gatacaacac cttccgccaa aaaggtacca tcacgcagta
     1561 tccggtatgg caactcatca agtactaatg gttgatcaac catcaacaaa cgacttgaac
     1621 cttccacaac gtctgtaatc attaacatgg cgaagtcaag ccctttttca tcacgatact
     1681 ttatgagcgc gcgtcttagt tgtcctaata catctccaag atcatataca ccagcaactt
     1741 caacctgaga tatagcgaat tttacgccag cagtttcata agttttcaca tcaccattca
     1801 cgatattgtc aggttcacgt gtaagcaagc cagcgccggc acgaacaacc ttttcgccat
     1861 aactttgcac agtttcattt tccagtggtg aacctggaac aaatgcccat cttcccagag
     1921 tttgtgcaac ttctttgtca cgtggggttg ttgttggtga ttgaaatatc aaggtgtctg
     1981 aaagcagacc tgccagcaga ataccagcaa tagccggtgg cgcgcttaat ccagcttcca
     2041 caatttgctc ggccaccaaa gtcgatgtgc ttcccacaat atcgaccgta aattttatgg
     2101 gcacatgggt tggacggttt ccaagacgat ggtgatctaa aatttccagc aattcggcct
     2161 cttccaacga cgcaatcgat tgttgcggtt cgttgtggtc taccaaaatg actttgatgc
     2221 gtggtggatg caacaaattg ttttgacgaa cgacacctaa atagcgtccc ttttcatcaa
     2281 caacccaata ttcagtatat tcgtgacgta aaagacggtt tataacgtct ttgatacgcg
     2341 tttgtggttg aaaacgaggg atatcggtgt ttgctgcttc ccggcttgga atatccagta
     2401 gttcggatat ggtcatatcg cgaaatcttg gatgtggccc gataatacgt cgcaaaaaat
     2461 caaaaacgct ttttccagtc accataccat aaggtgttcc gtcggcattg acaatcggcg
     2521 caatgccacc ggtgcgggtt agaatcgccc atgcttcatt caaagattta tcaggcgttg
     2581 caacatcgta acgtttggta actgattcaa aacgtggcga tgcgtcagtc agcaatatgg
     2641 gttgttccag gttaagcgtt ttgagaaccc acgcagtttg caggtttacc gaaccagcac
     2701 gacaagcaac cgtcggcaaa ttatcccgtt cgcgcaaaag ccaggcgtaa cctatcgctg
     2761 ccgcaatcga gtcggtatca ggattgatat gtccagttac aaaaattcta tctgatctgt
     2821 ttattgtgta tccactatac tgcatggttc ttcctcaatc tttagttaaa aagccaggat
     2881 tttagacctg gcttataaaa caccttattt ggtgtcagtc aactcataga gttccacaag
     2941 tacgccattg gccgctttgg gatgaataaa cgccattttt ctgcctggta gcacttgtgg
     3001 ttctgtgttg ataaggcgta cgccctgttc ttttagtcta ttgaatgtct catcaatatt
     3061 ttctacttca acgcaaagat gatgcatgcc ctcacctcgt ttttcaagat aagctgccat
     3121 gcccgtatca tcacgggtag gttgcaccaa ttctattaag ctgtcaccga tgggaataaa
     3181 ggcaactctg gaggcttcgg atggtacttc ttcaatgtag tcaagtttca aacccagttt
     3241 attctcccaa aagtctagcg cggcttcaat atcttttact gcaatagcga cgtggtttat
     3301 tttttttatg gtcattttct ctccgttata tttttagttt tgttagttcc tttagcttta
     3361 caagaaactt ggcgcgcgat attcgcccca aacctggcgt aagcggccgc atatttcgcc
     3421 cagggtcagc tttccttcta cacattcgat gatgatgggc atcatattct cgtcgccttt
     3481 ggcacaagct tcaagtcggt taagaaggtc ttcagccttg ttgttgtcgc gtgatgcccg
     3541 taattttgcc aggcgttcgc gttgttccag ttcaatagac tggtcaatct tgagtgattc
     3601 caatgtgatg gtttcatcaa cctggaatcg attaacacca acgacaatgt cttcaccatc
     3661 ttcaagatta cgttgagcag caaacgcggc atcctggatt tcattttgca ggtagccttg
     3721 ttcaatggct ttcattgcac cgcccatttc atcgatcttg cgaatgtact ctactgcttg
     3781 cttttcgatt tcatcagtaa ggtgttcaat gatgtaagaa ccagccatgg ggtcaattgt
     3841 gtctgccact ccactttcgt aagcaataat ttgttgtgtg cgcaaggcga cctgtaccgc
     3901 tttctgtgtg ggcagccaaa gtgcctcgtc catactgttg gtatgcaggg attgcgtgcc
     3961 tcccaataca gcggcaagtg cttgcaaagt cacgcgcaca acattatttt ccggttgttg
     4021 cgcagttagt gttgaccctg cagtttgggt atggaaacgt aatttttgtg agctcgcctt
     4081 ctttgcgcca aagcgctctt tcataatgcg cgcgtaaagc ctgcgcgcgg cgcggaactt
     4141 ggcaatttct tccaggaaat tattatgagc attgaagaag aaagacaact gactggcaaa
     4201 ttcatcaaca tccatgccag atttaacggc agcttcaatg taagcgatgc cattagccaa
     4261 agtaaaggcg acttcctggg cagcggttga acccgcttcg cgaatgtggt atcccgagat
     4321 gctgattgtg ttccagttgg gcacatattc cttgcaatac tggaaaatat ccgtaatcaa
     4381 gcgcatactg ggtgccggtg ggaagatata ggtacctcga gcgatatatt ctttcaaaat
     4441 atcgttttga atggtaccgc gcaatttctc tggggctacg ccttgtcgtt tcgccacagc
     4501 aatatacatc gccaataaca ctgccgcggg cgcgtttatc gtcatgcttg ttgaaactct
     4561 gtccaatgga atgtctttta gcaatatttc catatcttca atggatgaaa tagacacacc
     4621 aactttaccc acctcgccca ttgctatttt gtcatcagca tcataaccaa tttgggtcgg
     4681 caggtcgaaa gccacagata agcctgtttg accctgttcc agcaagaagc gatagcgttc
     4741 attggattct ttcgcgcttg aaaaaccagc atactggcgc atcgtccata aacgtccccg
     4801 atacatggta ggttgcacac cacgggtaaa aggatattgc cccgggtagc ctagatcggc
     4861 ttcccggtcc attacaatat cttcaggatg aattaccgga gggacttcaa tgtttgaact
     4921 tgtcttaaat tctgcttttc tttcagggaa acgggctttt ccgggattat agattttttc
//
LOCUS       DAOSFC010000086         4973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_15058_length_4973_cov_5.227328, whole genome shotgun sequence.
ACCESSION   DAOSFC010000086 DAOSFC010000000
VERSION     DAOSFC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15058_length_4973_cov_5.227328"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PK791_03225"
     CDS             <1..726
                     /locus_tag="PK791_03225"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOH92358.1"
                     /translation="EDGLDRLLHPGASYSLSAELLNLIKENIRQEKPKSSGESHQKFA
                     PDHIKLYVPFERYDAWQNLRPVLNEVAAEIEQSFTAEGYKFERKPRIQIIASEDLPLD
                     QIDITTSYYHEIEQTGKTSLIQLDNTPRNIPLPSGACFIVNGKDNIPLQKPIVHIGRH
                     SNNDIVIQDPMVSREHLQLRAQRGYYLLFDLSSTGGTTINGQSVHTATLKPGDVVRIG
                     QTVLIYNQDLPETTTKTIVSVPE"
     gene            726..1172
                     /locus_tag="PK791_03230"
     CDS             726..1172
                     /locus_tag="PK791_03230"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOH92359.1"
                     /translation="MTPLVGLILRILSIVMLCVFLGITIWLLWRAVSAKEATAGRLAI
                     PTISLVSDEDGVREELTFNSQEVTIGRSPECDFTLNNHTVSARHGRLNFHLNQWWYED
                     LKSTNGSFLQGMRIEEPIVIKDGDEIAFGDVYVSVFIKAVDNIKQR"
     gene            1175..2059
                     /gene="mtnP"
                     /locus_tag="PK791_03235"
     CDS             1175..2059
                     /gene="mtnP"
                     /locus_tag="PK791_03235"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOH92360.1"
                     /translation="MTEEILLGVIGGSGLYSFEELENQESIEIDTPFGKPSSPIVIGE
                     IKGQKIAFLARHGIGHVYSPTDVNYRANIYAFKSLGIRAIVSVSAVGSLREDYAPGHI
                     IVPDQIYDNTKDRKRSFFGEGMVVHISSADPFCPALSQHLLKALREQDAIVHEGGTLI
                     TIEGPRFSTRGESETFRKWGMSLVGMTAAPEAFLAREAEMCYATMAHVTDFDCWHVSE
                     EAVTVEMVVNTLQKNTRTAQKSIVRLVELLAEAQSAGKVSYCECEHALKDAIMTNPNA
                     ICEDKKQTLELLTGKYFK"
     gene            2066..4666
                     /locus_tag="PK791_03240"
     CDS             2066..4666
                     /locus_tag="PK791_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOH92361.1"
                     /translation="MNNDLKAPRQPRLILFGILFVFFYTAALSLASAIRTHAPGFQVD
                     WYYFIPFAAWLIGALGLDYAVKRFLPNRDPWIFPVVMLLCGWGLLTIWRLSPTLGQRQ
                     IIWFLLACLLFYFGIRQKELVSKLKGLKYLWLLFGLLLIGLTFWIGVNPSGSGPTRWL
                     SAFGIFFQPSEPLKLFLLVYIAAYFADQVKPNTSLIASILPTLIIIAVIGVMLISQRD
                     LGTAALFLGFYVLMLTVSSNSRKYLWIVPVLAIIAIVVGYFTLDIVKTRVDIWLNPWL
                     NTSGSSYQIAQAQIAVASGGLYGTGPGLGSPGFVPVAVSDFIFTAIAEETGLIGTSAL
                     LILFAIVIIRGINIALASKTTYGRYLAFGISMFLTLQCLWIICGNLGLLPLTGVTLPF
                     MSYGGSSLVTSILAILLLLKISADSSTIPLPDKARKPYHRIAAIVLGIFVLIIGWNSY
                     LAIFNQDAIVNKAENMRWAIDDRYSPLGNISDQSGNPIVRTIGTPGDYERELLVPGLS
                     ITIGYTNPVLGKSGLERSLYPYLRGTETRDETLSSQYQLLYNQPPPGSDVSVNIILDL
                     QAKADKLLGDKHGAIILMNAVNGEIYAMVSHPYFDYTSIAEDWDSWRQREDAPLLNRV
                     TQGVYPLGTLANGFALSSYWVQEGTNTLSIPLHSDAIDPLCGKMLTVTDQVNPIQHGC
                     QEATRDLINANDPLALTEQLDKFGFYSIPSFSLELAQPTSRPGKNYFNNIQTLIPATQ
                     LSPLQMALIAASITNDGDKITPRLVNAYQTENGSWKSYTEPSQAMRVLPQTVAKTTRD
                     YFSQHNKAYWYMMGHSAMEEEQTIVWYVGGTNDAWTGTPMVIVIALEDSQPDLAYRIA
                     DQLFVPQSED"
     gene            complement(4765..>4973)
                     /locus_tag="PK791_03245"
     CDS             complement(4765..>4973)
                     /locus_tag="PK791_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOH92362.1"
                     /translation="EVLLEAGKHTVLDVLVDQGLVSSRSEGRRVISQQGVKLDGVTVG
                     DPTAVLDTGGVLQVGKRKFVRILI"
BASE COUNT         1269 a         1369 c         1135 g         1200 t
ORIGIN      
        1 gaagacgggc ttgaccgtct tttgcatccc ggcgcgtctt actcccttag cgcggaactg
       61 ctcaatctga ttaaagaaaa tatccgccag gaaaaaccca aaagcagtgg cgaaagccat
      121 caaaaattcg cgcctgacca catcaagctt tacgtgccct ttgaacgcta tgacgcctgg
      181 caaaacttgc gccccgtcct gaacgaagtc gccgcggaaa tcgaacaaag cttcaccgcc
      241 gaaggttaca agtttgaacg caaaccacgt atccaaatca tcgcgtccga agatctaccc
      301 ctggaccaaa tcgatatcac caccagctac taccacgaaa ttgaacagac cggaaaaact
      361 tccctgattc aactggataa tacccccaga aatattccgt tgccaagtgg agcttgtttt
      421 atcgtcaatg gcaaagacaa tatcccactg caaaagccga ttgttcacat cggacgtcac
      481 agcaacaatg atattgtcat tcaagacccc atggtctcgc gtgagcattt gcaacttcgc
      541 gcgcaacggg gctattacct gctttttgat ctcagcagta ccggtggcac aaccatcaac
      601 gggcaaagcg tgcacaccgc caccttgaaa cctggtgacg tggtacgcat cggacaaacc
      661 gtgctcatct acaaccagga cttgcccgaa acaacaacca aaacgattgt gagtgtacct
      721 gaataatgac cccactcgtc ggacttatct tacgtatttt atccatagtc atgttgtgtg
      781 tctttttagg catcaccatt tggcttttat ggcgcgcggt atccgccaaa gaagccaccg
      841 ccggtcggct tgccatcccc accatttcac tggtcagcga cgaagacggt gttcgtgaag
      901 aactgacctt caacagtcag gaagtcacca tcgggcgcag tcccgaatgt gattttacac
      961 tcaataacca caccgtttca gcccggcatg ggcgtctcaa tttccatctc aatcaatggt
     1021 ggtatgaaga cctgaaatca accaacggct cgttcttgca aggcatgcgc attgaagaac
     1081 cgattgtcat caaagatggt gacgaaatcg cttttggtga cgtttacgtg agtgtgttta
     1141 ttaaagcagt cgataatata aagcagaggt aatgatgaca gaagaaattt tattaggcgt
     1201 aataggtggc tcagggcttt atagctttga agaactcgaa aatcaggaaa gtattgaaat
     1261 cgacactcct tttggcaaac cctcatcgcc gattgtcatc ggcgagataa agggtcagaa
     1321 aatcgccttt ctcgcgcgcc atggcatcgg gcatgtttat tcgcccacgg atgtcaacta
     1381 ccgcgccaac atctacgctt tcaaatccct gggcattcgc gctatcgtca gcgtcagcgc
     1441 ggttggctct ttgcgtgaag attacgcgcc cggacacatc atcgtccccg accagattta
     1501 cgacaacacc aaagaccgca aacgcagctt ttttggcgag ggtatggtcg tgcatatcag
     1561 ttcggcagac cctttttgcc ctgctttaag tcaacatctg cttaaagcct tgcgcgaaca
     1621 agacgccatc gtgcatgaag gtggcacgct gattacgatt gaaggcccgc gctttagcac
     1681 acgcggcgaa tccgaaacct tccgcaaatg gggtatgtcc ctggtgggca tgaccgccgc
     1741 gccggaagcc tttttggcac gcgaagccga gatgtgctac gccacgatgg cgcacgttac
     1801 cgattttgac tgctggcatg tcagcgaaga agctgtcact gtggaaatgg tggtaaacac
     1861 gctgcagaaa aacacccgaa ccgcgcaaaa aagcatcgtg cggttggtgg agttgcttgc
     1921 cgaagcgcaa agcgcaggca aagtcagcta ctgcgaatgt gaacacgcgc ttaaagacgc
     1981 catcatgacc aaccctaacg ccatttgcga agataaaaag caaacgcttg aactgctgac
     2041 gggaaaatac tttaagtaga cttctatgaa caacgacctt aaagcaccgc gacaacccag
     2101 gttgattctc tttgggattt tgtttgtgtt tttttacaca gcggcgcttt cgctggcgag
     2161 tgcgattcga acacacgcgc caggttttca ggttgactgg tattacttta tcccctttgc
     2221 agcctggctc ataggcgcgc tgggcttaga ttacgcggtt aagcgctttt tgcccaaccg
     2281 cgacccctgg atattccccg ttgttatgct cttgtgtggc tggggactgt tgaccatttg
     2341 gcgtctttcc cctaccctgg gacaaaggca gattatctgg tttctcttgg catgtctcct
     2401 tttttacttc ggcattcgcc aaaaagaact ggtcagcaaa ctaaaaggcc taaaatactt
     2461 atggcttctg tttggattgc tccttatcgg gctcactttt tggattggtg tcaaccccag
     2521 tggcagtggc cctaccagat ggcttagtgc ctttggtatt ttctttcaac catcagaacc
     2581 attaaaactc ttcctgctgg tttacatcgc ggcttatttc gccgatcagg taaaacccaa
     2641 cacatcactc atcgccagca tcttacccac gcttatcatc atcgctgtca ttggcgtgat
     2701 gctcatcagc cagcgtgatc tgggcaccgc cgccttattc ctgggctttt atgtgctcat
     2761 gctcacggtt agcagcaaca gtcgcaaata cctgtggatt gtgcccgtgt tggccatcat
     2821 agctattgtt gttggttatt ttacgcttga catcgtcaaa acccgcgtgg atatttggct
     2881 caatccctgg ctgaatacat cgggcagttc ctaccaaatc gcgcaggcgc aaattgccgt
     2941 cgcgtccggt ggtctatatg gaaccggacc gggcttaggc agtccgggtt ttgtaccggt
     3001 ggcggtatcg gactttattt tcacagccat cgccgaagaa accgggctta tcggcacatc
     3061 agccttgctc attctcttcg cgattgtcat tatccggggc attaatatcg cgctggcttc
     3121 caaaacaacc tacggccgtt atctcgcctt tggcatctcc atgttcctga ccttgcaatg
     3181 tctttggatt atttgcggta atttgggctt gctcccgctc actggcgtaa ccttgccatt
     3241 tatgtcctat gggggctcat cgctggttac cagcatctta gctatcttgt tattgctaaa
     3301 aatcagtgcc gattcaagta ccattccctt gcctgataaa gcgcgcaaac cctatcatcg
     3361 catcgccgcg atagtgcttg gtatctttgt gctcatcatc ggctggaaca gctacctggc
     3421 gatattcaac caggacgcca tcgttaacaa agcggaaaac atgcgctggg cgattgatga
     3481 ccgctattcg ccattaggca atatcagcga ccaaagtggt aatcccatcg tgcgcacgat
     3541 cggcacgcca ggcgactatg aacgtgaatt gctcgtgcct ggactaagta taaccatcgg
     3601 ctacacaaac cccgtcttgg ggaaatcagg gcttgaacgt agcctgtacc cctatttgcg
     3661 tggcacagag acgcgcgacg aaaccttaag cagtcaatac cagttgcttt ataatcagcc
     3721 cccgccaggc agtgatgttt ctgtgaacat tattctcgac ctgcaagcca aagcggataa
     3781 attgcttggc gataagcatg gcgccattat cttgatgaac gccgtaaacg gcgaaattta
     3841 cgctatggtc tcccacccct actttgacta tacaagtatc gctgaagact gggatagctg
     3901 gcgccaacgc gaagacgcgc cccttctcaa ccgcgttacc caaggtgtct acccgcttgg
     3961 cacgctggca aatggtttcg ccttaagcag ctactgggtg caggaaggca ccaacacgct
     4021 cagtattcca ctccacagtg atgcaatcga cccattatgt gggaaaatgc ttactgtcac
     4081 cgaccaggtg aatcccatcc agcacggttg ccaggaagct acacgcgatt taatcaacgc
     4141 taatgaccca ctggctctaa ccgaacaact cgacaagttt ggtttctaca gcatacccag
     4201 tttctccctg gaacttgctc agcccacaag tcgaccgggt aaaaactact ttaataatat
     4261 ccaaacgctc atacctgcca cccagctcag ccccctgcaa atggcactca tcgccgccag
     4321 catcaccaac gatggagaca aaatcacccc gcgtctggtc aacgcctatc aaactgaaaa
     4381 tggcagctgg aaaagctaca cagaaccatc acaggcaatg cgtgttttgc cccagaccgt
     4441 cgccaaaacc actcgcgatt atttcagcca gcataacaaa gcctactggt atatgatggg
     4501 tcacagcgcg atggaagaag aacagacgat tgtttggtac gttgggggaa caaacgacgc
     4561 ctggaccggc acgccaatgg tgatcgtcat cgcgcttgaa gacagtcagc ctgacctggc
     4621 ttatcgcata gctgaccagc tcttcgtgcc acaatctgaa gattagttaa tcgaagcacc
     4681 gttccatatc cacaattaaa caggaaaaga aaaggctaca gcacaaagcc atagccttca
     4741 tttttgaacc aaaaacagtt attcttatat aagaatccgc acgaacttgc gtttgccaac
     4801 ctgcaaaacg ccacccgtgt caagcaccgc ggttgggtca cccacagtga caccgtccag
     4861 tttcacaccc tgttgggaaa tgacacgacg tccttcactg cggctggaca ccaggccctg
     4921 atcaaccaaa acgtccaaaa ccgtatgttt gccagcttcc aaaagaactt cag
//
LOCUS       DAOSFC010000087         4945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_15179_length_4945_cov_4.611452, whole genome shotgun sequence.
ACCESSION   DAOSFC010000087 DAOSFC010000000
VERSION     DAOSFC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15179_length_4945_cov_4.611452"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PK791_03250"
     CDS             complement(<1..336)
                     /locus_tag="PK791_03250"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOH92363.1"
                     /translation="MTDSEKLKLLEALLFSSPGLASVDALAQAMDISKKECAQLLETL
                     KTQLKENHGIRLQTVKNQYQLVTAAEYAPAIESFLGLEVTTRLSQAALEALAIIAYKQ
                     PTTRPEIDSI"
     gene            454..1083
                     /gene="rsmD"
                     /locus_tag="PK791_03255"
     CDS             454..1083
                     /gene="rsmD"
                     /locus_tag="PK791_03255"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOH92364.1"
                     /translation="MATLRVISGSAKGKRLKTVPGDSTRPITDQVKEALFNILADEVL
                     DIDILDLFGGTGAVGIEALSRGANSVLFLDTSHQAIKVIKDNLQTTKFSQQATVIRGD
                     AFAYIQNPSRLGYDLIYVAPPQYKDMWEKAMRLLDENPDLLHDDGQIIVQINPIEWSE
                     QNYENFIEFDRRKYGDTELIFFEKPYDDLDDEDLSDDAESESNENDICN"
     gene            complement(1008..1562)
                     /locus_tag="PK791_03260"
     CDS             complement(1008..1562)
                     /locus_tag="PK791_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOH92365.1"
                     /translation="MGVEKQGADSKRYTLIPRVLVFAVADSGQVLLLQGALDKKIWPN
                     IWNGIGGHVEQGESVLQATHRELLEETGLTAQIMQYCGQVVVDTGTKPGIIFFAFKAK
                     ALSGQQRESAEGRLAWFTSEQAMRLDLVEDLYTLLPMVLGQEPNVEPFWGLYSYDETG
                     QLQMSFSFDSDSASSLKSSSSKSS"
     gene            complement(1565..2740)
                     /locus_tag="PK791_03265"
     CDS             complement(1565..2740)
                     /locus_tag="PK791_03265"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOH92366.1"
                     /translation="MAKKRIGILTGGGDVPGLNIAIKSVVLNAVEDDYEVIGIRRGWG
                     GLLNYEIGNQESHDLYIRPLTPLAVRRVDRTGGTFLHTSRTNPSHVRPKDVPEFLKNS
                     SKGKLSNEENGAMDYTDHILEVLDHLGIESLITIGGDDTLSYSVQLHKTGFPLVGIPK
                     TMDNDVLGTDYCIGFSTAVTQAVSLITNFRTSVGSHERIGVVELFGRNSGETSLITGY
                     LSYVDRTVICEVPFNFVELSEMLAKDKRDSPSHYSICVISEGAVTDSGLIVESGEEDA
                     YGHKKLGGIGDLLSEQIKILTGNNTMYQKLGYLLRSGPADMLDRMVAMNYGALAMQMT
                     KRKDFGNMVSIMNGNYSEVPLETVTSGKRHVDVDLYYDAANYRPKIKNILGLPMFLH"
     gene            complement(2733..3971)
                     /locus_tag="PK791_03270"
     CDS             complement(2733..3971)
                     /locus_tag="PK791_03270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOH92367.1"
                     /translation="MKKTANIWMPIVILIVLVGATFLNLELSERYGFEEGFITRYAAA
                     KNWMKTGVSPYSEESHALALELAGDENALAGNLDQGRFLDPVPYVLFYIPLSFLPYSI
                     AKAIWMTIIEVAIVLSAWLAMKIVALKLSIPETILISIGLLFFYPNLKMILSASVLPL
                     YFFFIIWAIWLAMNKQGRMAGVLFFLAFAMVPISLFIAIFFMIWLGARRDNSLTTIFF
                     VGIAFLILIGLILFPNWLAEWFTAYVLVYPKFTWLDSPIMRISELFPGAMRQLAIGLN
                     IATVVYLFVEWYGLPSREQRGLQWKLSLTMVLLYFLNPLKAVSYQILLFIPLFAIYKY
                     LNEKWRVGGRIVTWLSFLSIGIIYWIKGTAQETFVSGGADLIILLLPMISFLGLQYFR
                     WWALKSSSALIESTNGGYYG"
     gene            4194..>4945
                     /locus_tag="PK791_03275"
     CDS             4194..>4945
                     /locus_tag="PK791_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH92368.1"
                     /translation="MARITKTSGINEDPVTRTASRLIELGRKKGQVTYDQIMNYLQEA
                     EIEPDQFDEIVLSLQNAGITYVEDSHPEDDPSDTELSDTEEFGEEPNDQELADIASEG
                     DDLANIDTDDTIGLYLKEVSRVDLLNSIEEEVALAKRIEKGREAREELAKGTHSMKKL
                     LQLQADIEDGDQAVDHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRATKKFE
                     YQRGHKFSTYATWWIRQAITRAIADQGRTIRVP"
BASE COUNT         1464 a         1231 c         1035 g         1215 t
ORIGIN      
        1 aatgctgtca atctccggac gcgtagtagg ctgtttataa gcgataatcg ccaatgcctc
       61 tagtgccgcc tggctcaatc gtgtggtgac ttccagacct aaaaagcttt ctattgcagg
      121 cgcgtactcc gcggcagtca ccagttgata ctgatttttc accgtttgta agcgaatgcc
      181 atggttttct ttcaattgcg tttttagcgt ttcaagcagt tgagcgcatt ccttcttaga
      241 aatatccatc gcttgcgcca aagcatccac gctggccagg ccaggcgaac taaacaataa
      301 agcttcaagc aactttaatt tttcactgtc tgtcatattt tctcagctct ttgcccgtgc
      361 tataatgctg gtattcacgg atgctgtgat tatactccat ggcatctcaa acatgaaaga
      421 atattaaaca atgcgcggaa aggtgctaaa cgaatggcaa cattaagggt aatatctggc
      481 agtgccaaag gcaaaaggct caaaactgtg cctggcgatt ccacgcgtcc catcaccgac
      541 caggtaaaag aagcgctctt taacattttg gccgacgaag tgcttgatat tgacatactc
      601 gatcttttcg gtggcacggg cgcggttggc attgaagccc tcagccgtgg cgcgaactct
      661 gtccttttct tggataccag ccatcaagcc atcaaggtca tcaaagataa tcttcaaacc
      721 acaaaattta gccaacaagc caccgtcata cgtggcgacg cttttgccta cattcaaaat
      781 cccagccggc ttggctatga cctcatctat gtcgcgccac cccaatataa agacatgtgg
      841 gaaaaggcga tgcgcctgtt agacgaaaac ccagaccttt tgcacgacga cgggcagatt
      901 atcgtgcaaa tcaatcccat tgaatggtct gaacaaaatt acgaaaactt tattgagttt
      961 gaccgccgaa aatatggcga tacagaattg attttctttg aaaaacctta tgatgattta
     1021 gatgatgaag acttgagtga tgacgctgag tctgagtcaa acgaaaacga catttgcaac
     1081 tgacctgttt catcataaga gtatagcccc caaaatggtt caacgttagg ctcctggccc
     1141 aaaaccatcg gcagaagcgt ataaagatct tccaccaagt ccaaacgcat cgcctgttct
     1201 gacgtaaacc acgccagtcg tccttccgcg gattcacgtt gctgaccact caaggctttg
     1261 gctttgaacg caaaaaagat tattccaggt tttgtgcccg tatccacaac gacctgtccg
     1321 caatactgca tgatttgggc tgttaagccc gtttcttcca ggagttcgcg atgggtggcc
     1381 tgcaaaaccg attcaccctg ctccacatgc ccgcctatgc cattccaaat gtttggccaa
     1441 atttttttat ccagcgcgcc ttgcaaaagc aaaacctgac ctgaatcagc gacagcaaac
     1501 accaacaccc tgggaatcag tgtgtagcgt ttgctgtcgg caccttgttt ttcaacgccc
     1561 atttttagtg caggaacatg ggcaatccaa gaatgttctt aatttttggt cgatagttcg
     1621 cagcgtcata atacaaatca acatccacgt gtcgcttacc actggtgacc gtttcaagcg
     1681 ggacttcgct gtagtttcca ttcataatgg ataccatgtt gccaaagtct tttcgtttgg
     1741 tcatttgcat cgccagcgcg ccatagttca ttgccaccat gcggtcaagc atgtccgctg
     1801 ggccactgcg caagagatag cccagtttct ggtacatggt gttattgcct gtcaaaattt
     1861 taatctgctc tgaaagcaaa tcaccaatac cgcccagttt tttgtgacca taggcatctt
     1921 cttcgccgga ttcaacaatc aaaccactgt cggttaccgc gccttcagag ataacacaaa
     1981 tggaatagtg ggatgggctg tcccgtttgt cctttgccag catttcgctc agttcaacga
     2041 agttaaaagg cacttcgcag ataaccgttc ggtcaacata gctcaaatag ccggtgatca
     2101 aacttgtttc acccgagttg cggccaaaaa gctccaccac accgatgcgt tcatgcgaac
     2161 caaccgacgt gcggaaattc gtaatcagcg aaaccgcctg ggtaaccgcg gtcgaaaagc
     2221 cgatacagta atccgtgcct aaaacgtcgt tgtccatcgt ctttggaata cctaccaagg
     2281 ggaatcctgt tttatgcagt tgcacagaat agcttagtgt gtcgtcccca ccaatcgtaa
     2341 tcaaagattc aatgccaaga tggtccagaa cctcaaggat gtggtctgta taatccatcg
     2401 cgccattttc ttcgtttgat aattttcctt ttgatgagtt cttgagaaac tcaggcacat
     2461 cctttggacg aacgtgtgaa gggtttgtgc gtgaagtgtg caggaaagtg ccaccggtgc
     2521 ggtcaacacg cctgacagca agtggtgtca gtggtcggat atataaatcg tgcgattcct
     2581 ggttgccgat ttcataattt aaaagtccgc cccaaccgcg acggatgccg ataacttcat
     2641 agtcgtcctc gacagcgttc aaaaccaccg atttgatggc aatattcaag ccaggcacat
     2701 cgcccccgcc tgttagaata cctattcgtt ttttagccat aataacctcc attagtcgat
     2761 tcaataagcg cgcttgaact ttttagcgcc caccatctaa agtattgcaa gccaagaaaa
     2821 ctaatcatcg gcaaaagcag aataattaaa tccgcgccac cagatacaaa agtctcttgt
     2881 gctgttccct taatccaata gatgatgcca atgctcagaa agctcagcca agttactatt
     2941 ctaccaccca ctcgccattt ttcgttcaaa tacttataaa ttgcgaaaag tggaataaat
     3001 aacaagattt gataactgac tgccttcagt gggtttagga aatagagtaa aaccatggtc
     3061 aaactcaatt tccactgcaa gccacgttgt tcgcgcgacg gcaaaccata ccactcaaca
     3121 aaaagataga caaccgtggc gatgttcaaa ccaatcgcca gttgacgcat tgcccctgga
     3181 aacaactcac tgatacgcat aataggcgag tcgagccagg tgaactttgg atagaccaaa
     3241 acataagccg taaaccactc tgccagccag ttgggaaaaa gaattaagcc aataagtatt
     3301 aaaaaagcaa tccccacaaa aaagatggtg gtcaaagaat tatcacgccg ggcacccagc
     3361 caaatcataa agaaaatggc aataaaaagg ctgataggca ccatcgcaaa ggctaaaaag
     3421 aataaaacac ccgccatacg cccttgtttg ttcattgcca gccaaatcgc ccaaatgatg
     3481 aaaaagaagt aaagcggtaa aacgctggcg gaaagaatca ttttcaggtt tggataaaag
     3541 aaaagcaaac caatacttat caaaattgtt tctggaatgc tcaatttcaa agcgacaatc
     3601 ttcatcgcca gccaggcact aagcacaatc gcaacttcga taatcgtcat ccaaatggct
     3661 ttcgcgattg aatagggcaa aaaactaagc gggatataaa aaagcacgta agggaccggg
     3721 tccaaaaaac gcccctggtc caaattgccc gccaacgcgt tttcatctcc tgctagttca
     3781 agcgctaagg catggctttc ttcactgtaa ggcgaaaccc ccgttttcat ccagttctta
     3841 gccgcggcgt aacgggttat gaaaccttct tcgaaaccat agcgttctga taactcaagg
     3901 ttaagaaaag tcgcgccaac cagcacgatt agaataacaa tgggcatcca aatatttgcc
     3961 gtctttttca aaaacacgta tgaacctctc ttatcaaaac gattctatca gcaaggttta
     4021 aggtggtcaa acaaaacccc cgcgcttgcc aacaaaccgt accaactcac catcatatct
     4081 acgcgcagat aaaatgatta tcagctgatt tccactaaaa acttgagttt catctgaaaa
     4141 tattgtactc tatgatggtg tgaataattt tgacagaaaa ggtagcattt tgaatggcaa
     4201 gaattacaaa aacatctggt attaatgaag atcccgttac aagaacagct agtcgtttga
     4261 ttgaattagg acgtaaaaaa ggacaagtta cgtacgacca aatcatgaac tatttacagg
     4321 aagctgaaat agaacctgat cagtttgatg aaattgtact cagtttgcaa aacgctggca
     4381 tcacttacgt tgaagacagt catcccgaag atgacccaag cgacacggaa ctttctgata
     4441 ccgaagaatt tggcgaagaa cctaatgacc aggaactggc cgatatcgct tccgaagggg
     4501 atgaccttgc caacattgat actgacgaca ctatcgggct atatctcaaa gaagttagcc
     4561 gcgtcgattt gctcaacagt atcgaagaag aagttgccct tgccaaacgt atcgaaaaag
     4621 gtcgcgaagc ccgcgaagaa ttagccaaag gcacgcatag catgaaaaaa ctcctgcagc
     4681 ttcaagccga tatcgaagac ggcgaccaag ctgtggacca tctcatcacc gccaactcgc
     4741 gcctggtgat cagtgtagcc aaaaagtaca tgggacgtgg cgtgccattc ctggatctca
     4801 ttcaggaagg caatatcggc ctcattcgcg ccaccaaaaa atttgaatat caacgcgggc
     4861 acaaattcag cacctacgcc acctggtgga ttcgccaggc cattacgcgc gcgattgctg
     4921 atcagggccg caccattcgc gtccc
//
LOCUS       DAOSFC010000088         4927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_15264_length_4927_cov_5.461412, whole genome shotgun sequence.
ACCESSION   DAOSFC010000088 DAOSFC010000000
VERSION     DAOSFC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15264_length_4927_cov_5.461412"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1743)
                     /gene="dnaE"
                     /locus_tag="PK791_03280"
     CDS             complement(<1..1743)
                     /gene="dnaE"
                     /locus_tag="PK791_03280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOH92369.1"
                     /translation="MSFVHLHVHSSYSILDGFGKPADLVARAKELGMPALALTDHGTM
                     FGTLDFYKAAKNAGIKPIIGLETYVAQRRMQDKDPQKDRHSYHLILLAKNKTGYQNLL
                     KLASISQLEGHYYTPRIDKIALAEHSEGLIASSACMSGEVSRALLDNDHHRAERVVAW
                     YREVFGRDNYYLELQDHDIPDQYRVNRLMLELARKTDTRLIATNDVHYIRPEDAALQD
                     ILLCIQTGKLLSDPVRMHMSDNSFYMRSPEEMKLLFSQVPDAIENTLEIAERCHLDLG
                     RKGYHLPQFEVPAGETTSSYLRKLCEDGLRRREPDRADSPEMKARLDYELGVIEQMGF
                     DAYFLIVWDLCRYSREKKIWYNVRGSGNGSLVAYALEITSVEPLSHKLLFERFLNPDR
                     VTMPDIDLDYQDDRRAEVMEYCNQKYGASHVAQIITFGTMAARGAVRDVGRVMNIPLP
                     DVDRVAKLVPSAYQGKAIPLADSLEKVSELKEVYNSSEEMKKLLDTASAIEGAIRNVG
                     THAAGVIISDKPLTEYVPLHRPTNVNENLPIKTVAQYDMDGITELGLLKVDFLGLVTL
                     TIMAKACEYIKARGG"
     gene            complement(1903..2829)
                     /locus_tag="PK791_03285"
     CDS             complement(1903..2829)
                     /locus_tag="PK791_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92370.1"
                     /translation="MSSFRDLRIVDNFYQTSAFFPMPTVLIGTLTEDGKTSLGPYSLV
                     QPYYVAGKDYYAMLLCARNNSNTAQHLLARGKCSINFITDEKKWFKEAVKLSWPGDTP
                     EEKMKNCQFTLEDGLMAKAHPEQVFPKVVTQSFQVFECTWMSDLENAFEDKPGQLDGY
                     PPPYRNFNGITSQFGAHFILRVDKILMKEKQYQGIVNGVKAKDFPRVPVDYGYRDSKN
                     FWYTRFSRPIPEILPMRKGTLQSVRYAADRIDPDIGFDDAALEKLLNVPRVFLNTVLK
                     GCVSWAKENDVSYITAEHMDIINDKRAKEKQR"
     gene            complement(2831..3826)
                     /locus_tag="PK791_03290"
     CDS             complement(2831..3826)
                     /locus_tag="PK791_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH92371.1"
                     /translation="MTRLVIQIPAYNEAKTLPIALAELPRQIEGVDDIKVVVIDDGST
                     DNTAQIALENGADYVLRHRQNRGLSRAFMDGIQFSLALGADVIVNTDADNQYPGTDIP
                     DLIKPILDKTHDLVIGDRQLDKNVHFSAFKRLLERLGSAFVRRISSTIVPDAASGFRA
                     FSRYAALPLQVYNPYSYTLDTLIQAGKSRLNITSVPIHTNPATRESRLHKGMAHFIWR
                     QGGAIIRSYLLYQPLRSFLISGLVVGLPGLALVIRFLVYYLMGDGGRFVQSVTLGGSM
                     IVASLLLTIFGFLADATRANRQLLEENLIRQRDAFRYNPNEKQEFLGLDVLHKEK"
     gene            complement(3839..4066)
                     /locus_tag="PK791_03295"
     CDS             complement(3839..4066)
                     /locus_tag="PK791_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOH92372.1"
                     /translation="MLADIQIGDLVRMRKQHPCGGSTWVVTRIGADIGLECLTCQRRV
                     MLTRRELNRRAKTITAGTQNNALGPTSPSQE"
     gene            4201..4845
                     /locus_tag="PK791_03300"
     CDS             4201..4845
                     /locus_tag="PK791_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOH92373.1"
                     /translation="MFGLDLPTLINRVIILVIAFTVHEYMHAFVAYKFGDWTAKNAGR
                     LTLNPLVHLDPLGSIMLLFAGFGWAKPVPVNPYVIRQKHPAGLMLVSLAGPLSNLVLA
                     AVGGGIIRLLVVVRYTWMAPEWLVNFLLEFIVINIMLFLFNMIPIAPLDGDKIFEYTL
                     PEAWKSKFETVRQYGSYILFGMMIIGRFTKFDVFGFLMDKPMSHIFSLLTGFRV"
BASE COUNT         1174 a         1085 c         1339 g         1329 t
ORIGIN      
        1 gccaccacgg gctttaatgt attcgcaagc ttttgccatg atggttaagg taaccaagcc
       61 caaaaagtca actttcagca agccaagctc ggtgatgccg tccatgtcgt actgggcgac
      121 agttttgatg ggcagattct cgttcacgtt ggtcgggcgg tgcaggggaa cgtattcggt
      181 taagggtttg tcgctgatga tgacacccgc cgcgtgtgtg ccaacgttgc ggattgcgcc
      241 ttcaatggca ctggctgtgt cgagcagctt tttcatctct tcggagctgt tgtaaacttc
      301 tttcagctcg gatacttttt ccagggaatc tgccaggggg atggctttgc cctgataggc
      361 tgaaggtacc agctttgcca cacggtccac gtcaggcaat gggatgttca tcacacgacc
      421 cacgtctctc accgcgccgc gggcggccat tgtgccaaaa gtgataatct gagccacatg
      481 gctggcaccg tatttttggt tgcaatattc cataacttca gcgcgacggt cgtcctggta
      541 gtcaaggtcg atatcgggca tggtaacgcg gtcggggttc agaaaacgtt caaagagaag
      601 cttgtgtgac aagggttcaa cagacgtaat ttcgagcgcg taggcaacca gggaaccgtt
      661 acccgagcca cgcacgttat accaaatttt cttttcgcgg gagtagcgac acaaatccca
      721 gacgattaag aagtaggcgt caaaacccat ttgttcaatc acgcccagtt catagtcaag
      781 gcgggctttc atctcgggtg agtcagcgcg gtcaggttca cgtcgtctga gcccgtcttc
      841 gcacaattta cgcagataag agcttgtggt ttcgccagcg gggacctcaa actggggcag
      901 atgatagccc ttgcgaccaa ggtcaagatg acatcgttcc gcgatttcga gcgtgttttc
      961 gattgcgtcg gggacttgcg aaaagagcaa tttcatctct tcgggactac gcatatagaa
     1021 cgagttatcc gacatatgca tgcgcacggg gtcacttagc agtttgccgg tttggatgca
     1081 tagcagaata tcctgaaggg cggcgtcttc ggggcggatg tagtgcacat cgttggtggc
     1141 aatcaggcgc gtgtccgtct tgcgggcaag ttccagcatc aggcggttaa cacggtattg
     1201 gtcggggatg tcatggtcct ggagttcgag ataataatta tctctgccaa agacttcacg
     1261 ataccaggcg acaacgcgtt cagcgcggtg gtggtcgtta tcgagcagcg cgcgggaaac
     1321 ttcgccagac atacaggcgg atgatgcaat cagcccttcg ctgtgttcag ccagggcgat
     1381 tttatcgatg cgcggtgtgt agtaatgccc ctcaagctga gaaatggagg caagttttag
     1441 caggttttgg taaccggttt tgtttttggc aagcaagatc agatggtagg aatgacgatc
     1501 tttttgaggg tctttgtctt gcattcggcg ttgggcaacg taggtttcca gaccgataat
     1561 gggtttgatg ccggcatttt tagctgcctt gtaaaagtcg agcgtgccga acatggtgcc
     1621 atggtcggta agagccaaag ccggcatgcc aagttccttg gcgcgcgcca ccaaatcggc
     1681 tggtttgccg aatccatcca ggatggaata tgaagaatgg acgtgaaggt ggacaaagga
     1741 catagtttgt ttctaaggta tttctgtttg ttttacggcg cttgagaaac tttaaaaaac
     1801 cgaaaaccgt tttagcatta cacacataag tatagcacag cgtgtctgcg attcctggct
     1861 tgaagcctta aaagtcttga tattttaagg ttaaagaaag gtctagcgtt gtttttcctt
     1921 tgcgcgtttg tcgttgatga tgtccatgtg ttcagcagtg atgtagctga catcgttctc
     1981 ttttgcccaa ctaacacagc ccttaagaac ggtgttgaga aagactctgg gcacgttgag
     2041 aagcttttca agggctgcat catcaaaacc gatatccggg tcgatgcggt cggcggcata
     2101 gcgcactgat tgaagcgtac ctttgcgcat aggcaatatt tcggggatgg gtctgctaaa
     2161 tcttgtgtac caaaagtttt tggagtcacg atagccataa tcgactggca cacgtggaaa
     2221 gtctttggct ttcacgccgt tgacaatccc ctggtattgc ttttctttca tcaggatttt
     2281 gtcgacgcgc agaatgaaat gggcaccaaa ctgactcgtg atgccattga aattgcgata
     2341 gggtggcgga tatccatcca gttgcccggg tttgtcttca aaagcgtttt ctaaatcact
     2401 catccaggta cattcaaaaa cctgaaagct ttgcgtgacg acttttggaa agacttgttc
     2461 ggggtgggct tttgccatca gcccgtcttc aagcgtaaac tggcagtttt tcattttttc
     2521 ttcgggtgta tcgcctggcc aggataactt gacggcttct ttgaaccatt ttttctcatc
     2581 agtgatgaaa ttgatcgagc atttaccacg tgctaaaagg tgttgcgcgg tgtttgagtt
     2641 gttgcgggcg caaagaagca tggcgtaata gtcttttccg gcaacataat agggttgaac
     2701 aagtgagtag ggtcccaaac tggttttccc atcttcagtt aaggtgccga tgagaacggt
     2761 tggcattggg aaaaaggcgg aagtttgata aaaattgtca acgatgcgta aatccctaaa
     2821 gctactcaat ttatttctcc ttatgcagaa catccaggcc taaaaattcc tgtttttcat
     2881 tcggattgta gcgaaaagcg tcgcgctggc ggatgaggtt ttcttccagc aactggcgat
     2941 tggcgcgggt agcatctgcc aggaagccaa aaatagttaa aagcaaactg gcaacaatca
     3001 tcgagccacc caaggtaaca ctttgaacaa atcgaccacc atcgcccatg aggtaataaa
     3061 ccaaaaaacg gataacaagc gccagtccgg gtaaaccaac cacaagcccg ctaatcaaaa
     3121 agcttcgcag gggctggtaa aggagataag agcggataat agccccaccc tgacgccaaa
     3181 tgaaatgtgc catgcctttg tgcaagcgtg attcccgggt ggcaggattg gtatgaatgg
     3241 gaacgctggt gatattcagg cgtgatttgc cagcctggat gagtgtgtca agcgtgtagg
     3301 aataagggtt gtaaacctgc aaaggcaatg ccgcgtagcg cgaaaacgcg cgaaagccac
     3361 tggcagcatc aggcacgatt gtgctggaaa tgcgtcggac aaatgcgctg ccaagtcgtt
     3421 caagcaggcg cttgaaagcc gagaaatgga cgtttttatc cagttgtcgg tcgccgataa
     3481 caaggtcgtg ggttttgtcc agaatcggtt tgataaggtc aggaatatcc gtgccggggt
     3541 actggttatc ggcgtcggtg ttgacgatga catccgcgcc cagtgccagc gagaactgaa
     3601 tgccgtccat aaaggcgcgt gaaagaccac ggttttgacg atggcgcagg acgtaatcgg
     3661 caccgttctc aagagcgatt tgggcggtgt tatctgttga gccatcatca atgaccacaa
     3721 ctttgatatc gtcgacgcct tcaatctggc gcggaagttc tgccagggca attggcaagg
     3781 ttttagcttc gttgtaggct gggatttgaa tgacaagtct ggtcatgggc tttcctttct
     3841 attcctgtga tggcgatgtt gggcctaacg cgttgttttg tgtacctgcg gtaatggttt
     3901 tggcgcgcct gtttagttcg cgccgtgtca acataacccg tcgctggcag gtcaggcatt
     3961 cgagaccgat atcggcaccg atacgggtaa caacccaggt tgacccaccg caagggtgct
     4021 gtttgcgcat gcgcacaaga tctccgattt gaatatcggc taacatggtg agattatatc
     4081 actgcgtgat acaatttcag ggaattatta aataagagcc agtaaacctt aatctgacag
     4141 aatgtttgag ccatgaaagg tttgtttttg gcaggctaag agtcattgaa ttggagttat
     4201 atgtttggac ttgatcttcc gacactgatt aaccgcgtta ttattttggt gatagctttc
     4261 actgttcatg agtacatgca tgcttttgtg gcttataaat ttggcgactg gactgcgaaa
     4321 aacgcagggc gcttgaccct gaaccccctg gtgcacctgg acccgcttgg ttcaatcatg
     4381 cttttgttcg ctggttttgg ctgggcaaag ccggtgccag tgaatcctta tgtcattcgt
     4441 caaaaacatc cggcaggttt gatgctggta tcgctggctg gacccctgag taacctggtt
     4501 ttggcagccg tgggtggtgg aataatacgc ttgttggtcg ttgtcaggta cacctggatg
     4561 gcgccagagt ggctggtaaa tttcctgctt gagtttatcg tcatcaatat catgcttttc
     4621 ctgttcaata tgattcccat tgccccactg gacggggata aaatctttga atataccctg
     4681 cctgaagcct ggaaaagtaa atttgaaaca gttcgtcaat atggctcgta tattttgttt
     4741 gggatgatga taataggtcg gttcacgaaa tttgacgttt ttggtttttt gatggataag
     4801 ccgatgagcc atattttttc ccttctgaca ggttttaggg tctgatgcgt ttctattgct
     4861 acatggtgga atgcgctaac ggcgcgtttt acacaggttg gtcgaacgac ccagttcgac
     4921 gagagaa
//
LOCUS       DAOSFC010000089         4900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_15378_length_4900_cov_5.394634, whole genome shotgun sequence.
ACCESSION   DAOSFC010000089 DAOSFC010000000
VERSION     DAOSFC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4900
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15378_length_4900_cov_5.394634"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PK791_03305"
     CDS             complement(<1..893)
                     /locus_tag="PK791_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOH92374.1"
                     /translation="MARDILSARQDFPILDIQVNDRPLVYLDNAATTQKPQVVIDTLR
                     DYYTRYNANIHRGNYAIAYEATEAYEKARAKVAGYINAEHKHEIIFTRGTTESINLVA
                     YSFGERFVGEGDEIIVTQLEHHSNYVPWKLLCERKKATFRVVPFDENGDLDLEVYKSL
                     LNEKTRVVAVNHVSNSLGTINPVKEIIQLAHNAGAVVLVDAAQAVQHIEHDVQALDAD
                     FYAFSGHKMYGPMGVGVLYGKEKWLDAMPPFLSGGEMISNVTPEKISFNVLPYKFEAG
                     TPNVPGPIGLAAAIDYLQEFD"
     gene            complement(957..1967)
                     /locus_tag="PK791_03310"
     CDS             complement(957..1967)
                     /locus_tag="PK791_03310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH92375.1"
                     /translation="MHYNLTMSTKANPIQFSVLILHWRAEQYLQTCLEALDAQTYKNF
                     EVLLLDNGNEIPLAPDFADDFANLSVKVLRSEKNLGFAAGNNYLAGFVRGRYLVLLNA
                     DAFPDPDWLANINEALQANPNHFFASRLIKYLEPDKLDGEWHVYHASGLAWRHNHNQA
                     IIHASDEPKEVFGACAAASVYPREAFDAVGGFDEDFFAYMEDIDLDFRLQLAGYPCLY
                     LPNAVVKHVGSASTAPRSDFAVYHGHRNLPWVFIKNMPGWLFWLLLPSHFLVNLAYLL
                     LAPFIGKAKVMAKAKWDSLKGLKTIIAKRRLVQKNRRVSVMHIAKLLNWNVFAPLIKR
                     KF"
     gene            2049..2915
                     /locus_tag="PK791_03315"
     CDS             2049..2915
                     /locus_tag="PK791_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH92376.1"
                     /translation="MVRFSVVNTRTVSEKIKPLYDSSKRVSPAIAELKEIGNYRHLLA
                     QLIRRDILTRYKRSFLGVAWTMLNPLGTMLVMTIVFSNIFRASMPGVPFPVYILSGLV
                     VWNFFSQGTTASMSGLVWGGNLLQRIFVPRTIFAVSAVGTALVNTVIALVPLIIVMLI
                     TQATFSWALLFLPIAIILVAFFTLGFGLLISSLAAFFPDVMEMYQIVLTAWMYLTPII
                     YPESIIPKHLFNVYRLNPMYWLVKIFRAPIVDQSLPALSDFGIAAAISIIMLVIGWLV
                     FTSKSDEYAYRI"
     gene            2899..3645
                     /locus_tag="PK791_03320"
     CDS             2899..3645
                     /locus_tag="PK791_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92377.1"
                     /translation="MPTESKPAIILENVSVRYRLPSERIGTLKEYAIRLLQRRLKHRE
                     FWAIKDLDLNIQQGEVFGIVGDNGAGKSTLLKVISKVLRPTTGRVRIYGKIAPLLELG
                     AGFHPELSGRENVFLNGALLGYSHDEMNQVFNEIVEFSELAEFIDAPIRTYSSGMYAR
                     LGFAVATAHQPEILIVDEILSVGDEAFQRKCTDRITGFRENGATVLMVSHNLDLIAGM
                     CNRAAWLDHGKLIHMGNAEEVVAKYREGAH"
     gene            3647..4237
                     /gene="rfbC"
                     /locus_tag="PK791_03325"
     CDS             3647..4237
                     /gene="rfbC"
                     /locus_tag="PK791_03325"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOH92378.1"
                     /translation="MDFIPLEIPEVVLLVPKVFGDHRGFFLETFRDNQFREHVADFDF
                     VQDNHSGSSQGILRGLHYQIKQAQGKLVRAAVGEFFDVAVDLRQGSPTFGKWVGTILS
                     AENRHQLWIPPGFAHGLYVLSEWAEVLYKATDYYAPEWDRSLRWDDPSIGIKWPLING
                     HPPTLSQKDSDAPTLTNNAETYPIGWSAKPPCEEIK"
     gene            4234..>4900
                     /locus_tag="PK791_03330"
     CDS             4234..>4900
                     /locus_tag="PK791_03330"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOH92379.1"
                     /translation="MKKVLVTGGAGFIGSNFVHYLLKNHDDVFIVNLDALTYAGSLEN
                     LKDLPAPERHIFVQGQITDRELIDKLFAEHKIDTVIHFAAESHVDRSILNPGVFVDTN
                     VVGTFVLLEAARRAWLVDKIVPLEQCRFHHVSTDEVFGTLAPEDPPFQEDTPYAPRSP
                     YAASKAASDHFVRAYAETYGLPISISNCTNNYGPRQFPEKLIPLMILNAVSGKPLPIY
                     GDGM"
BASE COUNT         1249 a         1206 c         1097 g         1348 t
ORIGIN      
        1 tcatcaaatt cctgaagata gtcaatcgca gctgcaagac caatcgggcc aggcacgttt
       61 ggcgtgccag cttcgaattt gtaaggcaaa acgttaaagc taatcttttc aggtgtgaca
      121 ttactgatca tctcgccacc agagagaaaa ggtggcatgg catcaagcca tttttccttg
      181 ccataaagca cgccaacgcc catcgggcca tacattttat gacccgaaaa agcatagaaa
      241 tccgcatcca gcgcctgcac atcatgctcg atatgttgca cagcctgcgc cgcgtccact
      301 aacacgaccg cgccggcgtt atgggctaat tgaataattt cttttacagg gttgatggtt
      361 cccaacgagt tggaaacatg gttcaccgct accacacgtg ttttttcatt aagcaggctt
      421 ttgtaaacct caaggtctaa atcgccattt tcatcgaaag gaacgaccct gaatgttgct
      481 ttttttcgtt cgcagagcaa tttccagggc acatagttac tgtgatgttc cagttgcgtg
      541 acgataattt catcaccttc gcccacgaaa cgctcgccaa aactatatgc caccaaattg
      601 attgattccg tcgtgccacg cgtgaagata atttcgtgct tatgttcagc gttgatataa
      661 cccgcgacct tcgcgcgtgc tttttcgtaa gcctcggtag cttcgtaggc gatggcgtaa
      721 tttccacggt ggatattcgc attgtagcgc gtgtaataat cgcgaagggt gtcaatcacc
      781 acctgcggtt tttgggttgt cgcggcatta tcgagataga ccagtggtcg atcgttcacc
      841 tgtatatcaa ggataggaaa atcttgccgc gcagatagaa tgtctcttgc catattcttt
      901 cctttcagtc attcatttta ccaaaagaat acaggcttat tccagttagc gtagagttag
      961 aatttgcgtt tgataagtgg ggcaaaaaca ttccaattaa gcagtttggc gatgtgcata
     1021 acgctgactc tgcggttttt ttgaacaagc cgacgtttag cgataatcgt tttaagccct
     1081 ttgagtgaat cccattttgc tttagccatg actttagctt tgccgatgaa tggcgccaaa
     1141 agcagataag caagatttac caaaaaatgc gaaggcaaca aaagccaaaa caaccagcca
     1201 ggcatgtttt taatgaagac ccagggcaaa ttacgatggc catgataaac tgcaaaatca
     1261 ctgcggggcg cggtactggc tgaacccacg tgctttacaa cagcatttgg caggtataag
     1321 caaggatagc ccgcgagttg caaccgaaaa tcaaggtcaa tgtcttccat gtaggcgaag
     1381 aaatcttcgt caaacccacc gaccgcgtca aaggcttcgc gcgggtagac gctcgccgca
     1441 gcacaagcgc caaaaacctc tttgggttca tcactcgcgt ggattattgc ctggttatga
     1501 ttgtgccgcc aggcaagtcc actggcatgg taaacatgcc actcaccatc taacttgtcc
     1561 ggttccaaat acttgattaa tcgcgatgcg aagaaatgat ttggatttgc ttgcaaggct
     1621 tcattgatat ttgccagcca gtctggatct ggaaaagcat ccgcattaag caaaaccaaa
     1681 tacctgcctc ttacaaaacc agccaggtaa ttattccccg ctgcaaaacc caagttcttt
     1741 tccgaccgca agactttaac agacaaatta gcaaaatcat ccgcgaaatc cggcgccagt
     1801 ggaatctcgt tgccattatc caaaagaagt acttcgaaat ttttatacgt ttgggcgtcc
     1861 aaggcttcaa gacaggtctg caagtattgt tcagcccgcc aatgcagaat aagcacggag
     1921 aattgaatag gatttgcttt cgtggacatg gttaaattat agtgcataag atccgttttc
     1981 gtgaacactg ctggcatcat tcatgctttc ggtgctggct cattaaaatg actcttagat
     2041 ggcaagttat ggtaagattc tcagttgtga acacacgtac tgtctcagag aaaataaaac
     2101 cactttacga ctccagcaaa cgggtttcgc ctgccattgc tgagttaaaa gaaatcggta
     2161 actaccgcca tctcctggcg caactcattc gacgcgatat cttaacccgc tacaaacgct
     2221 ctttcctggg tgtagcttgg accatgctca accccctggg aacaatgctg gtcatgacca
     2281 ttgttttttc gaacatcttc agggcgagca tgcctggggt gcctttcccg gtttatattt
     2341 taagcggtct tgtcgtctgg aattttttct ctcagggcac tacagcttca atgagcggat
     2401 tagtgtgggg tggcaatttg cttcaacgca tattcgttcc cagaacgatt tttgccgttt
     2461 ctgccgttgg gactgctctg gtaaataccg tcatcgcgct cgtgccatta atcattgtca
     2521 tgcttatcac ccaagctaca ttcagctggg ctttattgtt tttaccaatt gccatcattc
     2581 ttgtagcatt tttcaccttg ggctttggct tgttaatttc ttcactcgca gcgtttttcc
     2641 cagatgttat ggagatgtac caaatcgtac tcacagcctg gatgtatcta acccctatta
     2701 tttatcctga aagcatcatc cccaaacatc tctttaatgt gtaccgactt aatcctatgt
     2761 actggctcgt taaaatcttt cgcgcgccta ttgttgacca gtctttgcca gccttgagcg
     2821 attttggcat cgcagctgct atttccatca ttatgcttgt cattggctgg cttgtattta
     2881 catcaaaatc ggatgaatat gcctacagaa tctaagcctg ccattatttt agagaatgtt
     2941 tccgtgcgct atcgtctccc gagtgagcgc atcggcacct taaaggagta tgcgattcgt
     3001 cttttacagc gtcgcttgaa acatcgtgaa ttttgggcga taaaagacct ggatctcaat
     3061 atccagcaag gggaagtatt tggcattgtt ggcgataatg gcgcaggcaa gagcacgctc
     3121 ttaaaagtaa tctcaaaggt tttgcgaccc accacaggtc gtgtgcgcat ttatggcaaa
     3181 attgcacccc ttttggaact tggcgcgggc ttccatcctg aactaagtgg ccgcgagaat
     3241 gtgtttttaa atggcgcgct tctcggttat agccacgatg agatgaacca ggtttttaat
     3301 gaaatcgttg aattctcgga attggctgaa tttatcgacg cgccaattcg cacttattct
     3361 tcgggtatgt acgcccggct cggcttcgcg gttgctaccg cgcatcaacc tgaaatctta
     3421 attgtggatg aaatcttgag cgtgggtgat gaggcttttc agcgcaaatg cactgatcgt
     3481 attactggtt ttcgtgagaa cggcgctaca gttttaatgg tctcacataa cttagactta
     3541 atcgcaggaa tgtgtaatcg cgccgcctgg cttgaccatg gaaaattaat tcacatgggc
     3601 aatgcagaag aagtcgttgc caaatacagg gaaggcgctc actagcatgg actttatccc
     3661 actcgaaatc cccgaagttg tcttactggt gcccaaagtt tttggtgacc atcgtggttt
     3721 ctttttagaa accttccgcg acaatcagtt tagagagcac gtcgctgatt ttgactttgt
     3781 tcaagataac cacagtggct caagccaggg cattttgcgc ggtctgcatt atcaaataaa
     3841 acaagcgcaa ggcaaactgg tgcgtgccgc ggttggcgaa ttttttgacg tcgcggttga
     3901 ccttcgccag gggtctccta cttttggtaa atgggttggc acaatcttaa gtgctgaaaa
     3961 ccgccatcaa ctttggattc cccccggatt tgcgcatggc ttgtatgtgc taagcgaatg
     4021 ggctgaagtg ctttacaaag ccactgatta ttacgcgcca gaatgggacc gcagcctgcg
     4081 ctgggatgac ccttccatcg gcatcaaatg gcccttaata aacggacatc ccccaacatt
     4141 gtcccaaaaa gattctgacg cacccacact aacaaacaat gcagaaacat atccgatcgg
     4201 atggtcggca aaaccacctt gtgaggagat caaatgaaaa aagtcttagt cactggcgga
     4261 gctggattta tcggttccaa cttcgttcat taccttttga agaatcatga tgacgttttt
     4321 atcgtcaatt tagacgcttt gacctacgct ggaagcctgg aaaaccttaa agaccttccc
     4381 gcgcctgaac gacacatttt tgttcaaggc caaatcacag accgcgaact gattgacaag
     4441 ctttttgctg aacataaaat tgacaccgtg attcatttcg ctgcggagtc gcatgttgac
     4501 cgctcgattc tcaatcccgg cgtttttgtg gacaccaatg ttgtgggcac atttgttttg
     4561 cttgaagccg cccgacgtgc ctggctggtg gataaaatcg tgccgcttga acaatgccgc
     4621 ttccaccacg tgagcaccga cgaagtcttt ggcacactcg cgccagaaga tccgcctttc
     4681 caggaagaca ccccctatgc tccgcgttcg ccctatgccg cttccaaagc cgccagcgac
     4741 catttcgtgc gcgcctatgc tgaaacctac ggtctaccca tcagcatcag taattgcacc
     4801 aacaactacg gaccccgtca attccctgaa aaactcattc cgctgatgat tctcaacgcg
     4861 gtaagtggca agcccttgcc tatatatggt gacggcatgc
//
LOCUS       DAOSFC010000090         4891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_15410_length_4891_cov_4.995658, whole genome shotgun sequence.
ACCESSION   DAOSFC010000090 DAOSFC010000000
VERSION     DAOSFC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15410_length_4891_cov_4.995658"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..680
                     /locus_tag="PK791_03335"
     CDS             171..680
                     /locus_tag="PK791_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92380.1"
                     /translation="MKKLSLFFIIPLLFVTACDPIKHPENSDKPSADIQVQHYDNDGE
                     VMTFNKKPPFGSDFLLRLDEEENEVYLPGNIAVKLTRIKDMRCPIGKECSQAGSVQVV
                     LEIDLVGEDPYEEILVLGGSLEDPSFLEFYGTTYLTLIAVEPVSTASETVKAEDQIVI
                     FSVTRYPIQ"
     gene            complement(684..1070)
                     /locus_tag="PK791_03340"
     CDS             complement(684..1070)
                     /locus_tag="PK791_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92381.1"
                     /translation="MEIELIKKYYNARGLAWPEQKDALLFYLSEVGELAEAYLAISPV
                     ALSDEERQLLADFAKLGMQADEIVSRVDGWVRNFDRQRKENIAHEVADCNMMLSVFME
                     SFAGRSPDDALREKMALKLGKSVDEL"
     gene            1106..2095
                     /locus_tag="PK791_03345"
     CDS             1106..2095
                     /locus_tag="PK791_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOH92382.1"
                     /translation="MATWKEKLSPYSKAVGDKKDVWQAKLKQAYYKINSGLKGVPGIV
                     RHAFTNLVRARVPEASAALAYYGLFSIFPALIAIVTIGSLFVDADVARSTLFQAASGI
                     FPVPETIAFVDAQLSAVLRVRGALTAIAAISLIWSASNVIDKVVLNVNRAFPNSSPSG
                     FFRNRGLALIVIVIILALFLASVMLAPLIKVLPAFKFAIKGVPFLETRLWKWISLVVP
                     LFFKFVMFYITYTWLPNSHSISRKARAYGALVAAILWEGVNKALTWAISRGFANYELV
                     YGSLASVMVLLFWIYWVGYILFFGAHLTNAINYHYKNNMARKKFDVVMEDSAL"
     gene            2092..2961
                     /locus_tag="PK791_03350"
     CDS             2092..2961
                     /locus_tag="PK791_03350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH92383.1"
                     /translation="MKKLSILIPTLNVCQMLKACLESIEQYTPAGTYEIIIADNASSD
                     GTLEMLQRDFPDLKVLTSDKNDGFSKPVNRLLKAAKGEYLVLLNPDTLLIEDVFSPQL
                     DWLAENEQVGICIPKVLNSDGSFQAQSKRGDAKPAAVFGYFLGLGKLFPQNRALNSYL
                     MSWLDEDEISEVDAVSGSCMIIRRECWEQVGDFDEAFFAYQEDSDYCIRARKAGWKVM
                     YVPISAIIHYGGQGGSKARPYKSIWEWHRSYFVLYKKHFAKDHFFLFNWFYYAMMGIK
                     LGLSLLANIFRRK"
     gene            complement(3047..4213)
                     /locus_tag="PK791_03355"
     CDS             complement(3047..4213)
                     /locus_tag="PK791_03355"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOH92384.1"
                     /translation="MNNFSYYNPTRILFGKGAIAKISRYIPKDAKVLMLYGGGSIKRT
                     GIYEQVKQALDGFTVLEFAGIEPNPDYATLMKAVQIVRDEKVDFLLAVGGGSVIDGTK
                     FIALAAAFDQGEAWDIIKTDAARELSQAVPFGTVLTIPGTGSEMNYNLVISRRETGEK
                     LSIKHEAIFPRFSALDPQVSYSLSAKQIRNGLVDAFMHVMEQYITYPIGAVVQDRLSE
                     SLMQSLIEVAPRAMRMDPPDYTARANFMWAAAMGLNTLVGRGVPQDWTTHMIGHELTA
                     LYGLAHAESLATVVPWLLWYKREQKGDKIVQLGMRVFGLISDDREALIEATIDALAAW
                     FNSLGMPTTLTAYGIDPDEAAEAVAKRFDDRGWALGENADIHGSDAAAILRLSR"
     gene            complement(4283..>4891)
                     /locus_tag="PK791_03360"
     CDS             complement(4283..>4891)
                     /locus_tag="PK791_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOH92385.1"
                     /translation="FWDSVNSLFDFLSGQATDGNTARITLWIAIGTVIIKIFMTIYTH
                     RVGKRTANATIIALAQDHRNDIFSALAVVIGVTFSLLGYRWVDPLAGALVAVMIFKTG
                     YDILRDSTDDLMDTLPGKVLTKQISDLTLKVPGVLGIDSIKAHRFGRYLVINIAIFVD
                     CCITVDAGDAIADEVEDILHEKIDFVQEVHVHFHSKSHRTRH"
BASE COUNT         1251 a         1104 c         1216 g         1320 t
ORIGIN      
        1 tggaacgcaa tattctcttt tgcgtggtga tgcgctcgga agtggcacgg ttgaaagaaa
       61 ttgtcaataa agctgaccca aacgcgttta tggttgtggg ccatgccaac gaagcactcg
      121 gccacggttt taaaccctta caagacttat aaatcaggaa cagacaaatt atgaaaaaac
      181 tcagcctttt ctttattatc cctttgcttt ttgtaacagc gtgtgaccct atcaaacacc
      241 cggaaaactc tgacaagcca tcagcggata ttcaagtcca gcattacgat aacgatggcg
      301 aagtgatgac tttcaataaa aagccaccgt ttggttcaga ctttctcttg cgactggacg
      361 aagaagaaaa tgaagtttat ctgccaggta atatagcagt gaagttgacg cgcattaaag
      421 atatgcgctg tccgattggc aaggaatgtt ctcaggcggg gagtgtgcaa gtggtattgg
      481 aaatcgacct ggtgggcgag gatccctatg aggaaatcct ggtcttgggc ggtagcttgg
      541 aagaccccag ttttttggag ttttacggga cgacttatct aaccttgatc gctgttgaac
      601 ccgtttcaac ggcatcggaa accgtcaagg cggaagacca aatagttatt ttttccgtca
      661 cgcgatatcc gattcagtga attttatagt tcgtccacag atttgccaag cttaagtgcc
      721 atcttttcgc gcagggcatc atcaggcgaa cgacccgcga atgactccat aaagacggaa
      781 agcatcatgt tgcaatccgc gacttcgtgg gcgatgtttt ctttgcgctg gcgatcaaaa
      841 ttccgcaccc aaccgtccac gcgcgaaaca atctcatcgg cttgcattcc cagcttagca
      901 aagtcagcca gcagttggcg ttcttcatcg cttagcgcga ccggactgat ggcgagatag
      961 gcctcggcaa gttcaccgac ttcggaaaga taaaaaagca aagcatcttt ttgttctggc
     1021 catgccagac cacgtgcgtt atagtatttt tttattaatt caatttccat agtacctctg
     1081 cgttatttta tcttataatc atagcatggc aacatggaaa gagaaattat cgccatatag
     1141 caaagctgtt ggggataaaa aggatgtctg gcaagcaaaa ctaaagcaag cctactataa
     1201 aatcaacagc ggcttaaagg gtgtgccagg cattgttcgt catgcattca ccaacctggt
     1261 cagggccaga gtgccggaag catctgcggc attggcgtat tacgggctct tttcgatttt
     1321 tcccgcatta attgctattg tgactatcgg gtcgcttttt gtggatgctg acgtggctcg
     1381 atcaacactt tttcaggcag cgagtggaat atttccagtg cccgaaacaa tcgcgtttgt
     1441 agatgcccaa cttagcgcgg tgcttagagt tcgtggcgcg ctgaccgcta ttgcggctat
     1501 ctcgctgatt tggtcagcca gcaatgttat cgataaagtt gtgctcaatg tgaaccgtgc
     1561 ttttcccaac agctcaccat cgggcttttt cagaaatcgt ggcttggcac tgattgttat
     1621 tgtgattatt ctggcgctgt ttttggcttc ggtcatgctc gcgccactga ttaaagtgct
     1681 cccggctttt aagtttgcta tcaaaggtgt gcctttcctg gaaaccaggc tatggaagtg
     1741 gatttccctc gtggtgccct tgtttttcaa gtttgtgatg ttctacatta catacacctg
     1801 gttgcccaac agtcatagca tttcgcgcaa ggcacgcgct tatggtgcct tggtagcagc
     1861 aatactctgg gaaggggtca ataaagcgct gacttgggcg atctccaggg gctttgccaa
     1921 ctacgaattg gtttacggct ccttggcgtc ggttatggta ttgttgtttt ggatttattg
     1981 ggttggttat attttgtttt ttggcgcgca tttaaccaac gccattaact atcattacaa
     2041 aaacaatatg gcacgaaaaa agtttgatgt cgtgatggag gactctgcgc tgtgaaaaag
     2101 ttgtcgattc tcattccaac cctaaacgta tgccaaatgc ttaaggcgtg tttggagtct
     2161 atcgaacaat atacgccagc tggaacatat gaaattatca tcgctgataa tgcttccagc
     2221 gatggcacat tggaaatgct gcaaagggac ttcccagatc taaaagtgct aaccagcgat
     2281 aagaatgatg gtttttcgaa accagtcaat cgccttttga aagccgcaaa gggtgagtat
     2341 cttgtccttt taaacccgga tacattactg attgaagacg tatttagccc acaattggat
     2401 tggcttgctg aaaacgaaca ggttggcatt tgtattccca aggtgcttaa cagtgatggt
     2461 agttttcagg cgcagtccaa gcgtggtgac gcaaaaccag cggcagtctt tggctatttc
     2521 ttaggtttgg gcaagttatt cccgcaaaat cgggctttga acagttatct gatgtcctgg
     2581 ctggatgaag acgaaatctc tgaagttgac gcggtttctg gctcgtgcat gattatcaga
     2641 cgcgaatgtt gggaacaagt gggtgatttt gatgaggctt tcttcgctta tcaggaagac
     2701 agcgactatt gcatccgtgc ccgaaaagct ggctggaagg tcatgtacgt acccataagt
     2761 gccatcattc actatggcgg acagggtggc tcgaaggcgc ggccttataa atcaatctgg
     2821 gagtggcacc gttcgtattt cgtgctttac aaaaagcatt ttgccaaaga ccatttcttt
     2881 ttgttcaact ggttttatta tgccatgatg ggcataaagc tgggattatc cttgctggcg
     2941 aatatcttta gaagaaaata aagcttgtta aaaattgttt aatgaaaagt ggctgttcat
     3001 atcggacagc cacttacttt tacctgttat ttaaactact ttatatttaa cggctaagtc
     3061 ttaaaatcgc agctgcatca gaaccatgaa tatcagcgtt ttcgcccagt gcccagccac
     3121 ggtcatcaaa gcgttttgcg acagcttcag cagcttcatc ggggtcaata ccgtaggcag
     3181 tcaaggtcgt gggcatgccc agcgaattga accacgccgc cagggcatca atggtggctt
     3241 caattaacgc ttcgcggtca tctgatatca aaccgaaaac gcgcattccc agttgcacaa
     3301 ttttgtcgcc tttctgttcg cgtttgtacc acaaaagcca gggcacgact gtagccagcg
     3361 attcagcgtg tgccaggcca taaagcgcag tcagttcatg tccaatcatg tgcgtcgtcc
     3421 agtcttgtgg tacgccacga cccaccaggg tgttgagtcc cattgctgct gcccacatga
     3481 agttcgcgcg ggcggtgtaa tcgggtgggt ccattcgcat ggcacgcgga gcaacttcga
     3541 tcagcgattg catcagcgat tcggacaagc ggtcttgtac gaccgcgcca atcggatagg
     3601 tgatgtactg ttccatgacg tgcataaaag catcaaccaa accattgcga atttgctttg
     3661 ctgaaagact gtaagaaacc tgtggatcca atgcggaaaa acgcgggaag atagcctcgt
     3721 gcttgatgga aagcttttcg ccggtttcgc gacgggaaat gaccaggttg tagttcatct
     3781 cactgccggt tccagggatg gtgagcacgg tgccaaaggg cacggcttgg ctgagttcac
     3841 gcgcggcatc ggttttgatg atgtcccagg cttccccttg gtcgaaagct gctgccaggg
     3901 cgatgaactt tgtgccatca atcacggaac ccccaccaac agccagcaga aagtcaactt
     3961 tttcatcacg aacaatttga acagctttca tcagtgtggc gtagtcgggg ttgggttcaa
     4021 taccagcaaa ttcaagcaca gtgaagccat ccagggcttg cttaacctgt tcgtaaatgc
     4081 cagttctttt gatggacccg ccgccataaa gcatgagcac tttggcatct ttggggatat
     4141 aacgggaaat cttcgcaatc gcgcctttgc caaagaggat acgggtcgga ttgtaatagc
     4201 taaagttgtt catttggtct ccttttttga tctcttaaat gataacgaag ttgctttttg
     4261 attcaaaatg tgtgagaaaa gtttagtgtc gcgtccggtg tgatttggag tgaaaatgca
     4321 catgcacttc ctggacaaaa tcgatcttct cgtgaaggat gtcttcaacc tcatcagcga
     4381 tggcatcgcc ggcatccaca gtaatacagc aatccacgaa gatggcgatg ttgatgacca
     4441 gataacgtcc aaagcgatgg gcttttatcg aatcgatgcc aagaacaccg ggcaccttga
     4501 gcgtcaagtc gctgatttgt ttggtgagaa ctttgccagg cagggtatcc atcaggtcat
     4561 cggttgagtc gcgcaaaata tcataacccg tcttgaaaat catcaccgcg accaaagcgc
     4621 cagccagggg gtcaacccag cggtagccaa gcagactaaa ggtcacgcca ataacaacgg
     4681 ccagagcact aaaaatatcg ttgcggtggt cttgagccag ggcgatgatg gtggcgttgg
     4741 cggtgcgttt gccaacacga tgggtgtaga tggtcatgaa aattttgatg atgaccgtgc
     4801 caatggcaat ccaaagtgtg attcgcgcgg tgtttccatc agtggcttga ccacttaaaa
     4861 agtcaaaaag cgaattgacc gaatcccaaa a
//
LOCUS       DAOSFC010000091         4837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_15642_length_4837_cov_3.916562, whole genome shotgun sequence.
ACCESSION   DAOSFC010000091 DAOSFC010000000
VERSION     DAOSFC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15642_length_4837_cov_3.916562"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2237)
                     /locus_tag="PK791_03365"
     CDS             complement(<1..2237)
                     /locus_tag="PK791_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOH92386.1"
                     /translation="MFIPTTPEELKKLGWDSLDVILVSGDTYIDSPYSGVALIGHQLI
                     RAGYKTGIIAQPNTTNYIDIARLGVPRLFWGVTAGMVDSMVANTTALGKPRRMDDNTP
                     GGLNNRRPNRATLVYTNLIRRWFKNTAPIVLGGIEASLRRLSHYDFWSNSLRRSVLLD
                     SKADYLLYGMADKSILELAEILKMRGDARGIRGLCYLSPTVPKDYIELPSFEACELSK
                     QKFIDMFNTFYANNDPITAKGLAQKSGSRYVIQNPPAMPLTTEEMDYVYNAPFENAVH
                     PYYRAMGEVRAMDTIRNSIVTHRGCYGECNFCAIASHEGRHISWRSKESILKEAKQLS
                     SRKDFRGIIHDLSGPTANMYGFDCEIKRQKGACKNRSCIYPEVCPSLKLTHLPHLELL
                     RAVRDLPGVRKVFVGSGIRYDMVMADKLHGRAYVRELAGHHVSGQLKLAPEHSEPNVL
                     ACMRKPHTQALLDFKKAFELESEAHGLEQYLTYYLIAAHPGCTKEDMIQLQEFAASEL
                     KVMPEQVQIFTPTPSTYASLMYYTETDPFTGKKIFVEKTDTGKMQQKALITGWRGKKS
                     RQVDEREARGTDRGDKRGDKRGDKRDRPKKRDGDEPRRETRIVRDDDGRFFMNRKPEG
                     WVDKKSKDYKKKPKTSRAQSESRPYDPERKPRPTDGDGEKSPRPRKPFDPKRRPPHAD
                     DDKAQGARKPYDPKRKPPRADGDKPQGARKPYDPKRKPPRADGDKPQGTRKPYSQKRD
                     PKDKR"
     gene            complement(2420..3445)
                     /locus_tag="PK791_03370"
     CDS             complement(2420..3445)
                     /locus_tag="PK791_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92387.1"
                     /translation="MKKKTAFLIATISLCLVLSLTSCSFLAKTLRAGADRIEQESAKT
                     EELGVEDQVASLVADISTQTKAAQLETPVPMEPAVNTPVPSPTIESIVPPTVTPQPGQ
                     TSTDTPSNKPDKEIRLMGLSFTLPGDLDYTVTIEELDEWPDPNTLEPGNIGPARIIFH
                     LNPSYDHQHFHDGPRIYVFPGYSYAKYYDVAMSFFQLNMLMDTEDFSDYDVDEALPFP
                     PLFNAMQMFHSNEKVIHFQNGSGIRYLTAYHQIYAPIVTQDLFYAFMGFTEDHNYIVV
                     AIIPIYESPLPWSTPLPPDGDPFYSNYTQHLLDTANALNAASPADFNPNLDLLDKLFE
                     SLRLSMQ"
     gene            3697..4725
                     /gene="mvaD"
                     /locus_tag="PK791_03375"
     CDS             3697..4725
                     /gene="mvaD"
                     /locus_tag="PK791_03375"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOH92388.1"
                     /translation="MKDKNHSQKPTHSATAIAHPNIAFIKYWGNRDDALRLPLNSSLS
                     MNLDGLETRTTISFDESLNADSLTLNGILQEGAGLARVSAFLDSARELAVQRGILKPE
                     LQKRYFAQVVSENSFPAGAGIASSASAFAALAMAVDGALGLNLPEAQLSRLARLGSGS
                     ASRSIPTGFVEWHAGQDDESSYAESFAPPSHWDLVDMVVIVDRGHKQVGSSQGHPLAR
                     TSPLNDLRVAGVDERLRVCKEAILARDFEAFAWATELDSEWMHAVMRTSTPSLRYWTA
                     RTETVLWQVRYWRQKGHALCTTVDAGPNVHVIGTQDELDFALPALKALPNVIDVIVAK
                     PGAGASLV"
BASE COUNT         1093 a         1060 c         1354 g         1330 t
ORIGIN      
        1 tgacgcttgt cttttggatc gcgtttttgc gaataaggtt tgcgtgtgcc ctggggtttg
       61 tcgccatcag cacgtggtgg tttgcgcttg gggtcgtaag gcttgcgcgc gccctggggc
      121 ttgtcaccat cagcacgtgg tggtttgcgc ttggggtcgt aaggtttgcg cgcgccttgg
      181 gctttatcat catcagcatg gggtggtctg cgcttggggt cgaaaggctt gcgcgggcgt
      241 ggggactttt caccatcacc atcggtaggt cttggtttgc gttctgggtc gtagggtctg
      301 gattcagact gtgcccggct tgttttcggc ttctttttgt agtctttgga ttttttatct
      361 acccagcctt cgggcttgcg gttcataaag aaacgcccat cgtcatcgcg cacgatgcgg
      421 gtttcacgac gtggctcatc gccatcacgc tttttggggc ggtcgcgctt atcaccgcgc
      481 ttatcacctc gcttatctcc gcgatcggtg ccacgggctt cgcgttcatc aacctgacgg
      541 gactttttgc cacgccaacc ggtaatgagg gccttctgct gcatcttgcc ggtatcggtt
      601 ttttccacaa aaatcttctt cccggtgaag gggtcagtct cggtgtagta catcaaactg
      661 gcgtaagtgg atggtgtcgg ggtgaagatt tgcacctgct cgggcatcac ctttagttcg
      721 ctggcagcaa actcttgcag ttgaatcatg tcttcttttg tgcaacccgg gtgggcagca
      781 atgagataat aggtcagata ctgttcaagg ccatgcgctt cgctttcgag ttcaaaggct
      841 tttttgaagt ccagcagggc ttgggtgtgc ggtttgcgca tacaggcaag cacgtttggc
      901 tcggagtgct cgggcgcgag tttaagttgg ccagatacat gatgcccagc cagttcacgg
      961 acataggcgc ggccatgtag tttatctgcc atgaccatgt catagcgaat gccagacccc
     1021 acaaagacct tgcgcacgcc tggcaaatcg cggacggcgc gcaggagttc aaggtgtggc
     1081 aggtgggtca gctttagact ggggcatact tccgggtaaa tgcagctgcg atttttacaa
     1141 gcgccttttt ggcgtttaat ttcgcaatcg aagccgtaca tgtttgctgt tggaccggaa
     1201 agatcatgga tgataccgcg gaaatctttg cggctgctga gttgtttggc ttcctttaag
     1261 atagattctt tggaacgcca gctgatgtga cgtccttcgt gtgaagcaat cgcgcaaaaa
     1321 ttgcactcgc cataacagcc acgatgggtg acgattgagt tgcgaatggt gtccatcgcg
     1381 cgcacttcgc ccatggctct gtagtagggg tgcaccgcgt tttcaaatgg cgcgttgtag
     1441 acataatcca tttcttcggt tgtcaaaggc atggcgggtg ggttttgaat gacgtaacgg
     1501 ctacccgatt tttgggcgag gcctttggca gtgatggggt cgttgttggc gtaaaaggta
     1561 ttgaacatgt caataaactt ttgtttgctc aactcgcagg cttcgaaact gggcaattcg
     1621 atataatctt ttggcactgt tgggcttaga tagcacaaac cgcgaatgcc acgcgcgtca
     1681 ccgcgcattt tgagaatttc ggcaagttcc aggatgcttt tatctgccat accgtaaagc
     1741 aaatagtccg ctttggagtc caacagaaca gaacggcgca aggagtttga ccaaaagtca
     1801 taatgactga ggcggcgcag tgaggcttca atcccaccca aaacgatagg cgcggtgttt
     1861 ttgaaccagc gtcgaattaa attcgtgtag acaagtgtgg cacgattggg ccttcggttg
     1921 tttaggccac caggggtgtt atcgtccatg cggcgcggtt tgcccagggc agtggtgttg
     1981 gcgaccatcg agtccaccat acccgcggta accccccaga acagacgggg aacgccaaga
     2041 cgggcgatat caatgtagtt tgtggtgttg ggctgggcga tgatgcccgt tttgtagcca
     2101 gcgcgaataa gctggtggcc gatgagtgcc acgccactgt aggggctatc gatgtaggta
     2161 tcgccactga ctaaaatgac gtcaaggcta tcccatccga gtttctttaa ttcttcgggg
     2221 gtggttggta taaacatgcc ataatgatac cttaatcgtc cttgctgaga aaggttggat
     2281 tttgattttt atagtgttaa ttttacagaa tgataccctg tgttttacag gtttaatgta
     2341 acgataaatg taaaacgcta tagaaaaacc ctgcccagag atttgagcag ggttgatttc
     2401 agaactgaaa agcaaaattt tattgcatgc ttagtcttaa gctttcaaag agtttatcca
     2461 gcaagtccag gtttgggtta aaatcagcag ggctggcagc attcaaggca ttggctgtgt
     2521 ccagtaaatg ttgggtgtaa ttgctataga aaggatcgcc atccggaggg agcggtgtac
     2581 tccagggcag tggcgattcg taaatcggta ttatcgcgac cacaatgtaa ttatggtctt
     2641 cagtaaaacc catgaaagcg tagaacaagt cctgggtaac gataggcgcg taaatttggt
     2701 gatatgccgt taagtagcgg atgccggagc cattttggaa gtgaatgact ttctcgttgc
     2761 tgtggaacat ctgcatggca ttaaaaagcg gggggaaggg cagtgcttcg tcgacatcat
     2821 aatccgaaaa atcttccgtg tccatcagca tatttagctg gaaaaaggac atagcgacat
     2881 cataatactt cgcatagcta tagccaggga aaacatagat tctgggtcca tcgtgaaagt
     2941 gttgatggtc ataagatgga ttgagatgga aaataatgcg ggctgggcca atattgccgg
     3001 gttccagggt gttggggtcg ggccactcat caagctcttc aatggtcacc gtatagtcaa
     3061 ggtcacctgg aagcgtgaaa cttaatccca tcaggcggat ttccttgtcg ggtttgtttg
     3121 atggcgtgtc agttgatgtt tggcctggct ggggtgtgac ggtgggcgga acgatggatt
     3181 caatcgtagg gcttggcaca ggtgtattga cagctggttc catcggcaca ggcgtttcca
     3241 gctgagccgc cttggtttga gttgagatat ccgcgaccag gctggcaacc tggtcttcca
     3301 cgccgagttc ttcggtttta gcgctttcct gttcaattct atcggcaccc gctcttaaag
     3361 tcttggctaa aaaactacag gatgtcaacg aaaggacaag gcaaagactt attgtggcga
     3421 taagaaaggc agtcttcttt ttcatttctc ctccaaagct tgtagcttgt aatgaattaa
     3481 ggttctgtaa ctcaaaatat gggacaatct attattattt taaagtttac catattttat
     3541 taaacacaac tttgttttca aagatgtctc ttcatatttt tttcaccgaa attggtaaaa
     3601 aagcttatgc gattaactaa aacgaattgt ttttatcggt acattgttct ttaattaaga
     3661 cacaactacg accctgtttg caggtataat ttggttatga aagataaaaa ccacagccaa
     3721 aaaccaactc attccgctac tgccatcgcg catccgaata tcgcttttat aaagtattgg
     3781 ggcaatcgtg atgacgcctt gcgtttgcca ctcaacagtt cgctttcgat gaacctggac
     3841 ggacttgaaa cgcgcacgac gataagtttc gatgaaagtt tgaacgcaga cagcctgact
     3901 ttgaatggca tcttgcagga aggcgcgggt ttggcgcggg tttcagcgtt tttggactcc
     3961 gcgcgtgaat tagctgttca gcggggcata ttaaagcctg aattgcaaaa acgttacttt
     4021 gcccaggttg tcagcgaaaa cagttttcca gccggggcag gtattgcttc gtcggcatcg
     4081 gcgttcgcgg ctttggcgat ggcggtggat ggcgccttgg gcctgaactt gcccgaagcc
     4141 caactttcgc gcctggcgcg tttggggtct ggctcagctt cacgttcaat accgactggt
     4201 tttgtcgaat ggcacgccgg gcaggacgat gaaagcagct acgcggagag ttttgcccca
     4261 cccagtcact gggatttggt ggatatggtg gtcatcgttg accgtggtca taagcaggtg
     4321 ggctcttcgc aggggcatcc gctggcgcgt acttcgccac taaacgactt gcgcgtagct
     4381 ggtgtggatg agcgtttgcg ggtttgcaaa gaagccattt tggcacgcga ttttgaagct
     4441 tttgcctggg cgacggaatt ggattcggag tggatgcacg cggtgatgcg caccagcacg
     4501 ccttcattgc gctattggac cgcgcggaca gagacagttt tgtggcaggt gcgttactgg
     4561 cgacagaaag gccacgcgct gtgtaccacg gtggatgccg gtccgaatgt gcatgttatc
     4621 ggtacgcagg atgaacttga ttttgccttg ccggctctta aggccttgcc gaatgtgata
     4681 gatgtgattg tcgccaaacc gggtgccggg gcgagtttgg tttaatggtt gtgtgtaatg
     4741 gataaaggag agaaataaga tgacaattcc agattttcag acaattatgt tgccactact
     4801 aaaactatca tctgatggtg aagaacatta cattcat
//
LOCUS       DAOSFC010000092         4795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_15836_length_4795_cov_5.550000, whole genome shotgun sequence.
ACCESSION   DAOSFC010000092 DAOSFC010000000
VERSION     DAOSFC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15836_length_4795_cov_5.550000"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..938
                     /locus_tag="PK791_03380"
     CDS             183..938
                     /locus_tag="PK791_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOH92389.1"
                     /translation="MNNPHKYPKIIAHRGASAHAPENTLPAFQLALEQSADGIELDVM
                     LSKDNRLIVIHDSTLERTTNGKGNVLDHSYAELKELDAGYHFGDAFKNTPLPLLDEVY
                     EQFGGKFKINVELKNYHRPYDQLPDIALALTKKHNLLDTVIFSSFNARNLIKLRKAEP
                     KVQTGLLCLPGFAGALYRGGFGRRYRYDALHPHHSDVSQNLVQSLHKRGKQINVWTVN
                     KTDDLLKMAGFGVDSIITDDPEHARKVLDNLNI"
     gene            1024..3786
                     /locus_tag="PK791_03385"
     CDS             1024..3786
                     /locus_tag="PK791_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOH92390.1"
                     /translation="MSAEEQIILEMLQRMSPLEKAGQLMLVTFDGTDVSENSEIIELI
                     QDYHIGGVVLSTKNENFTDVDTAQATQALITALQESAYQKSLGLPATGHLKDGEPVYI
                     PLYVAHLHAQADNHSERLLPALNNLPSEMTLGASWSPELSNAIGQIYGAELSSLGFNL
                     FLGPNLDLIDTSDTSKGIFTGTSSFGANPYWVGKIANAFVGGIHTGSNNRMAVVATHF
                     PGLGGADRPINQEVSTIQRSLDQLVQNELAPFLALTGASHDATTVDGVMTSHIRFQGL
                     QGNVLSTTKPVSFDPIALQQLFSVPPLNTWQANGGLTVSDSLGNPAVRSFFDPTGLSF
                     DAPAIARTAFLAGNDILYLDNFATSIYESQIETIKRTISFFAQKYQEDTVFAQRVDES
                     VGKILKNKLGLYDSFSLKNVISDTESEVQDQTLNQLNLEIARESLTLLAPKEDYLNTI
                     FNEGPRTTDYITVFSDTRSVLPCETCSRINTLGVDDFKNTLLGLYGSQGTNQIVDYRI
                     NSYTFAQLSDFLNDVENEKSSYLEEHLKRARWVIFNVQNANTEIPASLALKHLLAEHA
                     NLLRDKMVIVFAYRAPFYLDSTEVSKLTAYYGLFTPSKASLELAARVLMHEARAKSAL
                     PYSLPAVAYDLQYQLAPDPKQIINLDLVTDTVPVNPDLATPLATDTIIGPEVTQTPVP
                     LFRLGEAVRIQAGPILDRNGNIVPDGTLTKFTIKMVSDALILALPEATTVNGYATIEY
                     RVEREGIFAVTASTGEASTSATLILTTQGGLAEIIMPTSTPMPEPSRTPEPTPTPQPT
                     PAPVSDLEEGFVSHPTGYPSVNDWLLTIMLLITGFGISYAIGYFWWKGRQWAIRSGLC
                     TVLGGLVAYLTLSLGVPWIIEVIRKSGTWFVIQTSIVGMLIGWIASLIWWLGYQARKK
                     KLDIPN"
     gene            complement(3769..4791)
                     /locus_tag="PK791_03390"
     CDS             complement(3769..4791)
                     /locus_tag="PK791_03390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOH92391.1"
                     /translation="MFLTLDKKTWMKQDFCSGSILHDRYKVERVIGQGGYGSILLAED
                     LRLSGRFCAVKQVSYDPNYTPTMLEDAREQFMREATVLARLDHPNLPKVSDFFSIDDN
                     DFLVMDYIPGDDLRAVIAKAEANKQFLNEADVIDWAMQIGDALSYLHSQNPAILHRDI
                     KPSNIKVTPAGVVKLVDFGLVKLLAPGEVTITIMQGQGTALYSPLEQYGGDSNMMDGR
                     ADIYAFGSTLYHLLTNTPPVNVRDRFLDPSNLVPPREINPAISPRVEDAILWAMELHP
                     NERPRNVKTFLKALTGESTSSLRHLAGKVNFREYQGLDRTEMILLYIAGGLTFFSLLL
                     TLIRNI"
BASE COUNT         1287 a         1309 c         1039 g         1160 t
ORIGIN      
        1 ctctaaccta gcggttaaca gccgcccgct ctgccgattg agctaccagg gaaggtagaa
       61 cgctattata acgaagcccc cataaaaaag tcaagcactt tttcctggtt cattctgaat
      121 cctaataaaa cgcaacacaa gttttgcttc tgaacccgtt acttgatgct ataatttgag
      181 acatgaataa tccacacaag tatccaaaaa tcattgccca ccgtggcgcg tccgcccacg
      241 cgcctgaaaa caccctacca gcttttcaac tggcgctgga acaaagcgcg gatggcatcg
      301 aactggatgt gatgctcagc aaagataacc gtctgattgt tattcacgac tccaccctgg
      361 aacgcaccac caacggcaaa ggcaatgtgc ttgaccacag ttacgccgaa ctcaaggaac
      421 ttgacgcggg ttatcacttt ggcgatgcct tcaaaaacac gccccttccg ctgcttgatg
      481 aagtttacga acaatttggc gggaagttta aaatcaatgt ggaacttaag aattatcacc
      541 ggccttacga ccaactccct gatatcgcgc tggcactcac caaaaagcac aatcttttgg
      601 ataccgtcat tttttcatcg ttcaatgcgc gcaatctgat aaaactaaga aaagccgaac
      661 caaaagtaca aacgggtttg ctttgcctgc caggtttcgc cggtgcgctt taccgtggcg
      721 gtttcggccg tcgctaccgc tacgacgcgc ttcacccgca ccatagcgac gttagtcaaa
      781 atctggtgca aagcctgcac aaacgtggca aacaaatcaa tgtgtggact gttaacaaaa
      841 ctgacgacct acttaaaatg gcaggctttg gcgtggattc gatcatcacc gatgacccgg
      901 aacatgcccg caaggtgcta gacaacttaa atatctgata tgcgaaaatt tctgctcttg
      961 ttcttaacgc tctgcctctt ggctcccctt gcgtccgcgc acgcgcaagg agaaacgcca
     1021 ggcttgtcag cagaagagca gataatcctc gagatgcttc agcgcatgag cccgcttgaa
     1081 aaagccggac aactgatgct ggtaactttc gatggcacag acgtttcaga aaacagcgaa
     1141 atcatcgaac ttatccagga ttaccatatc ggtggggttg tgctcagcac caaaaacgaa
     1201 aatttcactg atgtggatac cgcgcaggcg acacaagctt taatcactgc cttgcaggaa
     1261 agcgcgtatc aaaaaagcct tggtttgcct gcaacgggtc atctaaaaga cggagagccc
     1321 gtttatatcc ccttatacgt cgctcatctg cacgcgcagg cggacaatca cagtgaacgc
     1381 ttgttgcctg cattaaacaa tctgccaagc gaaatgaccc tgggcgcaag ctggtcgcct
     1441 gaattaagca acgccatcgg gcaaatttac ggcgcagaac tgtcgagttt gggcttcaat
     1501 ctctttttag gacctaacct ggacctcatc gacaccagcg acacaagcaa aggcattttt
     1561 accggtacca gcagttttgg cgccaaccct tactgggtcg gaaaaatagc caacgctttt
     1621 gtgggcggga ttcatacagg cagcaacaat cggatggcgg ttgtggcaac gcattttccg
     1681 ggactggggg gtgccgaccg gccgatcaac caggaagttt ccacgattca acgcagttta
     1741 gaccaacttg tccaaaatga actggcgcct tttctcgcgc tgaccggtgc cagtcacgat
     1801 gctaccacag ttgatggcgt catgacttca catatccgat ttcaaggtct gcaagggaat
     1861 gtgctcagca ccacaaaacc ggttagtttt gaccccatcg ccctgcaaca gcttttttct
     1921 gtccctcccc tcaatacctg gcaagctaat ggcggtttga cggtcagcga cagcctgggc
     1981 aatcccgcgg tacgaagctt ttttgaccca actggtcttt catttgacgc acccgcaatc
     2041 gcccgcactg cttttttggc ggggaacgat atcctctatc ttgacaactt cgctacttct
     2101 atctatgaat cccagattga aaccatcaaa cgcaccatca gcttttttgc ccaaaaatat
     2161 caggaagaca ctgtttttgc ccaacgggtt gatgaatctg tgggcaaaat tcttaaaaac
     2221 aaacttggtc tttacgattc gtttagcttg aaaaacgtca tatctgacac cgaaagtgaa
     2281 gtgcaagacc aaaccctaaa ccaacttaac ctggaaatcg cccgcgaaag tctcaccctg
     2341 ctcgccccca aagaagacta cctaaacacc atctttaacg aaggcccccg aacaacggac
     2401 tacatcaccg tttttagcga tacacgcagt gttttgccct gtgaaacatg ctcaagaatt
     2461 aacaccttag gcgtagatga ctttaaaaac accttactcg gcttatatgg ctcccaggga
     2521 acaaaccaaa ttgtcgatta ccgtattaat tcttacactt ttgcccaact ctccgatttt
     2581 ctcaatgacg ttgaaaacga aaaatcatct tatctggaag aacacctcaa acgagcacga
     2641 tgggttattt ttaacgttca aaacgccaac acggaaatcc ccgccagttt agccttaaaa
     2701 cacctattag ctgaacacgc taatctcctt cgcgacaaaa tggtcatcgt gttcgcttac
     2761 cgggcaccct tttacctgga ttccaccgaa gtttcaaaac tcaccgccta ttatggtctt
     2821 tttaccccat caaaggcatc tttggaactc gccgcacgcg ttctcatgca cgaagcccgc
     2881 gccaaaagcg ctttacctta ttccttaccc gctgtagcat atgacctgca atatcagctt
     2941 gccccagacc caaagcaaat catcaatctg gacttagtca ctgatacagt accagtcaat
     3001 ccagaccttg ccacacccct tgccacagat acaattatag gccccgaagt tacacaaacg
     3061 cccgtgccac ttttcaggct tggcgaagcg gtgcgtattc aagccggacc tatccttgac
     3121 cgcaacggta acatcgttcc cgatggcacc ttaaccaagt ttacaatcaa gatggtcagc
     3181 gacgcgctta ttctcgcgtt acccgaagcg acaacggtta atggttatgc cactattgaa
     3241 taccgtgttg agcgggaagg catttttgcg gtaaccgcca gcactggcga agccagcaca
     3301 tctgccaccc ttattctcac cacccagggc ggtttggcgg aaatcatcat gcccacttcc
     3361 acaccgatgc ctgaaccaag tcgcacgccc gaaccaacgc ctacgcccca gcccacacca
     3421 gcccctgtgt ctgatcttga agagggattt gtcagtcatc ccacaggcta tccaagcgta
     3481 aacgactggt tgctaaccat catgttgctt attactggtt ttggaatcag ctatgccatc
     3541 ggctacttct ggtggaaagg cagacagtgg gcaattcgtt ccggactttg cactgtcttg
     3601 ggtggcttgg tggcgtatct cacgctcagc ttgggtgttc catggataat tgaagtcatt
     3661 cgcaaaagtg gcacatggtt tgtgattcaa accagcattg tgggcatgct gattggctgg
     3721 attgcttcac tcatctggtg gttaggctat caagcacgta agaagaaact agatattccg
     3781 aattaaagtc agcaacaaac taaaaaaggt cagtcccccg gcgatataaa gcaggatcat
     3841 ttctgttctg tccaggcctt gatactctct gaaattcacc ttgcccgcca aatgacgcaa
     3901 actgctggta gattcaccgg tcaaagcttt taagaaagtt ttcacgttcc gcggacgttc
     3961 gtttgggtgc agttccatag cccacaggat agcatcttca acgcgcggag aaatggcagg
     4021 gttaatctca cgtgggggta ctaagttgct ggggtctaaa aagcggtcgc gtacgttcac
     4081 ggggggtgtg ttggtaagca aatgatagag cgtgctgcca aaagcgtaaa tatcagcgcg
     4141 accgtccatc atgttcgaat caccaccgta ttgttccaag ggcgaataaa gcgcggtgcc
     4201 ttgcccctgc ataatcgtaa tcgtcacctc gccaggcgcc aaaagtttta ccaagccaaa
     4261 atcaaccagt tttaccaccc cagctggtgt cacctttatg ttagatggtt tgatatcccg
     4321 atgcagaata gcagggtttt gactgtgcaa atagctcaaa gcatcgccaa tttgcatcgc
     4381 ccagtcaatc acatcggctt cgttcaggaa ttgcttgttc gcttctgctt tggcaatcac
     4441 agcgcgcagg tcatcaccgg ggatataatc catcaccaaa aaatcattgt cgtctatcga
     4501 gaaaaagtca gatactttag gcagattcgg gtgatccaaa cgcgccaaaa ccgtcgcttc
     4561 acgcataaac tgctcacgcg cgtcttcaag cattgtggga gtgtagttag ggtcgtaact
     4621 cacctgcttt accgcgcaga aacgcccgct aagccgcaaa tcttctgcca gcaaaatgga
     4681 gccatagccc ccctgaccaa taacccgctc caccttataa cgatcgtgca aaatgctgcc
     4741 gctacaaaaa tcttgtttca tccaggtctt cttgtctaaa gttaaaaaca ttttt
//
LOCUS       DAOSFC010000093         4664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_16452_length_4664_cov_4.913430, whole genome shotgun sequence.
ACCESSION   DAOSFC010000093 DAOSFC010000000
VERSION     DAOSFC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16452_length_4664_cov_4.913430"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PK791_03395"
     CDS             <1..969
                     /locus_tag="PK791_03395"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOH92392.1"
                     /translation="HAQGAICYTISPFHTVEYFAKIAVDLESMGADSICIKDMAGILT
                     PKIAADLVVAIKERTKLPVNMHTHATSGTGAMTYLDAVQIGADMIDTAISPLSDGTSQ
                     PPTETMHIVLSEMNFDTGLDGDKLVKIASYFKGVRKTFQSAKIFDDKVYQVNPLILQY
                     QVPGGMLSNLIAQLKEVGAENRLDDVLAEVERVRSEVGYPPLVTPMSQIVGAQAVVNI
                     LSGSRNTIIPNEMQDYVRGYYGRPAAPINPEFAAKILQGEKPITQRPADLLEPRLPKA
                     RKEIEHLHPSDESVISYAMFPEITKAYLERHQVTSEYGWANAGALG"
     gene            1145..1654
                     /locus_tag="PK791_03400"
     CDS             1145..1654
                     /locus_tag="PK791_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOH92393.1"
                     /translation="MKSNHPLDLEQSFDQLYRENLRPLFQYLYSRVQDIHLAEDLCSQ
                     TFISAFESLDRLKSPDKFKPWLFTIARNKANDHFRKLKRQPMLELDEIIATHPLQEEA
                     GNNNKQDQRILLEQLFARLKPAEQELLRLKLVAGLTFKDIAILLKSNENNVKKSYYRL
                     LERLQAQVE"
     gene            1659..2762
                     /locus_tag="PK791_03405"
     CDS             1659..2762
                     /locus_tag="PK791_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HOH92394.1"
                     /translation="MKTQQFEEKIQSSFALPEPSPEFEERLARTLRAKVHAQKITPRR
                     QVQVPRAWAYAFATLAALVIVVFALGPQKVLAQIQSWLGFLPGIGLVSDDASSLRVLA
                     EPASQTLDGITVEVSEAVASSDKTYVNYQVFGLTNQHFPAQESDPGCHFEPNLKLPNG
                     TNLAFANGYYEAVPKEINQVVLHLACIPGTDSAKTPSNWQIPFTLKTADDLVTMPVLL
                     PEPQVYRQSDQTFGEAIDDSSALHIHTVVETPKGYIMTGWVETDLNIALGPMNPKLID
                     SNGKEVQTRLSQQASDLMQNIPAEHQFYAWGFEFDSEGLSFPLTVSFSVLPKADQNMF
                     EPIHFMVDLGANPQMTRYGNSSRHLALVQLKLP"
     gene            2768..3898
                     /locus_tag="PK791_03410"
     CDS             2768..3898
                     /locus_tag="PK791_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92395.1"
                     /translation="MQYLSQNRLKLFFTATNGLIDLDIQFPDLQVSGGGGGGHSDASD
                     SSMSFERSFVFETTPKGIVEILFTDPVFAQDAVDFSSVWQPATPHAPYPEMATHTCPS
                     LAIDSSQSNFPVFDQAYIALYRQISGNPVKYELIYRNSQATEGLVLSDNAVWGSISPD
                     STQVLFPKGLEGSTLQNLSDNQKQILAGIIANDSKWSPDGNYIAYLRGDGAVFNMPAF
                     AKVDGTEANTISELGYAMIAGWSPDSRTIYFTLPWFNSSSWKVMAYDIESDETKELFT
                     IQNGTIKFLDPQISPDGKWLSYRGVDNSSLYLVSLDGETERLILDDAHVVKHIWLDDT
                     HIALSISYFSGTYENYVLNLNDCSFMRLDDLDGGLVDMQLKN"
     gene            complement(3982..>4664)
                     /locus_tag="PK791_03415"
     CDS             complement(3982..>4664)
                     /locus_tag="PK791_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOH92396.1"
                     /translation="ADTGKLSMMRSKEGEADYRYFREPDLQPVILSNEEIEAIKASLP
                     ELPYARRRRFVEEYQLSAYDAEVLTSERGLSDYFEEAVALYTKEPKTISNWMMNDMIR
                     LMNDTGKSARDLVLKPAALVEIVKLIDDGKINAATGKSLLVKVEQTGKSPATIVEEEG
                     LGLIADADSLRKQIETILAGAPNEVAAYRGGKETLMGWFVGQVMRATRGKADPKLTRE
                     ILLELLKD"
BASE COUNT         1236 a         1232 c         1036 g         1160 t
ORIGIN      
        1 cacgcccagg gcgccatctg ctacaccatc agccccttcc acaccgtgga atacttcgcg
       61 aaaatcgctg ttgaccttga gtcaatgggc gcggattcga tctgcatcaa agacatggcc
      121 ggcattctca ccccgaaaat cgccgctgac ctcgtggtcg ccatcaaaga acgaaccaaa
      181 ctgcccgtca atatgcacac ccacgccacg agtggcaccg gcgctatgac ctatctcgac
      241 gcggtgcaaa tcggcgcgga tatgattgac accgctattt cgcccctgtc cgacggcacc
      301 agccagcccc caaccgaaac catgcacatt gtgctttcgg agatgaactt cgacaccggc
      361 ctggacggtg acaaactcgt caagattgct tcttacttca aaggtgtccg caagaccttc
      421 cagagtgcca agatttttga tgacaaagtc taccaggtca acccgcttat tttgcaatac
      481 caggtgccgg gtggaatgct ctcgaacctg attgcccagc tcaaagaagt tggcgcggaa
      541 aaccgcctgg atgacgtcct ggcagaagtc gaacgtgtgc gttcagaagt cggctacccg
      601 cctttggtca cgcccatgtc gcaaatcgtt ggcgcgcaag ccgtggtcaa cattttgtcc
      661 ggcagccgaa acaccatcat ccccaacgaa atgcaggact acgtgcgtgg ttattacgga
      721 cgccctgccg cacccatcaa cccggaattt gccgccaaga ttttgcaggg cgaaaagccc
      781 atcacccagc gaccggcaga cttgcttgaa ccccgcttac ccaaagcgcg caaagaaatt
      841 gaacatctgc acccgtctga tgaatctgtc atcagctatg ccatgttccc cgagattacc
      901 aaagcctatc tggaacgcca tcaagtgacc tcagaatacg gatgggcaaa cgccggcgcc
      961 ttgggctaaa caagcttttt ccaaaactta aaacccccac ttgactgaat gggggttttt
     1021 cttttaatgg attgtcacta tttcagcctt tgcagcgact tattaatgta gctacaaatt
     1081 aaaagtgttg aaggatatct atgaaagata caattagagc gaaggttcag ttataggtga
     1141 acagatgaaa agcaaccatc cacttgattt agagcagtca tttgaccagc tttatcgtga
     1201 aaatctgcga cccctctttc aatacctata tagtcgtgtg caagatattc acctggctga
     1261 ggacctatgc tcccaaactt ttatcagcgc gtttgaatcg ctcgaccgtc tcaaatcgcc
     1321 tgataaattc aagccatggc tgtttactat cgcccgtaat aaggcgaatg accattttcg
     1381 taagctcaag cgtcaaccga tgcttgaact tgatgaaatc attgcgacac accccttgca
     1441 ggaagaagca ggaaataaca acaagcaaga ccaacgaatt ctcttggaac agctttttgc
     1501 cagacttaaa cccgcggaac aggaattact gcgcctcaaa ctggttgcgg ggctgacctt
     1561 caaagatatc gccatcctgc taaaaagcaa tgaaaacaat gttaaaaaat cttattatcg
     1621 gctcctggag cgccttcaag cccaggtgga gtaagacaat gaaaacacaa caatttgaag
     1681 aaaaaatcca gtcttctttc gcgttacccg aaccttcgcc tgaattcgaa gaaaggctgg
     1741 cccgaacatt gcgagccaag gttcacgcac agaaaataac accacgtcgt caggttcagg
     1801 taccccgcgc atgggcatat gcatttgcga ctcttgccgc attagtcatc gttgtttttg
     1861 cccttggtcc acaaaaagtg ctggcacaaa ttcaatcctg gttaggtttt ctcccaggta
     1921 taggtttggt gtctgatgat gcttcctcct tgcgcgtgct ggcagaaccc gcttctcaga
     1981 ctttggacgg catcaccgtg gaagtttcag aagctgtggc gagctccgat aaaacctatg
     2041 tgaattatca ggtttttggt ctcaccaacc agcattttcc agcccaggaa agcgacccag
     2101 gctgccattt tgaaccaaac ttgaaacttc ccaacggcac caaccttgcg tttgcaaatg
     2161 gctattatga agcggtacct aaggaaatca accaagttgt gctccatcta gcgtgtatcc
     2221 cgggcacaga ctccgccaaa accccttcca actggcaaat tccctttacg ctcaaaactg
     2281 ctgatgattt agtgaccatg cctgtattac tcccagaacc tcaagtttac agacaatcgg
     2341 atcaaacatt cggtgaagct attgatgact cgtctgcctt gcacattcat acagtggtcg
     2401 agacacccaa aggttacatc atgaccggct gggttgagac agatctaaat atcgcgcttg
     2461 gcccgatgaa tcccaaacta atcgattcaa acggcaagga agttcagacc aggctcagcc
     2521 aacaagccag cgatctgatg caaaacatcc ctgctgaaca ccagttttat gcatggggct
     2581 ttgaattcga cagcgaaggg cttagtttcc cattaaccgt cagctttagt gtgctaccaa
     2641 aggcagacca aaatatgttt gaacccatcc atttcatggt tgatctcggc gcaaatccgc
     2701 aaatgaccag gtatgggaat tccagcagac acttagcctt ggtacagctc aaattaccat
     2761 aaataaggtg caatatctgt cgcaaaaccg cttaaagctt ttctttaccg cgacgaatgg
     2821 gctaattgac cttgatattc aattccctga tttacaggtt tctggtggtg gcggtggtgg
     2881 ccattcggat gcctctgata gctctatgtc ttttgaaaga agctttgtgt ttgagacaac
     2941 gcccaaaggg attgttgaaa tccttttcac cgacccagtt ttcgctcaag acgcagtgga
     3001 cttcagcagt gtttggcaac ctgccacacc tcacgcgccc tatcctgaaa tggcaacgca
     3061 cacctgccca tcattagcca tagacagctc acaaagcaat ttcccagtat ttgaccaggc
     3121 ttacatcgcc ctctatcgcc aaataagtgg taatccggtc aaatatgagc taatttatcg
     3181 taactctcaa gcgacagaag gactggtcct ttcagataat gcggtttggg gctccataag
     3241 cccggacagt acacaagtac tcttcccgaa aggtttggaa ggttccactt tgcaaaacct
     3301 aagcgataat caaaaacaga tactcgctgg tatcatcgct aatgacagca aatggtcacc
     3361 cgatggcaac tatattgcct acctccgggg agacggtgct gtgttcaaca tgcctgcttt
     3421 cgccaaagta gatggaactg aagctaacac aatcagcgaa ctgggttacg ccatgattgc
     3481 aggatggtct cccgacagcc gcacgattta cttcacactt ccctggttta acagttcttc
     3541 gtggaaggtg atggcttatg acattgaaag cgatgaaact aaagaacttt tcaccattca
     3601 aaacggcaca attaaattcc ttgacccgca gatctcgccc gacgggaaat ggttgagtta
     3661 ccgtggcgtg gacaactcaa gcttatattt ggtgagtttg gatggcgaga cggagcgttt
     3721 aatcctggat gatgcccacg ttgtcaaaca catatggctg gatgatacgc atatcgcgct
     3781 ctcaatatcc tatttttcag gcacttacga gaattatgtg ctcaatttga atgattgtag
     3841 cttcatgcgt ctggatgacc tggatggtgg tttggtggat atgcagttaa aaaattaaac
     3901 gtcccctaac aatcgtgtat taaaaaaggc ttattgtgat ggtgcaataa gccttttgaa
     3961 tttttgaaaa atggagagcg attagtcttt caatagctcc agcaaaatct cacgcgttaa
     4021 tttcgggtcg gctttgccac gggttgcgcg catgacctgc cccacaaacc agcccatcag
     4081 cgtttctttg ccaccgcggt aagcggctac ctcatttggc gcgccagcca aaatggtttc
     4141 aatctgcttt cgcagactgt cggcgtctgc aatcaggccc aagccttctt cttcaacaat
     4201 cgtggcgggc gacttgccgg tttgttccac tttgaccagc aaggacttac ccgtcgcggc
     4261 gttaatcttg ccgtcatcaa tcagtttcac gatttcaacc agtgcagccg gcttcaggac
     4321 caaatcccgc gcgctcttgc cggtgtcgtt catcaggcga atcatatcgt tcatcatcca
     4381 gttactgatt gttttgggct ctttggtata caaggcaaca gcttcttcaa agtagtccga
     4441 aagaccacgc tctgaggtta agacctcggc atcataagcg cttaattgat actcttcaac
     4501 aaaacgtctg cgtcgggcat agggtaattc gggcaaactg gctttaatcg cctcaatttc
     4561 ctcattgctc aggatgaccg gctgcaggtc aggctcgcgg aaataacgat aatccgcctc
     4621 gccctctttt gaacgcatca tgctcagttt gccggtgtcc gcat
//
LOCUS       DAOSFC010000094         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_16675_length_4619_cov_4.240359, whole genome shotgun sequence.
ACCESSION   DAOSFC010000094 DAOSFC010000000
VERSION     DAOSFC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16675_length_4619_cov_4.240359"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..890)
                     /gene="lexA"
                     /locus_tag="PK791_03420"
     CDS             complement(183..890)
                     /gene="lexA"
                     /locus_tag="PK791_03420"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOH92397.1"
                     /translation="MARTKRKGLGPRHQKILNFLEEVLQEQGLPPTIREIGDATQISS
                     TSVVNYYLEQLEEGGYIERRSNVSRGITLLKNAAGEALQDIGKAISDAMQTLTVPLLG
                     NIVAGEPMPVPASDFSYYDPESGIDIARSLLPAHESEDQLYALRVHGDSMIDAMINDG
                     DIVVMKKTNQAKNGEMVAIWLNDRDETTLKYFYRENGKVRLQPANPTMQPIIIDDPRI
                     VEVQGKVILVIRQVSEN"
     gene            1137..3680
                     /locus_tag="PK791_03425"
     CDS             1137..3680
                     /locus_tag="PK791_03425"
                     /inference="COORDINATES: protein motif:HMM:NF020498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HOH92398.1"
                     /translation="MKKINRLISVLVTFAVLIVALQSNPAFGQDTGQTATPNTEIAFD
                     DVSTNTQIQFGLIDENHGWVAIDDALYISDNQGETWADITPKQELGVYQKIAIQADGQ
                     GSIISVNLHDESIQATVLKTRNFGEDWQIIDSNLQFVLLNNHAGPIEELFVQWHNADV
                     GWLMAKQVSGINFSHGVLLRTIDGGKYWSALPCPAGERFVFIDTQIGFMHSPAKPEIF
                     YYTSDGGQNWEDFALPTEPLENTLFTVKLPVMLADGGLIVPLELEFEDGSRQVGFFHT
                     ENLLPNFKLEESFELIKSKVSESSNHIHDEELVQALMLNTDTLSFLNKNQGWGLFEGG
                     ECQEQAGKIICRYVRELRSTTNGGESWQLLRLPKQVAGDVRTFSFPTSLDNTVDNQVS
                     TGQVGIYKGQAFDACEIPSLAQLNNWFQHSPYRGVNLYIGGISRLCANSNLNEAYITE
                     IARQGWRLIPTWVGHQPPCTSFKYPFPYDVDEAFEYGVNNANQAKDRMETFGLLNSDG
                     RGGVVYLDVESFNTSNEACVAATRAYIRGWTTRMNELGIMGALYASSINLNKAKIYNL
                     SPAVPAVWIAEWNIARGFNPDASVYDLRHLPNDYWYSEQRLRQYSGEKYETWGGVTIE
                     IDPNVADGPVMALTNLPPSRPVVSITLNGQKGLDDWYKSQVNVFIHAYDYGVGIDKVY
                     RRIDEGPWKEYQPFGVNGSGKKTISYYAVNNAGLQSDIKSISFYVDNQPPINPSITKI
                     GCDAISGIPQARCNDASFEWSGAYDAGVGLDLENTYQVYWGTDPYGTSTDIQNTTYFD
                     PPAIPANTAYFLRIRTQDRHEIWSEWQTLFALIYGPDYQYFNWYAPIRK"
     gene            3799..>4619
                     /locus_tag="PK791_03430"
     CDS             3799..>4619
                     /locus_tag="PK791_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOH92399.1"
                     /translation="MKESTRNQRQKKQIKDVFKKPVWRDVKDYLLVIFGALIQALGTW
                     LFIIPGGLVSGGVFGLAQIIYYKFGIPVGVVAILFNIPLFFLGWKYLGGFRFAIRTVL
                     SIVISSVAIDLLVNFVSQDVAITQEPLLNTLFGGVLVGVGLGMVYLGRGTSGGTDIIA
                     RILNKRMGMSVSTIYMVTDTISVLLAGIFFDWEIALYGLILIYVSGVAVDMTLQGIDV
                     VRQAMIVTDNYEAVANAIVEELDRSSTIIPAKGGYTKMERNILFCVVMRSEVARL"
BASE COUNT         1241 a          918 c         1117 g         1343 t
ORIGIN      
        1 cgaagttgtg cttgcataag gccgacttaa tcgaaacaac aaaaagaccc cgagcaatcg
       61 gggtcttttt gtgtatccaa aaacaggctt tttaaagaga aagaatggct tgtaataatc
      121 tctttaactt tgttgatatt taaggagtaa atcaaatagt aatcgtctgt aacagcgtag
      181 catcagtttt ccgacacctg gcggatgacc aggatgactt tgccttgtac ttccacaatg
      241 cgcgggtcgt cgatgatgat gggctgcatg gtaggattgg caggttgcag gcgcactttg
      301 ccgttttcgc ggtagaagta ctttagcgtg gtttcgtcgc ggtcgttgag ccaaatggca
      361 accatctcgc catttttggc ttggttggtt ttcttcatga ccacgatatc gccgtcatta
      421 atcatggcat caatcatcga atcaccatgc acgcgcaagg cgtagagctg gtcttcagat
      481 tcgtgcgcgg gcaggagact gcgggcgatg tcgatgcctg attcagggtc ataatagcta
      541 aaatcagagg caggcacggg cataggttcg ccggcaacaa tattacctaa aagcgggaca
      601 gtgagcgttt gcatggcgtc gctgatcgcc ttgccgatgt cctgcaaggc ttcaccggca
      661 gcgtttttga gcagcgtaat accccgcgat acgttggaac gccgttcgat gtagccacct
      721 tcttcaagtt gttccaggta gtaattgacg acagaagtgg atgatatctg tgtggcatcg
      781 cctatctcac ggatggtggg gggcaatcct tgttcctgga gaacttcttc gagaaaattc
      841 aaaatttttt gatgtcgtgg gccaaggcct ttcctttttg ttcttgccat atcgcctcgc
      901 aatccaataa aagaactaaa tatgattatc tagaatagta tcagaacata tgttcactgt
      961 caagggtgtt gcttcgttgg gctaatgtaa acaatttgtc aaacctttta aaaatagact
     1021 ttaataattc tgctatgatt aaaaatgtcc caatgtgata aactaagggc atgaatattg
     1081 caccctatct actggatatt attaattttg ttttacaatt atagtgtgag aatgttatga
     1141 agaaaatcaa tcgattaata tctgttcttg tcacttttgc tgtcctaatt gttgcgttgc
     1201 aaagcaatcc tgcttttggc caggatacgg gccagacagc aacgccgaac acagagattg
     1261 cgtttgatga tgtcagcaca aatacgcaaa tccaatttgg cttgattgac gagaaccatg
     1321 gctgggtggc tattgatgac gcgctttata taagtgataa tcaaggtgaa acctgggcgg
     1381 atattacacc caagcaagaa ctgggcgtat atcaaaagat agcaattcag gcggatggcc
     1441 agggtagtat tatttcggtc aatcttcatg atgaatctat tcaagctaca gtgttaaaaa
     1501 cgcgtaattt tggcgaagac tggcaaataa ttgacagcaa cttacagttt gtgctattga
     1561 acaatcatgc gggccccata gaagaacttt ttgtgcaatg gcacaatgcg gatgtgggct
     1621 ggttgatggc gaaacaagtt agtggcatta attttagtca tggtgtgtta ctaagaacga
     1681 ttgacggtgg taaatattgg agcgcattgc cctgcccagc tggcgaacgc ttcgtcttta
     1741 ttgacactca aattggcttt atgcattcac cagcaaaacc ggaaatcttt tactatacga
     1801 gcgatggcgg acaaaattgg gaggattttg ctttgccaac cgaacctctg gaaaacactc
     1861 tttttaccgt aaaactgccg gtaatgctcg ctgatggtgg cttaattgta cccctggaac
     1921 tcgaattcga agatggcagt cgtcaagttg gcttcttcca cacagagaac ttattgccga
     1981 attttaagct tgaagaatcg tttgaattaa ttaaatctaa agtctctgaa tcaagcaatc
     2041 atattcacga tgaagagttg gttcaggcat taatgctcaa cacggacaca ctcagtttcc
     2101 taaacaaaaa tcagggatgg ggtttgtttg aaggcggtga gtgtcaggaa caagccggaa
     2161 aaattatttg tcgttacgtt agagaactac gaagcaccac aaatggtggg gaaagctggc
     2221 aactattgcg cttacccaaa caagtggccg gcgatgtaag aactttcagc tttccgacat
     2281 ctctagataa tacagtggac aatcaggtat caaccggcca ggtcggcatt tataaggggc
     2341 aggcctttga tgcctgcgaa attcccagct tagcccagtt aaacaattgg ttccaacaca
     2401 gcccctatcg gggtgtaaac ctatatattg gtggtatttc acgtttgtgt gccaacagta
     2461 acttgaatga ggcatatatt accgagattg cccgtcaagg ttggcgttta attccgactt
     2521 gggtgggaca tcaacctcct tgtactagtt tcaagtatcc tttcccatat gatgttgatg
     2581 aggcttttga atatggcgtt aataatgcca atcaagcaaa agaccgcatg gaaacattcg
     2641 gtttgctgaa tagcgatggc aggggcggtg tggtgtattt agacgtagaa agtttcaaca
     2701 cttcaaacga agcttgtgtt gcagcaacaa gggcatatat tcggggctgg acaacacgta
     2761 tgaatgaact gggcattatg ggtgccttgt atgctagttc gattaatcta aacaaagcca
     2821 aaatttataa tctttctcct gctgtccccg cggtttggat tgccgaatgg aatattgccc
     2881 gtggttttaa tcctgatgct tcagtttatg atttgcgtca tttgcctaat gactattggt
     2941 atagtgagca acgcttacgc caatattctg gtgagaaata cgaaacgtgg ggtggtgtaa
     3001 ccatagaaat tgatccaaat gtggctgatg gccctgtaat ggctttgacc aatttacctc
     3061 catcaaggcc ggttgtatca ataacactca atggtcaaaa ggggcttgac gattggtaca
     3121 aatcccaagt gaacgtgttt attcatgctt atgattacgg tgtcggcata gataaagtct
     3181 accgaagaat tgatgagggt ccctggaaag aataccagcc ctttggtgtc aatggttccg
     3241 ggaaaaagac aataagctat tacgcggtga acaatgctgg cttgcagtcg gacattaagt
     3301 cgattagctt ttatgtggat aatcagccgc caataaatcc atctatcaca aaaattggct
     3361 gtgatgcgat tagtgggatt ccgcaggcac ggtgcaatga tgcctcattt gagtggagcg
     3421 gtgcttatga tgctggtgtg ggtttagacc ttgagaatac ctaccaagta tattggggca
     3481 ctgatcctta tggtaccagc accgacatcc aaaacacgac atatttcgac ccaccagcca
     3541 tacccgccaa tacagcttat tttttacgaa ttcgcacgca agaccgacat gaaatttgga
     3601 gtgaatggca aacgctcttt gcgcttattt atggcccgga ttatcaatat tttaactggt
     3661 atgcacccat aagaaaataa aattttattt acgtggttta ctttcaaatc tggtgtaaaa
     3721 tcgcagttga attggtgcaa attgctcaaa ttgagatatc atatgagtca taatcatgcg
     3781 tatggataag gtgtgtttgt gaaagaaagt actcgaaacc aaagacagaa aaaacaaatc
     3841 aaagacgtat tcaaaaaacc ggtttggcgg gatgttaaag actaccttct cgtcattttt
     3901 ggtgccttga ttcaagcgct tggcacgtgg ctttttatca ttcctggcgg attggtaagt
     3961 ggtggtgtgt ttggtttggc gcaaatcatt tattacaaat ttggtatccc ggtgggtgtt
     4021 gtggctatct tgtttaacat accgttgttt tttcttggct ggaaatacct tggcggattt
     4081 aggttcgcaa ttcgcaccgt tttgtcgatt gttatttctt ctgttgccat tgacctcctg
     4141 gtaaattttg tttcccagga tgttgcaatc actcaggagc cattgctcaa caccttgttt
     4201 ggtggtgttt tggtgggtgt tggtttgggt atggtctatt tgggacgtgg taccagcggc
     4261 ggtacggata tcatcgcgcg cattctcaac aaacgcatgg gtatgtcagt ctcgaccatc
     4321 tacatggtaa cggacaccat ttcagttcta ttggctggta ttttctttga ctgggaaatt
     4381 gcgctctacg ggctcatctt gatttatgtt tcaggtgtgg cggtggacat gaccctgcaa
     4441 ggtatcgacg ttgtgcgcca ggcgatgata gtgaccgata attacgaagc ggttgccaac
     4501 gcgattgtcg aagaacttga tcgcagctca actattatcc ccgccaaagg tggctatacc
     4561 aaaatggaac gcaatattct cttttgcgtg gtgatgcgct cggaagtggc acggttgaa
//
LOCUS       DAOSFC010000095         4610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_16723_length_4610_cov_3.706476, whole genome shotgun sequence.
ACCESSION   DAOSFC010000095 DAOSFC010000000
VERSION     DAOSFC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16723_length_4610_cov_3.706476"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2127
                     /locus_tag="PK791_03435"
     CDS             <1..2127
                     /locus_tag="PK791_03435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOH92400.1"
                     /translation="AKLVMESDLAYDQEVLIVTFSNVAADNFTSRIGAILSEQGIVEG
                     IGYKVRTLHGLASDIIRERPDLAGLGNDFSIIDASESTEILQDLIRNQMMQKPEIFNE
                     LVSDNYRSRNNDAKIEKELLTELHRIVTVYIKTAKDHLLSVEELQTHINKTESPTSLL
                     LICQEIFRSYQAALNYRGAVDFDDLIRLAWLSLNNDADLLKSLQYRWPYILEDEAQDS
                     SLVQEAILGLLTKTHQNWVRVGDPNQAINESFTTANPQLLRNFIARPDVRSETLPESG
                     RSCPAIIALANNLNEWTQHHHPNPYLRNALSHPLIELTKPDDPQQNPKCATPSVYFNT
                     QRFNTQEEMDFIIEDVKAWLEEHPEDTVAVLALSNDRVGRLAGALTKAEIPVEQALMK
                     NPINTRKSAGSIFFILRALVNPTDTNALAQAFKVFYRHYQDDEESWLVVEKCMRYIQN
                     TDHLEYLIYKENFDQPIVFEDMLEEELAHDFLTRFIFKLRRWHQATILPLDQTIITIA
                     QDLMLDPIELATVHKISQLVRQILQDQPEWSINNVLSEIKDIANNTKTFLSFNEAEDG
                     FNPDLHKGKVVVATYHKAKGLEWDKVYLSSLNNFDFPSASPDDYFLSERDIFVEPKNR
                     QAEIKMELEAIINPNAPAYEPGLATIIDRNDVSRERIRLLYVGITRAKKSITASYNTG
                     RFLKQDEALASRELRKFLTDENTSTP"
     gene            2105..2917
                     /locus_tag="PK791_03440"
     CDS             2105..2917
                     /locus_tag="PK791_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOH92401.1"
                     /translation="MKTQVPHNFVFSQSNLHTYLNCRYKFYLRYIQKLAWPAQTTIDD
                     LRFEEDRNAGIRFHQLLHQYFIGFNPELLIRMAENDPDSRVLVWFQTFLASPYAQLQG
                     KLESEKTITTCVDDKNFIVKYDLLQFKSGQYTIYDWKTSNKVPNRERLIDNIQTRLYP
                     VILSEAYQTDLPIRLIIWEIIAPDAPFIFSFSSQELKRNAQYFKSLCDEIVSLEKTDF
                     FKTANTRQCKICEYRSHCNRGIAPATYEYLEESAFTDLEDYWNTNRDDANLV"
     gene            complement(2933..3006)
                     /locus_tag="PK791_03445"
     tRNA            complement(2933..3006)
                     /locus_tag="PK791_03445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2970..2972),aa:Val,seq:tac)
     gene            3030..3797
                     /locus_tag="PK791_03450"
     CDS             3030..3797
                     /locus_tag="PK791_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOH92402.1"
                     /translation="MRIARFISQDKTIQYGWIDEDRIGTIQGNPFGEYRRFEAKQKLN
                     EVKLLAPCEPSKIIVVAHNFAYKSEKELTPPPDYPIFSFKPSSSVISSGEPILLPPQS
                     QRVEHEAELAVVIAKKARWLRQEDVQDAIFGYTIANDVTARDLEIRDGHSSRAKGFDT
                     FCPLGPWIETKFDPSDAIISCNVNSTMRQMASTRDMHFTIDRLLMFISSVMTLMPGDI
                     VLTGTPNGSSTLEAGDVVTVNITGIGNLVNPVRVAGS"
     gene            3803..4090
                     /locus_tag="PK791_03455"
     CDS             3803..4090
                     /locus_tag="PK791_03455"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOH92403.1"
                     /translation="MPPLFILTLKFRLQGCSSLKEKRSRMASFLAKLKKENISVIESD
                     FLNQHQESEITIAKLSINNSALNRDLDQLLAFIDYHRGDIELYDYQVERYN"
     gene            4103..>4610
                     /locus_tag="PK791_03460"
     CDS             4103..>4610
                     /locus_tag="PK791_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOH92404.1"
                     /translation="MTLIDKKLLEKILYHVEKPGRYVGGEHNMVLKDWQSVETRVALA
                     FPDIYDLGQANLGLSILYELINDHPNILAERVFSPWHDMEDALREHHIPLFSLESKTP
                     IKDFDILGITLPYESIYTNVLNLLDLSGIPLRSEQREAHHPLVIAGGQACFNPEPMAD
                     FIDAFAIGD"
BASE COUNT         1394 a         1096 c          891 g         1229 t
ORIGIN      
        1 gccaaactgg tcatggaaag cgatttggct tatgaccagg aagtgttgat tgttaccttc
       61 tcgaatgtcg ccgcggataa ctttacatcg cggataggcg caattttatc cgaacaaggc
      121 atcgtcgaag gaataggtta caaagtgcgc accttacatg gactggcaag cgatatcatt
      181 cgcgaaaggc ctgacctggc tggtttaggc aacgactttt caatcattga tgcttctgaa
      241 agcaccgaaa tattacaaga tcttattcgc aaccagatga tgcaaaagcc agaaatcttt
      301 aacgaattag tctctgataa ttaccgaagc agaaacaatg acgcaaagat agaaaaagaa
      361 cttcttacag aattacaccg cattgtgaca gtttatatca aaacggccaa agaccatctc
      421 ctgtcggttg aagagcttca aacgcatatc aacaaaaccg aatcgcccac atcactgctt
      481 ttgatttgcc aggaaatctt ccgttcctac caagccgccc ttaattaccg tggcgcggtc
      541 gattttgacg atttaatccg acttgcctgg cttagcctga acaatgacgc ggacttgcta
      601 aaatccctgc aatatcgctg gccatacatc cttgaagacg aagcccagga ttccagtctc
      661 gtgcaggaag ccattttagg cttacttact aaaacacatc aaaactgggt gcgtgttggt
      721 gaccctaatc aggcaatcaa tgaaagcttt acaactgcca acccacaact actcagaaac
      781 tttattgcac gccctgatgt acgcagtgaa accctgcccg agtcaggtcg ttcctgccct
      841 gccatcatcg cccttgccaa taacttgaat gaatggactc aacatcatca tcctaaccct
      901 tatctgcgca atgcgttaag ccatccgctg attgaattaa ccaaacccga cgaccctcaa
      961 caaaatccta aatgcgcaac cccgtcggtt tatttcaaca cgcaacgctt caacacccag
     1021 gaagaaatgg atttcatcat tgaagacgta aaagcctggc ttgaagaaca tcctgaagat
     1081 accgtagcag tattagccct tagcaacgac cgtgtcggcc ggcttgcagg cgcattaacg
     1141 aaggctgaaa tacctgttga acaagcctta atgaaaaacc ccatcaatac gcgcaaatca
     1201 gcaggatcta tctttttcat tcttcgggca cttgtaaacc ctaccgatac aaacgctttg
     1261 gcacaggctt tcaaagtttt ctaccggcat taccaagatg acgaagaaag ttggctggtt
     1321 gttgaaaagt gtatgcgtta cattcaaaac accgaccatc ttgaatactt gatatacaaa
     1381 gaaaactttg accagcccat cgtttttgaa gatatgcttg aagaagaact ggcacatgat
     1441 tttcttacac gctttatttt caagctaaga agatggcatc aggctaccat attgcccctg
     1501 gaccaaacca ttatcaccat cgcgcaagac cttatgctgg accccataga acttgccaca
     1561 gttcataaga tatcccaact ggttcgtcaa attttgcagg accaacccga atggagtatt
     1621 aacaacgttt tgtctgaaat aaaagatatc gccaacaata ccaaaacctt tctcagtttt
     1681 aacgaagctg aagatggttt taaccccgac ttacataaag gcaaagtcgt tgtggccaca
     1741 tatcacaagg caaaaggttt ggaatgggat aaagtctatt taagttctct caacaatttt
     1801 gattttccat ccgccagccc tgatgattat tttctttcag aacgggatat cttcgtcgaa
     1861 cctaaaaacc gccaggcaga aataaaaatg gaactggaag cgatcatcaa ccctaacgcg
     1921 cccgcatatg agccaggcct tgctacaatc attgacagaa acgatgtttc ccgcgaacgc
     1981 atccgccttt tgtatgtagg gattacccgt gctaagaaat caatcaccgc aagctacaat
     2041 acgggaaggt ttcttaaaca agatgaggcc ttggccagcc gggaattaag gaagttttta
     2101 acagatgaaa acacaagtac cccataattt tgtcttttca caaagcaatc tgcatactta
     2161 tttgaattgt cgctataagt tttacttgcg ctatattcag aaacttgcct ggccagccca
     2221 gacgaccata gacgatttgc gttttgaaga agaccgtaat gcgggtatcc gctttcatca
     2281 gcttctgcat caatacttta tcggtttcaa ccccgaatta cttatccgaa tggctgaaaa
     2341 tgaccctgac agtcgcgttt tagtttggtt ccaaactttt ctggccagcc cctacgcgca
     2401 acttcaagga aagcttgaat cagagaaaac cataacaacg tgtgtcgatg ataaaaactt
     2461 catcgttaaa tatgacctat tacaatttaa aagtggacaa tacacgatat atgactggaa
     2521 aacatctaac aaagtcccta atcgggaaag attaatagat aatatccaaa cccgacttta
     2581 tcctgttatc ctttctgaag cttatcagac cgacctacct atccgactga ttatttggga
     2641 aattattgcg cctgatgcac cattcatttt ttctttttca tctcaagaac tcaagcgaaa
     2701 cgcccaatat ttcaaaagtc tttgcgatga aatagtttcc ctggaaaaaa cggacttttt
     2761 caaaactgcc aatacccgtc agtgtaaaat ctgcgaatac cgttcccatt gcaaccgtgg
     2821 cattgcacca gccacgtatg aataccttga agaaagcgct tttactgacc ttgaagatta
     2881 ctggaataca aacagggatg atgcaaactt ggtataattt gcagagaaaa aatgggcaga
     2941 acaggactcg aacctgcgac ctcttctgtg taaaagaaac gctctaacca actgagctat
     3001 ccgcccggga agacctatta tacatcaaaa tgagaattgc aagatttatt tcacaagata
     3061 agaccattca atatggttgg attgatgaag accgcatcgg aaccatccaa ggtaaccctt
     3121 ttggcgagta tcgacgcttt gaagccaagc aaaaactcaa tgaagtaaaa ctccttgccc
     3181 cttgcgaacc cagtaaaatt atcgtcgtcg cgcataactt tgcatataag tctgaaaaag
     3241 aattaactcc cccgccagat tatcctattt tttccttcaa acccagttca tctgtaattt
     3301 ccagcggtga gccaatttta cttcctccac aatcgcaacg ggttgaacat gaagcagaat
     3361 tagcggttgt cattgctaaa aaagcgcgct ggcttagaca ggaagatgtg caagatgcca
     3421 ttttcggtta caccattgcc aatgatgtta ccgcccgtga ccttgaaatc cgtgatggac
     3481 actcgagccg cgccaagggt tttgatactt tctgcccact tggaccctgg attgagacta
     3541 aatttgaccc atctgatgca attatttcct gtaacgtcaa ttcaaccatg cgccaaatgg
     3601 catcaacgcg agatatgcac tttacgattg accgcctttt aatgtttatc agttcagtca
     3661 tgactctcat gccaggggat atcgtcctta ccggtacgcc taatggcagc agcaccctgg
     3721 aagcgggcga tgttgtaacc gtaaatatta ctggtatcgg caacctggtt aacccagttc
     3781 gtgtggcggg aagttaaaca atatgccacc cctttttatt ctcacactaa agtttcgtct
     3841 gcaaggttgt tcaagtctta aagaaaaacg gtcgcggatg gcgtcatttc ttgccaaact
     3901 caagaaagag aacatttcag ttattgaatc tgatttctta aatcaacatc aggaatctga
     3961 aataactatc gctaagttga gcatcaataa ctctgcctta aaccgcgatt tagaccaact
     4021 tttagcattt atagattatc atcgtggcga tattgaactt tatgattatc aagttgaacg
     4081 ctataactaa taaggattac ttgtgacact tattgataaa aagttgttag aaaaaatttt
     4141 gtaccatgtt gaaaagcctg ggcgctatgt cggtggtgaa cacaatatgg ttttaaaaga
     4201 ctggcagtct gtcgaaaccc gcgttgccct ggcttttccg gacatctatg acctgggtca
     4261 ggctaacctg ggactaagca tcttatacga actcattaat gaccatccta atattttggc
     4321 agaacgggtt ttttcaccct ggcatgatat ggaagatgct ttacgggagc atcacatccc
     4381 tttgttttcc ctcgaatcca aaacgcctat aaaagacttt gatatccttg gcattaccct
     4441 tccatacgaa tccatctaca ccaacgtgtt aaatctcttg gatttatcgg gtattccact
     4501 aaggtcagaa caaagagaag cccatcatcc cctggtcatc gcaggcggac aagcctgttt
     4561 caatccagag cccatggcag atttcattga tgcgtttgcc atcggtgatg
//
LOCUS       DAOSFC010000096         4583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_16880_length_4583_cov_5.105345, whole genome shotgun sequence.
ACCESSION   DAOSFC010000096 DAOSFC010000000
VERSION     DAOSFC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16880_length_4583_cov_5.105345"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PK791_03465"
     CDS             complement(<1..831)
                     /locus_tag="PK791_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOH92405.1"
                     /translation="MEIVIVGGGKLGSALCQDLANEGHEIVVIDQNQDKIDSLIENID
                     IRGVQGNGSTIEIQKEADVPRADVFIAVTPNDETNLIAATTANRLGAKFTIARVRNPE
                     YARDLSFYQKELGIGMLINPDAEAAADITRAFDYPAALTIEPFAGGMVDIVQIRVNPG
                     SAITGRNLAEIRAEIPELLLCAIDRDFETIIPKGNTTLEEHDTIQMVGRPKAIHQFSR
                     LCGHKNERWRTALIVGGSRIAYYLIPDLLKRGVRVKLIEYDATVAEHFAINFPDAEII
                     I"
     gene            977..1810
                     /locus_tag="PK791_03470"
     CDS             977..1810
                     /locus_tag="PK791_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH92406.1"
                     /translation="MELYEKTVLITGATDGMGKETALMFAKVGATVLIHGRNPKKLAD
                     TLAALREANPKGQYYSYQADYASLADVRAMAEAIKKEHQQIDILINNAGLYPDKRVIT
                     QDGFELTLQVNYLSSFLLTHALMPLLEASPEARIVNVSSIGHKLVWNSIKNPKFSPFF
                     WRWVSYCRSKLLVVAWTIELARHLESKKITVNSVHPGVIRTKVIRILPVSWGSSVPSG
                     AKTIFNLATNPAYATTSGQYFERYKLAKAAPMALNKRLQKNLWRASHDWAGLVIESEH
                     E"
     gene            1803..3023
                     /locus_tag="PK791_03475"
     CDS             1803..3023
                     /locus_tag="PK791_03475"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HOH92407.1"
                     /translation="MNKRRFCKAKTLLLIISIMSVVLSACQNTPPMLTPVFNGVNTPV
                     VKTAEMATRLAEHATPTPVPSATPWPVASSTPKSDIIQSTHGFVNPLTGLYVDKPALL
                     RERPILVKIANWPENLRPADGLNKADMVFEYFIGAQTNHLVALYYGDDHEHVGPLGPG
                     RVVDARLTEHMQGNLVLASADSVSESVLEDFLPHRYTMRGFAPCPGICTETQAQGGNT
                     FVDTSAIRAFLEKESASNFTPSLFGLAFSETAPNSNDQALRFSYMYADFSVMDWRFDE
                     EQGNYQLWQDRQQEPGHYTLTKSEDRGTGEPIAFENVIFLFTNYVNYRDAFYDINFKD
                     GNTEQRGLLLRDGYLYHIRWSAADYDQPFLFYDLDGEPFQLKPGKSWITLSTLDSRLE
                     RLDDGAWDMQFIIK"
     gene            complement(3070..>4583)
                     /locus_tag="PK791_03480"
     CDS             complement(3070..>4583)
                     /locus_tag="PK791_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOH92408.1"
                     /translation="IGDRIIVRAGDRLPMDGIIISGESELNQAAITGESAWVAKQAGD
                     EVLSGTINGSGLLEVQVTRLVEDTTMQRIIRLVTEAQASKAKQEKFIDKFASIYTPIV
                     MLIAILVVVIPTVFFRQALWNTPHAYGWLHRGLSLLMIGCPCALVISTPVTIISGLTR
                     AARSGVVFKGGVYLEGLSKVKAMAFDKTGTLTRGEPKVELVKAVDCENSDEPCESCDN
                     LLALASALEERSHHPLSSAILEHAEDRGVRGKIAPAEDLKVLAGRGQEGTVNGKKATV
                     GSLPLFLAEHRTPAKLVDETRQAEADGQTTVLVCDGDSVRGYLSLRDQARPEATEVLN
                     ALQDLNVHTVMLTGDNAGAAQNIASQLGIHEVKSSLLPHEKLDALELLQETFGTVAMV
                     GDGINDGPALARADIGVAMGGAASAQILETADVVLMNNDLKRLPFALRLSRFTNRLIR
                     QNILVSLGVKFAVAILAILGLTPLWVAVLADIGIALAVTLNGLRATSFEKETI"
BASE COUNT         1099 a         1108 c         1206 g         1170 t
ORIGIN      
        1 gataataatt tctgcatctg gaaaattgat agcgaaatgc tcagcgacgg tagcgtcata
       61 ttcaatcaat ttaacgcgga caccacgctt tagtaagtcg ggaatgagat aataagcaat
      121 gcgacttcca ccgacaatca aagccgtacg ccagcgttcg tttttgtgtc cacaaaggcg
      181 gctaaactga tgaatagctt taggccggcc aaccatttgg atggtatcgt gttcttccag
      241 ggtcgtattc cctttgggga taatcgtttc aaaatcacgg tcgatggcac aaagcaaaag
      301 ctcaggaatt tccgcacgaa tttctgccag gttgcgtcca gtaatagcag agccggggtt
      361 gaccctaatc tgcacgatgt ccaccatacc ccctgcaaaa ggctcaatgg ttagggcggc
      421 ggggtagtca aaggcgcggg tgatgtctgc ggcagcttcc gcgtcgggat tgatgagcat
      481 gccaatgcca agttcttttt ggtaaaagct taaatcacgc gcgtattcag gattgcgcac
      541 ccgcgcgatg gtgaacttag cacccaggcg attggcggtt gtggcggcaa tgagattggt
      601 ttcgtcattt ggagtaaccg cgatgaaaac gtccgcgcga gggacatcag cttccttttg
      661 gatttcgatc gtggagccgt tgccttgcac gccacgaata tcaatgtttt caatcagcga
      721 atcgattttg tcttggtttt ggtcaatcac gacaatctcg tgaccttcgt tcgccaaatc
      781 ctgacaaagc gcgctgccta attttcctcc accgacgata acaatttcca taagttttcc
      841 ttccgattaa attttatcaa agttttggta tcatgtttat tttttaggta aaaaaattta
      901 ttgtatttga cgtgtttgga tgatgtgtct ataatttttg aaggaatgaa aagagaagga
      961 ctctaagcga gaaaaaatgg aactttacga aaaaacagta ttgattactg gcgcgacgga
     1021 tggcatgggc aaagaaaccg cgctgatgtt tgccaaagtt ggcgcgacgg tgctcatcca
     1081 tgggcgcaat ccaaagaaac tggcggatac gcttgcagca ttgcgggaag cgaaccccaa
     1141 ggggcagtat tacagctatc aggctgatta tgcgtcgctg gccgatgtgc gcgcgatggc
     1201 tgaagccatc aaaaaagagc atcagcaaat tgatattctc atcaataacg ctggccttta
     1261 ccctgataaa agggttatca cgcaagatgg ttttgaactg actttgcagg tgaactatct
     1321 atcttctttt ttgctgacac atgcgttgat gcctttgctg gaagccagtc ccgaagcgcg
     1381 tattgtcaac gtgagttcca tcgggcataa gctggtttgg aacagtatca aaaacccgaa
     1441 gtttagtcct ttcttttggc gctgggtgag ttattgccgc tcgaaattgc ttgtcgtggc
     1501 atggacgatt gaactggcac gccatctgga gagcaaaaaa atcacggtga acagtgtgca
     1561 tcctggcgta attcgcacca aggtcattcg tattttgccc gtttcctggg gctcttcagt
     1621 accatctggt gcgaagacga tttttaattt agccacaaat cccgcatatg ccacgacaag
     1681 cgggcagtat tttgaacgct ataaactggc caaggccgct ccgatggcgt tgaacaaaag
     1741 gctgcaaaag aatctatggc gcgccagtca tgattgggcc gggctcgtga tagaatcaga
     1801 gcatgaataa gcgccgattc tgcaaggcta aaacgcttct gctgattatt tcaataatga
     1861 gtgtcgtttt gagcgcgtgt caaaacacac cgccgatgct gacacctgtt tttaacggtg
     1921 tgaacacacc ggtagtgaaa accgcagaga tggcaactcg tctggcggag catgccacgc
     1981 caacgccagt gccttcggcg acaccgtggc cagtggcgag tagcacgcct aaatcggaca
     2041 ttatccaaag cacgcatggt tttgtaaacc cgcttacggg tttgtatgtc gataagccgg
     2101 ctttgctccg cgaacgcccg atactggtga aaattgccaa ctggccggag aatttgcgac
     2161 ctgcggatgg cttaaacaag gctgacatgg tctttgaata cttcatcggc gcgcaaacga
     2221 accatctggt ggcactttat tatggtgatg accacgagca cgtaggacct ttggggccgg
     2281 ggcgtgtggt tgatgcgcgc cttaccgaac atatgcaagg gaatttggtc cttgccagcg
     2341 cggatagcgt ttctgagagc gttttggagg attttcttcc acaccgctat accatgcggg
     2401 gtttcgcgcc atgtccgggc atttgcacgg aaacccaggc gcaggggggc aatacttttg
     2461 ttgataccag cgccattcgc gcgtttcttg aaaaagaatc agcttcgaat tttacaccca
     2521 gcttgtttgg tctggcattt tcagagacag ctccgaactc aaacgaccag gcactcaggt
     2581 tcagttatat gtatgctgat ttctcagtta tggactggcg ttttgacgaa gagcaaggca
     2641 attatcagct ttggcaagac cgccagcaag aaccaggcca ctatacattg acgaaaagcg
     2701 aagaccgggg cacgggtgaa cccatcgcgt ttgagaacgt catttttctg ttcacaaact
     2761 atgtcaatta ccgggatgct ttttacgaca tcaattttaa agatggcaat accgagcaac
     2821 gtggtctttt gttgcgggat ggctatttgt atcacattcg ctggagtgcc gcggattatg
     2881 accagccctt ccttttttac gatttggacg gagagccgtt ccagctaaaa ccaggcaaaa
     2941 gctggattac cttatccact ctcgacagtc gtcttgagcg acttgatgat ggagcgtggg
     3001 atatgcagtt catcataaaa taaagcgctt ttcggcactt tattttttat cgttcctgaa
     3061 ggtttttagc taaatagttt ctttttcaaa gcttgtcgcg cgcaaaccgt tcaaggttac
     3121 cgccagggcg atgccgatat cggccagcac cgctacccat aatggcgtta atcccaagat
     3181 ggccaggatc gccacagcga attttacacc caggctgact aagatatttt ggcgaatgag
     3241 cctgttagtg aaacgcgaaa ggcgcaaggc aaagggcaag cgtttcaggt cattgttcat
     3301 cagaacgacg tcggctgttt ccagaatctg agcactcgcc gcgcccccca tggccacacc
     3361 gatatccgca cgtgccaggg cggggccgtc attgatgcca tcgccgacca tagctaccgt
     3421 gccaaaagtc tcttgcaaaa gttcaagcgc gtcaagtttt tcgtggggta aaagagatga
     3481 tttgacttca tgaattccca gttggctggc gatattttgc gccgcgccag cgttatcgcc
     3541 ggtgagcata acggtatgca cgttcaggtc ttgcagggcg tttaggacct cggtggcttc
     3601 agggcgggcc tggtctctca ggctcaggta gccacgcacg gagtcgccgt cgcaaaccaa
     3661 aacggtcgtt tgcccatcgg cttcagcctg gcgtgtttcg tccacaagtt tggcgggcgt
     3721 tctgtgttcc gcgagaaaga ggggtaaact gccaaccgtt gcttttttgc cattcaccgt
     3781 gccttcctgt ccacgtccag ccagcacttt taggtcttca gcgggcgcga ttttaccgcg
     3841 cacgccacgg tcttcagcgt gttccaaaat ggcactgctc aggggatgat ggctgcgttc
     3901 ttccaaagca ctggccagtg ctagcagatt gtcacaggac tcgcagggtt catcgctgtt
     3961 ttcgcaatca acggctttga ccaactctac cttgggttca ccgcgggtta aggtgccggt
     4021 tttgtcgaat gccatggctt tgactttgga aagtccctcc aaataaacac cacctttaaa
     4081 aacgacgccg gaacgcgccg cgcgggttaa gccagagata atggttaccg gtgtgctgat
     4141 aaccagcgcg caagggcagc caatcatcag caaggaaagc ccgcgatgca accagccata
     4201 ggcgtgtggt gtgttccaca gggcttgtct aaagaacacg gttgggatga ctacgacgag
     4261 aatggcgata agcatgacga tgggcgtgta gatactggcg aatttgtcga tgaatttttc
     4321 ctgtttggct ttgcttgcct gcgcttcggt cacgaggcga atgatgcgtt gcatggtggt
     4381 atcttcaact aagcgggtga cttggacttc caatagcccg ctgccattga tggtgcccga
     4441 aagcacttca tcgccagctt gtttggcaac ccaggcactt tcgccggtaa tggctgcctg
     4501 gttgagttcg ctttctccgc tgatgatgat gccatccatg ggcaggcggt cgccagctcg
     4561 aacgatgatg cggtcgccaa ttt
//
LOCUS       DAOSFC010000097         4583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_16881_length_4583_cov_4.422482, whole genome shotgun sequence.
ACCESSION   DAOSFC010000097 DAOSFC010000000
VERSION     DAOSFC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16881_length_4583_cov_4.422482"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1849)
                     /locus_tag="PK791_03485"
     CDS             complement(224..1849)
                     /locus_tag="PK791_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOH92409.1"
                     /translation="MLDEQIRKAWRKKRTYTEEELVLARKVLEAVRDGQEVRNAQRMY
                     PLPGGAGFLAKHALVAAYRRLVEQEIWEEDPVLLAKIRMKPIRTLSGVTTLTVLTKPH
                     PCPGECVFCPTIEGMPQSYLPDEPGARRGLENEFDPYRQVASRLKALHDVGHPTEKIE
                     LLILGGSWSAYPKDYREWFLRRCFEAMNGENPLDDQGDVPLYEVQARNVNAEKRNVGL
                     VIETRPDLINAEELKLLRKQGVTKIQIGIQSMNDEILEKNKRGHDSATTLKATALLRE
                     AGFKITAHWIPNLLGSTPDLDKEDFKRLWEESGVNPDELKIYPCQLLNSAPLYGIWQD
                     GDYEPYDETTLLNLLADIKTMVPRYCRINRVIRDIPSTNVIEGSRNTSLRQDVAVEMA
                     RRGTKCECVRCREIREQRMEAGELVYDDFSYQAAGAEEHFLSYNTADDKLAGFLRLSL
                     PNDDNALDIDDLKDAAIIREVHVYGQSLEVGTEQEGMAQHAGIGKSLIAHAERIAKDH
                     GYQKIAVIAAVGTRAYYAGRGFELGELYMVKPV"
     gene            1960..2715
                     /locus_tag="PK791_03490"
     CDS             1960..2715
                     /locus_tag="PK791_03490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92410.1"
                     /translation="MPKMRPETSQKLLALNKSFYENNAQSFSSTRYAIQPGVRSLLPS
                     MLTARTILDLGCGNGNLALALDKAGFCHEYFGVDNSQCLIDDAISARDKRIAKRAHYT
                     FQVLDLGQTPWQIAADRRQFDMIVSFATLHHIPGLRLQQEFFMQAASLLETNGTFIIS
                     CWQPLNSPRLAKRVQNWDVLDIDPNELSESDLVLDWRADDQCETQNLRYVHQFTPEKL
                     KSLGETAGLILKETFYSDGKEGNLGLYQIWQKP"
     gene            complement(2729..3541)
                     /locus_tag="PK791_03495"
     CDS             complement(2729..3541)
                     /locus_tag="PK791_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOH92411.1"
                     /translation="MLAFFEKFSPVVQALFGTLFTWGMTALGAAGVFLQKEINQKLLD
                     GMLGFASGVMIAASYFSLLAPAVEMSVNLNVPQWFPAVAGFLLGGVFLWIVDKILPHL
                     HPGDKKPEGVKSSWQRSVLLVVAITLHNIPEGLAVGVAFGAAAAGIEGASLAGAIALA
                     LGIGIQNFPEGMAVSMPLRKEKLSRGKAFFLGQLSGVVEPISAMIGAALVLAMRSLLP
                     YALAFAAGAMIYVVAEELIPEAKRESNRHSDIPTIGVMLGFAVMMLLDLALG"
     gene            3586..>4583
                     /locus_tag="PK791_03500"
     CDS             3586..>4583
                     /locus_tag="PK791_03500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92412.1"
                     /translation="MASFYQNQAPNQNIADKLTLFETCLNARLNDESLKDTQAFRLFN
                     GFYEGIPGLIVDRFAQTLLIKNFARQPQTLEGLIQPTIDFYKKNIPEINAVVLKTRHA
                     KQENQRVGQILEGQPDQEILENGIRYSLNLQLNQDDSFYLDTRHLRHWLSQNMSDKTL
                     LNTFAYTGALGIAALSGGATSVIQTDLNAKYLQVAHDSTQRQNNAGKHQILAGDFFKQ
                     MARFKSQNLLFDAVIIDPPLYSKTQAGEVSLLNNWEKLVNKVRPLVAHDGWLVLINNA
                     LYLPGKTLYAQIQKMCASGYMEIEEIIDIPQDITAYPHTIKTPVQVDPKPYNHPTK"
BASE COUNT         1271 a         1254 c          920 g         1138 t
ORIGIN      
        1 cattgagact gtaaaaggct tcaaaaatgt cctcttcggt ggcgaaggtc tcttcttaag
       61 cgttgttgaa ggccccggcc gtgtttggct gcaaaccgtc agcgcctcag aatttgccca
      121 gcgcttgatg ccattccttc ccaataaagg ctaggatata acgtaagttc ataaaaaaag
      181 ccttctcaca aaagaaggct ttttcgtttt tgaaaattag tccttaaact ggcttaacca
      241 tatataactc acccagttca aaaccacgac cggcataata agcccgggta cctaccgccg
      301 caataaccgc aatcttttga tagccatggt ctttggcaat ccgttccgcg tgcgcgataa
      361 gtgacttgcc aataccagcg tgctgcgcca taccttcctg ttcggtaccg acttccaaag
      421 actgtccata aacgtgcact tcacgaataa tggcggcatc ttttaaatca tctatatcca
      481 aagcgttatc atcattaggc aatgaaagcc gcaaaaaacc tgccagttta tcatcagcag
      541 tgttatagga cagaaaatgt tcctccgcgc cagcagcctg ataactgaag tcatcataaa
      601 ccaactcgcc agcttccatt ctttgttccc gaatttctct gcatcgcacg cactcacatt
      661 ttgttccgcg ccgtgccatt tccacggcaa cgtcctgacg taaactggta ttgcgcgacc
      721 cttcaatcac attcgtgctg ggaatatcgc gaataacgcg gttaatgcga cagtaccttg
      781 gaaccatcgt cttgatatcc gccaaaagat ttaacaaggt ggtttcatcg taaggttcgt
      841 aatcgccatc ctgccaaatc ccataaagtg gcgcgctgtt cagcaactga catggataga
      901 ttttcagttc atcgggattg acgccacttt cttcccacaa gcgcttgaaa tcttctttat
      961 caaggtctgg tgtagaccct aacaagttgg gaatccagtg ggcggtaatt ttaaagcctg
     1021 cttcgcgtaa taaagccgtt gcttttaagg ttgtcgcgct gtcatgcccg cgtttgtttt
     1081 tctctagaat ttcatcgttc atgctttgaa tgccgatttg aatttttgtc acgccttgtt
     1141 tacgtaaaag cttcaattct tcggcattga ttaaatctgg acgggtctca atcactaatc
     1201 cgacgttccg tttttcagcg ttcacatttc gcgcctgaac ttcatatagt ggaacatcac
     1261 cctggtcgtc caacgggttt tcaccgttca tcgcttcaaa acaacgtctt aaaaaccatt
     1321 cccggtaatc tttcggataa gccgaccaac tcccccccaa tatcagcagt tcaatcttct
     1381 ccgtgggatg accaacgtca tgcaaggctt tcaggcgcga agcaacctga cgataagggt
     1441 caaattcatt ttccaaaccc cgtctggctc cgggttcatc cggtaaatag ctttgtggca
     1501 ttccttcaat ggttggacaa aacacacact ccccagggca aggatgcggc ttggtcagca
     1561 ccgtcaacgt ggtaacaccc gaaagcgtgc gaatcggctt catgcgaatt tttgccagca
     1621 ataccgggtc ttcttcccaa atttcctgtt caaccaaacg tcgataagct gccaccagag
     1681 cgtgcttagc gagaaaaccg gcaccacccg gcaaaggata catccgctgg gcgttgcgca
     1741 cctcttgccc atcacgcacc gcttcaagta cttttcgcgc aaggacaagt tcttcttcgg
     1801 tataagttcg ttttttacgc catgctttgc gtatttgttc atctaacata atcacattgt
     1861 aaccgtttat ccaatgaatt tctacacagc gaatgtcaga ttgaaaaaga aatcgccttc
     1921 cgttatgcgc cagtcggtca ggtgaagtac aatcatgtca tgccgaaaat gagaccagaa
     1981 accagccaaa aacttctcgc gttgaataaa agcttttacg agaacaacgc ccagagtttt
     2041 tcttcaaccc gctacgccat ccaacccggt gtgcgcagtc ttttgccgag tatgctaacg
     2101 gccaggacga ttctagactt aggttgtgga aatggtaatc tagcccttgc cttggataaa
     2161 gccggttttt gccatgaata ttttggtgtg gacaacagcc agtgcttgat tgatgacgcc
     2221 atttccgcca gagataaacg catcgccaaa cgtgcccatt acacatttca agttcttgat
     2281 ttaggccaaa cgccctggca aatagctgca gaccgacgtc agttcgacat gattgtttct
     2341 tttgccaccc tgcaccatat ccctggtctc cgcctgcaac aagaattctt tatgcaagct
     2401 gccagcttac tggagaccaa tggcacattt attattagct gctggcaacc cctcaacagt
     2461 ccccgcctgg caaagcgcgt tcaaaactgg gatgtgcttg atatcgaccc aaacgaactt
     2521 tcagaaagcg acttagtcct cgactggcgc gcggatgacc aatgtgagac tcaaaacttg
     2581 cgctatgtgc atcaattcac gcccgagaaa ctaaaaagcc tgggtgaaac cgcaggctta
     2641 atcttaaaag aaacgttcta cagtgatggc aaagaaggca atttgggcct ttaccaaatc
     2701 tggcaaaaac cctagccacc attaaacttt agcctaaagc caaatccaaa agcatcatca
     2761 ccgcgaaacc aagcatcact ccgatcgttg gaatatcgct gtgtcggttc gattcgcgtt
     2821 tggcttcagg aataagttct tccgcgacga cataaatcat cgcgcccgct gcaaacgcca
     2881 gcgcgtaagg cagcaaactt cgcatcgcaa gcaccagagc tgcgccaatc attgctgaaa
     2941 tcggttcaac aaccccactg agttgaccca ggaaaaacgc ctttccacgg cttagctttt
     3001 ccttgcgcaa tggcatcgaa accgccatgc cttccggaaa attctgtatg ccaataccca
     3061 aagccaaagc aatcgcgcca gctaacgaag caccttcaat acctgccgcc gcggcaccaa
     3121 acgccacacc gactgccaga ccttcgggga tattgtgcaa cgtaatcgcc acaaccagta
     3181 aaacactgcg ttgccagctg cttttcacac cttccggttt tttatcgcca ggatgcaaat
     3241 ggggcaggat tttatccaca atccacagga aaacaccacc taacaaaaag ccagccaccg
     3301 cggggaacca ttgtggcaca ttcaaattaa ccgacatttc caccgccggg gcaagcaagg
     3361 aaaaatagct agcggcaatc atcaccccag atgcaaaacc aagcatgccg tccaggagtt
     3421 tttgattgat ttctttctgc aggaaaacgc ccgctgcgcc taatgcggtc atgccccagg
     3481 tgaacagggt gccaaacaag gcttgcacaa ccggactaaa tttttcaaaa aaagctaaca
     3541 tatcgctctc cagtgataag attatgacat aaggatctta tgtttatggc gtcattctat
     3601 caaaaccagg caccaaatca aaatatcgcg gataaactta cgctttttga aacttgctta
     3661 aacgcgcgcc taaacgatga gtccttgaaa gacacccaag cctttcgcct ctttaatggc
     3721 ttttatgaag gcatccctgg cttgatagta gaccgcttcg ctcaaacttt gcttattaaa
     3781 aacttcgccc gacagccaca aacgcttgaa ggtctcattc agcccactat tgacttttat
     3841 aaaaagaata ttcctgagat taacgcggtt gtgctcaaaa cccgtcacgc aaagcaagaa
     3901 aaccaacgtg ttggacaaat ccttgaaggc caacccgacc aggaaatcct cgaaaacggt
     3961 atccgttaca gtctcaatct gcaactcaac caggatgaca gcttttatct cgacacgcgc
     4021 catctccgcc actggctaag ccaaaacatg agcgataaaa cgcttttgaa cacctttgcc
     4081 tacaccggtg ctttaggcat cgctgcactg tcaggtggcg caacgagcgt tattcaaacc
     4141 gatttaaacg caaaatattt gcaagtcgcc catgattcaa cccaacgcca gaataatgcc
     4201 ggcaaacatc agattcttgc gggagatttc tttaaacaaa tggcgcgttt caaaagccaa
     4261 aacctgcttt tcgatgccgt catcatcgat ccacccctct attcaaaaac ccaggctggt
     4321 gaagtcagtt tgcttaataa ctgggaaaag ctggtcaaca aagttcgccc acttgtggca
     4381 catgacggct ggctagttct catcaacaac gcgctttact taccaggcaa aaccctttac
     4441 gcgcaaatcc aaaaaatgtg tgcttctggc tatatggaaa ttgaggaaat cattgatatt
     4501 ccccaggata tcaccgctta cccccacacc ataaagaccc cggtccaggt tgacccaaaa
     4561 ccttacaacc accctaccaa aat
//
LOCUS       DAOSFC010000098         4521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_17226_length_4521_cov_5.522839, whole genome shotgun sequence.
ACCESSION   DAOSFC010000098 DAOSFC010000000
VERSION     DAOSFC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17226_length_4521_cov_5.522839"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..770)
                     /gene="infC"
                     /locus_tag="PK791_03505"
     CDS             complement(252..770)
                     /gene="infC"
                     /locus_tag="PK791_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOH92413.1"
                     /translation="MSSKQEHRVNERIRSERVRLVDENGENAGIVDLDYALHRAEDVG
                     LDLVEIAPNANPSVVKIINYSKFLYDKEKKEREARKAQTKVEIKEIRLRPKTGDHHHD
                     IKVKDARRWLNNGMKVRVTVRFRGREITYPELALEDLREIAEELADVADIEQAPNMEG
                     RSMFMMLAPKKN"
     gene            complement(918..1652)
                     /locus_tag="PK791_03510"
     CDS             complement(918..1652)
                     /locus_tag="PK791_03510"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HOH92414.1"
                     /translation="MNNPNLQTENAPNKRFVLPAEISLLLGLVLLTFSIGFIRASNFG
                     VAVLTSVPFVISQKYTFFSAGTWSTIIMLLLLLVLIICTKKKLTAALMSLILSFAFGG
                     LLDLAEMALAGLPTGLPLRILYYIIGFFMIPLCITLFEKSGYPMLPFDSFVRDMSNYY
                     GWKFSVAKTIFDVGFVGFSVLFSIFALGGLRDVGIGTVISAVFTGYFIKLFSDLLDKV
                     MVVEPWFPKVRAFFSDTPREKESSQA"
     gene            complement(1655..2059)
                     /locus_tag="PK791_03515"
     CDS             complement(1655..2059)
                     /locus_tag="PK791_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92415.1"
                     /translation="MEKIKNLLSKINWTDKRLIAIVLVVLLILLMMGFNNRLVNMNRL
                     IRQENILQTRIAGLESTKIAVEEQVAYATSEIALEEWARESNHMIEPGDQAIVLIAPD
                     EQLSTPVPTPVAEEEIKLSNFETWKLLLFGED"
     gene            complement(2178..2262)
                     /locus_tag="PK791_03520"
     tRNA            complement(2178..2262)
                     /locus_tag="PK791_03520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2226..2228),aa:Leu,seq:cag)
     gene            2495..4060
                     /locus_tag="PK791_03525"
     CDS             2495..4060
                     /locus_tag="PK791_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOH92416.1"
                     /translation="MNLSKSLRKIIIALLIPGFLLSACVQVSRPFEPWKAATPQSRLA
                     QVETPETEPTPDSEATAVPQITLTSAPQQAMPTPNTAFLDAEERTENIIYTVKEGDYL
                     GAIATKHRVSVNQIIRANAGVNLGVIHPDQKLVIPPDSQNPFASDFKIIPDSELVYGE
                     SLKDFDTIDVVNQFNGALSRFTETFWDGRTYTGAEVVQMVADENAVNPRLLLAILEYH
                     GGWLTQERVSEETRLYPIGYKDYKLDGMWEYLDWAADRLMGGYTAWPKAYLSTWSLPD
                     EKVYRIAPTINAGTAGVQNLYARYLYDFQFVEAVSETGLYATYQKLFGDPFAFTANAS
                     LPEGLTQPEFILPFSYGETWSFTSGPHYGWGVGSPWAALDFAPPGGEQDLGCYTSYSP
                     VLAVADGVIARSDVGFVVLDLDGDGSEQTGWTVHYIHIGSEGRIKAGTHVATGDVIGV
                     ASCEGGYSTGTHFHIARRYNGLWVDAYGAVPFNLDGWIAYSTGIAYNGYLEKNGKRIE
                     AYYGRSPVNEIRR"
     gene            4044..>4521
                     /locus_tag="PK791_03530"
     CDS             4044..>4521
                     /locus_tag="PK791_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92417.1"
                     /translation="MKSGDSQKQEKRQQKRLTRRRILLVWSAVTFLVLGTFIIQAGRN
                     RAPQAAVLKDLPAANLLGSYSLGSVENDIDSPEPVPEQDALAMAGNEELQPTTEKESE
                     PDAPAETQLPPSEETPNEPETSVLPTNAELNTYSETAPVTYYAQSGDSLGVLAVRFG"
BASE COUNT         1169 a         1042 c         1136 g         1174 t
ORIGIN      
        1 cgattaaact cctctaagaa aatttcaacg gctgataacg tactcagccg tcgcttaaat
       61 accaaactga gattatacca gaaattctgt aaagtcaata ggcttgaact gataaattga
      121 cgaatctcat catgataagc agctttttat gacaccagct tgaaaaactc aaatcagtac
      181 agcttaatca agcccgctca tggcaccatg agcggagcgt ctttgcagta ctgaattgtt
      241 gattgataaa cttagttttt cttgggtgcc aacatcatga acattgaacg cccttccatg
      301 ttgggggctt gttcaatatc tgccacgtcg gcaagttctt cggcaatttc gcgcaagtct
      361 tccagtgcca gttccggata ggtgatttcg cgacccctaa agcgcacggt tacgcgtacc
      421 ttcatgccat tattcagcca gcgacgcgcg tctttcacct tgatgtcatg atggtgatcg
      481 ccagttttgg gacgcaggcg aatctctttg atttccactt tggtttgggc tttgcgggct
      541 tcgcgttcct tcttttcttt gtcgtaaagg aacttgctgt agttgatgat tttgacgaca
      601 gacgggttgg cgttgggggc gatttcgact aagtccagtc caacatcttc ggcgcgatgc
      661 agggcatagt caagatctac aattccggcg ttttctccat tttcatccac caggcgaact
      721 cgttcgctac ggatgcgttc atttacacga tgttcttgtt tactgctaat ctctttttct
      781 cctttttatt aaaataaata gcctttggct gacattaata ttaccatagg ctaagaatat
      841 gcgtcaatga aaataaggca attaacgata ttgttttttt gtatgttgtt tttcttacta
      901 taccgtcacg gtgtcgttta ggcttggctg ctttcttttt cgcgtggcgt gtcgctgaaa
      961 aaggcacgca ctttggggaa ccagggttcg acgaccatca ctttatccag cagatctgaa
     1021 aaaagtttaa tgaaatatcc ggtgaacaca gcactgataa ccgtaccgat gccaacgtcg
     1081 cgcaggccac ccagcgcgaa gattgaaaac agaactgaaa agccaacgaa gccgacgtca
     1141 aaaatggttt tggcgacgct aaatttccag ccataatagt tgctcatgtc gcgcacaaag
     1201 ctgtcaaagg gcaacatggg atagccggat ttttcaaaaa gggtgataca caacggaatc
     1261 ataaaaaagc cgatgatgta gtacaggatt cgcaagggca ggccggtagg caagccggca
     1321 agggccattt cggcaaggtc taacagtcca ccaaaggcga aagaaagtat cagggacatt
     1381 aaagccgcgg tgagcttttt cttggtgcaa atgatgagca cgagcagtaa aagaagcatg
     1441 ataattgttg accaggtgcc agcgctgaaa aaggtgtact tttgcgagat gacaaagggc
     1501 accgacgtga gcactgccac gccaaagtta ctggcgcgga tgaagccgat gctaaatgtg
     1561 agtagcacta aacccagcag gagcgagatt tctgcgggca gtacaaagcg cttgtttgga
     1621 gcgttttcag tttgtaaatt ggggttattc aaaattaatc ctcaccaaag agtagtagtt
     1681 tccaggtttc aaaattgctc agcttgattt cttcttccgc gaccggtgtg gggaccgggg
     1741 tgctgagctg ttcgtctggg gcgataagca cgatggcctg gtcgccgggt tcaatcatat
     1801 ggttgctctc gcgtgcccat tcttccagcg cgatttcgga cgtggcatag gcaacttgtt
     1861 cttccaccgc gattttggtg ctttcgagcc cggcaatgcg ggtttgcagg atattttcct
     1921 ggcggatgag gcggttcatg ttgacaagac ggttattaaa acccatcatc aggaggataa
     1981 gcaataccac aagcacaatc gcgatgagac gtttgtcggt ccagttgatt ttcgagagca
     2041 ggtttttaat cttttccatc gttttaatat ctgcaattat tgtaccatct aagtttcttt
     2101 gtggaacagc ttatagtggc gttttgcttt tttaaaaaca aaaagttaca gcctgaaagc
     2161 tataactgat ttgctgatgc cgaaggaggg acttgaaccc tcacgagcac tgtgcccact
     2221 acgccctgaa cgtagcgcgt ctgccaattc cgccacttcg gcctggacgt ttattttagc
     2281 gcaaatccta aaatagtcaa tgttttttga gtgcgcaaat caaaaattct aatctcattg
     2341 tgaatgtggt ctcctgaccc catgctaaca agggaaaacc acgttagaaa agggggcgaa
     2401 caagggaaaa actcgccacc ataaaaatac ggcttcagaa tgcgctggca cggaagatgc
     2461 aaagctattt caacagtaat ttgagaacag gcttatgaat ctttcaaaat ctttgcgaaa
     2521 aatcattatc gcgcttttaa tccccggttt tttgttgagc gcttgtgttc aggtgtcgcg
     2581 accgtttgaa ccctggaagg cagccacgcc acaatcacgt ttggcacagg ttgaaactcc
     2641 agaaacggaa cccacgccag actctgaagc tacggcggtg ccccaaatta ccctcacaag
     2701 cgcgccccag caagccatgc caacgcccaa tacggctttt cttgacgcgg aagaacgtac
     2761 tgagaatatc atctatacgg tcaaagaggg tgattacctg ggcgcgattg ccacaaaaca
     2821 tcgcgtttcg gttaatcaga ttatccgcgc gaatgcgggt gtcaatttgg gcgttatcca
     2881 tcctgatcaa aaattagtca tcccgcctga cagccaaaac ccattcgcgt ctgatttcaa
     2941 aatcatccca gattcagagt tggtctacgg cgaatccttg aaagatttcg acacgattga
     3001 cgtcgtcaat caattcaacg gcgctttgag ccgttttacc gagacctttt gggatggtcg
     3061 cacctatacc ggcgcggaag tggtgcagat ggtcgctgat gaaaacgcgg tgaatccgcg
     3121 cttgctgctg gcgattttgg agtatcacgg gggctggctg acccaggaaa gggtgagcga
     3181 agaaaccagg ctttatccga tagggtataa agattacaaa ctggacggca tgtgggagta
     3241 tctcgactgg gcggctgacc gtctgatggg tggctacact gcctggccaa aagcttattt
     3301 gagcacctgg agtttgccgg acgaaaaggt ctaccgcatc gcgccaacga ttaacgctgg
     3361 tacggctggt gttcagaacc tctacgcgcg ctatttgtac gattttcaat ttgttgaagc
     3421 ggtatcggaa acgggacttt atgccaccta tcagaaactg tttggtgacc ctttcgcgtt
     3481 taccgcgaac gcaagtttgc ctgaagggct aacacagcct gaatttattt tgcccttttc
     3541 gtatggggag acatggtcgt ttaccagtgg tccgcattac ggctggggtg tgggcagtcc
     3601 ctgggcggca ctggattttg cgccacccgg tggtgaacag gatttgggct gctacaccag
     3661 ttattcgcca gttttggctg ttgcggatgg ggtgattgcc cgttccgacg tcggttttgt
     3721 ggttttggac ttggatggcg atggctctga acaaaccggc tggacggtgc attatatcca
     3781 tatcggctca gaaggaagaa taaaggctgg cacacatgtt gcaacaggag atgtaatcgg
     3841 tgttgcatcc tgcgaaggcg gttacagcac cggcacgcac tttcacattg ccagacgcta
     3901 taacggactt tgggtcgacg cttatggcgc ggtgcctttc aatttagatg gttggattgc
     3961 atatagcact ggcattgctt acaacggata cctggaaaaa aatggaaaga gaattgaagc
     4021 ttactatggc cgttcccctg tcaatgaaat caggagatag ccaaaaacaa gaaaaaagac
     4081 aacaaaaacg cttaacccgg cgacgcattt tgctggtatg gtctgcagtt acctttctgg
     4141 ttttaggtac gtttattatc caggcggggc gtaaccgtgc accccaggcg gctgtactga
     4201 aagacctgcc cgctgcgaat ttgctgggaa gctattctct tggatccgtt gaaaatgata
     4261 tcgacagccc cgagccagtt cctgaacaag atgctttggc tatggctgga aatgaagaac
     4321 tgcaaccgac aaccgaaaaa gaaagtgagc ctgatgcacc cgctgaaacg cagttaccac
     4381 caagtgaaga aacaccaaac gagccggaaa catctgtttt gcctacaaac gctgagctga
     4441 atacatactc agaaaccgcg ccggtaacct attacgccca aagtggcgac agcctgggtg
     4501 ttttggcagt gcgctttggt g
//
LOCUS       DAOSFC010000099         4517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_17255_length_4517_cov_4.097714, whole genome shotgun sequence.
ACCESSION   DAOSFC010000099 DAOSFC010000000
VERSION     DAOSFC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17255_length_4517_cov_4.097714"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PK791_03535"
     CDS             complement(<1..888)
                     /locus_tag="PK791_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92418.1"
                     /translation="MTRYPKLSPRAFDELSAYIDGQLKPQEALAFEKKLSRSSKLQEA
                     HREVLAMRKATRALPQRKAPRNFILTRAEAAEARRGRNLSRVFGLAFSLCAVFLVVLF
                     GYDGLSKGLFAMKAAAPEMAMPEEMPQAYALRSAPDLAAELPVEMLDESVEEPVEEQA
                     TEEAVLLTWGAPSAMTAEGSGAMDSQDLPVPMQSPYQSDSALASKPTQVLTTEMDFIK
                     IHLNPVTGAVTICDPVYGCGYGGSIQTQSGTHPEAKNEIYVDPALIYFDQRTGLLKLC
                     PELGACPTGDENLIAVKAVE"
     gene            complement(881..1492)
                     /locus_tag="PK791_03540"
     CDS             complement(881..1492)
                     /locus_tag="PK791_03540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOH92419.1"
                     /translation="MDENTLIDLAKKGELDAFNQLVLSYQDMAFNFAYRIMNDDAAAA
                     DATQEAVISMYRRIDTFRGGSFKAWFLRIVSNQCYDNLRWQKRRPSVALEPETDDGER
                     FDSPAWLEDDAPKPEDQLERTELEAAIQHCINALPPNFKTVFLHVDVNGEDYETVAEI
                     IQSPVGTVKSRLARARQRMQGCLQGFRELLPEIFRLNSEDEDD"
     gene            complement(1528..3054)
                     /gene="lysS"
                     /locus_tag="PK791_03545"
     CDS             complement(1528..3054)
                     /gene="lysS"
                     /locus_tag="PK791_03545"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOH92420.1"
                     /translation="MLLDDFSELENTRLEKIHRMRDEGIEPYPTRSAKSMNNADAVKA
                     FETAEAEGKAEVPDVVLAGRIRAIRNMGKLCFTHIEDSSGKIQLFLRINELGEEKLNS
                     FVRDFDLGDFIEASGALTRTRRGEITLMVHDFKMLAKALYQLPADKDQVVDGETVRHA
                     VLSDPETRYRQRYVDLAVNPDVREIFKARAKIVDTVRTYLDERDYIEVETPILQPIYG
                     GAAARPFTTYHNQLHQELFLRISFELYLKRLIVGGMDRVYEIGRDFRNEGVSFKHNPE
                     FTQLELYEAYTDYHGMMRIAEEMVSQCAYELNGHYIINYDGNEIDLTPPWKRITIRQG
                     LIDFADIDIKDYPTVESLEALLKERDIPFKPGVPHGKLIGLLMEEYIEPRLIQPTFLC
                     DYPRDISPLAKNVPGDPSTVERFEIFIGGVELGNAFTELNDPVEQEKRFLEMADAYAK
                     DDEEQHPMDHDFVRALAYGMPPTGGMGIGIDRLVMMLTGKNTLREVILFPHLRKIEDQ
                     "
     gene            complement(3134..3601)
                     /gene="greA"
                     /locus_tag="PK791_03550"
     CDS             complement(3134..3601)
                     /gene="greA"
                     /locus_tag="PK791_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOH92421.1"
                     /translation="MPSSYLTRQGYEKSEQELEFLRTEKRKQVAARLQDAMEDGELIE
                     NAEYEDAKNEQAFVEGRIKELEILLSNAYIIDDNDNPEGTVAVGSTVTILIEGDDEPV
                     TYMIVGAAEANPRENRISNVSPIGHALLGKQVGDKVKVESPGGAFYVEILGSK"
     gene            complement(3855..4205)
                     /gene="rplS"
                     /locus_tag="PK791_03555"
     CDS             complement(3855..4205)
                     /gene="rplS"
                     /locus_tag="PK791_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOH92422.1"
                     /translation="MNHTELLKSVEAEKNPNIPTLFPGDNVSVHMKIKEGDRERIQEF
                     KGVVLYTRGRGNDASFTVRRVASNGIGVERTFLYHSPRIAKVVVERHNKVRRARLYFL
                     RERTGKSARLKQRF"
BASE COUNT         1136 a         1108 c         1083 g         1190 t
ORIGIN      
        1 ctcaacagcc ttaacagcta tcaggttttc atcacccgtc gggcacgcgc cgagttcagg
       61 gcaaagtttc agcaaacccg ttcgctggtc aaaataaata agcgcggggt cgacatagat
      121 ctcattttta gcttccggat gagtcccact ttgggtctga atgctgcccc cataaccaca
      181 accgtagact gggtcacaaa tcgttaccgc gccagtaaca ggattgagat gtatcttgat
      241 gaaatccatt tcagttgtta agacttgggt aggcttggac gccaacgcgg aatccgactg
      301 gtacgggctc tgcatgggaa caggcaggtc ctgagagtcc atcgcgccac taccctcagc
      361 agtcatcgcg cttggcgcgc cccaagtcag caaaaccgcc tcttctgtgg cttgttcttc
      421 cactggctct tcaacagatt catcaagcat ttctaccggc aactctgcag caaggtcggg
      481 cgctgaacgc aaagcataag cctgtggcat ttcctctggc atagccatct ctggcgcggc
      541 tgctttcatg gcaaacaagc cctttgacaa gccatcatag ccaaacagca cgaccaaaaa
      601 gacagcacac aggctaaaag ccaaaccaaa aacgcgactc aaattccgtc cgcgcctggc
      661 ttcagctgct tcggcacggg taaggataaa gttgcgcggg gctttgcgtt ggggcaaggc
      721 gcgggtcgcc ttgcgcatgg caaggacttc gcgatgggct tcttgcaatt tgctggaacg
      781 ggaaagcttt ttttcaaacg ccagagcttc ctggggtttc aactgtccat caatataagc
      841 gctgagttca tcaaacgccc gcggagataa tttcgggtat ctagtcatct tcatcttcgc
      901 tatttagacg aaatatctcg ggtaaaagtt cccgaaatcc ttgcaaacaa ccttgcattc
      961 tttgtcgcgc gcgcgccagg cgacttttta cggtgccgac aggactttga atgatttcag
     1021 ccacagtttc atagtcttca ccgtttacat ccacatgtaa aaagaccgtc ttaaagttgg
     1081 gcggaagggc attaatgcag tgttgaatag ccgcctcaag ctcagttcgc tcgagctggt
     1141 cttcgggctt gggagcatca tcttcgagcc aggcgggaga gtcaaagcgt tcgccgtcat
     1201 cggtttcggg ctccaaagcg acgctgggac ggcgtttttg ccagcgcaga ttgtcgtaac
     1261 actgattgct gacgatgcgc aaaaaccagg ccttaaaaga cccgccccga aaggtatcaa
     1321 tgcgacgata catcgagatg accgcttcct gggtcgcgtc agcagccgcg gcatcatcat
     1381 tcataatgcg ataagcaaag ttgaacgcca tatcctgata actcaggaca agctgattga
     1441 atgcgtcaag ctcgcctttt ttggcaagat caatgagtgt gttttcgtcc acaacttaat
     1501 tgttgcaaga cttaagccag tcagtttcta ctggtcctca atcttgcgta agtgcgggaa
     1561 gagaataacc tcccgcaggg tgtttttgcc ggtgagcatc atcaccagcc ggtcaatgcc
     1621 gatgcccatt ccgccggtgg gtggcatgcc ataggcaagc gcgcgcacga aatcatggtc
     1681 catcggatgt tgctcttcgt catccttggc ataggcatct gccatttcca aaaagcgttt
     1741 ttcttgttca accgggtcgt tgagttcagt aaaggcattg ccaagttcaa cgccaccgat
     1801 gaaaatctca aagcgttcaa cggtgctggg gtcgcccggc acgttcttag ccagtggcga
     1861 gatgtcgcgc gggtaatcac acaagaaggt gggctggata aggcgtggtt cgatgtactc
     1921 ttccattagc aaaccgatca gcttgccgtg tggcacaccg ggtttgaagg gaatgtcgcg
     1981 ctctttcagc aaagcttcca gtgactcaac ggtggggtaa tctttaatgt cgatatccgc
     2041 gaagtcaatc agtccctggc gaatggtgat gcgtttccag ggtggcgtaa ggtcgatttc
     2101 gttaccgtcg tagttgataa tgtaatgacc attcagttca taagcgcact gcgaaaccat
     2161 ttcttcagcg atgcgcatca tgccgtgata gtcggtgtaa gcctcataaa gctcaagctg
     2221 ggtgaattcg gggttgtgct tgaaagagac accctcgttg cgaaaatcgc gtccgatttc
     2281 gtaaacacgg tccatgccgc cgacgatgag acgtttgaga tacagctcaa agctgatgcg
     2341 taaaaagagt tcctgatgca gctggttatg gtaagtggta aaaggtctgg cagcggcacc
     2401 cccatagatg ggttgcaaaa tcggcgtttc tacttcaata taatcgcgct cgtccagata
     2461 ggtgcgcacg gtatccacga ttttagcgcg tgctttgaag atttcgcgca catccggatt
     2521 gaccgccagg tcaacatagc gttggcgata gcgggtttca gggtcgctaa gcacagcatg
     2581 gcgtaccgtt tcgccatcca caacctggtc tttgtcagcc ggaagctgat aaagcgcctt
     2641 ggccagcatc ttaaagtcgt gcaccatgag tgtaatctca ccacggcgcg tgcgtgtaag
     2701 cgcgccggaa gcctcaataa aatcgccaag gtcaaaatca cgaacgaagc tgttgagttt
     2761 ttcttcgccg agttcgttga tacgcaagaa aagttgaatt tttccgctgc tgtcctcaat
     2821 atgcgtgaaa caaagtttac ccatattacg aatggcacga atacgcccgg ccaggacaac
     2881 atcaggcact tcggcctttc cttcagcttc ggcagtttca aatgccttta cagcatcagc
     2941 attgttcatc gatttagcag atcgggttgg gtaaggttcg atgccttcat cgcgcatgcg
     3001 atggattttt tcaaggcggg tattttctaa ttcagaaaag tcgtcaagca gcatttatta
     3061 atcctctttt caaagtatct gtatcgtata aaatagcccc acctgttttt gggtggggcg
     3121 ttatgttttt gtcttattta cttcctaaaa tctcaacgta aaacgcacca cctggtgatt
     3181 ccactttaac tttatcgcca acctgtttac caagcagcgc atggccgata ggtgaaacat
     3241 tagagatgcg attttcgcgc gggtttgctt cagccgcacc gacaatcata taggttacgg
     3301 gttcatcatc accttctatt aagattgtca cagtggaacc aacggcaaca gtgccctcgg
     3361 gattgtcatt gtcgtcgata atgtaagcgt tcgaaagcag aatttcaagc tctttgatgc
     3421 gtccttcaac aaaagcttgc tcgtttttag cgtcttcgta ttccgcattt tcaataagct
     3481 caccatcttc catggcatct tgcaaacggg cagcgacctg tttgcgcttt tcggtacgta
     3541 gaaactccag ttcctgttca gatttttcgt agccctgacg ggtaaggtat gaacttggca
     3601 tagactaaat cctcatgctt atttggttaa tactgagtat taacataaaa cgcgcttaga
     3661 tagcttaacg ctataaaacg ccggtcatta attatactat gaatagggtg tttgggttta
     3721 atcaatatac tattgcgtct ttaattaagt tttcgttaat actgagtgga aaaaccctct
     3781 caatgtagca aaaagccccg ctctggaaca aattttgacc agaacaggct ttttgctgtt
     3841 gatataaaac aaatttagaa gcgttgttta agtcgggcac tcttgccagt gcgttcgcgc
     3901 aggaaataca agcgcgcgcg acggactttg ttgtgtcgct ccaccaccac cttggcaatg
     3961 cggggtgaat ggtacaaaaa ggtacgttca acgccgatac cgttgctggc aacgcgtcgt
     4021 acggtgaagg aggcatcatt gccacgtccg cgggtataga gcacaacgcc cttaaattcc
     4081 tggatacgtt cgcggtcgcc ttctttaatt ttcatgtgaa cactcacatt atcgccgggg
     4141 aaaagagtcg gaatgttcgg attcttttcg gcttcgacgg attttaataa ttctgtatgg
     4201 ttcatcgtac ttccctttac ttactcacat aataaacaaa tgccttccca ggcacgaact
     4261 cgcattataa caccacctat tttaaaaatc aagcgatttg aaacggataa taaattctca
     4321 tccacattct gaaaacattt ttataagtat gaaaaagaac aaaacaaaat agactgttgc
     4381 catgcttttg gggcagtctc taaaacctgt gtacggctat gagatgcaag tcttgcaaat
     4441 aaagttcgta tgttcgtttt tgagtggacc cagagagatt cgaactctcg accttctcaa
     4501 tgccattgag acgcgct
//
LOCUS       DAOSFC010000100         4489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_17442_length_4489_cov_2.705909, whole genome shotgun sequence.
ACCESSION   DAOSFC010000100 DAOSFC010000000
VERSION     DAOSFC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17442_length_4489_cov_2.705909"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PK791_03560"
     CDS             <1..294
                     /locus_tag="PK791_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92423.1"
                     /translation="GPNELDESFEAEAIRELNEALPTRPVREQPPAAQVFEPSESFES
                     PISSKAFDAPEWPSETESSTPTAETLSQEELDALYEDDFDDNAPLQTKPIKKD"
     gene            297..1796
                     /locus_tag="PK791_03565"
     CDS             297..1796
                     /locus_tag="PK791_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92424.1"
                     /translation="MQFGKNNKTIIITIVVLTLVFLAILITIGVIISKGLIRRAETQS
                     FTQTAVVAESQASNTPQIPTPQATNTPDTASPTEDAETHAEPSPTAEATATPTQNPSV
                     SLDPETEAIMDSIEEQVREIRRLDIDHDIPRRVLSPEELNVYVNEDLLEDFDETASTA
                     SIRRFALLGLYPRDLEMRSMLEDLYSEQIAGFYRTEEREMFVVAGEKFGINEKMTYAH
                     EYIHALQFTNFDIENELGYSDEGCEEDSERCIAIQALIEGDATLSQFKWFENFATRSD
                     YIELLESMETYESPALDSAPPYLSASMMFPYEYGTNFVQYLYDQRGYQTVNNAFTSQP
                     PVTSEQIMFPERYPDDLPVDVELPDFASSMGGAWETDGIGVVGAWDIYLTLTKGIDES
                     FQLDNETAMKAVEGWGGDAYVFLVNELEDSYLFVMKTVWDTEEDSAEAWDAFVKMLRL
                     RFGWPSNEGVYSRDGYLARLFKTGDNGFVLIISEQLESLNQVYDMLTID"
     gene            1814..2548
                     /locus_tag="PK791_03570"
     CDS             1814..2548
                     /locus_tag="PK791_03570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH92425.1"
                     /translation="MPKTSKTFQSSLDRSMVKALLFDIDGTLSDSDDEMVASIYARVA
                     WLTPLFKKENLHRFVRKTVHFFQAPVNRFLAFLDHIGIDSHLARFLDYRASKKEALPE
                     DFPIIPGVRSMLEALKGHYPFAVVSARNKVTVQKFLRVNDLEDYFQIVISSQTCEKTK
                     PFADPLLHAAEELDVPIENCLMIGDTLTDVRAALAAGAQSLSVLCGFGTEKELQKSGT
                     HHILQSTSELANYLNCELSTLDKKDA"
     gene            complement(2550..4004)
                     /locus_tag="PK791_03575"
     CDS             complement(2550..4004)
                     /locus_tag="PK791_03575"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92426.1"
                     /translation="MNNDNQHELDQKNQSSNSGIPNDELIESSELITSPLDVDKEQVL
                     PLDEPEQSLNTVEQLGLPQKWLRNFLPMGIGQILSMLGSSLVQFALVWYITKETGSAI
                     SLLSATLTGTLPRVILGPFAGALVDRWNRKLTMIISDGIIALATLVLAILFATGQIQV
                     WHIFAMLFVRSVGSIFQGPAMSASISLMIPKEHYTRYAGISQAADGIITIVSPPLGAL
                     LMAYLPIQAVLAIDTLTAAIAIALLAFLVYVPQPVKEAAETLKMSVKSVFSDVRDGFK
                     YVIEWKGVFLLMIGGTLINMTASPAFSLLPLYVEQHFQKGPEGLAWLYSAFGIGSIVG
                     GLVLGVWGGFKRKILTVMMGIMGMGVGMFVLGLVPQKAYGVAVALTAFLGVMTAFANG
                     PLNAIVQEKIPNELQGRVFTVLGSLVQATVPLGLIVAAPIAELFGISIWFSVGGVVGV
                     LIAILGLVLKPLRTLDFQGPGGFILPEYQDRVKQ"
     gene            4145..>4489
                     /locus_tag="PK791_03580"
     CDS             4145..>4489
                     /locus_tag="PK791_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92427.1"
                     /translation="MTKKHFRENYQKKYPYPRYQFRRNVLRTGIAFLASILLNYKIEG
                     KENLPKEGPLLIVSNHFHFLDTIGPIHCTSYPLEFINDAVMPMAPNAMKFLPGLWKTL
                     IIQQGTANLETMR"
BASE COUNT         1358 a         1131 c          948 g         1052 t
ORIGIN      
        1 gggccaaatg aattggacga aagctttgaa gcagaagcca tccgcgagtt aaacgaagct
       61 ttgcctaccc gtcccgtgcg cgaacagccc ccggcggctc aggtgtttga accttcagaa
      121 tcatttgaaa gcccaatctc ctcaaaagca tttgacgcgc ccgaatggcc atcagaaaca
      181 gaatcatcta ccccaactgc tgaaacgctc agtcaggaag agcttgacgc gctctatgaa
      241 gatgactttg acgacaacgc accccttcaa accaaaccaa tcaagaaaga ttaataatgc
      301 aatttggaaa aaacaacaaa accatcatca tcactattgt cgttttgaca ctcgtttttt
      361 tggcgattct aattaccatt ggtgttatta tctccaaagg gctaataaga cgtgcggaaa
      421 cacaaagttt tacccaaacc gctgtggttg ctgaatcaca agcgagcaat acaccacaaa
      481 ttcccacacc ccaagctaca aacacgccag atactgcgag tccaacagaa gatgcggaaa
      541 cacatgctga acccagtcca accgctgaag cgactgccac accaacacaa aacccaagcg
      601 ttagcctgga cccagaaacc gaagcgatca tggacagcat tgaagagcag gtcagagaaa
      661 ttcgacgcct tgacatcgat catgacattc cacgccgtgt gctttcacct gaagaattaa
      721 atgtctatgt caacgaagac ctcttggaag attttgacga aacggcatcc accgcaagca
      781 tacgccgatt cgcgctatta ggattatacc cacgcgacct tgaaatgcgc tctatgctcg
      841 aagacctcta ttctgaacaa atcgcaggct tttacagaac cgaagaacgc gaaatgttcg
      901 ttgtcgctgg agagaaattc ggcatcaacg aaaagatgac ctacgcgcat gaatacattc
      961 atgccttaca attcactaat ttcgacatcg aaaacgagct tggatatagt gatgaaggct
     1021 gcgaggaaga ctcagagcgc tgtattgcca ttcaagcgct cattgaaggc gatgccacac
     1081 tttctcagtt taaatggttt gaaaacttcg ccacacgcag cgattatatt gaactcctgg
     1141 aaagcatgga aacatatgaa agtccagctt tagattccgc gccaccttat ttatccgcat
     1201 cgatgatgtt cccctatgaa tacggcacta actttgtgca atacctttac gaccaaagag
     1261 gctaccagac agtaaacaat gccttcacca gccaacctcc agtcaccagt gaacaaatca
     1321 tgttccctga acgctaccca gacgatttgc ctgttgatgt tgagttaccc gattttgctt
     1381 caagcatggg cggagcctgg gaaacagatg ggataggcgt tgtaggggcc tgggacatct
     1441 atttaaccct caccaaaggc attgatgaga gcttccaact cgacaatgaa accgccatga
     1501 aggcagttga gggctgggga ggggatgctt acgtttttct ggtcaacgaa cttgaagaca
     1561 gctatttgtt tgtcatgaaa actgtttggg acacagaaga agacagcgcg gaagcctggg
     1621 acgcttttgt aaaaatgctg cgtctgcgct ttggctggcc atccaatgag ggtgtgtaca
     1681 gcagggatgg ttatctcgcg cgccttttca aaactggtga taacggcttt gttttgatta
     1741 tttcagaaca attagaaagc ctcaatcagg tctatgatat gctgactatc gactaaagcg
     1801 gagacccatt agcatgccca aaacaagtaa aacttttcaa tcaagccttg accgcagcat
     1861 ggttaaggct ttactttttg acatcgacgg caccctctct gacagtgacg acgaaatggt
     1921 ggcttcaatt tacgcgcgcg tggcctggct tacgcccctt ttcaaaaagg aaaacctgca
     1981 tcgctttgtg cgtaaaacag tgcatttctt ccaggcaccg gttaaccgtt tcctggcttt
     2041 tttagaccac atcggcatcg acagtcacct ggcacgtttt ttagattacc gtgccagcaa
     2101 aaaagaagcc ctgcccgaag atttccctat cattccgggt gtgcgttcga tgcttgaggc
     2161 cttaaaaggc cactatcctt ttgctgttgt ctcagcgcgc aacaaagtaa ccgtccaaaa
     2221 attcctgaga gtgaatgacc ttgaggacta tttccagatt gtaatcagct cacaaacctg
     2281 cgaaaagacc aaaccttttg ctgacccact tttgcatgcc gcagaagaac ttgacgtgcc
     2341 tatcgagaac tgcctgatga ttggcgacac gctcaccgac gtacgcgcgg cattggctgc
     2401 tggcgctcaa tcgctttctg tcttgtgtgg attcggaacg gagaaagaat tgcaaaaaag
     2461 tggcacccac cacattttgc aaagtaccag cgaattagca aattatttaa attgtgagct
     2521 atccacgttg gataaaaagg acgcttagtt tactgcttca cccgatcctg atattcaggc
     2581 aaaataaagc ctccaggtcc ctggaaatca agcgtgcgca atggttttaa tacaagaccc
     2641 aaaatcgcga tcaaaacacc caccacacca cctacagaaa accatatcga aatcccaaag
     2701 agctccgcga taggggcggc aacaattaac cccaacggaa cagtagcttg aacgagcgaa
     2761 cccaaaaccg taaacacacg tccctgaagc tcattgggaa ttttttcctg cacaatagca
     2821 ttcaaagggc cattcgcgaa ggcagtcatc acgcccagga aagctgtcaa agcaaccgcg
     2881 actccataag ctttttgtgg cacaagtccg agcacgaaca taccaacccc cattcccatg
     2941 atacccatca ttaccgtcaa tatcttgcgt ttaaagccac cccatacacc taaaaccaat
     3001 ccacctacga ttgaaccaat cccaaaagcg gaatatagcc aggctaaacc ttcaggccct
     3061 ttttgaaaat gctgctcaac gtaaagaggt aaaaggctaa acgcgggtga cgcggtcatg
     3121 ttgattaagg taccgccaat catcaacaga aaaacacctt tccattcgat gacatatttg
     3181 aaaccatccc taacatcgct aaaaacagac ttaacactca ttttgagtgt ttccgcggct
     3241 tctttgaccg gttgcggaac gtaaaccaag aaggccaaaa gcgctatcgc gattgcggca
     3301 gtaagggtat caattgccaa tacagcctgg atgggcaaat aagccatcag caaagcgcct
     3361 aaaggtggcg aaacgatggt gataataccg tcagcggcct gactgatgcc agcataacgc
     3421 gtgtaatgct ctttgggtat catcaggctg atagaagcac tcatcgcggg gccctggaaa
     3481 atgcttccaa ccgaacgcac aaaaagcata gcgaaaatat gccaaacttg aatttgaccc
     3541 gtcgcaaaca gaatggccaa cacgagtgtt gccaaggcaa tgatgccatc ggaaataatc
     3601 atggtcagtt tgcgattcca acggtcaacc aacgcgccgg cgaacgggcc taaaatcact
     3661 ctgggcaaag tgccggtcag cgtggctgaa agcagtgata tcgctgaacc ggtttctttg
     3721 gtgatatacc aaaccaaggc aaactgtacc aacgaagagc ctaacatgga aagaatttga
     3781 ccaataccca ttggcaggaa attacgcagc catttttgcg gaagtcctaa ctgttcgact
     3841 gtgttcaggg actgttccgg ctcatccaat ggtaaaacct gttccttatc aacatctaac
     3901 ggtgaagtga taagttctga ggattctata agctcatcgt ttggtatgcc agaatttgaa
     3961 gactggtttt tctggtctaa ttcatgttga ttatcattgt tcataataag accttttctt
     4021 aacatgccaa attatacatt agctccacaa aagcaggaat ataagaacgc aaatagctat
     4081 ataggccctt cattttcagt aaatacgaag taaaattata acaatcaatc acgggagaag
     4141 gcttatgaca aaaaaacact ttcgcgagaa ttatcaaaag aaatacccct atccccgcta
     4201 tcaatttaga cgcaacgttt taagaacagg tatcgctttt ttggcttcga tcttgctcaa
     4261 ctacaaaatt gaaggaaaag aaaacctccc caaagaaggc ccattgctca tcgttagcaa
     4321 tcattttcac tttttggaca ctatcggccc aattcactgt accagctatc cgctggaatt
     4381 cattaacgat gccgtcatgc ctatggcacc gaacgcgatg aagttcttac ccggcctctg
     4441 gaagaccctg atcattcaac aaggtaccgc caatttagaa accatgcgc
//
LOCUS       DAOSFC010000101         4451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_17669_length_4451_cov_4.309827, whole genome shotgun sequence.
ACCESSION   DAOSFC010000101 DAOSFC010000000
VERSION     DAOSFC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17669_length_4451_cov_4.309827"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="PK791_03585"
     CDS             complement(<1..1265)
                     /locus_tag="PK791_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH92428.1"
                     /translation="MIYLSNLLNQKVWDAFGHVVGQVEDILVSHTDQAMPPIDAILLK
                     KNNSDVRLIKGELIATLWPTIALSVDQRELSDYQPHGHELHLKKRVLDQQIVDTEGKR
                     LVRVNDVVIARKNQQFLVTGVDVGIRGLLRRLGLDTAAKFLAKTFNFKLTSQIIAWED
                     VASIEHDDPLRLKVSQERLVQMEPVDIAAILDDLDHHTSKALLQGFDDEILADTLEQS
                     SFEVQQAILAAMDPERAADVLEEMDPDEAADLLAELDDEHSEQLLSLMEDEDEIEIRQ
                     LLAYPENSAGGIMTTEFAWVPGDFNVEQALTHLRTSEEAKEDEFMYYLYILDNDEKLE
                     GVVSLRDLVTAPLDKSLQDWADDDVVHVEPLTHQNETAYLVAKYDLMAIPVIDPESHK
                     MLGIVTVDDALDTVLPTAWKKRLPRFAGK"
     gene            complement(1621..2802)
                     /locus_tag="PK791_03590"
     CDS             complement(1621..2802)
                     /locus_tag="PK791_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOH92429.1"
                     /translation="MDIFKKCFDFEDARQAKAAGVYPYFLPLDRNEGTEIVYEGRRII
                     MCGSNNYLGLTAHPRVREAAIDAIKKYGTSCTGSRFLNGNMTIIEDLERDLADWVGMD
                     SALIFSTGMQVNLGTISALVGRGDIVILDKEDHASIYDGARLSHGKIERFRHNDVEHL
                     ERVLKSLPDSGGRLLVVDGLFSMEGDIAPLPEIIPLCQQYGVRLMVDDAHAMGVLGGG
                     RGTAAHFGITEGVDLIMSTFSKSFASIGGFVAGQADVIDYIKHVARSLIFSASIPPAN
                     VATAQTALNIMKEEPQLVEQVNENGRFMREGLRSLGFDIGTSESPIVPIIIGDDLLTF
                     KTWRDLFDAGVFVNPVISPATAPGRQLLRTSYMATHTQSQLEQVLEVFEKIGKQNGLI
                     S"
     gene            3073..>4451
                     /locus_tag="PK791_03595"
     CDS             3073..>4451
                     /locus_tag="PK791_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92430.1"
                     /translation="MKATAEKNRNFNKEKVINSPRLLGLWHLMKGYHFNYLVATVFLA
                     VAAFARAGIYLHLGNFIDALRSSAGLHSKAIITSALAFLGLIMLQALSTFMSSWMASF
                     AAENTTRRLRDVLFDHIQRLTYAYHAEAKTGDLLERATSDVDTLRRFFADQAIGMGRI
                     VFIFVINFALIFRMQARLAWISVVIVPVTLTISMIFFRKLRKAYEEYQEQEAIITTHL
                     QETLSGVRVVKAFARQDYETEKFAENNTEKLRRGKRMMFLHSIFWPISDIICGLQRVT
                     STYVAASAVIDQSLSLGEFVTFQGLLSWLIWPIRGLGRLIIDTSRALVSLGRILIVLK
                     AEEEDLTTAVYQPEGETKGAITFENVCFAYEENQPVLKDVSFECQPGDVIALLGSTGS
                     GKTSLVNLLPRFYDVNSGQILLDGVNLNDYSRDYLRSQIGIVEQEPFLFSCSIRDNIT
                     YGMRREVSQ"
BASE COUNT         1067 a         1064 c         1130 g         1190 t
ORIGIN      
        1 tattttcctg cgaatctggg caggcgtttc ttccacgcgg tgggcaggac ggtgtccagc
       61 gcgtcgtcaa cggtgacgat gcccaacatt ttatgacttt cggggtcaat gaccgggata
      121 gccatgagat catatttcgc caccaaataa gcggtttcgt tttggtgggt caggggttca
      181 acatgcacga cgtcatcatc agcccagtcc tgtagagact tatcaagtgg tgcggtaaca
      241 aggtcgcgca agctcaccac gccttccagc ttttcatcat tgtccaaaat gtagagatag
      301 tacataaact catcttcttt ggcttcttcg gatgtgcgca ggtgggtgag tgcttgctcg
      361 acgttaaaat cacctggaac ccaggcaaac tcggtggtca tgatgccccc ggcgctgttt
      421 tcggggtaag ccaacaattg acggatttca atctcgtctt cgtcttccat caggctgagc
      481 agttgttcgc tgtgttcgtc gtcgagttct gccaaaagat cggcggcttc gtcggggtcc
      541 atttcttcga gcacatccgc ggcgcgttcg gggtccatag cggccaaaat agcttgttgc
      601 acttcaaatg aactttgctc aagtgtgtcg gcaagaattt catcatcaaa accctgcaac
      661 aaggctttac tggtatggtg gtctaagtca tcgagaatgg cagcgatatc taccggctcc
      721 atttgcacca gacgttcctg ggagactttc aggcgtaggg ggtcgtcgtg ttcaatactg
      781 gcgacgtctt cccaggcgat aatttggctt gtgagcttga aattaaaggt tttggcaagg
      841 aacttcgcgg ctgtatctaa acccaaacga cgtaaaagtc cgcgtatgcc aacatccaca
      901 ccggtgacca aaaactgttg attcttgcgc gcgatgacca catcgttgac ccgtaccagg
      961 cgtttgcctt cggtatccac aatttgctgg tcgagcacac gctttttgag atgcagttca
     1021 tgaccatgag gctgatagtc agaaagttcg cgctggtcaa cgcttagcgc gatggttggc
     1081 caaagcgtgg cgatgagctc gcctttaatc agacgcacgt cgctattgtt tttcttgagc
     1141 aaaatagcgt caatgggggg catggcttga tcggtgtggc taacaaggat atcttccact
     1201 tgtccaacga catgcccaaa agcgtcccag actttctggt ttaacaagtt gcttaaatag
     1261 atcataagtc acctcttaat atatgtattt gtggaagaga accacggcag ttgctattcc
     1321 cctgtaaacc gaccgtggaa aggaggctta cagcggatta gcaaatactt gcagggtctg
     1381 gcgagtcgtc agagccgatg gtttcgcttc cgcttagtgt tagtgtttcc ttaaaactac
     1441 gcataactta aatagtctac cataaaaacg taaggaaatg ttgagaattt tgataggaat
     1501 cttttccgct aaacaatgag atctttagga agttgttatc tgggatcttg gtatcacaat
     1561 aatatgttta acaaaaaaca ccccaaccgg ggtgttttag aacatgtcta aaagcaaaaa
     1621 ctagctaatc aagccgtttt gcttgcctat tttctcaaag acttccaaca cttgctccag
     1681 ttgcgattgg gtgtgcgtgg ccatatacga ggtacgcaag agttgccggc caggcgcggt
     1741 ggcaggggag atgaccgggt tgacaaacac gccagcgtca aacaaatcgc gccaggtttt
     1801 gaaggtgagt aagtcatcac cgataataat cggcacgatg ggcgattcgg atgtgccgat
     1861 atcaaagccc aaacttcgca ggccttcacg cataaagcga ccgttctcgt tgacttgttc
     1921 aacgagttgc ggttcttctt tcatgatgtt cagtgctgtt tgggcggttg ccacgttggc
     1981 aggggggatg ctggcactga agattaaact gcgggcgaca tgcttgatgt agtcaatcac
     2041 atcggcttgc ccggcgacaa aaccaccgat tgaggcaaat gacttgctga acgtggacat
     2101 aatcaggtca accccttcgg tgataccaaa gtgcgccgcg gtgccacgac cgccacccaa
     2161 aacacccatg gcatgcgcgt catccaccat cagccgcaca ccatattgct ggcaaagcgg
     2221 gatgatttcc ggcaagggcg cgatgtcgcc ctccatgcta aagagcccat ccactaccaa
     2281 gaggcggcca ccgctatcgg gcaggctttt tagaacccgt tcaaggtgtt caacgtcgtt
     2341 gtgcctaaag cgctcaattt tgccatggct caggcgggcg ccgtcataga tgctggcgtg
     2401 gtcttcttta tccaggatga cgatatcacc cctgccaacc agcgcgctga ttgtgcccag
     2461 gttgacctgc atgccggtcg aaaaaatcag ggcggagtcc atgccaaccc agtctgccag
     2521 atcgcgctcg aggtcttcga tgatggtcat gttaccgttc aaaaagcgcg aaccggtgca
     2581 actggtgcca tattttttga tggcatcaat ggcggcttct cttacgcggg ggtgagcggt
     2641 gagccccaga tagttgttgg agccacacat gataatgcgg cggccttcat agacaatctc
     2701 ggtgccttcg ttgcggtcaa ggggtaaaaa atagggatag acaccagctg ctttggcctg
     2761 gcgggcgtct tcaaaatcaa aacatttctt aaaaatatcc ataatagtcc ttcattgtat
     2821 gtgctgatgc tacatatata tgataacagt ttatcacaga aatagttgct aaattttgag
     2881 ctgggaaata gcttgggatg gttttttaat gaatccgaag gattgtttta tcttgttcta
     2941 actttttttc taaaatgaat aaggcctttt aaatgtcaat agcgattgac tcttattatt
     3001 tgaggggtat actttttgcg gttattagat agacttttat ctaacactca tagatattat
     3061 ttggagttat ttgtgaaagc gacagcggag aaaaatcgca actttaataa agaaaaagta
     3121 atcaacagcc ccaggttgct tggtttatgg cacctgatga agggctatca cttcaactac
     3181 ctggtggcga cggtgttttt agctgtggca gcattcgcgc gcgctggtat ttacctgcac
     3241 ctgggtaatt tcattgatgc tttacgcagt agcgctggat tacacagcaa agccatcatt
     3301 acttcagctt tggctttcct ggggctgatt atgctacagg cattgagcac cttcatgagc
     3361 agctggatgg ccagtttcgc cgctgaaaac accacgcgtc gcttgcgtga tgtgcttttt
     3421 gaccacattc agcggcttac ctatgcctac cacgccgaag ctaaaactgg cgacctgctg
     3481 gaacgcgcca cctctgacgt tgacaccctg cgccgtttct ttgcagacca ggcgattggc
     3541 atggggcgca tcgtgtttat tttcgtcatc aactttgcgc tcattttccg catgcaggca
     3601 cgtttggcat ggatatccgt tgtgattgta ccggtcacgt tgactatctc aatgatattc
     3661 ttccgcaaac tgcgcaaagc ctacgaagaa taccaggaac aagaagccat catcaccacc
     3721 cacctgcagg aaacgctcag tggggtgcgt gttgtcaaag cattcgcgcg ccaggattat
     3781 gaaaccgaga agttcgcaga aaacaacact gaaaaactgc gtcggggcaa gcgtatgatg
     3841 ttcctgcatt ctatcttttg gcccatttcg gatatcatct gtggcttaca acgtgtgacc
     3901 agcacctatg tcgcggcgag cgcggttatt gaccaaagct tgagcctggg tgagttcgtc
     3961 accttccagg gcttgttaag ctggctcatt tggcctattc gcggtttggg gcgtttgatt
     4021 atcgacactt cgcgggcatt ggtctctttg ggtcgtattt tgattgtttt gaaagcggaa
     4081 gaagaagatt taaccactgc ggtttatcaa ccggaaggcg aaacgaaggg tgccatcacg
     4141 tttgaaaatg tatgcttcgc ctatgaagag aatcagcccg tgctcaagga cgtttccttt
     4201 gaatgtcaac ccggcgatgt catcgcgttg cttggctcaa ccggcagtgg caagacttcg
     4261 ttggtcaatc tcttaccccg cttttatgac gtgaacagtg ggcagatcct gctggatggc
     4321 gtcaacctta atgattattc acgcgattac ttgcgctcgc agattggcat cgttgagcag
     4381 gagcctttcc tgttttcctg ctccattcgc gataacatca cctatggtat gcgtcgtgag
     4441 gtgagccagg a
//
LOCUS       DAOSFC010000102         4428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_17797_length_4428_cov_4.737937, whole genome shotgun sequence.
ACCESSION   DAOSFC010000102 DAOSFC010000000
VERSION     DAOSFC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17797_length_4428_cov_4.737937"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /gene="ffh"
                     /locus_tag="PK791_03600"
     CDS             <1..1162
                     /gene="ffh"
                     /locus_tag="PK791_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOH92431.1"
                     /translation="KERAIGAEVSKALNPAQQVIKIVNEELIATLGQAEPLNLRGEKP
                     HVIMMIGLQGSGKTTATAKLARRLRADGERVLMVAADIYRPAAIKQLQTLGERVDVPV
                     FTLDGAKPVKIVQEAYAHAKRGGYSVMLIDTAGRSQLDDFLMQEIDGIQKAVSCNEIL
                     LVIDSMIGQEALNIAEGFRDVMRITGLIFTKIDGDARGGAAISIREVTGVPIKFLGTG
                     EGMDALEVFDPARISGRILGMGDMLGLIEKAEAAYRDEIEIEDTERLLKGQFTLEDFA
                     KQLKQIKKMGPLSQVMGMLPGQFGQVAKHVDPREVDSQLKGVEAIISSMTPAERRDPR
                     ILNASRRRRIAAGCGKNVQDVNRLMKQFREIQNLMKKFQKPGNHRDLERLFG"
     gene            1389..1736
                     /gene="rpsP"
                     /locus_tag="PK791_03605"
     CDS             1389..1736
                     /gene="rpsP"
                     /locus_tag="PK791_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOH92432.1"
                     /translation="MVRIRLRRVGSTHQAHYRVVVADKESPRDGRFIEIVGHYNPRTK
                     PGTIEFKEDRIFYWLGVGAQPSETVEKLFKQVGLDERFKRYKAGEDVETLLAEAEETY
                     KNRVTNTRTTHND"
     gene            1753..2004
                     /locus_tag="PK791_03610"
     CDS             1753..2004
                     /locus_tag="PK791_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOH92433.1"
                     /translation="MDYKKLHDLLEFIAKGLVNDPESVKVDSSIEENTVHLKLSVATE
                     DIGRVIGKQGRVANSIRHLLRAAASREGMQVIMDVVEPE"
     gene            2004..2555
                     /gene="rimM"
                     /locus_tag="PK791_03615"
     CDS             2004..2555
                     /gene="rimM"
                     /locus_tag="PK791_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOH92434.1"
                     /translation="MLGDANNVKNEKAGSAKHEPAFVLVGKLQRPHGVYGEIAMRVDT
                     DFPERIRRGKTLYLGEDCFPVKVLRTRWKGKLLLLKLDGYNSPEDAADLTNLLAFVSV
                     DDIPPLPEGEYYHHQLIGLNVYAGEEFLGVLTSVLQTGANDVYIVEDAAGKELLLPSI
                     PDVILGIDLTSKRMDVHLMEGLR"
     gene            2557..3651
                     /gene="dprA"
                     /locus_tag="PK791_03620"
     CDS             2557..3651
                     /gene="dprA"
                     /locus_tag="PK791_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOH92435.1"
                     /translation="MQERDAWLGFSHVRGMGAVRFRKLLQFFGTLSVAWQAPDDALKV
                     AGLTDKVIQTFRETKRSFDPVAVQSRLQSKGISVLTWNDEKYPPYLKQIAQPPPVLYC
                     LGVFSEADNLAMAIVGTRNVTSYGKQLCHDTATYLAGHGVTIVSGLARGVDAIAHRAA
                     LEIGGRTIAVLGSGVDVIYPPEHRKLAEAIIKSGVVISDYPPGTKPEGINFPPRNRII
                     SGLSRGTIVIEAGERSGALITAKFAVEQGRDVFAVPGSVLSAMSKGTNNLIGEGAIPM
                     TNPADVISHLGLEKQKIQVQEKMPELSPVENRIYQALGSGTVHVDELCAKLDMPIEKL
                     NATITMMQLKGLVVQENNQEYRAVKPWQIG"
     gene            3760..>4428
                     /locus_tag="PK791_03625"
     CDS             3760..>4428
                     /locus_tag="PK791_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOH92436.1"
                     /translation="MKCKEKREIPETTAVFTANGLPGTRGVCPVCGTNVFKMGATPAH
                     EGMEKPVVVKKASKSGAKSSRSTKGGKKSSSKSSQSGRSARGANFADRISMDGLGKPL
                     VIVESPAKAQTIGRFLGNKYKVVASYGHVRDLLASRLSVDPENNFEPEYRVPNDKSKL
                     VKKIAEIAEKSPEVYLATDPDREGESIAWHLMESADIPEEKTKRVVFHEITKPAIDAA
                     FKNAR"
BASE COUNT         1229 a          897 c         1203 g         1099 t
ORIGIN      
        1 taaagaacgt gccatcggcg cggaagtttc taaagcgctt aacccagccc agcaagttat
       61 caaaatcgtg aatgaagaac tgattgccac acttgggcaa gccgaaccct tgaatttgcg
      121 tggcgaaaaa ccacatgtga tcatgatgat tggtttgcag ggttccggta aaacgacagc
      181 gaccgcgaaa ctggcaaggc gattacgcgc ggacggtgag cgcgtactga tggtggcagc
      241 ggacatctac cggccagccg caatcaaaca gctgcaaaca cttggcgaac gtgttgatgt
      301 gccggtgttt acgcttgatg gcgcaaaacc ggtcaaaatt gttcaggaag cctatgccca
      361 cgccaaacgg ggtggctatt ccgtgatgct tattgacacc gctggacgtt cgcaactgga
      421 cgattttttg atgcaggaaa ttgatggaat ccaaaaggct gtttcctgta atgaaatttt
      481 gctggtaatc gactcgatga tcggtcagga agccttaaac atcgcggaag gctttcgcga
      541 tgtgatgcgt atcactggct tgatttttac aaaaattgat ggcgacgcac gcggtggggc
      601 agcgatttca atccgggaag taaccggtgt gcctatcaag ttcctgggta caggcgaggg
      661 tatggatgct ttggaagttt ttgaccccgc gcggattagt gggcgcattt taggcatggg
      721 cgatatgctc ggtttgattg aaaaagccga ggcggcttat agggatgaaa ttgagattga
      781 agataccgag cgattgctga aaggacagtt cacgctggag gattttgcca agcaacttaa
      841 gcagattaag aaaatgggac cgctttcgca agtgatggga atgttaccag gtcagtttgg
      901 gcaagttgcc aagcatgttg acccgcgtga ggtagacagt cagctgaaag gggttgaggc
      961 gattattagt tcaatgacgc cagcggaacg tcgcgacccg cgtattttaa acgccagccg
     1021 gcgaaggaga atcgccgcgg gatgtggcaa aaatgttcag gacgttaatc ggctgatgaa
     1081 acaattccgt gaaatacaaa acctgatgaa aaagttccaa aaaccaggga atcacaggga
     1141 ccttgaaaga ttatttggat agctaaaacg cttgatgtga tttatatttg attagttgtt
     1201 ttgtataaac atgatattga aaaatgagat gaatcaaacc ttacaatatc ttgcaaagcg
     1261 tggtaacatt gttaggttat ttggtaaacc tgagcttgag gagaaggaca atgccccttc
     1321 agcattctct cagcctttta caagagcacg ggccaaagag atttaaaata cttaaggagt
     1381 aaaaatcgat ggtacgtatt cgactacgaa gggtcggttc tacacaccaa gcccattacc
     1441 gtgtggttgt cgctgacaaa gaaagcccac gggatggacg ctttattgaa atcgttgggc
     1501 attacaaccc acgcacaaaa ccaggaacaa ttgagtttaa ggaagaccgt attttctact
     1561 ggttaggcgt tggcgcgcaa ccttctgaaa cagttgaaaa actgttcaaa caggttgggt
     1621 tggatgaacg tttcaagcgc tataaagctg gtgaagacgt ggaaacactg ttagctgaag
     1681 cagaagagac ctataaaaat cgggtcacaa acacccggac aacacataac gattagtttt
     1741 tagaaggatt ttatggatta caagaaacta catgatttat tagagttcat cgccaagggt
     1801 ttggtaaatg accctgagtc tgttaaggtt gatagctcta tcgaagaaaa cactgtgcat
     1861 ttgaagcttt ctgtggcaac cgaagatatc ggacgcgtta tcggtaagca aggccgtgtt
     1921 gcaaactcaa ttcgacatct cttgcgcgct gccgcatctc gcgaaggtat gcaagttatc
     1981 atggacgtgg ttgagcccga ataatgctcg gcgacgcaaa taatgtaaaa aatgaaaaag
     2041 caggctcggc taaacacgag cctgccttcg tattggtagg aaaacttcag cgtccgcacg
     2101 gtgtatatgg cgaaattgcc atgcgggtgg atacggactt cccagaacgc attcgacgtg
     2161 gcaaaacttt atatttgggt gaagactgtt ttccagtcaa agttttgcgg acgcgctgga
     2221 aaggaaaatt gttattattg aaactagatg gttataacag tccggaagat gcggctgatt
     2281 tgaccaacct tttggctttt gtcagtgtgg atgatattcc gcctttgcca gagggtgagt
     2341 attatcacca tcagctgatt ggtttgaatg tttatgcggg ggaagaattt ttgggcgtgc
     2401 tcacatcggt tttacaaact ggtgcaaatg atgtttatat cgttgaagat gcagcgggga
     2461 aagaactgct tttaccgtct atcccagatg tcatattggg aattgatctg acgagcaaac
     2521 ggatggacgt gcatttgatg gaaggtttgc gctagcatgc aagaacgcga tgcctggctg
     2581 ggctttagcc atgtgcgtgg catgggtgct gtgcgtttta gaaagttgct ccagtttttt
     2641 ggaacgctgt ccgtcgcatg gcaagcgcct gatgacgccc ttaaagtagc cggtttgacc
     2701 gacaaggtta tacaaacttt tcgggaaacc aaacggtcgt ttgaccctgt cgctgtacaa
     2761 tccaggcttc agtccaaggg cattagcgtg ctgacctgga atgatgaaaa gtacccacct
     2821 tacctaaagc aaattgctca gccgccaccg gtgctttact gcctgggcgt gtttagcgaa
     2881 gcggataatc ttgccatggc gattgtaggt acccgtaacg taaccagtta tggcaaacag
     2941 ctttgtcatg atactgccac gtatttggct ggacatggcg tgaccatcgt gagtggtctg
     3001 gcacgtggtg tggatgcgat agcgcaccgc gcggctttgg agattggcgg gcgtacgatc
     3061 gctgttttgg gttcaggagt ggatgttatt tatccaccgg agcatcgcaa attggctgag
     3121 gcaattatca agtccggggt cgtgatcagc gattatccgc cagggacgaa gcctgaaggc
     3181 attaatttcc cgccacgtaa ccgaatcatt tcggggcttt cgcggggaac aattgtgatc
     3241 gaagcaggag agcgcagtgg cgccttgatt accgccaaat tcgcggttga gcagggcagg
     3301 gatgtttttg cggtgccggg ttcggtttta tccgcgatga gtaaaggcac aaacaatttg
     3361 attggcgaag gcgcaatccc gatgactaat cccgcagatg tgatcagcca tttgggtttg
     3421 gaaaaacaaa aaatccaggt acaagagaaa atgcctgaac ttagtcctgt cgaaaatcgc
     3481 atctatcaag ccctgggaag tggcacggtg catgtggatg agctttgcgc gaagctggat
     3541 atgccgattg aaaagctgaa cgcgaccatc acaatgatgc aattaaaagg gctggttgtg
     3601 caagagaata atcaggaata tcgcgcggta aaaccctggc aaattggcta attaacacaa
     3661 aaatcaataa ttaaagactt gacttatttt tgaatatggt attatagggc gtatttaggt
     3721 atgggaatat tgcccggagt tattatggaa atatattgtt tgaagtgtaa agaaaaaaga
     3781 gaaatacccg aaacaacggc tgtttttacc gcgaatggac tgcctggtac ccgtggtgtt
     3841 tgtccagttt gtggcacgaa tgtgttcaag atgggtgcaa ccccagccca tgagggtatg
     3901 gaaaagcctg tggtcgttaa aaaagccagt aaatcaggtg caaagagttc gcggagtaca
     3961 aaaggcggta aaaagtcttc atccaagagc agccaaagcg gaaggtccgc gcggggagcc
     4021 aactttgctg atcgaattag catggatggt ttaggcaagc ctttggtgat tgtcgagtcg
     4081 cctgccaaag cccaaaccat cggacgcttt cttggaaata agtacaaggt tgtcgcttcg
     4141 tatgggcatg tgcgcgattt attggcatcg cgtttgagcg ttgatcccga aaataatttc
     4201 gagcccgaat atcgtgtgcc gaatgacaag tcgaaactgg tcaagaaaat tgccgaaatc
     4261 gctgaaaaaa gtcctgaggt ctacctggca actgaccccg accgcgaagg tgagtcaatc
     4321 gcctggcatt tgatggaatc cgcggatatc cccgaagaaa agaccaaacg cgttgttttt
     4381 catgagatta ccaagccagc tattgacgca gcttttaaaa acgcgcga
//
LOCUS       DAOSFC010000103         4416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_17854_length_4416_cov_5.001376, whole genome shotgun sequence.
ACCESSION   DAOSFC010000103 DAOSFC010000000
VERSION     DAOSFC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17854_length_4416_cov_5.001376"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /gene="der"
                     /locus_tag="PK791_03630"
     CDS             <1..996
                     /gene="der"
                     /locus_tag="PK791_03630"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOH92437.1"
                     /translation="SMDFYSLGLGEPYAISAIHGTGTGDLLDAVVAHFPHAIDDDTED
                     ESIKVAIVGKPNAGKSSLLNKIVGQERMIVSNIPGTTRDSIDTRFTYEDQEFTIIDTA
                     GIRKRGSISPGVEKFSVLRAMQSIERCDVAILVIDAVSGITAQDTHIAGFIKDAWKSV
                     ILAVNKWDLIEKDSYTAIEFENRIRQELNFLPYVPIVFISAETGQRVHQILPLVMEVQ
                     KERLYKMSTSQLNKILVEAQDKHQPPGGTGKPFHIYYGTQVRSEPPTFMLYCNDPKLG
                     HFTYLRYLENQIRQVYPFLGTPVRLVLKKRERTERPERPKSGKPKERGTSRKKKA"
     gene            1054..1797
                     /gene="lepB"
                     /locus_tag="PK791_03635"
     CDS             1054..1797
                     /gene="lepB"
                     /locus_tag="PK791_03635"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH92438.1"
                     /translation="MTENNTEKTNQAEETVLQIPTEIVETQSEVDALDITAAEENQKG
                     EKPQSSEKAFWKDLIQTIVMAVVIFLVLNTFTSRVKVFNVSMQPTLKQGYLIVVSKMA
                     YKFGSPKRGDIIVFHHAGVEDQDYIKRVIGLPGDVVEISGGVVSINGTPLTEPYIKEM
                     PRYTLTETVPEGKLFVLGDNRNQSDDSHSWGFVDFDWVVGKALFVYWPLDKIKLLTGP
                     DLLENGTNPNMGSENPPQSFLPALGHFAW"
     gene            1808..2113
                     /locus_tag="PK791_03640"
     CDS             1808..2113
                     /locus_tag="PK791_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOH92439.1"
                     /translation="MTDINLPKNPETCPNCYLGRISRQTRSMVVMHNGKPLTFTAFPA
                     WACDVCHAFVYDPQALIELRLLLGANNLNSVESKAGKRAVKRRKMNKTRKAKTVDPK"
     gene            2366..3445
                     /locus_tag="PK791_03645"
     CDS             2366..3445
                     /locus_tag="PK791_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOH92440.1"
                     /translation="MNAILLSRIIGAIVLGIVGAIFGQPIFEKIQTCLNFTANPSPGL
                     IVAVSAAVFFVIGFIITPCLTIKPIQGLKKLFKKASAQALLSGMAGLLIGFIASAITA
                     YPLSLLPKPFGSIMPFVALVLFGYLFSILLASRNEDIVAALRSFNDRRPLRGKDGSGA
                     NARKILVDTSAIIDGRIVDIAETGFLGGSLIIPRFVLNELQFVSDSSDSLRRQRGRRG
                     LETLTELQENKIVPITISDLDVEGVREVDDRLVILARQMNCPILTNDFNLNRVAELQG
                     VEILNINDLANAVKVVLLPGEGFTVKIIQEGKEYSQGVGYLDDGTMVVVENGEHYMNE
                     ELYVTVTKILQTSAGRMIFAKPDNE"
     gene            3455..>4416
                     /gene="cysS"
                     /locus_tag="PK791_03650"
     CDS             3455..>4416
                     /gene="cysS"
                     /locus_tag="PK791_03650"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOH92441.1"
                     /translation="MSLKIYNTLSRSKEEFEPIEADKVRFYVCGPTVYAEAHLGHAMS
                     ALVFDIVRRYLMYSGYDVNFVMNFTDVDDKIIAKANEIGADPYELAEHYINEYKRNLA
                     ELNILPATKNPRATEDMPQIISMIEALIEKGHAYEAHGDVYYRVESKDDYGKLSGRKL
                     ASMNAGARIEVDERKEHPMDFALWKAAKPGEPAWDSPWGQGRPGWHIECSAMNMTHLG
                     EQIDIHGGGNDLIFPHHENEIAQTEAVTGKEFARVWMHNGMMSLKSEKMSKSLGNMIT
                     VDEFLADYPADVMRLWVLGGSYRGPLVYSEEVIEQTEQAYKRLL"
BASE COUNT         1246 a          998 c         1054 g         1118 t
ORIGIN      
        1 tcaatggatt tttactccct gggacttggc gaaccttatg ccatctccgc catccatggc
       61 acgggtacgg gcgatttgct tgacgcggtt gtggcgcatt ttccgcatgc catcgatgac
      121 gataccgaag atgaatcgat taaggtcgcc attgtgggta aacccaacgc tggaaaaagc
      181 agtttgctaa acaaaattgt cgggcaggag cgcatgattg tcagcaacat tcctggcacc
      241 acccgcgatt cgattgacac aagattcaca tatgaagacc aggaatttac aataatcgac
      301 acagccggta ttcgcaaacg cggttcgatt agtccgggtg tcgaaaaatt ctccgtgctt
      361 cgcgcgatgc aatcgattga acgctgtgat gttgctatcc tggtgattga cgcggttagt
      421 ggcattaccg cccaggatac ccatatcgcc ggctttatta aagacgcatg gaaaagtgtt
      481 attctggcgg tgaacaaatg ggatctaatc gaaaaagaca gctatactgc gattgaattt
      541 gaaaaccgta tcaggcagga actcaacttt ttgccctacg tgcctattgt gtttatctca
      601 gcggaaactg gccaacgcgt gcaccaaatc ctgccactgg tgatggaagt tcaaaaagaa
      661 cgcctgtata agatgagcac gtcgcaactg aataagattt tggtcgaagc gcaggacaaa
      721 catcagccac ccggtggcac aggtaagcct ttccatatct attatggcac ccaggtgcgc
      781 agcgaaccac ctacttttat gctttactgt aacgacccaa aactgggaca tttcacttac
      841 ttgcgctatc tggagaatca aatcaggcaa gtctatccat ttttgggcac tcctgtccga
      901 ctggttctga aaaaacgtga acggaccgaa cgccctgagc gaccgaaaag cgggaagcct
      961 aaggaacgtg gcacaagtcg caagaaaaaa gcttaaacca aatagtgtat aatagcgcgt
     1021 taatgcttat aatgtatagc gattggaata attgtgacag aaaataacac cgaaaaaaca
     1081 aatcaagctg aagaaacagt ccttcagata ccgacagaga ttgttgaaac gcaatccgaa
     1141 gtggacgcgt tagatatcac cgctgccgaa gaaaaccaga aaggcgaaaa accccaaagt
     1201 agcgaaaaag ctttctggaa ggaccttatc caaacgattg tcatggctgt ggtaatcttc
     1261 ctggtcttga acacctttac atcgcgggtg aaagtcttta acgtcagcat gcaacccacc
     1321 ttgaagcaag gttatctcat tgtagtcagc aaaatggctt ataaatttgg ttcccccaaa
     1381 cggggcgaca ttattgtctt tcatcacgcc ggcgtagaag atcaagacta catcaaacgc
     1441 gtgattggct tgcccggcga cgtggtagag attagtggcg gtgtggtgag catcaatggc
     1501 acacccttaa ccgaacctta cattaaggaa atgccacgtt atacactcac cgaaacggtg
     1561 cccgaaggca aactcttcgt gttgggcgat aaccgcaatc agtcagatga ttcacatagc
     1621 tggggctttg tggattttga ctgggtggtc ggaaaagctt tgtttgtcta ttggcccttg
     1681 gataaaatca aactgttaac cgggccagat ttgcttgaaa acggcacaaa cccgaacatg
     1741 ggcagcgaga atcccccgca aagtttttta cctgccctgg gtcactttgc ctggtaaatt
     1801 gaaggaaatg acggatataa acttgcccaa aaaccccgaa acttgcccga actgttactt
     1861 gggcagaatt tcccgccaaa cgcgcagtat ggttgtgatg cacaatggta agccacttac
     1921 ttttactgca tttcccgcgt gggcttgcga tgtatgccat gcctttgttt atgacccgca
     1981 agctttgatt gaactaagac ttttgctggg cgcgaacaat cttaattcag ttgagtctaa
     2041 ggcgggaaaa cgcgcggtaa aaaggcgcaa aatgaacaaa acgcgtaaag ccaaaaccgt
     2101 tgacccaaaa taggcagaca cacacaataa atagcatagc cacttttctg cttgccaacc
     2161 tgaagtggca tttttcaaaa taaaagagaa ttcacaaaaa tcatcttcca ggtgataagt
     2221 gtcatgaaaa tgagacattc tcttaaagta atgttagaat gatttgttat catttgacta
     2281 tgaattttca aagtgctatg atatgaatgc ataaggaatt ataaattatt tattttgttc
     2341 attaacatcg gaggtagatt ttattatgaa cgcgatttta ttatctcgta ttattggcgc
     2401 gattgttctc ggtatcgtag gcgcaatttt tggccaacct atatttgaaa aaattcaaac
     2461 ttgtctcaac ttcacggcaa acccaagtcc tggcttgatt gtggctgttt ccgctgctgt
     2521 gttttttgtc attggcttta tcatcacacc ttgtttaacc atcaagccta tccagggctt
     2581 aaagaaactc tttaaaaaag ccagcgcaca agccttgctc agtggtatgg cgggtctatt
     2641 gattggtttt atagcgtcag caattacagc ctatccttta tcgttgcttc ccaaaccttt
     2701 tggctcgatt atgccatttg tagccctggt tctttttggc tatttgtttt ccattttgtt
     2761 ggcatcgcgc aatgaagaca tcgtagcagc cctgcgaagc ttcaatgaca gacgcccatt
     2821 gcgtggcaaa gatggcagtg gagcaaacgc gcgaaagatt ttggtggata ccagcgccat
     2881 tattgatgga cgcattgtgg atatagccga aaccggtttt ctgggtggca gtttgattat
     2941 cccccgtttt gtgctcaatg agttgcagtt tgtatcggat tcttccgaca gcttacgccg
     3001 tcaacgtggc cgacgtggtc tcgaaacgct gactgaactc caggaaaaca aaatcgtgcc
     3061 cattacaatc agcgacctgg atgttgaagg tgtgcgcgaa gttgatgatc gcctggtaat
     3121 tttggcgcga cagatgaatt gccccatctt aaccaacgat ttcaacctaa accgcgtggc
     3181 agaactgcaa ggagtcgaaa ttttgaacat caacgatctg gccaatgcgg taaaagttgt
     3241 cttattgcct ggtgaaggct ttacagtaaa aattatccag gaaggcaagg aatacagcca
     3301 gggcgttggt taccttgacg atggcacgat ggtagtggtt gagaacggtg aacactatat
     3361 gaacgaagag ctttatgtaa cggtcaccaa aatcctgcaa acttcggcag gacgcatgat
     3421 ttttgccaag cctgacaatg aataggaggt ttagatgtcc ctgaaaattt acaacaccct
     3481 gagtcgttca aaagaagaat ttgagcccat tgaagctgac aaagtgcgct tttatgtctg
     3541 cgggcctacg gtctatgcag aggcgcatct cgggcacgcc atgtcggctt tggtctttga
     3601 tattgtacgg cgttatttga tgtactccgg ctatgacgtg aattttgtca tgaatttcac
     3661 cgatgttgat gacaaaatca tcgcaaaagc aaatgaaatc ggcgctgacc cctatgaact
     3721 ggcggagcat tatatcaatg agtacaaacg caatctggca gaattgaaca ttttgcccgc
     3781 gaccaaaaat ccacgtgcca ccgaagatat gccccagatt attagcatga ttgaagcttt
     3841 aatcgaaaaa gggcatgcct acgaggcgca tggcgatgtc tactaccgtg tcgaatccaa
     3901 agatgattat ggcaagcttt ccggacgcaa gctggcaagc atgaacgccg gcgcgcgcat
     3961 tgaagtggac gagcgcaaag agcaccccat ggactttgcc ttgtggaaag ccgccaagcc
     4021 cggtgagccc gcctgggaca gcccctgggg acaaggccgg cccggctggc atattgagtg
     4081 ctccgcgatg aacatgaccc atctcggcga gcaaattgac attcatggcg gtggaaatga
     4141 ccttatcttc ccgcaccacg aaaacgaaat cgcccaaacc gaagccgtaa ccggcaagga
     4201 attcgcgcgg gtgtggatgc acaatggcat gatgagcctc aaatccgaaa agatgtcaaa
     4261 atccctgggt aatatgatta ccgtcgatga attcctggct gattacccag cggatgtcat
     4321 gcgtttgtgg gtcttgggtg gttcataccg cggaccatta gtttacagtg aagaggttat
     4381 tgaacaaacg gaacaggcat acaaacgctt gttaag
//
LOCUS       DAOSFC010000104         4404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_17917_length_4404_cov_3.994022, whole genome shotgun sequence.
ACCESSION   DAOSFC010000104 DAOSFC010000000
VERSION     DAOSFC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17917_length_4404_cov_3.994022"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK791_03655"
     CDS             <1..388
                     /locus_tag="PK791_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOH92442.1"
                     /translation="KEEKTADVRGRNLITGLPEAIEVSSIEIREALAPSVQVIIDTIK
                     DALDESPPEILADLMDSGICLAGGTSQLQGLAERLANEIRVRVWVAEDPLTCVARGCG
                     MIFDDVEQYEGLLVGIERDPYSSGQS"
     gene            434..1270
                     /gene="mreC"
                     /locus_tag="PK791_03660"
     CDS             434..1270
                     /gene="mreC"
                     /locus_tag="PK791_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOH92443.1"
                     /translation="MRDLFSLKNIRNLVIVTIVFGIIIMAMSGYLTPVFSFIFNPVIS
                     TQSWLSERYVAFRDFFTSPRDMATLRAQNQALEQEVAQLQSEIVALQESLSQSEILFT
                     LLDFARSHPEHEYIAATVIGREISPYLQYVIIDKGSNHGIRYGMPVVTEEGLVGRIDA
                     VIANASRVKLITDASMTVNVRLKTANVDAQTTGSITGEISLGMVPQDAEVQTGDVLLT
                     SGLGGTYPPNIFVGQVLSMQSKQNTLFQTGSVQAVVDFQGLSAVLVISDFNVVDISPL
                     VP"
     gene            1280..1783
                     /locus_tag="PK791_03665"
     CDS             1280..1783
                     /locus_tag="PK791_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92444.1"
                     /translation="MIEYLISPVSFLIAVMLQYGIFSRWTLLSGCVDIVLLFIIAFCL
                     HSNAKRLWLIILFFGAIIGTISALPFYIPLAFYFGVYLLTQQFRKRIMQTPLLSMYLS
                     TFISTLAWHGINIAWLFVKRMPFEFSTAFFEILLPSVLLNMLFSFPVHAITQEISRVI
                     TPKGAEA"
     gene            1780..3894
                     /gene="mrdA"
                     /locus_tag="PK791_03670"
     CDS             1780..3894
                     /gene="mrdA"
                     /locus_tag="PK791_03670"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOH92445.1"
                     /translation="MINNNNNNNQQYEKPTIALNRFRWVFLIIALVFMFYVFTLFDLQ
                     IIQGASFRAKAEENRVRVISIPTQRGMIYDRNGILLARNVPVFHVTITPASLPASEGK
                     TYEIFRELSVLLDMPVSRGEVNDETSALFTPCYTDFGIEQIVEISSTLWPYSATRVKC
                     NVSREVAMQVTERNNDWPGVDIEIDSVREYPTGENTAELIGFLGPIPEVMLDYYTEQG
                     FVSGRDKVGYMGVENSLQSILGGTNGKRVVEVNVGGEILRDIEEPIEPVPGQDVYLTI
                     DYRLQKAAREALISELEIWNQRYLEWRGEILSTTGVVIAMNVKTGEILAMVSIPSYEN
                     NRMANYIPGDYYEQLSVDEAKPLLNKAISSELPPGSVYKMATAIGALNEGVIGPYDTV
                     YDPGVITLEQKFIEGERVTMTQDYVCWYEIGHGDVDFVHGIKWSCNVYFYKIGGGYGD
                     EVADNGLGIWRMNEYAHAIGYGQLTGIELPGEADGLLPNPTWKRLNQGENWATGDTYL
                     AAVGQGYVLATPLQVLHSIATLSNNGQHMQVTLVSKTTDAEGNIISSFEPKMLWDITK
                     DELIESYIGNNKTGEFKAVEPYVLEFAKRGMEEVTYPGGTASDLFEGDDKKVAGKTGT
                     AEYCDDWANREGLCVPGNWPAHAWFVGYAPYDDPEIAVVAFIYNGSEGSAVSAPIVRR
                     VIDFYFEQKAIDEALALPGGGH"
     gene            3891..>4404
                     /locus_tag="PK791_03675"
     CDS             3891..>4404
                     /locus_tag="PK791_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92446.1"
                     /translation="MSQKPELTIKGYRGGVLITLPAVSCFAQRDLLIRRIQSQERFFK
                     GGRIALDVGETAWNEEQISRLLRDLSDEGVCLWAILSTSQVTLDSAANYDIPTSIKRD
                     GEPKGQTETSPAQPDLPKTTWLETNISADLPKQERHFEGKLCLIGDVKPGASLSADDS
                     ILIWGKAAGQI"
BASE COUNT         1109 a         1131 c         1036 g         1128 t
ORIGIN      
        1 gaaagaagaa aaaaccgccg atgtgcgtgg acgtaactta attactggtt tgcctgaagc
       61 catagaagtc tcatcaatcg aaatccgcga agctttggcc ccttcggtgc aggtgattat
      121 tgacactatc aaagatgcgc ttgacgaaag cccgcctgaa attttggcag acttgatgga
      181 ctcgggcatc tgtctggcag gcggaacatc gcaactacaa gggctggctg aacgccttgc
      241 caacgaaatc cgcgtgcgag tttgggttgc tgaagacccc ttaacctgcg tagcacgtgg
      301 ttgtggcatg atttttgatg acgttgaaca atacgaagga ctcttggtgg gtatcgaacg
      361 cgatccctat tcaagcggtc agtcataatt cgcatatccc cttgtgggat tgttttgcac
      421 aggttctttt aatatgcgag acctattttc acttaaaaac attcgtaacc ttgttattgt
      481 aaccatcgta tttggcatca tcatcatggc gatgtcgggc tatttaacgc cagttttcag
      541 ctttatcttc aaccccgtca tctcaacgca aagctggcta tcggaacgtt atgtcgcatt
      601 tcgcgatttt ttcacatctc cccgcgatat ggcaaccctg cgcgcccaaa atcaggcgct
      661 ggaacaggaa gtagcccaac tgcaaagcga gattgttgcc ctgcaggaaa gtctcagcca
      721 atctgaaatt cttttcacct tgcttgattt tgcccgttcc catcccgaac acgaatacat
      781 cgccgcaacc gtcatcggcc gcgaaattag tccctatctt cagtacgtca tcattgacaa
      841 aggttcaaac cacggcatac gctatggcat gccagttgtt accgaagaag gattggtagg
      901 acgcattgac gccgtcattg ccaacgcctc gcgtgtgaag ctcatcaccg atgctagtat
      961 gaccgtcaat gtacgcctga aaacggccaa tgttgacgcg caaaccacgg gttcaatcac
     1021 gggcgaaata tcgctgggca tggttccgca agacgcagaa gtacaaactg gtgatgtcct
     1081 gcttaccagt gggcttggag gcacctaccc accaaacatt tttgtgggtc aggtactctc
     1141 gatgcaatcc aagcaaaaca cgctttttca aacgggttct gtgcaagcgg tagttgattt
     1201 tcaaggcctg agcgctgtgt tggtcatctc cgacttcaac gtcgtggaca tcagcccttt
     1261 ggtgccctaa ggaacttgca tgattgagta cctaatcagc cccgtcagct tcttaattgc
     1321 cgtcatgttg cagtatggca tttttagccg ctggacctta ctctcgggtt gcgtggatat
     1381 cgtcttattg ttcatcattg ccttttgtct ccactccaac gcaaaacgcc tgtggcttat
     1441 tattttgttt ttcggcgcga ttattggcac catatccgca ttaccctttt atatacccct
     1501 ggccttttac ttcggagtgt atcttctgac ccaacaattc cgcaaacgca ttatgcaaac
     1561 gccattactc agcatgtatt tatcgacttt tatcagcacc ctggcatggc atggcattaa
     1621 cattgcctgg cttttcgtca aacgcatgcc gtttgagttt tccaccgcgt ttttcgaaat
     1681 cttactgcca tcagttctat tgaatatgct gttttcattt cctgtccacg ccatcaccca
     1741 agagattagc cgcgttatca ctccgaaagg agccgaagca tgattaacaa taacaataac
     1801 aataaccaac agtacgaaaa accaaccatc gccctaaacc gcttccgttg ggttttcctg
     1861 attatcgccc tggtttttat gttttacgtc tttacgcttt ttgacttaca aattatccag
     1921 ggtgccagtt ttcgcgccaa ggcagaagaa aaccgcgttc gtgtaatcag catccccacc
     1981 cagcggggta tgatttatga ccgcaacggc atccttctcg cccggaacgt gcctgtattc
     2041 catgtaacca ttacgccggc cagtttgcca gcctcagaag gaaaaactta tgagattttc
     2101 cgtgaactga gcgtgctgct ggatatgccc gtgagtcgtg gagaagtcaa tgatgaaact
     2161 tccgccctgt ttacaccatg ttacaccgac tttggcatcg agcaaatcgt ggaaatttcc
     2221 agcacacttt ggccttattc agccacgcgg gttaaatgta acgttagcag agaagttgcc
     2281 atgcaagtca ctgaaagaaa caacgactgg cctggcgttg acatcgaaat cgattccgtt
     2341 cgcgaatacc ctaccggcga gaacaccgct gaactcatcg gctttttagg tcccattcct
     2401 gaagtaatgc ttgactatta caccgagcaa ggcttcgttt ccggacgtga caaggtcggt
     2461 tacatgggcg tggaaaactc cttgcaatct atcctgggtg gcaccaacgg gaaacgcgtt
     2521 gttgaagtca atgttggtgg cgaaattctc cgcgatattg aagaacccat cgaaccagtt
     2581 ccaggccagg acgtttacct gaccattgat taccggttgc aaaaagccgc ccgcgaagct
     2641 ttaatctccg aactggaaat ctggaaccaa cgctacctgg aatggcgtgg cgaaattctg
     2701 agcacaacag gtgtggttat cgccatgaac gtgaaaaccg gtgagatttt agctatggta
     2761 tccatcccaa gttacgaaaa caaccgcatg gcaaattata ttcctggtga ttattatgaa
     2821 caacttagtg tggacgaagc caagccgctg cttaacaaag ccatttcctc cgaattgccc
     2881 cctggttctg tttacaaaat ggcaaccgcc atcggtgcct taaacgaagg tgtcatcggc
     2941 ccctacgata cagtttatga ccctggggtt atcacactgg aacaaaagtt tattgaaggc
     3001 gaacgcgtaa ccatgaccca ggattatgtc tgctggtatg agattggcca cggtgatgtt
     3061 gattttgtgc atggtattaa atggtcttgt aatgtctatt tttacaaaat tggcggtggc
     3121 tatggcgacg aagttgccga caacggactg ggcatttggc gcatgaacga atacgcccat
     3181 gccatcggtt atggacaact gaccggtatc gaattgcccg gcgaagctga tggccttttg
     3241 cccaatccca cctggaaacg cttaaaccag ggcgaaaact gggctactgg tgacacttat
     3301 ctcgcagctg tcggtcaggg ttatgtgctg gcaacacctt tgcaagtctt gcattctatc
     3361 gcgacacttt ccaataatgg ccaacacatg caggtcaccc ttgtttctaa aacaaccgac
     3421 gcggaaggca atatcataag cagttttgaa cccaaaatgc tttgggacat caccaaagat
     3481 gaactcatcg aaagctacat tggcaacaac aaaaccggcg aattcaaagc tgttgaaccc
     3541 tatgtcctgg aatttgccaa acgtggcatg gaagaagtga cctaccctgg cggtacggca
     3601 tcggacctgt ttgaagggga tgataaaaaa gtcgccggca aaaccggtac cgcggaatat
     3661 tgtgacgact gggcaaaccg cgaaggcctt tgcgtgccgg gcaactggcc cgcccacgcc
     3721 tggtttgtgg gctatgcgcc ttatgatgac ccggaaatcg ccgtggtcgc ttttatctac
     3781 aacggtagcg aaggttccgc cgttagcgcg cccattgtgc gtcgcgttat tgacttttac
     3841 ttcgaacaaa aagctattga tgaagccctg gccctgcccg gaggtggtca ttgagtcaaa
     3901 aacccgagtt gacgattaaa ggctatcgtg gcggggttct aattaccctg cctgcggttt
     3961 cctgtttcgc ccaacgggat ttactcattc ggcgtattca aagccaggaa cgtttcttca
     4021 agggcggtcg catcgccctg gacgttggcg aaacggcgtg gaacgaggag caaatttcca
     4081 gacttttacg cgatctttct gacgaaggcg tttgcttatg ggctatcctg agcacgtccc
     4141 aggtgaccct ggattcagct gccaattatg acattcccac gtcaatcaaa cgcgatggcg
     4201 agccaaaagg ccaaaccgaa accagccccg cccaacccga cttgcccaag accacctggc
     4261 tagaaacaaa tatctcagcc gatttaccca agcaagaacg ccattttgaa ggcaaactct
     4321 gtcttatcgg tgacgtaaaa ccaggcgcaa gcctgagtgc cgatgattcg attctcattt
     4381 ggggcaaagc agctgggcag atat
//
LOCUS       DAOSFC010000105         4397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_17959_length_4397_cov_3.922156, whole genome shotgun sequence.
ACCESSION   DAOSFC010000105 DAOSFC010000000
VERSION     DAOSFC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17959_length_4397_cov_3.922156"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PK791_03680"
     CDS             <1..447
                     /locus_tag="PK791_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOH92447.1"
                     /translation="IIEAEKHLAKWGEADSISDSRINEDAVTTSIAAKSMAEDKDVTA
                     ICVFTFSGNSARWVSKAKPSVPIYAFTSHISTYTWLGICRGVIPFLVKHSDSLEEMLD
                     LIETELIAIDPNIVGKQVIMVGSFPVGAHASPNLALLHTIKNTEKH"
     gene            complement(517..1569)
                     /locus_tag="PK791_03685"
     CDS             complement(517..1569)
                     /locus_tag="PK791_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH92448.1"
                     /translation="MPSTVEKKVERNLIGLTLSDYAGKKSTLCPGCGHTAIANVIQAA
                     VYELGLAPEQVVKISGIGCSSKSPNYFLNRSFGFNGLHGRMPTLVTGACFADYKIEIL
                     GISGDGDTASIGLSHFKHIIRRNVPMLYIIENNGVYGLTKGQFSATSDQGLVLKHQGE
                     NLLPPLDMCMEALISGAPFVARAFSGDTKQMKEIFKAAIAQEGTAVVDVISPCVTFNN
                     RAEAMQSYTWGRMNQTPLQAIKHFNTKEEIDEETETIEPGTFSEIQLQDGSYLMLRKL
                     DKDYDPSDKAAALDILTESYKKHYFATGLIYFSEVPPTIQERYDLPEEPLNRIGIERL
                     RPTSAMLDKINHEYGI"
     gene            complement(1562..3391)
                     /locus_tag="PK791_03690"
     CDS             complement(1562..3391)
                     /locus_tag="PK791_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOH92449.1"
                     /translation="MAVTELQSTTGLKPIVNDFCMTVCTVNGSGSATANNIIYRALYQ
                     MGIPVSGKNIFPSNIMGMATWFVIRVNDDGFIGRVPHDDIVVAMNPGTLVKETAYLPE
                     GGVIFYADHITVPEGHREDLIYYPMPIKELIKTVDVPRNFVAYLENMLYVGIVSQVLG
                     IELSELRIAVEHQFIGRDAIIESNFGIIKLGYEWAAENVEKRDRFVVERRPFNNNLIM
                     TDGNTAAGIGSLFGGLQFCSWYPITPATSMVEAAIAKVDELRKDPVTGKTTCVILQVE
                     DELAAAGAAVGAGWAGLRSMTSTSGPGICLMAENIGLAYFTETPVVIWNVQRVGPSTG
                     LPTRTAQGDLSFMYNVGHGDINHIILIPGTVNECFEFGWKSLDIAERYQTPIFVLSDL
                     DLGMNTWVTEKFEYPSTPIDRGKLIWEEELEKIEDWGRYKDIDGDGIPYRTVMGNQSP
                     KSAYFTRGTGHDVYGNYNEDPKNWSDMLHRIEKKLNGAIENLPKPIVIKGKDARIGII
                     GMGSTNDAIRETQAELAKQGIETDYMRIRALPSCCEVREFIEEHERTYVLELNRDGQL
                     KEILMLEHSDLSEKLISLTDIGGLPMTAIWAVENILAAEEEYA"
     gene            complement(3407..4087)
                     /locus_tag="PK791_03695"
     CDS             complement(3407..4087)
                     /locus_tag="PK791_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92450.1"
                     /translation="MAQEKQTLLEYLAKASKNGDDIPSIAELSQILGVSIANVREQLE
                     VARQLGFVEVRPRTGIQKRAFSMVPLIRLGMRYGVHSEPKILDDYIDMRKHLELVYWD
                     PAFKVINDNQIDYMQFLVDVAFRKLQRKPAIIPHEEQSEFHLTIFRPLGNKLLINFLE
                     AYWDLETTNSVRFFMDLDDMLSIWSRRQVLVDALREKNAIKGKKALESLLTYPIKPRA
                     AEIKQQFE"
BASE COUNT         1200 a         1042 c          925 g         1230 t
ORIGIN      
        1 atcattgagg ctgaaaaaca tctcgccaaa tggggagaag cggattcaat cagtgattct
       61 cgcatcaatg aagacgctgt cactacgtcg atagccgcaa aaagcatggc cgaagacaaa
      121 gatgttaccg cgatttgtgt tttcaccttt agcggcaact ccgcgcgttg ggtcagcaag
      181 gccaagccca gcgttcccat ctatgctttc acatcccaca taagtaccta tacctggctt
      241 gggatttgtc gtggcgtcat cccttttctc gtcaagcatt cggattccct ggaagaaatg
      301 ctcgacctta tcgaaacgga actcatcgcc attgacccca acattgtggg caaacaagtc
      361 atcatggtcg gcagtttccc cgttggcgcg cacgcttcgc ctaacctggc cttacttcac
      421 acaataaaaa acacagaaaa acactgataa cagaaagaac aagtggctgc ctgaaacaga
      481 cagccacttt taatctcatt aggattttag ataagcctaa ataccatatt cgtgattgat
      541 cttatccagc atcgcgctgg ttgggcgtaa gcgttcaatg ccgatgcggt tcaagggctc
      601 ttcaggtaaa tcatagcgtt cctgaatggt tggcggaact tctgaaaaat aaatcaatcc
      661 cgtggcaaaa tagtgctttt tataggattc tgtaagaata tccaaagcgg cagctttatc
      721 agatgggtca taatccttgt caagcttacg aagcatcagg taagaaccat cctgcaactg
      781 aatctctgag aaagtaccgg gttcaattgt ttcagtttct tcgtcgattt cttccttggt
      841 attaaagtgc ttgattgcct gcaatggcgt ttgattcatg cgaccccagg tgtaggattg
      901 catcgcttcg gcacggttgt taaaggtcac gcatgggctg ataacgtcca caaccgcggt
      961 accttcctgg gcaatggcag ctttgaatat ttccttcatt tgtttcgtgt cgccggagaa
     1021 agcgcgcgcg acaaagggtg cgccactaat cagcgcttcc atacacatat ccaaaggtgg
     1081 caacaggttt tcgccctggt gtttcaatac caagccttgg tcagaagttg ccgagaattg
     1141 tcctttggtc agtccatata caccattatt ttcgatgata tacaacatgg gcacgtttcg
     1201 gcgaataata tgtttgaagt ggctcaaacc gatgctcgcg gtatcgccat caccggaaat
     1261 acccaaaatt tcgattttgt aatccgcgaa acaggcacca gtgaccaggg tcggcatacg
     1321 accatgcaaa ccattgaagc caaacgatcg atttaagaaa tagttggggc tcttactcga
     1381 acacccaata ccactaatct ttacaacctg ctcaggtgct aagccaagtt cataaacagc
     1441 ggcctgaata acgttcgcga ttgccgtatg accacaaccg gggcaaagcg ttgacttttt
     1501 gccggcatag tcgctaaggg tcaagccgat aagattgcgt tcaactttct tttcaacagt
     1561 gctaggcata ttcttcctcc gcagctaaga tattttcaac agcccatata gccgtcatcg
     1621 gcaaaccacc gatatcggta agagagatga gtttttcgga aagatccgag tgttccagca
     1681 tcaaaatttc cttcagctga ccatcgcggt tcagttccaa tacgtaagta cgctcatgtt
     1741 cttcgataaa ttctcgaact tcacagcagc ttggcagtgc acgaattcgc atatagtccg
     1801 tttcaatgcc ttgcttggcc aattctgcct gtgtttccct gatggcgtcg ttcgtcgatc
     1861 ccatacctat gatgccgatt ctggcatctt tacccttgat aacaatgggt tttggaaggt
     1921 tttcaattgc gccattgagt ttcttttcaa tacgatggag catatcggac cagtttttag
     1981 gatcttcgtt atagttgcca taaacatcat gtccggtacc acgggtaaag taagcagatt
     2041 tggggctttg gttacccatc acggtgcgat atgggatgcc atcaccatca atatctttgt
     2101 aacgacccca gtcttcaatc ttttccagtt cttcttccca aatcaactta ccacggtcaa
     2161 tgggcgttga aggatattca aacttttctg tcacccaggt gttcatgccc aaatcaaggt
     2221 cactgagcac aaaaatcggg gtctgatagc gttctgcgat atcaagcgat ttccagccaa
     2281 attcaaaaca ctcgtttact gttccaggga taaggatgat gtgattaata tcaccatgcc
     2341 caacattgta cataaaggat aagtcgcctt gcgcggttcg cgtgggcaaa ccggtgctgg
     2401 gaccaacccg ctgtacgttc caaatcacaa cgggtgtttc ggtaaagtaa gccaaaccga
     2461 tattctctgc catcaaacaa atgccagggc ctgatgttga ggtcatggag cgtaagccag
     2521 cccatcccgc gcctactgcg gcaccagcag cggcaagttc atcttcaacc tgcaaaataa
     2581 cacaggttgt tttgcctgta acagggtcct tgcgcagttc atcaactttg gcaatggcag
     2641 cttcgaccat actcgtagcc ggtgtaatgg gataccagga acaaaactgc agaccaccaa
     2701 aaagtgaacc aatacccgcg gcagtattgc catcggtcat gatcaaatta ttattgaacg
     2761 gtcggcgttc cacaacaaag cggtcgcgtt tttcaacgtt ttctgcagcc cactcgtagc
     2821 ccagtttgat gattccaaag ttagactcaa taattgcatc tcggccaata aattgatgtt
     2881 caacagctat gcgcaattcg gacaattcaa ttcccaagac ctgtgaaacg atgcctacat
     2941 acagcatgtt ttccaaatag gctacaaaat tacggggcac atcaacggtt ttgatgagtt
     3001 ctttgatggg catcggatag taaataaggt cttcacgatg gccttcagga actgtgatat
     3061 ggtctgcgta aaaaataacg ccaccttcgg gcaaataggc tgtttctttt accaatgtgc
     3121 cggggttcat cgcaaccacg atatcatcgt gcggaacacg gccgatgaat ccatcatcat
     3181 ttacacgaat aacaaaccat gttgccatac ccataatatt agatgggaaa atgttcttac
     3241 cagatacggg gatacccatc tggtacaatg cgcggtaaat aatattattt gccgtcgcgc
     3301 tacccgaacc attcaccgtg caaactgtca tacaaaagtc gttcacaatc ggcttcagac
     3361 ccgtagtaga ttgaagttca gtcactgcca ttccaggttc tctccttcat tcaaattgtt
     3421 gttttatctc agcagctctg ggtttgatgg gataagtcaa caaactttcg agtgcttttt
     3481 ttcctttaat tgcgtttttc tcacgcaagg cgtcaacaag gacttgtcgc ctgctccata
     3541 ttgaaagcat atcgtccaag tccataaaaa accttacgct gttagtcgtc tccaggtccc
     3601 agtaggcttc caaaaaattg atgagaagct tgtttccgag gggacgaaaa atggtaaggt
     3661 gaaattcgct ttgttcttca tggggtatta tagccggttt tcgctgtaat ttacgaaatg
     3721 cgacgtccac taaaaactgc atgtaatcga tttgattatc attgatgacc ttgaatgctg
     3781 gatcccagta aacgagttca aggtgtttgc gcatatcaat gtaatcatcg agaatttttg
     3841 gttcgctgtg tacaccgtat ctcataccca aacgaatgag tggcaccata gaaaaagccc
     3901 gcttttgaat ccctgtgcga ggcctcacct ccacaaaacc taattgacgt gcaacttcca
     3961 gctgttccct tacgttcgca atacttacgc ctaaaatttg gcttaattcc gcgatgctgg
     4021 ggatatcatc cccgttctta gatgcttttg ccaaatattc gagtaaagtt tgcttttcct
     4081 gtgccataat caattaatat gattaattga attaattcat ctcgattata tgtcagcctg
     4141 tcaaatgcgt caagtattat tgccgattta taatccaatt gtaaattaat atttacggct
     4201 tgaccttgaa acagaaatgt ttcatgaagt tttttatcaa caggaattaa ccattttttc
     4261 atccttccat agtacaataa acgaaataat tgtagcattt acatattccg aatttttgtt
     4321 tcaaggaggt tcaggcgtga tatgcgcaaa ttgtaattcc tacaacgatg acaagaacaa
     4381 gttttgtcgg aattgtg
//
LOCUS       DAOSFC010000106         4385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_18036_length_4385_cov_6.144111, whole genome shotgun sequence.
ACCESSION   DAOSFC010000106 DAOSFC010000000
VERSION     DAOSFC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18036_length_4385_cov_6.144111"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK791_03700"
     CDS             complement(<1..270)
                     /locus_tag="PK791_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92451.1"
                     /translation="MSKNSKFFSKVALLLLILTLLLSNGSIVKANPTPTIQIPDTPLV
                     HEESRNHPNTGSAGIAWADPDGPHSTYTWPHEFAQMGHLISSYQNY"
     gene            568..3009
                     /locus_tag="PK791_03705"
     CDS             568..3009
                     /locus_tag="PK791_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOH92452.1"
                     /translation="MNPKSLILLEYHKILGKLKAYASFSGSEALATRLRPTSSLPKAL
                     TLQTQTREARYLLNFSETVSFAGVADLAPLVGMARNAIVLEAPDLIAIRNTLISARDA
                     KRIIINRADELPHLAGIAEGLTDGAGLIDLINRTITERGEVLDSASPALGQIRGKIKV
                     VHARLIDRLSRYINDANTMKLLQEPLITQRGGRYVLPLRAECKGSFKSIIHDQSASGA
                     TLFVEPVAVVELNNEYRELELAERDEVLRVLRALTLRIAELHDSLLASTRAMAELDLI
                     LMKAKYAEELDAVEPVLITFDEKPAPHHPGSCIRLRRARHPLLDPEKVVPIDVEMDNE
                     NFIIVLTGPNTGGKTVTLKTVGLAVLMAQSGMQIAAQSGSCLSVFRDVFADIGDEQSI
                     EQSLSTFSAHITQLVRILKRADYRSLILLDELGSGTDPQEGSALARAVLTWLIEKRIT
                     SIVATHYPELKAYAHVTKGAVNASLEFDLQTLKPTYRLTIGLPGRSNALAIAERLGLR
                     QEIIDMARQDIDPTELGADDLLDEIRRQRDLTAESYALAERARKDAENTRNQLNRRLD
                     DIENRKDAILEATQEKAEEELEAMKQELRELRRILARLQAAPEKKAELAEVVEKVENI
                     EKRHEKKARQIKRRPRPKSEKGPLKVGDKVIVRKLGAEAVISAIDGADIEVQAGVLRL
                     RLRTEDLRRKVDEEPEEFVSDEAKPSEPKPSQPKPARKGRTRLPTVEMPALELDLRGK
                     RVDDGLDEFQRWLERGFAAGMLFGRVIHGHGTGAMKDAVRAELRQSQFVKRWEPGGEK
                     EGGDGVSVVFFNHQT"
     gene            complement(3105..4334)
                     /locus_tag="PK791_03710"
     CDS             complement(3105..4334)
                     /locus_tag="PK791_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92453.1"
                     /translation="MKIRKSLFVLIVMIVLISLAGCGQNQDALQTHDRPAQERDNNLS
                     PETGPTESEPEPTATPAEPEFDVARAYLDTLEYFYQFIVSGSTDFDYEYGTVGIGEQA
                     SFRTPDEALESTGFAITDLSGDGLPELFIGEIAADSQAVSLLAPAYAVFALQDNEPAL
                     TFTGWYRNAYFYHGDGRFYQTGSNGAMYSIEGIYKLSPDGLTLNCEHYFFTYEKDETL
                     SEIGNYYNTSCQSDKAVSVELSDEELAEKRKELASEPVSVELIPFAEYPRWKEEHGFG
                     QITTDTTNLLNAIRVDYASDELLANPGTYDEVIIDASDSLAKVVFMANITVKDFKYLE
                     ILLTDCVGDECSFDIVEELYSMNELRPERPLVIGMVFMGSIPNRGISFVDETGTTRYF
                     SINQSGRDGNLFLLEFE"
BASE COUNT         1073 a         1080 c         1167 g         1065 t
ORIGIN      
        1 ataattttga taagaactaa tcaagtgtcc catttgcgcg aactcatggg gccaggtgta
       61 ggttgagtga ggcccatccg ggtctgccca ggcgatgcct gccgagccgg tatttggatg
      121 attcctgctt tcttcatgaa caagtggggt atcaggtatt tgaatggtgg gtgtagggtt
      181 agctttgacg atactgccat tgcttagtag caaagtcagg attaacaata gaagagcgac
      241 ttttgagaaa aacttagagt tttttgacat tgttttcatc ccgttattaa ttttttatgc
      301 tgtcacaatt ctaatccttt gtgcaatttt gcgctcgttt ttaaacacgt gtatgtttat
      361 ttgctttgga cagaacgatt ttcactcatc aaggtgccaa atacgactaa gctttttagt
      421 taagctaaaa aacaattcat tttgtaagcc gtggtcagga gaccacgagt gaactggtgg
      481 ggcttttaaa gtttggacat ggtcaggaga caatgtccat ggcaaaacct aacctttgtc
      541 gccccttcgt gataaacttt tcgtgcaatg aatccgaaat cacttattct gctggaatac
      601 cataaaatcc tgggcaaact caaagcctat gcctccttta gtggctctga agcgcttgcc
      661 acgcgtttgc gtccaaccag cagcttgccc aaagcgctca cgctgcaaac gcaaacccgc
      721 gaggcgcgct atcttttgaa tttcagcgag acggtcagct ttgccggcgt cgctgacctg
      781 gcacctttgg tgggcatggc acgcaatgcc atcgtgcttg aggcgccgga tctcatcgcc
      841 attcgcaaca cgctcatcag cgcgcgcgat gccaaacgca tcatcatcaa ccgcgcggac
      901 gaattgcccc atttggcggg aattgccgaa ggactaaccg acggcgcggg cttgattgac
      961 ctcatcaatc gcactattac cgagcgtggc gaagtgctgg atagcgccag tccggctttg
     1021 gggcaaatac ggggcaaaat caaggtcgta cacgcgcgcc tgattgaccg cctgagccgc
     1081 tacatcaacg atgccaacac catgaaactg ctccaggagc cactcatcac ccagcgcggt
     1141 gggcgttatg ttttgccact gcgcgcggag tgcaagggca gttttaagtc gattattcac
     1201 gaccaaagtg ccagtggggc gacgctgttt gtggagccag tggcggtggt tgagctgaac
     1261 aacgaatacc gtgaacttga acttgctgaa cgtgatgaag tgttgcgcgt tttgcgcgcg
     1321 ctcaccttgc gcatagccga attgcatgac agcttgctgg ccagtacacg cgccatggcg
     1381 gagctggatt taatcctgat gaaagccaaa tatgccgaag aactcgacgc ggtagagcct
     1441 gtgttgatta cctttgatga aaaacccgca ccgcatcatc cgggcagttg cattcgtttg
     1501 cgacgcgccc gtcatccgct tttggacccg gaaaaagttg tgcccattga cgttgaaatg
     1561 gacaatgaga acttcatcat cgtgctcact ggaccaaaca cgggtggcaa gaccgttacc
     1621 ctgaaaaccg ttggcctggc ggtgctgatg gcgcagtcgg gcatgcaaat cgcggcacaa
     1681 tcgggctcat gtttgagcgt ttttcgcgat gtctttgcgg atatcggcga cgaacaaagc
     1741 attgagcagt cgctttcgac tttttcggcg cacatcacgc aactggtgcg catcttaaaa
     1801 cgcgccgatt atcgttcgct cattctcttg gatgaactcg gctccggtac cgacccgcaa
     1861 gagggttctg ccctggcgcg cgcggtctta acctggctga ttgaaaaacg catcaccagc
     1921 attgtcgcca cgcattatcc tgagctgaag gcatacgccc acgtgaccaa aggcgcggtg
     1981 aacgcgtcgc tggagttcga tttgcaaacc ctcaaaccta cttatcgcct gacgataggt
     2041 ctgccgggca ggtcgaacgc cctggcgatt gctgaacgcc tgggactgcg acaagagatt
     2101 atcgacatgg cgcgccagga cattgacccc accgaacttg gcgcggatga ccttttagac
     2161 gagattcgcc ggcaacgcga tttgaccgcc gaaagttacg cgctcgccga acgtgcccgc
     2221 aaggacgcgg aaaacacgcg taatcaactc aaccggcgcc ttgacgatat cgaaaaccgc
     2281 aaggatgcca tcctggaagc cacccaggaa aaagcggaag aagagcttga agccatgaag
     2341 caggaattgc gtgagctcag gcgtattttg gcgcgtttgc aggccgcgcc ggagaagaaa
     2401 gcagaactgg cggaagtggt tgaaaaagtt gagaacattg aaaagcgcca tgaaaagaaa
     2461 gcccgccaaa taaagcgtcg cccacggccc aagtcagaaa aaggaccttt aaaagttggc
     2521 gataaggtca tcgtgcgcaa gttgggcgcc gaggcggtga ttagcgcgat agatggcgct
     2581 gatattgagg tgcaggcggg cgttttgcgc ttgcgtttgc gcactgagga tttgcgtcgc
     2641 aaggtggacg aagaaccgga agagtttgtc tctgatgaag ccaaacccag cgagcccaaa
     2701 cccagccagc ccaagccagc caggaaaggc cgcactcgct tgccaacggt ggaaatgccg
     2761 gctttggagc ttgatttgcg cggcaaacgc gtggatgatg gtttggatga attccaacgc
     2821 tggctggaac gtggctttgc ggcaggtatg ttgtttggac gcgtgattca cggacatggt
     2881 accggcgcaa tgaaagacgc ggtgcgtgct gaactgcgcc aatcgcagtt tgtcaaacgc
     2941 tgggaacccg gtggcgaaaa agaaggtggc gatggcgtaa gcgtggtctt ctttaatcac
     3001 cagacctgat tttttgaaca aaacaaaaac cccttgtgac ttatcgcaag gggttttgtt
     3061 atagatcatc tttatagaat cagcaaaaac gtttaaggct gcggttattc aaactccagc
     3121 aaaaacaggt tgccatcgcg cccactttga ttgatgctga aatagcgcgt ggtaccggtt
     3181 tcgtccacaa aagatatgcc ccggttgggg attgagccca taaagaccat gccgatcacc
     3241 aaagggcgct cggggcgcag ttcgttcatg ctgtaaagtt cttcaacgat atcaaaagaa
     3301 cactcgtcac ccacacagtc agtgagcaaa atctcaaggt atttgaagtc tttgacggtt
     3361 atatttgcca tgaaaacgac cttggccaac gagtcagaag cgtcaatgat gacctcgtca
     3421 taagtgcctg ggttggcaag caattcgtcg ctggcgtagt ctacacggat agcattgagt
     3481 aggtttgtgg tatctgtcgt gatttgccca aagccgtgtt cttctttcca tcgcggatat
     3541 tccgcgaatg ggatgagctc aacggataca ggctcgctcg ctagttcttt tcttttttcg
     3601 gcaagttctt catcacttag ctccaccgat acagccttat ccgactggca gctggtgttg
     3661 taataatttc cgatttcgct tagcgtttca tctttttcat aggtgaaaaa gtaatgttcg
     3721 caattcaggg tcaagccatc aggcgagagt ttgtaaatgc cttcaatgct atacatggcg
     3781 ccgtttgaac ctgtttgata aaacctgcca tcgccatgat aaaagtaagc atttcggtac
     3841 cagcccgtga aagtcaacgc gggttcgttg tcttgcaggg cgaaaacagc gtaggcgggg
     3901 gcgagaaggc tgactgcttg tgagtctgca gcaatttcac cgatgaataa ttctggcaga
     3961 ccgtctccgc ttaagtcggt gatggcgaag cccgtgcttt caagcgcttc atcaggggtc
     4021 ctgaaacttg cctgttctcc gatgcccacc gtgccatatt cataatcgaa gtctgtgctg
     4081 ccactgacga tgaactggta aaagtattca agggtatcaa ggtaagcccg cgccacgtca
     4141 aattcaggtt cggcgggtgt ggcggtcggt tccggctcgg attctgttgg gcctgtttca
     4201 ggcgagagat tgttatccct ctcttgggca ggtcgatcgt gggtttgcaa agcatcctgg
     4261 ttttgaccac atccagccaa agagattaat acaatcatga ctattaaaac gaatagactt
     4321 tttcttattt tcataacctt acctttataa gtcatttttc accgcctaat gataccacca
     4381 gatga
//
LOCUS       DAOSFC010000107         4384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_18041_length_4384_cov_3.868099, whole genome shotgun sequence.
ACCESSION   DAOSFC010000107 DAOSFC010000000
VERSION     DAOSFC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18041_length_4384_cov_3.868099"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PK791_03715"
     CDS             <1..383
                     /locus_tag="PK791_03715"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOH92454.1"
                     /translation="EAISVGASTAIFGLIGAQAVFIGKNRKIFGNRARQMLFRIGMIL
                     LLNFAISVVPGSSIDMWGHLGGLLAGAVFSILAAPKLEIARKDGRLILADKTAKTDIF
                     MAFLMVLLAFGVILFFAIRKAKGL"
     gene            380..961
                     /locus_tag="PK791_03720"
     CDS             380..961
                     /locus_tag="PK791_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH92455.1"
                     /translation="MKPFLLRLLSLDERLSAGLALDSPPKAVELFLKFISHSCDSWYW
                     LVGLLLVWLLAGQNGKEIAVSMGFVTGFLAITILLIKFLFRRPRPEGDWGQVYRVNDP
                     HSFPSGHAARAAAIVVVVARFLPWWVVLIVAVWAILVGYSRVALKVHYLSDVLFGFLF
                     GAIFAFGMLWVFARLMQIFPKIARFLLQGFWRR"
     gene            complement(998..2449)
                     /locus_tag="PK791_03725"
     CDS             complement(998..2449)
                     /locus_tag="PK791_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOH92456.1"
                     /translation="MTKHLDVTPDWVKNAVFYQIFPDRFAWSPKVEKPSNFEPWDSEP
                     TVHGFKGGDLMGVVEKLDYLQDLGINAIYFNPIFQSASNHRYHTHDYYQVDPLLGGNE
                     AFKLLVEEAHKRDIKIVLDGVFNHASRGFFQFNHIMEVGKDSPYVDWFHIHGYPLNAF
                     EGKPNYECWWGLPALPKLNTDNPQVRRYIFDIARYWIEQGADGWRLDVPFEIDDDEFW
                     REFRDVVKTANPEAYIVGEIPCEAQRWMAGDQFDAVMNYQITHAALGFFGGKQIDYEL
                     ASGMMGLGEAEKYTAENFAKRTEELLQIYPYEFALAQMNLLDSHDMPRFLSLVSGNVK
                     RLALAYLFILTYPGAPSIYYGDEIGLTGGKDPECRKTFPWDENEWNTDLRDQLKSYIA
                     LRNANPVLRTGAYKVIYAHDGVIAYTRKNDEKTMLVILNANDSPKTVTIPTGDAFAVG
                     TKLKSQLSDRELVVTINGIEDLELKCLSGLVLA"
     gene            2564..2655
                     /locus_tag="PK791_03730"
     tRNA            2564..2655
                     /locus_tag="PK791_03730"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2598..2600,aa:Sec,seq:tca)
     gene            2765..4108
                     /locus_tag="PK791_03735"
     CDS             2765..4108
                     /locus_tag="PK791_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH92457.1"
                     /translation="MATETSGTATPHPSKTASKTPAPTLTSTPIPTVESLLERMDGQL
                     AGVNLVLRHPWYGKAEERFNALIETFNNENGYGIKVSPVGGYGLETMADLLSNETLDA
                     NLVIAQTYDILSANENNAFVDLSEFINDPELGILDLYDESFAFNELCPLENDDFRQAI
                     AIAYQPAVLYYNTSWAKELGFDEIPLDLEIFTTQMMAGFEANTHDNDPNNNGTGGLFL
                     SKSVQSAQAWYAGFDGVFSPGASHLQFDEKALLDSYEWLKTAYAQDAHWVGAETIPYD
                     YFARRLALAVEGGLDDLAYITAAINSSSNKDEWTTMPYPSLDGHGVIAMESLGIGILE
                     SDAHKQLASWIFARYLLEQDQQAALVEVHGYWPVTGAPALIAPKYAKENPAWASAALP
                     DAHYILAPEAENWAITRRIFQDAYLRVYGLEAQYFPNILDLLKQTLIDVQGSSDD"
     gene            4101..>4384
                     /locus_tag="PK791_03740"
     CDS             4101..>4384
                     /locus_tag="PK791_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOH92458.1"
                     /translation="MTKLPKVEMWTDGACTGNPGPGGWAAILRSGKHEKVLTGGERAT
                     TNNRMEITAALEGLRALKEPCQVVIYTDSKYLMQGATEWMPNWKARGWKR"
BASE COUNT         1059 a          922 c         1142 g         1261 t
ORIGIN      
        1 cggaagctat ctcggtgggt gcgtcaacgg cgatttttgg tttgatcggc gcgcaagcgg
       61 tctttattgg caaaaaccga aaaatttttg gcaaccgcgc gcgacagatg ctttttagaa
      121 ttggtatgat tttattactc aattttgcca tctcggttgt gcctggatct tcaattgata
      181 tgtggggaca tttgggcggt ctcttggcgg gggcggtgtt ttcgatctta gccgcgccta
      241 aactggaaat agcccgcaaa gatggtcgtt taattttggc cgataaaact gccaaaacag
      301 atattttcat ggcttttttg atggtcttgt tggcgtttgg tgtaattctg ttttttgcga
      361 ttcgtaaagc aaaaggtcta tgaaaccatt tcttttgcgt ctgcttagct tagacgaaag
      421 actttccgca ggtttggctt tggacagtcc gcccaaagca gttgaattat ttttaaagtt
      481 tatcagccat agttgcgact cgtggtattg gctggtggga ttactgttgg tgtggctgct
      541 agcggggcag aacggcaaag aaattgcagt ttctatggga tttgtaacgg gttttttagc
      601 cattaccatt ttgctgatta agtttttgtt tcgtcgtcca cgtcctgaag gtgattgggg
      661 gcaggtttat cgtgttaatg atccccattc ttttccatct ggccacgccg cgcgcgctgc
      721 ggctattgtc gtggttgtgg caagattttt gccctggtgg gtggtcttaa ttgtcgcggt
      781 gtgggcaata ttggtgggat attcgcgtgt tgcgctgaaa gtgcattatc tctcagacgt
      841 cctgtttggc tttctttttg gcgcgatatt tgcgtttggc atgctttggg tattcgcccg
      901 cttaatgcaa atcttcccca aaatcgcccg atttctttta cagggatttt ggagaagatg
      961 agttttctgc aattgcaggg attgtttttt taaacgctta agctaaaacc aaaccactca
     1021 agcatttcag ttccaggtct tcaataccgt ttatggtaac aacaagttcg cggtcagaga
     1081 gttgactttt aagctttgtg cctactgcaa acgcgtcacc agtggggata gtgactgttt
     1141 ttggcgaatc attggcattg agtatgacca gcatagtctt ttcatcgttc ttgcgggtat
     1201 aggcaatcac gccatcatgc gcgtagatga ccttgtatgc gccagtgcgt aatacaggat
     1261 tggcgttgcg cagagcgatg taggatttca actggtcgcg caaatcggta ttccattcat
     1321 tttcatccca tgggaaagtc ttgcgacatt caggatcttt tccacccgtt aagccgattt
     1381 cgtcaccgta gtaaatgctg ggcgcgccag ggtaggtgag gataaacagg taggccaggg
     1441 ccaggcgttt cacgtttcca cttacaaggc taaggaagcg gggcatatcg tggctgtcga
     1501 gcaagttcat ttgggcaagc gcaaattcgt aggggtagat ttgcagtaat tcttcagtgc
     1561 gtttggcgaa attctcagca gtgtattttt cagcttcgcc aagacccatc atgccactgg
     1621 caagttcata atcaatctgc tttccaccga aaaagccaag cgcagcatgc gtgatttggt
     1681 agttcatcac ggcgtcaaac tgatcgcctg ccatccagcg ttgggcttcg catggaatct
     1741 caccgacgat gtaggcttct gggttggcgg ttttaaccac atcgcgaaat tcgcgccaga
     1801 actcatcatc gtcaatttca aagggcacgt ccagtcgcca gccatccgcg ccttgttcaa
     1861 tccagtagcg agcgatatca aaaatatatc ggcgtacctg gggattatcg gtattgagtt
     1921 taggtagagc gggcagtccc caccagcact cataattggg tttgccctca aaagcgttta
     1981 gcgggtaacc gtgaatatgg aaccagtcca cgtaggggga gtctttgcca acttccatga
     2041 tgtgattgaa ctgaaagaat ccgcggctgg catggttgaa gacaccatcc aaaacgattt
     2101 tgatgtcgcg tttgtgagct tcttcaacaa gcagtttaaa ggcttcgtta ccgcccagga
     2161 gtgggtccac ctggtagtaa tcatgggtgt gatagcggtg gttggaagcg gattggaata
     2221 tagggttgaa gtagatggca ttgatgccaa ggtcttgcaa gtagtcgagt ttttccacga
     2281 cacccatcag gtcgccacct ttaaagccgt gcacggtggg ttcgctgtcc cagggttcaa
     2341 agttgcttgg tttttcaact ttgggactcc aggcaaagcg atcaggaaaa atctgataga
     2401 atacggcatt ttttacccag tcgggcgtga catctaagtg tttcgtcatt tttacctcta
     2461 atgtgcatca aaataaagtt tacacatcaa ctatattacc ataaataatc aagaactgaa
     2521 aaattattgc atatactggt gaaattcggc tatacttatc ggtgggaatg gttaggtccc
     2581 ggtgggcttc ctggtcttca aaatcagtgt cgggttgata agatccgtgg tgggttcgac
     2641 tcccatccat tcccgccttg ttattaagga tgtgattttg acttattcac gacgttttct
     2701 actattattt ttagcatttg tttttctcct agctgcttgt aaaactcagg gagtggaatt
     2761 gcctgtggct acggaaactt caggcactgc tacgcctcat ccgtccaaaa ctgccagtaa
     2821 gacgccagca ccaacgctca cttcgacacc tataccaacc gttgaaagtt tgcttgaaag
     2881 aatggatggt caactggctg gcgttaatct ggtgttgcgg catccatggt atggcaaagc
     2941 cgaagagaga tttaatgcgc ttattgagac tttcaataat gaaaatggct atggaataaa
     3001 agtcagtcca gtgggtggct atggcttgga aactatggca gatttgttaa gtaatgaaac
     3061 tttggacgca aatctggtca tcgcgcagac gtacgatatt ctttctgcta atgaaaacaa
     3121 tgcctttgtt gatttgagtg aatttatcaa cgacccagaa ctgggaattt tggatttata
     3181 tgacgaaagc tttgcgttta atgaattgtg tccattagaa aacgatgatt ttcgacaagc
     3241 tatagctatc gcttatcagc cagctgtgct ttattacaat acaagctggg caaaagagct
     3301 tggatttgac gaaatcccct tggatttaga gatttttacc acgcaaatga tggctgggtt
     3361 tgaggctaac acacatgaca atgacccaaa caataatggc acgggtgggc ttttcctgtc
     3421 caaatctgta cagtccgccc aagcctggta tgcgggcttt gacggagtgt ttagtccggg
     3481 cgcaagtcat ttgcaatttg atgaaaaagc cttgttggat agttacgaat ggctgaaaac
     3541 tgcctacgca caagacgcgc actgggttgg tgcggaaact atcccatacg attattttgc
     3601 gcgacgcctg gctttggctg ttgaaggcgg attggacgac ttggcttata tcacagccgc
     3661 aatcaacagc agtagcaata aggatgaatg gacgactatg ccctatccga gtcttgatgg
     3721 gcatggtgtt atcgctatgg aaagcctggg tattggcatc ttagaatccg atgcgcataa
     3781 acagttggct tcatggattt tcgcgcgata tctgctggaa caagaccaac aagctgcctt
     3841 ggttgaagtc catggctatt ggccagtaac cggggcacct gctttgattg cgccaaaata
     3901 tgcgaaggaa aatcccgcat gggcgagcgc tgcgttacct gatgcgcatt atattttggc
     3961 gcctgaagcg gaaaattggg cgattacccg tcgaatcttc caggatgctt atttgcgcgt
     4021 ctatggtctt gaggcgcagt atttcccaaa tattcttgat ttgctcaaac agacgctgat
     4081 tgatgtgcaa gggagttcgg atgactaagc tgccaaaagt tgagatgtgg accgatggcg
     4141 cgtgtactgg caaccctgga ccaggtggct gggcagcaat tttgcgttcg ggcaagcatg
     4201 aaaaggtatt gactggtggt gaacgggcga cgaccaacaa tcgcatggaa atcaccgccg
     4261 cgctggaagg tttgcgcgcc ttgaaagagc cctgtcaggt cgtgatttac acagattcaa
     4321 agtatcttat gcaaggcgcg acggagtgga tgccgaactg gaaagcgcgt ggctggaaac
     4381 gtaa
//
LOCUS       DAOSFC010000108         4347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_18237_length_4347_cov_3.822227, whole genome shotgun sequence.
ACCESSION   DAOSFC010000108 DAOSFC010000000
VERSION     DAOSFC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18237_length_4347_cov_3.822227"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PK791_03745"
     CDS             complement(<1..957)
                     /locus_tag="PK791_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOH92459.1"
                     /translation="MKKETKRFFLIVVLLVFIATLFQTSSALAEDIPEVNVISPYYTE
                     VYSTLSDGTPIVGSIINGPSKPLPEYEQERLESFRTADNVQGLIQNFPSYDWNFGCSA
                     VSQAMISAYQDRTGYPNLYTGPANGGVAPLTDTSWPRWTDNYGAQYPSNPIVASRIGT
                     DGRTIKGSIEDYWVKYDSSAQDPYITGGWTQHSFGEAVGDYMRTSQSAFGNPDGATTF
                     YTYTEISDRLTCQTLENEGVQNDGTVGRKNYYQSRGYTVTECFNQRTDNSIAGGFSLS
                     QFKQEIDAGRPVMINLQGHTIVGYGYDGNTIYIRDTWSSDPDV"
     gene            complement(1027..1677)
                     /locus_tag="PK791_03750"
     CDS             complement(1027..1677)
                     /locus_tag="PK791_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH92460.1"
                     /translation="MEQKCLNCGQMNPSNQNFCTNCGNALTQPEAPASDPADGFTQVT
                     PPPAYQAPAPQQWQQAQTPPPAGEWQSPAPGAYQPGYPQYPPYQQAPNYAPSKAKAEF
                     EESKGLFISMINAHKADADSAKTDGEIARRKYPALKLFMTLMRILAWILGIGILLTGL
                     IGFFANLGSRTWWIGLIMLPVVVLVAFLTFSMCYAGIDFIRLLINTEDNTRRSANK"
     gene            2222..2485
                     /locus_tag="PK791_03755"
     CDS             2222..2485
                     /locus_tag="PK791_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92461.1"
                     /translation="METNRRVPLLLWPFYALMQLVIWILKMTGRLLGFILGIVLVVLG
                     VLLSMTLIGAIFGVPLAILGVMLAFKSLFSRQKTFSNSDNQTV"
     gene            complement(2531..3574)
                     /locus_tag="PK791_03760"
     CDS             complement(2531..3574)
                     /locus_tag="PK791_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOH92462.1"
                     /translation="MTLNTLADPENMLSYINRLPDDLELAWQLGNTLPLTDLPKPKAI
                     VICGMGGSAIGGDLFGAYMREIADVPVYSHRDYGLPAWAKGKDVLVICSSHSGNTEET
                     LDSFNTACERDCSLLTISTGGKLRERALEAGVSAWTFEHDGQPRTALAYSFGILLGLA
                     NRLSLVAGQESAIEESARVMREWRAVYSVIEEKQANPCEELAIRLLDKNVIIFGAGAM
                     EVIARRWKAQIAELAKALASFEAIPEMNHNTLAGLLFPESLVENTAAVFLSAKTDHPR
                     NQLRVEASIELFAESGVEVLEVFAKGESRLAQMWSLIQFGDYVAYYLALAYGIDPTPV
                     TILTQLKQKLAKA"
     gene            complement(3595..4239)
                     /locus_tag="PK791_03765"
     CDS             complement(3595..4239)
                     /locus_tag="PK791_03765"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOH92463.1"
                     /translation="MPDLPFGQNEPTFSHDELLQTAAMQLTYSYHETQFHLPTSTPTP
                     MFTATHTPLPTFIATIARTPTAEISPTDEKACNRAGMGVPFDVTVPDGTIFYAGESFT
                     KTWRVVNSGSCKWTRLYKLVFYSQNAMGAQYERFLEGEVLPGQAIDLSVLMTAPGKPG
                     TYQGNWMLQAPDGTLFGLGMNADTPFYVRIIVTDDPTPTPTEDLTITPEASPTP"
BASE COUNT         1121 a         1026 c         1003 g         1197 t
ORIGIN      
        1 aacgtccggg tcggaactcc aggtatcgcg aatataaatg gtattgccat cataaccata
       61 gccaacgatt gtgtgtccct ggagattaat catgactggg cgaccagcat caatttcttg
      121 tttaaactgg ctaagtgaga aacctccagc aatcgaattg tcggtgcgct gattaaaaca
      181 ctccgtaacc gtgtaaccac gactttggta ataatttttt cgtcccacag taccgtcatt
      241 ctgaacaccc tcattttcca aagtttgaca tgttaaacga tcactaattt ctgtataagt
      301 ataaaaagtt gttgcgccat caggattccc aaacgcggat tgactggtac gcatatagtc
      361 gccaacagct tcgccaaaac tgtgttgtgt ccaaccacca gtaatataag gatcctgagc
      421 gctactgtcg tacttgaccc aatagtcttc tattgagcct ttaatggtgc gtccgtcggt
      481 gccaatgcgc gaggcaacga tggggttgct gggatattga gcaccatagt tatcggtcca
      541 tcttggccag gatgtgtccg tcaatggtgc aacgccaccg tttgcagggc cagtgtacaa
      601 gttaggatat ccggttcggt cttgataagc gctaatcatc gcctggctga ccgcagaaca
      661 accaaaattc caatcgtaag atgggaagtt ttgtatcaag ccttgcacgt tgtccgcggt
      721 acggaaagat tcaagtcttt cctgttcgta ttcaggaagt ggctttgagg gtccattgat
      781 gatgcttccc acaattgggg tgccatcact gagcgtgcta taaacttctg tgtaatacgg
      841 actgattaca tttacttcgg ggatgtcttc cgccaatgcg gaactggttt gaaacagtgt
      901 agcaataaaa acaagcaaca caacaataag aaaaaatctt ttagtttctt ttttcatgac
      961 gtactctttt tctcttttat tactcaggct tgcgacttta aatctcgcaa gcccgatttt
     1021 tccttcttat ttattcgccg aacgacgggt gttgtcttcg gtgttaatta acaggcggat
     1081 aaaatcaatg cccgcgtaac acattgaaaa ggtcaaaaat gcgaccaaaa ccacgacagg
     1141 caacataatc aaaccgatcc accatgttcg actgccaaga ttggcaaaga aaccaattaa
     1201 ccctgttagc agaatgccaa taccgagaat ccacgcgaga atgcgcatca gcgtcatgaa
     1261 aagtttgagc gcgggatact tccttcgcgc aatttcgccg tctgttttag cagaatcagc
     1321 atctgcttta tgagcattaa tcatgctaat aaagagaccc tttgattctt cgaattcagc
     1381 tttagccttg ctgggggcat agttaggagc ttgctgatac ggcgggtatt gtgggtaacc
     1441 cggctggtaa gcaccaggag ctgggctttg ccactcgcca gcaggtggtg gcgtttgggc
     1501 ttgctgccac tgttgaggtg cgggagcttg atatgctgga gggggtgtga cttgcgtgaa
     1561 gccatccgca ggatctgaag ctggtgcttc aggctgagtg agtgcatttc cgcagttggt
     1621 gcagaaattt tgattgcttg ggttcatttg accgcaattg agacattttt gttccatgtt
     1681 tttctcctat agatagttat ttcttgatta gtttaaaagg gctttgaaca tatgcaacct
     1741 catggccaca gctactttat taatgcattc gacaatgatt ggttgcacaa gactttactc
     1801 cagttgaaac ataatattgg ctattatctt gtaacactaa cgttgccgga ctttttcttt
     1861 tcgtctttgg ctttacgttt ttccttgata gtcatctttg actttttacg atcttccttt
     1921 tgagctttat ctttacttgc cattaggtat atctcctgac ttcaatagtc ttagttccaa
     1981 ttataactca agcactacgg acttatagcg aatattcgct tcctaagagg tttatttaca
     2041 gaaattgaga gataatatcc ataaatcaga aaggttttac actttcataa catctctctc
     2101 atataacacc aaaatcaatt gcaaaaaaca cctaattccc tatattatct aaggtagcac
     2161 ttagattccc accctatact aaaatcagaa gtcatcgttc aaggcttcta aaggagaaac
     2221 aatggaaaca aatcgaagag tacctttact gctatggccc ttctatgccc tgatgcaatt
     2281 ggtcatctgg attttgaaga tgaccggtcg tttgctcggt tttatcctag gtatcgtgtt
     2341 ggtcgtcctc ggcgtcctgc ttagcatgac cctgattggc gcgattttcg gtgtgccttt
     2401 ggcaatcctg ggcgtgatgc tggctttcaa atcacttttt agccggcaaa aaacattttc
     2461 aaattctgac aatcaaacgg tttaactaag caaatcctgg caaggcctga ctcaactatg
     2521 gggaaaagag ctaggccttc gccagctttt gcttgagttg tgtcagaatc gtaacgggcg
     2581 tggggtcaat gccataggcc agcgccaggt aatacgccac atagtcgcca aactggatta
     2641 ggctccacat ctgcgccaat cggctttcac ccttggcaaa aacttccagc acttcaacac
     2701 cgctttcagc aaagagctca atgcttgcct ccacacgtag ttgattgcgg ggatggtccg
     2761 ttttcgcgct caaaaagact gccgcagtat tctcaaccag gctttcgggg aacaataagc
     2821 ccgccagtgt attatggttc atctcaggga tggcctcaaa cgatgccaaa gctttggcaa
     2881 gttcagcaat ctgtgctttc caacgtcggg caatcacttc catagcccct gcgccaaaga
     2941 tgatcacgtt tttatcaagc aagcggatag ccaattcttc acacggatta gcttgttttt
     3001 cttcaataac gctataaacc gcgcgccact ctcgcattac tcgcgcgctt tcttctattg
     3061 cgctttcctg tccagccacc agactcaatc ggtttgccag accaagcaat atgccaaagg
     3121 aataggccag ggcggtacgt ggttgaccgt catgttcaaa agtccaggca ctcacgcctg
     3181 cttcaagtgc cctttcgcgc aacttaccac cggtactgat ggtcagcaag gagcagtctc
     3241 tttcgcatgc ggtattgaag ctatccagcg tttcctcggt attgcccgag tgagatgagc
     3301 aaatgaccaa aacatccttc cctttcgccc acgcaggcaa tccataatcg cgatgcgaat
     3361 agacgggcac atccgcaatt tcgcgcatat aggcgccaaa caaatcacct ccaatcgctg
     3421 agccacccat gccacaaatc acaatggctt tgggttttgg caaatcagtc aatgggagcg
     3481 tattgcccag ctgccaggca agttcgagat catcgggcaa gcgattaatg taagaaagca
     3541 tgttttcggg gtcagcgagt gtgttaaggg tcatagtatc atccttttag tgaattatgg
     3601 tgtcgggctt gcttctggtg taatggtcaa gtcctcagtt ggggttggcg tcgggtcatc
     3661 ggtgacgatg atgcgcacgt aaaaaggcgt atcggcgttc atacccaagc cgaacaaggt
     3721 gccatcgggt gcctggagca tccaattgcc ctgataggtg ccgggtttgc ctggcgcggt
     3781 catcaaaacg ctaaggtcga tagcctgacc aggcaagact tcgccttcaa gaaagcgttc
     3841 gtactgcgcg cccatcgcgt tttgcgagta aaaaaccagt ttataaagtc gggtccattt
     3901 acaggaaccc gaattgacca cgcgccaggt tttggtgaaa gattcaccag cataaaaaat
     3961 ggtgccatct ggcacagtca catcaaacgg cacgcccatt cccgcacggt tacacgcttt
     4021 ttcgtctgtc gggctgattt cagccgttgg tgtacgggca atcgtggcga taaatgtagg
     4081 caacggggta tgcgtggcag tgaacatcgg tgtgggcgtg gatgtgggca gatgaaactg
     4141 tgtttcatga tatgaataag taagctgcat ggctgcggtt tgcaagagtt catcatggct
     4201 gaaagttggc tcgttttgcc caaatggcag atctggcact ttgggtttac aagccaccag
     4261 cattgatagc gataccaaga caaagaacaa aaccaaacca caaaaacgca aggttttcat
     4321 gctctaaatt gtacccgaga atggcaa
//
LOCUS       DAOSFC010000109         4311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_18444_length_4311_cov_5.730733, whole genome shotgun sequence.
ACCESSION   DAOSFC010000109 DAOSFC010000000
VERSION     DAOSFC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18444_length_4311_cov_5.730733"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1159)
                     /locus_tag="PK791_03770"
     CDS             complement(59..1159)
                     /locus_tag="PK791_03770"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH92464.1"
                     /translation="MTEKYEGTPQERKKAVNPHLAGFIAWLVAIVLCAGIVIFAFWQV
                     GVAQSMQKYQAVRSYETPEVHEEIVAAMPPFEPQAVDAGFFRKPLTETDLNTEYRTEP
                     IEYTVSSGDSVWGISERYGVSVESILYANFNILQDKSDNLKPGQVLTIPPTDGILHKW
                     TSRDTIESVASKYGANVDDILLFIGNNLDLSKPEIKPGTIVMVPGGNRMLTEVSHAAV
                     VVDDEGNRRSGFSGPGACRIIGHGYVGTGYFIWPSAVRYISGNNYGPGHRAIDIGAGL
                     GSQLFASDSGIVVYAGWLDGGYGNFVVIDHGNGFQTLYEHLENIYVRCGDNVLQGAVI
                     GTAGSTGNSTGPHLHFEIRYGGTQVNPWDYLP"
     gene            complement(1177..2448)
                     /gene="rho"
                     /locus_tag="PK791_03775"
     CDS             complement(1177..2448)
                     /gene="rho"
                     /locus_tag="PK791_03775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOH92465.1"
                     /translation="MDVFDLEKMPVKQLRKVATDLNVEGVNRLKREGLIFQIARRMGE
                     EEGVEVRGGVLEIMNEGIGFLRTNYRIGADDVYVSQAQLRRYDLRPGDLVIGRVRPPR
                     ESDRHHGLLKVESVNGLTPDEARRRPRFENLTPIFPNKLLNLEYSSSNLATRMINLVA
                     PIGGGQRAMIVSPPKAGKTSILKEIANAIAVNHPEVRQIMSLIGERPEEVTDMDRSVE
                     AEVIASTFDEHTSSHVRMAEVSLDRAKRLVEIGYDVVILMDSLTRLARAYNMVVTPSG
                     RTLSGGMDPSALYPPKRLYGAARNLEEGGSLTIIATAIVDTGSRLDDVVYEEFKGTGN
                     MEMHLSRRLQERRIFPAIDIEKSSTRREELLLDPETLQRVWLMRRMYLQMVANPPQGA
                     GMDTAVATEAILQRMEKTKTNAEFLETLTDM"
     gene            2793..4232
                     /locus_tag="PK791_03780"
     CDS             2793..4232
                     /locus_tag="PK791_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH92466.1"
                     /translation="MILIACFLAISQLTRYSRVRANFPTGLKIAGIPVGGLNYELASD
                     RLVKVYMSPIELTYGDQRIQVRPATLGYELKLQNMLAVADKQRTSESFWSGFWKYLWN
                     QPITSHDVALQAEVDENRVRAYLETEIASRYDEFAEPPMPVPGESYFSPGKKGTAINY
                     STSIERIIQALNSANNRSVRLDVVESQAAKPGFELLDYMLRDIIKQSAFDGWVELYMK
                     DLQSGQVLHFNYTKVGEEELPVNIAYSAWSTIKIPALLSAFKYLEEPYDPAILTKIEE
                     MVEQSDNESTDFVGKNVIERNLGPLRVSEDMQTLGLENTFWAGYFALGSPLLQDFKTP
                     ANQDTSYDTDPDRYAQTTPLDLGLLLEEIYYCARDYGGAIPLAFEGEITHYECQLMVE
                     YMAKNRIAVFLQGGVPPETQVAHKHGWAVEHKDGYMHTMGDAGIFYTPGGDYIVSIFI
                     FHPVQAIFDVVNPMVSKLSGAVYNYFNLE"
BASE COUNT         1157 a         1083 c          909 g         1162 t
ORIGIN      
        1 taggagtgcc ttgctctatc cattgagcca ctggggcaag ttgctgaagt atatcacttt
       61 agggaagata atcccaaggg ttcacctgcg ttccaccgta gcgaatttca aaatgcaagt
      121 ggggtcctgt agaattccct gttgagccag ctgttccaat caccgcgcct tgaagcacat
      181 tatcgccaca tctcacataa atattctcaa gatgttcata cagtgtctga aaaccattgc
      241 catggtcaat aaccacaaaa ttaccatagc caccatcaag ccagccggca taaaccacga
      301 taccactatc ggatgcgaaa agttgtgaac caagaccagc gccgatatca attgcccgat
      361 gaccaggccc atagttatta cctgaaatat atcgaacagc agatggccaa ataaaatagc
      421 ccgtaccaac ataaccatgc ccgataattc tacaagcacc cggcccggaa aaaccactgc
      481 gccggttacc ttcatcatca accacaactg cggcatggct gacttcagta agcatccggt
      541 ttccacccgg taccatcaca attgttcctg gttttatttc tggcttgctt agatcaaggt
      601 tgttaccgat aaacagaaga atatcgtcaa cattcgcgcc atattttgac gcaactgatt
      661 caatcgtgtc gcgactggtc catttatgta aaataccgtc tgtcggtgga atcgttagca
      721 cttgaccagg tttgagatta tccgatttat cctgcaaaat attgaagttg gcataaagga
      781 tggattccac actcacgcca tatcgttcag atatacccca tactgaatca ccactggaaa
      841 cagtgtattc gatgggttcc gtgcgatatt ctgtattcag atctgtttct gtaagcggtt
      901 ttctaaaaaa gccagcatcc actgcctgtg gttcaaacgg gggcatggcg gccacgattt
      961 cttcgtgaac ttccggagtt tcgtaagatc ttaccgcttg atacttttgc atggattgtg
     1021 cgacaccaac ctgccaaaac gcgaagataa caatccccgc gcaaagcaca atcgccacca
     1081 accaggcaat aaaacctgcc aggtgtggat taacagcttt ctttctctct tgtggagttc
     1141 cttcgtattt ttcagtcata ccctgattat actagattac atatcagtca atgtttccag
     1201 gaattcagcg ttcgttttgg tcttttccat tctttgcaaa atggcttcag tcgccacggc
     1261 agtgtccatg ccagcgcctt gtggcgggtt ggctaccatt tgcagataca tgcgccgcat
     1321 aagccataca cgttgtaagg tttccgggtc aagcaaaagt tcttcacgac gggttgatga
     1381 tttctcaata tcaatagcgg ggaagatgcg tctttcttgc aatcgccttg aaagatgcat
     1441 ttccatatta cccgtaccct tgaattcctc ataaacgacg tcatccaggc gcgaaccagt
     1501 atcaactatt gccgtagcaa taattgtcag gctgccacct tcttcaagat tccgtgccgc
     1561 tccataaagg cgttttggcg ggtaaagcgc ggagggatcc ataccacctg aaagagtgcg
     1621 gccacttggg gtgacaacca tattgtaggc gcgcgccagg cgtgtgagag aatccatcaa
     1681 gataaccaca tcgtagccaa tttccaccaa acgttttgct cggtcaagcg aaacttctgc
     1741 catacgcaca tgcgaagaag tatgttcatc aaatgtactg gcaatcactt ccgcttcaac
     1801 cgaacggtcc atatcggtaa cctcttccgg acgttcacca atcaaggaca taatctggcg
     1861 cacttctggg tgattgacgg caatcgcgtt agcaatttct ttcagaatac tggttttacc
     1921 cgctttgggt ggcgaaacaa tcatcgcgcg ttgcccgcca ccgatcggtg caacaaggtt
     1981 gatcattctt gtggcaaggt tgctggatga atattctagg ttaagcaatt tgtttgggaa
     2041 aataggagtg agattttcaa atcgtggtcg tcgtctggct tcatcaggtg ttaaaccatt
     2101 gaccgattct actttcagta aaccatggtg tcggtcagat tctcttggcg gacgcactct
     2161 gcctattacc aaatcgccag gacgtaaatc gtaacgacgc aactgggcct gcgaaacata
     2221 cacatcatcc gcgccgatac ggtaattcgt ccgcaaaaag ccgatgcctt cgttcataat
     2281 ctccagcaca ccaccacgga cttcaacgcc ttcttcttcg cccattcttc gcgcgatttg
     2341 gaagatcagc ccttcacgtt tcaagcgatt aacaccttcc acgttcaagt ctgtagccac
     2401 tttgcgcaac tgtttgactg gcatcttttc taaatcaaaa acatccattt ttttaccttt
     2461 cgttgaaacc ttccccttga cactttccat caagcgtcgc gatacaacat gtttcagtta
     2521 ttaatgtaaa accatttgta ataaggggaa cagcctatcg gctgatttgt aaacaataat
     2581 tctttatcgt gatttcacta aaattataac atcatctaaa atctgtgcaa gtttttttgc
     2641 aagcgttcaa atcaggctgg ttttcaatta ttaaaccgat gatttacaca ttttgcgtca
     2701 atactaagtg ctaacaaaat catcagccac aatccgatta taattgaacc atgcgcacga
     2761 aaaggtctct gattcaatgg atagcggctg gtatgatact catcgcctgt tttttggcga
     2821 tttctcaact cacccgctac tctcgggtcc gcgctaactt tccaaccgga ctaaaaattg
     2881 ccggcattcc tgttggtggt ttgaattacg aactggcttc agaccgcctg gtaaaggtat
     2941 acatgtcgcc cattgaactg acttatggtg accaacgcat tcaagtgcgc ccagcaacct
     3001 tgggctacga gctaaaactg caaaatatgc tggctgtagc agataaacaa cgtaccagtg
     3061 aatctttttg gtcaggtttt tggaaatacc tttggaatca acccataacg tctcacgatg
     3121 tcgccctaca ggcagaagtg gacgaaaacc gtgtgcgtgc ctatttggaa actgaaattg
     3181 cctcccgcta cgacgaattt gcagaaccgc ccatgcctgt tcctggtgaa agttactttt
     3241 ctcccggtaa aaaaggcacc gcgattaact atagcacgtc tattgagcgc atcattcagg
     3301 ccctgaattc agcgaacaat cgcagcgtta gacttgacgt cgttgaaagc caggcagcaa
     3361 agcccggctt tgaacttctc gattacatgc tgcgcgacat catcaagcaa tccgcttttg
     3421 atggctgggt tgaactgtac atgaaagatt tgcaatctgg ccaggttttg cacttcaact
     3481 acaccaaggt tggcgaagaa gaattgcccg tcaacattgc atattcggca tggagtacca
     3541 tcaaaattcc agctttgcta agcgcgttca aatatcttga agaaccttac gacccagcca
     3601 ttctcaccaa aatcgaagag atggtcgaac aaagcgacaa cgaatcaacc gattttgtcg
     3661 gaaagaatgt catcgaacgc aacttaggcc ccttgcgtgt cagcgaagat atgcaaaccc
     3721 tgggtcttga aaatactttt tgggcaggct atttcgctct gggttcgccc ttgttacagg
     3781 atttcaaaac gccggcaaat caagatacca gttacgacac cgacccggac cgttacgccc
     3841 aaaccacgcc cttggattta ggtttgcttt tggaagaaat ttactactgc gcccgggatt
     3901 atggcggtgc cattcccctg gcttttgaag gggaaataac gcactacgaa tgtcagctta
     3961 tggtcgaata tatggctaag aaccgcattg ccgtcttttt gcaaggtggc gttccaccgg
     4021 aaacacaggt tgcccataaa cacggctggg cggttgaaca taaagatggt tatatgcaca
     4081 ccatgggcga tgctggcatc ttctacacac ctggcgggga ctatattgtc tcaatattta
     4141 tcttccaccc cgttcaggcg attttcgacg tcgtcaatcc catggtaagc aaactatccg
     4201 gtgctgttta caattatttt aatcttgaat aagcctaaat ttcatgtata attatgcaac
     4261 ttgcccgcgt agctcaatgg atagagtacc tgacttcgaa tcagatggtt g
//
LOCUS       DAOSFC010000110         4282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_18605_length_4282_cov_6.444050, whole genome shotgun sequence.
ACCESSION   DAOSFC010000110 DAOSFC010000000
VERSION     DAOSFC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18605_length_4282_cov_6.444050"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..981
                     /locus_tag="PK791_03785"
     CDS             175..981
                     /locus_tag="PK791_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92467.1"
                     /translation="MLTSNDLLKFDLLDHPFERFLDTRFFYPGISEQRKVLEVAYDFI
                     ADQKDPSKNLGVIAGPSGGGKSILAMKLAQSRLRFDGQGELHGLYMNTNTLTEPRHFL
                     MAIVDLLGLRGSRSNADRIESILDYLGNSPEQVLLVLDGPPVDIEYMDQLLSWSIENN
                     KKIKTLIFLQDLNKTTSNIGSLNQYLGLYFPFRNADTSEIAAMLFARMQAAGHPNPLS
                     LIPETTLYEIAENSRGTLSEALRLASIALEELISVQREDQMLKKLPLFSK"
     gene            1089..3257
                     /locus_tag="PK791_03790"
     CDS             1089..3257
                     /locus_tag="PK791_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92468.1"
                     /translation="MKDWRALQDRLATVADDKLRVLLDSCEVQFDGQYQINASYSDYA
                     RLCQYYPLLETVVHDNLGKHVRLNLKQSVPMGTDDDDSIIGDLALDFELGANSVSDAI
                     LRPESIAAVPSYTLRFVPYVGANAVLIATALRQAFYRASRETGADQLYPKYGDSVKID
                     VRGLLKSLGGAISRASFFRVFKNGDMDWFVTRGEPKHRVKDGQVQREANTYHYRGILL
                     TPGDACDLYDWLIHNNLSEQPIAVLSHLLGTRRDEILTFPFRVPDAEKDIAFDQPAST
                     HEIIEKALGNQKLDPTLAGLADRVAMHLVRPESFLAVPWYWFHKVLPTLGTDLGVLYL
                     MCKNCCYVDWAHGKDRNSFWVPSGLTSLQAWIRNDSLPKQIPHAEQSQRGRPRKENIN
                     QDSAYTRNWRETKRQLASEYLCRIETRQSETGTDWKLQVNDVQLTAADESLKQAIYGF
                     IYAPPKQIAGLPMQELMQSPEFQQSLLANAKANPTQLCHFETLAKEGICNSDTLDHAL
                     ICHFDTFVDGLNYYFETLVGAGICQFETILNILFKLKYTSFFNQDTENQPHTSATDDD
                     EDDFCNTDILFQVVEKELMLSDWDYQSLFSVLDKKYQEQIQLLKLEKITVAWAVQACF
                     ILNINQPWSLAIRRAIDLRKAPGGPAMRMAELPMYKFKELIESTYHRMEANYFNPLQL
                     TMSGGQDLLDLLELFDNVEDQKRALRRMRDTFGLFSPDND"
     gene            complement(3254..3556)
                     /locus_tag="PK791_03795"
     CDS             complement(3254..3556)
                     /locus_tag="PK791_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92469.1"
                     /translation="MDQKQSKFEISAQEESVEPRADASTHLNGILPSVKQKATNQTKH
                     DPITPNQRAHPGVLLTLLVIVIVLIALLILGIHLINKNLEALAFLSPVLISHIGCI"
     gene            3885..>4282
                     /locus_tag="PK791_03800"
     CDS             3885..>4282
                     /locus_tag="PK791_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOH92470.1"
                     /translation="MSQFEIKGQYNTAVVFARDREEKAIEQIRTLVSQPFVAGKKVRI
                     MPDYHFGAGCTIGFTADLGDIVVPNLVGVDIGCGILVTELGYDQPDLAALDNYIHHNI
                     PSGFGSYKKPVVEFDEINKLKSAHAVPVKKA"
BASE COUNT         1194 a          903 c          935 g         1250 t
ORIGIN      
        1 gtgattccaa tcttgttttc attttacctt agttttggta aaatacaagt cagagaatgt
       61 ttgtgattcg tttttttcgt tcacttacta caactagcga gttttatttt ggtacttgac
      121 ttaactctta cacaatgcta atctattatc aagatttcgc tggaaggtga ttttatgtta
      181 actagcaacg acctactcaa gtttgactta ctcgaccacc cctttgaacg ttttttggat
      241 acacggtttt tttaccccgg tatcagtgaa caacgcaagg ttttagaagt cgcttatgac
      301 ttcatcgctg accaaaaaga cccttcaaaa aaccttggtg tcatcgctgg acccagtggc
      361 ggagggaaaa gcattttggc gatgaagttg gctcaatcca gactgcgctt tgatggtcaa
      421 ggcgagttac atggattata catgaacact aacacgctta ctgaaccgcg ccactttttg
      481 atggcaattg ttgacctgct tggtctgcgt ggttcccgtt ccaacgctga ccgcattgaa
      541 agtattctgg actatttggg aaactcgccg gaacaagtat tgcttgtcct ggatggtccc
      601 cctgttgata tcgagtatat ggatcagctt ttgagttggt caatagagaa taacaagaaa
      661 attaagacgc tcattttcct gcaagatctg aataaaacga ccagtaatat tggttcactt
      721 aaccaatatt taggtttgta tttccccttt agaaatgctg acacaagtga aatcgcagcc
      781 atgcttttcg cgcgtatgca agctgccggg catcctaatc cgctttcctt aatacccgaa
      841 acaacgctat atgaaattgc tgaaaactcc cgtgggacgc tctctgaagc cttgcgattg
      901 gcttctattg cccttgaaga gctcatttca gttcaaagag aagatcaaat gttaaagaaa
      961 cttccacttt ttagcaaata ggggtgtcaa tttccacttt tgacacttgc tttaacagaa
     1021 cttattttct attatatagg catacctaaa ggagccattc taaatagaag attgaaatga
     1081 aaaaagaaat gaaagattgg agagcactgc aagataggct cgctactgtt gcggatgaca
     1141 agttgcgagt attgcttgat tcttgcgaag tccaatttga cgggcaatat caaatcaacg
     1201 ccagttatag cgattatgcc aggctttgcc aatactatcc cttgcttgaa acagttgtac
     1261 atgacaatct cggcaaacat gttcggctca atctaaaaca aagtgtgccc atgggcacag
     1321 atgacgatga cagcataatt ggcgaccttg cgcttgattt tgaacttggc gcgaattcgg
     1381 ttagcgatgc cattttacgc ccagagtcca tcgctgcagt gccatcatat accttgcgtt
     1441 tcgttcccta cgtgggcgcg aacgcggtgc ttatcgctac agcattgcgc caggcttttt
     1501 atcgtgcttc gcgcgaaact ggcgctgacc agctttatcc caaatatggc gatagcgtca
     1561 aaattgatgt gcgtggatta ctcaaaagtt tgggtggtgc gattagccgt gccagtttct
     1621 tccgcgtttt caaaaatggc gatatggact ggttcgtgac gcgtggtgaa ccaaagcatc
     1681 gggtaaaaga tggacaagta caacgcgaag ctaatactta tcattatcgt ggcattctct
     1741 taacacctgg cgatgcctgt gacctttatg actggttaat ccataataat ctaagcgaac
     1801 aacccatagc cgtgcttagc cacttacttg gtacccgccg cgatgagata ttgacatttc
     1861 ctttccgcgt tcctgatgca gaaaaagaca tcgcctttga tcaacctgct tcaacgcatg
     1921 aaatcatcga aaaagccctt ggtaatcaaa agctggatcc cacactcgcg ggtcttgctg
     1981 atagggtggc gatgcatctg gtgaggcctg aaagtttcct tgctgttcct tggtattggt
     2041 tccataaagt cttgccaacc ttgggcactg atttaggtgt gctttatcta atgtgcaaaa
     2101 actgctgtta tgtggactgg gcacacggta aagaccgcaa tagcttttgg gtgcctagtg
     2161 gattaacaag cctgcaggca tggattcgca acgatagtct gcccaaacag attccccatg
     2221 cggaacaaag ccaacgcggt cgaccgcgta aagaaaatat caatcaggat tctgcctata
     2281 cccgcaactg gcgcgaaacc aagcgtcagc ttgccagtga atatctgtgt cgcattgaaa
     2341 cccgacagtc agaaacaggt accgattgga aattgcaggt aaacgatgtc caactaaccg
     2401 cagcagacga aagccttaaa caagccattt atggtttcat ttacgccccg cccaagcaaa
     2461 ttgcagggtt accgatgcaa gaattgatgc aatctccaga gttccagcag tctcttttag
     2521 ccaacgccaa ggcaaaccca acacaattat gtcattttga gactctcgca aaagagggaa
     2581 tatgtaatag tgatacgctt gaccatgccc taatatgtca ttttgatact ttcgtggatg
     2641 gtctaaatta ttattttgag actctcgtcg gtgctggaat atgtcaattt gagacgatat
     2701 taaatatact ctttaaactt aaatacactt ctttttttaa tcaagataca gaaaaccaac
     2761 cacatacctc tgccacagat gatgacgaag atgatttctg caatactgac attctttttc
     2821 aggtggtgga aaaagaatta atgctttctg actgggatta tcaatcactt ttttctgttc
     2881 tagataaaaa atatcaggaa caaatccagc ttttgaaact tgaaaaaata accgttgcct
     2941 gggctgtgca ggcatgtttt atccttaaca tcaatcagcc ctggagtttg gctatccggc
     3001 gcgccattga tttgcgcaaa gctccaggcg gacctgctat gcggatggcg gaactgccga
     3061 tgtataaatt caaagaactg attgaaagca cctatcaccg tatggaagca aactacttca
     3121 atccgctcca gctgacaatg tccggtggcc aggatttact ggatttactc gagttgtttg
     3181 acaacgtgga agaccaaaaa cgtgccttgc gtcgtatgcg cgacacgttt ggtttgtttt
     3241 cacccgacaa tgactagata cagcctatgt ggctaataag gacgggagac aaaaaggcca
     3301 gcgcttctaa gtttttgtta atcaaatgaa tacccaggat taacagggca atcaacacga
     3361 taacaatgac cagcaaggta agcaagacac caggatgcgc cctttgattt ggcgttattg
     3421 ggtcgtgttt cgtttggttt gttgcttttt gttttaccga aggcaagatg ccgtttaagt
     3481 gggttgatgc atcagctctt ggttctactg attcctcttg ggctgaaatc tcaaatttgc
     3541 tttgtttttg gtccatattt catttccttt aatctgttgc taattgtacc ggcattttgt
     3601 taatgtattc catagttgct tggctttttg taattatcaa attatgaaaa tctttttgtt
     3661 taagaatatg tatctgtgga tgaagtttat gttcctgttg tgtaatatct aatccgaccc
     3721 taaattaatg cgcgtagcca cttattaagt gaggtaaagt gagagaaaaa taaatgaaga
     3781 gtgacgggtg ttgaaccaca gtttggcttg aaaagcataa acaatggagt ttcgtataag
     3841 ttcatagaac ggagtttcta caatgctggt acaatgtaat gactatgagt caattcgaaa
     3901 ttaaaggaca atataacact gcagtagtat tcgcacgtga ccgcgaagaa aaagcgattg
     3961 aacaaattcg caccttagtt tcgcaacctt ttgtggcagg taaaaaggta cgcattatgc
     4021 ctgactacca ctttggcgca ggttgcacga ttggttttac cgctgacttg ggtgatatcg
     4081 tggtgcctaa tttggttggt gtggatattg gttgtggaat cctggttact gaacttggtt
     4141 atgaccaacc tgatttagca gcgcttgaca attacattca tcacaatatc cccagtggat
     4201 ttggcagcta caaaaaaccc gtcgtggaat ttgatgagat taataagctc aaaagcgcgc
     4261 acgctgtacc ggtcaaaaaa gc
//
LOCUS       DAOSFC010000111         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_18830_length_4245_cov_4.885203, whole genome shotgun sequence.
ACCESSION   DAOSFC010000111 DAOSFC010000000
VERSION     DAOSFC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18830_length_4245_cov_4.885203"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PK791_03805"
     CDS             <1..1008
                     /locus_tag="PK791_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH92471.1"
                     /translation="KAMPGVKQVVIKDYLRGGANLSYDKMEGWGNFQALNITDLKEMG
                     LEASQGTTIAEKGQMVLGSQYKNNFYDPNPKPGEEPRMIDDLFGKTIDVVFIKWDNEG
                     NEAKRTVKFEVVGILKESRSEADWSMYMNYDELNRWNEWFAGKRIDYNKEGYQNVSVR
                     AESPEVVVELADKITEMGFQAYTPQEAVEGINSFFAIMQIVFGGIGAIALLVAAIGIA
                     NTMTMAILERTREIGIMKAIGATNNNILAIFLGEAAGIGFLGGVGGVLLGWAMCAVVN
                     ALGSSLLASGSGGTQLNAVIPLWLPIFSIAFATVVGLISGLFPSLNAATMVPVDALKY
                     E"
     gene            complement(1074..1844)
                     /locus_tag="PK791_03810"
     CDS             complement(1074..1844)
                     /locus_tag="PK791_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92472.1"
                     /translation="MTQCNHGLMQAFKLVKKVQENEKTTTMWLDGNLENATPGQYVMA
                     WLPDVGEKPFSLHSATPLALTICAVGPVSRAITALNSGDTLWIRGPLGKGFSLTGKKH
                     LLVGGGYGAAPLSYLADEALKRGDQVVTCLGAASAKDLMLIDELTRLGATVHVTTDDG
                     SFGTQGLVTAVVLEQIETDKPDCLYACGPTGMLMALWDICQAHKIPVQLSFEALTRCG
                     LGLCGACELDETLCARLGLPSGWLVCHDGPVARLNLQN"
     gene            complement(1831..2772)
                     /locus_tag="PK791_03815"
     CDS             complement(1831..2772)
                     /locus_tag="PK791_03815"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOH92473.1"
                     /translation="MANLAVDFLGIKLNNPLVLASGVLGTSPAVMERCAREGVGAVTS
                     KSAGTEARDGHANPVALAWQGGVINAIGLTNPGCHAELPILIETKKRLKPLGVPLFAS
                     IFAPRLEDFGEVARVIAEAEPDLIEVNISCPNVASDFGTPFSATAKSAAEVTREVRKA
                     VNLPISVKLAPNVYNLGEIARAVVAEGADAITAINTVPGMLIDAYAARPVLKNTTGGL
                     SGPTIKPVALRAVWEISQVVDVPIIGTGGISSGIDAAEMIMAGAALVGVGSALVNGGP
                     AVFGKINTELQEFMRSQGYQDLESMRGLVHRRANDTV"
     gene            complement(2765..4174)
                     /gene="pyrF"
                     /locus_tag="PK791_03820"
     CDS             complement(2765..4174)
                     /gene="pyrF"
                     /locus_tag="PK791_03820"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOH92474.1"
                     /translation="MSFIEKLTQTIEKNQSLLCVGLDPNEKNLPEGDNIQEKLLLWAG
                     NIISQTKGLVCCYKPNMAFFEQFGLEGLEALQVICRELIPADVPVLLDAKRGDIGSTA
                     EAYARACFDIYGADAVTLSPYLGYDAIKPFTDRADKAVFVLCQTSNPSAKEIQQHGIP
                     TLFEHVAKQSQHWGAAEQVGLVVGATQPEALKRVRELCPDAWILAPGVGAQGGNLRLA
                     LENGLRADGLGMIVPVSRSVMNAEDPKAAAEALRDEINRIRIDVQIKLLDAPKVSRFA
                     ELIEGLYDTGSVKFGEFTLASGKNSPVYLDLRRLVSFPWLMKLAVEAYAEQMHNTPCD
                     LIAGVPYAALPIASVLASQAGIPMIYPRKEVKTHGTGQLVEGVFEAGQSVTVIEDVIT
                     SGGSLITAVESLRSLGLKVEHAIVLVDREQGGMEKLAQAGITAHPALKFSEILEHLLA
                     TKKIDKEQYTIVKDYLSHG"
BASE COUNT         1085 a         1110 c         1031 g         1019 t
ORIGIN      
        1 aaagccatgc caggtgtaaa gcaagtggtc atcaaagatt acttacgggg cggagctaat
       61 cttagttacg ataaaatgga aggttgggga aacttccagg cattaaatat caccgatcta
      121 aaagagatgg gtctggaagc cagccagggc acaaccatag cagaaaaagg acagatggtt
      181 cttggttcgc aatacaaaaa caacttttat gaccccaacc ccaaaccagg tgaagaacca
      241 agaatgattg atgacctgtt tggtaaaacc attgatgtgg tttttattaa atgggacaat
      301 gaaggtaacg aggccaaacg cacagtcaaa ttcgaggttg ttggcattct aaaggaatca
      361 cgttctgaag ccgactggag tatgtacatg aattatgacg aactcaatcg ctggaacgaa
      421 tggttcgctg gtaaacgcat tgactacaat aaagagggct atcaaaacgt ctccgtgcgc
      481 gcggaaagtc ccgaagtggt tgtggaactc gccgataaaa tcactgagat gggttttcag
      541 gcttacaccc cacaggaagc cgtggaaggt atcaatagtt tcttcgcgat tatgcaaatt
      601 gtcttcggtg gcattggcgc gattgcgctt ttggttgccg cgattggcat cgccaacacc
      661 atgaccatgg cgattttgga acgcacccgc gaaattggta tcatgaaagc catcggcgcg
      721 actaacaaca atattctagc catcttcctg ggcgaagcgg caggtattgg ctttttaggc
      781 ggtgttggag gcgtgctttt gggctgggcg atgtgcgcgg tggttaacgc gctgggcagc
      841 agcctgttgg cttcgggcag tggcgggact cagctgaacg cggtcattcc cttgtggctg
      901 ccgattttca gcattgcctt tgccacggtg gttggtttaa ttagcggctt gttcccatca
      961 ctaaacgccg caaccatggt gcctgttgac gcgttgaagt atgaataatt aatgttttaa
     1021 gttgctaaaa agtccggttt cccggacttt tttgttaatt tacttagcaa gttttaattt
     1081 tgcagattta acctggccac agggccatca tgacaaacca accagccaga cggcagaccc
     1141 agacgcgcgc ataaggtttc atccaactca cacgctccac acaaacccaa accacaccgc
     1201 gttaacgcct caaatgacag ttgcacgggt attttgtgcg cctggcatat atcccacaac
     1261 gccatcagca ttcccgttgg accgcaggca tacaaacaat cgggtttgtc ggtttcaatc
     1321 tgttcgagaa ccaccgccgt taccaagccc tgggtaccaa acgatccatc atcggtcgtt
     1381 acatgaaccg tcgcgcccag cctggttagc tcgtcaatca gcatcaaatc ttttgctgat
     1441 gccgcgccca gacaagtgac tacctggtca ccacgcttta aagcttcatc ggccaggtaa
     1501 ctcaatggcg cagcgccata gcctcctccc accagtaaat gcttttttcc agtaagcgaa
     1561 aagcccttgc caagtgggcc tctaatccac agggtatcgc cggagttcaa tgcggtaatc
     1621 gcgcgcgaaa ctggacccac cgcgcaaatc gtcagggcga gtggcgtggc tgagtgtaag
     1681 ctaaagggct tttcacccac atcagggagc catgccatca catattgccc cggggtggca
     1741 ttttccaggt tgccatccag ccacatggtt gttgtcttct cattttcctg aactttcttt
     1801 acaagtttaa aagcctgcat cagaccatga ttacactgtg tcattcgccc tccgatggac
     1861 caaaccacgc atagattcca aatcctgata accctgcgaa cgcataaatt cctgcaactc
     1921 ggtatttatt ttgccaaata ccgctggtcc gccattcaca agagcggaac ccacaccgac
     1981 caaagccgcg ccagccataa tcatctcagc cgcgtcaata ccagatgaaa tgccacccgt
     2041 gccgattatg ggtacatcca ccacctggga gatttcccag accgcgcgta aagcgactgg
     2101 tttgatggtg ggtccactta aaccaccggt tgtgtttttg aggacgggcc tggcagcgta
     2161 ggcatcaatg agcattcctg gcacagtgtt gatggctgta atggcatcgg caccttccgc
     2221 gaccaccgcg cgggcaattt cgcccaggtt gtagacgttg ggcgcgagtt tgacactaat
     2281 aggaagattc actgccttgc gtacttcgcg ggtgacttca gcggcacttt tggcagtcgc
     2341 cgagaatggc gtgccaaaat cagatgcgac attagggcat gaaatgttga cttcaatcaa
     2401 atccggttcc gcttcggcaa ttacacgggc gacttcgcca aaatcttcaa gccttggggc
     2461 gaaaatggaa gcgaacaaag gcacacccaa gggtttaagc cgttttttgg tttcaatcaa
     2521 gataggaagt tcagcatggc atcccgggtt ggtaagcccg atggcgttaa tcaccccacc
     2581 ctgccaggcg agagcgaccg ggttggcatg gccatcgcgg gcttcggtgc cagcactttt
     2641 gctggtaacc gcgccaacgc cttcgcgcgc gcagcgttcc atcaccgcag ggctggtgcc
     2701 caaaacgccc gaggcaagta ccaaaggatt attgagctta atccctaaaa agtcaactgc
     2761 cagattagcc atggctgagg taatccttca cgatggtgta ttgttcttta tcaattttct
     2821 ttgtcgctaa caaatgctcc agaatttcgc tgaatttgag agctgggtgg gcggtgatgc
     2881 cagcctgagc aagtttttcc atgccacctt gctcacggtc aaccaatacg atggcgtgtt
     2941 ccaccttgag accaagtgaa cgcaggcttt caaccgcggt aatcaggctg ccaccgctgg
     3001 taatcacgtc ttcaataacc gtcactgact gaccagcttc aaaaacgcct tccaccagtt
     3061 gaccagtgcc atgtgtttta acttccttac gtgggtaaat cattggtata ccggcttggc
     3121 tcgccagaac agatgcgatt ggcagtgcgg cataaggcac gccagcaatc aaatcacaag
     3181 gtgtgttatg catttgctca gcataagcct caactgccag tttcatcaac cacgggaagc
     3241 tcaccaagcg acgtaagtcc agataaaccg gcgaattctt cccactcgcc agggtaaatt
     3301 caccaaattt gacactcccc gtgtcgtaaa gaccttcaat cagttcagca aaacgcgaaa
     3361 ctttaggagc gtctaaaagc ttgatttgaa catcaattct gatgcgatta atctcatcgc
     3421 gcaaagcttc agcagctgct ttgggatctt ctgcgttcat aaccgagcgg gaaacgggga
     3481 caatcatgcc caaaccatcc gcgcgcaagc cattttcaag cgccaggcgc aagttgccac
     3541 cctgcgcgcc aacgcctggg gctaaaatcc aggcatccgg gcaaagttcg cgcacacgtt
     3601 tgagggcttc aggttgggtg gcaccaacca cgagacccac ttgctcagct gcaccccagt
     3661 gctgcgattg tttggcaaca tgctcaaaaa gagtcggtat gccatgctgc tggatttcct
     3721 tcgcgctggg attggatgtt tgacaaagca caaacaccgc tttatccgcg cggtcggtaa
     3781 aaggtttgat ggcgtcataa cccagatatg gtgagagcgt caccgcgtcc gcaccgtaga
     3841 tatcaaagca agcccgcgcg taagcttcag cagtggaacc gatatcaccg cgtttggcat
     3901 ctagcaatac aggcacatcg gcaggaataa gttcacgaca gatgacctga agtgcctcaa
     3961 ggccttcaag cccaaattgt tcaaaaaaag ccatgttagg tttgtaacaa caaaccagac
     4021 ccttggtctg ggagataata ttccctgccc aaagcaaaag cttttcctga atgttgtcac
     4081 cttcaggcag attcttctcg ttagggtcca gtcccacaca gagcaatgat tggtttttct
     4141 caatagtttg tgtaagtttt tcaataaagc tcatttcatc tcctgtctaa ttgcttgtgt
     4201 ttacgcgtat tctgttcaaa cactataaaa tttccggttc aaagt
//
LOCUS       DAOSFC010000112         4244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_18837_length_4244_cov_4.827166, whole genome shotgun sequence.
ACCESSION   DAOSFC010000112 DAOSFC010000000
VERSION     DAOSFC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18837_length_4244_cov_4.827166"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="PK791_03825"
     CDS             <1..1758
                     /locus_tag="PK791_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOH92475.1"
                     /translation="NYKAKVRLDVLERLAYVPDGKYIDVTAITPTPLGEGKTTTTVGL
                     SQALGAHLGKTVVTAIRQPSQGPTFGIKGGAAGGGYSQVIPMEDMNLHMTGDIHAITA
                     ANNLLAAAIDARMFHESSQSDKALFNRLCPADKKGNRQFARNMLGRLERLGITERDPN
                     KLTDEEKSKFARLDIDPDTVTWRRVIDTNDRMLRGITVGQGPAEKGFELKTNFTIAVA
                     SELMAILALATDLKDLRERIARIVIGQSRSGEPVTADDLGVAGAMTVLMMETIKPTLL
                     QTLEGTPVMVHAGPFANIAHGNSSIVADKIALKLADYVITESGFAADLGMEKFFNIKC
                     RYSGNIPNAVVLVATVRALKMHGGGPKVVAGTPLAPEYVEENLELVKKGSENLLAHID
                     IARKFGVPVVVAINRFPTDTDAEIELVRKIAKEHPGVADAVASDHFSQGGAGAVELAK
                     AVIKACEEPSNFKFLYPSEMSLKDKIETIATEIYGADGVDYSPEAEERLKRYTELGYG
                     NLPICMAKTHLSISHDPTLKNVPKGFRIPIQSVSASVGAGFVYPLLGTMSTMPGLPTR
                     PVYYDIDIDFETGRIVGLG"
     gene            1931..3133
                     /locus_tag="PK791_03830"
     CDS             1931..3133
                     /locus_tag="PK791_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOH92476.1"
                     /translation="MSKKTIQKIIALVLVFLVLSACGFTSLLSKIAQQVQETSPTTEL
                     RTDATPTAKPALPEPAVPGFNPELEDNQDLLEALYEHVSPGVVSIQAAGMRSGGSQGT
                     GFVVDTQGHIVTNYHVVADQDTIEVHFQSGLKSYAEIVGMDMDSDLAVIKVDVDPSEL
                     VPLAFGDSDLVKVGQTVAAIGNPYGLSGTMTVGVVSARGRVLDSMRQSAGGGYFSSGD
                     TIQTDALINPGNSGGPLLNLRGEVIGVNRAIKTSGISITGDAINTGIGFAISSNVARK
                     VVPSLIEKGSFDYPYLGMTSLSNISLGVQEALDLPRSTGVYISDVVQGGPAQKAGLRG
                     GTQPSQVQGYYKGGDLIIAVDGIEVKDFSEMFNYIVVNKDPGDVVVFTIIRNGKEMDI
                     NLTIEARR"
     gene            complement(3232..3771)
                     /locus_tag="PK791_03835"
     CDS             complement(3232..3771)
                     /locus_tag="PK791_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOH92477.1"
                     /translation="MLNIDNALLVIIDIQGKLAEVVHESEKHNAAAMRMIAGAQALEI
                     PILLTAQAPHKIGHTTEQVRELLPTQHEFARMGFSIYTSPEIRLTLKASGRKQMILCG
                     FESHICVYQSALDLMEAGYEVYILTDAVSSRDPQNKKYILKELRGEGAHLINVETVFY
                     ALMRDPNHPAFRAISKIIR"
     gene            3818..>4244
                     /locus_tag="PK791_03840"
     CDS             3818..>4244
                     /locus_tag="PK791_03840"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HOH92478.1"
                     /translation="MKELKARDAFKVIGESIKLWADDWANQALVGLVAILCSFTIVLL
                     PAATFGVFYQARDLARGNRSGLMGFWQGFKSYFKQNLLWGIVNLLVYAFLIFVITFYW
                     LSSDTLKSSVPLGIAGAFFTVLVFWSIWQFLTLSCFFLQD"
BASE COUNT         1076 a         1049 c         1046 g         1073 t
ORIGIN      
        1 aactataaag ccaaagtacg cctggacgtg cttgaacgac tagcgtatgt gcccgatggc
       61 aaatacattg acgtaaccgc catcacccct acccctcttg gcgaaggcaa aaccacaacc
      121 acagttggat taagccaggc ccttggcgcg catctgggca aaactgtcgt caccgctatt
      181 cgccagcctt cgcaaggtcc aacctttggc atcaaaggtg gcgcggctgg cggaggttat
      241 tctcaggtca ttccgatgga agacatgaac ctgcacatga ccggcgatat ccacgctatt
      301 accgccgcta ataatctgct ggcagccgct attgacgcgc gtatgttcca cgaaagcagc
      361 caaagtgata aggctttgtt taaccgcttg tgtcccgctg ataaaaaagg gaatcgccag
      421 tttgcccgaa acatgcttgg acgtttggaa agattaggca ttactgaacg cgaccccaac
      481 aaactgaccg acgaggaaaa aagcaaattc gcgcgtttgg acatcgaccc tgataccgtc
      541 acctggcgtc gcgttataga caccaacgac cgcatgttgc gtggcatcac ggttggccag
      601 ggccccgccg aaaaaggttt tgaactcaaa acaaacttca ccatcgcggt ggcttctgaa
      661 ctgatggcta ttctggcgct tgccaccgac ctgaaagact tacgcgaacg tatcgcgcgc
      721 atcgtgatcg gacaaagccg ttcaggcgaa cctgttaccg ctgatgactt gggtgtggct
      781 ggcgcgatga ctgtgttgat gatggaaacc attaaaccca ctctcttgca aacccttgaa
      841 ggcacgcccg tgatggtgca cgccggcccg ttcgccaaca tcgcccatgg caacagttcc
      901 attgtggctg acaaaatcgc attgaaactg gcagattatg tcatcaccga atcaggcttt
      961 gcggctgatt tgggcatgga aaaattcttc aatatcaagt gtcgctattc cggcaatatc
     1021 cctaacgcgg tggttctggt agcgaccgtg cgcgccctga agatgcacgg tggcggaccc
     1081 aaagttgtgg caggcacacc cctggcaccg gaatacgtcg aagaaaacct tgaactggtc
     1141 aaaaaaggtt ccgagaacct gttggcgcac attgacatcg cccgcaaatt tggcgtgccg
     1201 gtcgttgtag caattaaccg cttccccacc gacaccgacg ccgaaatcga acttgtgcgc
     1261 aaaatcgcca aagaacaccc gggagtcgca gacgcggtcg cttcggacca tttcagtcag
     1321 ggtggcgctg gcgcggtaga gttggccaaa gcggtcatca aagcctgcga agaaccgtcc
     1381 aacttcaaat tcctgtaccc gtcagaaatg agcctgaaag acaaaattga aactatcgcc
     1441 acagaaatct acggcgcgga cggcgttgac tacagccccg aagctgaaga acgcctgaag
     1501 cgttacaccg aactgggcta tggcaatttg ccgatttgca tggcgaaaac acacctttcc
     1561 atcagccatg accccacact caagaacgtg cccaaaggct ttagaatacc cattcagagt
     1621 gtgagcgcgt ctgttggcgc tggatttgtt tacccgctcc ttggcaccat gtccaccatg
     1681 cccggcttgc ccacccgccc ggtttactac gatattgata tcgactttga aacaggacgc
     1741 attgttggct taggctaaga ttatcgcttt tcaaacgccc ttgaaaccat tgcattttca
     1801 agggcgtttt tcttttcctg acattcttac ccatcatttt tatctgtaat acgaaaatta
     1861 gaatgagaaa tttaatgaaa actatacatt ccaggcgtag aatataatca gatattcaag
     1921 tgaggtagta atgtcaaaga aaacaataca aaagattatt gcgttagtgc ttgtgtttct
     1981 tgtacttagc gcatgcggat tcacatcact gttgagcaaa attgcccaac aagtccagga
     2041 aacatctcca accacagaat tgcgtactga cgcaacgccc acagctaaac cagccttacc
     2101 ggaaccggca gtcccaggtt ttaatcctga actggaagat aatcaggatc tgctggaagc
     2161 actttacgaa cacgtcagcc ctggtgtcgt ttctattcaa gccgctggaa tgcggtctgg
     2221 tggctcgcag ggtactggct ttgtcgttga cacccaggga catatcgtca ccaactatca
     2281 tgttgtagca gaccaagaca cgattgaagt gcattttcaa tctggtttaa agagttatgc
     2341 cgagattgtg ggcatggata tggattcaga cctggctgtt atcaaagtcg atgttgaccc
     2401 cagcgagctt gtaccgcttg cttttggcga cagtgacctg gtgaaagttg gccaaacggt
     2461 agctgctatc ggaaatcctt atggcttaag cggaacgatg accgttggcg tggtatccgc
     2521 ccgtggtcgt gtattagact caatgcgaca atctgcaggt ggcggttatt tctcatccgg
     2581 ggataccatt caaaccgacg cgctgattaa ccccggcaac tctggtggtc ccctgctcaa
     2641 cctgcggggc gaagtgattg gtgtaaaccg ggctataaaa acttccggta tttctattac
     2701 tggcgatgcc attaacaccg gcatcggatt tgcgatttca tcaaacgtag cgcgcaaggt
     2761 tgtgccatct ctgattgaaa aaggcagttt tgactacccc tatttaggca tgaccagcct
     2821 gagcaacatc agtttaggtg ttcaggaagc gctcgattta ccacgttcaa caggtgttta
     2881 tatcagtgac gtagtacaag gcgggccagc acaaaaagcc ggtctgcgtg gcggaaccca
     2941 acccagccag gtacagggtt attacaaagg tggcgacttg attattgctg tggacggcat
     3001 tgaagtcaaa gactttagcg aaatgttcaa ctatatcgtc gtaaacaaag atcccggtga
     3061 tgtggtcgtc tttaccatta ttcgaaatgg caaggaaatg gacatcaacc tgaccatcga
     3121 agcccgcaga tagcccctaa acctttcccg ctgtttgaag aaattatcaa aaaaccgcgc
     3181 tctcaacagg tgcggtttta cttttaactt attgagactt tgggaaaaga cttatctgat
     3241 aatctttgaa atcgcgcgga aagcagggtg atttgggtcg cgcatcaagg catagaagac
     3301 ggtttcaacg ttgataagat gcgccccttc gccacgcaat tcttttagga tatatttctt
     3361 gttctgggga tcacgcgaag acaccgcgtc agtcaaaata tagacctcgt agccggcttc
     3421 catcaggtca agcgcgcttt gataaacaca gatgtggctt tcaaagccac ataaaatcat
     3481 ctgtttgcgt ccggaagctt tgagcgtaag ccggatttca ggactggtat agatgctaaa
     3541 gcccatacgg gcaaattcat gctgcgtggg gagcagttcg cgcacctgtt cggtagtgtg
     3601 gccgattttg tggggagcct gggcggtgag caggatgggt atttccagag cttgcgcgcc
     3661 ggcaatcatt ctcatcgcgg ccgcgttatg tttttcgctt tcgtgaacga cttcggcaag
     3721 cttgccttgg atgtcaataa tcaccaaaag cgcgttatca atgttcaaca tagtcattcc
     3781 ttcatatatt tttgcttgtt tcaattataa tcataacatg aaagaactta aggccagaga
     3841 cgctttcaag gttatcggcg aatccatcaa actatgggcg gacgactggg caaaccaggc
     3901 tttggtcgga cttgttgcta ttttgtgcag tttcaccatc gttctcttgc cggcagctac
     3961 ctttggcgtc ttttaccagg cacgcgattt ggcaaggggt aatcgcagtg ggctgatggg
     4021 cttttggcaa ggattcaaaa gctattttaa gcaaaactta ctgtggggca tcgtcaatct
     4081 tctggtctat gcctttttga ttttcgtgat tacgttttac tggttgagtt ccgacacctt
     4141 aaaatccagt gtgcctttgg gtatcgcggg agcatttttt actgttttgg tgttttggag
     4201 catttggcaa tttttgacgc tttcctgttt ctttttacag gatg
//
LOCUS       DAOSFC010000113         4233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_18916_length_4233_cov_5.346099, whole genome shotgun sequence.
ACCESSION   DAOSFC010000113 DAOSFC010000000
VERSION     DAOSFC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18916_length_4233_cov_5.346099"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="PK791_03845"
     CDS             complement(<1..936)
                     /locus_tag="PK791_03845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOH92479.1"
                     /translation="MKKANIWVVLSLAIITVAVFVVFIFPNKAGSADEAMVNIFQPDE
                     YATWTVVDRMTSPKPDRPSFLQHYFLYDFYHYGFPYFSLSTIPVFVARWTHQIDNMSF
                     VMASIRQFISVLPSLIALLILVYIQDQFHSYKSILLYIILLSVPAVVNNNMWWHPDGL
                     CMLFSALTLFFLWKDEQKFGKHFYIAAIFAGILTATKLIGVFFLVAIIPILLGQLKAK
                     KWRFVELVGQWSLFAFIMAIAFVISNPFLFTFDGIKKYGFTLYNEFTQISAGYGLVYS
                     KGFAAAWAGIQPAYGKLPFFIAIAFSFVLGFLDKNK"
     gene            complement(1074..2300)
                     /locus_tag="PK791_03850"
     CDS             complement(1074..2300)
                     /locus_tag="PK791_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOH92480.1"
                     /translation="MTNPWEEKFSKRTAWMKSSFIRELLKVTNQPDVISFGGGFPAGE
                     LFPVERVKEACEKILTENPQKALQYSTTEGYDPLRQYLVDDYRSRGVNIGLQNIQITG
                     GSQQALDFIGRIFVNEGDKLLVESPTYLGALQAWNAYGPEYVTVETDDDGMQVDKIEA
                     IIKEHDPRFIYVLPNFHNPMGVSMSIERRKQLVEISHKYNLPIVEDDPYGKLIFEGEM
                     LPSIAKLDCQFRKGVDNFEGNVIHTSTFSKILAPGLRIGWAIGPEEVIKKMVQAKQGA
                     DLQCSTFDQYIAYELVAGDWLPDHIELIKRVYKERRDAMLQAFEDFMPEGTTWTTPAG
                     GLFLWLRLPEGCDSLELFPKAVEQRVAYVPGVPFYPIDGPRNTMRLNFSASDPEKIRE
                     GVRRISVMAKNALAGK"
     gene            complement(2328..>4233)
                     /gene="gcvT"
                     /locus_tag="PK791_03855"
     CDS             complement(2328..>4233)
                     /gene="gcvT"
                     /locus_tag="PK791_03855"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOH92481.1"
                     /translation="TWLTQRGFREAEMEEIAGLIVKVLDATKTYPMPARSGEKSRAKV
                     DFCTMNMVNDAVRKLADSKPSSDEIPTHHGYPHYFYASDKPAEGYGAFQLTGEGVRTF
                     LNYTLTNDIEALDPGQSQPTRMHTPCGDIDATLTCVSAKVFKLTIPAEKVGLAGTWLN
                     GISTGFVYYDEDLAIRIPGPISILPTEPEEYIEEGERVCDDKPWYIAMPKDISKPALP
                     EFVWKEPQNEELKRTVLHGTHIAMGAKMVPFAGFDMPVWYSSVMEEHRAVREAAGLFD
                     VTHMGVFQCEGPDAAVFLDSVSGNDTIFLEIGESVYSHFLDPHGKVIDDFIIYRRDED
                     RYLLVVNAANEEKDWAWLTAVKNGEVLVDLEKPWVKVPGRKCTLRNLKDPAAGEDQFV
                     TLAIQGPASRDILLSLACDHCNDHKIMRLGRGELCDSRLGNIPVIISRTGYTGEALGF
                     EVFVHPDNAVQLWKDLLEKGKDYGLVACGLAARDSLRTEAGLPLYGHEMGGDFNLTVG
                     DAGFISFVRFNSAWFIGRAPSMEYEKKRKSEVARFTFNEKGVRMARPGDPVCDLRGRV
                     VGYVTSCAIDQNGMLTGQAYVDKKVGKPGTSIHIFQNAPEWQQKPPAELVNGDRVAIP
                     STATIVKRFL"
BASE COUNT         1089 a          947 c         1111 g         1086 t
ORIGIN      
        1 cttgtttttg tccagaaatc ctaagacgaa ggaaaaagcg atagcaatga aaaaaggcaa
       61 tttgccataa gcaggttgaa tgcctgccca ggcagctgca aaacccttgc tgtagaccaa
      121 gccatagccc gcagagattt gtgtgaactc attatataag gtaaagccgt acttctttat
      181 accgtcaaaa gtaaacaaaa acgggttcga aatgacaaag gcgattgcca tgatgaaggc
      241 gaaaagtgac cattgcccga ccagctcaac aaaacgccac tttttagctt tgagttgacc
      301 taataggatg ggaatgatgg cgacaaggaa aaagacgccg atgagtttgg tggcggtcag
      361 gataccggcg aatatcgcgg cgatataaaa atgctttcca aatttttgtt catctttcca
      421 caggaaaaag agcgtgagcg cgctaaacag catgcacaaa ccgtctggat gccaccacat
      481 attgttgttg accactgcag ggactgataa gagaataatg tagagcagaa ttgacttgta
      541 gctgtggaac tggtcttgaa tatataccaa aatgagaagg gcgattaagc tgggcaaaac
      601 gctgatgaat tgtcttatgc ttgccatgac gaaagacata ttgtcaatct gatgggtcca
      661 tcgcgcgaca aagacaggga tcgttgataa tgaaaagtat ggaaagccgt aatggtagaa
      721 gtcgtagaga aaatagtgtt gtaaaaacga cggacggtct ggtttggggg atgtcattct
      781 gtcaacaacg gtccaggtag cgtattcatc tggctggaaa atattcacca tggcttcatc
      841 tgctgaacca gctttgtttg gaaaaatgaa cacgacaaaa acggcaacgg tgatgattgc
      901 gagacttaaa acaacccaaa tattagcttt tttcatagct tgaattatac ctgttaatca
      961 atggcaagtt tataagcata gcaactttct tttttagcaa attaattgtt tctaaacgaa
     1021 caaaaagccg cgttttaacg cggctttttg ctatctctaa tctggaaaaa gctttacttt
     1081 ccagccaaag cgtttttagc catgacggaa atacggcgga caccctcgcg aatcttttcg
     1141 gggtcgctgg cgctgaagtt aaggcgcatg gtgttgcggg ggccatcaat cgggtagaat
     1201 ggcacaccag gcacataagc aacgcgctgt tcaacggctt tggggaagag ttcgagcgag
     1261 tcgcagcctt cgggcaagcg cagccaaagg aaaaggccac cagcaggggt tgtccaggta
     1321 gtgccttcgg gcatgaagtc ttcaaaagcc tgaagcatcg cgtcgcggcg ttctttgtaa
     1381 acgcgtttga taagttcaat gtggtcgggc agccagtcac ctgccacaag ttcgtaggcg
     1441 atatactggt cgaaggtcga gcattgcagg tcagcacctt gtttggcctg aaccatcttc
     1501 ttgatgactt cttcaggtcc tattgcccaa ccgatgcgca agccaggggc taaaatcttg
     1561 gagaaggtac tggtgtgaat aacatttcct tcgaaattat ctacaccttt gcggaactgg
     1621 caatcgagct tcgcgattga tggaagcatt tcgccttcga aaatcagctt gccgtagggg
     1681 tcgtcttcaa cgatgggcag attgtattta tgtgatattt caacgagctg tttgcgtcgt
     1741 tcaatggaca tggacacacc catcgggttg tggaagttag ggagcacata aataaatctg
     1801 gggtcatgtt ccttgatgat ggcttcaatt ttatcaactt gcatgccgtc atcatcggtc
     1861 tcaacggtga cgtattccgg gccataagcg ttccaggcct gaagcgcgcc gagataagtg
     1921 gggctttcga caagcagttt atcgccttcg ttgacgaaaa tgcgaccgat aaagtcaagc
     1981 gcttgttgtg agccaccggt gatttgaatg ttttgcaaac cgatattcac accgcgtgag
     2041 cgatagtcat caacaagata ctggcgcaag gggtcatagc cttcggtggt gctgtactgg
     2101 agtgcttttt gcggattctc agtgagaatt ttctcgcaag cttcttttac ccgttctacc
     2161 gggaaaagtt caccagctgg aaaaccaccg ccaaatgaaa tgacatcagg ttgattggtg
     2221 actttgagca gttcgcggat gaatgagctt ttcatccaag cggtacgttt agaaaatttt
     2281 tcttcccatg gattagtcat aatatgtgtc tcctctcaaa ctattgttta taaaaatctt
     2341 tttacaatcg ttgccgtact gggaattgcg acacgatcgc cgtttactag ttcagcgggt
     2401 ggtttttgtt gccactcggg cgcgttttgg aagatgtgga tgctggtgcc aggtttgcca
     2461 accttcttgt ccacataggc ctgtccggtg agcataccat tctggtcgat ggcgcagctg
     2521 gtaacgtagc caacaacacg tccgcgaagg tcacaaacgg ggtcacctgg gcgagccatg
     2581 cgcacgcctt tttcgttgaa ggtaaagcgt gccacttctg atttgcgttt cttttcatat
     2641 tccatgctgg gcgcgcgtcc gatgaaccag gcagagttga agcggacaaa ggaaatgaaa
     2701 cctgcgtcgc caacggtaag gttgaagtca ccacccattt cgtgtccgta cagaggcaag
     2761 ccagcttcgg tgcgcaggga gtcgcgcgcg gcaagaccgc aggcaaccag gccatagtcc
     2821 ttgccttttt cgagcaagtc tttccaaagc tgaacggcat tatcggggtg aacaaagact
     2881 tcaaagccca aagcttcgcc ggtataaccg gtgcgcgaga tgattaccgg gatattgccc
     2941 aaacgactgt cgcaaagttc acctctgccc aggcgcataa tcttatggtc attgcagtgg
     3001 tcgcaagcca aactgagcag aatatcgcga ctggctggtc cttggatggc aagggtaaca
     3061 aattgatctt cgccagcagc ggggtctttg agattgcgca aggtgcattt tctgccgggg
     3121 actttaaccc agggtttttc aaggtcaacc aaaacttcac cattctttac cgcggtgagc
     3181 caggcccagt ctttttcttc gttggcagcg ttcacaacca ggagatagcg gtcttcatca
     3241 cggcggtaaa tgataaagtc atcgataact ttgccgtggg ggtcaaggaa gtgcgaatag
     3301 actgattcgc cgatttccag gaagatggtg tcatttccgg aaacagagtc caggaagact
     3361 gctgcgtcgg ggccttcaca ctggaatacg cccatgtggg tgacatcaaa aagaccggca
     3421 gcttcgcgta ccgcgcgatg ttcttccatt acagagctat accagacggg catatcaaag
     3481 ccggcaaagg gtaccatctt ggcgcccatg gcaatatgcg ttccatgaag tacggtgcgt
     3541 ttgagctctt cgttttgggg ttctttccaa acaaattccg gtaaggcagg tttgctgata
     3601 tctttgggca tggcaatgta ccatggtttg tcatcgcaaa cgcgttctcc ttcttcaatg
     3661 tattcttccg gttcagtggg taagatagag atggggccag gaattctgat agccagatct
     3721 tcatcatagt agacaaaacc cgttgaaatg ccatttagcc aagtgccagc cagtccaact
     3781 ttctctgctg ggatcgtgag cttaaagact ttggccgaaa cacaggtcag tgtcgcgtca
     3841 atatcgccac aaggggtgtg catgcgggta ggctgggatt gtccggggtc aagggcttca
     3901 atgtcgttgg taagagtgta gttaaggaaa gttcgtacac cttcgccggt aagttggaac
     3961 gcgccatagc cttcggctgg cttatcgctg gcatagaagt aatgcgggta gccatggtgg
     4021 gtaggaattt cgtcgcttga aggtttggaa tcggccagtt tgcgaacagc gtcgttgacc
     4081 atattcatgg tgcagaagtc caccttggcg cgtgattttt cgccactgcg ggcgggcatg
     4141 gggtaggtct tggtagcatc caaaacttta acaattaagc ccgcgatttc ttccatttct
     4201 gcttctctga aaccacgttg ggtgagccat gtt
//
LOCUS       DAOSFC010000114         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_19171_length_4190_cov_4.349456, whole genome shotgun sequence.
ACCESSION   DAOSFC010000114 DAOSFC010000000
VERSION     DAOSFC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19171_length_4190_cov_4.349456"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1230
                     /locus_tag="PK791_03860"
     CDS             199..1230
                     /locus_tag="PK791_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOH92482.1"
                     /translation="MKDIRLLMIGFGNVGRAFARLLMQKQSTLENDYGIRAKVTGIKT
                     ARHGACINANGIDLNKALHLVESKQDLGVLSTQSFKGDTFDFIKTCPADVLIETTPVN
                     PQAGTPAIDHLRTGFECGMHGITANKGPVVYAYQKLNELAQSKGLKFRFESAVMDGAP
                     IFSLHRHTLAGAEIEGFVGILNSCTNLLIEAMEDGTSLEDAVRHAQEIGIAESDPSND
                     IDGWDAAIKVAALSTVLMNHPITPLEVDRTGIRGISSQMIADAKLQGKRWKLVCRGGW
                     ENDKFIAQVKPEMVGTDSPLFSVAGTSSFVSFALDVLPGLGILESDPSPKTTAFGLLA
                     DLIDIYKGA"
     gene            1230..1715
                     /gene="folK"
                     /locus_tag="PK791_03865"
     CDS             1230..1715
                     /gene="folK"
                     /locus_tag="PK791_03865"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOH92483.1"
                     /translation="MEDVFLGIGSNLGDREENLAQARGSIEAFARIEAVSPVYETDAW
                     GFEDQPAFLNQVIHIKTSLSPQALLNRIKRIEKDLGRKKSFRWGPRLIDIDILFFADY
                     IIESASLTIPHKELHKRAFVLVPLADIAPDFVHPVFNQTVSALRDTLADLEGIRLWKS
                     S"
     gene            1700..3553
                     /locus_tag="PK791_03870"
     CDS             1700..3553
                     /locus_tag="PK791_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92484.1"
                     /translation="MEILLVLRTILAYIAVLLPGLAFWAWFHNDEDDPAQSLAQILGI
                     SFSVIALGALVFHYIGLRVNGWVLGIFLAICLVLAVIGIIKTKPKFVWWHWLIMAGIL
                     GGFIYWRLWQARSLVLPNWVDSQHHVLIIRKFIEAGGLPASLEPYLPGPFYYHFSFHS
                     VVAYFASISGQNPALATLIFGQVLNALIGLGVYTLTKSLAKDWRPALIAAIFVTFVTK
                     MPGYYLTWGRYTLLTGTFLLALAMAFAYKIYSGERNTGNTVTLGLLTAGALLSHYLIA
                     FMLALFLVILGFAWIIDSIKAKQWDFKILLSLLIPALSGLVVSLPWYLHVLALSKGRY
                     SVGISATRGHFLPKPEDWSYITYVLGPVAAYALIALALIGLVVAFCKSEYRKIAFWSL
                     IITLFTIPTGIEFMGFRGDYFALITFIPITILSALLVFWVAEAIQKRTSHEKFANIFL
                     VVVTLTMFFWGIGQNKKAINSSTVIAIQDDVEAMNWINENLPEDARFYVNMIYWGYGV
                     SRGVDGGGWLLPYTGRFSVAPTTFYPYGMKAEDIELWMSWGMRASQITECDESFYSLL
                     ADADLNYIYVRDGVRPNNQENVAGIQSQTLLACPGITQLYQNDSVSIWKLD"
     gene            complement(3575..>4190)
                     /locus_tag="PK791_03875"
     CDS             complement(3575..>4190)
                     /locus_tag="PK791_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92485.1"
                     /translation="VADAHFLEALIAGSQKAGFEITAPLIYYYAEPDEVWSAGGDICK
                     LIGLPLDAHHRKDPPAEAVKRTFLTGCCLLFSRHALESLGLFDEAYFMYYEDLDLMAR
                     ALKVKTCVGVVPSAKLWHKEAQSSGGYLNPNERYWSARSLMRYSKRATRWWNAVPLWA
                     HRLISIALWTFRLLLKRKPEALKAYWQGIRDGLRTKQADRLVSK"
BASE COUNT         1041 a         1021 c          955 g         1173 t
ORIGIN      
        1 gtgaagaaaa catcaaaatt gttgattctc tggacgcctg ctaatttatc gtttcctttt
       61 aatttctcat ttacaaaaac accccttaac tcagcactta caaaggcata ttagcaccgt
      121 aaaaccattt aaccctacat ccttacccaa gtttagctat gtttggctat aattacgaaa
      181 actttgtgag gtgagatgat gaaagatatt cgtttgctaa tgataggttt tgggaatgta
      241 gggcgagcct ttgcgcgcct gctcatgcaa aaacaaagta cattagaaaa tgactatggc
      301 atacgcgcca aagtaactgg catcaagacc gcacgccatg gcgcgtgcat caacgccaac
      361 ggtatcgatc ttaataaagc tttgcatctg gttgaaagca aacaagatct cggtgtactc
      421 agcactcaaa gttttaaagg cgataccttc gacttcatca aaacttgtcc tgcggatgtt
      481 ctcattgaaa caacgcctgt caatccgcaa gcgggcacac cagccataga tcacctgcga
      541 acaggttttg agtgtggtat gcatggtatc accgccaata agggacccgt tgtttacgcc
      601 taccaaaagc taaacgagct tgctcagtca aaaggactca aattccgctt tgaatccgcg
      661 gtcatggatg gcgcgcctat cttttcctta caccgccaca ccttggctgg ggctgagata
      721 gaaggtttcg tgggtattct caactcctgc accaatctgc ttatcgaagc gatggaagat
      781 ggcacatccc tcgaagacgc tgtccgtcac gcccaggaaa tcggcatcgc cgaaagcgac
      841 ccaagtaacg acatagacgg ttgggacgct gccatcaaag tcgccgcgct ttctaccgtg
      901 ctgatgaatc accccatcac acctttggaa gttgaccgta ccggcatccg tggcattagc
      961 agccaaatga ttgctgatgc taaattacag gggaaacgct ggaaattagt ctgccggggt
     1021 ggctgggaaa acgacaagtt cattgcccag gtaaaacccg aaatggttgg cactgactcg
     1081 ccacttttta gcgtggcagg cacctcatca tttgtgagtt tcgccctgga tgtgctgccc
     1141 ggattaggca tcttggaatc tgaccccagc cctaaaacca ccgcttttgg tttattagct
     1201 gatttgattg atatttacaa gggcgcgtaa tggaagacgt ttttctgggc atcggttcga
     1261 accttggtga ccgtgaagaa aacctggcgc aagcgcgcgg ttcaattgaa gcattcgcgc
     1321 gtatagaagc tgtttcgcct gtatatgaaa ccgatgcctg gggttttgaa gaccagccag
     1381 cttttttaaa tcaagttata cacataaaaa cctctctctc accacaagct ctgcttaatc
     1441 ggataaaacg cattgaaaaa gatctcgggc gaaagaaaag ttttcgctgg ggtccgcgtc
     1501 taattgatat agacatttta ttttttgctg attacattat cgaaagcgct tcgcttacca
     1561 ttccccacaa agaacttcac aaacgcgcct tcgttttggt gcctttggct gacatcgcgc
     1621 cagattttgt gcacccggtg ttcaatcaaa cagtttcggc acttcgcgat accttagccg
     1681 acctggaagg gataagatta tggaaatcct cttagtttta cgaacaattc tcgcatatat
     1741 cgcggttctt ttgcctggac tggcgttttg ggcatggttc cacaatgatg aagacgaccc
     1801 cgcgcaaagt ctggcacaaa tcttgggcat cagctttagc gtgattgcgc ttggtgcttt
     1861 ggtgtttcat tatattggct tgcgtgtcaa tggttgggtc ctggggattt ttctggcaat
     1921 ctgcttggtc ttagctgtta ttggcattat caaaaccaaa ccaaaattcg tttggtggca
     1981 ttggctcatc atggcaggca ttttaggtgg attcatctat tggcgccttt ggcaagcgcg
     2041 gtcgctcgtc ctgccaaact gggtcgactc acaacaccat gtgctcatca ttcgcaagtt
     2101 cattgaagca ggcggactgc cagccagcct tgaaccctat ctaccgggtc ctttctatta
     2161 ccacttctcg tttcacagtg tcgtcgctta ttttgccagc atcagtggac aaaaccccgc
     2221 cctggcaacg ctcatttttg ggcaagtgct caacgcgctg attgggctgg gtgtttacac
     2281 ccttaccaaa agtctcgcca aagactggcg tccggcactt attgcggcta tattcgtcac
     2341 ctttgttacc aaaatgcctg gttattacct gacctggggt cgctacacct tgctcacggg
     2401 tactttcctt ttggctttgg caatggcttt tgcctataag atttattcgg gtgaaagaaa
     2461 cacggggaac acggtaacgc ttggattgct cacagccggc gcgctcttaa gccactatct
     2521 cattgccttt atgttggcac tgtttctggt gattcttggc ttcgcctgga ttattgatag
     2581 cataaaagca aaacagtggg attttaaaat actcttatcc ttgctcattc ctgctttgtc
     2641 gggtcttgtg gtcagtctgc catggtattt gcatgttctg gcactttcca aaggccgcta
     2701 ttcggttggc atcagcgcta cccgaggcca ctttttaccc aaaccagaag attggtcata
     2761 cattacgtat gttttaggcc cagtcgcggc gtatgcgctc atcgcgctgg cactcatcgg
     2821 cttagttgtc gcattttgca agtcggaata tcgcaaaata gcgttttggt cgctgattat
     2881 cacgctattc actatcccaa caggcattga atttatgggc ttccgcggtg actattttgc
     2941 tctgattact tttataccga ttaccatatt gagtgcttta cttgtatttt gggtcgctga
     3001 agccatacaa aaacgaactt cgcatgaaaa gtttgccaac atttttcttg tagtcgtcac
     3061 actcaccatg ttcttttggg gcataggtca gaataaaaag gccatcaata gttccactgt
     3121 catcgccata caagatgacg tggaagctat gaactggata aatgagaatc tgcccgaaga
     3181 cgcccgtttt tatgtaaaca tgatttactg gggctatggt gtctcgcgcg gtgttgatgg
     3241 cggtggttgg ttgttacctt atacggggcg atttagtgtc gcgccaacaa ctttctaccc
     3301 ttacggaatg aaagctgaag atatcgaatt gtggatgtca tgggggatgc gcgcttcaca
     3361 aataacagaa tgtgatgaat ctttctatag tttgctggct gacgctgacc taaactatat
     3421 ctacgtgcgc gatggtgttc gtcctaacaa tcaggaaaat gttgccggaa ttcaaagcca
     3481 aaccctgctt gcctgcccag gtattaccca gctttatcaa aacgacagcg ttagcatttg
     3541 gaaactggat taacgacgag ataaataagc ctgattattt gcttacaagc cggtcggcct
     3601 gcttagtgcg caaaccatcc cgaatgcctt gccaataggc ttttaaagct tcgggttttc
     3661 tttttaatag cagacggaaa gtccacaagg ctatgcttat tagcctatgt gcccaaagtg
     3721 gaacagcgtt ccaccatctt gtggcgcgtt tgctatagcg cattaatgag cgtgccgacc
     3781 aatagcgttc attcgggttc aaatagcccc cactgctttg ggcttcctta tgccacaatt
     3841 tcgcgctggg cacgacaccc acacaggttt ttaccttcag cgcgcgcgcc ataagatcca
     3901 ggtcttcata atacatgaaa taagcctcgt caaaaagccc caggctttcc aaagcgtgcc
     3961 ggctgaacaa aaggcaacaa cccgtcaaaa acgtcctttt gacagcttct gcaggtggat
     4021 ctttgcgatg atgcgcatca agcggcaagc caattagttt gcaaatatcg ccccctgctg
     4081 accacacttc atcaggttct gcatagtaat aaatcaaagg tgctgtgatt tcaaaaccgg
     4141 ctttttgact tcctgctatg agtgcttcta aaaaatgggc atccgccacc
//
LOCUS       DAOSFC010000115         4097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_19798_length_4097_cov_4.927759, whole genome shotgun sequence.
ACCESSION   DAOSFC010000115 DAOSFC010000000
VERSION     DAOSFC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19798_length_4097_cov_4.927759"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PK791_03880"
     CDS             complement(<1..549)
                     /locus_tag="PK791_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92486.1"
                     /translation="MSIYRVIILLVIISFLFNSCSLVSKLPTDEAEIAILCKSKKIIR
                     IHEIQGVGHRSPLENSTIQCVTGIVTAVSGTGFYLQDLIEDGDDRSSEAIFVAHSSGS
                     MVKKGDRVLVYQAMVKEHKPTLGQLNSLSITQLVNPKFEVLSSGHPLPKAVILGKGGR
                     VIPNLIIENDVQGKTLDDYRFLM"
     gene            740..1759
                     /locus_tag="PK791_03885"
     CDS             740..1759
                     /locus_tag="PK791_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH92487.1"
                     /translation="MNEDFILITGANGEVGHGLIKHLAKVGHKKIIAFSRRPLSEELQ
                     PFVSHPLAMDVQDLAGLTELFENHNITTVYHLASILSTKAEHDPVLAHNINVNGTMNL
                     LKLCAMKMEKTGKPVKFIYPSSIAVHAIPNLEVKAQAGKVKENDYVQSTTMYGINKLY
                     CENLGRYFNSHYKQLSYAPDERPYVDFRALRFPGLISADTMPTGGTSDYGPEMIHAAA
                     SNRHYNCFVRPDSTLPFMVMPDAIKSLVMLHAADRKRLTQTVYNVTSFSPTAQEIYEV
                     TKAAFPNADIDFVPDVNRQGIVDSWPADIDDSAARKDWDWQPDYDFERAINEYLIPAI
                     RQRYA"
     gene            1990..2838
                     /locus_tag="PK791_03890"
     CDS             1990..2838
                     /locus_tag="PK791_03890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH92488.1"
                     /translation="MKKARKYFENKNVLITGGSSGIGFSIAQELMQMGASVCLLARNQ
                     EKLEEAKHKLQVLAVKPQQTVEILSADVADTERLCEIIPAYLKDYPVPDVLINAAGVA
                     RPGYVEELDLDIFKWTMDIDYHGTVTMTKLLLPHFLERGSGHIVNFSSLAGVLGVFGY
                     TAYSGAKFAVRGFTDVLRAEMKPRGINVSIVYPPDTDTPQLAWESQFKPYETHIIANS
                     DKPISAELVAHSVLKAVSRKKYAIVPGIDAKLTYLLGTTLGNLVYPIMDILVSSAIKK
                     KKKANK"
     gene            2851..>4097
                     /locus_tag="PK791_03895"
     CDS             2851..>4097
                     /locus_tag="PK791_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH92489.1"
                     /translation="MDLNQVLEKWKSSQSFTDNVSAWIVTPAVEAKAFPFPENLAPKL
                     TGALDKNGITHLYSHQLETFKSVEEGKNVVIATGTASGKTLAYNLPVINHIIQNPNAC
                     ALYIYPTKALAQDQLNNLNDLRNTANIQNIFIPAIYDGDTPQNQRITIRKSANILKTN
                     PDMLHQGILPHHTKWHRFLSNLRFVIIDEMHSYRGVFGSHFANVIRRLKRICAFYGAY
                     PQFILTSATIGNPQELAENLTETPVQVIDQDGSQQGEKQFIIFNPPLVDQQLGIRKSA
                     LNTAVNFSKELIANSIQTLIFARTRRTVEMTLSYLRDSLPIHLRDKVRGYRSGYLKDE
                     RREIERDFRESKLLAVASTSALELGIDIGDLESVILLGYSGSIATTKQRAGRAGRQNQ
                     SAVAILIPTPDALDQYLANHPGYL"
BASE COUNT         1174 a         1041 c          811 g         1071 t
ORIGIN      
        1 cattagaaat ctataatcat caagcgtctt gccttgaaca tcgttttcaa taattaggtt
       61 tgggataact cttccgccct tacccagaat aaccgctttg ggtaagggat gtccagagct
      121 aagtacttca aatttagggt taactaattg cgttatgctt agcgaattca actgccctaa
      181 ggttggcttg tgttccttaa ccatggcctg atacacgagc accctgtcgc ctttctttac
      241 catacttcca gaagaatggg caacaaatat tgcttctgaa ctgcggtcat cgccatcttc
      301 aattaaatct tgcagataaa atcctgtccc gcttaccgcg gttactattc cggtcacaca
      361 ctgaatcgtt gaattttcca ggggagatcg atggccaacg ccttgaattt catgaattcg
      421 gatgattttc ttgcttttac ataaaatggc aatttcagct tcgtctgttg gaagttttga
      481 tactaaactg catgagttga ataggaaaga aattattaca agcaaaataa taacgcggta
      541 gatactcatt gatttcttca tgagtttaat tttacttgaa ttcgacgata aacgtcatct
      601 tcgtggtata ttattctcag acatgacaaa taataagcac aaacgcgata gcttacattt
      661 aaacgaaact ttacaccatg ttttctacgc gttgtttttt attgtcaaaa attaaacata
      721 tctcgcaaag gagtattcga tgaacgaaga ttttattttg attactggtg ctaatggaga
      781 agttggacat gggcttatca agcatcttgc gaaagttggt cataagaaga taatcgcctt
      841 tagccgccgt cctctttcag aagaactgca accgtttgtt tcccaccccc tggcaatgga
      901 cgttcaggac ctggctggac ttacagaact cttcgaaaat cacaatatca ccactgtcta
      961 ccatcttgct tcaatccttt ccaccaaagc cgaacatgac cccgttctcg cgcataacat
     1021 caatgtaaat gggacgatga acttactaaa gctatgtgca atgaagatgg aaaagacagg
     1081 taaaccagtc aaattcatat atcctagttc aattgcggta catgcgattc ccaaccttga
     1141 agttaaagct caagctggca aagtcaaaga aaatgactat gttcaatcca caaccatgta
     1201 cggtatcaac aaactctact gcgagaatct tggccgttac ttcaacagcc actacaaaca
     1261 acttagctat gcgcctgacg aacgacctta cgtagatttt cgcgcgctac gcttcccagg
     1321 cctcatcagc gctgacacaa tgcccaccgg tggcaccagc gattacggtc ctgaaatgat
     1381 tcatgccgca gcgagtaaca gacattacaa ctgctttgtc cgtccagact ccaccttgcc
     1441 gttcatggtg atgccggatg ccattaagtc actcgtcatg ctccacgccg ctgaccgcaa
     1501 acgcctcacc cagactgttt acaacgtcac cagctttagt ccaactgcac aggaaattta
     1561 cgaagtcacc aaagccgcct tcccgaatgc ggatatcgac ttcgtgcctg atgtcaatcg
     1621 ccagggcatc gtagatagct ggcctgccga tattgacgac agtgccgccc gaaaagactg
     1681 ggactggcaa cctgattatg actttgaacg cgccatcaac gaatatctca tcccagctat
     1741 tcgtcaacga tacgcttaag caaggtgtag catgcctctt tacgaatacg agtgccaaag
     1801 ttgcggaaaa cattttgaac tattacgttc tatttgccag gcagatcagc cagcgaaatg
     1861 tcccgcttgt gcaaataatg acagcaaacg tttaatctca ctggtaaatg cctatagtgc
     1921 cggacgtaat cttggtcaag gcagtgccag ttgttcttca tgttcaagcc acaattgcgg
     1981 gagttgtaga tgaaaaaggc acgcaaatat tttgaaaata aaaatgtact gattaccgga
     2041 ggctccagtg gcatcggctt tagcatcgcg caagagctta tgcaaatggg agcctccgtt
     2101 tgccttttgg cacgcaacca ggaaaaactt gaagaagcca aacacaagct tcaagttctt
     2161 gctgtcaagc cacaacaaac agtcgaaata ctctccgccg atgtcgcgga taccgaacgc
     2221 ctttgtgaga tcattccagc ttacctgaaa gattaccccg tcccagacgt attgatcaat
     2281 gctgccggcg tcgcccgtcc gggttatgtc gaagaactcg atttagacat tttcaagtgg
     2341 accatggata tcgactacca cggcactgtg acgatgacca aactgctttt accccacttt
     2401 ttggaacgcg gcagtgggca tatcgtcaac ttttcatctc tggcaggtgt tttgggcgtt
     2461 tttggctaca ccgcttattc tggcgccaag ttcgctgtgc gtggtttcac agacgtttta
     2521 cgcgctgaaa tgaaacccag gggaatcaat gtctctatcg tttacccgcc cgacaccgac
     2581 acaccccagc tggcctggga aagccaattt aagccatacg agacgcatat aatcgccaac
     2641 tccgataagc ccatttccgc tgaactggtc gcccactccg ttctcaaagc tgtttcccgc
     2701 aaaaagtatg cgattgtacc cggcattgat gcgaaactca cttatctttt gggcacgact
     2761 ctgggcaatt tggtttatcc tattatggat atactcgttt ccagcgcgat taaaaagaaa
     2821 aagaaggcaa ataaatagcc tgcatctttg atggatctaa accaagtttt agaaaaatgg
     2881 aaaagcagtc aatcgtttac cgataatgta agcgcatgga ttgttacgcc agcggttgaa
     2941 gccaaagcgt tcccattccc tgaaaatctg gccccaaagc ttacaggtgc attagataaa
     3001 aacggtatca cccatcttta ttcccatcag ctcgaaacat ttaagtcagt agaagaaggt
     3061 aaaaatgtcg taattgcgac tggcaccgcc agcggtaaga cactcgctta caacctgcct
     3121 gtcatcaacc acattataca aaatcctaac gcctgcgcgc tttatattta tcctactaaa
     3181 gcgctggcac aagaccagct aaacaatcta aacgatttaa gaaacaccgc caatattcaa
     3241 aacatattca taccagccat ttacgatggt gacacacctc aaaaccaacg cattactata
     3301 cgcaaaagtg caaatatttt aaagactaac cccgatatgc tgcaccaagg catcttgccc
     3361 catcacacaa aatggcatcg ctttttatcc aatctgcgct tcgttattat tgatgaaatg
     3421 catagttacc gtggtgtctt tggttcgcat ttcgctaatg tcatcagacg gctcaaacgc
     3481 atttgcgcct tttatggcgc ttacccccaa ttcatcctca cctctgcgac catcggcaat
     3541 ccccaggaac ttgctgaaaa cctcactgaa actccggtcc aggtcattga tcaggatggc
     3601 tcacaacagg gcgaaaaaca attcatcatc ttcaacccac cacttgtcga ccaacaactc
     3661 ggaatccgca aaagtgccct aaacacggct gtaaacttca gcaaggaact gattgctaac
     3721 agcatccaaa cactcatttt tgcccgcacc cgacgcacag tagaaatgac cttaagctat
     3781 ttgcgcgatt cattacccat tcatctgcgc gataaagtac gcggataccg cagtggctac
     3841 cttaaagatg aaaggcgcga aatagaaagg gattttcgtg agtctaagct tttagcagtt
     3901 gcctcaacat cagcattaga attaggtata gatatcggtg acttagaatc agttatttta
     3961 cttggctatt cgggttcaat cgccacgacc aaacaacgcg ccggtcgggc tggtcgccaa
     4021 aatcaaagcg ccgtcgccat tctcattcct actcccgacg cgcttgacca atatcttgcc
     4081 aatcaccccg gttatct
//
LOCUS       DAOSFC010000116         4049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_20128_length_4049_cov_4.240861, whole genome shotgun sequence.
ACCESSION   DAOSFC010000116 DAOSFC010000000
VERSION     DAOSFC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20128_length_4049_cov_4.240861"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="PK791_03900"
     CDS             complement(<1..855)
                     /locus_tag="PK791_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92490.1"
                     /translation="MNIDIRSTIMTISILAVVAGLATASRARRTIVEGRKLSFFTKRR
                     HQIELGIRLIIGALVLFAFAYLNFRYGEGVMYTYFPPSLTPSLTPTITLTPTITLTPT
                     ITLTPTITNTPSVTNTPSVPDDIASQFESIITPGDNYALSGILFAREIDDGLQPIDPA
                     TEFENPLETIYACYSYNMMDEGVQWTELWYRDGDLIQSHTGRWQGGSGGYGVAKLELE
                     PSKWKPGEYRVLFFVGHGWVRSATFTVLGTPPTSTPTITPSQTATPTNTALPTSTPTR
                     TSTPWPTNT"
     gene            complement(921..2027)
                     /locus_tag="PK791_03905"
     CDS             complement(921..2027)
                     /locus_tag="PK791_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOH92491.1"
                     /translation="MQAFKTTPSEWNQLLLQFPEAHILQTWEWGASKIRNGWTPETFV
                     WYDEAQNIEACALILRRELKLGFLRLRVLYCPKGPNLDWANKALRERVLDDLQNYARD
                     QKAIFLKIDPDVLLGTGVPGAEDETVSALGVEFENELKGRNWLFSQDQIQFRNTVFID
                     LTRDEEEILAAMKQKTRYNIRLATRRGVSVRQASLADLPMLYQTYAYTALRDDFVIRG
                     EDYYLELWESFVEADMCHALIAEVEGEAVAGLFIFHFNGVARYMYGMSTDKYRELMPN
                     YLLQWEAIKLSKQLGCESYDMWGAPDIFDESDSLWGVYRFKEGFNGTTARHIGAWDYP
                     ARPLLYKLYTNVLPRLLDIMRRRGKQATRESLNE"
     gene            complement(2014..2994)
                     /locus_tag="PK791_03910"
     CDS             complement(2014..2994)
                     /locus_tag="PK791_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOH92492.1"
                     /translation="MTFSAFPASQHILQTDSWGEFKARYGWEPVRLHSGQESALVLFK
                     RLPLGLSVAYVPKAGIGEEWTRLLPKIETECRQRNAVFLQIEPDLDEPYDTAGLDSIF
                     PNYIKDEATIQPRGTILIDISSEETAILAAMKQKTRYNIRLAERKGIKVETSHDLDDF
                     YEQMLITSGRDGFALHSLPYYQNAFEIFEPKGQCVLLRASYEGESLAYLMLFLYGQRA
                     WYFYGASNDKHRNLMPTYLLQWEAMRYAKAHGATLYDMWGIPDADEDELEEQFMLRSD
                     GLWGVYRFKRGFGGEVKRSAPAYVKVFKPLLYKAYRWLRNKRTGGENASL"
     gene            complement(2996..>4049)
                     /locus_tag="PK791_03915"
     CDS             complement(2996..>4049)
                     /locus_tag="PK791_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HOH92493.1"
                     /translation="FIIVTALLTLIWRLIFIRVFKAVNQQKRVLIVGAGNAGTELIRE
                     INKLDPKPFNLIGLIDDDPLKLNTRIEGVPVLGDHKTMESITEKLGVSDFILAITHEM
                     NPGMLHTILDAQEAGLELTTMQDAYESITGRVPIDLLQPDWVIRSFLDRKPNSGFYRI
                     FKRLLDLFLAGLGLIGLAVVFPVIALGLLIEGNIPIIFKQERLGRGGKPYTIYKFPTM
                     RKHNSDDDAALVTAEKDPRITKFGKLLRKTHLDELPQVINVLKGEMSFVGPRPERSEL
                     VQVFQSEVPFYRTRMLVKPGITGWAQIHQGYAETVEETSLKQEYDLYYISHATIWMDI
                     TILLRTVGSVLGLKGR"
BASE COUNT         1078 a         1002 c          939 g         1030 t
ORIGIN      
        1 cgtattcgtt ggccagggcg tagacgtgcg cgttggcgtt gatgttggca aggcagtatt
       61 cgtcggtgtc gcagtttggc ttggggtgat tgttggcgtg cttgtgggtg gcgtgcccag
      121 gacggtgaaa gtcgctgacc taacccaacc atgccccaca aaaaacagta cgcgatattc
      181 gcctggtttc cacttgctgg gttcaagttc cagcttcgcc acgccatagc ccccacttcc
      241 gccctgccag cgacccgtat gtgactggat gagatcacca tcacggtacc aaagttcagt
      301 ccactgcacg ccttcgtcca tcatgttgta actgtaacag gcataaatcg tttccagcgg
      361 gttctcaaat tctgttgccg ggtcaatcgg ctgcaagcca tcatcaattt cccgcgcaaa
      421 gagaatgcca ctcaaagcat aattatcacc tggggtaata atcgactcaa actgagacgc
      481 aatatcatct ggcacagacg gggtattcgt gaccgatggt gtgttggtga tggttggcgt
      541 aagcgtgatg gtgggcgtta gcgtaatcgt tggtgtcagg gtgatggtag gtgtcagtga
      601 tggggtcaaa ctgggcggaa aataggtgta cataacgcct tcaccatagc gaaaattcaa
      661 ataggcgaaa gcgaaaagca ctaaagcgcc gataatcaag cgaatgccca actcaatttg
      721 atgacgacgt ttggtgaaaa aagagagttt ccgaccttcg acaatcgttc ttcgggcacg
      781 ggacgctgtt gcaagccccg ccacgacagc caggatgctg atggtcatta tcgtcgagcg
      841 aatgtcgatg ttcatggtct gattatagca ttgctgccag ggtctggaaa agctgaaaaa
      901 atagcgttcg gctttaaaaa ttattcgttg agtgattctc tcgtggcttg cttgccacgc
      961 cggcgcataa tgtccagcaa acgtggcaaa acgttcgtgt aaagtttgta cagcaagggt
     1021 ctggcaggat aatcccaggc gccaatatgt cgcgcggttg tgccattgaa accttctttg
     1081 aatcggtaaa caccccaaag actgtcactt tcatcgaaga tatccggcgc gccccacata
     1141 tcgtaacttt cgcaacccag ctgtttggaa agttttatcg cttcccattg caaaaggtaa
     1201 tttggcatca actcccggta cttgtcggta gacataccat acatataccg tgccacaccg
     1261 ttaaagtgaa agataaacag tcccgccacc gcttcacctt caacttcggc gataagcgcg
     1321 tggcacatat cagcttccac aaaactttcc caaagctcca gatagtaatc ttctccgcgg
     1381 ataacaaaat catcgcgtaa ggcggtatac gcgtaagtct gataaagcat gggtaagtca
     1441 gccaaacttg cctggcgcac acttacacca cggcgcgtgg caagccgaat gttgtagcgt
     1501 gttttttgct tcatcgcggc aaggatttct tcttcatcac gcgtcaaatc gataaaaaca
     1561 gtgttacgaa attgaatctg gtcttgcgaa aacaaccagt tccgtccctt gagttcgttt
     1621 tcaaactcaa cacctaatgc tgatactgtt tcatcttctg cgccaggaac accggtacct
     1681 aaaagcacat cagggtcgat tttgagaaag atagctttct gatcgcgcgc gtagttctgc
     1741 aaatcgtcta aaacccgttc gcgcaaggct ttgttcgccc agtcaaggtt tgggcccttg
     1801 ggacaatata agacacgcag gcggagaaag ccaagtttga gttcccgtct caaaataagc
     1861 gcgcaggctt caatattttg agcttcgtca taccaaacga aggtttcggg cgtccagccg
     1921 ttgcggattt ttgacgcgcc ccattcccag gtctgtaaaa tatgcgcctc agggaactgc
     1981 aacaaaagct ggttccactc acttggcgtc gtcttaaagg cttgcatttt cgccacctgt
     2041 ccgcttattt ctaagccagc gataagcttt gtacaagagg ggtttaaaga ccttaacata
     2101 agctggcgcg gaacgcttca cttcaccacc aaagccgcgt ttgaagcgat aaacacccca
     2161 taagccgtcc gaacgtaaca taaattgttc ttcaagctcg tcttcatcag catcaggaat
     2221 gccccacata tcatacagtg tggcaccgtg ggccttggca taacgcatgg cttcccattg
     2281 caagagatag gtaggcatca gattacgatg cttgtcattt gacgcgccat aaaaatacca
     2341 ggcacgctgc ccatataaaa agagcatcaa ataggccagt gattcacctt catacgatgc
     2401 ccgcaaaagc acacactgac cttttggttc aaagatttca aacgcatttt ggtaataagg
     2461 cagactgtgt agcgcaaaac catcccgtcc gctggtgata agcatttgct cgtagaaatc
     2521 atccaggtcg tggctcgttt caaccttgat gcccttacgt tcagccaggc gaatgttata
     2581 gcgggttttt tgcttcatag ctgccagaat agccgtttct tccgaactga tatcaatcaa
     2641 aatcgtgcca cgtggttgta ttgttgcttc gtcttttatg taatttggaa agatactgtc
     2701 taaaccagca gtgtcataag gctcgtcaag gtcaggttca atttgcagga aaaccgcgtt
     2761 gcgctgacga cattcagttt caattttggg caacaatcgc gtccattctt caccaatgcc
     2821 cgctttcggc acataagcta ccgaaagtcc aagaggcaag cgcttgaaca gcaccagtgc
     2881 cgactcttgc ccgctatgta agcggactgg ttcccatcca tagcgcgcct taaattcgcc
     2941 ccagctatca gtttgcagaa tatgctggct ggcaggaaaa gcagaaaatg tcactttagc
     3001 gcccttttaa gcccaaaacc gagccgacag tacgcaaaag aattgtaatg tccatccaaa
     3061 tcgtggcatg ggaaatgtag taaaggtcgt actcttgctt gagagaagtt tcttcaacgg
     3121 tttcggcata gccttgatgt atctgcgccc agcccgtgat acccggtttg actaacatgc
     3181 gcgtccggta aaaaggcact tcgctctgga aaacctgtac cagctcactg cgttcaggtc
     3241 gtggccccac aaagctcatc tcgcctttca gaacattaat cacctggggc aactcatcta
     3301 agtgtgtctt gcgcagcaat ttaccaaact tggtaatgcg cggatctttt tctgcagtta
     3361 ccagggcagc atcatcatcc gaattatgct tgcgcatcgt tgggaattta tagattgtat
     3421 acggttttcc accccggcca aggcgttctt gcttaaagat aatgggtata tttccttcaa
     3481 ttaaaaggcc aagcgcaata accggaaaaa caacagctaa gccaataaga ccaagcccag
     3541 ctaaaaacaa gtccagtaaa cgtttaaaaa tacgataaaa accactgttg ggttttcgat
     3601 ccaaaaacga acgaatgacc caatcaggtt gcagtaagtc aatgggcaca cgtccggtga
     3661 tactctcata agcatcttgc atggtggtca gttcaagccc agcttcttgt gcgtccaaaa
     3721 tggtatggag catacctgga ttcatctcat gagtaatcgc caaaataaaa tcgctcacgc
     3781 caagtttttc ggtgatggat tccatcgtct tgtggtcgcc taaaacgggc acaccttcta
     3841 tgcgagtgtt tagtttaagg gggtcatcat caattaagcc aattagattg aaaggcttag
     3901 gatctagctt attgatttca cgaataagtt ctgtgcctgc attacccgcg ccaacaatta
     3961 aaacccgttt ttgttggttt accgctttga acacacggat aaaaataagt cgccaaatga
     4021 gcgttaatag cgcggtgacg atgataaaa
//
LOCUS       DAOSFC010000117         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_20208_length_4040_cov_3.641656, whole genome shotgun sequence.
ACCESSION   DAOSFC010000117 DAOSFC010000000
VERSION     DAOSFC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20208_length_4040_cov_3.641656"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /gene="purF"
                     /locus_tag="PK791_03920"
     CDS             complement(<1..1283)
                     /gene="purF"
                     /locus_tag="PK791_03920"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOH92494.1"
                     /translation="MSGIVGVVTPGSQRASNLLLYGLYALQHRGQVNTGMAMLDYNKV
                     VVKKGTGLINGVFPDDVPINLPGDRGLGHVKYGEYKNYDTHQPILPKEFTIRGVNCLI
                     SVDGNILNRDFNLYPLTLNLSGALVSAKSYLNTLHGAFAGVYLDRYKMIGFRDPLGLK
                     PICVGKLDDGYVIASETCALDSTGATFIKDLEPGEMVVIKDNQVEFHQYNQRLQKKKC
                     LFEYIYVARPDSYFDQVSIYNARSNMGRNLYHESPAEADIVMGAPDSGLIAAIGYAEA
                     SKIPYKDGIIKNRYIGRTFINPDENTRTKDIFIKLNPIKEVLRDKRVVLVDDSIVRGS
                     TSKRTIEMLKEAGAKEVHVRIAAPMVRHSCDLALDMPDSSKLVAHDRDAEEIRAYINA
                     DSLHFLSLEGLKDACQGTDYCDRCFTGVYPIQEIL"
     gene            complement(1284..1763)
                     /gene="purE"
                     /locus_tag="PK791_03925"
     CDS             complement(1284..1763)
                     /gene="purE"
                     /locus_tag="PK791_03925"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOH92495.1"
                     /translation="MMKVALIMGSKSDLPVALKAMEMLDKFGVPYSARVISAHRALDM
                     LTKCVNSFEKDAVKVVIALAGKAAHLPGVIAGMTTLPVIGVPIKGSAFLGMDSLLSIV
                     QMPTGVPVATVAVDAADNAAILACQILALNSPELKQKLLKYKRELAKDVAEQDRSIQ"
     gene            complement(1769..>1965)
                     /locus_tag="PK791_03930"
     CDS             complement(1769..>1965)
                     /locus_tag="PK791_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92496.1"
                     /translation="KVDIIAMNTVSHLPSDCGVDSIASKCGHVFGSRALIERMGPGLY
                     QNLAISNLPPHFAQLAKSFS"
     assembly_gap    1966..2065
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2066..>4040)
                     /locus_tag="PK791_03935"
     CDS             complement(<2066..>4040)
                     /locus_tag="PK791_03935"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOH92497.1"
                     /translation="KIIRLFKRPEVTRLIKKCNDFGAGGVSVAIGELADGLDINLNAV
                     HTKYPGLNGYEIALSESQERMAVVIAPKDLVAFIGYCDEEGLEYAQVARVSEEPRMRM
                     FWHGEAIVDLSRALLNSNGATKHATVVLNTHHQETPEQKSNTRLNRSLGKSLSQNFDA
                     SLGRDTVLAEYGGKHGLTAQDGIVTRFPAEGTNTASVMTYAFFPGIAKASAYHGGYYA
                     VLQSIVKTIALTGQYADIRLSLQEFFPSIKGNPERMGLPFLALLGAFEVMKALDIPAI
                     GGKDSMSGSWHEIDVPPSLVSFAVNTADSQKVVSREFKQANSRILLTKIACDAQGMID
                     FEQFARVMEAFGALHQDGKVLAASAVSEHGIRFTLDDMALGNRLGLEIKPELLDTFIP
                     GSIIFEVSSDLNYDPSLFEEIGCTVENGEHQALVQTRQADYTSIYGDILESKPVEIPA
                     FETRPLEPIKLNNKKVLIPVLDGATGEYDLQRAFESAGFEVCQEIIHTDSFEAYQNSL
                     SQLANAVQNAGVFAIPHGDYFGSVAGNVAGAMTQLLKHPTIFRALQALRERQGFIFGV
                     GAGMATLVDAGYFGDIQNDFIFTSNKNNAYTHFMQDISIIKNSYFSNADITHYTAPIS
                     GRQMTIRCNDLEQLAKKVDIIAMNTVSHLPSDCG"
BASE COUNT          910 a          979 c         1057 g          994 t
ORIGIN      
        1 cataatatct cctgaatggg ataaacaccc gtaaaacaac ggtcgcaata gtcagtgccc
       61 tggcaggcgt ccttgagacc ttccagcgac aggaagtgca gggaatctgc attgatatag
      121 gcccggattt cttcggcatc gcggtcatgg gcgacgagtt tggatgagtc tggcatatcc
      181 agcgcaaggt cgcagctatg cctcaccatc ggcgcagcaa tgcgcacgtg cacctctttc
      241 gcgccagctt ctttaagcat ctcaatggtg cgctttgaag ttgaaccacg cacaatggag
      301 tcatctacca aaacaacccg tttgtccctt aagacttcct tgatggggtt gagtttgata
      361 aaaatatctt tggtgcgtgt gttttcgtcg ggattgataa aggtcctgcc gatgtagcgg
      421 tttttgatga taccgtcttt atatgggatt ttggatgctt cggcgtaacc gatggcggca
      481 atcaagccac tgtcgggcgc gcccatcacg atatcagctt cggcagggct ttcgtggtaa
      541 aggttgcgcc ccatgttgct gcgcgcgtta taaatggaga cctggtcaaa ataactgtcg
      601 ggtcgcgcca cataaatgta ttcaaaaagg cattttttct tttgcaggcg ttggttgtac
      661 tgatggaatt cgacttgatt gtctttgatg acgaccatct caccgggttc aaggtctttg
      721 atgaatgtcg caccggttga gtcgagcgcg caggtttcgg aggcgataac atagccatca
      781 tctagtttgc cgacgcagat gggtttgagc cccagcgggt cgcgaaaacc gatcatctta
      841 taacggtcca gataaacgcc ggcaaaggcg ccatgcaggg tgttgagata agatttcgcg
      901 cttaccaaag cgccactcag gttgagcgtc agcgggtaga gattgaagtc gcggttgaga
      961 atattgccat caactgaaat aagacagttc acgcccctga tcgtaaactc tttgggcagg
     1021 atgggctggt gggtatcgta gttcttatac tcgccatact tcacatggcc cagacctctg
     1081 tcgccgggca ggttgatggg aacatcatca gggaaaacgc catttatcag tccggtgccc
     1141 tttttaacca caaccttgtt gtagtcgagc atggccatgc cggtattaac ttgtccgcga
     1201 tgttgcaggg cgtaaagccc ataaagcaaa agattcgacg ccctttgact gccgggagta
     1261 acgacgccta cgataccact catttactga atactcctgt cttgttcggc aacgtctttg
     1321 gcaagttcac gtttatattt taacagtttt tgtttcaatt ccggtgaatt gagcgccaga
     1381 atttggcatg ccaggatggc ggcgttatcg gcagcatcta ccgccacggt tgccacggga
     1441 acgccggtgg gcatctgcac gatggagagc aaagagtcca tacccaggaa agccgagcct
     1501 ttgatgggca cgccgataac cggcagggtg gtcatgccgg caatgacgcc cggcaaatga
     1561 gcggcttttc cagccagggc gatgacgacc ttcaccgcat ctttttcaaa actattgaca
     1621 cacttggtga gcatatccaa agcgcgatgg gcggaaatca cccgcgcgct gtaaggaacg
     1681 ccaaatttat ccagcatctc catcgctttt agggcgactg gcaaatctga tttacttccc
     1741 attatcaagg caactttcat catttctcct aactaaaact tttcgccaac tgggcaaaat
     1801 gcgggggcaa attgctgatg gcaaggtttt gataaagtcc ggggcccatg cgttcaatca
     1861 gcgcgcgcga gccaaaaaca tgcccacatt ttgaggcgat gctgtcaaca ccacagtctg
     1921 atggcagatg tgaaacggtg ttcatagcaa tgatatcaac tttttnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnccaca gtctgatggc agatgtgaaa cggtgttcat
     2101 agcaatgata tcaacttttt tcgcgagctg ttccaagtca ttgcagcgga tagtcatttg
     2161 cctgccacta atcggcgcgg tgtagtgggt gatatccgcg ttgctaaagt agctgttttt
     2221 gatgatgctg atatcctgca tgaagtgggt gtaagcattg tttttgttgc tggtaaagat
     2281 aaagtcgttt tggatatcgc caaagtatcc cgcgtccacc agagttgcca tgcccgcccc
     2341 gacgccaaag ataaagccct gacgttcgcg aagcgcttgc aaggcgcgga aaatcgtggg
     2401 gtgctttagc agctgggtca tcgcgccagc gacgttgcca gccacagagc cgaaataatc
     2461 gccatgggga atggcaaaga cgcctgcgtt ttggacggcg tttgccagct ggcttaggct
     2521 gttctggtaa gcctcaaaac tgtcggtatg gataatttcc tgacacactt caaaaccagc
     2581 actttcgaaa gcgcgttgca ggtcatattc gcctgtggca ccatcgagga cgggaatcag
     2641 cactttttta ttgtttagtt taatgggttc aagcgggcgg gtttcaaagg cgggaatctc
     2701 gactggcttt gattccaaga tatccccgta gattgacgtg taatctgcct ggcgtgtttg
     2761 cacgagtgct tgatgttcgc cattttcaac ggtgcatccg atttcttcaa acaggcttgg
     2821 gtcataattc aaatctgagc tgacttcaaa aatgatgctg ccggggataa acgtatccag
     2881 cagttcgggc ttgatttcta aaccgagccg gttacccaaa gccatgtcat ctaacgtaaa
     2941 acgaatgcca tgttcgctaa ccgctgaagc cgccaaaacc ttgccatcct ggtgtaaagc
     3001 accaaaagct tccatgacgc gggcgaattg ttcaaaatca atcataccct gcgcgtcaca
     3061 ggcgattttg gtaaggagaa tccgcgaatt cgcttgtttg aactcgcgtg agacgacttt
     3121 ttgactgtcc gcggtgttga cggcgaagct aaccaggctg ggcgggacgt caatctcatg
     3181 ccaggagcca ctcattgagt ccttgccacc gatggcaggg atatcgagcg ctttcatcac
     3241 ttcaaacgcg cccaagagcg ccaggaaggg caagcccatg cgttccgggt tgcctttgat
     3301 gctggggaaa aattcctgca aactcaggcg gatgtcggca tactggcccg tcagcgcgat
     3361 ggtttttact atggattgca aaacggcgta ataaccaccg tgatacgcgc tggcttttgc
     3421 gatgcccggg aagaaggcgt aagtcatcac cgacgcggtg tttgtgcctt ccgcggggaa
     3481 gcgggtgacg atgccatcct gggcggttaa gccatgtttg ccaccatact ctgccagcac
     3541 ggtgtcgcgc ccgagtgacg cgtcaaagtt ttgcgacaag gatttaccca aactgcgatt
     3601 gagtcgggtg ttgcttttct gttcaggggt ttcctgatga tgggtgttaa gaacgacagt
     3661 ggcgtgtttc gtcgcgccgt tggagttgag caaagcgcgg ctcaggtcaa caatcgcttc
     3721 accatgccag aacatacgca tgcggggttc ttcgcttacc cgcgcgacct gggcgtattc
     3781 cagtccttct tcgtcgcagt agccgatgaa tgccaccagg tcttttgggg cgatgaccac
     3841 cgccatgcgt tcctgtgact ctgagagcgc gatttcgtaa ccgttcaggc ctgggtattt
     3901 ggtgtgcacc gcgttcaggt taatgtctag cccatcagcc agttcgccga tggccaccga
     3961 tacacccccc gcgccaaagt cattgcattt tttaattaga cgggtgactt cagggcgttt
     4021 gaacaggcgg ataatcttgc
//
LOCUS       DAOSFC010000118         4035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_20242_length_4035_cov_5.374874, whole genome shotgun sequence.
ACCESSION   DAOSFC010000118 DAOSFC010000000
VERSION     DAOSFC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20242_length_4035_cov_5.374874"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2329)
                     /gene="alaS"
                     /locus_tag="PK791_03940"
     CDS             complement(<1..2329)
                     /gene="alaS"
                     /locus_tag="PK791_03940"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOH92498.1"
                     /translation="MFLTGNQIRQSFLDFFARHGHRVVPSASLVPGGDSTLLFTNAGM
                     VQFKDVFLGTANAPAPRVANSQKCMRVSGKHNDLDTVGRDDTHHTFFEMLGNWSFGDY
                     YKKEAIAWSWELLVNEWGLDPAKLYASYFVDEEGIIPDDNEARDYWLQQPGFNPAHLV
                     KGNRHDNFWEMAETGPCGPCTEIHYDFGPEACDKQGVEGHVCEVNGDCNRIVEIWNNV
                     FIQFNRLSATDFQPLPKTHVDTGMGLERIVAVLQGVKGNYETDLLKPLLDETQRIAGQ
                     SDQERAENLTAYRVIADHTRAASFLIADGVIPGNVGRNYVCRMIIRRAARFARTIGLK
                     EPFMARVAKIVVEIYGQHYPELKREQELIYESFTREESRFAETLDTGFAELNLHMQAL
                     KDAGKTVLGGYEAFDLYATLGFPLEITRDILSESGFSVDEDGFYAAMEEHRLASGKGQ
                     AGIENDSQKAELYQSLDKQLHDDGFLGSEGVIQDPYTSLSQTSQLLAIIKDGQIVESV
                     SQGDEVELVIQHTPFYLESGGQVSDTGKIVSSAGDWEVAIDNVIKPSAGLIVHLGKVL
                     HGTPQLQDKIVVSVDLPRRRAIMRNHTGTHLLHTALRKVLGEKVHQAGSAVDAERLRF
                     DFNYPQAVAKADLLEIEALVNSYIADEYPVIKQSKTLEQAQKDGAMALFGEKYGAEVR
                     TIEIKDERGRVSYELCGGTHVENTAEIGSFYITSESSVAAGMRRIEAVTGMGAYEYAR
                     KRLDLQNELSAHLQSSPSEALAKVQNLEDSLKASQA"
     gene            complement(2380..2988)
                     /gene="recR"
                     /locus_tag="PK791_03945"
     CDS             complement(2380..2988)
                     /gene="recR"
                     /locus_tag="PK791_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOH92499.1"
                     /translation="MKALPLPVQSLIDAFSRLPGIGPKTAARLAFYLLKDEGDTAQAL
                     ADALLNLKADTSYCEQCHNITLAGQALCEVCADTRRDEHLICVVEEPSDVIAIERTGI
                     FNGKYHVLLGVLSPIEGIGPEQIKLQSLLERLQKNAVSEVIIATNPTMEGDATALYLQ
                     KRIAPLGVSVSRLARGLPVGGDIEYADQNTLQRALAGRQSME"
     gene            complement(3026..3367)
                     /locus_tag="PK791_03950"
     CDS             complement(3026..3367)
                     /locus_tag="PK791_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOH92500.1"
                     /translation="MAKGYRGPAGRGGGNMMAQIAKLQEQMEQAQAELAEKTVTETAG
                     GGAVQVTMTGDQHCREIIIDEELLADGDVELLQDMVLTAVNKALDASRALSEEQMAPF
                     TNMLSGFGMGV"
     gene            complement(3368..>4035)
                     /locus_tag="PK791_03955"
     CDS             complement(3368..>4035)
                     /locus_tag="PK791_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92501.1"
                     /translation="DQKTNWHPGLGLELAYASAISWQESAPVPQVQSIPQASYSAPAA
                     SYSSPASQIAQSPTVKSSPKVSPTPKPAQSKKPAPAKKTPPAQVQDMPNQTGAAEGKM
                     ELTSENLNAEMDKFKTAIKRGNSSTVGLINSARIYVPNNKTLVLNFATQILCDMMRDE
                     NNMKVMRIVVEYFFGKDIRIICNFGDYDYTEHANDSDEEESLLDAVAKRFNGVIIETG
                     KED"
BASE COUNT          849 a          987 c         1108 g         1091 t
ORIGIN      
        1 cagcctgact ggcttttagg gagtcttcca ggttttgaac cttggcaagg gcttcacttg
       61 ggctgctttg caaatgcgcg gaaagctcgt tttgcaggtc aaggcgtttg cgggcgtatt
      121 cataagcacc catgccggtt acggcttcaa tacggcgcat tccagctgcg acgctcgatt
      181 cgctggtaat gtagaaagaa ccgatctcgg cggtgttctc aacgtgggta ccaccgcaaa
      241 gttcgtaaga aactctgcca cgctcatctt tgatttcaat cgtgcgcact tccgcgccgt
      301 atttctcgcc aaagagggcc atcgcgccgt ctttttgggc ttgctcaagc gttttgcttt
      361 gtttgatgac cggatattcg tccgcgatgt agctgttgac cagggcttca atctccagca
      421 aatccgcttt ggcaacggcc tggggatagt tgaagtcgaa gcgcaatcgc tccgcgtcaa
      481 cagccgaacc ggcctggtgc actttttcgc ctaaaacttt gcgcaaggcg gtgtgcaaaa
      541 ggtgtgtgcc ggtatggttg cgcataatcg cgcgtcggcg tggcaagtcc acactgacga
      601 caatcttatc ttgtagctgg ggtgtgccat gaaggacttt gccaagatgc acaatcaagc
      661 ccgcggaggg tttgatgacg ttgtcgatgg cgacttccca gtcaccagct gaggaaacga
      721 ttttacccgt gtcgctgact tgtccaccgg attccaggta aaagggtgta tgttggatga
      781 ccagttccac ttcatcaccc tgggagacgc tttcaacgat ttgtccgtct ttgatgattg
      841 ccaaaagctg gctggtctgg ctaaggctgg tgtaggggtc ttgaattacg ccttcactgc
      901 ccaaaaagcc atcatcgtgg agttgtttgt ccagggactg gtaaagttca gccttttggc
      961 tgtcgttttc gatgccagcc tggcctttgc cggaggcaag acggtgctct tccatggcgg
     1021 cataaaaacc gtcttcatca actgaaaagc ccgattcgct taagatatcg cgggtgattt
     1081 ccagggggaa accgagtgtg gcgtaaaggt caaaggcttc atatccgcca agcacagttt
     1141 tgcccgcgtc ttttagcgct tgcatgtgca ggttgagttc agcaaagccg gtatcgagtg
     1201 tttcagcaaa acgggactct tcacgggtga agctttcata gataagttcc tgttcgcgtt
     1261 tgagttccgg gtaatgttgc ccgtaaatct caacaacgat tttggcaaca cgtgccataa
     1321 agggttcttt taagccgatt gtacgggcga aacgcgccgc gcgacggata atcatgcggc
     1381 agacgtagtt gcgtccgacg ttgccaggga tgaccccatc cgcgattaaa aagcttgccg
     1441 cgcgggtgtg gtccgcgata acacgatagg cggtgaggtt ttcagcgcgt tcctggtcac
     1501 tttgcccggc gatgcgttgg gtttcgtcga gcaggggttt gagtaaatcg gtttcgtaat
     1561 ttcccttgac gccttggagc acagcgacaa tgcgttccag acccatgccg gtatcaacgt
     1621 gcgttttagg cagcggttgg aaatcagttg ctgacaggcg attgaactgg ataaaaacgt
     1681 tgttccaaat ttctacaatg cggttgcaat caccgtttac ttcgcaaaca tggccttcaa
     1741 cgccttgttt gtcgcaggct tcggggccaa agtcataatg gatttcggtg caggggccac
     1801 atggaccggt ctcggccatt tcccagaaat tatcatggcg gttgccttta acgagatgtg
     1861 caggattgaa accgggctgc tgaagccagt aatcgcgggc ttcattgtca tcagggataa
     1921 tgccttcttc atcaacgaag tagctggcat agagtttggc agggtcaagt ccccactcat
     1981 ttaccagcag ttcccagctc caggcgatag cttctttttt gtaataatcg ccaaatgacc
     2041 agttgccaag catctcaaaa aaggtgtgat gggtgtcatc acgaccaaca gtatccaggt
     2101 cgttatgttt tccggaaaca cgcatgcact tttgcgagtt tgccacacgt ggggcgggtg
     2161 cgttggcggt gcctaaaaag acatccttaa actgcaccat accagcgttg gtaaacagca
     2221 gggttgaatc cccacccggt acaagtgacg cactgggcac aacacggtgg ccgtgcctgg
     2281 cgaaaaagtc cagaaatgat tgtctgattt gatttccagt taaaaacata tacaactcca
     2341 gttaaggtct taggcggaaa ttatactctg aaaagcgaat tattccatgc tttgacgccc
     2401 agccagggca cgttgtagcg tgttttggtc ggcgtattca atatcaccgc caacgggcag
     2461 tccgcgcgcc aggcgactga cgctcacacc caggggggcg atgcgctttt gcaggtagag
     2521 cgcggtggcg tcgccttcca tggttgggtt ggtggcgatg atgacttcgc tcaccgcatt
     2581 tttctgaagg cgttcaagca aggactgaag tttgatttgc tcggggccta taccttcgat
     2641 gggcgaaaga acaccgagta aaacatgata ttttccgtta aaaatgcccg tgcgttcaat
     2701 ggcgatgaca tcagacggtt cttcgacaac gcaaatcaga tgctcgtcgc gtcgcgtgtc
     2761 ggcacagact tcgcataagg cttgccctgc cagggtgatg ttatggcatt gttcgcaata
     2821 gctcgtgtcg gctttgagat ttagcagcgc gtctgcaagg gcttgggctg tgtcgccttc
     2881 atctttgaga agataaaaag ccagcctggc ggcggtttta ggcccgatgc caggcaggcg
     2941 cgaaaaagcg tcaattaaac tctgaactgg tagcggaagg gctttcattg tgtgctaaaa
     3001 atgcctttta ataaagatca aaggcttaaa cgcccatgcc gaagccggaa agcatgttgg
     3061 tgaagggtgc catttgctct tcagacaggg cgcgtgaggc gtccagtgcc ttattaaccg
     3121 cggtgagcac catatcctgc aagagttcaa cgtcgccatc agcgagaagt tcttcgtcaa
     3181 tgatgatttc cctgcaatgc tggtcgccgg tcatggtcac ttgcactgcg ccaccgcccg
     3241 cggtttcggt cacggttttt tcggcgagtt cggcttgcgc ctgttccatc tgttcctgca
     3301 gtttggcgat ttgcgccatc atgtttccac cacctctgcc ggcgggtcct ctgtatcctt
     3361 ttgccattta gtcctctttt cctgtttcta taatcacgcc attaaaccgt ttggcgacgg
     3421 cgtcgagtaa actttcttct tcgtcgctgt cgttggcgtg ttctgtgtaa tcataatcac
     3481 caaagttaca aataatgcgg atgtctttgc caaaaaagta ttccaccact attctcatca
     3541 ctttcatgtt gttttcatct ctcatcatat cgcacaaaat ttgcgttgca aagtttagga
     3601 caagtgtttt gttgtttggc acatagatgc gcgcggaatt gatgagtcca acggttgaag
     3661 agttgccacg tttaatggca gttttgaact tgtccatttc tgcgttcagg ttctctgatg
     3721 tgagttccat ttttccttca gcagccccgg tttggtttgg catatcttgt acctgggcgg
     3781 gcggggtctt ttttgccggc gcaggttttt tgctttgggc gggtttgggt gtcggtgaaa
     3841 ctttggggct ggatttgacc gttgggcttt gcgcgatttg cgacgctggg ctgctatagc
     3901 ttgcggcggg cgcgctatag ctcgcttgtg ggatgctttg tacctggggt accggcgctg
     3961 attcctgcca ggaaatcgcg ctggcgtatg ccagttcaag cccaaggccg gggtgccagt
     4021 tggttttttg gtccg
//
LOCUS       DAOSFC010000119         4022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_20340_length_4022_cov_4.197378, whole genome shotgun sequence.
ACCESSION   DAOSFC010000119 DAOSFC010000000
VERSION     DAOSFC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20340_length_4022_cov_4.197378"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PK791_03960"
     CDS             complement(<1..925)
                     /locus_tag="PK791_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOH92502.1"
                     /translation="MMSIQKVSRLVICLAITLSLVACGFGTNQEPTLAPPQVSIETPF
                     PNGDPTPLPTYTPTMAALGSPDNPITIGIINPTPETEQTDGLAQLASQLSGALQLSVE
                     GKIYQDYMSLELALQKNELHFAWLQTPEYLLATQKDLVSSILGVNNLGVSAYGIQYLA
                     HRDSGLKEYFDLGSNTATSSPEHALQQFAGMRPCLTNSKSLAGYWVPLAYLAQSNISW
                     EPPVETQSYNASLRALYIQGICAFTSTYAISADPRSSSAVITDLPDVIEKLPVIWISP
                     PIIPNRALAASNQVDLSLQTRVSEFLLNYARN"
     gene            complement(975..1574)
                     /gene="rdgB"
                     /locus_tag="PK791_03965"
     CDS             complement(975..1574)
                     /gene="rdgB"
                     /locus_tag="PK791_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOH92503.1"
                     /translation="MKKLLIASNNAHKFQELSALLADLNLKLIKPSDLGIKIDVAETG
                     DSYYANALIKARAFHEASNKPVLADDSGLEVNALNGLPGIHSHRLVPIKHATDRDRCF
                     YLLGRLAETPKPWLAAFHSTTILYINSQEIYSSHGICPGQIIEDFRGENGFGFDPIFL
                     LADRNQTMAELSEKQKNELSHRARAIQAFDALRNWANRS"
     gene            1657..2142
                     /locus_tag="PK791_03970"
     CDS             1657..2142
                     /locus_tag="PK791_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOH92504.1"
                     /translation="MEKKIYSLRTNVGYFYNRPIGYSRSFHQEYDEIFIQPDLNLRDL
                     IVDYTFSRTREGLLLQAQLSTHMEAQCARCLTPFYANIKSEFEELYTFPERFQQETDD
                     ETIPEDGYIDLGVLFRDYLLIELPMNSVCKPDCKGICIECGQLLNEGDCEHTDSRIIF
                     D"
     gene            2206..4005
                     /gene="metG"
                     /locus_tag="PK791_03975"
     CDS             2206..4005
                     /gene="metG"
                     /locus_tag="PK791_03975"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOH92505.1"
                     /translation="MNENILIAIAWPYANADIHVGNITGSHLPGDIVARYHRLRGRNV
                     LMVSGADAHGTPITIKADQLGKTPMQVYESYQPRFVELFERLGISYDLFTSTHTENHF
                     EIAQKVFVTLLENGYLHTEISKQWFSEKQQRFLPDRYVVGTCYLCGAERQRSDQCELC
                     GQILDPEKLIDPISIIDESTPVLKETEHYYLDLRSLEPKIRDFLLEREDYWRPNVMRQ
                     SLGNIESVGLKPTSITRDLQWGIPVPLKGWENKVLYVWFEAVMGYLSATIEWSSLHEK
                     PNAWRDWWTNPDAKAFYFIGKDNITFHAVTWPAELLGMDTGFDAHMGAKIPQALNLPY
                     NVPANEFMNLEGRKISGSANWAVWGLDILDRYDADAIRYYLTVTMPETRDTDWDWEDF
                     FNRNNSELLATWGNLANRVLSFTYKNWVGVIPAPEELRDSDKELLQKISDGFDAVAEK
                     IEKVELRNALGQIMDLATEVNKYLDVHAPWFEIKTDRAQAEKSVFTAIQAIEWLNVMF
                     APFLPHTSEALHKILNHPGTLFGVSKQKEVKDELGQHLTLGYELQEDCLSKPGQDLWQ
                     AKEMEGGREFNKPTPLIKKLDASIIAEERARLG"
BASE COUNT         1101 a          803 c          992 g         1126 t
ORIGIN      
        1 cattgcgcgc gtagttaagc aggaattctg atacgcgcgt ttgcagggaa aggtctacct
       61 ggtttgaagc cgctaaggca cgatttggga tgatgggcgg tgaaatccaa ataaccggca
      121 gtttttcgat gacgtcgggt aaatcggtga tgaccgctga agaactgcgc gggtcggcgc
      181 tgatggcata ggtcgaagta aaagcgcaga tgccctggat gtatagcgcg cgtaaactgg
      241 cattgtagct ttgggtttca acgggtggtt cccagctgat attgctttgg gctaaatatg
      301 ccaggggtac ccagtaaccc gccagggatt tgctgtttgt cagacatggt cgcatgccag
      361 caaattgttg caaagcatgt tctggactgc tcgttgcggt gttactgcca agatcgaaat
      421 actctttaag tcctgaatca cgatgtgcca agtattggat tccgtatgca cttacaccga
      481 ggttgtttac gccgagaatt gaacttacca ggtctttttg ggtagccaaa aggtattcag
      541 gcgtctgaag ccaggcgaaa tgcagttcgt tcttctgcaa ggcaagctcc agtgacatat
      601 agtcctggta aatctttcct tcaacactta gttgcaaagc gccagataac tgactggcaa
      661 gttgagcaag accgtcagtt tgttcggttt cgggagtagg gttaataatg cctatggtga
      721 ttgggttatc agggctgcca agtgctgcca tggttggggt gtaggtgggc aaaggtgttg
      781 ggtcaccgtt tgggaaaggc gtttcgatgc tgacttgagg tggggctaag gtaggttcct
      841 gattagtgcc aaatccacaa gccactaaac ttagtgtaat ggccagacat atgaccagcc
      901 ttgacacctt ttgaatagac atcataatga aattttacct tattttttaa aaaccatttt
      961 ctcttgcaat ggttttaaga cctatttgcc cagtttcgta aggcatcaaa tgcttgaatg
     1021 gctctcgcgc ggtggctaag ctcatttttt tgtttttccg aaagttctgc catggtctga
     1081 tttcggtcgg ccaacaggaa aatggggtca aagccaaagc cattttctcc gcgaaaatct
     1141 tcgattattt gtccagggca gataccatga gaactgtata tctcctgaga attgatgtag
     1201 agtatcgttg ttgaatgaaa agcagccagc cagggcttgg gcgtttcagc cagtctgccc
     1261 aaaaggtaaa aacaacggtc acgatcagtg gcgtgcttaa ttgggactaa gcggtgggag
     1321 tgaatccctg gtaatccatt taatgcgttg acttcaagcc cggaatcgtc tgccaggacg
     1381 ggcttattac tggcttcgtg aaatgctcgc gctttgatta aggcgttggc atagtagcta
     1441 tcgcctgttt cggcgacatc aattttgatg cctagatcgc ttggtttgat gagtttcagg
     1501 tttaaatctg ctaaaagcgc agaaagttct tgaaacttat gggcattatt gctggcgatg
     1561 agtaattttt tcataatagc tgtcccagaa tccttttata tttacggctt aggatggcat
     1621 gatataattt agaaaaccta agagattgta ctcgttatgg aaaagaaaat ctattcacta
     1681 cgcacaaatg ttggctattt ctacaaccgg cctattggtt acagtcgctc ttttcatcag
     1741 gaatatgatg agatattcat tcaacctgat ctgaatttaa gagatttgat tgttgattac
     1801 acgttttcac gcacacgtga aggcttgctg ttacaagcgc agttaagcac acatatggaa
     1861 gcacaatgcg cgcgatgtct aacccctttt tatgcgaata taaagtcaga atttgaagaa
     1921 ctttatacct ttcccgaacg ctttcagcag gaaacggatg atgaaacgat tcctgaagat
     1981 ggctatattg atttaggcgt actgttcagg gattatttgc tcattgaact gccgatgaac
     2041 agtgtctgca agcctgattg caaaggaatt tgtattgagt gtgggcaatt attgaatgaa
     2101 ggggattgtg aacacactga tagccgcatc atttttgatt aaatttacaa tatcgaagca
     2161 cgttatggtg ataaaatttt gaaacacatt taagtggagt taactatgaa tgaaaatata
     2221 ttaattgcaa ttgcatggcc atacgctaac gcggatatcc acgtcggcaa tattactggc
     2281 tcacatcttc ctggggatat tgtcgcccgt tatcatcgtt tgcgcgggcg caatgtactg
     2341 atggtctcag gtgctgatgc tcacggaaca cccattacca tcaaagctga ccaacttggt
     2401 aagaccccta tgcaggttta cgaaagctat caaccgcgtt tcgtcgaatt gtttgaacgt
     2461 ctggggatca gttatgactt gtttacttca acgcatacag agaatcattt tgaaattgct
     2521 caaaaggttt tcgtaacact gcttgaaaat ggttatttac acaccgaaat ttcaaagcag
     2581 tggtttagcg aaaagcaaca acgattctta cccgaccgct atgtggtggg tacgtgttat
     2641 ctttgtggcg cggaaagaca aaggtcagat caatgtgaac tgtgtggaca aatattagac
     2701 ccggaaaaac ttattgatcc gatttcaatt attgacgaat caacgccggt gctaaaggaa
     2761 accgaacatt attatctcga tttgcgttca ctcgaaccaa aaatccgcga ttttctgctt
     2821 gaacgcgaag actactggcg accgaacgtg atgcgccaga gtttaggtaa tatcgaatcg
     2881 gtaggcttga aaccaacatc aattacaagg gacttgcagt ggggtattcc cgtgccactt
     2941 aaaggctggg aaaacaaggt gctttatgtt tggtttgaag ccgttatggg atatctttcc
     3001 gcgacgattg aatggtcaag tctgcatgaa aaaccgaatg cctggcgtga ctggtggact
     3061 aatcctgacg cgaaggcttt ttacttcatt ggtaaggata atattacctt ccatgcggta
     3121 acctggccgg cagaattgct gggtatggat actggttttg atgcccatat gggagcaaaa
     3181 atcccgcaag ctcttaattt gccttacaat gtgcctgcca acgagtttat gaatctggaa
     3241 ggacgcaaaa ttagcggttc agctaactgg gcggtttggg gactggatat actcgatcgt
     3301 tatgacgccg atgctattcg ctattatctg accgtgacca tgcccgaaac ccgtgatacg
     3361 gactgggact gggaagattt cttcaatcgc aataacagtg aactcttagc gacgtggggc
     3421 aacctggcaa atcgcgtgtt gagttttact tataaaaact gggttggtgt tattcctgca
     3481 cctgaagaac tgcgtgattc agataaggaa ttactgcaaa agatttcaga tggttttgat
     3541 gccgttgctg aaaagattga aaaagtagaa cttcgaaacg ccttaggaca aattatggac
     3601 ttggctactg aagtcaataa atatcttgat gtgcatgcgc cctggtttga aattaaaacc
     3661 gaccgcgcgc aagctgaaaa atctgtcttt acagctatcc aggctattga atggctgaat
     3721 gtcatgtttg cacctttcct gccacacaca tccgaagcgc tacataaaat attgaaccat
     3781 cctggaacgc tttttggtgt cagcaagcaa aaagaagtca aagatgagct tggccaacac
     3841 ctgacgctag gctacgaatt gcaagaagat tgcctcagca agcctggaca agatttgtgg
     3901 caagcaaaag aaatggaagg cgggcgagag ttcaacaagc ctacaccatt gatcaaaaag
     3961 ctggacgcca gcattatcgc ggaagaacgc gcgcgattgg gttaggtgtt ttgtaggata
     4021 aa
//
LOCUS       DAOSFC010000120         3954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_20873_length_3954_cov_5.752501, whole genome shotgun sequence.
ACCESSION   DAOSFC010000120 DAOSFC010000000
VERSION     DAOSFC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20873_length_3954_cov_5.752501"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PK791_03980"
     CDS             complement(<1..426)
                     /locus_tag="PK791_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92506.1"
                     /translation="MKTYQFDPEKLKKQTKILALMYSIPLVLMVVLVFFLYRDRPEMQ
                     NQAIIMVVLMVVFYGFMGFRRHKQNVQLWKEYSLTVDDEGVSQTQPNFPKVRVPKEEV
                     KSIEVVKNGFYVQTYSQGRVLGISKELADADYEELKQIFS"
     gene            complement(607..999)
                     /locus_tag="PK791_03985"
     CDS             complement(607..999)
                     /locus_tag="PK791_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HOH92507.1"
                     /translation="MKRYRTAAILQIIHGGLMEIGGVLFLISALTFGFSNSNAGQYFE
                     FKLQYFQDNLYMMSVMSLIYGILRIIGAVGLLKNRMWGLVLSVINSVTTMILMMFLLP
                     AGIMDGILAVTALVLILVEYYGDKKISG"
     gene            complement(1055..2251)
                     /locus_tag="PK791_03990"
     CDS             complement(1055..2251)
                     /locus_tag="PK791_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HOH92508.1"
                     /translation="MKNKRNKSMIKTILLSILSLAFSLLLLAGFIWKNEIRTVASIKE
                     IMPGDDDTNGYVYEMTVFGDYYLDEYLEAGGTKNDKELISFLTSKITKGLFNLSIEEA
                     DIVGCSSFSANATNGDKIFARNYDMYKTNVAIVHTNPANRYKSISTVDLSFLGVPSDK
                     KMEGLRNKINTIAAAYAPLDGMNEKGLTVGIYMTHQGPGKINVPTNQENGRPDITSTV
                     LLRVILDYAATVEEAVAIAEKYDMHDSANSSFHYMISDANGKSAVLEYIAGTDSTDTD
                     GQLRELMVIYNDDPMSLAGTDKYQLVTNFIVYPGYYEEGDKQHGLDRYRTVKEELEKR
                     GGLVKDDEDAMIVLEKVALRNLDNDIYSVTIHSVIYNQSNKTVYWVGNRHYGEKAYIY
                     KFNLME"
     gene            2588..>3954
                     /locus_tag="PK791_03995"
     CDS             2588..>3954
                     /locus_tag="PK791_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOH92509.1"
                     /translation="MKEFSTASIRNIVLASHSNSGKTMLIENMLNFTGATTRIGKIED
                     GTTTSDWDEEEQKRQISLFSSLVPILYKDIKFNFIDTPGYSDFVGEVVSAMRVADAAL
                     ILVDSVSGAEVGTETALYYAEKFGMPRFLLLNKMDRDNASFAKAMKSVEEVTDARLIP
                     MHLPIGEKADFKGVVDLLTMKAYLGDGKTASDIPAEMQAEAEEARTVLVEAAAEGEDA
                     LLEKYFENGDLTPEEMVQGLHKAIQVSNIIPVMVAAGGRMMGIGPILEGLLALAPSPL
                     ERPAVKANTPKGEIELHYDDKGPLAAYVWKTTADPFVGRQTFFKVYSGVMSSDTRVWN
                     ATKGEEERLAGVQVPSGKDMNAVSVLHAGDLGAVAKLSVTTTGDTLTTKEQNITLDMP
                     EYPTPLYRVAITPRTQADASKMSQTLSRLAEEDLTLKWEMEAATRQTLLFGMGDQHID
                     VAVKRA"
BASE COUNT         1146 a          823 c          801 g         1184 t
ORIGIN      
        1 gctgaagatt tgtttcagtt cctcgtagtc tgcatcggcc agttcttttg agatgcccaa
       61 aacacgaccc tggctataag tttgcacata aaagccgttt ttaacgactt cgatgctctt
      121 gacttcttct ttggggacgc gcaccttggg gaaattgggt tgtgtctgcg aaacgccttc
      181 atcatcaaca gtcagggagt attctttcca gagttgcacg ttttgtttgt ggcgtcgaaa
      241 acccatgaat ccatagaaaa caaccatcag gacaaccatg atgatagctt gattttgcat
      301 ttccgggcga tcgcgataca ggaaaaagac caggacaacc atcagcacca ggggaatgga
      361 atacatgagt gcaagaattt tagtttgttt ttttagtttt tcggggtcga attggtaggt
      421 tttcatcttc ttctccatta gtttatggga gatattatac tagataagta tgatattcgt
      481 gtaaaacgat aatataaata atcctggacc taaacagtgt tgatgacaag ttgagtagaa
      541 aaagccctta aaaactaaat gaaagtcaat acaaattctt gactgtgaca ggtaggagtc
      601 atcagattat ccgcttattt tcttatcgcc ataatattca actaaaatca aaaccaaagc
      661 ggtaaccgca aggattccat ccatgatgcc agcgggcaac aggaacatca ttaatatcat
      721 tgtagtcaca gaattaataa cagacaatac cagtccccac attctgtttt tcagtaatcc
      781 aaccgctccg ataattctca ggataccata aatcaaactc attactgaca tcatatatag
      841 gttatcttga aaatattgaa gtttgaattc aaagtattgt cccgcattac tattgctaaa
      901 gccaaaagtc aaggcagata tcaagaataa aaccccaccg atttccatta atccgccatg
      961 tatgatttgc aatattgcag ctgttctgta tcttttcatt ttcttgtatc tccgtaaact
     1021 ctatttttaa atactaagac gaaaagtatt gtctttattc cattagatta aatttgtaaa
     1081 tataggcttt ctcaccataa tggcgattac ctacccaata aacagtttta ttgctttggt
     1141 tatagataac gctatgaatt gtgacagaat agatgtcgtt atccaaattt ctcaaggcaa
     1201 ctttttctaa aacaatcatg gcgtcttcat catctttaac aagcccgcct cttttttcta
     1261 attcctcttt gactgttcgg tatctgtcaa gtccatgttg tttgtcacct tcttcataat
     1321 aaccagggta aacaataaag tttgttacca gttgatattt atctgtgcca gccaaactca
     1381 tagggtcatc attatagatg accattaatt ctcttaattg accgtctgtg tctgtagaat
     1441 ctgtgccagc aatgtattcc aggaccgcag atttgccatt ggcatcagat atcatatagt
     1501 ggaaggatga attggctgag tcatgcatgt cgtacttttc agcaattgca acagcttctt
     1561 caaccgtggc tgcataatca agtattactc ttaaaagaac tgttgatgtg atatctggtc
     1621 tgccattctc ttggtttgtg ggtacgttta ttttccctgg tccttgatgg gtcatataga
     1681 taccaacagt tagtcctttt tcattcattc cgtcaagggg agcgtaggct gcagctatgg
     1741 tgttgatctt atttctcaaa ccttccattt tcttgtcact gggtacacct aaaaagctca
     1801 aatcaacggt tgatatagac ttatacctgt ttgctggatt ggtatgaaca atggcaacgt
     1861 tggttttgta catatcataa tttcttgcaa atattttgtc gccatttgta gcattcgctg
     1921 agaatgatga gcagcctaca atatctgcct cttcaatgga caaattaaat agtccctttg
     1981 tgatttttga tgtcaaaaag ctgattaatt ccttgtcgtt ctttgtgcca cctgcttcca
     2041 ggtattcatc aagatagtag tctccgaaaa ctgtcatttc atatacatag ccattcgtgt
     2101 catcatcacc cggcattatt tcttttatgc ttgctacagt tctgatttca ttcttccaaa
     2161 taaaacctgc aagcagcaat aatgagaatg ccagggacag gatagataga agaatagttt
     2221 ttatcataga tttattgcgc ttatttttca tattaaaacc tcgtatcatt ttgcatgatg
     2281 catcatctca aaagttatga aatgatttta gttaagcaat gcaggggttt caaagcaatg
     2341 tatgatcttt gtacataaga tttatgtatg atattcactt caattatctt gttgttatta
     2401 ctataagtgc atttttattg atgaataaat ttccaagatg aaacacattg taaattcatt
     2461 aaaaattgtc aatatgatgg aatatctgat ataatcaaaa gtcggtttac tgccgaataa
     2521 taacgattaa agataaaaaa ctagccaggc gcagcgccag gcaaaaatga taacaggaag
     2581 gtaatacatg aaagaatttt caaccgcatc aattcgcaat attgtgctcg catcacacag
     2641 caactcgggc aagactatgc tcatcgagaa tatgttgaat tttactggcg ctaccacccg
     2701 catcggaaaa attgaagatg gcacaacgac atcagactgg gacgaagaag aacaaaaacg
     2761 acaaatctca ctcttttctt cgctggttcc tatcctttac aaggacatta aatttaactt
     2821 tattgacacc ccaggctatt ccgactttgt tggtgaagtg gtttccgcaa tgcgtgttgc
     2881 tgatgcagcc ttgattttgg ttgactctgt ttctggtgct gaagtaggta ccgaaaccgc
     2941 tttgtactat gctgaaaaat tcggaatgcc ccgctttttg cttctcaaca agatggatcg
     3001 cgataatgcc agttttgcaa aagccatgaa atctgtagaa gaagtaaccg acgcacgctt
     3061 aatcccgatg catttaccca tcggcgaaaa agctgatttc aaaggcgttg tagacttact
     3121 gacgatgaag gcctatcttg gcgacggtaa aactgccagc gatatccctg ctgaaatgca
     3181 agctgaagcg gaagaggcgc gcacagtgct tgtggaagca gctgctgaag gcgaagatgc
     3241 gcttttggag aaatatttcg aaaatggcga tttgaccccc gaagaaatgg ttcagggttt
     3301 acacaaagcc attcaggtca gcaatattat ccccgtgatg gtcgccgctg gtggacgtat
     3361 gatgggcatc ggcccgattt tggaaggatt gcttgcttta gcaccctcac ctttggaacg
     3421 accggctgtg aaagcgaaca cgcccaaagg cgaaattgaa ctgcactatg atgacaaagg
     3481 tcccctggcg gcttatgttt ggaaaacaac cgcggacccc tttgttggcc gacagacttt
     3541 ctttaaggtt tattctggtg tgatgagctc cgatacccgc gtatggaacg ccaccaaagg
     3601 tgaagaagaa cgtttagctg gtgttcaggt gccatcgggc aaagatatga acgcggtgtc
     3661 tgttttgcac gccggtgacc tgggcgcggt tgccaagttg agtgtaacca ccactggtga
     3721 cacgttgacc accaaagagc aaaatatcac tttggacatg cccgagtatc ccacacccct
     3781 ttatcgtgtg gcaatcactc cacgcacaca agcagacgcc agcaagatga gccagactct
     3841 ttcgcgcctg gcagaagaag acctgaccct gaaatgggaa atggaagctg ctacccgcca
     3901 aaccttgtta ttcggtatgg gcgaccagca catcgatgtg gcagtgaaac gcgc
//
LOCUS       DAOSFC010000121         3930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_21061_length_3930_cov_4.619355, whole genome shotgun sequence.
ACCESSION   DAOSFC010000121 DAOSFC010000000
VERSION     DAOSFC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21061_length_3930_cov_4.619355"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1026)
                     /locus_tag="PK791_04000"
     CDS             complement(169..1026)
                     /locus_tag="PK791_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92510.1"
                     /translation="MQEIAPNIFIEQNSLGMVTGIIRSENSTTLVDFPFRQDELKSWR
                     GATSRMVSGDARYLIMLDTHYDRLLSSKGNDCTLIAHTNTVVPGRMKVTNVRGQDEPI
                     IHTESYDPTGNVARLLPPEITFEDRMVLYLDEYEVILKHRPGANYAAIWVELPQQKII
                     FVGDSVMVDQPPYLGFSTPKIWVDEMAILADKDYRGYQIVSTRDGIVSQEDVSYWGKV
                     IKFISDKFDALLAAQAEIEEWYEIIPDVMNHFNPLMREDERYYNRLRWGIRTWYDQAI
                     SQKADENND"
     gene            complement(1077..1793)
                     /locus_tag="PK791_04005"
     CDS             complement(1077..1793)
                     /locus_tag="PK791_04005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH92511.1"
                     /translation="MLEKKRIWIIIAIALIVLAVLLLLAWTYLNGRNGPVWQFILDPE
                     AHQDWVTPAGYQCQDAPFSIPTDGFIGYLYGDVFQVGKRHQGIDIFAGTESGVTPVYA
                     PYDGYLTREESWKSTIIIRVPQDPLQPDRQIWIYMTHLADRYGNNLIDELFPPGAKEI
                     PVKQGQILGYQGDYSGNPDRPVGVHLHLSIVKDDGAGIYLNELVFSNTLDPSPYFGMY
                     LNAKTALKGPPRCIRDTGVD"
     gene            1940..3031
                     /locus_tag="PK791_04010"
     CDS             1940..3031
                     /locus_tag="PK791_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92512.1"
                     /translation="MKKYKAFTLFLCIALLVPALFGVGKVFAANPSFTIGEVVTDKTV
                     TILIKDAPKNREFIVRMGEYDTKAINGIEAGRFQTGEGGNLQATIDIPVAFAGRAQIA
                     IRMDSVTGGWYYYNWFWNKKEGGTWPPPSTTPEPTPSSPVTTPQPTKTPPKIVPTITI
                     ISAVESQSVTIAITNFPKNAEMRAFMGEMYTKGKDGIPVGDFTTTDAAGTEVTLNIPE
                     ALANDERVAIRVESVRGGWYSYNWFWNKQGGRVVTPPSSGTVTPAPTQPGDTSKYPAL
                     SIISVDSGKTVTIKADNLPQNTEFTVLLGKMWTRGVNGIPSATFNSGTQTSAELTVDI
                     PASLANESLISIRIQGTNGCYAYNWFKNK"
     gene            3146..3535
                     /locus_tag="PK791_04015"
     CDS             3146..3535
                     /locus_tag="PK791_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92513.1"
                     /translation="MEINNKITIIEGPTPEFEPVNQERGYGTPHPWALGVLEGPNFYN
                     TAFTALRTFNSQLLLERCETAWAADLPMYLVYRDPIGLQVETQILAARAIQVEEGELI
                     LLWVRNGIDEKKSDIPATPPRHGNEEE"
     gene            complement(3578..3650)
                     /locus_tag="PK791_04020"
     tRNA            complement(3578..3650)
                     /locus_tag="PK791_04020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3615..3617),aa:Arg,seq:cct)
     gene            complement(3675..>3930)
                     /locus_tag="PK791_04025"
     CDS             complement(3675..>3930)
                     /locus_tag="PK791_04025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH92514.1"
                     /translation="ADSGVVVYAGWLTGGYGNFVVIDHGNGFQTLYEHLNTIGVRCGV
                     SVSQGQVIGTAGTTGNSTGPHLHFEIRYNGTSVNPWDYLP"
BASE COUNT         1132 a         1001 c          804 g          993 t
ORIGIN      
        1 aaaggcaata cctgaaaggg tttcatcgaa ctgcgcggaa aagtaatcaa attcgggtct
       61 ccacctgatg caagcaaatt tccttcagga tctacgactg caaaagcgcc aaaatgaatt
      121 gactcaacaa tccccccgcg ggtcagcgaa acaacagcgt cataaccatt aatcattgtt
      181 ctcatctgct ttctggctta tagcctggtc ataccaagtg cgaatacccc aacgcaagcg
      241 gttgtaataa cgttcatcct cgcgcatcag gggattgaaa tgattcatta catcaggaat
      301 aatttcatac cattcttcaa tttcagcctg cgctgcaagc aaagcgtcaa atttatcaga
      361 aataaatttg attaccttcc cccaatagct aacatcttcc tgactaacga tgccatcgcg
      421 cgtggaaaca atttgatagc cacgataatc tttatccgcc aaaattgcca tttcatcaac
      481 ccaaatcttt ggtgttgaaa accccagata aggtggctgg tcaaccataa ccgagtcgcc
      541 cacaaagata atcttctgct ggggaagttc cacccatatc gcggcatagt tagcacctgg
      601 ccggtgtttt aaaatgactt catactcgtc aagatatagc accattctgt cttcaaaagt
      661 aatctctggt ggtaagagcc gcgccacatt accagtaggg tcataagatt cggtgtgaat
      721 aatcggctca tcctgcccac gaacgtttgt aaccttcatg cgtccaggca caaccgtatt
      781 tgtatgcgca ataagcgtac aatcattacc ctttgaactc aacaaacgat cataatgcgt
      841 atcgagcata atcaggtagc gtgcgtcgcc cgagaccatt ctgctggtcg ccccacgcca
      901 ggacttgagt tcatcttgtc ggaatgggaa gtcaaccaat gtggttgagt tttccgagcg
      961 gataatgccc gttaccatac cgagagagtt ttgttctata aaaatgttag gcgcgatttc
     1021 ctgcacagtg agcctccgaa atttcgattg tattgattat agctaaactc ccgcccttaa
     1081 tctacaccag tatcccgaat acagcgtggc gggcctttta gcgcggtttt cgcattgaga
     1141 tacattccaa aatagggcga tggatccaaa gtatttgaaa agaccaactc attgagataa
     1201 atccccgcgc catcgtcttt aacaatcgac agatgcaaat gcaccccaac tggtcggtct
     1261 ggatttccgg agtaatcacc ctgatagccc aggatttgac cctgttttac gggaatttct
     1321 ttggcaccag gtggaaaaag ctcatcaatc aaattgttac catatcggtc agccaggtgg
     1381 gtcatgtaaa tccatatttg tcggtctggc tgaagtgggt cttggggcac acgaatgatg
     1441 attgtggatt tccagctttc ttcccgcgtt aaatagccat catagggcgc gtaaactgga
     1501 gttacgccgc tttctgtacc cgcaaaaata tcaatgccct gatgtcgttt acccacttga
     1561 aaaacatcac catacagata gccaataaag ccatccgtag gaatgctaaa aggagcatcc
     1621 tgacactgat atccagctgg ggttacccag tcctgatgcg cttctgggtc taatatgaat
     1681 tgccacactg ggccatttcg tccattaaga taagtccatg caagcaaaag caaaacagct
     1741 aacacgatta gcgcaatggc gataataatc caaatccttt ttttctcaag catcataatc
     1801 cgatttttaa gtctacagtt ctgttttgtc cgattattat acgccccaaa aactttttta
     1861 caaaattgtg agcttaggaa caaatcttgc atggaatgcg ttgtatatta aaagaacttg
     1921 acgtcgaaaa ggagaaacaa tgaagaaata taaagctttc accttgtttt tatgtattgc
     1981 cctgttagtc ccagcgttgt ttggcgttgg taaagtcttt gcggcaaacc caagttttac
     2041 aataggagaa gttgttaccg ataaaaccgt gactattctc atcaaagacg cgcctaaaaa
     2101 tcgtgagttc atcgtgcgca tgggcgaata cgacaccaaa gcgattaacg gcattgaagc
     2161 tggtcgtttt cagactggcg aaggcggcaa tttgcaagcc actattgata tccctgtcgc
     2221 tttcgcaggg cgcgcccaaa tagccatccg catggatagc gtcactggtg gctggtacta
     2281 ttacaactgg ttttggaaca aaaaagaagg tggcacctgg ccacccccat caaccacacc
     2341 cgaaccaaca ccatcatcac ctgtcacgac tccccagcca actaaaacac cgccaaaaat
     2401 tgttcccacg ataacaatca tatcagcggt cgaaagccaa agtgtgacca tcgccattac
     2461 aaattttccc aaaaatgctg aaatgcgcgc ttttatgggc gagatgtata ccaaaggcaa
     2521 agatggtatt cccgttggcg attttacaac caccgacgcg gcaggaaccg aagttactct
     2581 aaatatcccc gaagccctgg caaacgatga acgcgttgcc attcgtgtgg aaagtgtgcg
     2641 tggtggctgg tattcctata actggttctg gaacaaacaa ggcggtcgtg tcgtcacccc
     2701 accaagttca ggaactgtca cgcccgcgcc aacccaacct ggcgacacaa gcaaatatcc
     2761 cgcactcagc attataagcg tcgattcagg caaaactgtc actataaaag cggataactt
     2821 gcctcaaaat acagaattta ctgtcttgct tggcaaaatg tggacccgtg gcgttaatgg
     2881 cattccttca gccactttca attctggaac acaaacgagt gcggaactaa ccgttgatat
     2941 ccccgccagt cttgctaatg aaagtttgat ttctatccgc attcaaggaa ccaatggctg
     3001 ttatgcctat aactggttca aaaacaaata aacccatacg attctcattt tcgtatccga
     3061 aagccggcta accaccggct tttcactttc ctgtttccat ttacaagtta tcaataataa
     3121 accgtcgtta tggtatcatt taaggatgga gataaacaat aaaatcacga ttatagaagg
     3181 ccccacgccc gagtttgaac ccgtcaacca ggagcgtggc tatggcacac cccacccttg
     3241 ggcgctgggt gtgcttgaag gccccaactt ttacaatacc gccttcacag ctttgcgtac
     3301 ttttaacagc cagctcttgc tggaacgttg cgaaactgcc tgggcagccg acctgcccat
     3361 gtatctcgtc tatcgcgacc ctatcggctt gcaggttgaa acacaaatcc ttgccgctcg
     3421 cgccattcag gtcgaagaag gtgaactaat tttgctatgg gtacgcaatg ggatcgacga
     3481 aaagaagtca gacatccctg ccaccccacc cagacatggg aatgaagaag aataaacaca
     3541 cttaagcaaa aacacccaaa cgggtgtttt tcttttatac cccaggaggg aatcgaaccc
     3601 ccaatccagg cgttaggagt gccttgctct atccattgag ccactggggc gatagctctc
     3661 tgagtatatc acctttatgg caaataatcc caggggttta ctgatgtgcc gttatatcga
     3721 atttcaaaat gaaggtgagg ccctgtagaa tttccagtag tgcctgccgt gccaatcacc
     3781 tgaccctggc tcacactcac gccacatcta acaccaattg tgttcaaatg ttcataaagt
     3841 gtttgaaaac cattaccatg atcaatcaca acaaaattac catatcctcc cgttaaccaa
     3901 cccgcataaa ctacaacacc actatccgcc
//
LOCUS       DAOSFC010000122         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_21153_length_3917_cov_4.224754, whole genome shotgun sequence.
ACCESSION   DAOSFC010000122 DAOSFC010000000
VERSION     DAOSFC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21153_length_3917_cov_4.224754"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PK791_04030"
     CDS             <1..371
                     /locus_tag="PK791_04030"
                     /inference="COORDINATES: protein motif:HMM:NF027523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92515.1"
                     /translation="SLANDVGQEMGSGATLINLRRTKNGRFTLRDAVSLRKLEEAFAD
                     GDWYRYLIPAADALADWYTVELEFDQVDAVRHGHRVPAVEPREPGSLARAVSQEGELV
                     AVVEYIEEDQEWQPRKVFLL"
     gene            430..1368
                     /locus_tag="PK791_04035"
     CDS             430..1368
                     /locus_tag="PK791_04035"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOH92516.1"
                     /translation="MSCDTRFTMGWHLRDDAPVWLTIGNFDGVHLGHQALLADLIRLA
                     RQDNTRSVLLSFSPNPKVFFSGEKGFYLSTPGEKTALLSRLGIDDVLIIAFERKLANM
                     AATDFMAELTRRINLKGFVVGEDFVFGHNRQGTTEVLAEFCEKQGIPFVVFPELMMDG
                     EPVSSTRIRRALNDGKVDEARRLLGRPYAMCAKVISGEQIGKSIGVPTANLELDPDKF
                     LPKRGVYATIAHLRGEDYPAVTNVGVRPTFSEQEIVSVETLILDFNDDIYGEELRVEF
                     IQCLRTEQKFDSVQAVTQQIEKDKLITRRIFENGLK"
     gene            1355..2785
                     /locus_tag="PK791_04040"
     CDS             1355..2785
                     /locus_tag="PK791_04040"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOH92517.1"
                     /translation="MDLNRRVYRLSPRDFGAETIAVAFAKTSRSPEPFDVIANELNDD
                     KSREFSEKWIVGYGHSSVAEHAVLHLALENVSRLAIETVEANRLASYTEKSTRYQEWD
                     PNAFVVPPEVIGTEYEKPYRDYMRANFDFYAKALEALRPWGEANTPRRENESNRAWAN
                     RARVKAVDVARFVLPMAAMANVGVTMNARTLEYAIKKMLSSELAEVREIGAEIKRVSN
                     EELPTLVKYADEIEHLPAMRERCRAFAEQIDAVPDDEWCHIHQFENNGEERILAAVLY
                     RFGDASYDVCFDQVIKMSLEERQAIVDALFEGMNPFDVPIREIEYANLSFDLVMDQGA
                     YFEFKRHRMMTQTPQDLSTRLGYSVPLAITEAGIEDEYRAIMKQASDVYELIAAWNPQ
                     VAAYVVPNAYNRRVLCQTNLRALSHFISLRTAGNAHFSIRRVARAMLEKASEIYPLIC
                     STIRLKNNETSEGLTKEYFTAVADKA"
     gene            2926..3645
                     /locus_tag="PK791_04045"
     CDS             2926..3645
                     /locus_tag="PK791_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOH92518.1"
                     /translation="MCGRFTMALDAERLQMKLDLASMPEPWAKRYNIAPSQYIPTVPF
                     AESRHVVYMRWGLVPFWAQDPAIGNRMINARAETVAEKPSFRSSFKNKRCLILADGFY
                     EWQKASGGKSSQPYYFTLKTREPFAFAGLWDDWEPKGVSSEPGIPGLTTCTLITTTAN
                     RVVAPVHPRMPVILTKERMWDWLTAKTETEKLALLKPLEPELMQAWPVGREVNSPAID
                     DESLIHPIDAMNQSIKQEQLL"
     gene            3642..>3917
                     /locus_tag="PK791_04050"
     CDS             3642..>3917
                     /locus_tag="PK791_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92519.1"
                     /translation="MKKTYNKSLLFLTLIVLLSSIILSACQPKPPKAWWKDVVFYEIF
                     VRSFKDSNGDGIGDFNGIIEMLDYLNDGDPNTTHDLGIGGIWLMPINP"
BASE COUNT         1012 a          868 c         1050 g          987 t
ORIGIN      
        1 gttcgctggc gaatgatgtc ggccaggaaa tgggctccgg ggcgacgctg attaaccttc
       61 gacgtacgaa aaacggacgc tttaccctgc gcgacgcggt tagcttgcgc aagctggaag
      121 aagcctttgc tgacggagac tggtaccgct atttgattcc tgctgctgat gccctggcag
      181 actggtatac cgtggaactt gagtttgacc aggttgacgc ggttcgccat gggcaccgtg
      241 ttccggcggt tgagccgcgt gaaccgggta gcctggcaag agcggtgagc caggaaggcg
      301 aattggttgc tgtggttgag tatattgaag aagaccagga atggcagcca cgcaaagtgt
      361 ttttgttgta aaaaacgctg gcagttttat atgactgtga gcgatagagt aaaagagtaa
      421 aaaagacgaa tgagttgcga caccaggttt acgatgggat ggcatctgcg cgatgatgcg
      481 ccagtttggc tcaccatcgg gaattttgat ggtgtccatc ttgggcatca agccttgctt
      541 gctgatctta tccggcttgc cagacaagat aatacccgtt cagttttgct tagttttagc
      601 cctaacccca aagtgttttt ttctggggag aagggctttt atcttagcac gcccggcgag
      661 aaaaccgcct tgctttccag gctgggtatt gatgatgtgc taatcattgc gtttgagcga
      721 aaactggcaa acatggcagc aacagacttc atggcagagt tgacgcgacg aatcaattta
      781 aagggctttg tggttggtga agattttgtt tttggccaca accggcaagg cacgactgaa
      841 gtgctggcgg aattttgcga gaagcaaggc attccttttg tcgtttttcc agagttgatg
      901 atggacggtg agccggtttc atctacccga attcgcaggg cattaaatga tgggaaagtt
      961 gatgaagcca gacgactgtt ggggcgacct tacgccatgt gcgcgaaggt gattagtggt
     1021 gagcagattg ggaagtcgat tggcgttcca accgcgaacc tggaacttga ccccgataaa
     1081 ttcctgccta aaagaggagt ttatgccacg attgcacacc tgcggggaga agattatcca
     1141 gcggttacca acgttggcgt tcgtcccaca ttcagtgagc aagaaattgt gagtgtggaa
     1201 acactcatct tggattttaa tgatgatatt tacggcgaag aattgcgcgt agaatttatt
     1261 cagtgcttaa ggactgagca aaaatttgac agtgttcaag ccgtgacgca acaaattgaa
     1321 aaagacaaac ttatcaccag gaggatattc gaaaatggac ttaaatagaa gagtttacag
     1381 acttagcccc agagattttg gcgcggagac aattgcagtc gcttttgcca aaacatctcg
     1441 ttcacccgaa ccctttgacg ttattgctaa cgagctcaac gacgataaaa gccgtgaatt
     1501 cagcgaaaaa tggattgtgg gttatgggca ctcaagcgtc gcggaacatg cggttttgca
     1561 tctcgcgctg gaaaacgtct cgcgtttggc aatcgaaacg gttgaagcca accgcctggc
     1621 atcttatacc gaaaaatcaa cccgctatca ggaatgggat ccgaacgcgt ttgtcgtgcc
     1681 accggaagtc attggcacgg aatatgaaaa accttaccgc gattatatgc gcgcgaattt
     1741 cgacttttac gcaaaagcat tggaggcttt gcgcccgtgg ggtgaggcta atacgccccg
     1801 ccgcgaaaat gagtcaaatc gtgcctgggc gaatcgtgcc cgtgtgaaag cggttgatgt
     1861 cgcgcgcttt gtgttgccta tggctgccat ggcgaatgtg ggcgttacca tgaacgcgcg
     1921 caccctggaa tatgccatca agaagatgct ttcttctgaa ctggcggaag ttcgcgagat
     1981 aggcgctgaa attaagcgcg tttcaaacga agaattgcca accctggtga aatacgctga
     2041 tgaaattgag catttgcctg ccatgcgcga acgatgtcga gcttttgccg agcaaattga
     2101 tgctgtgcct gatgacgaat ggtgtcatat ccatcaattt gaaaacaacg gtgaagaacg
     2161 catcttggcg gctgtgcttt atcgtttcgg cgatgccagt tatgatgtct gttttgacca
     2221 ggttataaaa atgagccttg aagaacgcca ggccatcgtg gatgcgttgt ttgaaggcat
     2281 gaatccgttc gatgtgccga ttcgcgaaat cgaatatgcg aatttgtcct ttgacctggt
     2341 tatggatcag ggtgcctact ttgagttcaa acgccaccgc atgatgaccc aaacgcccca
     2401 ggaccttagc acccgcttgg gttacagtgt gccactggcg attaccgaag ccggcattga
     2461 ggatgaatat cgcgccatca tgaaacaggc gagcgacgtt tatgaactaa tcgcggcgtg
     2521 gaacccacag gtcgcggctt atgttgtgcc taacgcctat aaccgccgtg tattgtgcca
     2581 aactaatctg cgcgcgttga gccatttcat cagcctgcgc accgcgggta acgcgcattt
     2641 ttccatccgc cgggtcgccc gcgcgatgct tgaaaaagcg agtgaaattt atccgctcat
     2701 ttgtagcacc attcgtttga aaaacaatga aacgagcgaa ggattaacga aggaatactt
     2761 tactgctgta gctgataaag cctgatagca gaccatcatt tgcgaacctg ccttttgggg
     2821 tttgtctgta gtcactgttg acagtcaatc agtaaacccg agcaagagca ggttcgtatt
     2881 atatagaata tcaatataga gcatcgcttt tgatgagaca atgtgatgtg cggtagattt
     2941 acgatggcgc ttgacgccga acgattgcaa atgaaactgg atttagccag tatgcccgag
     3001 ccatgggcaa aacgctacaa tattgcaccg tcgcaatata ttcccacggt gccttttgct
     3061 gagtcgcgtc atgtggttta tatgcgctgg ggactggttc cattttgggc gcaagaccca
     3121 gcgattggca accgcatgat taatgcgcgc gctgaaaccg ttgctgaaaa gccatccttt
     3181 cgctcgtcat tcaaaaataa acgctgcctg attctggcag atggctttta tgagtggcaa
     3241 aaagcgagcg ggggcaaaag ttcacaacct tattatttca cgcttaaaac ccgcgaacct
     3301 ttcgcctttg cgggtttatg ggatgactgg gaaccaaaag gcgtttcgtc tgagccgggc
     3361 ataccgggtt tgacgacatg taccttgatt acaaccacag cgaatcgcgt cgtggcacca
     3421 gttcatccgc gtatgccggt gatacttacc aaagagcgta tgtgggactg gctgactgcc
     3481 aaaactgaaa ctgaaaagct ggcgctactc aaaccgcttg agccagagct gatgcaggca
     3541 tggccggtag ggcgcgaagt aaacagtccg gcaattgacg atgaaagctt aatccatcct
     3601 attgatgcga tgaatcaatc cataaaacag gagcaactgc tatgaaaaaa acttataata
     3661 aaagcttatt attcttaacg cttattgtac tgctttcaag tatcatcttg agtgcgtgtc
     3721 agccaaaacc gcctaaggcc tggtggaaag atgtcgtgtt ttatgagata ttcgtgcgtt
     3781 cgttcaaaga cagtaacggt gacggtatcg gcgattttaa cggtattatc gaaatgctgg
     3841 attatctcaa tgatggcgac ccaaatacga cgcatgattt gggcattggg ggcatttggc
     3901 ttatgccgat taatcca
//
LOCUS       DAOSFC010000123         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_21251_length_3905_cov_4.017922, whole genome shotgun sequence.
ACCESSION   DAOSFC010000123 DAOSFC010000000
VERSION     DAOSFC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21251_length_3905_cov_4.017922"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PK791_04055"
     CDS             <1..422
                     /locus_tag="PK791_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92520.1"
                     /translation="PAGGSAMVGVKEVARAAVGALERGERGKLYHIASENLGWTDFLT
                     RLGHILQKPKKIITLPNWLVKFGAGLVVLYHKLQGREAGLDLLPYVQVQTRNDFLNLD
                     DAPKELGYEHCDLQEAFEATVEAYRALKEKTTKKNKM"
     gene            434..853
                     /locus_tag="PK791_04060"
     CDS             434..853
                     /locus_tag="PK791_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92521.1"
                     /translation="MKKLIDWLNPRGTKTWLLFLGIVLGGVWGVAFLALVDYLIQRYP
                     KQITGVDTTLMLGIFVGIATLSFFLAILSKDKRGMTYGLYAGLACMVACGLILYRNSL
                     TLVPLVGIMGPLGAFNGAGLGEMFNRSREARKKERDL"
     gene            complement(896..2473)
                     /locus_tag="PK791_04065"
     CDS             complement(896..2473)
                     /locus_tag="PK791_04065"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOH92522.1"
                     /translation="MAKYIHTILRDIPVQPVSDIPRVLLRGVSADSRQIKDGYAFVAV
                     KGDSVDGNQFIPNAIENGARLIVSAHPKPKGLNIAWLQIESDPRAALAHIAAALNGFP
                     ARKLRVIGVTGTDGKTTTSSMIHHLLIQSGIKAGMISTVSALIGDKALDTGFHVTTPD
                     SPDIQNYMRLMVEAGMTHCVLETTSHGLSQRRVIAADIDIAVITNITHEHLDYHGSLE
                     AYVQAKGLLVEALAQTPRKRIGNPRLAVLNKDDEHYAYTKRLAPGKYVSYSRINEADI
                     WASDIDNRPEQLRFTAHIGKQSYPVTSPMIGIFNVSNSLAALAATVLGLGLDPARAAQ
                     HIASLPPVPGRMEVINMGQDFTAIVDFAHTPNALERSLETIRLMTPGKLITVYGSAGL
                     RDREKRRMMPKVSIKGADISILTAEDPRTESLDAILEDMAQAAIEAGGVEGVNFYRVP
                     DRGDAIRKAIELAHPGDTIVAQGKGHEQSMCFIETEYLWDDRTAMRAALAEHLGVDGP
                     EMPWLPTSKANKEKELH"
     gene            complement(2475..3206)
                     /locus_tag="PK791_04070"
     CDS             complement(2475..3206)
                     /locus_tag="PK791_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92523.1"
                     /translation="MKTDKHAYLPEYFENPPYRDIIFRQIYSVLVNYMKKPYYLILIV
                     LGISLLVFGVIVYRNTDSTEEFRIERAELFDRLSNSKGYAYATLKYPSELLIKEEAVI
                     SLEIMPDKNLKNILEHGVAIDTLISPKRLVLSSERRQLEAVSASGTTISWRIEATRLG
                     TSSATVSLGLSDLRTDITIYPITPQHTFQINIPVVETKSKASFGEYIGPIMIAVGSLS
                     LLAGFVIASKDFDKATKQSRKKNKR"
     gene            3291..>3905
                     /locus_tag="PK791_04075"
     CDS             3291..>3905
                     /locus_tag="PK791_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOH92524.1"
                     /translation="MAKESKPRPKKSREREPLSPEQRKQKRKHFLEDLGQFLLCILGG
                     LIYAASINLIVLPSGLFIGNMTGLAQVVQDILKSFFPINRDITGLLLFLFNLPWFIIS
                     FTSINRKFFWKTVITVASMFIAMTFIPIVQILPETLETITITIIGAVIAGFGAGLCLQ
                     AGGSGGGTDIFGVYISMKRKEFSVGRINLIISLIIYTYAFFVKDF"
BASE COUNT          915 a          860 c         1069 g         1061 t
ORIGIN      
        1 atcccgctgg gggtagcgcg atggtgggcg tcaaggaagt cgcccgggcg gcggttgggg
       61 cattggagcg gggcgagcgt ggcaaactct atcacatcgc cagtgaaaac cttggctgga
      121 ctgacttttt aacgcgtctt gggcatatcc tccaaaagcc caagaaaatc attaccttgc
      181 ccaactggct ggtaaaattt ggggccggac tggtcgtttt gtatcacaaa ctgcaagggc
      241 gtgaagcagg actggatttg ctgccttatg tgcaggtgca aacgcggaac gattttttga
      301 acctggacga cgcgccaaaa gaacttggtt atgaacattg tgatttacag gaggcctttg
      361 aagctacagt tgaagcgtac agggcgctta aagaaaaaac cacgaagaaa aataagatgt
      421 agatggacag ataatgaaaa agttaatcga ttggctgaat ccacggggaa caaaaacatg
      481 gttgcttttc cttggcatcg ttttgggtgg agtatggggg gtcgcctttt tggcgttggt
      541 tgactatctc atacaacgct atcccaaaca gataactggg gtggatacaa ccctgatgtt
      601 gggcattttt gttggcatag caacgctttc cttcttttta gcgattctgt ccaaagataa
      661 gcgagggatg acctatggac tttacgccgg gctggcttgc atggtggctt gtggcttgat
      721 tttataccgc aactcgctga ctttggtgcc gcttgtggga atcatgggcc ccttgggcgc
      781 ttttaatggc gcgggattgg gcgagatgtt caatcgcagc cgcgaggcaa gaaaaaaaga
      841 acgcgatcta tagcgttctt ttcttagttt agttttgtgt actgaaaaag ggcttttaat
      901 gaagctcttt ttctttattt gctttactgg ttggcagcca gggcatttca ggcccatcca
      961 cgcctaaatg ctcagccagg gcagcgcgca tcgcggtgcg atcgtcccat aaatactccg
     1021 tttcaataaa gcacatcgac tgttcgtggc ctttgccttg cgcgacgatt gtatcgccgg
     1081 gatgggcgag ttcaatcgcc ttgcgaatgg catcgccacg gtctggcaca cggtagaagt
     1141 tgacgccttc cacgccaccg gcttcgatcg cggcttgcgc catgtcttcg agaatagcgt
     1201 ccagcgactc ggtgcgcggg tcttccgcgg tcaaaataga aatatccgcg cctttgatgg
     1261 acactttggg catcatgcgt cgtttttcgc ggtcgcgcaa gccggctgaa ccgtaaaccg
     1321 taattagctt accgggggtc atcaggcgaa tggtctccaa agagcgttcc agcgcgttgg
     1381 gggtgtgcgc gaagtcaacg atggcggtaa aatcctgccc catattaatg acttccatac
     1441 gtccgggaac agggggcaag ctcgcgatgt gctgcgccgc tcgcgcgggg tccagtccaa
     1501 gcccaagcac ggtcgccgcc agggcagcca ggctattgga gacattaaaa atgccgatca
     1561 tggggcttgt taccgggtag gactgcttgc cgatatgcgc ggtaaagcgg agttgttccg
     1621 gtcggttgtc gatatcgctg gcccaaatgt ccgcttcatt tatacggctg taagaaacgt
     1681 atttaccagg tgccaggcgt ttggtgtaag cataatgttc atcatctttg ttcaagactg
     1741 ccaggcgggg gttgccgatg cgcttgcgcg gggtctgcgc cagggcttca accaggagac
     1801 ctttggcttg cacgtaagct tcaagtgagc cgtgatagtc aagatgttcg tgggtgatgt
     1861 tggtgatgac ggcaatgtcg atatcggcgg cgatgacacg cctctggctt agtccgtgcg
     1921 aagtcgtttc gagcacgcaa tgcgtcatgc ccgcttcaac catcaggcgc atatagtttt
     1981 ggatgtcggg tgagtcgggg gttgtgacat gaaaaccggt atccagggct ttgtcgccga
     2041 tcagggcgct gacggttgaa atcatgccgg ctttaatgcc cgactgaatg agcaggtggt
     2101 gaatcatcga cgacgtggtg gttttgccat cggtgccggt tacgccgata acgcgcagct
     2161 ttcgggcggg aaaaccgtta agcgccgcgg cgatgtgcgc cagggcggca cgcgggtcgc
     2221 tttcgatttg taaccaggcg atgtttagac ctttcggttt ggggtgagcg ctgacgatga
     2281 gacgcgcgcc gttttcaatt gcgttgggga taaattgatt gccgtcaacg ctgtcgcctt
     2341 tgacagcgac aaaggcgtaa ccgtctttta tctggcgcga gtccgcgcta acgccacgca
     2401 aaagtacgcg tggtatgtct gaaacaggtt gaacggggat atcacgtagg atagtgtgta
     2461 tgtattttgc catgttatct tttattcttt ttccttgatt gttttgtcgc tttatcaaag
     2521 tcttttgatg caataacaaa accagccaaa agcgaaaggc taccgaccgc gatcatgatg
     2581 gggccgatgt actcgccaaa tgatgctttg gactttgttt caacgacggg aatgtttatc
     2641 tgaaaagtat gttgtggagt gatcgggtaa atggtgatat cggtgcgtaa atcacttagt
     2701 ccgaggctca ctgtggcgct gctggttccc aggcgtgtgg cttcaatacg ccagctaatg
     2761 gttgtgccac tggcgctgac agcttcgagt tgacgacgtt cagagcttag tacaaggcgt
     2821 ttgggagaaa tgagcgtgtc aatagcgacg ccatgttcca ggatgttttt cagattctta
     2881 tcgggcataa tttccaggct aataacagct tcttctttta taagaagttc tgatggatat
     2941 ttcagcgttg cgtaagcata acctttacta tttgaaaggc ggtcaaaaag ttctgcccgt
     3001 tcaatgcgga attcttctgt ggagtccgta ttacggtaaa caataacgcc aaaaacaagc
     3061 aaactaatgc caagaacgat aaggatgaga tagtaaggct ttttcatata attcaccaaa
     3121 actgaataga tttgcctaaa gatgatatca cggtaagggg gattttcaaa atattctggc
     3181 agataagcat gtttgtcagt tttcacatga tgcacaatgc ctgttttgaa aagaaaaaat
     3241 gctcagctga atcgcttgcc caccaggcat ctttgggata acattaggcc atggcaaaag
     3301 agtcaaaacc ccgtcctaaa aaatcgcgag aacgcgaacc attaagcccc gaacagcgta
     3361 aacaaaagcg caaacacttc ctggaagacc ttggacaatt tttattgtgc atccttggtg
     3421 gtttgattta tgctgcttca attaatttga ttgtgttgcc aagtgggctg tttatcggta
     3481 atatgaccgg tttagcacag gttgtgcagg acattctaaa gtcctttttc ccgatcaatc
     3541 gcgatataac gggtttgttg cttttccttt tcaacttgcc ctggtttatc atttccttta
     3601 cgtcaatcaa tcgcaaattt ttctggaaaa cggtcattac ggttgcttca atgttcattg
     3661 cgatgacctt tatccccatc gtgcagattt tgcccgagac tcttgaaacg attacgatca
     3721 caattatcgg cgctgtgatt gccggttttg gtgcgggttt gtgtttgcaa gccggtggca
     3781 gcggtggtgg tacagatatc tttggcgtgt atattagcat gaagcgaaaa gaatttagtg
     3841 tgggacgtat caatttgatt atcagcctga ttatttacac ctacgctttc tttgtgaaag
     3901 acttt
//
LOCUS       DAOSFC010000124         3894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_21330_length_3894_cov_5.160979, whole genome shotgun sequence.
ACCESSION   DAOSFC010000124 DAOSFC010000000
VERSION     DAOSFC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21330_length_3894_cov_5.160979"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PK791_04080"
     CDS             <1..432
                     /locus_tag="PK791_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH92525.1"
                     /translation="HVRYGKKVWSPVVSKKKGDIIIGFEELEVLRNLEYLRDDGEGVV
                     ILNRARVNPIGVLSGKDVYPPNIQELIEARAGRVIALDATEEAEKLGNPRTMNIILLG
                     VALKALGLHDEIDWNKIIAENVREQFVEVNQKALKLGMSLV"
     gene            complement(506..1642)
                     /gene="dnaN"
                     /locus_tag="PK791_04085"
     CDS             complement(506..1642)
                     /gene="dnaN"
                     /locus_tag="PK791_04085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOH92526.1"
                     /translation="MKVVIQQSQLAHGVSVVARAVTPRSTLPVLSNILIKTDGERLRL
                     SGTNLELGISAWIDAKVEKEGGLTVPARTFADLVSNLPSREVTLTVDIPTQTLNVKCG
                     TLNTDIKGISADEFPPMPAPDLDEAIPLNVANFKEMINQVVFAASTEDSRPNLTGVHM
                     SFEDEMLEMAATDGYRISISRAPMAGVGKQKLEALVPARALSELSRVAVNGDETLKMT
                     FPPGRGQVIFHLKDVELVSQLIEGNFPNYNAIIPPTFKTRTVLSTSDMLKACKQTEII
                     AREGNYIARLDIQPDENGDGIAQLDISANSEQTGSSEVIVDASVEGVPLLISFNIRYL
                     REALEVIKTPTVILETNAPNTPGLLRPVDDESFKHIIMPMNIPR"
     gene            1857..2123
                     /locus_tag="PK791_04090"
     CDS             1857..2123
                     /locus_tag="PK791_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92527.1"
                     /translation="MFELNPWIQMILVGAIVGFITDTAMKGYKFKFLGAAVAGIVGAY
                     LANVLIKEWGISLSFIPAPWNAILISALGAGGLVAILRSIRQSS"
     gene            2278..3246
                     /locus_tag="PK791_04095"
     CDS             2278..3246
                     /locus_tag="PK791_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH92528.1"
                     /translation="MTENDKNEYDVLIIGGGPAGATAAIYTARAGLKTGVLDKGLTTG
                     ALGVTSKIANFPGVVGDVSGAELLSVMRKQATDFGAEFINDKAVGTDLGSEIKTVYGN
                     FDAYFAKAIIIATGSMGRANLIDGEAAFLGRGVSYCGVCDAAFYKGKEVAVIGKTDEA
                     LEEALYLSRFASKVHLLVPATQIEASDELCETMRHNNKIVPYYNARVREIYGKDKVEG
                     LRWANAGKPETLNQLAVEGVFVYLQGGKPVTDFLAGQLDPGEQGCLQVDRNMQTIVPG
                     VFAVGDVLCTRVRQAVIAVGDGAIAAIEAEKWLRGKKSARLDWHSE"
     gene            complement(3335..>3894)
                     /locus_tag="PK791_04100"
     CDS             complement(3335..>3894)
                     /locus_tag="PK791_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92529.1"
                     /translation="MGVTFHESCSRFESEIWPDNQQTLLRGIKLARRPYMDSYGPEVT
                     FNSIPANPVNPAQPMIVNVHIDDTLHSSYEGGIPSQNITAYRYSFDKPSWIEGANPVT
                     VNRNIGKPKSDVMFSVDVSHLSPGKHTIFVEGKDADGNWGIPTAAWFTVGSAPMPTPT
                     PVPTPGPDPMSNWIYLPIIVNPDTP"
BASE COUNT          950 a          840 c         1079 g         1025 t
ORIGIN      
        1 cacgtgcgct acggcaaaaa agtctggtcg ccggttgttt caaagaaaaa aggtgatatc
       61 atcatcggct ttgaagaact cgaagttctg cgcaatttgg aatatttgcg tgatgatggc
      121 gaaggcgtgg ttattttgaa ccgcgcgcgc gtcaacccga ttggcgtgct ttccggcaag
      181 gacgtctatc cgcctaatat tcaggagctg attgaagcgc gtgccgggcg cgtcatcgcg
      241 cttgacgcga ccgaagaagc tgaaaaactc ggcaatcccc gcacgatgaa catcattctt
      301 ttgggcgtcg cgcttaaagc cctgggcttg cacgatgaaa tcgactggaa caagattatc
      361 gcggagaacg tgcgcgagca gtttgttgaa gtcaaccaaa aggcgctcaa actgggtatg
      421 tccctggttt agtctcgccg tttgtatata aaagaaaaaa ggctgctttg aacgagcagc
      481 ctttttgatt ctaataagga aaaaattagc gggggatgtt catgggcatg atgatgtgct
      541 tgaagctttc atcatcgacc gggcgcaaca aaccaggcgt gttgggagcg tttgtttcca
      601 gaatcacggt cggagttttg ataacttcaa gcgcttcgcg caggtagcga atgttgaagc
      661 taatcagcaa gggcacacct tcaaccgaag cgtctacaat cacttcagat gaaccggttt
      721 gttcggaatt ggcagaaata tccagctggg caatgccatc gccattttca tcgggctgaa
      781 tgtcaagccg cgcgatgtag ttgccttcgc gcgcgataat ttcggtctgc ttacaggctt
      841 tgagcatatc gctggttgaa agcacggtgc gggttttgaa agtgggcggg ataatggcgt
      901 tgtaattggg gaagttgcct tcaatcagct gcgagaccag ttccacgtct ttaagatgga
      961 aaattacctg accacgaccg ggaggaaaag tcatcttcag ggtttcatca ccattgactg
     1021 ccacacgcga gagttcagaa agcgcgcggg cgggcacaag cgcttcgagc ttttgcttgc
     1081 ccacacctgc catcggcgcg cgcgaaattg aaatgcgata gccatcggtt gctgccattt
     1141 cgagcatttc gtcttcaaaa ctcatgtgaa cgccggtgag attggggcgt gaatcttcag
     1201 tagatgccgc aaacacaacc tggttaatca tctctttgaa gtttgccacg ttcaatggga
     1261 tggcttcatc aaggtcgggt gctggcatag gtgggaattc atccgcgctg atgcctttga
     1321 tgtcggtgtt gagcgtgcca catttgacat ttaaggtttg ggtggggata tccacggtga
     1381 gtgtgacttc gcggctgggc aggttgctga ccaaatccgc aaaggtgcgg gctggaacgg
     1441 ttaatccgcc ttccttttcc actttcgcat caatccaggc gctgattccc agttcgagat
     1501 tggtgccaga aaggcgcaag cgttccccat cagttttaat gagaatgttc gaaagcacag
     1561 gcagggttga gcggggggtt acggcacgtg caaccacact caccccatgt gcgagttgac
     1621 tctgttgaat gacaactttc ataaaactcc tccagcgtga tgactttttt atccttttaa
     1681 gtataccatt ctttatataa aatccggata tttcgtgaaa ccgggctata atttgatata
     1741 acaattctga tatggtgaaa ttccttaccc gtgctcacga aaccgcgagt gacagcacaa
     1801 agcgcacggg tgttggaaaa tgcagaattt gatatagtgt tatttatgga ggcgagatgt
     1861 ttgaactaaa cccctggatt cagatgatat tggtaggcgc gattgttggc tttataactg
     1921 acaccgcgat gaaaggctat aaatttaagt ttctcggcgc ggcagtcgcg ggtattgtag
     1981 gcgcatatct ggcgaacgtt ttgattaagg aatggggcat ttcgctgtcc tttatccccg
     2041 cgccatggaa tgcgatttta atcagcgcgc ttggggctgg tggtttggtg gcgattttgc
     2101 gttctattcg acagtcttcc taaaactggc tgagatttgc gacctaaatc tcaagtattc
     2161 atcattaata agtggggata agcacaagat aatatcttag aaaactgtta accttgcact
     2221 atattttggc atccgctggg ggattggata gactttttaa acatttgcga ggtgctaatg
     2281 acagaaaacg ataaaaacga atatgacgtg ttaattattg gcggtggtcc tgctggcgcg
     2341 acagcggcga tttataccgc ccgggctgga ctgaagacag gtgtcctgga taaaggtctt
     2401 actaccggtg ccctgggcgt gaccagtaaa attgccaact tccctggcgt ggtcggtgat
     2461 gtatccggcg cggaactttt gagtgtgatg cgcaaacagg caactgattt tggcgcggaa
     2521 tttatcaatg acaaagccgt tggtacggac ttgggttcag agataaaaac ggtttacgga
     2581 aacttcgacg cttattttgc caaagccatc atcattgcca cgggttcaat gggcagggcg
     2641 aacctgatcg acggcgaagc ggctttttta ggcaggggcg tgtcttactg tggtgtctgt
     2701 gacgcggcat tttacaaagg caaagaggtc gcggtaatcg gtaaaaccga tgaagccctg
     2761 gaagaagccc tttatctctc gcgtttcgca tccaaagtgc atcttttggt tcctgccacg
     2821 cagattgagg catcggatga actctgcgaa acgatgcgcc ataacaataa aattgttcct
     2881 tactataacg cgcgggtgcg cgagatttac ggcaaggaca aggtagaagg cttacgctgg
     2941 gcaaacgcgg gcaagcctga aacgttaaac cagctggccg tggaaggcgt ttttgtctat
     3001 ttgcagggtg gcaaacctgt cacggacttt ttagctggac aattggatcc gggtgagcag
     3061 ggttgtttgc aggttgaccg taatatgcaa actattgttc ccggtgtctt cgcggtgggg
     3121 gatgtgctgt gtacccgtgt gcgtcaggcg gtgattgccg tgggcgatgg tgccatcgct
     3181 gcgattgaag ctgaaaaatg gctgcgtggt aaaaagtctg cccgccttga ctggcattca
     3241 gaatagaaaa gtaaatcaga attttgttcg gtctgattaa atcaaaagag ttggccaaag
     3301 cttagccaac tctttttttt agataaatcc cagtttaggg cgtgtcagga ttgacaatga
     3361 ttggcaggta aatccagttg ctcatgggat ctggcccggg tgttgggaca ggggtaggtg
     3421 ttggcattgg agccgagcca acggtaaacc atgccgctgt aggaatgccc cagttgccat
     3481 ccgcatcctt gccttctaca aaaatcgtgt gtttgccagg gcttaagtga gatacgtcaa
     3541 ccgaaaacat cacatcgctt ttaggtttac ctatattacg attgactgtg accgggttcg
     3601 cgccttcaat ccagctgggt ttgtcaaagc tgtagcgata ggcagtgatg ttttgggaag
     3661 gaatgccacc ctcataactg ctgtgaaggg tatcatcgat gtggacattg acaatcatcg
     3721 gttgcgcagg atttacaggg ttggcgggga tgctgttgaa agtaacctca ggaccataag
     3781 agtccatgta gggtcggcgc gcaagtttga tgcctcttag gagcgtctgt tgattgtctg
     3841 gccagatttc actttcaaat cgtgagcagc tttcatggaa ggtaacaccc attt
//
LOCUS       DAOSFC010000125         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_21607_length_3863_cov_4.408088, whole genome shotgun sequence.
ACCESSION   DAOSFC010000125 DAOSFC010000000
VERSION     DAOSFC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21607_length_3863_cov_4.408088"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1770)
                     /locus_tag="PK791_04105"
     CDS             complement(130..1770)
                     /locus_tag="PK791_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOH92530.1"
                     /translation="MRDFKNRAFTYSLLIALIAFFVTLTPQLAQAHLPQQDVGPGIAP
                     TPDRSDALAKGWFFYRDECGNCKHVLETIIYPMLEEYPWQIDWQFYNIDDNFSAWKQI
                     EKHFTFDHSQGLPVSILGDTILVGVNQHETQLKTLIQGAITGTHLDFPVIPGLDPTEL
                     ISSDPRQSESDNPELCSEENIGACAFDPPIYMAYFFTAGCDNCGFAEIEIAQLSKKFN
                     LEIEKFNHAETAALALWMAEHANIDPNFGAPAVFIGDHAWIGNAEIKLETMEPALEAM
                     RHEGSPRFWNDYNPSEGQSKLIEKFKGLGKLGLLLAGLIDGLNPCAFATIIFFVSYLT
                     ISNRKGREILITGGMFTLGVFLAYFVVGIGFYKVLEKLTAVVKPIGLIINIITAVLCL
                     AFAYLSIKDFFKARAGDTADMALNLPEGIRKRINKTIREGSRVTRYYWGAFVTGLLIS
                     LLEFACTGQMYLPMITSMISMDSMRGQGILWLGLYNLMFIIPLVVVFVLAYFGTTSKQ
                     LTAFFKKHAAAVKLGLALIYIALAIFLIVDILQRFGIA"
     gene            complement(1908..3764)
                     /locus_tag="PK791_04110"
     CDS             complement(1908..3764)
                     /locus_tag="PK791_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH92531.1"
                     /translation="MVTIANVSELRPRMDNELDACNFVPRTPNIQKAWDWGKNAHAGQ
                     LRLSGEPYFETHCAWVASFIDKLVGIESWTIAALLHDAVEDTGESFEEIKALFPGELG
                     EKVSHIIDGLTKMSSPRDGSSREIATLRKIAMFRDPAVFLVKLADKSHNLMTLQYQSP
                     SKQWQKATEAIRAYGKLAGILNCYSWRRWIEDMSFPFAEPDSYQRVKDRIDADPRLNT
                     NFIRYYLSELANLMTAEGLEGEVRFTVNGYWQSWDKLQRMARARKTSLQDFSALNDLI
                     SFRMILKEPSEAACYQLLGRVNKYFSKLLDQDRFDDYIAAPQNGYRALQVTSYLPGSG
                     AIEVAIATEEMEGENTWGVVYCINNNKDISKYSPVQILTPFGGTRFLQEPATVLDGVA
                     AIQEFYLDKIKKVVVNGEERHIYDRLNPGDVVEVITGATAKVPEPEWLNHCSASTARR
                     LRVVLARVTLKEASKKGKAMSHAVLAERGILDLEDITALEPGRITTLLGMLACANVDD
                     LYAAIGEGSILLREFEDTLDLVGIGSTSPRWTSLLVTGSGKINRPGILADIAKLISES
                     QVNILRTVNHTFSDGTFQLRMVMPGLSQEQIAGLYQLLDENKTFPLEKVEIA"
BASE COUNT          944 a          905 c         1028 g          986 t
ORIGIN      
        1 ttcgggagcc tgggtgcggg ttctgtcaac gtacgcgaaa tttgccaaga aactgtttta
       61 atcccgaaca ctcattaaaa caaaagcgcc atcattttga tggcgttttg agttaatcat
      121 ttcagtcaat tatgctatgc caaaacgttg caagatgtca acaatcagga aaatagccag
      181 cgcgatgtaa atgagtgcca agccaagttt gaccgcggcg gcgtgctttt tgaaaaaggc
      241 cgtgagttgt ttggaggttg tgccaaagta agccagcacg aacacgacca ccaaggggat
      301 gatgaacatc aggttatata gtccaagcca caaaataccc tgaccacgca tactatccat
      361 gctaatcatt gaggtaatca tgggcaggta catttgaccg gtgcaggcaa attcgagcag
      421 ggaaattaac aaaccggtta cgaaagcccc ccagtaatat cttgttaccc ttgaaccttc
      481 gcggatggtt ttgttgatgc gcttgcggat gccttcgggc aggttgagcg ccatgtcagc
      541 ggtatcgccg gcgcgggctt tgaaaaagtc cttaatgcta agatacgcaa acgcgagaca
      601 caaaacagcg gtgatgatgt tgataatcag tccgatgggc ttgacgacag cggtgagttt
      661 ttcgagcact ttgtagaaac cgataccaac gacaaaataa gccagaaata cacccagggt
      721 aaacatgcca ccggtgatta atatctccct gcctttacgg ttactgatgg tgagatatga
      781 aacaaagaaa ataatcgtgg cgaacgcgca gggatttaaa ccatcaatga gcccggccaa
      841 aagcagtccc agtttgccaa ggcccttgaa tttttcaatc agtttacttt gcccttcaga
      901 agggttataa tcgttccaga accgtggcga accttcgtgg cgcatggctt caagagcggg
      961 ttccatcgtt tccagtttga tttcagcgtt gcctatccag gcatggtcgc cgatgaaaac
     1021 agccggcgcg ccaaagttgg ggtcaatgtt ggcatgttct gccatccaca gtgccagcgc
     1081 ggcagtttcg gcgtgattaa atttttctat ttcgagattg aactttttac tgagttgagc
     1141 aatttcaatt tcagcaaaac cacagttatc gcagccagcg gtgaaaaagt aggccatgta
     1201 aatgggtgga tcaaaagcgc aagcgccgat attctcctcg gagcataatt ccggattgtc
     1261 tgattcgctt tggcgtgggt cagagctaat taattctgtt gggtctaaac ctggaatgac
     1321 cggaaaatca agatgagtgc cagtaatcgc gccctgaatt aaagttttta gttgggtttc
     1381 gtgttgatta acgcctacca aaatggtgtc gcccaaaatg ctgacgggca agccctggct
     1441 gtgatcaaaa gtgaaatgtt tttcgatttg tttccaggcc gaaaagttgt catcaatatt
     1501 gtaaaattgc cagtcaatct gccagggata ttcttccagc atggggtaga tgatggtttc
     1561 aagcacatgc ttgcagttgc cacattcatc gcggtagaag aaccagcctt tggctagagc
     1621 atcgctgcgg tcgggtgtgg gcgcgatgcc cggtccgacg tcttgctggg gcaggtgagc
     1681 ctgggcaagc tggggcgtga gcgtcacaaa gaaagcgatt aaagcaatca acaaggaata
     1741 agtaaaagca cggtttttaa aatctcgcat aagaaaatcc tatggtgtgt ttatgtgata
     1801 tcttccaaac gattattcta acacgctttt ataaacggca aataaaaacc agctaattgc
     1861 gctggggaca tatgattaat aatttgctgt atcagtaggg tgaaagttta ggcgatttcc
     1921 actttctcca gtgggaaggt tttgttttcg tcgagcagtt ggtaaagtcc ggcgatttgt
     1981 tcttgtgaca aaccgggcat gaccatgcgt agctggaagg tgccatcact aaaggtgtga
     2041 ttaacggtgc gcaaaatgtt cacctgagat tcagatatca gtttggcgat gtcagccagg
     2101 attcccggtc ggttaatctt ccccgagccg gtaacaagca aagatgtcca gcgggggctg
     2161 gtgctgccga tgcctaccaa atcgagcgtg tcttcgaatt cgcgcaggag aattgagcct
     2221 tcgccgatgg cagcgtaaag gtcatctaca ttcgcgcaag ccagcatgcc caaaagggtc
     2281 gtgattcgac cgggttccag ggcggtgata tcttccaggt ccaggatgcc cctttcagcg
     2341 agcacggcat gactcatcgc cttgcctttt ttgctggctt ctttcagggt tacgcgcgcc
     2401 aaaaccacgc gcagtctgcg ggcggtgctg gcgctgcaat ggttcagcca ttccggttcg
     2461 ggcactttgg cagtcgcgcc ggtaatcact tccaccacat cgccggggtt gagccggtca
     2521 taaatgtggc gttcttcgcc gttcacaacc acttttttga ttttatccag ataaaactcc
     2581 tgaatggcgg caacaccatc cagcacggtg gcaggttcct gcaggaagcg cgtacccccg
     2641 aaaggcgtca ggatttgcac cgggctatat ttgctgatgt ctttgttgtt gttgatgcaa
     2701 taaaccacgc cccaggtgtt ttcgccttcc atttcttcgg tggcgatggc gacttcaatg
     2761 gcgccggagc cgggcagata gctggttacc tgcagggcgc gatagccgtt ctgcggggcg
     2821 gcgatgtagt catcaaagcg gtcctggtcg agcagtttgc taaagtattt gttcacccgt
     2881 cccaaaagct gataacaggc ggcttcgctg ggttctttta gaatcatgcg gaagctaatc
     2941 aggtcgttta gcgcggaaaa atcctgcaag gatgttttgc gggcgcgcgc catgcgctgg
     3001 agtttatccc agctttgcca gtaaccgttc accgtaaagc gcacttcacc ttcaagccct
     3061 tcggcggtca tcaggttggc aagttctgaa aggtagtaac ggataaagtt ggtgttcagg
     3121 cgcgggtcgg cgtcaattct gtcttttacg cgctggtagg agtctggttc cgcgaaagga
     3181 aacgacatgt cttcaatcca gcgtcgccag ctgtagcagt tcaaaatgcc cgccagtttg
     3241 ccataagcgc ggatggcttc ggtcgccttc tgccattgct tgctgggtga ctggtactgc
     3301 agggtcatca ggttgtggct tttatcggca agttttacca gaaagacggc agggtcgcgg
     3361 aacatggcga ttttgcgcaa ggtggcaatt tcgcgcgaag acccatcgcg cgggctgctc
     3421 attttggtca gtccgtcgat gatgtggctg actttttcgc caagttcgcc ggggaagaga
     3481 gccttgattt cttcaaaact ttcgccggtg tcttcaacag cgtcgtgaag cagcgcggcg
     3541 atggtccagc tttcgatgcc caccagctta tcaataaacg aagcgaccca cgcgcagtgg
     3601 gtctcaaaat aaggttcgcc agacaggcgc agctggccag cgtgcgcgtt tttgccccag
     3661 tcccaggcct tttggatgtt gggtgtgcgg ggcacaaaat tgcacgcgtc cagttcatta
     3721 tccatccgcg gacggagttc gctgacattt gcaatcgtga ccatatctgc accttaaagt
     3781 ttgaaaggaa gtatagctta aaaagcgtaa aattcctaat attgcttgaa tttaagccat
     3841 actaattttg ttcaagcata tta
//
LOCUS       DAOSFC010000126         3843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_21790_length_3843_cov_4.587909, whole genome shotgun sequence.
ACCESSION   DAOSFC010000126 DAOSFC010000000
VERSION     DAOSFC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21790_length_3843_cov_4.587909"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /locus_tag="PK791_04115"
     CDS             complement(<1..2158)
                     /locus_tag="PK791_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOH92532.1"
                     /translation="MNAKNHPGPKQTLDDILNFKSEADTLRVSNDSRQKKLEIIAKVS
                     KSVSETLDLNRLLKRITSLVHKEFGYPFVHIYLVHYVPQTVEFAFGSGSRAKFYNEVK
                     VSFDIHSPIGLIPLAVRTGKTQLSNKVSTDPQYLLYRPEESLTSSELVVPLIYGNQIL
                     GVLDVQSDKENAFGPEDVEVLETLSAYIAIAINNANMYRSEVWRRQGAESLRDIALLL
                     ATNVGLKELFQTVFKKMMALLPSKIASIWIFDTLEEDQKPTQFRLVSSRTQPNTIKKP
                     KNYTVNIDDVWFSKAIHEDQVIIRLSGDKTDPFYQHGYLDEEFSAIAAPLSTGGKQLG
                     VLILHENSFGRYGNEARNIAASFSGYTAVALENARLSAQSIDQAWVATILLQVALATQ
                     TLTTIPELIETIGQLTIALVGGRMGALLLPCTDTQYLSVAAVYGKTSPKKDDDPINII
                     FHDDLLWDTCQKPVERAIPAPAFSSKFKKAFKLRDNETVYLFPLVVHNQTLGILMHIS
                     KSDAEQSEPIHLLGKQRFTILQGIAQQTAINLQNINLVNERQEESYISNVLLQTSNYL
                     VTSGSLLCALDRLCQSLTCFVGAEDILILQRKDDGTHIVRTHIISSQANIDDLSNELP
                     ALSFDTLSENDVFNSPVIKPYSWYQQLLSRNPSTKSSKTDEIHTVIAPLTVPGENYGL
                     LIINVRQLKEKDKRLKLIHEIANQIASAIQNNQVQDF"
     gene            complement(2177..2602)
                     /locus_tag="PK791_04120"
     CDS             complement(2177..2602)
                     /locus_tag="PK791_04120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH92533.1"
                     /translation="MKIATFPANYASVRIICNIVTEHAKTINFSSRELYGIELATDEA
                     CANIIDHAYCGENMGSIHLFIETTDKEIKIVLEDAGKPFEPAEVLPPDLESPLEIRAE
                     RGLGVFLIKTLMDEVTFENPEPGLNRLTMIKHAKELATR"
     gene            2719..3387
                     /gene="radC"
                     /locus_tag="PK791_04125"
     CDS             2719..3387
                     /gene="radC"
                     /locus_tag="PK791_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOH92534.1"
                     /translation="MQNIDRPRERLLTDGVEVLSNAELLAILLGSGTKGQNAIELGAE
                     ILSSLGGFQGIHRHSIETLMSYKGVGEAKATRIKAAVELGNRMALEAIQVQPVISSPE
                     DVVRLVGHELRVKEQEELWVIRLDVRNRFLGLEKLYKGTQDSATVRVAEIYQTALRKG
                     AYAIMLVHNHPSGDPTESPEDVNLTRAVIEAAKYLDMYFLDHIIIGGTRFRSLKRQYP
                     SLWE"
     gene            complement(3347..>3843)
                     /locus_tag="PK791_04130"
     CDS             complement(3347..>3843)
                     /locus_tag="PK791_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOH92535.1"
                     /translation="AHRLLNQQFKDRLVNKIYHALVSPHPEWQSQNVNFPLAINTGRK
                     HLTRVDFINGKTAFSIFKVIKSSSRSCLLECDIKTGYRHQIRAHLYHLGIGILGDSLY
                     CPPQNPFPASIFSRMMLHARQIDFFHPRNQTKVSFIAQTPGIFFDLLDTAIPTDLDTD
                     ASMI"
BASE COUNT         1068 a          706 c          950 g         1119 t
ORIGIN      
        1 ggaagtcttg aacctgattg ttttgtattg cagacgctat ttgattagca atttcatgga
       61 taagttttag acgcttatct ttttctttta gttgacgcac gttaataatg agtaatccgt
      121 aattttcacc gggcacagtg agtggcgcaa tgacggtgtg aatctcgtct gtttttgatg
      181 attttgttga tggattgcga gagagtaatt gttgatacca agagtaaggt tttatgacag
      241 gtgaattaaa aacgtcgttt tctgacaagg tgtcaaatga caaggcaggc aactcattgg
      301 acaaatcatc aatattagct tgacttgaga ttatatgtgt acggacaata tgcgttccat
      361 cgtccttgcg ttgtagaatt aaaatatcct cagcgccaac aaagcatgtc aatgattgac
      421 ataagcggtc gagtgcgcat aaaagactgc cactggtcac caaataattg gatgtttgaa
      481 ggagtacgtt ggaaatgtag ctttcttcct ggcgttcatt gaccaaattg atgttctgta
      541 agttgatggc ggtttgctgg gcaatacctt gtaagatagt gaaacgttgt tttcccagca
      601 gatgtatcgg ctccgattgt tctgcgtcgg atttgctgat atgcatcaaa atgccaaggg
      661 tttggttgtg gacgacaagc ggaaacaaat aaacggtttc gttatcgcgc agtttgaaag
      721 cttttttaaa tttagatgaa aaagctgggg cgggaatggc tctttcaacc ggtttttggc
      781 aagtgtccca taaaagatca tcgtgaaaga tgatattaat gggatcgtcg tcttttttgg
      841 ggcttgtttt accgtaaact gcagccacgc tcagatactg tgtgtctgta caaggtagta
      901 aaagggcacc catacgtcca ccaacgagcg ctatggttag ttgcccgata gtttcaatga
      961 gttctggaat agtagtcaat gtttgcgttg ccagggcgac ctgtaagaga atcgtcgcaa
     1021 cccaggcctg gtcaattgat tgagcgctaa ggcgtgcgtt ttcgagagcg actgcggtat
     1081 agccggagaa cgaggcggca atattacggg cttcgttacc gtagcgtccg aagctgtttt
     1141 cgtgcaggat caatacgccg agttgtttgc caccggttga gagtggcgcg gcaatggcag
     1201 agaattcttc gtccaggtag ccatgttggt agaaagggtc agttttgtca ccggaaaggc
     1261 gaatgataac ctgatcttca tgaatggctt ttgaaaacca aacgtcatca atattaacgg
     1321 tgtaattttt cggctttttg atggtgtttg gctgggtacg gcttgaaact aatcggaatt
     1381 gagtgggttt ttgatcttct tcaagcgtat caaatatcca gatgcttgca attttactgg
     1441 gcagtaatgc catcattttt ttgaagacgg tttgaaaaag ttctttaagg ccaacatttg
     1501 tggcgagaag caaggcaata tcgcgcaggc tttccgcccc ttgccggcgc caaacttccg
     1561 aacggtacat gttagcgtta ttgatggcaa tagcaatata agcacttagg gtttcgagaa
     1621 cttcaacatc ctcaggcccg aaggcgtttt ctttatcact ttgcacgtct aaaacgccta
     1681 aaatctgatt gccatatatg agcggaacga caagctctga agatgtgaga gattcttcgg
     1741 gtcgatagag caagtattgc ggatcagttg aaactttgtt agatagttgc gttttaccag
     1801 tgcgcactgc gagagggata agtcctatgg gtgaatggat gtcgaaactg acttttactt
     1861 cattgtaaaa ctttgcgcgg gaaccactgc caaaggcaaa ttcaactgtt tgaggcacat
     1921 aatgcaccag ataaatatgt acaaagggat agccaaattc tttgtggacc agagacgtga
     1981 tacgcttcag cagacggttt aaatccaagg tctcagaaac agatttactg accttggcaa
     2041 taatttcaag ttttttttgc cgtgagtcgt tgcttacgcg gagcgtgtct gcctcacttt
     2101 tgaaattgag aatatcatca agggtctgct taggtcctgg atgatttttg gcgttcattt
     2161 actgtccagt atttgattat cgtgtggcta gctctttggc gtgcttgatc attgtcaagc
     2221 gattgaggcc tggctctgga ttttcaaaag tgacttcatc catgagtgtt tttatcaaga
     2281 aaacacctaa gccccgttca gcacggattt caagcgggct ttcaaggtcg ggcgggagta
     2341 cctcagcagg ttcaaatggt tttccagcat cttcgagaac gatttttatt tccttgtcgg
     2401 ttgtttctat gaaaaggtga attgagccca tattttcgcc acaataggca tgatcaatga
     2461 tattggcaca ggcttcatca gtggctaact ctatgccata tagctcacgc gaggaaaagt
     2521 tgattgtttt agcgtgctca gtaacaatgt tacaaataat tctcactgaa gcataattcg
     2581 caggaaatgt cgcaattttc attaatttac ctctcagaaa taatcgtcaa caatgaatta
     2641 tatcatgcct taaaaattaa acgctacagt ataattaata caatgaaaag agatggtaaa
     2701 acacgacgca taaatgatat gcaaaacata gaccggccac gggaacggct cctgactgat
     2761 ggcgtggaag tacttagtaa tgctgagtta ctggctattt tgttgggtag tggcacaaaa
     2821 ggccaaaatg caattgagct tggtgctgaa attctgtcca gtctgggggg atttcaaggt
     2881 attcaccgac acagtattga gactttgatg agttataaag gtgttggcga agccaaagca
     2941 acacgtataa aagcagctgt cgagctcgga aaccggatgg cacttgaagc tatccaagtc
     3001 caaccggtga ttagcagccc ggaagatgtt gtgcgactgg tcggacatga gttacgtgta
     3061 aaagaacaag aagaattatg ggttatccgc ctggatgtac gcaatcgctt tttaggactt
     3121 gaaaagttat ataaaggcac acaagattca gcgacggtgc gcgtagctga aatttatcaa
     3181 acggcattgc gtaagggcgc ttatgccatt atgttggtgc ataatcatcc atcaggtgac
     3241 cctactgaaa gtcctgaaga tgttaatctc acccgcgcgg taattgaagc cgcaaaatat
     3301 ttggatatgt actttttaga ccatatcatt attggcggaa cgcgttttag atcattgaag
     3361 cgtcagtatc caagtctgtg ggaatagcgg tatcaagaag atcgaaaaat atacctggtg
     3421 tttgcgcgat aaagctgacc ttggtttgat tgcgtgggtg gaaaaaatca atttgcctgg
     3481 cgtgaagcat cattcgcgag aaaattgatg caggaaaagg gttttgcggt ggacaataaa
     3541 gtgaatcacc aagaatacca atgcctaaat gatatagatg agcgcggatt tggtggcgat
     3601 accctgtttt aatgtcacat tccagtaaac aagaccgaga agaagattta atgactttaa
     3661 atatggagaa agcagttttt ccatttataa aatcaactcg cgtaaggtgt ttcctgccgg
     3721 tgtttatagc taaaggaaag ttaacatttt gagattgcca ttcagggtgc gggctgacta
     3781 aggcgtgata gattttattg acgagccggt ctttgaattg ctggtttaga agacggtgtg
     3841 cct
//
LOCUS       DAOSFC010000127         3813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_22042_length_3813_cov_4.655136, whole genome shotgun sequence.
ACCESSION   DAOSFC010000127 DAOSFC010000000
VERSION     DAOSFC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22042_length_3813_cov_4.655136"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PK791_04135"
     CDS             complement(<1..497)
                     /locus_tag="PK791_04135"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOH92536.1"
                     /translation="MDFTQLLAEVGKGFMAFGWKNAVMIVVALVLIYLAIAKDFEPVL
                     LLPIGFGALLANLGMSNQVGFMKVIYDAGIKTELFPLLIFIGVGAMIDFSPLLSQPSL
                     VLLGAAGQFGIYFTLLLAILLGFNLNDAASIGSIGAIDGPVAIFVANKLADPDLIAPI
                     AVAAYS"
     gene            complement(509..946)
                     /locus_tag="PK791_04140"
     CDS             complement(509..946)
                     /locus_tag="PK791_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOH92537.1"
                     /translation="MKIKVEIQEKIYEVTIHDIHARPVIAEVDGVQYQVWPEEQASAP
                     AVAAAAAPVVTPAAPEAAPAAPARAAVAAGASTLNAPLPGTIVAINVREGDSVEEGQE
                     LLTLEAMKMKNEIRADRAGVVARIHVNEGDLVKHGEALISWQG"
     gene            complement(946..1317)
                     /locus_tag="PK791_04145"
     CDS             complement(946..1317)
                     /locus_tag="PK791_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOH92538.1"
                     /translation="MIETFKQGLLITGIGMGLVFVMIFLLWGIMALLVWLTNPREKAS
                     DEPDADEAVELEPAALDVPAAQTVDDDEGQRLAAALAVAYALSRPSAQSFAPGVQTPQ
                     GSNWLSIGRAQQLLSNKRGRN"
     gene            complement(1327..2868)
                     /locus_tag="PK791_04150"
     CDS             complement(1327..2868)
                     /locus_tag="PK791_04150"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOH92539.1"
                     /translation="MTKKDPRLEKLQGLRKVAQLAGGQARIDRQHSKGSLTARERINL
                     LLDEGSFREIGALISHRGHTSDEPYGDGVVCGYGTVDGRTVYVYAQDFTIQGGSLGEM
                     HAEKICRVMDLAAKTGSPIVGMIDSGGARIQEGVYSLGGYAKVFRRNAIYSGVIPQIS
                     LIMGPCAGGASYSPAVTDLIIMVRGKSYMFLTGPAVIKTVTGEEVDSETLGGADVHLN
                     TSGNAHLVGDNDEDAIAIARRALSYLPSNNVDMAPVGDQSDDINRRAEALNTLVPIDP
                     SIPYSMHDAIEQIVDAGSFLELQKGFAGNAIVGFARLGGQPVAIVSQEPADRAGLMDV
                     DASDKIARMVNLADSFNLPIITFVDSPGFLPGTDQEYRGVIRHGAKVLYTYANATVPL
                     ISVVTRKAYGGAYVVLSSKYMGTDICYAWPSAEIAVMGAEGAANILYGRQIRESADPE
                     AKRAELTAQYQEEYLNPYEAAKAGFIDEVIEPAETRRKLIEALHALRNKVENTPPRKH
                     GNMPV"
     gene            3157..3681
                     /locus_tag="PK791_04155"
     CDS             3157..3681
                     /locus_tag="PK791_04155"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOH92540.1"
                     /translation="MNKELDQIVEDNTAVEMDYILTVDGEIADEGRLEYLQGHGNIIA
                     GLEKGLEGAKVGETRNVHVLAADAYGEHDPKRVLPVNRSSFPEDFEFKLGEPMRIRDA
                     SGLVFQAIPVALSEEIVELDLNHPMAGKDLDFEVTILNIRPATEAEIANGSLHFGGCA
                     GCSQGCDDPSTCGG"
BASE COUNT          979 a         1007 c          927 g          900 t
ORIGIN      
        1 gaataagccg ccaccgcaat cggcgctatt aagtccgggt ctgccagctt gttagccacg
       61 aaaatagcaa ccgggccatc aatcgcacca atcgagccaa tagaagccgc atcgttcaga
      121 ttgaagccca gcaaaatcgc cagcaaaagc gtgaaataga taccaaactg tcctgccgcg
      181 cccagcaaca ccaaactcgg ttgcgataaa agcggactaa agtcaatcat cgcgccaaca
      241 ccgatgaaga taagcaacgg gaagagttcc gttttgatgc cggcatcgta gatgactttc
      301 atgaaaccaa cctggttact catgcccagg ttggcgagca aagcaccaaa accaatcggc
      361 aaaagcaaaa ccggttcaaa gtctttggca atggcaagat aaatcagcac cagggcgacg
      421 acgatcatga ctgcattttt ccagccgaac gccatgaagc ccttgccaac ttcagccaat
      481 agttgcgtaa aatccattat caaccctcct aaccttgcca gcttattagg gcttcgccgt
      541 gtttaactaa atcaccttca ttaacatgaa tgcgtgccac gaccccagcg cggtcagcgc
      601 gaatttcgtt cttcatcttc atcgcttcca gggtcaaaag ttcctgacct tcttccacgc
      661 tatcgccttc gcgaacgtta attgccacga tggtacccgg taagggcgcg ttgagcgtgc
      721 ttgcgccagc agcaaccgct gcacgtgccg gggcagcagg agcagcctcg ggtgcagcag
      781 gagttacaac aggcgcagca gctgcagcga ctgcgggtgc gctggcttgt tcttcgggcc
      841 acacctgata ttgaacacca tcaacttcgg caatcaccgg tcgcgcgtga atgtcgtgaa
      901 tagttacttc atagattttt tcttgaattt caaccttaat tttcattagt ttcgtcctct
      961 tttatttgaa agcaattgtt gggcacgtcc gatgctcaac cagttggacc cttgcggggt
     1021 ctgaacgccg ggcgcaaaac tttgcgcgct ggggcgactt agcgcgtatg ccactgccaa
     1081 cgcggctgcc aaacgttgac cttcgtcgtc gtccacagtc tgtgcagccg gcacatccaa
     1141 agcagctggc tcaagttcaa cagcttcatc ggcatctggc tcgtctgaag ctttttcgcg
     1201 tgggttggtt aaccaaacca gcaaagccat gatgccccat aaaaggaaaa tcatgacaaa
     1261 gaccaagccc atgccgatac cggtaatcaa taagccttgt ttaaatgttt caatcattct
     1321 gcctccttag actggcatat tgccatgttt tcgtggcgga gtattttcaa ctttattgcg
     1381 cagggcatgc aaagcttcaa tcagcttacg gcgcgtttca gcgggttcga tgacctcatc
     1441 gataaagcca gccttggctg cttcgtaagg attgagatat tcttcctgat attgcgcggt
     1501 aagttcagcg cgtttggctt cggggtccgc ggattcgcgg atttgtctgc catacagaat
     1561 gttggcggca ccttcggcgc ccatcacagc gatttcagcg cttggccaag cataacaaat
     1621 atccgtaccc atatacttgg aactcagcac cacatacgcg ccaccataag ccttgcgggt
     1681 cacgacgcta atcaatggca ccgtcgcgtt ggcataggtg taaagcacct tggcgccatg
     1741 gcgaataacg ccacgatatt cctggtcggt tcctggcaaa aagccgggtg agtcaacaaa
     1801 ggtgatgatg ggcaaattga atgaatcggc taaattgacc atgcgcgcga ttttatcaga
     1861 tgcgtctacg tccatcagtc cagcgcgatc agctggttcc tgcgaaacga tggcaaccgg
     1921 ttgcccgccc aaacgcgcaa aaccaacgat agcattgccc gcgaaacctt tttgcagttc
     1981 aaggaaagaa cccgcgtcaa cgatctgttc aatcgcgtcg tgcatactgt aaggaatgct
     2041 ggggtctatc ggcaccagtg tgttcagcgc ttcagcgcgg cgattgatgt catccgactg
     2101 gtcaccaaca ggcgccatat ccacattgtt ggaaggcaaa tatgaaagag cgcggcgcgc
     2161 gatggcgatg gcgtcttcat cattgtcacc aaccaaatgc gcgttaccgc tggtattcaa
     2221 gtgcacatcc gcgccaccca gtgtttctga gtcaacttct tcgccggtca cggtcttgat
     2281 cacggcaggt ccggtcagga acatatagga tttaccacgc accatgatga taaggtcagt
     2341 taccgcgggt gaatagctgg cgccgccggc gcaaggaccc ataatcaggc tgatttgggg
     2401 aattacgcca gaataaatag cattgcgtcg gaaaactttt gcgtagccac ccagggaata
     2461 aacgccttcc tggatgcgcg cgccaccgga gtcgatcatg cccacaatgg gcgaaccggt
     2521 cttggcggca aggtccatca cacgacaaat cttctctgcg tgcatttcgc ccaaagaacc
     2581 accttgaatg gtaaagtcct gagcatagac gtaaaccgtg cgaccgtcaa ccgtgccata
     2641 accacacaca acaccgtcgc cataaggctc gtcgctggta tggccacgat ggctgataag
     2701 cgcgccgatt tctctaaagc tgccttcgtc aagcaaaagg tttatgcgct cacgggcggt
     2761 taaactgcct ttactgtgtt ggcggtcaat tcgggcctga ccaccagcaa gttgcgcaac
     2821 tttgcgtaag ccttgcaatt tctctaaacg gggatctttt ttagtcatgc aatctccttg
     2881 cgattaaaat attttattag tcatttgcca ttaagacgga taatggaccg tagctattgt
     2941 aacactgttt gagcggtttt tggcctttaa cttttagata ttcagcataa agccctgtgc
     3001 gtttttgaag aaatcttttc gtatatggac aacagccata aaccgcttag gaaaatgtca
     3061 aatatcctga tgcaagtccg tgatagactg caaagtttct gttacaatta cgcggcttga
     3121 gcaacgataa aacccgctca acttgaatgg agacaaatga ataaagaatt agatcaaatt
     3181 gttgaagata acactgccgt tgaaatggat tacatcctaa ccgttgatgg cgaaattgcc
     3241 gacgaaggcc ggcttgaata tctgcagggc cacggcaaca tcatcgctgg tcttgaaaaa
     3301 ggtcttgaag gcgcgaaagt tggcgaaacc cgcaacgtgc acgttttagc cgccgacgcc
     3361 tatggcgaac acgaccccaa acgtgtcttg cccgtcaacc gcagttcctt ccccgaagat
     3421 tttgagttta aattaggcga accgatgcgc attcgcgatg ccagcggact ggtttttcag
     3481 gccattcctg tagcactcag cgaagaaatc gttgaactcg acctgaacca ccccatggcc
     3541 ggcaaggatt tggatttcga agtgactatc ctgaacatcc gccccgcgac ggaagcagaa
     3601 attgccaatg gcagtttgca ctttggcggt tgcgcgggtt gcagtcaggg ttgcgatgac
     3661 cccagcacct gtggcggata aaacatccca aagttaaaaa caaaaagaga ctgtcgccat
     3721 gatgttgaaa cagtctcttt tttaaatagt ttttaggggc tattctttgt ttggtgtgga
     3781 tgcctgaacg atcgcgctaa tcaagccaaa caa
//
LOCUS       DAOSFC010000128         3792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_22237_length_3792_cov_5.002141, whole genome shotgun sequence.
ACCESSION   DAOSFC010000128 DAOSFC010000000
VERSION     DAOSFC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22237_length_3792_cov_5.002141"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PK791_04160"
     CDS             <1..1041
                     /locus_tag="PK791_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOH92541.1"
                     /translation="GMKYICFTAKHHDGFALFNTQVSDFNSVKASPCQRDFVAELADA
                     CRQEGLKFCLYYSQAQDWHHAGGYRAYQHDEKQNDAQAFEAYFESICLPQVRELLSHY
                     GEISMLWFDTPMGMTLKQSQKLVALVKSLQADCLINGRIGHGLGDYLSMADNRIPRGA
                     IKSAWEVPVTLNQSWGYKASDEAWTSPAELIKKWLKIISRSGNILLNVGPRGDGSLPE
                     DSTTILDTIGAFLTRNAEAFYHTLPTPEYVFELDGVYFTHKPGLLYITVLEPKKWTGK
                     WLSLYHMKSIASQASLLASGDTLELRVGKDLEGFGHWVIRLPETLYAEDKLGWVIKVT
                     LESENFEVEALE"
     gene            1185..1412
                     /locus_tag="PK791_04165"
     CDS             1185..1412
                     /locus_tag="PK791_04165"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOH92542.1"
                     /translation="MSRYVVITSDKKKITALLLCIFLGVFGAHQFYVGKIGKGLLYLF
                     TVGLFGIGWIVDIIKILTGSFRDNVGAPLRQ"
     gene            1476..2753
                     /gene="serS"
                     /locus_tag="PK791_04170"
     CDS             1476..2753
                     /gene="serS"
                     /locus_tag="PK791_04170"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOH92543.1"
                     /translation="MLDIKLIRENPEIVREALQKRHMEIEVVDEVIALDERRRAIILD
                     VEGKKAERNIASKEIGKMKDKEAREAKIAATRELGDEIARLDEELKGVEEALYAAVAG
                     IPNIPDASVPYGADDKENVVLQQFGEKKVFDFEPKPHWDLGPDLGIIDFEAGVKLAGS
                     RFYVLKGAGARLERALIFWMLDLHLRRGYKEIYTPWMVRGEVMFASGQLPKFEANLYH
                     DHEENFWWVPTAEVPLTGMMMDTILDEADLPMNLTAYSACFRREKMSAGRDVRGIKRG
                     HQFDKVELYKYCTPENAEIEFENMLADATEVCKQLGLTYRTMQQCTGDLGFNAHKTVD
                     VEVWAPGCGEWLEVSSVSNVGQFQARRAAIKYRAADGSGTQFVNTLNGSGLGLPRTLI
                     AVLEQYQQADGSIVVPEVLLPFMGGIERIESGM"
     gene            2824..2905
                     /locus_tag="PK791_04175"
     tRNA            2824..2905
                     /locus_tag="PK791_04175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2858..2860,aa:Leu,seq:caa)
     gene            3038..>3792
                     /locus_tag="PK791_04180"
     CDS             3038..>3792
                     /locus_tag="PK791_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH92544.1"
                     /translation="MESKQERATKHYLIIGGGIAGIATAETIAKNEPGAKITVVCEET
                     LLPYFRINLTMYLAGNMEAKRLTMHPAEWYTDRNINLLLNTVAERVDPQKRTVHLRDG
                     SQLAYDTLILANGASPFVPPIKGVELDGVKTLRTIADADAILEASQHAAKVVCIGGGL
                     LGLEVAGAIAESGGEVVVLEAQPWLLPRQLDERASQHLQRRIEAQNVRVLLNVQTEAI
                     LGDGRVEKVRLADGRELPADLVVISAGVRPNLDL"
BASE COUNT         1023 a          916 c          990 g          863 t
ORIGIN      
        1 ggtatgaaat acatttgttt cactgccaaa catcatgacg gtttcgcgct ttttaacaca
       61 caagtttcag attttaatag cgtaaaagcc agtccatgcc agcgcgattt tgtcgccgaa
      121 ctcgccgatg cctgtcgcca ggaaggactc aagttttgcc tttattattc acaggcgcaa
      181 gactggcatc acgcgggcgg ttaccgcgcc taccaacacg acgaaaagca aaacgacgcg
      241 caagcttttg aagcctactt tgaatccatt tgcctgcccc aggtgcgtga gcttttgagc
      301 cattacggtg agattagcat gctttggttc gacacaccta tgggcatgac actcaaacag
      361 agtcaaaaac tggtcgcgct ggtgaagtca ctccaggcag actgcctgat taacggacgc
      421 attgggcatg ggctgggtga ttatctcagc atggcggata atcgcattcc gcgcggagca
      481 attaaaagcg cctgggaagt gccagttaca ttaaaccagt cctggggcta taaagcgtcg
      541 gacgaagcct ggacaagccc agcggagctg atcaaaaagt ggctaaaaat catcagtcgt
      601 agcggaaaca ttttgctgaa cgtcggccca cgtggcgacg ggagcctgcc cgaagattca
      661 acgacaatcc tggacaccat cggtgctttt ctgacaagaa acgccgaagc tttttatcat
      721 actctgccca cgccagagta tgtgtttgaa cttgatggcg tctatttcac gcacaagccg
      781 ggcctgttgt atatcaccgt cctggaacct aaaaagtgga caggcaaatg gctttccttg
      841 taccacatga agtctatcgc ttcccaagcc agtctgctgg ccagcggaga caccctggaa
      901 ttgcgcgttg gcaaagacct tgaaggcttc ggacactggg ttatccgctt gcctgaaacc
      961 ctttatgctg aagataagct gggctgggtg attaaggtaa cgcttgaaag cgaaaacttt
     1021 gaagtagaag ccctggaata gtgccccttg caaacaagaa cacatacaga ttgcgtgttt
     1081 cctgaataaa acgaaggtat aagccagttt cgtgattatg cctttaatca gccctggcag
     1141 tggtagaatt gctttgcaag aaaaaataaa aggaagtaca aaaaatgagc agatatgttg
     1201 taatcaccag tgataagaaa aagataacag ccttgttact gtgcatattt cttggcgtct
     1261 ttggcgcgca tcagttttat gttggcaaga ttggcaaagg tttgttgtat ttatttactg
     1321 ttggtctctt tggaatcggc tggattgtgg atatcatcaa aatcctgacc ggcagcttta
     1381 gagataacgt tggcgcgcca ctcaggcagt aaacacaact ctgctttgca acattttaga
     1441 ctcaataaca aaataaaaat cagaaagaaa acatcatgct tgatatcaaa ttaatccgtg
     1501 aaaacccaga aatcgtgcgc gaagcactgc aaaaacgaca catggagatt gaagtcgttg
     1561 atgaagtcat cgcgctggac gaacgccgcc gcgccatcat cctggacgtt gaaggcaaaa
     1621 aagcagaacg caacattgcc agcaaagaaa ttggcaagat gaaagacaag gaagcccgcg
     1681 aagcaaaaat cgccgcgaca cgcgaacttg gcgacgaaat cgcgcgtctt gacgaggagt
     1741 tgaaaggggt tgaagaagcg ctttacgccg ccgtggcggg cattcccaac atccccgatg
     1801 ccagcgtgcc ctatggcgcg gacgataaag aaaacgtcgt tttgcaacag tttggcgaga
     1861 aaaaggtctt tgattttgaa cccaagcccc actgggattt gggccccgac ttgggcatca
     1921 tcgactttga agccggcgta aaactggcgg ggtcgcgttt ttatgtgctt aaaggtgccg
     1981 gggctaggct cgagcgcgcg cttatcttct ggatgctgga tttgcatctg cgccgcggtt
     2041 acaaggaaat ttacacgccc tggatggttc gcggggaagt tatgttcgct tctgggcaac
     2101 ttcctaagtt tgaagcaaac ttatatcacg atcacgaaga aaacttttgg tgggtaccca
     2161 ccgccgaagt gcctttgacc ggcatgatga tggataccat cctggatgaa gcagacttac
     2221 cgatgaacct gaccgcttac agcgcctgtt tcagacgcga aaagatgagc gccggacgcg
     2281 acgtgcgtgg tattaaacgc gggcaccagt ttgacaaggt cgaactttac aaatactgca
     2341 cgccggaaaa cgctgaaatt gaatttgaaa acatgctggc agacgccacc gaagtctgca
     2401 aacaactcgg tttaacctac cgcaccatgc aacagtgcac gggcgacctg ggctttaacg
     2461 cgcacaaaac agttgacgtt gaagtttggg cgccgggctg tggcgagtgg ctggaagtgt
     2521 cttcggtgtc gaatgttgga cagttccagg cgcgtcgggc ggcgattaaa taccgcgcgg
     2581 cagatggcag tggcacacaa tttgtgaaca ctctcaacgg ctccggtctg ggcctgccac
     2641 gcactttgat tgcggtgctt gagcaatatc aacaggctga tggcagcatt gtggttccgg
     2701 aagtgttgtt acccttcatg ggtggtattg agcgcataga atcaggaatg tagcttgaga
     2761 cattcgaaat taatgcgaaa tacttgacca caaacgccat ttgagagtat actcttttca
     2821 ctcgccgaag tggcggaata ggcagacgcg cgcgactcaa aatcgcgtac ctacgggtat
     2881 gtgggttcga ttcccacctt cggcattcca cgcaagcaaa cttgcgtggt tttcttttaa
     2941 atcattagaa agctaacaaa gcatccacag acatcaatta tctactggca ctatgctaaa
     3001 ataatctcat caagattctg ataaaaagag gtgcaccatg gaaagcaagc aagaaagagc
     3061 cacaaaacat taccttatca tcggtggagg tatcgcaggc atagcaactg cagaaaccat
     3121 cgccaaaaat gaaccaggcg ccaaaatcac cgtggtctgc gaagaaacgc ttctgcccta
     3181 tttccgcatc aacctgacca tgtacctggc tggcaacatg gaagccaagc gccttaccat
     3241 gcaccccgcg gaatggtaca ccgacagaaa tatcaatctc ttgctgaata ctgttgccga
     3301 gcgcgttgac ccgcaaaagc gaaccgttca cttgcgcgat ggcagccaac tggcatatga
     3361 cacactcatt ttagcaaatg gtgcatcacc ttttgtgccc cccatcaaag gtgtggaact
     3421 tgatggcgta aaaaccctgc gcacaattgc cgacgcggac gccatcctgg aagcaagcca
     3481 acacgccgcg aaagtggttt gcatcggtgg tggactgctt gggcttgaag ttgccggcgc
     3541 gattgccgaa agcggtggcg aggttgtggt gcttgaggca caaccctggt tattgccccg
     3601 tcagttggac gaacgcgcca gccagcattt gcagcgtcgc attgaagcac agaacgtgcg
     3661 tgttttgctg aatgtgcaaa ctgaagcgat tcttggcgac gggcgcgttg aaaaggtgcg
     3721 cctggcggat ggacgggaac tgccggcaga tttggtggtc atcagcgcgg gtgtcaggcc
     3781 taatcttgat ct
//
LOCUS       DAOSFC010000129         3775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_22394_length_3775_cov_4.061828, whole genome shotgun sequence.
ACCESSION   DAOSFC010000129 DAOSFC010000000
VERSION     DAOSFC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22394_length_3775_cov_4.061828"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK791_04185"
     CDS             <1..388
                     /locus_tag="PK791_04185"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOH92545.1"
                     /translation="INLIISLIIYAYAFFVKDFSVMIYSLIFTLIYAFVVDLTHYQNV
                     KISLTIFTKQEDVLDIITKDLGRGATYWQGKGGYSGQDTFVINTVVSKYELIRLRRHI
                     QERDPNAFIVESTDVHVTGKFLSNFF"
     gene            394..1140
                     /locus_tag="PK791_04190"
     CDS             394..1140
                     /locus_tag="PK791_04190"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOH92546.1"
                     /translation="MSNKVLVEICCGSAEDAIEAWHGGADRVELCSALFLGGLTPSLG
                     SLLELKAQAEIKVAAIIRPRQGGFCYSESDFRAAMHDAEQMLKHGADALVFGFLHADG
                     RVDLARTRAMVELAGDVPVCFHRAIDVVPDWREAFDGLMEIGVQRVLSSGQASSAYEG
                     RYVLREMMDYAAGRLQVMPGGGIRAGNVHKLLAVTGAREIHLGGVSRLMHDSSTQHNP
                     GLRFGDPKSPPESEYKRVSHDAVAEFIASL"
     gene            complement(1151..2221)
                     /locus_tag="PK791_04195"
     CDS             complement(1151..2221)
                     /locus_tag="PK791_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOH92547.1"
                     /translation="MKTISDGLTFDDVLILPGYSEILPRDVDIHVQLTRDIRLEIPLI
                     SAGMDTVTEADMARAIAQEGGLGIIHKNMSIAAQAEQVARVKSENLLVGAAVGVTHDV
                     LDRVSALVQAGVDIITVDTAHGHSKGVLDTVRKIKEAFPSLPLIAGNVATAEGTLALI
                     DDGADVIKVGIGPGSICTTRVVTGIGVPQITAVMECARVARERGIAIIADGGIRLSGD
                     ITKALAVGANAVMLGSLFAGTDESPGQVIRVNGKDYKSYRGMGSLGAMDSGSADRYFQ
                     SESKKYVPEGIEGTVLYKGAMKDVVYQLMGGLRSGMGYVGAANLTELYEKSKLIKITQ
                     ASLIENHPHDIEMIKAAPNYTP"
     gene            2420..2950
                     /locus_tag="PK791_04200"
     CDS             2420..2950
                     /locus_tag="PK791_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH92548.1"
                     /translation="MEIFEVLKRRRSTRQYTGKAVSQEDLELILDAAMLSPVARARFE
                     DMHLTVISDKTLLQAISENVRQKTGRDRDPLFGAPVLVLVSVKPAGEVPQNNEYSSAA
                     MIAHNMTLAATALGLGSCDIWGAIGLANQNPELKARFGLPEGFVAVCSVTIGETDEAM
                     PVRDVDKQKIAKHYIA"
     gene            3124..>3775
                     /locus_tag="PK791_04205"
     CDS             3124..>3775
                     /locus_tag="PK791_04205"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOH92549.1"
                     /translation="MKKYQKTTQIIFLLAILLISLISPLQSAQAQSTDVLPSGLPVSQ
                     LATAIEDHVQENQATTAGMAVGVFDGDEILYENYFGFANKAENLHVDADTVFEWGSVT
                     KLITWVSVMQVWEQGLLDLNADIRTYLPDTLLSDLKYDEPITMIHLMNHTPGFEEVYK
                     NIFANEADAAKNLETLLVESEPRQIFPPGELTAYSNWGATLAGYIVERVSGQNFADY"
BASE COUNT          916 a          873 c          990 g          996 t
ORIGIN      
        1 tatcaatttg attatcagcc tgattatcta tgcctacgct ttctttgtga aagactttag
       61 cgtgatgatt tactcgctta tcttcacctt aatctacgcc tttgtggtcg atttaaccca
      121 ctatcaaaat gtgaagatta gcttgacaat attcacaaaa caggaagacg ttttagatat
      181 catcaccaaa gatcttgggc gtggtgctac ctactggcag ggtaaaggtg gttattccgg
      241 acaggacacc tttgtgatta acacggtggt atcgaagtat gagctgattc gcttaaggcg
      301 tcatattcag gaacgtgacc ccaacgcgtt catcgttgaa agtaccgatg tgcacgtgac
      361 cgggaagttt ttgtccaact ttttctagtg caaatgtcaa acaaagtgct tgttgaaatc
      421 tgctgtggtt cggcggaaga tgccattgaa gcctggcatg gtggcgcgga ccgcgtggaa
      481 ttgtgctcgg cactcttttt gggcggttta acgccatcgc tggggtcgtt actggaacta
      541 aaagcacaag cagagattaa agtcgcggcg attatccgtc cccgccaggg cggtttttgc
      601 tattctgaaa gtgattttag ggcggcgatg cacgacgcgg agcagatgct gaaacacggc
      661 gcggatgctt tggtgttcgg ttttttgcat gcggacggtc gcgttgactt ggcgcgcacg
      721 cgggccatgg tcgaactggc tggtgacgtg ccggtgtgtt ttcaccgcgc gattgatgtt
      781 gtgcctgact ggcgggaagc gtttgatggc ttaatggaaa tcggtgtgca acgtgtttta
      841 agcagtgggc aggcctcatc ggcttatgaa gggcgctatg tcttacgcga gatgatggac
      901 tatgctgcgg gtcgtctgca ggtcatgcct ggcgggggaa ttcgcgctgg gaatgtgcat
      961 aagcttttag ccgtaacagg cgcaagggaa attcacctgg gtggtgtttc gcgcctgatg
     1021 cacgacagtt ccacgcaaca taatcctggc ttgcgttttg gtgacccaaa aagcccgccg
     1081 gaaagcgaat ataagcgcgt cagccacgac gcggtagctg agtttatcgc cagtttgtaa
     1141 tcagaacgct ttagggcgtg tagttagggg cggctttaat catctcaatg tcatgcggat
     1201 ggttttctat caggctggct tgggtgattt taatcagttt ggatttttca taaagttccg
     1261 tcaggtttgc cgcgccgaca tagcccatgc ccgaacgcaa gccacccatc agctgataaa
     1321 cgacatcttt catcgcgcct ttatagagta ccgtgccttc aatcccttcg ggaacgtact
     1381 ttttgctttc ggactggaag tagcggtcgg cactgccact gtccatcgcg cccagggaac
     1441 ccatgccacg atagctttta taatctttac cgtttacgcg gatgacctgg ccgggacttt
     1501 cgtccgtgcc ggcaaaaagc gagcccagca tgaccgcgtt cgcgccaact gccagggctt
     1561 tggtgatgtc gccggaaagc cgaatgccac catcagcgat gatggcgatg cctcgttcgc
     1621 gggcaacacg cgcgcattcc attaccgcgg taatttgggg cacgccgatg ccagtgacca
     1681 cgcgggtggt gcaaatggaa ccggggccga tgcctacttt gatgacgtcc gcgccgtcgt
     1741 cgatcagcgc cagggtgcct tccgcggtgg cgacgttgcc ggcgatgagt ggcaggctgg
     1801 gaaaggcctc tttgattttg cgtacggtgt ccagcacgcc tttggaatgg ccatgcgcgg
     1861 tatcaacggt gatgatatcc acaccggcct gaaccaaagc gctgacccgg tcgagcacgt
     1921 cgtgggtcac gccaaccgcc gcgccaacca ggagattttc ggattttacg cgggcaactt
     1981 gttcggcctg ggcagcaata ctcatgttct tatggataat gcccaaacca ccttcctgtg
     2041 cgatagcgcg tgccatgtct gcttcggtga cagtatccat acctgcgctg atgagcggga
     2101 tttcaaggcg aatgtccctg gtgagctgga catgaatgtc tacgtcacgg ggcagaattt
     2161 cggagtaccc gggaagaatg agaacatcat caaaagtcaa tccgtcagat atggttttca
     2221 tggcaaatcc tttgtttcgc gaaagtttta tctgcataaa tcttaccata aactgccttt
     2281 ttttcagtaa aaacagggcg aatagcaacc gtgtttggca ttgtttatcc ccgcgtttca
     2341 tgtcccccga attttctaat aaaaatttat ctgttcacat gttatcataa acttaacaga
     2401 aaaaagcaaa ggagaatata tggaaatttt tgaagtcttg aagagacgac gatctacccg
     2461 tcaatatact ggaaaagctg tcagccagga agatttggaa cttattttgg atgcagctat
     2521 gctgtcgccg gttgcccgcg cgcgttttga agatatgcat cttaccgtca ttagcgataa
     2581 aactttattg caggctatct ctgaaaatgt ccgccaaaag accggacgag atagagaccc
     2641 gctctttggc gcgccagtct tggtgctggt atctgttaaa ccagctggcg aggtgcccca
     2701 aaacaacgaa tactccagcg cggcaatgat tgcgcataac atgacccttg ccgcgaccgc
     2761 tttgggtttg ggaagctgcg atatttgggg cgcgattggg ctggctaatc aaaatcctga
     2821 actaaaagcg cgctttggct tgcccgaagg ctttgtggcg gtgtgttcgg tgacgatcgg
     2881 cgaaactgat gaagcaatgc ctgttcgtga tgttgataaa caaaaaattg ccaagcacta
     2941 catcgcataa gctgtttatc taagttaatt tccaagtaaa aaacagggtt ttcaatatca
     3001 gaagaccctg ttttgtattc cagggtggga atttgttagt tggcaaacat aagtataggt
     3061 atgctttaca gctattttac agtcatgaaa caagatgtta tcgcgtttcg ctataattaa
     3121 tctatgaaaa aatatcaaaa aacaactcaa atcatctttc ttttggcgat tttattaata
     3181 tcgcttatct ccccattgca aagtgcccaa gcgcaaagca cagacgtttt gccatctggt
     3241 ctgcccgttt cccagctggc aacggcgatt gaagaccatg tccaggaaaa tcaagccacg
     3301 accgcaggta tggcagttgg cgtttttgac ggcgatgaga ttctgtacga aaattatttt
     3361 ggttttgcga acaaggctga gaacttacac gttgacgctg atactgtctt cgagtggggc
     3421 agtgtgacca agctcattac ctgggtaagc gtgatgcaag tctgggaaca aggattgctg
     3481 gatctcaacg cggatatcag aacctatctg cctgacacac ttctctctga tctaaaatat
     3541 gacgaaccga ttacgatgat tcatctgatg aatcatacgc ctggatttga agaagtgtat
     3601 aaaaatattt ttgccaatga agcagacgct gccaaaaacc tggagacctt gctggtcgaa
     3661 agcgaaccca gacaaatctt tccgcctgga gagcttaccg cttattccaa ctggggtgcg
     3721 actttggcag gctatattgt tgaacgcgta agcggacaaa actttgctga ttatg
//
LOCUS       DAOSFC010000130         3772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_22424_length_3772_cov_5.644337, whole genome shotgun sequence.
ACCESSION   DAOSFC010000130 DAOSFC010000000
VERSION     DAOSFC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22424_length_3772_cov_5.644337"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..96
                     /locus_tag="PK791_04210"
     tRNA            9..96
                     /locus_tag="PK791_04210"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Ser,seq:cga)
     gene            complement(231..3209)
                     /locus_tag="PK791_04215"
     CDS             complement(231..3209)
                     /locus_tag="PK791_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012027.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HOH92550.1"
                     /translation="MELILQQGLPHQQKAIDKIRQVFDSVDFFRPQIFYENPSLDLAD
                     SRLKQNIEAIQRDIPAEYCNHEPVGDCLTLDIKMETGTGKTYVYTQTMFELHKHYGIN
                     KFIIAVPSLAIKAGTAQFLLDPYARRHFSDSCGYGTTIELGVLTAQTRARNKRSYFPG
                     VVSDFVKGSFQNTRKIYVLLTNMHLLTTRKNAMLARDDYDYGVEGFYRPLDAIQATRP
                     FVIIDEPHRFARDGVSYKVLAEEIEPQCIIRFGATFPETSKGRGKNKVVKKDYQNLLY
                     DLNAAASFNQNLIKGVAKEHLEPVSEQDAKVKIVSLKSRVSANFQYKAKDKPTKTYTL
                     QKDDSLAIIDTAFEGIRLDGISNSEVVFSNGQSKTLGEELDVDIYMSSYQEQMLRLAL
                     ERHFETEKENFCNRQYKIKTLALFFIDDISSYRANEEGKTPYLLEAFERLLKEKLNQE
                     IQNLTEHETAYKDYLQASLNELSACHAGYFSQDNTDSDEGIAEEVQEILHGKKELLSF
                     KRKDGSWNTRRFLFSKWTLKEGWDNPNVFTIAKLRSSGSEISKLQEVGRGLRLPVDEN
                     GNRISNEEFWLNYIVDFTEFDFAEELVKQINGELPQAAKLTEEMLSKLAKKRGIDADA
                     MFDELYSKKFIDRHMNINNEYREAMWEEYPSLELGLQKGKVKDRNKQKKETIKIRKAV
                     FQEIRELWERINERYLLLYDEDLDKELPDVLLKLLEENNIFTQVSVTSQREKVVSRGG
                     RMHVQQETGRQYIVSRPLPYGEFLMRINKATNLPVALVHQALSEHQKRHGDLRPDHFN
                     EKAVSAFCASFQAWKTKSLQGRFSYSRSKSSGGATALTRADGTVLDEIAQGRIGTKIA
                     EGTPSDKYLYDTFAYDSALEQKNLMADIDEVLVYGKIPRSSIAIPTVTGGTYSPDFMY
                     IIKRASGEKELNLIVETKDTENKSELRETEAAKIECARVFFENLSLEGYKVYFRNQLN
                     NRQMLEIVKSVIEEDDKKAK"
     gene            complement(3225..>3772)
                     /locus_tag="PK791_04220"
     CDS             complement(3225..>3772)
                     /locus_tag="PK791_04220"
                     /inference="COORDINATES: protein motif:HMM:NF036754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92551.1"
                     /translation="EDKPAYKAGYRSIDEIGRERIIRAAKKIKEEKPDTTADLGFKHF
                     VLAEPSGKQLDEIIDFDHKVNVVVTNTLLQEFGTDAILATWLVRDGYGFSPELKTVKF
                     GDYTAYYIDKHLYLINELADYSALEAMVTRFDTDPSFNPENIVLFSYSFTWTQLEMIQ
                     TNLKRLKATEKNLRLNFDVRY"
BASE COUNT          926 a          863 c          798 g         1185 t
ORIGIN      
        1 ttgaacctgg agagatgccg gagtggttga tcggggcggt ctcgaaaacc gttgtgggac
       61 ttgtgcccac cgtgggttcg aatcccactc tctccgctga tgtagcttgg ggaggtttaa
      121 ataaagctat tcttttaaca agtctcgacc agtttcatta aatcttcaag cgcccgtttt
      181 actgtgcaaa agttaagtgt gttttttata catcttggtc acttacttgg tcattttgcc
      241 tttttatcat cttcctctat gacgcttttc acaatctcca acatttgtct gttattcagt
      301 tgattcctga aatatacctt gtagccttcc aaagacaagt tttcaaagaa cacccgggcg
      361 cattcaattt tcgcggcttc tgtttcccgt aattcagact tgttttctgt gtcctttgtt
      421 tccacgatga gattcagctc tttttctcca gatgcacgtt tgatgatgta cataaagtcg
      481 ggactatagg tgccacccgt aactgtggga atggcgatac tgcttctggg tatcttccca
      541 taaacaagca cctcgtcaat atccgccatg agattcttct gctccagggc tgagtcatat
      601 gcgaaggtat cgtagagata tttatcgcta ggtgttcctt ccgcgatttt tgttcctatt
      661 cgcccttgcg caatttcatc cagcaccgtg ccgtcggctc tggttaatgc ggtggcaccg
      721 ccactgcttt tagacctcga ataagaaaaa cgcccttgca gggattttgt tttccatgcc
      781 tgaaatgacg cgcaaaaagc tgacaccgct ttttcattaa aatggtctgg tcttaaatca
      841 ccatgtcgct tttgatgctc gctcaaagcc tgatgaacaa gcgcgaccgg taaattggta
      901 gctttattaa tgcgcataag aaactccccg taaggaaggg gacgactaac aatatactgt
      961 ctgccggtct cctgctgaac gtgcattctg ccaccgcgcg atacaacttt ttcgcgctga
     1021 ctcgttacag aaacctgggt aaaaatgttg ttttcttcca ggagttttaa aagaacgtct
     1081 ggcaattctt tatccagatc ctcatcatac agtaaaagat aacgctcgtt aatcctttcc
     1141 caaagttcac gtatttcctg aaagacagct ttcctgattt taatcgtttc ttttttctgt
     1201 ttattgcggt ctttgacttt gcccttttgc aagcctaatt caaggctggg atattcttcc
     1261 cacatcgctt cgcggtactc attgttgatg ttcatatggc ggtcaataaa cttcttacta
     1321 taaagctcat caaacatcgc gtcggcatca ataccccgct ttttagccag tttggaaagc
     1381 atttcttctg taagctttgc cgcctgggga agttcgccat taatctgttt aaccagttct
     1441 tcggcaaaat cgaattctgt aaagtcaaca atatagttaa gccaaaattc ttcattggat
     1501 atgcggttgc cattctcgtc aaccggcagt ctcaaacctc tgccgacttc ctgtaatttg
     1561 ctgatttcac ttccgctcga acgcagttta gcgatagtaa aaacattggg gttatcccag
     1621 ccttctttta gtgtccactt ggaaaaaaga aaacggcggg tattccacga accatcttta
     1681 cgcttgaagg ataaaagttc ttttttacca tgtaaaattt cctgcacttc ctcagcgatg
     1741 ccctcatcag aatcggtatt gtcctgtgaa aaatatccgg catggcaggc agaaagctca
     1801 ttcaagctgg cttgcaggta gtctttatac gctgtttcat gttctgttaa gttttgtatt
     1861 tcctggttta gtttttcttt aagcaaacgc tcgaaagctt ccaaaagata tggggtttta
     1921 ccttcttcat tcgcccgata agagctgata tcgtcaatga aaaacagagc cagggttttt
     1981 attttgtatt gtcggttaca aaagttttct ttttcggttt caaagtgtct ttccagtgcc
     2041 aggcgtaaca tttgttcctg gtaagaactc atgtaaatat caacatccaa ctcttctccc
     2101 aaagtttttg actgcccatt tgaaaaaaca acctcactgt tgctgatgcc atcaagccta
     2161 atgccttcaa aagccgtgtc gatgatggca agcgaatcat ctttttgcaa agtgtaggtt
     2221 tttgtgggtt tgtctttggc tttgtattga aaattagcag agactctgct cttgagcgat
     2281 acaattttga ccttggcatc ttgttccgaa accggctcca gatgttcttt agccacccct
     2341 ttaatcaagt tctgattaaa cgaagcggcg gcattcaaat cataaagtaa gttttggtag
     2401 tcctttttga cgaccttatt tttaccccga ccttttgatg tctccggaaa agttgcgcca
     2461 aagcggatga tacattgcgg ttcgatttct tcagcgagta ctttatatga gaccccgtct
     2521 ctcgcaaaac gatggggttc atcaataatc acaaaaggcc gtgtcgcctg gatcgcgtcc
     2581 aaaggcctgt aaaaaccctc aacgccatag tcatagtcat cgcgcgcgag catggcgttt
     2641 ttgcgcgtcg tcaaaaggtg catattggtc agcagaacat aaatcttcct ggtgttttgg
     2701 aacgagcctt taacaaaatc gctgaccaca cccggaaaat aagagcgttt gtttcttgcc
     2761 ctcgtttgcg cggtcagcac acccagttca atcgttgtac catagccaca actgtccgaa
     2821 aaatggcgac gcgcgtaggg gtcaagcaag aactgagctg tgccggcttt gatggccagc
     2881 gatggcacgg caataatgaa tttgttgatg ccataatgct tatggagttc aaacatggtt
     2941 tgggtataga cataagtttt gccggtgccg gtttccattt tgatatccag cgtcaggcag
     3001 tcgccaacgg gctcatggtt gcaatattct gcaggtatat cgcgttgtat tgcttcaatg
     3061 ttttgcttca ggcgcgaatc agctaaatca aggcttgggt tctcataaaa gatttgtggc
     3121 ctaaagaaat caacactgtc aaagacctgc ctaatcttgt caatagcttt ctgctgatgg
     3181 ggtagcccct gctgtagaat taattccatg ctataaactc ctcatcaata gcgaacatca
     3241 aagttaagtc gcaggttctt ttccgtcgct tttaagcgtt tgagattggt ctgtatcatt
     3301 tccagttgtg tccaggtaaa actataacta aatagcacaa tgttttccgg attaaaagat
     3361 gggtcagtat caaatcttgt gaccattgcc tccagggcac tgtagtcagc taattcgttg
     3421 atgagataca gatgcttgtc gatgtagtaa gcggtatagt cgccaaattt aacggtcttg
     3481 agttccggtg aaaaaccata gccatcgcgc accagccagg tagccagaat ggcatctgtg
     3541 ccaaattcct gcaagagcgt atttgtaacc actacattta ccttatggtc aaaatcgatg
     3601 atttcatcaa gctgttttcc gcttggttct gccaacacaa agtgtttaaa accaaggtcg
     3661 gctgtggtat cgggcttttc ttctttgatt ttcttcgcgg cgcggatgat gcgttccctg
     3721 ccgatttcat caatactgcg atagccggct ttgtaggcgg gtttgtcttc tt
//
LOCUS       DAOSFC010000131         3755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_22572_length_3755_cov_5.164324, whole genome shotgun sequence.
ACCESSION   DAOSFC010000131 DAOSFC010000000
VERSION     DAOSFC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22572_length_3755_cov_5.164324"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PK791_04225"
     CDS             complement(<1..1115)
                     /locus_tag="PK791_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOH92552.1"
                     /translation="MQFDLQAPYKPTGDQPQAIADLVKGVRQGYRHQVLLGATGTGKT
                     FTVANVIQQLQLPTLVMTHNKTLAAQLYAEYKDFFPNNAVEYFVSYYDYYQPEAYIPR
                     RDLYIEKETQINEEIDRLRLAATTALMSRQDVIIVASVSAIYGLGDPELYKASVLHVE
                     KDSLYRRNKLLRQLVDIQYERNEFDIKPGIFRVRGDTLEILPAYSEKEIIRINFFGDE
                     IEAISRLHAVTGELIERVDAIDIYPAKHYVTEEKRMEKSLQDIEDELEERLKYFRDKG
                     MDLEAYRLEQRTRYDLEMLREVGYCSGIENYSRHFDRRAPGTAPWTLLDFLPSEYLLV
                     LDESHMMMPQIRGMYNGDRSRKEVLVEYGFRLPSALD"
     gene            1262..2341
                     /locus_tag="PK791_04230"
     CDS             1262..2341
                     /locus_tag="PK791_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOH92553.1"
                     /translation="MSEDKKVRVAIIGAGNCASSLVQGLYYYQNAKDDEQVPGLMHVN
                     LGGYHISDIEIVAAFDVVEGKVGKDLSEALFAYPNNTYKFCDIPFMNVPVHRGMTHDG
                     LGKYLSQIVHKAPGPTDDVVGILKETQADMLINYLPVGSEMATKWYMEQALRAGVGVI
                     NCVPVFIASSPYWSVRFREAGLPIIGDDIKSQVGATITHRVLTSLFVDRGVRLDHTYQ
                     LNFGGNTDFMNMLERDRLESKKISKTGAVTSMLPYKLDEDDIHVGPSDYVPWLKDRKY
                     CYIAMEGTTFGNVPLKAEVKLEVWDAPNSAGVVIDAIRCLKLAKDRGIGGPILAPSSY
                     FMKTPPEQYKDELARELTEAFICGE"
     gene            2573..>3755
                     /locus_tag="PK791_04235"
     CDS             2573..>3755
                     /locus_tag="PK791_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92554.1"
                     /translation="MDSSIITPQDPVQPSLTPEQEKKKKKAIWAWTMYDWGNSAFATT
                     IMAAILPVYFAQYIMLGESAVPIWGWAVAIGSLIAAVSSPVLGAIADFRGSKKTFMAF
                     FVALGVLSTAAMFFLTGPQHQTLTIVLYILGTVGFAGSLVFYDALLPHIADEDELDAV
                     SSKGYAMGYIGGGLLLAINVAMIFLGPKLMPNTPADEVTQLMMRLSMASVAIWWAVFS
                     IPVFKYVKEPPAKAYEHEIGEDVIKVGFGRFFSAFGNIRKFKDLAWFMLTFFVYSNGI
                     GTIITMATIIGTARGFSAITLIGTLLMVQFVAAPFSILFGKLAEKLGQKLTITISLII
                     YLLIATIGYFMSKEWHFYALGFAVAMVQGGSQAISRSLVGKLMPASKSAEFYGFFSVF
                     EK"
BASE COUNT          961 a         1007 c          854 g          933 t
ORIGIN      
        1 ttgtccagcg cggaaggcag acgaaaacca tattccacca aaacttcctt gcgcgaacgg
       61 tcgccgttgt acatcccgcg aatttggggc atcatcatat gcgattcatc taaaacaagc
      121 agatactccg atggtaaaaa gtccagcagt gtccagggcg cggtgcccgg cgcgcgccgg
      181 tcaaaatggc gagagtagtt ctcaatgccc gaacaatacc ccacctcgcg taacatctcc
      241 aggtcatagc gggtacgttg ttccaggcgg taagcttcca ggtccatgcc cttatccctg
      301 aagtatttca aacgttcttc aagctcatct tcgatatcct gcaaagattt ttccatgcgc
      361 ttttcttcgg tcacatagtg tttggcggga taaatatcaa tcgcgtcaac gcgctcaatc
      421 agttcgccag tcaccgcgtg cagacgcgag atagcttcaa tttcatcacc aaaaaaatta
      481 atgcggataa tctctttttc agaataagcc ggcagtattt caagcgtatc gccacgcacc
      541 ctaaaaatac cgggtttgat atcaaactca ttgcgctcat actgaatatc caccaactgt
      601 cgcaataact tgttccggcg gtacaggctg tccttttcca catgcaagac gctcgcctta
      661 tacagttccg ggtcgcccaa accataaatc gccgaaaccg acgctacgat aatcacatcc
      721 tgccgcgaca tcaacgccgt ggtggcagcc aggcgcaggc ggtcgatttc ttcgttaatc
      781 tgcgtttctt tctcgatata caggtctcgg cgcgggatat aagcttcagg ctggtaataa
      841 tcgtagtacg aaacgaaata ctccaccgcg ttgttcggga agaagtcctt atactccgcg
      901 taaagctgcg ctgccagggt tttgttatgc gtcatcacca gggttggcag ttgtaactgc
      961 tggataacgt tcgccacggt aaaagtcttg cccgtgcctg tcgcgccgag taaaacctga
     1021 tggcgatagc cctgacgcac accttttacc aagtccgcga tggcctgggg ttggtcgccg
     1081 gtgggtttgt atggagcctg aaggtcaaat tgcatggttc aattatagtc catgccctac
     1141 cggctatcct aatttgcatc atgtcctgat atgaacaaaa tgtaaagata aaagaaattg
     1201 atgtgaataa aacaaaggtt ttagagtatg attagaacca agttgaaaaa atggagaaat
     1261 aatgagcgaa gataaaaaag tacgtgtggc gattatcggc gcggggaact gcgcctcttc
     1321 actagtgcaa ggcctttatt actatcaaaa tgccaaagac gatgaacaag tgcccggctt
     1381 gatgcacgtc aatttgggtg gctatcacat cagcgacatt gaaatcgttg ccgcttttga
     1441 cgttgttgaa ggcaaggtcg gcaaagacct tagcgaagcg ctgtttgctt accccaacaa
     1501 cacctacaaa ttttgcgata tccctttcat gaacgtgccc gtgcatcggg gtatgaccca
     1561 cgatggactt ggcaaatacc taagccaaat cgtccataaa gcccccggcc caaccgacga
     1621 cgtggttggc attctcaaag aaacccaggc ggacatgctc atcaactacc tgcccgtcgg
     1681 ttccgaaatg gcgactaaat ggtacatgga acaagccctg cgcgccggcg tgggcgttat
     1741 caactgcgtg ccggttttca ttgccagttc gccctactgg tcagtgcgct ttcgcgaagc
     1801 tggcttaccc attatcggcg acgacatcaa aagccaggtt ggcgccacca ttacccaccg
     1861 tgttctaacc agtctttttg ttgaccgcgg tgtgcgcctt gaccacacct accaactcaa
     1921 ttttgggggc aataccgact ttatgaacat gctcgaacgt gaccgcctgg aaagcaagaa
     1981 aatcagcaaa accggtgctg ttaccagcat gctgccctac aaactcgatg aagacgacat
     2041 ccacgtaggc ccatccgatt acgtgccctg gcttaaagac cgcaagtact gttacatcgc
     2101 catggaaggt accacctttg gcaacgtgcc cctcaaggcg gaagttaaac tcgaagtctg
     2161 ggacgcgcct aactctgccg gcgtggttat tgacgccatc cgctgcctga aactcgccaa
     2221 agaccgtggc atcggaggtc ctatccttgc gcccagcagc tatttcatga aaaccccacc
     2281 cgaacaatac aaagacgaac tcgcccgcga acttaccgaa gctttcatct gtggcgagta
     2341 gaaaattagc gttaaataac cgaaataaat gtcaaaggtc cgttatagcg gacctttttg
     2401 attttcaaca gctggcagaa atggcaatta acatccctat actaaaggat taacttacac
     2461 ctgccagaca tatgcaataa tgattgaagt tataaactct ttacaatcaa atcaacttaa
     2521 atatatccat caagggtaaa attgatgcat ctcacaaaaa aaggagcgca ttatggattc
     2581 atccatcatc acccctcaag accctgttca gccatcttta acacctgaac aagaaaagaa
     2641 gaaaaagaaa gccatctggg cctggaccat gtacgactgg ggcaactcag cttttgctac
     2701 caccatcatg gctgccattc ttcctgttta tttcgctcaa tatattatgc tcggcgaatc
     2761 cgctgtaccc atttggggct gggcagtcgc catcggcagt ctgattgccg ctgtttccag
     2821 tccggtactg ggtgccatcg ccgactttag aggctcaaag aagaccttca tggccttttt
     2881 cgtcgccttg ggtgtactta gcactgccgc aatgttcttc ctaaccggcc cccaacatca
     2941 aactttgacc atcgtgctct acattttagg cacagttggt ttcgccggct cgcttgtctt
     3001 ctacgacgca ctcctgcccc acatcgctga cgaagacgaa ctcgacgccg tctccagcaa
     3061 gggctacgct atgggttaca tcggcggtgg attgttgctt gccatcaatg tggcaatgat
     3121 tttccttggc cccaagctga tgccaaacac ccccgcggat gaagtaaccc aactaatgat
     3181 gcgccttagc atggccagtg tcgctatttg gtgggctgtt ttctcaattc cggttttcaa
     3241 atatgtcaag gaaccccccg ccaaagccta cgaacacgaa atcggcgaag acgtgatcaa
     3301 ggtcggattt ggacgctttt tctctgcttt tggaaacatc cgcaagttca aagacctggc
     3361 ctggttcatg ctgaccttct tcgtctactc caacggtatc ggcaccatca tcaccatggc
     3421 caccatcatc ggcaccgcgc gtggctttag cgctatcacg ctgattggta ccctgttgat
     3481 ggtgcaattc gttgccgcgc cattctccat tctctttggc aaactggccg aaaaactggg
     3541 tcaaaaactc acgattacca tcagcctgat tatttacctg cttatcgcga caatcggcta
     3601 ttttatgagt aaggaatggc atttttacgc gctcggtttc gcggttgcca tggtacaagg
     3661 tggctcacag gccatcagcc gttcgttggt tggcaaactc atgccagcca gtaaaagtgc
     3721 tgaattttac ggattcttta gtgtctttga gaaat
//
LOCUS       DAOSFC010000132         3754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_22580_length_3754_cov_3.991890, whole genome shotgun sequence.
ACCESSION   DAOSFC010000132 DAOSFC010000000
VERSION     DAOSFC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22580_length_3754_cov_3.991890"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PK791_04240"
     CDS             <1..692
                     /locus_tag="PK791_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92555.1"
                     /translation="ACFVVAKNRFDIHLDALSETKQVLIVLDYSAASATELEPLASSA
                     LRAIEAQGASWQAVATRMDSLWLSASLYQKAGVEDPQAPVYLMGGQFAMLNLALEANS
                     ESQAASHSTLSDEKPRLDDFDYVLLVSDSSPTIRGWLEQVSPNQPELITLAIVSQKEA
                     VVIQPYFNSAQIAGFLTGAQGLSGENAVGGVNINVFRVGLMLMILLFVLGIVLSRETE
                     GIPPVTQEVDE"
     gene            689..1372
                     /locus_tag="PK791_04245"
     CDS             689..1372
                     /locus_tag="PK791_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92556.1"
                     /translation="MSGADLVLGIVGLVLTLMVFSYILKDNVFFGIALYLLVGVSSGY
                     ATLLIIRSVILPMLVSPLLKPGTQSFYLALVPLILSVLFVFMLFKRGSRMGRIPLAFL
                     VGVMVAMALFGVSRGTLAPQLLSVFQYFSPGVVAGHSTIRWWGLLEAIAIFLGVVAVL
                     AFFQERQRKMFGKTESSVVLTGMSKLGQVFLGITFGALFVGLLSSALIALIGNLTTIV
                     DFFRTLLGG"
     gene            1381..>2435
                     /locus_tag="PK791_04250"
     CDS             1381..>2435
                     /locus_tag="PK791_04250"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOH92557.1"
                     /translation="MSLFLSLNLSSNEIHSLLFERQAGLYALRAQNKMAFELDDAERL
                     MPAFRAQMQALEAETGIKMLDDDGHLAHEGHADLTGMSAVGFSFSASRPVRVGLVGIS
                     EAYSLSSLRRLVSLFDAEVVLETRLQDPLSQSQQLEALRAVDLDMLVIAGGSEEGASR
                     ALRNAIENARLFYHLMPKSIQPQIVYAGNQVLADYARLEIEAGDDFHLAPNLHQNTGE
                     EDLSVAWKAMLRAYQRVRLQQYPHLRHLQAELKTALLPASFAMGRLARFIARLSATDK
                     AVLLADLSDEKTSLLFANKNELMAISQANTIDEALIQSTQAYCSQNLTTAEVGEFLLN
                     QSLNPHQLSVTISDFAI"
     assembly_gap    2436..2535
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2536..2862
                     /locus_tag="PK791_04255"
     CDS             <2536..2862
                     /locus_tag="PK791_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92558.1"
                     /translation="VDNQNYEDHALLDLELVLPEDDSRQYFQAGRNQLCKIDTRGIES
                     MRVYLAPGRFSDVGMGMVGLGGWISVPDTELGLIIDARGRPLILPENPEARAKSWRNW
                     LWDLGG"
     gene            2864..>3754
                     /locus_tag="PK791_04260"
     CDS             2864..>3754
                     /locus_tag="PK791_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92559.1"
                     /translation="MKRVIIGENQTINRPVCLPTHGNLKVRVGEMVEFGNLIAESSQP
                     ERFEVIDIPSHFNLSPEQAEGTIKRLLGDVIAKGDVLAQKDGFITQLFRSPGDGKVVS
                     LRDGRITLAIGLEKEQVFAPMPGVVVELIGIRGAMISLTGTVVEGAWGNGKVAQGELV
                     FWEDLETNLLGVPGEMVKDKLIAFKKFATAGQIRRVLRLKPAGLILPSVSYKQFNLVS
                     ELDIVVISLLGFGETNMDTASMAMLETMDGKTVYLLAEESTWGPKLSPLIILPADKPA
                     DLGLTRQTARLEAGAKVRLMG"
BASE COUNT          806 a          845 c         1060 g          943 t
ORIGIN      
        1 ccgcgtgttt tgtcgtggcg aaaaaccgct ttgatataca tttagatgct ctctcggaaa
       61 cgaaacaggt tctcatcgtt ttggattatt ccgcggcaag cgcgacggag cttgagccac
      121 tggcaagttc ggctcttcga gccatcgaag cgcaaggtgc cagctggcag gcggttgcca
      181 cgcgcatgga cagcctttgg ctgtcggcaa gtttgtacca aaaagcggga gtggaagacc
      241 cgcaggcgcc tgtttattta atgggtgggc agttcgccat gctgaatttg gcacttgagg
      301 ctaattcgga gtcacaggca gcatcgcatt cgacactatc cgatgaaaaa ccaaggctgg
      361 atgactttga ctacgttttg ctggttagcg actcatcccc gaccattcgg ggctggctcg
      421 aacaagttag tccgaaccag ccggaactga taacgctggc gattgtcagc caaaaagaag
      481 cggtggtcat tcaaccctat ttcaacagtg cccaaattgc tggttttctg actggcgcgc
      541 agggcttgag tggtgaaaac gcggttgggg gtgtgaacat caacgttttt cgggtgggac
      601 ttatgctgat gattttgctg ttcgtgctgg gcatcgttct gagtcgcgaa acggaaggca
      661 ttccacctgt cacgcaagag gtggatgaat gagtggcgcg gacctggtgc tgggcatcgt
      721 cggtttggta ctgacgctga tggtgtttag ctatatccta aaggacaacg tcttttttgg
      781 catcgcgttg tatcttttgg tcggggtcag ttcgggctat gcaaccctgt tgattattcg
      841 ttcagttatt ttgcccatgc tggtcagtcc gctgttgaag ccaggcacac aaagttttta
      901 tttagccctg gtgcccttga ttctttcggt tttattcgtg tttatgcttt tcaaacgggg
      961 cagccgcatg gggcgtattc ctctggcgtt tttggtgggt gtgatggtgg cgatggcgct
     1021 gtttggcgtc agccgtggca cactcgcgcc ccagcttttg agtgtttttc aatacttttc
     1081 accgggcgtt gtggctggcc attcgaccat tcgttggtgg ggcttgcttg aagccatcgc
     1141 cattttcctg ggtgtggtgg cggtactcgc tttctttcag gaacgccaaa gaaaaatgtt
     1201 tggcaagact gagagttcag ttgttttaac agggatgtcc aaacttgggc aagtcttttt
     1261 gggcatcacc tttggggcct tgttcgtcgg gcttttgagt tccgcattaa ttgctttgat
     1321 tggcaatttg acgacgattg tcgacttttt cagaacttta ttagggggtt aaggccagag
     1381 atgagtctgt ttcttagctt aaacctgagt tcaaatgaaa tccacagcct gttgtttgaa
     1441 agacaagccg ggctgtatgc cttgcgcgcc caaaacaaaa tggcgtttga actggatgac
     1501 gccgagcgct tgatgccggc ttttcgggcg cagatgcaag cgctggaagc ggaaaccgga
     1561 atcaagatgc ttgatgacga cgggcatttg gcacatgaag gccacgccga tttgacaggc
     1621 atgagcgcgg ttggctttag cttttctgcc agccggccgg tgcgcgtagg cttggtgggt
     1681 atttctgaag cctattcctt gtcttcccta aggcgcttgg tgagcctttt tgacgcggaa
     1741 gtcgtgcttg aaacgcgttt gcaagaccca ctgagccaaa gtcagcaact ggaagccttg
     1801 cgcgcggttg acctggatat gctggttatc gcgggtggat ccgaagaagg cgccagtcgc
     1861 gctttgcgca acgccatcga aaacgcgcgc ttgttttatc atctcatgcc aaaatccatt
     1921 cagccgcaga ttgtttacgc cggcaatcag gttttggcgg attacgcgcg ccttgaaatt
     1981 gaagcgggcg acgatttcca tctcgcgcca aacttgcacc aaaacaccgg cgaagaagac
     2041 ctgagcgtag cctggaaagc tatgttacgt gcctatcagc gcgtgcgctt gcaacagtac
     2101 ccgcatttgc gccatcttca ggcggaacta aaaaccgccc ttttgccggc aagcttcgcg
     2161 atgggacgcc tggcgcgttt tattgcgcgc ctgagcgcta cggacaaagc ggttttactt
     2221 gcggatttgt ctgacgagaa aaccagtctt ttgttcgcta ataaaaatga actgatggca
     2281 atttctcaag ccaacacaat tgatgaggcg cttattcaaa gcacgcaagc ctattgttcg
     2341 caaaatctga ccacggcgga ggtgggcgag ttcttactca accaaagtct gaatccccat
     2401 cagctaagtg tgaccatttc cgattttgcc attgannnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnngttga caatcaaaac tacgaagacc acgcgctgct tgatttggaa
     2581 ctggtcttgc cggaagatga cagccgtcag tatttccagg cagggcgcaa ccaactctgc
     2641 aagattgaca cgcgcggcat tgagtccatg cgcgtttatt tggcgcctgg gcgttttagc
     2701 gatgtgggca tgggtatggt cggattaggt ggctggatat cggtgccaga cacggaactg
     2761 ggtttgatta ttgacgcgcg cggacgaccg cttattttgc ccgaaaaccc tgaggcgcgc
     2821 gcgaaaagct ggcgaaactg gctgtgggat ttgggtggct gagatgaaac gagtcattat
     2881 cggagaaaac caaactatta accgcccggt ttgcctgcca acgcatggca atttgaaagt
     2941 cagggtgggc gagatggtcg aatttggcaa tttgatcgct gaaagcagtc aacccgaacg
     3001 ttttgaggtg attgatatcc ccagtcattt caatctcagc ccagagcaag ctgaaggcac
     3061 catcaagcgt ttacttggcg acgtgattgc caaaggcgat gtgctggcac aaaaggacgg
     3121 ctttatcacc cagttgttca ggtcgccggg tgatggcaag gtggtgtcct tgcgcgatgg
     3181 acgcatcacg ctggcaatcg gtctggaaaa agaacaggtc ttcgcgccga tgcccggtgt
     3241 tgtcgtggaa ctgatcggca tacggggcgc gatgatatcg ctgaccggta cggtggttga
     3301 aggcgcctgg ggaaatggca aagttgctca gggtgagctg gttttttggg aagacctgga
     3361 aacgaacttg ttgggtgtcc ctggcgaaat ggtcaaagat aaactcatcg cgttcaagaa
     3421 atttgctact gccggacaaa tcagacgcgt tttgcgcttg aaaccagccg gcttgatatt
     3481 accgtccgtg tcgtacaagc aatttaacct tgtgagtgaa ctggatattg tcgttatcag
     3541 tttgctgggc tttggcgaaa caaatatgga cacagcgagt atggcgatgc tggaaacgat
     3601 ggacggaaaa acggtgtacc ttttggccga agaaagtacc tggggaccta aactgtcgcc
     3661 tttgatcatt ttgccggcgg ataagccagc cgatttgggt ttgaccagac aaaccgcgag
     3721 gcttgaagct ggcgcgaaag tgcgcctgat gggc
//
LOCUS       DAOSFC010000133         3711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_22929_length_3711_cov_4.474836, whole genome shotgun sequence.
ACCESSION   DAOSFC010000133 DAOSFC010000000
VERSION     DAOSFC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22929_length_3711_cov_4.474836"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PK791_04265"
     CDS             <1..336
                     /locus_tag="PK791_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOH92560.1"
                     /translation="TEADGKVIFLHKIVPGSADRSYGIHVAQLAGLPAPVITRANQVL
                     KQLEESGERALPKQIAETQQQIRLFPENNPMIDAFKAIDLESLSPIQALNLLYEWRLK
                     YFSDDSSDN"
     gene            complement(343..1263)
                     /gene="fmt"
                     /locus_tag="PK791_04270"
     CDS             complement(343..1263)
                     /gene="fmt"
                     /locus_tag="PK791_04270"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOH92561.1"
                     /translation="MSPRIVFLGSPDFAVPSLEELAGHYNVVGVITQPDRPFGRGQKM
                     APSAVKDAAQSLGLPVYQPESLRTLEAFSKLNAWQPDVMVVAAYGQILSQAVLDVPTK
                     GCLNVHASLLPRWRGASPIQAAIEAGDEKTGVTIMEMVQKLDAGPIVAQREVPLRSAT
                     YADELSQQLAFLGARTLLEVLPDWLNGTLKAVPQDESQVTYAPMLKKNQGLLSFDQPA
                     VQLVRKVHAYHPWPGTSFEYNGQRIKVLDAHVHDTFNAEPGIRYSVNGLPAIGTSECL
                     LVLDMVQPAGKRPMRGEDFLRGQRDWIERE"
     gene            complement(1316..1579)
                     /locus_tag="PK791_04275"
     CDS             complement(1316..1579)
                     /locus_tag="PK791_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92562.1"
                     /translation="MKDLFSSEKRANEIQEMEDFLRGKLGDLRPKIAFQASLKDKLIS
                     SETFQQKKRMGKAWLIGLGVALAGATFYGMGYLIYKNVSTRTI"
     gene            complement(1616..2182)
                     /locus_tag="PK791_04280"
     CDS             complement(1616..2182)
                     /locus_tag="PK791_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH92563.1"
                     /translation="MQDEIDPINEIELIDKAKKDPEAFGQLYDRYVVRIYNYIYYRVS
                     NTDDAEDLTAKVFFKALDNIGKYRHMGLPFSAWLYRIAHNQIANFHRDRSRVKEISID
                     DLVLSDAGTKPSPERAAQMNEEQKALLNLMNTLSPQKRDLIVLKFVHRLSNAEIGKIF
                     GKSEGAIKSLYHRTLLELRDLVERYDLS"
     gene            2502..>3711
                     /locus_tag="PK791_04285"
     CDS             2502..>3711
                     /locus_tag="PK791_04285"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOH92564.1"
                     /translation="MTDFLFKKDVADYDQHLADLVEFENERQARRLIMIPSESMAPLA
                     VRKLLGSSFQNIYAEGYPRPETRYQDEETIMDYGYQLGRYRRHSDPRYYKGVEYIDML
                     EALARRRAAELFAANGHVADDLWVNVQPLSGAPANTAVFTALLKPGDAILGMDLLHGG
                     HLTHGAPVNRSGLVYNAYHYTVDPKTERLDYDAILKIAQEVKPKILIAGYTSYPWIPD
                     WQKFREIADEVGAYFLADASHIAGLVAAGVVPSPVGIADIVSFTTHKTLCGPRGAVLI
                     TQDRELGEEIDKGVFPGEQGGPHMNSIAALALALKLATKPEFKELQQQTLKNTQALIK
                     GFKENGARVAYGGSDSHMALIDCKNFTGPDGTPLTGDLAARILDLVGIVTNRNTVPGD
                     RSALTASGIRM"
BASE COUNT          958 a         1015 c          819 g          919 t
ORIGIN      
        1 accgaagccg atggcaaggt cattttcctg cacaagattg ttcccggcag cgcggaccgg
       61 tcctacggca ttcacgtggc gcaactggct ggcctgcccg cgccagtcat cacccgcgct
      121 aatcaggtct taaaacaact ggaagaaagt ggcgaacgcg ccctgcctaa acagatagcc
      181 gaaactcaac agcaaatccg gcttttccct gaaaacaacc ccatgatcga cgccttcaaa
      241 gccattgatc ttgaatcact atcacccatt caggctttaa atttgcttta cgaatggcgt
      301 ttaaaatatt tttctgacga cagttcggat aattaaacga ccttattccc gttcaatcca
      361 atcgcgctga ccgcgcaaaa agtcttcgcc gcgcatcggg cgttttcccg ctggctgaac
      421 catatccaaa accaaaagac attcgctggt acctatcgcc ggcaaaccat tcacgctgta
      481 acggattccc ggttccgcgt tgaacgtatc atgcacgtgc gcgtcgagca ccttgatgcg
      541 ctgcccgtta tactcgaaag atgtccctgg ccaggggtgg taagcatgca ccttgcgcac
      601 aagttgaaca gcaggttggt caaagctgag aaggccctgg ttctttttaa gcatgggcgc
      661 gtaagttacc tgactttcat cctgaggtac ggcttttaga gtgccattca accagtctgg
      721 caaaacttcc agcaaagtac gcgcgcccaa aaaagccagt tgctgactga gctcatcagc
      781 ataagtcgcg ctgcgcaaag gaacctcgcg ctgggcgaca atagggcccg catcgagttt
      841 ttgcaccatt tccatgatgg tcacgccggt tttttcatca ccggcttcaa tcgcggcctg
      901 tatcggcgat gcaccccgcc agcggggcaa caaagacgca tgcacgttca aacaaccttt
      961 tgtaggaaca tcgagtacgg cttgtgagag aatctgccca taagccgcca cgaccatcac
     1021 atcaggttgc caggcgttga gttttgaaaa tgcttcaagc gtgcgtaagg attcgggctg
     1081 ataaacgggc aaacccaggg actgcgccgc gtctttcacc gcgctgggtg ccatcttttg
     1141 tccccgcccg aaaggacggt cgggttgggt aatgaccccc accacgttgt agtgcccggc
     1201 cagttcctca agactgggaa ctgcgaaatc tggagaacct aaaaagacta ttcttggtga
     1261 catatcaaaa ttttatcatg cttttttcgc gcaatgatgc gtggacagta agaccttaaa
     1321 tggttcgggt tgaaacgttt ttatagatga gataacccat gccataaaac gtggcacccg
     1381 ccagagcaac tcccaaaccg ataagccagg cttttcccat ccgttttttc tgctgaaaag
     1441 tttcggacga gatgagcttg tctttcaatg atgcttgaaa ggctattttg gggcgcaaat
     1501 cgcctaattt tccacgcaga aaatcttcca tttcctggat ctcatttgca cgtttttcag
     1561 aagaaaataa atctttcata ctcatactaa caaacacctt tcaagctttt tcgtttcacg
     1621 aaaggtcata cctttccact aagtcgcgta attcaagcaa agtccgatga tacaaacttt
     1681 taatcgcgcc ttcggacttg ccaaatatct ttccgatttc cgcgttactc aagcgatgca
     1741 cgaatttaag tacaatcaaa tcccgttttt gcgggcttaa tgtattcatc aaattgagta
     1801 aggccttttg ttcttcgttc atttgggctg cacgttcggg tgatggtttg gttccagcat
     1861 cagacagaac caaatcatca atcgagattt ctttcacccg cgaacggtcc cgatgaaaat
     1921 tagcaatctg attatgcgcg atacgataca gccaggcaga aaaaggcaag cccatgtgtc
     1981 tgtatttccc gatattatcc aaagccttaa agaacacctt tgcggtcaaa tcttcagcat
     2041 cgtctgtgtt gctgacgcga taataaatat agttataaat acgcacaaca tagcgatcgt
     2101 aaagttgccc aaaagcttcg gggtcttttt tggctttatc aatgagttct atctcattga
     2161 tggggtcaat ttcatcttgc ataacacttt attatacggg acttgggcga tattaccaga
     2221 attttgctta tatttcttta ctggcatcgc caaacacgaa caaataaaaa caaccgctct
     2281 ctcctattta actttcccat gagattaatt gcagttcagt gtttttcgcc agctttaagg
     2341 tacaatagaa tagatggaag tgtacattca cccaagctac aaaaataatc aaaattttag
     2401 atcttgtacc acacaagata atacttatgt caaatcccat ttgtccctgg cgggcaaatg
     2461 gttttgtttt accaaataaa atatcacaag gatgtaaata aatgaccgat tttcttttca
     2521 aaaaagacgt agcagattat gaccagcatc tcgccgattt ggttgagttt gagaacgaac
     2581 gtcaggctcg ccgactcatc atgatcccca gcgaaagcat ggcaccactc gctgtgcgca
     2641 agttactggg ctcatctttc caaaacattt atgctgaagg ttatccccga cctgaaacac
     2701 gttatcagga cgaagaaacc atcatggact acggctacca actgggcaga tatcgtcgtc
     2761 acagcgaccc ccgctattac aaaggcgttg agtacattga tatgctggaa gcactcgccc
     2821 gccgtcgcgc cgcggaactt ttcgcagcca acggacacgt cgctgatgac ctgtgggtca
     2881 atgtgcagcc actttccggc gcgcccgcca acaccgccgt ttttaccgcc ttgctcaaac
     2941 ccggcgacgc cattttgggt atggaccttc tccacggtgg ccatcttacc catggcgccc
     3001 ccgtgaaccg ctctggtctc gtctacaatg cctatcatta cacagttgac cctaaaactg
     3061 aacgactcga ctacgacgct attttgaaaa tcgcccagga agtgaaacca aagattctca
     3121 tcgcgggcta cacctcctat ccctggattc ccgactggca aaagttccgc gaaatcgccg
     3181 acgaagtcgg cgcgtacttc ctggccgacg catcccacat cgctggcctg gttgccgctg
     3241 gggttgttcc ctcgcccgta ggtatcgcgg atatcgtgag cttcaccacc cacaaaaccc
     3301 tttgtggccc gcgtggcgcg gtgctcatta cccaggaccg cgaacttggc gaagaaatcg
     3361 acaagggtgt cttccccggt gaacaaggtg gcccccacat gaacagcatc gccgcccttg
     3421 ctctcgccct gaaactggcg actaaaccag agttcaaaga actccagcaa caaactctta
     3481 agaacacaca agccttaatt aagggcttta aagaaaatgg cgcgcgcgtt gcctatggtg
     3541 gcagcgacag ccacatggca ctcatcgact gtaaaaactt tactggtccc gatggcacac
     3601 ccctcaccgg tgatctcgcc gcgcgtatcc ttgacctggt tggcatcgtt accaaccgca
     3661 acaccgttcc cggcgaccgc tccgcgctga ccgcatcggg tatccgcatg g
//
LOCUS       DAOSFC010000134         3700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_23027_length_3700_cov_4.593416, whole genome shotgun sequence.
ACCESSION   DAOSFC010000134 DAOSFC010000000
VERSION     DAOSFC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23027_length_3700_cov_4.593416"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PK791_04290"
     CDS             complement(<1..663)
                     /locus_tag="PK791_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HOH92565.1"
                     /translation="MKAKSMLSRSIFDLASKQSQLDLLRKEAESPTLWDDPISAQSLM
                     KKIASLERDVSTWVSINDKAVGLKELIEIDDGSFSDEISRQLDQLQKELDQKEIDLLL
                     SGEYDEGNALLTIHAGAGGTDSQDWAEMLERMYLRWADSQGFKTEILDLSPGEECGIK
                     SVTVSVEGQFAYGYLSQEIGVHRLVRLSPFDSAHRRHTSFALVEVLPEIEDNAEIIIR
                     PED"
     gene            complement(694..1620)
                     /gene="ftsY"
                     /locus_tag="PK791_04295"
     CDS             complement(694..1620)
                     /gene="ftsY"
                     /locus_tag="PK791_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOH92566.1"
                     /translation="MENIFNKWRESLKKTRDVTFGKIATYFGATEINDESWDRLEELL
                     IQADVGVKTTLDLIFVAQQIVDREGLTKTEQLKSVLKDEMSKLIVQPEEPDLDTYKPT
                     VIMVAGVNGSGKTTSIGKLAARYKKMGKSVLLVAADTYRAAAIDQLVVWAKRVDVPII
                     TSQQDSDPGAVTYDGIKAAIARNIDVVLIDTAGRLHTRFNLMEEIKKVHRVAGKALEG
                     APHYSWIVLDSTTGQNGLHQAEAFSKAVQLNGAILAKLDTSAKGGMVFGIREQIGLPV
                     IYAGLGEKVEDLELFDRANFIEGIFADVNGSK"
     gene            complement(1624..2676)
                     /gene="rlmN"
                     /locus_tag="PK791_04300"
     CDS             complement(1624..2676)
                     /gene="rlmN"
                     /locus_tag="PK791_04300"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOH92567.1"
                     /translation="MSKAFIYDLTPLELTETLNALEQPGFRTKQLLQGLYNKLYQDFD
                     EFDAIPKSLRETLDNQFEITPLKLHSTVYSKNKDTEKVLFETHDGHLIETVLMRFERR
                     NTLCISTQVGCPMACVFCATGQQGFARNLSMAEILGQFIHFQRELRQKNEALRNVVFM
                     GMGEPFLNYENVSNSLYALSDPERLGVGARHITVSTVGVIPRIAQFAKDHPQVNLAIS
                     LHAPDDALRTKLLPANKQFPLDKLMKACRDYVKETNRKITFEYALIDQLNDTTEHAHA
                     FAKQLKGILSIVNLITLNPSKEYPYPGSSTMRVQNFRNVLVSHGVRVTVRLRRGIEIN
                     AGCGQLAGSIKSNDII"
     gene            complement(2669..3466)
                     /locus_tag="PK791_04305"
     CDS             complement(2669..3466)
                     /locus_tag="PK791_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOH92568.1"
                     /translation="MLTDRFPQLKGLILDMDGVLWRDNEAIGNLPQIFDTIRKLGLSF
                     VLATNNSTKTIDSYLDKLNGFGVKLEPWQIIGTAQVTLEYLKQHFSAQSKIYVLGSIE
                     MKKLFSSNGFHILELDECPKADAVVVGLDKTVDYEKLRYASHLIRKGARFIATNTDGT
                     FPVPGGFIPGAGTIVKAVEVASVTKPVIIGKPEPFLFERAMQVMGTKPHETMAVGDRL
                     ETDIQGAHNAGILNALVLSGVATIEDSKTLSFEIDLIADNLWELLHV"
     gene            complement(3475..>3700)
                     /locus_tag="PK791_04310"
     CDS             complement(3475..>3700)
                     /locus_tag="PK791_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002869062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOH92569.1"
                     /translation="LHQPLGGTEGQASDIEIMAKEILRLKSSLNDFFAEVTGQPLEVI
                     QRDTDRDFYMTAQQALEYGLIDKVLEPPKK"
BASE COUNT          995 a          813 c          814 g         1078 t
ORIGIN      
        1 atcttcaggc cggatgatga tttctgcgtt atcttcaatt tcaggtaaaa cttcaaccaa
       61 agcaaaagat gtgtgccggc ggtgtgcgga gtcgaaaggt gataggcgta ccaagcggtg
      121 tacacctatc tcctggctca agtaaccata ggcgaattgt ccttcaacag aaacggtgac
      181 agatttaatg ccacactctt caccaggact caagtccagt atttcagttt taaagccctg
      241 actgtctgcc caacgcaaat acattcgttc aagcatttca gcccagtctt gtgaatccgt
      301 acccccagca ccagcatgaa tggttagcag ggcgttccct tcatcatact cgccagatag
      361 aagaagatcg atttcctttt gatcaagctc tttttgtagc tggtctagtt gacgactaat
      421 ttcatcgcta aagctgccat cgtcaatctc gattaattcc ttaagaccaa ctgccttatc
      481 gttgattgat acccaagtgc taacatcgcg ttcaagcgat gctatttttt tcattagcga
      541 ttgcgcggag atggggtcat cccacagtgt gggggattca gcttcttttc taagtaaatc
      601 taattgagat tgcttgcttg caaggtcaaa gatgctcctg gagagcatag atttggcttt
      661 caagtttttc cagcatttca atcatgtcag acattattta gacccattaa catctgcgaa
      721 gatgccttca ataaagtttg cgcggtcgaa aagttccagg tcttcaacct tttcgccaag
      781 tccagcgtag atgacaggca aaccgatttg ctctctaatc ccaaaaacca tgccaccttt
      841 ggcagaagtg tctaacttgg ccagaatagc accattgagc tgcacggctt tactaaaagc
      901 ttcagcttga tgtaaaccgt tttgaccggt ggtactatcc aaaacaatcc aggaataatg
      961 cggcgcgcct tcaagcgctt taccagcaac acgatggact ttcttgattt cttccatcaa
     1021 attaaagcga gtgtgcagac gacctgctgt gtcaatcagt acaacatcaa tgttacgtgc
     1081 aatggcagct ttgataccat cataggtgac tgcaccgggg tcactgtctt gttgactggt
     1141 gatgataggc acatccacgc gtttggccca tactacaagc tgatcaattg ctgcggcacg
     1201 ataagtatcc gctgctacca aaagaactga tttacccatc tttttatagc gcgctgcaag
     1261 tttgccgata gacgtcgttt ttccggaacc gtttacgcca gctaccatga tgaccgttgg
     1321 tttatatgtg tctaaatcgg gttcttcagg ttggacaatg agcttgctca tttcatcttt
     1381 caaaacagac ttgagttgtt ccgtcttggt cagaccttct ctgtcaacaa tttgttgggc
     1441 gacgaaaatc aaatcaagtg ttgttttaac accaacatcg gcttgaatga ggagttcttc
     1501 aagccgatcc cagctttcat cattaatttc agttgcacca aaataagttg cgattttacc
     1561 gaaggtaaca tcacgggttt tcttaagact ttctcgccat ttgttaaata tgttttccat
     1621 agattagatt atatcatttg acttgatgga accggctaat tgtccacagc cggcgttgat
     1681 ttcaattcca cgccttaatc gcacggttac ccggacacca tggctaacta atacatttcg
     1741 gaagttttgt actcgcatag tagatgaacc agggtatgga tattccttac ttggattaag
     1801 tgtgattaag ttgacaatgc tcaagatgcc tttgagttgt ttcgcgaacg catgggcatg
     1861 ctctgttgtg tcattaagtt ggtctataag cgcgtattca aatgtgattt ttcggtttgt
     1921 ttcttttaca tagtcgcggc aggctttcat cagcttatct agtggaaatt gcttgtttgc
     1981 gggtaacaat ttagttcgca aggcatcatc tggcgcgtgg agtgaaatcg caaggttgac
     2041 ttgtgggtga tctttagcaa attgagcaat tcgtgggata acaccgacag tgctgacggt
     2101 tatatgacgc gcgccaacac ctaagcgttc aggatccgat aaagcataaa gagaattaga
     2161 gacgttttcg tagtttaaaa aaggttctcc catacccata aacacgacat tgcgtaaagc
     2221 ttcatttttt tgacgaagtt cacgttgaaa gtgaatgaat tgtccaagaa tctccgccat
     2281 acttagatta cgagcaaaac cttgttgacc ggttgcgcag aagacgcaag ccatagggca
     2341 accaacctga gtagagatac aaagagtatt gcgacgttca aaacgcatca ggactgtttc
     2401 gataagatgc ccatcgtgtg tctcaaacaa aaccttttca gtatctttgt ttttcgaata
     2461 gactgtgcta tgcaacttta acggagtaat ctcaaattga ttgtctaagg tctcacgtaa
     2521 agatttcggg atagcatcaa actcgtcaaa atcctggtag agcttattgt agagcccctg
     2581 taaaagttgt tttgtgcgga aaccaggttg ttcaagcgca ttcaaggttt ctgtaagttc
     2641 taagggggta aggtcgtaaa tgaaagcttt agacatgcag gagctcccat aaattgtctg
     2701 cgattaggtc aatttcaaac gaaagtgttt tactgtcttc gattgtagcg acccctgaca
     2761 aaaccagagc atttaagata cctgcattat gcgcgccttg aatatctgtt tcaagacgat
     2821 cgcctacagc catggtttca tgtggctttg tacccatgac ttgcatcgct ctctcgaata
     2881 agaagggttc tggtttgccg ataataacag gctttgttac actggcgact tcaaccgctt
     2941 tgacaattgt gccggcacct gggataaaac cgcctggaac aggaaaggta ccatcagtgt
     3001 tggtggcgat aaagcgcgcg cctttacgga tcaaatgact ggcatagcgt aatttttcgt
     3061 aatcaacggt cttatctaaa cccacaacaa cggcgtctgc tttaggacat tcatcaagct
     3121 caaggatatg aaaaccgtta gacgagaata gctttttcat ttctatactg cctagcacgt
     3181 atatcttact ttgagcagaa aaatgctgct ttaggtattc gagcgtgacc tgtgctgttc
     3241 cgatgatttg ccagggttca agttttacgc caaatccatt gagtttatcg agatacgaat
     3301 caattgtttt ggtggaattg ttggtcgcca aaacgaaaga aagccccagc tttctaatgg
     3361 tatcgaaaat ctggggcaaa ttgccaatag cttcattgtc gcgccaaagg acaccatcca
     3421 tgtcgagaat aagccctttg agttgcggga atcgatccgt aagcatgaaa aatgttactt
     3481 cttaggtggt tccagcacct tgtcgattaa gccatattcc aaagcttgtt gggcggtcat
     3541 gtaaaagtca cggtctgtat cacgctggat aacttccagg ggttgaccgg tgacttcagc
     3601 aaagaaatca ttgagagaag acttcagtcg caggatttct ttggccatga tttcaatatc
     3661 cgatgcctga ccttccgtgc cacccaaagg ctgatggagg
//
LOCUS       DAOSFC010000135         3647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_23492_length_3647_cov_4.292316, whole genome shotgun sequence.
ACCESSION   DAOSFC010000135 DAOSFC010000000
VERSION     DAOSFC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23492_length_3647_cov_4.292316"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PK791_04315"
     CDS             <1..487
                     /locus_tag="PK791_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92570.1"
                     /translation="DPAKAQAKWNSALKAVLDPASYLPIRIVSYTIGALFLACIKKHT
                     DFDFESFDDQPFSLKILEGVQPATLPVQPSPAIEAILKAYQAETNAIIENTLAKNERV
                     LEGKFPLIMLNVYNARWNGRYATSSYFIAYQDDGEMKTLYGDFIVEIDKDYNILTAYR
                     Q"
     gene            578..928
                     /locus_tag="PK791_04320"
     CDS             578..928
                     /locus_tag="PK791_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH92571.1"
                     /translation="MIKRKDTLTTIPSPALLGGEGTIYRTAFLQPEEFHGKGRLFAHM
                     RIPPGSSIGVHVHEGEQETFFILSGNGEVLEKGEWLPIGPGDVAICPNGCQHGIRNTG
                     NEDIEMIALILNMD"
     gene            983..1591
                     /locus_tag="PK791_04325"
     CDS             983..1591
                     /locus_tag="PK791_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOH92572.1"
                     /translation="MKDFSKLIKTLEHLGYTVREFSTAKEASLYLTEAIQNYHVGFGG
                     SKTLEAMGLFDLLSEKNAVHWHWFPRNNLSRAETLKKVRDAEIYISSVNGIAESGEII
                     NIDGTGNRVAAISHGHQKVYLVIGENKIAPTFEDALYRARNVAAPLNAQRLGVDTPCA
                     IKGDKCYDCDRPHRICRNLSVLWRKPTGAEYEIILIHENLGY"
     gene            1771..>3647
                     /locus_tag="PK791_04330"
     CDS             1771..>3647
                     /locus_tag="PK791_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOH92573.1"
                     /translation="MTDKEIRPFGTWPSQISAEMLSVGIRLNDVQWARGSDTLVWSQS
                     FEGQTTLFAKPKDALPYPLTDENNKPSGGIAYGGGEFFAAEDGVIFTDRNGRLYRKSY
                     QDGAPQAITPGFGSAATPVLSHNKDFVIYAHSYDGIDRLAATRADGKTWPVILQEGAD
                     FYLNPCLSPDDRHLAWIEWNHPNMPWDGTSLKIAKLDPQSLSLSEIQTLDGFGEYACY
                     QASFSPCGNFLAWLANTGEFDDLIVYDLRTKSKLTFISQKSLLQPAWIAGQHTLAWAP
                     DSLAIYYIDNDLGTLSLKKLNLLKRHSEENAITTVDIGDFTWLDQLSVSARGEIAFIA
                     QSSAEDSQIVRLHPDGTEIIFKSMAEALPVGYFAIASPFSWQSSDGVEVHGFYYPPTN
                     PDYTAEGLPPVITIIHGGPTSANYNDFDMSTAFFTSRGYAVFKLNYRGSTGFGKSYRN
                     ALQNNWGKIDLQDAIEGTQALIDAGLADPSNLVIMGGSAGGFTVLNALIHHPGFFKLG
                     ISRYGVSNLFMLEEDTHKFESHYNTSLLGALPEHHDEWQARSPVFHADNIRDALLLFQ
                     GSADPVVPQAQSDSLVEQLKANNVPHLYRVYEGEGHGFRQAENLIDYYNTIDACLKEM
                     V"
BASE COUNT          952 a          974 c          847 g          874 t
ORIGIN      
        1 cgaccctgcc aaagcgcaag ccaaatggaa tagcgcgtta aaagccgttt tggacccagc
       61 cagctacctg cccatccgca ttgtgtccta cacaatcggc gccctgtttc tcgcttgtat
      121 taaaaaacat acggattttg attttgaaag cttcgacgac cagcctttct cattgaaaat
      181 cctggaaggc gttcagccag ccaccttgcc tgtccagcct tctccagcta ttgaagccat
      241 cctgaaagcg tatcaagctg aaaccaacgc catcatcgaa aacacgttgg caaagaatga
      301 gcgtgtgctt gaaggcaaat tccccctgat catgcttaac gtttacaacg cgcgctggaa
      361 tgggcgttat gccacatcaa gctatttcat cgcttaccag gatgacgggg agatgaaaac
      421 gctatatggc gactttattg tggaaatcga caaggattac aatatcctga ccgcatatcg
      481 acaatagccc atattttctt agtttctgtt tccattctta acgctttacg cattctcaag
      541 cgcgaacttt gtggtttaat aaggcttagg agacattatg attaaacgaa aagacacctt
      601 aacaactatc cccagccctg cccttttggg cggtgaaggc accatctatc gcaccgcatt
      661 tttgcagccc gaagaatttc atggcaaagg tcgccttttc gcccacatgc gcatcccacc
      721 cggcagttct atcggcgtgc atgtccacga aggcgaacag gaaacctttt tcattctcag
      781 tggcaacggt gaagtgctgg aaaaaggcga gtggctgccc atcggtcccg gtgatgtcgc
      841 catctgcccc aacggttgcc agcatggcat ccgcaacaca ggtaatgaag atatcgagat
      901 gatagcgtta attctcaaca tggattaaca ctgcgaggct catgtttaac actggttcaa
      961 ctgaaaaagg agattgagta gcatgaaaga cttcagcaaa ctgataaaaa cccttgaaca
     1021 cctgggctat acggtgcgcg aattttcgac tgccaaagaa gccagccttt acctgacaga
     1081 agccattcaa aattaccatg ttggctttgg tggctccaaa acgctggaag cgatgggatt
     1141 gtttgacctg ctttctgaga aaaacgcggt ccactggcac tggtttccgc ggaataatct
     1201 aagcagggca gaaacattaa agaaggttcg cgacgcggaa atttacatca gttcggtgaa
     1261 cggaatcgct gaatctggcg aaattatcaa tatcgacggt actggcaacc gcgtggcggc
     1321 catcagtcac ggccatcaaa aagtttacct ggtgatcggc gaaaacaaaa ttgccccaac
     1381 ttttgaagac gcgctgtatc gcgcgcgcaa cgttgccgcc ccgctcaacg ctcagcgttt
     1441 gggtgtggac accccttgcg ccataaaagg tgataaatgc tatgattgtg atcgcccaca
     1501 ccgcatttgc cgtaatttat cggtgctatg gcgcaaacct accggcgcgg aatatgaaat
     1561 catattaata cacgaaaacc tgggttatta atttttcagc caaataggac aactatttgg
     1621 cagtaacaat ttttattgtg attcttttcc gtttgatctg tctcggcctc cagcaagaca
     1681 gatttgaact gtattaaagc tatctctaaa atccgagcgt tttgaggcaa agcctgatat
     1741 gattaagcca aagacaataa ggattcgcct atgacagaca aagaaattcg accttttggc
     1801 acctggccat cgcaaatcag cgctgagatg cttagtgtgg gcatccgcct gaatgacgtg
     1861 caatgggcgc gtggcagcga caccttggta tggtctcaat cgtttgaagg acaaacgacg
     1921 cttttcgcca agccaaaaga cgccttgccc tacccactga ccgacgaaaa caacaagccc
     1981 agcggtggca tcgcttacgg tggcggtgag ttttttgccg cggaagatgg cgtcatcttc
     2041 accgaccgca acggtcgcct ttaccgcaaa agctaccagg atggcgcacc ccaggccatc
     2101 accccaggct ttggcagcgc cgcgacgcct gttctctcac acaacaaaga cttcgttatc
     2161 tacgcgcata gctatgatgg cattgaccgc cttgccgcca cacgcgccga tggcaaaacc
     2221 tggcctgtca tcctgcagga aggcgcggac ttttatctca acccctgcct aagcccggat
     2281 gaccgccatc tcgcctggat tgagtggaac caccccaaca tgccctggga tggcacaagt
     2341 ctaaaaatcg ccaaactcga cccacaaagt cttagcctaa gcgagattca aacgctggac
     2401 ggtttcggtg aatacgcctg ctatcaggcc agtttttcac cctgtggaaa cttcctggcg
     2461 tggctggcaa ataccggcga gtttgacgac cttatcgtct acgacctgcg caccaaaagc
     2521 aagctgactt tcattagcca aaaaagtctc ctgcaaccgg cctggattgc tggccaacac
     2581 acgctcgcgt gggcgccaga cagccttgcc atttactata tcgataacga cctgggcact
     2641 ttaagcctta aaaagcttaa tcttctcaaa agacattcag aagaaaatgc cattaccaca
     2701 gttgatattg gcgattttac ctggctcgac cagcttagcg tgtctgccag gggcgaaatt
     2761 gccttcatcg cgcagtcttc tgctgaagac tcacagattg tgcgacttca tcctgatggc
     2821 acagagatta tttttaagag catggctgaa gccttgcccg ttgggtactt cgcgattgcc
     2881 agccctttta gctggcaaag cagcgatggc gtggaagtgc atggctttta ttatccaccc
     2941 accaaccctg attacaccgc ggaaggtttg ccaccggtca tcacaatcat tcacggtgga
     3001 cccacatccg caaactacaa tgattttgat atgagtaccg cctttttcac cagccgtggc
     3061 tatgccgtgt ttaagcttaa ctaccgtggc agtactggtt ttggaaagtc ctaccgaaac
     3121 gctttgcaaa ataactgggg caaaattgac cttcaggacg ctatcgaagg cacacaggca
     3181 ttgattgatg ccggtttggc tgaccccagc aatctggtca tcatgggtgg cagcgcgggt
     3241 gggtttaccg tgctgaacgc gctcatccac catcccggct ttttcaaact gggcatcagt
     3301 cgctatgggg tatctaatct ctttatgctg gaagaagata cccataaatt tgaatcccac
     3361 tacaacacca gtctgctggg tgccctgccc gaacatcacg atgaatggca ggcgcgctca
     3421 ccggtatttc atgccgacaa tattcgcgac gcgctgttac ttttccaggg ttcggcagac
     3481 cccgtcgtcc cccaggcgca aagcgatagt ctggttgaac aacttaaagc caacaatgtg
     3541 ccccatcttt accgcgttta cgaaggcgaa ggtcacggct ttagacaagc tgaaaaccta
     3601 atcgattatt acaatactat cgatgcctgt ctcaaagaga tggtcat
//
LOCUS       DAOSFC010000136         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24093_length_3584_cov_6.219326, whole genome shotgun sequence.
ACCESSION   DAOSFC010000136 DAOSFC010000000
VERSION     DAOSFC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24093_length_3584_cov_6.219326"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1142
                     /gene="holB"
                     /locus_tag="PK791_04335"
     CDS             138..1142
                     /gene="holB"
                     /locus_tag="PK791_04335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOH92574.1"
                     /translation="MTWKIFGHDGAVNYLKRQCEPGNMRHAYLITGPEGIGRLTLAKA
                     FVKALNCQNPPAKHDFCNACQACRQIEAEAWPDLTILRPTENARDIKIDQVRQMQQSL
                     ALAPYQSQWRVVIIPDFQNATVAASNALLKSLEEPPARAIIILTADARENLLETIASR
                     CGILRLRPMSVGAVARVLQSELNLDEDDAKMIAHLAGGRVGTAIHFAQNPDLFEQYHD
                     TLAQLHEILPETKRARLQYAEGVSKRKGSQRENISTLLSVWLTFWRDVMIAHSGADLP
                     IVNLVQRDVVKTVAGQLSLPQIESILKAHEKGLEQIDANVNSRLVLENILLNIPRLKV
                     "
     gene            1148..2401
                     /gene="chrA"
                     /locus_tag="PK791_04340"
     CDS             1148..2401
                     /gene="chrA"
                     /locus_tag="PK791_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HOH92575.1"
                     /translation="MTEKELTPESVTTPGDQTPEENALEKASEIPLRKRLLDLMLVNL
                     KIGSTSFGAPTAHVAMMEREYVHKHKWVTPQHFLDLLSAANIIPGPNASETCYHVGYV
                     HAGYPGLLVAGISFILPAIISALILAWVYMRYGALPEMVGLFYFLNPLVLAIVLDSTF
                     RLAKSSYTNWKQIPLFIAIIVAKILGVDEVVILLGAGLVMIVVHFIQNGFPKNKKTPP
                     SMMLFVLPVVELIEKYLPTVKNIFLYFLRTGTVLFGSSMVLFGLIQDDIVNRFGWLSM
                     QQLTDAIAIGQITPGPVLASSTYVGFLASGLNGTFLDGLKGGLAATAGVFLPSFVIVA
                     VTAPIVKKMRESELASAFLSGVNVATVALILMVSVTLAQNGVVDIWTALLALGGLAAL
                     IFLKVPPWVLVLSGLAMGALRIFVF"
     gene            2432..>3584
                     /locus_tag="PK791_04345"
     CDS             2432..>3584
                     /locus_tag="PK791_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOH92576.1"
                     /translation="MDDLLKILNEQQQKAVAAPAGPTIVLAGPGSGKTRVLTYRIAYL
                     IGAMNVPAWHILAVTFTNKAAREMGDRVNKILGSNAEGIWLGTFHSVCGKILRREADL
                     LPVTRDFVIFDQDDQTTLLKEIIKENNLDEKMYRPASYLNEISRAKNDLITPENYPIK
                     DFRDEQTQRLYRIYQERLIKNNAMDFDDMLVYTTQLLDEYPIVRETYSHKFEHILVDE
                     FQDTNMAQYMIIKNLASLHQNLYLVGDEDQSIYRWRGADYRNIERFKRDFKNAKVYLL
                     EQNYRSTQTILDSAMAVIEPNKNRTHKQLETVRSKGEKITMIVAKDDRDEADSVVRAI
                     REGIYTKRQNPSDFAIMYRTNAQSRLFEEAFRREGINYRLVGAQRFYGRR"
BASE COUNT          902 a          972 c          879 g          831 t
ORIGIN      
        1 atgttcctgt gtggtgggct ctatttgggc atccatcttc tgctgggaag tgtgtagtac
       61 accttataca cgaaagtttt aaacaagaac cccgctggct tacgctttta agcttataga
      121 tgtgctaaaa ttcccttatg acctggaaga tttttggcca cgacggcgcg gtgaattatc
      181 tcaaacgaca atgcgaacct ggcaatatgc gccatgccta tctcatcacg gggcccgaag
      241 gcatcgggcg attgacgctc gccaaagcct ttgtaaaagc gcttaactgc caaaacccgc
      301 ctgccaaaca cgacttttgc aacgcgtgcc aggcctgccg gcaaattgag gcggaagcct
      361 ggcccgattt gaccatcttg cgccccaccg aaaacgcgcg ggatatcaaa attgaccagg
      421 tgcgccagat gcaacaaagc ctggcgctgg caccctatca gagccaatgg cgcgtggtga
      481 ttatccccga ttttcaaaac gcgaccgtcg cggcatcgaa cgccttgctc aaatcgctcg
      541 aagaaccccc cgcccgggcg attatcatcc tgaccgccga cgcgcgcgaa aacctgctgg
      601 aaaccatcgc ttcgcgctgt ggcatattgc gtctacgccc gatgagcgtg ggcgctgttg
      661 ccagggtctt gcaaagcgaa ctgaatttgg atgaagatga tgcaaaaatg attgcccatc
      721 tcgccggcgg acgcgtggga acagcgatac atttcgcgca aaatccggac ttgttcgaac
      781 aataccatga tacgctcgcc cagctacacg aaatcttgcc ggaaacaaaa cgcgcgcgcc
      841 tgcaatacgc tgaaggcgtt tcaaagcgca agggcagtca gcgcgaaaat atctccaccc
      901 tgctttcggt ttggcttacc ttttggcgcg acgtgatgat tgcccacagc ggagcagact
      961 tgcccatcgt gaatctcgtg cagcgtgatg tagtcaaaac agttgccggc cagcttagcc
     1021 tgccccaaat tgaatcgata ctcaaggctc atgaaaaggg gctggaacaa atcgacgcga
     1081 acgtcaattc gcggcttgtg cttgaaaata tcctgctcaa tatccccaga ttgaaagttt
     1141 aaaatccatg actgaaaaag aacttacgcc cgaatctgtc accacgcctg gtgaccaaac
     1201 ccccgaggaa aatgcgctgg aaaaagccag cgaaatccca ttgcggaaac gcttgctgga
     1261 tttgatgctg gtcaatctca aaatcggctc caccagtttt ggcgcgccca ccgcgcatgt
     1321 ggcgatgatg gaacgcgaat acgtgcacaa acacaaatgg gttaccccgc agcacttttt
     1381 ggatttgctc agcgcggcaa acatcatccc cggccccaac gccagcgaga cctgctacca
     1441 tgttggttat gtgcacgcgg gctatccggg cttgttggtc gcgggcatca gtttcatctt
     1501 gccagcgatt atctcggcgt tgattttggc ctgggtttac atgcgctatg gcgcgttgcc
     1561 cgagatggtg gggctgtttt acttcctcaa cccactggtt ttagccatcg tgcttgattc
     1621 aacttttcgt cttgccaaaa gcagttacac caactggaag caaataccgc tgtttatcgc
     1681 gataatcgtc gcgaaaatac tgggagtgga tgaagttgtc atcctgcttg gcgcgggctt
     1741 ggtgatgata gtggttcact ttatccaaaa tggttttcca aagaataaaa aaacaccgcc
     1801 atcaatgatg ctcttcgttt tgcctgttgt ggagctcatc gaaaaatacc tgcccactgt
     1861 caaaaacatc ttcctttact ttttgcgcac gggcacggta ttgtttggct catcaatggt
     1921 gctgtttggt ttgatacaag acgatatcgt caatcgcttt ggctggctga gcatgcaaca
     1981 actcaccgat gccatcgcca tcgggcaaat cacacctgga ccggttttgg catcaagcac
     2041 ctatgttggt tttttggcct cgggtctgaa tggcactttt cttgatggat taaaaggagg
     2101 acttgccgcc acggctggag tctttttacc ttcgttcgtg attgtcgcgg tcaccgcgcc
     2161 tatcgtcaaa aaaatgcgcg agagcgaact tgccagcgcg tttctttccg gcgtgaatgt
     2221 cgccacggtc gcgctcatcc tcatggtcag cgtcaccctg gcacaaaacg gcgtggtgga
     2281 tatctggacc gcgttgctgg ctttaggcgg tctggcagct ttgatattcc tgaaagtccc
     2341 accatgggtg ctggtgcttt ctggtttggc aatgggcgcg ttaagaatat ttgtcttcta
     2401 agatggtatg ataaagtctt agggcaaaac catggacgat ttactgaaaa ttctcaacga
     2461 acaacaacaa aaagccgtcg cagcgccagc tggtccgacc atcgtgttgg cagggccggg
     2521 ctcgggcaaa acccgtgtgt taacctaccg catcgcctat ttaatcggtg cgatgaacgt
     2581 gcccgcttgg catattttgg cggtcacgtt taccaacaaa gccgcgcgcg agatgggcga
     2641 ccgcgtgaac aaaattctgg ggagtaacgc tgaaggcatt tggttgggca ccttccactc
     2701 ggtttgtggc aaaatcctgc gtcgcgaagc ggatcttctg cccgtcaccc gcgattttgt
     2761 catttttgac caggacgacc aaacaacact tctcaaagaa attatcaagg aaaacaacct
     2821 tgacgaaaag atgtatcggc ccgcatccta tcttaacgaa atttcgcgcg ccaaaaacga
     2881 cctgattacc cccgaaaact atcccattaa agactttcgc gacgaacaaa cgcaacggtt
     2941 gtatcgcatc tatcaggaac ggctcatcaa aaacaacgcc atggattttg acgacatgct
     3001 cgtttacacc acgcaactgc tcgacgagta tcccatcgtg cgcgaaactt actcgcacaa
     3061 gtttgagcat attctggtgg acgagttcca ggacaccaac atggcgcagt acatgattat
     3121 caaaaacctt gccagtctgc accaaaatct ttaccttgtc ggtgacgaag atcaaagcat
     3181 ctatcgctgg cgcggggcgg actatcgcaa cattgaacgc ttcaaaagag atttcaagaa
     3241 cgccaaagtc tatctgcttg aacaaaacta ccgctccacg caaaccattt tggacagcgc
     3301 catggcggtt atcgagccca acaaaaaccg cacccacaaa caactcgaaa ccgtacgatc
     3361 caaaggcgag aaaattacca tgatcgtcgc taaagacgac cgcgatgaag ccgattcagt
     3421 cgttcgggca atccgagaag gcatatacac caagcgccaa aatccgagtg atttcgccat
     3481 catgtaccgc accaacgccc aatcgcgcct ctttgaagaa gcttttcgac gcgaaggcat
     3541 caactaccgc ctggtcggcg cgcaacgctt ttatgggcgg cgcg
//
LOCUS       DAOSFC010000137         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24235_length_3569_cov_5.797097, whole genome shotgun sequence.
ACCESSION   DAOSFC010000137 DAOSFC010000000
VERSION     DAOSFC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24235_length_3569_cov_5.797097"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PK791_04350"
     CDS             <1..746
                     /locus_tag="PK791_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92577.1"
                     /translation="GWFERLDALEIALVKKGSWYFETESHQATNDPLNEPGYKISFTH
                     IHEDRIECDEYIEFEPSWGSITEIYDYAKAGEFNITFYRKPGYPEQYYVESPDACCSY
                     YSVKDSDWRRPFILTNFYPTFEHTLYLLKLNGDAFGKNEFKMWFEKTENAELLHFEEN
                     AWDTTGGFFTSTNSEILPITAKTTHFIFDVATGQMIYEFETLKSYNNESKTDEVYYYQ
                     LAMNDIPQIYKDAMNKLYLDLGKPPRWAD"
     gene            complement(777..2114)
                     /locus_tag="PK791_04355"
     CDS             complement(777..2114)
                     /locus_tag="PK791_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOH92578.1"
                     /translation="MHDNGKKTSWWKRFTDKLNREFRWFLPGLGYKRWLALTVIGAML
                     LGLGLAVIVLQYYRTTTNEFLTPILAVLSLRFLDRPIRFLLFGGIGFTLILFGIWGAN
                     RAVLKPFEREGRPAWEAVQQYRRKDRGIKVVAIGGGHGLATLLSGIKAYTHNITAIVT
                     VSDDGGSSGELRRDLGVLPPGDIRNCLSALSNDEALLSQVFQYRFKSGAGLEGHSLGN
                     LFITALAEITGSFEEAVAESGRVLAIYGKVLPSTLSNVQLVGDVRLHDSDEIVHIKGE
                     SKITGANGRILRVLLDPSNAQAYPPTVAAILNADLILIGPGSLYTSILPNLLVSGLVD
                     AIRSSRAEVFLVSNVATQRGETDGFNVSDHMHVLEQHIGKNIFDLVISNNNFKGDLGE
                     GVDWVRNDATSRRSYKVYETDLVDGLYPWRHDSKKLAKAIISVFEERTGPLFD"
     gene            complement(2193..3116)
                     /locus_tag="PK791_04360"
     CDS             complement(2193..3116)
                     /locus_tag="PK791_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOH92579.1"
                     /translation="MAIPDFQTIMLPLLKLASDGKEHYIHDAVEELSNYFKLTEEEKG
                     ALLPSGTQSVFYNRVGWARSYMKAAKLLEDPRRTYFKITQRGLDLLAENPKAINNKIL
                     SRYPEFVKFRQRRPQRSDEIVEKSETDSIETPEEIMESAYQELRETLAAEVLNVVTST
                     SPGFFEKLVVELLHKMGYGGTQRELARAVGRHGDEGIDGVIDQDKLGLDTIYIQAKNW
                     KSDSSIGRPEIQKFVGALFGQHARRGVFITTAKFTQDAINYAKKIDAKVILIDGERLA
                     ELMIDYNVGVTLNTSYEIKKIDYDFFSDEAL"
     gene            complement(3153..>3569)
                     /locus_tag="PK791_04365"
     CDS             complement(3153..>3569)
                     /locus_tag="PK791_04365"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92580.1"
                     /translation="HKQVGSSQGHPLARTSPLNELRVAGVDERLKVCKNAILARDFEA
                     FAWATELDSEWMHAVMRTSTPSLRYWTELTELILWQVRYWRQQGHALCTTVDAGPNVH
                     VIGTQNELDFALPALKALPGVMDVIVARPGAGAGLI"
BASE COUNT          992 a          890 c          729 g          958 t
ORIGIN      
        1 ttggctggtt tgaaagacta gacgcgcttg aaatagcctt agttaaaaaa ggaagctggt
       61 attttgaaac cgaatcacat caagctacaa acgatccgct taacgaacct ggttataaga
      121 tctcctttac gcatatacac gaagaccgca ttgaatgtga cgaatatatt gagtttgaac
      181 caagctgggg cagtattacg gaaatttacg attatgctaa ggcaggagaa tttaatatta
      241 ccttttatag aaaacctgga tacccagagc aatactacgt agaaagtcca gacgcctgtt
      301 gtagctatta cagtgttaaa gattcagact ggcgtcgccc attcatacta actaattttt
      361 accctacttt tgaacataca ctatatctat tgaaattaaa tggtgatgcg tttggaaaaa
      421 atgaatttaa aatgtggttt gaaaaaactg aaaacgctga attattacat tttgaagaga
      481 atgcatggga tacaacgggt ggctttttta ccagcactaa ttccgaaatc cttcctatta
      541 cggctaaaac aacacatttc atatttgatg ttgcaactgg acaaatgatt tacgagtttg
      601 aaactttaaa aagctataat aatgaaagta aaacggatga agtttactac tatcaattag
      661 ccatgaatga cattccgcaa atatataaag atgcgatgaa taagctgtac ctggatttgg
      721 gaaaaccgcc acgttgggca gattaaaggg atatggcttt ttccgttggc gaaaacctaa
      781 tcaaaaagtg gcccggtgcg ttcttcaaag acgcttataa tcgctttggc caattttttc
      841 gagtcatgtc gccagggata aagcccatca accaaatctg tttcatagac cttataagag
      901 cgtcggctgg tcgcgtcgtt gcgcacccaa tcgactcctt cacccaaatc gcctttaaag
      961 ttgttgttgc taatgaccaa atcgaaaatg tttttgccaa tatgctgttc taaaacgtgc
     1021 atatgatcag acacattaaa accgtccgtc tcgccccgct gcgtcgccac atttgacacc
     1081 aaaaagacct ccgcgcgact gctgcgtatg gcatcaacca aacccgacac caacagatta
     1141 ggtaagatgc tggtatataa actgcctggc ccgataagaa tcaaatccgc gttcaaaatc
     1201 gccgcgacgg ttggcggata agcttgcgcg tttgatggat caagcaaaac acgcaaaatc
     1261 ctgccattcg cccctgtaat tttgctttca cctttgatat gcacaatctc atcgctgtcg
     1321 tgcaagcgca catcacccac cagttgcacg ttactcagcg tagatggcaa taccttccca
     1381 taaatcgcca aaacacgccc ggattccgcc accgcttctt caaaactgcc ggtaatctct
     1441 gccagcgcgg tgataaacaa gttgcccaat gaatgcccct caagcccggc acccgatttg
     1501 aaacggtact gaaaaacctg cgaaagcaag gcttcatcgt tactcaaggc gcttaaacag
     1561 ttgcggatat cccctggggg caaaacgccc aaatcacggc gcagttcacc cgacgaaccg
     1621 ccatcatccg aaacggtcac gatggccgta atgttatgcg tgtaagcctt gatgccacta
     1681 agtaaagttg ccagtccatg cccgccacca atcgccacga ccttgatgcc gcggtctttg
     1741 cgccgatatt gctgcacggc ctcccaggcc gggcgtccct cgcgttcaaa aggtttgagc
     1801 accgcgcgat tagcccccca aatgccgaag agaatcagcg tgaaaccaat cccgccgaaa
     1861 agcagaaatc gaatgggacg gtcaagaaat cggagagata aaactgccag gataggcgtt
     1921 agaaattcgt tagtcgttgt gcggtagtat tgcaggacaa tcactgccag gcctaagccc
     1981 agcaacatcg cgccgatgac ggtcaaagcc agccatcgtt tatagccgag gccaggcaaa
     2041 aaccagcgga actcacggtt gagtttgtct gtaaaacgct tccaccagct cgttttcttc
     2101 ccgttatcgt gcatgaattg ataataactt ctttttaaac aggcgtcaat ctgttttgct
     2161 ttaccagcta cacattttcc attcagggca ttttataaag cttcgtctga gaaaaaatca
     2221 tagtcaattt tctttatctc ataagacgtg tttaacgtta cgcctacatt atagtcaatc
     2281 ataagttcag cgagtcgttc gccatcaatc aagattacct tggcgtcaat ttttttagca
     2341 taattaatgg cgtcctgcgt aaattttgct gtggtaataa aaacgcctct tcgggcatgt
     2401 tgaccaaata gtgcaccaac aaatttttga atttctggtc taccgattga agaatccgat
     2461 ttccaattct ttgcttgaat ataaatcgta tccagcccta acttatcctg gtcaataaca
     2521 ccatcaatcc cttcatcgcc atgccggcca actgcacggg ctaattcccg ctgagtgcct
     2581 ccatagccca ttttatgcaa gagttcaaca accagctttt caaaaaagcc aggtgacgtg
     2641 gatgtcacaa cgtttaaaac ttctgcagct aatgtctccc gtaactcctg gtatgctgat
     2701 tccattatct cttctggtgt ttcaattgaa tctgtttctg atttttctac tatctcatca
     2761 cttctttggg gtctcctttg tctaaactta acaaactcag ggtagcgaga tagtatttta
     2821 ttattaatag cttttggatt ttccgccagt aaatcaagcc ctctttgtgt tatcttaaag
     2881 taagttcgtc ttggatcttc caaaagttta gctgctttca tgtaagacct ggcccaacca
     2941 acacgattat aaaaaactga ctgagtaccg ctcggtagta atgccccttt ttcttcttcg
     3001 gttagcttga aataattact taattcttca actgcgtcat ggatatagtg ttcttttcca
     3061 tcagatgcca gttttaatag tggcagcatg attgtttgaa aatctggaat tgccatattt
     3121 gtcttcactc cttcagtcct ttaagttctc gcttatatca aacccgcacc ggcgcccggc
     3181 ctggcaacaa tcacatccat cacacccggc aaagctttga gcgcgggcag ggcgaaatca
     3241 agttcgtttt gcgtgccgat aacatgcaca ttcggacccg cgtccaccgt ggtacacagc
     3301 gcgtgcccct gctgacgcca gtagcgcact tgccacaaga tgagttcggt cagttccgtc
     3361 cagtagcgca aagaaggcgt gctggtgcgc atcaccgcgt gcatccactc cgagtccaac
     3421 tccgtcgccc aggcaaaagc ttcaaaatcg cgggccagta tcgcgttttt acagaccttt
     3481 aggcgttcgt ccacacccgc tacgcgcaat tcgttcagtg gcgaagtacg cgccagcgga
     3541 tgcccctgcg aagagcccac ctgcttatg
//
LOCUS       DAOSFC010000138         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24249_length_3568_cov_6.338457, whole genome shotgun sequence.
ACCESSION   DAOSFC010000138 DAOSFC010000000
VERSION     DAOSFC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24249_length_3568_cov_6.338457"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="PK791_04370"
     CDS             complement(<1..1341)
                     /locus_tag="PK791_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOH92581.1"
                     /translation="MSPKKLRFLYLCIAIVLIFGMLVGSQTVTANQPLPEIQVLIVPF
                     ENRDRLQILADRYDVVEVHHDTQTVKIFSSLNDRDALSRDGFSWTVDIPYTREINTVR
                     EKLPNQTSGMPGYPCFRTVDEMYARTAELASLYPDLVEIIDIGDSWQKINSGGTAGYD
                     IEVLLITNKNNPETPKSDTFYMSNIHAREWAPPEVNLRLAEFLLENYGQNPDITWMLD
                     YNRVHMVIVSNPDGRLIDEGGSNRMQRKNYNTNHCPGQYGNYNQTGVDLNRNYPFAWG
                     SGSNQCDLTYPGLSAGSEPETIAITTYLSGIFEDQRGPGENDPAPADTTGMFMDLHSY
                     SELVLYPWGYTYDNAPNHNQLKTVSYKLAYYNNYKPQKSTTLYPTYGSTDDFAYGEFG
                     IPAFCVEMGVTFYESCSRFESEIWPDNKLTLLTGIKLARRPYMDSYGPEVTFNSI"
     gene            complement(1711..>3568)
                     /locus_tag="PK791_04375"
     CDS             complement(1711..>3568)
                     /locus_tag="PK791_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOH92582.1"
                     /translation="VEVPLGNDYKYGDRISVYGELNQPPEGTTFSYRDYLFHKGIYAV
                     NQYGAVRLMSRGHGSRFLRALYALRERSNQVLQNNFPNPESALLRGILLGDESGISSE
                     LAQDYSRTGTSHIIAISGFNMVVLAGVVSAILKRRAGRLWDSLIAIAVLGAYTLFVGA
                     NPAVTRAFVMSAIAILGSSIARRGNLLNSLGLSVFVMVLIDPHLPWDIGFQFSVMATL
                     SLSLFATPLQAWVEEKLQKRFSEEKARNIASIISEYFLITLVAQILVLPLIIYHFREV
                     SWLFLLANPLILPVQPLVMILGLLALIGGLFSVPLGQFLSWLSWPFVAYTNKVVSGLA
                     RLAPNAWHLPSFSAWWLLVYYALVFLLAFRPKFKSSDKPIWQPVFALLGLGSLAIVLA
                     VHLASRPDGKLGIKVFNNPDAPIVLVRTPKGEMILSGGATSATRLAENVSKALPPFQQ
                     NIAYLIVPGCKKAQLNGFFGLPDQMRIGTVLWACDPESSRTAQSLFSYFENKDLSQLI
                     IDNQYLLNWSGGEMSFAIDEEGLQALMIIEADFSAVVDYDVATPSLRSDDLSLWVGKP
                     DDNTTTCAAIMLSTAESASKPEMQCPTEFVPVSEHDWVLVSTDGQQVWLRVK"
BASE COUNT          933 a          836 c          934 g          865 t
ORIGIN      
        1 gatgctgttg aaagtaacct caggaccata agagtccatg tagggtcggc gcgcgagctt
       61 gatacccgtt aagagtgtca atttattgtc tggccagatt tcgctttcaa atcgtgagca
      121 ggattcatag aaagttaccc ccatttcgac acagaaagcg ggaatgccaa actctccgta
      181 tgcgaagtca tctgttgagc catatgttgg gtatagtgtg gtggactttt ggggtttgta
      241 attgttgtaa taagccaact tataactcac cgttttcagt tgattatggt tgggcgcgtt
      301 atcgtaggta taaccccagg gatagagcac caattcgctg tagctgtgca ggtccataaa
      361 cataccggtt gtgtcagctg gagccgggtc gttttcaccg gggccacgct ggtcttcaaa
      421 gatgccgctc aggtaagtcg taatggcaat tgtttcgggt tcggatccgg cagaaaggcc
      481 tgggtaagta agatcgcact gatttgaacc gcttccccag gcaaagggat agttgcggtt
      541 aaggtccaca ccggtttggt tgtagttacc atattggcca ggacagtgat tggtgttgta
      601 gtttttacgt tgcatacggt tggagccacc ttcatcaatc aaacgcccat caggattgct
      661 gacgataacc atgtgcacac ggttgtaatc aagcatccag gtaatatccg ggttttggcc
      721 gtagttttca agcaaaaatt ctgccaaacg cagattgact tccggcggag cccactcgcg
      781 ggcgtggatg ttgctcatat agaaagtatc cgattttggc gtttccgggt tgtttttgtt
      841 ggtaatcagt agtacttcta tgtcgtagcc agctgtgccg ccactgttaa ttttctgcca
      901 gctgtcgccg atgtcgatga tttcaaccag gtcggggtaa agactggcga gctcggctgt
      961 acgcgcgtac atttcatcca ctgtgcggaa gcagggatat ccgggcatac ccgaagtttg
     1021 attaggcagt ttctccctga cagtgttgat ttctctggta taggggatat caaccgtcca
     1081 gctgaagcca tcgcgtgaca atgcgtcacg gtcattgagt gaagagaata ttttgacggt
     1141 ctgtgtgtcg tggtgaactt ccacaacgtc gtagcggtca gcaaggattt gcagacggtc
     1201 gcgattctca aagggtacga taagtacctg gatttcgggc aggggttggt tggcggtcac
     1261 agtttgtgag ccaacaagca tgccaaaaat aaggacaatt gcgatgcaga gatagagaaa
     1321 gcgcagtttt ttgggggaca tagaactcct tttgtcaata caaagtttca aagataggca
     1381 cgagtacaat atctcgtcct tgtggagaaa ttctaacgca taatggctta agcggggtga
     1441 atttaaacat tctcacttta taagttgagt atctggaaat aacggtgtta tatgagcggt
     1501 ttggcgcatt gagcttacaa aaaagtctga tttcttcaaa atcgtatggt aagcgtaggt
     1561 taagcgtcaa gcacgagaga taaaatagag ctaagataaa agcgatataa caatttctct
     1621 tcttcccctc cttaatactc aagtgccccg gtaggcgtct ggggcactta gtcgttaaat
     1681 aacaaagatt atggcagttt gcgtatcacc ttacttcacg cggagccaaa cttgctgacc
     1741 atcggtgctg acaagcaccc agtcgtgttc agataccggc acaaattctg tcgggcattg
     1801 catctctggt tttgaagcac tttcagctgt gctgagcata atcgccgcgc aagtggtggt
     1861 gttatcatca ggtttaccca cccacaatga caaatcatct gacctgaggc ttggtgtagc
     1921 aacatcgtaa tcaactacag cactaaaatc agcttctatt atcatcagcg cttgcagacc
     1981 ttcttcatca atcgcaaaac tcatttcacc gccggaccag ttgagcagat attgattgtc
     2041 gatgatgagc tgggagaggt ctttgttttc aaaataactg aagagcgatt gcgcggtgcg
     2101 agaactttca gggtcgcaag cccacaggac tgtgccgatg cgcatctgat ccggcaatcc
     2161 gaaaaagcca ttcagttgcg cctttttaca gccaggcacg ataagatagg caatgttttg
     2221 ctgaaaaggt ggcagagctt tggaaacgtt ctccgccaaa cgcgtcgcgc tggtcgcgcc
     2281 accgcttaga atcatctctc ctttgggcgt gcggactaaa acaatgggcg cgtccggatt
     2341 gttgaagact ttgatgccca gtttgccatc cggacggctt gccagatgca cggctaaaac
     2401 gatggcgaga ctgcctaatc ccagcaacgc gaaaaccggt tgccaaatgg gtttatcgga
     2461 tgatttgaac tttgggcgaa aggcgagcag aaaaaccagg gcgtagtaaa ccagcaacca
     2521 ccaggcgcta aagctgggca ggtgccaggc atttggtgcc aaacgcgcca gtccactgac
     2581 cactttgttg gtataagcca caaagggcca gctgagccat gacagaaact gtccaagcgg
     2641 aactgaaaac agaccgccga ttaaagccag cagacctaaa atcatcacca ggggttgaac
     2701 aggcaaaatg agcggatttg ccagcaggaa cagccaggaa acttccctga agtggtaaat
     2761 tatcagggga agcaccaaaa tctgcgcgac gagagtgatc aaaaagtact cgctgataat
     2821 gctggcaatg ttgcgcgctt tttcttcgga aaaccgtttt tgcaattttt cttccaccca
     2881 ggcttgcaaa ggtgtggcga acaaggaaag cgataaggtc gccatcaccg aaaactgaaa
     2941 gccgatatcc cagggcagat gcgggtcaat cagcaccatg acaaagacac tcaagcccag
     3001 gctgttgagc agattgcccc gcctggcgat gctgctgccc aaaatcgcga tagcactcat
     3061 cacaaaggcg cgcgtaaccg ccgggttggc gcccacaaaa agcgtataag ccccaagtac
     3121 agcgatggca atgagactat cccacaaacg acccgcgcga cgttttaaaa tcgcgctgac
     3181 cacccccgcc aaaacgacca tgttgaaccc ggaaatagca atgatgtgcg acgtgcccgt
     3241 tcgcgaatag tcctgggcga gttcgctgga aataccactt tcatcgccga gcaaaatgcc
     3301 acgcagcaga gcgctttcgg ggttggggaa gttgttttgc aagacctgat tgcttctttc
     3361 gcgaagggca tataaagcgc gcaggaaacg gctgccatgc ccgcggctca tcagccgaac
     3421 cgcgccatat tgattcacgg cgtagatgcc cttatggaag agatagtcgc gataagagaa
     3481 agtggtccct tcgggtggtt ggttaagctc gccgtaaaca ctgatgcggt cgccgtattt
     3541 gtagtcattg cccaaaggga cttcaacc
//
LOCUS       DAOSFC010000139         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24308_length_3562_cov_5.114913, whole genome shotgun sequence.
ACCESSION   DAOSFC010000139 DAOSFC010000000
VERSION     DAOSFC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24308_length_3562_cov_5.114913"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /gene="uvrC"
                     /locus_tag="PK791_04380"
     CDS             complement(<1..1630)
                     /gene="uvrC"
                     /locus_tag="PK791_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOH92583.1"
                     /translation="MKNEKDEIIYVGKAINLKNRVRSYFQKTASQNFKTRQLVKAIRD
                     IDWIVVGSELEALILELNLIKQHRPFYNVKLKDDKRYPYIKVSWADPFPKLTVTRQMD
                     NDGSRYFGPYTSVWAVHQTLDVLRKIFPVLSCNRHITGKDTRPCLYYDLKMCLGPCIG
                     AVDQESYRQVVDDLCKFLEGKTDAIMKRLKSDMAQHSDALNYEKAATIRDQINAIERV
                     VERQKIVSDSQQDSDIIAMARGDNEACVQVFFIRSGKMIGREYFILEGTNDEENSEVL
                     EEFIKQFYSQAAFIPKNVLLPQDVDEANIIREWLNTKRGGQKVEIKVPKRGKWRELVE
                     MATENAKETLTALETRWKADKDRQKEALAQIQSALDMPAPPNRIECFDISNIQGMASV
                     ASMVVFERGVANKKLYRRFNIRGVTGPNDYASMVEALHRRFANYEEALQHKDEPGKKQ
                     DLAFSLLPDLLIVDGGKGQLSAALEVIKNFSLEDKFFIAALAEKQDKLFIPNQSDSIR
                     LDDGSQGLFLLQRIRDEAHRFAITSHRNRRGKIGM"
     gene            1761..3533
                     /gene="mutL"
                     /locus_tag="PK791_04385"
     CDS             1761..3533
                     /gene="mutL"
                     /locus_tag="PK791_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOH92584.1"
                     /translation="MKIKVLSEQLASQIAAGEVIERPSSVVKELIENAIDAGATQIEL
                     EIKDAGKKLIQVSDNGVGIPADEIALAVSRHATSKLSKAEDLFDIRTLGFRGEALSSI
                     GSVAKLSIVSKHRDAELGAEVSVDGGKTEGVKAKSAPDGTVVRVEDLFFNVPARLKFL
                     KTNTTENRQITGLITRYALAYPHIRWQLVQDGRVYLQTTGSGDRKEILQSLFGVSDAK
                     RMLPILFEDGEMRIEGFISELGFTRSNRRDITLFVNGRWVQDASLTAAVIRAYNTMLM
                     VGRFPVVVLFIQMPPDMVDVNVHPSKAEVRFKESDQVFSTVQRAIRRGLLAYSPVPEL
                     NTAVLWGRPVEDISTSKPVQMEAPWQMPDQQHEKTEESERVQTGFQPVGQTQSLQAPL
                     SSDYLPVLRLVGQVAATYLIAEGPDGLYLIDQHAAHERVLFEQMMAQARSGIVPSQAL
                     LEAAVVELTPEKAGILEERFDVLKGLGFEIENFGPNTFALRAVPAILAGGDPAEAIYA
                     VVNAFEEDETPLMEEIQDLVAARVCKRMAIKAGQILSPEEQKNLLLNLETCQVPRTCP
                     HGRPTMIHLSAMMLERQFGRTGAI"
BASE COUNT          912 a          769 c          894 g          987 t
ORIGIN      
        1 tcatgcctat tttaccacgg cgatttctgt gagaagtgat agcgaaacga tgcgcttcgt
       61 cgcgaatgcg ctgcaagaga aacaggcctt gcgaaccgtc atcaagtcga atcgaatctg
      121 actggttggg aataaaaagt ttatcttgtt tttctgcaag ggcggcaatg aaaaacttat
      181 cttctaaact gaaatttttt atcacttcta aagcagccga aagctgtcct tttccgccgt
      241 caacgattag caaatcgggc aaaagactaa aagccaaatc ctgttttttg ccgggttcat
      301 ctttgtgttg caaggcttct tcgtaattcg caaaacgacg gtgtagggct tcaaccattg
      361 aggcgtaatc atttgggcct gttacaccgc ggatgttgaa gcgccggtaa agcttcttgt
      421 tggcaacgcc acgttcaaaa acgaccatgc tggcgacgct tgccatgccc tggatattgg
      481 agatgtcgaa acactcgatg cggttgggtg gggcaggcat atctaatgcg ctttgtattt
      541 gtgccagagc ttccttttgg cggtctttat cagctttcca gcgggtttcc agggcggtaa
      601 gcgtttcttt ggcgttttct gttgccattt ctactaattc gcgccatttt ccgcgcttgg
      661 gtaccttgat ttcaactttc tgaccacctc gtttcgtgtt tagccattcc ctgatgatat
      721 tggcttcatc gacatcttgc gggagaagga catttttggg gataaatgca gcctgggaat
      781 agaattgctt gatgaattct tccaaaactt cactgttttc ttcgtcgtta gtgccttcaa
      841 gaatgaagta ttctctgcct atcattttac cactacgaat gaagaacact tgcacacagg
      901 cttcgttgtc gccccgtgcc atggcaatga tgtcggagtc ttgttggcta tccgagacaa
      961 ttttttggcg ttcaaccacg cgttcaatcg cgttgatttg gtcgcggatg gtggcggctt
     1021 tttcgtaatt aagcgcgtct gaatgttgcg ccatgtcgct tttgaggcgc ttcatgatgg
     1081 catcggtttt gccttccaaa aacttgcata aatcatccac aacctgacgg tagctttcct
     1141 ggtcaaccgc tccgatacaa gggcctaaac acatttttaa atcataatac aaacaaggac
     1201 gggtgtcttt gcccgtgata tgtcgattgc aactcagtac agggaatatc tttcgcaaca
     1261 cgtccagggt ctgatgcact gcccaaacgg atgtgtatgg tccaaaatac ctggatccat
     1321 cgttgtccat ctggcgggta acggttagct ttggaaaagg atccgcccag ctgactttga
     1381 tgtaaggata gcgtttgtca tcttttagtt tgacgttgta aaagggacgg tgttgcttga
     1441 tgaggttgag ttcaaggata agcgcttcga gttctgaacc gacaacaatc cagtcaatat
     1501 cgcgaatggc cttaaccagc tgacgggttt tgaaattttg cgatgcggtt ttctgaaaat
     1561 atgaacgcac gcgatttttg agattaatcg cttttccgac gtagataatc tcatcttttt
     1621 cgttcttcat cagatagcat ccaggcttat cgggcaatgt gcataggatg tcttgaatgt
     1681 gctgttcata gttcatgaca tcattttaac atcaaggtat acaaacttta taaaataaat
     1741 cgacttggta taataaaact atgaaaataa aagtactttc cgaacaactt gcatctcaaa
     1801 ttgccgctgg ggaggtgatt gaaagacctt catcagtggt aaaagagctc attgaaaacg
     1861 caattgacgc cggtgcgaca caaatcgaac tggaaataaa agacgcaggc aaaaagctta
     1921 tacaagtttc cgacaatggt gttggtatcc ctgccgatga aattgctttg gctgtatcgc
     1981 gccatgcgac gagtaagctt agcaaagccg aagatttatt tgacatccgt accctgggtt
     2041 ttaggggtga agcattgtct tcaatcgggt ctgtggcaaa actgtccatt gtcagcaagc
     2101 atcgggatgc tgaacttggc gcagaagtca gcgttgatgg gggcaaaact gaaggtgtaa
     2161 aagcaaaaag cgcgcctgat ggaactgtgg tgcgggttga ggatttgttt ttcaacgtac
     2221 ctgcccgact aaagtttttg aaaacgaata cgactgaaaa ccgacagata actggcttaa
     2281 ttacgcgcta tgccctggct tatccacata ttcgctggca actggtccag gatgggcggg
     2341 tgtatctgca aacgactggc agtggtgacc gcaaggaaat cttgcagagt ttgtttggtg
     2401 tatcggacgc caaacgaatg ttgcccattc tttttgaaga tggcgaaatg cgcattgaag
     2461 gtttcatcag tgaactgggc tttacgcgtt caaaccgacg cgatattacg ttgtttgtca
     2521 atgggcgctg ggttcaggat gcgtcactta ccgcagcggt aatcagggca tataacacca
     2581 tgctgatggt tggacgtttt cccgttgttg ttttgtttat tcaaatgcca cccgatatgg
     2641 ttgatgtgaa cgtgcatcct tccaaggctg aagtgcggtt taaggaaagc gaccaggtct
     2701 tcagtactgt tcaacgcgca atcaggcggg gacttttggc atactcgcca gtacctgaac
     2761 tcaatacagc tgttttgtgg ggtcgtccag ttgaagatat ttccacatca aaaccagtac
     2821 agatggaagc cccatggcaa atgcctgacc agcagcatga aaaaacagaa gaatcagaaa
     2881 gggtgcaaac tggttttcaa ccagtcggac aaacccaaag cttacaggca cccttatcta
     2941 gcgattatct gcctgtttta aggctggtag ggcaagttgc ggcgacctat ctcatcgctg
     3001 aagggccaga tggcttgtat ttaatcgacc aacatgctgc ccatgaacgg gtgcttttcg
     3061 aacaaatgat ggcgcaggca cgctcgggca ttgtgccatc gcaggcttta ctggaagcag
     3121 ctgtggtaga actgacacct gaaaaagccg gaatcctgga agaacggttc gatgtgctaa
     3181 aaggcctggg ctttgagatt gaaaattttg gaccgaacac cttcgcattg cgcgctgttc
     3241 ctgccatttt ggctgggggt gatccggcag aagccattta cgctgtggtg aatgcttttg
     3301 aagaagatga gacgccatta atggaagaaa ttcaggattt ggtggctgcc agggtgtgta
     3361 aacgtatggc gataaaggca gggcagatat tgagccctga agaacaaaaa aatcttttac
     3421 tcaacctgga aacctgtcag gtgccacgca cttgtccgca tggtcgtcca acgatgattc
     3481 atctcagcgc gatgatgctt gaacgacaat ttggtcggac tggggcgatt taatcacagg
     3541 agctgacgat gaaagcatta tg
//
LOCUS       DAOSFC010000140         3555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24361_length_3555_cov_5.118000, whole genome shotgun sequence.
ACCESSION   DAOSFC010000140 DAOSFC010000000
VERSION     DAOSFC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24361_length_3555_cov_5.118000"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..814)
                     /gene="rplA"
                     /locus_tag="PK791_04390"
     CDS             complement(92..814)
                     /gene="rplA"
                     /locus_tag="PK791_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOH92585.1"
                     /translation="MSKKGKNFANAIKKIEAEKAYEPSEGLALVKEMAFAKFDETVEV
                     HIRTGLDPRQADQQIRDTVVLPHGLGKDVRVLVFAQGEAATAAREAGAEFVAETDEDL
                     ARIQGGYTDFDVAVGTMDMMGKVGRLGRILGPRNLMPNPKAGTVVQPDDISEAIRLAK
                     AGRVEFRLDKSANIHVPIGKVSFYTEKLYDNMAALMNAVVRARPSGAKGAFIRRVALA
                     STMGPGVKVDVASATSMKASQV"
     gene            complement(873..1301)
                     /gene="rplK"
                     /locus_tag="PK791_04395"
     CDS             complement(873..1301)
                     /gene="rplK"
                     /locus_tag="PK791_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOH92586.1"
                     /translation="MAKKVKAVVRLQIQAGKANPAPPIGPALAPHGVNIMAFCKDYNA
                     RTSNRMGEIIPADITIFADGSFRFELKSPPAAVLLKKAAGIAKGSATPQSEKVGKVTR
                     EQLKEIANLKFKDMNANDIESAMKQLEGTARNMGITVVDK"
     gene            complement(1366..1989)
                     /gene="nusG"
                     /locus_tag="PK791_04400"
     CDS             complement(1366..1989)
                     /gene="nusG"
                     /locus_tag="PK791_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOH92587.1"
                     /translation="MTLENDAPLVDDQSESTTGQTGLEQGASKSKRDERRQWYVIHCY
                     SGYENKVRKNLEQRVETMGMKDRIFDVVIPTQEEIEVRDGKRRTVERHVFPGYVLVEM
                     ILEEESWFVVRNTPGVTGFVGMGDEPTPLRDDEVTHIMRRMESEAPTYKVTYKAGDRV
                     RIVDGPFNDFRGTVDEIDMERSKIRVMVNFFGRETPVELDFLQVEKA"
     gene            complement(2010..2222)
                     /gene="secE"
                     /locus_tag="PK791_04405"
     CDS             complement(2010..2222)
                     /gene="secE"
                     /locus_tag="PK791_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOH92588.1"
                     /translation="MAKTSGKPNIFQKIRRWWRETVGELRKVTWPTFKEALALTRVVL
                     IVTVIMAAILGLLDFVFARLVGLLIG"
     gene            complement(2280..2352)
                     /locus_tag="PK791_04410"
     tRNA            complement(2280..2352)
                     /locus_tag="PK791_04410"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2317..2319),aa:Trp,seq:cca)
     gene            complement(2377..2547)
                     /gene="rpmG"
                     /locus_tag="PK791_04415"
     CDS             complement(2377..2547)
                     /gene="rpmG"
                     /locus_tag="PK791_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOH92589.1"
                     /translation="MAKKGKDIRPVIHLACTECRERNYTTVKNRRNDPGRLELNKFCP
                     RCRAHKLHREVR"
     gene            complement(2609..>3555)
                     /locus_tag="PK791_04420"
     CDS             complement(2609..>3555)
                     /locus_tag="PK791_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOH92590.1"
                     /translation="YIKNMITGAAQVDGAILVVSAPDGPMPQTAEHVLLARQVEVPSI
                     LVFLNKTDQMDDPELLELVDMEIRELLNDNGFPGDDTPIIKGSALVALESTSTDPNAP
                     EYQPIIELMQAVDDFFPEPVRELDKPFLMPIEDVFSIKGRGTVVTGRIERGQLKLNDP
                     VEIVGLMDKPLATVATGIEMFHKLLETGNAGDNAGILLRGIDRDQVERGMVLCKPGSI
                     TPHTEFEASTYILTKEEGGRHSAFFSGYRPQFFINTTDVTGDVTLPEGVEMVLPGDTV
                     DGLRVKLMEKVALEEGSEFAIREGGLTVGAGKITKIIA"
BASE COUNT          828 a          919 c          874 g          934 t
ORIGIN      
        1 agctttcttt agcagagcta atattcaatt gttcagtcgg gtagtatacc acagttcgcg
       61 cggaatacta ccccgattct cttgcaaatg actacacttg cgaagctttc attgaagtcg
      121 cgctggcaac gtcaaccttc acaccagggc ccatagttga agccagggct acacggcgga
      181 taaaagcacc tttcgcgccg gaaggtcgcg cgcgaacaac agcgttcatt aaagccgcca
      241 tgttatcgta cagtttctcg gtataaaaag aaactttgcc aataggcacg tggatgttcg
      301 cggatttatc caggcggaat tccacacgtc cggcttttgc caagcgaatg gcttcggaaa
      361 tgtcatctgg ttgcacaacg gtaccagctt tggggttagg catcaagttg cgggggccca
      421 aaatacgacc caaacggcca actttaccca tcatatccat ggtaccaact gccacgtcaa
      481 aatcggtgta gccaccctgg atacgggcaa gatcttcatc agtttctgcc acaaattccg
      541 cgccggcttc gcgggcagcg gtggcagctt cgccctgggc gaaaactaaa acgcgcacgt
      601 ctttacccaa accgtggggc aaaacgacgg tatcgcggat ttgctggtca gcctggcggg
      661 gatccaaacc ggttcgaatg tgaacttcaa cagtttcgtc aaatttggca aatgccattt
      721 ctttgacgag cgcgagccct tcgctgggct cgtaggcttt ttcagcctct atttttttga
      781 ttgcgtttgc aaaatttttg ccttttttag acatgaatac tcctttgtgg tcctgacggg
      841 cgttttagcc ctcccacact gactaattaa acttacttgt caactaccgt aatacccatg
      901 ttgcgggcag taccttcaag ctgtttcatc gcggactcaa tatcattcgc gttcatatct
      961 ttaaatttaa gattagcgat ttccttgagt tgctcgcggg ttaccttgcc aaccttttcg
     1021 ctttgcgggg tcgcggaacc tttggcgatg ccagcggctt tcttgagaag cacggctgct
     1081 ggcggtgact tgagttcgaa gcggaaggac ccatcagcga aaatggtgat atcggcaggg
     1141 ataatttcgc ccatgcggtt ggatgtgcgg gcgttgtaat ctttacagaa cgccataatg
     1201 tttacgccat gtggcgcgag cgccggaccg attgggggcg cggggttggc cttgccggcc
     1261 tggatttgta gtctaactac tgcttttact ttctttgcca ttcattaact cctttgtggt
     1321 gcatacgggc ttttaaccct cccacgtcat tccggtataa ccggtttatg ccttttctac
     1381 ctgcagaaaa tcaagttcca caggggtttc tcgcccaaag aaattcacca tgacgcgaat
     1441 ctttgaacgt tccatatcaa tttcatcaac ggtgcctcta aagtcattga aaggcccatc
     1501 aacaatgcgc acacgatcgc ccgctttata agtgacctta taagttgggg cttcgctttc
     1561 cattcggcgc ataatgtgag taacttcgtc atcgcgcaaa ggcgtaggct catcacccat
     1621 gcccacaaac cctgttacac caggggtatt gcgcacgacg aaccaggatt cttcttcaag
     1681 aatcatctcc accagcacat agccaggaaa aacatggcgc tcaaccgtgc gacgtttgcc
     1741 atcgcgaacc tcaatctctt cttgcgttgg gataacgaca tcaaagattc tatctttcat
     1801 gcccatggtt tcaacgcgtt gctcgagatt tttgcgcact ttgttttcgt agccagaata
     1861 gcaatgaatg acgtaccatt gacgtcgttc atcgcgcttt gactttgacg cgccttgttc
     1921 aagcccggtt tgtccagtgg ttgattctga ctgatcgtca accagcggag catcattttc
     1981 cagagtcatt tcctgctcgc aactctcaat taaccaataa gtaaaccaac caaccgagcg
     2041 aagacaaagt ctaacaggcc aaggattgct gccatgataa cagtaacaat caacacgaca
     2101 cgtgtcagag ccagtgcttc tttaaaagtt ggccaggtaa ccttgcgcaa ctcgccgacg
     2161 gtctcacgcc accagcgtct tattttttga aaaatatttg gcttaccgct tgtttttgcc
     2221 atagcatttc tcctctagag ttcaacgccg tagtagctac ggcgtcaaat caacatcaac
     2281 aggtcaggaa ggactcgaac cctcagcccc cgcttttgga gagcggtgct ctgccaattg
     2341 agctactgac ctaggtactt tctttattaa agaaagttat ctcacctcac ggtgaagttt
     2401 atgcgcccga caacggggac aaaacttgtt cagttccaaa cgtcccgggt cgttacgtcg
     2461 gttcttgacc gtggtgtaat tgcgctcgcg gcactccgtg cacgccaggt gaattactgg
     2521 tcgaatgtct ttaccttttt ttgccatctt attctaacat ggctgcctca atggaggcag
     2581 ccatgtatcc tccgtttata gattcttact aggcaataat cttggtgatc ttgccagcgc
     2641 caacagtcag gccaccttcg cggatagcga attcggaacc ttcttcgagg gcgacttttt
     2701 ccatcaactt gactctgaga ccgtcaactg tatcgccggg cagaaccatt tccacgcctt
     2761 cgggcagggt aacgtcgccg gtgacgtcgg tggtgttgat gaagaattga ggtcgataac
     2821 cgctaaagaa agcgctgtgg cgtccacctt cttccttggt caggatatag gttgaagctt
     2881 cgaactcggt gtggggagta atggagcctg gtttacagag aaccatacca cgttcgacct
     2941 gatcgcgatc aataccgcga agcagaatac ccgcgttgtc accagcgtta ccggtttcga
     3001 gcagtttgtg gaacatttcg ataccagtgg caacggtggc caggggtttg tccatcagac
     3061 caacgatttc aacggggtcg ttcagtttca gttgaccgcg ttcgatacga ccggtcacaa
     3121 cagtaccgcg acctttgatg ctgaagacgt cttcgatagg catcaagaag ggtttgtcca
     3181 gttcgcgaac aggctcgggg aagaaatcat ccacagcttg catcagttca atgatgggct
     3241 gatattctgg agcgttgggg tcggttgagg tgctttcgag cgcgacgaga gcggaacctt
     3301 tgatgatggg ggtatcatcg ccggggaagc cgttgtcgtt gagcagctcg cgaatttcca
     3361 tatcaaccag ctcgagcagt tcggggtcgt ccatttggtc agttttgttc aagaagacca
     3421 aaattgaggg aacctcaacc tggcgggcca aaagcacgtg ttctgcggtt tggggcatgg
     3481 ggccatcagg agcgctgaca accaaaatcg cgccgtcaac ctgggctgca ccggtgatca
     3541 tgttcttgat gtagt
//
LOCUS       DAOSFC010000141         3551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24391_length_3551_cov_6.133009, whole genome shotgun sequence.
ACCESSION   DAOSFC010000141 DAOSFC010000000
VERSION     DAOSFC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24391_length_3551_cov_6.133009"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PK791_04425"
     CDS             complement(<1..614)
                     /locus_tag="PK791_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92591.1"
                     /translation="MSRKNALVPVLLILTCLLLVNTACEPIYEGRPTGFPTQLPTNTV
                     TPTQAATPTEAPTVTHTLTITPTDTPTPIPTATPTSTPTVIPTRESGLIQLTVGENTT
                     VDWGYHYLTQNNKRDDNSQYNLSAMLAFQLIDRGIHSETITVLGNDITVYYLRARHAF
                     KGEMMDVKIILTGIFGTDVAIDTMPADGANFVYYRTQNADVPFEP"
     gene            complement(607..1485)
                     /gene="amrB"
                     /locus_tag="PK791_04430"
     CDS             complement(607..1485)
                     /gene="amrB"
                     /locus_tag="PK791_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOH92592.1"
                     /translation="MSSEQVDVRPSPIAGTWYPGTETELRRAMERFFADEGNKFISPV
                     GDLLGLVVPHAGYYYSGACAAAAFQALRGKNYQKVIIISPSHRAYTAPLLTSGHDAYE
                     TPLGLVTVDHLALNQLNTILNNHHLGLKTVRFDQEHALEIELPFLQFTLGNDFEILPI
                     MMVDQRENTAQALALALFELIQSLDSSEKVLLVASTDLSHFHHQRVAQKLDRAFIDAL
                     QTGDPKQLYRAANTGKTEACGLGPTACVLETCQLLGANAITITDYRDSSCAGGDKSSV
                     VGYVSALITREDKANV"
     gene            complement(1597..2805)
                     /locus_tag="PK791_04435"
     CDS             complement(1597..2805)
                     /locus_tag="PK791_04435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH92593.1"
                     /translation="MDSQEPLASDSINATDENTSPESESWLHKTWEQLVRLGLHDTAR
                     RVVAMISTMLFMVLAILFMNRFFVSHSGQKPSELGSSPSSLAESSGLALLPDFRIDND
                     QAEKHSIPRDTNMVTILPERVRKGIVEHTIQPGESIFSIAAKYGLRPETLLWSNRYTL
                     GDDPHIMTPGQTLLILPIDGILHRWSAGEGLNGVAEYYHVSPEAIVSYAPNGLSMASI
                     GDLADPNIQPGTLLIIPGGYAEFSDWRTPRITRKEPATAKNVGPGACEGAYDGVVGAE
                     KFTYPVQGEAWLSGFDFQPAANHFGIDLGGAIGDPISAVDNGVVVYAGWNDWGYGEMV
                     VIDHGNGWQSLYAHLATVDVRCGQEVYRGDQLGTMGQTGNAVGPHLHFELRHDEYGRV
                     NPWDFLQTLD"
     gene            2951..>3551
                     /locus_tag="PK791_04440"
     CDS             2951..>3551
                     /locus_tag="PK791_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92594.1"
                     /translation="MKKRNLLIVILIITLLLGACKAKTNGDTTPDPVNPDATQAPVEA
                     SKVPTHTPRYLGPIYQTKIFLGGHGWANNVDKTAFYHTANFGEHWVTATPPDLAKQMH
                     DYPLISSFFYSGNTGYILLSRPEDKSLLYRTGNGGETWETTELDFPGGQMYFISSTEG
                     YMLSSLGVAAGSEYIAINKTTDGGKTWQTVFSHEPGQVSD"
BASE COUNT          891 a          903 c          884 g          873 t
ORIGIN      
        1 ggttcaaacg gcacatctgc gttttgggtg cggtaataaa caaagttcgc tccatcggca
       61 ggcatcgtat caatcgccac gtcggttcca aaaatacccg tcagaatgat tttcacatcc
      121 atcatttcgc ctttaaaggc atgccgcgcg cgtaaataat aaacggtaat atcattaccc
      181 aaaaccgtaa tcgtttcgct gtggataccg cgatcaatga gttgaaaagc cagcatcgct
      241 gaaagattgt attgactgtt atcgtcccgc ttgttgtttt gggtcaggta atgataaccc
      301 cagtccacag tggtattctc gccaactgtg agttgaatca agcccgattc acgggttgga
      361 ataacggtgg gtgtgctggt gggcgtggca gttgggatgg gcgtcggcgt atcggtgggc
      421 gtgatagtga gtgtgtgggt caccgttggc gcttctgttg gcgtggctgc ctgggttggc
      481 gtgacggtgt ttgtgggaag ttgcgtgggg aagccagtcg gacgcccttc atagataggt
      541 tcacaggcgg tattaaccag caatagacaa gtaagtatta aaagaacagg cacaagagcg
      601 ttttttctag acattcgctt tatcctcacg cgtaataaga gccgaaacat aacctaccac
      661 cgagctttta tcgccaccgg cgcaggatga atcgcggtaa tccgtaatcg ttatcgcatt
      721 tgcgccaagc aactggcagg tttcaagcac acaagccgtg ggtcccaaac cacaagcttc
      781 cgttttgcca gtgtttgcgg cgcggtaaag ttgctttggg tcgccagtct ggagggcgtc
      841 aataaaagcc ctgtccagtt tttgcgcgac gcgttgatga tggaaatggg aaaggtctgt
      901 tgatgccacc aacaatactt tctcggatga atcaagactt tggattagct caaacaatgc
      961 cagcgccaaa gcttgcgcgg tattttctcg ttgatcaacc atcataatgg gcagaatttc
     1021 aaaatcattg cccaaagtaa attgtaaaaa gggcagttca atctccaggg cgtgttcctg
     1081 atcgaaacgc acggttttta gaccaagatg atgattattc aagatagtgt ttaattgatt
     1141 tagcgcgaga tggtcaacgg tcaccagacc taagggtgtt tcgtaagcgt catgcccgga
     1201 tgtaagcaaa ggagcggtat aggcccgatg ggaaggggag atgatgataa ctttttgata
     1261 gtttttccca cgcaaggcct gaaacgccgc tgcagcgcaa gccccagagt aataataacc
     1321 agcgtggggt accaccaaac ccagcaagtc tccaactggc gagataaact tattaccttc
     1381 atcagcaaaa aatctctcca tggcacgtcg cagttcagtt tcagtgcctg gataccacgt
     1441 tccagctatg ggagaaggtc tgacatcaac ttgctcactg ctcataggct tattttaaca
     1501 cgataatcag gacttggcag gattgattgc gggttttgcc tgccgtgcat aagatgcctt
     1561 tgccgttatt ttcgggataa tgtttttact tgagaatcag tcgagtgttt gcaaaaagtc
     1621 ccaggggttg acgcgcccat attcatcatg gcgcaactca aaatgcaagt gcgggccaac
     1681 cgcgttgccg gtttgcccca tggtgccaag ctgatcgccc cgataaactt cctgtccgca
     1741 acgcacatcc accgtcgcca gatgcgcgta aagactctgc cagccgttgc cgtggtcaat
     1801 gaccaccatc tcgccgtagc cccagtcgtt ccagccagcg tataccacca cgccattgtc
     1861 caccgcgcta atcgggtcac cgatggcgcc gcccaagtcg attccaaaat ggttggcagc
     1921 cggctgaaag tcaaatccgg aaagccatgc ttcgccctgt accggatagg tgaatttttc
     1981 cgcgccaaca acgccatcat acgccccttc gcaagcgcca gggccgacat tcttcgccgt
     2041 ggcaggttct ttgcgggtga tgcgtggcgt gcgccagtcg ctgaattcag catagccacc
     2101 tgggataatc aaaagcgtac cgggttgaat attcggatct gctaaatcac cgatgcttgc
     2161 catgctcagc ccgtttggcg catagctgac aatcgcctcc ggactcacat gataatactc
     2221 agccaccccg ttcaggcctt ctcccgcgct ccagcgatgc aagatgccat caatgggcaa
     2281 aatcaacagg gtttgacccg gggtcataat gtgtgggtcg tcccccaggg tatagcgatt
     2341 gctccaaagc aaggtttcag gtcgcaggcc atatttcgca gctatcgaaa agatactctc
     2401 gccgggttga atggtatgct ccacaatgcc cttacgcacc cgctctggca atatcgtgac
     2461 catatttgta tcacgcggga tactgtgctt ttcagcctgg tcattatcaa ttctaaaatc
     2521 tggcagcaag gctaagccgc tgctttcagc cagcgatgac ggactgctgc caagctcgga
     2581 tggcttttgc ccgctgtgac tgacaaaaaa acgattcata aacagaatag ccaaaaccat
     2641 aaaaagcatg gtgcttatca tcgccacaac gcgccttgcg gtatcgtgca agccaagacg
     2701 caccagctgt tcccaggtct tgtgcaacca gctttcgctt tcgggcgaag tgttttcatc
     2761 ggtcgcgttt attgaatcgc ttgctaaagg ttcttgcgag tccattcata ttgatgatat
     2821 cacgagtcaa taaattcgat gtcctgcacg ctatggcttt aggtggtaaa atgcgtgggt
     2881 gaatttttca cgctgtacta taatgaatac aagaatttga aagcacgacc tgctttttgg
     2941 aaggattttt atgaagaaga gaaacttgct catcgtcatt ttgataatta cactgctttt
     3001 gggcgcttgc aaagctaaga caaatggtga cacgacgcca gacccggtaa atccggatgc
     3061 cactcaagct cccgtcgaag caagcaaagt cccaacgcat accccgcgct atcttggccc
     3121 tatttaccaa accaaaattt tcctgggtgg gcacggttgg gcgaataacg ttgataaaac
     3181 tgctttttac cacaccgcga actttggcga gcactgggtc accgctacgc cacccgattt
     3241 agccaagcaa atgcacgact atcccttgat ttcaagcttt ttctacagtg gaaatacggg
     3301 ctatatcctg ctcagccgcc ccgaagacaa gagtttgctt tacagaaccg gcaacggtgg
     3361 cgaaacctgg gaaacgaccg aactggactt tcccggcggg caaatgtatt tcatcagtag
     3421 caccgaaggc tatatgcttt cgagtttagg cgttgctgct ggctcggaat atatcgcgat
     3481 caataaaacc accgacggtg gcaaaacctg gcaaaccgtc ttcagccacg aacccggaca
     3541 ggtttctgac a
//
LOCUS       DAOSFC010000142         3538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24516_length_3538_cov_4.933391, whole genome shotgun sequence.
ACCESSION   DAOSFC010000142 DAOSFC010000000
VERSION     DAOSFC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24516_length_3538_cov_4.933391"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1508
                     /locus_tag="PK791_04445"
     CDS             156..1508
                     /locus_tag="PK791_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92595.1"
                     /translation="MSHSMEKQDRIQWGKLFLIGFGFFGVSVLWSLYNTYVPLLLDAK
                     FGLDSVAISIIMVLDNIAAFLIQPAVGVWSDNLRTKIGRRLPFIIVGAPLAAVAFGLI
                     PLAQTLPVLLACTVTTLLAMAFWRTPVVALMPDVTPSKFRSQANGVINFMGGLGLVLG
                     TSVGAALVKMDHPLAGAFPFWFGSILVILAAVLVFIFVREPEYEKHEGEEKPKSASTL
                     DGLKEVFTGKQRDAILIFLAIFFWFIAYNAIETFLSLYGRNFLGLSDSGAGQLITSVG
                     ASFLLFAIPAGLIGAKFGRRRTILTGLAIMVLTMSSMFFIPKDILLTTHGRIPILGDF
                     MNVTIILLLAGIGWALININSLPMVVDLTTKDRVGTYTGLYYFFSTLSAIIGPLLNGL
                     IVKLSGGDYGQIMLVGPIFMALAFLAIVFVKRGEINPEADRRHAKDYTEEYEGIDAIL
                     "
     gene            1667..2710
                     /locus_tag="PK791_04450"
     CDS             1667..2710
                     /locus_tag="PK791_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOH92596.1"
                     /translation="MKKRLLSILAILLVAGLVLGACTPKTPVVDEAPEGDVVDQPTET
                     FKVGLVTDVGVVDDKNFNQATWEGVLAAQKELGFEAKYIETKDAKDYDANIQLFVDEG
                     FDVIVTVGFALGEATINAAKANPEIAFIGVDQFQGEVVPGVAGLIFDEALSGVLAGVL
                     AANLTETKTIAAVLGTDLVPPVVNYNRGYVGGAKWFDPEISIISTYHPGGMDVAFTDP
                     EWGASTAKQAIEQGADIVFAAAGNTGNGALIETAQHDGLFCIGVDTDQWLTVPEAHKC
                     LVSSATKDITLSVVELIKLAYEGNFPSGNYVGGSSLAPFHDFESIITEEIQAKLKEAL
                     EAYNAGKIELPEK"
     gene            2799..>3538
                     /locus_tag="PK791_04455"
     CDS             2799..>3538
                     /locus_tag="PK791_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH92597.1"
                     /translation="MEEQTPSQNNWLKKVWFSVRIPFLAIILALLVGAIILLVSGANP
                     LEAYWQLFKGAFGSWRNIQRVLEKSTPLIFAGLAVALGFKAGLFNIGAQGQLLLGALM
                     AGYVGFAVKGLPAYIHIPLALLAGLLTGAVFAAIPGLLRAYSGAHEVISTIMLNYVAI
                     NLSDYFSEGPWKDTTPGNIVSRLPAIQPSAEIPNIGPVPVGFIVAVLTSLLIWFVINR
                     TTFGFEINSVGLNPHASKYAGMRTKLLIV"
BASE COUNT          868 a          808 c          855 g         1007 t
ORIGIN      
        1 cgacccgacc tgcttgaaaa agcaaccctg acagacaagg acaaaaagtt cctggccagc
       61 tataaaaagt cgcttgaaga gtacaaagaa caaaagccag catcttaagt ctggcttttg
      121 tgtttaagat tatagaacat ataaaaggag aaataatgag tcattcaatg gaaaaacaag
      181 accgtattca atggggaaaa ctgtttttaa tcggctttgg cttttttggg gttagcgtgc
      241 tttggagcct gtacaatacc tacgtgccat tgcttttaga tgccaagttt ggccttgatt
      301 ccgtggcaat ttcaatcatt atggtactgg ataacatcgc ggcttttctt attcagcccg
      361 cggttggggt gtggtcagac aatttacgca ccaaaatagg gcgtcgtttg ccattcatta
      421 tcgttggcgc gccactcgcc gcggtagcct ttgggcttat ccccctggcg caaacattac
      481 cagtactcct ggcttgtacg gtaacaacac tgctggcgat ggcgttttgg cgcacgcccg
      541 tggtggcttt gatgccggac gttaccccca gcaagttccg ctcgcaagcc aacggcgtga
      601 tcaactttat gggtggtttg ggattggttt tggggacttc cgttggcgcg gccctggtaa
      661 agatggacca tcccttagct ggcgcgttcc cgttctggtt tggctccatt ctggttattc
      721 ttgcggcggt gctggtcttc atctttgtgc gcgaaccaga atatgaaaaa cacgaaggcg
      781 aagaaaaacc aaaaagtgcc agcacactgg atggcttgaa agaagtcttt accggcaaac
      841 aacgcgacgc gattctgatc tttttagcaa ttttcttttg gtttatcgcc tataacgcga
      901 ttgaaacttt cctgtcgtta tatggaagaa acttccttgg gctcagcgat agtggcgccg
      961 ggcaactgat taccagcgtg ggcgcgagct tcctgctgtt tgccatcccc gccggcttaa
     1021 ttggcgctaa atttggcaga cgcagaacta tcttgaccgg tctcgcgatt atggttttga
     1081 cgatgagttc aatgttcttt attcccaagg atattttact caccacgcat ggtcgcattc
     1141 cgatcctggg cgactttatg aacgtgacca tcatcctgct tttggcaggt attggctggg
     1201 ctctgattaa catcaactcc ttgccgatgg tggttgattt aacgaccaaa gaccgtgtgg
     1261 gtacctatac gggactctac tacttcttct cgacgctttc agccatcatc ggacccctgc
     1321 tgaacggttt gattgtaaaa ctttccggtg gcgattatgg tcagattatg ctggtggggc
     1381 cgatcttcat ggcgctggct tttctcgcga ttgtgtttgt gaaacgcggt gagattaatc
     1441 ctgaagctga ccgacgccat gccaaagact atacagaaga gtacgaaggt attgacgcga
     1501 ttttgtagcg tcttttctaa taactgaatt gtaaattttt gcaaatcccg caaaagaaag
     1561 caaggaattt cattgcggga tttgctttgt taatatatac ttaacacacg cataacggta
     1621 ccatgggtac caactttacc aaaatttaat ataaaaggag tatggaatga agaaacgatt
     1681 attatcaatt ttggctatct tgctcgttgc cggtttagtg ctcggtgcat gtacgccaaa
     1741 aacccccgta gtggatgaag cccccgaagg cgacgttgtt gatcaaccaa ccgaaacctt
     1801 taaagtcggc ctggttaccg acgttggtgt ggttgatgac aaaaacttca accaggcaac
     1861 atgggaaggt gttctcgccg cgcaaaaaga actcggtttt gaagccaagt acatcgagac
     1921 aaaagacgcc aaagactatg atgccaacat ccaacttttc gttgatgaag gttttgatgt
     1981 catcgtgacc gttggttttg cccttggcga agcaactatc aatgccgcaa aagccaatcc
     2041 ggaaattgcc ttcattggtg ttgaccaatt ccagggcgaa gtcgttcctg gtgtcgcagg
     2101 cttgatcttt gacgaagcgc tttctggtgt tttggctggt gttttggctg ctaatctcac
     2161 cgaaaccaaa accatcgcag ctgttttagg tactgacctg gttccgccag tggttaacta
     2221 caaccgcggt tatgttggtg gtgctaaatg gtttgatcct gaaatcagca ttatttccac
     2281 ctaccatccc ggtggcatgg atgtagcgtt tactgacccc gagtggggtg catcaaccgc
     2341 taaacaagct attgaacaag gcgctgatat tgtcttcgct gctgctggta ataccggtaa
     2401 tggcgcgttg attgaaaccg ctcagcatga tggtctgttc tgcatcggtg ttgacactga
     2461 ccagtggctg accgttcctg aagctcacaa atgtttggtt tcatccgcga cgaaagacat
     2521 cactttgtct gtggttgaac tcatcaagct tgcttatgaa ggcaacttcc cttcaggcaa
     2581 ctacgttggt ggttcctcac tggcaccttt ccacgatttt gaaagcatca ttacggaaga
     2641 aatccaagcc aaactcaaag aggccttaga agcctacaat gctggcaaga ttgaacttcc
     2701 tgaaaagtaa gtttagttac cagctgtaaa caacagcaaa ataaggtgcc actttcgtgg
     2761 caccttattc ctataaaaaa gattggaggt aatccagcgt ggaagaacaa acaccctccc
     2821 aaaataactg gttaaaaaaa gtttggtttt ccgtaagaat tccattttta gcaattatct
     2881 tagcgctttt agtgggcgcg attatcttat tagtttcagg tgcgaatcca ctggaagcct
     2941 attggcagct ttttaaaggg gctttcggct catggagaaa tatccaaaga gtcctggaaa
     3001 aatctacccc cttaatcttt gctggtttag ccgtggcatt aggttttaag gcgggtcttt
     3061 ttaatatcgg cgcgcaaggg caactcctgt tgggcgcgct tatggccgga tatgttggtt
     3121 ttgcagtcaa aggtttgcct gcttatattc acatccctct ggcccttttg gcgggattac
     3181 tgaccggcgc tgtgtttgcc gcgattccag gtttgttacg ggcttattcc ggcgcgcatg
     3241 aggtgatttc gaccatcatg ctcaactacg tggcaatcaa ccttagcgat tatttttctg
     3301 aaggtccatg gaaagacacc actccgggca acattgtttc gcgattgcca gctatccaac
     3361 catcagcaga gataccaaac attgggcctg tacccgtcgg ctttatcgtg gcggtactaa
     3421 cgtccttgtt gatttggttt gtgattaatc gcacgacttt tggctttgaa atcaactcgg
     3481 ttggattgaa cccacatgct tcaaagtatg ccggtatgcg cacgaaactg cttattgt
//
LOCUS       DAOSFC010000143         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24549_length_3535_cov_5.818966, whole genome shotgun sequence.
ACCESSION   DAOSFC010000143 DAOSFC010000000
VERSION     DAOSFC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24549_length_3535_cov_5.818966"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..1209)
                     /locus_tag="PK791_04460"
     CDS             complement(280..1209)
                     /locus_tag="PK791_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92598.1"
                     /translation="MTANLRNLNALDFSYLIRQSHTFENLDTLHLVTNGEPLSFMGMA
                     STLFAQKHTGIVLELDGKPLVVARAVQWQDEDFAQLQFINAMEPAESNNVAVLEALLK
                     EIGAWGLTRVMGDISTDSIYLSDFRKANFIVWASHRSYSLSAPVLGAPESSAWRTWTA
                     KDYANMKTLYQSLVPSRIRQYEPMTRSKVLGKVISDASGNLTGYVDLDYGSKGLWAQL
                     FLLPEASRPEVLEDLAGELYMEYGRPITFSARSYMPWLRLALDKLDAQASGERALIVR
                     HLAIKDAKPEQAGETIFEKSPAEGGIFSAESQN"
     gene            complement(1292..2179)
                     /gene="pdxS"
                     /locus_tag="PK791_04465"
     CDS             complement(1292..2179)
                     /gene="pdxS"
                     /locus_tag="PK791_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOH92599.1"
                     /translation="MEKSVSSFDKKKSVAESFVGGVIMDVVNADQAKIAEDAGAVAVM
                     ALERVPADIRVAGGIARMSDPELIIKIMEAVDIPVMAKCRIGHIAEAQILESLGVDFI
                     DESEVLTPADEQYHINKHVFNTPFVCGCRNLGEALRRIGEGAAMIRTKGEPGTGDVVE
                     AVRHARSVYGDIRHLLSLPDDEVMTFAKNIQAPYELCMEVRRLGRLPVVNFAAGGIAT
                     PADASLMMQLGVEGVFVGSGIFKSGDPLKRAKAIVEATTHYKDPEVLARVSRNLGEAM
                     VGRSVGELADNERMSERGW"
     gene            2438..>3535
                     /locus_tag="PK791_04470"
     CDS             2438..>3535
                     /locus_tag="PK791_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOH92600.1"
                     /translation="MTEKTYTQSPWSLKELFDSFDDPKMNEAYIKLEKMVSQFEAHRE
                     CLKPDIEADTFLALIKMQEDFTTLAYRVYGFASLSFAANTQDQKAQIAMARFQQFAAE
                     MGNRTLFFSLWWKALDEENANRLLDASGDYRYWLVQMRNFKPYTLSEAEEKIINIKNI
                     TGTNALEMLYESITNRYTFKLEVDGEEKELTRGELMSHVRSADPDLRARAYQELYRVY
                     GEDAPILGQIYQNIARDWKNENVSLRNFASPITVRNTINDLPDDVIDTLMDVSRKNIG
                     IFHRFFRLKAQKIGMEKLRRYDIYAPVSESDKRFEFNEAAALTFESFNRFDPKFAELA
                     QKVFDQEHVDSEVRKGKMGGAFCSTLGPELTP"
BASE COUNT          944 a          894 c          804 g          893 t
ORIGIN      
        1 ataacgatca tataattctg caaacgaact ggaatcgtta actgccaaac aagcaagttc
       61 gttatcatcc aattcagaca cccttatttg ttgactcatt gattttttcc aaaaaacttg
      121 ttccattttt ctctctcgtt aataagacgt ggaacttaca gtttcgtgcc gttttataaa
      181 aatgttttct acagcgtgtt tttacggtta catgaaaatc tcagacaaat ttctttttgt
      241 aagatattca cacatacgtg tgcgaatatc ttatacatat taattttgcg attcggcaga
      301 gaagatacca ccttcggcgg gagatttttc aaagatggtt tcgcctgctt gctcaggttt
      361 tgcgtctttg atggccagat gccggacgat gagcgcgcgt tcgccactgg cttgcgcgtc
      421 gagtttgtcc agtgccaaac gaagccaggg catataagat cgcgcgctga aggttatcgg
      481 gcgtccatat tccatataga gttccccagc caggtcttcc aaaacctcgg ggcgggatgc
      541 ttcgggcaaa agaaatagct gcgcccaaag gcctttgctg ccataatcaa ggtccacata
      601 acccgttaga ttgccagacg catcagagat aaccttaccc aaaacttttg accgcgtcat
      661 cggttcatac tggcgtattc gcgaaggcac caagctttgg taaagcgttt tcatgttcgc
      721 ataatctttt gccgtccagg tgcgccatgc ggaactttcc ggcgcgccca gaacaggcgc
      781 agataatgaa taagaacggt gactcgccca aacgataaaa ttagccttac gaaaatcgct
      841 aaggtagatt gaatccgttg aaatatcgcc catgacgcgt gtgagtcccc atgcgccaat
      901 ctctttgagc aaggcttcaa gcacagccac attgttgctt tcggcaggtt ccattgcgtt
      961 tataaactga agttgcgcga agtcttcatc ttgccactga acagcgcgcg ctacaaccaa
     1021 aggtttgcca tccagctcca gcacgatgcc agtatgcttt tgcgcgaaga gcgtgctggc
     1081 catgcccata aaactgagtg gctcgccatt cgtcaccaga tgtagcgtat cgagattttc
     1141 aaaggtgtga ctttgtctta tcagatagct aaaatccagg gcgttaagat tgcgtaaatt
     1201 tgcggtcatc gtactctaat tataaaagaa aatcagccct gtgagatagg gctgattaaa
     1261 taaactaagt gggcacctga atgatggcaa actaccagcc acgttcagac atacgttcat
     1321 tgtcggccag ttcgccaacc gagcggccaa ccatggcttc gcccaggttt cggctaaccc
     1381 gtgccagcac ttcggggtcc ttatagtgcg ttgtagcttc gacgatggct ttggcgcgtt
     1441 tgagtggatc gccagatttg aaaatgcccg aaccaacaaa aacgccttca acgcccagtt
     1501 gcatcatcag ggaagcgtcc gcgggcgtgg caataccgcc agcggcaaaa ttgaccacag
     1561 gcaagcgacc caggcgacgc acttccatgc acagttcgta aggtgcctgg atattcttgg
     1621 caaaggtcat cacttcgtca tcaggcaggg aaagcagatg gcggatatcg ccatacacac
     1681 tgcgcgcgtg gcgcacggct tcaaccacat cacccgtacc gggttcgccc ttggtgcgaa
     1741 tcatcgcggc gccttcgcca atgcggcgca gagcctcgcc taagttgcga cagccacaaa
     1801 caaaaggcgt gttaaaaacg tgtttgttga tgtgatattg ttcatcagca ggggtgagca
     1861 cttcggattc atcaataaaa tcaacaccaa ggctttcgag aatctgcgct tcggcgatgt
     1921 gtccaatgcg acacttcgcc atgacgggga tatcaactgc ctccatgatt ttaataatca
     1981 gttccgggtc gctcatacgc gcgatgccac cagccacgcg aatatccgct ggcacgcgtt
     2041 ccaatgccat aaccgccacg gcgccggcat cttcagcgat tttggcctgg tcggcattga
     2101 ccacgtccat aatcacgcca ccaacaaagc tttcagcaac gcttttcttt ttatcaaaac
     2161 tggaaacaga cttttccatt agcaacctcc aaattattac ttctttcatt tcgattgtac
     2221 cacttaagaa ggctaaaatc cttagagaga cattaataat aggctttgca gaatgtggct
     2281 tggaaattct tgcttgctgg gaacaaaaat attcacccaa gttttctggt aaagaaaata
     2341 aacctggctc cttgccacga aaagggtttt ttcttataaa agttatgcac tctgatgtat
     2401 acttttacta gattaattaa caaactggaa ggatgttatg accgaaaaga cctataccca
     2461 atccccatgg agcttgaaag agctctttga tagtttcgat gacccaaaaa tgaacgaggc
     2521 ttatattaag ctcgaaaaga tggttagcca atttgaagcc catcgtgaat gccttaagcc
     2581 cgatattgag gctgacactt tcctggcact gattaaaatg caggaagatt tcaccacact
     2641 cgcttatcgt gtgtacggtt tcgccagcct ttctttcgcg gccaacactc aggaccaaaa
     2701 agcgcaaatc gcgatggcac gtttccaaca gtttgctgcc gagatgggca accgcacgct
     2761 cttctttagc ttgtggtgga aggcgcttga tgaggaaaat gctaatcgtc tcttagacgc
     2821 cagtggcgat tatcgttatt ggctggtgca gatgcgcaac ttcaagcctt ataccctcag
     2881 cgaagcggaa gaaaaaatca tcaacattaa gaacatcacc ggtaccaacg cgcttgagat
     2941 gctctacgaa tccattacca atcgctacac ttttaaactc gaagttgatg gtgaagaaaa
     3001 agaactgacc cgtggtgagt tgatgagcca cgtgcgctcc gcggaccctg acctgcgcgc
     3061 ccgagcttat caggaacttt accgtgtgta tggcgaagat gcccctattt tgggccagat
     3121 ttaccaaaac atcgcccgcg actggaaaaa cgaaaacgta agcctgcgaa actttgccag
     3181 cccgattacg gtgcgcaata ctattaatga cctgcccgat gatgttattg acacgctgat
     3241 ggacgtttcc cgcaagaaca tcggtatttt ccatcgcttt ttccgtctca aagcgcaaaa
     3301 aatcggcatg gaaaaactgc gccgttacga tatctacgcg ccagtttccg aatcagacaa
     3361 gcgtttcgag ttcaacgaag ccgccgcatt gacttttgaa tccttcaatc gctttgatcc
     3421 caaatttgcg gaactcgcgc aaaaggtttt cgaccaggaa catgttgaca gtgaagtgcg
     3481 caaaggcaaa atgggtggcg cgttctgctc aaccctgggt cccgaactca cccct
//
LOCUS       DAOSFC010000144         3530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24615_length_3530_cov_4.019856, whole genome shotgun sequence.
ACCESSION   DAOSFC010000144 DAOSFC010000000
VERSION     DAOSFC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24615_length_3530_cov_4.019856"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PK791_04475"
     CDS             <1..520
                     /locus_tag="PK791_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOH92601.1"
                     /translation="DYYMMVHFDGFKAVVDSLDGIDVEITQTLSDSCHMDPSGWCTFE
                     PGVMHMDGQTALWYSRSRMTSSDFDRNRRTQDVIVAMAKKAMSRNMIIKIPELYGHYR
                     EYIDTDMPLSAVMKLMPMASKLKDFSNLRHFAVDSTMAYDWITWEGSMVLMPDNNAIA
                     RMLNEALYRNEQ"
     gene            658..1575
                     /locus_tag="PK791_04480"
     CDS             658..1575
                     /locus_tag="PK791_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOH92602.1"
                     /translation="MSQNSEPISAHIQETTLLHPAIQAWKIYLRDQGRSHHTLKAFSA
                     DLNLLESYLPTDKQIGQITTADLENHLYWMENERDVPCSPKTLSRRITSIKSFFRWLF
                     ESGVLKSNPADPIIQKPVASRLPHALTQSEYRLALEAARAIRDGAKADPRPLVLFQLV
                     LETALKKGECLSLMVEHLEIDDPKNAALHVRYSNPRYRYKERRLPLSQDWVEAYTDYA
                     QQYDIKDQVFPWSQRRLEYLLSDIAEEAKFEKNISFDSLRWTSAVIDLSKGVDPDSIR
                     QKLGISKIQWRDVQERLHKIAEEYDYTLS"
     gene            1611..2576
                     /locus_tag="PK791_04485"
     CDS             1611..2576
                     /locus_tag="PK791_04485"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOH92603.1"
                     /translation="MKMQSNKKRGCAFSTFLIVLVLLILGVMVGVSLNKIREGQPGFL
                     TNLWITPDKYLDDPYLSNEATRINVPNHVKTILLLGSDYQPEIGFRTDVMLLMAFNTK
                     TNKVNLISFPRDLWVTIPGLGEQRLNVAFPFGDWQLLSDTFALNFGFRPDHYMMADFE
                     GFKHILDVLGPIDVEVSERMEDASYLDASGWRVVEPGTVTMTQPEVFWYVRARMNSSD
                     IDRNRRAMEVIKAMGKKALKPAHLRYLPGNLRAVKRSIETDMELADFIIYALPLSKYF
                     DENVFTMYRIEYTNVTPGVTEGGASVLFPKFEEIKGILREVLDAN"
     gene            complement(2848..>3530)
                     /gene="tilS"
                     /locus_tag="PK791_04490"
     CDS             complement(2848..>3530)
                     /gene="tilS"
                     /locus_tag="PK791_04490"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOH92604.1"
                     /translation="QSENEIVLNSVAFLPLDLALQRRVVRRGLNALRPVNPDFGFEDI
                     EKTRQVIINGLGAQDLSQKTRAVMVGNACHLLEQSALPTILSMPQLDSDSTLELGVGE
                     SVFLNPGWQLSAEMISKQDYAKISDAEKKDPQQAWLNPADMALPLIVRAMQTGERWVP
                     LGLECGSQKLSDFFVNNKIPQAARAKWPVVLSGESVVWVVGLRIAHDWRLQGDENEIL
                     HLSLKHND"
BASE COUNT          900 a          952 c          826 g          852 t
ORIGIN      
        1 agattattac atgatggtcc attttgatgg ttttaaagcc gtggtggaca gtttggatgg
       61 cattgacgtt gaaattaccc aaacgctcag cgattcttgc cacatggacc ccagtggctg
      121 gtgtacattt gagcctggcg tcatgcacat ggacggtcaa accgcgcttt ggtactcgcg
      181 ttcgcgtatg accagcagcg actttgatcg caatcgccgc acacaagacg tgatagtggc
      241 tatggcaaaa aaggcgatga gtcgcaacat gattatcaaa atccccgaac tttacggtca
      301 ttatcgcgaa tatattgaca ccgatatgcc cttgagtgcc gtgatgaagc tgatgcccat
      361 ggcgtcaaaa ctcaaggatt ttagcaattt gcgacatttc gcggttgaca gcacaatggc
      421 gtatgactgg attacctggg aaggttcgat ggtgctcatg cccgataaca acgccatcgc
      481 cagaatgctc aacgaagccc tttatcgcaa cgaacagtaa gccacacgtt ttttatagaa
      541 tccccttccg gtaattgctt tgcccggcaa tgtacacgtg tcttgatttg acagacccac
      601 acattagggc taaacttaag gacagaactt atagctatac gaaattatgg aggcaaaatg
      661 agccaaaatt ctgaacccat ctctgcccat atccaagaga cgactctctt gcacccagcc
      721 attcaggcct ggaaaattta cctgcgcgac cagggcagat cgcaccatac ccttaaggcc
      781 tttagcgcgg atctcaatct gcttgaaagc tacctgccca ccgacaaaca aatcgggcaa
      841 attacaaccg ccgacctgga aaaccacttg tactggatgg aaaatgaacg cgatgtcccc
      901 tgtagcccca aaacgctctc caggcgcatt acatcaatca agtccttttt tcgctggttg
      961 tttgaatcgg gcgtgctcaa aagcaacccc gccgacccca tcattcaaaa gcctgtcgcc
     1021 agccgattgc cccacgcgct gacccaatcg gaataccgcc tggcgcttga agccgcgcgg
     1081 gccattcgcg atggtgccaa agccgacccg cgacccctgg tactctttca gctggtgctc
     1141 gaaaccgcgc tcaaaaaagg cgagtgcctg agtttgatgg ttgaacatct cgaaattgac
     1201 gaccccaaaa acgccgcgct ccatgtgcgc tattccaacc cccgctatcg ctacaaagaa
     1261 cgacgcttgc ccctttctca ggactgggta gaggcctaca ccgactacgc ccagcaatac
     1321 gacatcaaag accaggtctt cccgtggtcg caacgtcgtt tggaatatct gctaagcgat
     1381 atcgccgaag aagccaaatt cgagaagaac atttcctttg acagtctgcg ctggaccagc
     1441 gccgtcattg accttagcaa gggcgttgac cccgacagca ttcgccagaa actgggtatt
     1501 tccaaaatcc agtggcgcga cgtgcaagaa aggctgcata aaatcgccga agaatacgat
     1561 tacacgcttt cttaggcatg ggctaaacgc caaacaataa aggtgtttct atgaaaatgc
     1621 aatcaaacaa aaaacgcggt tgcgcgtttt caacttttct tatagtcctg gtactcttga
     1681 tactgggcgt gatggttggc gtgagtctta ataaaattcg cgaaggccag cctggttttt
     1741 tgaccaacct gtggattaca cccgataaat atctcgatga cccttatctt tccaatgaag
     1801 ccacgcgcat caacgtgcct aatcacgtca aaacgattct gctgctcggc tcggactatc
     1861 agcccgaaat cggctttcgt accgacgtga tgctgctgat ggccttcaac accaagacca
     1921 acaaggtcaa tctcatctct ttcccgcgcg acctttgggt taccattccc ggactgggcg
     1981 aacaacgttt aaacgtcgcc ttccccttcg gcgactggca actgctgagt gataccttcg
     2041 ccctgaattt cggttttcgg cccgaccatt acatgatggc tgatttcgaa ggttttaagc
     2101 atatcctgga tgtgctcggt cccatcgatg ttgaagttag cgaacgcatg gaagacgcaa
     2161 gctatcttga tgccagcggg tggcgcgtgg tagaacccgg cacggtaacc atgacccagc
     2221 cggaagtgtt ttggtacgtg cgcgcgcgca tgaactcgtc cgatatcgac cgcaaccgcc
     2281 gcgcgatgga agttattaaa gccatgggca aaaaggcgct caagcccgcg catctcaggt
     2341 atctgcctgg caacttgcgc gcggtcaaac gcagcattga aaccgacatg gaacttgccg
     2401 atttcatcat ctacgccctg cctttgtcta agtacttcga tgagaacgtc tttaccatgt
     2461 atcgcattga atacaccaac gtcacgcctg gcgttaccga aggtggcgcg tcggttttat
     2521 tcccgaaatt tgaagagata aagggtattt tgcgtgaggt cttagacgcg aactaaactg
     2581 gcgtcatagc ttattgtatc ttattcaccc cctcctggga gggggattaa agggggtggg
     2641 tttttttcgt gaatattctt ggaaaaacta caaacagttt gccattaatt tgtcattaag
     2701 tgaactttat agattgaaat tttactagta ataaagccca cacccctccc cctctcccag
     2761 gataggggga attttaatgc gcgcccaggc aattaatgcc acagaaaact gtattgtctt
     2821 agagaaaatt gttctacaaa actggcttta gtcgttatgc tttaaagata aatgtaaaat
     2881 ctcgttctcg tcgccttgca agcgccagtc atgcgcgata cgcaagccaa cgacccaaac
     2941 aaccgattcc ccactcagca ccacaggcca tttggcacgg gctgcctggg gaattttatt
     3001 attgacgaag aaatcgctca gtttctgact gccacactcc agtcccagcg gcacccagcg
     3061 ctcgcccgtt tgcatcgcgc gtacaatcaa gggcaatgcc atgtcagcag gattgagcca
     3121 ggcttgttga gggtctttct tttccgcgtc tgagattttt gcgtagtcct gtttgctaat
     3181 catttcggcg ctgagttgcc agccagggtt gagaaaaaca ctctcgccaa cgccaagctc
     3241 aagcgtgctg tcagaatcaa gttgtggcat gctcaatatc gtcggcaaag cgctttgctc
     3301 aagcagatga caggcattcc caaccatcac cgcccgcgtt ttctggctca agtcttgcgc
     3361 gccaagcccg ttgatgatga cttggcgtgt tttttcgatg tcttcaaagc caaaatcagg
     3421 attaaccggg cgcaaagcgt tcagcccgcg ccgaacgaca cgtcgctgca aagccaggtc
     3481 aagcggcaaa aaggctacgc tgtttagaac aatctcattt tcgctttgcc
//
LOCUS       DAOSFC010000145         3524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24683_length_3524_cov_5.269530, whole genome shotgun sequence.
ACCESSION   DAOSFC010000145 DAOSFC010000000
VERSION     DAOSFC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24683_length_3524_cov_5.269530"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PK791_04495"
     CDS             complement(<1..259)
                     /locus_tag="PK791_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOH92605.1"
                     /translation="MQKAELNELYDEWIQAIYRYTLSRVKHVDDAQDITSQTFLSAWQ
                     NRERYQERGNFAAWLFSIARNKINDHFRQAQRKQNRIQTLGD"
     gene            complement(635..1399)
                     /locus_tag="PK791_04500"
     CDS             complement(635..1399)
                     /locus_tag="PK791_04500"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH92606.1"
                     /translation="MKNKPGIVGVLIAVFVLFLLVSPALANGEAYSLVFRREFGVGMP
                     GAVQGRMSMTIKGDLDAVDKVVYMIDGQEMANMTAPSLKFQFNTDDYPSGQHTIYALV
                     TTKTGETKQTDSISVTFMAADEANVIIRNVFIGVGAVLLVTVIVSVLVTKRQLDASKS
                     DGVSAVGLWGAAVCPKCGRAFTRTVIGLNLLGKRYEPCPHCRKWSMTNRATKEEIEQA
                     EQKFKATIAEEISEPIRSDSQDDKLDLIEESKYTEL"
     gene            complement(1406..2221)
                     /locus_tag="PK791_04505"
     CDS             complement(1406..2221)
                     /locus_tag="PK791_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH92607.1"
                     /translation="MKNIRTIIKKEWAEVFKRRVVVLIMLLVPLLFTLIPLVMLGVMS
                     NTSVGVGADVSPEMLAQSGSLPGDMEAVCAGLSGNDCMQVMMVIQFLLMFMMMPLLLP
                     ITIAAYSIVGEKTTRSLEPLLATPITTLELLVGKCLAAVIPAVGATWASFALFNIGLI
                     LVKVSPGVRAYILGPTWLLGILVLGPILAIIAVNFALYVSSRVSDPRMAEQVSGVLIF
                     PFMGVILAQLAGVITINIKFMLLSIVVCALIAVGMFYLGTIIFDRENILTKWK"
     gene            complement(2218..3198)
                     /locus_tag="PK791_04510"
     CDS             complement(2218..3198)
                     /locus_tag="PK791_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92608.1"
                     /translation="MIKTQDLRKIFKIGKKQKETLVAVDGLNLKISEGEVFGFLGPNG
                     AGKTTTVRMLTSLIRPSSGSAEIYGLKVGVDDTEIRRNVGILTESPGLYERLSAEQNL
                     RIFAELYDIPDPEKAINKYLSMLDLWDRRMDAAGTYSKGMRQKLAVARAMLHEPKVLF
                     LDEPTTGLDPEAAKTVRDFIDELKHQGRTIFMCTHNLDEAERHCDRVGIFKQRLLALD
                     KPSALREQLFGRKVVFHLANATPEMCAVVQALPDVKALEQIENKLLLSLDEPEKLNPV
                     ITRALVDKGADILFIGEIKQSLERVYLEMIRSEESSQTKKINQSVERNRA"
     gene            complement(3312..>3524)
                     /locus_tag="PK791_04515"
     CDS             complement(3312..>3524)
                     /locus_tag="PK791_04515"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH92609.1"
                     /translation="LVTTTQHGTLILSADGSFMYVPDAGYSGEDTFTYKAFDGEYYSE
                     EVTVTLIVVKKPVWNLYIPILLTGGF"
BASE COUNT          994 a          857 c          713 g          960 t
ORIGIN      
        1 catcgcccag cgtttggatg cgattctgct ttcgttgggc ttgcctgaaa tgatcgttga
       61 ttttattgcg cgcgatgcta aagagccagg cggcgaaatt gccacgttcc tgatatcgtt
      121 cacgattttg ccaggcagag agaaaggttt ggctggtaat gtcctgggca tcgtccacat
      181 gctttactct tgagagcgta tagcgataaa tcgcttgaat ccattcgtcg taaagttcat
      241 tcagttcagc tttctgcata aacctccatc ttcgcgcgct ttgttaagga taacgtcatc
      301 caagtacttt ttgtgacagt tatatcaaat acctcttcaa aacaaagcaa cctttgacat
      361 ttaagcctat gaaaattatt ccataaaaga atagcatgtg taacaagcgt aagagagcaa
      421 actccactat tcagcatttt aaagtgcgta taagaacata tgttcgtttt cacagtcctg
      481 ttttctgaca acacctttcg cacaagcgga tatgtttcga gtttatctgg attattaatt
      541 acttttttac aacaaatgga taatgcaatt accttacgac tattctagat tcatcaataa
      601 atactactgc caagtttaaa aaagaactta ctgcttacaa ttctgtatat ttactctcct
      661 caattaaatc aagtttgtca tcctgcgaat ctgaccgaat aggttctgag atctcttccg
      721 ctatcgttgc cttgaatttt tgttcagctt gctcgatttc ctcttttgta gcacggttgg
      781 tcatggacca ttttcggcaa tggggacagg gttcgtagcg cttgccaagc aagttcaagc
      841 caatcaccgt tcgtgtaaat gcgcgtccgc actttgggca gacagctgct ccccacaaac
      901 caacagcgct tacgccatcg ctttttgatg catcaagctg acgttttgtt acgagaacac
      961 tcactattac agttaccaat aaaaccgcac caacaccaat aaaaacattt cggataataa
     1021 cgttagcttc atcagccgcc atgaatgtta cggaaatgga atcggtttgt ttggtttcac
     1081 cagttttggt cgtaacaagc gcataaatag tatgttgacc agacggataa tcatctgtgt
     1141 tgaactgaaa cttgagtgat ggcgcggtca tgtttgccat ttcttgccca tcaatcatat
     1201 agactacttt atcaacagcg tccaaatcgc ctttgatggt catactcatg cgcccttgca
     1261 ccgcgccagg cataccaacc ccaaattcgc gcctgaacac cagactataa gcctcaccgt
     1321 tcgccaaagc aggactaacg agcaagaaca aaacaaaaac agcgatcaag acgccaacaa
     1381 tacctggctt atttttcatc caaccctact tccatttagt aagaatattt tctctgtcaa
     1441 aaataattgt tcccaaataa aacataccca ccgcaataag cgcgcaaact acaattgata
     1501 agagcataaa cttgatatta atggtaatca cgcctgccaa ttgcgccagg attacaccca
     1561 taaaaggaaa aatgagtaca ccagacacct gttcagccat acgtggatct gaaacgcgtg
     1621 atgaaacgta aagcgcgaaa ttcaccgcga taattgccaa aatgggaccc agcaccaaaa
     1681 tgcctaaaag ccaggttgga cctaaaatat aagccctaac tccgggactg acttttacga
     1741 gtatcaaacc tatattaaac aaagcaaacg atgcccaggt ggcaccaacc gcgggaatga
     1801 ccgcggcgag gcatttcccc accaggagtt ccagcgtggt gatgggcgtt gccaaaaggg
     1861 gttccaaact gcgcgttgtc ttctcgccaa cgatactgta agcggcaatg gtaatgggaa
     1921 gaagcaaagg catcatcatg aacataagca aaaactgaat caccatcatg acttgcatac
     1981 aatcattacc cgacaagccc gcgcaaacgg cttccatatc ccctggcaac gagccagact
     2041 gtgccagcat ttcaggactt acatcagcgc caacgcccac actggtatta gacatcacgc
     2101 ccaacatcac cagcggaata agtgtgaaca acaaaggcac aaggagcatg attaaaacaa
     2161 cgacccgtcg cttgaagact tctgcccatt cttttttgat aatggttcga atgtttttca
     2221 tgcccggttc ctttctacgc tttggttaat cttttttgtc tggctggatt cttcactgcg
     2281 gatcatttcc agatagacgc gttccaaaga ttgcttgatt tcaccaataa acaagatatc
     2341 cgctccttta tccactaaag cgcgtgtaat aacaggattc aatttctctg gctcgtccaa
     2401 actgagcaac aatttgttct caatttgttc caaagcttta acatcaggca aggcttgaac
     2461 cacagcgcac atttcgggcg tcgcgttggc caaatggaag actactttac gcccaaaaag
     2521 ctgttcgcgt agcgcggacg gtttgtccaa cgctaaaaga cgttgcttga aaatgcccac
     2581 gcggtcgcaa tggcgttcag cttcatcaag gttgtgggta cacataaaga tagtgcgccc
     2641 ctgatgtttg agttcatcaa tgaaatcacg cacagtttta gcagcttcgg ggtccagtcc
     2701 agtggtgggt tcatcaagga aaagcacttt tggctcatgg agcattgccc gcgcaaccgc
     2761 cagcttttgg cgcatacctt tggagtaagt tcctgcggcg tccatgcgcc tgtcccacaa
     2821 atccagcata cttaggtatt tgttgatggc tttttcggga tccggaatgt cataaagttc
     2881 agcaaaaatg cgcaggtttt gttccgcgct aagacgttca tataagcctg gcgattcggt
     2941 caaaatgccc acgttgcgtc gaatctcagt atcatctacg ccaactttca atccgtaaat
     3001 ttctgctgaa ccggagctag ggcggattag actggtaagc atgcgcaccg ttgttgtttt
     3061 gcccgctccg tttggcccca gaaagccaaa cacttcacct tccgatattt tcaagttcaa
     3121 cccatcgaca gcgaccaggg tttctttttg cttctttcca attttgaata tttttctcag
     3181 gtcttgtgtt ttaatcatag gctcatccaa acgtactatt ctgtgtaatt gtaacgtatt
     3241 aaaaactaag gctggcaaat cttagtgttt gccagcctca tagctagtta tggttcagaa
     3301 gtcatgttaa actagaagcc acctgtcagc aatatgggga tgtataaatt ccaaactggt
     3361 ttcttgacaa caatcaaggt aacggtgact tcctcactgt aatattcacc atcaaaggct
     3421 ttgtaggtga aagtatcttc gccagagtag cctgcatcag gtacatacat aaagctacca
     3481 tcagcactca agatgagcgt gccatgctgt gtggtcgtaa ccag
//
LOCUS       DAOSFC010000146         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_24852_length_3507_cov_7.624855, whole genome shotgun sequence.
ACCESSION   DAOSFC010000146 DAOSFC010000000
VERSION     DAOSFC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24852_length_3507_cov_7.624855"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PK791_04520"
     CDS             <1..1043
                     /locus_tag="PK791_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOH92610.1"
                     /translation="GLLNTWRTEMSEKLSILIPMAGLGSRLRPLTWSRPKPLLSLAGA
                     TVLDHLLAMFKTIPNQDEIEYVFIMSPGQEPVIKSYMKNHHPEKNINYVEQRTMRGQS
                     DALWQARSFMDGPTLTIFSDTLIDSDFSNLENETADAIAWVKPVPDPRRFGVVEVDAS
                     GYVTQLIEKPNEMHNNLAVVGCYYFKEGKDLTKAIKEQIARNISLKGEYFLVDALNIL
                     LEEGSKMRIEQVSTWLDAGTSEALLDTNRHLLENGRDNMKDIHTLADSVIRPPVFIHP
                     DARVSGSIIGPFTSIGANCVVQNSSISDSILSPGAHVSNSHLDASLIGYDCVINSLSG
                     KFNLGDNSWASS"
     gene            1078..2235
                     /locus_tag="PK791_04525"
     CDS             1078..2235
                     /locus_tag="PK791_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH92611.1"
                     /translation="MIDFINPRVTGLKPSGIRKYFDIALTMDDVISLGLGEPDFTTPQ
                     PIVEAGIRALKEGATHYTANLGLLELRQELGAHLNKLYKVNYAPDEIIITVGVSEALY
                     LTCTAILQPGDEVIIPTPAFVSYEPEVKLAGGVVVEVATYEENLFVPKIEDIAAAVTE
                     KTKAIVLTYPNNPTGAVASRETLLQIAELAQKHNFLVIADEVYDRLVYGQEHVCFSAL
                     PGMKDRTILLGGWSKSYAMTGWRLGYACAHREIISAMVRVHQFSVMCAPIMAQYAGLE
                     ALRVGEPYVLEMVAEYDRRRKLIVDGFNSLGMPTFEPKGAFYCFPRISGFGMSDEEFC
                     MGLLKEEGVVIVPGDSFGDAGIGFARASYATSYEQIEKALERIARYIKKFA"
     gene            2411..>3507
                     /locus_tag="PK791_04530"
     CDS             2411..>3507
                     /locus_tag="PK791_04530"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOH92612.1"
                     /translation="MTENTNAFTMAQAQFDKVADQLDLSQDIRDMLRWPLKQFTVRIP
                     VRMDDGTLRVFTGFRVQHNDVRGPNKGGIRFAAGETIDTVNALAMWMTWKTAVADIPL
                     GGGKGGVDVDPSTLSEGEKERLVRGYIRALWKELGPRIDVPAPDVGTNAQMMGWMMDE
                     YSAIVGQYTPGVITGKPLGGGGSEGRTEATGFGVCINIREAMKHLGINPKETKAALQG
                     FGNVGQYVAIGYPLYVGGKVICVSCWDREDRKSYTFTKEDGIDAMFLKSITDQYGTID
                     KEKAKAAGYLIEDGNAWLSKDVDVVVPAALEGQITGETVHQIHANVKMIAEAANGPTT
                     PEADKVIAERGIFLVPDFLCNAGGVTVSYMEG"
BASE COUNT          883 a          890 c          894 g          840 t
ORIGIN      
        1 ctggcttgct aaatacctgg aggacggaaa tgtcagaaaa actaagcatc ctgataccga
       61 tggctggctt aggctcacgc ttgcgcccct taacctggag cagacccaaa cctctgcttt
      121 cgctggctgg tgccaccgtg ctggaccatc ttttagcgat gttcaagacc attcccaacc
      181 aagatgagat tgagtatgtt tttattatgt ctcctggcca ggaaccggtt atcaaatctt
      241 acatgaagaa tcaccaccct gaaaaaaaca tcaactatgt tgaacaacgc accatgcgtg
      301 ggcaatctga tgccttgtgg caagcccgct cttttatgga tggccccaca ctgaccatct
      361 tctcggatac gctcatcgac agcgacttta gcaatcttga aaatgagact gctgacgcca
      421 tcgcctgggt gaagcccgtg ccggacccgc ggcgttttgg tgttgtggag gtggatgcgt
      481 ctggctatgt aacccaactc atcgaaaaac caaacgaaat gcacaacaat ctggcagtgg
      541 tcggttgtta ttactttaaa gaaggcaaag atttaaccaa agccatcaag gaacaaatcg
      601 cgcgcaatat atcgcttaag ggtgagtatt tcctggtgga cgcgctgaac attctgcttg
      661 aagaaggcag taaaatgcgc attgaacagg tgagtacctg gcttgatgcg ggtacatctg
      721 aagccttgct ggacaccaac cgccatcttt tggagaatgg tcgcgacaat atgaaagata
      781 tccacacact tgcggatagc gttatccgtc cgccggtttt tatccaccct gacgcgcggg
      841 tgagtggctc gattatcgga cccttcactt ccataggcgc aaactgtgtg gtgcaaaaca
      901 gctctatcag cgactcaatt cttagccccg gagcgcatgt gagcaattcg catctggacg
      961 cttcgcttat cggctatgac tgtgttatca attcattaag tggcaaattt aacctgggag
     1021 acaactcctg ggcaagctct taaacaaatc agaactaaat tcaacaaagg agatatgatg
     1081 attgatttca taaacccacg cgttactgga ctgaaacctt ccggcattcg gaaatatttc
     1141 gacatcgccc tgactatgga cgatgtcatc tcgcttggtt taggggagcc tgactttacc
     1201 acaccgcaac ccatcgttga ggctggcatc cgcgcgctta aagaaggcgc cacgcactac
     1261 accgcaaatt tgggactgct ggaactgcgc caggaactcg gcgcgcacct gaataaactt
     1321 tataaagtca actatgcgcc cgatgaaatc atcatcaccg tcggcgtgtc cgaggcgctg
     1381 tatctcacct gtacggctat cttgcagccc ggcgatgagg ttatcatccc aacaccggct
     1441 tttgtttcct acgaaccgga agttaaactg gctggaggcg tggttgttga ggtggcgacg
     1501 tatgaggaaa atctctttgt accgaaaatt gaagatatcg ctgccgcggt tactgaaaaa
     1561 accaaagcca tcgttcttac ctatcccaac aaccccaccg gtgctgtagc cagccgtgaa
     1621 accttgctgc aaatcgccga actcgcccaa aagcataatt tcctggtgat tgctgatgaa
     1681 gtttacgacc gcctggtcta cgggcaagag catgtttgtt tctccgcttt gcctggaatg
     1741 aaagaccgca cgattctttt gggcggttgg tcaaaatctt acgcgatgac gggctggcgt
     1801 ttgggttatg cctgcgcgca ccgtgaaatc atcagcgcga tggtgcgcgt gcatcagttt
     1861 tctgtcatgt gcgcgccgat tatggcgcaa tatgctggct tggaagccct gcgcgtaggc
     1921 gaaccttatg tgctggaaat ggtcgctgaa tacgaccgcc ggcgtaagct gattgtggat
     1981 gggtttaatt ctttgggtat gccaaccttt gaacccaaag gcgcgtttta ctgcttcccg
     2041 cgtatctcgg gctttggtat gagcgacgaa gaattttgca tgggactgct caaggaagaa
     2101 ggcgtggtga ttgtgcctgg cgatagcttt ggcgatgccg gcatcggttt cgcgcgggct
     2161 tcctacgcga cgtcttacga gcaaatcgaa aaagcgcttg aacgtatcgc gcgctatatc
     2221 aagaagttcg cataaacgag tgaaggagtt gcttaaaagc gcaatttgag atatggttaa
     2281 gatgctgaat atgcgaggag atatgggact gaggaccttc tcaaatgaat acatcaacac
     2341 aggctggtgc cctattttgc gtaccagtct ttcttttttc aaaacaaaac ccaacaaagg
     2401 agaataatta atgacagaaa atactaatgc ttttaccatg gcgcaagctc aattcgacaa
     2461 agtggctgat cagctcgatc tcagccaaga tatccgcgat atgttgcgct ggccactgaa
     2521 acaattcact gtccgcatcc ctgttcgcat ggatgacggt actttgcgtg tcttcaccgg
     2581 cttccgcgtg caacataacg acgtgcgtgg ccccaacaag ggcggcatcc gctttgctgc
     2641 tggcgaaacc atcgacactg tcaacgcttt ggcaatgtgg atgacctgga aaacagccgt
     2701 tgctgatatc cccctcggtg gtggtaaagg tggcgtggac gtagatcctt caacgctttc
     2761 agaaggcgaa aaagaacgtc tggtacgtgg ctacatccgc gcgctctgga aagaacttgg
     2821 tccccgcatc gacgtgcccg cgcccgacgt tggcaccaac gcccaaatga tgggctggat
     2881 gatggacgaa tattcagcca tcgttggtca atatactcct ggcgttatca ccggtaaacc
     2941 cctcggtggt ggcggctcag aaggccgcac cgaagcgacc ggttttggtg tttgtatcaa
     3001 cattcgcgaa gccatgaaac accttggcat caaccccaaa gaaaccaaag ccgcattgca
     3061 gggttttggt aatgttggtc aatatgtcgc catcggctat cccctttacg tgggtggaaa
     3121 agtgatctgc gtttcctgct gggaccgcga agaccgcaaa tcctacactt tcaccaaaga
     3181 agatggcatt gacgcgatgt tcctgaagag catcaccgac caatacggca ccatcgacaa
     3241 agaaaaagcc aaagcagccg gttatctgat tgaagatggc aacgcctggc tttccaagga
     3301 tgtggacgtg gttgttcctg ccgcgctcga aggccagatt acaggcgaaa ccgtgcatca
     3361 aattcacgct aacgtgaaga tgattgccga agccgccaac ggccccacaa ctcccgaagc
     3421 cgataaagtg attgcagaac gcggtatctt cctggtgcct gacttcctgt gcaacgctgg
     3481 tggtgtgact gtttcctaca tggaagg
//
LOCUS       DAOSFC010000147         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_25235_length_3469_cov_5.013181, whole genome shotgun sequence.
ACCESSION   DAOSFC010000147 DAOSFC010000000
VERSION     DAOSFC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25235_length_3469_cov_5.013181"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..952)
                     /locus_tag="PK791_04535"
     CDS             complement(143..952)
                     /locus_tag="PK791_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HOH92613.1"
                     /translation="MSYFAHNGNYPFGIDLSRYNATADLKRWPDFDLVAKHVPKVEFV
                     AIRAGVSWGYRDPMFKRFYKESTRIGAFVLPYHVLYPGEPALKQMNHFLNILEGIDLD
                     TVRLVLDVELVHDQSRSMITNTLLGCLDLLKYETGRYPIVYSRALWINEHVHIKDLPE
                     LDWWLAHYYARRPWPAYTPEYPCPPLMPKGVDSWLIHQTAERAPAIGGVGNFMDYNRW
                     NGTSESLLAYFGRGEPVRPALCPVDGLACPRQHILPATRYVLPLPAEAEAI"
     gene            complement(952..2643)
                     /locus_tag="PK791_04540"
     CDS             complement(952..2643)
                     /locus_tag="PK791_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92614.1"
                     /translation="MLNTAALGPMALMGFALPDLHTRRIQLNAEQARLAKTREEESLF
                     IQRLREIAIFSQYMGKTVLRSYQLEVAEAIIDSIVHQRGHSIVVMFPRQSGKNHVQAQ
                     IEVYLMALFAQRGADMIKFSPTYAPQCQNAMRRLEAALEANSFTKGRWRKSSGNHYRL
                     HNAHLTFLSAAPGSNIVGATAATMLALDEAQDIEIDKYDKQIAPMAASTNATRVFWGT
                     AWSGQTLLARELRQAQEAEARDGMRRVFRLSAEEVRQEVPAYGAFVDEQVRRLGRMHP
                     MVRSQFFSEEIDFAGGLFPASRLGLMHGTHEALESPAKGKQYALLIDLAGEDEAARSG
                     TFTPDSDLQNANRDATAITVVEVDLSKLDDALLAKPCYKVVRRYHWLGEKHSTLYARI
                     LNLVAHWNATRVVVDASGVGAGVSSFLREKLGDCVTALTFNRQIKSKLAWGFLSVIDT
                     GRFQDFKPKLRAEREDGVLNPLAMVMQDEQDSLQALFYKQLSAVSYEVSVGPERYIRW
                     GVTDGTRDAEGTLIHDDLVLSAAMVAVLDEQEWHLGYEPLLIQAVDPLLVIDGGY"
     gene            complement(2636..3088)
                     /locus_tag="PK791_04545"
     CDS             complement(2636..3088)
                     /locus_tag="PK791_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92615.1"
                     /translation="MEHTNPTLFARTQNSGHPCHSMAGVAAQETNDGLKDINYAERLQ
                     LSESARFGMDEEIAMLRKMIRNFTRAAGTSGEEDYPDNLAKSLDLLGLTCSRLASVLR
                     VNMMLAGSHEDDLRKQIEDSLAEFLAEVKTVKTQSKSKKRSQKKVKDA"
BASE COUNT          764 a          977 c          894 g          834 t
ORIGIN      
        1 ttttcactct cattaagcgc cagttgcgtt ctctcggcga gttctgtatc tggtgtatct
       61 gctttgctgt ctgtcgtggt ttcgcttgcg ttttcgttaa accacgccaa acggtcacgg
      121 atgcgttcca ggaaggatgc cattagattg cctccgcttc agccggcaag ggcaggacat
      181 agcgggtcgc cggcaagatg tgctggcgcg gacacgccaa accatccaca gggcaaaggg
      241 ctggcctgac cggttcaccc cgcccaaagt aggccaatag cgactcgctc gtgccgttcc
      301 agcggttgta atccataaaa ttccccacac cgccaatcgc tggcgcgcgt tccgcggtct
      361 gatgaataag ccagctgtca acgcctttgg gcattaaagg cgggcatgga tattccggcg
      421 tgtacgcggg ccagggtcgg cgcgcgtaat agtgcgccag ccaccagtcg agttcgggca
      481 aatccttgat atgcacatgc tcgttgatcc acaatgcccg cgagtagaca atcggataac
      541 gcccggtttc atatttcagc aaatccaaac agcccagcag ggtgttggta atcatcgaac
      601 gcgattgatc gtgcaccagt tccacatcca gtaccaggcg cactgtatcc aggtcgatgc
      661 cttccagaat attcaaaaag tggttcattt gcttcagggc tggctcgccg ggataaagca
      721 catgataggg caaaacaaag gcgccgatgc gcgtgctttc cttatagaag cgcttaaaca
      781 tcgggtcgcg atagccccag ctgacgcccg cgcgaatcgc cacaaactca actttgggca
      841 cgtgctttgc caccaggtca aaatccggcc agcgtttcaa atccgctgtg gcgttgtagc
      901 gcgaaaggtc aatcccaaaa gggtagttac cgttatgcgc gaaataactc atcaataacc
      961 tccatcgatg actaaaagcg gatccaccgc ttgtatcagg agtggctcgt agcccagatg
     1021 ccactcttgc tcgtccaaaa cggcgaccat cgccgctgac agcactaaat catcatgtat
     1081 caacgttcct tcggcgtcgc gggtgccatc ggtgacgccc cagcgaatat aacgctcggg
     1141 acccacgctc acctcgtagc tcaccgccga aagctgcttg taaaacaaag cttgcagact
     1201 gtcctgctcg tcctgcatca ccatcgccaa aggattaagt acgccatctt cacgctccgc
     1261 gcgcagttta ggcttgaagt cctgaaaacg ccccgtgtca atcaccgaca aaaagcccca
     1321 tgccagtttg gacttgattt gccggttgaa agtcagcgcg gtgacgcaat cccccagttt
     1381 ttcgcgcaaa aaggaactga cccccgcgcc cacaccgctg gcatccacca cgacccgcgt
     1441 ggcgttccag tgtgccacca ggtttaaaat gcgcgcgtag agcgtgctgt gtttttcgcc
     1501 cagccagtga tagcgccgca cgaccttgta acagggctta gccaatagag cgtcgtctag
     1561 cttgctcaaa tccacctcca ccacggtaat cgcggtggca tcacggttgg cattttgcag
     1621 gtcgctgtcg ggtgtaaacg tgcccgaacg cgcggcttca tcttcgcccg ccaggtcaat
     1681 cagtaaggcg tattgcttgc ccttcgctgg actttccagt gcctcgtgtg tgccgtgcat
     1741 caagccaaga cgcgaagctg ggaacaagcc acccgcgaag tcgatttcct cgctaaagaa
     1801 ctgactgcgc accatgggat gcattctgcc cagacggcgc acctgctcgt ccacaaaagc
     1861 gccataggcg ggcacttcct ggcgcacttc ttccgcgcta aggcgaaaca cgcgccgcat
     1921 gccatcgcgc gcttcggctt cctgagcctg gcgcagttca cgcgccaaca gggtttgtcc
     1981 gctccaggcg gtgccccaga aaacacgcgt ggcgttggtg gaagccgcca tcggggcgat
     2041 ttgcttgtcg tatttgtcaa tctcgatatc ctgggcttcg tccagtgcca gcatggttgc
     2101 agccgtcgcg cccacaatgt ttgaacccgg tgccgctgac agaaaagtga gatgcgcgtt
     2161 gtgcaggcgg taatggttgc cgctgctttt gcgccagcgt cctttggtga agctgttcgc
     2221 ctccagcgcg gcttcgagtc ggcgcatcgc gttttggcac tggggcgcgt aagttggcga
     2281 gaacttaatc atgtccgcgc cacgctgcgc gaaaagcgcc atgagataga cttcaatttg
     2341 cgcctgcacg tggtttttgc cactttggcg cgggaacatg accacaatcg aatgtccccg
     2401 ttgatgcaca atcgaatcga taatcgcctc agccacctca agctgataac tgcgcaaaac
     2461 cgttttaccc atatattgcg aaaagatggc tatctcgcgc agtctttgga taaaaagcga
     2521 ttcttcctcg cgtgttttgg cgaggcgcgc ttgttccgca tttagttgta tgcgcctggt
     2581 gtgtaagtcg ggcaaggcaa agcccatcag tgccatgggt cctaaggcag cggtattaag
     2641 catctttcac cttcttttgc gaacgttttt tagatttact ttgtgttttg actgttttga
     2701 cttccgccag aaactctgcc agactgtctt caatttgttt gcgcaagtca tcttcgtgcg
     2761 aacccgccag catcatgttc acgcgcaaaa cgcttgccaa acgcgaacag gtcaaaccca
     2821 agaggtctag cgattttgcc agattgtccg ggtagtcctc ttcgccactg gtgcccgccg
     2881 cgcgcgtgaa attgcgaatc atcttgcgca gcatcgcgat ttcttcgtcc atgccaaagc
     2941 gcgcgctttc gctgagttgc aggcgctcgg cataatttat atctttaagg ccatcatttg
     3001 tctcctgggc agcgacccct gccatgctgt ggcaggggtg cccggagttt tgcgtcctgg
     3061 caaaaagagt ggggttcgtg tgttccatct tacgctttct cctttataga atatttgttc
     3121 tatatgtcga taagtgtagc agagaaaaac aaaaaaagaa agtgtcaaaa gtggaaatcc
     3181 gacccccaaa atgcaagaaa gtggaagttt ctttaacatt cagttgctga attttggcat
     3241 atttagggta aacgcaaaaa agccgctgtt ttcactcagc ggctttaggg ttttaaattt
     3301 ttcagtatga cttggtttta cagacttcta cttgtgatgc ttctcgtggt caggagacca
     3361 cgattgaact cccgttcgga tatagtttcc tgaccatgtc catggttcat tgccaggttt
     3421 acgacacgtg caaggcattg acctttctaa agtggtagcc ataaatcga
//
LOCUS       DAOSFC010000148         3428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_25670_length_3428_cov_4.827453, whole genome shotgun sequence.
ACCESSION   DAOSFC010000148 DAOSFC010000000
VERSION     DAOSFC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25670_length_3428_cov_4.827453"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PK791_04550"
     CDS             <1..389
                     /locus_tag="PK791_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92616.1"
                     /translation="KLQELEPVQGGEAFVSLINTKVILDTLALSLKLPTQPFYPGQES
                     ITASSFIEAINATLTYLEEKPTFFTYRMGQEMGEQIPGVLEKLRQLVIESKDLGQDLI
                     LDGYVEAFYLNGEERQTHHIFKIVAD"
     gene            complement(418..2145)
                     /gene="recJ"
                     /locus_tag="PK791_04555"
     CDS             complement(418..2145)
                     /gene="recJ"
                     /locus_tag="PK791_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOH92617.1"
                     /translation="MPKTPRQLAKRWITSPLIPPNISRQMPDVSPFLRQLLYNRGIES
                     NEAALAYISGDSPTNPDPFQMLGMHEAVEVLHDAIQSGQKIAIYGDYDADGVTSSAIL
                     YEFLAQLGLDARVYIPNRFDEGYGLNLEAIEQLADEGVKLLITVDCGISSVAEVARAS
                     ELGMRVIITDHHQVPEVLPDAAAIINPHRPTDPYPYKELAGVGIAWKLVVAYLQRYPN
                     PEIDPDQWLDLVAIGTVVDVAELNGENRSLVKRGLALMRMHPRQGLFSLSQVAGIKLD
                     QLNAGHLGFGIGPRLNAAGRMDSAMAAFELLVTQELFEAARLAQDLDNKNQERKNLLN
                     EIEEIAVQEALASDEDFKVIFVASEDFNQGLVGLAAGRIVESLYKPAIVGHIEGDKIV
                     ASARSIEELNIHETLKECEDLLEKHGGHAMAAGLTLKLENVDAFVERINQVATEKLGD
                     MTLLPILRIDYDIDLARLKPEHIPGILNDVAQLEPTGHKNPDALFSSHHCQALRVRTV
                     GNGAHLKFSVRDGQQEFDAIAFRQGHWVNNMPEYIDIAYTVEINEYMGMRNVQLNVKD
                     IKKSEPKPA"
     gene            2319..3287
                     /locus_tag="PK791_04560"
     CDS             2319..3287
                     /locus_tag="PK791_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOH92618.1"
                     /translation="MEETTKNHQTIHTDVLDSPEVRQARATGAPIVALESTVITHGLP
                     QGENLKLARAMEKTVIENGAIPATVAVIDGRIRLGITNEELEALAVHPNPRKVSIRDF
                     GYTLSKGISGGTTVAATAFVAHKQGVRVFATGGIGGVHRQAPFDVSTDLNQIAKTPLL
                     VVCAGMKAILDLPSTMEYLETQGVPVIGYQTDELPAFYSRSSGIRLNQRADSPLEVAR
                     IAYAHWSLGFRQGIVLTVPVPEKDAIPREEMEGWIDDALIEAQHAGILGNAVTPFLLD
                     KVSQLSGGRSMHSNMALLLNNAIVASQVARELNTLLRHEESQLQLS"
BASE COUNT          869 a          888 c          827 g          844 t
ORIGIN      
        1 ataaacttca agagttggaa cctgtccagg gtggtgaagc atttgtaagt ttaattaata
       61 ccaaagtcat cctcgataca cttgctctta gtctaaaatt accaacccag cccttttacc
      121 cggggcagga aagcatcact gctagcagct tcatcgaagc gattaacgcg actttaacgt
      181 atctcgaaga aaagcccact ttcttcacct accgtatggg acaggaaatg ggcgagcaaa
      241 tccctggtgt tttggaaaaa ctaagacagt tagttatcga aagcaaagac ctggggcagg
      301 atctcatttt ggatggctat gttgaagctt tttacctgaa tggcgaagaa cgccaaacgc
      361 atcatatttt caagattgta gccgactaac agccaaagaa cttaccaaca aaagaaacta
      421 agcgggcttt ggctcgcttt ttttgatatc ttttacattc agctgcacat tgcgcatgcc
      481 catatactca ttgatttcga ccgtataggc gatgtcaatg tattccggca tgttatttac
      541 ccaatgtccc tgccggaaag caatcgcgtc gaattcctgt tggccatcac gcacactaaa
      601 cttcagatgc gccccattac ccaccgtgcg cacccgcaaa gcctggcaat gatggctgct
      661 aaagagcgcg tctgggttct tgtgcccggt tggttccagt tgcgctacat cgttgagaat
      721 gcccggtata tgttccggtt tcagacgcgc aaggtcaata tcataatcaa tgcgcaaaat
      781 gggcaggagc gtcatatctc ccagtttttc tgtcgccacc tggttaatgc gctccacaaa
      841 agcatcaacg ttctccagtt taagcgtcag tccggctgcc atggcatgtc cgccatgctt
      901 ttcgagtaaa tcttcgcatt cctttaaagt ttcgtggatg tttaactctt caatggaacg
      961 ggcggaagcg acgattttat cgccttcaat gtgcccgaca atagcaggtt tataaagtga
     1021 ttcaacgatt ctccccgctg ccaggcccac cagaccttga ttgaaatctt ctgatgccac
     1081 gaagataact ttgaaatcct cgtccgaagc caaagcttcc tgaacggcga tttcttcaat
     1141 ttcattaagc aaatttttgc gttcctggtt tttattgtcc aggtcctgag caaggcgcgc
     1201 cgcctcaaac agttcctgcg tgaccaaaag ttcaaacgcc gccatcgcgg aatccatgcg
     1261 tcccgccgca ttcaagcggg gtccaatgcc aaaaccgaga tgtccggcat tgagttggtc
     1321 aagcttgatg cccgccacct ggctaagcga aaacaaaccc tgacgcggat gcatgcgcat
     1381 gagtgccaga ccacgtttta ccaaagagcg gttttcgccg tttagttcag ccacatcgac
     1441 aacggtgcca atcgccacca ggtcaagcca ctggtcaggg tcaatttccg ggtttggata
     1501 gcgttgcaaa taagccacaa ccagcttcca ggcgatgccc acgccagcca gttctttgta
     1561 aggatacggg tcggtgggac gatgcggatt gataattgcc gcggcatcag gcaaaacctc
     1621 cggcacctga tgatggtcgg tgataatcac gcgcattccc agttcgctgg cacgggcgac
     1681 ctcagccacg gaactgatgc cacagtcaac ggtaattaac agtttcacgc cttcatcagc
     1741 cagttgttca atggcctcga gattaagccc atagccttca tcaaagcggt ttgggatata
     1801 aacccgcgcg tccagaccca actgcgccaa aaattcatat aagatagctg acgatgtcac
     1861 cccatcggca tcataatcac catagatggc gatcttttgc ccggactgaa tagcgtcatg
     1921 caagacttca accgcttcat gcattcccag catctgaaac gggtcaggat tagtaggtga
     1981 gtcgccggaa atataagcca aagcggcctc gttgctttca ataccccggt tgtaaagcaa
     2041 ttgtcttaaa aatggagaga catcaggcat ttggcgactg atgttcggcg ggataagcgg
     2101 tgaagtaatc catcttttag ctaactgtcg gggtgtcttt ggcattgtgt tttccttggt
     2161 ttttctctat tctacctttt tatcttcgct tttcaaaaac atcctgcaaa agctgatgat
     2221 aaaaggtcta aggacataat gaaaccaaat aattttatta cttgtttatg tactggcaat
     2281 attgcgacag aacagggcat gtgttgtaaa attagtgcat ggaagaaaca actaaaaatc
     2341 accaaacaat tcatactgac gtgctggata gccccgaagt acgccaggcc cgcgccactg
     2401 gcgcgcctat cgttgccctt gagtcaaccg tcattactca tggtttgccc cagggagaaa
     2461 acctaaaact tgcccgggcg atggaaaaga ccgtcattga aaacggtgcg atacctgcca
     2521 cagtcgccgt catcgacgga cgcattcgcc tgggtatcac caacgaagaa ctcgaagcgc
     2581 ttgccgtaca ccccaatccg cgtaaagtga gcatccgcga ttttggctac accctgtcaa
     2641 aaggcataag tggcggcacg accgtagcag ccacagcgtt tgttgcccac aagcaaggag
     2701 tgcgggtctt cgcgacaggt ggcatcggtg gcgtgcaccg tcaagcccca tttgacgtct
     2761 ccaccgacct caaccagatt gccaaaaccc cgctgttggt cgtttgtgcc ggcatgaaag
     2821 ccatcctgga tttaccgtcc acaatggagt atctcgaaac ccagggcgtg cccgttatcg
     2881 gttaccaaac cgatgaactc cccgctttct attcccgcag cagtggcatt cgcctgaacc
     2941 aacgtgccga cagtccgctt gaagtcgcgc gaattgctta cgcgcactgg tcactgggct
     3001 tcaggcaagg tattgtcctt accgttcctg tgccagaaaa agacgccatt ccacgggaag
     3061 agatggaagg ctggattgat gacgcgctca ttgaagcgca gcatgcgggc attttgggca
     3121 acgcggtaac gccattcctg ttggacaaag tcagccaact tagtggcggg cgcagtatgc
     3181 acagcaatat ggctctgctt ctcaataacg caattgtggc aagtcaggtt gcacgcgagc
     3241 taaacacact tttgcgccac gaagaaagtc agcttcaact tagttaggtt ttctggctat
     3301 aagtcactgt tgacagtatc ggattaggtg acaatcaggg cgtattcgtt ggccagggcg
     3361 tagacgtgcg cgttggcgtt gatgttggca aggcggtatt cgttggtgtc gcagtttggg
     3421 ttggggtg
//
LOCUS       DAOSFC010000149         3416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_25791_length_3416_cov_5.188337, whole genome shotgun sequence.
ACCESSION   DAOSFC010000149 DAOSFC010000000
VERSION     DAOSFC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25791_length_3416_cov_5.188337"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="PK791_04565"
     CDS             <1..1174
                     /locus_tag="PK791_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOH92619.1"
                     /translation="SFISSAGYMFVLFWATLALPKLRKMHPDAPRPFKVPLFPVTVIL
                     ALLGGFLIIAFADKMALLFLAGIIAFFTIVYFINKHLKSRRKALAALELHEGGGRILI
                     GAKRDGAPESLVRLATQMIGEQTDTTLCLFRVVKTPITLTAAEIEKKIADEKEEQLKT
                     MARVGNLAIEENFPLYTKLVPATRVEEGFTKELQTHKDVRLLLLGFPRDAEKAKLPHN
                     VLKEILMTAQRDVAIVRDKGLPERVKKVLVSLGSGPNVALGLQIVNRMLDHEGETMTA
                     LRVARQELDDEQYEDELAKMQLVADAALGSTPENLEFKLVVANNAIDTILEELKQGDY
                     DLLVLGAGEGLFQPDVLFGFFDDTLLKESPVSVMVVRRYQTESAYWFGQQLKKIEE"
     gene            complement(1329..>1558)
                     /locus_tag="PK791_04570"
     CDS             complement(1329..>1558)
                     /locus_tag="PK791_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010770930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH92620.1"
                     /translation="EDYNPLNELTDREMEVLQEIASGKSNAEIAATLFISEKTVKTHI
                     TNILAKLHLSDRTQAAVLAWQSGVVRRRDGS"
     assembly_gap    1559..1658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1659..2149)
                     /locus_tag="PK791_04575"
     CDS             complement(<1659..2149)
                     /locus_tag="PK791_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH92621.1"
                     /translation="MTEKIRILLVDDHAVVRLGLKSYFATTPDIEVIGEASNGYEAIQ
                     VAQNLLPDVILMDLLMPGMDGVEATRQIKAKLPSTEVIVLTSYHEDENIFPAIRAGAL
                     SYLLKDIDPDDLSNAIRLAARKEAVLNPRVASRLMQEVRGGRRAEDYNPLNELTDREM
                     EVL"
     gene            complement(2228..>3416)
                     /locus_tag="PK791_04580"
     CDS             complement(2228..>3416)
                     /locus_tag="PK791_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOH92622.1"
                     /translation="VHGTEFFVIDKNKMVVASTTQTPGYIVGEAYKPLESNEYEESNI
                     DFLLDIRRSSEPVSFSRLNGAIEILVPLGGYSDEIDYPVWELSYIVGFTVEPAPRFSI
                     VPFLQYLPFVLAIVVLIIVVVTPLGVIFGLIAGRGLTKRISNLAVASEQLAQGNFEAI
                     PLDRSRDEIGQLNRQMRSMSQNLQSLMQSQQQLAAIEERNRLARELHDTVKQQNFATQ
                     MQIRAAQNLIATDPASASARLNEAENLLRSSQDELAVIIRQLRPAQLEDRGLADTLRQ
                     TIRSWCNQTQIPVQVNITGERRIPLEQEQALLRVAQEALSNVARHSRASMVWFGLDYG
                     PNELTMTISDNGVGFGRKPRGGMGLQNMQERIESLGGWFGTSSAPGQGTQVVVRLPLT
                     GLG"
BASE COUNT          758 a          915 c          850 g          793 t
ORIGIN      
        1 gagcttcatc tccagcgcgg gttatatgtt cgtgctcttt tgggcgaccc tggcgcttcc
       61 taaactccgc aaaatgcatc cggacgcccc tcgtcctttc aaagtgcctt tatttcccgt
      121 taccgtcatt ttagccttac tgggtggttt tctcatcatc gccttcgcgg acaaaatggc
      181 tttgctcttc cttgctggca ttatagcctt ctttaccatc gtttatttta ttaataaaca
      241 cctgaaaagt cgccgcaaag cgcttgccgc tctggaattg catgaaggcg gcggacgcat
      301 cctcatcggc gccaaacgcg acggcgcgcc cgaaagcctg gtgcgcctgg caacccaaat
      361 gatcggcgaa caaaccgaca cgaccctgtg tttgttcagg gttgtaaaaa cgcccatcac
      421 cctcaccgct gctgaaatcg agaagaaaat cgccgatgaa aaagaagaac agctcaaaac
      481 catggcgcgc gttggcaatc tggcaattga ggaaaacttc ccgctttaca ccaaattagt
      541 gcctgccaca cgggttgaag aaggctttac caaagaactg caaactcaca aagacgtccg
      601 attgcttctt ttgggcttcc cgcgcgacgc ggaaaaagcc aaacttcccc acaacgttct
      661 caaggaaatt ttgatgaccg cgcaacgcga tgtggctatc gtgcgcgata aaggcttacc
      721 ggaacgcgtc aaaaaagttc tcgtttctct tggaagtggc ccaaacgtgg cgcttggtct
      781 tcaaatagtg aaccgaatgc tcgaccacga aggcgaaacc atgaccgccc tgcgcgtcgc
      841 gcgccaggag ctggacgatg agcaatatga agatgaactc gccaagatgc agcttgtggc
      901 ggatgccgcg ctgggttcaa cacccgaaaa tctggaattc aaactcgttg tggcgaataa
      961 cgccatcgac accattctcg aagaactcaa acaaggcgac tatgaccttt tggtactggg
     1021 cgcgggcgag ggtttgttcc agccggacgt gcttttcggt ttctttgatg acaccttgct
     1081 gaaagaaagc cccgtgagcg tcatggtcgt gcgccgttat caaaccgaaa gcgcttactg
     1141 gttcggccag cagttgaaaa agattgaaga gtaacgcgaa ccagccaagc tgattacagt
     1201 atacagagcg tccgtacaga cgctctgttt ttcacattag attattattg cctgttctga
     1261 cgtggtctcc tgaccacgtt cacgaactta atttacccca ctccgtgtaa gttcttgccc
     1321 ttcaccttct agctaccatc tcgccttctt acaaccccac tctgccatgc caggacggcg
     1381 gcctgggtgc ggtcgctaag atggagtttg gctaatatgt tggtgatatg cgttttgacc
     1441 gtcttttcgc tgataaacag ggtggcggca atttcggcgt tggatttccc gctggcgatt
     1501 tcttgcagca cttccatttc gcggtcggtg agttcgttga gcgggttata gtcctcagnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntg cagcacttcc atttcgcggt
     1681 cggtgagttc gttgagcggg ttatagtcct cagcgcgccg acccccgcgc acttcctgca
     1741 tcaggcgcga agcgacacgc gggttgagca ccgcctcttt gcgcgccgcc aggcgaatgg
     1801 cgttgcttaa gtcatcgggg tcaatatcct tgagcagata cgacaatgcc cccgcgcgga
     1861 tggcgggaaa gatgttttcg tcctcgtgat acgacgtgag cacaattacc tcggtgctgg
     1921 gcaatttggc tttgatttgg cgggtcgcct cgacaccgtc catgccgggc atgagcaggt
     1981 ccatcaggat aacatcgggc aacaggttct gggcgacctg gatggcttcg taaccgttgc
     2041 tggcttcgcc gataacttca atgtcgggtg tggtggcaaa atagcttttt agccccaggc
     2101 gcaccacggc gtggtcatca acgaggagaa ttctgatttt ttctgtcatt gcttttcctt
     2161 tgttaatctt agcataacag caggcttaaa gctcatggtc aggagaccac gggcgaacaa
     2221 aaaacagcta gccaagtcca gttagtggca accgaaccac aacctgcgtg ccctgaccag
     2281 gcgcactgga ggtgccaaac cagccaccca ggctttcgat tctttcctgc atgttttgca
     2341 agcccatacc accgcgcggt ttgcggccaa agccaacgcc attgtcgctg atggtcatgg
     2401 tcagttcatt gggtccataa tccaaaccaa accagaccat gctcgccctg ctgtgacgcg
     2461 cgacgttgct gagcgcttct tgcgcgaccc gcaacaaagc ctgttcctgc tccaaaggaa
     2521 tgcgcctttc accagtgata ttgacctgca ccggaatctg cgtttggtta caccagctgc
     2581 gaatcgtttg acgcagggtg tccgccagtc cgcggtcttc cagctgagca ggacgcaact
     2641 gcctgataat caccgccagt tcatcctgcg aactgcgcaa aaggttctcg gcttcgttca
     2701 ggcgcgcgct ggcgctggcg gggtcggtag ctatcaggtt ttgcgcggcg cggatttgca
     2761 tctgggtggc aaagttctgt tgcttgaccg tatcgtgcag ttcgcgcgcc aggcggttgc
     2821 gctcttcaat cgccgccagt tgttgctgac tttgcatcag cgattgcaaa ttttgcgaca
     2881 tgctgcgcat ttggcgatta agctgcccga tttcatcgcg gcttctatcc agtggaatag
     2941 cctcaaaatt gccctgagcg agttgctccg aagcgaccgc cagattgctg atgcgtttgg
     3001 tgagtcccct gccggcaatc aaaccaaaaa tgacacccag tggcgtgaca acaacgataa
     3061 tcaaaaccac aatggcgagc acaaagggca gatattgcaa gaagggcaca atggaaaagc
     3121 gcggcgcggg ttcaaccgta aaaccgacaa tatagcttag ctcccatact ggatagtcta
     3181 tttcatcact atacccgccc aggggaacga gaatctcgat ggcgccatta agccgcgaaa
     3241 aagacaccgg ttctgacgag cgacgtatat caagcaaaaa atcgatgttt gattcttcgt
     3301 actcgttact ctcaagtggc ttgtaagctt caccgacgat ataacccggc gtttgcgtgg
     3361 tactggccac aaccattttg ttcttatcaa tgacgaaaaa ctccgtgcca tgcacc
//
LOCUS       DAOSFC010000150         3409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_25873_length_3409_cov_4.352713, whole genome shotgun sequence.
ACCESSION   DAOSFC010000150 DAOSFC010000000
VERSION     DAOSFC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25873_length_3409_cov_4.352713"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PK791_04585"
     CDS             <1..330
                     /locus_tag="PK791_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92623.1"
                     /translation="RAFTGYQTVAMFEAATSAPHSDAVLVLDILPTFLDEDLELEDAQ
                     RLFDRALDCLNALSRHAPVVISAKPIPTIARERGMLLDALREQADFCWEEPQTLSAIA
                     MQSSLFS"
     gene            349..783
                     /locus_tag="PK791_04590"
     CDS             349..783
                     /locus_tag="PK791_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92624.1"
                     /translation="MGRTIASITQTWQQEEAALKRFKRALRKEDQILLDELLVLSRLH
                     LAEASYASNLYPLDMYLIAILLEVYKQLKATQRALNDGVDPKEELDASKENRFIALLD
                     ATFSAMQTETQSSPVKTTIIHQQIVETGKPSEICYDDFQESP"
     gene            780..3017
                     /locus_tag="PK791_04595"
     CDS             780..3017
                     /locus_tag="PK791_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HOH92625.1"
                     /translation="MSLKRFTGWLLDLYLDPQNGLKLWFISAEDDSRVCFTQAFPVSF
                     FASGENQRLRALWKRLDKQEGVIKLVRTQKRDVFLNDFVNVLQVDVESPARQTQIFHA
                     LEKDFTDLTWYNADLSVGTRYLARFGAFPLALCELQINENGQIHDIKVCNSRWDIFPA
                     MPPLRVMEITPNCDPIRSKVRYLRVQCDRFQHEIVLDENGVFLKTLHDVLQSYNPDIL
                     VTDWGDSWLIPFLMAESEKRSIRLDLNRDTGRPVRWQKETTYFSYGHIVYRPEEAHLF
                     GRCHVDRKSAMMWSDYSLAGTLEMARVTTLPIEKAARVSPGQGISAMQVITALQRDVL
                     VPYQKRQVEAFKTGMELIQSDRGGMVYQPKVGLHMDVAQIDFISMYPAVIIKSNISPE
                     EPLPDLLEPINAELGLVPATLKPLYDKRVAIKLKTREYPLNHPQRAMLKERANALKWL
                     LVVCFGFLGYKNARFGRIEAHEAVTKGGRELLLRAKEVAEEEGFEVLHMYVDALWIKK
                     KGCNRPEHFRDLIIRINDHTGLAINLDGIYRWLAFLPSKTDSRIPIANRYFGVFQSGE
                     IKLRGIESRRRDTPAWIAGVQKEMIRILAQAKTPSELEQGLCDAFKFYNQALYMLKNG
                     EVDLHELVINGKVSYALDTYKATTPSVRAARQMEVAGMTIKPGQRVRFLYTRGEPDVF
                     AWDLSTSPDPSWLDLAKYIELLAKAGATVLAPFGIEPKLMLEWSQRGGIQLPLASALC
                     YFSDD"
     gene            complement(3158..>3409)
                     /locus_tag="PK791_04600"
     CDS             complement(3158..>3409)
                     /locus_tag="PK791_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92626.1"
                     /translation="YRIYRGTRTCAGEMEVLADVTGTSYIDYEIEYDTTYFYQIQAIE
                     TDGICFSEVLNCATAKVPKPEEPPVYDYRIFLPVSIKAE"
BASE COUNT          917 a          828 c          783 g          881 t
ORIGIN      
        1 cgcgctttca caggctatca gactgtggca atgttcgaag ctgccaccag cgcgccacat
       61 agcgacgctg tgcttgtttt ggacatttta ccgacctttc tcgacgaaga ccttgagctt
      121 gaagacgcgc aacgcctgtt tgatcgcgct ttagactgcc tgaatgcgct tagcaggcac
      181 gcgcccgtgg taatcagcgc caaacccatc ccaaccatcg cccgcgagcg cggaatgctc
      241 cttgacgcct tgcgtgaaca agcagatttc tgctgggaag aaccccaaac tttgtccgct
      301 attgcgatgc aaagcagttt gttttcgtaa aacaggacag aatataagat gggacgcaca
      361 atcgcatcaa ttacgcaaac ctggcaacaa gaagaagccg cgctaaaacg ctttaaacgc
      421 gctttgcgta aagaagacca aattcttttg gacgaactcc tggtgctttc gcgtttgcac
      481 ctggcggaag cttcctacgc gtcaaatctc taccccctgg atatgtacct gattgccatt
      541 cttctggaag tttacaaaca actgaaagca acccaaaggg ccttaaatga tggcgttgac
      601 cccaaagaag aacttgacgc cagcaaagaa aaccgtttta tcgccctttt agatgcaaca
      661 tttagtgcta tgcaaaccga aacgcaaagc agtccggtaa aaacaacaat aattcaccag
      721 caaattgtcg aaacaggcaa gccaagcgag atttgctatg acgacttcca ggaatcgcca
      781 tgagtctaaa gcgtttcacc ggttggcttt tggatctcta tctcgacccg cagaacggtc
      841 tcaaactgtg gttcatctcc gcggaagacg acagtcgggt ctgttttaca caagctttcc
      901 cagtcagttt ctttgcatcg ggagaaaatc aacgactgcg cgcgttgtgg aagcgtcttg
      961 acaagcagga aggtgtcatt aagcttgtcc gtacgcaaaa acgagatgtt ttcctgaatg
     1021 acttcgtgaa cgttttgcaa gtcgatgttg agagcccagc ccgacaaacg caaattttcc
     1081 acgcgcttga aaaggatttt acagatctta cctggtacaa tgccgattta agcgtcggca
     1141 cacgctattt ggcgcgtttt ggcgcatttc ccctggcttt atgtgagctt caaatcaacg
     1201 aaaacggaca aattcatgat atcaaagtct gcaacagccg ttgggacatt ttccctgcca
     1261 tgccaccctt acgcgttatg gaaatcactc ctaactgtga tcccatacgc agcaaagtgc
     1321 gctatttgcg cgtccagtgt gaccgttttc agcatgaaat cgtcttagat gaaaacggcg
     1381 tttttctaaa aacattacac gacgttctgc aaagctacaa ccccgatatt cttgttacgg
     1441 actggggtga cagctggctt attccatttt tgatggctga atcggaaaag cgaagcattc
     1501 gccttgacct taaccgtgac actgggcgtc cagtacgctg gcaaaaagag acaacttact
     1561 tctcttatgg acacatcgtt tatcgtccgg aagaagcaca tctgtttggt cgctgccacg
     1621 tggatcgaaa atccgcgatg atgtggtctg actacagcct ggctggcacg ctggagatgg
     1681 ccagggttac cacattgccc atcgaaaaag ccgcgcgcgt ctcccccggg caaggtatat
     1741 ccgccatgca agtcataacc gcgttgcaac gtgatgtctt ggtgccttat caaaaacggc
     1801 aggtagaagc tttcaaaacc ggtatggaac tcattcaatc cgaccgcggt ggcatggttt
     1861 atcagccaaa agttggtctg catatggacg tggcacaaat tgacttcatc tcgatgtacc
     1921 ctgctgttat catcaagagt aatatctcac ccgaagagcc tttgccagac ctgcttgagc
     1981 caattaatgc cgaattagga cttgtaccag ctacattaaa accgctgtac gacaaacgcg
     2041 ttgccatcaa gctaaaaaca cgcgaatatc ccctcaatca tccccagcgt gccatgctaa
     2101 aagaacgtgc taacgcgctt aaatggcttt tagtagtatg ttttggcttt ttaggctata
     2161 aaaatgcgcg ttttggacgc attgaagctc atgaagcggt taccaaaggc ggtcgtgagc
     2221 tgcttttgcg cgcgaaagaa gtcgccgaag aagaaggctt tgaagtcttg catatgtacg
     2281 tagacgccct atggatcaaa aagaaaggct gcaatcggcc tgagcacttc cgcgatctga
     2341 ttatacgcat caacgaccac accggactgg caattaacct tgatggcatt taccgctggc
     2401 tggcattttt gccttcaaag acagacagtc gcatccccat tgccaaccgc tattttggtg
     2461 ttttccaaag tggcgagata aaactacgtg gcattgaatc acgccgacgc gacacacctg
     2521 cctggattgc tggtgttcaa aaggaaatga ttcggatatt agcccaggca aagacacctt
     2581 cggaacttga acaaggtctg tgtgacgcct ttaaatttta caatcaggcg ctttacatgc
     2641 taaaaaacgg agaagttgat ttgcatgagc tggtgataaa tggcaaggtg agttatgcct
     2701 tggataccta caaagcgacc accccatccg tgcgcgcggc acgtcaaatg gaagtggctg
     2761 gcatgacgat taagcccggt caacgtgtgc gttttttata caccagggga gagccggacg
     2821 tttttgcatg ggatttgagc acaagtccag acccttcctg gttggacctg gcaaaatata
     2881 ttgaactctt agctaaagct ggcgctactg tcctggcacc ctttggcatt gaaccaaaat
     2941 tgatgctgga atggagccag cgtggcggca tacaactccc actcgcgtca gcattgtgtt
     3001 attttagtga tgattagtga aatattgagt tttttatatg taagtgtata ttgttttgtt
     3061 attgtggcta cgcgcattaa cgtgatatgt cataccatag gtgtaaaaaa gagactgcca
     3121 taatgacagc ctcttgacat ttttttagtt tatattttta ttcggccttg atgctaacag
     3181 gcaaaaatat gcgatagtca tatactgggg gttcttccgg ttttgggact ttagcggtcg
     3241 cacaattcag cacctcggaa aaacaaatcc catccgtttc aattgcctgt atttggtaaa
     3301 aatacgtcgt atcgtattca atttcataat caatgtaact tgttccagtt acgtcagcta
     3361 atacttccat ttctccagca caagttctag taccacggta aatacgata
//
LOCUS       DAOSFC010000151         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_25926_length_3404_cov_5.786802, whole genome shotgun sequence.
ACCESSION   DAOSFC010000151 DAOSFC010000000
VERSION     DAOSFC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25926_length_3404_cov_5.786802"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..802
                     /locus_tag="PK791_04605"
     CDS             209..802
                     /locus_tag="PK791_04605"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOH92627.1"
                     /translation="MSDFRAQLTMTSGPVPGSSYYLEKAEFFLGRDLANDIPIPDVEI
                     SRRHARIFLQGNLVFIEDLGSTNGTFVNGTRISSPKELHQGDVITLAESTKFSFSQSG
                     QAVVTPASQTAPMPTHAQASPQAQASYQAVAAPVPERELAPEPAYAKPKKKLSGFLVF
                     LIIFLIILVLAVLVLVFMPSSWWCVITFNGLPGCPVR"
     gene            886..1731
                     /gene="murI"
                     /locus_tag="PK791_04610"
     CDS             886..1731
                     /gene="murI"
                     /locus_tag="PK791_04610"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOH92628.1"
                     /translation="MTKQERQNDPIAVYDSGVGGLSVLRALLEQLPYENFLYFGDQAN
                     VPYGDRSLHELRDLAESVTQFFIAQKVKIIVIACNTASAAALKYLRGIYPEQVFVGME
                     PAVKPAAEQTHTHKVGVLATPSTLHGELYASVVERFAAGVEIFQSTCPGLVAQIEKGN
                     LDGEETLQILHDALDPMLTEGVDSIVLACTHFPFVIPQIEEIVGDRATVIDPAPAIAR
                     QTARVLKQYELLNPQTLAGSRALYTSGNGADLQGMAERLIGLKSTAHELDWHNGQLIS
                     RNSVW"
     gene            1852..2325
                     /locus_tag="PK791_04615"
     CDS             1852..2325
                     /locus_tag="PK791_04615"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HOH92629.1"
                     /translation="MKNKVMSSIVMFGLCVAAVVVVLIGAYLVPLSHQTAETNPTIAH
                     MRVPVLIMGWLILACVIALLVAAFLLLLRINKGNIFEQKSVNLLKLMAVISLAPIPVL
                     ATLFFYTSANVDGSITNFWVVVGVMASVIVSIFFFLLASLFQKAVDYKNENELTI"
     gene            2341..2547
                     /locus_tag="PK791_04620"
     CDS             2341..2547
                     /locus_tag="PK791_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH92630.1"
                     /translation="MAIIVNLDVELAKQKMTLTELSEKVGITLSNLSILKTGKARAIR
                     FDTLNAICAVLQCQPGDLLEFRED"
     gene            complement(2534..3007)
                     /locus_tag="PK791_04625"
     CDS             complement(2534..3007)
                     /locus_tag="PK791_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92631.1"
                     /translation="MSKPLFSKPSAREGETKRPVNHWARLAGFIFLLMALAGFLRVFG
                     LLSLNPVLIAFETSKWVQVYLLVAGFLYGAVNLFAFFCIATVWDKRHIVAWVSALMSI
                     AFYWLERTLFWAPDQRGKAWQFMLALHLAWLIILTLFTRLDIKNKKLAKEDSNLP"
     gene            complement(3004..>3404)
                     /locus_tag="PK791_04630"
     CDS             complement(3004..>3404)
                     /locus_tag="PK791_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92632.1"
                     /translation="RFAEWLELGPNQYSFQLTPASLAKATQSGLSTRHLLSLLRKYGK
                     SASPPTLLKALNRWQEHGAEASIETLTILRLKSPEILQALKDSDANTWLGESLSPVAV
                     VVKPGGVEKVQQALARLGYLSDLEIQSPKK"
BASE COUNT          863 a          819 c          817 g          905 t
ORIGIN      
        1 tcataacctt acctttataa gtcatttttc accgcctaat gataccacca gatgattttg
       61 gtgtaattta tccttctaca ttatgcgcga ataatttaag caaattgttg taaattttca
      121 aaaatccttt ttaaagaaaa catatgtgat ttaaggtaca atagtggtaa ttatccatcg
      181 cacgacacgc ttttattaag gagttatgat gagcgacttt agagcacaat taacgatgac
      241 ttctggcccg gttcccggat cgagttatta tctggaaaaa gccgagtttt ttctcgggcg
      301 tgatttggcc aatgatattc ctatccctga tgtggagatt tcccggcgcc acgcgcgcat
      361 tttcttgcaa ggcaatttgg ttttcatcga agaccttggc tcgaccaacg gcacctttgt
      421 gaatggtacc cgcatcagct caccaaaaga acttcaccaa ggcgatgtca tcacgcttgc
      481 cgaaagcacg aaattcagct ttagccaatc cggacaggct gtggtcacac ctgcttcgca
      541 aaccgccccc atgcccacgc atgctcaggc aagtccacaa gcacaagcca gttatcaggc
      601 cgttgccgcg ccagtgccgg aacgggaact ggcacccgaa ccagcttatg ccaagcctaa
      661 aaagaaactc agtggctttt tggttttcct gattattttc ctgattattt tggtattggc
      721 ggttctggtt ttggtcttca tgcccagttc gtggtggtgt gtgattacct ttaatggcct
      781 gcccggctgc ccggtgcgat aagattgttg tgaacaagag cgctcaatat ctgagcgctt
      841 tttctttttg cacagaaact ccagacatgc cattataata gtgccatgac caagcaagaa
      901 cgccaaaacg atcccattgc cgtgtatgac tccggcgttg gtgggctttc ggttctgcgc
      961 gccttgcttg agcagcttcc gtatgaaaat tttctatact ttggcgatca ggccaatgtg
     1021 ccttatggcg acaggagttt gcatgaactg cgcgatttgg cagaaagtgt gacgcagttt
     1081 tttatcgccc aaaaggtgaa gataattgtc atcgcttgta acactgcttc agctgcggct
     1141 ctaaaatatc tgcgggggat ttacccggaa caagtctttg tgggcatgga acccgctgta
     1201 aaacccgccg cagagcaaac tcacacgcac aaagttggcg tgctggctac gccaagcacc
     1261 ttgcatggcg aactttacgc ctcggtggtg gaacgattcg cggcgggtgt tgagattttt
     1321 caaagcactt gtccgggttt ggttgcccaa attgaaaaag gtaatctcga tggagaagaa
     1381 accttgcaaa ttctacatga cgcgctggac cctatgctga ccgaaggggt ggacagcatc
     1441 gtgctggcgt gcacgcattt cccgtttgtt attccgcaga ttgaagagat tgtcggtgat
     1501 cgtgccacgg tgattgaccc cgcccctgcc atcgcccgcc aaacagcgcg tgtgctcaaa
     1561 cagtacgagc ttctaaatcc acaaacccta gcaggctcgc gcgcgcttta cacttcaggc
     1621 aatggcgcag acctgcaagg catggcggaa aggctcatcg ggctgaaaag caccgcccat
     1681 gaacttgact ggcacaatgg ccagcttatt agccgaaact ctgtctggta atctgtttaa
     1741 tatctgtgaa cttattgaaa tacaacaaaa gtttattgac attcgataaa ctttaagata
     1801 aacttggcgc atcagaacaa aaatatacca ggttctgaca aggagtttat gatgaaaaat
     1861 aaagtaatgt cgagtattgt catgttcgga ttgtgcgtgg cggcagttgt cgttgttttg
     1921 ataggcgctt atcttgtgcc actttcgcat caaaccgcgg agacaaatcc caccatcgcc
     1981 cacatgcgcg ttcccgtgct catcatgggt tggcttatcc tggcttgtgt catcgcgctg
     2041 ctcgtggctg cttttctgct tttattgcgc atcaataaag gcaatatatt cgagcaaaaa
     2101 tctgtcaatt tgttaaaatt aatggctgtc atcagtctcg cgccaatacc cgttctggct
     2161 acgctttttt tctacacgag tgccaatgtg gatggctcaa tcactaattt ttgggtggtt
     2221 gtgggcgtca tggcatccgt tattgtttca attttcttct ttttactggc atcgcttttc
     2281 caaaaagcgg ttgactataa gaacgagaac gaactaacca tttagggaaa cggagagatt
     2341 atggccatta ttgtaaattt agatgtagaa cttgccaaac aaaagatgac cctaaccgag
     2401 ttatccgaaa aagtgggtat cacgcttagc aatctttcca tactcaaaac cggtaaagcg
     2461 cgcgccattc ggttcgatac gctcaatgcg atttgcgcgg tgttgcaatg ccagccgggt
     2521 gatttgctgg aatttaggga agattagagt cttccttggc cagctttttg tttttgatgt
     2581 ccaaacgcgt gaacaaggtc aggatgatga gccatgccag atggagagca agcataaact
     2641 gccaggcttt gccgcgttgg tcgggtgccc aaaacagagt ccgctccaac cagtaaaagg
     2701 cgatgctcat cagggcgctg acccacgcca cgatatgtcg tttatcccaa accgtggcga
     2761 tgcaaaagaa agcgaagaga ttaactgcac catacagaaa tcctgctact aacaagtaaa
     2821 cctgcaccca ttttgacgtt tcaaacgcga ttagcacggg attgagcgat aaaagaccga
     2881 atacccgcaa aaatccagcc agtgccatca ataaaaagat aaaacccgcc agacgcgccc
     2941 agtgatttac tgggcgtttg gtttctccct ccctggcaga aggctttgaa aacaatggct
     3001 tgctcatttt ttcgggcttt gaatttcaag gtcgctcaga tagcccaggc gcgccagggc
     3061 ttgctggact ttttcaacgc caccaggctt tacaacgact gccacgggac tcaaggactc
     3121 cccaagccac gtattagcgt cggaatcttt taaggcttgt aggatttcgg gcgatttcag
     3181 gcgaaggata gttaaagttt cgatactggc ttcagcgcca tgttcctgcc agcgattgag
     3241 cgcttttagc agtgtgggtg gtgacgcgga tttgccatat ttgcgcaaaa ggctcagcaa
     3301 atgtcgcgta ctcaaacccg attgagtcgc tttggccagt gaagcgggcg taagctgaaa
     3361 actgtactgg ttcgggccca gctcgagcca ctccgcaaag cgtg
//
LOCUS       DAOSFC010000152         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_26042_length_3393_cov_5.635111, whole genome shotgun sequence.
ACCESSION   DAOSFC010000152 DAOSFC010000000
VERSION     DAOSFC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26042_length_3393_cov_5.635111"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PK791_04635"
     CDS             <1..812
                     /locus_tag="PK791_04635"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOH92633.1"
                     /translation="VQTFGGKVPSTTDALLKLPGVGPYTAAAIASIAFGVPVAAIDGN
                     IRRVYARLFAITGYLGEKETENQLKEIAQATLSKERPGDFNQALMDLGATICKPRNPL
                     CYECPLKTVCLAFQKGLVSELPRRKEKPKVPHYVVTAAVISDGEKVLIAQRAKDDLLG
                     GMWEFPGGKLEESDVSFHAGLKREILEELGIKIDVGEAVGVYKHAYTHYKITLHAFMA
                     TLQHGESLDRPHGKPECEVFEWVLPEALSKYPMGKVDRLISKQLLGKRLTD"
     gene            992..1228
                     /locus_tag="PK791_04640"
     CDS             992..1228
                     /locus_tag="PK791_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92634.1"
                     /translation="MPYLFSFLTKPVQHIDLGSGRWIYTIILGVIFGLGISIRVYWKD
                     IKKFFTGKEDTNAESDLTETDTIVIDSTDVKNKE"
     gene            complement(1433..2056)
                     /gene="upp"
                     /locus_tag="PK791_04645"
     CDS             complement(1433..2056)
                     /gene="upp"
                     /locus_tag="PK791_04645"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOH92635.1"
                     /translation="MKNVFPSPHPLVSTKLSLLRDVRTEPKKFRELVRELSALLAYEA
                     TLDLATQDIELQTPLAMTQGKRLLERIGLIPVLRAGLGMVEGVWELMPSAEVWHIGLY
                     RDERTLKPVSYYNRLPIEPTVSVCLILDPMLATGGSAVATVDILKEWGVSKIKYIGII
                     AAPEGIERLHNAHPDVPIHIAGIDERLNEHGYILPGLGDAGDRMFGT"
     gene            complement(2053..2406)
                     /locus_tag="PK791_04650"
     CDS             complement(2053..2406)
                     /locus_tag="PK791_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92636.1"
                     /translation="MIFYILVLLFYSAGSYLAFTRDGSELSLWILAFGVVLDIAMLFF
                     DWTGAKFAPRLGEGDLASKIMRILSYFLFGVGFFLRILPKIAGFKLLIALAVAVWLVF
                     FIRSLTLHIKRRKSK"
     gene            complement(2425..>3393)
                     /locus_tag="PK791_04655"
     CDS             complement(2425..>3393)
                     /locus_tag="PK791_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92637.1"
                     /translation="YFVPAHWHNVACPICQKATLVPAAQTRKMPSVELIVKPSLDKAT
                     IDAQLARFCKGSAFPYPDFKPENLSQRLVLVNWPIWLTDGDLKGAWDATFGYDYQVES
                     SREQMGNAGWSSQKVIKTRVKDEPRKGFIERHYDNVLSPALHSHQERLSQIGSYQLNQ
                     ASPGKAEDIATGLTQIGTIAPSELEDFVKQELSKRAAQDCRLASDAQHIRNFTFNGDY
                     NNLRWTQALLPMLASYYTDDQGTRHVVTINGQNGAVYGQRMASVKKAGLITGILLAVA
                     IILMLILYFAKVDFFICSSVLLILAFIPLIRAAGWNNKEKNKRPPA"
BASE COUNT          944 a          732 c          838 g          879 t
ORIGIN      
        1 ttgtgcaaac gtttggtggc aaagtgcctt caacgacaga tgctttgttg aaattgcctg
       61 gtgttgggcc ttataccgcg gcagcaattg cctcaatcgc ttttggtgtg cctgttgcag
      121 cgatagatgg aaacattcga cgcgtttacg cgcgtttatt tgccattacc ggctatttgg
      181 gcgagaaaga aactgaaaat cagctcaaag aaatagcgca agcgactctc tcaaaagaac
      241 gcccggggga tttcaatcag gctttgatgg acctgggcgc gactatctgt aagccccgca
      301 atccactttg ctatgaatgc ccgttgaaga cagtttgtct tgctttccaa aaagggctcg
      361 tttctgaatt gccaaggcga aaagaaaaac ccaaagtgcc acattatgta gtgaccgcgg
      421 cggtgattag tgatggcgag aaagtgctca tcgcgcagcg tgccaaagat gacttgctgg
      481 gtggtatgtg ggaattccca ggtgggaaac tggaagaatc cgatgtgagt ttccatgcgg
      541 gcttaaagcg ggagatactg gaagaacttg gcatcaaaat tgatgttggc gaagcagtcg
      601 gcgtgtataa acatgcctat acccactata aaataacctt gcatgccttt atggcgacat
      661 tacagcatgg cgaatctttg gatagacccc acggaaaacc agagtgtgaa gtttttgaat
      721 gggtgttgcc tgaagcatta agcaaatatc ccatgggaaa agttgaccgg ctaatctcga
      781 aacaactgtt gggtaaaagg ctgacggatt gattttggcg aaaagaacga aatcagctta
      841 aatctatgcc gggaagcaaa atcaaagcgc actaacgaag tccatgtgcc aaaatccagg
      901 ctcatgtgct aaaatgatgt tctgataaca aaatacaaaa aactaaactt agagtctttc
      961 aaacttagaa agaactagct aaagaggtca tatgccatat ttattttctt tccttactaa
     1021 acctgtccag cacatagatc tgggctctgg gcgttggatt tacacaataa tattgggcgt
     1081 tattttcggg ctgggtattt ccattcgtgt ttattggaag gatattaaaa aattctttac
     1141 gggcaaggaa gatacaaacg ctgaatcgga tctgacagaa acagacacca tcgtaataga
     1201 ttcaacggat gtcaagaata aagaataagt tccttagcat tagtattcca gtttaatact
     1261 aaggaacttg aagtgaaaaa gatattgcgc cacgcaaact ggcggatttg ggcgggtatc
     1321 ttatcattga gttcgtgccc aagcatacat aattctaaaa gaagaactca ggcaagcatc
     1381 agaacgacta ttctctgata aatcaaaagt aaaaaagccg gtccggcaaa atttacgtcc
     1441 caaacattct gtcccctgca tcacccagac cggggagaat gtaaccgtgt tcatttagac
     1501 gctcatcaat gccggcgatg tgaataggaa catcagggtg ggcgttgtgc aaacgttcaa
     1561 tgccttctgg cgcggcaatg atgccgatat atttgatttt gcttacaccc cattctttca
     1621 aaatatcaac ggttgccact gctgaaccgc ctgtcgcgag catcgggtcg agtatcaaac
     1681 agactgaaac ggttggttca atgggcagtc tgttgtaata ggaaactggc ttaagcgtgc
     1741 gttcatcgcg ataaagaccg atgtgccaga cttccgcgct gggcattagt tcccatacgc
     1801 cttctaccat gccaagtccg gcgcgtaaaa caggtatgag ccctatgcgt tcaaggagcc
     1861 ttttgccctg ggtcatggca agcggggttt gcaattcgat atcttgtgtt gcaagatcca
     1921 gcgtggcttc gtacgccagt aatgcggaga gttcgcgcac aagttcgcgg aactttttgg
     1981 gttcggtacg gacatcacgc aaaagcgaga gtttggttga gaccaggggg tgtggtgagg
     2041 ggaatacatt tttcattttg atttccttct tttaatgtgt aatgtcaatg aacggataaa
     2101 aaagactaac caaacagcga ctgctaaagc aatcaaaagt ttgaagccgg ctattttagg
     2161 taaaatgcgc aggaaaaagc caacaccaaa cagaaaatag cttaatatgc gcatgatttt
     2221 ggatgccaga tcaccttcgc caagtcgcgg agcaaacttc gctccggtcc agtcaaaaaa
     2281 gagcatggcg atatccagca caacaccaaa agccaaaatc cacagggata actcagaacc
     2341 atcgcgtgtg aaggccagat agcttcctgc ggaataaaac agtaatacaa ggatgtaaaa
     2401 aatcataaac gctttccttt atatttatgc tggcggtcgt ttgttctttt ctttgttgtt
     2461 ccagccggcg gctcttatta gcgggataaa ggccaatatt aagagaaccg aggagcaaat
     2521 gaagaaatca actttggcaa aatagagaat taacatcaaa attattgcca ctgccaaaag
     2581 tatgccggtg ataagtcccg cttttttgac cgatgccatg cgttgtccat agacggcgcc
     2641 attttgtccg ttaattgtca cgacatgccg ggtgccttgg tcgtctgtgt aatagctggc
     2701 gagcatcggc aatagcgctt gcgtccagcg caggttgtta tagtcgccat tgaaagtgaa
     2761 atttcgaatg tgttgggcat cagatgccag gcggcaatcc tgcgctgccc gttttgaaag
     2821 ttcttgtttt acgaagtctt ccaactcact gggcgcgatt gtgccgatct gtgtcagtcc
     2881 agtggcgata tcttcagctt taccagggct tgcctggttg agttgatagc tgccaatttg
     2941 cgacaggcgt tcctggtgac tgtgcagggc aggtgaaagc acattgtcgt aatgcctttc
     3001 aataaaaccc ttgcgtggtt catcttttac gcgggtttta atcacttttt gactggacca
     3061 gcccgcgttt cccatctgtt cacgtgacga ttcgacctga tagtcatatc caaatgtagc
     3121 atcccacgcg cctttcaggt cgccatcggt taaccatatt ggccagttga ctaaaaccag
     3181 gcgttgggag agattttcag gcttgaagtc cggataggga aaagcactgc ctttgcaaaa
     3241 ccgtgccagt tgggcatcaa tcgttgcttt gtccaggctg ggtttaacga tcagttcaac
     3301 agaaggcatc ttgcgcgttt gggctgcagg taccagcgtc gctttttgac agatggggca
     3361 agccacgttg tgccaatgcg cgggaacaaa gta
//
LOCUS       DAOSFC010000153         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_26061_length_3391_cov_4.661571, whole genome shotgun sequence.
ACCESSION   DAOSFC010000153 DAOSFC010000000
VERSION     DAOSFC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26061_length_3391_cov_4.661571"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PK791_04660"
     CDS             complement(<1..570)
                     /locus_tag="PK791_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOH92638.1"
                     /translation="MAQANIIDVVEDNFQMEVLHYSNRMPVVIDFWAPWCVPCKVQSQ
                     KLAGLAVEADGAFRLARINCDEQTKLAEKLKVTSLPHLKAFVDGRVVAEYTGGLSESS
                     LRLFLDRILPQTGSLLLQKGKSLLLQGDYKGAEIALGQYVIESHGEPNGLLAYTRALL
                     FLGQVEDALRFCRVFRQVRNIKPRKDCEYW"
     gene            complement(600..1019)
                     /locus_tag="PK791_04665"
     CDS             complement(600..1019)
                     /locus_tag="PK791_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92639.1"
                     /translation="MKKAIFEGLVYDVNSQPVSVTYVGDEPCYVIDDDGFMRHVSAEK
                     VDRQIWEEMTKDIKGNEDFLSKQAAEMMGQVDPFSVAVIRSQFEKTEEQFAQLQEVGF
                     PEDARMFLGMVGFMAIVDYRGELVELKQPSAISDGDE"
     gene            complement(1149..2177)
                     /locus_tag="PK791_04670"
     CDS             complement(1149..2177)
                     /locus_tag="PK791_04670"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOH92640.1"
                     /translation="MRDIKLTRRRFLQAVGLGVLSFALPAPKPTEARLGKFIPDIPLG
                     RVLKYALPLYKEPDLLSPVLGHYDFDDVIRIPKVFYAETPGSKKRGWYKMAENNYVEA
                     AWIQAVFDKPNRVADEIPQDGCLGEITVPKTPVYRADGQRILTRKFYYGSTFWILDKF
                     KDDFGLSYYELLDDYSGNSWYVRANAVRIVAPEELSLLSPDVAPDAKRIELNMRDKTL
                     RAYEYERLVFETLVTTGLKDGSTPVGQFMTNRKRPCRRMIHEPDNPYTYDLPGVPWVC
                     YITMKGVAFHGTYWHNNWGERMSSGCINMKPTDAKWLYRWTLPHVPFEKYYEVADYGT
                     QVDIIWGY"
     gene            complement(2180..3265)
                     /locus_tag="PK791_04675"
     CDS             complement(2180..3265)
                     /locus_tag="PK791_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOH92641.1"
                     /translation="MEKSDNTTSTLLTQAKQALLAGNRAEAEKLALEILKLDAKNLDA
                     TLIMAGVSEPQESVQWLKQALDLDPGNPTALEGMRWASAAIRQSSAQAWQPALESQAE
                     TSVDEQTQAIVVPAKTETTDKAKPAKPKRKRWFARVFVPLLLVAAIVLFGLYSFGVIR
                     IKPVAGAQFVIKSTYDNFVKPSLTPTNTATPTNTPTSTNTPTPTNTATPTKTPTPSPT
                     PTDTPVPTVAVTPWTPPENQTPPKVFEGKWIDINLSQQMLYAYDGDQLVASFLVSTGL
                     PATPTVTGTYQVWIKLRYDDMVGPGYNLPNVEYVMYFYKGYGIHGTYWHNNFGHPMSH
                     GCVNMRNEDAGWLYNWSYVGITVHVHY"
BASE COUNT          865 a          867 c          747 g          912 t
ORIGIN      
        1 ccagtattcg cagtctttgc gcggtttgat attccggact tggcggaaaa ctctgcaaaa
       61 tcgcaaagcg tcttctacct gccctaaaaa caacaaggcg cgggtatagg ctaacaagcc
      121 attcggctcg ccatgcgact cgataacata ttgtccaagc gcgatctcag cgcctttata
      181 atcgccctgc aaaagcaagc tctttccttt ttgtaacaac aaactgcccg tctgaggcaa
      241 aatgcgatcc aaaaacaggc gcaaactgct ctcgctaagc ccacccgtat actccgcgac
      301 cacacgccca tccacaaatg ctttcagatg gggcaagctg gtcaccttca gcttttcagc
      361 cagttttgtt tgctcatcgc aatttattct cgccagacga aaagcaccat ccgcttcaac
      421 cgccagacct gccagttttt gtgactgcac cttacaaggc acgcaccagg gcgcccagaa
      481 gtcaatcact acgggcatgc ggttggagta atgtaaaact tccatctgaa agttatcttc
      541 aacaacgtcg atgatgtttg cttgtgccat ggtatagtcc tggttttatt ctatcagctt
      601 tattcgtctc cgtccgaaat cgcgctgggt tgtttcagct caacaagttc accgcggtag
      661 tcaacaatcg ccataaaacc caccatgccc aaaaacatgc gcgcgtcttc gggaaaccca
      721 acttcctgta actgggcaaa ctgctcttca gttttctcaa attgagaccg tatcaccgcg
      781 acggaaaaag ggtcaacctg ccccatcatc tccgcggctt gtttgctcaa aaagtcttcg
      841 ttgcccttga tatctttggt catctcttcc cagatttgac ggtcaacctt ttcagctgag
      901 acatgacgca taaagccgtc atcgtcgata acataacagg gctcatcgcc aacgtatgtc
      961 acgcttaccg gctgtgaatt gacatcataa actaatcctt caaatattgc ctttttcaag
     1021 ggtcttcctt tcgttctaag tctgctttta ttcaagttgc caggcgcaac tgaaacaaag
     1081 ccagatttgt gcttttctga ttatagtcta gcacttgggt ataaaaaatg agttaacaaa
     1141 tccccgctct agtaacccca gataatatct acctgagttc cgtaatcggc gacttcataa
     1201 tacttttcaa aaggcacatg gggtaaggtc cagcgataca gccacttggc atccgtgggt
     1261 ttcatgttta tacagccact gctcatgcgc tctccccagt tattatgcca gtaggtacca
     1321 tgaaaggcga cgcctttcat agtgatatag caaacccagg gcacgccggg caaatcatag
     1381 gtgtaaggat tatccggctc gtgaatcatt ctgcgacaag gccgtttgcg attggtcatg
     1441 aactgcccaa ccggcgttga accatctttc aagcctgtag tcaccagcgt ttcaaaaacc
     1501 aggcgctcgt attcataggc acgcagggtt ttatcgcgca tattgagttc aatgcgcttc
     1561 gcgtccggcg ccacatccgg acttagcaga cttagctctt ctggggctac aatacgcacc
     1621 gcgttggcac gcacatacca ggaattccca ctataatcat ccaggagttc ataataagaa
     1681 aggccaaaat cgtctttgaa tttatccagg atccaaaaag tgcttccgta atagaacttg
     1741 cgggttaaaa tgcgttgtcc atcagcccta taaaccggtg tcttgggcac cgttatctcg
     1801 cctaaacaac catcttgggg aatctcatct gctacacgat ttggcttgtc gaataccgct
     1861 tgaatccagg ctgcttcaac atagttgttc tcagccattt tataccaccc tcgtttttta
     1921 ctgcccggag tttcggcata aaagactttt ggaatgcgga tgacgtcatc aaaatcataa
     1981 tgaccaagaa ctggtgacag taaatcaggc tccttatata aaggtaaagc gtattttaac
     2041 acccgtccta aaggaatatc agggataaac ttacctaagc gggcttcagt cggtttcggc
     2101 gctggcaaag caaaactcaa gacgcccagt cccacagcct gtaaaaagcg tcttcgggta
     2161 agtttgatat ctctcatttt taataatgaa cgtggactgt aatccctaca taagaccagt
     2221 tgtacaacca gccagcgtct tcgttacgca tattaacgca accatggctc atcgggtgtc
     2281 cgaaattatt atgccagtaa gtgccatgga tgccatagcc cttatagaaa tacatcacat
     2341 actcaacatt aggcaagtta taaccaggcc cgaccatatc gtcatagcgc agtttaatcc
     2401 aaacctgata tgtcccagtg acagtaggcg tggcaggcaa gccggttgaa accagaaaac
     2461 tggcaaccaa ctgatcaccg tcataggcat agagcatttg ctgactaaga ttgatatcaa
     2521 tccatttgcc ttcaaagact tttggtggcg tttgattttc aggtggggtc cagggcgtca
     2581 cagccaccgt aggcacaggc gtatccgttg gggttggact gggcgtgggc gtttttgtag
     2641 gagtagctgt gttggtcggt gtgggcgtgt tggttgaagt gggtgtgttg gtcggtgtcg
     2701 cagtattcgt tggcgttagg ctaggcttta caaagttgtc ataggtgctt tttattacaa
     2761 actgagcacc cgccaccggc ttgatacgaa tcaccccaaa tgagtaaagc ccaaaaagca
     2821 cgattgcggc aactaaaagc aagggtacaa aaacgcgcgc gaaccatcgt tttctttttg
     2881 gcttggcagg tttagctttg tctgttgtct cagtctttgc cggcacgaca atggcttggg
     2941 tttgctcatc aacgcttgtt tctgcctgac tttccaaagc gggttgccag gcctgcgcgc
     3001 ttgactgacg aatagctgcg cttgcccatc gcataccttc cagtgctgtc gggttgccgg
     3061 ggtcaagatc caacgcctgt tttagccact gtacgctttc ctgtggctca gaaacacccg
     3121 ccataatcag cgttgcatcc agatttttcg catcaagctt gagtatctct aaagcaagct
     3181 tttccgcttc agcgcggttg ccagccaaca aggcttgttt ggcttgggtc agcaaagttg
     3241 aggtcgtgtt gtcagatttt tccatgtttt cttattatag ctaatctgca ttcataaagc
     3301 ggtgaatgcc atcagcgatc ccatctgcta acaattgtgg tcgattagtc aaaatatcct
     3361 ggtcaagatt catataaccc agttcaaaaa t
//
LOCUS       DAOSFC010000154         3372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_26276_length_3372_cov_7.047030, whole genome shotgun sequence.
ACCESSION   DAOSFC010000154 DAOSFC010000000
VERSION     DAOSFC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26276_length_3372_cov_7.047030"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PK791_04680"
     CDS             <1..521
                     /locus_tag="PK791_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOH92642.1"
                     /translation="KFVYNYGTSQVQTGILNAIISAFGGEPVAFLSKVPLNTFMLIIV
                     GIWMWTGFCMTLLSAAIKSIPEEVIEASRIDGATDFEVFAKIQVPIIMPTIIVVITTM
                     VINVLKLFDIVYVMTGGNFGTNVLATRMYTEMYTNSQYGRGTAIAVILILFIVPFIVM
                     NIRRFMKEEAMR"
     gene            518..1531
                     /locus_tag="PK791_04685"
     CDS             518..1531
                     /locus_tag="PK791_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOH92643.1"
                     /translation="MISLTRTKSNGKFDLTRLLTGIGIVLILLVWLIPTVGLLVTSFR
                     PVALINTTGWWTALDVNNGPESAQHPDPNASYVEKLGTRFTINNYVDALTGYRGTKSY
                     LEDCKSGTKPLGMSCSIKDLLNPRGMGRAFLNSVAVTIPSTILPIAIALFAAYALSWM
                     KFKGRYAVFAILVGLQVVPIQMTLVPVLRMFAGMGLNGTFPAVWLFHTGFGLPYAIYL
                     LRNTMSALPKDLFESAYLDGADHWTIFKTIILPLTTPAIASLAIYQFLWVWNDLLVSL
                     IFLGGSKPVMTYQISNMVTSLGAGWHLLTAAAFLSMLLPMIVFFALQRYFVRGMMAGA
                     VKG"
     gene            1649..3277
                     /gene="treS"
                     /locus_tag="PK791_04690"
     CDS             1649..3277
                     /gene="treS"
                     /locus_tag="PK791_04690"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HOH92644.1"
                     /translation="MNKVDKHWYKTAIFMELNVRAYRDSNADGWGDLPGLTSKLDYIK
                     DLGVDAVWLLPIMPSPLRDDGYDVSDFKSIFPAYGTIEDFNTLISEAHKRGIRIIVEI
                     IPNHSSDLHPWFQASRDPNHPEHEKYKDWYVWSDTDQKYKDARIIFTDSETSNWAWDE
                     VRGQYFWHRFYSTQPDLNYDNEEVQQEMLDIVKFWIDRGADGLRVDAPPYLFEREGTN
                     CENLPETHAFLKRMRAFVDEYAPETLLLSEANMWPEEVIEYFGDGDEMHMNFHFPLMP
                     RLFMALARGDRQPIVDILARTPKLPKYCQWGTFLRCHDELTLEMVTPEERQWMWETYS
                     PDPKQRINLGIRRRLAPLLDNDLRKNLMMNSMLLTMIGSPFLYYGDEIGMGDDISLFD
                     RNGVRTPMQWDATPSAGFSDAPKDKWYSPVISDEVYGYEKVNVEAQEADPNSLLKQLR
                     HMIKVRKQYPLFGWAEFEWLDFTEGKQTVVAYKREWEGDYFIVLNNLIDQDVHGTLPA
                     DAPEYLDVLTGETIKAGDYTLPPYGFVWLKPVGE"
BASE COUNT          789 a          825 c          856 g          902 t
ORIGIN      
        1 ggaagttcgt ttataactac ggcacctcac aagtgcaaac cggtattcta aacgcgatta
       61 tcagcgcttt tggcggtgaa ccggttgcat ttttgtctaa agtaccgcta aacacgttta
      121 tgctcattat cgtgggtatt tggatgtgga ccggcttttg tatgaccttg ctttcggcgg
      181 caatcaaatc cattcccgaa gaagtcatcg aagcctcacg cattgatggc gcgaccgatt
      241 tcgaagtttt tgccaagatc caggtgccca tcatcatgcc gacgattatc gttgtgatta
      301 ccacgatggt tatcaatgtg ctcaagctct ttgatatcgt ttatgtgatg accggtggca
      361 attttggcac aaacgttttg gcaacgcgta tgtatactga gatgtacacc aactcgcaat
      421 acggacgcgg tacggctatc gccgtaattc tgatcctgtt catcgtgccg tttattgtta
      481 tgaacatcag acgctttatg aaagaggagg caatgcgatg atttctctga cccgtaccaa
      541 atctaacgga aaatttgatc tcaccaggct tcttactggt atcggcattg tgctcatctt
      601 gcttgtatgg ctcattccaa cggtcggtct tttggtgacg tcgtttagac ccgtcgcgct
      661 gattaacacg accggttggt ggactgcgct ggacgtgaac aacggacctg agtcagccca
      721 acatcctgac cccaacgcca gctatgttga aaaacttggc acgcgtttta ccatcaacaa
      781 ctatgtggac gcgctgacgg gttatcgggg taccaaaagc tatctggaag attgcaaaag
      841 tggcacaaaa ccgctgggta tgagttgttc cattaaggac ttgctcaacc cgcgtggcat
      901 gggacgcgcc ttcctgaata gcgtcgcggt aacgattccg tcgacgattt tgcccatcgc
      961 tatcgcgctt tttgccgctt acgcgctctc gtggatgaag ttcaaaggac gctatgcggt
     1021 tttcgccatt ttggtaggct tgcaagtggt gcctattcag atgacgcttg tgccagtgct
     1081 aaggatgttc gctggcatgg ggcttaatgg caccttcccc gcggtgtggt tattccatac
     1141 tggctttggt ttgccttacg cgatttacct gctgcgcaac acgatgagcg cgctccccaa
     1201 agatttgttc gaatcagcct atttggatgg cgcggaccat tggacgatct tcaagaccat
     1261 cattttgccc cttacgaccc cggcaatcgc gtctttggcg atttaccagt tcctgtgggt
     1321 ctggaatgac ttgctcgtct cgctcatttt cctgggcggc agcaaacccg tgatgaccta
     1381 ccaaatcagc aatatggtga cctcattggg tgctggctgg catttgctca ccgcggctgc
     1441 tttcctttcg atgttgctgc cgatgattgt tttcttcgct ttgcaacgtt actttgtgcg
     1501 tggcatgatg gctggcgcag taaaaggata aagaacttgt tagcaccagt ctgtttatgc
     1561 agattggtgc tatttttttg gggaacagaa aataatatga ttaaaaaata cgccaggtat
     1621 tgcctggcga taacttgaaa ggtgataaat gaataaagtt gacaaacact ggtacaaaac
     1681 tgccatcttt atggaattga acgtgcgggc ttatcgcgac agtaacgccg atggctgggg
     1741 cgacttgcct gggctgacgt ccaagctaga ttacattaaa gaccttggtg tggatgcggt
     1801 ttggcttttg cccatcatgc cttcgccttt gcgtgatgat ggctatgacg tttcggattt
     1861 caagagtatt ttccccgctt atggcacaat cgaagacttc aacacgctta tcagcgaagc
     1921 ccataaacgg ggtattcgca tcatcgttga aatcatcccc aaccacagtt cagacttgca
     1981 tccctggttt caggcttcgc gcgaccctaa tcaccctgag catgaaaagt ataaggactg
     2041 gtacgtttgg tctgacaccg accagaaata taaggacgcg cgcattatct tcaccgacag
     2101 cgaaacttcc aactgggcgt gggacgaagt gcgcggtcag tacttttggc accgctttta
     2161 ttcaacccaa cccgatttga attacgacaa cgaagaagtc cagcaagaga tgctggatat
     2221 cgtcaagttt tggattgacc gtggggcgga cggattgcgc gtagacgcgc cgccttatct
     2281 cttcgaacgt gaaggtacca actgcgagaa tctgcccgaa acacacgcct ttttgaagcg
     2341 catgcgcgct tttgtggatg agtacgcgcc cgaaacgttg ttgctttctg aagcgaatat
     2401 gtggccagaa gaagtgattg aatactttgg cgatggcgat gaaatgcata tgaacttcca
     2461 cttcccgctc atgccacgtc tctttatggc cctggcgcgc ggtgaccgtc agccgattgt
     2521 cgatatcctt gcccgaacgc ccaaactgcc caagtactgt cagtggggca cctttttgcg
     2581 ctgtcatgac gaactgacgc tcgagatggt tacgccagaa gaacgccagt ggatgtggga
     2641 gacctattcg ccagacccca aacaacgcat caatttgggt attcgccgtc ggttagcgcc
     2701 tttgctggat aatgacctgc gcaaaaatct gatgatgaac tccatgcttc tgaccatgat
     2761 cggctcaccg tttttgtatt acggcgatga aatcggcatg ggcgatgata tcagcttgtt
     2821 tgaccgcaac ggggtgcgca cacctatgca gtgggacgcc acgccaagcg ctggtttttc
     2881 ggacgcgcca aaagacaaat ggtactcacc ggtgatttcg gacgaggttt atggctatga
     2941 aaaagtcaat gtggaagctc aggaagctga cccaaactct ctgctcaagc agctgcgcca
     3001 tatgattaaa gtgcgcaagc aataccctct gtttggctgg gctgaatttg aatggctgga
     3061 cttcactgaa ggcaaacaaa ccgtggtggc atataaacgc gaatgggaag gtgattactt
     3121 tatcgtgctc aacaacctga ttgaccagga cgtgcatggc actctccccg cggacgcgcc
     3181 ggaatatctt gatgtgctga ctggcgagac catcaaagct ggtgactata ccctgccacc
     3241 ctatggcttt gtgtggttaa aaccggtggg tgagtaagcc agccaggcga tttcacccct
     3301 gattaattca tcttacccca gcctgattat atacctgtta ggctggggtt tactttaagt
     3361 ttccgaaatt ag
//
LOCUS       DAOSFC010000155         3361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_26400_length_3361_cov_5.251059, whole genome shotgun sequence.
ACCESSION   DAOSFC010000155 DAOSFC010000000
VERSION     DAOSFC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26400_length_3361_cov_5.251059"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PK791_04695"
     CDS             complement(<1..182)
                     /locus_tag="PK791_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92645.1"
                     /translation="MYGAIIGDIVGSQFEGRPAPGKGFEFFTPRCRFTDDTLMTLAIA
                     DAIMQSWHFVSQLPSM"
     gene            complement(308..379)
                     /locus_tag="PK791_04700"
     tRNA            complement(308..379)
                     /locus_tag="PK791_04700"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(344..346),aa:Glu,seq:ttc)
     gene            798..1115
                     /gene="rplU"
                     /locus_tag="PK791_04705"
     CDS             798..1115
                     /gene="rplU"
                     /locus_tag="PK791_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOH92646.1"
                     /translation="MKYAIVEVGGKQYRCEEGKAITVDLINSPVGDEIVLDKVLLIVD
                     DENAIIGKPYIKGASIKTQVQDQIKGKKIFVFKYKPKIRYRRRAGHRQKYTRLLVESI
                     VME"
     gene            1120..1395
                     /gene="rpmA"
                     /locus_tag="PK791_04710"
     CDS             1120..1395
                     /gene="rpmA"
                     /locus_tag="PK791_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOH92647.1"
                     /translation="MAKKKGGGSSRNGRDSRGQRLGIKKFGGEKVLSGNILVRQHGTK
                     VHPGWNVGLGRDYTLFATMDGFVKYETMPTGRKKVHVYETPEGPVRG"
     gene            1399..1878
                     /gene="rpmE"
                     /locus_tag="PK791_04715"
     CDS             1399..1878
                     /gene="rpmE"
                     /locus_tag="PK791_04715"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOH92648.1"
                     /translation="MKKNIQPKYYSNAKVSCASCGAEWETGSTVPEIRTEVCSNCHPF
                     YTGQQARILDREGQVERFYKRLQVRQDLVAEQKAQSEAKTSPDRPLSDLGITAKAVSA
                     LQKANLNVVGDVLKVLEGGEKALLEVDGFGRKSLIDLKKELRRFGYEIPAAAEEIEL"
     gene            1997..2575
                     /locus_tag="PK791_04720"
     CDS             1997..2575
                     /locus_tag="PK791_04720"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOH92649.1"
                     /translation="MAQVSGIVEVITGSMFCGKTEELIRRLRRATIAKQKVVVFKPII
                     DDRYAFNKVTSHSGADFEAVPINHSSEIYDLLKDDVNVVGIDEAQFFDDGIVDVAQTL
                     ADKGLRVIVTGLDTDFRGEPFACMPEMIVKADKVDKLEAICMRCGEPATRTQRLVNGS
                     PANYNDPVVIVGAAEMYEARCRHCHEVPGKGE"
     gene            2578..3141
                     /gene="hpt"
                     /locus_tag="PK791_04725"
     CDS             2578..3141
                     /gene="hpt"
                     /locus_tag="PK791_04725"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOH92650.1"
                     /translation="MAVQKWQDFIKKELISEEELQARIKEMGAEITRDFAGKDLLVIS
                     ILRGGVMITADLIRHIDLPLAVEFMAVASYEQGMRQSDGDVRITLDLRTPIKGRHVLI
                     VEDIIDSGHTLNSVMDLLQTRNPASITICALLDKYERREVDVDIRYTGFKIENEFVFG
                     YGLDIDEYYRNLPFIGTVDLDKYEAPS"
     gene            3125..>3361
                     /locus_tag="PK791_04730"
     CDS             3125..>3361
                     /locus_tag="PK791_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92651.1"
                     /translation="MKHPVSPEFERVLQALREIKADGQKLYLVGGAVRDLLLGKPVHD
                     FDFVTAQGSRELAKALRKKLAGASFALDDERGFNR"
BASE COUNT          927 a          663 c          875 g          896 t
ORIGIN      
        1 tccatactgg gcagctgcga aacaaagtgc cagctttgca tgatggcatc tgcgatggcc
       61 agggtcatca gggtgtcatc tgtaaagcga caacggggcg tgaaaaactc aaaacctttg
      121 cctggagcag gtctgccttc aaattgtgag ccgacaatgt cgccgataat cgcgccgtac
      181 atgtttttcc tttcaatgat ttaattttac ctgagtaatg tttagatttg gcaagtatga
      241 atttttttac tgtgaaggag agaatgtaaa caaaaaggtg ccggatttcc gacaccttaa
      301 gcttttatgc ccctaccggg actcgaaccc gggttttagc cttgaaaggg ctgcgtccta
      361 accgctagac cataggggcg tgaagcaata atatacttga tatttcaaaa tcggtcaagc
      421 attttgtgag aaattgccca aatttgtttt atgtggggat tgaaactcgt aaaattgaat
      481 agtaaggcat gaaaacgatg cgtttgtgaa tgtttacagt aaaaaaacaa gatgttttat
      541 attgtctgtg gggtattaat ctctggtttt gattaccagt atcttttggg gattatgtat
      601 ttgttcctaa aggggtgtca taatcgctgc tcgataacca aaactaatgc aaaatgcttg
      661 actcaaaagc tgtcatcgtt tacaataatt tttcgttgcc ccggcgcaag ttggctgggg
      721 tttgttacgt agaccaactt tcccgtgtat gggcggttgc acgcgcaaaa catggcgaag
      781 tgaagattgg agagaatatg aaatatgcaa tcgtagaagt cggcggtaaa caatatcgct
      841 gcgaagaagg caaagcgatt accgttgatt tgatcaactc acccgttggc gacgaaatcg
      901 tgctcgataa agtgttgctc atcgttgatg acgaaaacgc aattatcggc aagccttata
      961 tcaaaggtgc gagcatcaaa acacaggtgc aggaccaaat caagggtaag aagatttttg
     1021 tcttcaagta caaaccaaaa attcgatacc gccgaagagc tggtcatcga caaaaataca
     1081 ccagacttct ggttgaatca attgtaatgg agtagtgaaa tggccaagaa aaaaggtggc
     1141 ggttcatccc gcaatggtag agacagtcgt gggcaacgcc tcggcatcaa aaaatttggt
     1201 ggcgagaaag tgctcagtgg caatatttta gtaagacaac acggtaccaa agtccatcct
     1261 ggatggaatg ttggtttggg tcgtgactat accttgtttg caactatgga cggttttgtg
     1321 aaatacgaaa ccatgcccac tggacgcaaa aaggtgcatg tttacgaaac acctgaagga
     1381 ccggtaagag gctaagtcat gaagaaaaat attcaaccaa agtattattc aaacgcgaaa
     1441 gtttcctgtg cgtcttgtgg cgctgagtgg gaaaccggtt caaccgttcc tgaaatccga
     1501 accgaagttt gctcaaattg tcatcccttc tacacgggac aacaagcgcg tattcttgac
     1561 cgcgaaggcc aggtggaacg cttctacaaa cgtttgcagg tccgccagga tttggttgcc
     1621 gaacaaaaag cacaaagcga agccaaaact tcgcccgatc gtcccctttc cgatttggga
     1681 atcaccgcaa aagctgtttc cgcgctgcaa aaagctaacc ttaacgtggt tggcgatgtg
     1741 ctgaaggtgc ttgaaggtgg cgaaaaagca ctcttggaag ttgacggttt tggtcgcaaa
     1801 tccttgatcg atctcaagaa ggaactgcgt cgcttcggtt acgaaattcc agccgcggct
     1861 gaagaaatcg aactctaaaa agtttttagt gtaagttgaa ggcagacttg gaaagttcag
     1921 gtctgccttt tgtttttaac tttgccattt tcgatgaaag atttatacta gagatatcat
     1981 aacccttgga ggtaacatgg cacaggtttc tggcattgtt gaagtaataa caggttctat
     2041 gttttgcgga aagacggaag aactcatcag gcgattacga cgcgcgacga tcgcaaaaca
     2101 aaaagtggtc gtttttaaac ccattattga tgatcgttac gctttcaaca aggttacatc
     2161 gcattctggc gcggattttg aagcagttcc catcaatcac tccagcgaaa tttatgactt
     2221 attgaaggat gatgttaatg tggtggggat tgacgaggcg caattttttg atgatggcat
     2281 tgtggatgtc gcgcaaacac tggctgacaa gggcctgcgg gtgattgtga ccggtctgga
     2341 taccgatttt cgcggtgaac cttttgcgtg catgcccgag atgattgtca aagcggacaa
     2401 ggtggataaa ctggaagcaa tttgcatgcg ctgtggcgaa cctgccacac gcactcaacg
     2461 cctggtgaat gggtctccag cgaactacaa tgaccctgtg gtgattgttg gcgcggcgga
     2521 aatgtatgag gcacgctgtc ggcattgtca tgaagtgcct ggaaaaggag agtaataatg
     2581 gctgtacaaa agtggcagga tttcattaaa aaagagttga tttctgaaga agaactccag
     2641 gcgcgcatca aagaaatggg cgcagaaatt acccgtgatt ttgctggcaa ggacctgttg
     2701 gtcatcagta ttttgcgggg tggggtgatg attaccgctg acctgattcg ccatattgat
     2761 ttgcctttgg cagtggaatt tatggctgtg gcatcgtatg agcagggcat gcgccaaagt
     2821 gatggcgatg tgcgcattac gcttgacctg cgcacgccga ttaagggcag acatgttttg
     2881 attgttgaag atattatcga cagtggccat acgctgaatt cagtgatgga cttgctgcaa
     2941 acccgtaatc ccgcttcaat caccatttgc gcgctcttgg ataaatacga acggcgtgag
     3001 gtggatgtgg atatccgcta cactggtttt aagattgaaa atgaatttgt tttcggttac
     3061 ggtctggata tcgacgagta ttatcgcaat ttacccttca taggcacggt ggatctcgat
     3121 aagtatgaag cacccagtta gtcccgaatt cgagcgcgtt ttgcaagccc tgcgcgaaat
     3181 caaagctgat ggacaaaagc tttatttggt gggtggggct gttcgggatt tgttgttggg
     3241 caagccggtc catgattttg attttgtaac cgcgcaaggt tcgcgggaac tggctaaagc
     3301 attacgcaaa aaactggctg gggcgagttt tgcgctggat gatgaacgcg gttttaatcg
     3361 t
//
LOCUS       DAOSFC010000156         3336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_26699_length_3336_cov_4.169156, whole genome shotgun sequence.
ACCESSION   DAOSFC010000156 DAOSFC010000000
VERSION     DAOSFC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26699_length_3336_cov_4.169156"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..547
                     /locus_tag="PK791_04735"
     CDS             170..547
                     /locus_tag="PK791_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92652.1"
                     /translation="MSHNKERIQILSEVRRGELEIPDAITRLQALSARPETPEQEARQ
                     DNSNRELHILRTGLETGKVIMDVRLPLNLLDAAERVGANFAPYLALIPSDTLQECLSI
                     PGTHQLVDIAQEEKGEQLQIFLE"
     gene            666..>3336
                     /locus_tag="PK791_04740"
     CDS             666..>3336
                     /locus_tag="PK791_04740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOH92653.1"
                     /translation="MIAENTQVSPSPERQHWLDKPVLPWWQGLTIEKLLLVIIIVLTV
                     FSRLYDVGARTMAHDEINHVVPAYSFESYVYDPVTHGPFQFHALALSYFLFGDSDFSS
                     RIPAALFGIGVVLFALFAWRRYLGRVGALIAGTLFMISPYILFYSRYTRNEIFIVFWG
                     MVLLWLLLRYMEDGKTKWLYWLVFITAMHYTDKATSFIFSAEALIFLALLFIWKWWRK
                     AWPNDRYHKFFKIAAASTLCLIVFTLGIFILGKTPVANAPMIIDPVTQESVPQHIINS
                     KVPLLISVVFTAIAAVASIVSLIKGLGWYELRKSRLFDLIVLQLVLILPLLVALPVKL
                     VGWDPQDYSTTGILRTGSLFLILLIVSVILGAMWNKKVFLKSLAIFWGIFIVFYTTFF
                     MHGEGFFKGIAGALGYWMSQQSVERGTQPLYYYALVQVPFYEYLPALGTVLALILGFA
                     RKLFLSKTDAIHESPFEKQGLPEPETLEGLEDTALVLEDDAADQEDNPVLPSALTPED
                     INLTDTSDLQDTYDESQPDTENLETTPEETSKPGFFKRLFTDPPLNTEIASDLPILAL
                     LLFWSFMSLLAFSIAGERMPWLTTHITMPMILTASFALGYIVEKIDWAAIKEKKGWLA
                     LIVSFIFILAVLSMFGSLLGTNPPFQGKDLSNMRGTSTFLLSLLGTLGSAIALLHLLR
                     GWQSKQFSKFILLICFALMAIQTARSAFRAAYINYDNAKELLVYAHATRDMKDSVEQI
                     ETISRRLYGDKSIRIAYDNDVRYPYWWYMRDYPNKVDFNDKVTKSLQESPIIVVGSGN
                     FSKIDAVVRDDYYRYDYKRMWWPNEELYRNWTFKKVFNDLKDPAKRAGIWDLWFNRDY
                     NKYASAFGNPSLTLANWSPADGARMYIRKDVAPQ"
BASE COUNT          816 a          947 c          729 g          844 t
ORIGIN      
        1 cattatcgac gtggaggatg aacagagcaa cgaaagagtg cttatctccc tggaatagac
       61 aaacagattt ctaaagacca agctatatcc atgtaaaacg ccctgggaat aatcaaaagc
      121 cagggcgttt tcttataaac ggatatcaaa aatgtgctaa gattaagaca tgagccacaa
      181 caaagagcgc atccaaatcc ttagcgaagt gcgtcgcggc gaactcgaaa tcccggacgc
      241 catcacacgt ctgcaagccc tttcggcccg cccggaaaca cccgaacaag aagcccggca
      301 ggacaactcc aaccgtgaac tgcacatttt acgcactggt ttagaaacgg gtaaggtgat
      361 tatggacgtt cgcctgcccc ttaacctgct tgatgccgct gaacgcgttg gggcaaactt
      421 cgccccttat ctcgcgctta tcccatccga cactctgcaa gagtgcctct ccatacctgg
      481 cacccatcaa ctggtcgata tcgctcaaga agaaaaaggc gaacaactcc aaatattcct
      541 ggaataaaca tattctcaac ccgtaagcac gtaataaaat gccgtagctt catggtgctt
      601 aacttgccga atttccctta gtcgcttata ttttagtatt gagaaaacgc actcggagaa
      661 caactatgat cgcggaaaac acccaagtat ccccatcgcc cgaaagacaa cactggctgg
      721 ataaacccgt tttgccctgg tggcaaggct taactatcga gaaactcctg ctcgtcatca
      781 tcatcgtgct aacggttttt agccgtctct acgacgttgg cgcgcgcacc atggcgcacg
      841 acgaaatcaa ccacgttgtg cccgcctata gctttgaaag ctacgtttat gaccccgtca
      901 cccatgggcc cttccagttt catgccttgg cactttccta cttcctgttt ggcgacagcg
      961 atttctcaag ccgcattccc gccgcgttgt ttggcatcgg cgtggtgctt ttcgcgcttt
     1021 ttgcctggcg tcgctacctg ggcagagttg gcgcgcttat cgccggcaca ctcttcatga
     1081 tttcgcccta cattcttttc tacagtcgtt atacgcgtaa tgaaattttt atcgtttttt
     1141 ggggcatggt tttgctttgg ctgcttttgc gctacatgga agatggcaaa accaaatggc
     1201 tctactggct ggttttcatc accgccatgc actacaccga caaagccact tcctttattt
     1261 tctctgccga agcgcttatc tttttagcct tgctttttat ctggaaatgg tggcgcaaag
     1321 cctggccaaa cgaccgctat cataaatttt tcaaaattgc tgccgcgagc acactctgcc
     1381 tgattgtctt caccctgggc attttcatcc tgggcaaaac acctgtcgcc aacgcgccga
     1441 tgattatcga ccccgtcaca caagagtccg tgccacaaca tatcatcaac agcaaagtgc
     1501 cccttctgat ttcagtcgtc tttaccgcca tcgccgcggt cgcgagcatt gtttctctga
     1561 ttaaggggct gggctggtac gaactgcgca aatcacgtct gtttgacctg attgtcctgc
     1621 aactcgtcct tattctgcca ctcctggtcg ccctgcctgt caaacttgtg ggttgggatc
     1681 ctcaggatta cagcacaact ggtatattgc gaacagggtc gttattcctc atcctgctga
     1741 ttgtttcagt tatccttggc gcgatgtgga acaaaaaagt gttcctaaaa agcctggcca
     1801 ttttctgggg cattttcatc gttttttaca ccactttctt tatgcacggc gaaggcttct
     1861 ttaagggcat cgccggcgcg ctgggctact ggatgtcgca acaatctgtg gagcgtggca
     1921 cgcagccact ttactactac gccctggtac aggtaccctt ttatgagtat ctgccagctt
     1981 tgggcactgt gcttgccctg atattaggct tcgcccgcaa acttttcctt agcaaaaccg
     2041 acgccattca cgagagtcct ttcgaaaaac aaggccttcc tgaacccgaa acgctggaag
     2101 gtttagaaga tacagcactg gtgcttgaag acgatgcagc agaccaggaa gataaccctg
     2161 tactgccatc agctttgacc cctgaagata tcaatttaac agatacaagc gaccttcaag
     2221 acacatatga tgaaagccag cctgacaccg aaaaccttga aacaaccccc gaagaaacgt
     2281 ccaaacctgg ctttttcaaa cgccttttca ccgacccacc cctgaacact gaaattgcca
     2341 gtgatttacc catcctggca cttttgcttt tctggtcgtt catgagcttg cttgccttta
     2401 gcatcgccgg cgagcgcatg ccctggctca ccacccacat caccatgccc atgattttga
     2461 ccgccagttt tgccctgggc tacattgttg aaaaaatcga ctgggctgct atcaaagaga
     2521 agaaaggctg gctggcgctt atcgtcagct tcattttcat tctggcagtc ttgagtatgt
     2581 tcggttccct gctaggcacc aatccacctt tccagggcaa agacctgagc aatatgcgtg
     2641 gcacgagcac tttcctgctg tcgcttttgg gcaccttggg cagcgcgata gcattactcc
     2701 accttttgcg tggctggcaa agcaaacaat tcagcaagtt tattctcctg atttgtttcg
     2761 ccctgatggc tattcaaacc gcgcgatcag ccttccgcgc cgcctatatc aactacgaca
     2821 acgccaaaga attgcttgtc tacgcgcatg ccacgcgcga catgaaagac agcgttgagc
     2881 aaatcgaaac catctcacgc cgtctctatg gcgataaaag catccgcatc gcctacgata
     2941 acgacgtgcg ctacccctac tggtggtata tgcgcgacta tcccaacaaa gtcgatttca
     3001 acgataaggt caccaagtcc ttgcaggaat cacccatcat tgtggtcggt tcgggcaact
     3061 tttcaaaaat cgacgctgtc gtgcgcgatg actactatcg ctacgattac aaacgcatgt
     3121 ggtggcccaa cgaagaactc taccgtaact ggaccttcaa gaaagtcttt aatgacctaa
     3181 aagaccccgc caaacgcgcc ggcatctggg acttgtggtt caaccgcgat tacaataaat
     3241 acgcgagcgc ttttggcaac ccatcgctta ccctggcgaa ctggtctcct gctgatggcg
     3301 cgcgcatgta catccgcaaa gatgtcgccc cgcaaa
//
LOCUS       DAOSFC010000157         3331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_26747_length_3331_cov_4.477717, whole genome shotgun sequence.
ACCESSION   DAOSFC010000157 DAOSFC010000000
VERSION     DAOSFC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26747_length_3331_cov_4.477717"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /gene="infB"
                     /locus_tag="PK791_04745"
     CDS             <1..1251
                     /gene="infB"
                     /locus_tag="PK791_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOH92654.1"
                     /translation="DDGVQPQTREAANHAKAAQVPIIVALNKIDRPDANLERAKTQLA
                     DIGLQPDEWEGDTFVMPVSARTKEGIEDLLETILLVADNIMIKANPKGKILGTVVEAQ
                     LDRTTGPMATLLVQNGTLKVGDVLVAGEGTGRIKAMFDQRQKRVYKAGPSTPVVVMGL
                     NALPSAGDLFEVVANDREGRNIVAARKAEREAKRTVKRVTLEELFKRFKSGEAKELRL
                     ILKADVQGSLEPIINELDKLSEKEKDLSIRVLHAETGNVTESDVMLAAASNAVVMGFA
                     VGMDQGAERLADVEGVSIRLYNVIYRLTEDVEKALKGLLEPEMVEKVTGTANVLALFK
                     VSKGGTAAGCRISSGNITRNAKVRVLRNGKEIAKSEILSLKREKDDVREVRENMECGI
                     TLKDFSDFEVGDVLEAFVMEKFGG"
     gene            1271..1663
                     /gene="rbfA"
                     /locus_tag="PK791_04750"
     CDS             1271..1663
                     /gene="rbfA"
                     /locus_tag="PK791_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOH92655.1"
                     /translation="MPPPLRMKRINDRIKEILSVVLLTKIEDPRLMDVSVTDVRVDRE
                     LDYANIYVSSLDKDADQDEILEALSRASGYLKYEISQEIDLRIMPKLRFFWDPTPQRA
                     DRIDALLAQIRAENEAKEATNEETIDSE"
     gene            1650..2615
                     /locus_tag="PK791_04755"
     CDS             1650..2615
                     /locus_tag="PK791_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH92656.1"
                     /translation="MTANEIIHQRIRAELTQAQNIVITGHIRPDGDAIGSMLGLAQAL
                     REQGKQVQTVLQDPIDPKYSTLIGADGIKDSIEQAYDYLIVVDSADRARVGNVLDDAT
                     IVDLAIDHHISHEDFAEIDFVVADYEATALLIYDCLSAWGLAISKESAECLMTGILSD
                     TIGFRTSSTTPKSLRAVAEMMALGVSLPDVYFKTLSSRSIEELRYWGLGLGKLHYEDG
                     IVWTSLDQQDREKSGFPRYDDADLINHLSSMQGALIAIIFVEQPGGSVKISWRSVPGV
                     DVSRLASKFGGGGHAAAAGADVPGDLATVMEQVLTKSKSYLANRL"
     gene            2636..>3331
                     /gene="truB"
                     /locus_tag="PK791_04760"
     CDS             2636..>3331
                     /gene="truB"
                     /locus_tag="PK791_04760"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOH92657.1"
                     /translation="MDYQENKQKREKLLSKTSGVLVIDKPVGMTSHDVVQAVRRGTGI
                     RRAGHTGTLDPRASGVLVVLLGPAVRLSEYVSTSEKRYQAVIKFGMTTSTYDTEGTIT
                     SQSDDFNFEFEDLEKALEKFVGEVEQTPPIYSALKIDGRRAYDMARKGEEFEMEPRDI
                     TVHSLELLEWEPPEAVVDIHCSSGTHIRSLANDIGQEMGSGATLINLRRTKNGRFTLR
                     DAVSLRKLEEAFAD"
BASE COUNT          881 a          731 c          895 g          824 t
ORIGIN      
        1 gatgatggcg tgcaacccca aacccgcgag gcagccaacc acgccaaggc agcccaggtg
       61 ccgattatcg tggcattgaa caaaattgac cgtccagacg ctaatctcga acgcgcaaaa
      121 acccagttgg ccgatatcgg gttgcaacca gacgagtggg aaggggacac ctttgttatg
      181 cccgtttccg cgcgtaccaa agaaggtatt gaagacttgc ttgagaccat tctgcttgtc
      241 gctgacaaca tcatgattaa ggcgaatcct aaaggtaaaa ttttgggtac tgtggtagaa
      301 gcacaacttg atcgtacaac cggtcccatg gcgactttgc ttgttcaaaa tggcaccttg
      361 aaagtcggcg atgttttggt tgcgggcgaa ggtactggtc gcattaaggc catgttcgac
      421 cagcgccaga aacgtgttta caaagcaggt ccttcaacac cggtggtggt gatgggattg
      481 aacgcgttgc cgagcgcggg cgacctgttt gaagttgtcg ccaacgacag agaaggccgt
      541 aatatcgtcg cggcgcgcaa agcggaacgc gaagccaaac gtaccgtgaa acgtgttacg
      601 cttgaagaac tcttcaaacg cttcaaatca ggcgaagcca aagaattgcg ccttatcttg
      661 aaagcggacg tgcaaggttc ccttgagccg atcataaacg agctggataa actcagcgag
      721 aaggaaaaag atctgtctat ccgtgttttg catgctgaaa cgggcaacgt taccgaaagt
      781 gacgttatgc tggcagcggc gtccaacgcg gtggtgatgg gttttgccgt tggcatggac
      841 cagggcgctg aacgtctggc ggatgttgaa ggcgtttcta tccgccttta caacgtaatt
      901 taccgcttga ctgaagacgt tgaaaaagcc ctgaaaggtt tacttgagcc tgaaatggtt
      961 gaaaaagtca ccggtaccgc caacgtgctg gcactcttta aagtttcaaa gggtggcacc
     1021 gctgctggtt gtcgtatttc ttcggggaat atcacccgga acgcgaaagt ccgtgtgttg
     1081 cgtaatggca aagaaattgc caaatcggaa attctttcac tcaaacgcga aaaagatgat
     1141 gtgcgcgaag tacgtgaaaa catggaatgc ggtatcacgc tgaaagactt ctctgatttt
     1201 gaagttggcg atgtgttgga agccttcgtt atggaaaaat ttggcggata atcacaaaag
     1261 gagactgtaa atgcccccac ctttgagaat gaaaagaatc aacgaccgga taaaagaaat
     1321 tttatccgtc gttttactta cgaaaattga agacccacga ttgatggatg tctcggtaac
     1381 cgatgtgcgt gttgaccggg aacttgacta tgctaatatt tacgtttctt cactcgacaa
     1441 agacgctgat caagatgaaa ttctggaagc cctaagccgt gcgtctggtt atctgaagta
     1501 tgaaatatcc caggaaattg acttgcgcat tatgccaaaa ttgcgtttct tctgggaccc
     1561 aacccctcag cgtgccgacc gcattgacgc tttgctggcg caaattcggg ctgagaacga
     1621 agcaaaagaa gccacaaacg aggaaaccat tgacagcgaa tgaaataatc catcagcgta
     1681 ttcgtgccga actgacgcaa gcccaaaata tcgtcattac cgggcatatt cgccccgatg
     1741 gcgatgcgat tggttccatg ttgggtttgg cgcaggctct gcgagaacag ggcaagcaag
     1801 tacaaaccgt cttgcaagac ccgattgatc ccaagtattc caccttaatc ggtgcggacg
     1861 ggataaaaga cagcatcgaa caagcttatg attaccttat cgttgttgac agtgctgacc
     1921 gtgcccgcgt tggcaatgtg cttgatgacg ctacaatcgt tgatttggcc attgaccacc
     1981 acatcagtca cgaagatttc gcggaaatag attttgtcgt tgcggactat gaggcgaccg
     2041 cgttgttgat ttacgactgc ttgtctgcat ggggtttggc tataagtaag gaaagcgcgg
     2101 aatgcctgat gaccggtatc ctttctgata cgattggttt tcgcacctcc agtactaccc
     2161 caaagtcttt gcgtgctgtg gcagaaatga tggcattggg cgtgagcctg cctgatgttt
     2221 atttcaaaac tttgtccagt cgcagtattg aggagcttcg ctactgggga cttggattgg
     2281 gtaagcttca ctatgaagat ggcattgttt ggacttcgtt agatcagcaa gaccgcgaaa
     2341 agagtggttt tccgcgatat gatgacgcgg atttaatcaa tcatctgtcg agtatgcagg
     2401 gggctttgat agcgattatt tttgtggaac agccgggtgg gtcagttaaa attagctggc
     2461 gatctgtgcc tggtgtcgat gtttcacgac tggcaagtaa atttggtgga gggggacacg
     2521 ccgccgcggc tggagctgat gttccaggtg atttagcgac ggtgatggag caagtattga
     2581 caaaaagcaa gtcctatttg gcaaatagac tataattaat tagctaagag agtgtatgga
     2641 ttaccaagaa aacaaacaaa aaagagaaaa actattgagt aaaacatctg gtgttttagt
     2701 cattgacaaa ccagttggta tgacttcgca tgacgtcgtg caggctgttc gacgtggtac
     2761 tggcattcgc cgtgcgggac ataccggcac tttggatccc cgcgcgtctg gtgtcctggt
     2821 tgtactcctt ggacccgcgg tgcgtctttc ggaatatgtt tctacatccg aaaaacgcta
     2881 tcaggcggtg ataaagtttg gcatgaccac gagcacctat gatactgaag gtacaatcac
     2941 cagtcaaagc gatgatttta actttgaatt tgaagacctt gaaaaagcct tggaaaaatt
     3001 tgttggcgaa gttgaacaaa caccgccgat ttattccgcg ctaaaaattg acggtcgccg
     3061 ggcctacgat atggcacgca agggcgaaga attcgagatg gagccccgtg atatcacggt
     3121 tcattccctt gaactgttgg aatgggagcc acctgaggca gttgttgata ttcattgttc
     3181 gtcggggact catatccgtt cgctggcgaa tgatatcggt caggaaatgg gttccggggc
     3241 gacgctgatt aaccttcgac gcacgaaaaa cggtcgtttt accctgcgcg acgcggttag
     3301 cttgcgcaag ctggaagaag cctttgctga c
//
LOCUS       DAOSFC010000158         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_26815_length_3325_cov_4.245566, whole genome shotgun sequence.
ACCESSION   DAOSFC010000158 DAOSFC010000000
VERSION     DAOSFC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26815_length_3325_cov_4.245566"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /gene="lpdA"
                     /locus_tag="PK791_04765"
     CDS             complement(<1..1139)
                     /gene="lpdA"
                     /locus_tag="PK791_04765"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOH92658.1"
                     /translation="MKTFDVIVIGAGPGGYVAAIRASQLGFKTAIVEKKWLGGVCLNV
                     GCVPSKALLRNAELAHLLRKEAKSFGIKFDNLELDYSEAHKRSRSVSNRLTRGISFLM
                     KKNDIEVIMGSAKLSAKDTVQVQAEDGETLTLKADNIILATGAHAMVIPGMEPDGEKV
                     LDYAQAIMLDKLPESAVIIGGGAIGVEFATVWSGYGVDVHVVEMLPHILPNEDDEAAS
                     ELAKAFRKRGVKVHAGTKVKSVTKTETGTDVLLESEKGEETLSADVTLVAVGFRPNSQ
                     NLGLEEVGVAVNRRGFVEIDERMATSVPGIWAIGDLTGKLLLAHTASAQAVIAAEAIA
                     GLDTPVLDYRMIPRATFTTPQVASFGYTEDQAKEAGFDLKVGKFP"
     gene            complement(1164..1667)
                     /locus_tag="PK791_04770"
     CDS             complement(1164..1667)
                     /locus_tag="PK791_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOH92659.1"
                     /translation="MEFEFKRRDKVNYYLDMAEVVGKRSTCIRKRYGAVIVKNDEVVS
                     TGYVGAPRGRKNCIDMGICRREQLKVPRGERYELCRSVHAEQNAIISASRDRMLGSSL
                     YLTGIDLQDNNNYVEKAMPCAMCKRVIINAGIKTVYIRDNYDEYRKIDVQDWIDNDDS
                     LAAAYGY"
     gene            2013..>3325
                     /locus_tag="PK791_04775"
     CDS             2013..>3325
                     /locus_tag="PK791_04775"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOH92660.1"
                     /translation="MPKMLYLLDGHAIAYRNYFALTSGGTTRFATTDGEPTAGVFGFA
                     STLLKILEQDEPDYLAIAFDKGRTFRDDLYEEYKATREKMPDDLRSQIERIKEMIDLL
                     KIPRLELDNYEADDIIGSLATWAANEEGLGVKIITGDRDLLQLVTERIVVNLPEGRGA
                     KDKDYFPEDVVERMGVKPEQVVDLKALMGDSSDNIPGVRGVGPKTATKLLESYGTLDG
                     VYEHVDEIKGKVGQNIAEQKEQAYLSQTLARIVVDLPVRLDLEQAACENFEPVAAEEL
                     FRELEFRTLIRRLQKLGERYAPGQAQTPQTITPETNSFNLVQGGLFGDEPLDTPDALI
                     SIEDVNTDFETVIVDTPQKLAQAIKDLSAYDTLAFDTETSTLDPVSADLVGFSLAASP
                     EKGYYFPVGHRAGKQLSLDELKSAVQPLLSNPKIAKVAHHAKFDL"
BASE COUNT          807 a          940 c          780 g          798 t
ORIGIN      
        1 aagggaaatt tgcccacctt aaggtcaaag ccagcttctt tcgcttggtc ttcggtgtag
       61 ccaaacgacg cgacctgtgg cgtggtgaaa gtagcccgtg gaatcatgcg gtagtccaaa
      121 accggtgtat ccaagccagc gatagcttct gcggcgatga ctgcctgtgc cgacgcggtg
      181 tgtgccaaga gtagcttgcc ggtcaggtca ccaatcgccc agatgccagg cacgctggtc
      241 gccatgcgct cgtcaatttc cacaaagccc cgtctgttta ccgccacgcc gacttcttca
      301 agccccaggt tttgcgagtt gggtctaaaa ccgaccgcca ccagggtcac atccgcgctg
      361 agcgtttctt cgcctttttc gctttccaaa agtacgtccg tgccggtttc ggtcttggtc
      421 accgatttaa ccttcgtgcc ggcatgcacc ttgacacctc gcttgcggaa agcctttgcc
      481 agttcactgg cagcctcatc gtcttcgttg ggcaggatat ggggcaacat ttcaaccaca
      541 tgcacatcca cgccatagcc actccacaca gtagcaaatt caactccgat ggcaccgcca
      601 ccgataatga ccgcgctttc gggcagtttg tcgagcataa tcgcctgggc gtaatcgagc
      661 accttttcac catcgggttc catgccgggg ataaccatgg catgcgcacc cgtcgccaag
      721 ataatgttat cggcttttag cgtcagcgtt tcgccatctt ccgcctgaac ctgtaccgtg
      781 tctttggcag acagtttggc gctgcccatg atgacttcga tatcgttctt tttcatcaaa
      841 aagctgatac cccgggtaag acggttgctc accgaacggc tgcgcttgtg cgcttcgctg
      901 taatccagtt cgagattatc gaatttaatg ccgaaagatt tggcttcttt gcgcaataga
      961 tgcgccagct cggcgttgcg caaaagcgct ttagaaggca cacaacccac gttcagacat
     1021 acaccaccca gccatttttt ctcaacgatg gcggttttaa agcccagttg cgaagcgcga
     1081 atggcagcca catagccacc tggccccgcg ccgattacga ttacgtcaaa tgttttcatt
     1141 gattcctctc cagttgtatt tttctaatag ccatacgcgg cagcaagaga atcgtcattg
     1201 tcgatccaat cctgcacgtc gatttttctg tattcatcat aattatcgcg aatgtagacg
     1261 gtcttaatcc cagcgttaat aatgacccgc ttgcacatgg cgcaaggcat ggctttttca
     1321 acataattgt tattatcttg cagatcaatg ccagtgagat acaaggaact gcccagcata
     1381 cggtcgcggg acgcgctgat tatcgcgttt tgctcagcat gcacactgcg gcacagttca
     1441 tagcgttcgc cacgtggcac cttgagttgt tcccgccgac agatgcccat gtcgatacag
     1501 tttttgcgtc cgcgtggcgc gccaacgtaa cccgttgaga ccacttcatc gtttttcaca
     1561 atgaccgcgc catagcgctt gcgaatgcag gtgctgcgtt tgccaaccac ttcggccatg
     1621 tcaagataat aatttacttt atcacgtcgt ttgaactcaa attccatctt cactccgctt
     1681 gtttttgtaa tgatagactt attatagacc aaacggcgac taaatttcag taatgtgtcc
     1741 aaccgcgttg tttacccagc aactgaaatg atgccatatg tattaaaaaa gacccgcccc
     1801 ctttaatccc cctcctctcg cgtcgcgaaa tgaccgttta ttctcaaata atttgtatac
     1861 cgcgacgctg gatgggggga atgctttcgc taacattcag aatactcatc cctgacagac
     1921 atttaaagtt cagttgcccg ggcccgcctt gcgatgccct cgcgtagatg ctacacttaa
     1981 gccatctata acgcagtcaa ctggaggaaa ttatgccaaa aatgctttat ttgctggacg
     2041 gacacgccat cgcctaccgc aattacttcg cgcttaccag tggtggcaca acccgcttcg
     2101 ccacaaccga cggcgaaccc accgcgggcg tttttggctt tgccagtaca ttgctgaaaa
     2161 tcctcgaaca agacgaacct gattatctcg ctatcgcctt tgacaaaggc agaactttcc
     2221 gcgatgacct gtatgaagag tacaaagcca cccgcgagaa aatgcccgat gatctgcgtt
     2281 cgcaaattga acgcatcaag gaaatgattg acctgctcaa aatcccccgt cttgagctgg
     2341 ataattacga agcggatgac atcatcggca gtcttgccac ctgggcagcc aacgaagaag
     2401 gcttgggcgt caaaatcatc accggcgacc gcgacctttt gcaactggtt actgagcgca
     2461 ttgtcgtcaa cctgcccgaa ggacggggtg ctaaagataa agattacttc cccgaagatg
     2521 tcgttgaacg catgggggta aaacccgaac aggtggttga cctgaaagcc ttaatgggcg
     2581 acagttccga caacatccct ggtgtgcgcg gtgttgggcc caaaacggcc accaaactgc
     2641 tggaaagcta tggcaccctg gatggtgttt acgagcatgt ggacgagata aagggcaaag
     2701 tgggccaaaa cattgccgaa caaaaagagc aagcctatct cagccaaacg ctggcgcgca
     2761 tcgtggtgga tttgcccgtg cgactggacc ttgaacaagc cgcttgcgaa aattttgaac
     2821 cggttgcggc ggaagagctt ttcagagagc tggaattccg cacgcttatc cgacgtttgc
     2881 aaaaactggg cgaacgctac gcgcccggcc aagcccaaac cccgcagaca atcacgcctg
     2941 agacaaacag cttcaacctg gtgcaaggtg gtttgtttgg cgatgagccc ctggatacac
     3001 cagacgcttt aatcagcata gaagacgtca ataccgactt tgaaaccgtc atcgttgata
     3061 caccccaaaa gcttgctcag gcaataaagg acctttcggc atacgacacc ctggcattcg
     3121 acaccgaaac cagcactttg gatcccgtct ctgctgattt ggttggcttt tccctggctg
     3181 ccagcccgga aaaaggctat tatttcccgg tcggacaccg tgccggtaaa caactcagcc
     3241 ttgatgagct caaatccgct gttcaacctc tacttagcaa ccccaaaatc gccaaagtcg
     3301 cccatcacgc caaatttgac ctaat
//
LOCUS       DAOSFC010000159         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_26986_length_3309_cov_5.974186, whole genome shotgun sequence.
ACCESSION   DAOSFC010000159 DAOSFC010000000
VERSION     DAOSFC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26986_length_3309_cov_5.974186"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1106
                     /locus_tag="PK791_04780"
     CDS             42..1106
                     /locus_tag="PK791_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOH92661.1"
                     /translation="MTKPTLPKIDYAYLETFLVDLLNIPSPSGYTDAAISFVEEALKD
                     FPEARAERTKKGSLLIHVPGETSHEAGPRRALTAHVDTLGAMVKEIKSNGRLELTELG
                     GYAWGSVEGENLLVHTRHHGTVRGSVLPNKASVHVYKTVRDDKRSADSMEIRLDKRVA
                     NPDDVRALGIEVGDFVSFDPRVELHDGFVKSRHLDDKAAVAVVMAVIKAFHDSGTKPL
                     EDFCVLISTYEEEGMGGRSDLPASLEELIAIDMAAIGAGQASDEFHASICVKDAAFPY
                     HHGFSNKLRDLAEAYDIPHKVDIYVNYSSDATTYWSAGGSAAVALIGPGVDSSHHYER
                     THKDALMGTTRWVLAYLLNE"
     gene            1241..1495
                     /locus_tag="PK791_04785"
     CDS             1241..1495
                     /locus_tag="PK791_04785"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOH92662.1"
                     /translation="MFFQNMHWLWAILVGAVIGLLADMVVPGVKLGFLGAIIVGLIGG
                     LVGGWLSSLLSFGGPLWVQLIFAFIGAVIVLLIWRSIKRK"
     gene            1675..2163
                     /locus_tag="PK791_04790"
     CDS             1675..2163
                     /locus_tag="PK791_04790"
                     /inference="COORDINATES: protein motif:HMM:NF025384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HOH92663.1"
                     /translation="MSYQQPQQPQQPYQQQPYQQQPYQQQPYPPQMTKLRTDFSMGKT
                     VLLSLITFGIYGIVVWCRLVDNINTLATPYDRKNTMNYFLAILLGIITLGIYPIVWNH
                     QLCDRIGDELHRRRIGYELSSSTYWLWGVLGSLIIVGPFIYMDKVFNATNMLSQHYNQ
                     FG"
     gene            complement(2233..3219)
                     /locus_tag="PK791_04795"
     CDS             complement(2233..3219)
                     /locus_tag="PK791_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH92664.1"
                     /translation="MFDTQKTGLKIMTLRKAKDMTQMELADLMQVSYQAVSNWERGNS
                     MPDISKLADLARILDVSIEELLDSRQQAQTVKRALDENETLTAKEITEVAPLLKPSKL
                     EKELEKAPVEGLELKDLQQLAPFLSEEKLMELVERVGFNRVSDLVPVAPFMNSKQLQT
                     IIIQQGELHENLDDIISIVPFLEESFVADIAKNLIADDDDASLLLPLVPFLDTDTLMD
                     LIEEAEPENMNDILPFMPFLKSEEIMRLLAKLSKSLRMDDLSKIAPFLDEDALSQLVD
                     KAIDSGEKAQDIENIVTHLNPKTVKKYAGKLIEKGDIKTLKSILPFLEDNVD"
BASE COUNT          850 a          764 c          735 g          960 t
ORIGIN      
        1 cgccaaaaat tatcagaata cgaaaacaac aaggatttac tatgaccaaa cccacattac
       61 ctaaaattga ttacgcttat ttggaaactt ttttagttga tttgctcaac attcccagcc
      121 catccggcta caccgatgcc gcaatcagct ttgttgaaga agccctgaaa gatttccctg
      181 aagcgcgtgc tgaacgcacc aaaaaaggca gtttgcttat tcacgttcct ggcgagacat
      241 ctcatgaagc tggcccgcga cgggcattaa ccgcccatgt ggatactctt ggcgcgatgg
      301 ttaaggaaat caaaagcaac ggccgcctgg aactcaccga actgggcgga tacgcctggg
      361 gcagcgtgga aggcgagaac ctgttggtgc atacccgtca tcatggcact gtgcggggca
      421 gcgtcttgcc caacaaagcg tcggtgcatg tttataaaac cgtgcgcgat gacaaacgca
      481 gcgcggacag catggaaatc cgcctggata aacgtgtggc aaaccccgat gatgtacgcg
      541 ccctgggcat cgaagttggc gactttgtct cgtttgaccc gcgcgttgaa ctgcacgatg
      601 gttttgtgaa gagtcgccac cttgacgaca aagccgctgt cgcggttgtg atggctgtca
      661 ttaaagcttt ccacgactcg ggtacaaaac cgcttgagga cttctgcgtg ctgattagca
      721 cctacgaaga agaaggtatg ggtggtcgca gcgacttgcc agccagtctg gaagaactca
      781 tcgctattga catggctgct attggcgctg ggcaggcatc ggacgaattt cacgcgtcca
      841 tttgcgtaaa agatgctgcc ttcccttacc atcatggctt ttcaaacaaa ctgcgcgacc
      901 ttgctgaggc atacgatatc ccccacaagg ttgatattta cgtgaattac agctccgacg
      961 ctacaaccta ttggtcggcg ggtggttcag cggcagtcgc tttgattggc cctggtgtgg
     1021 acagttcgca tcactacgaa cgtacccaca aagacgcgct catgggcact acaagatggg
     1081 tcctggcgta cctgctgaat gagtaataat tggcgcataa gccagcgttg taaagctcat
     1141 tttgaaacct ttgctaagct catgggtttt taatgatgac cgcgcctaat cattaaggcg
     1201 tggttcaaaa aaactatttt gaaaacaaag gagagttaat atgttctttc aaaatatgca
     1261 ttggttatgg gcgattttag tcggtgctgt catcgggctc ctggcagaca tggttgttcc
     1321 tggtgtaaaa ctcggctttc ttggcgcgat tattgttggt ctcatcggcg gtctcgttgg
     1381 tggctggctt tcttcgttgc ttagctttgg tggcccactt tgggttcagt tgattttcgc
     1441 ctttatcggc gcggtgatcg tcctgcttat ttggcgctcg attaaacgta agtaaaaaac
     1501 aattatctaa tttccacaaa aacgcgccag ttaactggtg cgtttttgta tgctcaagcc
     1561 aacaatacaa tacaatttat ttatgaaaag tttgctttgt aaatactatt aacaatgtat
     1621 atccgagcct gtttactgtg gtatcattaa gaaaattaat cttatggagg gcacatgtct
     1681 taccaacaac cacaacaacc acaacaacct tatcaacagc aaccttatca acaacagccg
     1741 taccagcaac aaccttatcc tccccaaatg actaagttaa gaactgactt tagcatggga
     1801 aaaaccgttc tcttgtccct tatcactttc ggtatttatg gcatcgtcgt atggtgtcgt
     1861 cttgtggata acatcaatac acttgcaacg ccttatgacc gaaaaaacac catgaattat
     1921 ttcctggcaa ttctcctggg catcatcacc cttggcattt atccaatcgt ttggaaccat
     1981 cagctttgtg accgcatcgg agatgaactc catcgacgcc gtatcggtta cgaattaagc
     2041 agctcaacat attggttatg gggtgtttta ggctcattga tcattgttgg accattcatc
     2101 tatatggata aggtcttcaa cgcgaccaac atgctctcgc aacattacaa tcaatttgga
     2161 taaaccaaaa ccattcgttt aaagagaaaa gcgtactgtc aaaagtacgc tttttttatc
     2221 gtaatagttt gtctagtcta cgttgtcttc caggaatggc aaaatggact tcagagtctt
     2281 aatatcacct ttttcaatta atttcccggc gtattttttg accgtttttg gattaagatg
     2341 agtgacaatg ttttcgatgt cctgcgcttt ctccccgctg tcgatagcct tatcgacaag
     2401 ctggctgagt gcgtcttcgt ctaaaaatgg ggcgatttta gacaaatcat ccatgcgcaa
     2461 ggatttgctt agcttagcaa gcaaacgcat aatctcttcg cttttcaaaa atggcataaa
     2521 tggcaaaatg tcattcatat tttcaggttc agcttcttca atcaagtcca ttaaggtgtc
     2581 tgtgtcaaga aatggcacca aaggaagcaa gagactggcg tcatcatcat cggctatcag
     2641 gtttttagcg atatccgcaa caaagctttc ttccagaaaa gggacgatgc tgataatatc
     2701 atccagattt tcgtgcaatt cgccttgctg aatgatgata gtttgcaatt gcttgctgtt
     2761 cataaatggg gcgacaggaa ctaagtcgct aacgcgatta aagccaacac gctccaccag
     2821 ttccattagc ttttcttcgc tcaaaaaggg tgccagttgc tgaagatctt tcaattcaag
     2881 gccttcaaca ggtgcttttt caagttcttt ttccagcttt gaaggcttta atagtggcgc
     2941 gacttcggta atttctttgg cagtcagggt ttcgttctcg tccagggcgc gtttaacagt
     3001 ttgggcttgc tggcgggaat caagcagttc ttcaatactc acatccagta tgcgtgccag
     3061 gtcagcaagc ttcgaaatat ctggcatgct gttgcctctt tcccagttgc tcactgcctg
     3121 atagcttacc tgcattaaat cagccagttc catttgagtc atatctttgg ctttgcgcag
     3181 ggtcataatt tttaagcctg ttttttgtgt gtcgaacatc attcactcct tgttttggtt
     3241 taggtttatc ttagaccaaa acgtttctta atcctatcaa gcattgcttg aatttgggcg
     3301 attcaatta
//
LOCUS       DAOSFC010000160         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_27002_length_3308_cov_4.718414, whole genome shotgun sequence.
ACCESSION   DAOSFC010000160 DAOSFC010000000
VERSION     DAOSFC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27002_length_3308_cov_4.718414"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1605)
                     /gene="purH"
                     /locus_tag="PK791_04800"
     CDS             complement(85..1605)
                     /gene="purH"
                     /locus_tag="PK791_04800"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOH92665.1"
                     /translation="MKYALISVFDKSGIVEFARSLRDLGFGLISTGGTFKLLKEQGIE
                     VSEVSEITQFPEMMGGRVKTLHPRVHAGILYRRDVPEDIEALQAHQINAIDIVVNNLY
                     PFEEMLHQGKDDATMIENIDIGGPSMIRAAAKNHKDVTIVVDPQDYAEVIQRLQDGTL
                     DEAYRRQLAAKAFSLTAYYDGIISSYFNSLLGNKFPERFARPLKLEAGMRYGENPHQA
                     AALYRDGFQPARTTFDFEQLHGKEISYNNMNDLTGAVMMLKEFDRPCAVGVKHSNPTG
                     IACDDDINIAFDKMIACDDVSIFGGIIAINRAVTAHIAERINTMFMEIVIAPDFEPEA
                     FDILAKKKNIRLIKSPNIMQAQFPDLKYKDVINGTLLQENDKVLYQAFNVVSKRQPSQ
                     KEIDDMVFGMKAVKHISSNGVVLVKDEGTVGYGFGEVRRSWAVEKAIERGAGHLEGSV
                     LASDAFFFEDTVELLHANGIKAAISPGGSIHDQAVIDLCDQYGIALVFTETRHFRH"
     gene            complement(1602..2234)
                     /gene="purN"
                     /locus_tag="PK791_04805"
     CDS             complement(1602..2234)
                     /gene="purN"
                     /locus_tag="PK791_04805"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOH92666.1"
                     /translation="MRALKIAVLASGGGTNLQALIDAQAAGKINGTIELVISNRKNAY
                     ALERARLANIACEHISAKSFPDPIDYDKALLSRLKAHQIDLIVLAGYLKILSPVLIQA
                     YQHKIINIHPSLLPAFGGDGFYGIHVHNAVYQNGAKVSGATVHFVTEGVDAGPIILQK
                     AIPLEQNWQATDIQAAVLKIEHPLLVEAVSLFCDNRLEIKENRVYIQGEL"
     gene            complement(2222..3256)
                     /gene="purM"
                     /locus_tag="PK791_04810"
     CDS             complement(2222..3256)
                     /gene="purM"
                     /locus_tag="PK791_04810"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOH92667.1"
                     /translation="MTLSYKDAGVDKEAGYREVKLIKEMVKKTFSPAVISDLGGFGGL
                     FKLDTAAYQEPVLVSGTDGVGTKLDIAFRMDKHDTIGIDAVAMCANDILCQGAKPLFF
                     LDYIATGKLVPEKMAQIVEGVVEGCMQAEMALIGGETAEMPGIYQENHYDIAGFAVGI
                     VDKAKMIVPGKVQIGDHVIGLKSSGVHSNGFSLVRKIIFDIHNFSLDTHMDALGMSLG
                     EALLTPTKIYVKDLMPIIEHFDLHAIAHITGGGIYENLPRVLSNGQGLRWDVSSYEIP
                     PIFRLLEEWGNVDRKEMFGTFNMGIGMILVLSPRDIDGVLASLNARNIPYIDMGTITE
                     GEKDVQLCGL"
BASE COUNT          769 a          803 c          877 g          859 t
ORIGIN      
        1 accccagaat tttgagcaag tttccaggcg atggcgtgtt cgcgcgcgcc actgccaaca
       61 ataagaatct tcatcgagcc tcttttagtg tctaaagtgg cgggtttcgg tgaacacgag
      121 cgcgatgccg tactgatcgc acaagtcgat cacagcctgg tcgtgtatag aaccgccggg
      181 tgaaatggcc gctttgatac cattggcgtg tagcagttca acggtgtctt caaagaagaa
      241 tgcgtctgat gccagtacgg agccttccag gtgaccggca ccgcgttcaa tggctttttc
      301 aaccgcccac gagcgacgca cttcgccaaa accgtagcca accgtgcctt cgtctttgac
      361 caaaacaaca ccgttggagc tgatgtgctt gacggctttc ataccaaaga ccatgtcatc
      421 aatctctttt tgactgggct ggcgcttgga aacgacattg aaggcttgat aaagcacttt
      481 gtcattttcc tggagcaagg tgccattgat gacgtctttg tatttcaggt cagggaattg
      541 cgcctgcatg atattcgggg atttaatcag gcggatgttt ttcttcttcg ccagaatatc
      601 aaaagcctcg ggttcaaaat ctggagcgat aacgatttcc atgaacatgg tgttgattct
      661 ttcagcgatg tgggctgtta ccgcgcggtt gatggcgatg atgccaccaa agatagacac
      721 atcatcacag gcaatcattt tgtcaaaagc gatgttgata tcgtcatcgc aggcgatgcc
      781 ggtggggttg ctgtgcttca caccaaccgc acaggggcgg tcaaattcct tgagcatcat
      841 caccgcgccg gtgaggtcgt tcatgttatt gtatgaaatt tctttgccgt ggagttgttc
      901 aaagtcaaag gttgttcgcg cgggctgaaa accgtcacgg taaagagccg cggcttgatg
      961 ggggttttcg ccatagcgca tgcccgcttc aagtttgagc ggtcgggcaa agcgttctgg
     1021 gaacttgttg ccaagcaaac tgttgaagta gctggagata atcccgtcat aatacgcggt
     1081 aagactgaaa gctttggctg ccagttgtct gcggtaggct tcatcgagcg tgccatcttg
     1141 caagcgttga atgacttcgg cataatcctg cgggtcaacc acgatagtga cgtctttgtg
     1201 gtttttggca gcagcgcgaa tcatgctggg tccgccgatg tcgatgtttt caatcatggt
     1261 ggcgtcatct ttgccctgat ggagcatttc ttcgaaagga taaaggttgt tgaccacgat
     1321 atcaatagcg ttgatttgat gcgcctgcaa ggcttcgata tcttcaggaa cgtcgcggcg
     1381 gtaaaggatg ccagcatgca cgcggggatg aagcgttttg acgcgtccgc ccatcatctc
     1441 cgggaattgg gtgatttctg aaacttcgct aacttcaatg ccttgttctt tcaaaagttt
     1501 gaaggtgcca ccggtagaga tgagaccaaa gcccaaatct cttaaactcc gcgcgaattc
     1561 aacgatgccg gatttgtcaa agacggatat taatgcgtat ttcataactc tccttgtatg
     1621 taaactctgt tttcttttat ttcaagacgg ttatcgcaaa acaagctgac cgcttcaacg
     1681 agcaaagggt gttcaatctt gagtaccgct gcctgtatgt cagtcgcctg ccagttttgt
     1741 tcaagcggaa tggctttttg cagaatgatt ggaccggcgt caacgccttc ggttacaaag
     1801 tgcacggtcg cgccggaaac tttcgcgcca ttttggtaaa cggcattgtg cacatgaatg
     1861 ccataaaacc catccccacc aaaagcgggg agcagtgagg gatggatgtt gatgattttg
     1921 tgttggtatg cttggataag caccgggcta aggattttga gataccctgc caggacaatc
     1981 aggtcaatct gatgcgcttt tagccgggaa agcaaggcct tatcgtagtc gatggggtct
     2041 ggaaagcttt tcgcggagat gtgttcacag gcgatgtttg ccaggcgcgc gcgttcgagc
     2101 gcgtaagcgt ttttgcggtt tgagatgacc agttcaatcg tcccgttgat tttccccgcg
     2161 gcttgcgcgt caatcagcgc ttgcaaattc gtgccaccgc ctgatgccag caccgcgatt
     2221 tttagagccc gcaaagttgc acgtcctttt cgccttcggt gatggtgccc atatcgatgt
     2281 atgggatgtt ccgggcgttc agactggcga gcacgccatc aatgtcccgc ggtgaaagca
     2341 ccaaaatcat gccgatgccc atattgaaag taccgaacat ttctttgcgg tccacattgc
     2401 cccattcttc caaaaggcgg aagatgggcg ggatttcata actggaaaca tcccagcgca
     2461 agccctgacc gttggaaagt acgcggggca agttctcgta aatacccccg ccggtgatat
     2521 gagcgatggc gtgaaggtca aagtgttcga tgatgggcat aaggtctttg acgtaaattt
     2581 tggttggggt caaaagcgct tcgcccaggg acatacccag ggcgtccatg tgggtatcaa
     2641 gcgaaaagtt atggatgtca aaaataattt ttcgtaccag tgagaacccg ttggagtgca
     2701 caccgcttga tttcagtccg atgacgtggt cgccgatttg caccttgccg ggcacaatca
     2761 ttttggcttt atccacaata cccacggcaa aaccggcgat atcgtaatgg ttttcctggt
     2821 agatgccggg catttcggcg gtttcgccac cgatgagcgc catctccgct tgcatacacc
     2881 cttccacgac gccttcaaca atctgcgcca ttttttcggg cacaagtttg ccagtggcga
     2941 tatagtcaag gaagaagagc ggtttggctc cctggcatag aatgtcgttg gcgcacatgg
     3001 cgaccgcgtc aataccgatg gtgtcgtgtt tatccatgcg gaaggcgata tccagcttgg
     3061 tgccaacgcc atccgtgccc gagaccaaga cgggttcctg gtaggctgcc gtgtcgagtt
     3121 taaagagacc accaaagcca cccaaatctg aaatgaccgc gggggagaag gtctttttga
     3181 ccatttcctt tatcaactta acttctctgt agccggcttc tttgtcaacg ccggcgtctt
     3241 tataacttaa ggtcataata tctcctgaat gggataaaca cccgtaaaac aacggtcgca
     3301 atagtcag
//
LOCUS       DAOSFC010000161         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_27182_length_3293_cov_4.883879, whole genome shotgun sequence.
ACCESSION   DAOSFC010000161 DAOSFC010000000
VERSION     DAOSFC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27182_length_3293_cov_4.883879"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PK791_04815"
     CDS             <1..215
                     /locus_tag="PK791_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOH92668.1"
                     /translation="SDDFEGAIRNAISLGGDTDTLAAITGSIAEAYFPVQPEFILSAY
                     KYLDQTLIDIITNWHDFLKTLPKRAN"
     gene            complement(311..1510)
                     /locus_tag="PK791_04820"
     CDS             complement(311..1510)
                     /locus_tag="PK791_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOH92669.1"
                     /translation="MTKETKKQGFATRQIHGYDVRVPGINPLATPIFQTSTFVFDDCA
                     QGAARFAGKEDGFIYSRIGNPNVEQVAKKVAILEGAEAGLALSSGMGAVSTVLLSLLE
                     QGDHIVADLALYGATFTLMQDALPRYGINTTFVDATDLDMVIDAIKPETRMVYFETPT
                     NPNLKVVDIAELVKRVREKDPLILVVIDNTFATPYLQQPLSLGVDVVVHSGTKYLNGH
                     GDVISGFIASNKTIIDRCRDIGLKMVTGSVLGAFEAFLINRGLKTLDIRMEKHCKNAM
                     KIAEWLETQPKVRKVYYPGLKSFEGYEVAKKQMKLPGAIISFELEASKEAAEQFVNSL
                     ELITLAVSLGDAETLIEHPASMTHAGYSKEMLEEAGISETLIRISVGLEDADDIIADL
                     EKHLAAV"
     gene            complement(1596..>3293)
                     /locus_tag="PK791_04825"
     CDS             complement(1596..>3293)
                     /locus_tag="PK791_04825"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOH92670.1"
                     /translation="MLSGILFVACEPLAVPPDKPVIPSELLQLVTPSPEPEASEEILE
                     TAMPSPELTETPTQEPEGDTPKIWRNENLPDRIKNALSKTDEFALTENQAEADYKLNY
                     LKEGGQSEWVFALVAPFKTLTDEVSFAQFEAFWKGSDDFPAKSLVMNKETLEAMKALL
                     GNPVVEVHTVMCFEEFIEKFHGTSDTWAVLDFEHLREFYKVIAIDGQSPVRKDFDPQS
                     YPLTVSIGLEQVNATDALLEEYEALKARLAEGLPATNREANKLTTVVMTGVTAMVRAT
                     AKEMEIKGVLYPGESVRELLREADITHISNEIPFYDQCPEPQWTQENLVFCSNPRYID
                     LLLDIGADVIDLTGDHFQDYGAEAVYQTLDIYQAHNLPYFGGGRNIDEARTPVKFEVN
                     GNKIAFLGCNGKAPGYASASDTNPGAYHCNMDDMVVAVDKLITEGYLPIVTFQHYEYY
                     HWAAEPYLVEDFRRVAEAGAVAVSGSQGHQPHACEFHEDAFIHYGLGNLFFDQLTTYA
                     DTDKAFIDRLVFYDGRLISVELITTRFYDWSKPVIVYGEDRIEMLEKLFEYSDWEEK"
BASE COUNT          879 a          817 c          706 g          891 t
ORIGIN      
        1 atagcgatga ttttgaaggc gccattcgca atgccatttc cttggggggc gacactgaca
       61 cactcgctgc tatcactggc tcaatagccg aagcgtactt cccagtacaa cctgaattca
      121 ttctgtcagc ctataaatat ctcgaccaaa cgctcattga catcatcact aactggcatg
      181 acttcctaaa aaccctaccc aaacgggcaa actgagataa atcttgaaca tttctaccac
      241 accgagttaa aaataaatta attaaaaaag ccggctgttg aaccggcttg tttattttat
      301 tattgatttc ctagactgca gccaggtgct tttcaagatc agcgatgata tcatccgcgt
      361 cttcaagtcc aactgaaatg cgaattagag tctcgctgat gcctgcttct tcaagcattt
      421 ccttgctata gccggcgtgg gtcattgaag cgggatgctc aatcagcgtt tcagcatcac
      481 caagactaac cgcgagcgta atcagttcaa gtgaattgac aaactgctcg gctgcttcct
      541 tgctggcttc cagttcaaac gagataatcg cgccaggcag tttcatttgc tttttggcaa
      601 cttcatagcc ttcaaagctc tttaagccag gataatagac cttgcgcact ttgggttggg
      661 tctcaagcca ttccgcgatt ttcatcgcgt ttttgcagtg cttttccatg cggatatcca
      721 aagtcttgag tccacggttg attaagaaag cttcaaacgc gcccaaaaca gaaccagtaa
      781 ccatctttag gccgatatca cggcagcggt caatgattgt tttatttgag gcgataaaac
      841 cactgataac atcaccgtgc ccgttcagat acttggtgcc cgagtgaaca accacatcaa
      901 cgcccagcga caatggctgt tgcagatatg gtgtggcaaa ggtattgtcg atgacgacca
      961 aaatgagggg atctttttcg cgcacgcgtt tgaccagttc agcaatatcc acaactttca
     1021 ggttggggtt ggtcggtgtt tcgaaataaa ccatgcgggt ttcaggttta atggcgtcga
     1081 tgaccatatc caaatcggtc gcgtccacaa aggtggtgtt aattccatag cgaggcaaag
     1141 cgtcctgcat gagagtaaaa gttgcgccat aaagagccaa atcagcgacg atatgatcgc
     1201 cctgctccaa aaggctcaac aaaactgtgc tcaccgcgcc catgccggag ctaagggcca
     1261 atcctgcttc agcaccttcg agaatggcaa ctttctttgc cacctgctca acgttgggat
     1321 tgccaatgcg ggagtaaata aagccgtctt cttttccagc aaagcgtgcc gctccctgag
     1381 cgcaatcgtc aaacacgaat gtgctggtct ggaaaatggg tgtggccagg ggattgatac
     1441 cggggacacg tacatcgtat ccatgaattt ggcgggttgc aaaaccttgt ttctttgttt
     1501 cttttgtcat aaagtctcct gagtgattac ctttcataaa cgaacattct tttcaatatt
     1561 attctactat gtatttgcgt aaaatggcga attttttatt tctcttccca gtcgctatat
     1621 tcaaacagct tttccaacat ttctatacgg tcttcaccat agacaataac cggcttcgac
     1681 cagtcataaa agcgcgtggt gattaactca acagatataa gtcttccgtc ataaaacacc
     1741 aggcggtcga taaaggcttt atcagtatca gcataagtcg tgagctggtc aaaaaagaga
     1801 ttgcctaaac cgtagtgaat aaacgcgtct tcatgaaact cacaggcatg cggttgatgc
     1861 ccctgactgc cacttacagc aaccgctccg gcttcagcaa cacgacgaaa atcttcaacc
     1921 aggtatggct cagcagccca gtggtaatac tcgtaatgct gaaaggttac aatgggcaga
     1981 tagccttcgg taatgagctt atcaacagcc acaaccatgt cgtccatatt gcaatgataa
     2041 gcacccgggt ttgtatcgct ggcgctggca taaccaggcg ctttgccatt acagcccagg
     2101 aaggcgattt tgtttccatt tacttcaaat tttactggcg ttcgggcttc atcaatattg
     2161 cgcccaccac caaaataagg caaattgtgc gcctgataaa tatcaagcgt ttggtacacc
     2221 gcttccgcgc cataatcctg aaaatgatcg ccggtaaggt caatcacatc cgcgccgata
     2281 tccagcaata aatcgatata tctggggttc gaacaaaaga cgagattctc ctgcgtccat
     2341 tgtggttccg gacattgatc ataaaacggg atttcgttgg agatatgcgt tatatccgcc
     2401 tcgcgcaaaa gctcgcgcac actttcgcca gggtacaaaa cgcccttgat ttccatctct
     2461 ttggcggtgg cgcgcaccat cgcggttaca ccggtcatca cgaccgtggt taacttattt
     2521 gcttcacgat tggtcgcggg caagccttca gccaatctcg cctttagggc ttcatactct
     2581 tccaacaagg catcagtagc gtttacttgt tcaagcccta tggagactgt taagggatag
     2641 gattgcggat caaaatcttt tcttacaggc gattgtccat caatagcgat gaccttataa
     2701 aattctctca agtgttcaaa atccaacacc gcccaggtgt ccgaagtgcc atgaaacttt
     2761 tctataaatt cctcgaagca catcaccgta tgaacttcaa ccaccggatt acccagcagg
     2821 gctttcatag cctctaaagt ttctttattc ataacaagtg atttagcagg aaaatcgtca
     2881 gaacctttcc agaatgcttc aaactgagca aaactaactt cgtcggtgag ggtcttaaat
     2941 ggtgccacca aggcaaaaac ccattcactc tgaccgcctt ctttaaggta attcagttta
     3001 tagtcagctt ccgcttgatt ctcagttaaa gcaaattcat ctgttttaga taacgcattt
     3061 tttattcgat cagggaggtt ctcatttctc cagatttttg gtgtgtcacc ctctggctct
     3121 tgagtgggtg tttctgtcaa ttccggactg ggcatagccg tctcaagaat ctcctcgctt
     3181 gcttccggtt ctggactggg tgtcaccaac tgcaagagct cagatggaat aacaggttta
     3241 tccggtggca cagcaagagg ctcacaggca acaaataaaa tgcctgacag cat
//
LOCUS       DAOSFC010000162         3282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_27328_length_3282_cov_4.194298, whole genome shotgun sequence.
ACCESSION   DAOSFC010000162 DAOSFC010000000
VERSION     DAOSFC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27328_length_3282_cov_4.194298"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PK791_04830"
     CDS             <1..585
                     /locus_tag="PK791_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92671.1"
                     /translation="HGALVAVATNPTALALLTPPGELGADIVIGEGQPLGMPLSYGGP
                     YLGYFAVTEDLLRKISGRLVGQTTDMDGNLAYVLTLTAREQHIRRGKATSNICSNQGL
                     MTLAATIYMTVLGKEGFKEVANLCYQKAHFAADQLSQVDGYQRAFADTPFFHEFVVKS
                     ERPVAEVLEWFANTTSLVVMTLVKTILSWKIILC"
     gene            complement(656..1900)
                     /gene="gcvPA"
                     /locus_tag="PK791_04835"
     CDS             complement(656..1900)
                     /gene="gcvPA"
                     /locus_tag="PK791_04835"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOH92672.1"
                     /translation="MLTSLGIEKIEDLFSVIPEKFRYPALNLPEQLTEMEAAADLESI
                     ASANGSTKELLSFLGAGAYDHYIPAAVDNLLQRGEWYTAYTPYQPEVSQGTLQAIFEF
                     QSLICQLTGMAVCNASHYDGATAAAEAAILADSHFRGKRKKFLVSRAVNPQYRAVMRT
                     YFRPHDDIQILGDDQETGIDADMAKFIELIDKDTALVLVQYPDFFGRVIDYKALIDAA
                     HAHGALVAVATNPTALALLTPPGELGADIVIGEGQPLGMPLSYGGPYLGYFAVTEDLL
                     RKISGRLVGQTTDMDGNLAYVLTLTAREQHIRRGKATSNICSNQGLMTLAATIYMTVL
                     GKEGFKEVANLCYQKAHFAADQLSQVDGYQRAFADTPFFHEFVVKSERPVAEVLEWFA
                     NTTSLVVMTLVKTILSWKIILC"
     gene            complement(1940..2326)
                     /gene="gcvH"
                     /locus_tag="PK791_04840"
     CDS             complement(1940..2326)
                     /gene="gcvH"
                     /locus_tag="PK791_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOH92673.1"
                     /translation="MKYPQELKYLSTDEWVKVEDGIATIGITDYAQDALSDVVYVEFE
                     IDPDDEVSAGDSIGTIESVKAAAEVHFPVSGTVLEVNEEVVDTPEMLNQEPYEGGWLV
                     KVQVSDESELDALMDAAAYEAYCQDR"
     regulatory      complement(2417..2509)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(2579..>3282)
                     /locus_tag="PK791_04845"
     CDS             complement(2579..>3282)
                     /locus_tag="PK791_04845"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOH92674.1"
                     /translation="FVATGYYARVLQNETGQFELLKGLDPNKDQSYMLSRLTQAELSR
                     TKFPLGELTKHNIRAIAKGFGLSVPVNEESQDLCFLQGNNYRDYLKENFGFTETPGPI
                     KLTDGTLLAEHDGLSAYTIGQRKGLPAYKHALFVIEKRKADNTLIVGGADDLGQNTFS
                     IHDINWITGQAPKNQKNTLVKIRYRATPVPCEISLTKDNQAQIQCAVPLRDITPGQFA
                     VFYQEEHVLGGGVIS"
BASE COUNT          782 a          764 c          846 g          890 t
ORIGIN      
        1 catggcgcgc ttgtggcagt ggcaaccaac cccaccgcgt tggcgctttt gaccccacct
       61 ggtgaactcg gcgcggatat cgtcatcggg gaaggacagc cactcggcat gcccttgtct
      121 tatggtggac cttacctggg ctactttgca gtaaccgaag acctcttgcg caaaatctcc
      181 ggtcgcctgg ttggacaaac aaccgatatg gatggcaacc tggcctatgt gctcaccctt
      241 accgcgcgcg aacaacacat tcgacgtgga aaagcgacct caaatatttg cagcaatcaa
      301 ggtttaatga cccttgccgc gaccatctac atgaccgtcc tgggcaaaga aggcttcaaa
      361 gaagttgcca acctgtgcta ccaaaaagca catttcgctg ctgaccagct tagccaggtc
      421 gatggttacc aacgcgcctt cgcagataca cccttcttcc acgagtttgt tgtcaaatcc
      481 gaacgtcctg tagccgaagt gctcgaatgg ttcgcgaaca cgacatcctt ggtggttatg
      541 accttggtca agactatcct gagctggaaa atcatcttat gttagccgta actgaaaaaa
      601 tctcccgcga agacattgaa ttcaatgtct tcgcgggaga ttttttcagt tacggctaac
      661 ataagatgat tttccagctc aggatagtct tgaccaaggt cataaccacc aaggatgtcg
      721 tgttcgcgaa ccattcgagc acttcggcta caggacgttc ggatttgaca acaaactcgt
      781 ggaagaaggg tgtatctgcg aaggcgcgtt ggtaaccatc gacctggcta agctggtcag
      841 cagcgaaatg tgctttttgg tagcacaggt tggcaacttc tttgaagcct tctttgccca
      901 ggacggtcat gtagatggtc gcggcaaggg tcattaaacc ttgattgctg caaatatttg
      961 aggtcgcttt tccacgtcga atgtgttgtt cgcgcgcggt aagggtgagc acataggcca
     1021 ggttgccatc catatcggtt gtttgtccaa ccaggcgacc ggagattttg cgcaagaggt
     1081 cttcggttac tgcaaagtag cccaggtaag gtccaccata agacaagggc atgccgagtg
     1141 gctgtccttc cccgatgacg atatccgcgc cgagttcacc aggtggggtc aaaagcgcca
     1201 acgcggtggg gttggttgcc actgccacaa gcgcgccatg cgcgtgagcg gcatcaatga
     1261 gggctttgta gtcaataacc cgaccgaaaa agtctggata ctgcaccagg acgagcgcgg
     1321 tatctttgtc aatcagttcg ataaactttg ccatgtcagc atcgatgccg gtttcctgat
     1381 catcgcccag gatttgaatg tcatcatgcg gacggaaata agtgcgcatc acggcacggt
     1441 attgaggatt aaccgcgcgg gaaacgagga actttttgcg ttttcctcta aagtgactgt
     1501 cagccaggat ggcggcttca gcggcagctg tggcgccatc atagtgggaa gcgttacaga
     1561 ctgccatgcc ggtgagttgg caaatgagac tttggaattc aaaaatcgcc tgcagggttc
     1621 cctgagaaac ttcaggctga tagggggtat aagcggtgta ccactcgcca cgttgcaaaa
     1681 ggttgtcaac cgcggctgga atgtagtggt cataagcacc cgcacccagg aaactcaaaa
     1741 gctccttggt gctgccattg gcggaggcga tactctctaa atctgccgca gcttccattt
     1801 cggttagctg ttcggggaga ttgagtgcag gatagcgaaa cttttcgggg atcactgaga
     1861 agagatcctc gattttttca atacctaatg atgtaagcat cacctccaca tctttttgtg
     1921 tgtggggtgt aaacatagat tatcggtcct ggcagtatgc ttcgtaggcg gcggcatcca
     1981 tgagggcatc cagttcactt tcatctgaaa cctggacttt taccagccaa ccaccttcgt
     2041 aaggttcctg gttgagcatt tcgggagtgt caacgacttc ttcgttaact tccaacacag
     2101 ttccactgac agggaagtgg acttcagcag cggcttttac ggattcgatg gtgccgatgc
     2161 tgtcgccggc gctgacttcg tcgtcggggt caatttcgaa ttcaacgtaa acgacgtcgg
     2221 aaagggcgtc ttgcgcgtag tcagtgatgc cgatggtggc aataccgtct tcaaccttga
     2281 cccattcgtc agtacttaga tatttgagtt cttgtggata tttcattgtg tctcctttaa
     2341 tcaatgtttg tttttaaatt tgtaaagtat attaaaacag gagcacatgg tgtgctcctg
     2401 ttgtcaattg acttcagaag ctcgttgggt cttggtacct gagagtttca cccgtgtggg
     2461 cttgcccctt cggtgcccac cttatgtggg tctcttcccg ttgagctggt aagtttattt
     2521 tagcaatttg gtggctaaag tcaagcattt tcagtatatg ttgttattga tgaataggct
     2581 atgagatgac tccaccaccc aaaacatgtt cctcctgata gaaaacagcg aattgacctg
     2641 gcgttatatc acgtaaaggt acagcgcatt ggatttgcgc ttgattatct ttagttaagc
     2701 tgatttcgca aggtacaggt gtggcgcgat atcggatttt gacgagagtg tttttctgat
     2761 tttttggtgc ctggccagtt atccaattga tatcgtgaat ggaaaaagtg ttttgtccaa
     2821 gatcatccgc acctccaaca atgagggtgt tatcggcttt gcgtttttcg ataacaaaca
     2881 aggcatgttt gtaagctggt aagcctttgc gttgaccgat ggtgtaggca ctcaatccat
     2941 catgttcagc taacaatgtg ccatcagtga gtttgatggg accgggcgtt tctgtaaaac
     3001 caaagttctc ttttaggtaa tcacgataat tattgccttg taaaaaacaa agatcctgac
     3061 tttcttcgtt cactggcacg gataagccaa aacctttggc gattgcgcgt atattatgct
     3121 tggttagttc gcctaagggg aatttcgtcc gtgaaagttc ggcttgggtc aggcggctaa
     3181 gcatataact ttgatctttg ttggggtcga gtcctttgag taattcaaat tgaccggttt
     3241 cgttttgcaa gacacgcgcg taataaccag tggcaacaaa gt
//
LOCUS       DAOSFC010000163         3274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_27423_length_3274_cov_6.602050, whole genome shotgun sequence.
ACCESSION   DAOSFC010000163 DAOSFC010000000
VERSION     DAOSFC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27423_length_3274_cov_6.602050"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PK791_04850"
     CDS             complement(<1..776)
                     /locus_tag="PK791_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOH92675.1"
                     /translation="MKQKNNHLNDQDHDDVASAKADSSEQTLESMVEQSEQASKAGRQ
                     IARAASVVMLAYVFSTLIGILRGMVVSGAFGTSADLDSFNAANRVTELLFNLTAGGAL
                     GSAFIPMFTGMLTKREENKAWRLASGVMNTLVIILIIVAALMWVFAPAIVEKGLFVLT
                     PGTQVTQIELTVRLLRIMLPTVIIFGVSGLIMGILNAHQVFLWPALAPSAYSLGIILG
                     VLLLPKSMGIDRLAYGTLIGAAGHFLLQAPTLFKLPKRAY"
     gene            complement(805..2259)
                     /gene="mazG"
                     /locus_tag="PK791_04855"
     CDS             complement(805..2259)
                     /gene="mazG"
                     /locus_tag="PK791_04855"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOH92676.1"
                     /translation="MQKQGIVIVGLGPAGANYLTREAWDHLLSCEEIYLRTAFHPVCS
                     ELPENLSRHSFDHLYESHDDLEGVLDAIVTELVALAGRPQGVTYGVPGSPFIGEETVS
                     QVVKMLAGTDIPVRIIDGLSYLEPVSSALGLDLLPQTAILDGLYVGSLLTPPFPPSVP
                     AIISQLGGQFEASELKLTLMHNYPDEFEVKLLHNLGTSEEIIETLPLYAIDQSVHLGM
                     MSTLYLPAREESTAFEDLQEVIARLRAPGGCPWDREQTHLSLRQHLLEEAYETLEALD
                     DEDMDSLCEELGDLLLQIVLHAQIATEEGDFNMTDVLSGINNKLKRRHPHVFAGLDVD
                     SVDTVLHNWEKIKASERKDHQERPQGLLDSVPKIAPALSQSEQIQRKAARVGFDWQTI
                     EPVVDKIHEELAELKAADSPEEREKEGGDVLFAMVNLLRWLEVDPENALRGTNARFKS
                     RFAYIEDSARKQGKSLSELSFEEMDALWDEAKLQ"
     gene            complement(2398..2790)
                     /gene="rpsI"
                     /locus_tag="PK791_04860"
     CDS             complement(2398..2790)
                     /gene="rpsI"
                     /locus_tag="PK791_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOH92677.1"
                     /translation="MAQYIEAVGRRKQSTARVRIYEGSGVITVNDKPIADYFPRGGDI
                     PAILEAIEATGKNPNAYDVTVKVMGGGVSGQRDAVKLGLARALLAEDESVRTALRDKG
                     LLTRDARVKERKKPGLKRARKAPTYTKR"
     gene            complement(2804..3232)
                     /gene="rplM"
                     /locus_tag="PK791_04865"
     CDS             complement(2804..3232)
                     /gene="rplM"
                     /locus_tag="PK791_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOH92678.1"
                     /translation="MDKTYVPKGKPEGEWYLVDASGQTLGRLATKIAEILVGKHRPDF
                     TPGVVLGDHVVVVNAEKIYANPTRTSQKLYRRHTGYPSGLREVTYERMMERHPDRIIR
                     FAVRGMLPQNKLTDRYLANLKIYAGPEHPHEAQGPKPFGE"
BASE COUNT          813 a          878 c          786 g          797 t
ORIGIN      
        1 aaatacgcgc gcttggggag tttaaacaat gttggcgctt gcagtaaaaa gtgaccagcc
       61 gcgccaatca gtgttccgta tgccaggcga tcaatgccca ttgattttgg caagagtaat
      121 acgcccagaa tgatgcccaa agaataggcc gatggcgcca gcgccggcca caaaaagacc
      181 tgatgcgcgt tgagaatgcc cataatcagt ccactcacgc cgaaaataat caccgtgggt
      241 aacatgatgc gcaataatcg caccgtgagt tcaatttgcg tcacttgagt gccaggtgtg
      301 agcacaaaaa gccctttttc cacaatagct ggtgcaaaga cccacatgag tgccgccaca
      361 ataatcaaaa tgataacgag cgtattcata acacccgacg ccaaacgcca ggctttgttc
      421 tcttcacgct tggtcagcat tcccgtgaac atgggaataa acgccgagcc caaggcaccc
      481 ccggcggtaa ggttgaaaag cagttcggta acacggttgg cggcattgaa agaatcaagg
      541 tcagctgatg taccaaacgc gcccgaaaca accatgccac gcaagatgcc aatgagcgtg
      601 ctaaaaacat aggccagcat aaccacagat gccgcgcggg cgatttgtcg cccggctttg
      661 ctggcttgtt cagactgttc caccatcgac tcaagcgttt gttcgcttga atcagcttta
      721 gcagacgcca cgtcatcgtg gtcctggtca ttaagatgat tatttttctg cttcatcaag
      781 tatcaccacc aataactgct gtctttattg caacttggcc tcgtcccaca gagcatccat
      841 ttcttcaaaa ctcagctcac tgagactttt accctgtttg cgtgctgagt cttcgatata
      901 cgcgaaacgg cttttaaagc gcgcgtttgt accacgaagc gcgttttccg ggtcaacttc
      961 gagccagcgg agaaggttca ccatggcgaa caaaacgtcg ccaccttctt tttcgcgctc
     1021 ttcgggacta tcggctgctt tgagttccgc gagttcttca tgtattttat caacaactgg
     1081 ctcaatcgtc tgccaatcaa agccaacccg ggcagctttg cgctgaattt gctccgactg
     1141 actcaaggcc ggcgcgattt taggaacgct gtccaacaag ccttgcggac gttcctgatg
     1201 gtctttgcgt tcgctggctt ttatcttttc ccagttatgc agcacggtat caaccgaatc
     1261 aacatccaag cccgcaaaaa catgcggatg gcggcgttta agcttgttgt tgataccact
     1321 aagcacatcg gtcatattaa agtcgccctc ttcggtcgcg atttgggcat gcaagacaat
     1381 ttgcaacaac aaatcgccca gttcttcaca caaactgtcc atgtcctcat catcaagggc
     1441 ttccagcgtt tcataggctt cttcaagcaa atgctggcgc aagctaagat gagtttgctc
     1501 acggtcccac gggcagccac ctggcgcgcg caagcgggcg atgacctctt gcaggtcttc
     1561 aaacgcggtg gattcctcgc gcgcgggcag ataaagcgta ctcatcatgc ccaaatgcac
     1621 ggactggtcg atggcataaa gcggtaaggt ttcaataatt tcttcggacg ttccaagatt
     1681 atgaagtaat ttaacttcaa attcgtccgg ataattatgc atcagggtta gcttgagctc
     1741 tgaagcttca aactgaccgc ctaactggct gataatcgcc ggcacggatg gcgggaaagg
     1801 tggcgtgagc aaacttccca cgtaaagccc atccaaaatc gctgtctgag gcaaaaggtc
     1861 cagacccagt gcggaactga ccggttccaa atagctcaag ccgtcaatga tgcgcacagg
     1921 gatgtccgtt cctgccagca ttttaacgac ctggctcacg gtttcttcac cgataaaagg
     1981 actgcctggc acaccataag tcacgccctg cggtcgcccg gcaagcgcga caagttcggt
     2041 aacaatggca tccaaaacac cttcaagatc gtcgtggctt tcatacagat ggtcaaaact
     2101 atgacgcgag agattttccg gaagttctga acaaaccgga tgaaaagccg tgcgcaagta
     2161 aatttcttca caactcaaaa gatggtccca ggcttcgcgc gtgagataat tcgcgccagc
     2221 ggggcctagt ccaacaatga cgataccttg tttttgcatc gcattctccc tggcaaaaag
     2281 cattgccgat ttgaattaag caaaggctgt ccacccaaac ggtggcagcc tttgtaatgt
     2341 aaataagttg cgacaggcat atacttcagt aagtatacct gcccgacaac gagcaaacta
     2401 acgcttggtg taggtaggag ccttgcgagc gcgtttgaga ccgggtttct tgcgttcctt
     2461 aacgcgagcg tcgcgggtaa gcagaccctt atcgcgcaaa gccgtacgca ccgattcgtc
     2521 ttcagcaagc aaagcgcgcg ccaaaccaag tttcacggca tcccgctgac cgctcacacc
     2581 accacccatg acctttacag tcacgtcgta ggcgttgggg ttctttccgg tagcttcaat
     2641 cgcttccaaa atagcgggga tgtcgccacc acgggggaaa tagtccgcga tgggtttatc
     2701 gttgacggta atcacgccac tgccttcgta aatgcgcacg cgggcagtac tttgtttgcg
     2761 tcgtccaaca gcttcgatgt attgtgccat aattcctctt tatctactct ccgaatggtt
     2821 tcggaccctg ggcttcgtgg gggtgttcgg ggccagcata aatcttgaga ttagcaagat
     2881 agcggtcagt gagcttattt tggggcaaca tgccacgcac cgcaaagcgg atgatgcggt
     2941 cgggatgtcg ttccatcata cgttcataag tcacttctct gaggccactt ggataaccag
     3001 tatgtcgtcg atacaatttt tgggacgtac gggttggatt ggcatagatt ttttcagcgt
     3061 taaccacaac cacgtgatca cccaaaacca caccgggtgt gaaatcgggt cgatgtttgc
     3121 ccaccaaaat ttcagcgatt ttggttgcca agcgtccgag cgtttgacca ctggcgtcta
     3181 ccagatacca ctcgccttca ggctttcctt tagggacata agttttatcc actttttctt
     3241 tctccgtttt ctactttcac cggttttaag ccgg
//
LOCUS       DAOSFC010000164         3242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_27821_length_3242_cov_4.138375, whole genome shotgun sequence.
ACCESSION   DAOSFC010000164 DAOSFC010000000
VERSION     DAOSFC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27821_length_3242_cov_4.138375"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="PK791_04870"
     CDS             <1..567
                     /locus_tag="PK791_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HOH92679.1"
                     /translation="EKRIVAYHEIGHALVAAKQSGSAPVQKITIVPRTSGALGFTMQV
                     EENEKSLYSKKDAYQKLEVLCGGRAAEELVFDIVTTGAANDIERATELARNMVTRYGM
                     SDNFGMMALQTGGNTYLGEPGRSTCSPETARLVDEEIRDIIAKAHKNATDILRENREK
                     MDELAEYLIEKETITGKEFMEILEGETA"
     gene            complement(610..978)
                     /gene="acpS"
                     /locus_tag="PK791_04875"
     CDS             complement(610..978)
                     /gene="acpS"
                     /locus_tag="PK791_04875"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOH92680.1"
                     /translation="MIKCGVDIVRVNRLAEINPKIRARFIERVFTEREQAQMHDNNEG
                     LSGLFAVKEAVSKALGTGIGKVAWRDIEIVHLPTGEPTLTLHGYAKVIAHSKGLEEWA
                     ISISHDGGLAIAMVVATGKS"
     gene            complement(975..2255)
                     /locus_tag="PK791_04880"
     CDS             complement(975..2255)
                     /locus_tag="PK791_04880"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOH92681.1"
                     /translation="MTQMSQNLPHAGNILRTSFNNSSTLLAWHNSTSPAVVIQGLLPP
                     GTVADTPGQYGLANLTTAMLMTGTQKHDFQALHEKIESLGASIHVQSGKLMTSFALQC
                     LAEDLPTILDLLTEILSQPAFPEKHFARIQSQILTTLAIRAQDTAAMADQAFDRLLYQ
                     THPFAHPSIGYAQDMMALTLDDLRAFHSDYYTPQNMIIAASGGLAPEELTKIVENTLG
                     AWHKTAPQKQPDLPDFFPPTKSQREHITMEDKSQTDLIIGVLGPKTQGDDFQAASIAN
                     NILGQFGMMGRVGESVREKAGLAYYVQSALGAGLGPTPWQVVAGVNPSNLDKAIALIK
                     EELHKFISEPVTTKELEDSRAQAIGRLPLALESNTGITRSLLSMERYQLGLNHLRDLP
                     AKLAAITAQNILTVARRYWNLDTLIISSAGKEIA"
     gene            complement(2259..>3242)
                     /locus_tag="PK791_04885"
     CDS             complement(2259..>3242)
                     /locus_tag="PK791_04885"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOH92682.1"
                     /translation="ALKIEADRMQNSLFDPDDVEMERTVILSEREGAENDPLFLLGEA
                     TEALAFQTHPYRNEVLGFQADIESLTRDDLYGHYKNYYQAQNARLCLAGDFDTPTALK
                     MIEDYFGAIPSHPVKKNIPTPEGRITQAHHIELEGPGDAHFAEIVYRSPAANTDDFFA
                     YTILDSLLAGPSSLNMFGSGGISNRTSRLYKALVENEIVAGVFAGINATIDPGSYSFT
                     LICRPDRASEDVLKRFDEQIEDIRNGNIQKTELERAVKQAKALFVYGADNITNQGFWL
                     GYAPSFADYNWFLQYIERLQAVTPEQVIAIAQKYFSPDERVVGFYNPIKKD"
BASE COUNT          784 a          688 c          925 g          845 t
ORIGIN      
        1 gaaaaacgca ttgtggctta tcacgaaatc ggacacgcgc ttgttgcagc aaaacaatcc
       61 ggttcagcac cggtacaaaa aattacgatt gttcccagga cttccggcgc attagggttt
      121 accatgcaag ttgaagaaaa tgagaaaagt ctgtacagca aaaaggatgc ttatcaaaaa
      181 ctggaggtct tatgcggtgg cagagcggcc gaagagcttg tctttgatat cgtgactaca
      241 ggcgctgcta atgatatcga aagagctaca gaactggcaa gaaatatggt gacccgttac
      301 ggtatgtccg ataacttcgg aatgatggca ctgcaaacgg gaggaaatac ttacctggga
      361 gagccgggtc gatctacgtg ttcgccggaa accgcacgcc tggtagatga ggaaatcagg
      421 gatatcatag cgaaagccca taaaaatgcg acagatatcc ttagagagaa cagagaaaag
      481 atggatgaat tggctgaata tttaattgaa aaagaaacta ttaccggcaa agaatttatg
      541 gaaattctcg agggagaaac agcctaatca ttaaatcatt ttgatatatg accttgcttc
      601 aaagtgggtt tagcttttgc cagtggcaac gaccattgcg attgccagac cgccatcatg
      661 actgatggat atcgcccatt cttcaagtcc tttggaatgg gcgattactt ttgcgtagcc
      721 gtggagtgtc agggttggct cgccagtagg gaggtgtaca atttcgatgt cacgccaggc
      781 gactttgccg atgccggtgc ccagggcttt ggaaacagct tcttttacag cgaacaatcc
      841 actaagacct tcattgttat catgcatttg agcttgttcg cgttcagtga aaacacgctc
      901 aataaaacgc gcgcggattt tggggttaat ctctgccagg cgattgacgc gcacgatatc
      961 gacaccacat ttaatcatgc gatttccttt cctgctgaac tgatgatgag cgtgtcaaga
     1021 ttccagtagc gtcgcgccac ggtgagaatg ttctgggcag tgatggcagc gagtttggcg
     1081 ggcaaatcgc gcaggtggtt aagccctagc tgatagcgtt ccatgctgag caaagagcgc
     1141 gtaatgccgg tgttactttc gagcgcgaga ggcaagcgac cgatggcttg agcgcgactg
     1201 tcttccagtt cttttgttgt aaccggctcg ctaatgaatt tatggagttc ttctttgatt
     1261 agcgcgatgg ctttatccag attgctgggg ttgacgccag ccacaacctg ccatggggta
     1321 gggccaagac cagcacccag ggcgctttgc acgtaatagg caaggccggc tttttcgcgc
     1381 actgattcgc caaccctgcc catcatgcca aattgaccaa gaatgttgtt agcgattgag
     1441 gctgcctgga aatcgtcgcc ttgtgttttg gggccaagaa ccccgatgat taaatcagtt
     1501 tggcttttgt cttccatcgt gatgtgttcg cgttggctct ttgttggcgg gaaaaagtca
     1561 ggaagatcag gttgtttttg gggtgctgtt ttatgccatg cgcccaaagt gttttcgacg
     1621 attttcgtca gttcttcagg tgctaaaccg cccgaagcag cgataatcat gttttggggt
     1681 gtgtaataat cgctgtgaaa agcgcgtaag tcgtccagag taagtgccat catatcttgc
     1741 gcatagccta tagacgggtg agcgaaggga tgggtctggt agagcagacg gtcaaaggcc
     1801 tggtcggcca tagcggcggt gtcctgggcg cgaatagcca gggtggtcaa aatttggctt
     1861 tggatgcgcg cgaagtgttt ttcggggaat gccggctggc tgaggatttc ggtaagcagg
     1921 tccagaatgg tgggcaggtc ttctgctaag cattgcaggg caaagcttgt catcagtttg
     1981 ccggattgta cgtgaatgga cgcgcctagg ctttcaatct tttcatgaag agcttgaaag
     2041 tcatgctttt gtgtgccggt catcaacatg gcggtggtaa ggttagcaag accatattga
     2101 ccaggtgtat ctgcgacagt acccggtggg agcaaacctt gaataaccac agcggggctg
     2161 gtgctgttgt gccaggcgag cagggtggaa ctgttattaa agcttgtgcg gaggatattg
     2221 ccagcgtggg gcaggttttg agacatttgt gtcatggctt aatctttctt gatggggtta
     2281 taaaagccca ccacgcgttc atcgggagag aagtattttt gggcgatggc aatgacctgt
     2341 tcaggtgtaa cagcttgcag acgttcaatg tattgcagga accagttata gtcagcaaaa
     2401 cttggggcat aacccagcca aaaaccctgg ttggtgatgt tatccgcgcc gtaaacgaaa
     2461 agggctttag cttgtttcac cgcgcgttcc agctccgttt tttggatatt gccattgcgg
     2521 atatcttcga tttgctcatc gaaacgcttt agaacatctt ctgatgcacg gtcgggacga
     2581 caaatgagcg tgaaagaata actgcccggg tcaatggtgg cgttaatgcc ggcaaataca
     2641 ccggccacaa tttcgttttc aacgagtgct ttgtataagc gactggttcg gttgctgatg
     2701 ccaccactgc caaacatgtt gagtgaactg ggtccggcca ggagactatc caggatggtg
     2761 taggcgaaaa agtcatctgt gttggctgct ggtgaacggt agacaatttc agcaaaatgc
     2821 gcgtcaccgg ggccttcgag ttcgatgtga tgggcttggg tgatgcggcc ttccggtgtg
     2881 ggaatgtttt tcttgactgg atgcgaagga atagcgccaa aatagtcttc gatcattttc
     2941 agggctgttg gcgtgtcaaa atcacccgcc aggcaaaggc gcgcgttttg ggcttggtag
     3001 tagtttttgt aatgaccgta aaggtcatcg cgggtgagac tttcgatatc tgcctgaaac
     3061 ccaagtactt cgttgcgata ggggtgggtt tgaaaagcga gggcttcagt ggcttcgccg
     3121 agcaggaaaa gcgggtcgtt ttctgcgcct tcgcgttcgg ataaaatgac ggtgcgttcc
     3181 atttcaacgt catcggggtc aaacaagctg ttttgcatgc ggtcggcttc aattttaagc
     3241 gc
//
LOCUS       DAOSFC010000165         3232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_27956_length_3232_cov_4.711992, whole genome shotgun sequence.
ACCESSION   DAOSFC010000165 DAOSFC010000000
VERSION     DAOSFC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27956_length_3232_cov_4.711992"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /gene="meaB"
                     /locus_tag="PK791_04890"
     CDS             complement(<1..967)
                     /gene="meaB"
                     /locus_tag="PK791_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOH92683.1"
                     /translation="MPAELTSELRKGNRLALSRVLSQIEKGSEKSVRILESLFPFTGQ
                     AHKIGITGPPGSGKSTLVNALARQLRTLESHPKVAIIAVDPTSPFSGGAILGDRVRMQ
                     DLQGDKGIFIRSMASRGALGGLAAQTEMMSQAFDAAAYEYIIIETVGAGQSEVDIVNL
                     AHTTIVVDVPGMGDDIQSIKAGILEIADILVVNKSDQLGADQSVKHLQNMIAMGYRGL
                     RYAGGKHRLSSAMPQKQDEIETSGWVPPVLKTVAFDGTGVDKLADEIVKHGKYLKETG
                     LWTQKAESAITDTLRKLIQSTLYQQWRQTIPDEELEDIIIQVLQRT"
     gene            complement(970..1374)
                     /locus_tag="PK791_04895"
     CDS             complement(970..1374)
                     /locus_tag="PK791_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOH92684.1"
                     /translation="MPEKKIRVLVAKPGLDGHDRGAKVIARALRDDGMEVIYTGLRQT
                     PEMIAEAALQEDVDVVGLSILSGAHNALVPQIIQELKDNGQENIVVFLGGIIPEDDIQ
                     GLLEQGVHKVYGPGTDTREVCKDIRSAVSAEA"
     gene            complement(1433..1960)
                     /locus_tag="PK791_04900"
     CDS             complement(1433..1960)
                     /locus_tag="PK791_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92685.1"
                     /translation="MNNLIQIDPKSFVLKPHYLFNNQWFLLSAGNFNKNDFNCMTIGW
                     GSIGTMWNKPYVSVVVRPTRHTFHFMEKYPDFTLTAFPDAFKKDLAYLGSHSGRDEDK
                     LAKTQLTPIKAQQLTAPNYAQAELCIECRQIYSADFLPSKFKSPEIETHYPKKDYHRV
                     YYGEIICIQGDIKYC"
     gene            2045..3046
                     /locus_tag="PK791_04905"
     CDS             2045..3046
                     /locus_tag="PK791_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOH92686.1"
                     /translation="MQNEQKNKVKLIIRWAGTIVSIAIMVYLLSKVGWREAWETVSQL
                     AWWRIGVILVLVFVSRFATYFRWQALLSVQDEKVSAVEVLKLTFAGLFASNFMPTTIG
                     GDVFRLAGAIRLGIDSALATASLIADRIVGMTGMTLVLPLAIPNFVAYIKENQKPQET
                     IRYIPLLMNVELNDDESDKKKPPIFKRLIQKLKNGLKKLRESFAYWLKNPKSLIKALG
                     FTLVHQLAIYLIITVLINGMGESLPFYQVAGIWSLTYFITLLPVSVNGLGLAEVTITN
                     LYSILGGISPATSIALALILRIVWMIGSLPGAFFIGDVLAGKEKEIKSEGDDGLSRD"
     gene            3027..>3232
                     /locus_tag="PK791_04910"
     CDS             3027..>3232
                     /locus_tag="PK791_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92687.1"
                     /translation="MDYQEIKPFVRQWLLLLGGTVAWYLYQSIHNRNTAFFVIAFIGL
                     LVLAVLLAVVFINLKKPVILNLNIS"
BASE COUNT          787 a          667 c          812 g          966 t
ORIGIN      
        1 aagttctttg taaaacctga atgataatgt cttccagttc ttcgtctggt atcgtctggc
       61 gccattgttg ataaagtgtg ctttgaatga gtttgcgtaa agtgtcggtt atggctgact
      121 cggctttttg ggtccataga ccagtttcct tgagatactt gccatgtttg acaatttcat
      181 cagcaagttt gtcaacacca gtaccatcaa aggcaaccgt ttttaagaca ggtggaaccc
      241 aaccacttgt ttcaatctca tcttgtttct ggggcatggc tgatgaaaga cgatgcttgc
      301 caccagcgta gcgcaagccg cgataaccca tagctatcat attttgcaga tgcttaacac
      361 tctgatctgc gccaagttgg tcggatttgt tgacaaccag gatatcggcg atttcaagga
      421 tgccggcttt gatgctttgg atatcatcac ccatgccggg cacgtcgacc acgatggtgg
      481 tatgagccag gttgacgata tctacttcgc tttgacccgc gccgacagtt tcgatgatga
      541 tgtattcata agcagctgcg tcaaatgcct ggctcatcat ctcagtttgg gcggcaagtc
      601 cgcctaatgc gccacgagat gccattgaac gaataaagat gcctttatcg ccttgcaggt
      661 cttgcatccg cacgcggtcg cctaaaatag cgccaccgga aaagggactg gtgggatcaa
      721 ccgcgatgat ggcaactttt ggatggcttt ccagcgtcct aagctggcga gccagggcgt
      781 ttaccagggt gcttttgcca gaaccgggtg ggccggtaat accgattttg tgcgcctgcc
      841 ccgtgaaagg gaagagggat tcaagaattc ggacgctttt ctcggaccct ttttcaattt
      901 ggcttaaaac gcgagaaagg gcaagcctgt tgccctttct gagttcactc gttagctcag
      961 cgggcatgcc tatgcctcgg ctgaaacggc actgcgaatg tctttgcaga cttcgcgggt
     1021 gtcggtgccg ggtccgtaga ctttgtgaac gccttgttca agaagtccct ggatatcgtc
     1081 ttcgggaatg atgccaccca aaaagacgac gatattttcc tgaccattat ccttgagttc
     1141 ctgaatgatt tggggaacca gggcgttgtg cgcgccacta aggatagaaa gaccgacgac
     1201 gtcaacgtct tcttgcaggg cggcttcggc aatcatttcg ggcgtttggc gcaggccggt
     1261 atagatgact tccataccgt catctctgag cgcgcgggcg ataactttcg caccacggtc
     1321 gtggccatcc aagccaggtt tggcaactaa tacacgaatt tttttctcag gcatttagat
     1381 cctccaaatg ttatgctttt attataatgc aagaatctgg cttttggaga atctagcaat
     1441 actttatatc gccttgaatg cagataatct cgccataata aacgcggtga tagtcctttt
     1501 tagggtaatg ggtttcaatc tcggggcttt taaattttga tggaagaaaa tcagcactgt
     1561 agatttggcg acattcgatg cataattccg cctgggcata gtttggggcg gtaagctgct
     1621 gggcttttat aggtgtcagt tgtgttttgg cgagcttgtc ttcatccctg cctgagtggc
     1681 tgcctaagta tgccaggtct tttttgaaag catccgggaa ggcggtcaat gtgaaatcag
     1741 ggtatttctc catgaagtga aaggtgtggc gcgttgggcg tactaccacg gatacatagg
     1801 gtttattcca catggtgccg atacttcccc aaccgatggt catacagttg aagtcgtttt
     1861 tgttgaaatt ccctgcgcta agcaggaacc attgattatt aaacaaataa tgtggtttga
     1921 gaacgaacga ttttgggtca atttgaataa gattgttcat ggagtctcct tgcctggcta
     1981 atgaaaatta tttagttata gaaacaagta taaccaatct tgcttagcgg gtataattct
     2041 gattatgcaa aatgaacaaa agaacaaagt aaaactcatc attagatggg ctggtacgat
     2101 agtttcaata gcgattatgg tttatttgct ttcaaaagta ggttggaggg aagcatggga
     2161 gacggttagc cagttggcat ggtggcgaat tggcgtgatt ttggtcttgg tttttgtttc
     2221 gcgttttgcc acttacttta gatggcaggc tttactgtcg gtgcaagacg agaaagtttc
     2281 agctgttgaa gtattgaagc tgacttttgc gggcttgttt gcgtcaaact ttatgcctac
     2341 tacaataggt ggcgatgtct tccgcttggc tggcgcaata cgtttgggga ttgacagcgc
     2401 gttggcaact gcctcgctga ttgcggaccg tatcgttggc atgacaggca tgaccctggt
     2461 tttacctttg gctattccaa actttgttgc ttacattaag gaaaaccaga agccacagga
     2521 aacaatacga tatattccct tgttgatgaa tgttgagctg aatgatgatg aatctgacaa
     2581 gaaaaagcct ccgatcttca aacgcttgat acaaaaactc aaaaatgggc tgaagaagtt
     2641 aagagaaagc tttgcctatt ggttaaagaa tccgaaatct ttgataaaag cgctgggatt
     2701 tacgcttgtt catcagctgg ctatttattt gattattaca gtgctcatca atggcatggg
     2761 ggaaagctta ccgttttacc aagtggctgg catttggagt ttgacgtact ttatcacttt
     2821 gttgcctgtt tcggtgaatg gcttgggttt ggcagaagtg accatcacta atctatatag
     2881 cattttaggc ggtatcagtc ccgcgacaag tattgctctg gcgcttatcc tgcgtatcgt
     2941 gtggatgatt ggcagtttac cgggcgcttt tttcatcggc gatgttttag ctggaaaaga
     3001 gaaagaaatc aaatcagaag gtgatgatgg attatcaaga gattaaaccc tttgttcgtc
     3061 aatggttatt gctattgggt gggacagtcg cctggtatct ttaccaaagt attcataacc
     3121 gaaacactgc attttttgtc atcgcgttta ttggcttgct tgtcttagcg gtgcttttgg
     3181 cagtcgtttt cattaatctc aaaaaacctg tcatcttaaa cttgaatatt tc
//
LOCUS       DAOSFC010000166         3219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_28111_length_3219_cov_6.814159, whole genome shotgun sequence.
ACCESSION   DAOSFC010000166 DAOSFC010000000
VERSION     DAOSFC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28111_length_3219_cov_6.814159"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2475)
                     /locus_tag="PK791_04915"
     CDS             complement(1..2475)
                     /locus_tag="PK791_04915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH92688.1"
                     /translation="MKIVIVGGVAGGATAMARLRRLDENAEIVLFERGKYISFANCGL
                     PYYVGGAIASRDALFVSTKEKFEKDYGVDIRVESEVIRIDPANKTVDVYDRANEKHYT
                     ETYDKLLLSTGSRPFVPKVPGTDNPNVFQLWTVPDVDKIVQFIQEKQPQNAVVVGGGF
                     IGLEMAENLAHRGLKVTVVEMMNQVMPPLDADMAKLVENHMAENGITLKLGKAFSGMS
                     EDGQKVMLSDGQALEADMVVLSIGVRPNNELAKEAGLSLSERGGILVDEYMQTSDPDI
                     YAVGDVISVQDYVMGTPTMIPLAGPANKQGRAVAANILGKKPETYVGTMGTSILRLFD
                     ITAASVGANEKTLNKAGQKYGQDYFIALSHPKCHSGYFPGALPLSVKVIFDKEGKVLG
                     GQIVGYEGVDKRIDVLASVIYFKGTVKDLSQLELAYAPPYSSAKDPLNFAGYMGMNIL
                     EGLTDPIFYHEWAQNPDAYTLLDIREPDETIAGSIPGALLIPHSQLRERISELDKSKT
                     YAILCRVGRRGFISERLLKQHGFKVKNFAGGLQSYHDMEYDITMNTNTTPDTKASNIP
                     AEIAPVASTVGKLETLDVCGLSCPGPIVQVANTLKTLNDGDQLDVTASDPGFTRDIAA
                     WCKNTGNTLIKSESEKGIYRAIVQKGLSAGAGTLPQTVAQASAPLKEKTMIIFDNDLD
                     KAIAAFIIANGAAAMGNKVHMFFTFWGLSILRKEQYVPVKKDFMGKMFGMMLPKGSRK
                     LGLSQMNMAGAGAKMIRGIMKHKGIDSLEQLIQQAIDAGVELVACQMTMDIMGLKQEE
                     LLDGVQIGGVATMLNDSDNSNMNLFI"
     gene            complement(2489..2806)
                     /locus_tag="PK791_04920"
     CDS             complement(2489..2806)
                     /locus_tag="PK791_04920"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH92689.1"
                     /translation="MKQNDLPLSENETHRKELEAKAEILKALANPVRLCLMEQIIKRG
                     ETTVSELVTCMGASQSLISQYLGRLRSLGFVESRKSGNLVYYSCKNKAVRDLVEYILE
                     SNI"
BASE COUNT          700 a          810 c          800 g          909 t
ORIGIN      
        1 ttagataaac aggttcatgt tggagttatc gctgtcgttg agcatggtgg caacgccacc
       61 gatttgcacg ccgtccagca actcttcctg ttttagcccc atgatgtcca tggtcatctg
      121 gcaagcgacc agctccacgc cggcgtcaat cgcctgttgg ataagctgtt caaggctgtc
      181 gatgccttta tgcttcatga tgccacgaat catttttgcg ccggcgccag ccatattcat
      241 ttgcgaaagc cccagcttgc gcgagccctt ggggagcatc atgccgaaca ttttgcccat
      301 gaaatctttt ttgacgggca catattgttc cttgcgcagg atactcagac cccagaaggt
      361 gaagaacatg tgcaccttgt tgcccatggc ggccgcgcca ttagcgataa tgaaagcggc
      421 gatggcttta tcgaggtcgt tatcgaaaat gatcatggtc ttttctttta gcggagcgct
      481 tgcctgcgcc acggtttgtg gcaaggtgcc agcgccagct gaaagtccct tttgcacgat
      541 agcccgatag atgccttttt cgctttcgct tttaatcagc gtgttgccgg tgtttttaca
      601 ccaggcggcg atatcgcggg tgaagcctgg atcggaggcg gtcacatcca gctgatcccc
      661 atcgttcagg gtcttgagtg tgtttgctac ctgcacgata ggaccagggc agctcaatcc
      721 gcaaacgtcg agggtttcca gtttgccaac cgtgcttgcc acaggcgcga tttcagcggg
      781 gatatttgac gccttagtat ctggggttgt atttgtgttc attgtgatgt cgtactccat
      841 gtcgtggtag ctttgcaagc caccggcaaa gtttttgacc ttaaaaccat gttgtttcaa
      901 gagacgctca gaaataaaac cacggcggcc tacccggcag agaatcgcat aggttttgct
      961 tttatccagt tcgctgatgc gttcgcgaag ctgggaatgt gggataagca gcgcgccggg
     1021 gatgctgccg gctatggttt catccggttc gcgaatatcg agcaaggtgt aggcatcagg
     1081 gttttgggcc cactcgtgat agaaaatggg gtcggtcaag ccttccagaa tgttcatgcc
     1141 catatatccg gcaaaattta acggatcctt ggccgaagag tagggcggcg cgtaagccag
     1201 ttccagttgg ctgagatctt ttaccgtgcc tttgaaataa atgacgcttg ccagcacgtc
     1261 gatgcgttta tccacgccct catagcctac aatttgcccg cccagcactt tgccttcttt
     1321 gtcgaaaata acttttaccg aaaggggcaa cgcgccgggg aagtaaccgg aatggcattt
     1381 ggggtgcgaa agcgcgatga agtaatcctg gccatatttc tgcccggctt tgttgagcgt
     1441 tttttcgttg gcgcccacac tggcggcggt tatatcaaag agcctcagaa tgctggttcc
     1501 catcgtgcct acataagtct cgggcttttt gccaagtatg ttcgcagcca ccgcgcggcc
     1561 ttgtttgttc gccggccctg ccaggggaat catggttggc gttcccatga cataatcctg
     1621 cacgctgata acatctccca ccgcgtaaat atcggggtcg gatgtttgca tatactcatc
     1681 caccagaatg ccgccccgtt cgctgagact caagccggct tctttggcca gttcgttgtt
     1741 ggggcgcacg ccgatggaaa gcacaaccat gtccgcttca agcgcctggc catcactgag
     1801 cattactttt tgcccatctt ccgacatgcc agaaaaggct ttgcccaact tgagcgtgat
     1861 gccattttcc gccatgtggt tttcgaccag tttggccatg tcagcatcca gtgggggcat
     1921 gacctgattc atcatctcga cgacggtcac tttcaagccg cggtgggcca gattttctgc
     1981 catctccagc ccgataaagc caccgcccac cacaaccgcg ttttggggct gcttttcctg
     2041 gatgaactgg acaattttat ccacgtctgg aaccgtccaa agttgaaata cgttggggtt
     2101 atccgtgccg ggcactttgg gcacgaaggg ccgcgaaccg gttgagagta aaagcttatc
     2161 gtatgtttcg gtgtagtgtt tctcgttggc gcggtcataa acatcgaccg tcttgtttgc
     2221 cgggtcaatg cgaatgactt cgctttcaac gcggatgtca acgccatagt ctttttcaaa
     2281 tttttccttc gtggagacga acaaggcatc gcgcgaagca atcgccccgc ccacgtaata
     2341 aggcaagcca caattggcaa aactgatgta ctttccgcgc tcaaagagaa cgatttcggc
     2401 gttctcatca agtcttctca agcgcgccat ggcggttgct ccgccggcga caccgcctac
     2461 gataacaatt ttcataattt agctcctttt agatattgct ttccaaaatg tattcaacca
     2521 ggtcgcgaac ggccttgttt ttgcaggaat agtaaacaag attaccactc tttcgggatt
     2581 ccacaaagcc caaactgcga agtctgccca ggtattgcga aatgagagat tgactcgcgc
     2641 ccatacatgt gaccagttcg gaaacggtcg tctcacctct tttgatgatt tgttccatca
     2701 ggcatagccg cacagggtta gccagcgctt tgagtatttc agccttggct tccagttctt
     2761 ttcggtgtgt ttcgttttct gagagtggca gatcgttttg tttcataatt cccttcctgc
     2821 attcaggtat tagtatatct taatatgttg atataggggt gatttgtata agctggaaat
     2881 aatgtatttt ggtgtattgt gaaaggaaat cagccttatg aagaagccaa ggataatgtt
     2941 ttggctttgg cttataaact caaaaagtga aaccaagctt ttttaagagt ggcattaatt
     3001 ttctttcagg ggtgttatta cctctgattc aacaaactgg aatttattcc ttcaccactt
     3061 cttcaattag ccttaccgct tccgtcacgc caccttcttt tttgatttta gcggaaagga
     3121 gatccacgtt ctttttcttt tcctcgtgcg tgctttgcat gtcaaatatg gcttggtata
     3181 tcgactcttc actcaaatct ttcgacttga gaggttttg
//
LOCUS       DAOSFC010000167         3174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_28684_length_3174_cov_4.987496, whole genome shotgun sequence.
ACCESSION   DAOSFC010000167 DAOSFC010000000
VERSION     DAOSFC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28684_length_3174_cov_4.987496"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PK791_04925"
     CDS             <1..1037
                     /locus_tag="PK791_04925"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOH92690.1"
                     /translation="QTVPNNRHFDTTMGNLQVIGANPVDLPVEESEDSDLIAPFKGNM
                     DLVKLEELIKRVGVENIPIGMLTITNNSAAGQPVSMDNIRGTSEIYKKYGIPFFIDAC
                     RFAENAWFIKLRDPKYKDVPVREIVREMFSYADGCTMSSKKDAIVNIGGFVAFRDKEL
                     KGKIGANLIVHEGYLTYGGLAGRDLDALAVGLYEGTDEEYLNYREAHTRYLGEVLSAA
                     GLPVYKPTGGHAVYVDAGKALPWIPPSQFPAVALANALYIEGGIRTVEIGSLMFEHTD
                     PETGEQVYSPLELLRFTIPRRVYTRSHLDFIGEVAKRVMENPKDIKGLRVTWAPEVLR
                     HFMAELEEVE"
     gene            1201..2574
                     /locus_tag="PK791_04930"
     CDS             1201..2574
                     /locus_tag="PK791_04930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92691.1"
                     /translation="MLTRLKTFVKNQFAIRPEDNVHPEVARLFKHNAIVNTFDICFFF
                     MADSFWAINTIMPVFAATLTDSPFIIGLMPAIVNAGWFLPQMFMAKRVSEMPEVVTFG
                     KRMARLERIPYVFFIILALLLPVIGNKIGLIALILVMIWRGVGGGMSALPWQETMARV
                     IPLSHRARFFGFSRVIGQLFGVVGSAIMALILARVDYPYNYALGFGVAVVIQWVSYAF
                     YHQNREPDINIPQVQDEQSNATMAIRQNTSADAHDAPIPGVMEILRKDRNFRLYIIAR
                     ALSFLGNMATAFIAVYAIQTFNLNDEQAAIFTGVLFAAGIFGYALWGAIGDRIGPKKI
                     VTYSFVAWAIALLIAIFTNTVWVYYLVFVFFGIYSAGIGVGDSMLIMELSDDRLRPTY
                     LGMARTLTGAFLLLAPVLAGWLVYEYSYNLMFWIALAFTLVSTVLMAQVRDFPRGKRV
                     LTKDGDV"
     gene            2653..3105
                     /locus_tag="PK791_04935"
     CDS             2653..3105
                     /locus_tag="PK791_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HOH92692.1"
                     /translation="MKCNNCQFENAPGATACAQCGSPLPVPQQGGYTQQGYAAPPQYG
                     YRRMPVNCQVCGAMNPPGTTVCEKCYAATDPNAVYSDKNRTTALVLALLLGIVGGHRF
                     YVGKTGMGILYIFTGGLFGIGVLVDVIKIITGDSRDSNGYPLRKGCFI"
BASE COUNT          785 a          705 c          776 g          908 t
ORIGIN      
        1 gccaaactgt gccaaacaac cgtcactttg ataccaccat gggcaatttg caggtcatcg
       61 gcgctaaccc cgtcgattta cctgtggaag aatcagaaga ctctgactta atcgcgcctt
      121 tcaaaggcaa tatggacctg gtaaaacttg aagaactcat caaacgtgtt ggcgtagaaa
      181 acattcctat cggtatgctg accatcacca acaactctgc tgcaggtcaa ccagtatcta
      241 tggataacat tcgtggcact tctgaaattt ataagaaata tggcattcca ttctttatcg
      301 atgcctgtcg ttttgctgaa aatgcctggt ttatcaaact tcgcgatccg aaatacaaag
      361 atgtacctgt acgtgaaatc gtgcgcgaga tgttctctta tgctgatggt tgtacgatga
      421 gctccaagaa ggacgctatt gttaacatcg gtggttttgt ggcattccgc gacaaagaac
      481 tgaagggcaa aattggcgcg aacctgattg tgcacgaagg ctatttgacc tatggcggat
      541 tggcaggacg cgacctggac gctttggctg ttggcttgta tgaaggtact gacgaagagt
      601 acctaaacta tcgcgaagcg cacacacgct acctgggtga agtcttgagc gcggcaggtt
      661 tgccggttta caaaccaacc ggtggtcatg cggtgtatgt ggatgcgggt aaggctttgc
      721 cgtggattcc accttcacag ttccctgcgg tcgcgctggc taacgcctta tacattgagg
      781 gcggtatccg cacggtcgaa atcggcagcc tgatgtttga acataccgac cccgaaacgg
      841 gtgagcaagt ttactcgccg cttgaactct tgcgctttac gattcctcgc cgtgtctaca
      901 cgcgtagcca tttagacttt attggcgaag ttgccaaacg cgtgatggaa aacccgaaag
      961 acatcaaagg cttgcgcgta acatgggcac cagaagtttt gcgtcacttc atggcggaac
     1021 ttgaggaagt ggaatagcaa ctaaaaacca ttacttttgg aaatagagtt ttaaattgta
     1081 ggatttatag ggcttgtgag aacgagccct ataatgatgt gccttgtata tgtctaagct
     1141 cctatacgtc actaaggtca tattttttga gcgtatagcg tataatctgg tttaataact
     1201 atgcttacac gattaaaaac attcgtaaaa aatcaatttg ccattcgacc tgaggataac
     1261 gtgcatcctg aggtcgcgcg cttgtttaag cataacgcca ttgttaatac ttttgatatt
     1321 tgctttttct ttatggcgga cagtttttgg gcgattaata ccatcatgcc tgtttttgcg
     1381 gcaacgctaa ctgactcgcc atttatcatt gggctgatgc ccgctatcgt caatgcgggt
     1441 tggttcttgc cacaaatgtt tatggccaag cgtgtttccg agatgcccga agtggtgact
     1501 tttggcaagc gaatggcgcg tttggaacgc attccatacg tcttctttat cattttggca
     1561 ttattgctgc ctgttatcgg caataagatt ggtcttattg cgttgatatt ggtgatgatt
     1621 tggcggggcg ttggtggtgg gatgtcagct ttaccctggc aagaaacgat ggcgcgggtc
     1681 attccgcttt cacaccgcgc gcggttcttt ggcttttcgc gcgtcatagg acagctattc
     1741 ggcgttgtgg ggtcggcaat catggcactc attttagcgc gcgtggacta tccatataat
     1801 tacgccctgg gttttggcgt tgcggtcgtc attcaatggg tttcttatgc cttctaccat
     1861 caaaaccgtg aacccgatat aaacataccg caggtccaag atgagcaaag caatgccaca
     1921 atggcaataa ggcagaatac gtctgctgac gcacatgacg cacctatacc tggcgttatg
     1981 gaaatcctac gcaaggacag gaatttcagg ctttatatca tcgcgcgggc tttatcattt
     2041 ttaggcaata tggcaacagc ttttattgcg gtttatgcca ttcaaacttt caacttaaat
     2101 gacgaacaag ctgctatttt tacaggcgtg ctatttgcag caggaatctt tggctacgcc
     2161 ttatggggtg caattggcga tcgtatcggc cctaaaaaaa tcgtgaccta ttcttttgtg
     2221 gcatgggcaa tcgccttgct tattgccatt tttacgaata cagtctgggt ttattatctc
     2281 gtttttgttt tctttggaat ctactccgca ggcattggtg taggggactc aatgttaatt
     2341 atggagctta gtgatgacag actgcgtcca acgtacttag gtatggcacg cacgctgaca
     2401 ggtgcctttt tattgcttgc cccagttttg gcaggttggc tggtttatga atacagctat
     2461 aatcttatgt tttggatagc actggcattc acgctggtca gcaccgtgct gatggctcaa
     2521 gtgcgagact ttccgcgggg taagcgcgta ttaaccaagg atggtgatgt gtaaaatatt
     2581 tacacaagtt tcataactac cttacagaat tcgagtaaaa taaaaataca taatattaaa
     2641 aaggagttcg aaatgaaatg taataattgt caattcgaga atgcacctgg cgcgacagct
     2701 tgcgcgcaat gtggaagccc attgccagtc ccccaacaag gtggctatac ccagcagggc
     2761 tacgccgcac caccacagta cggatatcgc cgcatgccag taaactgcca ggtttgtggc
     2821 gcaatgaacc cacccggaac caccgtttgc gaaaaatgct acgctgctac tgatcccaac
     2881 gcggtttaca gtgacaaaaa ccgcacgacc gcactggttt tggctctctt gcttggaatc
     2941 gttggcggtc accgctttta cgtaggcaag acgggtatgg gtattttgta tatctttacc
     3001 ggtggcttgt ttggtatcgg tgttttggtg gatgtcatca aaatcatcac cggtgattcc
     3061 agagacagca acggttatcc cctgcgtaaa ggttgcttta tctaaaccat taccctttct
     3121 ttgattaagt ttgccacgtc gctgtaattg aggcgacgtg gtgcatttaa ggca
//
LOCUS       DAOSFC010000168         3170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_28736_length_3170_cov_3.711075, whole genome shotgun sequence.
ACCESSION   DAOSFC010000168 DAOSFC010000000
VERSION     DAOSFC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28736_length_3170_cov_3.711075"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PK791_04940"
     CDS             <1..430
                     /locus_tag="PK791_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92693.1"
                     /translation="YIAFYQTAAFNERERWQIRWMAPIQGHELTTRLDLFRDEADHPR
                     ASEEYFKLQLGPLIELPRPIQAGDWKRVTFFYTTGERCKRAEKLNDLPVHDEERQILW
                     RSLRERALRGHEYNAQELPEFAIDPAILAFLSGFQGTNLS"
     gene            443..2056
                     /locus_tag="PK791_04945"
     CDS             443..2056
                     /locus_tag="PK791_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92694.1"
                     /translation="MAFFIVLGLLVIASFLIVVLQRGKSKVGLAWFIALVASAAAWFV
                     LFLLRLFLPLNLELLSWVPTELFQGTLALSLDYFSWPYAIAIMTLCVASIFTETTVAS
                     KKDSSPRWVASLTLTALTLVSLASTTPLTLTITWSLIDIAELFYRLRAANPEKDQGAI
                     VRIFSLRVFGTFALIFATAVAWQVQPDFSFDQIPSTAGLFFLLAAGLRLGVLPVHLPF
                     TQGGADSQESKFLFDLAPVASSLTLIARLPGDFLTLNRTLLIIVQVLSAIAMLYGAMM
                     WISRKTATQARPYWIITLSAFALQCALNGDPGSSRVWGLALLLSGGLLFSFNPPIRRI
                     RFLPALGLVGLLGLPYTLCASGWQGVLGQNFGIVSVGVLISHSLLVLGYVRYIAEASG
                     VVTGLEKHARVTFPLGLIIIFQTIVLLGLIAWPGVLTAGVWWASLISLAITVLVIIAL
                     RVLGIRDLQQDLANKLPFYRILSPIIRFLRTFFSLSWLYNALTWIYSQVFRLENFAST
                     IIEGDGGLLWSLVFLLIILLFLASGVRLS"
     gene            2053..2652
                     /locus_tag="PK791_04950"
     CDS             2053..2652
                     /locus_tag="PK791_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92695.1"
                     /translation="MTKFLLTAAVITGLLAALANLVRRKWLFNILALAIQYVCIFIVF
                     PKTDGFMHALIKIIVGLMSTLIIYLTIQSTGAVKREPFEFKLSAGELFRMLSAVFLVT
                     LVVLASPWLLKEVFPQSNIYVLILSLGLALIGLLQLGSKTESLYVIIGLLTFLSGFEL
                     LYGSLEFSALLEALFAIVNLLIAIAGAFFIVKDLETETP"
     gene            2649..>3170
                     /locus_tag="PK791_04955"
     CDS             2649..>3170
                     /locus_tag="PK791_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92696.1"
                     /translation="MINNTVIIWILIPVALSGILALLQRFAKLSTAIVLVSSALLTVL
                     AFILPEDLILSIGSRQIAFADSLEIFGRVLKITKANLSLVGGIYLCVFLWNSLARKLE
                     TSQWFNALSLLIAALWISALAVQPFLYASVIILLVAVVSIPLLSPRGSRSGAGVLRYV
                     TLQAIAMPLILLSA"
BASE COUNT          645 a          848 c          715 g          962 t
ORIGIN      
        1 ttacatcgcc ttttatcaaa ccgccgcttt caacgaacgc gaacgctggc aaattcgctg
       61 gatggcgcct atccagggac acgaactcac cacacgactg gatttattcc gtgatgaagc
      121 cgaccacccc cgcgccagcg aagaatactt caaacttcaa cttggcccac tcattgaact
      181 gccccgaccc attcaagctg gcgactggaa gcgcgtgaca ttcttctata caacagggga
      241 acgctgtaaa cgcgccgaaa aactgaatga tttacccgtc cacgacgaag aacgtcaaat
      301 cttatggcgt tctctgcgcg aacgtgccct gcgcggacac gaatataacg cccaggaact
      361 gccagaattc gccattgacc ctgccattct ggccttttta agtggttttc aaggtacaaa
      421 tttaagttag aattaagcca acatggcatt tttcatcgtt ctgggattac tggtcatcgc
      481 gtcctttctt atcgtcgtac tccaacgcgg caaaagtaag gtcggtttag cttggtttat
      541 agccttggtc gcatcggctg cggcatggtt tgtacttttt ctgttaaggc tctttttacc
      601 actcaacctg gaacttttaa gctgggttcc caccgaactg ttccaaggca cgctcgcgct
      661 tagcctggat tatttcagct ggccctacgc cattgccatt atgacccttt gcgtagccag
      721 tatttttacc gaaacaacag ttgcttcaaa aaaagactcc agcccacgct gggtggcatc
      781 actcacgctc accgcgctca ccttggtatc gcttgccagt accacaccgc tgactttaac
      841 tatcacctgg tctttaattg atatcgctga acttttttac cgcttgcgcg ccgcgaaccc
      901 tgaaaaagac cagggcgcca tcgttcgcat tttctcgctg cgtgtgttcg gcacatttgc
      961 gcttatcttt gcgacagcgg tcgcctggca agtccaaccc gactttagct ttgaccaaat
     1021 tccttccacc gctggattgt tctttctgct tgcagcgggt ttgcgtttgg gggttttgcc
     1081 cgtgcacctg cctttcacac aaggaggtgc tgacagccag gagagtaagt tcctgtttga
     1141 cctggcaccg gtggcatctt ccctaacgct catcgcgcgt ttacctggtg attttctcac
     1201 cctgaaccga actttgctca ttatcgtgca agtcttaagt gccatcgcga tgctctatgg
     1261 cgccatgatg tggattagcc gcaaaactgc cactcaagcc cgcccctact ggattatcac
     1321 gctttccgcg tttgccttac aatgcgctct gaacggcgac cccggcagca gtcgcgtgtg
     1381 gggcttggca ttattgctga gtggtggcct actgttcagc ttcaacccac ccattcgccg
     1441 tatccgcttt ttgcccgctt tgggtctggt gggcttgctt ggcttgcctt ataccctgtg
     1501 cgcgtcaggc tggcaaggcg ttttggggca aaactttggc atcgtcagcg ttggcgtgct
     1561 cattagccac agtctgctcg ttctgggcta tgttcgctat atcgctgagg ccagtggcgt
     1621 tgtaacgggt ttggaaaaac atgcccgcgt cacttttccc ctgggtctca tcatcatctt
     1681 tcaaaccatc gtcttgctgg gtcttatcgc ctggcctggc gtcttaacag cgggcgtttg
     1741 gtgggcgagt ctcatcagct tagcgataac cgttttagtg attatcgcct tgcgcgtctt
     1801 gggcattcgt gacctgcaac aagatttagc caacaaactt cctttttacc gcattctatc
     1861 gcccattatt cgctttctgc gtacgttttt cagccttagc tggctataca atgccctcac
     1921 ctggatttat agccaggtgt ttcgtcttga aaactttgcc agcacgatta ttgaaggtga
     1981 cggtgggttg ctttggtctc ttgttttcct cttaatcatc ttgctcttcc ttgcctcagg
     2041 agtaagactg tcatgaccaa gtttttgctg actgctgcgg tgataacagg acttttggct
     2101 gccctggcga atttagtgcg ccgtaaatgg ctctttaaca ttttggcact agccatacaa
     2161 tatgtttgta tatttatcgt tttcccaaaa acagatggct ttatgcacgc cttaataaaa
     2221 attatcgttg gcttaatgtc aacgctgata atttatctga ctattcaaag cacaggtgcc
     2281 gtaaaacgcg aaccatttga atttaagctc tcagcaggtg aactattcag aatgctcagt
     2341 gctgttttcc tggtaacgct ggttgtttta gccagtccct ggcttctgaa agaagtcttt
     2401 ccacaaagta atatttatgt cttgatattg agtttaggct tagcgctcat cggccttttg
     2461 caactcggat ccaaaactga gtcgctttat gtcattatcg gtttattgac cttcctttct
     2521 ggatttgaac tgctttacgg ctctcttgaa ttttccgctt tgctcgaagc actttttgcg
     2581 attgttaatt tgctgattgc cattgctggc gcgttcttta tcgtcaaaga cctggagaca
     2641 gaaacaccat gataaacaac accgttatca tttggattct tattccagtc gctttgagtg
     2701 gcattttagc gcttttgcaa cgcttcgcca aactctccac ggcaattgtg ttggtcagtt
     2761 ccgcgctctt aaccgttctg gcgtttatct tgcctgaaga ccttatctta tccataggct
     2821 cacgccaaat cgcttttgct gacagtcttg aaattttcgg tcgggtgctt aaaatcacca
     2881 aagccaacct tagcctggtt gggggcatct atctctgtgt tttcctgtgg aacagcctgg
     2941 cacgcaaact tgaaacaagc cagtggttca atgccttatc gctattgatt gcggctttgt
     3001 ggataagcgc tcttgccgta caacccttcc tttatgcttc tgtgattatt ctcctggtcg
     3061 ctgtcgtcag cattcccttg ctttctccgc gcggatccag gtctggcgcg ggcgtgctgc
     3121 gctatgtcac cttgcaagcc attgccatgc ccctgatttt gctcagcgcg
//
LOCUS       DAOSFC010000169         3165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_28794_length_3165_cov_3.590997, whole genome shotgun sequence.
ACCESSION   DAOSFC010000169 DAOSFC010000000
VERSION     DAOSFC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28794_length_3165_cov_3.590997"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /gene="cas7c"
                     /locus_tag="PK791_04960"
     CDS             complement(<1..669)
                     /gene="cas7c"
                     /locus_tag="PK791_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOH92697.1"
                     /translation="MENKTPLSNRYEFVLFYDVENGNPNGDPDAGNMPRIDPETGYGI
                     VTDVCLKRKIRNYVDIIKSDQPGFQIYVTEGIALNSQHLKAYTETNTPLPKQAKNVPA
                     DDLQKVRKWMCDNFYDVRTFGAVMTTGVNCGQVRGPVQLNFSRSLDPIMQQEVTITRV
                     TVTRPEDEKTTEMGRKHIVPYALYRVEGYISPALANHENKGTGFSEEDLELLWLALEN
                     MFEFD"
     gene            complement(688..2583)
                     /gene="cas8c"
                     /locus_tag="PK791_04965"
     CDS             complement(688..2583)
                     /gene="cas8c"
                     /locus_tag="PK791_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOH92698.1"
                     /translation="MILQNLAKLYDQLLDDESVKIPQPGFSVVNINYFLSISASGELL
                     DIIYVFDETTSGKKTVERPQKIILPEAVKRSSGIAPNLLWDNSAYVFGLADESKSFQY
                     CKDRFEAFREHNIAFLSQLNSPETNALLNFLSQYSQEDLITHPQILAKRDEILKATGF
                     LTFQYEPTKKLIANLKTVRKAVSSQWEDSGEDAVKAQCLITGQIAPVQRLHAAIKGVK
                     DVQSAGGSIVSFNDRAYESYGKEQKQGLNAPVSTSAAFAYTTSLNYLLSPENPHKKLQ
                     LGDSTVVYWAESTNPTYENLFQAIIDPQWDIETSPEDEQTQNTRYDHETTSILQKIAG
                     SIRDGLPIDFNALDENLDPSTKFHVLGLAPNAARISIRFYETAPFSKILSRLIQHHED
                     ISMGQDRPWSVWRLLKETISPKASKQEASPLMAGAIMRSILTGLPYPMALYYAILNRV
                     RADSDDPTKGVYKISAIKAGIIKACLLRKARQQNNTHLQEILTMSLNPQSTNQAYLLG
                     RLFAVLEKAQEDAASPNKLNATIKDRYFTAASANPASTFPVLLRLSQHHISKSEWGRI
                     SEIRIGEIMELLDIDDTPFPKRLSLDEQGIFILGYYHQRNDFYKSKKQEPSAELESSD
                     ITQTELF"
     gene            complement(2586..>3165)
                     /gene="cas5c"
                     /locus_tag="PK791_04970"
     CDS             complement(2586..>3165)
                     /gene="cas5c"
                     /locus_tag="PK791_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOH92699.1"
                     /translation="PSPSAVRGIIEAVYWKPAIRWVIDRIHVLNPILFTNIRRNEVSD
                     KVPFNTAKSLMQGNTNKDFYLDILTARQQRASMILKDVDYVFEVHFEMVPEEAGPTDT
                     VEKHYNIALRRLRKGQHYTQPVLGTREFPAKVSLIENRDEIPKSVLEGEQDLGWMLYD
                     MDYSDPENILPIFFKAKMIDGIIDLQNVLKAS"
BASE COUNT          759 a          593 c          816 g          997 t
ORIGIN      
        1 atcgaattca aacatgtttt cgagcgccag ccacaggagt tcgagatctt cttcggagaa
       61 gccagtgcct ttgttttcat gattagccaa agcgggagag atgtaacctt caacgcggta
      121 gagagcgtaa ggcacgatgt gtttgcggcc catctcggta gttttctcgt cttctgggcg
      181 ggttacggta acacgtgtga tggttacttc ttgttgcatg atagggtcta gactgcgcga
      241 aaagttgagt tgcacaggac cacgaacctg accgcaatta acgcctgttg tcatgacggc
      301 gccaaaagtg cggacatcgt aaaagttgtc gcacatccat ttgcgtactt tttgcaaatc
      361 gtctgctgga acgtttttgg cttgtttggg caagggcgta ttggtctcag tataagcttt
      421 gagatgctgg ctattgagcg caataccttc ggttacgtag atttgaaagc cgggctggtc
      481 tgatttgatg atgtcgacat agttacggat ttttcgtttg agacaaacat cggtgacgat
      541 gccataaccg gtttccgggt caatgcgggg catattgccg gcatctggat caccattggg
      601 gttgccattt tcaacatcat aaaaaaggac aaattcgtaa cggttgctta gtggggtttt
      661 gttttccatt ttttaactcc tgtttgttta gaatagttct gtttgggtaa tgtcggagct
      721 ttcaagctct gctgatggtt cttgtttttt ggatttatag aagtcatttc gttgatggta
      781 ataaccaagg atgaatatgc cttgttcgtc aagactgagg cgttttggga agggcgtatc
      841 atcaatatct aaaagttcca tgatttcgcc gattcttatt tcggaaattc ttccccattc
      901 cgattttgaa atgtggtgtt gcgacaagcg taaaagaaca ggaaaggtgc tggcaggatt
      961 ggcactggct gctgtgaaat agcggtcttt gatagtcgca ttcagtttgt taggtgaagc
     1021 ggcatcttct tgcgcttttt caaggacggc aaataaacgg cctaagagat aggcttgatt
     1081 ggtggactgt ggattaagcg acatagttaa aatctcctgt aaatgtgtgt tgttttgttg
     1141 gcgcgctttg cgcaggagac atgctttaat gatgcctgct ttgatagcgc tgattttgta
     1201 aactcctttg gtcgggtcat cagaatcggc acgtacccga tttaaaattg cataatagag
     1261 agccatgggg tagggcaaac cggttagtat agagcgcatg atggcaccgg ccataagagg
     1321 agacgcctct tgtttgctgg cttttggcga aatggtttct ttaagtagtc tccataccga
     1381 ccaagggcga tcttgaccca tggaaatatc ttcgtgatgc tgtatcagac gggataagat
     1441 ttttgagaat ggggcggttt catagaatct tatggaaatt ctggcggcgt tgggtgccaa
     1501 acccaagacg tgaaatttgg ttgatggatc taagttttca tcaagggcgt tgaagtcaat
     1561 tggcagacca tcgcggatac ttcctgcaat tttttgaagg atggaagttg tttcgtgatc
     1621 ataccttgtg ttttgcgttt gctcatcttc agggcttgtt tcaatatccc attgtggatc
     1681 gatgatggcc tggaacaggt tttcgtaggt ggggtttgtg ctttctgccc agtagaccac
     1741 tgtagaatcg ccaagctgaa gttttttgtg tggattttca ggcgataaga gatagttgag
     1801 actggtggta taggcaaaag cagcggatgt actgactggc gcgttcaaac cctgtttttg
     1861 ttcttttcca tatgattcat aagcacggtc attgaatgaa acgatagagc ctccagctga
     1921 ctgtacatct tttacacctt ttatcgctgc atgtaggcgc tgaactggag caatttgccc
     1981 tgtgattaaa cattgtgctt tgacagcatc ttcgcccgaa tcttcccact gggaacttac
     2041 agctttgcga actgttttta aattggcaat tagttttttg gtgggttcat attggaatgt
     2101 cagaaaaccc gtcgctttca gaatttcatc acgttttgcc aggatttgag ggtgtgtgat
     2161 gaggtcttcc tggctatatt ggcttaggaa gttaagtaaa gcatttgttt caggggaatt
     2221 aagttgactt aagaatgcaa tattgtgttc tcgaaatgct tcaaacctgt ctttgcaata
     2281 ctgaaaagat ttactttcat cagcgaggcc gaaaacataa gcagagttat cccataatag
     2341 gtttggtgca attccagagg atcgtttcac tgcttctggc aagattattt tttgtggtct
     2401 ttcaactgtc ttcttgccgc tcgtggtttc atcaaacaca tagataatgt ctaaaagttc
     2461 accgcttgcg gatatgctca gaaagtaatt gatgttcaca acgctaaatc cgggttgggg
     2521 aatttttacg ctttcatcat caagcagttg gtcatagagt ttggcaagat tttgaagaat
     2581 catcgctaac tcgctttcag tacattttgt aagtcaatga tgccgtcaat cattttggct
     2641 ttgaagaaga tgggtaggat gttttcgggg tcggagtagt ccatatcgta gagcatccaa
     2701 ccgagatctt gctcaccttc aagaacgctt ttggggattt catcacggtt ttcaatgaga
     2761 ctgactttgg cgggaaattc acgcgtgcca agcacgggtt gggtgtaatg ttgtcctttg
     2821 cgcaggcggc gcaaggcaat gttgtagtgt ttttcgacag tatcagtggg tccggcttct
     2881 tcaggcacca tttcaaagtg gacttcaaaa acatagtcaa catcttttaa aatcatcgag
     2941 gctctttgct gccgtgcggt gagaatgtcg agataaaagt ctttgttggt gtttccttgc
     3001 atcagagatt tggcggtgtt aaaggggact ttgtcggaaa cttcgttacg tcgaatattg
     3061 gtgaaaagaa tagggtttaa tacgtggata cgatctataa cccagcggat ggctggtttc
     3121 cagtagaccg cctcaatgat tccccggact gctgatgggc ttggt
//
LOCUS       DAOSFC010000170         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_29303_length_3127_cov_5.988607, whole genome shotgun sequence.
ACCESSION   DAOSFC010000170 DAOSFC010000000
VERSION     DAOSFC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29303_length_3127_cov_5.988607"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PK791_04975"
     CDS             complement(<1..1128)
                     /locus_tag="PK791_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOH92700.1"
                     /translation="MNKNIKHLLHIALVAIMLFGVSACQSNGEETLTASGTLSALQVS
                     VVPEVSGKIVEITVEEGSQVKAGDVLIRLEDDVLVAQLNQADAAVAVAEASLEAAHAQ
                     LDYVKAQRDLAIDGARYNDLQARQNAWAQPAPEDYQPVWYFSKAEQQIAAQAAFEEAE
                     TKLEERQEMLENELDKAGAKNFVDIEAELAKAQAQLNIANLTLTIAQQSPDERLKDAA
                     EKAQELAQSAFDNVLKRYNDALSTSAAEDVLDARAELAVAQSSYDSALDLLMSLRTGE
                     DSGQVRVAQNGVIQAQAAIGQAEAGLEQAKAAQALAQLQLDRAEIKAPIDGVVITRNV
                     EVGDIAMAGGNVMSIADMSEMDLVVYVSETWYGQLKLNQEVK"
     gene            complement(1155..2267)
                     /locus_tag="PK791_04980"
     CDS             complement(1155..2267)
                     /locus_tag="PK791_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH92701.1"
                     /translation="MRKEVRHILRDPLTLLLILALPASLLVLLGYGITGESSGVTLAI
                     VDFDKSDSSRAYVQRFTSSDDFRLIQDLSNVKELERQIEIDKVDVGIIIPESFERELL
                     NNGTSNVMILLDGSSNPTDSTTSQLKLTAISEMANQDILMKQVDKSGQAHQLRMPINT
                     TVQTLYNPNGNVRLFMIPGLITIILQVQTILLAALSIVREREQGTMEQLIVTPIKSWE
                     LMLGKIIPYLIVCLLNLFLLLWMAEVMFGVHVMGNMGELIGLSVIFILGSLGMGVLIS
                     NISKTQMQAVYISVFLVIIPAVILSGLMFSRESMPVFTYWYSELLPVTQYLEITRGII
                     VRGVDAITLLYTSTIPLIVLSVGYFIASVFAFRKHL"
     gene            complement(2313..>3127)
                     /locus_tag="PK791_04985"
     CDS             complement(2313..>3127)
                     /locus_tag="PK791_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92702.1"
                     /translation="GKTTTIRMLIGILRPDSGTANVLGFDVRKKPEEVKKRIGYMSQK
                     FSLYNDLSVRENLDFYAAIYNDISTRQRRIRVDEMLERVDLTDMQKSLTRSLSGAWRQ
                     RLALACAIVHKPKMLFLDEATAGVDPVARRYFWDLIYRLAGEGISVLATTHYMDEADY
                     CNTIGMMYQGRLVSLASPDTIKDSLPGVLLQVDVEDPLEVQKVLQNVPCVLHISIQGM
                     QLHVTVKRESDIGTIQRTLARANMPPSNIEIIQPSLEDAFTSIVSQERDKAS"
BASE COUNT          770 a          806 c          762 g          789 t
ORIGIN      
        1 tttgacttcc tggttgagct tcagctgccc ataccatgtt tcagacacat aaacgaccaa
       61 atccatctcg ctcatatcag cgatgctcat cacattgcct cccgccatgg caatatcacc
      121 cacttcaaca ttacgggtta tcacaacacc atcaataggt gctttaatct cagcacggtc
      181 gagttggagc tgcgccagcg cctgggcagc tttggcttgt tccaaacctg cttcagcctg
      241 gccgatagca gcctgcgctt gaatgacccc attttgagca acccgaacct ggccagagtc
      301 ttcgccagta cgcaggctca tgagcaaatc cagggcactg tcatagctgc tttgtgccac
      361 ggcaagctcc gcccgcgcgt ccagcacatc ttccgcggcg gatgtggaga gcgcgtcgtt
      421 gtagcgcttg agaacgttat caaatgcgga ctgtgccagt tcctgggcct tttcagcggc
      481 atccttcagg cgttcgtcag gactttgttg cgcgatggta agcgtgagat tagcgatgtt
      541 gagctgggct tgcgccttgg ctagttcagc ttcaatatcc acaaagttct ttgccccagc
      601 tttgtcaagt tcattttcca gcatttcctg gcgttcttcc agttttgtct cagcttcttc
      661 aaacgcggct tgagcggcga tttgttgttc agctttgcta aagtaccaca ctggttgata
      721 atcctccgga gcaggttgcg cccaggcatt ttggcgagcc tgcaagtcgt tatagcgcgc
      781 gccatcgatg gcaagatcac gctgcgcttt gacataatcc agctgagcat gagcggcttc
      841 aagcgaagct tcggcaaccg ccaccgcggc atccgcctga ttcagctgag cgaccagcac
      901 gtcatcttca agcctgatga gaacgtcgcc agcttttacc tgactgccct cttcaaccgt
      961 tatttcgaca attttgccac tgacttccgg caccacgctg acctgcaaag ccgaaagcgt
     1021 tcctgaagca gttagcgttt cttcgccgtt ggattgacac gcactcacac caaagagcat
     1081 tattgccaca agagcgatgt gtaacaggtg tttgatattt ttattcatag gattcacctc
     1141 aatttcaata agtactaaag atgcttgcgg aatgcaaaga ccgaagcgat aaaatagcca
     1201 acgctgagta caatcagcgg aatggtagat gtatagagca aggttatggc atcaacacca
     1261 cggacgatga tgcctcgtgt gatttcgaga tactgtgtaa ctggcagaag ttcactgtac
     1321 cagtaggtaa agaccggcat gctctcgcgc gagaacatca agcccgagag aataaccgcc
     1381 gggataatga ccaaaaagac cgagatgtaa actgcctgca tttgggtttt ggaaatgttg
     1441 gaaatgagca cacccatacc caaggaaccc aaaatgaaga tgacactcaa gccgatcaac
     1501 tcgcccatgt tacccatcac gtgcacgccg aacatcacct cagccatcca aagcaacagg
     1561 aacaagttga gcaaacacac gatgagataa gggataatct tgcccagcat cagttcccag
     1621 cttttaatcg gcgtgacgat gagttgttcc atagtgccct gttcgcgttc gcgcacgatg
     1681 gaaagtgccg ccaaaagaat ggtctgcact tgcaaaatga tggtaatcag accgggaatc
     1741 ataaacaggc gcacattgcc attagggtta tagagcgtct gcacggtcgt gttgataggc
     1801 atcctcaact gatgcgcttg accgctcttg tcaacttgtt tcatgagaat atcctggttg
     1861 gccatctcgc taatcgccgt aagtttgagt tgcgatgtgg ttgagtcggt tgggtttgaa
     1921 gagccatcca gcaatatcat gacgttactt gtgccattgt tgagtagttc acgttcgaaa
     1981 gattctggga tgatgatacc cacgtcaact ttgtcaattt caatctggcg ttccagttct
     2041 ttgacattgc tcaggtcctg aataaggcga aaatcatcac tactggtaaa acgctgcacg
     2101 taagcgcggc tactgtcgct tttgtcaaaa tcgacgattg ccagggtaac cccgctggac
     2161 tcgccggtaa taccatagcc aagcaaaacc aggagcgaag caggcaacgc caaaatgagc
     2221 aacaaggtaa gcgggtcacg caggatgtgc cgcacctctt ttcgcataat tgtccaaagt
     2281 cttttcataa aatcctgtct ttctaagcct ttttatgacg ctttatcgcg ttcctgactg
     2341 acgatgctgg taaaagcatc ttcgagtgaa ggctggataa tttctatatt tgatggtggc
     2401 atatttgccc gcgcaagggt gcgttgaatg gtaccgatgt cgctttctct ctttaccgtc
     2461 acatgcagct gcatgccctg gatagaaata tgaagtacgc aaggcacatt ttgaagcact
     2521 ttttgaactt caagcgggtc ttcaacgtct acctgcagca aaacaccggg tagcgagtcc
     2581 ttgatggtgt ccgggcttgc cagggaaacc aggcgtccct gatacatcat gccgatcgtg
     2641 ttgcaatagt cggcttcgtc catatagtga gttgtggcaa gtacgctaat gccctccccc
     2701 gccaggcggt agataagatc ccaaaaataa cgacgtgcca cagggtcaac cccagcggta
     2761 gcttcatcga gaaagagcat ttttggctta tgcacgatag cgcacgctaa agcgagccgc
     2821 tgtcgccacg cgccagaaag cgagcgggtc aggctttttt gcatgtcggt aaggtctacg
     2881 cgttccagca tttcgtcaac acgtattctg cgctgtcggg tagaaatatc gttgtaaatg
     2941 gcggcgtaaa agtcgagatt ttcgcgcacg cttaggtcat tgtaaagtga aaacttttgc
     3001 gacatatagc cgatacgttt tttcacttct tccggttttt tgcgcacgtc aaaaccaagc
     3061 acgttcgctg tgccactgtc cgggcgcaaa atgccaataa gcatgcgaat tgtggtcgtt
     3121 ttgcccg
//
LOCUS       DAOSFC010000171         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_29601_length_3105_cov_3.976066, whole genome shotgun sequence.
ACCESSION   DAOSFC010000171 DAOSFC010000000
VERSION     DAOSFC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29601_length_3105_cov_3.976066"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PK791_04990"
     CDS             <1..306
                     /locus_tag="PK791_04990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH92703.1"
                     /translation="LVFSSLNSTESFGIVQIEALSQGTPIVASDLPGVRQPVLRTGMG
                     RIVPIRDSNALASAIIDVLSARADGARREIPHEYLAGFSQEQIALRYEKEFERLIDD"
     gene            299..1123
                     /locus_tag="PK791_04995"
     CDS             299..1123
                     /locus_tag="PK791_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92704.1"
                     /translation="MTNKLSSEDLDKIVENQCFHLPYFRAMLRAVEQSFYPREGLPEP
                     VLDVGSGDGHFAWAMLTQQAWFGFDPWLEPSKEARKYGLYHSLIISDAHRLPSVSGHF
                     ASALSNSVLEHIPDVQGVLDEVYRALQDGGVFYFAVPNHRWREKLWGQAIFKCLGLKR
                     LASAYERFFNKISRHVNLDAPEVWTERLKQAGFESVETFNYFPVWALRMLERGHAAGL
                     PNLLWKKLFGKWVLFPSRKNPFLRFNKIRKLVDDSKCEDGVCSFFIAKKGAGASEL"
     gene            1120..1929
                     /locus_tag="PK791_05000"
     CDS             1120..1929
                     /locus_tag="PK791_05000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOH92705.1"
                     /translation="MKRFLRKVAYYLWSVVLLLVYVKNWSTLFSIFLGKAYTGLRWLK
                     LRRANLTMAVTTKMEAWSVKESLLDRFYTRYSDPIQANWTIVDIGASIGEFCVEAAMQ
                     APDGRVIAFEPNPGSINILRQNVRVNSLKNVESYNVGIWKEKGEITLEINHDESIQAR
                     TLDCDKDAGPDSRRTSIPVLSLNEMMKLIDADKIDLLKIDTEGTEFEILMSQPAEVLA
                     RIERMILEVHERRPEKTVAQLGQFLESMGYKTRCHENYVHDHLYYLYAWRE"
     gene            1997..2311
                     /locus_tag="PK791_05005"
     CDS             1997..2311
                     /locus_tag="PK791_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92706.1"
                     /translation="MQEKKDQQNLREVSESREFDSRVSEYLKEKLCFWRKDFGVVSTD
                     GEERLDEQVSSGPAETPDRQGQWRQIFFAGFVFVLIAQIIFEFKLEIRHGWVFFLFFL
                     AW"
     gene            2470..>3105
                     /locus_tag="PK791_05010"
     CDS             2470..>3105
                     /locus_tag="PK791_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92707.1"
                     /translation="MQVLVWLVSIALLGYASFSGGEGKQADAEGEPVLSHQVKWKSWR
                     ELFADKHYLTVCVLVIVLVLLFQILFIRKTPVELVSQQVETFLAVEEIAGGSRALLFP
                     RDVVSEPLGYYWLALISRLFPASMRICAFHVANILSFCLGLAFLFRLAKLLFDKWVAI
                     VAVFLFGVGFWPILQNTALLGSSLVFPLLSTALFYLFRGLLREKRGDIFLFG"
BASE COUNT          696 a          576 c          902 g          931 t
ORIGIN      
        1 ctggtgtttt cgagtttgaa ttcgacggag agttttggca tcgtgcagat tgaggcactt
       61 tcgcagggta cgccgattgt ggcgagcgat ttgcctggcg tgcgacagcc ggttttgcgc
      121 acgggaatgg gcagaattgt gcctattcgt gacagtaacg cgctggcatc tgctattatt
      181 gatgtcctga gcgcgcgtgc tgatggcgcg cgacgtgaaa tcccgcatga gtatttggca
      241 ggttttagcc aggaacaaat cgcgttgcgc tatgaaaagg aatttgaaag gcttatcgat
      301 gactaataag cttagttcgg aagatttaga caagattgtt gaaaaccagt gttttcattt
      361 gccatatttt cgggcgatgt tgcgcgcggt ggagcagagt ttttatccgc gtgaagggct
      421 gcccgaaccg gttttggacg tgggttccgg tgatgggcat ttcgcctggg cgatgctgac
      481 gcagcaagcc tggttcggtt tcgacccctg gcttgaaccc agtaaggaag cgcggaaata
      541 tgggctttat cattcattaa taatctctga tgcgcatagg ttaccatcgg tttccggcca
      601 tttcgcgtcg gcgttgagta attctgtgtt ggagcatatc ccggatgtac agggtgtgtt
      661 ggacgaagtg tatcgcgctt tgcaggatgg cggggtgttt tatttcgcgg tgccgaatca
      721 tcgctggcgg gaaaagttgt ggggtcaggc gatttttaag tgcctgggtt tgaaaagact
      781 ggccagcgcc tatgaacgct ttttcaataa aatttcacgg cacgttaatt tggacgcgcc
      841 ggaagtctgg actgaacgcc taaagcaagc tggttttgaa agcgtggaga ctttcaacta
      901 ttttccggtt tgggcattgc gtatgcttga gcggggacac gcggcaggtt tgccaaattt
      961 actgtggaaa aagttgtttg gcaagtgggt gctgttcccg tcgcgtaaaa atcccttttt
     1021 gcgttttaat aaaattagga agttggtgga cgattccaag tgtgaagatg gtgtgtgcag
     1081 cttctttatt gccaaaaaag gcgcaggagc atctgagtta tgaagcgttt tttgcgcaag
     1141 gtcgcttatt atctctggtc ggtagtgttg ctgctggtat acgtcaagaa ctggagcacg
     1201 cttttttcga ttttcctggg taaagcttat acaggtttgc gctggcttaa gttgcgacgg
     1261 gcaaatctga ccatggcggt taccaccaag atggaagcgt ggtcggtgaa ggagtccttg
     1321 ttggaccggt tctatacgcg gtacagcgat ccgatacaag caaactggac gattgtggat
     1381 attggggcat caattggcga gttttgtgtt gaggctgcga tgcaagcgcc ggatggccgg
     1441 gtaatcgcgt ttgagcctaa tccgggttcg attaatattt tgcgtcaaaa cgtgcgggtt
     1501 aattctctga agaacgtcga gagctataac gtggggattt ggaaggaaaa aggcgagatt
     1561 acgctggaaa ttaatcatga tgagtctatc caggcgcgca ctctggactg cgacaaggac
     1621 gctggaccgg acagccgacg aacgagtatc ccggtcttgt cgctgaacga gatgatgaaa
     1681 ttgattgatg ccgacaagat tgacctgcta aaaattgata ccgaaggcac ggagtttgaa
     1741 atcttgatga gccagccggc agaagtgtta gcgcggattg agcggatgat tcttgaggtg
     1801 cacgagcgtc gacctgagaa gacggtcgcg cagttgggtc agtttttgga gagcatgggt
     1861 tataagacgc gatgtcatga gaattatgtg catgatcatc tgtactattt gtatgcctgg
     1921 cgggagtaga tttttttgcc tgggaccttt ttgagctaat taaggcataa tcgcaacaag
     1981 ctgttaaaat agcaatatgc aggaaaaaaa agaccagcag aatttacgtg aagtaagtga
     2041 atcgcgggaa tttgattcgc gtgtttctga atatttgaag gaaaagcttt gcttttggcg
     2101 aaaagatttt ggcgttgtat caactgatgg tgaggaaagg ttagatgagc aagtttcgtc
     2161 tggacctgct gaaacgcctg acagacaagg acagtggcga caaatcttct ttgctggctt
     2221 tgtttttgtt ctaatcgcgc aaatcatatt tgagtttaaa ctggaaatcc gccatggctg
     2281 ggtattcttt ttgttctttc tggcttggtg actttatttt ttgtgtttta tagcaatgat
     2341 attgctttac ctggttgtgc tgcggtagca agtaaaaagg ctgcgccatt taattatctt
     2401 gccttaaccg gagcggtgct aatcagcgtt ttcagctttt ttttgtggcg gaatagtggg
     2461 tttggcctga tgcaggtttt ggtatggctg gtgagcatcg ctttgcttgg ctatgccagt
     2521 ttctcaggtg gcgaagggaa gcaggcagat gctgaaggtg aaccggtgtt aagtcatcag
     2581 gtcaagtgga aaagttggcg ggaattgttt gcagataaac attatttgac agtctgtgtc
     2641 ctggttattg tgcttgtatt attgtttcaa attcttttca tccgcaaaac gcctgttgag
     2701 ctggtgagtc agcaagtaga gacctttttg gcggtcgaag aaattgcagg cggatccagg
     2761 gcgctgttgt tcccacggga cgtggtcagc gagccactgg gctattactg gctggcattg
     2821 atcagtcgct tattccccgc aagtatgcgg atatgcgctt ttcatgtcgc taatattttg
     2881 agtttttgtt taggcctggc ctttcttttc cgtttggcta aattgctgtt tgataagtgg
     2941 gtggcaatag tggcagtttt tttatttggc gtcggcttct ggcccatatt gcaaaacaca
     3001 gctttgttgg ggtcaagtct cgttttcccc ttgctgtcaa ctgccttgtt ttatcttttt
     3061 aggggtttat tacgtgaaaa acgcggggat atctttctct ttggc
//
LOCUS       DAOSFC010000172         3091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_29793_length_3091_cov_4.994730, whole genome shotgun sequence.
ACCESSION   DAOSFC010000172 DAOSFC010000000
VERSION     DAOSFC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29793_length_3091_cov_4.994730"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PK791_05015"
     CDS             <1..858
                     /locus_tag="PK791_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH92708.1"
                     /translation="NLGFTTEGLRAFPDEPGMHTIDVRVWDYAGNSDEASYTWFVTES
                     IPDNGCLNRNGITKTFQFTKNRFVEDIDVKVMLAHEKRGQIWLQIKGPGDVQPTFVIN
                     SQQDTKKNFNVTINDNSTEPLHNGQDDIIGEPLFGRLAGPSTNNNLAKYFGRSAKGEW
                     TLFVCDNGSGVTGELIDLDLKLYYTNYPPAAGDQVIYIGVGRAVHFILSGMDPDGDEI
                     TFRIIAGPQYGEIETNLPNVTYTPNPGFYGEDSITFVANDGEADSMPATITILVRPMI
                     FFPVITVTD"
     gene            complement(1034..1996)
                     /locus_tag="PK791_05020"
     CDS             complement(1034..1996)
                     /locus_tag="PK791_05020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOH92709.1"
                     /translation="MSSLDRAEARLENLEAIEPLLGSLRVLSLSTMQMALNRQQSLKE
                     YKGRFLEIARLLRSKQKPLKGKKAPKADLEQDIVDDAWDDVQDDDLTIVDGSKPKKRI
                     LAVIGSSRGICGQYNKQLAHQTARLLEKETAGEGEIEVLAFGLRLQNAMRMEAVDFTP
                     GPALFQGSLPDYERVSKLMRSWEAGLSAGKLASVSLLSFKRLGTRNVYKPKLSQLLPD
                     SGHLLDELESQEELFPEPIIEGDPLEILGRIENHLNAIRLYDLILDAISAENLYRYRL
                     LEEAKENTESLVDELSIALQIERRKAITQQMQQLGVASGMLAER"
     gene            complement(1996..2313)
                     /locus_tag="PK791_05025"
     CDS             complement(1996..2313)
                     /locus_tag="PK791_05025"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOH92710.1"
                     /translation="MKMVMTIVPKKFAEETLNAIIEAGFTATFYETRGGMLRQSQTTM
                     FSAVRDEDLVKLLRVIAPIGSDENVLHRGFGIEKQMVFYNGTVVFIWSLEDLPNLSEP
                     ETE"
     gene            complement(2310..>3091)
                     /locus_tag="PK791_05030"
     CDS             complement(2310..>3091)
                     /locus_tag="PK791_05030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003165.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOH92711.1"
                     /translation="LDQGMDVLIVYDDLSKHADTYRELSLLLRRPPGREAYPGDIFYL
                     HSRLLERACRLNEENGGGSITALPIVTTQRGNISSYIVTNLISITDGQIVLNTNDFNK
                     GDKPAIDIGMSVSRVGSAAQESAMKDVVKALKLELAQYEEVARFSRFGTEVSESTQKQ
                     IERGDRLMALLRQGPHQPVRFTEQVLLFYCFSSGHFDTVTPAEIPQFSEALLQHLHVH
                     APQAVDIIRYQRVLSDEAKAQIDEAVQGFDQLWLKKRSKTR"
BASE COUNT          790 a          805 c          709 g          787 t
ORIGIN      
        1 aacctgggct ttaccacgga aggtttacgg gcttttccgg atgagcccgg aatgcataca
       61 attgatgtgc gcgtttggga ttacgccggc aacagcgacg aagcttcata cacctggttt
      121 gttactgaaa gcatcccaga caatggctgc ttgaaccgca atggtatcac caaaacgttt
      181 cagtttacaa agaaccgctt tgttgaagat atcgacgtca aagtgatgct tgcccacgag
      241 aaacgcggac aaatttggct tcaaataaaa ggcccaggag atgttcaacc tacatttgtt
      301 atcaactcac aacaggatac aaagaagaac tttaatgtaa ccatcaacga taacagtact
      361 gaacctttac ataacggtca ggacgatatt ataggagaac cgctttttgg caggctggct
      421 gggccaagca ccaacaacaa ccttgccaag tatttcggtc gttccgccaa aggggaatgg
      481 actcttttcg tctgcgataa tggttccggt gttacaggtg agttaatcga tctggactta
      541 aagctctatt acaccaacta ccctccagcc gcgggtgacc aggtgattta cattggcgtt
      601 gggcgtgctg tccatttcat actgagtggc atggaccccg atggtgacga aatcactttt
      661 cgcatcatcg ctggaccaca atacggtgaa attgaaacaa atttgccaaa cgtgacctac
      721 acaccaaacc cagggtttta tggcgaggac agcatcactt ttgttgccaa cgatggcgag
      781 gcagactcca tgccggccac gataaccatt cttgtgcgtc cgatgatctt tttccccgtc
      841 atcaccgtaa cggattagtt tttgtgctga caaagtatta cttttactga agtataaaaa
      901 atggccggct ttgctggcca ttttttattg tctgactttt tgcttcggcg agacgcctca
      961 gcgaacaggt ttggagttgc gcgtgacacg gattccccgc gccagttata tataaatgca
     1021 atagtttatg gaattagcgc tcagccagca tacccgacgc gacgccaagt tgttgcattt
     1081 gttgcgtgat agctttgcgg cgttctatct gcagcgcgat actcagttca tcaaccagcg
     1141 attcggtgtt ctctttggcc tcttccaaaa gacgatagcg atacaggttt tccgcggaaa
     1201 tagcgtccaa aatgagatca taaagtcgga tagcgttcag gtgattctca atgcgcccca
     1261 gaatctcaag cgggtcgcct tcaataatcg gctccggaaa aagctcttcc tggctttcca
     1321 gctcgtctaa taaatgccca ctatcaggca aaagctggct tagcttgggt ttatacacat
     1381 tgcgcgtacc caggcgttta aagctcaaca gcgaaacaga cgccagctta ccagcgctca
     1441 ggcccgcctc ccacgagcgc atcagtttcg aaacgcgctc gtaatcgggc aaagaaccct
     1501 ggaacaaagc tggtccaggt gtaaagtcca ccgcttccat tcgcatcgcg ttttgcaggc
     1561 gtagcccaaa agccaggact tcaatttcac cttccccggc agtctctttt tctagcaagc
     1621 gtgcggtttg atgcgccagt tgcttgttgt actgcccaca aatcccacga ctgctgccaa
     1681 tgacagccaa aatgcgtttt ttgggttttg acccatcaac tatagtcagg tcgtcatctt
     1741 gaacgtcatc ccaggcatca tcaacaatgt cttgttctaa atctgctttt ggagcttttt
     1801 ttcctttaag gggtttttgt ttgctgcgca acaaacgggc tatttccaag aaacgcccct
     1861 tgtattcctt gagcgattgt tgccggttta acgccatttg catcgtgctc agtgaaagca
     1921 cgcgcaaaga acccagcaaa ggctcaatgg cttccaggtt ttcaagcctg gcttcggcac
     1981 ggtcaagcga actcattact ctgtttcagg ctcggataaa ttgggtaagt cttcaagcga
     2041 ccaaataaag accaccgtgc cattatagaa aaccatttgc ttctcaatac caaaaccccg
     2101 atgaagcacg ttctcgtctg aaccaatcgg cgcgatgacc cgcaaaagtt ttaccaaatc
     2161 ttcgtcccgc acagcactga acatggtggt ttggctctga cgcaacatac caccacgggt
     2221 ttcataaaaa gttgccgtaa aacccgcctc aataatggcg tttaaagttt cttcagcaaa
     2281 ctttttggga acaatcgtca tcaccatttt catcgtgttt tactcctttt ctttaaccat
     2341 aactggtcaa aaccctgcac agcttcgtca atttgcgcct tggcttcgtc actcaagacg
     2401 cgctgatagc gaatgatatc aaccgcctgc ggcgcgtgca catgcagatg ttgcaaaagc
     2461 gcttcggaaa actgtggaat ctctgccggc gttaccgtgt cgaaatgacc actgctaaag
     2521 cagtaaaaga gcagcacctg ctcggtaaac ctgaccggct ggtgtggccc ctggcgcaaa
     2581 agtgccatca aacggtcgcc gcgttcgatt tgtttctggg ttgactcgct cacttccgtg
     2641 ccaaaacgcg agaaccgcgc cacttcttca tattgcgcca attcaagctt gagcgctttg
     2701 acgacatctt tcatggcgct ttcctgcgcg gcagaaccca cacgcgaaac ggacatgccg
     2761 atatcaatcg ccggcttatc gcctttgttg aaatcgttgg tgttcagcac aatttgtccg
     2821 tcagtaatcg aaatcaggtt ggtcacaata taagaagaaa tattgccccg ttgggtggtc
     2881 acgatgggca aggcggtaat gctgccccca ccattttctt cgttaagccg gcaggcgcgt
     2941 tcaagcaaac gggagtgcaa atagaaaata tcacccggat aggcttcacg cccgggcgga
     3001 cggcgcaaga gcaggctcag ctcgcggtaa gtatcagcat gtttggaaag gtcatcatag
     3061 acaatcaaaa cgtccatgcc ctgatccaaa t
//
LOCUS       DAOSFC010000173         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_30633_length_3030_cov_3.971765, whole genome shotgun sequence.
ACCESSION   DAOSFC010000173 DAOSFC010000000
VERSION     DAOSFC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30633_length_3030_cov_3.971765"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..3009
                     /locus_tag="PK791_05035"
     CDS             232..3009
                     /locus_tag="PK791_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOH92712.1"
                     /translation="MITKAEQRRAAKRFSEYWKDKGYEKGESQTFWLTLLRDIFGIKR
                     PEEFVRFEEHVKLDQSNYLDVHIPSTHVIIEQKGKHIDLNKAIRQSDGTLLSPYQQAF
                     QAAAKLPWSKRPRWIVTCNFQEFHIHDMEKPGGEPEIVLLSELENDYYRLFFLVKEDD
                     QNIKKQTEVSLQAGELVGVLYDALHKQYHNPDDPQTLRNLNILCVRLVFCFYAEDSGI
                     FGKHNMFQDYLKPFESNPSMFRKALLDLFRVLDLKTEERAELYLEDDLAAFPYVNGGL
                     FEKDDIVIPFMNSEIIDIILNKASASFNWSKISPTIFGAVFESTLNPESRRAGGMHYT
                     SIENIHKVIDPLFMDALNEEFDEIMAIGAIKERDKKLTQFQKKLASLKFLDPAAGSGN
                     FLTETYLSLRRLENKVIATLRGPQIVYGDAINPIMVSISQFYGIEINDFAVTVARTAL
                     WIAESQMMSETVELIHTDLDFLPLTSNANIVEGNALRMDWNDVVPNTELSYIMGNPPF
                     IGARLMEKEQKEDMEFVFGDFPGLGNLDFVTAWYQKATEYIKDTSIECAFVSTNSITQ
                     GEQVAILWSPLLEKQGIKINFAYTTFVWNSEATDMAHVYCVIIAFSFVEHKEKVLYLS
                     DTQSKKVRNINGYLVDAPNVFVKSRRTPLQEVKPMVFGSMPNDGGNLMLSPDEKKSLV
                     ASYPESEQFIKRFVGAREFINNIERWCLWLEPKDLLLASKIPPIRQRIANVKAIREAS
                     NRASTRELAAYPYRFGEVRQPFGDYLLIPRVSSERRRYIPIGFMRSDIISSDAAIIMP
                     DADLTDFAVLISNVHMGWMRTVAGRLEMRYRYSASIVYNNFPWPNLNDSIKAKLKKTG
                     EQIIESRNLYPNWTLADLYDELTMPPELRKAHQDNDRAVMEAYGFNWRTMTESECVAE
                     LMKMYQDLSKA"
BASE COUNT          929 a          751 c          626 g          724 t
ORIGIN      
        1 aatactcagt gccacggtcg gcgtccgtgg cactcgtgtt taagatttca aattactcaa
       61 agctaattgc acgtggtcag gagaccacat gcgaactttt aatgcttaat tttctccagg
      121 ttcggacggg gtctcctgac cctgtccata gcaaaatcat ttcaataatc aagtcatcga
      181 catctcatta aacgaacccc gtaaatgata taatcactaa caaggtgagc tatgataaca
      241 aaagcagaac aacggcgcgc ggcgaaaaga ttctcggaat actggaaaga caaaggctat
      301 gaaaaaggcg aaagccagac cttttggcta accctgctta gagacatctt tggcatcaaa
      361 aggcccgaag aattcgtccg tttcgaagaa cacgtcaaac tcgaccaaag caactacctt
      421 gacgttcaca ttccctccac ccacgtcatc atcgaacaaa aaggcaaaca cattgacctg
      481 aacaaagcca ttcggcaatc cgatggcact ttgctaagcc cctaccagca agcctttcaa
      541 gccgccgcga aactgccctg gtccaaacgc ccacgctgga ttgtcacctg caacttccag
      601 gaattccata tccacgacat ggaaaaacca ggtggcgagc ccgaaatcgt tttgctaagc
      661 gagcttgaaa acgactacta ccgattattc ttcctggtca aagaagacga ccaaaacatc
      721 aaaaagcaaa ccgaagtctc ccttcaggct ggcgaactgg tcggcgtgct ttacgacgcc
      781 ctccacaagc aatatcacaa ccccgatgac ccccaaacct taagaaacct caacatcttg
      841 tgtgtccgcc tggttttctg tttctatgct gaagattctg gcatctttgg caagcacaat
      901 atgttccagg actacctgaa accattcgaa tcaaacccct ccatgttcag aaaagccctg
      961 ctagaccttt tcagggttct tgaccttaaa actgaagaaa gagccgaact ttacctggaa
     1021 gatgatctgg ccgcttttcc ttatgtcaat ggcggactgt tcgaaaagga cgatatcgtt
     1081 atccccttca tgaactcgga aatcatcgat atcattctca acaaagccag cgcgagtttc
     1141 aactggagca aaatcagccc gaccattttt ggcgcggtat tcgaaagcac actcaacccc
     1201 gaaagccgac gcgcgggtgg catgcactat acgtccattg aaaacatcca caaagttatt
     1261 gacccgcttt ttatggacgc cctaaacgaa gaatttgatg agattatggc aataggcgcc
     1321 ataaaagagc gcgataaaaa actgacacag tttcagaaga aattagcttc tctcaagttt
     1381 ctagaccccg ccgctggctc tggcaatttt ttaaccgaaa cctatttatc gcttcgcaga
     1441 cttgaaaaca aagtcatcgc caccctgcgc ggaccccaaa ttgtttatgg cgacgccatc
     1501 aatcccatta tggtatccat cagccagttt tacggtatcg aaatcaacga ctttgccgtc
     1561 accgtggcac gcaccgcgct ttggattgcc gaatctcaaa tgatgagcga aaccgtagag
     1621 cttattcaca ccgaccttga ctttttgcca ctcaccagca acgccaacat tgttgaaggc
     1681 aacgccctgc gcatggactg gaacgacgtt gtgcccaaca cggaactcag ctacatcatg
     1741 ggcaacccac cttttatcgg tgccagactg atggaaaaag agcaaaaaga agacatggaa
     1801 ttcgtctttg gcgactttcc gggtcttgga aatctggatt tcgtaacagc ctggtaccaa
     1861 aaagcgacgg aatacatcaa agacaccagc attgaatgcg ctttcgtaag cacaaattcg
     1921 attacccaag gtgagcaggt agctatcctg tggagcccac tgcttgagaa acaaggcatc
     1981 aagataaact tcgcctatac aactttcgtt tggaacagcg aagcaacgga tatggcgcat
     2041 gtttattgtg tcatcatcgc tttctcattc gttgaacaca aagaaaaagt gctgtatctt
     2101 tctgacactc aaagtaaaaa agtccgtaac attaatggct acctggttga cgccccaaat
     2161 gtgtttgtta aaagccgaag aacgccactt caagaagtca aaccaatggt ttttggaagt
     2221 atgccaaacg atgggggcaa cttaatgctt tcacctgacg agaaaaaaag tttagttgcc
     2281 agttacccgg aatcagagca atttataaaa agatttgttg gggcaagaga gtttattaac
     2341 aacatcgagc gatggtgttt gtggctagaa ccaaaagatt tattactagc ttctaaaata
     2401 ccccctattc ggcaaagaat agccaacgtt aaagcaatac gagaagccag caacagagca
     2461 agtacacggg aattagccgc ttatccttac cgctttggtg aagttagaca gccttttggt
     2521 gattaccttt tgataccaag ggtctcatca gaaagaagac gctatattcc catcggtttt
     2581 atgagaagtg acattatttc tagtgatgct gcaatcataa tgcctgatgc tgatttaaca
     2641 gattttgcag ttttaatctc aaatgtgcat atgggatgga tgcgcacagt tgctggtaga
     2701 ttagaaatgc gatacagata ttccgcttcc atagtttaca acaacttccc atggcctaac
     2761 ttgaacgata gtataaaagc aaaacttaaa aaaactgggg aacaaataat tgagtcaagg
     2821 aatttatatc caaactggac cttagccgac ctttatgacg aactcaccat gccgcccgaa
     2881 ctgcgcaaag cccaccagga caacgaccgc gcggtaatgg aagcctatgg cttcaactgg
     2941 cgcaccatga ccgaatccga atgcgtcgcc gaattgatga aaatgtacca ggatttaagc
     3001 aaggcttagt gtgatcatat gccttataac
//
LOCUS       DAOSFC010000174         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_30764_length_3020_cov_4.429005, whole genome shotgun sequence.
ACCESSION   DAOSFC010000174 DAOSFC010000000
VERSION     DAOSFC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30764_length_3020_cov_4.429005"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..2763
                     /locus_tag="PK791_05040"
     CDS             247..2763
                     /locus_tag="PK791_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92713.1"
                     /translation="MKYNLVCYQSDNKENPGFFASQPSRGASRQREDDFLVIHLIPAQ
                     NTAFWDSRLAKLTREAAVAFYRSPGSITSAMRDAIECVNKALRVQNSGLRLDQSPESA
                     AMMVGVLRDGALYLAQAAHASALLVSGAGTRLFFDGDIEQRGLGLSDLLELRYYRGDF
                     FGEDYLLMGAPSSLAVIEREAPTPKSVIRLVNEIADLPSAYSLTKISLGEGKVENRLL
                     SLAMLLDEADPPTDTQTGAQIRPAEELADELSPDEVTKADTDLADVVEEKQLDFQEPD
                     AFLVEEDEEPGVEIIEEPVIIDIPQEESGNDTLEFTDPDLVTDTDLAISDIPVQASDI
                     TTDRHDDEPVDFQADISDTIQADTFEENREFVFVHEPEPVDDDIEDEFFSDEPPAPPQ
                     PDLEAIARQERLEKLKKGALTGVARSAGWLRGIEMNAQSFARRTEQKVSASGQEIPSL
                     SPFAKWAIVIIVPLLVVAITVSIYFARGLDRQYVYYINQAHAQIQAAKSSATQGEQRQ
                     ALVQAVVWLNQAREFNQGDTEEIIKMRNEAQGQLDKMDNVRRLMLSNAFGNIVHPDLY
                     ISHLVPGRNAVFALNANTGNILRFYQAGDTYLLDTKFACGPVTIGDVEMGKIIDITDV
                     QGSSPNSANVLGIDAMGNIVLCSDGGQEQIATSLTQPEGGFGSIDAIHYGNDGLYIMD
                     VSQNKIWIYRGLGEVFPYEPGLYLDASDADLTSAIDITVRREGLFVLYADGRTVFSNV
                     PSFSGFVEAQAPNVPWQVAQGNFSQISGLQVADNVLYFLEPGEPSISRYSYRLVPTDV
                     LKVSFGDQATPRQNATAMTVSSSQIVFIAFGSELYFAQLP"
BASE COUNT          738 a          708 c          825 g          749 t
ORIGIN      
        1 gctaaaaatc tcaaattaat gatatcatgc gggtatagga gcgtcaagaa tcggggggcc
       61 ccaggacgtt aatctttcgt taaaaattgt catataaagg atgggtgata tgtatgtttt
      121 acttatgtcg cgattgcttg cgctttgatg cctggttgtt ttaatctttt tataagctgg
      181 catgaatgtt gctcaataat gtatacgact tgtctttgca tttaaggaca gataacaggt
      241 gctattatga aatacaactt agtttgctat cagtcagata ataaagaaaa cccaggcttt
      301 ttcgccagcc agccctcgcg cggtgcttcg cgtcagcggg aagatgactt cctggttatc
      361 cacctgatac ccgcgcagaa cacagctttt tgggattcgc gtttggcaaa gctgacgcgc
      421 gaggcggcag tggcttttta ccgaagtccg ggttcgataa cgagcgcgat gcgcgatgcc
      481 atcgagtgtg tcaacaaggc tttgcgtgtg caaaacagtg gcttacgcct ggaccaaagc
      541 cccgaatcag cggcgatgat ggttggcgtt ttgcgggatg gggccttgta tttggcacag
      601 gcggcacatg ccagcgcttt gttggtgagc ggggcgggca cgcgcctgtt cttcgatggc
      661 gatattgagc aacgcggttt gggtttgagc gatttgttgg aattgcgcta ttatcggggc
      721 gatttctttg gcgaagatta tctcttgatg ggcgcgccaa gcagcctagc tgtgattgag
      781 cgcgaagcac caacaccaaa gtcggtcatt cggctggtga atgagatcgc tgacttgccc
      841 agtgcctatt ccctgacgaa aataagcctg ggcgaaggga aagttgaaaa ccgtctgctt
      901 tctttggcga tgctgctgga cgaagccgat cccccgacag atacccaaac aggagcccag
      961 attagacctg cagaagaact ggctgatgaa ctttctccag acgaagtgac aaaagcggat
     1021 acagacttgg ctgatgtggt tgaagaaaaa cagcttgatt ttcaggaacc cgatgccttt
     1081 cttgtggaag aagatgaaga acccggtgtg gaaattattg aagagcctgt catcattgat
     1141 attcctcagg aagagtctgg aaacgatacg ttagaattta ccgatcctga tcttgttaca
     1201 gatacagacc ttgctataag tgatattcct gtgcaagcaa gcgacataac cacagaccgg
     1261 cacgatgatg aaccagtgga ttttcaggcg gacatcagtg atacaattca agctgatacc
     1321 tttgaagaaa atcgggaatt cgtctttgtg catgaaccag aacccgttga tgatgacatc
     1381 gaagatgaat ttttctccga tgagcccccg gcgccacccc aaccggacct ggaagcgatt
     1441 gcccggcaag agcgccttga aaagctcaaa aaaggcgcgc taaccggcgt tgccagaagc
     1501 gcgggctggt tgcgtggcat tgagatgaac gcccaaagct tcgcgcgccg cacggagcaa
     1561 aaagtgagcg cgtcagggca ggaaatccca tccctgtcgc ccttcgcgaa atgggcgatt
     1621 gtgattatcg tgcccttgct ggtggtggct atcacggtga gcatctactt cgcgcgcgga
     1681 ctggaccgac aatacgtcta ctacatcaac caggcccacg cgcaaatcca ggcagccaag
     1741 tccagcgcca cccagggtga acagcgccag gctttggtgc aggcggttgt ttggctgaac
     1801 caggcccgtg agttcaatca gggtgataca gaagaaatta tcaaaatgcg caacgaagcc
     1861 cagggtcagc tggacaagat ggacaacgtg cgccggctga tgctgagcaa cgcttttgga
     1921 aacatcgtcc atcctgattt atatattagc catttggtac cggggcgaaa tgccgttttt
     1981 gctttgaacg cgaacaccgg caatatcttg cgtttctatc aagccggtga cacctacttg
     2041 ttggacacga agtttgcctg tgggccggtg acaatcggcg atgtggaaat gggcaaaatc
     2101 attgatatca ccgatgtgca aggcagcagt ccaaattcag ctaatgtgct tggtattgac
     2161 gcaatgggca atatcgttct gtgttcagat ggtgggcagg aacaaatcgc gacttctttg
     2221 acccagcccg aaggcggatt tgggagcatc gacgctattc actatggcaa tgatggctta
     2281 tatatcatgg acgtcagtca aaacaagatt tggatctatc gtgggctggg ggaagtcttc
     2341 ccttatgagc ctggcttata tttggatgct tcagatgctg atttgacgtc ggcgattgat
     2401 atcacggtgc gacgcgaagg cctttttgtg ctttacgcgg atggcaggac ggtttttagc
     2461 aatgtcccgt catttagcgg ttttgttgaa gctcaggcgc ctaatgtgcc ctggcaggtg
     2521 gcgcagggta actttagcca gatttccggt ttgcaggtgg ccgataacgt gctgtatttt
     2581 ttggagccag gtgagccttc gatttcgcgc tattcctatc gtttggtgcc taccgatgtg
     2641 ctaaaagtca gttttggcga ccaggcaacc ccacgacaaa acgcgacggc gatgaccgtg
     2701 tcaagttcgc aaattgtttt catcgccttt ggttcggaat tgtacttcgc ccaactgcct
     2761 tagggtgaaa gctatcccaa ctggcgcgtg gcgtctcatg tatcgcgaac actaatatgc
     2821 aaataaatca ccacgcgcgt actaaacctg cacggcggga cgccggcagg agattgtttt
     2881 ttgttcgcgc gtggtctccc gaccacttgc ctaacatggt caggagacca tattagaact
     2941 aagcaggcgc tctcgcccca acgtctcgcc gcgggcatag caaacataga ttctcagcga
     3001 aaaaagcctt ataattgcct
//
LOCUS       DAOSFC010000175         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_30826_length_3016_cov_5.457278, whole genome shotgun sequence.
ACCESSION   DAOSFC010000175 DAOSFC010000000
VERSION     DAOSFC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30826_length_3016_cov_5.457278"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PK791_05045"
     CDS             complement(<1..329)
                     /locus_tag="PK791_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOH92714.1"
                     /translation="MSVQNLLLNWYDTNARELPWRSSKLPYHILVSEFMLQQTQVETV
                     LPYFQRFIETWPTVQDLANAEEQAVLKVWEGLGYYRRAVNLHRSAKMIVQTFGGKVPS
                     TTDALLK"
     gene            complement(332..634)
                     /locus_tag="PK791_05050"
     CDS             complement(332..634)
                     /locus_tag="PK791_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92715.1"
                     /translation="MAVKVVMTWDLKRGNEQEYFEFVVSEYLPKVNQLGLVLTDAWLT
                     AYGERPAMLVGGVLPTVTKARHLLESTGWLDLENELLDFVEDYDVKVFDLKGGFQF"
     gene            complement(636..1286)
                     /locus_tag="PK791_05055"
     CDS             complement(636..1286)
                     /locus_tag="PK791_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH92716.1"
                     /translation="MEITWYGHSCFRFVERGMATVVTDPFDSKVAGYNPLKLKADIVT
                     VSSNDLSHNNVSVIRPEPFVIDGPGEYERGGVFITGIQTNPFTTDKKVMRNTLYVYDY
                     GNMTIMHLGQANNIPPQNAVEELGQINIALVPVGEGGALNASKAAELISMLEPNIVIP
                     MHYATPHSKLALEPLSKFLKEMGITQLETMESYKANASSFTEETRVIALDPKLMAL"
     gene            complement(1404..2162)
                     /gene="recO"
                     /locus_tag="PK791_05060"
     CDS             complement(1404..2162)
                     /gene="recO"
                     /locus_tag="PK791_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOH92717.1"
                     /translation="MSQGGARSYRALAIVLKHREYGEADRILTLYTQEYGKVQAIAKG
                     VRKLKSRKAGHLVPFTLVSLQLAKGRTWELISQAEAQRTFQNIREDLNLTARAAYLVE
                     LIDRFSYEEGPNRGLFQILLDGLQRLDEGFAPTTVTLYFEIRLLDLVGFRPELQRCVA
                     CDAEILPEAQYYSPKLGGALCPRSFSADPTAWRISLNALKYMRHFQRSAWSKIKTLTI
                     PSEIQDEINHLIQHHLTYLLEYNLKTPGFLKSVK"
     gene            complement(2224..2652)
                     /gene="cdd"
                     /locus_tag="PK791_05065"
     CDS             complement(2224..2652)
                     /gene="cdd"
                     /locus_tag="PK791_05065"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOH92718.1"
                     /translation="MVQPNVIDERKLNALKVLASQSAQNAYAPYSRYKVGAAVLVEDG
                     RIYGGCNIENAAYPSGICAERVAIFKAVEAGHRKIIALAVSTANGGSPCGACRQVMRE
                     FLDDDTPIFILNGVGELVEEMKFSDLLPRSFGPEDLQISA"
     gene            complement(2639..>3016)
                     /locus_tag="PK791_05070"
     CDS             complement(2639..>3016)
                     /locus_tag="PK791_05070"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOH92719.1"
                     /translation="EYGGFAGVVTLEDIVEEIVGEIRDEYDTGEEQLYTQISDNEFFI
                     LGRATIDEFNEITGAELSDEYADTLGGYLYGQIGRVPEPGEMVISDGIEFIIEQVQAR
                     RILNVKVRLGAGEEMEDVNYGPA"
BASE COUNT          779 a          792 c          640 g          805 t
ORIGIN      
        1 aatttcaaca aagcatctgt cgttgaaggc actttgccac caaacgtttg cacaatcatt
       61 ttggcacttc ggtgcaaatt tacagcccgc cgataatagc ccaaaccttc ccagaccttc
      121 aaaaccgcct gttcttcggc attagccagg tcttgcacag tcggccaggt ttcaataaag
      181 cgctggaaat atggcaggac tgtttcgacc tgcgtctgtt gcaacataaa ctctgaaaca
      241 agaatatgat aaggcaattt cgaagaacgc caggggagtt ccctggcgtt tgtgtcatac
      301 cagtttaaga gtaagttttg aacagacaca actagaactg aaacccacct tttaggtcaa
      361 atacttttac atcatagtct tcaacgaaat ccagcagctc gttttccaaa tccaaccagc
      421 cagttgattc gagtagatga cgcgccttgg ttaccgtagg caatacaccc ccaaccaaca
      481 ttgccggacg ctcgccataa gctgttaacc atgcgtcagt gagtaccagc cccagctgat
      541 tcactttggg taaatattcg ctaacgacga attcaaagta ttcctgttcg ttgcctcgtt
      601 tgaggtccca ggtcatcaca acttttactg ccattttata acgccattaa tttggggtcc
      661 aaagcaatta cacgggtttc ttcagtgaat gacgacgcgt ttgccttgta tgactccatt
      721 gtctccagtt gggtaatgcc catttccttt aggaatttgc tcaaaggctc aagtgccagc
      781 tttgagtgag gcgttgcata gtgcattgga ataacgatat tcggctcaag catgctaatc
      841 agttcagcgg cttttgaagc gttcaaggcg ccaccctcgc caacgggcac cagggcaata
      901 ttgatttgtc ccagttcctc aactgcattt tggggcggta tgttattagc ctgtccaaga
      961 tgcatgatag tcatattgcc ataatcatat acatacaagg tattgcgcat tactttttta
     1021 tcggtagtga atggattagt ttggataccc gtgataaaaa cgcccccgcg ttcatactca
     1081 cccgggccat caataacaaa tggctcaggg cgaataacac tcacgttatt gtgtgaaaga
     1141 tcgttggaac taaccgtgac aatatcagct ttcaacttta aggggttata gcccgcaact
     1201 ttcgagtcga atgggtccgt aaccacagtt gccataccgc gttcaacaaa tcgaaaacaa
     1261 gaatgtccgt accaagtaat ttccattagt cctccgtgcc tcgttctcgt tcgtgaggcg
     1321 tctttattat atcagctgaa caaaactgac gaaagattat cgctcattct gtccgtgctt
     1381 caacattcga ttatgctttg cctttatttg acggatttca aaaagcctgg cgttttgaga
     1441 ttgtactcca acagataagt cagatgatgc tgaatgagat ggtttatctc gtcttgtatc
     1501 tccgatggaa tagtcagggt ttttatcttt gaccacgcag aacgctgaaa atgacgcata
     1561 tacttgagtg cattaagcga aatgcgccac gcggtcggat ccgcagaaaa gctcctgggg
     1621 cataacgccc cacccagctt gggcgagtaa tattgcgctt cgggcaatat ctccgcatcg
     1681 caagctacac aacgttgcag ttccggtcga aagccaacta ggtcaagcag acgtatttca
     1741 aaatacagtg tcaccgttgt tggcgcgaag ccttcatcca gacgctgaag cccatccagc
     1801 aatatctgaa aaagcccacg atttgggcct tcttcgtagc taaagcgatc gattaactcg
     1861 acaagatatg cagcccgcgc cgtaaggttc aggtcttcgc ggatgttttg aaaagtgcgt
     1921 tgcgcttctg cctgagatat caattcccag gtgcgccctt ttgccagctg taatgaaacc
     1981 agggtaaaag gtaccagatg tccggctttt cggcttttca gcttgcgcac acctttagca
     2041 atcgcctgaa ctttaccgta ttcctgggtg taaagcgtta aaatgcggtc tgcctcaccg
     2101 tattcacgat gcttaagtac gattgccagc gcgcgatacg agcgcgcccc accttgcgac
     2161 acgcctaaat cctttccgcc cgctctgtca tgccaagctt caccgtttca acgatatgca
     2221 gtatcaggcg gatatctgca aatcttcggg gccaaacgaa cgtggcaaga gatcagaaaa
     2281 cttcatttcc tcaacaagct cacccacgcc attcaaaata aagatcggcg tatcgtcatc
     2341 aagaaattcg cgcattacct gccggcaggc accacaaggc gaacccccgt tcgctgtgct
     2401 caccgcaagc gcgataatct tacgatgtcc cgcttccact gccttgaata tcgctacccg
     2461 ttcggcacaa atgcccgaag gataggcggc attttcgata ttgcaaccgc catagatgcg
     2521 cccatcttcc accagcaccg ctgcgccaac cttataacgc gaatacggtg cataggcgtt
     2581 ttgcgcgctt tggcttgcta aaacttttag cgcgtttagt tttctttcat caatcacatt
     2641 aggctggacc atagttcaca tcttccattt cttcgcctgc accaaggcgc acttttacgt
     2701 tgagaattct tcgggcctga acctgctcaa tgataaattc aatgccatca ctgatgacca
     2761 tttcgccggg ctctggcaca cggccaattt gaccatacag gtagccaccc aaagtatcgg
     2821 catattcatc agaaagttcc gcgccagtaa tttcgttgaa ctcgtcgata gtagcacgcc
     2881 ccaggatgaa aaattcatta tcgcttattt gtgtgtagag ttgctcttca ccagtatcat
     2941 attcatcgcg aatttcgcct acgatttctt caacgatatc ttctagcgtt accacccccg
     3001 cgaaaccgcc atattc
//
LOCUS       DAOSFC010000176         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_30964_length_3007_cov_4.260501, whole genome shotgun sequence.
ACCESSION   DAOSFC010000176 DAOSFC010000000
VERSION     DAOSFC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30964_length_3007_cov_4.260501"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PK791_05075"
     CDS             <1..188
                     /locus_tag="PK791_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92720.1"
                     /translation="HLARKYNGQWIPAASPVLPFNLSGWIPVDGESPYEGWLVRGNDT
                     VRASVLSDAESMVPAEE"
     gene            complement(334..1953)
                     /locus_tag="PK791_05080"
     CDS             complement(334..1953)
                     /locus_tag="PK791_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOH92721.1"
                     /translation="MTKHNEETTGNLSGADLSSEELLPSKKKNKKKPFYKEPVLLAVV
                     GLLLVAGILLGIWLRKPKDDLSKTPDIATATHDHQATPFVKKETSIPTATTAPTEVPE
                     VVMATEEPRTEVITYTIQEGDLLTEIAERFGIWPDTIIWANRYEHGTDVLEYNAGDTI
                     FILPVNGAYHKWYQGEGLNGVAKYYNVTPDDIIDFPGNNLNRDTLGSLSNPNITPGTR
                     LVIPGGTLPDYFASDALLYHAAVQKLRSLPLGDAESYPEPRAEIIAYEVREKDTLFSI
                     AEDFNLEPETILWANRYIIGDTPDGIYPGQKLIILPEDGVYHAWSYGEGLNAVSRTYG
                     VTPEDVLAEPMNEIDEESIGDFALPKIATGSFLYIPGGKGMTPSWVSYVPTNDPSVKR
                     ADVSYLGPYACQSMLVLKGTGAWQLPTPSSYISGYEYNPPVHNGLDYGGTIGTPLFAA
                     DSGVVIYAGWSDRGYGNTIVIDHLNGYISLYAHIMHGGFNVGCGQAVPAGTTIGYMGS
                     TGNSTGPHLHFEIRYNGSPVNPPPQLWALAD"
     gene            complement(2045..>3007)
                     /locus_tag="PK791_05085"
     CDS             complement(2045..>3007)
                     /locus_tag="PK791_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOH92722.1"
                     /translation="LNLSDWYPLIPPYDPETGWIAYNRVTDHNNIIIGEYVVQETADF
                     NVSLKLINQPEFIEVAASAPATMIDDTYYYELPLARAFAFSVSDSYFEHEIVHNGTRI
                     HTYLFMNHQEYGPDLCEIARKALELFEELFYPYPREMISIVAGDFLHNMEMDGMFMLS
                     HKVIDNYHSGEKNNMTILTPHELSHQWFYSLVGNDSVKEPWLDETLATYMEVLYFERY
                     HPETVDWWWRNRVYEYDHSGYVNSDIHIDGGYEKYRAAVYLNGARFMADLRATVGDEA
                     FFAALKEYAYENTWRIATREDFFRAFANHSNANLSGLLHQYFRD"
BASE COUNT          746 a          672 c          783 g          806 t
ORIGIN      
        1 tgcatttggc gcgaaaatat aatggacagt ggatacctgc tgcttcgcct gttttaccct
       61 tcaatctttc tggatggatt ccagttgacg gtgaaagccc atacgaaggc tggctggtgc
      121 gaggtaatga caccgtgcgg gcgagtgttc tttcggatgc ggagagcatg gttccggcgg
      181 aagagtaatt ttttaacgca tgacagggat gtcatgtttc agttgatggt ttttatattg
      241 atgagaaaaa ccggtagatt ctttgaatga aaaagagatg cggtcaaacg catctctttt
      301 agtgtcttct gtaagcaaat tcaggctatt gccttaatct gctaaagccc aaagctgtgg
      361 ggggggattg actggcgagc cattgtagcg gatttcaaaa tgcaggtggg gaccggtgga
      421 attgcctgta ctgcccatat agccgatggt ggtacccgca ggaacagctt gcccgcagcc
      481 aacattaaag ccaccatgca taatgtgcgc gtagaggctg atatagccat tcaagtggtc
      541 gatcacgatg gtattgccat aaccgcggtc agaccaaccc gcgtaaataa caacaccact
      601 gtctgccgca aacaagggcg tgccaattgt tccaccgtag tcgagtccat tatgcacggg
      661 tggattgtac tcatagccgg aaatatacga actcggtgta ggcaattgcc aggcaccagt
      721 tccttttaag acgagcatgc tttggcaagc gtagggtccc aaataagata catcagcgcg
      781 tttcacactg ggatcatttg ttggcacata agatacccag ctgggagtca tgcctttgcc
      841 accagggatg tatagaaaac tgcccgtagc aattttgggc aaggcaaaat cgccgatact
      901 ttcttcgtca atctcattca tcggttcagc gagaacatct tcgggcgtaa cgccataagt
      961 ccgcgaaacc gcatttaatc cttcgccata ggaccaggcg tggtaaacgc catcttccgg
     1021 caaaatgatg agtttttgtc ctgggtaaat accgtccggt gtatcaccga taatatagcg
     1081 gtttgcccaa agtatcgttt cgggttccag gttgaagtcc tctgcaatac taaaaagggt
     1141 gtctttttcg cgtacttcgt aagcgataat ctccgcgcgg ggttccggat agctttcagc
     1201 atcgcctaaa ggcaaggaac gcagtttttg caccgcggcg tggtagagca gggcgtcgct
     1261 ggcaaagtaa tcgggcagtg tgccacccgg aatgaccagt ctggtgcctg gtgtgatgtt
     1321 ggggtttgaa agcgacccca atgtatcgcg gttgagattg ttgcctggga aatcgataat
     1381 atcatcaggc gtgacgttgt aatacttggc aacgccgttg aggccttcgc cttggtacca
     1441 tttgtgataa gcgccattta caggcaagat gaaaatcgta tcgccggcgt tatattccag
     1501 cacgtctgta ccatgttcgt aacggtttgc ccagatgatg gtgtcgggcc agatgccaaa
     1561 gcgttcagcg atttcggtaa gcaaatcgcc ttcctggatc gtatatgtaa ttacttccgt
     1621 gcgcggttct tcggtagcca tgaccacttc gggaacttcg gtaggcgcgg ttgtcgcggt
     1681 ggggatgctc gtttcttttt tgacgaaagg tgttgcctga tggtcatgcg tggctgtggc
     1741 gatatctggc gttttgctca agtcatcttt tggcttgcga agccaaatgc caagcaatat
     1801 accagccacc agcaaaagac caacaactgc caggagtaca ggctctttgt agaaaggttt
     1861 tttcttgttt ttctttttag atggcaacag ttcttcgctg gataaatcag ctccagacaa
     1921 attgccagtc gtttcttcgt tgtgcttagt catactaact ccttatgaat cgttgctaaa
     1981 ttccaacttt gtaagttaca actaactatg tattatatac catttggaaa attccatgaa
     2041 tcagctaatc acgaaagtat tgatgaagaa gtccgctgag attagcattg ctgtggttgg
     2101 caaaggctct aaagaagtcc tcgcgcgtcg cgatgcgcca ggtgttctca taagcgtact
     2161 cttttaatgc agcaaagaaa gcctcatcgc caacagtggc gcgcaaatct gccatgaagc
     2221 gggcaccatt tagatatact gccgcgcggt acttttcgta acccccgtca atgtggatat
     2281 cggagttcac gtagccactg tggtcgtatt cgtaaacgcg gttgcgccac caccaatcaa
     2341 cggtttcggg gtgatagcgt tcgaagtaaa gcacttccat atatgtggcc agggtttcgt
     2401 caagccaggg ctctttgacg gagtcattgc caaccaaact gtaaaaccac tgatgcgaaa
     2461 gctcatgcgg tgtgagaatg gtcatgttat ttttctcgcc actgtgataa ttatcaatca
     2521 ccttgtggct aagcatgaac atgccatcca tttccatatt atgcaaaaag tcaccggcaa
     2581 caatagaaat catttcgcgt gggtagggat agaaaagttc ttcaaacagt tcgagagctt
     2641 tacgggcgat ttcgcaaagg tctggtccgt attcctggtg gttcatgaac aaataagtgt
     2701 gaatgcgcgt gccgttgtga acgatttcat gttcaaaata gctgtcgctg accgaaaagg
     2761 caaaagcgcg agccaggggc agttcgtaat agtaagtgtc gtcgatcatc gtggctggcg
     2821 cgctggcggc gacctcaatg aattctggct gattgatgag ttttagactg acattgaaat
     2881 ctgctgtttc ctggacgacg tattcgccga taataatgtt gttatggtcg gtaacccggt
     2941 tataggcaat ccagcctgtt tccggatcgt aaggcgggat gagtggatac cagtcagaca
     3001 ggttgag
//
LOCUS       DAOSFC010000177         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_31070_length_2999_cov_6.953804, whole genome shotgun sequence.
ACCESSION   DAOSFC010000177 DAOSFC010000000
VERSION     DAOSFC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31070_length_2999_cov_6.953804"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="PK791_05090"
     CDS             <1..1171
                     /locus_tag="PK791_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92723.1"
                     /translation="NVEDLGESCVLAKPMSITQEGYTITLEALSSTDKKSWVRFSLDG
                     WQGDRDFYYMMIREFGKLQYNIDTIKPQSTFVGDIYQSELLYLEYELPPVCHAKDFRL
                     AFLSLPASKQNMAPEVWEFEPKLRAAQKEDTLPKNDQGFLSSEEHEGITMQILAYYET
                     PKFTGLSYRFQTPSLRNSVSLLGGWNPSNPMIVDSEGNNYYEQDRRSASSHFGYFSMV
                     NKMPHDESFHLNSPSMMLYWNELDFEILENKAPITIDFSANFEKGHEWKLDFWYEYDY
                     PHKIYFDKVVYRESPPHLEVHYGTDNDINYIQLYCFSDVAPLCKGCPYSSTEGVFRIG
                     LAEKPSGQLKLGLYSVSREIEPPEGWTIDFTLDELPKAEWLPLYDVPYIVPEQRP"
     gene            complement(1258..2358)
                     /locus_tag="PK791_05095"
     CDS             complement(1258..2358)
                     /locus_tag="PK791_05095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOH92724.1"
                     /translation="MSEHPLASWLDQTVQGDCLEILPTLPAESVDLIFADPPYNLQLQ
                     GELLRPNLSLVQGVDEDWDKFESLKAYDEFTTAWLSACRRVLKPTGTLWVIGSYHNIY
                     RVGSILQDLDYWILNDVVWVKSNPMPNFRGVRFTNAHETLIWAQKEKGARYTFNYQAM
                     KMLNDDLQMRSDWFIPICTGKERLREGGKRLHPTQKPEALLYRILMATTNPGDVVLDP
                     FFGTGTTGMMARKLSRHYIGIEIDPRYVYHARERLSKNVQQEFDAPLLITPNPRSRKR
                     IPFGLLVEQGYIMPGTRLSLGDEGHIWATVQADGALVVGRQRGSIHRLAREILGRPAN
                     GWMLWYYWDESTQSKCKIDEIRKKYREDIEES"
     gene            2534..2989
                     /locus_tag="PK791_05100"
     CDS             2534..2989
                     /locus_tag="PK791_05100"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOH92725.1"
                     /translation="MTSTINPDWQDFKFYQEIKPRYGDFDTQLHVNNLAYLEMGESVR
                     IDYYKDVGIWDGVIRPGGFGMLMASIKTDFVNSINYGDPVHIGYKTARIGNKSITFAF
                     IIKNPQSNLVYARGEVVGVCYDHFAGKSVRVPQEWRQKLSEYENNKDLL"
BASE COUNT          808 a          709 c          707 g          775 t
ORIGIN      
        1 caacgtggaa gacctaggcg aaagctgcgt gctggcgaag ccgatgagca ttacccagga
       61 gggttacacc atcacgcttg aagcgcttag ctcaacggat aaaaaaagct gggtgcggtt
      121 ttcgttggat ggctggcaag gtgaccgcga tttttattac atgatgatac gcgaatttgg
      181 caagttgcaa tataacatcg ataccatcaa accccagtca acatttgttg gagatattta
      241 ccagtccgag cttttatatc tggaatatga attgccgcct gtttgccacg ccaaagattt
      301 caggctcgct tttcttagct tgcctgcatc aaagcaaaat atggcaccag aagtatggga
      361 atttgaaccc aaactacgcg ccgcgcaaaa agaagatacc ctgcccaaaa atgaccaggg
      421 atttctaagc agcgaagagc acgaaggcat taccatgcaa atactggctt actatgaaac
      481 ccccaaattc acaggcttat cttaccgttt tcagacacca tcgctcagga atagcgtgtc
      541 tctcctgggc ggatggaatc ccagcaaccc catgattgtt gattccgaag gtaacaacta
      601 ctatgagcag gatagaaggt ccgcaagctc gcattttggc tatttctcaa tggtgaacaa
      661 aatgccacac gatgaaagct tccacttaaa cagcccttca atgatgttgt attggaatga
      721 gctggatttt gaaatactcg aaaataaggc gcccattaca attgacttta gtgctaattt
      781 tgaaaaggga catgaatgga aactggattt ctggtatgaa tatgactatc cccataaaat
      841 atattttgat aaagtggtct atcgcgaaag cccaccgcat cttgaagtcc actatgggac
      901 ggataacgac attaattata ttcaattgta ttgcttttct gatgtcgcgc ccttgtgcaa
      961 agggtgtcct tacagcagta ccgaaggtgt tttccggatt gggctggcag aaaaaccaag
     1021 cggtcagttg aagctgggac tttatagtgt tagcagagaa atagagccac ctgaaggctg
     1081 gacaatagac tttacgctgg acgaactgcc caaagccgag tggctgccct tatacgatgt
     1141 gccttacatc gtgccagaac aacgtccgta gagcatgttt taatgcgcaa gctattataa
     1201 aaaagcaaaa acccctttaa ttcgtaaggg gttttgtttt ttaacaatta atgcggttta
     1261 agactcttca atatcttcgc ggtatttttt gcgaatttca tcaattttgc actttgattg
     1321 cgtgctttcg tcccagtaat accaaagcat ccagccattc gcgggacggc cgagaatctc
     1381 acgcgccagg cgatgaattg atccacgttg cctgccaacc accaaagcgc catcggcttg
     1441 cacggttgcc caaatatgcc cctcgtcgcc caaactcaag cgggtaccag gcataatgta
     1501 accttgctca accaacagac caaaaggaat ccgtttgcgt gatcgcggat taggcgtgat
     1561 tagaagtggc gcgtcaaact cctgttgcac gttcttgctc aagcgttcgc gcgcgtgata
     1621 aacataacgc ggatcaattt cgatgccgat gtaatgccgg cttagctttc gcgccatcat
     1681 gcctgtggtt cctgtgccaa agaaggggtc cagcaccaca tcgccagggt tagtcgttgc
     1741 catcaaaatc cgatacagca aggcttcggg tttttgggtg ggatgcaagc gcttgccacc
     1801 ttcacgcagg cgttctttgc ctgtgcagat ggggataaac cagtccgagc gcatttgcag
     1861 gtcatcgttg agcatcttca tggcttgata attgaaggtg tagcgcgcgc ctttttcttt
     1921 ttgcgcccag atgagtgttt cgtgcgcgtt cgtaaaccgc acaccacgga aatttggcat
     1981 ggggttactc ttgacccaaa ccacgtcatt gagaatccaa tagtctaaat cctgcaggat
     2041 gctccccaca cggtagatat tatgatacga gccaatcacc cacaaggtgc ctgttggttt
     2101 gagcacgcgc cgacaagcgc tgagccacgc ggtcgtgaat tcatcatatg ccttgagact
     2161 ttcgaactta tcccagtctt cgtccacgcc ttgcaccagg ctaaggttcg ggcgtaaaag
     2221 ctcaccttgc agttgcaagt tgtagggcgg atccgcgaaa atcaggtcta ccgattcagc
     2281 cggcagggtt gggagtatct cgagacaatc gccctgaact gtctggtcaa gccaggaagc
     2341 tagggggtgt tcgctcatag tctaattgta atggattttg aacatatttg gtggatttca
     2401 ttaacgtaaa tgatgcatat caaggtgtaa agattgtctg tttcacagaa acagcagcga
     2461 tggtatctgg cgttttctgt aaaaccttct tgtatttgct ttgaacaaag ctataatcga
     2521 tggggaggca aatatgacat caaccattaa ccccgactgg caggatttta agttttacca
     2581 agagattaag ccccgctatg gcgattttga cacccaactg cacgtcaaca acctggctta
     2641 tcttgagatg ggcgaaagtg tgcgcatcga ctactacaag gatgtgggca tttgggatgg
     2701 tgttatccgc cctggtggct ttggtatgct gatggcgtct attaaaactg actttgtcaa
     2761 ttccatcaat tacggtgacc ctgtccacat cggttataaa actgcccgca tcggcaacaa
     2821 aagcatcacc ttcgccttca tcatcaaaaa cccacaaagc aatctcgtct atgcgcgcgg
     2881 agaagtggtg ggtgtttgct atgatcattt cgccggaaaa tctgtgcgcg ttccccagga
     2941 atggcgccaa aaattatcag aatacgaaaa caacaaggat ttactatgac caaacccac
//
LOCUS       DAOSFC010000178         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_31074_length_2999_cov_4.735734, whole genome shotgun sequence.
ACCESSION   DAOSFC010000178 DAOSFC010000000
VERSION     DAOSFC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31074_length_2999_cov_4.735734"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PK791_05105"
     CDS             <1..904
                     /locus_tag="PK791_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92726.1"
                     /translation="LFAFIMAIAFVISNPFLFTLKGIASYARTLYSEFSQISAGYNLV
                     YEKGFAATWAGIQPAYGTLPFLIAVAFSFALGFLDKDKQKLSLLLLCWFVPLTVTVLF
                     ATHFKYQYWLPAAIPAFSMLYMLIPDKTWKKDKLWLNIPRAVFVAVVVLQVVFFNIQN
                     VQSVSAQIQREENSESIQFYREAIQVLEPTRNESLNVYYDYRVYIPFDSEPWQLHTAF
                     EMLDYPYIQEENFDILLLMNQRVTDYLNPRAEGVNPDKLETARIFYREVRDNQVRNYV
                     LLYEDDFGKTFIRQDLFEQYYQNP"
     gene            complement(978..2690)
                     /locus_tag="PK791_05110"
     CDS             complement(978..2690)
                     /locus_tag="PK791_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOH92727.1"
                     /translation="MQKQDYFFTDNPRDSVELSLDDGRVITGPRNGRLEAFAKVAQKE
                     QEAMIVGVVLDGVLRELTYKLERDGKGSFITMADEDGARIYRRSLTFLLELAFKRCFA
                     NGLLTIDYSVSSGGYYCQVDNLPEFGPENLAQLEATMRQLVKEDLPFVRDTVPLEEAV
                     AYFTECGEEDKVRLLKYRNRPDLVLYSLGEVRDYHHGYMVPSTGYLKWFALEWVGDAF
                     IMHFPRRHKPNGLSPMTKYPKLLKAFQDYGEWLSKLGIPSVGALNDAIQMGHIDEVIL
                     VSEALHEMQISNIADQIQERHDKAGLVLIAGPSSSGKTTFSKRLGIQLLARGITPFAL
                     EMDNFFIDREKTPLTPEGEKDFESLRALDLKRLNKDLRALLTGKKVQLPRYDFLSGLS
                     VDGETAQLKKDDIIILEGIHGMNPDLLEGIGAEETFKIYVSCLTQLNLDRHNRISTTD
                     TRLLRRILRDSRDRGYSAEETISRWEQVRKGETQYIFPYQENADAMFNSALAYELSAL
                     KPLVEPLLRQVRFGTREHIEAKRLLKFLEWFLPISIEYVPSNSLLREFTGDSLLTNFS
                     VWEH"
     gene            complement(2705..>2999)
                     /locus_tag="PK791_05115"
     CDS             complement(2705..>2999)
                     /locus_tag="PK791_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92728.1"
                     /translation="RTGNYLPIQSNCRSLYVVLREQDGKQMLVIANLKDDTVENCSLT
                     IKESTLQGEYTAEMLMGAPLFESISFEENGALTEWSIARAIEAAETFIIILNP"
BASE COUNT          811 a          755 c          627 g          806 t
ORIGIN      
        1 acttttcgcc ttcatcatgg caatcgcctt tgtcatttcg aacccgtttt tgttcaccct
       61 gaaaggcatt gccagctatg cccgcacctt atatagtgaa ttctcgcaaa tctctgctgg
      121 ctataatttg gtctacgaaa aaggttttgc agcaacctgg gcaggcattc aacccgctta
      181 tggcacctta cccttcctga ttgccgttgc tttttccttt gccttaggat ttttggacaa
      241 agacaagcaa aaattatcgc tgttactgct ctgttggttc gttccgttga ctgtcacggt
      301 tttgttcgcc acacatttca agtaccaata ctggttgcct gccgccattc ccgccttctc
      361 gatgctttat atgcttatcc ctgacaaaac atggaaaaaa gacaaacttt ggctcaacat
      421 cccccgcgcg gtttttgtag ctgtcgttgt tttacaagtc gttttcttca acatccaaaa
      481 cgttcaatcc gtaagtgccc aaattcagcg ggaagaaaac agcgaaagca tccaattcta
      541 ccgcgaagcc atccaggttt tggaaccaac ccgaaatgaa agcctgaatg tgtactatga
      601 ctaccgtgtt tatatcccct ttgacagtga gccctggcag ctccataccg catttgaaat
      661 gctcgattac ccgtatatcc aggaagaaaa ttttgacatt ttgttgctca tgaatcaacg
      721 cgtcacagac tatctcaatc cgagagcgga aggtgtcaat cctgacaaac tcgaaacagc
      781 gcgcatattt tatcgcgaag tgcgtgataa ccaggttaga aattacgtcc tgctttacga
      841 agatgatttc ggaaaaactt tcatacgcca ggatttattc gaacaatact accaaaaccc
      901 ttaagctaaa gttaaattta atacaaagac gctggtgtgt gattagcacc agcgttttat
      961 ttgcaatcaa ttcagaatta atgttcccaa accgagaaat ttgtcagcag ggaatcgcct
     1021 gtaaactcgc gcaaaagtga gttagaaggc acatactcaa ttgaaatcgg caggaaccac
     1081 tccaggaact ttagcaagcg tttcgcttca atatgttccc gcgttccaaa acgtacctgt
     1141 cgcaaaagcg gttccaccag gggttttaag gcgcttagct cataagccag ggcggaattg
     1201 aacatcgcat cagcattttc ctgatatggg aagatgtatt gggtttcgcc cttccgtacc
     1261 tgttcccatc gagagatagt ttcttccgct gaataccctc tgtcacgcga atcgcgcagg
     1321 atgcgtcgca acaagcgggt atcggtcgtg gatatgcggt tatgtcggtc aaggttgagc
     1381 tgggttaagc aagacacata aatcttgaag gtttcttccg ctccgatgcc ttcaagcaaa
     1441 tcggggttca taccatgaat accctcaaga atgatgatat cgtccttttt cagttgggcg
     1501 gtctcgccgt ccacgctcaa gccactcaaa aagtcataac gcggtagttg tactttctta
     1561 cccgtcagca aagcccgcaa gtctttattc aagcgtttaa ggtctaaagc gcgcaaggat
     1621 tcaaaatcct tttcgccttc aggcgtcagt ggcgttttct cgcggtcaat gaagaagtta
     1681 tccatttcca gggcaaaagg cgttatgcct cgcgctaaaa gctgaatacc caaccgtttt
     1741 gagaaagtgg tttttcccga tgatgaagga ccggcaatca gaaccaaacc cgctttgtca
     1801 tgtcgctctt gaatttggtc cgcgatgttt gaaatttgca tctcatgcaa ggcttcagag
     1861 accaaaatga cttcgtcaat atgccccatc tggatagcgt cattcaaggc gcccacagat
     1921 ggaatgccca gtttactcag ccattcgcca taatcctgaa aagccttgag taatttcggg
     1981 tacttggtca ttggggagag cccgttgggt ttatgccgcc gcggaaaatg catgataaag
     2041 gcatcaccga cccactccaa agcaaaccat ttcaggtagc ccgtcgatgg caccatataa
     2101 ccatgatgat agtcacgcac ttcgcccagg ctatacaaca caaggtctgg gcggttgcgg
     2161 tactttagta agcgcacctt atcttcctcg ccacattcag taaaataggc gacagcttct
     2221 tccaggggca ccgtgtcgcg cacaaagggt aaatcttctt taaccagctg acgcattgtg
     2281 gcctcaagtt gagcaagatt ttccggccca aattcgggca gattatcaac ctgacaataa
     2341 tagccaccag aagaaacgga ataatcaatc gtcagcaaac catttgcaaa acaacgctta
     2401 aaagccagtt caagcaaaaa ggttagcgaa cggcgataaa tacgcgcgcc atcttcatca
     2461 gccatggtaa taaaactccc cttgccatcg cgttcaagct tgtaggttaa ttcgcgtaaa
     2521 acaccgtcta aaacaacgcc aacaatcatg gcttcctgct ctttttgggc cactttagcg
     2581 aatgcttcca gtctgccatt gcgtgggccg gtaatgaccc tgccatcatc caaacttaac
     2641 tcaacgctat cgcgtggatt gtctgtaaaa aagtaatctt gtttttgcat taattctcca
     2701 tcaattatgg attcaaaata ataataaagg tttctgcagc ctcgattgct cgcgcgatgg
     2761 accattcggt caaagcgcca ttctcttcaa agctaatgct ctcaaacaga ggcgcaccca
     2821 tcagcatttc cgcggtatat tcaccctgaa gggtactttc tttaatcgtc aagctacagt
     2881 tttcaacagt atcgtctttc agattagcaa taaccagcat ttgtttgccg tcctgttcgc
     2941 gcaagacgac atagagtgaa cgacaattag attgaatcgg taaatagttc cccgtgcgc
//
LOCUS       DAOSFC010000179         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_31088_length_2998_cov_5.252463, whole genome shotgun sequence.
ACCESSION   DAOSFC010000179 DAOSFC010000000
VERSION     DAOSFC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31088_length_2998_cov_5.252463"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /gene="glgC"
                     /locus_tag="PK791_05120"
     CDS             complement(<1..1159)
                     /gene="glgC"
                     /locus_tag="PK791_05120"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOH92729.1"
                     /translation="MPSRDDIIAVILGGGRGTRLFPLTSERAKPAVPIAGKYRLIDIP
                     ISNCINSGIYRISVLTQFLSVSLHRHITRTYVFDSFHQGGVQIWAAEQSPITTDWYQG
                     TADAVRKQIQEIQSTRAKYVLILAGDHLYRMDYGKMAQYHWDHGADVTVAVQPVPKDE
                     AFRFGILKQDKNGEITRFAEKPKDPNILADLVSLDDPAMPYLGSMGIYMFNTDVLVDV
                     LQKEIDGKLFVDFGKDIIPYLISAESPVFGYQYEGYWRDIGTIRTFYQTNLELTEDEP
                     EIDFYDPEKPIYTHARFLPGTRAIGCKLSRALLAEGCTIREAEIDHCVIGLRQQIGEG
                     SVIKDTIMMGADWYGVQRNGAPLGIGKNCNIQGAIIDKNAAIGDNVVIKPFP"
     gene            complement(1273..>2998)
                     /gene="recN"
                     /locus_tag="PK791_05125"
     CDS             complement(1273..>2998)
                     /gene="recN"
                     /locus_tag="PK791_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOH92730.1"
                     /translation="ELHIQNFAIIEKLSLFFQSGLIVFTGETGAGKSIILDSLSAILG
                     ERMDTTVIRQGENKAIVEGLFELDVQTKSALRELLEPEGLWDDEDYLTLGREIRAEGR
                     TIARVNGRMVNLGLQSEIGALLVDLHGQSEHLSLLKTRNHRILLDRYAHNKDLLEAYQ
                     AEYKRWQALNEKLRELNAIDNMASERIDILRYQIQEIESAKLQPGEDEELRSERTRLA
                     NVEALSTFSQQALVAIDEGSSESSAATDLVGQAAQSLHDLARIDASTQALAERAEEAL
                     SSLADIAYELRDYLEAIEYNPVRLDQVEERLNTLQILKKKHGGSIESALAHLDKIKRE
                     IENIDTLDEQIKEVSEKINSVKLSLSEKALSLSKLRKSSAEKMQEAVSNELNALQMQK
                     ARFMVQIDFSPSDDGLLVDNLRLAFDQNGIDQIEFLIETNPGEGFKPLAKIASGGETS
                     RLMLALKHVLAEADQTPTLVFDEIDSGIGGRVGMTVGKMLWQLGREHQVLCVTHLPQL
                     AAFADQHYKASKHESAGRTLTNVHELDTAQRERELANMLGGETQSILQSAREILATVS
                     EFTGTIHS"
BASE COUNT          747 a          754 c          683 g          814 t
ORIGIN      
        1 aggggaatgg tttgatgaca acgttatcgc caatcgcagc atttttatcg ataatcgcgc
       61 cttgaatgtt acagtttttg ccgattccca agggcgcgcc gttgcgctgt acgccatacc
      121 agtccgcacc catcataatc gtgtctttta ttactgagcc ctcgccaatt tgctggcgta
      181 aaccaatgac gcaatggtca atttctgcct cgcgaatggt gcagccttca gccaacaagg
      241 cgcgtgacag tttgcagcca atggcgcgtg tgcctggcag aaaacgagcg tgggtgtaaa
      301 tcggcttttc gggatcgtaa aaatcgatct cgggttcatc ttcagttaat tcaagattcg
      361 tttgataaaa cgttcgtatc gtaccgatat cacgccaata gccctcatac tgatagccaa
      421 acacaggcga ttcagcagag attaaatagg gaatgatatc tttgccgaag tcaacaaaca
      481 attttccatc aatttccttt tgcaaaacgt ccacaaggac atccgtattg aacatgtaaa
      541 ttcccatgga acccagataa ggcattgcgg gatcatcgag actgaccaaa tccgcaagga
      601 tattagggtc tttgggtttt tcagcaaagc gcgtgatttc accattcttg tcttgtttta
      661 agatgccaaa gcggaaagct tcgtctttgg gtacgggttg cacagcaacg gttacatcag
      721 cgccatggtc ccagtgatat tgtgccatct tgccataatc catgcgataa agatggtcgc
      781 ctgccaggat gagtacatat ttggcacgcg tgctttgaat ttcctggatt tgcttgcgca
      841 cagcgtctgc tgtaccttga taccagtcag tggtaattgg agactgctca gcagcccaaa
      901 tttgtactcc accctggtga aaagagtcaa aaacatacgt tcgcgtgata tgccgatgca
      961 aagacacgct caaaaactgg gtcaacacag aaatccggta aataccagag ttgatacagt
     1021 tgctaatggg aatatcaata agccgatact ttccggcgat aggcacggcg ggttttgcgc
     1081 gctcggaagt gagagggaac aaacgtgtac cccgaccacc acccaagata accgcaataa
     1141 tatcgtcacg actgggcata gtagcttcct ttctatcttg tgtgtgataa aacgcccctt
     1201 tgggcatctt aatcacgaca ataaattttt aatttttcaa tacgggtgat ttgcacccga
     1261 aaacaatatg gtttatgaat gaattgtacc tgtaaactct gaaactgtcg ctagaatctc
     1321 cctggcggac tgtaaaatgc tttgtgtttc gccaccaagc atattggcca gttcgcgttc
     1381 acgctgggca gtgtctagct cgtgtacatt ggtcagtgtt ctgccagcac tttcatgttt
     1441 actggctttg taatgctggt ccgcgaaggc ggccagctgg ggtaagtggg taacgcaaag
     1501 cacctgatgc tcacggccca gctgccaaag cattttccca accgtcatgc ccacccgtcc
     1561 gccaatgcca ctgtcaattt catcaaagac aagtgtgggg gtttggtctg cctcggcaag
     1621 tacatgttta agggcaagca ttaggcgtga agtctcaccg ccggatgcaa tttttgccaa
     1681 aggtttaaag ccttcgccag gattggtttc aatgaggaat tcaatttggt caataccgtt
     1741 ttggtcaaaa gccaaacgga gattatcaac aagcagccca tcgtcactgg ggctgaaatc
     1801 aatctgcacc ataaaacgtg ctttttgcat ttgaagcgca ttgagttcat tgctaaccgc
     1861 ttcttgcatc ttctcagcgc tggatttgcg tagcttgcta agacttagtg ctttttcaga
     1921 aagacttaac ttaactgaat ttattttttc gctgacttct tttatctgct catccagggt
     1981 gtcgatattt tctatctcgc gctttatttt gtccagatgc gccaaagcac tttcaatcga
     2041 cccgccatgt ttctttttta gaatctgcaa ggtgttgagc cgctcttcaa cctgatccaa
     2101 ccgcaccggg ttgtactcaa tcgcttccag ataatcgcgc agttcataag caatatccgc
     2161 cagactactc aaggcttctt cggcccgctc agccaaagcc tgggtgctcg cgtcaatgcg
     2221 cgccaaatca tgcaaagact gcgctgcctg accgaccaaa tccgtcgcgg cactgctttc
     2281 cgaagaccct tcgtcaatcg caaccaaggc ttgctgacta aatgtactta aagcttccac
     2341 gtttgccagt cgggtgcgct cggaacgcag ctcttcatct tcaccgggct gcagtttggc
     2401 gctctcaatt tcctgaattt gataccgtaa gatatcaatg cgctcagagg ccatgttgtc
     2461 aatagcgttc agctcgcgta atttttcatt taaggcttgc cagcgtttgt attcagcctg
     2521 atacgcttca agcaggtctt tattgtgcgc gtagcggtcc agcagaatgc ggtgattgcg
     2581 tgttttgagt aagctgagat gctcagactg cccatgcaag tcaaccaaaa gagcgcctat
     2641 ttccgattgc aagcccaggt tgaccatacg cccgttcaca cgcgcgatgg tacgcccttc
     2701 cgcgcggatt tcccgcccga gagtcagata atcttcatca tcccacaagc cttcgggctc
     2761 aagcaattca cgcaatgcgc ttttggtctg tacgtcaagt tcaaaaagcc cttcaacgat
     2821 cgccttgttt tcgccctggc ggataacggt ggtatccatg cgttctccga gaattgcact
     2881 tagcgagtca aggataatcg acttgcccgc gccagtttcg cccgtaaaaa cgattaaacc
     2941 cgattgaaaa aacaaactga gtttttcgat aatggcgaag ttctggatat gcaattct
//
LOCUS       DAOSFC010000180         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_32091_length_2934_cov_4.672456, whole genome shotgun sequence.
ACCESSION   DAOSFC010000180 DAOSFC010000000
VERSION     DAOSFC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32091_length_2934_cov_4.672456"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PK791_05130"
     CDS             complement(<1..930)
                     /locus_tag="PK791_05130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOH92731.1"
                     /translation="MNYPQAVQEPKQRKSTWMDTAQLVVSILVVLVFFGISILSFVGV
                     AVSGTLRELREAIPSFWTMGFVFLFLAGIALVSAIKSRQVYYDKTKAQQPKKDTKWIY
                     FSIAFLPPLLVAGYFVINAPRMPAFLLPLNTVLALSVAMLWILKLGLRDSWGANVKRD
                     SGLFSFSMSFSTFYILLLEIFVFVIVVAITMATVFKNIDLGAMLNNLTLNPETYLEDM
                     SFDGNFVLLVLFVITISGPIIEELFKTIGVWFLKPRHISPREGWIAGLMSGAGFGLIE
                     SLMFGAVQGVLMTNFPDWLYFVLGRAGALVLHTF"
     gene            complement(933..2303)
                     /locus_tag="PK791_05135"
     CDS             complement(933..2303)
                     /locus_tag="PK791_05135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH92732.1"
                     /translation="MKKISKLLLVLLMGALLLSACKGTVPVPENMSTQASKEETDEPI
                     EIELTDPAVESELGSKSAEDIFALTRTSTDPLTTDDNRMACTPGEPLFAPLSDEIRVQ
                     YQIPDIPEVSEEDWVLGSEDALMTIIFYTDFQCPYCALFASESHKLLETYPDDVRLVY
                     RPLPLSFHNLAPLAAQAAEAAGLQGKFWEMHDHIFAKQQDWNSLTIEGFTEWLNDAAE
                     ELGLDVEQFSADVVSEDVVIKITEQTEFHLSLGINATPSLFINGYPWQQSWYASTLGL
                     VVDTMKAEAKLSFECPPFVIDPEKTYIATMETEKGKMVFELYPNVAPLAVNSFVYLSQ
                     SGFYDGVTFHRVLQDFMAQGGDPTGSGMSGPGYTYAEETHPELLFDQPYMLAVAKTNA
                     PSSSGSQFFITFEPAEWLNGGYTIMGRLIEGVDVLKSITLRDPSAQPAPDFEGDKIIR
                     ITIEEN"
     gene            complement(2363..>2934)
                     /gene="tmk"
                     /locus_tag="PK791_05140"
     CDS             complement(2363..>2934)
                     /gene="tmk"
                     /locus_tag="PK791_05140"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOH92733.1"
                     /translation="PALAQYLEAQGYRVLCTREPGGTSIGDQIRAVLTSMDNPELLPR
                     TEILLFLAARAQLVAQVIRPALREGKVVLCDRYGDSTLAYQGYGHGLDLNSLRAMLNF
                     ATEGLKPDLTLLMDLDIKTGLARKKSIDEWNRLDAYEVSFHERVRAGYLQLAEEEPER
                     WQRVDASMPKDEVQTMLRQIVLQRLKDKI"
BASE COUNT          818 a          701 c          680 g          735 t
ORIGIN      
        1 aaaagtgtgc aggactaaag cgcccgctct gccaagcaca aagtaaagcc aatctggaaa
       61 gttcgtcatc aacacacctt gtactgcccc aaacataaga gattcaatga gtccaaagcc
      121 cgcgccactc attaatcccg caatccaacc ttcacgcggg ctgatatggc gaggtttaag
      181 gaaccaaacc ccaatcgttt taaaaagttc ttcaatgatc ggtccggaaa tggtgatcac
      241 aaaaagaaca agcagaacaa agttaccatc aaacgacatg tcttcaaggt acgtttcggg
      301 attcagggtt aagttattca acattgcgcc aagatcgatg ttcttaaaaa cagttgccat
      361 agttattgca acaacaataa caaagacaaa tatttccaga agaagaatat agaaagtact
      421 aaagctcatg ctgaaactaa agagcccgct atcgcgctta acgttcgcgc cccaggaatc
      481 gcgcaatccg agttttagaa tccaaagcat ggcgacggat aatgccagga cggtattcaa
      541 tgggagcaaa aacgcaggca tacgcggcgc gttaataaca aagtatcctg ctacgagcag
      601 cggaggcaaa aatgcgatgc taaaatagat ccattttgtg tctttcttag gctgttgtgc
      661 tttggtttta tcataataga cctggcgaga cttgatagcc gataccaaag ctattccagc
      721 taaaaacaaa aaaacaaacc ccatcgtcca aaaggagggg atagcctctc ttaattctct
      781 cagtgttcca cttacggcaa cgccgacaaa acttaagatc gaaattccaa aaaagacaag
      841 aacaacaaga atggacacca ccagttgggc ggtatccatc catgttgatt ttctttgctt
      901 aggctcttgt acagcttgtg ggtaattcac ggctaatttt cttcgattgt gattctgata
      961 atcttatcac cctcaaaatc aggtgcgggc tgggcggaag gatcgcgcag tgtgatgctc
     1021 ttcagcacat ctacgccttc aatcaatcgt cccataatcg tatagccacc gttaagccat
     1081 tcggcaggtt caaaggtgat aaagaactga ctgccactgc ttgaaggtgc attggtcttt
     1141 gctacagcca acatataggg ttggtcaaac aaaagctcgg ggtgcgtttc ttccgcatag
     1201 gtgtatccag ggccagacat acctgaacca gttgggtcgc ctccttgtgc catgaagtct
     1261 tgcagcacac ggtggaaagt tacgccatca taaaagcccg attgtgagag atacacgaag
     1321 gagttaaccg ccaaaggcgc gacgttgggg taaagttcaa aaaccatctt acccttttca
     1381 gtttccatcg tggcgatgta cgttttttcc gggtcgataa caaagggtgg gcattcaaac
     1441 gaaagttttg cctcagcttt catcgtgtca actaccagtc caagggttga ggcataccag
     1501 ctttgttgcc agggatagcc attgataaac aaagacgggg tagcgttgat gcctagactc
     1561 agatgaaatt cggtttgttc ggttatttta ataactacat cttcactgac gacatcagca
     1621 ctgaattgtt ccacatccaa accaagttct tcggcggcat cgtttaacca ctcggtaaaa
     1681 ccttcaatgg tcaaactatt ccagtcttgt tgttttgcaa agatatggtc atgcatttcc
     1741 cagaacttgc cttgcagacc agcagcttcg gcagcctgtg cggcaagggg agctaaatta
     1801 tggaatgaca aaggcaaagg gcgataaacc aggcgtacgt catcgggata agtttcaagc
     1861 aacttgtgag attcgctggc aaaaagcgcg cagtaaggac attggaaatc ggtatagaaa
     1921 atgatggtca ttaacgcatc ttctgaaccc aaaacccagt cttcttcact gacttcagga
     1981 atgtcaggaa tttgatattg cacgcggatc tcatcagaca aaggcgcgaa cagcggttca
     2041 ccaggcgtac aagccatgcg attgtcatca gttgtcagcg gatcagtgct cgtgcgcgtt
     2101 agcgcaaaga tgtcctcagc tgatttactg cccagttctg attctacggc agggtcagta
     2161 agttcgattt cgattggttc gtcggtttct tctttggaag cttgggtaga catgttctcg
     2221 ggaacgggca ctgtgccttt gcacgcgcta agcaataaag cacccatcaa gagaaccagc
     2281 aaaagttttg atattttttt catatgatcc tttcatccgt ccaggcaaac gcctttgtcg
     2341 gttcatttac aatagtagct aactatattt tatcctttaa tcgttgcaat acaatctggc
     2401 gtaacatggt ttgcacctca tcttttggca ttgaagcgtc cacccgttgc cagcgttcag
     2461 gttcttcttc agcaagttgc aaatatccag cgcggacacg ctcgtggaac gagacttcat
     2521 aggcatccag gcggttccat tcgtcaatgc ttttcttgcg tgccaaacct gttttgatat
     2581 ccaagtccat gagcagggtc aggtcgggtt tgagtccctc ggtagcaaag ttgagcatcg
     2641 ctcgcaagct gttcaaatcg agaccatgtc catagccctg ataagccagg gtcgagtcac
     2701 cgtagcggtc acatagcacc actttaccct cgcgcaaagc cggtcggatg acctgcgcca
     2761 ccaactgcgc gcgggctgcc aaaaaaagca ggatttctgt gcggggcaag agttccgggt
     2821 tgtccatgct ggttaatacc gcgcggattt ggtcgccaat tgatgtgcca ccaggttcgc
     2881 gcgtgcaaag cacacgataa ccttgcgctt caagatattg cgctaacgcg ggga
//
LOCUS       DAOSFC010000181         2913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_32450_length_2913_cov_5.194192, whole genome shotgun sequence.
ACCESSION   DAOSFC010000181 DAOSFC010000000
VERSION     DAOSFC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32450_length_2913_cov_5.194192"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..621)
                     /locus_tag="PK791_05145"
     CDS             complement(1..621)
                     /locus_tag="PK791_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOH92734.1"
                     /translation="MSKKDNNYFKMFIDIAADAITAATHLKETINNYDPEKIHEQMLA
                     MHEVEHAADNKKHQMMEKLIAEFITPIEREDIITLSWALDDVLDYMEEIYQILFMHNV
                     KSMREEALVFGDLIYQSTVALKTCFDEFEHFKRSKILPKRLIEVNAIEEKADKLYIDA
                     VRRLHLDESDPSKVHTWSVLFLQLERTIDRIEQVADTIELVIMKNS"
     gene            complement(631..1674)
                     /locus_tag="PK791_05150"
     CDS             complement(631..1674)
                     /locus_tag="PK791_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOH92735.1"
                     /translation="METLQSLFSNPALIITTILTIGVILVNGWTDAPNAIATAIGTRA
                     MKPRSAIIMAAVFNFLGVFVMTMINTNVVVTISNMVDFGGDPHAASVALMGALASIVI
                     WATAAWYFGIPTSESHALIAGLTGSAVAFQGSFAGVNGAEWIKVIAGLLISTVMGFGL
                     GWGITKLIEMLFKNVDRRKTIGFFRNLQIAGAASMAFMHGAQDGQKFMGVFLIGVYLS
                     QGKAVPPSFTIPFWMIIVTSVIMAIGTSIGGQRIIKAVGLDMVKLETYQGASADIAAS
                     ISLLFASLFKGMPVSTTHTKTTAIMGVGASRRIRSVNWAIVKEMVLAWVLTFPGCGLL
                     GYLVTKLLMLILV"
     gene            complement(1845..2159)
                     /locus_tag="PK791_05155"
     CDS             complement(1845..2159)
                     /locus_tag="PK791_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH92736.1"
                     /translation="MPVYAYKCDACGHEFDKFQHFVDDPLTVCPVCAEEALRKVYSPV
                     GIVFKGKGFYATDNRSPSGISSSKPSNNSSANGSSDTSSSDTAKASASTGSDKKSSTV
                     SA"
     gene            2379..>2913
                     /locus_tag="PK791_05160"
     CDS             2379..>2913
                     /locus_tag="PK791_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92737.1"
                     /translation="MSKAKNTIKNKLKTKSPRRKPWIYTGILLGLIVSALLAYQLPPI
                     RNRVDTLYSKLYYKFNPPSASVFSPSQQGTVEAIVKQTEAAMASIASEVPTAQVSEET
                     PLVTDEATQTPTAETSTPYPVPASYTIAGMGLEYQTFNNCGPANVSMNLNFWGWETNQ
                     EKMRLALRTHDHDRNVML"
BASE COUNT          799 a          688 c          659 g          767 t
ORIGIN      
        1 ttaagaattc ttcataatca ccaattcaat cgtatcagcg acttgttcaa tacggtcgat
       61 ggtgcgttca agctgcaaga ataaaactga ccaggtatgt acttttgacg ggtctgattc
      121 atcaaggtgt aagcgacgta cagcgtcaat atagagtttg tcggcttttt cttctatcgc
      181 gtttacttca atgagacgtt taggcaatat ctttgaacgt ttgaagtgtt caaattcatc
      241 aaagcaagtt ttcagggcaa ccgtactttg ataaatcaag tccccaaaaa caagggcttc
      301 ttcgcgcatg cttttcacgt tgtgcatgaa caatatctga taaatctctt ccatatagtc
      361 cagcacatcg tccagcgccc acgacagcgt gatgatatct tcgcgttcga tgggtgtgat
      421 gaactccgcg attagctttt ccatcatctg atgttttttg ttatccgcgg cgtgttcaac
      481 ttcatgcatc gcaagcattt gttcatgaat cttttcggga tcgtagttgt tgatggtttc
      541 ttttagatgt gttgcagcag taattgcgtc tgccgcgata tctataaaca tcttgaagta
      601 gttgttatct ttttttgaca tattattcct ttacactaag atgagcatga gtaattttgt
      661 gaccaaatag cccaacaacc cacagccagg gaaggtgagc acccaggcca gtaccatttc
      721 tttaacaatt gcccagttca ccgagcgaat gcgtcttgaa gcaccaacgc ccatgatggc
      781 ggttgttttg gtgtgcgtgg tggaaaccgg catacctttg aacaaggaag caaataaaag
      841 agagattgag gcagcaatat cagcactggc gccctgatag gtttccagtt tgaccatatc
      901 cagtccaacc gctttgatga tgcgctgccc gccaatcgac gtgcctattg ccatgatgac
      961 cgaagtaacg attatcatcc aaaaaggtat tgtgaacgac ggaggcacag ctttgccctg
     1021 gcttaggtaa acgccgatca aaaatacgcc cataaacttc tgtccatctt gcgcgccatg
     1081 cataaaggcc atcgatgccg cgccagcaat ttgcagattg cggaagaaac cgatagtttt
     1141 acgccggtca acgtttttga agagcatctc aataagtttg gtaattcccc agcccagtcc
     1201 aaaacccatc acggttgaaa tcagcaatcc agcgatgacc tttatccact ccgccccatt
     1261 tacgcccgcg aaactaccct gaaacgccac cgcggaaccg gttaaaccgg caatcagcgc
     1321 gtgactttcg ctggtgggga tgccaaaata ccaggcagct gtcgcccaga tgacgatgga
     1381 tgccagcgcg cccatcaagg cgacagacgc ggcgtggggg tctccgccaa agtcgaccat
     1441 gttggagatg gtgaccacca cgttggtgtt tatcatagtc attacaaaaa cgcccaggaa
     1501 gttgaagacg gcagccatga tgatagcact gcgtggcttc atggcgcgcg tgccgatggc
     1561 ggtggcaatg gcgttgggcg cgtcagtcca gccgttgact aatatgacac cgatggttaa
     1621 tatggttgta atgatgaggg cgggattaga gaatagtgat tgtagcgttt ccaatgtctg
     1681 cacctttgat gttgtcctgg tgattgtaaa ctatatttta caaataatgt ggaaaacgac
     1741 acgaaatata aagtattctt aataaattga gggtgaaaac aagcggagcg cagctagtgc
     1801 gcttgtttgt ttatacagat tgatttcgat gagcttttag cgaattacgc tgatactgtg
     1861 cttgattttt tatcagaacc cgtgctggca cttgcttttg cggtatcaga cgaactggta
     1921 tctgatgagc cattcgctga tgaattgtta cttggcttac tggagcttat gccactgggc
     1981 gagcggttat cggtcgcata gaaacctttt cctttaaata caatacctac cggagaataa
     2041 accttacgca aggcttcttc cgcacaaact gggcagacag tgagcgggtc atcaacaaaa
     2101 tgttgaaatt tatcaaactc atgtccgcag gcatcgcatt tataagcata tactggcata
     2161 tctatcaaaa ctcctattac aatccacaat agtaccatag atttaacaag atttccgccc
     2221 cgcttaaggc tttttgatga aacgctaaca atgaaaaaga ctttggtatt atctcctttg
     2281 agcctgggcg tgctttaaag tatcattaat gatagaagtc tgcatgagct gaacctgaag
     2341 actttggcgt tgaattttaa tttagatcga gaaatgcgat gagcaaagca aaaaacacaa
     2401 taaaaaacaa gctgaaaacg aaaagcccca ggcgcaagcc ctggatttac actggcatct
     2461 tgcttggcct gattgtttcg gcattactgg catatcagtt gccacccatt cgtaaccgcg
     2521 ttgataccct ttattccaag ctttattaca aattcaatcc accatccgca tcggttttta
     2581 gcccgagtca gcaaggcacg gttgaagcca tagtcaagca aaccgaagct gctatggcat
     2641 ccatagcaag cgaagtccca accgcccaag taagcgaaga aacgccctta gtcacagacg
     2701 aagctaccca aacgcccact gcggaaacct ccacgcccta tcctgtgccc gcgtcctata
     2761 cgattgcagg catgggcctt gaatatcaaa ctttcaacaa ctgcggtcct gccaacgtgt
     2821 ccatgaacct gaacttttgg ggctgggaaa cgaatcagga gaaaatgcgc ctggcactgc
     2881 gcacacatga ccacgaccgc aatgtgatgc ttg
//
LOCUS       DAOSFC010000182         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_32944_length_2883_cov_5.576379, whole genome shotgun sequence.
ACCESSION   DAOSFC010000182 DAOSFC010000000
VERSION     DAOSFC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32944_length_2883_cov_5.576379"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PK791_05165"
     CDS             complement(<1..1529)
                     /locus_tag="PK791_05165"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOH92738.1"
                     /translation="MYPTTVTVNITAVLETLNQILTAGVTITTVALFMYALGFNWRDR
                     IAQTFGLILLSVALIYSSETIAAISEQDHIIQFWLRIKWTGLVMLPAVYLHFSDVVLT
                     LTGRPSRGRRRKVVYAVYIISIIMTILVWFGITVGGLATIKAPMFYLERNQVTYWFGW
                     FYLLVMFLASTNLIRATRRSITSTSRRRLIYLLTGAAIPVLTSILFLFHGNTFLAAHP
                     NLFWMMSIFFAALTIPTLVMMAYVVSFFGLTWTDRAIKSRLLRWLLRGPFVVALVLGL
                     TTLVRRYGLSQGDPYNQYIPVVMVSSTLVLQYLITVLSPKLEQKLFWGDEKGDFEIIQ
                     GLQDRMLTKKDLNQFLETIVASICDRVQSPGCFVVAYDAGEVELIVSAGNKRVLSHVP
                     MEASKLEEIKQAITPEKAFLEWGDIYLVPLLDDVDNDDQQILGLCGFMRPKGTMDEEA
                     EYDVRLMTERANLALHDRHLQKHVIDSIATLQSEVDYIQNLRASTSYDRRGLLESGVR
                     EV"
     gene            complement(1533..2072)
                     /locus_tag="PK791_05170"
     CDS             complement(1533..2072)
                     /locus_tag="PK791_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH92739.1"
                     /translation="MAKLNIFTANDSYYDEMLALEHFVPSRHAYKLEIRNEENSSHFS
                     FQRIRLPREVNLAYSRDEAELIPHWKQADLVFVATVEEHLMGYVVLDVRHLPKTVRVT
                     DLVVNQDARRCGIASAMVSVCETWAQTNKYNRVVLEIPLRNDPMVRLAQKLGYAMNGF
                     MDQYFPNHDPAVFFEKRIG"
     gene            complement(2119..2820)
                     /locus_tag="PK791_05175"
     CDS             complement(2119..2820)
                     /locus_tag="PK791_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92740.1"
                     /translation="MDSVIRFLAEFEVPIYIILGIVAVVFLRRIVLAFEEKKKAVFGL
                     EREAAQRNIVQAATVLILVGLLTAGEFVVATFLAGELDQAPTYATATIDKLKTPTVTL
                     DPALIEVPEDATPTPTRYPQAEVEGLDTNCVAGVLEITSPQHNDHTKGVVRFEGTVNT
                     ESLGSYQFDYSTMGEPNWQTISAGNGVKIEESIGNWYTSELLPGPYLVRLTALDNKGV
                     QSDVCIIVIYVDAND"
BASE COUNT          757 a          803 c          654 g          669 t
ORIGIN      
        1 acttcacgca cgcccgactc cagcaatccg cgccggtcat aggacgtgct ggcacgcaga
       61 ttttgaatat agtccacctc tgattgcaga gtggcaattg aatcaatgac gtgtttttgc
      121 agatgacggt catgcaatgc caggttagcc cgttcggtca tcaagcgcac atcgtactcc
      181 gcttcttcgt ccattgtccc ttttgggcgc ataaagccac acaaacccag gatttgctgg
      241 tcatcattat ccacatcatc caacaaaggc accaggtaaa tatctcccca ttccaaaaag
      301 gccttttccg gcgtaattgc ttgtttgatt tcttccagtt tgctcgcttc catcggcaca
      361 tggcttagca cgcgtttgtt tccagcagaa acgattaact ccacctcacc agcgtcataa
      421 gccaccacaa aacaccccgg actttgcacc cgatcgcaaa tactggcaac aatcgtctcc
      481 agaaactgat tcaaatcctt tttggtcaac atgcggtcct gaagaccctg gataatctcg
      541 aaatcgcctt tttcatcgcc ccaaaagagt ttttgctcca gctttggcga cagcacggta
      601 atcaaatact gtaaaacaag tgttgaagat accatcacca ccggaatata ttgattatag
      661 gggtcgccct ggctcaaacc atagcgacgc accagcgtgg taagccctaa gacaagcgct
      721 acaacaaatg ggccccgcaa cagccagcgc aaaaggcggc ttttgatggc gcggtcagtc
      781 catgtcagac caaaaaacga gaccacatag gccatcatta ccagcgtagg aatagtcagt
      841 gccgcgaaga aaatggacat catccaaaac aaattcggat gcgccgcgag aaaagtgttg
      901 ccatggaaca ggaagagaat actcgtcaaa acaggaatag ccgcgcctgt aagcaaataa
      961 attagccgtc gccgactggt actggtgata ctgcgtcgtg tcgcgcggat gaggttggta
     1021 ctcgccaaaa acatcaccaa cagataaaac cagccaaacc agtaggtcac ctgattgcgc
     1081 tcaaggtaga acatcggcgc ttttatcgtc gccaaaccgc ccaccgtgat gccaaaccac
     1141 accaaaatcg tcatgatgat gctgatgata tacaccgcgt agaccacttt gcgtcgccgt
     1201 ccgcggctgg gtctgcccgt caaggtcagc accacgtccg agaaatgcaa ataaacagcc
     1261 ggcaacatca ccagccccgt ccacttgatg cgcagccaaa actgaatgat atggtcttgt
     1321 tcagaaatcg ccgcgatcgt ctcgctggaa taaatcaacg ccaccgataa aaggatgagt
     1381 ccaaaagtct gcgcgatacg gtcgcgccag ttgaagccca gagcatacat aaacaaggcc
     1441 acggtggtga tcgttacccc ggcggtcaga atctgattaa gcgtttccag caccgcggtg
     1501 atattaacag ttacagttgt aggatacaaa gcttatccaa tccttttctc aaaaaagacc
     1561 gctgggtcat gattcgggaa atactggtcc ataaaaccgt tcatcgcgta acccagtttt
     1621 tgcgccaaac gcaccatcgg gtcgttgcgc aaggggattt ccaaaaccac acggttgtac
     1681 ttgttcgtct gcgcccaggt ttcgcacacc gacaccatcg cgctggcaat cccgcaccgc
     1741 cgcgcgtcct ggttcaccac caaatcggtg acccgcacgg tctttggcag atggcgcaca
     1801 tcaagcacca cataacccat caggtgttct tccaccgttg ccacaaaaac caagtcagct
     1861 tgtttccagt gcgggataag ctcggcttca tcgcgtgaat aagccaaatt gacttcacgt
     1921 ggcagacgta tgcgctgaaa gctgaaatgc gaactgtttt cttcgttacg gatttccagc
     1981 ttgtaggcat ggcgactggg cacaaaatgt tccagtgcca gcatctcgtc atagtaagag
     2041 tcgtttgcag taaaaatgtt tagttttgcc ataattgtct tttcaggtgc ttaaatttct
     2101 gtcgcggttc aaagtcgcct agtcgttcgc gtcaacgtaa ataacgataa tacacacatc
     2161 cgattgtacc cctttattgt ccagtgcggt taagcgcacg agatacggtc cgggcaagag
     2221 ttcgctggtg taccagttac cgatgctttc ttcaatttta acgccgttgc ccgccgaaat
     2281 tgtttgccag ttcggttcgc ccatcgtgga atagtcaaac tgatacgacc ccaaactttc
     2341 cgtattcacc gtgccttcaa agcgtaccac ccctttggta tggtcgttgt gctgtgggct
     2401 ggtaatctcc aaaacgcccg ccacgcagtt ggtgtcaagc ccttcgactt cagcctgcgg
     2461 ataacgcgtt ggtgttggcg tggcatcttc cggcacttca atcagagcgg ggtcaagcgt
     2521 tactgttggc gttttcagct tatcaatcgt cgcggtagcg tatgtcggcg cctggtcaag
     2581 ttctccggct aaaaaagtcg ccaccacgaa ctcacccgcg gtcaaaagtc cgaccaggat
     2641 aagtactgtg gcagcctgaa cgatattgcg ttgcgcggct tcgcgttcca gcccaaaaac
     2701 agctttcttc ttttcttcga acgccaaaac aatccggcgc aaaaaaacga ccgcgacaat
     2761 acccaaaata atgtaaatgg gtacttcaaa ttcagccaga aagcgaatga ctgagtccat
     2821 tttcctcgcg cgaagggcaa attacattcg cccaaccacc gtccggacga ttttcatcag
     2881 ctt
//
LOCUS       DAOSFC010000183         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_33115_length_2872_cov_5.408946, whole genome shotgun sequence.
ACCESSION   DAOSFC010000183 DAOSFC010000000
VERSION     DAOSFC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33115_length_2872_cov_5.408946"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PK791_05180"
     CDS             complement(<1..273)
                     /locus_tag="PK791_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOH92741.1"
                     /translation="MSKIIQKVKGTRDFYPQEMAQRQWLIEKLRVASEAFGYQQYDGP
                     CLEQIDLYAAKSGEELVKEQAFVFEDRGGDPITLRPELTPTVSRMVA"
     gene            435..1514
                     /locus_tag="PK791_05185"
     CDS             435..1514
                     /locus_tag="PK791_05185"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH92742.1"
                     /translation="MKYKLLAIIITVMLVLTACGPKETVKPTDSVSAFPVVVDSSQDT
                     YPAGDAVDTATEDTYPIAETIAQDIYPVSSGEAISITVLAPSSTTSIPILLAAKYLPG
                     MTVEIFDNHPQAHAQFMAEDNLVLVTGLSVGVDMFKNDVPLQLINTNVTGLSYLVSCN
                     LEINSFADLAGHEIYLPFEGSPLEETTRFFVEQAGLQYGVDVKPIYAPFDTSTALIKN
                     GDDAIVAMPQPAATAMAAQECGHLGASFYDTWNAITGDTDGYPQVGAFAKAEFASAHP
                     EWVQAFNQAVAKAIADLQANPEAAVNEVADKFSFPAPVLLQALQATRFTVQSDQSMKD
                     SVIHYYVTIGKPLDESYEAFFYALP"
     gene            1477..2238
                     /locus_tag="PK791_05190"
     CDS             1477..2238
                     /locus_tag="PK791_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOH92743.1"
                     /translation="MKATRRSSTPFLNELIGFGLLIILWAVLSLFFPVYIIPSPIEVF
                     KATPEFLNAQFFSAMGITLLRIVVGFAISFFIGTGLATLFAGTKLSEQLSAMVQAFQV
                     IPAVTVGVILVIIFGVGSAPPIILITLMTLPLVFMNTLQALKFPDPKLLEYLDSIKAD
                     RKLRFSALMKPQIIKVTRTNLILGFSMASKIAVTGEFIGSQDGLGYLLNKARLVFAMK
                     EVFFYIFFFIVLTVLFQGLVELIYATSLRKYTYGD"
     gene            2228..>2872
                     /locus_tag="PK791_05195"
     CDS             2228..>2872
                     /locus_tag="PK791_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH92744.1"
                     /translation="MATNTQQNVVLEANSISKQFDNEMVISNFSFKLLQNERLLLFAP
                     SGAGKTTLLRILCGLLEADSGSFSFPGGAEPHIVFQEPRLFPHMTVDENIKLPLKSPH
                     FASKPNALDLLPEWYQLSGLHEYQGHYPHTLSGGMKRKVSLLRALITLPELLILDEPF
                     QSLDKKSREIFVHSIFKNIPKLSVLFTSHDILDVEITATTILLIKSPKLQNPIII"
BASE COUNT          732 a          769 c          577 g          794 t
ORIGIN      
        1 tgccaccata cgcgagaccg ttggcgttaa ttccggacgc aaagtgatag ggtcaccacc
       61 ccggtcttca aagacaaaag cctgctcttt gaccaactcc tcaccggact tggcagcata
      121 taaatcgatt tgctccaaac atgggccatc atactgctga tagccaaacg cctcggatgc
      181 cacgcgtagt ttttctatga gccactggcg ctgtgccatc tcctgtggat aaaaatcgcg
      241 ggtaccttta actttctgaa ttattttgct cattcataac ctcttattct tgctgtgcaa
      301 attataacac ccgatattaa agtatttgtg taatagttca aaatgccaaa agtgtaattt
      361 tagtgtaaaa ttcaattgca taataactga cgtgtaattc atgccgtcag cgaacaaata
      421 aggagcgtat actaatgaaa tacaaactac tcgctatcat aatcaccgtc atgcttgttt
      481 tgacggcatg tggtccaaaa gaaactgtta aacccactga ttctgtaagc gcttttcccg
      541 ttgtcgtgga tagttcccag gatacctatc cagccggaga cgcagtagac accgccacgg
      601 aagatactta tcccatcgcc gaaaccatcg cacaagacat ataccctgtt agcagtggcg
      661 aagcgatttc catcacagtc ttagccccca gctcaacgac gtctattccc atccttttag
      721 ccgcaaaata cctgcccgga atgacggttg aaatcttcga caaccatccc caggctcatg
      781 cccagtttat ggctgaagat aatctcgttt tggtgactgg tctttcagtc ggtgttgata
      841 tgttcaaaaa cgatgttccc ctgcaactca tcaacaccaa tgttactggc ctttcctatc
      901 tcgtaagctg caaccttgaa attaacagct ttgccgattt ggcgggtcat gaaatctacc
      961 tgccttttga aggttcaccc ctggaagaaa ccacccggtt ttttgttgaa caagctggtt
     1021 tgcagtacgg cgtagacgtc aaaccgattt acgcgccatt tgacacttcc accgcgctca
     1081 tcaaaaatgg cgatgatgct atcgtcgcca tgccccagcc cgccgcgaca gccatggctg
     1141 cccaggaatg tggtcactta ggtgccagtt tttacgacac ctggaacgcc atcaccggtg
     1201 ataccgatgg ctacccgcaa gttggcgcgt ttgccaaagc tgaatttgcc agcgcgcatc
     1261 ctgaatgggt gcaggctttc aaccaagctg tcgccaaagc catcgctgac ctccaggcta
     1321 accccgaagc cgcggtgaac gaagttgccg ataaattctc attccctgca cccgttttgc
     1381 ttcaggctct gcaggcgacc aggttcacag ttcaatctga ccagtcaatg aaagattccg
     1441 tcattcatta ctacgtaacg atcggaaaac cactggatga aagctacgag gcgttcttct
     1501 acgcccttcc ttaatgaatt aatcgggttt ggactgctca ttatcttatg ggcagtcctt
     1561 tccttgtttt ttcccgttta catcattccc tcaccaatcg aggtctttaa agccacacca
     1621 gaattcttaa acgcccagtt ttttagcgcg atgggtatca ccctgttgcg cattgttgtg
     1681 ggctttgcga tttcattttt catcggtact ggccttgcca ccctttttgc cggcacaaaa
     1741 ctctccgagc aactcagtgc catggtgcag gcttttcagg ttattcccgc ggtaacggta
     1801 ggcgttattt tggtcatcat ttttggtgtt ggcagtgcgc caccgattat tctcatcact
     1861 ttaatgacct tgcctttggt tttcatgaac accctgcaag ccttaaaatt cccagacccc
     1921 aaactgcttg aatatctgga ctccatcaaa gccgaccgca aattacgctt ttctgcgctg
     1981 atgaaacccc aaatcatcaa agttacccgc accaatctca ttttgggctt cagcatggcg
     2041 tccaaaattg ctgttaccgg agaatttatc ggctcgcagg acggactagg ttatctgctc
     2101 aacaaagccc gactcgtttt tgcgatgaaa gaagtctttt tctacatctt tttcttcatt
     2161 gtgcttactg ttttgtttca gggattagtg gaattgattt acgccacatc actaaggaaa
     2221 tacacctatg gcgactaaca cccaacaaaa cgttgttttg gaagccaatt ccattagcaa
     2281 gcagtttgat aacgaaatgg ttatcagcaa ctttagtttt aagttactac agaatgagag
     2341 acttcttctc ttcgcgccca gtggcgcggg caaaacaacc cttttgcgca tcttatgtgg
     2401 actgcttgaa gctgattcgg gctctttcag ctttcctggc ggcgctgaac cacacatcgt
     2461 tttccaggaa ccacgcctgt tcccccacat gacggttgac gaaaacataa agttgcccct
     2521 caaaagcccc catttcgcca gcaaacccaa cgccttggac ctgctgccag aatggtatca
     2581 gctaagtggc ttacacgaat accagggcca ctacccacat acgcttagcg gaggtatgaa
     2641 gcgcaaagta tctctcctgc gcgcgctcat caccctgcca gaacttctta tccttgatga
     2701 acctttccag tccctggata aaaaatcgcg tgaaattttt gtgcattcta tcttcaaaaa
     2761 catcccaaag ttgagcgttt tattcacttc acacgatatc ttagacgtcg agataactgc
     2821 cacgacgatt ttattaataa agtcccctaa attacagaat ccgattataa ta
//
LOCUS       DAOSFC010000184         2862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_33298_length_2862_cov_6.390452, whole genome shotgun sequence.
ACCESSION   DAOSFC010000184 DAOSFC010000000
VERSION     DAOSFC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33298_length_2862_cov_6.390452"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..642
                     /gene="msrA"
                     /locus_tag="PK791_05200"
     CDS             130..642
                     /gene="msrA"
                     /locus_tag="PK791_05200"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOH92745.1"
                     /translation="MQSKQTDTIVLAGGCFWGMEELFRQEIGVLDTECGYTGGENENP
                     SYQNHPGHAEALRIVFDTTQTSLLKLLDFFFRMHDPTTLNRQGYDIGTSYRSAIFYKD
                     ETQKQCAEKMIELVNTSGMWRNLVATTLEPLGTFWPAEDYHQDYLQKNPGGYTCHYIR
                     KTIPTYLKAD"
     gene            819..>2862
                     /locus_tag="PK791_05205"
     CDS             819..>2862
                     /locus_tag="PK791_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOH92746.1"
                     /translation="MAVINKFTQKAKRVLSLAQLEAERYKTQKISNEHLLLGLMIEKG
                     TIANRVLQDLGVSEEAIRKVVEEEHKPDDEIKSYSLAEETKNLLERALEEAKLGGASI
                     VGTEHLLLAMATDEKSTGMQVLLSLGVTPEQIRRQTKRVLKSAKEELEENVDTAPPKK
                     DTDAPAMAGAPRLPRRTRVEKDDSKTPLVDQLATDLTALAEADKLDPVIGREKEIERV
                     IQILARRTKNNPALIGEPGVGKTAIVEGLAQRILTGDVPALLLDKRVLQLDVGSLVAG
                     TMYRGQFEERLKRVIDELKTSGAIVFIDELHMLVGAGSAGSQVDAANILKPALSRGEL
                     QVIGATTLNEYRKSIESDAALERRFQPIVVEQPSVDETIEILYGLREKYEEHHRLHIS
                     DEAIETAAKLSSRYVSERFLPDKAIDLVDEAASRVRMYKSDTAVNSKDLMSKLRKVRA
                     ELKEAQAVEDNDNILALLKRQEELEASLKSLQTGWDRANAPTVTGEDVAEVISMWTGI
                     PLTQLAQEESERLLNMEEALSESIIGQEEAITAIAKAVRRARAGLKNPRRPVGSFMFL
                     GPTGVGKTELTKALARLLFGNEEALIQIDMSEFMERHSMSRLVGAPPGYVGYDEAGQL
                     TEAIRRRPYSIVVFDEVEKAHPEALNMLLQIMEEGHLTDAKGLKVDFKNAIIIMTTN"
BASE COUNT          798 a          691 c          731 g          642 t
ORIGIN      
        1 tggtaagtct ttgaaaaaac agctgttggt ttagacaaag caataaaaaa tcaggataaa
       61 tttgatagaa tttctcacgg cataatgttg tgccgtgaga atagtttaag aacaaggaca
      121 atgcgcgcga tgcaatcaaa acaaacggac acaattgttc tcgccggagg atgcttttgg
      181 ggtatggaag agctattcag acaagagatt ggcgttttgg acaccgaatg tggctacacc
      241 ggtggcgaaa acgaaaatcc cagctatcaa aatcaccctg ggcatgctga agcactgaga
      301 atcgtctttg atactaccca aaccagtctg ttaaaactcc tggatttctt tttccgtatg
      361 cacgatccca cgacgctaaa ccgccagggc tacgacatcg gcacatccta ccgcagcgcg
      421 atattttaca aggatgaaac gcaaaaacaa tgcgcggaga aaatgattga gcttgtcaat
      481 acaagtggca tgtggcgcaa tctggtggcg accacactcg aacccctggg tactttttgg
      541 ccggctgagg actatcatca ggactatctg caaaagaatc ccggtggcta cacctgtcat
      601 tatatacgca agactatccc gacttatctc aaggcggact aatcttttca gactattaac
      661 agttcatcaa aaaaagatgc ttgcgtttgt gcaaactctt tgctttggta caatcaatac
      721 aggttgggtt gcttacatga ttagcttggt tttcacttta aaggaaagcc tttccacgat
      781 ggcgcaacca acactaatat tcttacagga ggactacctt ggctgttata aataaattta
      841 cacaaaaagc aaaaagggtg ctcagcctgg cacaattgga agctgaacga tacaaaaccc
      901 aaaagatttc aaatgaacat cttttgttgg gtttgatgat tgaaaaaggc accatcgcca
      961 accgcgtttt acaggatctt ggcgtgagtg aagaggcaat tcgcaaagtc gttgaagaag
     1021 aacacaaacc tgatgacgag ataaagtctt acagtctcgc cgaagagacc aaaaacctgc
     1081 ttgaacgcgc gctggaagaa gcaaaattgg gcggtgcgtc aatcgttggc accgaacatc
     1141 tcttacttgc catggcaacg gacgagaaaa gtacaggcat gcaggtgctt ttgagtttag
     1201 gtgtgacacc cgagcaaatc agacgtcaga ccaaacgcgt ccttaagagc gcgaaagaag
     1261 aactcgaaga aaacgttgac accgctcctc ctaaaaagga tactgatgcc cccgccatgg
     1321 caggcgcgcc acgtttgccc aggcgcacac gcgtcgagaa agacgacagc aagacgcccc
     1381 tggtggacca gttagccacc gatctgactg cgcttgccga agcggataaa cttgacccgg
     1441 tcattgggcg cgagaaagaa attgagcgtg tcattcaaat tttggcgcgc cgtaccaaaa
     1501 ataacccagc cctgattggc gagccgggcg tcggcaaaac cgctatcgtt gaaggacttg
     1561 cccagcgcat tctcaccggc gatgttcccg cattgttgct cgacaagcgc gtgttgcaac
     1621 tggacgtggg ctctttggtg gcgggcacaa tgtaccgtgg ccagtttgaa gaacgcctga
     1681 aacgtgtgat tgacgagctc aagaccagtg gcgcaatcgt ctttattgac gaattgcaca
     1741 tgctggttgg agctggttca gcgggttcgc aggttgatgc ggccaacatt cttaaaccgg
     1801 ctctttcgcg gggtgagttg caagtcattg gcgcgaccac tctcaatgaa tatcgcaaga
     1861 gtatcgagtc tgatgccgcg ctggagcgtc gcttccaacc gattgtggtt gagcagccca
     1921 gtgtggatga aactattgaa attctttacg gcttgcgcga gaaatatgaa gaacaccatc
     1981 gtctgcatat cagcgatgaa gccattgaaa ccgccgcgaa gctgagttcg cgctatgtca
     2041 gtgagcgttt cttgcccgac aaagccattg acctggtgga cgaagccgct tcgcgtgtgc
     2101 gcatgtacaa aagcgacacg gctgtcaaca gcaaggattt gatgagtaaa ctgcgtaaag
     2161 tccgtgctga gctcaaggaa gcccaggcgg ttgaagacaa cgacaacatt ctggcgctgc
     2221 taaaacgtca ggaagaactt gaagccagcc taaaaagctt gcaaactggt tgggatcgcg
     2281 ccaacgcgcc taccgttact ggcgaagatg tggcagaagt catctcgatg tggacgggca
     2341 ttccccttac acaactcgct caggaagaaa gcgaacgcct tttgaacatg gaagaagctt
     2401 tgtccgagtc gattatcggt caggaagaag ccattactgc catcgccaag gctgtgcgac
     2461 gcgcgcgggc tgggcttaaa aacccacgcc ggccagttgg ctcttttatg ttcctgggtc
     2521 ctaccggtgt tggcaagacc gaactcacca aagcgctggc acgtttgctc tttggcaacg
     2581 aagaagcgct cattcaaatt gacatgagcg agtttatgga gcgtcacagt atgagccgtt
     2641 tggttggcgc acctccagga tatgtcggtt acgatgaagc cggtcagcta accgaagcta
     2701 tccgccgccg accctactcg attgttgtct ttgacgaggt cgaaaaagcc catccggaag
     2761 ccttgaatat gcttttgcaa atcatggaag aaggccacct taccgatgcc aaaggtctga
     2821 aagtggactt caaaaatgcc atcatcatta tgaccaccaa cg
//
LOCUS       DAOSFC010000185         2851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_33453_length_2851_cov_5.094421, whole genome shotgun sequence.
ACCESSION   DAOSFC010000185 DAOSFC010000000
VERSION     DAOSFC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33453_length_2851_cov_5.094421"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /locus_tag="PK791_05210"
     CDS             complement(<1..2486)
                     /locus_tag="PK791_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92747.1"
                     /translation="MSSLTRVVLSQLTPPAQRGNVLARERLDDALKQSLHYPLSIVCA
                     GTGYGKTTSILGFVSKLDIPVFWYSISPAERDPKLFLANFFTAFNRGEYKFGQTALRL
                     LLDSDAEGREAIIALVNALSEGLVSKALLVLDDFQSVEESDEIMKLIDWFINHLPANL
                     HVLIASRIPLSFPSMSRWQAQGSLFEIGRDTLSFSVGEVEDLFHKVYQVKLEPQDVRK
                     LHNRTEGWAVALQAVWHNLRSYPEGQLSSLLEDEKPISASYLFDYLAEEVFDKQEPQR
                     QVFLAYCSILKFLESDTCDFLLDRTDTQDELHELFSEGLFLEQLQPGVYRFHHIFKNF
                     LREQLHRKPGLAKDLHRKIASYYIAHEDWQEAISHLLLAEDYPRVMQLLDDVGNSLLS
                     RGLHQSVGYFVGQLPEYLLNDYAYGNYLLGEVQRNDALFEQALESYRAAQRIYQKQDN
                     SYGMALALRGQAQVYLDTLRPSNASQLLNRALKLMDAQESPHEVAGLLTQIAENQLNQ
                     GEPLKAAESLAKARDLSFDYQEEQDYIEARTMLRTGRLQDGLALLNRLEQNNVALALP
                     KPQRFHRDTSLLTSLFHVILGNQEKALAAAQKGLNAAEKSHASLGIRIGKMRLAHAKQ
                     LDIDRTLQPDHVEEIKQLYEDAMRDMAVARINVEPLWGMCRLLGYAGELGEAMRVAQA
                     AIALATQSGDVWIGMMVRISLGASLAQAGEFESASNELALAETMALQVGDEFALMACW
                     IWQAYLAQLQGYKNSCRLFLEQALAVISDKSYEFFLIKPSLLGPNDIACFVPLLIEAR
                     KAGIEPALVERLLREQGFEKINYHPGYRLRL"
     gene            2585..>2851
                     /locus_tag="PK791_05215"
     CDS             2585..>2851
                     /locus_tag="PK791_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOH92748.1"
                     /translation="MQELIAGIHAHTTFSDGAGTHAELAQAAMKAGLDVLITTDHNVL
                     VQGMDGYLQKGRRKMLLLAAEEIHDPTRQPGGNHMLALGANRELA"
BASE COUNT          757 a          769 c          619 g          706 t
ORIGIN      
        1 agccgcaagc gatagcctgg atgataattt attttttcaa aaccttgttc tcttagtaat
       61 ctctccacca gtgccggttc aatgcccgct tttcgggctt caatcagcaa gggcacaaag
      121 caagctatgt cattcggccc aagcaaactg ggcttgatga gaaagaattc atagctctta
      181 tcactaatca ctgccagagc ttgttccaaa aacaaacggc atgaattttt ataaccctgc
      241 aactgtgcca gataggcttg ccaaatccag caagccatta aggcaaactc atcgccaacc
      301 tgtaaggcca tcgtttcggc aagtgccagc tcgttggaag ctgattcaaa ttccccggct
      361 tgtgccaggc tcgcgcccaa agaaatgcgc accatcatcc caatccacac atcgccggat
      421 tgcgttgcca gagcaatcgc ggcctgggca acgcgcatcg cttcgcccaa ctcacccgca
      481 taacctagca acctgcacat tccccaaagc ggttcaacat tgatgcgcgc cacagccata
      541 tcgcgcatcg cgtcttcata aagctgcttt atctcttcta catgatcagg ctgcaaagtt
      601 ctgtcaatat ccagttgttt agcgtgtgcc agacgcattt tgccaatgcg aatgcccaac
      661 gatgcatggc ttttttcggc cgcgtttagt cctttttgtg ctgccgctag ggctttttcc
      721 tgattaccga gaatgacatg gaataaagat gtcaataagg atgtatcgcg gtggaaacgc
      781 tggggttttg gcaaagctaa agccacgttg ttttgttcaa gtcgattaag caaagccagc
      841 ccatcttgaa gtctgccagt cctaagcatc gtacgcgctt caatataatc ttgttcttcc
      901 tgataatcaa aacttaaatc gcgggccttg gccagacttt cagcagcctt cagcggctcg
      961 ccctggttca attgattttc agcgatttgc gtcagcaaac cagccacctc atggggactt
     1021 tcctgggcat ccatcaactt caacgcccgg ttaagcaact gactggcgtt actgggacgc
     1081 aaggtgtcta aataaacctg agcctgacca cgcaatgcca gtgccatgcc ataactgttg
     1141 tcctgttttt ggtaaattct ttgcgccgcc cgataagatt ccaatgcctg ttcaaacagg
     1201 gcatcgttgc gctgaacttc acccaaaaga taattgccgt atgcgtaatc atttaacaaa
     1261 tactcaggca gttgacccac aaaataaccc acactctgat gcaaaccccg gctcaacaga
     1321 gagttgccaa cgtcatccaa aagttgcatc acccgcggat aatcttcagc caacagcaag
     1381 tgtgaaatcg cttcctgcca gtcttcatgg gcgatatagt agctggcaat ttttcgatgc
     1441 agatctttag ccaatccggg ttttctatgt aactgttcac gtaaaaagtt tttaaagata
     1501 tgatgaaaac gatacacccc gggttgaagt tgttccagga acaagccttc actaaaaagt
     1561 tcgtggagtt cgtcctgggt atcggtcctg tccagcagga aatcgcaggt atcgctttcc
     1621 aaaaacttca ggattgaaca ataggccaga aaaacttgtc gttgcggttc ttgtttatca
     1681 aaaacttctt cagccaggta atcaaaaaga tagctcgcgg aaataggttt ttcatcttcc
     1741 aacaatgaac tcagttgccc ttcaggatac gaacgcagat tatgccaaac agcttgcaag
     1801 gcaaccgccc agccttcggt tcggttatgt aatttgcgca catcctgtgg ctccagcttg
     1861 acttggtaga ctttgtggaa caagtcctca acctcaccca cagaaaaact taaagtatcc
     1921 cgcccgattt caaacaaact tccctgggct tgccagcggc tcatcgatgg aaaactcagc
     1981 ggtatgcgac tggcaatcaa aacatgcaaa ttagctggca aatgattgat gaaccagtca
     2041 atcagcttca tgatttcatc ggactcttcc acactctgaa aatcgtccag caccagcaaa
     2101 gcctttgaca ccagcccttc gctcaaagca tttaccaagg cgatgatggc ttcccgccct
     2161 tcagcatctg aatccaacaa aagacgcagg gcggtctggc caaatttgta ctcaccgcga
     2221 ttaaaagcgg taaagaaatt cgccaggaaa agcttggggt cgcgctcggc gggcgagatg
     2281 gaataccaaa acaccggtat atccagcttt gagacaaaac ccaggatgga tgtcgtcttg
     2341 ccatagcccg tccccgcgca cacaatgcta agcggataat gcaaagattg ttttagtgcg
     2401 tcgtccagtc gttcacgcgc taaaacattg cccctttggg caggtggtgt aagctgacta
     2461 agaacgaccc gagttaatga actcatgctc taattttatc atcagtttgt gcttaattct
     2521 tttactaaat tatattttag gtcacttttt cgacctaaaa gtctttggcg tgtataattc
     2581 ccttatgcaa gaactcatcg ctggcattca cgcccacacg accttttcag acggcgcggg
     2641 gacacacgcc gagttagccc aggccgcaat gaaagccggc ctggacgtgc tcatcaccac
     2701 cgaccacaac gtgctggtgc agggcatgga tggctatctg caaaaaggac ggcgtaaaat
     2761 gctcctgctt gccgctgaag aaatccacga ccccacccgc cagcccggtg gcaaccatat
     2821 gctcgcgctt ggtgccaacc gtgagctcgc c
//
LOCUS       DAOSFC010000186         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_33480_length_2849_cov_6.270938, whole genome shotgun sequence.
ACCESSION   DAOSFC010000186 DAOSFC010000000
VERSION     DAOSFC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33480_length_2849_cov_6.270938"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PK791_05220"
     CDS             complement(<1..1122)
                     /locus_tag="PK791_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH92749.1"
                     /translation="MEKTTISAETKKSKFPYWLQLALIFFLGWVFMYATRNILSASMV
                     EIGDEFKLTGVQLGLLNTLFYLAYTLSQIPSGIIADKIGLKVVLVFGFVFYGIFTGLT
                     GIAASFTILLIMRILVGIGQGTYYGPQYALSSLNVPKKNRGVGNAIINSGMAFGTSLG
                     LIGGGFITSKLGWRYNFYIFAIPVFLIALLFQFVIRKNKASEQERPADHFENAEEAHA
                     EHKGDRRNIIAIFIIVFCSFFGFFFIQSWLPYYLQVERGIAPENTGFVSSLVAWSSIP
                     GALLWAHLSDRLKTRKPFMIGLLFFAVISTLGAVLLENFPLLIVSLILYGLTGKLGLE
                     PILVSAIADNAPSTNPGTLFSTFNSIGVSASILAPIIGGF"
     gene            complement(1232..2098)
                     /locus_tag="PK791_05225"
     CDS             complement(1232..2098)
                     /locus_tag="PK791_05225"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HOH92750.1"
                     /translation="MTQELKSGLISSGLVQIPFSKASGQIDSLFDHSFNIAFEEDLLN
                     VASSRLDCSSFGISLPELDFENLRMYVLIGDRAIRKEDRLTIYTSLGGVFEIKLSRLT
                     NVDLKIIPVQADATLIRTIIKTLDEFSIEENTGLVIDAYTQKYLDALTAPFLSQDEFE
                     ATVNFLIGRGKGLTPSGDDILLGYLMTFMLFDLPISSELLYSKEVLKKTTLVSANYLK
                     MLGLGYIGESFKRFNKAIQYRKIQHLRLSLSRITEIGASSGYDTLMGVKLALKRILAE
                     NVFSEKSINSAL"
     gene            complement(2098..>2849)
                     /locus_tag="PK791_05230"
     CDS             complement(2098..>2849)
                     /locus_tag="PK791_05230"
                     /inference="COORDINATES: protein motif:HMM:NF018275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOH92751.1"
                     /translation="NVIVSKAIAMGDEFHQRNIAGSLLILKELVPLITTLDMDNNEKQ
                     RVIKFLADTDQFFLNVMMATSKAIVDYARKIQEGTVVTTMTRNGKNFGIRIAGMGDEW
                     FTAPVNKPRGLYFTGYTEEDGNPDIGDSAITETVGVGAIVTVAAPAVTRFVGSGGGYD
                     DALAITDRQNEIFITNNPNWPIPNWNFKGAPCGIDARKVVATGIAPLINTGIAHRIAG
                     LGQIGAGTAEAPVACFEKAIVAYAKKLGIEA"
BASE COUNT          857 a          643 c          621 g          728 t
ORIGIN      
        1 gaaaccgccg ataataggcg caaggattga ggcactcaca ccaatagaat tgaaggtact
       61 aaacaatgta cctgggttcg ttgatggcgc gttatcagca atagcagaaa cgagaatagg
      121 ctccagccct aatttccctg tcagaccgta taggatcaat gacacaatca gcaaggggaa
      181 gttttccaaa agaaccgcgc ctaaggtgga tatcaccgca aaaaagagca ggccaatcat
      241 aaaaggcttg cgggttttaa ggcggtcaga aagatgcgcc cacagcaacg cgccggggat
      301 ggatgaccac gcaaccaaag aactgacgaa gcccgtattt tccggtgcga taccgcgttc
      361 aacttgaaga tagtaaggca accagctttg gataaagaaa aagccaaaga aggaacaaaa
      421 gacgatgatg aaaatcgcaa taatattacg tcgatcgcct ttgtgctcag catgggcttc
      481 ttctgcattt tcaaagtgat cagcgggtcg ttcctgctcg ctggctttgt tcttcctaat
      541 cacaaactgg aagagaaggg caatcaaaaa cactgggata gcaaaaatgt aaaagttata
      601 gcgccagccc aatttgctgg tgataaaacc accaccaatc aaacccaggg aagtaccaaa
      661 tgccataccg ctgttgataa tcgcattacc aacaccacgg tttttcttgg ggacattcaa
      721 tgacgaaagc gcatactgtg gtccataata cgttccctgg ccgattccaa ccaaaattcg
      781 catgattaga aggattgtaa atgaggcagc aatgcccgtt agtccagtga agataccata
      841 aaagacgaag ccaaagacta gaacgacttt aagaccaatt ttgtcagcaa taataccaga
      901 ggggatttgc gacaatgtat atgctaaata aaacaaggtg tttaaaagac ccagctgaac
      961 accggttaac ttgaactcat cgccaatttc gaccatcgac gcgcttaaaa tgttgcgcgt
     1021 ggcgtacatg aacacccaac caaggaagaa gatcaaggct aattgaagcc aatatgggaa
     1081 cttggatttc ttggtttcag cgcttattgt ggttttttcc atttttcaat ctccttatac
     1141 tctttcgtat agtgaagcta atcattaaca ttaacaacta tgttttgaaa aggttatgca
     1201 aatttaacca taggctactc cagttattcc tttataaagc tgaattgatt gatttttcgc
     1261 taaaaacgtt ctcagcgagt attctcttaa gggccaattt tacacccatt aaggtgtcat
     1321 agccggaact tgcaccaatt tcggtaattc tcgataaact aagtcgcaaa tgttgaattt
     1381 tgcgatattg aatcgcctta ttaaagcgct taaaactttc accgatatag cccagaccaa
     1441 gcattttcag atagttggcg ctaaccaaag tcgttttttt aagcacttcc ttcgaataaa
     1501 gcaattcact ggaaatgggc aaatcaaaca acataaaagt catcaaatat cccaggagaa
     1561 tgtcatctcc actgggtgtc aatccctttc cgcgcccgat gagaaaatta actgttgctt
     1621 caaactcatc ctgggacaaa aaaggagccg tgagtgcgtc caaatacttt tgggtatagg
     1681 catcaataac taagccagta ttctcttcaa tagaaaactc atccaaagtc ttgatgattg
     1741 ttctaatcaa agttgcatct gcttgcacag gaataatctt taggtcaaca ttcgtcagcc
     1801 ttgaaagctt aatctcaaac acgccgccga gactggtgta aatcgttaag cggtcttcct
     1861 ttcggattgc acgatcgcca atcaggacat acatgcgaag attttcaaaa tcaagttcgg
     1921 gcaagcttat tccaaagctt gaacagtcca aacgtgaact agccacattg agcaggtctt
     1981 cctcaaaagc aatattaaag ctgtggtcaa acagactatc tatctgacca ctagcttttg
     2041 agaaagggat ctggactaat cccgaactga ttagtccaga tttcaactct tgagtcatta
     2101 agcttcgatg cctaatttct tggcgtaagc cacaatggct ttttcaaaac aggccacagg
     2161 cgcttcagca gtaccagcac caatttggcc aagaccagcg atgcggtgag caataccggt
     2221 gttgattaag ggtgcgatac cagtagcgac aacctttcgg gcgtcgatgc cacaaggcgc
     2281 gcccttgaag ttccagttgg gaataggcca gttgggattg ttggttatga agatttcatt
     2341 ttggcggtcg gtaattgcca aagcgtcatc ataaccgcca ccagaaccga caaagcgtgt
     2401 tactgcaggt gcagcgactg taacaatcgc gccaacgcca actgtttcgg tgatggctga
     2461 gtcgccgatg tcggggttgc cgtcttcttc cgtgtaaccc gtgaaataaa gtcctctggg
     2521 tttgttgaca ggtgcggtaa accattcgtc gcccattcca gcgatgcgga taccaaaatt
     2581 cttaccgttg cgggtcatcg tggtaaccac agtaccttct tgaatcttgc gcgcgtagtc
     2641 aacaatcgct ttactggttg ccatcatgac gttgaggaag aactggtcgg tatcagccag
     2701 gaacttgata acacgttgtt tttcgttatt atccatatcc agcgtggtga taagcggaac
     2761 caattctttt aggatgagca atgagccggc aatgttgcgc tgatggaatt catcacccat
     2821 ggcgatagct ttcgagacga tcacattaa
//
LOCUS       DAOSFC010000187         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_33644_length_2841_cov_4.304738, whole genome shotgun sequence.
ACCESSION   DAOSFC010000187 DAOSFC010000000
VERSION     DAOSFC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33644_length_2841_cov_4.304738"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PK791_05235"
     CDS             <1..407
                     /locus_tag="PK791_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH92752.1"
                     /translation="AELEQLRLRVAKVAFASQMDNLQTIMDVPVLTATIPNVSADALR
                     ELADEFRQKHESGVVVLATTINDKPSFIAALTPDLVKRGLKAGDLVKRVAKVVGGGGG
                     GRPDMAQAGGKDLSKIAEALAQVAPYIKENLK"
     gene            411..>2841
                     /gene="mutS"
                     /locus_tag="PK791_05240"
     CDS             411..>2841
                     /gene="mutS"
                     /locus_tag="PK791_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOH92753.1"
                     /translation="MAKQVTPLRQQYLDIKKNYPNAIVFFRLGDFYETFDEDAEKTAK
                     ALDIVLTSRPVAKGVRVPMAGIPYHAVDNYIGRLIDKGYHVAICEQVGEQPEKGLFER
                     DVVRVITPGTLVEPNLLQSEKNNYLACIVTYENHAGLAYVDISTGEFATTQIQNDDIL
                     VTLRAELERLAVAELLVAEDWQVPPGFPDNITTIPQWYFEPGRAKEAIKARFNVSSLD
                     GLGIRDLPWAMRASGALIKYLQDTQKGMDSTQLSFSTYSLSDFMVLDAETRRNLELTE
                     TLREQKLEGSLLGVLDKTQTSGGKRMLRSWVNKPLLSLDAINKRLDLVEFFFNNGVLR
                     QDLNAILTKLHDLERLVNRINSGTARPPDLVALREDFQGLPHIIALLKDKHPVLDEIL
                     AKVSPFDQERERLEAAIEDEPPATLANIGIIRPGFSEALDKTLYASKHAREWINNLES
                     YERQRTGIKNLRVGYNKVFGYYIEVSRGQTDQAPDDYIRKQTLVNSERYITPELKEYE
                     SLVLNAEAQIHDVELKVWKELCDEISSVSTQILKTAQAIAHIDCCVALASVAALNNYT
                     RPTLREEKGIRIIEGRHPVVERMRSVDRYIPNDLVFEEGEIIKLITGPNMSGKSTILR
                     QLVLIVLMAQIGSFVPAKEAEIGLVDRVFTRIGAQDAIHQGQSTFMVEMIETANILNN
                     ATPRSLLILDEIGRGTSTYDGISIAWAIIEYLHSHPKLKPYTLFATHYHELTDLAELF
                     PGVRNYNVAVTEADGKVIFLHKIVPGSADRSYGIHVAQLAGLPAPVITRANQVLKQLE
                     ESGERALPKQIA"
BASE COUNT          731 a          795 c          689 g          626 t
ORIGIN      
        1 aggctgaact tgaacagctg cgattgcgcg tcgcgaaagt tgcctttgcc agccaaatgg
       61 acaacttgca aacaattatg gatgtgccag tcctcaccgc gacaattccc aacgtcagcg
      121 ctgacgcttt gcgcgaactc gccgatgagt tccgtcaaaa gcatgaaagt ggcgtggttg
      181 ttttggcgac gactatcaac gacaagcctt ctttcatcgc cgcattaacc cccgaccttg
      241 ttaaacgcgg attaaaagct ggggatcttg tcaaacgggt tgccaaggtc gttggtggcg
      301 gtggcggtgg gcgtcctgat atggcccagg ccggcggcaa ggacctctcc aaaatcgccg
      361 aagctcttgc ccaggttgcc ccttacataa aggaaaatct aaaatagttt atggcaaaac
      421 aagtcacccc actgcgtcag caatacctgg atattaagaa aaactatcca aacgcgatag
      481 tcttctttcg cttgggtgac ttttatgaaa ctttcgacga agacgccgaa aaaactgcta
      541 aagccctgga tatcgttctc acctcgcgtc cggttgccaa aggcgtgcgc gtgcctatgg
      601 cgggtatccc ctaccacgcg gttgacaatt atatcgggcg tttaatcgac aaaggctatc
      661 acgttgccat ctgcgaacaa gtcggcgaac aacccgaaaa aggcttgttt gagcgtgacg
      721 tggtacgtgt gatcaccccc ggcacactgg ttgagcctaa tctcctgcaa agcgagaaaa
      781 acaattacct ggcatgcatc gtcacctacg aaaaccatgc cggtctagcc tatgtggata
      841 tttcaaccgg tgaatttgcc acaacccaaa ttcaaaatga tgacattctc gtcaccctgc
      901 gcgctgaact tgaacgcctt gcggtggcgg agttgctggt tgcggaagac tggcaagtgc
      961 cacctggttt tccagacaac attactacca ttccacagtg gtatttcgaa ccgggcagag
     1021 ccaaggaagc cattaaggcg cgctttaatg tttccagtct tgatgggttg ggcattcgcg
     1081 atttaccctg ggcgatgcga gcctctggcg cgcttatcaa atatctgcaa gacacgcaaa
     1141 aaggcatgga ctccacgcaa ctcagtttca gcacctatag cttgagcgat ttcatggtgc
     1201 tggacgccga aacccgtcgc aacctggaac tgaccgaaac cctccgcgaa caaaagcttg
     1261 aaggttcgct gctgggcgtg ctggataaaa cccaaacctc aggtggcaag cgcatgctgc
     1321 ggtcgtgggt caacaaaccc ctgttgagcc tcgacgcgat taacaagcgg cttgatttgg
     1381 tcgagttttt cttcaataac ggtgttttgc gccaggattt aaacgccatc ctgacaaaac
     1441 tgcacgattt ggaacgtttg gtcaaccgca tcaactccgg cactgcccgc ccacccgatt
     1501 tggtcgcctt gcgggaagat tttcagggct tgccacatat cattgcctta cttaaggaca
     1561 aacacccggt cctggatgag attttggcaa aggtcagccc ctttgaccag gaacgcgaac
     1621 gcctggaagc cgccatagaa gatgaaccac ccgcgactct ggccaacatc ggcatcattc
     1681 gccctggctt ttcggaagcg ctggataaaa ctctctacgc gtccaaacac gcccgtgaat
     1741 ggataaacaa ccttgaaagc tacgaacgcc aacgcaccgg catcaaaaac ctgcgcgtgg
     1801 gctacaacaa agtcttcggc tattacattg aagtctcccg cggtcaaacc gaccaggcac
     1861 ccgatgatta catccgcaag caaaccctgg tcaacagcga acgctacatc acccccgaac
     1921 taaaagagta cgaatcactc gtcctcaacg ccgaagcaca aatccatgac gtcgaactca
     1981 aggtttggaa agagctttgc gatgagattt cttccgtttc gacacaaatt ctcaaaaccg
     2041 cccaggccat cgcgcacatt gactgttgcg ttgctttggc aagtgtcgcg gcattgaaca
     2101 actacacccg ccccactctg cgcgaagaaa aaggcattcg catcatcgaa ggtcgccacc
     2161 ctgttgtcga acgcatgcgt tcggtggatc gttacattcc caacgacctt gtttttgaag
     2221 aaggcgagat tatcaaactg attaccggcc ccaacatgag tggtaaaagc acgattttac
     2281 gccagctggt tttaatcgtg ctgatggcac agattggcag ttttgtgccc gccaaagaag
     2341 ctgaaatcgg cctggtagac cgcgttttca cccgcatcgg tgcgcaagac gccattcacc
     2401 agggacaaag tacctttatg gtcgagatga ttgaaaccgc caacattctc aacaatgcca
     2461 cgccccgcag tttgctgatt ttggacgaaa tcggacgcgg aaccagtacc tatgatggca
     2521 tctcaatcgc ctgggcaatt attgagtatc tgcacagtca ccctaaacta aaaccttaca
     2581 cgctttttgc cacccactac cacgaactga ccgatttggc agaactgttc cctggcgtgc
     2641 gcaattacaa cgtggcggta accgaagccg atggcaaggt cattttcctg cacaagattg
     2701 tgcctggcag cgcggaccgg tcctacggta ttcatgtggc gcaactggcc ggactgcccg
     2761 caccggtcat cacccgcgct aatcaggtct taaaacaact ggaggaaagt ggcgaacgcg
     2821 ccctgcctaa acagatagcc g
//
LOCUS       DAOSFC010000188         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_33887_length_2827_cov_3.969336, whole genome shotgun sequence.
ACCESSION   DAOSFC010000188 DAOSFC010000000
VERSION     DAOSFC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33887_length_2827_cov_3.969336"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PK791_05245"
     CDS             <1..484
                     /locus_tag="PK791_05245"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOH92754.1"
                     /translation="TSKLAGTERGLGDKPQATFSTCFGAPFLPLKPSIYAELLSKRLE
                     KHGTKVWLLNTGWAGGGFGVGERIALPLTRAMIDAVLSGSLDDVETHTHPIFNIEIPN
                     AVEGVPDEVLNPEKSWTDFDAYKIEANGLAEKFIKNFAQYEDSVSEKVTLSGPKILKD
                     "
     gene            512..1804
                     /gene="murA"
                     /locus_tag="PK791_05250"
     CDS             512..1804
                     /gene="murA"
                     /locus_tag="PK791_05250"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOH92755.1"
                     /translation="MDRFVINGGIPLSGVMIPQGNKNAALPLMSATLLSEEPVILHNM
                     PEIRDVQTMRQLLQSLGVSIRQIGNSSSWEFHAKNIRPADLDPELCRKIRASILLAGP
                     MTARYGALELPPPGGDVIGRRRVDTHILALEKLGASFDIDRGMFKFSANKLQGTEILL
                     DEASVTATENTIMAASLAKGQTIIRNAASEPHIQELCHLLNNMGANIENIGSNTLLIQ
                     GVDKLHGGEFTIGPDYLEVISFVGAAVVTRGSIHIKNAGVEHLGMIRLVLKRLGVDMR
                     FEGDDLIVDANQRLEIEPDLGNAIPEISVMPWPSFPTDLMSIAIVIATQSKGTVLFHD
                     WMYPSRMFFTDKLVSMGAQIVLCDPHRCIVNGPTPLTGETLDSPDIRAGTALVLAALS
                     AEGTSVIRNVAQIDRGYSQIEVKLRAIGADIQRISDND"
     gene            complement(1885..2232)
                     /gene="trxA"
                     /locus_tag="PK791_05255"
     CDS             complement(1885..2232)
                     /gene="trxA"
                     /locus_tag="PK791_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH92756.1"
                     /translation="MTNEPINVTDAAFEKTVLQNELPVIVDFWAPWCGPCKMLRPILD
                     KMAKEFAGKLIIAKVNTDEHQEWASKYGVQGIPTMLMMKDGKIVHRQVGVLPEPMLRD
                     TIKNFLEVATGKA"
     gene            complement(2308..2706)
                     /locus_tag="PK791_05260"
     CDS             complement(2308..2706)
                     /locus_tag="PK791_05260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOH92757.1"
                     /translation="MTLNKTEKEVQNYIKGRNKSFKNAFEGLLYAIRTEKNVMIHAII
                     TVLVISVSFIFKLSQMEWIAVLIAIALVWIAELINTALETIVDIICPEIHPLAKKAKD
                     LSAAFVLIAAILAFCIGCIIFIPKLLSFIR"
     gene            2757..2827
                     /locus_tag="PK791_05265"
     tRNA            2757..2827
                     /locus_tag="PK791_05265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2789..2791,aa:Gly,seq:ccc)
BASE COUNT          827 a          592 c          578 g          830 t
ORIGIN      
        1 tacttctaaa ctcgctggta ctgaacgtgg actgggtgac aaaccccagg caacattctc
       61 aacatgtttc ggtgcgccat tcttgccact taaaccttca atatatgctg aattattgtc
      121 caaacggctc gaaaagcatg gcacaaaagt ttggcttttg aataccggtt gggctggtgg
      181 tggctttggt gttggtgaac gtattgcctt gccattgacc cgtgcgatga ttgacgccgt
      241 tttaagtgga tcactggatg atgttgaaac ccatacacac cccatcttta acattgagat
      301 tccaaatgct gttgaaggtg taccggatga agttttgaat cctgaaaaat cctggacaga
      361 ctttgatgct tataaaattg aagcgaatgg cttggcagaa aaatttatta agaactttgc
      421 ccagtatgaa gattcagtat cagaaaaagt aacactttct ggtcctaaaa ttttgaaaga
      481 ttaattgaaa atatttagaa aaggacaaaa catggacaga ttcgtaatta atggtggtat
      541 tccactttct ggcgtgatga ttccgcaagg caataaaaat gcggctctgc ccttaatgtc
      601 tgcgacgctt ctaagcgaag aaccggtcat tctgcacaat atgcctgaaa ttcgcgatgt
      661 tcaaacgatg cgacaacttc tacagagtct tggtgttagt attcggcaaa ttggtaattc
      721 aagttcctgg gagtttcatg ctaagaacat tcgaccagca gacctagacc cggaactatg
      781 ccgtaaaata cgtgcgtcta tcttattagc tggaccaatg accgcccgct atggtgcgct
      841 ggaactacca ccacctggtg gtgatgttat tgggcggcgt cgtgttgata cacatatatt
      901 ggcacttgaa aaacttggcg cttctttcga tatcgaccgg ggtatgttca agtttagtgc
      961 taataaatta caaggtaccg aaattctttt ggatgaagcc agtgtaaccg caactgaaaa
     1021 caccatcatg gcagcttccc tggcaaaagg tcaaactatc attcgcaatg cggcatccga
     1081 accccacatt caagagttat gccatctgct taataacatg ggtgcaaaca tcgagaatat
     1141 tggatcaaac acccttctca ttcaaggcgt tgacaaactt catggcggtg aatttacaat
     1201 cgggccagat tatcttgaag ttatcagctt tgttggcgcg gcagtagtta cccgtggaag
     1261 tattcacatc aagaatgctg gtgtagaaca ccttggcatg attcgactgg ttctaaagcg
     1321 cctaggtgtt gatatgcgtt ttgaaggcga tgatttaatc gtagatgcta atcaacgact
     1381 ggaaatcgaa ccggatttag gaaatgctat cccagagatt agcgttatgc cctggcccag
     1441 cttccctact gacctgatga gtatcgctat tgtcatcgca acgcaaagta aaggtacggt
     1501 actcttccat gactggatgt acccaagcag gatgttcttt acggataagc ttgtttcaat
     1561 gggcgctcaa atcgtccttt gtgacccaca tcgttgtatc gtcaatggac ctacaccatt
     1621 gactggcgaa actttggata gccctgatat tcgtgctggt acagcacttg tactcgccgc
     1681 cctatcagct gaaggcactt ctgttattcg aaatgtagct caaattgacc gtggatattc
     1741 tcagattgaa gtgaagctac gcgcaattgg cgcggatatt caacgcattt cagacaacga
     1801 ttagatattt tcttggaacc aaaaacaata aaggcacccg tttcataagg gtgccttctt
     1861 gcatattttg gtgttattta tgatttaggc tttgccagta gcaacttcaa gaaagttttt
     1921 aattgtatct cttaacatag gttccggcaa tacacctacc tgacgatgaa caatctttcc
     1981 atctttcatc atcagcatgg tcggaatacc ttgcacacca tatttacttg cccattcctg
     2041 gtgttcatcg gtatttactt tagcgatgat tagttttcca gcaaattctt tcgccatttt
     2101 atctagtata ggacgcaaca ttttacaagg cccacaccag ggtgcccaaa aatcaacgat
     2161 aacgggcaat tcgttctgca atacagtttt ttcaaatgca gcatcagtaa cattaattgg
     2221 ttcgttagtc atatttcttc ctccagatta tgacaaaagt atacagaaaa aaaattgaat
     2281 tgcaaataag agcttattca aaaccttcta tctaataaaa gacaatagtt taggtataaa
     2341 gataatgcac ccgatacaga atgccaggat agcagcaatc aaaacaaatg cggcagaaag
     2401 atcttttgct tttttggcta aaggatggat ttctggacaa atgatatcaa caattgtttc
     2461 cagggctgta tttatcaatt ctgcaatcca aaccaaggca atcgcaataa gaacagcaat
     2521 ccattccatt tgactaagtt taaaaataaa actcactgaa ataaccaaaa cagtaattat
     2581 tgcatgaatc atgacattct tttctgttct tatggcatat aaaagccctt caaatgcgtt
     2641 cttgaatgat ttgttgcgcc ctttgatata gttttgaact tctttttcag ttttgttcag
     2701 tgtcataaat taattttact ttacctggac agaaaatgcg ataaaataga gcttacgcgg
     2761 gtgttgccaa gcggtaaggc attagcttcc caagctaaca ttcgtgggtt cgaatcccat
     2821 cacccgc
//
LOCUS       DAOSFC010000189         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_34489_length_2793_cov_5.537253, whole genome shotgun sequence.
ACCESSION   DAOSFC010000189 DAOSFC010000000
VERSION     DAOSFC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34489_length_2793_cov_5.537253"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..877
                     /locus_tag="PK791_05270"
     CDS             77..877
                     /locus_tag="PK791_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH92758.1"
                     /translation="MNPDIITSSQNQHVQLMRALAGQSKARRKERAFLAEGARLVEDG
                     LQTGLVPRLVAYKEGLSPRAEESLALARSIKAKMSETFPILQIEARLFDELTDTEASQ
                     GIMAVFDIPEKELADELSFILLLDQLRDPGNMGTILRSAEAAGVQAVFLLPGTTDPWA
                     PKVVRSGMGSHFRLPILNWGIEALEALLGDMRVYYADMNGETNYWQAEFKAKTALLIG
                     GEADGASEQGRALANTTVSIPMVGGTESLNAAVSAALLMFEVARQRQV"
     gene            complement(904..1704)
                     /locus_tag="PK791_05275"
     CDS             complement(904..1704)
                     /locus_tag="PK791_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92759.1"
                     /translation="MNARRPQYAQSPKPKPFKPMAVPKAGYQAPKEPRQKSALGIILL
                     CVIFAIAIAVAVFLIKNNRPLAQPKPTITQGYIGLASHVATNPTKQQTQAIKTPTASV
                     HTDPSPTAGLTPKASQTPTRSKTPSPSPSPSASPTAENYPFIIRNSSQLAHTMYFPNE
                     KCETSIYVVGQVLDYSEKDVIGYVVKLTGVHAGKELNKSSETGSMQVFGDSGFGFVLP
                     NQIEKDDEISIQLFDPQGKPLSKKIVIEISGECDANLLLIRYKQSVVP"
     gene            complement(1701..2624)
                     /locus_tag="PK791_05280"
     CDS             complement(1701..2624)
                     /locus_tag="PK791_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOH92760.1"
                     /translation="MFKRYIAILCVLLGIVGLVLACEPLADPPELPTPVINTVAPVFE
                     PSPEITPTDLPPQLPLAAKVDGAGILLSEYERELASYRASFAETDAIPSDAEMRETVL
                     NFLIEQQLLVNAAHQNGFTLSDQALDEKIQNLSNEIGGQDALNTWMQNNQHTPESLRQ
                     SLRLAAEAAWQRDQIIAQVPDTAEQVRARQIFTIYQGDAINAERSLAGGTDFEQIAWR
                     YSPESGGELGWFPRGFLLFPEIEEAAFSLPVGATSQIIQTELGYHILYIIAHEDAHPL
                     TTDARVMLQNKALQAWLNTAREQAEIEILIP"
BASE COUNT          622 a          619 c          829 g          723 t
ORIGIN      
        1 tgcacgtttc tcataggact tttgcgcaaa gctacctaac gacaggttat aattcgtata
       61 gataatgaag gtgactatga acccagatat catcacttct tctcaaaacc aacatgtgca
      121 actgatgcgg gcattggcgg gtcaaagcaa ggcgcggcgc aaagagcgcg ctttcctggc
      181 cgaaggcgcg cgcctggtgg aagatggctt gcaaacaggc ctggtgccaa ggcttgtcgc
      241 ttacaaggaa ggtttgagcc caagagccga agaaagcctc gcgctcgcgc gcagtatcaa
      301 agccaaaatg agcgaaacct ttcccatttt gcaaatcgaa gccaggcttt ttgatgagct
      361 gaccgatacc gaagccagcc agggcatcat ggcggttttt gatatccccg aaaaagagtt
      421 agctgatgag ttgagcttta tcttgctgct ggaccaactg cgcgacccag gcaacatggg
      481 cacgattttg cgcagtgccg aagctgctgg tgtgcaggcg gtatttttgc tgccaggcac
      541 aaccgacccc tgggcgccca aggtggtcag atctggtatg ggcagtcatt tcagactacc
      601 tattctgaac tggggaattg aagcgcttga ggctttgctt ggtgatatgc gggtgtatta
      661 cgccgatatg aatggcgaaa caaattactg gcaggctgaa tttaaagcaa aaaccgcgtt
      721 gctaatcggt ggcgaggcgg atggcgccag cgagcaaggg cgcgcgcttg ctaacaccac
      781 ggtgagcatt cccatggtgg gaggcacgga gtcgctgaat gctgcggtat cagctgcttt
      841 gctgatgttt gaagtggcgc gtcaacgcca ggtttagctt ttaggggaaa ccttacgaga
      901 caatcagggg acaacacttt gtttatagcg aatgagcagt aagttcgcat cgcattcgcc
      961 actgatttcg atgacaatct ttttgcttaa cggcttaccc tgggggtcga aaagctggat
     1021 gctgatttcg tcatcttttt caatttgatt gggtaaaaca aagccaaacc ctgaatcacc
     1081 aaatacctgc attgagccag tttcgctgct tttgttgagt tctttgccag cgtgtacacc
     1141 ggtgagtttg accacatagc cgatgacgtc tttctcgctg taatccaaaa cctgaccgac
     1201 aacgtaaatc gacgtttcgc atttttcgtt tgggaagtac atcgtgtgtg ccagttgact
     1261 gctgttgcga atgatgaaag ggtagttttc ggcagttggg ctcgcgcttg ggctgggact
     1321 ggggctgggc gttttgcttc gtgtgggtgt ttggctggcc tttggggtca aaccggctgt
     1381 ggggctgggg tcggtgtgaa cagacgctgt gggggttttt atggcttggg tttgttgctt
     1441 agtggggttg gtcgcaacat gactggcaag tccgatgtag ccctgggtta tggtgggttt
     1501 gggctgggcg agtgggcgat tgttttttat cagaaagacc gcgaccgcaa tcgcaatggc
     1561 aaagatgacg caaagcagga tgatacccaa ggcgcttttt tggcggggtt ctttgggggc
     1621 ttggtagccg gctttgggta ccgccatcgg tttgaatggc tttggcttgg gtgactgagc
     1681 gtattgcggt ctgcgcgcgt tcacggaatg agtatctcga tttctgcctg ttctctggcg
     1741 gtgttcagcc aggcttgaag ggctttattc tgtagcatca ctctcgcgtc ggttgtgagg
     1801 gggtgggcgt cttcgtgggc gatgatgtaa aggatatgat aaccgagttc ggtttggata
     1861 atttgcgagg tggcaccaac tggcaggcta aaggcggctt cttcaatttc tgggaaaagc
     1921 aaaaagccac gcgggaacca accgagttcg ccaccggatt cggggctgta gcgccaggcg
     1981 atttgttcaa aatctgtgcc acctgccagg ctgcgctcgg cattgatggc gtcgccctga
     2041 tagatggtga aaatctgtcg ggcgcggact tgttcagcgg tatcgggtac ctgcgcgata
     2101 atttggtcgc gttgccaggc ggcttcggca gccaaacgca aggactgacg gagactttcg
     2161 ggcgtgtgtt gattattttg catccaggtg ttcaaagcgt cttgaccgcc tatttcgttg
     2221 ctcaggttct ggattttttc gtccagggcc tggtcgctga gtgtgaagcc attttgatgg
     2281 gcggcattga ctaatagctg ttgttcgatg agaaagttca gtacagtttc gcgcatttct
     2341 gcatcgctgg ggattgcatc ggtttcagca aatgatgccc ggtaagatgc aagttcgcgt
     2401 tcgtattcgc tcagcaaaat gcccgcgcca tcaactttag cggcaagtgg caactgggga
     2461 ggcaaatcgg tgggcgtgat ttctgggctg ggttcaaaaa ccggtgcgac tgtattgatg
     2521 accggcgttg gcaactcagg tgggtcagcc aaaggctcgc aagccaaaac caatcccaca
     2581 atgccaagca ggacgcaaag aatcgcaata tatcgtttaa acatgcctgt attatacctt
     2641 caataaggaa ttataaaatc gcagttaggc aaaatcgatg atgacacaga acacaaagca
     2701 ctgtgacaga gactaagctg gaccttatat gctcaggctt gccattctcg ggtacaattt
     2761 agagcatgaa aaccttgcgt ttttgtggtt tgg
//
LOCUS       DAOSFC010000190         2783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_34671_length_2783_cov_2.965543, whole genome shotgun sequence.
ACCESSION   DAOSFC010000190 DAOSFC010000000
VERSION     DAOSFC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34671_length_2783_cov_2.965543"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PK791_05285"
     CDS             complement(<1..703)
                     /locus_tag="PK791_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOH92761.1"
                     /translation="MENDFLKEQIHDDDYIAEVFHLKQYFPIKAGLLQRVVGHVKAVD
                     DVTFRIRKGTTLGLVGESGCGKTTVGKTMLRLNTATSGQVLFDGIDVFKLDRKGVRDL
                     RPRMQMIFQDPYSSLSPRMPISEIIGEAVREHNIVPREEHDDYIDEVMLNCGLQPYHK
                     DRYPHEFSGGQRQRICIARSLALKPDFIVCDEPVSALDVSIQAQIINLLEDLQEQKKM
                     AYLFISHDLSVVQHIS"
     gene            complement(693..1901)
                     /locus_tag="PK791_05290"
     CDS             complement(693..1901)
                     /locus_tag="PK791_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92762.1"
                     /translation="MTDENIDLKKFETTEAEEDRVKESIHLSAKESARITRENRRITN
                     AIEKRRNRRNVDPSEYLTEMKDPENVVEFDDLHTYFFTDIGTVRAVDGVSFDVPVGKT
                     VGVVGESGCGKSVTALSLMQLVQRPQGQTVKGEIRLNTGDKVYNIPETPTEVMETIRG
                     AQVSMIFQEPMTSLNPVFRAGDQINEVISLHNPEMTPEEVKKKSIDMIEMVGIANAEG
                     VYRMYPHELSGGMRQRIMIAMALACNPRLIIADEPTTALDVTIQAQILDLLRQLKDRI
                     NSSIMLITHDLGVVAEMADYVVVMYAGRIVEKGTAHEVFLNPQHPYTVGLMASKPVIN
                     KEVDRLYSIPGSVPNPINMPNYCYFRDRCNKRLPICNGEYPPEIYTTDSHMVTCYLYH
                     EQNKEANNGK"
     gene            complement(1910..>2783)
                     /locus_tag="PK791_05295"
     CDS             complement(1910..>2783)
                     /locus_tag="PK791_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH92763.1"
                     /translation="VKFKDTVRETMINKGKEFYYVDKDGEEIHYKIDVVNANYVILRE
                     TPTELVRTYEFPNKEHWLGLDNNGMDLLTRLMYGGRVSLLVGFVVIIIEMLIGVIVGG
                     ISGYFGGVVDTILMRFVDLFNSIPFYPIVVIFGSVMDALQVDSVTRIFLLMAILGILG
                     WTGIARVVRGQILSLREQDFMTATEATGIRTSRRIFRHLVPNVMPLLIVSATAGLGGL
                     IITEATLGFLGLGVKYPLASWGSIINNASSAHVMQTYWFMWFPAGMLILLTVLGFNFV
                     GDGLRDAYDPKMKR"
BASE COUNT          823 a          676 c          573 g          711 t
ORIGIN      
        1 cggagatgtg ttggacaacc gaaaggtcgt gggagataaa gagataagcc atttttttct
       61 gctcttgcaa atcctcaagc aaattgataa tctgagcttg aatggaaaca tccaaggctg
      121 acacaggctc atcacaaacg atgaaatcag gtttcagggc aagtgagcgc gcgatacaaa
      181 tacgctgacg ctgaccgcca gagaactcat gtggatagcg atctttgtga taaggctgca
      241 aaccacaatt aagcataacc tcatcgatat aatcgtcgtg ttcttcacgc gggacaatat
      301 tatgttcgcg cacagcttca ccgataatct cgctaatggg catacgtggt gaaaggctcg
      361 agtaggggtc ctggaaaatc atttgcatac gcggacgcaa atcacgaaca ccttttcggt
      421 ctaacttaaa aacgtcaata ccgtcaaaaa ggacttgccc agatgtggct gtgtttaatc
      481 gcaacattgt tttaccaact gttgtcttac cacaacccga ttcacccact aaaccaagtg
      541 ttgtaccttt tctaattctg aaagttacat catcaacagc cttaacatgg cctaccacac
      601 gttgcaacaa gcctgctttg attgggaaat actgcttaag gtgaaacacc tctgcaatat
      661 agtcatcatc atggatttgt tcttttagaa aatcattttc cattgttagc ctctttgttt
      721 tgctcgtgat aaaggtaaca ggttaccata tgggaatcag ttgtgtaaat ttcaggagga
      781 tactcaccat tacagatagg cagacgctta ttacatcgat ccctaaagta gcagtagttg
      841 ggcatgttga tggggttagg caccgaacca ggaatgctat ataagcggtc aacttccttg
      901 ttgataaccg gtttggaagc catcaaaccc accgtataag gatgctgtgg gtttaagaaa
      961 acttcatgag cggtgccttt ttcaacaata cgtccagcat acataaccac aacatagtca
     1021 gccatttcag caaccacacc caagtcgtgg gtaatcagca taatactgga gttaatgcga
     1081 tcctttaatt ggcgcagcaa gtccaaaatt tgtgcttgaa tggtcacgtc caaagcggtg
     1141 gttggttcat cggcgataat caggcgcgga ttacaagcca aagccatagc aatcattatg
     1201 cgctggcgca taccaccaga cagctcatgg ggatacatgc ggtaaacgcc ttcagcgttg
     1261 gcaataccca ccatctcaat catatcgata gattttttct taacctcttc cggggtcatt
     1321 tcagggttat gcaaggaaat aacctcatta atctggtcac ccgctctaaa cacagggttc
     1381 aaactggtca tcggttcctg gaaaatcatc gaaacctgag cgccacgaat ggtttccatt
     1441 acctcagttg gagtttcagg aatattgtaa accttgtcgc cggtgttaag gcggatttca
     1501 cccttcacgg tctgaccttg tggccgttgt accaattgca tcagagacag cgctgtaaca
     1561 gactttccgc aacccgattc acccactaca ccaacggttt tgccaactgg cacatcaaag
     1621 ctcacgccat caacagcgcg aactgtacca atatcagtga agaaataggt atgtaaatca
     1681 tcaaattcaa ccacgttctc cggatctttc atttctgtaa gatactctga agggtcaaca
     1741 ttacggcgat tgcgacgttt ttcgattgca ttcgtaatac gtcggttttc acgcgtaatt
     1801 cgtgcggact ccttcgcgct aagatggatg ctttctttta cgcgatcttc ttcagcttcg
     1861 gtcgtttcaa attttttcaa atcaatattt tcgtcagtca taagaactcc taacgtttca
     1921 tcttcggatc gtaagcgtcg cgcaaaccgt cgccaacaaa gttgaagcct aacactgtca
     1981 gcaaaatcag catgccagcc gggaaccaca taaaccagta ggtctgcatg acatgcgcgc
     2041 ttgacgcatt gttgatgatg ctaccccagg aagccaaggg gtatttaaca ccaagtccta
     2101 agaaaccaag cgtcgcctca gtaataatga ggccaccaag tccagctgta gctgaaacaa
     2161 taagcaaggg cataacatta ggtacaaggt gtcgaaagat acgtcggctg gttctaatgc
     2221 cggttgcttc agtagcggtc ataaagtctt gctcacgcaa gctcaaaatc tggccacgca
     2281 caacacgcgc gatacctgtc cagccaagga tacccaaaat tgccatcaac aagaaaatac
     2341 gcgtaaccga atctacttga agtgcatcca tcaccgagcc aaaaatgacc acaatcggat
     2401 agaagggaat gctgttaaac aagtcaacga agcgcattaa aatggtatca accacaccgc
     2461 caaagtaacc tgagatacca ccaacgataa caccaatgag catctcaatg atgataacca
     2521 caaatccgac caacagagag acccggccac catacatcaa gcgagtaagc aagtccatac
     2581 cgttgttgtc aagaccaagc caatgctcct tattcggaaa ttcgtaggtg cgaaccagtt
     2641 ccgtcggcgt ttcacgcaag ataacatagt tcgcattaac gacatcgatt ttataatgga
     2701 tttcttcccc atctttatct acgtaataga attctttccc tttattaatc atggtctcgc
     2761 gaacggtatc tttaaattta aca
//
LOCUS       DAOSFC010000191         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_34750_length_2778_cov_4.654793, whole genome shotgun sequence.
ACCESSION   DAOSFC010000191 DAOSFC010000000
VERSION     DAOSFC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34750_length_2778_cov_4.654793"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(426..1514)
                     /locus_tag="PK791_05300"
     CDS             complement(426..1514)
                     /locus_tag="PK791_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOH92764.1"
                     /translation="MEKIIALTRGVPPTQSFATEKLQACVKTALQDYPEKILQYAKAA
                     GFPPLREWVAEQYDVDASRVVIGTGSLCVLDHVLRSFPIGSLTVAIEEPTYDRTVTQL
                     KRAQAQLVPCPIEKDGLNFAPLKEKLARGEKVDFFYVVSDFQNPTGSLMPLEKRKELV
                     ALAREHGFRIIEDLPYRRLRYNGDNIPSCWEMAPDITLMMTSFSKLIAPGLRVGVMIL
                     PEELVARTLRIAEDTYINASYVDQAIVYEFCKRGWLDEQLEELKALYGHRRDVMAKAM
                     AEELSDLCDWVVPDGGFFIGAFVKKPIKMDTLLKLGKENGLQLTDGRGFYMEGGDQFV
                     RLPFCALEDDEIKEGIKRLAKVLRDYPA"
     gene            complement(1614..2312)
                     /locus_tag="PK791_05305"
     CDS             complement(1614..2312)
                     /locus_tag="PK791_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92765.1"
                     /translation="MRNPRKHISLLLFSLLLAALLFLAACERKLPDIPFSGARATYEL
                     THDPNLTLPGQENRTSTPDSSADMETETKTPESLLGVAEAVEEETQTPVKKDDSQPAA
                     TKTPSPTVERPYISFVDYWKGTWFIWFEDAGRLLEAEITFEEDGNVMVAKVEMAGHIY
                     GFTLENNGEDNVYKTYFKGTWQSDLMEKPETFNLWPQNEIQAAGYHSQPVKTFCATRD
                     KNQRPDPCFLWREE"
     gene            2487..>2778
                     /locus_tag="PK791_05310"
     CDS             2487..>2778
                     /locus_tag="PK791_05310"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOH92766.1"
                     /translation="MKQGLDLQDIIFKLQTYWAGQGCLIWQPYYSQVGAGTMNPATFL
                     RVLGPEPWKVAYLEPSVRPDDGRYGINPNRLQQFFQYQVILKPDTGNPQELYL"
BASE COUNT          700 a          713 c          604 g          761 t
ORIGIN      
        1 ttgggctcac ttctgaccca agctattgtt ttgtcaaaaa tcgcgcctcc cggcgcaact
       61 tgtgatgtag caccttggac tgattagtat agaccatttt ttggtctgtg tcaagggctt
      121 tgttctggat ttcaaaaact catttctcca gaacggtggg ttcgcttgtt tttcaaagta
      181 caacgtgcct tatttggtgc acgaacgtcc attatatgca ttttcaacaa agtgtcaagg
      241 gcaaaatcga ccaatttgtg accaatttca caaaatcacg acgttttcgc acttttttgg
      301 gtttttcaat gagcttactc atcctttaca acaaattcaa accgaacttg atttgtaaag
      361 tgcttaaaac acaagttcac cccacaggat gaacttgtca gtctcattac aaaggctgtt
      421 ttcttttagg cagggtagtc cctaagcact tttgccaggc gtttgatgcc ttccttaatc
      481 tcgtcatctt ccagcgcgca gaaaggcaga cgcacaaact gatcgccacc ttccatatag
      541 aagccacgcc catcggtgag ttgcaagcca ttctctttgc cgagtttcag gagcgtgtcc
      601 atcttaatcg gtttcttaac aaacgcgcca ataaagaaac caccatccgg cacaacccag
      661 tcgcacaaat cgctaagttc ttcggccatg gcttttgcca tcacatcgcg acggtggcca
      721 tataaagctt tcaattcttc cagctgttca tccagccagc cacgtttgca aaactcatac
      781 acaatcgcct gatcgacata gctggcattg atataagtat cttccgcaat tctcaaagtc
      841 cgcgcgacaa gttcttcagg cagaatcatc acgcccacac gcaaaccagg ggcaatcaac
      901 ttgctgaacg aagtcatcat caaagtgata tccggtgcca tctcccagca tgaagggatg
      961 ttatcgccgt tatagcgcag acggcggtag ggcaagtctt caatgatgcg gaagccatgc
     1021 tctctcgcca aagcaaccag ctctttgcgt ttttcaaggg gcatcaggct accggttgga
     1081 ttttggaaat cagaaaccac atagaaaaag tcaacttttt cacccctggc cagtttttct
     1141 ttgagtggcg caaagttcaa accgtctttt tcaatggggc aaggcacaag ctgggcctgg
     1201 gcgcgcttga gttgcgttac agtgcggtcg taggtgggct cttcaatggc gacggtcagg
     1261 gagcctatcg ggaatgaacg caaaacatgg tccaaaacac acagtgaacc cgtgccaatg
     1321 accacgcgac tggcgtccac gtcatactgt tctgccaccc attcgcgcaa gggcgggaaa
     1381 ccggccgctt tggcgtattg caagattttt tcagggtaat cctgcaaagc agtttttacg
     1441 caagcctgaa gtttctctgt cgcaaaactt tgcgttgggg gaacaccacg ggtgagcgca
     1501 atgatttttt ccatatagta ttcaatctcc tttatagctt ttttatctgc cctaatctta
     1561 gcatgaatta aataactttg ttgggatacg gaagaagcac cccaagcctg aaactattct
     1621 tcccgccaca aaaagcaggg gtcaggtcgc tggtttttat cacgtgtggc gcaaaaggtt
     1681 ttaacgggct gtgaatgata acccgccgct tggatctcat tttgtggcca cagattgaaa
     1741 gtttctggct tttccattag gtcgctttgc cacgtgccct tgaaataggt tttataaaca
     1801 ttgtcctcgc cattattctc aagcgtaaag ccataaatgt gacccgccat ctccaccttg
     1861 gcaaccatca cattgccatc ttcttcaaac gtgatttcag cttcgagcaa tcttccggcg
     1921 tcttcaaacc aaatgaacca tgtccctttc cagtagtcca caaaactgat atatgggcgc
     1981 tctacggttg gtgatggagt ttttgttgcc gcaggctggc tgtcatcttt tttgaccggc
     2041 gtttgtgttt cttcttctac agcctcagcg acaccaagaa gcgattcagg tgtcttcgtt
     2101 tctgtttcca tgtctgcaga actatccggg gtcgatgtcc ggttttcttg tccgggcaga
     2161 gttaaattag ggtcatgcgt cagttcgtaa gtcgcccgcg caccgctaaa agggatatcg
     2221 ggcaactttc gctcacatgc agccagaaac agcaaagctg ccagcaacaa actgaacaac
     2281 aataatgaaa tgtgtttccg cgggttacgc atatttttat tttactccag attcataaat
     2341 gggtcatttt catccttcat tagcgttgta aacactaaaa tgagattaaa aggctttgca
     2401 agtcgcagaa gcattcaata attgtgcttt taccggtcgt ctctgaagta taataagccc
     2461 gttatacaac attaagaggt acgaacatga aacaaggctt agatcttcaa gacattattt
     2521 tcaagctcca gacttactgg gccgggcagg gctgtttgat ttggcaaccc tactactccc
     2581 aggtcggtgc cggcaccatg aaccccgcca ccttcctgcg cgtgctgggc cccgaaccct
     2641 ggaaagtggc ttatcttgag ccttccgtgc gccccgatga cggacgctac ggcatcaacc
     2701 ccaatcggct tcaacagttc ttccagtacc aggttattct caaaccagac accggcaacc
     2761 cacaggagct ttatctga
//
LOCUS       DAOSFC010000192         2773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_34849_length_2773_cov_4.128035, whole genome shotgun sequence.
ACCESSION   DAOSFC010000192 DAOSFC010000000
VERSION     DAOSFC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34849_length_2773_cov_4.128035"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PK791_05315"
     CDS             <1..995
                     /locus_tag="PK791_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92767.1"
                     /translation="IAKDLDIGYKRGNQIEKVVSTGLNLELCPGELICLVGPNGSGKS
                     TLLRTITGLQKALAGEVLLHGKPIESYDNKALAKVISVVLTTPVRVGAMRAYDLVGLG
                     RFPFTDMFDQMREEDHKVVRTALEMAGATPLAERFVHTLSDGERQKVMIARALAQEPS
                     LLMLDEPTAFLDLPGRVSVMQLLRDLAHGHDKAILTSTHDLDLAMRIADRVWLMGRNG
                     EFLQGAPEDLALDGSFSRVFSIGKVQFDAYTGQFQMNGMPTRQVMLKAEGLNRIWAEK
                     ALNRAGFSVGDEAEIQLEFVENTWRLSYQHKQKSFENIYDLLQTLRHVELEKAE"
     gene            1226..2290
                     /gene="cobT"
                     /locus_tag="PK791_05320"
     CDS             1226..2290
                     /gene="cobT"
                     /locus_tag="PK791_05320"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOH92768.1"
                     /translation="MIEQEIMSLIIPPIPPFDEKAAEAAQARQASLTKPLGSLGKLER
                     LAVRLAGMTGKVRPRFEKKAHIIMAADHGIAEEGVSAYPQEVTPQMVLNFLSGGAAVN
                     IFARMAKAALTVVDIGVAYDFGGVEGLLDRKIAMGTQNMLHGPAMNREQAEQSIQIGM
                     DVANDLIDSGAQLLGIGEVGIGNTTPSATIIAMLSGKPVAEVTSRGTGLDDPGLAHKI
                     KVIEAVIERHKDDCGDPIAMLANVGGFELGGMAGVVLAAASRRVPVVVDGYIAGAAAL
                     IAIGLAPEAQKYLIASHESAEPGAEICTQLLGLEALFDLNFRLGEGTGSVLAFQLVEA
                     AARIQDEMATFDEAGVDEKL"
     gene            2287..>2773
                     /gene="cobS"
                     /locus_tag="PK791_05325"
     CDS             2287..>2773
                     /gene="cobS"
                     /locus_tag="PK791_05325"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOH92769.1"
                     /translation="MKSLLIAIGFLTTIPVPQIGEYEPDALGRSTAWFPIVGLLIGGL
                     VGGAYYGLGFILPAPLVAVFATIVWVALSGGLHLDGLADSFDGLLPAVSREKRLSILK
                     DVHLGAFGVIGLVLALLAKVVLLFSLPKEQLFFAIPLAAATGRWALVLAALQPSARPG
                     GL"
BASE COUNT          624 a          627 c          846 g          676 t
ORIGIN      
        1 taattgccaa agaccttgat attggctata aaagaggcaa tcagattgaa aaagtcgtca
       61 gcactgggct gaatttggaa ctttgtcctg gagagctgat ttgtttggtt ggcccgaatg
      121 gttccggtaa gtcaacactt ttgcgcacga ttaccggatt gcaaaaagcc ctggctggcg
      181 aggtcttgct gcatggcaag cctatcgaaa gctacgataa caaggctttg gccaaggtta
      241 tcagtgtcgt gctaacgacg cctgttcggg ttggcgcgat gcgcgcttat gacctggtgg
      301 gcttggggcg ttttccgttt acggatatgt tcgaccaaat gcgcgaagaa gaccacaaag
      361 tggtgcgcac cgctctggaa atggcgggtg ccacgcccct ggcggagcgt tttgtgcaca
      421 cgcttagcga tggtgaacgg caaaaagtga tgatcgcgcg cgctttggcg caggagccgt
      481 ccctgttgat gcttgatgag ccgacagctt ttttggactt gcccggacgg gtctcagtga
      541 tgcaactttt gcgcgatttg gcacacgggc acgacaaagc tatcctgact tccacacacg
      601 acctggattt ggcgatgcgt atcgcggacc gggtgtggct gatgggcaga aacggggagt
      661 ttttgcaggg cgcgccggag gatttggccc tggatggcag ctttagtaga gtcttttcga
      721 taggtaaggt gcaatttgat gcttataccg gtcagtttca aatgaacggt atgccaacgc
      781 gtcaggttat gctaaaggca gaagggctca atcgtatttg ggctgagaaa gcactcaacc
      841 gggctggttt ttctgtggga gatgaggcgg aaattcagct tgaatttgtc gaaaacacct
      901 ggcgtctgag ttatcagcat aaacaaaaaa gttttgaaaa catttatgac ttgctgcaga
      961 cattgcggca tgttgagttg gaaaaagctg agtagtgata gatataatca ggcattcagg
     1021 acttttattg aacatggtca ggagaccatg ttcgaacgcg aaccgaacgc gaacgaacgt
     1081 aaataaaacg aacgcgaata gaagcacgac cttacctaac acgttgttgc acaccaagaa
     1141 caaagtcgcg cggtgggaac accggcgaca tattaacggg ttttttcgtg gtctcctgac
     1201 cacgaaaaac acattttgaa aaataatgat tgaacaggaa attatgtctt tgattatccc
     1261 acctatccca ccatttgatg aaaaagctgc cgaggcagcg caggcgcgcc aggcaagttt
     1321 gaccaagcct ttgggttccc tgggcaagct ggaaaggctg gctgtgcgct tagccggcat
     1381 gacgggcaaa gtgcgtccgc gttttgaaaa gaaagcgcat attattatgg ctgccgacca
     1441 tggcatcgcc gaagaaggtg tgagcgctta tccacaagaa gtgaccccgc agatggtctt
     1501 aaattttctg agcggtggcg cggcggtaaa catcttcgcg cgcatggcta aggcggcgtt
     1561 gaccgtggtg gatatcggcg tggcctatga ttttggcggc gtggaaggct tgcttgaccg
     1621 caaaatcgct atgggcacac aaaatatgtt gcatgggcca gcgatgaacc gcgagcaagc
     1681 ggaacaatcg attcaaatcg gcatggacgt ggccaacgac cttatcgata gtggcgcgca
     1741 gttactgggt atcggtgagg tggggattgg caacaccacg cccagtgcga cgattatcgc
     1801 catgctttct ggcaagccgg tagcggaagt gaccagtcgt ggcacgggtt tggatgaccc
     1861 cggcctggcg cataaaatca aagtgattga agcggttatt gaacgccata aagacgactg
     1921 cggtgacccc atcgcgatgt tggcaaatgt gggcggtttt gaactgggtg gcatggcggg
     1981 cgttgtcctg gcagcggcat cccggcgcgt gccggtagtg gttgatggct atattgccgg
     2041 cgcggcggcg ctgattgcga ttggtctggc accggaagcg caaaaatatc tcatcgcgtc
     2101 gcatgaatct gctgaacctg gtgcggaaat ttgcacccag cttttgggtc tggaagcttt
     2161 gtttgacctg aacttcagac tgggcgaagg caccggatcc gtgctggctt ttcaactggt
     2221 cgaagcggcg gcacgtattc aagacgaaat ggcgactttt gacgaagcag gtgttgacga
     2281 aaaactatga aatctctgct cattgccatc ggttttctca ccaccattcc tgtgccccaa
     2341 atcggcgaat atgagcctga cgcgcttggg cgcagcacgg catggttccc gatagtgggt
     2401 ttgctgattg gcgggctggt cggtggcgcg tattatggct taggctttat cctgcctgcg
     2461 ccgcttgtgg cggtgtttgc cacgatcgtc tgggtggctt tgagcggtgg cttgcatttg
     2521 gatgggctgg cggatagttt cgacgggctt ttgccggctg tgtcgcgtga aaaacgactg
     2581 agcattttaa aggacgttca cctgggcgct tttggcgtaa taggactggt gttggcactc
     2641 ttggcaaagg tggttttact gttcagcttg cctaaagagc agcttttctt cgcgatacct
     2701 ttggctgcgg ccacagggcg ttgggctttg gttttggcag cgttgcaacc gtccgcgcga
     2761 ccgggcggtt tgg
//
LOCUS       DAOSFC010000193         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_35023_length_2764_cov_5.025840, whole genome shotgun sequence.
ACCESSION   DAOSFC010000193 DAOSFC010000000
VERSION     DAOSFC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35023_length_2764_cov_5.025840"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1422)
                     /locus_tag="PK791_05330"
     CDS             complement(208..1422)
                     /locus_tag="PK791_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOH92770.1"
                     /translation="MKRLVGTTIRGLRTPIINEGDDLLEILPNILANAAKAEGFTPRN
                     RDVLCITESVFARAQGNYAHVNDIADSVRQHFPQKPLKIAIVHPILSRNRFALLLRGI
                     AIAADKLIIELSYPTDEVGNQLIDKAELWTANINPYADVFDEESFNQKFTKYYHEFTG
                     INYIDLYREICEKEGCEVQFVFANDPRAIAGMADNIIVCDIHSRHKTRQLLKDAGAKN
                     VIGLDHILNQPSQDHGYNLEYGLLGSNKATADKVKLFPRDSQPFVEALQLRLKERFGK
                     NIEVMIYGDGAFKDPYGEIWELADPVVSPGFTSGLRGRPNELKIKYLADNDYAGLPSE
                     ELTCKIQERIREKLKAEAQKSSESSEGTTPRQITDLLGSLADLSSGSGDKGTPIVYIQ
                     GYFDNYADEYEN"
     gene            complement(1533..>2764)
                     /gene="purD"
                     /locus_tag="PK791_05335"
     CDS             complement(1533..>2764)
                     /gene="purD"
                     /locus_tag="PK791_05335"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOH92771.1"
                     /translation="SGAREHAIAWKLAQNSGVEKVFFSSHNGGATGKLENLNLPNNDT
                     QTIRAWLASHAVDLVIIGPENPLNDGIVDQLTADGVKVFGPQKAAARLEGSKAFAKAF
                     MQKYDIPTASYKECESLSSAQDALPEFGYPVVIKADGLCAGKGVYICQDESQAHQALH
                     EIFVDEVFSSQGASVVIEQYLQGFEASLLCFVSGTKIYPFDTAMDYKKIYEGDQGPNT
                     GGVGCISPNPYWTPALKAQSDAILRKIERGLEKENLGYAGILFIGYLVQDGEIYILEF
                     NTRFGDPETQVLLPRLRSDLLENIQQALAGEPVQLAFEDNVCMTTILVSDGYPKKHQT
                     GFEITGLDTLPGDIMVFHNGTKQKDGKLLSAGGRVLSVTAKRDALSDARQAVYESIKK
                     IKFDNMRYRKDIGTVSR"
BASE COUNT          635 a          634 c          752 g          743 t
ORIGIN      
        1 tgaaccgccc ggtgttaatg gtcaactgct ttgacgaagg tgaatacatc gcgcacggtg
       61 gcgcttttgt ttttgagcag ggcagggtaa gccaggaatt accgatgggt gaaaaagggt
      121 tcttgctggt tgagtgctag tttgtccaga cattaaaaaa aagggctatt tcaatgatag
      181 ccctttgtgc tttaaaatgg attgttttta attttcgtac tcgtcagcgt agttgtcgaa
      241 atagccttga atgtaaacga tgggtgtgcc tttgtcgccg gagccggagg agaggtctgc
      301 cagagagccg agcaggtcag tgatttggcg tggggtggta ccttcggagc tttcggaact
      361 tttttgggct tccgctttaa gcttttcgcg gatacgttcc tggattttac aggtaagctc
      421 ttcggaaggc aaaccggcat agtcgttatc tgcaaggtat ttaattttga gttcgttggg
      481 tctgccacgc aagcctgagg tgaatcctgg cgacacaaca gggtcagcca gttcccaaat
      541 ctcgccataa gggtctttga aagcaccgtc gccataaatc atcacttcaa tatttttgcc
      601 aaagcgttct ttgagccgga gctggagcgc ttccacaaag ggctgcgaat cacgcggaaa
      661 gagtttgacc ttgtccgcgg tggctttgtt ggaacctagc agaccatatt caaggttata
      721 gccgtggtcc tggctgggtt ggttgaggat gtgatccagc ccgatgacgt ttttcgcgcc
      781 ggcatccttg agcaactgac gggtcttatg gcgcgaatgg atgtcgcaaa cgatgatgtt
      841 atccgccatg ccagcgatgg cgcgcgggtc attggcaaaa acaaactgca cttcacagcc
      901 ttctttttcg caaatttcgc ggtaaaggtc aatgtaatta atgccggtaa actcgtgata
      961 gtatttggtg aatttttgat taaagctttc ttcatcaaaa acgtcggcat aaggattgat
     1021 gttggcagtc caaagttcag ctttgtcgat gagctggttg ccgacttcat cggtggggta
     1081 ggaaagttca atgattaatt tatccgcggc gatggcaatc ccgcgcaaca agagcgcgaa
     1141 gcggtttcgg ctgagaatgg ggtgcacaat ggcgattttt aaaggttttt gcgggaagtg
     1201 ttgcctgacg gaatcggcga tgtcattgac atgcgcgtag tttccttgcg cgcgcgcgaa
     1261 aacggattcg gtgatgcaaa gcacgtcgcg gttgcgggga gtaaagcctt ctgccttagc
     1321 tgcgtttgcc aaaatattgg ggagtatttc cagtaaatcg tcgccttcat taataatggg
     1381 cgttcttaag ccacggattg tcgtaccaac cagccttttc atagttgtcc ttgccattct
     1441 catttgtatt gtaaaagggt attggattcc gtcttttact tgcagattat agccgaaggg
     1501 gggctattca tcaaggcgaa aaccgtgatt acttatcgtg agacggtgcc aatatcctta
     1561 cgataacgca tgttatcgaa cttaatcttc tttatggatt cataaaccgc ctgacgcgcg
     1621 tcgcttaagg catcccgttt ggcagtcacc gaaagcacgc gtccacccgc gctgagcagt
     1681 ttaccatctt tttgctttgt accgttgtgg aagaccataa tgtcgccagg caaggtgtcc
     1741 aaacctgtta tctcaaagcc ggtttggtgt ttctttgggt aaccatccga aaccaaaatc
     1801 gtggtcatgc aaacattgtc ttcaaaggcg agttggacgg gttctcccgc cagggcttgc
     1861 tggatatttt caagcaagtc tgaacgaagg cggggcaaaa gcacctgggt ttccgggtcg
     1921 ccaaagcggg tgttgaattc caggatgtaa atctcgccat cctgcaccag gtaaccgatg
     1981 aaaagaatgc ccgcgtagcc aaggttttct ttctcaagac cccgttcgat tttgcgtaaa
     2041 atggcgtcgg attgcgcttt tagggcgggc gtccagtatg ggttgggcga gatgcagccc
     2101 acgccaccgg tgttggggcc ctggtcgcct tcatatatct ttttgtagtc catggcggtg
     2161 tcaaaggggt agatttttgt gccggacacg aaacacagca gggacgcctc aaagccttgc
     2221 aggtactgtt caatgaccac actcgcgccc tggcttgaaa agacctcatc gacaaagatt
     2281 tcatgcaaag cctgatgggc ctggctttca tcctggcaga tatagacgcc tttgccggcg
     2341 cagagaccat ccgctttaat aaccaccgga tagccaaatt caggcagggc atcctgggcg
     2401 gaagacaagc tttcacattc tttgtagctg gcggtaggga tatcgtactt ttgcataaaa
     2461 gctttggcaa aagctttact gccttccagc cgggcagcgg ctttttgagg gccaaagacc
     2521 ttcacgccat ccgcagtgag ttggtcaacg atgccatcat taagagggtt ttccgggccg
     2581 atgatgacca aatcaaccgc atggctggcc agccaggcac gaatcgtttg ggtgtcgttg
     2641 ttgggcaggt tgagattttc aagcttaccc gtcgcgccac cgttatggct tgaaaagaaa
     2701 accttttcaa ccccagaatt ttgagcaagt ttccaggcga tggcgtgttc gcgcgcgcca
     2761 ctgc
//
LOCUS       DAOSFC010000194         2761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_35071_length_2761_cov_6.831855, whole genome shotgun sequence.
ACCESSION   DAOSFC010000194 DAOSFC010000000
VERSION     DAOSFC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35071_length_2761_cov_6.831855"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PK791_05340"
     CDS             complement(<1..550)
                     /locus_tag="PK791_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92772.1"
                     /translation="MNTIPDTDRNQQKGRTWLYIFPILALLLLTLILMLNESPSLLEP
                     KDLVTPTDLPRASGPLEVLLFEQLPGLVSSRFTSDWRLVDVQFNEDSTQAILWMAETD
                     NNGEIFAREPLLILATLNSAKNQWSLHTALDEDFGDYLMASDFGETELADSVHIGPYV
                     KSPTGIVYGGYYLPWPAEQTKRL"
     gene            842..1093
                     /locus_tag="PK791_05345"
     CDS             842..1093
                     /locus_tag="PK791_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH92773.1"
                     /translation="MPPKGWVEINDLYCKACETCIADCPQKVLALDMSRLSIKGYHPA
                     YMAHPEKCIACGICATVCPDAAITVYRGTPPGYEEKGTE"
     gene            1096..2220
                     /gene="vorB"
                     /locus_tag="PK791_05350"
     CDS             1096..2220
                     /gene="vorB"
                     /locus_tag="PK791_05350"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOH92774.1"
                     /translation="MAKEMIKGNVAVAEAAVRAGLVSYFGYPITPQTELLEYLSARMP
                     ELGRAFVQAESELGAINMVYGAACTGLRTMSSSSSPGVSLMCEGISYIYGTEVPAVLV
                     DVMRGGPGLGNISPSQADYTQVVHGGGHGEYHLIVLAPASVQEAIDLTGEAFDLAEKY
                     RTVVVILMDGFLGQMMEPAVLPPFKPVNMESPDYAVAPKEGREKRFLTSINIDAPTQE
                     RLNIRQMSKWEEITRNEVRYQGYYLDDAEYVVVGFGTCGRIALTAVREAREKGIKIGL
                     LRPVSLSPYPTEVLDKLAEQAKGFLVVEMNNGQMLDDVVSIVARRAPVHFYGRMGGMV
                     PYPDEISQAIDEMVNSDAGPELDGRAKWLELMRETIGQGA"
     gene            2222..>2761
                     /locus_tag="PK791_05355"
     CDS             2222..>2761
                     /locus_tag="PK791_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH92775.1"
                     /translation="MTDTDVKVVYQRPIGLTDVTTHYCPGCTHGVAHRLIAEVLEEMG
                     ELRNTIGVAPVGCAVFAYNYFDFDFVEAAHGRAPAMATGIKRTLPDRLVFTYQGDGDL
                     ASMGMGETVAAAARGEQITIFFINNTNYGMTGGQMAPTTLPGQKTSSSPLGRDVETQG
                     YPIRTAEMLATLDGASYVVR"
BASE COUNT          729 a          589 c          697 g          746 t
ORIGIN      
        1 ttaagcgttt tgtttgctca gctggccaag gcaggtagta gccaccgtaa acaattcctg
       61 tgggcgattt tacatatggg ccaatatgaa cgctgtcagc taactcagtc tcaccgaaat
      121 cggatgccat aagataatca ccaaaatcct catcaagtgc ggtatgcaag gaccattggt
      181 tttttgcaga attcagtgta gcaagaatta ataatggctc ccgcgcgaaa atttcgccat
      241 tgttgtcagt ttctgccatc cacagaatag cctgggtgct gtcttcattg aattggacgt
      301 ctacaaggcg ccaatctgat gtaaaacgtg aactgactaa tccaggaagt tgctcgaaaa
      361 gcagaacttc cagtggtcca gatgcgcggg gcaagtctgt tggagtgacc aagtcttttg
      421 gctctaatag agaagggctt tcgttcagca tcaagatgag tgttaaaagc aataaggcaa
      481 ggataggaaa aatgtataac caagtgcgcc ctttttgctg atttctatct gtgtctggta
      541 tcgtgttcat agctttatcc atttaaatta aaagataatc ttcgttggat atgttgcaat
      601 ctttatgcca attgcgcgga aacgatttca ataataaatg atttatgaga tattccaaat
      661 aatgtaattt gttttttagc ttgtaggctt catgtcacga tatagtcgtc taaaatgtca
      721 tcatgtaaat ggtctgtttt gataaagtag aatgtaaaaa aaacaaaaag gtctgttata
      781 cttgttatta cctacttgga aagatcctga aaaataattt tagtatttta ggaggtaacg
      841 catgccccca aagggatggg ttgagataaa tgatttgtat tgtaaggctt gtgaaacgtg
      901 tatcgctgat tgcccgcaaa aggtcttagc actcgacatg agccgcctga gcatcaaagg
      961 ctatcatcca gcttatatgg ctcacccgga aaagtgtatc gcttgtggta tttgtgccac
     1021 tgtctgtcca gacgcagcga taaccgtcta tcgtggcaca ccgcccggat atgaagagaa
     1081 aggaacagaa taatcatggc aaaagaaatg attaaaggga atgtggcagt tgctgaagcc
     1141 gcggtaagag ctggactggt ttcttacttt ggctatccta tcacccccca aactgaactt
     1201 ttagagtatc tttccgcgcg aatgcccgaa cttggtcgcg cttttgtgca agctgaaagc
     1261 gaacttggcg caatcaatat ggtttatggc gcggcttgta ccggactgcg caccatgagt
     1321 tcaagttcaa gtcctggtgt tagcctgatg tgcgaaggta tttcatatat ttatggtaca
     1381 gaggttcccg ctgtattggt tgacgtcatg cgcggtggcc ctggcttggg aaatatttca
     1441 cccagccaag ccgattacac gcaagttgtg catggtggcg gtcatggtga gtatcatctc
     1501 atcgttttgg cacccgcttc agtacaggaa gcgattgacc taaccggcga agcatttgat
     1561 ttagcagaga aatatcgcac cgtggttgtc attttgatgg atggtttcct gggccagatg
     1621 atggagcccg cagtgctgcc acccttcaaa ccagtcaata tggaatcgcc agattatgcg
     1681 gtcgctccca aagaaggacg cgagaagcgc ttcctgacgt ccatcaatat tgatgcaccc
     1741 acgcaagaac ggctgaacat tcgccagatg agcaaatggg aggaaattac ccgcaatgaa
     1801 gtccgctacc aaggctatta cctggatgac gcggagtacg ttgtggttgg ttttggtact
     1861 tgtggacgta tcgcgctaac tgctgtgcgt gaggcccgtg aaaaaggcat caagattggt
     1921 ttgttgcgcc cagtaagcct ttcgccttac ccgacagaag tgcttgataa actcgctgag
     1981 caagccaaag gctttttggt tgtcgaaatg aacaatggcc aaatgttaga tgatgttgtc
     2041 tcgattgttg ccagacgcgc gccagtgcat ttttatggcc gtatgggtgg tatggtgcct
     2101 tatcccgatg agattagcca agctattgat gaaatggtta acagcgacgc tggacccgag
     2161 ttggatggcc gtgccaaatg gcttgaactc atgcgtgaaa caataggaca aggagcataa
     2221 gatgactgat actgatgtaa aagttgttta tcaacgcccg ataggtctaa ccgacgttac
     2281 aacacactat tgccctggtt gtactcatgg cgtggcacat cgcttaatcg ctgaagtgct
     2341 tgaagaaatg ggcgaattac gcaacacaat cggtgtggca ccggttgggt gtgcggtgtt
     2401 tgcctacaat tattttgatt ttgattttgt agaagctgca catggccgtg cacctgcgat
     2461 ggcaaccggc atcaagcgta ctctgcccga tcgcttggtt ttcacctatc aaggtgacgg
     2521 tgacctggct tcaatgggta tgggcgaaac cgtggcagct gccgcacgtg gtgaacagat
     2581 tacaatcttc ttcatcaaca ataccaacta cggtatgacc ggtggtcaga tggcacccac
     2641 gacactgccc ggacaaaaga caagctcatc acctttaggc cgtgatgtgg aaactcaggg
     2701 ttatccgatc cgtaccgctg agatgttggc aacacttgat ggtgccagct atgtcgtgcg
     2761 t
//
LOCUS       DAOSFC010000195         2750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_35263_length_2750_cov_4.905751, whole genome shotgun sequence.
ACCESSION   DAOSFC010000195 DAOSFC010000000
VERSION     DAOSFC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35263_length_2750_cov_4.905751"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1624)
                     /gene="dnaA"
                     /locus_tag="PK791_05360"
     CDS             complement(233..1624)
                     /gene="dnaA"
                     /locus_tag="PK791_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOH92776.1"
                     /translation="MDAQQAWKATMTQLKNELPKASFDTWVKQCVFIGWDEMTSTMTI
                     GANNAYACDWLESRLKSTVVNKLRGMMALELNVVFKVHNAPVLRPVEEEPAPEDLLTA
                     GIDNDRVQIDGAETYRSLRQDSQLNPKYRFDNFVVGPGNRLAHAASMAVAEHPANAYN
                     PLFIYSGVGLGKTHMLHAIGNAVVRQGKTVLYVSSEEFTNDLVGAIRNKTTTAFREKY
                     RRVDVLLIDDIQFIAGKESTQEEFFHTFNTLYGQDKQIVISSDRSPKAMSMLEERLRS
                     RFEWGLTVDMQAPDYETRLAILQSKAEKEGRVVQTEIMELIAKTIQSNIRELEGAWNR
                     VLAFSDLTGKQLTLQMAQNALADYAPQPSSLTPDVVVDVVADYFGIDPEKLSGRSRAR
                     EVALPRQVAMYLLREDSGISLPRIGKELGGRDHTTVMYACDKVAELIDSDDMMRRKVS
                     QIRERLHNNKVLV"
     gene            complement(2082..>2750)
                     /locus_tag="PK791_05365"
     CDS             complement(2082..>2750)
                     /locus_tag="PK791_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH92777.1"
                     /translation="VSLRYNTRATQLVRQADGKIQVSLENAKPETFDQVLVTLSPHAM
                     AKMTPDLPEDYLAGLLNLKHMGAVVMTVSLKHALSPEGYYWYNIPKQAGYPFLSLVEH
                     TNFLKPEYFGGDHIIYIGDYLETDHPYFSKTDDELAAEFLPHLKKINPNFSEDWVRKV
                     WVSKAEYAQPVPLVNHSQAIPSIQTPIEGIYFASMSQVYPWDRGTNFAIDIGRKSAQL
                     MMGL"
BASE COUNT          689 a          686 c          621 g          754 t
ORIGIN      
        1 ttatggaatc agccatgacg ctggatgtgc cccttgtctg cgatgcccgc gccggcgata
       61 actggcgtga gctgaaggct gtttaacgcg ttcgcgtttg ctctgacacg cgttcgcgtt
      121 tgtaaccctt gaattaaagc attcacggtg tggtcgttac tcagaaccac accgtgaata
      181 attgtccgtt ttccctccca gaataagacc aaaaataaac ctatattaac aattaaacaa
      241 gcaccttatt attatgcaac ctttcgcgaa tttgggaaac tttccgacgc atcatatcat
      301 cactatcaat cagttcagca accttatcgc acgcatacat taccgtggta tggtcacgac
      361 cgcccaactc tttgccgatg cgcggtaaag aaataccact gtcctcgcgt aaaaggtaca
      421 tcgcaacctg acggggcaaa gccacctctc tcgcgcgcga ccgtcccgaa agtttctcag
      481 ggtcgatacc aaagtaatcc gccaccacat ccacaaccac atcgggtgtc aggctgctgg
      541 gctgtggcgc gtagtcggca agcgcgtttt gcgccatttg caaggtgagt tgcttgccgg
      601 tcaggtcaga aaaagccaac acacggttcc acgcaccttc aagttcgcgg atattactct
      661 gaatggtctt ggcaatcaat tccataattt cagtttgaac cacccgtcct tctttttcgg
      721 ctttgctttg caaaattgcc aggcgtgttt cgtaatcagg tgcttgcata tccaccgtca
      781 agccccattc aaagcgtgag cgcaggcgtt cttccagcat actcatcgct tttggcgaac
      841 ggtcggagct gatgacgatt tgtttgtctt gcccgtaaag ggtgttgaag gtgtggaaaa
      901 attcttcctg ggtcgattcc ttgcccgcaa taaactggat gtcatcaata agcagcacat
      961 ccacccggcg atatttctcg cggaaagcgg tagtggtttt gttgcggatt gcgccaacca
     1021 aatcgttggt aaattcttca gatgaaacat aaagcaccgt cttgccctgc cgcaccacag
     1081 cgttaccgat ggcgtggagc atatgtgttt tgcctaatcc cacgccactg tagatgaaca
     1141 ggggattata ggcgttggcg ggatgttcgg caacggccat gctggcagcg tgtgccaggc
     1201 ggttgcctgg cccaaccaca aagttgtcga aacggtattt gggattcagc tgactgtcct
     1261 gacggagcga acgataagtt tccgcgccgt caatctgaac tctatcatta tcaataccag
     1321 ctgttaaaag atcttccggt gctggttctt cttccacggg tctcaaaact ggcgcgttgt
     1381 gcactttaaa gaccacatta agctctaatg ccatcatgcc tcgcagtttg ttgaccacgg
     1441 tgcttttcag gcgactttcc agccagtcgc aagcataggc attgttggca ccgatggtca
     1501 tcgtgctggt catttcgtcc cagccgataa acacacactg cttcacccag gtatcaaaag
     1561 atgctttggg taattcattt ttcagttgtg tcatcgttgc cttccatgct tgctgagcat
     1621 ccatggcttt ctcctgtgcg ccgtcactgg cagtctttga ttttaagggt cgtttaacta
     1681 ggcgcgtcaa tttcctctac tttagacgcg ctgaccgatt gaatataaat tgtctggttg
     1741 tttaaatcgg tagccctatt ctaacacttc gcgcgcaaaa aatgtctctg gaaaccttaa
     1801 aaaatgacaa ttctttaaga ttgtaagact cttaaaactt atgctttttc tgataagtat
     1861 tctactttat acctaacaag ctcccatagc ccctatttac agggttcaaa agcttttaca
     1921 acctgaatat gtggggtttt aggtcatttt tcgaacattt taccgcttta tagggggtgt
     1981 cttttaaacc tcaaaaaacg tttcacgagt tacagaaact gagatagtga ttcacaaaat
     2041 ggaatcagta cttaacaaat actttacaaa tgcttaatcg tttacaagcc catcatcagc
     2101 tgagcagact tacgcccaat atcaattgca aaattcgtgc cacgatccca cggataaacc
     2161 tgactcatac tggcgaagta aataccctca atcggggtct gtatagatgg aatagcctga
     2221 ctgtggttta caaggggcac gggctgggca tattctgcct ttgaaaccca aaccttcctg
     2281 acccagtctt cgctaaaatt cggatttatc ttcttaaggt gcggtaaaaa ctcggctgcg
     2341 agttcgtcat cggtcttgct aaagtaggga tgatccgtct caagataatc accgatgtaa
     2401 ataatatggt caccaccgaa gtattcaggt ttcaggaaat ttgtatgctc gactaaagac
     2461 aggaaaggat agcccgcctg cttgggaatg ttgtaccaat agtatccttc gggcgaaagg
     2521 gcatgtttga gcgacacagt catcaccacc gcgcccatat gcttgagatt gagcaaacca
     2581 gccagatagt cttcgggcaa atcaggcgtc attttcgcca tggcatgtgg tgatagcgtg
     2641 accaaaacct ggtcaaaagt ttcgggcttg gcattttcaa gactgacttg tatctttcca
     2701 tccgcctgac gaaccagttg cgtcgcgcgg gtgttatagc gcaggctcac
//
LOCUS       DAOSFC010000196         2738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_35496_length_2738_cov_6.750280, whole genome shotgun sequence.
ACCESSION   DAOSFC010000196 DAOSFC010000000
VERSION     DAOSFC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35496_length_2738_cov_6.750280"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK791_05370"
     CDS             <1..396
                     /locus_tag="PK791_05370"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOH92778.1"
                     /translation="SECQAYDQLARDMGGMDLQILGLGTTGHIAFNEPDSTFHPFTRL
                     VNLDKSTMAANGHFFKTNEAVPHQAVTVGMRFILNSRLILLLVSSDKKAEALERTLNG
                     PIDPMVPASILQLHPNVIIIADAEARSKL"
     gene            complement(472..1653)
                     /locus_tag="PK791_05375"
     CDS             complement(472..1653)
                     /locus_tag="PK791_05375"
                     /inference="COORDINATES: protein motif:HMM:NF022167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92779.1"
                     /translation="MSENTQIESNPLQTILEEFPRQKPSPAHDMDQEEVLRVLELAEI
                     ASVPLVVAGGWAVDALLGWQTRPHGDLDLAINRAHLVRFLGILSRIGYGFVNEVHAND
                     KSYILDNGVGHQIEIHVYTRDDKGRIISGSRYPSNSLSGQGKIGHLKVSCVAPEWLID
                     SYTDKEINEKTLQDLKYISNLFRLELPIEFKEYDDSLYSQHAQKYRVLPSDFELIEPK
                     TGHDYSELVLCLADVDQQIAEDVRQNEAARFEKVFMETAPNPLTAANFDLCTGLLLKR
                     NDELVGAIELGPVRSALTEAGEEGKIWQIKQIRMGQFAEYARIFQYIIKEASKWACEH
                     GANQLRVAPLDDISTNRPAHLKEYDFAHIGYATKYEQIGFEPLTGETLQRLVLVKRLD
                     E"
     gene            complement(1783..>2738)
                     /locus_tag="PK791_05380"
     CDS             complement(1783..>2738)
                     /locus_tag="PK791_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92780.1"
                     /translation="PVIDQLAIDYAERNIVFVDYTMDWGIPNPVLYRYEVIERTEPGW
                     GLTWAMIDSGQRYSRGAETHDEAVEKYTAMVEYGIAQEALADISAQYWVIDDTAYFKV
                     TVQNLSGVDLSQENSARVYAFVKETGIQYEDTNTTHHASRGEGMTLIPNLADQETGTY
                     YVSVPLREITDWNNVGAYAFVDYEYSTGGLFLQLNATEATKIDFVPVLTVGPTRITLT
                     IPDNLTEIPPQDIRIMAGSEISWVIETNRPWINVNVEEGTGPAGIVLDINAFTLRKGN
                     NRATLLVKDINSDSFASVEIDILVEAKAPPPYSLYIPLLTK"
BASE COUNT          748 a          621 c          538 g          831 t
ORIGIN      
        1 tctgaatgtc aggcttatga ccaacttgcc agggatatgg gggggatgga cttgcaaatt
       61 ttaggcttag gcaccactgg acatattgct ttcaatgaac cagatagcac cttccatccc
      121 tttacacgac tggttaattt ggacaaatcc acaatggctg caaacggtca ctttttcaaa
      181 accaatgaag cggtaccaca tcaggctgtt acggttggta tgcgtttcat tctcaatagc
      241 cgattaatcc ttttgcttgt aagttcagat aaaaaagctg aagcccttga gcgaaccctt
      301 aacggcccga ttgatcccat ggtacccgca tcaattctgc aacttcatcc aaacgtcatt
      361 attatcgctg atgcagaagc aagatcaaag ttgtaagcaa aaatttaact tccaatgttt
      421 aacgattaaa aaactctcga tatgagagct ttttttctgt tctttgagta attattcgtc
      481 aagccttttt accaatacaa gtctttgtaa tgtttcacca gtaagtggtt caaagccaat
      541 ttgctcatac ttggtggcat atccgatatg cgcaaagtcg tattctttca aatgggcagg
      601 ccggttggtg ctgatgtcat ccaatggcgc gacgcgcaat tggttagcac catgctcaca
      661 agcccatttg ctagcttctt ttattatata ttgaaatatc cttgcatatt cagcaaattg
      721 acccatgcga atttgtttaa tctgccagat tttgccttct tcacctgctt cagtcaatgc
      781 agagcgaacg ggcccaagtt cgattgcgcc aacaagttca tcatttcgct ttaaaagcaa
      841 gccggtgcac aaatcgaaat tcgccgcagt aagtgggttg ggtgccgttt ccataaaaac
      901 tttttcaaat cgagccgcct cattttgtcg cacatcttct gcaatttgct gatccacatc
      961 agctaagcat agtacaagct cagaataatc atgccctgtt ttcggttcga taagttcaaa
     1021 atcggaaggc aaaacacgat atttttgcgc atgttgggaa tacaagctgt catcatactc
     1081 tttaaattca atcggcagtt ccaaacggaa caaattgctt atgtacttca gatcctgtaa
     1141 ggtcttttcg ttgatttctt tgtcagtata ggagtcaatc agccactcag gcgcaacaca
     1201 actaactttt aagtgtccga ttttgccttg tccactgagc gaattgcttg gatagcgtga
     1261 accactaata atgcgtcctt tatcatcgcg cgtataaaca tgaatttcaa tttggtgtcc
     1321 aacgccatta tccaatatat agcttttgtc attggcatgg acttcgttca caaagccata
     1381 accaatcctg ctcaatattc ccaaaaatcg cactaaatga gcacggttta tcgccaggtc
     1441 aaggtcgcca tgcggtcttg tttgccaacc caaaagcgca tcaaccgccc aaccacccgc
     1501 aaccaccagt ggaacgctgg ctatctcagc tagctcaagc acgcgcagga cttcttcctg
     1561 gtccatatca tgagctggtg atggtttttg gcgtggaaat tcctctagta ttgtttgcaa
     1621 gggattgctt tctatttgtg tgttttcact cattgcgcgc tccttggatc tttgtataat
     1681 tgtaccgttt aagaagaaaa ctgtatagtt ttttatgaat aactaaagct tttttatgaa
     1741 aaaagccccg tgtgtacggg gctttgtgcg tagtttaggc ttttacttcg taagaagtgg
     1801 tatatagagc gaataaggag gcggcgcttt tgcttccact aaaatatcaa tttcaacact
     1861 ggcgaaagaa tcgctgttta tgtccttcac caacaatgtc gcccggttat ttcctttacg
     1921 taaagtaaaa gcgttaatgt caagaacaat tccagcaggc cctgttcctt cttcaacatt
     1981 aacattaatc catggacgat tggtttcaat cacccatgaa atctcagacc ctgccattat
     2041 tctaatatct tgaggcggga tttcggttaa attgtcagga atagtaagtg taattcgcgt
     2101 tgggccaacc gttaatacag gcacaaagtc gatttttgta gcttctgtgg cattcaactg
     2161 caagaaaagt ccacccgttg aatattcgta atcaacaaaa gcataagcac caacattatt
     2221 ccagtcagta atctcgcgta gtggaacaga cacataatag gtgccagttt cctgatctgc
     2281 taaattagga ataagggtca taccttcacc acgtgaggcg tgatgggttg tgttcgtatc
     2341 ttcgtattga ataccggttt cttttacaaa ggcgtatact ctggcagagt tttcttgact
     2401 taagtctacg ccactcaaat tttgaaccgt tactttgaaa taggctgtgt catctatgac
     2461 ccaatattgg gcacttatat cagcaagcgc ttcctgagcg atgccgtatt ccaccattgc
     2521 agtgtatttt tcgacagctt catcatgtgt ttctgcacca cgggaatatc tttgtccact
     2581 gtcaatcatg gcccaggtca atccccaacc aggttcggta cgttcaataa cttcataccg
     2641 ataaagtact ggattaggaa taccccaatc catagtgtag tcgacaaata caatatttct
     2701 ttctgcgtag tcgatagcta attgatcaat caccggac
//
LOCUS       DAOSFC010000197         2724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_35770_length_2724_cov_4.892094, whole genome shotgun sequence.
ACCESSION   DAOSFC010000197 DAOSFC010000000
VERSION     DAOSFC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35770_length_2724_cov_4.892094"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PK791_05385"
     CDS             complement(<1..1239)
                     /locus_tag="PK791_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH92781.1"
                     /translation="MTDGLNMQTCPKCGKENLAYMTHCVHCGSALDEMFSFEGQIDLP
                     DDALEESATTLPFVLEEMQSGAGLLPDDEPELEPAKAKEDSDTEDVPDWLQRVRQRAR
                     EEEDATGELTKGIQSMQDMVVGEEKDDSESQYQDWIETVREKTQREKAKRAERLRPSP
                     LDEQGVPEWLRRIRALNPQDEEMLEEESVVDEWTDEALEDLRRTRLGDDYTPALLPDD
                     TETEEESEDSPEETEEPKIEHNIPEDDEDLLSLESDASDGAETGDSQDISSGAGDAEK
                     DLGDTLSEHTGFVIVGEPKLKPDEVFDTKPPEMEPAQTEPDANAKKRLREEEKEAILQ
                     DLVILRGQHEKVALLKSMIASEGQPIPNATESKPKKKDLSRLIVGLAFILILLLAIIV
                     IPRHKTDSVPAGFVAAKNRFD"
     gene            complement(1232..1846)
                     /locus_tag="PK791_05390"
     CDS             complement(1232..1846)
                     /locus_tag="PK791_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92782.1"
                     /translation="MKKIVPLIVATVTTLFVMAGIFFRESLGGWLSVVLNWAIVLSSV
                     ALLVVLARLLFTHIAYIARGRQGFIYSLVAVSAFLITLIAGFLFGVDNPTFLKWIAGI
                     MRPLESALLGLVAIVLASLSLKFFYQRGWNALTISFAVSALGFLFLGLGVLQAIDYPP
                     LQRVIQAIEGLPMIGARGLLIGVTLGAILMGMRVLIGAERPYDD"
     gene            complement(1843..2607)
                     /locus_tag="PK791_05395"
     CDS             complement(1843..2607)
                     /locus_tag="PK791_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92783.1"
                     /translation="MPELSLNDWLAYGTLAGLFLIFIISYFIARAKAAQNKASAKENT
                     RLDAILHRSSERGEGVLVGTSEGAGAQVGSLAGLAGLQTQAHVFRHSLHSDLPPKTVA
                     GDGNLVALSQQLNAGIYHTADMPELYKLDSSGLAGIGSYAYLAGLIPELSRPNLSALL
                     MQGELSPELILALDAAKRHDLDAIMASSSLSGQAAAHFIDPQSALGEEAFMSQSTRPR
                     EKAGLKATLICLRIARGLIIAGLLVAILLQLLGVLP"
BASE COUNT          672 a          783 c          613 g          656 t
ORIGIN      
        1 gtcaaagcgg tttttcgccg cgacaaaacc cgcgggcaca gaatcggtct tatgacgggg
       61 aataacgata atagcaagca gtaaaatcaa tatgaatgcc agcccgacaa tcaggcggct
      121 aaggtctttt ttcttcggtt tgctttctgt ggcatttgga atcggctgcc cttccgaagc
      181 aatcattgat tttaacagcg cgactttctc atgctgtccg cgcagaatga ccaggtcttg
      241 cagaatagcc tctttttctt cttctcttag gcgttttttg gcgttggcgt ctggttcggt
      301 ctgagcaggc tccatctccg ggggtttggt gtcaaaaact tcatcaggct tgagtttcgg
      361 ttcgccgaca atcacgaagc cggtatgctc gctcagggta tcacctaaat ctttttcagc
      421 atctcccgct ccgcttgata tatcctgtga atcgccagtt tccgccccat cggatgcgtc
      481 gctttccagg ctcaagagat cctcatcatc ttcggggata ttatgctcaa tcttaggctc
      541 ttccgtttcc tctggactat cctcggattc ttcttctgtt tcagtatcgt caggaagtaa
      601 agcgggtgta tagtcatcac ccagtcttgt acgacgtaaa tcctcaagcg cctcatcggt
      661 ccactcatcg acgacgcttt cttcttcaag catttcctca tcctggggat tcaacgcccg
      721 aatacgccgc aaccactccg gcacgccttg ctcatccagc ggactgggac gcagacgctc
      781 agcgcgtttg gccttttcgc gctgggtttt ctcacgcaca gtttcaatcc agtcctgata
      841 ctgactttcg gagtcgtctt tttcctcccc gaccaccata tcctgcatgc tttgaatacc
      901 cttggtcagt tcaccggtag catcttcttc ctcccgcgcg cgctggcgca cacgttgcaa
      961 ccagtcaggc acgtcttcgg tatcggaatc ttctttggct ttggcaggct caagttcagg
     1021 ctcatcatca ggcaacaaac ccgcgcctga ctgcatttct tcaagcacaa aaggcaaagt
     1081 cgtcgcgctt tcttccaggg catcatcagg aaggtcaatt tgcccttcaa aactgaacat
     1141 ctcatccagc gcgctgccac aatgcacaca atgcgtcatg tacgccaggt tttctttgcc
     1201 acatttggga caagtttgca tatttaagcc gtcagtcatc gtaaggcctt tctgcgccaa
     1261 tcaaaacgcg catgcccatc aaaatcgcgc ccaaagtcac gccaatcagc aagccacgcg
     1321 caccgatcat cggcaatcct tcaatcgcct gaataacgcg ttgcaaagga ggataatcaa
     1381 tcgcttgcaa aacgcccagt cccaaaaaca aaaaacccag ggcgctaacc gcgaaagaaa
     1441 tcgtcagcgc gttccagccg cgttgataaa agaacttaag gctcaaactc gccaaaacaa
     1501 tcgctaccag gcccaaaagc gcgctctcca gcggacgcat aatgccggca atccatttta
     1561 gaaaagtggg attatccaca ccaaacaaaa aaccagcgat tagggtaatc aaaaaggcgc
     1621 tcaccgcgac caaactgtaa ataaaaccct gtctaccacg cgcgatataa gcaatgtgcg
     1681 tgaacagcaa acgcgccagc actaccaaaa gtgccacgct cgatagcaca atcgcccagt
     1741 ttagcaccac actcagccag ccacccaggc tctcgcgaaa gaagatgccc gccatcacaa
     1801 aaagcgtggt tacggttgcc acgattaaag gcacaatctt cttcatggca aaacccccaa
     1861 gagttgcaac agaattgcta caagcaggcc cgctataatc aaaccacgcg cgattctaag
     1921 gcagatgagg gttgccttaa gccctgcttt ttcacgcgga cgggtggatt gactcataaa
     1981 agcttcttcg cccaacgcgc tttgtgggtc aataaagtgc gccgcggcct gtccggaaag
     2041 tgaactgctc gccataatcg cgtccaggtc atgacgtttg gcagcgtcaa gtgcaagaat
     2101 aagctcaggg ctcagctcgc cctgcatcag caaggcactc aggtttggcc ggctcaattc
     2161 cgggataagt cccgccaaat acgcgtaact gccaataccc gccagaccgc tactatcaag
     2221 cttataaagt tctggcatat ccgcggtgtg ataaatgccg gcatttaatt gttggctaag
     2281 cgctaccaag ttgccatcgc ccgccaccgt tttgggtggc aggtcgctgt gcaaactgtg
     2341 ccgaaaaacg tgcgcctggg tctgcaaacc cgccagtcca gccagtgaac ctacctgcgc
     2401 gccggcacct tcgcttgtgc caaccagcac accctcaccg cgttctgaac tgcggtgcag
     2461 gatggcatcc aggcgagtgt tttctttggc tgaggcttta ttttgcgctg cttttgcgcg
     2521 cgcgatgaaa tagctgatga taaaaatgag aaacaaaccg gctaaagtgc cgtaggcaag
     2581 ccagtcattc agacttaatt caggcatttc gcccccgttg ctttagatat acaccgcatt
     2641 gcatatctgt tttttccatg gataaagtat agcatgatta tgcctggcta cacaaaaata
     2701 tttccgtaat gtaaattatg ggta
//
LOCUS       DAOSFC010000198         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_36865_length_2668_cov_7.446230, whole genome shotgun sequence.
ACCESSION   DAOSFC010000198 DAOSFC010000000
VERSION     DAOSFC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36865_length_2668_cov_7.446230"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PK791_05400"
     CDS             complement(<1..1166)
                     /locus_tag="PK791_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOH92784.1"
                     /translation="MIILAYDIGTTGVKTCLFQLIESDSPEGMSINLEDSNFASYSLS
                     ILPNGGVEQNPQEWWDAMCKTTRQLVSHNPETCAQIAGISFCAQAQSVVLLDQAARAV
                     RPSMSYMDQRSGGVRKAHAGGGPQVAGVGLGFLLQSLYHTGVVAGSDKDPVWKYLWVK
                     ENEPEVYARAHKWLDVKDALVARMTGKFTMSRDSAFATLLMDKNTKTPEFNAALIKRL
                     GILPEHMPEIVESTAKVGTLLEEPATELGLSPGIPVFSGGSDAALIGVGAGCVRPEMT
                     HVYMGTSGWVSTVTDQNIVDTNAMIASVVGVQPGFYNYFAELETAGKCLEWVRDHLAL
                     DEINIYLSKQHVADDPSSEYKSLYDYMSKVISETPAGSGGVIFTPWLHGNRCPF"
     gene            complement(1184..1849)
                     /locus_tag="PK791_05405"
     CDS             complement(1184..1849)
                     /locus_tag="PK791_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH92785.1"
                     /translation="MNNFANESFKRLNPEKQERILKAAIAEFADRGYELANTNRIAKR
                     AKISVGSIFHYFNTKENLFLYIVQYGSALIETHAGKVVDDETLSVAQKIEALVRLVVR
                     TSREEITLIRLYHELSSIGNQELISKLPRSMEQFTSERYIKMLEEGQRSGEVKPELDA
                     RLAAFSMDNILLSLQFAYACDYYRIRFQMYNNPEIDQEEYDEQVISETFQFLKGALLV
                     AED"
     gene            2144..>2668
                     /locus_tag="PK791_05410"
     CDS             2144..>2668
                     /locus_tag="PK791_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH92786.1"
                     /translation="MIDLSSATIRKQTEALDELIKKPIHSISPDALATYETEYFGKKC
                     VKSYEMIQEAKKYIPGGVQHNLAFNHPFPLVFTKAEGAYLEDLDGNRYFDFLQAGGPT
                     VLGSNPPEVREKVIELLNTCGPSTGLFHEYEYRIAKLVTELIPSVTWLRMLNSGTEAC
                     MAAIRVARLATKHRH"
BASE COUNT          706 a          692 c          582 g          688 t
ORIGIN      
        1 tcaaaaggac agcggttgcc gtggagccac ggggtgaaaa tgacaccgcc ggaacccgcg
       61 ggcgtttcgg aaatgacctt ggacatatag tcgtacaaac ttttatactc cgaactcgga
      121 tcatccgcga cgtgttgttt gctgagataa atgttaatct cgtccagagc aagatggtcg
      181 cgcacccact ccaggcattt gcccgcagtc tcaagctccg caaagtaatt gtaaaaacca
      241 ggttgaacac ccacgaccga ggcaatcatg gcatttgtgt ccacgatatt ttgatcggtg
      301 accgtggaga cccaaccgga cgtacccata tagacatggg tcatttcagg acgcacgcaa
      361 ccagcgccga cgccaatcag agccgcatca ctgccaccgg aaaagacggg aattccgggg
      421 cttagtccaa gttcagtggc gggttcttcc aaaagcgtgc ctacttttgc tgttgactca
      481 acgatttcgg gcatatgctc aggcaaaatc ccaaggcgtt tgattagcgc ggcgttaaat
      541 tcaggcgttt ttgtattttt atccatcaac aaggtagcaa aagcggaatc ccgactcatg
      601 gtaaatttac ccgtcatccg tgccaccagg gcatccttta cgtccagcca cttgtgcgcc
      661 ctggcgtaaa cttccggttc gttttctttc acccaaaggt acttccagac cgggtccttg
      721 tcgctgccag caacaacacc cgtgtgataa agactctgca aaaggaaacc caaaccaaca
      781 ccggcgactt ggggaccacc cccagcgtgt gctttgcgta ccccacccga gcgttggtcc
      841 atataactca ttgacggacg cactgcgcgg gctgcctggt caagcagaac cacgctctga
      901 gcctgggcac agaagctgat gcccgcaatc tgcgcacatg tttcagggtt atggctgaca
      961 agctgtcgcg ttgtcttgca catggcatcc caccactcct gtggattctg ctcaacacca
     1021 ccgtttggaa gaatgcttag ggaatagctt gcaaagtttg aatcttccaa attgatagac
     1081 atcccctccg ggctgtcact ctcaattaat tgaaataagc aggttttaac ccctgttgtg
     1141 ccaatgtcat acgccagaat aatcattgtt ccagcccctt tcgttaatct tcagctacca
     1201 agagtgcccc tttcaaaaat tgaaaggtct ccgaaatgac ttgctcatca tattcttcct
     1261 ggtcaatctc cgggttgtta tacatttgga agcgaatcct gtaataatcg caggcataag
     1321 caaactgtaa tgacaacagg atattatcca tcgaaaaagc agcgagcctg gcatccagtt
     1381 ccggcttaac ttcaccgctt cgctgacctt cttccagcat ttttatataa cgttctgacg
     1441 taaactgctc catacttcgt ggcagtttag atattagctc ctggttgcca atcgaggaca
     1501 actcgtgata caggcgaatt aaagttattt cttcccgaga cgttctaacc accaggcgca
     1561 ctagagcttc aatcttctga gcgacactca atgtttcatc atcgaccact tttccggcat
     1621 gtgtctcaat cagtgctgaa ccgtattgaa cgatataaag gaagagattc tctttggtat
     1681 tgaaatagtg aaaaatcgac cccacactga ttttagctcg cttggcaatg cgattagtgt
     1741 tggctaattc ataaccccgg tctgcaaatt cagcaatggc agcttttagt attcgttcct
     1801 gtttttccgg attcaggcgt ttgaaactct cgttggcaaa attgttcatc ttaatccatg
     1861 tccaattatt taatttgata ctctgccgtc tttgaatatg gaaagcgtat tcaacaacgg
     1921 agaagaaggt tttcggaagg agacctacat actttaaaca atattgacgg gctccgtgtt
     1981 gagtgaccac tcaacacctt gtgccaatat taccacatta actaaaaaaa cataacgttt
     2041 tctcccgcat tcaaaaaatc ccttgcgctt tggggctggc cgtggtaaat ttatattgag
     2101 tggccactca attttgagat gtggtcacaa tatggaggtc gtcatgatag atttaagttc
     2161 cgcaacaatc agaaaacaaa ccgaagcact ggacgagctg attaaaaagc ctatccattc
     2221 catctcccct gatgcgcttg ctacatatga aacagagtat ttcggcaaga agtgtgtcaa
     2281 aagctacgag atgatacagg aagccaagaa atatattccc gggggcgtgc aacacaacct
     2341 tgctttcaat catccctttc ctctcgtttt caccaaagcg gaaggcgctt atttggaaga
     2401 cctggacggc aaccgctatt tcgactttct gcaagctggt ggcccaacag tcctgggctc
     2461 aaacccaccc gaagtacgcg aaaaggtcat cgagttgcta aacacttgtg gtccctcaac
     2521 cggccttttt catgaatatg aatatcgcat tgccaaactg gttaccgagc ttattccatc
     2581 tgtcacctgg ctacgcatgt taaactcggg cacagaagcc tgcatggcag ctatccgtgt
     2641 tgccagactg gcgacaaagc acaggcac
//
LOCUS       DAOSFC010000199         2663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_36973_length_2663_cov_4.313267, whole genome shotgun sequence.
ACCESSION   DAOSFC010000199 DAOSFC010000000
VERSION     DAOSFC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36973_length_2663_cov_4.313267"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2663
                     /locus_tag="PK791_05415"
     CDS             <1..>2663
                     /locus_tag="PK791_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH92787.1"
                     /translation="ISHIIFKRYYEKHGKDLQTALFDTLEIELGSQRLKKTLLSYGEL
                     FPALEVYRGKIATIDYYRDETKGTPNAYLILEELILTWLLNQNPAAKPYQELFTDHFL
                     IKNTDYSQLISLVTEFFKEQPPFGADNLDLITFLRQPAILFPNSLNDQLEYISKHWTE
                     FIGERLSKLLSAIDHIAEESKAFFIHVPFRDEYAPTYLPDYSASFWADQKRAGDLENT
                     ENYSPDSDWMPKVVMIAKNSFVWLSQLSKQYQRNIQYLSDIPNETLDELAEWGFTALW
                     LIGLWERSNASQTIKKLCGNPDAVASAYSLDRYDIAQALGGYNAYKNLADRCAQRGIR
                     LASDMVPNHMGIDSDWVHDHPDWFIHLEHPPFPVYSFDGPELSSDPKVSIKLEDHYYS
                     RTDAAVVFKHYDHRNGQTRYIYHGNDGTSMPWNDTAQLNYLNPEVREAVIQTILHVAR
                     QFPIIRFDAAMTLTKKHFQRLWFPQPGTGGDIPSRAEHAMSKEDFDKAMPEEFWREVV
                     DRVATEVPDTLLLAEAFWLMEGYFVRSLGMHRVYNSAFMNMLRNEENAKYRQLIKSTL
                     TFDPEILKRYVNFMNNPDEKTAVEQFGKGDKYFGICTLLATMPGLPMFGHGQIEGYSE
                     KYGMEYYRPYWDEKPDQGFVDAHRHNIFPILHRRRLFAEVSNFRLYDFKINNDEIDEN
                     VFAYSNALGNSAALVIYNNHFGNAAGWIKTSAPYLVKHGNDNELHTENLAQALRLPDY
                     QNGFLVMRDQVSRKTYLRPMSEVHEKGLFFSLNAYERMVLSDFYVLYGEQYAQLHDYL
                     QGHGVDNLDATLEDLRLQPFLRKLDTLLHNLSNIAHAQSSNDKNALLEHAIDNWKELN
                     KATRSLDGFQVFESNNAFEDLILYLDTLRGL"
BASE COUNT          793 a          619 c          521 g          730 t
ORIGIN      
        1 atctcccata tcatcttcaa acggtattac gaaaaacatg gtaaagattt acagacggca
       61 ctgttcgata ccctggaaat cgaattaggt tctcaaagac tgaagaaaac attactcagc
      121 tatggagaac ttttccccgc attggaagta tatcgcggga aaatcgcaac gattgattat
      181 tatcgtgacg aaacaaaagg aacaccaaac gcttatctca tacttgaaga acttattctc
      241 acttggctac ttaatcaaaa tcctgctgct aaaccctacc aagaactgtt tactgaccat
      301 ttcttgataa aaaacactga ctattcacaa ctaatctctt tggtgacgga atttttcaaa
      361 gaacagccac cttttggtgc cgataatcta gacttaatca cattcttgcg ccaaccagca
      421 atcctttttc ctaactcact caatgatcag cttgaataca tttcaaaaca ctggaccgag
      481 ttcattggcg aacgtttgag taaattattg agtgccattg atcacattgc tgaagaatcc
      541 aaagcctttt tcatccatgt tcctttccga gatgaatacg ctcctactta cctgcctgat
      601 tattctgcat ctttctgggc agaccaaaaa cgtgctggcg atcttgagaa cactgaaaat
      661 tacagccctg acagtgattg gatgcctaag gtcgtgatga tagccaagaa tagttttgtc
      721 tggctaagtc agctttccaa gcaatatcaa agaaacattc aatatctaag cgatattcca
      781 aatgaaacac ttgatgagtt agccgaatgg gggttcaccg cgttgtggct tattggcctt
      841 tgggaacgga gtaatgcgtc ccaaactatc aaaaaacttt gtggaaatcc cgacgcggtc
      901 gcatcagctt attcgcttga ccgttacgat attgcccaag ccttaggcgg atataacgcc
      961 tacaaaaact tggcagaccg atgcgctcaa cgcggcataa ggctagcttc agatatggta
     1021 ccaaaccata tgggcatcga ttcagactgg gtccacgacc atcccgactg gttcattcac
     1081 cttgaacatc cccctttccc tgtttatagc tttgatggcc cggagctttc cagcgaccct
     1141 aaagttagca tcaaacttga agatcattat tattctcgaa ccgatgctgc tgttgtcttc
     1201 aaacattacg accatcgtaa tggtcaaaca cgatatatct atcatggtaa tgatggcacc
     1261 agtatgccct ggaacgacac cgcgcaacta aattatttga atccggaagt aagggaagca
     1321 gtcattcaaa ccattttaca tgtcgctcgt caattcccga ttatccgttt cgatgctgcc
     1381 atgacactca ccaaaaagca cttccagcgc ctttggttcc cgcagccagg gaccggcggc
     1441 gacattcctt cccgcgctga acacgccatg agtaaagaag attttgataa agccatgccc
     1501 gaagagtttt ggcgggaagt cgttgatcga gtggcgacag aagtgcccga taccttgctt
     1561 ctcgctgaag cattttggct gatggaaggc tactttgttc gttcgcttgg catgcaccgc
     1621 gtttacaaca gtgcctttat gaatatgctt cgtaatgaag aaaatgcgaa atatcgccaa
     1681 ctcattaaaa gcactcttac ctttgacccc gaaattctca agcgttatgt caacttcatg
     1741 aacaaccctg acgaaaaaac cgccgttgag caattcggga aaggtgataa atactttggc
     1801 atttgcacct tattggcaac gatgcctggt ttacccatgt ttggacatgg acaaattgaa
     1861 ggatatagcg aaaagtacgg catggaatac tatcgtccct actgggatga aaagcctgac
     1921 cagggctttg tagatgccca tcgacataac attttcccaa tccttcaccg gcgaaggctt
     1981 tttgcagagg tctccaactt cagactttat gatttcaaaa ttaacaatga tgagattgac
     2041 gaaaatgtct ttgcttacag taatgcactc ggaaattcag cagcccttgt gatatacaac
     2101 aatcattttg gaaacgcagc aggctggata aaaacatcag cgccttacct ggtaaagcat
     2161 ggcaatgata atgagttaca cacagagaac ctggctcaag cattacgatt gcccgactat
     2221 caaaatggct tcttagtcat gcgcgaccaa gtaagtcgca aaacctattt gcgtcctatg
     2281 tcagaagttc atgaaaaagg tcttttcttt tctctcaatg cctatgaacg tatggtttta
     2341 agcgactttt acgtattgta cggtgaacaa tatgctcaat tacacgacta tttgcaagga
     2401 catggggttg acaaccttga tgccacactt gaagatcttc gcttgcaacc attcttgaga
     2461 aaactcgaca ctttactcca taacttgtca aatatagctc acgctcaaag ttcaaatgat
     2521 aaaaatgcac tattagaaca cgcaatagat aactggaaag aattaaataa agctacccgt
     2581 tctcttgatg gattccaagt gtttgaatca aacaatgctt tcgaagatct tattctatac
     2641 ttagacactc tgcgtggatt gtt
//
LOCUS       DAOSFC010000200         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_37000_length_2662_cov_3.991178, whole genome shotgun sequence.
ACCESSION   DAOSFC010000200 DAOSFC010000000
VERSION     DAOSFC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37000_length_2662_cov_3.991178"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1485
                     /locus_tag="PK791_05420"
     CDS             325..1485
                     /locus_tag="PK791_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOH92788.1"
                     /translation="MIIGVPKEIMPGERRVAATPETVKAMRDMGLTVLVQKDAGEGAF
                     YHDPEYIAAGAEIVECPREIFERADMILKVKEPQFNKELDTHEVDLMHAGQYLITFIH
                     PAAPANHTMVKDMATKGIISITLDGIPRISRAQAMDALTSMSTCAGYKGSLLAANHLP
                     VFMPMMATAVGMIKPIQALVIGAGVGGLQAIATIKRLGAIVHAADIRPDAAEQAGSLG
                     AKVIDLGIDPKDAIGEGGYALALSPENLAKERAILAEVVKDMDIIFLSALVPGKLAPI
                     LITEEMVKTMKPGSVIVDISIDQGGNCEITPPGTTEQKHNVTIVGIKNIPGMLPTSST
                     WMFSKNVLHLVKYLIQDGKVVLDFDDQIIKEALTTYDGKVVHQGALEAFELD"
     gene            1555..1845
                     /locus_tag="PK791_05425"
     CDS             1555..1845
                     /locus_tag="PK791_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOH92789.1"
                     /translation="MSPLQIIMVVIFVVAAFVGYKIINRVPSMLHTPLMSGTNAFSGI
                     TITGCLSVTAIAIGLGSKILGLIAIVLAMINIVGGFGVTHRMLSMFQSKKDK"
     gene            1848..>2662
                     /locus_tag="PK791_05430"
     CDS             1848..>2662
                     /locus_tag="PK791_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOH92790.1"
                     /translation="MANTLLSMLSSTPIAETTDIIYYAIAAILTLAIVLGIAMLSKVK
                     TAVAGNLIGSAATLLAIVLAMWYFKIFSLIELWIAMLIGAVIGIIIAIRVKMIQMPQL
                     VALLNGFGGLASMITGILTVINDTQSDTFSMINAGIAIIIGGITFSGSMVAAGKLHKI
                     FPQKPIVLKGHQFWTALTLILSLVTIPLFVIPALQAQPYQTIVVIAASVFSLAFGVIF
                     SIRVGGADMPITISLLNSLTGVAAAGAGMAVNDILLVSVGGIVGASGLLLTQI"
BASE COUNT          665 a          686 c          574 g          737 t
ORIGIN      
        1 tgttggccat gtcggaaacg gccgcagagc taacattcat ataatcagcg attcgcttta
       61 ggctcgcata ttcggtttca tccagtgctt ccgcaatggc ttttatataa tcttctttac
      121 cgtggcttaa catcacatct ccatttagct atactttttc tttattttaa gcactactta
      181 agttttgtca agctgttttg cattcaagca aaaattgatt tcgtttcaat gcatagtttt
      241 caataatatt cctgggcaaa atctattcaa atgccctaat tcgtggtaaa tttaagctaa
      301 taacaaaagc aatggaggct ttgtatgatt attggtgtac caaaagaaat tatgccgggt
      361 gaacgccgtg tggctgccac tccggaaacc gtgaaagcca tgcgcgacat gggcttgacc
      421 gtgctcgtcc agaaagacgc gggtgaaggc gcattctatc acgacccaga atatatcgct
      481 gcgggagcag aaattgttga atgtccccgc gaaatctttg aacgcgcgga tatgatcctc
      541 aaagttaaag aaccgcaatt caacaaagaa ctggataccc acgaagttga tttaatgcac
      601 gctggacaat acctgattac cttcatccac ccagccgccc cagcaaacca caccatggtt
      661 aaagacatgg ctaccaaggg cattatcagc atcaccctcg acggcattcc acgcatttct
      721 cgcgcccagg caatggacgc cttaacatct atgagcacct gtgctggtta caaaggctcg
      781 cttttagccg ccaaccatct ccctgtcttc atgcccatga tggcaacagc cgttggcatg
      841 attaagccca tccaggcact cgtcatcggt gccggtgtag gcggtttgca agccatcgct
      901 accatcaaac gtcttggcgc aatcgttcac gccgcggata tccgccctga tgccgctgaa
      961 caggccggct cacttggcgc gaaagtgatt gacctgggta tagatcccaa agacgcaatc
     1021 ggcgaaggcg gttacgccct ggcactctcc cccgagaatc tcgccaaaga acgcgccatc
     1081 ctggctgaag ttgtcaagga tatggacatc atcttccttt ccgcgctcgt tcctggcaag
     1141 cttgccccca tcctgattac cgaagaaatg gtcaaaacca tgaaacctgg ttccgtaatt
     1201 gttgacatct ccatcgacca gggtggaaac tgcgaaatta cgcctcccgg tacaaccgaa
     1261 caaaaacaca atgtcaccat cgttggcatc aagaacatcc ctggcatgct gcccaccagc
     1321 tccacctgga tgttctcaaa gaacgttttg catctggtga agtatctcat ccaggatggc
     1381 aaagttgtgc tcgatttcga cgaccaaatc atcaaggaag ccttaaccac ctatgatggc
     1441 aaggttgtcc accaaggcgc tctcgaagcg tttgagcttg attaagaata caaaaactat
     1501 cacccatgtg ggggcttata aaagccccca catttttgag tttaaggagg attagtgagt
     1561 ccattacaaa tcattatggt cgtcatcttt gtagtcgcgg ctttcgtcgg ctacaagatt
     1621 attaaccgcg tacccagtat gcttcatacc ccattgatgt cgggtacgaa cgcattttcg
     1681 ggcattacca ttaccggttg tcttagcgtt acagctatag ccattggcct tggcagcaaa
     1741 atacttggtt taattgccat cgtattggct atgatcaata tcgtcggcgg ttttggtgtg
     1801 acccaccgca tgctctccat gttccagagt aagaaggata aataactatg gctaacacac
     1861 tactaagcat gctttcatcc acccctatag ctgaaactac agacattatc tattacgcta
     1921 ttgccgccat tcttaccctt gcaattgtgc tgggaatcgc catgctaagc aaagttaaaa
     1981 ctgcggttgc tggcaacctg attggcagtg ctgccacact ccttgctatc gttctcgcca
     2041 tgtggtattt caagatcttc tcattgattg agctttggat tgccatgttg attggtgcag
     2101 tcatcggtat catcattgcc attagagtta agatgattca aatgccacaa cttgtggctt
     2161 tgttgaatgg ttttggtggt cttgcttcaa tgattacggg tattctcacc gtcatcaacg
     2221 acacacaatc cgatactttc tctatgatta atgctggcat agcgattatc attggtggta
     2281 ttaccttctc cggcagcatg gtagcagcgg gtaaactcca caaaatattc ccgcaaaaac
     2341 cgatcgttct caaaggccat caattctgga cagccctcac cctgatcctc tcgctcgtta
     2401 ccataccatt gtttgttatt ccagcactgc aagcacaacc ctatcaaacg attgtcgtta
     2461 ttgctgcaag cgtctttagt cttgcctttg gcgtgatatt ctctattcgc gttggtggcg
     2521 cggatatgcc tatcacgatt tccctgctca actcgctgac tggtgttgcc gccgcaggcg
     2581 cgggtatggc agttaacgat atccttttgg tctcagttgg tggtatcgtc ggtgcatctg
     2641 gtttgctttt gacccaaatt at
//
LOCUS       DAOSFC010000201         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_37037_length_2660_cov_6.234165, whole genome shotgun sequence.
ACCESSION   DAOSFC010000201 DAOSFC010000000
VERSION     DAOSFC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37037_length_2660_cov_6.234165"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PK791_05435"
     CDS             <1..1038
                     /locus_tag="PK791_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH92791.1"
                     /translation="PRIGGVLIRGERGTAKSTAARALAALLPEIEVVKGCRFACDPAH
                     PSRFCTECLERLEAGEDPLPTETRKTPFINLPVSATEDRVVGTLDIERAIQLGERHFE
                     PGVLAAANRGMLYIDEVNLLDDHVVDILLDSAAMGVNVVEREGISFSHPARFILVGTM
                     NPEEGDLRPQLLDRFAFSVDIHGILDKAKRVEIMRRNIAFEADPAAFQAKWAPKEQEL
                     SNKIKAAQYLLDEVSYTQRDLTLIAELTSSMEVDGHRSDMVILKGARAQAAFDGRRRI
                     SPVDIGIAAELALPHRVKAGPFRKSGVGIETLNEKIDGLKALSTVTEYPEEDGDDGAT
                     PDDSDKKKATL"
     gene            1149..2054
                     /locus_tag="PK791_05440"
     CDS             1149..2054
                     /locus_tag="PK791_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH92792.1"
                     /translation="MEASETFEPRQLDTPLDEMVRKQGGRRSQTRSKTKRGRYIAARH
                     APGDTSDLAFDATLRAAAPYQRKRQALKERNKVAFAVRSADFQKKIRMRKTANLILFV
                     VDGSWSMAVSERMSATKAAILSLLTDAYQRRDRVGLVVFQKDRATLVLSPTNSVVLAQ
                     RAMENIPIGGKTPLAAGLVMAYEVVQREKILHPDVQPLLIVLTDGAGNVAYQPGRNVP
                     QELEELAGMFKREKIHSVVINMEQKVFDAGLAQRLADNMGAVCYTVDELRGDILYETV
                     QKEIIAPSGPNYRDTAPVMATSRDW"
     gene            complement(2066..2542)
                     /locus_tag="PK791_05445"
     CDS             complement(2066..2542)
                     /locus_tag="PK791_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HOH92793.1"
                     /translation="MKSLYIQQKFFKILDHYPITDENGQTIYQVDQEFNPIGHTVHVS
                     DPNGNHLFTVDRVVLTLLPKFMVRFANGKEISIQSNFSLLHRDIDIDPEHLGLNVMGD
                     FWGHEYTLTQRGNTVGSISKEWLSLTDNYRLDIFDETLQDLFVAVVIAIDCLLDES"
BASE COUNT          616 a          707 c          773 g          564 t
ORIGIN      
        1 ccgcgcatcg gcggcgtgct gattcgtggc gaacgtggca ccgccaaatc gactgcggcg
       61 cgcgccctgg cagccctgct gccggaaatc gaagtggtga aaggctgtcg ctttgcctgt
      121 gacccagccc atccaagccg cttctgcacg gagtgccttg agcgccttga agccggcgag
      181 gaccccctgc ccaccgaaac ccgcaagaca cccttcatca acctgccggt ttctgccacc
      241 gaagaccgtg tggtgggcac gctggatatc gaacgcgcca tccagctggg tgagcgtcat
      301 tttgagcccg gcgtgctggc agcggcgaac cgtggcatgc tttatatcga cgaagtcaac
      361 ctgctggatg accatgtggt ggatatcctg ctggattccg ccgccatggg cgtgaacgtg
      421 gttgagcgcg agggcatttc gtttagccat ccggcgcgct ttatcctggt gggcacgatg
      481 aaccccgaag aaggcgattt gcgtccgcag cttttggacc gctttgcttt ttcggtggat
      541 atccatggca ttcttgacaa agccaaacgg gttgaaatta tgcgccgaaa tatcgccttt
      601 gaagccgacc ccgccgcttt tcaggcgaag tgggcaccca aagagcagga actgagtaat
      661 aaaatcaaag ccgcgcagta cctgctggat gaggttagct acactcagcg cgacctgacc
      721 ttaatcgcgg agctgacgtc atcaatggaa gttgacgggc accgctcgga tatggtcatc
      781 cttaaaggcg cgcgcgccca ggcggcgttt gacgggcgca gacgcatctc gccggtggat
      841 attggcatcg cggctgagtt ggcgttgccc catcgcgtta aggcgggtcc attccgcaag
      901 agtggtgtgg gcattgaaac gctcaatgag aagattgacg gactaaaagc gcttagcaca
      961 gtgacagaat atcccgaaga agatggcgac gacggcgcca cgccggacga ttccgataaa
     1021 aaaaaagcga ccctctagct gagggcgaag ttcagcctga tatttcagat gaaatcccgc
     1081 tggtagggga aggccagcag gatattttca ccacgcttga cgcgcactgg tgggaaggcg
     1141 ggcaggtgat ggaggccagc gagacctttg aaccgcgcca gcttgacacg cccctggacg
     1201 aaatggtgcg caaacagggt ggcagacgct cgcaaacccg ctcgaaaacc aagcggggtc
     1261 gctatattgc cgcgcgccat gccccgggcg acaccagcga ccttgccttt gacgccactc
     1321 tgcgcgctgc cgcgccttat cagcgcaaac gccaggcgct aaaagagcgc aacaaggtgg
     1381 ctttcgcggt gcgttcggca gatttccaaa agaaaattcg catgcgcaaa acagccaacc
     1441 tgattctctt tgtggtggat ggctcgtggt cgatggcggt atcagagcgc atgtcggcaa
     1501 ccaaagcggc gattttgtcc ctgcttaccg atgcttatca acgacgcgac cgggtaggtc
     1561 tggtcgtttt ccaaaaagac cgcgcgaccc tggtgctttc gcccaccaac tcggtggttt
     1621 tggcccagcg cgctatggaa aacatcccca tcggtggcaa gacgcccctc gcggcgggtt
     1681 tggtgatggc ttatgaggtg gtgcagcgcg agaagattct gcaccccgac gtgcaacccc
     1741 tgttaatcgt actcaccgat ggcgccggca atgtggccta tcaacccggg cgcaatgtgc
     1801 cccaggagct tgaggaactg gcgggcatgt tcaagcgcga gaagattcac agcgtggtga
     1861 ttaacatgga gcaaaaggtc tttgacgccg gcctggcgca acgcctggcg gataatatgg
     1921 gcgcggtttg ctataccgtg gacgaactgc gcggggatat cctttacgaa accgtgcaaa
     1981 aagagatcat cgcgccgagt gggcctaatt atcgcgatac cgcgccagtg atggcaacca
     2041 gccgtgactg gtagggcttg gaccgtcagg attcgtcaag taagcagtca atggcaatga
     2101 ccacggcaac aaagagatcc tgcaaagtct cgtcaaaaat atccagtcga taattgtcag
     2161 tgagagaaag ccattctttg gaaatgctgc caacggtgtt tccgcgttgg gtaagggtat
     2221 attcgtgccc ccaaaagtca cccatcacat taagccctaa atgttccgga tcgatgtcaa
     2281 tatcccggtg aagtaagctg aagttacttt ggatgctgat ttccttcccg ttggcaaacc
     2341 tgaccatgaa ttttggcagt aaggttagta caaccctgtc cacggtaaaa aggtgattcc
     2401 cgttggggtc tgaaacatga acggtatggc cgatagggtt gaactcctgg tcaacctgat
     2461 agatggtttg tccgttctca tcggtgatgg gatagtggtc caggattttg aaaaattttt
     2521 gctggatata aagtgatttc atgcttcgcg acctcatttc ctgatgtttt taaagtttag
     2581 cataactcga aaaagcactg cgacaaggca ttaattaaaa cacggtgcca tcatcagagt
     2641 tgcaaacggg caatcggctg
//
LOCUS       DAOSFC010000202         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_37093_length_2657_cov_6.612221, whole genome shotgun sequence.
ACCESSION   DAOSFC010000202 DAOSFC010000000
VERSION     DAOSFC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37093_length_2657_cov_6.612221"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PK791_05450"
     CDS             <1..762
                     /locus_tag="PK791_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH92794.1"
                     /translation="DALPSALRALEPEVIIHCAAIANVDVAEKQPELSQRINADAPRV
                     IASVAKQLGAKMIQISTDAVFDGSKGNYKEDDAVNPLNAYARTKLAGERAVIQANPDA
                     AIARVVFYGWTLEGNRSLAEFFYNNLVAGKSVNGFTDAYFNPLYVRDLAETLLEIAEA
                     NLKGIWHTFGNDTLSKYDFGVALAREFGLDESLIKPVSASQGRDVVRSLNLSTNSDKL
                     ARALGHPLPGLTQGLTRLKEDFDAGWRERLAKFKA"
     gene            785..1849
                     /locus_tag="PK791_05455"
     CDS             785..1849
                     /locus_tag="PK791_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOH92795.1"
                     /translation="MELKIGNTIIGNNHPTYFVADIAANHDGSLERAIELIRLAAEAG
                     ANAAKFQNFRGPEIVSDYGFSHMDGQVSHQAKWKKSVTEVYNAASIPFEWTPILREAC
                     DKYGIEFFSAPYDFDAIDMLEEHVPAYKIGSGDITWIEACLRIAKKGKPVLLATGASS
                     IGDVQRAVDAILEVNPQLVLMQCNTNYTAEDGNFDNIDLNVLKTYHKMWPQLILGLSD
                     HTHGHATTLGAVALGARVVEKHFTDDNHREGPDHAFAMNPQSWAEMVRNTRQLERALG
                     TGNKRVTANEQETVVVQRRCLRAARDIKAGETVTRDMVAVLRPATPGAIEPYDIDAVI
                     GKRARQNLEHGQDLRWTHLE"
     gene            1852..>2657
                     /locus_tag="PK791_05460"
     CDS             1852..>2657
                     /locus_tag="PK791_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH92796.1"
                     /translation="MRILYFTRADSVHDQRFMAALAGSGHEAYVLRLYEGDYKTPEGV
                     SAIAFKGIKGPLRLLQIPGLVARLKKLLEKLKPDLVHAGPLHDLAYLIAKTGYKPLLS
                     MSWGFDLMRDVNVDAGMASRARFALANSRALITDARCSAQKAVELGFDENRICIFPWG
                     VDIDKFSPQAVVTAGKRWREAQNWQDNTVLLCLRAMEANYGVDVLAKAFGIAAKNDPD
                     LRLLLLGDGSQRSNIEKIFDEAGVSEQVWFGGRVPNDTLGTYYGAADIYVT"
BASE COUNT          646 a          693 c          714 g          604 t
ORIGIN      
        1 gacgctttgc cttccgcatt gcgggcactc gagccggaag tgattatcca ctgcgcggcg
       61 attgctaacg ttgatgtcgc ggaaaaacaa cccgaactca gccagcgcat caatgctgat
      121 gcgccccgtg tcatcgccag tgtagccaaa caacttggcg cgaagatgat tcaaatctcc
      181 accgacgcgg tttttgatgg cagtaaaggc aactataagg aagacgacgc ggtcaacccg
      241 ctcaacgcct atgccaggac aaaactcgcc ggtgagcgcg cggtgataca agccaacccc
      301 gatgcggcca tcgcccgcgt ggtcttttat ggctggactt tggaaggcaa ccgcagtctg
      361 gcagaatttt tctataacaa tctcgttgcc ggcaaatccg taaatggttt tacggatgct
      421 tacttcaatc ccttgtacgt gcgcgatttg gcagaaacat tgcttgaaat cgccgaagcg
      481 aatctcaagg gcatctggca cacttttggc aacgatacgc ttagcaaata tgattttggc
      541 gtcgctttgg cgcgcgagtt tgggctggac gaaagcttaa tcaagcctgt atcggcatcg
      601 cagggtcgtg acgtggttcg ttcactcaac ctgagcacga acagcgataa attagccagg
      661 gctttggggc atcccttgcc cggattgacg cagggactta ccagactcaa agaagatttc
      721 gacgcgggct ggcgtgaaag actggcaaag ttcaaagcct gagacccgca aaaacggagg
      781 catcatggaa ttgaaaatcg gaaacacaat catcggtaac aatcatccca cctatttcgt
      841 ggccgacatc gccgccaacc acgatggttc actggaacgc gcgattgaac ttattcgcct
      901 ggcggctgaa gctggcgcca atgctgccaa gtttcaaaac tttcgtggac ctgaaatcgt
      961 ctctgattat ggcttttctc acatggatgg tcaggtatcg catcaggcaa agtggaaaaa
     1021 gagtgtcact gaagtttata acgcagcttc cattcctttt gagtggacgc ccattctcag
     1081 agaagcttgc gacaaatacg gtattgagtt tttctccgcg ccctatgact ttgacgccat
     1141 tgatatgctg gaagaacacg tacccgccta caaaatcggc tcgggcgata ttacctggat
     1201 tgaagcctgc ctgcgcatcg ctaaaaaagg caaacccgtc cttttagcga caggcgcgtc
     1261 cagtatcggc gacgtgcaac gcgcggtgga tgccattttg gaagtcaatc cgcagttggt
     1321 cctgatgcaa tgtaatacca actacaccgc cgaagacggt aattttgaca acatcgacct
     1381 gaatgtgctt aagacctacc ataaaatgtg gccacaattg attttaggtt taagcgacca
     1441 cacccacgga cacgccacga ccctgggcgc ggtggcactc ggcgcgcggg ttgttgaaaa
     1501 acactttacc gacgacaacc accgcgaagg tcctgaccat gctttcgcca tgaaccccca
     1561 aagctgggca gagatggtac gtaacacccg ccagcttgag cgcgccctgg gcaccggtaa
     1621 caaacgcgtt accgcgaacg aacaggaaac cgtggtggta caacgacgtt gcttgcgagc
     1681 cgcgcgcgat attaaggcag gcgagacggt tacccgcgat atggttgccg ttttacgccc
     1741 ggctacgcct ggcgcgattg agccttacga cattgacgct gtaattggca aacgcgcgcg
     1801 tcaaaacctt gaacacggtc aggatttgcg ctggacacat ctggagtaaa gatgcggatt
     1861 ttgtatttca cgcgcgcgga tagtgtgcat gaccaacgct ttatggccgc gctggctgga
     1921 agtggtcatg aggcttatgt tttgcgtctc tatgaagggg actataagac ccctgaaggt
     1981 gtgagcgcaa tcgctttcaa agggattaag ggccccttgc gcttgctgca aatccctggc
     2041 ctggtcgcgc gtctgaaaaa acttctggaa aagctaaaac ccgacctggt gcatgccgga
     2101 cccttgcatg atttggctta tctcatcgcg aaaacgggtt acaagccctt gctgagcatg
     2161 agctggggct ttgacttgat gcgggatgtg aatgtcgatg ccggtatggc cagccgggcg
     2221 cgttttgccc tggcaaactc cagagccttg attaccgacg cgcgttgttc agcacaaaaa
     2281 gctgttgaac tcggtttcga cgaaaaccgc atttgcatct tcccctgggg cgtggatatt
     2341 gataaattct cacctcaggc ggtcgtaacc gccggcaaac gctggcgcga agcccaaaac
     2401 tggcaggaca ataccgtgct tttgtgcttg cgtgctatgg aagcaaatta tggcgtggac
     2461 gtactggcaa aagcttttgg catcgccgct aagaatgacc ccgacctgcg ccttttgctt
     2521 ttgggagatg gttcccaacg gtcaaatatc gaaaagattt ttgatgaagc tggcgtgagc
     2581 gaacaagtct ggtttggtgg tcgtgtgcct aatgacacct tgggcacgta ctacggtgca
     2641 gccgatatct acgtcac
//
LOCUS       DAOSFC010000203         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_37279_length_2648_cov_4.533359, whole genome shotgun sequence.
ACCESSION   DAOSFC010000203 DAOSFC010000000
VERSION     DAOSFC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37279_length_2648_cov_4.533359"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK791_05465"
     CDS             complement(<1..312)
                     /locus_tag="PK791_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOH92797.1"
                     /translation="MEQTIKDLKEAFAGESQAYQKYVAYAQKAERDGFPGIAKLFQAT
                     AQAERIHAAGHLRALSMIKDTLENLEDAIAGETYEYKEMYPRMLENAEKIDHRAKVMF
                     GY"
     gene            512..584
                     /locus_tag="PK791_05470"
     tRNA            512..584
                     /locus_tag="PK791_05470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:545..547,aa:Gly,seq:gcc)
     gene            628..698
                     /locus_tag="PK791_05475"
     tRNA            628..698
                     /locus_tag="PK791_05475"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:660..662,aa:Cys,seq:gca)
     gene            complement(747..1556)
                     /locus_tag="PK791_05480"
     CDS             complement(747..1556)
                     /locus_tag="PK791_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOH92798.1"
                     /translation="MNKESEKKNIKALMLDMDGTFYMGDVLLPGALELMSFLNEREIP
                     FSFLTNNSSKSADDYVKKLIGLGVKPEDARVYTSGDATFDYVLREHAGKKVFLMGTQS
                     LRESFEKAGICLVEEDPDLVVLGYDTELTYPVLTRFCDFVRAGLPYLATHPDVNCPVL
                     GGYAPDIGAMMAMIEASTGRKADLILGKPNPGIVEGLAFKWGLRNDEILMVGDRLYTD
                     IMMGKTAGVKTALVLTGEASREDLQSAEHQPDWVVEDLFALIHKLKIAKSC"
     gene            1633..1836
                     /locus_tag="PK791_05485"
     CDS             1633..1836
                     /locus_tag="PK791_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOH92799.1"
                     /translation="MKTLSYSIPNINCNHCVNTIQAAIEQMPGVDFVEADPNSKTLTI
                     EGSNDLDDEIIRAKLIEIGFPAK"
     gene            1850..>2648
                     /locus_tag="PK791_05490"
     CDS             1850..>2648
                     /locus_tag="PK791_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOH92800.1"
                     /translation="MSTIKQITLPILGMTCANCVAAVEKTLDQQNGIEKVLVNLSSER
                     AFVSYDLEHINLNDIIATIEGAGYKVATGNINMTLAGLNDPANQRAIEKRMAESFGII
                     ESHANIASERLSLIYIPTIISPTEIREIIIKAGFKIVSDSSLTDNEDAETQARKLAIK
                     NQRHMLIFALIFTVPLFILHMLHSFQLLPSAVMTAPWLEWLFFALATPVQFVAGWSFY
                     INGYKALKNGAANMDVLVAMGSTTAYIYSILIMLGIFPGHGYFESAAV"
BASE COUNT          736 a          661 c          513 g          738 t
ORIGIN      
        1 gtagccaaac atcactttgg cacgatggtc gattttttca gcgttctcca gcattcgcgg
       61 atacatttcc ttgtactcgt aggtctcgcc agcgatagcg tcttcgagat tctcaagcgt
      121 gtctttgatc atactcaaag cgcgcaagtg acccgcggcg tgaatacgtt cagcctgagc
      181 agtagcctgg aaaagtttcg cgattccagg aaaaccatcg cgttcagcct tttgtgcgta
      241 ggcgacgtac ttttggtatg cctggctctc tccagcaaat gcctctttta agtctttgat
      301 tgtttgttcc attgttcctc cgaaagtaaa ttttggttac aggctaattt tacctgaaag
      361 ttatgagaat ctgcttaatc atgtaaaacc atcagtctca gcgatatgta aacttccttt
      421 cacacaaaat gtgctaaaaa atttgacaag tccagtttca tgtgctaaaa tcttgtcctg
      481 ttcggtacgg aaccgcaaca aacatttgac cgcgggtgta gttcaattgg tagaacgtct
      541 ccttgccaag gagaaggtcg tgagttcgag cctcatcgcc cgctctttac cagactgata
      601 gcggtaacgg tatcagtctt tttattaggc gacgtggcca agtggtaagg caggagtctg
      661 caaaactctc atcatgggtt cgattcccat cgtcgcctct tttttgtgtc tgtttttagc
      721 tcaagtacgt tgtttagaat attgaattag caacttttag caattttcaa tttgtgaatt
      781 aaagcaaaca aatcctcaac cacccaatcc ggttgatgtt ccgcgctttg caaatcctct
      841 cgcgatgcct cgccagttaa gaccagcgcg gtctttacac cagctgtttt gcccatcata
      901 atatccgtat aaagtcggtc gccaaccatc agaatttcat cgtttcgcaa tccccacttg
      961 aatgccaatc cttccacaat ccccggattt ggcttgccta atatcaaatc agccttacgg
     1021 ccggtggaag cctcaatcat agccatcatt gccccaatat ccggcgcgta cccgcccaaa
     1081 acaggacagt ttacatctgg atgcgttgca agataaggta aacccgcgcg gacaaaatca
     1141 caaaaccggg tcaataccgg gtaagtcaat tcggtatcgt aacccagcac gactaaatca
     1201 gggtcttctt caaccagaca aatacctgcc ttctcaaagc tctcacgcaa gctttgagta
     1261 cccatcaaaa aaaccttctt accagcatgt tcacgcaaga cataatcaaa agtcgcgtca
     1321 cccgaagtat agacccgcgc atcttcaggt ttcacgccca gtccgattag ttttttaaca
     1381 taatcgtcgg cacttttact gctgttgttg gtaagaaatg aaaacggtat ctcccgctca
     1441 ttcaaaaaag acatcagttc caaagcaccc ggcaacaaga catcccccat ataaaacgtg
     1501 ccatccatat ccaacatcaa ggccttaata tttttctttt cactttcttt attcatttct
     1561 tttcctcctg ttgcaatcat caatataatt gactacaata ttgttagtct tgaataacaa
     1621 atgaggtgaa acatgaaaac acttagctat tctatcccaa atattaattg taatcactgt
     1681 gttaatacca ttcaggcagc aattgaacaa atgcctggtg tggattttgt agaagctgac
     1741 ccaaacagca aaactttaac cattgaaggc tcgaacgact tggatgacga aatcattcgc
     1801 gctaaactga ttgaaatcgg ttttcctgca aaataaagca ggctgactta tgtcaaccat
     1861 aaagcaaata accctgccta ttctggggat gacctgtgcc aactgtgttg ccgcagttga
     1921 aaaaacgctt gatcaacaaa acggcattga aaaagtcctt gtcaatctca gctccgaacg
     1981 cgcctttgtc agttatgacc tggaacacat caacctgaac gatattatcg ccacgataga
     2041 aggtgcaggc tataaagtcg caactggcaa tataaatatg actttagctg gactcaatga
     2101 ccccgccaac caacgcgcta tcgaaaaacg tatggcggaa agttttggca ttatcgaaag
     2161 ccatgccaat attgccagcg agcgcctttc gttgatctac attccaacca ttatcagccc
     2221 aacagaaatt cgtgaaatta tcatcaaagc tggtttcaaa atcgtctctg attcaagctt
     2281 gacagataac gaagatgccg aaacccaagc ccgcaaactt gccatcaaaa atcaacggca
     2341 tatgctcatc tttgcgttaa tattcacagt gccacttttt atcctgcata tgctgcatag
     2401 ttttcagctt ctgccatcag cggtgatgac cgcgccctgg ctggagtggc tcttttttgc
     2461 gcttgcaaca cccgtacaat tcgttgccgg ctggagtttt tatattaacg gctataaagc
     2521 actcaaaaat ggtgccgcca atatggacgt gctcgttgcg atgggtagca ccaccgccta
     2581 tatctactcg atactcatca tgctgggtat atttcctgga catggttatt ttgagagtgc
     2641 cgctgtca
//
LOCUS       DAOSFC010000204         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_37928_length_2618_cov_5.688646, whole genome shotgun sequence.
ACCESSION   DAOSFC010000204 DAOSFC010000000
VERSION     DAOSFC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37928_length_2618_cov_5.688646"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PK791_05495"
     CDS             <1..525
                     /locus_tag="PK791_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOH92801.1"
                     /translation="KEKWLDAMPPFLSGGEMISNVTPEKISFNVLPYKFEAGTPNVPG
                     PIGLAAAIDYLQEFDEQELRAHEDALLNYGIEKLKEIPGLTLYGNPKQRSSILPFNIE
                     GIQHFDLGVLFDARGVAVRTGQHCTQPIMDALKVPGFVRASLALYSNREDIDALVESI
                     QRAIKMLKGAKKHG"
     gene            518..928
                     /locus_tag="PK791_05500"
     CDS             518..928
                     /locus_tag="PK791_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOH92802.1"
                     /translation="MDKRRAQLMEDFDLLENWEEKYDYLIELGFDLDEMPQDLKTEEN
                     LVKGCQSSVWFDIRCENGKIYFAADSDSLIVKGIVAIVDYLYNGQSAEDVLKADLSIF
                     EELGLWQHLSSQRGNGLMAMLAHLKKAATDCLSQ"
     gene            982..1533
                     /locus_tag="PK791_05505"
     CDS             982..1533
                     /locus_tag="PK791_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH92803.1"
                     /translation="MKASKVLVFILCVAFVLGVFLWQTSLPVSGASNELPMAQIITST
                     PLPDGSIYHIPVEGDTLWAISEAYGVSMRDINILNGNSPEANEIYIGQRILIRRGEPM
                     TATPEASPTAMPVTPSPTVFKPSRTPIPSATAIPSPTATQPPSRVQVIFGDSQRVGWT
                     ITTISLVGIALVVFFGFIYKPKP"
     gene            1559..2122
                     /locus_tag="PK791_05510"
     CDS             1559..2122
                     /locus_tag="PK791_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOH92804.1"
                     /translation="MSNNKTPVHAIAFDFVGVLFDFRAGDYTPRQLAIASDFGRFVTD
                     EEHIAYYSKKYATPPDQIVEELRYICSHAYKIREPDILEKIPCFKFAAASNHLSFAMD
                     WFRTQPISEQFEVFFASGSLGLAKPDPRFFRALCEALDEKPENVLFVDDTLRHILAAK
                     DCGLQALHFDSKKVLSEEINRYLADHD"
     gene            2115..>2618
                     /locus_tag="PK791_05515"
     CDS             2115..>2618
                     /locus_tag="PK791_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH92805.1"
                     /translation="MIEDVKIVELDKSWKERVFVYRYTTSFYYDLELMDKADGNFCFC
                     LTRKAFERPVEKQFEGSLLSDWLFEPVAYGAFDGKTLLGVMCISVEDWNNRLRVAELW
                     VDEPFRHQGVGKKLMAKAIDYARSKNLRGLVLETQSCNEPAIRFYQSCGLRFIGLDAT
                     HYSNDDIL"
BASE COUNT          670 a          608 c          638 g          702 t
ORIGIN      
        1 aaagaaaaat ggcttgatgc catgcctcct tttctctcag gtggcgagat gatcagtaac
       61 gtaacacctg aaaagattag ctttaacgtt ttgccttaca aattcgaagc tggtacgcca
      121 aacgtgcctg gtccgattgg tcttgcagcc gcgattgact atcttcaaga atttgatgag
      181 caggaattgc gcgcccatga ggatgctttg ttgaactatg gcatcgaaaa actgaaagaa
      241 atccccggct tgacgctata tggcaacccc aaacagcgca gcagcatttt gcctttcaac
      301 attgaaggta tccagcattt cgatttgggt gttttatttg acgcgcgcgg tgtcgcggtg
      361 cgtaccgggc agcattgcac ccaaccgata atggacgcgc tgaaggtccc gggctttgtg
      421 cgtgcatcgc tggcgcttta cagcaatcgc gaagatattg acgcgctggt ggagagcatc
      481 caaagggcta taaaaatgct aaaaggagca aaaaagcatg gataaacgca gagcgcaatt
      541 gatggaagac tttgatctgc ttgaaaactg ggaggaaaaa tacgattatc tcatcgagct
      601 tggtttcgac ctggatgaga tgccccagga tttaaaaacc gaagaaaacc tggtaaaagg
      661 ctgtcagagc agtgtgtggt ttgatatccg ctgtgagaat ggcaaaattt attttgccgc
      721 ggacagtgac tccctgattg tcaaaggcat cgtagcaatt gttgattatc tttacaacgg
      781 ccaatctgca gaagatgtgc ttaaagccga tttgagcatc tttgaggaac ttggtctttg
      841 gcagcatctt tcttcgcaac gcgggaacgg attaatggcc atgcttgcgc atcttaaaaa
      901 agccgcaacg gattgcttaa gccagtagca catttggggc agaaaaacgc aggaacatta
      961 aatcggttta taatatcaat catgaaagca agtaaagttt tagttttcat cttgtgtgtg
     1021 gcgtttgtgc ttggcgtttt cctttggcaa accagtttgc cagtgagtgg cgcatcaaat
     1081 gaactcccta tggcacagat tatcacatca acccccttgc cagatgggtc tatttatcat
     1141 atccctgttg aaggcgatac gctttgggca atctctgaag cttacggcgt ttcgatgcgg
     1201 gatatcaata ttctcaatgg caacagtccc gaggcgaacg agatttatat cggtcagcgt
     1261 atacttatca gacgtggcga gccgatgacg gctacccccg aagcctcacc gaccgcgatg
     1321 ccggtcacac cgtcgccaac tgtcttcaaa ccgtcgcgca cacctatccc aagtgccacc
     1381 gcaatcccca gcccaactgc cacccagcca cccagcaggg ttcaagttat ttttggcgac
     1441 agtcaacgtg tgggttggac catcacgaca atcagcctgg tgggcatcgc gctggttgtt
     1501 ttctttggct ttatctataa gcccaaaccg taattctgat ttacactttt ggtgaactat
     1561 gagtaataat aaaacccctg tgcatgccat tgcgtttgat tttgtgggcg tgctttttga
     1621 ttttcgtgct ggtgattaca caccccgtca actggccatc gcgagcgatt tcgggcgctt
     1681 tgtcaccgat gaagagcaca tcgcgtacta ttcaaaaaaa tatgccactc cacccgatca
     1741 gattgtggaa gaattgcgct atatttgcag tcacgcttat aaaatacgcg aaccggatat
     1801 tttggagaaa attccctgtt tcaaattcgc cgcagccagc aatcaccttt cctttgccat
     1861 ggactggttc cgcacacaac ccatttcgga gcagttcgaa gtctttttcg cttctggttc
     1921 actcggtttg gcaaaacccg acccgcgctt tttccgtgct ttgtgtgaag ccctggacga
     1981 gaagcccgaa aatgtactct ttgtcgatga caccttgcga catatcctgg cggctaaaga
     2041 ctgtggcttg caagccttgc atttcgattc caagaaggta ctttcagaag aaataaaccg
     2101 atatttggca gaccatgatt gaagacgtca aaatcgttga acttgataaa agctggaaag
     2161 aacgggtgtt tgtgtatcgc tacaccacgt ctttttatta tgatcttgag cttatggaca
     2221 aggcggatgg caacttttgt ttttgtttga caaggaaagc ctttgaaagg cccgtcgaaa
     2281 aacagtttga aggctcactt ctctctgact ggttgtttga gcctgtggca tacggcgcgt
     2341 ttgacgggaa gactctgctt ggcgttatgt gtattagcgt ggaagactgg aacaaccgtc
     2401 tgcgcgtagc cgagttgtgg gttgatgaac ccttccgtca tcagggcgtg ggaaaaaagc
     2461 tgatggcaaa agctatcgat tacgcgcgtt caaaaaacct gcggggtctt gtgctggaga
     2521 cgcaaagctg taacgaaccc gcaatccgtt tctatcaatc ctgtggttta cgctttatcg
     2581 ggcttgatgc cacccactat tcaaatgatg atattctc
//
LOCUS       DAOSFC010000205         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_38017_length_2615_cov_5.498047, whole genome shotgun sequence.
ACCESSION   DAOSFC010000205 DAOSFC010000000
VERSION     DAOSFC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38017_length_2615_cov_5.498047"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..420)
                     /locus_tag="PK791_05520"
     tRNA            complement(348..420)
                     /locus_tag="PK791_05520"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Phe,seq:gaa)
     gene            742..1746
                     /locus_tag="PK791_05525"
     CDS             742..1746
                     /locus_tag="PK791_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH92806.1"
                     /translation="MARVTIRDVAKEAGVSTTTVSHVINETRFVEEKTRAKVNEAIAT
                     LGYRVNIAARSLRAGRSQTIGLIIPDAANLFFAEYLRKIEDFGFKAGYNVIVCNTDNE
                     TDKEAAYIDALVSKQVDGVILISSGSDPETLNPLISHDVPFVIADREFSADVADVVSL
                     DNEQAGFDVTNYLLNLGHRKIGCITGPNNFSPSIQRLNGFIRAHKEWGIPYDPELITS
                     GKFTLSSGFSAFDWIWQNNSKPSALFVFSDMMAIGAINNAITKGLNVPKDISIVGFDN
                     IELAEAFAPSITTFNQPIEKMAETIVELLTDRIKHKSANRRRIVFRGSLIIRESTTKY
                     "
     gene            1754..>2615
                     /gene="rbsK"
                     /locus_tag="PK791_05530"
     CDS             1754..>2615
                     /gene="rbsK"
                     /locus_tag="PK791_05530"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOH92807.1"
                     /translation="MNRISVIGSLNMDLVIQTERVPLAGETISGRDFNLIPGGKGANQ
                     AIAAAKAGSPTSMFGCVGDDEFGEVLLNSLKDAGVNTVNVSKTSKVRTGVASIILESS
                     GENRIIIISGANNLVDAAYVDSHWDEIKLSSLILLQHEIPLETCHYIIEKAHASSIPV
                     MLNPAPFHEIPTDVLTKVDYLVVNEVELQGIVPAQVSSIEDVIRAGKKINQMGVKQVL
                     ITLGGDGAVLVSNEEVIHQPAFTVNAVDTTAAGDTFVGYFASAIINGFGLKQALVNSC
                     GAAALAVTKVG"
BASE COUNT          752 a          606 c          587 g          670 t
ORIGIN      
        1 ctcctgccgg cgttccgccg tgcagaatgt gctaaagatg cagaaattcc aaggatgaga
       61 aaatgagttg tccgtggtca ggagaccacg aacgaacaat tgaagctata aactcaactg
      121 gcggtgcaaa aagtgcaggg cagggatgcc ctgcaccagt agcaatgttc gctgagacgt
      181 ctcgccgaag ctaaaaaaca ccctacgcaa acgcggattt gcacgtaact ccatctcgtc
      241 ccggcatccc gccgtggacc ttattttgat aaacagatct acttagttta agaaacagac
      301 aaaaaataag caaaacaaaa agagccccag aaatgagact ctttttatgc cgagtcacag
      361 acttgaactg tggaccctgc gattttcagt cgcatgctct accaactgag ctaactcggc
      421 gtgaacaagg attctaaccg gggatggcaa gactgtcaag caaaaaagaa tttttggcat
      481 ggctaattgt gtaggatcac actccatatt agccaaggcc taaaaataca gcgaaaaatt
      541 ccggtatccg ttattgttat ttcgttttaa aaaatcaatg gcgagaaacc ataagctgct
      601 gtttttttca tttcaaactg tccagtaaga ttgaaaatcc aacgaaaaaa cagtctcatt
      661 tgtcagattc ccttgacact gtgtaaatta aaagttaaca tcaagcaaac gtttgcgcaa
      721 tcgttcagat aggatgtcac catggcaaga gttactatca gagacgtcgc aaaagaagcc
      781 ggcgtttcta ccacaactgt ttcacacgtc atcaacgaaa cccgatttgt tgaggaaaaa
      841 acacgcgcca aggtcaatga agcgattgct acactagggt accgtgtcaa tatcgctgcc
      901 cgaagcctga gagctggcag atcgcaaaca atcggtctca ttattccaga cgcagccaac
      961 ctcttttttg ccgaatattt acgtaaaatt gaagattttg gctttaaagc cgggtacaac
     1021 gtgattgttt gcaacactga caacgaaacc gataaagaag ctgcctatat tgatgcgctg
     1081 gtatccaaac aggtcgacgg cgttattttg atttcctcag gcagcgatcc ggaaacatta
     1141 aatccgttaa tcagccatga tgttcctttt gtaatcgctg accgcgaatt ttccgcagat
     1201 gtcgccgatg tagtttcgct ggataacgaa caggctggtt ttgatgtaac taattattta
     1261 cttaaccttg gccatcgcaa gattggctgc atcaccggtc cgaacaattt ttcgcccagc
     1321 atccaacgcc ttaacgggtt tatccgtgct cacaaagaat gggggatccc ttacgatccc
     1381 gaactcatca ccagcgggaa gtttaccctt tcgagcggtt tctctgcctt cgactggatc
     1441 tggcagaata atagcaaacc aagcgcgctc ttcgtcttct ccgatatgat ggcgattggc
     1501 gcgatcaaca acgcgattac caaggggctg aatgttccca aggatatttc tattgtcggt
     1561 ttcgataata ttgaactggc agaagctttt gctccatcaa tcaccacctt caatcaacct
     1621 attgaaaaga tggctgagac catcgttgaa cttttaactg atcgaattaa acacaaatca
     1681 gcaaatagac gaagaatcgt tttccgcggt tctttgatta ttcgcgaatc tacaacaaaa
     1741 tattaggaga gatatgaatc gaattagtgt cattggcagt ttgaacatgg acttggtcat
     1801 ccagacagag cgagtgcctt tggcgggtga aacgatttcc ggtcgtgatt tcaacctgat
     1861 tcccggtggc aaaggcgcca atcaggcgat tgcggcagcc aaagccggaa gcccaacatc
     1921 tatgtttggc tgtgttggcg atgacgaatt tggtgaagtg ctgctaaact cactgaaaga
     1981 tgccggagtt aacaccgtta atgtgagcaa aacttcaaaa gttcgcaccg gtgttgcttc
     2041 aattattctt gaaagctctg gcgaaaaccg cattatcatc atcagcggtg ccaacaatct
     2101 ggtagacgcg gcttatgtcg acagccactg ggacgaaatt aagctatcct ctttgatctt
     2161 attgcagcat gagattcctt tggaaacctg tcattacatt attgaaaaag cgcatgcttc
     2221 ttcgattcct gtcatgctta accccgcgcc attccatgag attccaactg atgtcctgac
     2281 aaaagtcgat tatctggttg taaatgaagt cgaactacaa ggcattgtgc cggcccaggt
     2341 cagttcaatc gaagatgtca tccgcgcagg taaaaagatt aaccagatgg gcgtaaaaca
     2401 agtacttatc acactgggcg gggatggcgc cgtgctggtt agcaatgaag aagtcatcca
     2461 ccaaccagct tttaccgtca atgcggtaga taccaccgca gccggagata cttttgtggg
     2521 gtatttcgct tcggcaatta ttaatggctt tggtttgaaa caagctttgg tcaactcctg
     2581 cggcgcagcc gccttggcag ttaccaaagt tggcg
//
LOCUS       DAOSFC010000206         2614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_38038_length_2614_cov_6.969129, whole genome shotgun sequence.
ACCESSION   DAOSFC010000206 DAOSFC010000000
VERSION     DAOSFC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38038_length_2614_cov_6.969129"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PK791_05535"
     CDS             <1..1347
                     /locus_tag="PK791_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92808.1"
                     /translation="SGGSLMASFLERVRDRLAWFNGNASETTTDSKADTPDTEPERTQ
                     LALVENENTLMLGTRRFGDTDYDRLPYDRQELMENCLEAWRFNPLARRIIELTTQYVI
                     GAGMTLSADDTKAEAFLHSFWCHPLNKMDLKLSELSDELARTGNLFLMLSSDPSGMSF
                     ARVVPSTDVLSIHSRANDVEQELTYTLRGAHGEEDYLVPAWRADRPATQQNDVCILHY
                     AVNRPAGAQWGEPDLAPVLRWLARYSAWLEDRVRLNHFRNAFVYVVKARFANENARRM
                     RQMQLASQPPNAGSILVCDESEEWSVLSPKLEALDASTDGLAIKKMIATGVGIPLHFL
                     AEPESSTKTTAESAGHPTYRRFEERQRVLCWILEDLLRVVLKRKAVFDTSINPNAKII
                     VRGGDIHSRDNLELARAGHQMAVIALRMVEKGMIDAPEALRLIYRFIAEKAPETNP"
     gene            1422..>2614
                     /locus_tag="PK791_05540"
     CDS             1422..>2614
                     /locus_tag="PK791_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92809.1"
                     /translation="MTQHRHRLALEVNPISPDSFEILAITAGTANGWEFSAEVLRESL
                     ALWDGVNCFVDHDLVARSVRDIAGVLRKPSWDENAQGVRAELQAFGPSAHVLSDIGRQ
                     ILEISDEPAVKVGFSADVLFKGTNGKVEKILRIFSVDLVYNPARGGIFLRALNQLGIK
                     PIINGEKKMNQIQEQDTLELQSETGSAEDLSQDLQSQLSEIRNMRREMSAFLLESSLA
                     NTNLPPSMQSHIRERFQGRTFAPAELQSAIKEHQKLLSELDGGRSVKGHARVEAMLEP
                     AERLQAATDDLFGAPRDKALENARVARLSGIRELYLTLTGDHDLHGGYYPGRVQLATT
                     ADFSGLVKNSLNKLVANTWEELGRAGYDWWKQVTVQEHFNSLHDITGTLVGTVGDLPL
                     IAEGG"
BASE COUNT          619 a          665 c          771 g          559 t
ORIGIN      
        1 agcggaggca gtctaatggc atccttcctg gaacgcgtcc gcgaccgttt ggcatggttt
       61 aacggaaacg caagcgaaac cacgacagac agcaaagcag atacgccaga tacagaaccc
      121 gagagaacgc aactggcact tgtggagaat gaaaacacgc tgatgctagg cacgcgccgt
      181 tttggtgaca cggactatga ccgtctgcct tatgaccggc aggagctgat ggaaaactgt
      241 ctggaagcct ggcgtttcaa ccccctggca aggcgcatta ttgagctgac cacgcaatat
      301 gtcatcggtg ctggcatgac tttgagcgct gacgacacaa aagctgaagc ttttttgcac
      361 agtttttggt gccatccgct gaataaaatg gacttgaaac tgtccgaact ctccgatgaa
      421 ctggcgcgca cgggcaattt gttcctgatg ctgtccagtg accccagcgg gatgagtttc
      481 gcgcgtgtcg tgcccagcac cgacgtgctg agcattcact cgcgcgcgaa tgacgtggag
      541 caagagctga cttacacctt gcgtggcgcg catggcgaag aggactatct ggtgccagcc
      601 tggcgcgcgg accgtcctgc cacgcaacaa aacgacgtgt gcattttgca ttacgcggtc
      661 aaccgacccg ccggcgcgca gtggggcgag ccggatttgg cgccggtatt acgctggctg
      721 gcacgctatt cggcttggct tgaagaccgc gtgcgcctca accactttcg caacgctttt
      781 gtctatgtcg tcaaggcgcg ttttgccaac gaaaacgccc ggcgcatgcg ccaaatgcaa
      841 ctggcaagcc aaccgcctaa tgcagggtcg attttggttt gcgacgagag cgaggagtgg
      901 tcggtgcttt cgcccaaact tgaagcgctg gacgcgtcca cggacggtct ggcgattaag
      961 aagatgattg cgactggtgt gggtatcccc ttacactttt tggcggagcc ggagtcatcg
     1021 accaaaacga ccgctgaatc cgccgggcat ccaacctatc gccgatttga agagcgtcaa
     1081 cgcgtcctgt gctggatttt ggaagatctt ttgcgcgtgg tgctgaagag aaaagcggtc
     1141 tttgacacca gcatcaatcc caacgcgaaa atcatcgtgc gtggtggcga tatccattcc
     1201 cgtgacaatc tggaactggc gcgcgcgggg caccaaatgg cggttatagc actgcgcatg
     1261 gttgaaaaag gcatgattga cgcgcccgaa gccttgcgcc ttatttatcg tttcatcgct
     1321 gaaaaagcgc cggaaacgaa tccctgattg cgaaaaacaa caccaggact tgacgtgtgg
     1381 ggcgcttgtc ccggttttga aatggttttg aaaggagtct tatgacacaa cacaggcatc
     1441 gcctggcatt ggaagtcaac ccgattagcc ccgacagctt tgaaattttg gcaattacag
     1501 cgggaacggc taacggatgg gaattttccg cggaggtttt gcgcgagtcc ctggcattgt
     1561 gggacggtgt gaattgcttc gtggatcacg atttggtggc acggtcagtg cgcgatatcg
     1621 ctggcgtttt gcgtaaaccc agctgggatg aaaacgcgca aggcgttcgc gcggaattgc
     1681 aggcttttgg gccttccgcg catgtgctga gcgatatcgg acgtcagata ctggaaatca
     1741 gcgatgaacc cgcggtaaag gtgggtttct ccgcggatgt gctttttaag ggcacgaacg
     1801 ggaaggtcga gaaaattttg cgtattttct cggtcgattt ggtctacaac ccagcgcggg
     1861 gcggaatctt tttgcgcgcg ctgaaccaac tgggcataaa acccatcatc aatggagaaa
     1921 aaaagatgaa tcaaattcaa gaacaagata cgcttgaact gcaaagcgag acgggttccg
     1981 cggaagacct aagccaggat ttgcaaagcc agctttcgga aattcgcaat atgcgccgtg
     2041 agatgagcgc tttcctgctg gaaagttcgc tggcgaacac aaatttgcca ccgagtatgc
     2101 aaagtcatat tcgcgaacgc tttcaggggc gaactttcgc gccggcggag ttgcagagcg
     2161 cgattaaaga gcatcaaaaa cttttgagcg aactggatgg cgggcgttcg gtaaaaggac
     2221 acgcccgtgt ggaagctatg ctggaacccg ccgaacgttt acaggctgct acggatgacc
     2281 tttttggcgc gccacgcgat aaagcgctgg aaaacgcgcg ggtcgcgcgt ctgagtggca
     2341 ttcgcgagct ttatctcacc ctaaccggcg accacgatct gcacggtggt tattatcctg
     2401 gccgtgtgca actggccacg accgcggact tcagcggact ggtaaaaaac agtctcaaca
     2461 aactggtggc aaacacctgg gaagaactcg ggcgcgctgg atacgactgg tggaagcaag
     2521 ttaccgtgca ggagcatttt aacagcttgc acgacatcac cggcacgctg gtgggcacgg
     2581 taggcgattt gcccttgatt gctgaaggcg ggaa
//
LOCUS       DAOSFC010000207         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_38228_length_2606_cov_6.405331, whole genome shotgun sequence.
ACCESSION   DAOSFC010000207 DAOSFC010000000
VERSION     DAOSFC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38228_length_2606_cov_6.405331"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PK791_05545"
     CDS             complement(<1..714)
                     /locus_tag="PK791_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92810.1"
                     /translation="MKKFLKTFLTLIILGGLMALPGLIPSQMQSVQAENLQQEETPVV
                     PTDPPGTATRPLLYIQSYQTRDGGYKNVSPWGEFQLTFTLNNNGKAHARNIVMTFSSQ
                     VFDPLDGSVITFWEVDADGAGPETRTHRFKVNDMQAWMYSGTIVATTTYTDEAGNSYS
                     DTFTFTITIDQQPSTGSATATPTSKPINRPQMIINSYETDIVPLQPGTTFKLKMSVTN
                     AGNADARAVSVVFGGGANAT"
     gene            complement(726..1442)
                     /locus_tag="PK791_05550"
     CDS             complement(726..1442)
                     /locus_tag="PK791_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92811.1"
                     /translation="MMSENEFIKVSDLYKIYKMGNQEVRALDGLSLELKKGSFNVLMG
                     PSGSGKSTLLYLVGGLDWPTSGHIVVDGEQIDQMDENQLAQYRREKLGFIFQSFNLIP
                     SMTAVANVAFPLRFSGVKKAERTKRALDLLQRMGLGERSDHKPTELSGGQQQRVAVSR
                     ALVNDPDVILADEPTGNLDTTSGTIIMDLLKELREEGKTILVATHDPRMLQYASNAIF
                     LIDGKEVSEENYRKIIQQQS"
     gene            complement(1447..2241)
                     /locus_tag="PK791_05555"
     CDS             complement(1447..2241)
                     /locus_tag="PK791_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOH92812.1"
                     /translation="MSETIMASEVNIKEKEQGSQWAWVPGILFKPRSTCKKIVSKDAA
                     VWHIPLLILSVLAILVIIASAPIKRYNAQMGASMPEDSRWWSEEQIAKYLEAQQNMTS
                     GTFMYLFPTISKLIGIWFNWLIFSSILYLALTLAGSRAPRLKSGNLVAWAMLPLAFRY
                     IVELILIFSRNQLPSGTALDSLIGETKSIGLSLVKGMLGQIDAYWLWFVIILLIGGVS
                     LAGIKRSKAFWVITLVILIMLVIQGVPTMISQYLGQLTGSGFNMYF"
BASE COUNT          682 a          600 c          609 g          715 t
ORIGIN      
        1 tgtggcgttg gcaccgccac caaaaaccac acttaccgcg cgcgcgtccg cattaccggc
       61 gttggtaacg ctcatcttga gtttaaaggt cgtaccgggt tgcaggggta cgatgtcggt
      121 ctcgtaagaa ttgataatca tttgcggccg attaatgggt ttgctggtgg gtgtcgcggt
      181 ggcagaacct gtacttggct gttggtcaat tgtgatggtg aaagtgaaag tgtccgaata
      241 actgtttcct gcttcgtcgg tgtaggttgt ggtagccacg atagttccgg aatacatcca
      301 ggcttgcata tcgttgacct taaaacggtg cgtgcgcgtt tctggcccag caccgtcggc
      361 atccacttcc caaaacgtaa tcacgcttcc atccagtggg tcaaagacct gactgctgaa
      421 agtcatcaca atattgcgcg cgtgcgcttt gccgttgttg ttaagtgtga atgttagttg
      481 aaattcaccc catggcgaaa catttttgta accaccatca cgggtttgat agctttgaat
      541 ataaagcaaa ggtcgtgtcg cggttccggg tgggtctgtg ggcacgacag gtgtctcttc
      601 ttgttggagg ttttctgcct gcacgctttg catttggctg ggaatcaagc ccggcaaggc
      661 cattaaaccg ccgagtatta taagggtcag gaatgttttt aggaattttt tcatacaatc
      721 accttctaac tttgttgctg aattattttt cgatagtttt cttcgctaac ttctttgcca
      781 tcaatcaaaa agatggcatt gctggcgtat tgaagcatgc gcgggtcgtg tgtggcgact
      841 aaaatcgtct tgccttcttc gcgcagttct ttgagtaagt ccataatgat ggtgccgctg
      901 gtggtatcca aattgccggt tggctcatca gcgagaatca cgtcagggtc gttgaccaga
      961 gcgcgcgaaa ccgccacacg ttgttgctgc ccaccggaga gttcggttgg tttgtggtcg
     1021 ctacgctcgc caaggcccat tctttgcagt aaatccagcg cgcgttttgt acgctcagct
     1081 tttttgacgc cactaaaacg cagtggaaaa gctacattcg ccacggcggt catactgggt
     1141 atcagattaa aactttgaaa gatgaaacca agcttttcgc gacggtactg ggcgagttga
     1201 ttctcgtcca tttggtcaat ctgttctcca tcaaccacaa tatgccccga tgttggccag
     1261 tcaagcccgc cgaccagata aagcagggtg ctttttcccg aacccgaagg gcccatcaag
     1321 acgttaaatg aacctttttt gagttccagg ctaaggccat cgagcgcgcg gacttcctga
     1381 ttgcccatct tgtagatttt gtataagtcg cttactttaa tgaattcgtt ttcgctcatc
     1441 atgtccctaa aaatacatat tgaaaccact gccagtaagc tgaccaaggt actgcgatat
     1501 catcgtcggc acgccttgga ttacaagcat aatcaggata accagagtga ttacccaaaa
     1561 ggctttcgag cgtttgatgc ctgccaaaga cacaccaccg attaacaaaa taatcacaaa
     1621 ccacagccag taagcgtcaa tttgtcccaa catgcctttg accaagctca gtccgattga
     1681 ttttgtctcg ccaatcagag aatccaaagc cgtgccactg ggaagttgat tgcgactaaa
     1741 gatgagaatg agttcaacga tatagcgaaa agccaggggc agcattgccc aggctaccag
     1801 gttccccgat ttgagtcttg gcgcgcgact acccgccagg gtgagagcca ggtacaaaat
     1861 actgctaaaa ataagccagt tgaaccagat gccaatcagt ttgctaatag tggggaagag
     1921 atacatgaat gttccggaag tcatgttttg ttgcgcttcc agatatttgg caatttgctc
     1981 ttcggaccac cagcgtgagt cttcgggcat actggcaccc atttgcgcgt tataacgttt
     2041 gataggagca gatgcgatga taaccaggat ggccagcaca gacagaatta gcaaagggat
     2101 atgccaaacg gcagcatctt tgctgacgat ttttttacac gtgctacgag gtttaaacag
     2161 aatgccaggc acccaagccc attgactgcc ttgctctttt tccttaatat ttacttcgct
     2221 tgccattatc gtttcactca tcggcatgct ccaaatattc gtcacgcata tttaataaga
     2281 cgctgcgcga agcgtttttg ttccgcattg attatagtcc tttttaaaaa atgcaaaatc
     2341 agactttagt tgtatttatc tttgaagcaa aaaaactcaa acagtaagat attggtctaa
     2401 aatatgcaaa aataaccgtg taagcagtaa atccatttta taatcataaa agcttattca
     2461 aattaattaa aggagtatgc ttatgagaca aaaacttaaa caacaaattg accagctgaa
     2521 agccaaaggt gctacatacg ccgatgtgcg ctggtatcct tatgaggacc aaaacgttct
     2581 cgttatgcat aatggcaact taaaag
//
LOCUS       DAOSFC010000208         2605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_38248_length_2605_cov_4.794118, whole genome shotgun sequence.
ACCESSION   DAOSFC010000208 DAOSFC010000000
VERSION     DAOSFC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38248_length_2605_cov_4.794118"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PK791_05560"
     CDS             <1..1058
                     /locus_tag="PK791_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOH92813.1"
                     /translation="KGRRNVGVTGLTTVFDSGIPGNNILLRFDMDGLPIPEETGLSFE
                     SQNKGAMHACGHDGHMAIGLSIARYLAQNPDEITGKVYFVFQPAEEIGQGAKAIIADG
                     LFDAISVDYALAVHLWAEQAYGWLGIPDGPLMAGSSDIRIEIFGKGGHAAKPDLTIDP
                     LYIAAKVINTLKDVYPGDDTAYQTGLVTLTQLEASDRRNIIADKVTMAGTARWFDDDM
                     RKALHKGITKIVNGISRQFGAHANITFKESTVPVVNDKKVTMVCRQIAKELRVDKVNV
                     IVDDKYRTNLSEDFAYILNQVPGAMLLVGARKELDGNAYPHHHPKFNIDERAMTLAVA
                     ILLETVKNLTAHENEWL"
     gene            1055..1792
                     /gene="tatC"
                     /locus_tag="PK791_05565"
     CDS             1055..1792
                     /gene="tatC"
                     /locus_tag="PK791_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOH92814.1"
                     /translation="MTDEKQSLLDHIEELRKTLLKILIGLVVGVVVAAFFVKPILKFL
                     ATPIGGLEKLHAIEVTESVGVYMRIALLAGLLLALPWIFYQLFRFVGKGLTEKERKTI
                     LIAVPFATLMFLGGVTFAYFIMLPASLDFLIGILGINTMIRVKSYYSFLTNLLFWIGI
                     SFEMPLIGYVLARFGLLKAKVMLKGWRVAIVVIAILAAVITPTSDPVNMAIFMIPLFV
                     LYLLSIGLAAIAEKQRVKTKAATTTEV"
     gene            1793..1990
                     /locus_tag="PK791_05570"
     CDS             1793..1990
                     /locus_tag="PK791_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOH92815.1"
                     /translation="MPQLGIWEVLIIILIIVILFGAGRIAKAGGELGKGIRAFKEGLQ
                     GKDSETTDQDDAEIDSAKEDK"
     gene            2008..2370
                     /locus_tag="PK791_05575"
     CDS             2008..2370
                     /locus_tag="PK791_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOH92816.1"
                     /translation="MEFFNIGGLELLLILLIALLALGPKRIVEVGHKLGNAMRKLSKN
                     LLFQEIVQTTDEIRNYPRKIMDEAKLDMSDLDMTLKIRDENDVLDKSLNGKSTFSQED
                     NISNTKQNLVSEQQAKTE"
     gene            complement(2471..2547)
                     /locus_tag="PK791_05580"
     tRNA            complement(2471..2547)
                     /locus_tag="PK791_05580"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2511..2513),aa:Asp,seq:gtc)
BASE COUNT          781 a          465 c          616 g          743 t
ORIGIN      
        1 ataaaggacg caggaacgta ggtgtcacgg gcctgacaac tgtgtttgat agcgggatac
       61 ctggcaataa tatattgttg cgttttgata tggacggatt gcctattccg gaagaaaccg
      121 ggctttcttt tgaatcgcaa aacaaaggtg caatgcatgc ctgtggacat gacgggcata
      181 tggcaattgg attaagtatt gcccgatatc tggcgcaaaa tcctgatgaa attacaggta
      241 aagtttattt tgttttccaa ccagcagaag aaattggcca aggcgcgaaa gcgataattg
      301 cggatggttt gtttgatgct atctcggttg attatgcatt ggcagtacat ctttgggctg
      361 aacaagctta tggctggttg ggcatacccg atgggccttt gatggctggt tccagcgata
      421 ttcgaattga aatatttggt aagggcggcc acgctgcgaa acctgatttg acaatagacc
      481 cgctttatat cgctgccaag gtaataaata cactaaaaga tgtttatcca ggtgacgata
      541 cagcttacca aacaggtttg gtgactctga cacaactcga agctagtgac agacgaaaca
      601 tcattgctga caaggtcaca atggctggta ctgcccgttg gtttgatgat gatatgcgaa
      661 aagcgcttca taaagggata actaaaattg taaatggaat ttcccggcag tttggagcgc
      721 atgctaatat tacgtttaaa gaaagcactg ttcctgttgt aaacgataag aaagtcacca
      781 tggtttgccg tcaaatagcg aaagagctgc gcgtagacaa agtcaatgtg attgttgatg
      841 ataaatatag aacaaatttg tctgaagatt ttgcatacat attgaatcaa gttcccgggg
      901 cgatgttgct ggtaggtgcc aggaaagagc tcgatggcaa tgcttatccg catcatcatc
      961 caaaatttaa tatcgatgaa cgtgcgatga ccttggcagt agctatttta ctggaaactg
     1021 tcaaaaatct aacagctcat gagaatgaat ggctatgact gacgaaaaac aaagtttact
     1081 agaccatatt gaagaactac gaaaaacgct tttaaaaata ttgattggtc ttgtagtggg
     1141 tgtggttgtg gctgcattct ttgttaaacc gattcttaaa tttttggcaa cgccgattgg
     1201 tggccttgaa aagcttcacg ctattgaagt tactgagagt gttggcgtat acatgcgcat
     1261 cgcgcttttg gcaggtttat tgttggcttt gccatggatt ttctatcaat tatttcgatt
     1321 tgttgggaaa ggactgacgg aaaaagaacg taagacgata ttaatagctg tgccctttgc
     1381 cacgctgatg tttcttggag gcgtcacatt cgcatatttt attatgctcc ctgcttcact
     1441 tgatttttta ataggcattt tgggaataaa tactatgatc agggtcaaat cctattacag
     1501 tttcttaact aatctgcttt tttggattgg gataagtttt gaaatgccgc ttattggtta
     1561 tgttttggca aggtttggct tgcttaaagc caaagtaatg ctaaaaggct ggcgggtggc
     1621 gattgtcgtt atagccattt tagcagcggt tatcacacca accagtgacc ctgtaaacat
     1681 ggcaattttc atgataccgc tttttgtatt atatttattg agtattggat tagccgctat
     1741 cgcagaaaaa caacgcgtga aaaccaaagc ggcaactaca acggaggttt aaatgccaca
     1801 attaggaatt tgggaagtat taatcattat cttgattatc gttattcttt ttggtgcggg
     1861 acgtattgcc aaagcaggcg gtgaactggg taagggtatt cgcgctttta aagaaggttt
     1921 gcaaggtaaa gacagtgaaa caaccgatca ggatgacgca gaaatagatt ctgccaaaga
     1981 agataaataa gctgatatta gggagaaatg gaatttttta atatcggtgg cctggagctg
     2041 ctgcttattt tacttatcgc cttactggca cttggaccaa agcggattgt tgaggtgggg
     2101 cataagcttg ggaacgccat gcgcaaattg agcaaaaacc ttcttttcca ggaaatagtg
     2161 cagacgaccg acgaaatccg aaattatccg cgaaaaatca tggatgaggc gaaactggat
     2221 atgtcagatt tggatatgac acttaagata cgggatgaaa atgacgtctt agacaaatct
     2281 ttgaatggaa agtcgacttt ttctcaagaa gataacattt cgaatacaaa acaaaatctg
     2341 gtgagtgaac agcaagccaa aactgaataa tttacaaata ataatgataa aggctctgtg
     2401 agcccatagc ctgaaaagaa tcgcataatt ccctgtaaaa taaaaagctg tcccaaagga
     2461 cagctttttt tggcgggagc gacgggattt gaacccgcga tctcggcctt gacagggccg
     2521 catgttaaac cgctacacca cgcccccgag tagcaaagag tataacacag caattttata
     2581 agtcaagctt tttttcactg tccaa
//
LOCUS       DAOSFC010000209         2589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_38583_length_2589_cov_3.986188, whole genome shotgun sequence.
ACCESSION   DAOSFC010000209 DAOSFC010000000
VERSION     DAOSFC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38583_length_2589_cov_3.986188"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PK791_05585"
     CDS             <1..547
                     /locus_tag="PK791_05585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH92817.1"
                     /translation="RLADGRELPADLVVISAGVRPNLSLALSAGLKTNRGIVVDSAMR
                     TSVENILAVGDVCECNGKVYGQWIPANTQGKIAGLTATGKDSTFEDWSPAALLKVMGF
                     NLFSIGKIAPTEPDEFVIAGEKEGNYYSILFGQGKVLGASLIGDTAPSRIIKRALENG
                     DDFSDKVQAETSLQEMLLLFS"
     gene            complement(578..1693)
                     /locus_tag="PK791_05590"
     CDS             complement(578..1693)
                     /locus_tag="PK791_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH92818.1"
                     /translation="MIICPNCKSQCTPDHNFCPACGAKMPQAASQTQQQGTYYTGAQQ
                     AGYSYPQATYQPPAPKKKSNVWLIVVIAVVAVICIVSTILLISYIRKIKGKSTDLLQT
                     ATAIHSALETDIHGQENIWQTEEALLTTINDPARTATAFAQEIESAQTQAVRTQEALE
                     AQTQATQTQEAYYIEQTASAQAAQEQSVLALLPEDVRPLVENATKVDAPTYLEEVYIH
                     DKWLETVWVAGDIKNFVLTVTFDNPIDVRVNDYWEMGILFRSKAANDQLRLFVEVPKY
                     WELSYVGGSGGYWSTVRSWTATINALDLSEGGSNTITLISKGHKGSFYINGVFISDLD
                     LSSRANYGGISLWIEYNTMARNNEKTIFRDIHLWDLD"
     gene            1844..>2589
                     /locus_tag="PK791_05595"
     CDS             1844..>2589
                     /locus_tag="PK791_05595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH92819.1"
                     /translation="MSKTALITGATGGLGEVFAELFAADGIDLVLTARNLPKMEEIAE
                     RIRTKYDVKVELIPQDLSVETSAEILYEDLSSRGFEINFLVNNAGFGDFGRLIDIDWR
                     RERDLIGVNLIAPVRLTQLFAKDMVARGCGRVLNVASIAAWTPGPYMNGYFASKAYVY
                     SFSQSVHQELKGTGITVTALCPGPTQTGFEKNAEMKNSNMFKALKVMDARFVAKVGYR
                     DMMKGKPISVPDWYYKVYKFILRFAPECLAR"
BASE COUNT          676 a          575 c          635 g          703 t
ORIGIN      
        1 gcgcctggcg gatggacggg aactgccggc agatttggtg gtcatcagcg cgggcgtcag
       61 gcctaatctc agtttggcgc ttagcgcggg cctaaaaacc aaccgtggca ttgtggtgga
      121 tagcgccatg cgcacgtccg ttgaaaacat tttggcagtg ggcgatgtgt gtgaatgtaa
      181 cggcaaggtt tacggacagt ggattcctgc caacacgcaa ggcaaaatcg ccggcctgac
      241 cgcaacaggc aaagacagca catttgaaga ctggagcccc gcggcactct tgaaagtaat
      301 gggctttaat ctctttagca tcggaaaaat cgctccgacc gagccagatg aatttgtgat
      361 tgccggcgaa aaagagggta attattacag tattttgttt ggccagggaa aagtacttgg
      421 cgcaagcttg attggcgata cggcaccatc cagaataatc aaacgcgccc ttgaaaacgg
      481 ggatgatttt tcagataaag ttcaagctga aaccagctta caagagatgt tgctcttgtt
      541 tagctagttc cagcttgatg aattcaagtc ttagggatta gtccaaatcc caaaggtgaa
      601 tatcgcggaa gattgttttc tcgttattgc gcgccattgt gttgtattct atccacagac
      661 taatgccacc gtaatttgcc cggctgctaa ggtctaagtc gctaataaaa acaccgttga
      721 tatagaaaga acctttgtgc ccttttgaaa tgagtgtaat cgtgtttgag ccaccttccg
      781 agagatcaag tgcgttaatt gtagcagtcc aactcctgac cgtactccaa tacccccctg
      841 aaccaccaac ataagagagt tcccagtatt tgggtacttc cacaaaaaga cgcaactggt
      901 cattagcagc ttttgagcgg aacagaatgc ccatttccca ataatcattt acccttacat
      961 caatcgggtt gtcaaaggtt acggtaagca caaagttttt gatgtcacct gctacccaaa
     1021 cagtctccag ccatttgtcg tgaatataaa cctcttccag gtaagttgga gcatcgactt
     1081 ttgtggcatt ttcaacgagc ggacgaacat cttcaggaag aagcgctaaa accgattgct
     1141 cttgcgcggc ttgggcactt gcggtttgtt ctatataata cgcttcctgg gtttgggttg
     1201 cttgtgtttg cgcttccaga gcttcctggg tacggaccgc ttgcgtttga gcgctttcta
     1261 tttcttgagc aaaagctgtg gcagtgcgcg caggatcgtt aatcgtcgta agtaaagctt
     1321 cttctgtttg ccatatattc tcctgaccat gtatgtcagt ttctaacgca gagtgaatag
     1381 ctgtggcggt ttggagcaaa tcagtcgatt tgcccttaat ttttcgaata tatgagatga
     1441 gaagaatggt gcttacaata caaattacag ctactaccgc aatgacgaca atcaaccaaa
     1501 cattgctttt ctttttgggc gcaggcggtt ggtaagtagc ttgtggataa ctataacctg
     1561 cttgttgcgc tccggtgtaa tatgtgcctt gttgctgagt ttgagatgca gcctggggca
     1621 tcttggcacc acatgccgga caaaaattat ggtctggggt acattgactt ttacagttag
     1681 gacaaattat catttttgcc tccttaatac aagatgataa ttagctaaat tatacatcta
     1741 aagtacagaa aatttttgtt tttataccca caaatcgcgt ttaaaagaaa aaataaaaag
     1801 aatgcattac acttaagcaa aatgaccttt cctggaggaa aagatgagta aaacagcttt
     1861 aattaccggc gcgacgggtg gacttggcga ggtttttgcc gaactttttg ctgccgatgg
     1921 cattgatttg gttttgaccg cacgcaatct gcccaagatg gaggaaatag ctgagcgaat
     1981 tcgcacaaag tatgatgtaa aggtggagct tatcccccag gatttaagcg ttgaaaccag
     2041 cgcggagatt ctgtatgaag atttaagttc gcgtgggttt gagattaatt ttctcgtcaa
     2101 caacgcgggt ttcggtgatt ttgggcgctt aatcgatatc gactggcgac gcgagcgcga
     2161 cttaatcggc gtcaatctta tcgcgcctgt ccgcctgacc cagctttttg caaaggacat
     2221 ggtcgcgcgt ggctgtggac gggttttgaa cgtcgcctcg atcgccgcct ggacgcccgg
     2281 tccctatatg aacggttatt tcgcgtcaaa agcatatgtg tactcgtttt cgcaatccgt
     2341 gcatcaggaa ctaaagggaa ccggcatcac tgtaacagct ttgtgtcccg gaccaaccca
     2401 aaccggtttt gagaaaaatg ccgaaatgaa gaactccaac atgttcaaag ctctaaaagt
     2461 catggacgcg cgctttgtgg ccaaggtggg ctatcgcgac atgatgaaag gcaagcccat
     2521 ctccgtgccg gactggtatt acaaggtcta taaattcatc ttgcgtttcg cgccggaatg
     2581 cctcgcccg
//
LOCUS       DAOSFC010000210         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_38852_length_2576_cov_5.437921, whole genome shotgun sequence.
ACCESSION   DAOSFC010000210 DAOSFC010000000
VERSION     DAOSFC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38852_length_2576_cov_5.437921"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PK791_05600"
     CDS             complement(<1..997)
                     /locus_tag="PK791_05600"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH92820.1"
                     /translation="MTRDIIKTMDSEGISHIDSKDLTENREQLTRENWFNKTWKRLVR
                     FGLHDTARRIVVMVSTMLLMVLVLAFMNRFFVSLSGQKPSEIGSSSALAEEIEGLKLL
                     PDFEINQNAHSRKAIPRDTEMTTILPERSRSGIIEHIIQPGESLFSISAKYNIRPETL
                     LWSNRYTIGDDPHAMIPGQKLLILPIDGILHRWSAGEGLNGVADFYNVSPDVIVNYPP
                     NGLDMASIGDFAEPNIEPGKLIVIPGGTAAFSDWRTPRITRKEPATAKNVGPGACEGE
                     FDGVIGAEKFTFPVASDPVLSGFDFQPAANHFGIDLGGAIGDPISAVDNGVVVYAG"
     gene            complement(1063..1755)
                     /locus_tag="PK791_05605"
     CDS             complement(1063..1755)
                     /locus_tag="PK791_05605"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HOH92821.1"
                     /translation="MRDTIYHYYAFDTVKLLVAGGDGATWVRNSFEYVGLPKVDLLDR
                     FHVIRAIIQTWGKDMDIGLLLSDLFTKGWYMVRGQLLANIAKSKGENKKKKLKTYKYL
                     LNNRDGLLDLDKRGLPVKDLCTLGAAEGNVDKLVRQRMRGRGCCWSWSGARAMLAVLP
                     HVQVLSQHVFEYRPVTVGQCKKQRIKKTEGNGYQPVSGSLSYLFSHTSQEKWVKCIKA
                     YLNSNLSLIEFF"
     gene            complement(1758..2474)
                     /locus_tag="PK791_05610"
     CDS             complement(1758..2474)
                     /locus_tag="PK791_05610"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HOH92822.1"
                     /translation="MTEQTVQIIITLKVKENDLSFERPIDITNLEKDIGSFSQEIGSQ
                     LLSMMLKMLDDMLRDIVPDDWKNVGTERRSVQFECGYVHFKRRIYQDTEGARHKPLDE
                     LLELEPWQRSSRGLEEMSSALAATNTYRQASNLISWLVKAAISPSTIGRYLRKTGQRI
                     QEQERMATEGEEQGQIETEVLYAEADGVYISLQREKKKRAEVKVSIIYTGKEAIGKGR
                     NRCTTRYVSRSWVAATRNGR"
BASE COUNT          626 a          668 c          527 g          755 t
ORIGIN      
        1 agcctgcgta taccaccacg ccattatcca ccgcgctaat tgggtcacca atcgcgccac
       61 ctaagtcaat accaaaatgg ttggcggctg gctgaaagtc aaaaccgctc agaaccggat
      121 cactcgcgac cggaaaagtg aatttttcag caccaattac accatcaaat tcgccttcac
      181 aagcaccagg acccacgttt ttggctgtcg ctggttcctt gcgcgtgatg cgcggcgtgc
      241 gccaatcgct gaaagccgct gtcccgccgg ggatgacaat tagcttacca ggttctatat
      301 ttggttcggc aaaatcgcca atgcttgcca tatctaatcc attaggcgga taattcacaa
      361 tcacgtccgg gctaacgttg taaaaatccg ccacgccatt caagccctca ccagcactcc
      421 agcgatgtaa aatgccatca atgggcagaa tgagtagctt ttgaccggga atcatcgcgt
      481 ggggatcatc cccaatcgta taacggttgc tccaaagcag ggtttcaggg cgaatattgt
      541 acttggctga aatcgaaaag aggctttcgc cgggttgaat gatatgttca attatgcctg
      601 aacggctacg ttctggcaat atcgtggtca tttccgtgtc acgcggaatc gctttgcgag
      661 agtgtgcgtt ttgattgatt tcaaagtcag gtaagagctt taatccctca atttcttctg
      721 ccaaagctga gctgctgcca atctccgaag gtttctgtcc gctgagactg acaaaaaaac
      781 gattcataaa ggcaagcacc aagaccatta acagcatggt actcaccatc accacgatgc
      841 gtcgcgcggt atcatgcaga ccaaagcgta ccaagcgctt ccaggttttg ttaaaccagt
      901 tttctctggt gagctgttct ctgttttctg tcagatcttt agagtcaatg tgtgaaattc
      961 cttctgaatc catcgtcttg atgatatcac gagtcaaaca ttttgatgaa tccccctgcc
     1021 aaagctttag gtggtagtta ctgtttcaca tttccctata atctaaaaga actcaatcaa
     1081 agagaggttg ctgtttagat acgccttgat acattttacc catttttcct gacttgtgtg
     1141 cgaaaacaga tatgacaaac ttccagacac aggctggtag ccattacctt cggttttctt
     1201 tattctctgt tttttgcact gcccaacagt cactgggcga tactcaaaca catgttgact
     1261 cagtacctga acatggggca aaactgcaag catagccctg gcaccagacc atgaccaaca
     1321 acagcctcta cccctcatcc tctgacgcac cagcttgtct acatttccct cagcagcgcc
     1381 cagggtgcac aggtctttta ctggcaaacc acgtttgtcc agatcaagca atccatcacg
     1441 gttattgagc aaatacttat atgtttttag ttttttcttt ttgttttccc ctttactttt
     1501 ggcgatgttg gcgagaagtt gccctctgac catataccag cctttggtga aaaggtcaga
     1561 taacagcaac ccaatgtcca tatctttacc ccaggtctgg attatagctc tgatgacatg
     1621 aaagcgatcc agcagatcaa ctttcggcaa gccaacatat tcaaagctgt tgcgaaccca
     1681 agtggcgcca tcaccaccag ccaccagcaa ctttacagta tcaaaagcat agtaatgata
     1741 gatggtatcc cgcattttta tctgccattc ctcgttgctg ccacccaact gcgtgataca
     1801 taccttgttg tacagcgatt tcgacctttc ccaatagctt ctttgcccgt atagatgatg
     1861 ctcactttga cttctgccct tttctttttc tctcgctgca gagaaatata cacgccgtcc
     1921 gcctcagcat acagcacttc tgtttcgatt tggccctgct cttcaccttc ggttgccatg
     1981 cgttcctgtt cctgtattct ctgccctgtt tttctcagat atcttcctat ggtgctggga
     2041 ctgatagccg ctttgaccaa ccaggaaatc aagtttgaag cttgcctgta ggtgtttgtt
     2101 gctgccagtg cgctactcat ctcttcaagg ccccggctgc tacgttgcca gggttccagc
     2161 tccaacaatt catccagtgg cttgtgtctg gctccttccg tgtcctgata gatcctgcgt
     2221 ttgaagtgga cataaccgca ttcaaactga acacttcttc tctctgtgcc gacgtttttc
     2281 cagtcatcag gcacgatgtc gcggagcata tcatccagca ttttcagcat cattgataat
     2341 agctgagagc caatttcctg actgaagcta ccgatatctt tttccagatt ggtaatatcg
     2401 attgggcgtt cgaagcttag gtcgttttcc ttgactttca gcgttataat aatttggaca
     2461 gtttgttctg tcatttgaga gttcctcctt attgtttttt aggcaaatcc aattatgaca
     2521 ggaactctct ttttttgtat gtccttttca agatagggaa atttgaaact gaaacg
//
LOCUS       DAOSFC010000211         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_39611_length_2543_cov_7.760048, whole genome shotgun sequence.
ACCESSION   DAOSFC010000211 DAOSFC010000000
VERSION     DAOSFC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39611_length_2543_cov_7.760048"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="rplC"
                     /locus_tag="PK791_05615"
     CDS             <1..633
                     /gene="rplC"
                     /locus_tag="PK791_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOH92823.1"
                     /translation="MLKGLIGRKIGMTQIFDESGRAIPVTLIEAGPCFVSQIKTPETD
                     GYSAVQLAFGAVKPKRLSKAELGHLKVNNLPPVRILREIRTKKIGDVKPGDTITADVF
                     ETGDYVDVIGTSKGKGFAGVMKRHGFGGGLKTHGQSDRWRAPGASGQGSTPGRVFKGK
                     KFPGQMGNVRVTSSHIRVALVDAERNLIAVQGSVPGAKGDTVIIRAAAKQ"
     gene            658..1287
                     /gene="rplD"
                     /locus_tag="PK791_05620"
     CDS             658..1287
                     /gene="rplD"
                     /locus_tag="PK791_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOH92824.1"
                     /translation="MKLDVYNLKAEKVSDVELPEEIFAAKVNVDLMHQAYQRQMANAR
                     LGTHNTKRRAEVRGGAGKPFKQKGTGRARRGSMKSAQHVGGGRIFTPKPRDYSQEMPR
                     KMRRAALRSALTVVANDEKIIMLDNLNMPEAKTAELAKSIKALAGDNRVLVLIPGNSE
                     ENENFIKSGRNLANARLLQARYLNIRDLLISDKVIIPLEALDVIKSYLG"
     gene            1302..1607
                     /gene="rplW"
                     /locus_tag="PK791_05625"
     CDS             1302..1607
                     /gene="rplW"
                     /locus_tag="PK791_05625"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOH92825.1"
                     /translation="MTISAFDIIRQPIVTEKSNYLAGQLQQYVFEVASFATREQVKTA
                     IETAFDVKVERVNIINAPAKSTRNSRSRRLTIRKRGYKKAIVTLRAGDRIPFFEGVE"
     gene            1607..2446
                     /gene="rplB"
                     /locus_tag="PK791_05630"
     CDS             1607..2446
                     /gene="rplB"
                     /locus_tag="PK791_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOH92826.1"
                     /translation="MAVKVYKPTSPGQRHKTSYTFEEITKTTPERSLIYTKKNRAGRN
                     AQGRITVRHRGGGSKRQIRIVDFKRNKHDIPAKVAAIEYDPNRTARLALLHYADGEKR
                     YILAPIGVKVGDTLFSGTQVEIRPGNNLPLANIPVGTTIHAIEMQPGKGAQIARSAGV
                     SAQLLAKEGRYAHVRMPSGEVRLVLQDCSATIGQVGNVEHNNIKLGKAGRSRHLGRRP
                     AVRGSAMSPRDHPHGGGEGKTGIGMAGPKTPWGKPTLGYKTRRNKRTDDMIIRHRSKA
                     KRR"
BASE COUNT          699 a          646 c          643 g          555 t
ORIGIN      
        1 atgttaaaag ggcttattgg aagaaagatc ggcatgaccc agatcttcga cgaaagcgga
       61 cgtgcaattc cggtgacgct tatcgaagct gggccttgtt ttgtttctca gataaagact
      121 cctgaaactg acgggtattc cgcagttcag ttagcctttg gcgctgtgaa accaaaacga
      181 ttgtcaaaag cagagcttgg ccatcttaag gtcaacaatc tgccacctgt gcgcatcctg
      241 cgcgaaattc gcacgaagaa aattggcgac gtcaaacccg gtgacacaat cactgcggac
      301 gtcttcgaaa cgggcgatta tgttgatgtc attggcactt caaaaggcaa aggttttgcc
      361 ggtgtcatga aacgccacgg ttttggcggt ggtctaaaga cccacggtca atccgaccgc
      421 tggcgcgcgc caggtgcgtc tggacagggt tcaaccccgg gacgtgtctt taagggcaaa
      481 aagttccccg gacaaatggg aaatgtgcgc gtgacatctt cgcatattcg tgttgcattg
      541 gttgatgctg agcgcaacct tatcgctgtt caaggctccg ttcccggcgc caagggcgat
      601 acagtgatca ttcgcgcggc ggccaagcaa taggacgtct gattggagag ataaatcatg
      661 aaactcgatg tctataactt aaaagcagaa aaagtaagcg acgttgaact gcccgaagaa
      721 atcttcgcag caaaagtcaa tgtcgatttg atgcatcagg catatcaacg ccaaatggcg
      781 aacgcacgcc ttggcacgca caacaccaaa cgacgtgccg aagtgcgtgg cggagcaggt
      841 aagccattta agcaaaaagg tactggtcgc gcccgacgtg gtagcatgaa gtctgcccag
      901 catgtgggtg gtggtcgcat tttcaccccc aaaccgcgtg attacagcca ggaaatgcct
      961 cgcaagatgc gccgagccgc tttgcgctcc gcgctgacgg ttgttgctaa tgatgagaaa
     1021 atcattatgc tcgataatct caacatgcct gaagcaaaaa ccgctgaatt agcaaaatca
     1081 ataaaagctc ttgctggcga caatcgcgtg ttagtgctca tcccaggaaa cagtgaagaa
     1141 aatgaaaact tcatcaaatc cgggcgaaat ctggcaaacg ccaggctgtt gcaggcacgc
     1201 tatttgaaca ttcgcgattt gctgatttca gataaagtaa tcatcccgct tgaagcatta
     1261 gatgttatca agagctattt gggataggaa ggtgaagtaa aatgactatt tcagcatttg
     1321 atatcatccg ccaaccaatc gtcactgaaa aaagcaacta tttggctggt cagcttcagc
     1381 aatatgtgtt tgaagtcgct tcgtttgcca cccgcgagca agtaaagacc gccatcgaaa
     1441 ccgcttttga tgtgaaggtt gagcgcgtca atattatcaa cgctccggcc aagtcaaccc
     1501 ggaactcacg ttcccgccgg ttgaccattc gcaagcgtgg ctacaaaaaa gccattgtta
     1561 cgttgcgcgc cggagaccgc atcccattct ttgagggagt agaataatgg cagttaaagt
     1621 atacaaaccc actagccctg gtcaacgaca caagaccagt tatacgtttg aagaaattac
     1681 aaagaccacg cccgagcgca gcttaatcta caccaagaaa aaccgcgccg gccgaaacgc
     1741 ccagggtcgc attaccgtgc gccatcgtgg tggtggttcc aagcgacaaa ttcgcatcgt
     1801 ggacttcaaa cgcaacaaac atgacatccc cgccaaagta gcggcgattg aatacgatcc
     1861 caaccgcact gcccgcctgg ctttgctcca ttatgctgat ggcgaaaaac gctatatctt
     1921 ggctcccatc ggcgtgaaag ttggcgacac cttgttttct ggcactcagg ttgaaatccg
     1981 acccggcaac aacttgcccc tggcaaacat tccggttggt acaaccatcc atgccattga
     2041 aatgcagccc ggcaagggtg cgcaaattgc ccgttcagcg ggtgtttcag cgcaactcct
     2101 ggccaaagaa ggtcgctacg cacatgtgcg catgccatct ggtgaagtcc gcttagtttt
     2161 gcaagactgc agcgccacta tcggtcaggt tggtaacgtt gagcacaaca acatcaaatt
     2221 gggcaaagcg ggacgctctc gtcatctggg acgtcgtcca gctgttcgcg ggtcggcaat
     2281 gtctccgcgc gaccatcccc atggtggtgg tgaaggcaag accggtatcg gtatggctgg
     2341 tcccaaaacc ccttggggca agcccacgct tggttacaaa acccgtcgca ataagcgcac
     2401 ggatgacatg attattcgcc accgctccaa ggctaaacgc cgctagtggt tgagagatag
     2461 aaaaggaaat aaactatgtc gcgatcattg aaaaaagggc catttattga cccgaaacta
     2521 ttgaaaaaag tcgaagatat gaa
//
LOCUS       DAOSFC010000212         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_39996_length_2527_cov_4.890372, whole genome shotgun sequence.
ACCESSION   DAOSFC010000212 DAOSFC010000000
VERSION     DAOSFC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39996_length_2527_cov_4.890372"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="PK791_05635"
     CDS             complement(<1..1673)
                     /locus_tag="PK791_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOH92827.1"
                     /translation="MTDETPRRTNFITDIIDEHNRTGRFGGRVHTRFPPEPNGYLHIG
                     HAKALVLDFGIAEDYGGITNLRYDDTNPVKEDVEYVDAIKEDIRWLGFDWGDREYYAS
                     DYFEQLYEMAIKLIKKGKAYVDELSPEETREYRGTLTEPGKNSPWRDRPIEESLDLFQ
                     RMRAGEFKDGELTLRAKIDMASPNINMRDPVMYRILHAHHHRTGDEWCIYPMYDFAHG
                     QSDSIEGITHSLCSLEYENHRPLYDWFIEELEIFAPQQIEFARLNLTHTVMSKRKLRR
                     IVEAGLVDSWIDPRMPTLAAMRRRGYPPKAIREFIDIVGVAKSENVIEVELLEHVVRD
                     ELNMTAPRRMAVLEPVKVIIDNYPEGQVEWFEVPDFPHDPERGGSRKVPFSREIYIEA
                     DDFMENPPSKYFRLSPGREVRLMNAYYIQYKDIVKDDDGNVVEIHVTYDPESRGGQSP
                     DGRKVRGTIHWVCAKHAVEAETRLYEQMFLEKDPEDIPDDQDFLDHFNKDSLKVVQAK
                     LEPALADAKVGEVYQFVRDGYFTLDHVTSTPEKPVWIRTLSLVDSWAKM"
     gene            complement(1775..>2527)
                     /locus_tag="PK791_05640"
     CDS             complement(1775..>2527)
                     /locus_tag="PK791_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92828.1"
                     /translation="MITYLEEEEIMFSCDAFGSYGSLDGVLFDDEAKDQLPFYEKEAL
                     RYYTNIVAAFSRNTLMAIDKAAVYPFKIIAPSHGLIWRKDPMRIVSLYQQWAKYATEQ
                     TEPGVTVLFGTMYRNTERAMEACLQTITNEGVPVEVFNVSTVHPSFILPSLWTKKGVL
                     IACPTYERAMFPAMVHALDIAEIKSVKNKVAGYFGSYAWSGGAQPVFESYAEKLNWEI
                     AGALEFVGCAKPDDIQTIQQIAQQVARKSKEG"
BASE COUNT          617 a          658 c          585 g          667 t
ORIGIN      
        1 tgcatcttcg cccagctgtc caccaaggac aaggtgcgaa tccagacagg tttttcaggt
       61 gtgctcgtaa cgtggtcaag tgtaaagtaa ccatcgcgca cgaactggta cacttccccg
      121 accttggcat cagccaaagc tggctcgagt ttggcttgta cgacctttaa agaatccttg
      181 ttgaagtggt ccaaaaagtc ctggtcatca ggtatatctt cggggtcttt ttccaggaac
      241 atttgctcgt agagtctggt ttcagcttca acagcatgtt tggcgcatac ccaatggata
      301 gtgccacgca ccttgcggcc atccggcgac tgtccaccgc gtgattcggg gtcataagtc
      361 acgtgaatct cgacaacatt gccgtcatcg tctttgacga tgtctttata ctgaatgtaa
      421 taagcattca tcagtcgcac ttcgcgtccg ggactcaagc ggaaatactt tgaaggcgga
      481 ttttccataa aatcgtccgc ttcaatataa atttcgcgcg agaacggcac tttgcgggaa
      541 ccgccccttt ccgggtcatg cgggaagtcg gggacctcaa accactccac ctgcccttcg
      601 gggtaattat cgatgatgac cttcaccggt tcaagcacag ccattcggcg aggcgcagtc
      661 atgtttagct catcgcgtac aacatgctcc agaagctcaa cttcaatgac gttctcgctc
      721 ttggcgacac ccacgatatc aataaattcc cggattgctt tgggcggata acccctgcga
      781 cgcattgcag ccagcgtggg catacggggg tcaatccagc tgtccaccaa gccagcttca
      841 acgattctgc gcaacttgcg cttgctcatc accgtatgtg tcaggttcag gcgggcaaat
      901 tcaatctgtt ggggtgcgaa aatctcaagt tcctcgataa accagtcata aagcggccga
      961 tggttctcat actccagcga acacaaggaa tgcgtaatgc cctcaatcga gtcgctctgc
     1021 ccatgcgcga agtcgtacat cggatatata caccattcgt caccagtgcg atgatggtga
     1081 gcatgcaaaa tgcgatacat aacggggtca cgcatgttga tattaggtga tgccatatcg
     1141 atcttagcac gcagggtcaa ctcgccatcc ttaaactcgc cagcacgcat acgctggaag
     1201 agatccaggc tttcttcaat gggtcggtca cgccagggac tgtttttacc cggttcagtc
     1261 agcgttccac gatattcgcg ggtttcttcg gggctaagtt catcaacata ggctttgccc
     1321 tttttgatca gtttgatagc catctcatag agttgctcaa agtaatctga agcgtaatac
     1381 tccctgtcgc cccaatcaaa gcccagccag cgaatatctt ccttaatagc gtcaacgtat
     1441 tcaacgtctt ctttgacggg attcgtatcg tcatagcgta agttcgtgat accgccataa
     1501 tcttcagcga tgccaaaatc aagcactaaa gctttggcat ggccgatatg taaatagccg
     1561 tttggttccg gagggaaacg ggtatgcacc ctgccaccaa agcgaccggt gcggttatgt
     1621 tcatcaatga tatcagtgat aaaatttgtt cttcttggcg tttcgtctgt cataattctc
     1681 tccttatatg gcttgtagat tataccttat ctaagcctga ttacgaaaaa agccctgaaa
     1741 gttcagggct tttgcttagc taaacactgt ggctttagcc ctcttttgat tttcttgcaa
     1801 cttgttgagc gatttgctgg atcgtttgga tgtcatcagg cttggcacag ccgacaaatt
     1861 ccaatgcccc ggcaatctcc cagttcaatt tttctgcgta gctttcaaat actggttgcg
     1921 cgccacccga ccaggcatag ctgccaaaat agcccgccac cttgtttttt acgctcttga
     1981 tttcagcgat atccagggcg tgaaccatgg cggggaacat cgcgcgttca taggtcggac
     2041 aagcaataag cacacctttt ttcgtccaca aagacggcaa gataaagctc ggatgaaccg
     2101 tgctcacgtt aaagacttcc acaggcacgc cctcgttcgt gatggtttgc aagcaagctt
     2161 ccatcgcgcg ttccgtgttg cgatacatgg tgccaaacag cacggtaacg cccggttcag
     2221 tttgttcggt agcgtacttt gcccattgct gataaagcga cacaatacgc atgggatctt
     2281 tgcgccaaat cagaccatgt gatggcgcga taatcttgaa cggatatacc gcagctttat
     2341 caatcgccat caaggtattg cggctaaacg cggcaacgat gttggtgtaa taccttaagg
     2401 cttccttctc gtagaaaggc agttgatctt tggcttcatc gtcaaaaaga acgccatcta
     2461 aactgccata ggaaccaaag gcatcacagc taaacatgat ttcttcttct tccaggtagg
     2521 taatcat
//
LOCUS       DAOSFC010000213         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_41688_length_2457_cov_4.317236, whole genome shotgun sequence.
ACCESSION   DAOSFC010000213 DAOSFC010000000
VERSION     DAOSFC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41688_length_2457_cov_4.317236"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PK791_05645"
     CDS             complement(<1..1163)
                     /locus_tag="PK791_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92829.1"
                     /translation="MTFTVRFLGQDGKCLHTGGVSLRPLSMSWSALGGCDSAEISVQG
                     DGLDIADWQGYLAKPVEIFDELGRLRWWGWLESVEKRLPGMRLGYDLSKMANRVAVVF
                     DALPPDDMLGEKQQTAWVDDAESQALYGIKERVLLGGSLTLEQAEHWRDAELQRWRLP
                     VLQAEPTSTSGNLGLVLSCKGWMHRLDWRVWQRESGVIANTVSQSGVQAVGADLQNAQ
                     IAQSFLVPRTVHLSAIQVRLRKQGLPVDKLRVDIRADQNCSPATGTLASCSLDPSELS
                     PTAYAWVRGVFPAQISLTAGNRYWFVIRRLGTLDSANHCLMALDENLSFPHGEMKLFN
                     QSEQSWQVRVPPADALFKLTVLSRIEDEIAEILQLATDFLTGSDLEASIGLRLP"
     gene            complement(1160..>2457)
                     /locus_tag="PK791_05650"
     CDS             complement(1160..>2457)
                     /locus_tag="PK791_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92830.1"
                     /translation="AIDWFKLDEGPFMPGFLGADYALKEAEAGLCHEEICLRLRGSHA
                     EMSAIIRQLESALRGALLEKQAESAYLRLYSQTLAGYVYARVIQAQLTPLPGHISSKE
                     LGSYCLKVSIVREARFVGDEVRLSIFNSGGTGDARGLTLYNHDDSHTGHDHWFSALTS
                     GLGLSEPCPMRLEFYVPEGAVSIGDFHLGSFGISEAGSFPPMTLEAEQGTGGTTIADA
                     RASNGKFQRYSWSGSGWANLASWSLSSMLVSKLASRTVLSVLRLHNLTSQTGIRLRFV
                     LRQQGVKVYESPICSLQTGKSGQVFSPMHLGAGGLPGLNYAYGLNLSLEALQSDSSAS
                     LDVDDIIFLPQKCYRFYQSLCGLEPRSSLIDDSWREQTWSLKEDQELKTHLSLGRAMS
                     LFPGLEQRFYLFMSSPAGLSAIDMTMKVRAWYRPVTVLP"
BASE COUNT          608 a          759 c          583 g          507 t
ORIGIN      
        1 ggcaagcgca agccgatgga cgcttccaaa tccgaacccg tcagaaaatc agtcgccagc
       61 tgcaagattt cagcaatttc atcttcaatc cgagacaaaa cggtgagttt gaacaaggcg
      121 tccgctggtg gcacacgaac ctgccaactt tgctcgcttt gattgaacaa tttcatctct
      181 ccatgtggaa agcttaagtt ttcatcaagc gccatcaggc aatggttcgc gctatccaac
      241 gtgcccaaac gtctgatcac aaaccaatag cggttcccgg cagtcaggct gatttgcgcg
      301 gggaatacac cgcgcaccca ggcataagcc gtgggactta gctccgatgg gtcaagactg
      361 cacgatgcca aggtgcccgt ggcgggcgaa cagttttggt cagctctgat gtccacacgc
      421 agcttatcca caggcaagcc ttgtttgcgc aatctgactt gaatcgcgct taagtgcacg
      481 gtgcggggaa ccaaaaaaga ctgcgcgatt tgcgcgtttt gcaaatccgc gcccacggct
      541 tgcacgccac tttggctgac cgtgttggca atgacacccg actcgcgttg ccacacacgc
      601 cagtccaggc gatgcatcca gcctttacag cttaagacca ggcccaaatt tccgctcgtg
      661 gaggtcggct ctgcttgcaa aaccggcaag cgccagcgtt gcagttcagc gtcgcgccag
      721 tgttcagctt gctcaagcgt gagggaccca cccagcaaaa cacgctcctt gataccatac
      781 agcgcctggc tttccgcgtc atccacccag gcggtttgtt gcttttcgcc cagcatatca
      841 tcgggtggca aggcatcaaa aacaaccgcg acgcggttcg ccattttgga taaatcatag
      901 ccaaggcgca tacccggcaa tcgcttttcg accgactcaa gccagcccca ccagcgtagg
      961 cgtcccagct catcgaaaat ttcaactggc tttgccaaat aaccctgcca gtccgcaata
     1021 tccaagccat cgccttgcac gctgatttcg gcagaatcac aaccacccaa agctgaccaa
     1081 ctcatcgaca aggggcgcag ggaaaccccg cccgtgtgca gacacttgcc atcctgccct
     1141 aaaaaacgca ccgtaaaagt catggcaaca ccgttaccgg acgataccag gcccgcactt
     1201 tcatcgtcat atcaatcgcg ctcaagccgg caggcgagct catgaacagg taaaagcgtt
     1261 gttccaggcc cggaaacagg ctcatggctc tgcccaggct gagatgggtc ttcagttcct
     1321 ggtcttcctt caggctccag gtctgttcac gccagctgtc gtcaatcagg cttgaccggg
     1381 gctcaagccc acacagcgac tgataaaagc gatagcattt ttggggcaaa aagatgatat
     1441 cgtccacgtc caaactcgcg ctgctgtcac tttgcaaagc ttccagactc aggtttaacc
     1501 cataggcgta attcaagccg ggcaaaccac ccgcgccgag atgcatgggc gagaaaacct
     1561 gtccgctttt gcctgtctgc agtgagcaaa tggggctttc gtaaactttc acaccctgtt
     1621 gccttaagac aaaacgcaga cggatacccg tttggcttgt cagattatgc aagcgaagaa
     1681 ccgacaaaac agttcgcgaa gccagcttac tcaccagcat ggatgacaaa ctccagcttg
     1741 ccaggttcgc ccagccactt ccgctccagc tataacgttg aaacttcccg tttgaagcac
     1801 gcgcgtccgc aatcgtcgtc ccgccggtgc cctgttcagc ctcaagcgtc atgggcggaa
     1861 agctgcccgc ttctgaaatc ccaaaactcc ccaaatgaaa atcgccgatg cttaccgcgc
     1921 cttccggtac ataaaactca aggcgcatcg gacatggctc actcaaaccc agtcccgaag
     1981 tcagggcgct aaaccaatgg tcatgcccgg tatggctgtc atcgtgattg taaagcgtca
     2041 acccccgcgc gtcgcctgta ccgccagagt tgaaaatcga caggcgtact tcgtcgccca
     2101 caaaccgcgc ttcccgaacg atactgactt tcaaacaata agaccccagt tcttttgagc
     2161 tgatatgtcc gggcaaaggc gtcaattgtg cctggataac gcgcgcgtaa acatagccag
     2221 ccaaagtttg gctatacaaa cgcagatacg cgctttcagc ttgcttttcc agcagagcgc
     2281 cgcgcaaagc agattccagc tgtctgatga tggcgctcat ctcagcatga ctgccacgca
     2341 ggcgcaagca aatttcttca tgacacaagc ccgcttcggc ttccttcagc gcgtaatcag
     2401 cacccaaaaa gcccggcata aaaggccctt catccagctt gaaccagtca attgcct
//
LOCUS       DAOSFC010000214         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_41957_length_2446_cov_5.232539, whole genome shotgun sequence.
ACCESSION   DAOSFC010000214 DAOSFC010000000
VERSION     DAOSFC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41957_length_2446_cov_5.232539"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1293)
                     /locus_tag="PK791_05655"
     CDS             complement(40..1293)
                     /locus_tag="PK791_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOH92831.1"
                     /translation="MEKTRTLPSHYTRFISLAIVAVLGLMGLMACLPDGQQGRPSLGD
                     MPELALPSGTPGPGQTPHSDGESVATRLAWPTFAEPNRQSVTQLPPAVSPLELDDDTS
                     VWLLLGTEFEAPNAGRTNAIHLIFIQERLSKASVVSVPGNLFVYLPGHNMQRISNAYA
                     LGGMSLLRDTLAYNFGILPDRFVLAHAEEFTWLVDDLGGIDVSVLFPIRDDCGGLPAG
                     LHHMKGEQALCYVSYNKDGDEVNRTRRQQQVLQLLFTKLVQDGRLAKLPAMYLSYQDK
                     IETDLSLLDFLQRIPLALRLGDPQRVNYFLIGWESASLWELPDHTRTKVLLPNLEALE
                     KTFAQALEAIDGASPLGEVVVTYQWQLTEAVAGTQTRQAADPQSTSLAPTRTPTPRPT
                     RTPLSPVYTPASTAIIATATPGTYP"
     gene            1504..1878
                     /locus_tag="PK791_05660"
     CDS             1504..1878
                     /locus_tag="PK791_05660"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH92832.1"
                     /translation="MDKNKLLKAIADDTRMNILTLLLRHNYCVRALANELNLSEATIS
                     QHLKVLREAGLLVGEKRGYYMHYTVERDVLQELAKEIESLVTIKTEICLPETQKDCPA
                     NEKQECHKKGECDEETKKICHG"
     gene            1904..>2446
                     /locus_tag="PK791_05665"
     CDS             1904..>2446
                     /locus_tag="PK791_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92833.1"
                     /translation="MAMVSVKNLKKTYGKFTAVDDISFEIQKGEIFGFLGPNGAGKTS
                     TINMMIGLSRPTAGEITVDGIDVRKQTKKAQSIMGIVPDESNLYNEMDGFNNLCFCAA
                     LYGMRKKEREAKALELLELFKLKDAGKRPFKAYSKGMRRKLTIAAALIHSPQVLFLDE
                     PTTGIDVESSRQIRNMIVDLK"
BASE COUNT          693 a          580 c          602 g          571 t
ORIGIN      
        1 tgcttgtttc gcgtttcggt ttcatcatat cattccacac tagggataag tccccggtgt
       61 tgcagtagca ataattgctg ttgacgcagg tgtataaacc ggcgacaacg gcgttcgggt
      121 tggcctgggc gttggcgtgc gcgtgggcgc taaagatgtt gattgcgggt ccgcagcctg
      181 acgggtttgt gtgccggcaa cagcttcggt gagctgccat tgataggtca ccaccacctc
      241 gcccaaaggt gacgcgccat caatcgcttc aagggcttgc gcgaaagttt tttccagtgc
      301 ctcgagattt ggcagcaaga ccttggtcct ggtgtggtca ggcagttccc aaagcgacgc
      361 gctttcccat ccgatcagga aatagttcac gcgttggggg tcacccaaac gcaaggctaa
      421 gggaatgcgc tgcaggaaat caagcaggct gagatctgtc tcaattttgt cctgatacga
      481 caaatacatc gccggcagtt tcgccaaacg gccatcctga actaattttg taaagagcaa
      541 ttgcaatact tgctgctggc gacgcgtgcg gttgacctca tcgccatctt tattatacga
      601 aacataacac aaggcttgtt cgcccttcat gtgatgcaag cccgctggca agccaccaca
      661 gtcatcacga atggggaaca aaacgcttac atcaatgccg cctaaatcgt caaccagcca
      721 ggtaaactct tcggcatgtg ccaaaacaaa acggtcgggc aatatgccaa aattgtaagc
      781 cagcgtgtcg cgcaaaaggg acatcccacc cagcgcgtag gcgttgctga tgcgctgcat
      841 attgtgcccc ggcaaataaa caaacagatt gcccggcacc gacaccacac tcgcttttga
      901 gagacgctcc tggataaaaa tcaggtgaat agcgttcgtg cgtccggcgt tgggggcttc
      961 aaactccgtg cccagcaaaa gccacacgct ggtgtcgtca tccagttcaa gcggactgac
     1021 cgctggaggc agctgcgtga ccgactggcg atttggttcc gcgaaagttg gccaggccaa
     1081 gcgcgtggcg acagactctc catcgctatg gggtgtctgg cctgggccag gcgttcccga
     1141 tggcaaagcc agctccggca tatcgcccag cgacggtctg ccctgttgcc catcaggcag
     1201 acaagccatc aagcccatca aaccaagcac cgcgacgatg gcaagcgaaa taaatctcgt
     1261 atagtgactt ggtaaggttc gtgttttttc catagcaaaa tcagggttgt tcacaaccct
     1321 ccaggctaac aattatagca tagctgatga tgcttgcgaa tgaagatata acaaattccc
     1381 tcattctcaa aaatcgtttt taatttagat tacctttaat atctaattag gtattgacct
     1441 aatcaaaaca atgtattaca atctaattag gtgtatgcct aattagataa caggagattt
     1501 taaatggata agaataaatt gttaaaagct attgctgatg atacccgtat gaatattttg
     1561 actttgttac tgcgccacaa ctactgtgtc cgcgcgctgg ccaacgaact caatctgtcc
     1621 gaagcgacca tttcccagca tctaaaggta ctgcgcgaag cgggcttgct tgtgggcgaa
     1681 aaacgtggct attatatgca ctacacagtt gagcgagacg ttttgcagga actggcgaaa
     1741 gagattgaat cgctggttac catcaaaacg gaaatctgtt tacccgaaac tcaaaaagac
     1801 tgtcccgcca atgaaaagca ggaatgccat aaaaaaggcg aatgtgacga ggaaacgaag
     1861 aaaatctgtc acggctaaga tataagcaaa tgagataaac agtatggcaa tggtcagtgt
     1921 caaaaatcta aaaaagacat atggcaagtt taccgctgta gatgatatct cctttgaaat
     1981 tcagaaaggc gaaatatttg gtttcctggg accaaacgga gcaggaaaaa catctaccat
     2041 caacatgatg atcggactgt cgcgacctac cgcaggcgag attacggttg atggtataga
     2101 tgtgcgaaaa cagacaaaga aagctcaatc catcatgggg attgttccag atgaaagcaa
     2161 tctctacaat gaaatggatg gatttaataa tctctgtttt tgtgctgctt tgtatgggat
     2221 gcgcaaaaaa gaacgggaag caaaagcact ggagcttttg gagctgttca agctaaaaga
     2281 tgcaggcaag cgaccgttta aagcctattc caaaggaatg cgtcgcaaac tcaccattgc
     2341 ggcggcactt atccactcgc cacaagtttt gtttctggac gaaccaacca caggaattga
     2401 cgtagagagt tccaggcaaa ttcgcaatat gatcgtggac ttgaaa
//
LOCUS       DAOSFC010000215         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_42357_length_2430_cov_4.597053, whole genome shotgun sequence.
ACCESSION   DAOSFC010000215 DAOSFC010000000
VERSION     DAOSFC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42357_length_2430_cov_4.597053"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..821)
                     /locus_tag="PK791_05670"
     CDS             complement(123..821)
                     /locus_tag="PK791_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOH92834.1"
                     /translation="MRKNKTGTLYIVATPIGHNDDITLHAIEILKKVDIVICEELRQG
                     STLLKRLKIKNNLIPLNEHNENEMVQNILVELLNGKNMALISDCGTPVFSDPGRKLLQ
                     IAYDSSVPVRPVPGTSSLMAAISICPLNLDQFTFAGFLPPQSTKRLKVLRGYSHSPHP
                     IILMDTPYRLGKLLNECGEIFGRKQTIFLACNMTQSNERNYLGTIEELLPQLENTKSE
                     FILIVDKPQRRRQR"
     gene            916..>2430
                     /gene="murJ"
                     /locus_tag="PK791_05675"
     CDS             916..>2430
                     /gene="murJ"
                     /locus_tag="PK791_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOH92835.1"
                     /translation="MKKLSRLSRVSLLLAILFAFDKILSVIRQMLISSEFGISEALDV
                     YNSANNVPDMLFMLISGGALAIAFIPILTEVLGKEGQQKAWDLFSNILNIGFLITAAL
                     AIIFSIFARPLLQYLIVPDFSTEQIDLAIPLLRLNFISTLIFSISGLVMAGLQANEHF
                     LLPALAPIFYTFGQIFGALFLAPETGFRIGSIQLPSLGLGVHGLVYGAIIGAVLHLLI
                     QVPGLIKYNYHWRPKVDFKEPYTRKVFKVMGPRLITMFLIQMVFIMQDNMASGLETGA
                     ISALMYAYWIMQIPQTLLGTSVATAVLPTLSELFNSKKFDEFKEKIERATKVMLVLTI
                     PVAVIAAVVVEPAIAVFIRGLNAEEIARVVLVTRIFLAGIIGHSLVELFARSFYAQQK
                     PYVPMLGAAVTLAIFVACGFGLIKLLPNWGIASEVGIVIANVLAYSAQAVLLYILLNR
                     RLVIVLGTEKLQTWLVFGKAVLAAVIGGALAFAVINYLPRYQHTLMAAILAGILG"
BASE COUNT          627 a          443 c          560 g          800 t
ORIGIN      
        1 aagtccaact gcttgtaaat cctgaccggt tagagctcga acatagccat aagttccatc
       61 aggcataaat gttggaaagt tcaattgctt gttgcgaacc gtaaatgtgc gggctgaagg
      121 tttcatcttt ggcgccttct ttggggtttg tcaacaatga gaatgaattc agatttggtg
      181 ttttcaagct ggggtaaaag ctcttcaatc gtgcccaggt aattacgttc gtttgactgg
      241 gtcatattgc atgctaaaaa gatggtttgt tttctgccaa aaatttcgcc acactcgttg
      301 agcagtttgc ctaagcggta tggtgtatcc atcaaaatga tgggatgagg agaatgacta
      361 taaccgcgca atactttaag acgtttggtg gattggggtg gtaagaatcc agcgaaagtg
      421 aattggtcaa ggttcaaagg acaaatggaa atggcagcca taagcgatga tgtgccagga
      481 acagggcgga caggcacaga agaatcatag gctatttgga gcaatttacg ccctgggtca
      541 gagaatactg gtgtgccaca atcggaaatc aacgccatgt ttttgccatt caataattca
      601 acgagaatgt tctgaaccat ctcattttca ttgtgttcgt tgaggggaat gagattattt
      661 ttgattttta atcgttttag cagagtggag ccttgacgca gttcttcgca aatgacaatg
      721 tcgacttttt tcaaaatctc aatagcgtgc aaggtaatat cgtcgttatg accaataggc
      781 gttgcaacga tgtatagagt tccagtttta ttttttctca tccagtgtcc ttcattcaat
      841 aacgattata aacaatatta cgttgatttg cttaatttca atttgtggca taattgtggc
      901 ttgatatgga gtgatatgaa aaaactaagt cgactttctc gcgtttctct tttgttggct
      961 attctgtttg cttttgacaa gatcctaagc gttatacgcc aaatgcttat ttcaagtgag
     1021 tttgggatat cggaagcttt ggatgtatat aactctgcca ataatgtgcc ggacatgctt
     1081 tttatgctga tatcaggtgg tgctttggcg attgccttta ttccgatttt gacagaagtt
     1141 ctgggaaaag aaggacaaca aaaggcctgg gatttgtttt cgaacatcct aaacatcggt
     1201 tttttgatta ccgcggcgct ggcgataatc ttctctattt ttgcaagacc attgctacag
     1261 tatctgattg tgcctgattt tagcacggaa caaattgacc ttgcaatccc actgttgcga
     1321 ttgaatttta tttcaacact cattttctca atcagtgggc tggtgatggc cggcttgcaa
     1381 gcaaatgagc atttcttgct tcctgcgctt gcacccatct tttacacatt tggccaaata
     1441 tttggtgctt tgtttttagc tcctgaaaca ggctttagga taggttcaat ccaattaccc
     1501 agtctcggtt taggtgtaca tggattagtt tatggcgcaa ttattggcgc agttttacac
     1561 ttattaatac aagttccggg attaataaaa tacaactatc actggcgacc gaaagttgac
     1621 tttaaagagc cctatacacg taaagtcttt aaagtaatgg ggccacgttt aatcacgatg
     1681 tttctgatac aaatggtctt tatcatgcaa gataatatgg cgtctggact ggaaacggga
     1741 gcaatttccg ctttaatgta tgcctattgg attatgcaaa taccacagac tttattaggg
     1801 acatccgtag cgactgctgt tttgcctacg ctttctgaac tcttcaacag taaaaagttt
     1861 gatgagttta aagaaaaaat agaacgcgca acaaaagtga tgttggtttt gactattcca
     1921 gtggctgtta ttgctgctgt tgtcgttgaa cctgctattg cggtctttat ccgtggatta
     1981 aatgctgaag aaattgcccg tgttgtactg gtaacacgaa tctttttggc tgggataatt
     2041 ggtcattcgc tggtggaact tttcgcgcgc agtttttatg cccaacaaaa gccttatgtg
     2101 cccatgttag gcgcggcagt taccttggct atatttgttg catgtggttt tggattgatt
     2161 aaattattgc caaattgggg aattgcatcc gaagttggta ttgtcattgc gaatgtgctc
     2221 gcgtatagtg cccaggcagt tttactttac attcttctta accggagact tgtgattgtt
     2281 ttgggtacgg aaaagcttca aacttggctt gtttttggca aagcagtctt ggctgccgtt
     2341 attggtggag cattggcttt tgcggttata aattatttgc cacgttatca gcatacgttg
     2401 atggctgcta tactggccgg catactcggt
//
LOCUS       DAOSFC010000216         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_42434_length_2427_cov_3.737774, whole genome shotgun sequence.
ACCESSION   DAOSFC010000216 DAOSFC010000000
VERSION     DAOSFC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42434_length_2427_cov_3.737774"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1053
                     /locus_tag="PK791_05680"
     CDS             244..1053
                     /locus_tag="PK791_05680"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOH92836.1"
                     /translation="MVAINLNRLAKQLQDLREMFNQPETFRAELHRFLSFYHQYSRRK
                     HEDSIPKSFMRQYDLPIQIVPQLELSLRQLAQKETEQSFAVIEELWKDDYFEARDLAC
                     FILGQQAENQTERVVSLIDSWLSEPQDRAVVASIFEKACVGLLEFAPHQFSELTKNLL
                     ASKELRQQGYGLMALSIYLPKAHHNDLPGIYGMVRPFIAHSDLQLQSRLSELVRELAK
                     QSPGEVAYLLKEVLADTEGPEIEQRLRSYLDFFEPRYAQSIQDAIKNHAKR"
     gene            1235..1975
                     /locus_tag="PK791_05685"
     CDS             1235..1975
                     /locus_tag="PK791_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOH92837.1"
                     /translation="MNQTEAPLPMPITYQEVTETSLSHTTATPSTAIVVETPDVTVEI
                     NDALPTLDSESSSVFDYPESHYIWGIVGHAQHYYLSCESSAAVDWAWFFGVNIYESTF
                     QADMPRSDNPDLGYVGDYTTRRWGQIPPYSYGVHAGPIAEGLRAYDLPALAVKGYTLD
                     EIKQKLSEDKPIIAWIIGNLEYSEPVEYVDQAGNTVIVAPYEHVVILTGYNAESIRYM
                     NNGKFFDAPTEVFLTSFGVLGNMVVIYE"
     gene            2052..>2427
                     /locus_tag="PK791_05690"
     CDS             2052..>2427
                     /locus_tag="PK791_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92838.1"
                     /translation="MKVKRRTIVYYVKKHLFHFLILIVSAVILSFLSLAPIPLLGKLT
                     DILSTKQAINISFFGLKYELDIWQCFFLIGFCILLNSAGLNFFGQSVSKFSNNIMYEI
                     RNDALGWIIQDSVKDNNSDNEGE"
BASE COUNT          683 a          501 c          554 g          689 t
ORIGIN      
        1 ccgacagtga aaatgaagag ttgatcaaga gcgaaaccaa agccaaactg ctcaacttta
       61 ccgaaaccga aggcgaaggc gtttgcttct attccggacg caaaaccaac acgcaggtct
      121 ttttcgcgaa gacttactag agttttatct ttggaactga tgaaagaggc tgacttgttc
      181 agtctctttt tttctgttta gtattaatgc aaagcataaa caagaatcag ctataattca
      241 gccatggttg ctatcaatct aaaccgctta gccaaacaat tgcaagactt gcgtgaaatg
      301 tttaaccaac cggaaacgtt tcgcgcggaa ttgcatcggt ttttatcttt ttatcatcaa
      361 tactcccgac gcaagcatga agattccatt cctaaaagct tcatgcggca gtacgatttg
      421 cctattcaga ttgttccaca gcttgaacta tctctgcgtc aacttgccca aaaggaaacc
      481 gagcaaagtt tcgcagttat tgaagaactt tggaaagatg attatttcga agcacgggat
      541 ttggcctgtt ttatcctggg tcagcaagca gaaaaccaaa ccgagcgggt agttagcctg
      601 attgatagct ggctttcaga accgcaggac agggcagtcg tggcgtcgat ttttgaaaaa
      661 gcctgtgtgg gactgctgga atttgcgccc catcagttta gcgaactgac caaaaacctg
      721 ctggcatcca aggaattgcg tcagcaaggc tatggactga tggcactgag tatttattta
      781 cctaaagcgc atcacaatga cttgccaggt atttatggca tggtgcggcc tttcatcgcg
      841 cattcagatt tacagttgca aagccgttta tcggaactgg tacgagaact ggcaaagcag
      901 tcgccgggcg aagtggcgta tctcttaaaa gaagtcctgg ctgatactga agggcctgaa
      961 atcgagcagc gcttgcgttc atacctggac tttttcgagc cgcgatatgc ccaaagtatt
     1021 caggacgcga taaaaaatca cgccaaacgc taagctactg gcaattagaa ttgacatggg
     1081 caaaaatgac ttcttcaaac tcgggtagac tttaaatatg aaagtttcca aattcatacg
     1141 tggaaaatgt ttggtattct tgcttggatt tattatgctg gtttcaatgt ctgggtgcaa
     1201 accgagcgaa tctgcagtat ctgagactcc cgaattgaat caaacagaag cgcctttgcc
     1261 catgccgatc acatatcagg aagtgacaga gacaagttta tctcacacaa ccgccacacc
     1321 gtctactgcg attgtcgttg aaacccccga tgtgactgtt gaaatcaatg atgcactgcc
     1381 aacgctggat agtgagtcga gttctgtgtt tgactatccc gaatcgcatt acatttgggg
     1441 cattgttggg cacgcgcaac actactatct cagctgtgaa tcgagcgcgg cggtggactg
     1501 ggcgtggttc tttggtgtaa acatttacga gtcaactttt caggccgata tgccccgatc
     1561 ggataacccc gatttaggtt atgtgggcga ttacaccaca cgcagatggg gtcaaattcc
     1621 accctattcc tatggtgtac acgcggggcc tatcgcggaa ggtttgcgcg cttacgattt
     1681 gcccgcgctt gctgtaaaag gctatacgct ggatgagata aagcaaaaac tctcggaaga
     1741 caaacccatc atcgcctgga taattggtaa tttagagtac agtgagccag tcgagtatgt
     1801 tgaccaggcg ggcaacacgg tgattgtcgc gccctacgag catgtggtta ttcttactgg
     1861 ctacaatgcc gagagtattc gctacatgaa caacggcaaa ttcttcgacg cgccaacaga
     1921 ggtgtttttg acttcgtttg gcgtgttggg gaacatggtc gtgatttacg agtaaaagct
     1981 aaattaattg gcgaaaaata taagcagttg gtatattttt catacattaa ttagagggag
     2041 gcacttcatc aatgaaagta aaacgtagga ctattgttta ctatgtaaag aaacatttat
     2101 ttcatttttt gatactgata gttagtgctg ttattctttc ttttttaagt cttgcaccta
     2161 ttcctttatt agggaaatta actgacattc ttagcacaaa acaagcaatt aatatctcat
     2221 tctttggact aaaatatgaa ttagatattt ggcagtgttt cttcttaatt ggcttttgta
     2281 tccttctaaa ttctgcgggt cttaatttct tcgggcagtc cgttagtaag tttagcaata
     2341 atattatgta tgagattcgc aatgatgctt taggctggat tattcaagac tcggtaaaag
     2401 acaataattc agataatgaa ggtgaat
//
LOCUS       DAOSFC010000217         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_42592_length_2421_cov_3.849535, whole genome shotgun sequence.
ACCESSION   DAOSFC010000217 DAOSFC010000000
VERSION     DAOSFC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42592_length_2421_cov_3.849535"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="PK791_05695"
     CDS             complement(<1..1374)
                     /locus_tag="PK791_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOH92839.1"
                     /translation="MKNFFNKIKKAFPKGATGVVFVGIIVLAIIGAFFVYRAVDGFIS
                     RMTIAKLPGVPVLKDPTQAPVDVNNPEPSPDLEVNFEEPVDLDTFVMPDTWDGKKRVN
                     ILIMGIDARSPDVKAPLTDSMILFTLDPVANTAAMLSIPRDLWVRIPGSGYNKINTAY
                     SIGVINQLPGGGPAMASKTVENFLGVSIDYYAQIDFKAFVDFINLIDGVKLNVTETVL
                     LDVLDTQISFDLEPGIYTLDGDLALAYVRSRAYGDGDVSRAARQQQVILAVRDRILEF
                     SMIPTLLGNANQIYDSLSRGIHTNLSMNEMIKLGLKILNDVPRDQIQHKVIDFSYVNI
                     GQGPGGESMLRPLRNRIRELRDELFTGSTFLDTLAKEEDIQTILTEEGARVSVMNASS
                     QADLGDRTVAYLRSQGVNVVEQGNVSPSTFSTITFYGAKPYTIKFLTQLTNITNPALV
                     AYAYNGGN"
     gene            complement(1440..1709)
                     /locus_tag="PK791_05700"
     CDS             complement(1440..1709)
                     /locus_tag="PK791_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92840.1"
                     /translation="MVKLQSLAYKGNDEVKMIQKELLDLLRCPVCAKEEKGTLEYFKD
                     TWLICQDCARKYPIWDDIPIMLIDEGDKWQATDKEALPVPPPQAK"
     gene            complement(1736..>2421)
                     /locus_tag="PK791_05705"
     CDS             complement(1736..>2421)
                     /locus_tag="PK791_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOH92841.1"
                     /translation="RVQNIQVGSSTFDGILIAPGEVFSMAKYMTNISLDNGYAEAIII
                     VGDQSVKGVGGGICQVSTTLFRTAFYGGYPIVERHSHAYRVGYYEQQSNGWANTNLAG
                     LDASIYLPFADLKFKNDSDYWILMDTEMGPNSLTWKFYSTSDGRRVEWSTTGITDVVE
                     PPEPIYREDPTLPRGTIKQVDWAVNGATVSVTRRVYKGDQLWFSDVINTTYMPWPDGF
                     NYGPGTEIP"
BASE COUNT          610 a          550 c          631 g          630 t
ORIGIN      
        1 gttgccaccg ttataggcat aggcgaccag cgcggggttg gtaatattcg tcagttgggt
       61 caaaaactta atcgtgtaag gtttggcgcc ataaaaagta atcgtgctga atgtgctggg
      121 gctgacgttg ccttgttcaa cgacgttgac accctgcgag cgcaggtaag caacggttct
      181 gtcgcccaaa tctgcctgcg aagacgcgtt catgaccgaa actctggcac cttcttcagt
      241 caaaatggtt tggatatctt cttctttcgc caaggtgtcc aaaaaggtac tgccagtaaa
      301 cagttcgtcg cgcagttcgc ggattcggtt gcgcaggggg cgtaacatgg actcgccacc
      361 agggccttga ccaatattga catagctgaa gtcgataact ttgtgctgaa tttgatcgcg
      421 gggaacgtca ttcaaaattt ttaagcccag ctttatcatc tcattcatac ttaaattggt
      481 atgaatgcct cttgaaagac tgtcataaat ctggttggca ttgccaagca gggttggaat
      541 catcgagaat tccaggattc tgtcgcgaac ggcaaggatg acttgttgtt gtcgcgcagc
      601 gcgactgaca tcaccatcac cataggcgcg actgcgcaca tacgccagcg cgagatcacc
      661 gtcaagggta tatatgcctg gctcaaggtc gaatgaaatt tgtgtgtcga gcacgtccaa
      721 aagcactgtt tcggtgacgt taagctttac gccgtcaata aggttaatga agtcaacaaa
      781 tgccttaaag tcaatttgcg catagtagtc aatagataca ccgagaaagt tttcgacggt
      841 tttactggcc atggctgggc caccacccgg gagttgatta atgactccaa tactataagc
      901 tgtgttgatt ttgttatagc cagaaccggg tatgcgtacc caaaggtcgc gggggataga
      961 caacatggcg gcggtattgg cgaccgggtc aagcgtgaac agaatcatgg agtcggtgag
     1021 tggggctttg acgtcggggc tgcgcgcgtc aatacccata atgagaatat tgacccgctt
     1081 tttaccgtcc caggtatcgg gcataacaaa ggtgtccagg tcaacgggct cttcaaagtt
     1141 gacttccagg tctggacttg gttctggatt attcacgtca acaggggctt gagtggggtc
     1201 ctttagcaca ggaacgccgg gcagtttggc aattgtcatg cgcgaaataa agccatccac
     1261 cgcgcgatag acgaaaaacg cgccaataat cgcgagcacg ataatgccaa caaacacgac
     1321 accagtcgcg ccttttggaa aggctttttt gattttatta aagaaatttt tcatgcaatt
     1381 ttctcgttct atgctttaat atttccgcag aaagcagtta atatgaagca gtaaattttt
     1441 tatttagctt gtgggggagg cacaggcaaa gcttctttat cagtggcttg ccatttatcg
     1501 ccttcgtcaa tgagcataat ggggatatca tcccaaatgg gatatttgcg ggcgcaatcc
     1561 tggcagatta accaggtatc tttaaaatac tcaagggtgc ctttttcttc tttggcacaa
     1621 acaggacagc gtaataaatc aagcagttct ttttgaatca tctttacctc atcattgcct
     1681 ttgtaagcta acgattgcaa ttttaccata gctgcgggga tccaaaaaaa ggcagctagg
     1741 ggatctctgt gccagggccg taattgaatc catcgggcca gggcatgtaa gtggtgttga
     1801 ttacatcgct aaaccagagc tggtcgcctt tgtaaacgcg gcgtgtaacc gataccgtgg
     1861 caccattgac cgcccagtca acctgtttga tggtaccgcg tgggagtgtg gggtcttcgc
     1921 ggtaaatggg ctcgggtggc tcaaccacat cggtgatgcc ggtggtgctc cattcaactc
     1981 ggcgaccatc gctggttgaa taaaatttcc aggtaagtga gttgggaccc atttcggtat
     2041 ccatcaaaat ccagtaatcc gagtcatttt tgaacttcag gtccgcaaag ggcaaataaa
     2101 tgctcgcgtc aagaccggcc aggttggtgt tcgcccagcc gttgctttgt tgttcgtaat
     2161 agccaacgcg gtaagcgtga gagtggcgct caacaatagg atagccacca taaaaggcgg
     2221 ttctgaaaag cgtggtgctg acctggcaaa ttcccccgcc gacgcctttt accgattggt
     2281 caccaacgat gatgatggct tcggcgtagc cgttatccag gctgatgttg gtcatgtatt
     2341 tggccatgga gaatacttct ccgggcgcga tgagaatacc atcaaaagtg cttgagccga
     2401 cttgaatatt ctggacacgg g
//
LOCUS       DAOSFC010000218         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_44022_length_2369_cov_5.344857, whole genome shotgun sequence.
ACCESSION   DAOSFC010000218 DAOSFC010000000
VERSION     DAOSFC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44022_length_2369_cov_5.344857"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK791_05710"
     CDS             complement(<1..586)
                     /locus_tag="PK791_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92842.1"
                     /translation="MALLTLFTTPKPFQDPHIITIQRNALRSWKALGDEVEIVFIGDE
                     YGMPEIAREFGLIHRPDVQVNELGTPLISSIFNIGRAENDSPFLAYVNTDIILFPDFP
                     ETLKKVANHLSKFLLVGQRYDLDITEELAFEPGWQTSLLKKAQTEGKRHARAGSDYFI
                     FPRACFKDIPDFRVGRAGWDNWMIYKARREKWTAV"
     gene            complement(590..1468)
                     /locus_tag="PK791_05715"
     CDS             complement(590..1468)
                     /locus_tag="PK791_05715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92843.1"
                     /translation="MTSIPNPKCLLCGSEKSKLYKTIESFGHPVSYYLCLDCGFVYQN
                     EAETIAKDEAFYEENYRTLYQDSEEPTAKDLRQQTLRALDQAKFLRANHYAPKNVLDI
                     GASSGLLLDTFRQEFGAGVTGIEPGNAYRAAAEKRDIQMFSSLENLLETNHNTFDLIT
                     MMHVLEHLPKPVETLREIRQSLLASNGALLLEVPNFYAHDSFELAHLSCFTPHSLEEI
                     LRLAGYEIQAFRKHGHPRSKTLDLYIGALARPIIPEHEPAPVHHERCVPLKRALAMLW
                     RKVITRLRPKDSWLGA"
     gene            complement(1490..2293)
                     /locus_tag="PK791_05720"
     CDS             complement(1490..2293)
                     /locus_tag="PK791_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOH92844.1"
                     /translation="MMETKDNQNRPVVAIIQARMSSSRLPGKALLPIAGVPMLKRVFD
                     RVQRASLVDKVVVATTSDPSDDAIEKACAEWRVACFRGSLYDVLDRYYQAAKFYNAKT
                     IVRITADCPMIDPLEIDKVLTRFFETDCDFAANRLPPPFNRTTPIGMDTEVCSFDALQ
                     RAWENAELSFEREHVMPWLYETPGRAKICVVDDPRGIGHLRFTVDTPADYALAEQIYT
                     YFGENEAFGLDELLAANAKHPEWQASLKEIQHKSYLDTDHRALNQEEKP"
BASE COUNT          573 a          572 c          612 g          612 t
ORIGIN      
        1 caaccgcggt ccacttttcg cgacgcgctt tgtaaatcat ccagttatcc cagcccgctc
       61 tgccaacgcg aaagtcgggt atgtccttga agcaagcgcg cggaaaaata aagtagtcac
      121 ttcccgcgcg ggcgtggcgt ttgccttcag tttgggcttt tttcagtaag ctggtttgcc
      181 agcctggttc aaaagctaat tcttcggtga tgtccaggtc gtaacgctga ccaaccagca
      241 ggaatttgga tagatggttg gctactttct tcagcgtttc aggaaagtca gggaaaagaa
      301 tgatatctgt gttgacgtaa gccaaaaaag gactgtcatt ttcagccctg ccgatattga
      361 aaatggaact aattaacggt gtgccaagtt cattgacttg cacatcggga cggtgaatca
      421 aaccaaattc ccgggcaatc tctggcatgc catattcatc gccgatgaac acaatctcaa
      481 cttcgtcacc aagggctttc catgatcgca aggcgttgcg ctgaatagtg atgatgtggg
      541 ggtcctgaaa gggcttgggt gttgtgaaaa gggtcaaaag ggccataggc tacgctccca
      601 accagctgtc tttgggtcgc aagcgggtga tgactttgcg ccaaagcatc gccaacgcgc
      661 gttttagggg cacgcaacgt tcgtggtgta ccggagccgg ttcgtgctcg gggataatcg
      721 ggcgtgccaa agcgccaata tacaaatcaa gcgtctttga acgcggatgg ccatgcttgc
      781 gaaaagcctg tatctcataa cccgccaggc gcaaaatttc ctcgagactg tgaggcgtga
      841 aacagctgag atgggcgagt tcgaacgaat catgggcgta aaaattaggc acttcgagca
      901 aaagcgcgcc attgctggct aaaagtgact gacgaatctc acgcaaagtt tcgactggct
      961 tgggcagatg ctccaaaaca tgcatcatcg ttatcaggtc aaacgtattg tgatttgttt
     1021 ccagcaggtt ttccagggaa ctaaacattt ggatatcccg tttttctgct gcggcacgat
     1081 aagcattgcc aggctctatg ccggttaccc cagcgccaaa ttcttggcga aaagtatcca
     1141 gcagaagacc acttgaagcg ccgatatcca aaacgttttt gggcgcgtag tggttcgcgc
     1201 gcaaaaattt agcctggtcc agcgcgcgca gagtctgttg acgcaggtct ttggcagttg
     1261 gctcttcgct gtcctgatac aaagtacgat agttttcttc gtaaaaagct tcatctttgg
     1321 cgatggtttc agcttcgttt tggtagacaa agccacaatc caggcacaga taatagctaa
     1381 cagggtgacc aaagctttca attgttttat ataatttgct cttctcactg ccgcataaga
     1441 ggcattttgg gttcggaatg gatgtcataa caataatctc tgataattcc taaggttttt
     1501 cttcttgatt tagggcacgg tggtctgtgt ctaaatagga tttgtgttgt atttctttaa
     1561 gcgacgcttg ccattcgggg tgtttggcgt tggctgcaag cagttcatca agcccaaaag
     1621 cctcgttctc gccaaagtaa gtgtaaattt gttcagccag cgcgtaatcc gcgggggtgt
     1681 ccacagtgaa gcgcaaatgg ccaatcccgc gcgggtcgtc caccacgcag attttggctc
     1741 gtcctggtgt ctcgtaaagc catggcataa catgctcgcg ctcaaaactc agttcagcat
     1801 tttcccaggc gcgttgcaaa gcgtcaaaac tgcaaacttc cgtgtccatg ccaatcggtg
     1861 tggtgcgatt gaatggcggt ggcagtcggt tagcggcaaa atcgcagtct gtttcaaaaa
     1921 agcgtgtcag cactttgtca atttcaaggg ggtctatcat cgggcaatca gcggtgatac
     1981 gcacgatggt cttggcgtta taaaacttag cggcctgata gtagcgatcc agcacatcat
     2041 acagactgcc cctaaagcaa gctacccgcc attccgcgca ggctttttcg atggcatcgt
     2101 cgctggggtc gctggtggtt gccaccacga ctttgtctac caaggaagcc cgttgcacgc
     2161 ggtcaaacac gcgcttaagc atgggcacac cagctatcgg caaaagtgcc ttgccgggca
     2221 agcggcttga actcatacgg gcttgaataa ttgccacaac gggccgattt tgattgtctt
     2281 tagtttccat cattttctac cgggtcaatg ataatcgtgg atatgctttt ttcaaaacta
     2341 tcctgggcgc caatcaaacc ttcgggcgt
//
LOCUS       DAOSFC010000219         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_44156_length_2364_cov_6.096146, whole genome shotgun sequence.
ACCESSION   DAOSFC010000219 DAOSFC010000000
VERSION     DAOSFC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44156_length_2364_cov_6.096146"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PK791_05725"
     CDS             <1..1250
                     /locus_tag="PK791_05725"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BACON domain-containing protein"
                     /protein_id="HOH92845.1"
                     /translation="AGQAVDQAAATYAGQPVAFIEWGDPYYPANRINILKESGGGRYV
                     PEMFIDSTHEWQTGVDYASIVGLVDRSQARQAGASIQANYSRDGNVVTFKATVTNSSG
                     VLLSAANNAAVHAIVYEDYQAQKTSRIGRGSAKTNISYLADGATDTYIITMEVENVVN
                     WANTHYIVLVDYKTVDTKATGKYDQPQAVIATPGDVTPPLPFYIDPEEYNFTISARDQ
                     ELPTGEFTVNLSAGKTWTAESNVEWMTIEPASGAHGDTITVSFDKTKLVEGLNKGMVV
                     VSENGSTRQRAGLVNITFVIPPPPNFKVLPVSLVYTIRHDDPPGPTAGIRISGDTPQT
                     WTAEASHNWIVLGATSGNVPGTLVVNFDRTKLAPGINEGTIIVRDGEDYHEKTVTVKI
                     TYIPEGGQEPVYNLFLPLVYIND"
     gene            1421..>2364
                     /locus_tag="PK791_05730"
     CDS             1421..>2364
                     /locus_tag="PK791_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOH92846.1"
                     /translation="MNMRNYLKTFFLILMLLLSSCGAKPDIPAILPPEPSHTLTPVPS
                     ATPTFLPSPSHTASPSPSPSPTATPEPITFKVGKNDDMFSIALYFGVSLEALKTANPQ
                     VIPNAMSIGTELIIPITPTPGVDNENEPSPGVSASTIPAPEHVSTYKSYCYRDASKAV
                     YCFSLIENDSDEALENVSLLITLSDETGKTAQTIATTLINVIPPHSSLPAASYFLPEW
                     GETLTVSAELDFQLPYGEGETRYLETEALEPKVDYLYNKKAARVQGLISFENKNTVFM
                     SLNIVAVAYDENGAVLGMRRNGPLESSSTSANVPYSITV"
BASE COUNT          663 a          602 c          509 g          590 t
ORIGIN      
        1 ctgccggtca ggcagtagat caagctgcag caacttatgc tggacaacct gtcgccttta
       61 ttgagtgggg cgatccctat taccccgcca accgtataaa tattttaaaa gaaagcggtg
      121 gtggtcgata tgttcccgaa atgttcattg acagtaccca tgaatggcaa accggtgtcg
      181 attatgccag tattgttggt cttgttgacc gttcacaggc gcgtcaagct ggtgcatcca
      241 tccaggcgaa ctattctcgt gatggcaatg ttgttacctt taaagcaacg gtaacaaact
      301 caagcggtgt attgctctcc gctgcaaaca atgccgcggt acatgccatc gtttatgaag
      361 attatcaagc tcaaaagacg agccgcatcg gacgaggttc tgccaaaaca aatatcagct
      421 atttagctga tggagctaca gatacctata tcattaccat ggaagtggaa aatgtggtca
      481 actgggctaa cacccactat attgtcttgg ttgactataa aacagtagat acaaaagcca
      541 ctggaaagta tgaccaaccg caagcagtaa tcgccacccc tggcgatgtc acaccgccac
      601 tgccgttcta catcgaccct gaagaataca actttaccat cagcgcgcgt gaccaggaac
      661 ttcccactgg tgaattcact gtaaatttga gtgctggtaa gacttggaca gcagaatcaa
      721 atgtggaatg gatgaccatt gagccagcct ctggtgctca cggcgatacc atcaccgtaa
      781 gctttgataa aaccaaactg gttgaagggc ttaacaaggg catggttgtt gtatctgaaa
      841 atggcagcac acgacaacgt gcgggcctgg tgaacatcac ctttgttatc ccacctccac
      901 ctaattttaa agttttacct gtatcgcttg tttataccat ccgccatgat gacccacccg
      961 ggccaaccgc aggcatacgc atcagtggtg acacacccca aacctggaca gccgaagcca
     1021 gccataattg gattgtactg ggtgctacca gtggcaacgt acccggaacg ctcgtagtga
     1081 attttgaccg aactaaactt gctcctggca taaacgaagg tactatcatc gttagagatg
     1141 gcgaagatta ccacgaaaag actgtgaccg taaaaataac ttatattcct gaaggtgggc
     1201 aggaacccgt ttataatttg tttttgcctc tggtctatat caatgactaa tcgccgttga
     1261 taatggacat ttgaaaccaa cagcctcgtc aaaatgacgg ggctgtttta tttgcgaata
     1321 aaattactca aagtttatat cctatatctc atttgccttt cccgcatata taatcaactg
     1381 tgccaaatag caacttgcac gaaatgagta taataagcta atgaacatgc gtaattattt
     1441 aaagactttt ttcctgattt tgatgcttct tttgagttcc tgtggggcca aaccggacat
     1501 ccctgctatc ctgccaccag aaccgagcca tacactcacg cctgtgccca gtgccacgcc
     1561 aactttcctg ccaagtccca gccataccgc cagtcccagc ccttcgccct cgcccaccgc
     1621 aacacctgaa ccgattacat tcaaagttgg caaaaatgac gatatgttca gcatcgcgct
     1681 ttattttggg gtcagccttg aagccctgaa aactgcaaat ccccaggtta tacccaacgc
     1741 gatgtccatc ggcactgagt taatcatccc cattacgcct acaccgggcg tggacaacga
     1801 gaatgagcca agtccaggcg tatctgcctc aacaatcccc gcgccagagc atgttagtac
     1861 ctacaaaagc tattgttatc gtgatgccag caaggctgtt tactgcttta gcctgattga
     1921 aaatgacagt gatgaagcgt tggaaaacgt ttctcttttg ataacgctaa gtgacgaaac
     1981 tggcaaaacg gcccaaacca tcgccacgac cttaatcaac gttattccac cccacagcag
     2041 tttacctgcc gccagttatt ttttgcccga gtggggagaa acgctgaccg tatctgccga
     2101 acttgatttt caactgccct acggcgaagg tgaaacacgt tacctggaaa ccgaagcact
     2161 cgaaccaaaa gttgactatc tctacaataa aaaagcggct agagtgcaag gtttgattag
     2221 ttttgaaaac aaaaacaccg tttttatgtc tctgaatatc gtcgcggtgg cttatgacga
     2281 aaatggcgcg gttttgggaa tgcgtcgcaa tggacccctt gagagtagca gcacatccgc
     2341 caacgtgccc tattcaatta ccgt
//
LOCUS       DAOSFC010000220         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_44252_length_2361_cov_2.662619, whole genome shotgun sequence.
ACCESSION   DAOSFC010000220 DAOSFC010000000
VERSION     DAOSFC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44252_length_2361_cov_2.662619"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PK791_05735"
     CDS             <1..189
                     /locus_tag="PK791_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOH92847.1"
                     /translation="KGMPVSTTHTKTTAIMGVGASRRVRSVNWAIVKEMVLAWLLTFP
                     GCGLLGYLVTKLLMLILV"
     gene            204..824
                     /locus_tag="PK791_05740"
     CDS             204..824
                     /locus_tag="PK791_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOH92848.1"
                     /translation="MAKKDNNYFKMFMDIAADAITAATQLKETINHYDPQKIHEQLVV
                     MHEVEHAADIKKHEMMEKLIAEFITPIEREDIITLSWALDDVLDYIEEIYQILFMHNV
                     QTMRNEALVFGDLIYQSAVALKSCFDEFEAFKRSKILPKRLIEVNSIEEKADKLYIEA
                     VRRLHLDESDSSKIHTWSVLFLQLERTIDRIEQVADTIELVIMKNS"
     gene            980..1055
                     /locus_tag="PK791_05745"
     tRNA            980..1055
                     /locus_tag="PK791_05745"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1014..1016,aa:Glu,seq:ctc)
     gene            1114..2196
                     /locus_tag="PK791_05750"
     CDS             1114..2196
                     /locus_tag="PK791_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH92849.1"
                     /translation="MNKYPRYRIRRTILNGIGKGLLSLLTDVNIEGVENIPKTGPVIL
                     AGNHVESLESAFQMFYTKRIVEPIGAGDIPFDKGLDKFVHLYGFIAIDRGTVDRAALR
                     EAQSVLEQGAALGIFPEGGTWRPAKMPAQIGVALLSERGKSPVVPIGYSGFIGAIKAA
                     LNFKRPKLTMRIGKPIPALVIDPNGAPVKEQMQAYADKVMDEVHALLDEAEHNIMPER
                     ENFQFSIHREKDGQKIQDLEPIQNGEAFVSLVNTKVILDTLHTSLKLPTQPFYPGQEN
                     ISANSFIKAINATLAYLKEKPAFFTYRMGQETGEQIHGVLEQLKQLVIESKELGQDLI
                     LNGYVESFYLNGEERQTHHIFRILAD"
BASE COUNT          674 a          512 c          530 g          645 t
ORIGIN      
        1 aaaggcatgc cagtctccac aacgcacacc aaaacaaccg ccatcatggg cgttggcgca
       61 tcaaggcgtg ttcggtcggt gaattgggcg atcgttaaag aaatggtttt agcctggctg
      121 cttacattcc caggctgcgg attattgggc tatttagtga caaaattgct catgctcatc
      181 ttagtttaat gtaaaggaat aacatggcaa aaaaagacaa caattacttc aagatgttta
      241 tggatatagc ggcagacgcg attaccgctg cgacacaact caaagagaca atcaaccatt
      301 acgatcccca aaaaatccat gagcaattgg ttgtgatgca cgaagttgaa catgccgcgg
      361 atataaaaaa gcatgagatg atggaaaagc taatcgccga gttcatcaca ccaattgagc
      421 gcgaagatat catcactttg tcgtgggcgc tggacgacgt gttggactat attgaggaaa
      481 tctatcagat tctgttcatg cacaatgttc aaaccatgcg caatgaagcg cttgtatttg
      541 gtgatttgat ttatcagagt gctgtcgcgt taaaatcttg ctttgatgaa tttgaagcct
      601 tcaaacgttc taaaattttg cctaaacgtc ttattgaagt gaactcgatc gaagaaaaag
      661 ctgacaaact ttatattgaa gcagtacgcc gcttgcatct tgatgagtcg gattcgtcga
      721 aaatccacac ctggtcggtt ttgttcctgc agcttgaacg caccattgac cgcattgaac
      781 aggttgctga tacaatcgaa ctggtaatta tgaagaattc ctaaactatc ttagtttatt
      841 caagcccctc tgtgattaga ggggcttttt aatggagtgt atgatttcga gggtggagca
      901 ttttgctcac catgaacaac ggaaaagtgg gcaaataatt gacgcgtttt gttctccgtg
      961 atattatact gttcaccttg cccccatcgt ctagcggcct aggacgtggc cctctcaagg
     1021 ccaaaaccgg gattcgaatt cccgtggggg caccatgttc aagtggagag cgtaactaat
     1081 gctccccatt tttgttaagt aaatgtgaga aaaatgaaca aatacccccg ttatcgcatt
     1141 cgtcgtacca ttcttaatgg tattggcaaa ggtctactaa gccttttaac agacgtgaac
     1201 attgaaggcg ttgaaaacat tcccaaaaca ggacctgtta ttcttgctgg caatcatgtc
     1261 gaaagtcttg aatctgcttt tcagatgttt tatacaaaac gtatcgttga acctattggt
     1321 gccggtgata ttccctttga caaaggcttg gataaatttg tccatcttta tggttttatc
     1381 gccattgacc gaggaactgt tgatcgagct gccctgcgcg aagcacaatc tgtgctggaa
     1441 cagggagctg ctttgggcat tttcccagag ggtggcacct ggcgacctgc caagatgccc
     1501 gcgcaaatcg gtgtggcttt gcttagcgaa cgtggaaagt ctcctgttgt gccgattggc
     1561 tatagtggct ttataggtgc catcaaggct gctttgaact ttaaacgccc aaaactaacg
     1621 atgcgcatag gcaaacctat cccagctttg gtcattgacc caaatggcgc gcctgttaaa
     1681 gaacaaatgc aagcttacgc ggataaggtt atggatgaag tgcacgcgct cttggacgaa
     1741 gcggaacaca atatcatgcc cgaacgcgaa aactttcagt ttagcattca ccgtgaaaaa
     1801 gatggtcaaa aaattcagga cttagagccc attcagaatg gtgaagcatt tgtaagttta
     1861 gttaatacaa aagtcattct tgatacactc cataccagtc tgaaattacc aacccagccc
     1921 ttttacccag ggcaggaaaa catttcagct aacagcttca ttaaagccat caatgcgacc
     1981 ttagcgtatc tcaaggaaaa gcccgcgttt ttcacctacc gcatggggca ggaaacgggc
     2041 gagcaaatcc atggtgtttt ggagcagcta aaacaattgg ttattgaaag taaagagttg
     2101 ggacaagacc tcattctgaa tggctacgtt gaatcttttt atctgaatgg cgaagaacgc
     2161 caaacgcacc atattttcag gattttggct gactaaccag cgagcatttc ctaaacagcg
     2221 aaaagaacta tgcgggcttt ggcccgcttt ttttaatatc tttcacgttc aattgcacat
     2281 tgcgcatgcc catatactca ttgatttcaa tcgtgtaggc gatatcgata aagttcggta
     2341 tatcacttgc ccaatgtcct t
//
LOCUS       DAOSFC010000221         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_44672_length_2346_cov_5.426015, whole genome shotgun sequence.
ACCESSION   DAOSFC010000221 DAOSFC010000000
VERSION     DAOSFC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44672_length_2346_cov_5.426015"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="PK791_05755"
     CDS             <1..1601
                     /locus_tag="PK791_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOH92850.1"
                     /translation="ATYTRRVGFDKMLSIGNQSDLDFADLVTYLAEDDVTSCITLYVE
                     GIKDGARFMEAARNCDKPIIALKAGTSAHGAAAAASHTGSLAGAAKVYEAAFKQVGVT
                     QADDLNDLFDRTLALSLQPRMQGDNLLILTNGGGVGVLATDAAEKYGLPLKFAPPEVQ
                     AELKKHMPDFGSAKNPVDITGGAGLEGYYEGVKYSYAHPWVDGMAILYCETAVTNPLE
                     IAEAIYKALQDSGTTGKPVTVSFVGGERCETATAWLIERGIPTYNAPDIAVKALATLR
                     QDQLMSETSHSAPYKPTDINPEKAREIIAGARAKGRDALTEVEAKHVFAAYGLPVTPT
                     ELARTEEEAIKLANEIGFPIVMKIVSPDILHKSDAGAVKVNIKNDDEVREAWKVIMEN
                     SLKYKADAEIDGMAVQEMAPWSTEVIVGSVNDATFGPTVMFGLGGIFVEVLKDVTFRV
                     APIDEAEALRMQNEIKSAQILHGARGESPRDMAKLASILAHYSYMIYDLKDEIAESDA
                     NPCLVYEEGKGVAVVDARIILTKK"
     gene            1730..>2346
                     /locus_tag="PK791_05760"
     CDS             1730..>2346
                     /locus_tag="PK791_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOH92851.1"
                     /translation="MKKEKKSYLIDMDGVLVHGSVPIPGAAEFIQKLIEEKRKFLILT
                     NNPLYTTRDLAHRFQLSGLDISEDRIYTSAMATASFLHRQRPNGKAFVIGETGLTEAI
                     HAIGYVQTSVEPDYVVLGETNTYSFEEITKAIRLIMGGARFIATNPDITGPTESGIVP
                     ATGSMAALIEKATGKAPLFIGKPNPLMMRSALNYINAHSEESIMIG"
BASE COUNT          596 a          689 c          574 g          487 t
ORIGIN      
        1 ccgccacata cacccgccgt gtcggttttg acaagatgct ctccatcggc aaccagtctg
       61 accttgactt tgcagacctg gtcacctacc ttgccgaaga cgacgtgacg tcttgtatca
      121 ccctgtacgt tgaaggcatt aaagacggcg cgcgctttat ggaagctgcc cgcaactgtg
      181 ataaacccat tatcgcgctc aaagcgggaa catccgcgca tggcgccgcc gcggctgcct
      241 cacataccgg ctcattggct ggcgcggcaa aggtttacga agcggctttc aaacaggttg
      301 gcgttaccca ggcggatgac ctgaacgatc tctttgaccg caccttagcc ctttcgctgc
      361 aaccccgcat gcaaggcgac aacctgctca tcctcaccaa cggtggcggt gttggcgttt
      421 tggcaacgga cgcggctgaa aaatacggct tgcccctcaa gtttgcccca cccgaggttc
      481 aggcagaact caagaagcat atgcccgact ttggctccgc caagaacccg gttgacatta
      541 ccggtggcgc tggcctggaa ggctactacg aaggcgttaa atactcctac gcccacccct
      601 gggttgacgg catggcaatc ctgtactgcg aaaccgccgt caccaatccc ctggaaattg
      661 cggaagcgat ctacaaagcc ctgcaagatt ccggcacaac cggcaaaccc gtaaccgtct
      721 cctttgttgg tggcgaacgc tgcgaaaccg cgaccgcgtg gctcattgaa agaggcattc
      781 caacctataa cgcgcctgat atcgccgtga aagcactcgc aacactgcgc caggaccaac
      841 tgatgagcga aaccagccac agcgcgccct acaaacccac cgacatcaac cccgaaaaag
      901 cccgcgaaat catcgctggt gcccgcgcca aaggccgcga cgcgctgacc gaagtggaag
      961 ccaaacacgt ctttgccgca tacggtctgc cagttacccc cacggaactt gccaggaccg
     1021 aagaagaagc cattaaactc gccaacgaga ttggtttccc catcgtgatg aagattgtct
     1081 cccccgatat cctgcacaaa tccgatgccg gcgcggttaa ggtgaacatc aaaaatgacg
     1141 acgaagtgcg cgaagcctgg aaagtcatca tggaaaactc tcttaaatac aaagccgacg
     1201 ctgaaatcga cggcatggca gtgcaagaga tggctccctg gtcaaccgag gttatcgttg
     1261 gttcagtcaa cgacgctact tttggcccaa ccgtcatgtt cggtttaggt ggtatcttcg
     1321 tggaagtgct caaagacgtc accttccgcg ttgcgcctat cgacgaagcc gaagccttgc
     1381 gtatgcagaa cgaaatcaag agtgcccaaa ttctgcacgg tgcccgtggt gaatccccac
     1441 gcgatatggc gaaactggcc agcatcttgg cgcactactc ctacatgatt tacgacttga
     1501 aagacgaaat cgccgaatcc gacgccaacc cctgcctggt ttacgaagaa ggcaagggcg
     1561 tagccgttgt tgacgcgcgc attattctta ccaagaagta atcactgttt aggttcgaaa
     1621 aacaagagca ctgttggtag aatcaacagt gctcttttat ttgcaaattt tttacaagtc
     1681 ctttaaactg ctagtgaaca atgatattat gatatatgat agaggtaata tgaaaaaaga
     1741 aaagaaaagt tacttgattg atatggacgg cgtcctggtg catggttctg tacccatccc
     1801 tggcgctgct gaatttatcc aaaagctgat tgaggaaaaa cgcaagtttc tcatcctgac
     1861 caacaatccg ctttacacca cccgcgacct ggcgcaccgt tttcagcttt ccggactgga
     1921 tatttcggaa gaccgcatct acaccagcgc catggcaacg gcctcctttt tacatcgcca
     1981 gcgacccaac ggcaaggcct tcgtcattgg cgaaacaggg ctgaccgagg caattcacgc
     2041 cattggctac gtgcaaacat cggttgaacc ggattacgtc gtgctgggcg aaaccaacac
     2101 ctatagcttt gaagaaataa ccaaagccat tcggctcatc atgggtggcg cgcgctttat
     2161 tgccaccaac cccgatatca ctggcccaac cgagtcgggc atcgtccccg cgacgggttc
     2221 gatggcagcg ctcatcgaaa aagccaccgg caaggcgccg cttttcatcg gcaagcccaa
     2281 cccgctgatg atgcgctccg cgctcaacta cattaacgct cactctgaag agtcgattat
     2341 gattgg
//
LOCUS       DAOSFC010000222         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_44675_length_2346_cov_4.314710, whole genome shotgun sequence.
ACCESSION   DAOSFC010000222 DAOSFC010000000
VERSION     DAOSFC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44675_length_2346_cov_4.314710"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..>2346
                     /gene="nifJ"
                     /locus_tag="PK791_05765"
     CDS             200..>2346
                     /gene="nifJ"
                     /locus_tag="PK791_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOH92852.1"
                     /translation="MKRDMVMTDANEATAHVAYRLSEVIAIYPITPSSNMGEFADQWA
                     AERVPNLWGTIPSVIEMQSEGGAAGAVHGALQAGALTTTFTASQGLLLMIPNMYKIAG
                     ELTPAVFHVSARSIAAQALSIFGDHQDVMAVRQTGWGLIASNSPQEAQDTAAIATAAT
                     LRARVPFVHFFDGFRTSHEINNVNRISDDELRELINEDMIAEHRARALRPEKPFIRGT
                     AQNPDVYFQGRETVNRYYDEAVRIVQEEMDRFADITGRQYHVMEYYGSEKADRVVVIM
                     GSGAETAAEVANYLNTHNETVGVVVVRLYRPFPVDQFIDLLPATVKKIAVLDRTKEPG
                     AIGEPLYQDVVNALNVAVDEGRLACGTMPKVIGGRYGLSSKDFTPAMVKGVFDELAKD
                     EPKARFTVGIIDDVTNLSIDYDPSFEVLSDKVIQAMFFGLGSDGTVGANKNSIKIIGD
                     QTENFAQGYFEYDSKKSGGVTVSHLRFGPEPIKAPYFISEGQASFVACHQLSFVDRYD
                     MIKYSRPGSVFLLNTIYSPEEVWDTLPRDMQKDMIDKNIRFYVIDAYKVAEATGMGNR
                     INTIMQTCFFAISGVLPRDEAIAHIKTAIEETYGEKGESVVKRNFAAVDGSLANLHKV
                     DYPKEITSDIVRRPAVPEEAPVFVKEVLSMMIDRKGDEIPVSMMPDDGTYPSATTQWE
                     KRDIALEIPIWNPDLCIQCGKCSMVCPHAAIRT"
BASE COUNT          604 a          685 c          541 g          516 t
ORIGIN      
        1 aaaaaaaggg gagttcttca ttcgcaacag acatgattta aaacaaactg aaccgtgcta
       61 ttcatccata aaagtgaaac cttattcacc acagaatgca ctttctgcgt tacaattgtg
      121 aagacgccat ccagcaatgg gaggtgattc aatgcgtata tttaaaacgg tattaccgat
      181 aaaaaaggag ctaaaacata tgaaacgaga catggtcatg accgacgcca atgaggcaac
      241 cgcccacgtc gcttatcgct tgagcgaagt gattgcgatt tacccaatca cccccagctc
      301 aaacatgggc gaatttgcgg accaatgggc agcagaacgc gtgcccaacc tttggggcac
      361 aatcccatcg gtcatcgaaa tgcagtccga aggtggcgcg gctggcgcgg tgcatggcgc
      421 attgcaagcc ggcgcactca ccacaacctt taccgcctcc caaggccttt tactgatgat
      481 tccaaatatg tacaagattg ccggcgaact gacacccgcg gtgttccatg tttccgcgcg
      541 ctcaatcgcc gcccaggcac tttccatttt tggcgaccat caggacgtta tggcagtgcg
      601 ccaaaccggt tggggcttga ttgccagcaa ctcaccccag gaagcccagg ataccgccgc
      661 catcgctacc gccgcgacct tgcgcgcccg tgtgcctttt gtgcactttt ttgatggctt
      721 ccgcacatcg cacgaaatca acaacgtcaa tcgcatcagc gacgatgaac tccgagagct
      781 catcaacgaa gatatgattg ccgaacaccg tgcccgcgcg ctaagaccag aaaaaccctt
      841 cattcgtggc accgcccaaa accctgacgt ttacttccag ggtcgcgaaa ccgtcaaccg
      901 ctattacgac gaagccgtgc gcatcgttca ggaagaaatg gaccgcttcg cggacatcac
      961 cggtcgccaa taccacgtta tggaatacta cggttccgaa aaagctgacc gtgtggtggt
     1021 tattatgggc tctggcgctg aaaccgccgc tgaagttgcc aactatctca acacccacaa
     1081 cgaaaccgtt ggtgtggttg ttgtacgctt gtatcgacct ttccctgtgg atcagttcat
     1141 cgacttactg cctgccacag tcaagaaaat tgctgtgctt gaccgcacca aagaacccgg
     1201 cgccatcggc gagccgcttt atcaagacgt ggtcaacgcg cttaacgtgg ctgttgacga
     1261 aggtcgcttg gcttgtggca ccatgcccaa agtcatcggc ggacgctacg gtctctcatc
     1321 caaagacttt acacccgcga tggttaaggg tgtctttgat gaactcgcca aagacgaacc
     1381 caaagcccgc ttcaccgtgg gtattatcga cgatgtcacc aacctaagca tcgattacga
     1441 tcccagcttt gaagttttga gtgacaaagt tatccaggcc atgttcttcg gtcttggttc
     1501 cgatggtacc gttggtgcca acaaaaactc aatcaaaatc atcggtgatc aaaccgaaaa
     1561 cttcgcccag ggttacttcg agtacgactc caagaaatcc ggtggtgtca ccgtttcgca
     1621 cttgcgcttc ggcccagaac ccatcaaagc gccatacttc atttccgaag gtcaggccag
     1681 ctttgtggcg tgccatcagc ttagctttgt tgaccgctat gacatgatta aatactcacg
     1741 ccctggttca gtcttcttgc tcaacaccat ctacagcccc gaagaagtct gggataccct
     1801 gccccgtgat atgcaaaaag acatgattga caagaacatt cgcttctatg tcatcgacgc
     1861 atacaaagtc gccgaagcga ccggtatggg taaccgcatc aataccatca tgcagacctg
     1921 tttcttcgcc atttccggcg ttttgccccg cgacgaagcc atcgcccaca tcaaaaccgc
     1981 cattgaagaa acttacggcg aaaagggcga atccgttgtt aaacgcaact ttgcagctgt
     2041 tgacggttca ctggcaaacc tccacaaagt ggactatccc aaagaaatca ccagcgatat
     2101 cgtccgccgc ccggcagtgc ccgaagaagc tcccgtcttc gtcaaggaag tcctttccat
     2161 gatgattgac cgcaagggcg acgaaatccc cgtcagcatg atgcccgatg atggcaccta
     2221 cccctcagcc acaacccagt gggaaaaacg cgatatcgcg cttgaaattc ccatttggaa
     2281 ccccgacctg tgtatccagt gtggcaaatg ctccatggtt tgtccccatg ccgcaatccg
     2341 caccaa
//
LOCUS       DAOSFC010000223         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_44742_length_2344_cov_3.627785, whole genome shotgun sequence.
ACCESSION   DAOSFC010000223 DAOSFC010000000
VERSION     DAOSFC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44742_length_2344_cov_3.627785"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PK791_05770"
     CDS             complement(<1..496)
                     /locus_tag="PK791_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOH92853.1"
                     /translation="MTTLLERFLRYTKVDTQSQHDAESYPSTAKQLDLARLLEKELRE
                     LGMSEVELDQYGYVTATLPANIDHDCPVVGFIAHMDTSPDASGENVKARVIENYNGKD
                     IILNEAENIILSPDVFPALLKYKGESIVVTDGTTLLGADDKAGIAAIMDAMRHLIANP
                     EIKHG"
     gene            633..1472
                     /locus_tag="PK791_05775"
     CDS             633..1472
                     /locus_tag="PK791_05775"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HOH92854.1"
                     /translation="MNTKTRKIIVAIIVVLVIAFLAFALPRLLGMVKTKATVEPDILS
                     GLGGKASYEIVNIYPHDSDCYTQGLVYENGLLYESCGLYGKSRLRVAKLEDGETLREI
                     EIEDKYFAEGLALLDNQLTMLTWQEGTAFIFQKDDFTQTGTFNYSTEGWGLTTDGNAL
                     ILSDGSNTLYWMDPKSGLVVNEVHVTLEGNPMSMLNELEFVNGEILANVYMTDTILRI
                     DPISGEVLTQIDLSGLMPDANKAKLGEVLNGIAWDNKTGRLFVTGKNWDVLYEIQLVP
                     AIP"
     gene            complement(1478..>2344)
                     /locus_tag="PK791_05780"
     CDS             complement(1478..>2344)
                     /locus_tag="PK791_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOH92855.1"
                     /translation="GLLSRINPDYPLDWLKPFEIMGFDLQGIIKTDEKFDPRFFVAYT
                     PPNKTSYENALTHFAERQIPFPQELLNYSANTRRYCSKTDYMPYTFRVTDIPRPYLEA
                     NAAHICPVDYISHKILPSVLKAGMIQTLSMRATDCYMNVSFWEDMRNLIADLSIFMLS
                     EDQALKLFQGRSMDLWDIAHVLASYGPEYIIINTKDGATWVYDREGNRRFIVPAWPIR
                     LADPTGGLNAFDGGFLYNYHESYDVVHAAVCGNLSMAFCMEGSGPNYLLETLPALRDA
                     RLHALSQQIIYL"
BASE COUNT          539 a          471 c          685 g          649 t
ORIGIN      
        1 ggccatgttt gatttcgggg ttggcgatga gatggcgcat agcatccatg atggccgcga
       61 tgccggcttt atcgtccgcg ccaagtaagg tggtgccatc ggtaacgacg atgctctcgc
      121 ctttgtattt taataaggca gggaaaacgt ccggcgacag aatgatgttt tcggcttcgt
      181 tcaggatgat gtctttgcca ttgtaattct caatgacgcg cgctttgacg ttctcgccag
      241 acgcgtccgg gctggtgtcc atgtgggcga tgaagccaac aaccgggcag tcgtggtcaa
      301 tgttggcggg taaggtagct gttacatagc cgtattggtc gagttcaacc tcgctcatgc
      361 ctaattcccg cagttctttt tccaagagac gagctaggtc aagctgtttg gcggtgctgg
      421 gatagctttc ggcgtcgtgt tgtgattgtg tgtcaacttt ggtgtagcgt aaaaagcgtt
      481 cgagtagggt ggtcatggtg tctccttttt ctgttccttt tttgaatttt atcatgatga
      541 cggcttggct ggcttttcat gagcagtttt gctgatgttt ggtagcgtga tgatggcggt
      601 gtttctttcc atgctggtgg taaaatttaa gcatgaatac taagaccagg aaaattattg
      661 tcgcgattat tgtggtgctg gtgattgcct ttttggcttt tgccttgcca cgcttgttag
      721 gcatggtaaa gacgaaagca actgttgagc cggatatttt gtcgggcctg ggcggaaaag
      781 ccagctacga aattgtgaat atatatccgc atgattctga ttgttacact cagggtttgg
      841 tttacgaaaa tggcttgctt tatgaaagtt gtggcttgta tggcaagtcg cgtttgcgcg
      901 tagccaaact tgaggatggg gaaacactgc gcgaaattga gatagaagat aagtattttg
      961 ccgaaggttt ggctttgctt gacaatcagc taaccatgct gacctggcag gaaggcacgg
     1021 catttatctt tcaaaaagac gattttacgc aaaccggcac gtttaattac agtacggaag
     1081 gctgggggtt aacaactgat ggcaatgcgt taatcctgtc tgatggttca aacacgcttt
     1141 actggatgga ccccaaaagt gggttagtgg tgaatgaagt gcatgttacg ctggaaggta
     1201 acccgatgag catgctgaac gagctggagt ttgtcaacgg ggagattcta gccaacgttt
     1261 acatgaccga cacgattttg cgcattgacc cgatcagcgg ggaagtgctg acccaaattg
     1321 atttatcagg attaatgcct gatgccaaca aagccaaatt gggcgaggtg cttaatggca
     1381 tcgcttggga taataaaacg gggcgtttgt ttgttaccgg taaaaactgg gacgtgctgt
     1441 acgagattca gcttgtgccg gcaattcctt agatagttta gaggtagatg atctgttgcg
     1501 agagcgcgtg aaggcgggca tcgcgcagcg cgggcagggt ttccagcagg taattcggtc
     1561 cgctgccttc catgcagaat gccatactca ggttgccaca aaccgctgcg tgcacaacat
     1621 cgtagctttc gtggtaattg tataaaaacc caccatcaaa cgcattcaga ccgccagtag
     1681 ggtcagccag tcttatcggc caggctggca cgataaagcg cctgtttcct tcgcggtcat
     1741 agacccaggt ggcgccatct ttggtgttga tgatgatgta ttcgggaccg taactggcta
     1801 aaacatgggc gatatcccat aaatccatgc tgcgtccctg gaataatttg agcgcctggt
     1861 cttcgctaag cataaaaatg cttaggtcgg cgatgagatt gcgcatatct tcccaaaagc
     1921 tgacgttcat gtagcaatcg gtggcgcgca tggacagggt ttgaatcatg cccgctttga
     1981 gcacagaagg cagaatttta tgtgaaatat agtcaacggg gcagatgtgg gcggcgttgg
     2041 cttccaggta cgggcgcggg atgtctgtta cgcgaaaggt gtaaggcata taatcggttt
     2101 tgctgcaata gcgacgtgtg ttggctgagt agttgagaag ttcctggggg aaggggattt
     2161 gccgttctgc gaaatgggtg agcgcgttct cgtaactggt tttgttgggt ggggtgtagg
     2221 cgacaaaaaa gcgtggatca aatttttcat ctgttttgat gatgccttgc aggtcaaacc
     2281 ccatgatttc gaagggttta agccagtcca gggggtagtc ggggttgatg cggctaagca
     2341 aacc
//
LOCUS       DAOSFC010000224         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_45000_length_2336_cov_5.440158, whole genome shotgun sequence.
ACCESSION   DAOSFC010000224 DAOSFC010000000
VERSION     DAOSFC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45000_length_2336_cov_5.440158"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1121)
                     /gene="argF"
                     /locus_tag="PK791_05785"
     CDS             complement(198..1121)
                     /gene="argF"
                     /locus_tag="PK791_05785"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOH92856.1"
                     /translation="MKKDFLDITDFSKEELLGFLDLATELKKEYFAGGNEPVLKGKNL
                     AMIFQKPSLRTRVSFEVAMRHLGGDALYLSPNEIGLGERESVADVARVLSGMVEGIMA
                     RVFGHELVVGLAEWATVPVINGLSDASHPAQIMADFLTIREKFGNFDGLNLAYIGDGN
                     NVSVSLMQMSAILGTNFIIASPEGYDMEDRFVDESKAIAAKTGAKLSFFRDPHEAVKG
                     ANVIYTDTWISMGQEEESAPRLKVFPPYQVNEQLVAEADPDVMVLHCLPAYRGIEITD
                     AVCDGPNSWLFPQAHNRLHAQKAILYKLMKD"
     gene            complement(1181..2026)
                     /locus_tag="PK791_05790"
     CDS             complement(1181..2026)
                     /locus_tag="PK791_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92857.1"
                     /translation="MPSKLVQFIKQNKTRIDLAKSGSSLILGIAMLVLLIVLAVTLAD
                     GTRELIHIASTGIDTLQETLSSVNDGLNNVSSSFDTLKDSLQTVKDYVYGIEPILENM
                     TDLVGSDLVKIAQDGQNSLKAAAQGSKLVDDALTLLSRLPLLNFDYAPEKALNESLLE
                     LSETFGSIPESLSGLEKEISNSAANISTFDTNFVDFNTNIDDLKANVDNTGSSLEGFI
                     DQLQSYQQKLPKIQRTIITWIAIITCALCLLLLIWMVNQFFTFIMALEAIKSDKIAMR
                     KRPEA"
     gene            complement(2016..>2336)
                     /gene="ybeY"
                     /locus_tag="PK791_05795"
     CDS             complement(2016..>2336)
                     /gene="ybeY"
                     /locus_tag="PK791_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOH92858.1"
                     /translation="IRSLNRDYRGLDQATDVLSFPIGFDDPETGSFYLGDIIISLTMA
                     NKQAAQSNTTLQAELEMLMVHGILHLCGYDHDTAENYAAMSSLQDEILQSVQNPLTGS
                     IHAQ"
BASE COUNT          582 a          495 c          605 g          654 t
ORIGIN      
        1 ttttgtgtgc catgcgagaa gttgagacgg gcaacgtcca aaccggcttc aatcattttt
       61 ccaagcgtgg tttcatctga gctggcaggg cctaaagttg acacaatttt cgcgaaacgt
      121 tccatggtta tctccatata ctaaaagcga cccggtagtg tatgttattt ataccgggtc
      181 gcatggttgt ttggggttta gtccttcatc aatttgtaaa ggatagcttt ttgggcatgc
      241 aagcggttgt gggcttgcgg gaagagccag ctgttaggtc catcgcagac tgcgtcggtg
      301 atttcaatac cacgataagc aggcagacag tgtaatacca tgacatcggg gtctgcttct
      361 gcaactaatt gttcgttcac ctggtaaggt gggaaaactt taaggcgtgg agcggattct
      421 tcttcctggc ccatgctaat ccaggtgtcg gtataaatga cattagcacc tttaaccgct
      481 tcgtgtgggt cgcggaagaa agataacttg gcgccggtct tcgcggcgat ggctttgctt
      541 tcatccacaa agcggtcttc catatcgtag ccttcggggc tggcgatgat gaagttcgtg
      601 ccaagaattg ctgacatttg catgagtgat acggaaacgt tgttgccatc accgatatag
      661 gccaggttga gtccgtcaaa attaccgaat ttctctcgga ttgtcaggaa gtcagccata
      721 atttgagcgg ggtggctggc atcgctaagg ccgttgataa cagggacggt tgcccattct
      781 gccaggccaa caacaagttc gtgtccaaaa acacgtgcca taatgccttc aaccatgcca
      841 gacaatacac gggcaacatc agctacggat tcgcgttcac ccagaccgat ctcgttggga
      901 gaaaggtaga gcgcgtcgcc accgaggtgt cgcatggcaa cttcaaagct cacgcgggta
      961 cgtaacgaag gtttttggaa aatcatcgcc aggtttttgc ctttgagaac aggttcgttg
     1021 ccaccggcaa aatattcttt tttgagttcg gtagcgaggt ccagaaaacc taataattct
     1081 tctttgctga agtccgtgat gtcaagaaag tcttttttca tttttactcc ttataaaagt
     1141 gttttgaact gccataaaaa acgccggaac atggctttgt ttatgcttcc ggtcgctttc
     1201 gcatggctat tttatcagac tttatggctt cgagtgccat aatgaaagta aaaaactgat
     1261 ttaccatcca aattaacagc agaaggcaca aagcacaggt gataatagct atccaggtga
     1321 tgattgtgcg ttggattttg gggagctttt gctgatagct ttgcagttgg tcaataaagc
     1381 cttccagtga tgatccggta ttatcgacgt tggcttttaa atcgtctatg ttggtattaa
     1441 agtcaacgaa attcgtgtca aaagtgctaa tattcgcggc tgaattgctt atctcttttt
     1501 ccagcccgga aagactttcg ggaatactgc cgaaagtttc agaaagctca agaagacttt
     1561 cgttaagggc tttttcgggc gcgtagtcga aattgagcaa gggcaagcgc gagaggagcg
     1621 ttagagcgtc atcaacaagt tttgaaccct gggcggcggc ttttaaagaa ttttgtccat
     1681 cctgggcgat ttttaccaaa tcgctgccta ccaggtctgt catgttttca agaatgggtt
     1741 cgataccata aacatagtct tttacggttt gtaaggagtc tttgagcgtg tcaaagcttg
     1801 aggaaacgtt gttaagacca tcgttgacgg aagacaaggt ttcctgtaag gtatctattc
     1861 cagtgctggc gatgtggata agttcgcggg tgccatcagc cagcgtgacc gctaagacga
     1921 tgagcagcac aagcatagcg atgcccaaaa taagcgaaga accacttttt gcgaggtcga
     1981 tgcgtgtttt gttctgtttt ataaactgaa cgagtttact gggcatgaat actcccggtt
     2041 agcgggtttt gaacgctttg caaaatctca tcttgcaggc tgctcattgc agcgtagttt
     2101 tcagcggtgt cgtggtcata gccacagaga tgcaaaatgc catgcaccat gagcatttcc
     2161 agttctgcct ggagcgttgt gttggattgc gcggcttgtt tgttcgccat cgttagtgag
     2221 atgatgatat cgccaagata aaaactgcca gtttcgggat catcaaagcc gatgggaaac
     2281 gagagcacat cggtggcctg gtcgagaccg cgatagtcac ggtttaggct acggat
//
LOCUS       DAOSFC010000225         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_45653_length_2312_cov_3.264953, whole genome shotgun sequence.
ACCESSION   DAOSFC010000225 DAOSFC010000000
VERSION     DAOSFC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45653_length_2312_cov_3.264953"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PK791_05800"
     CDS             complement(<1..450)
                     /locus_tag="PK791_05800"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOH92859.1"
                     /translation="MDNNSIVPVENIDIYKIIYTIRDKQVMVDSHLAALYKVETRVFN
                     QAVKRNIERFPEHFRFQLTDEEYRNLKSQTVISSSDSQHGGRRYNPYVFTEQGIAMLS
                     AVLRSDVAIEVSIKIMDAFVEMRKFLSSNKQLFARLDKVELKQIETDK"
     gene            complement(702..>2312)
                     /locus_tag="PK791_05805"
     CDS             complement(702..>2312)
                     /locus_tag="PK791_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92860.1"
                     /translation="PDDRILAILVGAVIFIEILGYVFFHIQQRLMARVNADVYRSLAT
                     DAFAASMEQDLAFHDKLSSGRIVSRITGDTEDFITLIRLTMNVLSSVMQSIIVAIILL
                     RTEWRLALMLFIFVPIVILIVSSYRNVARRVTTRGMRAMANVNATVKETISGISVAKN
                     FRQEESIYADFQESNKTSYNVNVKRGFVLSIVFPTMRTLSGISIALLVYFGALSVSQG
                     LITAGAWYLVLLSSERFLMPILSMTSYWAQVQTGFAAAERIFALIDAEHTVIQTSDKP
                     ARALHGDIVFENVSFSYGVGAEVLNKFYLHIKPGENLAIVGHTGAGKSSIARLISRFY
                     EFQEGRILIDGTDIREFNIDSLRQQMGVVTQVPFLFEGTVEENIRFAQPDITREEILA
                     LAHEIGDGEWLETLTNGLDTNVGERGAKISMGQRQLVALMRVLARKPAIFILDEATAS
                     IDPFTETQIQHALNLILSQSTSVLIAHRLSTVKSADRIIVLDHGGIIEEGTHDALLEK
                     GGTYAELYNTYFRHQSLEYVEEAGKFLK"
BASE COUNT          607 a          502 c          579 g          624 t
ORIGIN      
        1 tttatctgtt tctatctgct ttagttcaac tttgtcaagt ctagcaaaaa gttgcttgtt
       61 gctactaaga aattttcgca tttctacaaa agcgtccatt attttaatgc tgacttctat
      121 cgcgacatca gatctcaata ctgcggacaa catggcaatt ccctgttccg taaaaacata
      181 ggggttatag cgtctgccac catgttgact atcagaactt gagatcacag tttgtgattt
      241 caagtttcta tattcttcat cagtcaactg aaagcgaaaa tgctctggga atctttcaat
      301 attccttttt acagcttgat taaaaactct ggtttcaact ttgtatagag ctgcaagatg
      361 gctatctacc atgacttgtt tatctctgat tgtgtagatg attttataaa tgtcaatgtt
      421 ttcaacaggc acaatgctat tgttatccat gatagtttgt ctcattattg gattgaatca
      481 ttattaggtc tccatcatcc gcaactttta tttattatga aacgcactct atttaagtac
      541 acagattttc ttaataataa tttatggttt cattatatca ttgtcaaaat catcaaagac
      601 tagttagctg tagctggagt ctagtcataa aaagtgcaaa agtttgaaaa tgggttttcg
      661 aaacctgcat atctaaactc tcaggcgaac aagtcgcaaa actacttaag aaacttgccg
      721 gcttcttcga catactccag cgactggtgg cggaagtagg tgttgtagag ttcggcgtag
      781 gtgccacctt tttcaagaag cgcgtcgtgg gtgccttctt caatgatgcc accatggtcg
      841 agcacaatga tgcggtcagc agattttact gtggacaaac ggtgagcgat gagtacactg
      901 gtggactgac tcaaaatgag gttcagcgcg tgttgaattt gtgtttcggt gaaggggtca
      961 atactcgctg tggcttcatc cagaataaag atggcaggtt tacgtgccag aacgcgcatc
     1021 aaagcgacga gttggcgttg ccccattgag attttcgcgc cacgttcgcc aacgttggtg
     1081 tcaagtccgt tggtgagcgt ttcgagccat tcgccatcgc cgatttcatg tgccagggcg
     1141 agaatttctt cgcgggtgat atcaggctgg gcgaagcgga tgttttcttc cactgtgcct
     1201 tcaaacagga agggtacctg ggtgaccaca cccatttgct gacggagact gtcgatgttg
     1261 aactcgcgga tgtcggtgcc atcaatcagg attctgcctt cctggaactc gtaaaagcgt
     1321 gaaataaggc gcgcgatgga cgatttgccc gcgccagtgt gaccaacgat cgcaaggttt
     1381 tcgccaggtt tgatgtgaag atagaattta ttcaagactt cagccccaac accgtaactg
     1441 aagctgacat tctcgaatac gatgtcgcca tgcagagctc gtgcaggttt gtcgctggtt
     1501 tgtatcaccg tgtgttcggc atcaatgagc gcgaaaatgc gttccgccgc cgcaaagccg
     1561 gtttgcacct gcgcccagta ggatgtcatg cttaaaatgg gcatgagaaa acgttcgctg
     1621 gagagcaaaa ccaggtacca ggcacccgcg gtgatgagcc cctggctaac cgagagcgcg
     1681 ccgaagtaga ccaaaagcgc gatactgatg ccacttagcg tgcgcatggt ggggaaaacg
     1741 atggacaaga caaaaccacg cttgacgttg acgttgtatg aggtcttgtt agattcttga
     1801 aagtcagcgt aaatactttc ttcctgccgg aagtttttgg caacggaaat gccactgatg
     1861 gtttctttga cggtggcgtt gacgttagcc atagcgcgca taccgcgggt ggttacgcgt
     1921 cgggcaacgt tgcggtagga agacacgatg agaatgacaa tgggcacaaa gataaacagc
     1981 atgagtgcca ggcgccattc ggtgcgcaac aaaatgatgg ccacgatgat ggattgcatc
     2041 actgagctta agacattcat ggtcaggcgt atgagcgtga taaagtcttc tgtgtcaccg
     2101 gtgatgcgcg aaacgatgcg accggaactg agtttatcgt gaaaggcgag atcctgctcc
     2161 attgaggcgg caaacgcgtc agttgcgagc gagcggtaaa catcggcgtt gacccgcgcc
     2221 atgagccgtt gctggatatg aaaaaaaaca tagcctaaga tttcgataaa gataaccgcg
     2281 ccaaccagaa tagcaagtat acggtcatcg gg
//
LOCUS       DAOSFC010000226         2294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_46177_length_2294_cov_3.914694, whole genome shotgun sequence.
ACCESSION   DAOSFC010000226 DAOSFC010000000
VERSION     DAOSFC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46177_length_2294_cov_3.914694"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PK791_05810"
     CDS             complement(<1..1065)
                     /locus_tag="PK791_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH92861.1"
                     /translation="MTDKNDLEDNARIEETQELLENIDDPVKLYLREITSTPLLSNEE
                     EFRLALAVQADELAKNCSNELTGMNLQTILADIQHSWESVINDAHRLKHNPPDVMAIL
                     KEVRAFQDELLPDEPSYTRAFLDLPRWGNDRVWEVFATNILRFFQAVFLLGSSNLDLL
                     IQHLQKHDETILDALGKIDFNADLIETRARVSERAKQASDILVEYNLRLVVSIAKHYS
                     NPGVSLLDLIQEGNIGLMRAISKFDPAKGYRFSTYAAWWIRQSVSRYIAEHARTIRLP
                     IHVAEAIGRLNKIQRDLVQRFGREPSFAEVAAHSTYLSDEDQAAIEAIGFDREKAPPD
                     LIYRWDEATRKVEDMLKKRET"
     gene            complement(1090..>2294)
                     /locus_tag="PK791_05815"
     CDS             complement(1090..>2294)
                     /locus_tag="PK791_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOH92862.1"
                     /translation="GMDLARREMRQLNQAVIVEGYMDVIALHQAGYKNVVASMGTALT
                     EEQFGLLKRTTRNIVLALDADQAGQNATLRGLETARRTMGEELDMTFDSRGLLRFERH
                     LKADIRVTTLPEGLDPDEIVNEDPEQWRAIVANAKPIVVHVMQTLAQNQDLDDAKVKR
                     EIAAQVMPLIEDIADAVEREGYRQQLANFLRIDERALRTSPDPGGSQTRRRRHAEPET
                     EQTLPSPALANLSKRNRLLETHILSFLIRAPETVYKLNRQLGILNLSLLSQDDFQESD
                     CKLAFGIVQASLEQDAEPAIDYLEQHLPDIFDPIEPQSPDLVILEPSKEKQLLDQLKS
                     VLRLRKNIIEDRQQEMQFIQSDPEMQELSKEEASSLYIELLNKRRTIDHALASPFLPE
                     DKINKAKN"
BASE COUNT          476 a          518 c          659 g          641 t
ORIGIN      
        1 cgtttcgcgc tttttgagca tatcttcaac tttacgcgtg gcttcgtccc agcggtaaat
       61 caaatctggc ggggcttttt cgcggtcaaa gccgatggct tcaatggctg cctggtcttc
      121 atcacttagg taggtggaat gcgcggcaac ttcggcaaaa cttggttcac ggccaaagcg
      181 ttgtaccaaa tcacgctgga ttttgttcag gcgtccgatg gcttcagcca catgaatggg
      241 caggcgaatg gtgcgggcat gttcggcgat ataacgcgag acggactggc gaatccacca
      301 ggcagcatag gtgctaaaac gatagccttt ggcggggtca aacttggata tcgcgcgcat
      361 gagacctata ttgccttcct gaatgagatc caaaagggaa acaccagggt tggagtagtg
      421 cttggcaata ctcaccacaa gtcgcaaatt gtattccacc aagatatcgc tggcttgttt
      481 ggctctttca ctgactctcg cacgggtttc gatgagatcg gcgttgaagt caattttgcc
      541 tagcgcgtca agaatggttt catcatgctt ttgaagatgt tgaataagta aatccaggtt
      601 tgaagaccct agcaagaaaa cggcttgaaa aaatcgcaag atgttggtag cgaagacttc
      661 ccaaacgcgg tcatttcccc agcggggaag gtctaaaaag gcgcgtgtgt aggaaggttc
      721 atcgggcagg agttcatcct gaaaggcgcg gacttctttt agaattgcca tgacatcagg
      781 cgggttgtgt ttcaggcgat gggcgtcgtt gatgacgctt tcccagctgt gttggatatc
      841 agccagaatg gtttgcagat tcatgccggt cagttcgttt gaacagtttt ttgccagttc
      901 atcagcttgt acggcaagcg ccaggcgaaa ttcttcttcg ttacttagca agggggtgct
      961 ggttatctct cgcaaataga gttttacagg gtcatcgatg ttttccaaaa gttcctgggt
     1021 ttcttctatg cgggcattat cttcaaggtc gtttttatcg gtcattattg tctttctgtg
     1081 ggttaggcac taatttttgg ctttgtttat tttatcttca ggcaggaatg gtgatgccag
     1141 cgcgtggtca atggtacgcc gtttgttgag tagttcaata taaaggctgc tggcttcttc
     1201 tttggaaagc tcttgcatct ccggatcgga ctggatgaac tgcatttcct gctggcggtc
     1261 ttcaatgatg tttttgcgaa gtctcaaaac gcttttcagc tggtcgagaa gctgtttttc
     1321 tttgctgggc tccaggataa ccagatctgg actttgtggt tctatggggt caaagatatc
     1381 aggcaagtgt tgctcaaggt agtctatggc gggttcggcg tcttgttcaa ggctggcttg
     1441 cacaatgcca aatgccagtt tgcagtctga ttcctgaaag tcatcctggg atagcaggga
     1501 caagttgagt atgcctagct ggcggtttag tttgtaaacc gtttcgggcg ctctgatgag
     1561 aaaactgagg atgtgcgttt caagcaggcg attgcgtttg ctgagatttg ccagggcagg
     1621 gctgggcaag gtttgttctg tttcaggctc ggcatgacgg cgtcttcgcg tttggcttcc
     1681 accggggtcg gggctggtgc gcagggctct ttcgtcaatg cgcaggaagt tggccagttg
     1741 ctggcgatag ccttcgcgtt ctaccgcgtc ggcgatgtct tcaatgagag gcatgacctg
     1801 agcggcaatt tcgcgtttga ctttggcgtc atccagatcc tggttttgcg ccagggtttg
     1861 catcacgtgc acgacaatcg gtttggcgtt ggcaacgata gcgcgccatt gttcgggatc
     1921 ttcgttcacg atttcgtcgg ggtcaagccc ttcgggcagg gtagttacgc ggatatcagc
     1981 tttgagatga cgttcaaagc gcaacaagcc ccgcgagtcg aaagtcatat cgagttcttc
     2041 acccatcgtg cgacgggcgg tttcaagccc gcgcagggta gcgttttggc cggcctggtc
     2101 ggcgtcgaga gccagaacga tgttgcgcgt cgtgcgtttt aagagtccaa actgctcttc
     2161 ggtaagcgcg gtacccatcg aggcgaccac gtttttatag ccggcctggt gaagcgcgat
     2221 cacgtccata tagccttcaa cgataacggc ctggtttaac tggcgcattt cacgacgcgc
     2281 aaggtccatg ccgt
//
LOCUS       DAOSFC010000227         2267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_46932_length_2267_cov_5.638336, whole genome shotgun sequence.
ACCESSION   DAOSFC010000227 DAOSFC010000000
VERSION     DAOSFC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46932_length_2267_cov_5.638336"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK791_05820"
     CDS             <1..185
                     /locus_tag="PK791_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOH92863.1"
                     /translation="LKIAGQSIHGIIPSYGHRSPGDLVAVTDSEGFVEVAVVNGSAAR
                     TFGIQLDDPVEVFFDE"
     gene            178..1803
                     /locus_tag="PK791_05825"
     CDS             178..1803
                     /locus_tag="PK791_05825"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92864.1"
                     /translation="MSEPKPLPLVVKDLSFRYHTRDSCAIRNINLSLEPGQIMLLAGS
                     SGCGKTTLMRCINGLIPHSYNGEMTGEILVHGQSIEGMKLSQISRHIGTLLQDPERQI
                     LGTYVLNEVCFGLESMALPRKEMLPIAHKALERLRILYLKDKETFFTSGGEKQKVALA
                     GVLAMEPDILLLDEPLASLDPASAHEALLAFRELADEGLSIMIVEHRVDDVLAIKPEK
                     VLYMEDGEINYYGDVAGLMETVDYSRIKLPAEVIARRAKGKVHPVFKPTVPELDKDSE
                     PLLSFQDVRFQYKEDTPEVLHGINLDVRSGEVIAILGHNGSGKTTMVKHALGLLKPTS
                     GKVLLEGVDSKKSTIATSAKTIGYVFQSPSQMLFASTVREELAYGPENLGFSKEQVAE
                     NVDWAIDTVNLEEELSTPPLALSFGQQKRVSIASVLSMRSRILMMDEPTAGQDYWNYK
                     SFMDTILQTPGFDSIIFITHDLDLALIYANRVIVLYGGEIVADGHPQDILLDEEKIKK
                     WRLLPTSLLKLNQELWPKTGQYMRAEALSQFIT"
     gene            1822..>2267
                     /locus_tag="PK791_05830"
     CDS             1822..>2267
                     /locus_tag="PK791_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOH92865.1"
                     /translation="MAKQTEKKSIWAFGTKQVVYAAIGAALYAVLTWAFNVWQIPGAG
                     NVGIRPAIVIPMFFGIAFGPIVGFITGFVGNILADMLSGYGFWIWWDLGNGIIGLIPG
                     LFSALIAKYDEVKSILWAELSVILGSAAGMGLASLSELWVSGADMNT"
BASE COUNT          572 a          570 c          538 g          587 t
ORIGIN      
        1 cgctcaaaat cgccgggcaa agcattcatg gaatcatccc atcctacgga caccgctcgc
       61 ctggagacct ggtggcggta accgacagcg aagggttcgt tgaagttgcc gtcgtcaacg
      121 gcagtgccgc aaggaccttt ggcattcagc ttgatgatcc agtggaggtc ttctttgatg
      181 agtgaaccga aacccttgcc actggttgtt aaagacctca gctttcggta tcacacgcgc
      241 gactcctgcg ctattcgcaa cattaattta tcgcttgaac ccggtcaaat catgcttttg
      301 gccggttctt caggttgtgg caaaaccaca ctgatgcgct gcatcaacgg acttattccg
      361 cattcctaca atggtgaaat gaccggcgag attctcgtgc atggtcagtc cattgaaggc
      421 atgaagcttt cacaaatttc aagacacatc ggcaccttgc tacaggaccc tgaacgacaa
      481 attttgggca cttatgtctt aaatgaagtc tgtttcgggc ttgaatcaat ggcattgcca
      541 cgcaaagaga tgcttcccat cgcgcacaaa gcgcttgaaa gactacgcat cctgtatctt
      601 aaagacaaag aaaccttctt tacctctggt ggggaaaaac aaaaagttgc cttggcaggc
      661 gttcttgcca tggaaccaga catccttttg cttgacgaac cgctcgccag ccttgacccc
      721 gcatccgccc acgaagcctt gttagccttt cgtgagctgg cagatgaagg tctttcgatc
      781 atgattgttg agcaccgcgt tgatgacgtc ttggctatca aacctgaaaa agttctctac
      841 atggaagacg gtgagattaa ctactacggc gatgttgccg ggctgatgga aacagtggat
      901 tacagccgca ttaaattacc cgctgaagtg attgccagac gcgccaaagg caaggtgcat
      961 cctgttttca aacccaccgt ccccgaactt gacaaggatt ccgaaccctt gctttcgttc
     1021 caggatgttc gttttcaata caaggaagac actcccgaag tgctgcatgg tataaatttg
     1081 gatgttcgtt ccggcgaagt cattgctatt cttgggcata acggctcggg aaagaccacc
     1141 atggtgaagc acgccctggg attactcaaa cctaccagcg gtaaagtttt actggaaggt
     1201 gtcgattcca aaaaatcgac catcgctact tcggccaaaa cgatcggtta tgtcttccaa
     1261 agccccagcc aaatgctttt tgcctccaca gtccgcgaag agcttgccta cgggcccgaa
     1321 aatctcggct tctcaaaaga gcaggttgca gaaaacgttg actgggctat cgataccgta
     1381 aaccttgaag aagaactgtc caccccacca ttggcacttt cctttggaca gcaaaagcgg
     1441 gtctccatcg cttcggtgct ttcaatgcgc tcgcgaatct taatgatgga cgagcctacc
     1501 gccgggcaag actactggaa ctataaatcc tttatggaca ccatcctgca aacacccggt
     1561 tttgactcga ttattttcat cacccacgac cttgatctcg ctttaattta tgccaatcgt
     1621 gtgattgtgc tttatggtgg cgaaatcgtt gctgatggac atccacaaga tatattatta
     1681 gatgaagaaa aaatcaagaa gtggcgctta ttgccaactt ctctgctaaa attgaatcag
     1741 gaattatggc caaagactgg ccaatatatg cgcgctgaag cgcttagcca atttattaca
     1801 taatacaaag gagaaatatt tatggcaaaa caaactgaga aaaagagtat ttgggcattt
     1861 gggaccaaac aggtcgtcta tgccgccatc ggagccgcgc tttacgcggt gttaacctgg
     1921 gcttttaacg tttggcagat ccccggagct ggcaacgttg gtatccgccc agcaattgtt
     1981 attcctatgt tcttcggtat cgccttcggt ccaatcgttg gctttatcac cggctttgtg
     2041 ggcaacatcc tcgccgacat gctcagtggt tatggctttt ggatttggtg ggatttgggc
     2101 aacggtatca tcggcttaat ccctggcctg ttctcagcct taatcgccaa atacgacgaa
     2161 gtcaaatcaa ttctttgggc tgagctttcg gtcattttgg gtagcgcggc aggcatgggt
     2221 ctggcaagcc tctcagaact ttgggtgtct ggcgcggaca tgaatac
//
LOCUS       DAOSFC010000228         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_47031_length_2264_cov_6.507017, whole genome shotgun sequence.
ACCESSION   DAOSFC010000228 DAOSFC010000000
VERSION     DAOSFC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47031_length_2264_cov_6.507017"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="PK791_05835"
     CDS             complement(<1..1344)
                     /locus_tag="PK791_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH92866.1"
                     /translation="MKNHKTPKILLKLIMVLIAFAMTLGAFSHSAKAQYQPGEWLPFG
                     TVTQNALPEVNLLSANADEIMVYAEMPGITVSETELFGQSFLSFEMEGYHVTQDVGAP
                     ALPVLNQLIEVPLGAEITLELIDSEIQTIKLAEHGLNPMIAPVQPGQPKCEGYVPGGE
                     PLAEFYENGFYPESPIAIVDEFIMRGHRVVNVQMRPVRYNGTTGELETRASMSFKLLL
                     SGSDMDLTNQEADRLNAEPFNNILAPTVLNFNQGRPVAVPNTAERILIITADAFESTL
                     ADLVTLKQSQGFDVSVVNLTTVGGNTTTAIKNYIKAQYQGANPPVYVYLIGDTKNSAH
                     STWTLTNYSFRSSGGSGASTDLHYFTMDSDTEFVPDIFWGRFPVRELSHLQNMIANLE
                     WFNETSGDEPWVKKAEFLASDDSWNYDVAEGTHNYCIDNYTLPRGYTGIFPQNPQP"
     gene            complement(1481..2110)
                     /locus_tag="PK791_05840"
     CDS             complement(1481..2110)
                     /locus_tag="PK791_05840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92867.1"
                     /translation="MDAAFFDRWSLEYDDDVIACDEANLYPFAGYRRTLESLLDAIKA
                     KPGAKVLDLGFGTAKLTQKLYDTGYEISGLDFSPEMCRIAQEKMPKAQLICFDFAQGL
                     PDAIKDARFDIIVSSYAFHHVPDSLKPSRIVEWYDALNPGGVILIGDVAFLNEQSLEL
                     SRDDAGDEWDEDEDYMVFSSLQPEIAHLSPKYEQISHCAGFLTIYKTYC"
BASE COUNT          623 a          537 c          497 g          607 t
ORIGIN      
        1 gggctgggga ttttgcggga aaatacccgt atacccacga ggcaaggtgt agttgtcgat
       61 acagtaatta tgggtacctt cagcaacatc ataattccat gagtcatccg aagccaggaa
      121 ttcggctttc ttcacccaag gttcatcacc tgaagtttca ttgaaccact ccaggttggc
      181 aatcatattt tgcaagtgcg aaagttctcg cacagggaaa cgtccccaaa aaatatcggg
      241 cacaaattct gtatcgctgt ccatcgtgaa gtaatgcaaa tctgtagacg caccagaccc
      301 accggaagat ctgaaagaat agtttgttag tgtccaggtt gaatgggcgc tattcttggt
      361 atcaccgatc agataaacat aaacaggtgg attagcacct tgatattggg ctttaatata
      421 gtttttaatg gctgttgtgg tatttccacc aacggttgtc aggtttacga cagagacatc
      481 aaatccctgt gactgtttaa gcgtcaccaa atcagccaga gtactttcaa acgcgtccgc
      541 agtaattatt aatatgcgtt cggcagtatt gggaacagca accggacgtc cctggttgaa
      601 gttcagtact gttggtgcaa gaatgttatt gaatggctca gcattaaggc ggtcagcttc
      661 ctgattggtc aggtccatgt cactgccact aagcaaaagc ttaaaggaca tgctcgcgcg
      721 ggtttccagt tctcccgtag tgccattgta gcgcactggg cgcatttgca cgttgaccac
      781 gcggtgacca cgcataatga attcatccac aatggcgatc gggctttcag gatagaagcc
      841 attttcgtaa aactcggcca gtggttcgcc accgggcaca tagccttcac atttaggctg
      901 gccaggttgc accggcgcaa tcatgggatt aagcccatgt tcggcaagtt ttatggtttg
      961 aatttcacta tcgatgagtt ccagggtgat ttccgcgcca aggggcactt cgattagctg
     1021 gttgagcaca ggcaaagccg gcgcgccaac atcctgggtc acatggtagc cttccatctc
     1081 aaatgagagg aagctttgac caaacagttc ggtttcgctt acggtaatgc caggcatttc
     1141 agcataaacc ataatttcat cggcattcgc actgagcaaa tttacttcag gcaatgcgtt
     1201 ttgagttacc gtgccaaagg gcaaccattc acctggttga tactgggctt tcgcgctatg
     1261 cgaaaaagcg cccaaagtca tcgcgaacgc gatcaggacc atgattagct ttagcaggat
     1321 ttttggggtt ttgtgatttt tcatcagctc tcctattaac aaattagttg aacggctgtt
     1381 atgtgcctta agcacacaaa cagaaatttt aacccataat cacaaattca tgggaattaa
     1441 aaacaaaatt aaaatcaatc tgaactcaat acgaattaaa ttagcaatat gtcttgtata
     1501 tagtcaaaaa accagcacaa tgcgagatct gctcatactt tggcgagaga tgcgctattt
     1561 caggctgtaa agatgagaaa accatgtaat cttcgtcttc atcccattcg tcgccagcgt
     1621 cgtctctgct tagttccagg gattgctcat ttaagaaagc gacatcacca atcagtatca
     1681 cgcctcccgg atttaaggca tcataccact ctacaatccg cgacggctta agagaatctg
     1741 gcacatgatg aaaagcgtaa gaactcacga tgatgtcaaa gcgcgcgtcc ttgattgcgt
     1801 ccggtaatcc ttgcgcaaaa tcaaagcaaa tcaactgtgc tttaggcatc ttctcctgcg
     1861 cgatacgaca catttctggc gaaaaatcca aaccactgat ttcatagccg gtatcataca
     1921 acttttgggt cagtttcgcc gtcccaaaac ccaaatcaag aacttttgcc ccaggtttgg
     1981 ctttaatagc atccagcaag ctttccagcg tgcgtctata gcccgcaaaa ggatacagat
     2041 ttgcctcgtc gcaagcaatc acatcgtcat catattccaa agaccatcgg tcaaaaaaag
     2101 cagcgtccat cttcacacac ccgttgagat ccttggtctc agcaagtcat ttcgggtcaa
     2161 tgataatcag tttcgttgac aattcaaggg cttgcatcta aacttgattt atgcttgccc
     2221 acgtttattc ttgcgctgtt atcggtctgg atggtgtcac aatt
//
LOCUS       DAOSFC010000229         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_47143_length_2261_cov_3.211695, whole genome shotgun sequence.
ACCESSION   DAOSFC010000229 DAOSFC010000000
VERSION     DAOSFC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47143_length_2261_cov_3.211695"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PK791_05845"
     CDS             complement(<1..564)
                     /locus_tag="PK791_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH92868.1"
                     /translation="MNFFQAVKEAFSSLAANKMRSFLTVLGIVIGVGAVIAVLSISRG
                     AEASIIGQIESIGTNVIYVMRGNRAEDVKNPRHLTLADVEELNNRLRAPHVRWASPMM
                     SEFASFSLGSESHEAGVVGSVPVYQYTMDVQLTEGEFFTEDDVDSRRAVVVLGPELAK
                     RLTGRSSEVVGMSVRINNYPFRVIGVSA"
     gene            complement(569..1267)
                     /locus_tag="PK791_05850"
     CDS             complement(569..1267)
                     /locus_tag="PK791_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92869.1"
                     /translation="MADKPHVEPVIRVRGMTKDFQMGEDIVVRALRGIDLDIYPGELT
                     TIMGPSGSGKSTLMNMIGCLDSPTSGDYYLDGVLVSDLEDDELAEVRNKKIGFVFQKY
                     NLLPRISAIENVALPLRYSDDTSNMRQRAEEALIAVGLEDRMLHKPNELSGGQQQRVA
                     VARALVNKPAIILADEPTGNLDSKSGQEVMDLLLKLNHEMKTTIVIVTHDADVAAQTR
                     RVIRLFDGLIVSED"
     gene            complement(1257..>2261)
                     /locus_tag="PK791_05855"
     CDS             complement(1257..>2261)
                     /locus_tag="PK791_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH92870.1"
                     /translation="LRNLESDTDLQRAQLQSDITTVEKNIANLQDQRDALTLRTCSES
                     RLTTLQKQLDDALADYENWPTQVRWMAVENARQNLAFCDPQSIADEINTLDRQIALQE
                     EILTGHQANLEKIKDGPDPEVKERLQAQLDLANKQLSQQEILAPFAGTITELSMQVGD
                     GVSPGSRAVQMADLSELYLDVPVSEVDIPYIQVGQSAELIFDAFFEETYQGIVSEIAS
                     VPSMAGGVVNYNVTILMQSGMDKVKPGMTVGVNILTETRENTYVVPAESVFTRDGKTY
                     VYVRRDGRPEMVEVKIGAYSSQSIEILEADIQDGEAIYISPPVSLIESFMRMGGGHGR
                     "
BASE COUNT          526 a          647 c          505 g          583 t
ORIGIN      
        1 cgcgctcacg ccaataaccc tgaaaggata attattgatg cgcacactca tccccacaac
       61 ttcactgctt ctgcctgtca ggcgtttggc taattcgggg cccaatacaa ccaccgcgcg
      121 acgcgagtca acatcatcct ccgtgaaaaa ttcgccttcc gtcagttgca cgtccatggt
      181 gtattgatac accggcacgc tgcccacaac accagcttcg tggctctcac tacccaaact
      241 gaagctggcg aactcgctca tcatcggcga tgcccaacgc acatggggcg cgcgcaaacg
      301 gttgttcaat tcttcaacat ccgccaaagt gagatgcctg gggtttttga cgtcctccgc
      361 gcgattgccc cgcataacat agataacatt cgtgccaatc gactcaattt gcccgataat
      421 actcgcctca gccccacggc tgattgacaa aaccgcgatg accgcgccca caccgataac
      481 gatgcccaaa acggtgagaa aggagcgcat tttgttggct gctaaactgc taaaagcttc
      541 ttttactgcc tgaaaaaagt tcatcgcgct agtcctcact cacaatcaag ccatcaaaca
      601 ggcgtatcac gcgtcgtgtt tgtgcggcga catcagcgtc atgggtgacg atgacaatcg
      661 ttgttttcat ttcgtggttg agtttcaaaa gcaaatccat cacttcctgt ccggattttg
      721 agtcaaggtt tccagtcggt tcgtctgcca aaataattgc cggcttgtta accaaagccc
      781 gtgccaccgc cacgcgttgt tgttgcccgc ccgaaagctc gttgggtttg tgcaacatgc
      841 ggtcttccag tcccaccgca atcagggctt cttctgcgcg ttgccgcata ttgctggtat
      901 catcgctgta ccgcaaagga agtgccacat tctcgatggc agagatgcgt ggcaaaagat
      961 tgtacttctg aaaaacaaaa ccgatttttt tgttgcgcac ttcggccagc tcatcatctt
     1021 caaggtctga aaccagcaca ccatccaggt aatagtcacc gctggtcggt gagtcaagac
     1081 aaccgatcat attcatcaag gtcgattttc ccgaaccgct cggccccata atcgtggtca
     1141 gttcaccggg ataaatatca aggtcgatgc cccgcaaggc gcgcacgacg atatcttcgc
     1201 ccatctgaaa gtctttggtc atgccacgta cccggataac cggttccacg tgcggtttat
     1261 ctgccatgcc caccacccat gcgcataaag ctttcgatta aactaaccgg cggcgagatg
     1321 taaatcgctt cgccatcttg gatgtccgct tccagaattt caatcgactg gcttgaataa
     1381 gcgccgattt tcacttcgac catctccggt cggccatcgc gacgcacata gacataagtt
     1441 ttgccatcgc gcgtaaagac cgattcagcc ggcacaacat aggtgttctc gcgggtctcg
     1501 gtgaggatgt tcacgccaac cgtcattccg ggtttgactt tatccatgcc gctttgcatt
     1561 aaaatcgtca cgttgtaatt caccacaccg cccgccatgc tgggcacgct ggcaatttcg
     1621 ctcacaatgc cctggtaagt ttcttcaaaa aaagcgtcaa aaatgagctc cgcggactgt
     1681 ccaacttgaa tataaggaat atccacttcc gaaacaggca cgtccagata gagctcgctc
     1741 aaatccgcca tttgcacagc cctgcttccc gggctaacac catctcccac ctgcatgctc
     1801 agttcggtta tcgtgccagc gaatggcgcg agaatttcct gttggctcaa ttgcttattc
     1861 gccaaatcga gctgagcctg caaacgctct ttcacttcag gatcaggccc gtctttaatc
     1921 ttttcgagat ttgcctgatg tcctgttagg atttcttcct gtaaagcaat ttgcctgtca
     1981 agcgtgttta tctcgtcagc aatcgattgc ggatcgcaaa aagcgaggtt ttgacgagcg
     2041 ttctccaccg ccatccatct gacttgcgtt ggccagtttt cgtaatccgc taaagcgtca
     2101 tcaagctgtt tttgtaaggt cgttaatcgt gattccgagc aagttcgcaa tgtgagcgcg
     2161 tctctctggt cttgcagatt ggcgatgttt ttttccacgg tggtgatatc actttgtaac
     2221 tgcgcgcgtt gtaaatcggt atcgctttcc aaatttctca a
//
LOCUS       DAOSFC010000230         2258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_47229_length_2258_cov_5.590104, whole genome shotgun sequence.
ACCESSION   DAOSFC010000230 DAOSFC010000000
VERSION     DAOSFC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47229_length_2258_cov_5.590104"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PK791_05860"
     CDS             complement(<1..1222)
                     /locus_tag="PK791_05860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOH92871.1"
                     /translation="MPNSVTIEKLVYGGDGLGRLSDGRAVFVPFVLPDEEISLEITEE
                     KARFTRGTALSWHKSSPHRIPARCEYFTVCGGCHYQHMPYQKQLETKLETVRDQLSRI
                     AGINPQALSHITQSSQSWHYRNQIQCHVTPDGELGFMRADNTGILPIKHCEIAMPGLN
                     ALLNTINLDPESGIKRVTFREDSDGECFVLLEGEDPLPPELTVELEVSAGYMDAEQNY
                     HHLAGTDQLHYRILDKDFTLSPGSFFQINNAVCEELVKHVIGLTKSITTHKVLELYSG
                     AGLFSAFLAAMVSQLIAIESTPSACYDFANNLDAFDNVSLYEGAVEDILPGLLSLLQP
                     EAPDLVLLDPPRAGLHIKALDALADLAPENIIYVSCDPSTLARDLKRLSNKGYQVQTI
                     HAFDMFPQTYHVETV"
     gene            1648..>2258
                     /locus_tag="PK791_05865"
     CDS             1648..>2258
                     /locus_tag="PK791_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH92872.1"
                     /translation="MTTTDKPIRKKKKRLSGWWIVLIIAVLLAGFFTVNNYIKRQRAE
                     QTISQLKTVPYQRDTLVSTISGSGTVRARQSAVLVWQSSAYVGEVLVEVGDEVKANAT
                     LMRLDKNRLPVDILQAELNKLDIESKLENLESDTDLQRAQLQSDIATVEKNIRNLQDQ
                     KDALTLRVCEEYRITNLQNAYDNALANYEDWPTQARWIAVENAR"
BASE COUNT          553 a          494 c          692 g          519 t
ORIGIN      
        1 caaccgtctc aacatgatag gtctggggga acatgtcgaa agcgtggatg gtttggactt
       61 ggtagccttt gttgctgagg cgtttgaggt cgcgggcgag ggtgctgggg tcgcaactga
      121 catatattat gttttcgggt gccaggtccg cgagcgcgtc gagcgctttg atgtgcagac
      181 ccgcgcgggg tgggtcgagc aggaccaggt cgggtgcttc gggctgtagc agggacagca
      241 agccgggcag gatgtcctca accgcgcctt cgtacaggct gacgttgtcg aaggcatcca
      301 ggttgttggc aaagtcgtaa caggcggatg gcgtggattc aatggcgatg agttggctaa
      361 ccatggctgc caaaaacgcg ctgaacaaac ccgcgccgct gtagagttcc agcactttgt
      421 gggtcgtgat gcttttcgtc aggccgatga cgtgtttcac gagttcttca cagacggcat
      481 tgttgatctg aaaaaaagag cccggcgaga gcgtaaagtc tttatccaaa atgcgatagt
      541 gcagctggtc ggtgccggcg agatgatggt agttttgttc agcgtccatg taaccagcgg
      601 agacttcaag ctccacggta agttccgggg gcagggggtc ttcgccttca aggagcacaa
      661 aacattcgcc gtcgctgtct tcgcggaagg tgacgcgctt aatgccactt tcggggtcaa
      721 ggttgatggt atttagcaga gcattgagcc cgggcatggc aatctcgcaa tgtttgatgg
      781 ggagaatgcc ggtgttgtcg gcgcgcataa agccaagttc gccatctggg gtgacatggc
      841 attgaatttg gttgcgatag tgccagcttt ggctgctttg ggtgatgtgg ctgagtgcct
      901 ggggattgat gccagcaatg cgactgagtt ggtcgcgaac ggtttcgagt ttggtttcga
      961 gctgcttttg gtagggcatg tgttggtaat ggcagccgcc acaaaccgtg aaatattcgc
     1021 atcgtgcggg aatgcgatgg ggtgagcttt tgtgccagct gagcgcggtg ccgcgggtga
     1081 aacgggcttt ttcttccgtt atctcaaggg agatttcttc atcgggcagg acgaaaggca
     1141 cgaaaacggc acggccgtct gaaaggcgac ctagtccatc gccaccgtag actagttttt
     1201 cgattgtgac tgagttgggc atagcactcc ttgatgcgct tgggcttttg caatgtttgt
     1261 gtttatttta cccgtgagaa tggaatatag gcgaaaatga taggtgtatt gcggtgcgaa
     1321 gtggcgcttt tacgtggtca ggagaccacg tcagaacggg gtggaaaacg tcggcgagac
     1381 gcctccgcga acggatctta gtgatttggt ctttttacgt ggtcgggaga ccacgttaga
     1441 acgggttgag agaccacgtc agaacgagat gagagaccat gtcggaaagt aacggggatg
     1501 tacgtcgctg ggaagccatg catcagtgga aaaaagcgca gatcttatgg gcactaagcg
     1561 gtatcatgta aaattgaatt cgtaacggat taatagcaat ttaatagtta tcgcatatcc
     1621 ttaaggaata cgattgcgga ggcacctatg acgacaacag ataaacctat caggaaaaag
     1681 aaaaagcgac taagcggatg gtggatagtc ctcattatcg ccgtattgct ggcgggcttt
     1741 ttcactgtga ataactacat aaaacgacaa cgcgcggaac aaaccatcag tcagctgaaa
     1801 acagttccct atcaacggga cacactcgtt tccaccatca gcggttccgg aacagtacgc
     1861 gccaggcaat cggcggtgct ggtctggcag tccagcgctt atgtaggcga ggtcttggtt
     1921 gaagtcggcg atgaggtcaa ggcgaatgca acactcatgc gtttggataa aaaccgcctg
     1981 ccagtcgata ttttgcaagc tgaactcaac aaactggata ttgaaagtaa gctggagaat
     2041 ttggaaagcg ataccgattt acaacgggcg caattacaga gcgatatcgc gaccgtggaa
     2101 aagaacatcc gcaatctgca agaccaaaaa gacgcgctga ccctaagggt ttgcgaagaa
     2161 tatcgcatca cgaacctgca aaacgcgtac gacaacgcgc tggccaatta cgaagactgg
     2221 ccaacgcaag ccagatggat agcggtggag aacgcccg
//
LOCUS       DAOSFC010000231         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_47320_length_2255_cov_4.042727, whole genome shotgun sequence.
ACCESSION   DAOSFC010000231 DAOSFC010000000
VERSION     DAOSFC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47320_length_2255_cov_4.042727"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PK791_05870"
     CDS             complement(<1..360)
                     /locus_tag="PK791_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOH92873.1"
                     /translation="MKVILTGSIAFDYLMKFPGLFKDTILPEHIEKISLSFLVNEMIR
                     QDGGVGGNIAYSLAMLGGKAYMFSTAGQDFGEYAQRLNDVGVDTTGVQIIPDRFTASF
                     FTTTDTSNAQIASFYTGA"
     gene            complement(369..1415)
                     /gene="mtnA"
                     /locus_tag="PK791_05875"
     CDS             complement(369..1415)
                     /gene="mtnA"
                     /locus_tag="PK791_05875"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOH92874.1"
                     /translation="MRTIVWNEKQKSVEIIDQNALPLEYKTVNLLTYQDVVFAIQHMT
                     VRGAPAIGVTGAYGMALAAMHSTKTDLDAMKAELHQVAQEIGNARPTAVNLWNLINHT
                     LAYINQFDGHVDKLKDHLLNLAHRLAEEDVETNVRLSKYGAELINDGDHIVHHCNTGA
                     LATVDYGTALGVIRMAHEQGKKIHVYVDETRPRLQGARLTAWELKQYGIPYDIITDSS
                     SGHLMQMGLVDKVFFGADRVAMNGDVANKIGSYMLALAAVDNKIPVYSVFPVSTVDAE
                     ISSGKEIEIEQRDSREVLDITLGKEIVVPSGATAVNYAFDVTPNRLLTGLVTEHGVLY
                     PPFFEKLDKIIREE"
     gene            complement(1515..>2255)
                     /gene="yqeC"
                     /locus_tag="PK791_05880"
     CDS             complement(1515..>2255)
                     /gene="yqeC"
                     /locus_tag="PK791_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HOH92875.1"
                     /translation="MKINEALRIGNKSAVAFVGAGGKTTAMFNLAREFDFPSIITTTT
                     NMGLEQITLGDKHINLNGDLPDGSDFSDEKVTVVTDRMNANRSRWQGLDDLQISRLRN
                     VAGVLSRALLIEADGAKTLPLKAPGEDEPIIPDFVDQVIVIAGLGAVGNFLDEGNVFR
                     PALYSELSGEEQNKPVSLESIARMLVNPMGGLKGIPENAKRTAILNHADLVPLNDSEL
                     AMFRNYLIKHYHEAYIGSLHEGWLLGLY"
BASE COUNT          600 a          582 c          479 g          594 t
ORIGIN      
        1 cgcgccagta tagaatgacg cgatttgggc gttgcttgtg tcggttgtcg taaagaatga
       61 agctgtaaat ctatcaggga taatctgcac accagtagta tccacgccca catcattaag
      121 ccgttgtgcg tattcgccaa agtcctgccc ggcagttgag aacatataag ccttgccacc
      181 caacattgcc agactgtaag caatatttcc accaacgcca ccatcctggc gaatcatctc
      241 gttcaccaaa aacgacaggc tgattttctc aatgtgttcg gggagtatcg tgtccttaaa
      301 cagtcctgga aatttcatta aataatcaaa ggcgattgag cccgtaagaa tgactttcat
      361 atttgttttt attcctcgcg tatgatttta tccaactttt caaaaaacgg gggatacaaa
      421 acgccatgct ccgtcaccag gccggttaaa agccggttcg gcgtgacatc aaaggcataa
      481 ttgaccgcgg tcgcgccact gggaacgaca atttctttac ccaaggtgat atccaaaacc
      541 tcgcgtgaat cgcgttgttc aatttcaatt tccttaccgc tggaaatttc cgcgtcgact
      601 gtgctgacag gaaataccga gtaaaccgga atcttgttat ccaccgcggc aagggcaagc
      661 atataactgc cgatcttatt cgccacgtcg ccattcatcg cgacacggtc cgcgccgaaa
      721 aacaccttat ccaccaaacc catttgcatc agatgcccgg aagatgaatc ggtaataatg
      781 tcgtaaggaa tgccgtattg tttcaattcc caggcggtta aacgcgcgcc ctgcaagcgt
      841 ggtcgcgttt catccacata aacatggatt ttcttgccct gttcgtgtgc catgcggata
      901 acgcccaaag ctgtaccata atcaaccgtt gccagtgcgc cagtattgca atgatgcaca
      961 atatggtcgc catcattaat cagttcagcg ccatatttag acaaacgcac gttggtctca
     1021 acgtcttctt ccgccaggcg atgcgcgaga ttgagtaaat ggtcttttaa cttatccaca
     1081 tgaccgtcaa actgattgat atatgccagc gtgtgattga ttaaattcca aagattcaca
     1141 gcggtaggtc gggcattgcc aatttcctgg gcgacttgat gaagttccgc tttcattgcg
     1201 tccaaatcag ttttagttga atgcatcgct gccaaagcca tgccatacgc gccggttaca
     1261 ccgatggctg gcgcaccccg cacggtcatg tgctggatcg cgaaaaccac atcctggtat
     1321 gtcagcaaat ttacggtttt gtattcaagt ggcaaagcgt tttggtcaat aatctcgacg
     1381 gatttttgtt tttcattcca tacaattgtt cgcatatttc tctcacgtaa ataaagggct
     1441 tttcgaccct gtttttatcg ggttcagtct aacacaatcc aggttgaagt aaaagtgcct
     1501 ggcactaatg cagactaata taagcccaaa agccagcctt catgcaaaga gccgatataa
     1561 gcttcgtggt aatgcttgat aagataattc ctaaacattg ccagttcgct gtcatttaac
     1621 ggcaccaaat cagcgtgatt gagaatagct gtgcgtttgg cattttcagg aatacctttc
     1681 aacccaccca tcgggttcac caacattcgc gcgatgctct caagcgagac cggcttgttt
     1741 tgttcttcgc cactcagttc ggaataaagt gcgggacgga acacattgcc ctcgtccaaa
     1801 aagttaccca ccgcgcccag tccggcaata acaatcacct ggtcaacaaa atcgggaata
     1861 atcggttcat cttcacccgg cgctttcaag ggtaatgttt tcgcgccatc tgcctcaatt
     1921 aacaaagccc ggctaagaac accagccaca ttgcgcaaac gggaaatctg caaatcatcc
     1981 agaccctgcc aacgcgaacg gttggcgttc attcggtcgg tcaccacagt aaccttttca
     2041 tcgctaaaat cactgccatc gggtaaatca ccgttaagat tgatatgttt gtcacccagc
     2101 gtaatctgct caaggcccat attagttgtg gtagtgataa tcgaagggaa gtcaaattcc
     2161 cttgccaggt tgaacatcgc cgttgtttta ccaccagcac caacaaaggc caccgctgat
     2221 ttattgccta tgcgcaaagc ttcattaatt ttcat
//
LOCUS       DAOSFC010000232         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_47961_length_2235_cov_2.839450, whole genome shotgun sequence.
ACCESSION   DAOSFC010000232 DAOSFC010000000
VERSION     DAOSFC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47961_length_2235_cov_2.839450"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PK791_05885"
     CDS             complement(<1..934)
                     /locus_tag="PK791_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOH92876.1"
                     /translation="MKKLTKLILLLLMATLLLSACKGTVPVPENMPTKASGEETEEPV
                     KEQIVNPAEETEPSMKSAEDIFALTRTSTDPLTTDDDRMTCTPSEPIFAPLSAELRAQ
                     YQIPTLPEITEEDHVKGSEDAIMTVIFYTDFQCPYCALFASESHKLFEAFPDEVRLVY
                     RPLPLSFHNLAPLAAQAAEAAGLQGKFWEMHDHIFESQQEWNSLTDEGFTEWLNDAAE
                     EIGLDVEQFSADLVSEAIVEKIAKETEQQLGLGINATPSLFINEYPWQSSWSFETLSS
                     VIDVMKAEAELSYECPPFVIDPEKKYIATMETEKG"
     gene            complement(990..1613)
                     /gene="tmk"
                     /locus_tag="PK791_05890"
     CDS             complement(990..1613)
                     /gene="tmk"
                     /locus_tag="PK791_05890"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOH92877.1"
                     /translation="MFITLEGPEGSGKSSQIPALAQYLEAQGYRVLCTREPGGTSIGD
                     QIRAVLTSMDNPELLPRTEILLFLAARAQLVAQVIRPALREGKIVLCDRYGDSTLAYQ
                     GYGHGLDLKSLRAMLDFATEGLKPDLTLLLDLDIKTGLARKKSIDEWNRLDAYEVSFH
                     ERVRAGYLQLAEEEPTRWQRVDASMPKDEVQNLLRQIVLKRIQDKIE"
     gene            complement(1615..>2235)
                     /locus_tag="PK791_05895"
     CDS             complement(1615..>2235)
                     /locus_tag="PK791_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOH92878.1"
                     /translation="ASQSLRAGKNMENSPFQRRSANGDAQPRQKNRKERYEDFVDPED
                     PILNLEDSREKRIFVFGVARNRLLQAIKSMNAPVVIVREMKDADMFLTQRRYYRDRLQ
                     PIVDAEAKGLPIFVIRSNSMAQIEQFLADTFGLATPQQKREKDDEALAQVDIAVDLLQ
                     AGEPFVDLQPTFAHIRKRQHELAREYGLVSQSFGKDPNRYVRIYRD"
BASE COUNT          547 a          552 c          528 g          608 t
ORIGIN      
        1 cgcccttttc agtttccatt gtggcaatgt attttttctc tggatcgata acaaaaggtg
       61 gacattcata gctaagctct gcttcagctt tcatgacatc aatgaccgag ctaagagtct
      121 caaaactcca gcttgattgc cagggatatt cgttgataaa taaagaagga gtcgcgttga
      181 tacctaaacc taactgttgt tcagtttctt tcgcaatctt ctcaacaata gcctcgctaa
      241 cgagatcagc actgaattgt tccacatcca aaccgatttc ttcagcagca tcgttcagcc
      301 actcagtaaa gccttcatcg gtcagactgt tccactcttg ttgcgattca aagatatggt
      361 catgcatttc ccagaacttg ccttgcaggc cagcagcttc ggcagcctgc gccgcaaggg
      421 gtgctaaatt gtggaaagac aagggtaaag gacgataaac caaacgcact tcatcaggaa
      481 aagcttcaaa cagtttatgg gattcactgg caaaaagcgc gcagtaagga cactgaaagt
      541 ctgtatagaa aatgaccgtc attatcgcgt cttccgatcc ctttacatga tcctcctcgg
      601 tgatttctgg aagagttggg atttgatatt gcgcgcgaag ttcagctgat aagggcgcaa
      661 aaatcggctc agaaggcgtg caagtcatgc gatcatcatc ggtagtaagc gggtcggtgc
      721 ttgtgcgggt aagcgcgaag atatcctcag ctgatttcat gcttggttct gtttcctcag
      781 cggggttaac gatttgttcc ttaaccggtt cttcggtctc ttctccagaa gctttggtgg
      841 gcatattctc cggaacgggc actgtgcctt tgcaagcgct cagcaataag gtagccatca
      901 agagaagcaa gattagtttt gttagttttt tcatatgatc ctttcatccg tccaggcata
      961 cgcctttgac ggttcattac aatagttcct tactctattt tatcctgtat tcttttcaac
     1021 acaatctggc gcaacaagtt ttgcacctca tcttttggca ttgaagcgtc aacccgttgc
     1081 cagcgcgtgg gttcttcttc agcgagttgc aaatagccag cccgcacgcg ttcatggaac
     1141 gagacctcat acgcgtcaag acggttccat tcatcaatgc ttttcttacg cgccaaacct
     1201 gttttgatat ccaaatccaa gagcagggtc aagtccggtt tgagtccttc ggtggcaaag
     1261 tcgagcattg cccgtaaact cttcaaatcc aagccatgtc cataaccctg ataagctaag
     1321 gttgagtcgc catagcggtc gcaaaggaca atttttccct cgcgcaaagc ggggcggata
     1381 acctgcgcaa ccaactgcgc gcgggctgcc aaaaaaagca gaatctcagt gcggggcagg
     1441 agttccgggt tatccatgct ggttaatacc gcgcggattt ggtcaccaat agaagtgcca
     1501 ccaggttcac gcgtgcaaag cacacgataa ccttgcgctt caagatattg cgctaacgcg
     1561 gggatttggc tgcttttgcc actgccttcg ggtccttcaa gggttataaa catattaatc
     1621 tcgatatatt ctcacgtaac ggttggggtc ttttccaaaa gactgcgaaa ccaagccata
     1681 ttctctggcc aattcgtgtt gtcttttgcg gatatgcgcg aaggttggtt gtaaatcgac
     1741 aaaaggctca ccagcctgga gcaaatcaac agcgatatca acttgcgcca gggcttcatc
     1801 atctttttca cgtttttgct gcggtgtggc aagtccaaag gtatcagcaa ggaattgctc
     1861 aatctgcgcc attgagtttg agcgaatcac aaaaataggc aaacctttcg cttcggcgtc
     1921 cacgatgggc tgcaaacggt cacgatagta gcgacgctgg gtaaggaaca tatcagcgtc
     1981 tttcatctct cgcacaatga ctaccggcgc gttcatgctt ttaatcgctt gcaacaagcg
     2041 gttgcgtgcc acaccgaaga caaatatgcg tttttcacga ctgtcttcca ggttcaggat
     2101 gggatcctcg gggtccacaa agtcttcgta gcgttctttg cggtttttct gccgtggctg
     2161 tgcgtcccca ttagcagacc gacgctggaa aggggaattc tccatgtttt tgccagctct
     2221 caggctttgg ctggc
//
LOCUS       DAOSFC010000233         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_48938_length_2204_cov_6.034435, whole genome shotgun sequence.
ACCESSION   DAOSFC010000233 DAOSFC010000000
VERSION     DAOSFC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48938_length_2204_cov_6.034435"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PK791_05900"
     CDS             <1..372
                     /locus_tag="PK791_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH92879.1"
                     /translation="SLPGTPFGLAQSEYSPAREMIAANAYLALATDLNPGTTWNESMQ
                     FMQALATRYMKLTPSQALAASTINSAKAVLRDATIGSIEAGKQADILILSVPDYRILT
                     YRYGTNMVANVIKKGELYPQK"
     gene            372..1025
                     /locus_tag="PK791_05905"
     CDS             372..1025
                     /locus_tag="PK791_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH92880.1"
                     /translation="MWDFYPDIEDARAWYASAGLAHDFEHALRVLNLAMKIAENEAAD
                     LEIVYVAALLHDSRGATPGGEDGARMGHHLASAQFAGEILAQAGWKKERIEAVQHCIR
                     AHRYRHDGEQSQSLEAQCVFDADKLDVLGAVGVARTIAYATHAGQPFYARPSEQFLNT
                     GERLPDEPHSAYHEYLFKLRHVKDRLYTSTGKQIAAQREAILHKFFNQLQAECEGEA"
     gene            1211..2029
                     /locus_tag="PK791_05910"
     CDS             1211..2029
                     /locus_tag="PK791_05910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOH92881.1"
                     /translation="MKPQLEQIKSWARTVGQMAIEMRKEDLDIEQKGLADLVTRADKA
                     IEAYLLDQVATNFPKHTVLGEETGMHQKDGAHRWYIDPIDGTLNYAHGLPEYSISIAY
                     AFEGELQLGVIYCPEMDELFSAEKGKGAWLNGKPIQVSKIDKLIDSLLITGFRHSLID
                     TPRSNMDYFIHMARHSQTIRRLGSAALDLAYVAAGRAEGFWEIALNPWDVAAGILLVR
                     EAGGKVEGLFGEANLLEGSVDILAANPVIFPQMRALLIEVRNKKDNNDAYEWQQ"
     gene            2007..>2204
                     /locus_tag="PK791_05915"
     CDS             2007..>2204
                     /locus_tag="PK791_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92882.1"
                     /translation="MHTSGNNNLESWKYDQVDERYISPAPKTNFWPALIGVLIALVVY
                     LVGMLFLKTNVKISPSLFSLTD"
BASE COUNT          582 a          562 c          537 g          523 t
ORIGIN      
        1 tcacttccgg gcacaccttt tgggctggcc cagtccgaat actcacctgc ccgcgaaatg
       61 attgcggcta atgcttatct tgccctggcg actgacctga accccggcac aacctggaat
      121 gaatccatgc agtttatgca agctctggca acacgctata tgaaactcac acccagccag
      181 gctttggctg ccagcacaat caactctgcc aaagcggttt tacgcgatgc cacaatcggc
      241 tcgattgaag ccggtaaaca agccgatatt ttgattctaa gcgtccccga ctaccgcatt
      301 ttgacctatc gctacggtac caacatggtc gcgaacgtta tcaaaaaagg cgagctatat
      361 ccacaaaaat aatgtgggac ttttatcctg atattgaaga tgcccgtgcc tggtatgcca
      421 gcgcgggact ggcgcacgat tttgagcacg ccttgcgcgt gcttaatctt gcaatgaaaa
      481 tcgctgaaaa cgaagctgct gaccttgaga ttgtgtatgt cgccgcgctt ttgcatgatt
      541 cgcgcggcgc gacaccgggc ggggaagatg gcgcgcgcat ggggcaccat ttggcatccg
      601 cgcagtttgc gggcgagatt ttagcacaag ccggctggaa aaaagagcgc attgaggcgg
      661 tgcaacactg tattcgtgcc catcgctatc ggcatgatgg cgagcaatcc caaagccttg
      721 aggcgcaatg cgttttcgac gccgataaac tggacgttct cggcgcggtt ggtgtggcgc
      781 gtaccatcgc ctatgccacc cacgccggtc aaccgtttta cgccaggcct tcggagcagt
      841 ttttgaacac cggcgaaaga ctccccgatg aacctcatag cgcctatcac gaatatcttt
      901 tcaaacttcg ccacgttaaa gaccgtcttt acacttcaac gggcaaacaa attgctgctc
      961 aacgagaggc cattttacat aaatttttca atcaattaca ggcggagtgc gaaggcgaag
     1021 cgtagtaatc cccaataggc cagtctttta tccacagatt tttaaaactt atccacagtt
     1081 atcagttttc ttccggttcc agcagcaatt tttatgtgga taaaagtgaa aactttacag
     1141 acggcaatcg ctctgatggg ctaataattg atacaataga gagaccatac tatacaaacc
     1201 aaggacgtgt atgaaaccgc aattagagca aataaaatca tgggcgcgaa ctgtcggaca
     1261 gatggccatc gaaatgcgca aggaagacct ggatatcgaa caaaaagggc ttgccgacct
     1321 ggtgacccgt gccgataagg cgattgaagc ttacttgcta gaccaggtgg caacaaactt
     1381 tccaaagcac actgttttag gtgaagaaac cggcatgcac caaaaggatg gcgcgcaccg
     1441 ctggtatatc gaccccatcg acggcacctt aaactacgcg catggcttgc cagagtatag
     1501 catctctatt gcctatgcct ttgaaggcga acttcagctg ggcgtcattt attgccccga
     1561 gatggacgaa ctgttcagcg cggaaaaagg caaaggcgcc tggctcaatg gcaagccaat
     1621 ccaagtctca aaaatcgaca agcttattga ttcgctttta attaccggtt tcagacattc
     1681 cttaattgac acaccacgct ccaacatgga ttactttatc catatggcgc gtcacagcca
     1741 aaccattcgg cgtttgggct cggcagcgct ggacctggct tatgtcgccg ccggacgcgc
     1801 ggaaggcttt tgggaaatcg ccctaaaccc ctgggatgtc gcggccggta tcctgctcgt
     1861 gcgcgaagcg ggtggtaaag tggaaggact ctttggcgaa gcgaatttac tggaaggcag
     1921 tgtggacatt ttggccgcca atccggtcat tttcccccaa atgcgcgcct tgctcattga
     1981 agtcaggaat aagaaggaca ataacgatgc atacgagtgg caacaataac ttagaatctt
     2041 ggaaatacga tcaggttgat gaaagatata tatcaccagc cccaaaaacc aacttttggc
     2101 cagctttgat aggtgtttta attgccctgg tggtttattt agtgggtatg ctattcttga
     2161 aaaccaatgt gaaaatttct ccatctttgt tcagtctcac agac
//
LOCUS       DAOSFC010000234         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_49879_length_2176_cov_3.798208, whole genome shotgun sequence.
ACCESSION   DAOSFC010000234 DAOSFC010000000
VERSION     DAOSFC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49879_length_2176_cov_3.798208"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PK791_05920"
     CDS             complement(<1..1222)
                     /locus_tag="PK791_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH92883.1"
                     /translation="MTEFRIKSHPIIETTEDANILFFWQSKPFYARPNEMLSSALMAN
                     GVSVFSHHPVDGSPQGIFCANGQCSQCMVIADGIPVKSCMTPVKAGMCVEPAEGLPTL
                     PQVSDNYSFVEPNIPVTKTDVLIIGAGPAGLSAAIELGNLGIDCLLIDDKDRLGGKLV
                     LQTHRFFGSTEAVFAGSRGTEIAKKLESSVRALPSVKIWTRATAVGVFSDKVVGVFRD
                     DQKYELVKPKVLLVATGAREKSLAFPGNTLPGIYGAGAFQTLVNRDLVKPCKNLFVIG
                     GGNVGLIAAYHAIQAGIHVAGLVEAAPKCGGYKVHMDKLVRTGVPIYTSHTVLKAIGD
                     EHVEQIIIAQVDKNFQPIKGTEKLYDCDTVLIAVGLDPVNEFYRRAKQLGFNVHAAGD
                     AKAIAEASAAIFSGK"
     gene            complement(1280..2137)
                     /locus_tag="PK791_05925"
     CDS             complement(1280..2137)
                     /locus_tag="PK791_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOH92884.1"
                     /translation="MEINKLEPTGETLDKLKEDAIVWMANALKDFSPATEQVYLTATV
                     GLYEYIAGENLANINLPRVRLLIKQRSRRPGIRLPQFPKNDIERILIYAQDLHNAAHK
                     DDNARFIAYRDRAFLITLADTGLRVHEACNLRRGDIDWLESRAIIIGKGNKEAVTRFS
                     KRALKACKDYLGQRAVLDGNSGRPLASLPLFARHDRGAGKKVKPISTTTGREIVNQAV
                     FNAIGPEAVGTITPHSFRHYFVTTILISSGNLKLAQELARHTNIAVTQRYSHLNDDEL
                     DQKYQEIFD"
BASE COUNT          626 a          483 c          451 g          616 t
ORIGIN      
        1 tttttccgga gaatatcgca gcactggctt cagcaatcgc tttggcatca ccggcggcat
       61 gaacgttaaa accaagttgt ttggctcttc gataaaattc gttaactgga tcaagaccaa
      121 cagcgataag aaccgtatca caatcatata gtttttcggt tcctttgatg ggttggaagt
      181 ttttatcaac ctgggcgata ataatctgtt caacgtgttc atcaccgatg gctttcaata
      241 ctgtgtgtga agtataaata ggcacaccag tccgaaccag tttatccatg tgaaccttgt
      301 atccgccaca ttttggtgcg gcttcaacca agccagctac atgaattccg gcttgtatgg
      361 catggtaagc ggcaataagt ccaacgttac cgcctccgat tacaaacaag ttcttacatg
      421 gtttgactaa atcgcgattg accagtgttt gaaatgctcc ggcaccataa atccctggca
      481 atgtgttacc ggggaatgca agactttttt cgcgcgcgcc tgtagctaca agcaaaacct
      541 ttggctttac taattcatat ttttgatcat cgcgaaaaac accaacaact ttgtcagaaa
      601 acacaccaac tgcggtagcg cgcgtccaaa tttttacaga aggcaaggca cgaacgcttg
      661 attcaagctt tttggcgatc tctgtaccgc gactgccagc aaaaacggct tcagtagacc
      721 caaaaaaacg atgagtctga agtaccaact taccacccaa gcgatcttta tcatcaatta
      781 atagacaatc tatgccaagg ttaccaagtt caatagcagc tgaaagtcct gcagggccag
      841 cacctatgat aagaacatca gttttagtaa caggtatatt gggttcaaca aagctataat
      901 tatcagatac ctggggtagg gtaggcaatc cttcagccgg ttctacacac ataccagctt
      961 ttacgggagt catacaagat tttacgggaa taccatccgc tatcaccata cattgcgaac
     1021 attgtccgtt ggcacaaaag ataccctgtg gagagccatc aaccggatga tgactgaaca
     1081 cactcacgcc attagccatc aaagcggatg aaagcatttc attgggtctg gcgtaaaatg
     1141 gttttgattg ccaaaagaag agaatgttgg catcttcagt tgtttcaata ataggatggg
     1201 atttaatccg aaattcagtc attaattgcc tctaaattgt tggtgttcat cgatgaacaa
     1261 tgataccacc ttggtcaact taatcgaata tttcttgata tttttggtca agttcatcat
     1321 cattaagatg agaatagcgc tgtgttaccg cgatattcgt atgtcgggca agttcctggg
     1381 cgagttttaa atttcctgaa ctgataagaa tagtcgtcac aaaatagtgt ctaaaactgt
     1441 gtggagtgat tgtaccaact gcttcaggac caattgcatt gaaaacagcc tgatttacaa
     1501 tttcacgacc cgtggttgtt gatatcggtt ttactttttt accggcgcca cgatcatgtc
     1561 tggcaaacaa aggtaaggat gctaaaggtc tgccactgtt accatccaaa actgcgcgtt
     1621 ggccaaggta atctttacag gcttttaaag cacgtttaga aaatcttgtt accgcttctt
     1681 tattgccttt accaatgatg attgcacggc tttccagcca gtcaatatca ccacgccgca
     1741 aattgcaagc ttcgtgaaca cgcagacccg tatcggctag tgtaatgaga aacgcacggt
     1801 cccgataagc aataaaacgc gcattgtcat ctttatgtgc cgcattatgc aggtcttgcg
     1861 catagataag aattctctca atgtcattct ttggaaattg cggtaagcga atgcctgggc
     1921 gtctggaacg ctgtttgata agcagtctta cacgtggcaa attgatgttg gctaaatttt
     1981 caccggcaat atactcataa agaccaacag ttgccgttaa atagacttgc tctgtagcgg
     2041 gtgaaaagtc cttaagcgca tttgccatcc agacaatagc atcttcttta agtttgtcta
     2101 aagtctctcc ggtaggttca agtttattaa tttccaaaac ttcgctaaac atggtcatcg
     2161 cattcctgta agtacg
//
LOCUS       DAOSFC010000235         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_50054_length_2171_cov_7.985822, whole genome shotgun sequence.
ACCESSION   DAOSFC010000235 DAOSFC010000000
VERSION     DAOSFC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50054_length_2171_cov_7.985822"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /gene="pstC"
                     /locus_tag="PK791_05930"
     CDS             complement(<1..739)
                     /gene="pstC"
                     /locus_tag="PK791_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOH92885.1"
                     /translation="MSNSKAKIMSRPETREKLMKVIFLLAALSSILALFLIMYFIFGA
                     GIPFIKEYGLKEFLFGQVWSPVNKPQQYGIWPMIVGSVIITIGAALVGTPIGILCAVY
                     LSFYAKGKLKYFFQSAVSLMASVPSIIYGFFALQLMVPISRALFGGTGMNIITASVLL
                     GIMILPTIVSISESSLNAVPNRYYQGSVALGATKEISIFKTMIPAAKSGILSSVILGI
                     GRAIGETMAVYMVAGNQPRLPKSLTSGV"
     gene            complement(729..1646)
                     /locus_tag="PK791_05935"
     CDS             complement(729..1646)
                     /locus_tag="PK791_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH92886.1"
                     /translation="MKKHTTIILVLVALMLAVTACGTKSTPTTSAPKATEAPKVAEPT
                     TVKPVEIIVVSREEGSGTRGAFVELTGVEAKNEAGDKVDNTYIEAVIQNSTNGVMMTV
                     ANEANAIGYISLGSLNDTVKAVQIDGVEATAENVKNGSYKIARPFNIAYKAGLDEASQ
                     DLLDFILSEEGQKIVEERGYISSEAGAPFAGGKAEGTVVVAGSTSVTPVMEKLVEAYN
                     AINPNVKVEIQATGSSAGMQSTMEGVSQLGMASRELKDEEKAELEYKVIAIDGIAVIV
                     HKTNPVKSITMEQVRQIFVGELTDWNLVK"
     gene            complement(1863..>2171)
                     /locus_tag="PK791_05940"
     CDS             complement(1863..>2171)
                     /locus_tag="PK791_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92887.1"
                     /translation="AERDTTTRTMMSVDAPDYASVIQRNALVYALYFANDEIVDTQAL
                     CDMDVTYLYSAKNTPFTNMDFNIEALKASPGVQLIYDQNGIQIFDLCTKTTELDTLQD
                     "
BASE COUNT          576 a          499 c          486 g          610 t
ORIGIN      
        1 gcacgccact ggtcagactt tttggcaagc gcggctggtt accagcaacc atataaaccg
       61 ccatcgtctc gccaatcgcg cgcccaatgc ccaaaatgac cgaactcaat atacccgatt
      121 tcgcagcggg tatcatcgtc ttgaaaatgc tgatttcttt ggtagctcct aaagccacac
      181 ttccctgata atagcgattg ggcacagcgt tgagactgga ctcggaaatg ctgacaattg
      241 tcggcagaat catgattccc agtagcaccg aagcggtaat gatattcata cccgttccgc
      301 caaacagtgc cctgctgatg ggtaccatca gctgcaacgc gaagaagcca tagatgatag
      361 aaggcacgct tgccatcaaa cttaccgctg attggaaaaa gtatttaagc tttcctttgg
      421 cataaaacga taggtacacc gcgcatagta tgccaatcgg tgtacctacc agagcagctc
      481 caatcgtgat gataacagac ccaacaatca taggccaaat gccatattgt tgaggcttat
      541 tcaccggact ccacacttgc ccaaacaaaa attccttcag gccgtattcc ttaataaaag
      601 gaataccggc accgaagata aagtacataa taagaaatag cgctaagatt gaacttagcg
      661 ctgccaacag gaaaatgact ttcattaatt tttcgcgtgt ttccggtcgg ctcataattt
      721 tggctttact atttgacaag gttccagtcc gttagttcgc caacgaaaat ttgtctgacc
      781 tgttccatag tgatggactt gacagggttg gttttgtgaa cgatcactgc gataccgtcg
      841 atagcaatca ctttgtattc aagttcagct ttttcttcgt ctttcaactc acgtgaagcc
      901 ataccgagtt ggctaacacc ttccatggtg ctttgcatac cagcggatga accagtggcc
      961 tgaatttcga ccttgacgtt tggattgata gcattgtagg cttcaaccag tttttccata
     1021 acgggggtaa cagaagttga accagccaca acaacggtac cttcagcctt gccacccgcg
     1081 aaaggagcac cagcttcgct ggagatgtaa ccgcgttctt caacgatttt ttgaccttct
     1141 tcactcagga tgaagtcaag caaatcttgt gaagcttcat caagaccagc tttgtaagca
     1201 atattgaaag gacgggcaat cttgtagcta ccgtttttca cgttttcggc agtagcttca
     1261 acgccgtcaa tctgcactgc tttgacggtg tcattgagcg aaccgagaga gatgtaacca
     1321 atcgcgttgg cttcgttggc aacggtcatc atcacaccat tggtgctatt ttggatgaca
     1381 gcttcgatgt aggtgttgtc aactttgtcg ccagcttcgt ttttagcttc aacgccggtc
     1441 agttcaacaa aagcgccacg ggtaccagag ccttcctcgc gggaaacgac gataatttca
     1501 acgggtttaa ccgttgttgg ttctgcaact ttgggggctt cggttgcctt gggggcggaa
     1561 gtagtggggg tgctcttagt gccacatgcg gtcactgcca acatgagagc aacaagcaca
     1621 agaataatag tagtatgttt cttcattttt ttcttctcct atttttcatt tacccacata
     1681 ttgtagatgt ctggtatttc caatgtatct tgataatgta aagttcttgt aaagtctatg
     1741 ggacgctcat taaagtcatg ggtcaatgat aattcttgtt ttctctcata gcggagtaaa
     1801 aacagtgtaa tgacgtttgt tgcaaatcag aaaatatttc aacaactggg aaatatcgtg
     1861 gtttagtcct gcaatgtatc gagttcagtc gttttggtgc aaaggtcaaa aatttgaatg
     1921 ccgttttggt catagataag ctgtacacct ggactggctt ttaaggcttc aatattaaaa
     1981 tccatgttcg tgaaaggcgt atttttagca ctataaaggt acgtcacgtc catgtcgcaa
     2041 agagcctggg tgtccacaat ttcatcgttg gcaaagtaga gcgcgtaaac caaagcattg
     2101 cgttgtatga cactagcata atctggcgcg tcaaccgaca tcatggtgcg cgttgtcgta
     2161 tcgcgttctg c
//
LOCUS       DAOSFC010000236         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_50415_length_2160_cov_5.742043, whole genome shotgun sequence.
ACCESSION   DAOSFC010000236 DAOSFC010000000
VERSION     DAOSFC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50415_length_2160_cov_5.742043"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK791_05945"
     CDS             complement(<1..231)
                     /locus_tag="PK791_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH92888.1"
                     /translation="MPKPTFYNLSAARRARIEAVAIDEFSKNGLKSAVLNNIVAKSGI
                     AKGSFYQYFENIEDLYQHILSLVQKRKTDVASS"
     gene            451..984
                     /locus_tag="PK791_05950"
     CDS             451..984
                     /locus_tag="PK791_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH92889.1"
                     /translation="MSKSKTKSQTGEKAHSFSELYRRHVNQVYYYIYSRVQNTADAED
                     ICSETFITALETRHKLRDESRFKPWLFTIARNKVNDYFRRVYRRPKSELTPKTVDKIS
                     LIDYHDGLDRDRMLALEQMLNALTPIEQDYIRLRLIADLPFADIAQVLRQSENKVKKA
                     YYRLVERLKAQVEENND"
     gene            977..>2160
                     /locus_tag="PK791_05955"
     CDS             977..>2160
                     /locus_tag="PK791_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92890.1"
                     /translation="MTKLSDFEKELKSVIKIEEPDGAYKNRLQTCLRERELNGVTVPQ
                     KANLRWVYVAIPLLIVMAVILIAGPNKVLAQIKSWLGFVPGVGIVEKDAEIWTLQEPV
                     VMTKDGVELQIKEAWFTPEKSVVNYAFFNVPNPNFYPGMVLEQCYLPAWLETEDGTRY
                     EEIKMGEFPGLPAGTAQVSLHVPCIWNTVLNNSAPQDWQANLNLMPAGDGLTIQDVHT
                     MADALPEPQVVPVEIQDPSALETRFEDLDNPIPLPKAEKTIENGVQIHNIIRDDDALV
                     LTGTFSGAVDLGDLYLQGEMKIEDADGNYVPYRIPENIRALIPYENYSYGMNWAIEMR
                     PGGFKAPFTIKQLASTAKRADDVFDFEIQQEDFDKPQKSGEEPIWLYAHDLSRSFGDC
                     FL"
BASE COUNT          627 a          457 c          510 g          566 t
ORIGIN      
        1 agacgacgcc acgtccgttt tgcgcttttg cacaaggctt aatatgtgct gataaaggtc
       61 ttcgatgttc tcaaaatact ggtaaaacga gcccttggcg atgcctgatt tggcaacaat
      121 attgttaagc accgctgatt taagaccgtt tttcgaaaac tcatcaatgg ctacggcttc
      181 aatgcgcgcc cgtcgcgccg cgctgaggtt gtaaaatgtt ggctttggca taattttttc
      241 ctttacgact gattagtcat attctataac ggcagcaaaa caatgtcaac atgatatgac
      301 agacttcacc gtaaatatac tgatgtttgt gaagctcttt tatgccatca tttttaatgg
      361 ttttggcacg aatcgccctt ccaaagcgac gtattatatg tagctacaat ttataatagg
      421 aaaaagccta ttgagacaac acatagaatg atgagcaaat cgaaaacaaa aagccaaact
      481 ggcgaaaagg cccacagttt ttctgaactt tatcgccggc acgtgaacca ggtctattac
      541 tacatctaca gccgtgttca aaatactgcc gatgctgaag atatttgctc agaaacattc
      601 attaccgcac ttgaaacgcg tcacaaattg cgcgatgaga gtcgctttaa gccctggctc
      661 ttcacaatcg cgcgaaacaa agttaacgac tactttcgca gagtctaccg gcgacctaag
      721 tcagaattga ccccaaaaac cgtcgataaa atcagtctaa tcgattatca cgatggtttg
      781 gaccgggatc gaatgctcgc cttggagcaa atgctaaatg ctctaactcc gattgaacaa
      841 gactatatcc gattgcgcct gatagcggat ttgcctttcg cggatatcgc gcaagtgttg
      901 cgccaaagtg aaaataaagt aaagaaagct tactacaggc tggtggagcg tctaaaagcc
      961 caggtagagg aaaacaatga ctaagttaag cgattttgaa aaagaactta aaagtgttat
     1021 caagattgag gaacccgatg gtgcttacaa aaaccgttta caaacatgcc tgcgcgaacg
     1081 ggaactcaat ggggtaacag tgcctcagaa agctaatctg cgatgggtct atgtcgcaat
     1141 cccgctgctt attgtcatgg ctgtgattct gattgcagga ccgaacaagg tgctggcgca
     1201 aattaaaagc tggctgggct ttgtgcctgg tgtgggtata gttgagaaag atgctgagat
     1261 ttggacactt caggaacccg ttgtgatgac gaaagatggt gtcgaattgc agattaaaga
     1321 agcctggttt acaccggaaa aatcggtggt taattacgct ttctttaacg tccctaaccc
     1381 gaatttctat ccaggcatgg ttttggaaca gtgctatttg ccggcctggc ttgaaactga
     1441 agatggcacc cgctatgaag aaattaaaat gggcgaattt ccggggcttc ctgctggaac
     1501 agcgcaagtc agcctgcatg tgccttgcat ttggaacacg gttttgaata acagcgcgcc
     1561 acaagactgg caagcaaatc tgaacctgat gccagctggt gatggcctca ccatacagga
     1621 cgtacatacg atggcagatg ctttgccgga accgcaggtg gtgcccgtag aaatccagga
     1681 cccaagtgct ttagaaacaa ggtttgaaga cttggataat cctatcccct tacccaaagc
     1741 agaaaaaact attgaaaacg gggtgcaaat ccataatatt attagagatg atgacgcgct
     1801 tgtcttgact ggtacattta gtggcgcggt tgatttaggt gatttgtact tacagggtga
     1861 gatgaaaatc gaagatgctg atggtaacta tgtgccttat cgtatcccgg agaatatcag
     1921 agcactcatc ccttacgaga attacagtta tggcatgaac tgggcaattg agatgcgtcc
     1981 gggtggtttc aaagcgcctt tcactattaa acagcttgct tctaccgcga aacgcgcgga
     2041 tgatgtcttt gactttgaaa ttcagcaaga agattttgac aagccacaaa aaagtggtga
     2101 agagccgatt tggctctatg ctcatgacct tagtcgcagc tttggggatt gttttttgaa
//
LOCUS       DAOSFC010000237         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_50418_length_2160_cov_4.228029, whole genome shotgun sequence.
ACCESSION   DAOSFC010000237 DAOSFC010000000
VERSION     DAOSFC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50418_length_2160_cov_4.228029"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PK791_05960"
     CDS             <1..472
                     /locus_tag="PK791_05960"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HOH92891.1"
                     /translation="LFGLVSVFAEPSVAILNTQIEEETSGSIRGKTVLWTLAIGVSLA
                     VTISILRILIPEFRITHALLPVLGLVIALCFVAPDIFIGIAVDAGGVASGTMAAAFIL
                     PYAQGIAKNTVGADPLIDGFGVITFISMIPILSMLVLGFIYRVKARKAEQNQSA"
     gene            500..1159
                     /locus_tag="PK791_05965"
     CDS             500..1159
                     /locus_tag="PK791_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92892.1"
                     /translation="MNKEQKPTVTEKPSDMLLACVVTRGKGSQLMNLLRREGASYAVS
                     TFAEGTIPSHILNMLGIAKTKKELVFAFINREIEETCYQSLAHEMKLDQTGHGVAFSI
                     PLCNHPEPPTDTKLAATFIIVDKGRGEDVLDLLDELGLRGGTLISAFGGASMTDIRFN
                     LPIQPEKELIFMVAPKEPLPNMLKTLDEKLSFSEPHQGIAITFAITRAIGLYSEEKKG
                     K"
     gene            1161..1448
                     /locus_tag="PK791_05970"
     CDS             1161..1448
                     /locus_tag="PK791_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92893.1"
                     /translation="MEKRMLMVSVPLGKAERVVQLANKAGARGATILHTREALPEPKS
                     FQLEPQRDVVLIVTSVELQQGIEESIRAGFEQAIKVYCVPILNSVGLDHNS"
     gene            1566..>2160
                     /locus_tag="PK791_05975"
     CDS             1566..>2160
                     /locus_tag="PK791_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92894.1"
                     /translation="MRNKSFHKYSLIFLALLLLVSCARETPVPSELPAPQVSVSQHSE
                     TPTALPAEEQSTEITETAQTQNQVPEPPDNRTHYKLDLILDYYSKYGTVSETITYTNR
                     TGNTLDEILLLVPPRDFQNAIELQSLTGERVSSHHDEGIRTWVKLDPPLQAMESTSLT
                     ILFRLRFPANEGVFGYTNRQLNLSDWYPLIPPYDPETG"
BASE COUNT          581 a          581 c          470 g          528 t
ORIGIN      
        1 cctgtttggt ctggtttcgg tctttgccga accttcagtc gctatcttaa acacccagat
       61 tgaagaagaa acatccggtt ccatccgtgg aaaaaccgtc ctgtggacat tggccatcgg
      121 tgtatctctg gccgtcacca tctcgatttt acgcattctc attccggaat tccgtatcac
      181 ccacgccctg cttcccgtgc tgggtcttgt cattgccctc tgttttgttg cgcctgatat
      241 ctttatcggc atcgctgttg atgcaggagg tgtggcttca ggtacaatgg cagctgcctt
      301 catcctgccc tatgcacagg gcatcgccaa gaacacagtc ggcgctgacc cgctgattga
      361 cggttttggc gtaattacct ttatatcaat gatacctatt ctcagcatgc tggttctggg
      421 ctttatttac cgcgtgaaag cgcgcaaagc cgaacagaat cagtcagctt aagttttctt
      481 tagctaaaag tgagttaata tgaacaaaga acaaaaacca actgtaacag aaaaaccaag
      541 tgatatgcta ttagcgtgtg tcgtcacccg cggaaaaggc agccagctga tgaatctgct
      601 gcgacgcgaa ggcgccagct acgcagttag cacctttgcg gaaggtacga tccccagtca
      661 tattctcaat atgctcggca ttgctaaaac gaaaaaagaa cttgtttttg ctttcatcaa
      721 ccgggaaatt gaagaaactt gctaccaaag ccttgcccat gaaatgaaac ttgaccaaac
      781 cggacacggc gtggcttttt caatcccatt gtgtaatcac ccggaaccac cgacagacac
      841 aaaactggca gccaccttta tcatcgttga caagggcaga ggcgaagatg tgttggatct
      901 gcttgatgaa ctcggactgc gcggtggcac cttgataagc gcgtttggtg gcgcgtcgat
      961 gaccgatatt cgctttaatc tgcccatcca gccagaaaaa gaacttattt tcatggtcgc
     1021 ccccaaagaa ccgcttccaa atatgctcaa gaccctggat gagaaactgt cattcagcga
     1081 accccaccag ggcatcgcaa tcacctttgc catcacccgc gcgattggtc tttacagcga
     1141 ggaaaagaaa ggtaagtaat atggaaaaac gaatgttaat ggttagtgtt ccccttggca
     1201 aagctgaacg tgttgtgcag ctagctaata aagccggcgc gcgtggtgct accattttgc
     1261 acacccgcga agctcttccc gaaccaaaat cttttcagct ggaaccacaa cgcgatgtcg
     1321 ttctcattgt gaccagtgtc gaactccaac aaggtattga ggaaagcatc cgcgctggtt
     1381 ttgagcaagc cataaaagtc tattgcgtcc ctattttgaa ctcggtaggc ttagaccata
     1441 acagctaaac agtcagcaaa cgactaaaaa ctttagaagc tggcacacca ttagaaatat
     1501 ctgtatttcg aataggctgt gccagttttt gtatcttcaa aaagctggta aaatgttctg
     1561 taagtatgcg taacaaaagt ttccacaagt attcactcat tttcctggcc ttgcttctgc
     1621 ttgtcagttg tgccagggaa acacccgtgc caagcgaact gcctgccccg caagtatctg
     1681 ttagccaaca cagcgagacg ccaaccgcct tgcctgcgga agagcaaagc accgaaatca
     1741 ccgaaaccgc tcagacacaa aaccaagtcc ccgaaccacc tgataaccgc acacactata
     1801 agctcgacct tattctggat tattattcca aatacggcac ggtgagtgaa accattacct
     1861 acaccaatcg cacaggcaac accctggatg aaattttact cctggtgcca ccgcgcgatt
     1921 ttcaaaacgc aatcgaacta cagtctctca ctggcgaacg ggtaagtagc catcacgacg
     1981 aaggcattcg tacctgggtc aaattagacc ctccgctcca ggccatggaa agcacatccc
     2041 taaccattct gttccgcttg cgttttcctg ccaatgaagg cgtatttggc tacaccaacc
     2101 gccagctcaa cctgtctgac tggtatccac tcatcccgcc ttacgatccg gaaacaggct
//
LOCUS       DAOSFC010000238         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_50619_length_2154_cov_4.046689, whole genome shotgun sequence.
ACCESSION   DAOSFC010000238 DAOSFC010000000
VERSION     DAOSFC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50619_length_2154_cov_4.046689"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..2036
                     /gene="ligA"
                     /locus_tag="PK791_05980"
     CDS             33..2036
                     /gene="ligA"
                     /locus_tag="PK791_05980"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOH92895.1"
                     /translation="MEELKELREKINYHNYLYNTLDQPEISDYEYDQLFNRLKEIERN
                     HPELITPDSPTQKVGAKPSERFQKVNHPTQVLSLTNAFGSQETMDWYNRLLRLEPELE
                     KADFVLEPKLDGLTVVLHYHDGVFTLGATRGDGLVGEDITANLRTIPSLPLRIPVKPG
                     IDVPQEIVFRGEALILKEDFTKLNAELEAKGEKTYLNPRNTAAGSLRQLDPAITASRP
                     LKLFVYQILHASDTIPDTQEGILDYIKDLGLPCDSLRWHTDNIQDAISICEAQAEARH
                     NWPFEADGIVIKINNQTLYNRLGVIGKDARGAVAFKYPGEVVETSLLDIQNNVGRTGV
                     ITPLAILEPVMVGGVVVKQATLHNFDFIADKDIRIGDRVLLKRAGEVIPYIIASIPEK
                     RDGSQMPYIPPTTCPSCDTALIKDSEAVAWYCENVNCPAQLNRTIENYASRTAMDIAG
                     LGEKIVVQLTEAGLLKSIADLYILRKDELLKLDKFGELKASNLLNAIEASKSQSLQRL
                     IIGLGIHGIGEVSAGDLARHFKSLDALAQASPDDLQSIPGIGPNTAESIVHWFSQTQN
                     QATLQRLKSYGVWPVIQEQAKTESPKPLEGLTFVITGTLPNMSRDEAKQLIESNGGKT
                     TGSVSKNTSYLLLGENPGSKLDKALELGIPTISEEDLNNILNI"
BASE COUNT          635 a          524 c          441 g          554 t
ORIGIN      
        1 aactaccttt cgaccaacct cacccaaacc ttttggaaga attaaaagaa ctccgcgaaa
       61 agattaatta ccataactat ctttacaaca ccttagacca gcctgaaatt tctgactatg
      121 aatatgacca gctattcaat cgtctgaagg aaattgaacg caaccacccc gaactgataa
      181 ccccagactc gccaacccaa aaagttggcg caaagccatc cgaacgcttt caaaaggtca
      241 atcatccaac gcaggttctt agtttaacta acgcttttgg ttcgcaggaa acaatggact
      301 ggtacaaccg tttactccgt ctggaaccag agcttgaaaa ggctgatttc gttttagagc
      361 ccaaacttga cggactcacc gttgtcttgc attatcacga tggcgtcttc accctgggag
      421 ctacccgtgg tgatgggctt gtgggtgaag atatcactgc gaatttacgc accattccaa
      481 gcttgccgtt gcgcattcct gtaaaacctg gcattgatgt gccccaggaa attgttttcc
      541 gtggagaagc actcatcctg aaagaggatt tcacaaagct taatgctgaa cttgaagcta
      601 aaggcgaaaa aacctattta aacccacgta acacagcagc aggttcatta cgtcaactgg
      661 accccgcaat aaccgctagt cgcccattaa aactctttgt ttatcaaatt ctgcatgctt
      721 cggacaccat tccagatacg caagaaggca ttcttgacta cattaaagac cttggtttac
      781 cttgtgattc tcttcgttgg cataccgata acattcaaga cgctatttct atttgtgaag
      841 cacaagctga ggcacgccat aactggccct ttgaggcaga tggtattgtc atcaaaatca
      901 acaatcaaac tctttacaat cgccttgggg ttattggcaa agatgcccgc ggtgcggtgg
      961 cttttaaata ccctggcgaa gtcgttgaaa caagtttgtt ggatatccaa aataacgttg
     1021 ggcgtactgg tgtcattacg ccattagcga ttttagagcc tgtgatggtt ggcggtgtcg
     1081 ttgtaaaaca agccacctta cacaactttg atttcatcgc tgataaagat attcgcattg
     1141 gcgacagagt tttactcaag cgcgcgggtg aggtcattcc ctatatcatc gcgtccattc
     1201 ctgaaaaacg cgatggcagc cagatgccat atattccgcc aactacctgt ccaagttgtg
     1261 atacagcttt aataaaagat tctgaagcag tcgcctggta ttgcgaaaac gtcaactgcc
     1321 ctgctcaact caaccgcact atcgaaaact acgcctcgcg tactgcaatg gatatcgccg
     1381 gacttggaga gaaaattgtc gttcagctta cagaagcagg tcttttaaaa tctattgctg
     1441 atttatatat attgcgaaaa gatgagcttt tgaaactcga taaatttggc gaattgaaag
     1501 ccagcaacct gttaaatgcc attgaagcat ctaaatcgca atccttgcaa cgccttatca
     1561 tcggacttgg tattcatggc attggcgaag ttagcgccgg cgatttggca aggcacttca
     1621 aaagcctgga cgccttggct caagccagcc ctgatgacct gcaaagcatc ccaggcatcg
     1681 gcccaaacac tgctgaatcg atagttcatt ggttcagtca aacgcaaaat caagccacac
     1741 tccaacgcct taaaagctat ggggtctggc cagtcattca agagcaagcc aaaactgaaa
     1801 gccccaaacc tttggaaggt cttacctttg tcattactgg aaccctaccc aacatgagcc
     1861 gtgatgaagc caaacaactc attgaatcca acggtggcaa aacaaccggg tcggtgagca
     1921 agaacacaag ttatttgctc ttaggcgaaa atcctggttc aaaattagat aaagcacttg
     1981 aactcggcat cccaaccatt agcgaagaag atttgaataa tattcttaat atttgatatc
     2041 cataaatctt tccacttaac ataaaaaccg ccatctaatt catcattgat ggcggtcttt
     2101 tttgtggaga tggcgggagt cgaacccgcg tccgaaagat tagactctcg aatc
//
LOCUS       DAOSFC010000239         2117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_51971_length_2117_cov_5.714355, whole genome shotgun sequence.
ACCESSION   DAOSFC010000239 DAOSFC010000000
VERSION     DAOSFC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51971_length_2117_cov_5.714355"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PK791_05985"
     CDS             complement(<1..525)
                     /locus_tag="PK791_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOH92896.1"
                     /translation="MFRTRFAPSPTGYMHIGNLRTALYAYLIAKKAKGVFILRIEDTD
                     QKRNVSDALDAIYNGLALAGITYDEGPDKDGGHGPYVQSQRKAIYGQYAHQLIDQGDA
                     YYCFCQKEDRPEPGDERDEERRDPCRNLSPAEVQDFFDQGIQPVVRQRIPHEGTSSFF
                     DHVYGEISIENKQLD"
     gene            complement(697..1095)
                     /locus_tag="PK791_05990"
     CDS             complement(697..1095)
                     /locus_tag="PK791_05990"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOH92897.1"
                     /translation="MKSELSLEMPESSEMYLETILLLERNNPVVRQVDIARALGFKRP
                     TVHVAVKKLAEQKLVHIDEGSKIALTPQGRQIAEAVFMRHKTFTRFLMHLGVDEQTAE
                     MDACRIEHYVSEQTFQRIKDYCEKFLNGDC"
     gene            1185..1403
                     /locus_tag="PK791_05995"
     CDS             1185..1403
                     /locus_tag="PK791_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOH92898.1"
                     /translation="MVPLCYMQNKKRCRVRRARGRSRERMEALGFVPGEELDVISSCD
                     GNVIVLLRDARYALDKEQAKDIYVEEAV"
     gene            1400..1630
                     /locus_tag="PK791_06000"
     CDS             1400..1630
                     /locus_tag="PK791_06000"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOH92899.1"
                     /translation="MKTLFDMQPGQKAVVKKVHGEGNLRQRILDMGILRGTELEMIKK
                     APLGDPIEFKVKGYDLSLRQSEASLVEVEVRK"
     gene            1627..>2117
                     /locus_tag="PK791_06005"
     CDS             1627..>2117
                     /locus_tag="PK791_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOH92900.1"
                     /translation="MTKQALTIALAGNPNSGKTTLFNQLTGANQYIGNWPGVTVEKKE
                     GKLKGHPQVTIQDLPGIYSLSANSLEERIASEQLTQHSPDAIINIVDGTNLERNLYLT
                     MQILEMGIPTVVAINMADMLSRQGAKLDLDKLSKRLGCPVVGISALKGTHINELIAEA
                     LKVA"
BASE COUNT          538 a          467 c          563 g          549 t
ORIGIN      
        1 atcaagctgt ttgttttcga tactaatttc gccgtaaaca tggtcaaaga agctggatgt
       61 gccctcatgc ggaatgcgct gacgcacaac gggttgaatg ccttggtcaa aaaagtcctg
      121 gacttcagca gggctcaggt tacggcaagg gtcgcggcgt tcttcgtcgc gctcgtcgcc
      181 gggttcaggg cggtcttctt tttggcagaa gcagtagtaa gcgtcgcctt ggtcgatgag
      241 ttgatgggcg tattgtccat atatcgcttt gcgctgactt tgcacatagg ggccgtgtcc
      301 gccatcttta tcggggcctt cgtcgtaggt gatgccggca agggcgagtc cgttgtaaat
      361 ggcatcaagc gcgtcggata cgttgcgttt ttgatcggtg tcttcgatac gcaagatgaa
      421 cacgcccttt gcttttttgg caatcagata ggcataaagg gcggtacgca ggttgccgat
      481 gtgcatatag ccggtgggcg atggggcaaa acgggttcta aacataatca cttccattgt
      541 ttttgtaatc ctatgaatac taccataaag cgactatttc ggcgcgggat tttggggatt
      601 agtcagggta attaaatgcc ttgcttgcga ccagccatgc tgatccattt ggtaccattt
      661 tattctccga aagtgtttga acaggatgat agtctattaa cagtcaccgt tcaaaaattt
      721 ttcgcagtag tccttaatgc gctggaaggt ttgctcgctc acataatgtt caatgcggca
      781 ggcgtccatt tcggcggttt gctcatccac acccaggtgc atgagaaagc gcgtaaaggt
      841 cttatggcgc atgaaaacag cttcggcgat ttgccgtccc tggggggtca gcgcgatttt
      901 gctgccttcg tcgatgtgca cgagcttttg ttcagccagt tttttgacgg ccacatgcac
      961 ggtggggcgt ttaaagccga gcgcgcgcgc gatgtctacc tgacgcacga cagggttatt
     1021 gcgttccagc aggagaattg tttcaaggta catttcgctt gattcgggca tctctaatga
     1081 aagttcactt ttcatatctc aattatacca aaaacccttg acagaaaagt tagttgggtc
     1141 taacatataa tgagttagtt gatgctaaca actaaaggag taacatggta ccactatgct
     1201 atatgcagaa taaaaaacga tgtcgtgtca ggcgcgcgcg tggcagatcc cgcgaacgca
     1261 tggaagctct tggctttgtg cccggcgaag agctggatgt gatttcttct tgcgacggaa
     1321 atgttatcgt gcttttgcgc gatgcgcgct acgccttgga taaagaacaa gcaaaagaca
     1381 tttatgtcga ggaggcagtt tgaaaacatt atttgatatg caacccggcc aaaaggctgt
     1441 ggtcaaaaaa gtacatggcg aaggaaactt gcgccaacgc attttagata tgggcatttt
     1501 acgtgggact gaactggaaa tgattaaaaa agccccatta ggtgacccga ttgagttcaa
     1561 ggtgaaaggt tatgaccttt cattaagaca gtcagaggca tcattagtag aagtggaggt
     1621 gcgtaaatga cgaagcaagc cttaacaatc gccctggcgg gaaacccaaa ctcaggtaaa
     1681 acgacactgt ttaatcagtt gactggcgca aaccagtata tcggtaactg gccaggtgtg
     1741 acggtcgaga aaaaggaagg caagctcaaa gggcatccgc aggtcactat tcaagacctg
     1801 cccggtatct attccctttc ggcaaactcg cttgaagaac gcattgccag cgaacaactg
     1861 acccaacatt cgcccgatgc cattatcaac attgtggatg gcaccaacct ggaacgcaac
     1921 ctgtatttga cgatgcaaat acttgaaatg ggcattccga cggttgtcgc tatcaacatg
     1981 gcggatatgc tcagcagaca aggcgcgaaa ctggatttag acaaactctc taagcgcctg
     2041 ggctgtcctg tggtgggcat ttcggcgtta aagggcacgc atattaatga gcttatcgct
     2101 gaggctttga aagttgc
//
LOCUS       DAOSFC010000240         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_52006_length_2116_cov_6.621058, whole genome shotgun sequence.
ACCESSION   DAOSFC010000240 DAOSFC010000000
VERSION     DAOSFC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52006_length_2116_cov_6.621058"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="rpsD"
                     /locus_tag="PK791_06010"
     CDS             <1..633
                     /gene="rpsD"
                     /locus_tag="PK791_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOH92901.1"
                     /translation="RYTEAVCRLCRREGEKLFLKGERCFTPKCSFERRSYPPGMHGKS
                     LAFRPGRESDYAKQLRAKQKARRIYGIYERQFRRFFEQAVKMHGQPGPNLLTILETRL
                     SNVVYRLGFAASRPQARIMVTHGHFLLNGRRADIPSMEVKVDDVITVAETSKSTPFFK
                     NLREEAEGRNVPTWLARDLNSLSGRMLRNPERAEIDGNLNEQLIVEYYSR"
     gene            695..1654
                     /locus_tag="PK791_06015"
     CDS             695..1654
                     /locus_tag="PK791_06015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOH92902.1"
                     /translation="MEMVTPKVERTEESRNYGKFTISPLERGYGVTLGNALRRVLLSS
                     LDGAAVTAIRLTDVMHEFSTIPGVREDVIQVMLQLKLLRFKLHNVETAQLHLEVKGKG
                     TITAADIVCPPEVEIINPDLYLFSMNEDGHEVDIDLTVEAGRGYLPAGDRAERLPIGV
                     LPVDAIFSPIQRVNWSVSSARVGRSTDFDRLTLEIWTDGTISPERALIDASRILMQHL
                     SYISGTTEDMLIKPEPVVETEQLPQVKEINEAPIETLDLSVRVFNSLKRTGVMTVNDI
                     LELLEKGDSAMLSIRNFGEKSLQELKAKMAEKGYIENTEVEND"
     gene            1654..2037
                     /gene="rplQ"
                     /locus_tag="PK791_06020"
     CDS             1654..2037
                     /gene="rplQ"
                     /locus_tag="PK791_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOH92903.1"
                     /translation="MRHRVKGSRLNMRVGHRNSQRRTQVRQLFEYERIQTTVAKAKSI
                     QPDAERLITIAKNAIKGDDIAQVNGRRRVAAYLGNNSTEIVKKLFDDIAPRFTNRPGG
                     YTRLLKTGPRQGDNAEMALLELVSE"
BASE COUNT          578 a          515 c          514 g          509 t
ORIGIN      
        1 agatatacag aagcagtttg tcgattatgt cgacgcgaag gcgaaaagct gttccttaaa
       61 ggcgaacgct gctttacgcc caaatgctca tttgaacgac gcagttatcc ccccggaatg
      121 catggaaaat cgctggcatt ccgcccgggt cgtgagtctg actatgctaa gcagttacgc
      181 gccaaacaaa aagcgcgccg catctatggc atttacgaac gccagttccg ccgcttcttt
      241 gaacaagcgg taaagatgca cggacaacct ggtcctaacc tgcttactat tttggaaacc
      301 cgtctcagca atgtggttta tcgtctcggt tttgcagcca gccgtccaca ggcacgcatc
      361 atggttaccc acgggcactt tttgctcaac ggtcgccgtg cagacattcc atcaatggaa
      421 gtcaaagtgg atgatgttat cactgttgcc gaaacgtcaa aaagcacacc tttcttcaag
      481 aatcttcgcg aagaagctga aggtcgcaac gttcccacct ggttggcgcg cgatttgaac
      541 agtctcagtg gtcgcatgtt acgcaaccca gagcgcgcgg agattgacgg caacttgaac
      601 gaacagctga tcgttgaata ctactctaga taagagtgat tagtcagtct ttcttacccg
      661 ataggctggt taggggtaaa ccgtgacagg aattatggaa atggtaacac caaaagtaga
      721 acgcactgaa gagtcgcgta actacggaaa gtttacgata agcccgctgg agcgtggcta
      781 cggcgtcacc ctcggcaacg ccctccgacg cgtgttgctg tcgtcgcttg acggcgcggc
      841 agtcaccgcg attcggctta ctgatgttat gcacgagttt agcaccattc ctggtgtccg
      901 cgaggacgtt atccaggtga tgttgcagct gaaactcctg cgttttaaac ttcacaatgt
      961 cgagaccgcg cagctacatc tggaagtaaa aggtaaaggc accattacgg ctgctgatat
     1021 cgtctgtccg cctgaggttg aaatcattaa tcctgatctt tacctcttta gcatgaacga
     1081 agatggtcat gaagttgata ttgacttgac cgttgaagcc ggacgtggct atttgcccgc
     1141 tggcgaccgc gctgaaagat tgcccattgg cgttttgccc gtggatgcga tcttctcgcc
     1201 catccaacgt gttaactggt cagtttcaag cgcccgagtt ggcagaagca ctgactttga
     1261 ccgtcttact ttagaaattt ggacagatgg taccatttcg ccggaacgtg ctttgattga
     1321 tgcatcgcgc attctgatgc aacacctaag ctacattagc ggaactaccg aagatatgct
     1381 catcaaaccc gaacccgttg tggaaactga gcaacttccc caggtaaaag agatcaacga
     1441 ggctccgatt gagactttgg atctctctgt gcgtgttttt aactcactca aacgcacagg
     1501 tgtaatgacc gttaatgaca tcttggaact tctagaaaaa ggcgatagcg caatgctctc
     1561 aattcgcaat tttggcgaaa agagtctgca agaactaaaa gccaaaatgg ccgaaaaagg
     1621 atatattgaa aatacagaag tggagaacga ctaatgcggc accgagttaa aggatctcgt
     1681 ctgaatatgc gtgttggtca tcgcaactca cagcgacgca cgcaggttag acaactattt
     1741 gaatacgaaa gaatccaaac caccgtggct aaggcaaaat ctattcagcc cgacgctgaa
     1801 agacttatta ccatagccaa aaacgctatc aaaggtgatg acatcgccca ggttaatggc
     1861 cgacgccgtg tagctgccta tttgggcaac aacagcactg aaatcgtcaa aaaactgttt
     1921 gatgacatcg caccgcgttt tacaaatcga ccaggtggtt atacacgttt gctcaaaacc
     1981 ggaccgcgcc agggcgataa tgctgaaatg gcgctcttag agttggtttc tgagtaaatt
     2041 gaagaattat taagcctttc cgaaagttga agggcatggc acattacaaa cttattctcg
     2101 catacgaagg cactgc
//
LOCUS       DAOSFC010000241         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_52258_length_2109_cov_6.211295, whole genome shotgun sequence.
ACCESSION   DAOSFC010000241 DAOSFC010000000
VERSION     DAOSFC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52258_length_2109_cov_6.211295"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PK791_06025"
     CDS             complement(<1..468)
                     /locus_tag="PK791_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92904.1"
                     /translation="MRHSPKYLLLLLSMVLILATFGFSGVSARAVAVHHPDGYTNQSW
                     YLICEMDETGYVSVHSQMQVQLAAPLTNFSDEMIAEALARPNRNDQPVIVRLTDASGK
                     VVFQKTERVSGLIRSEFGGENPDDPIQGHFVERDKTVFVVRVPVIKGTTMSLKK"
     gene            complement(597..1685)
                     /gene="serC"
                     /locus_tag="PK791_06030"
     CDS             complement(597..1685)
                     /gene="serC"
                     /locus_tag="PK791_06030"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOH92905.1"
                     /translation="MKYDRIYNFSAGPSMLPLPVLEEVHEELFNYRGSGQSVMEMSHR
                     SKVFQGIHDEAKADLRALMGIPENYKILFIQGGATLQFSMIPMNLKKNGVFDYVVSGS
                     WSNGAAKEAARFGKVNVVANSKDRNYAYVPDVSDLPISEDADYLYICENETIYGTTYQ
                     ELPNTKGKILISDQSSMFLSKPVNVSDYGIIYGGVQKNVGPAGMAIVIIREDLIREDI
                     DESVPVYLRYHVQDKGDSMYNTPNCWAIYFCGKVFKYLQSIGGLEAVHKNNVEKAKPL
                     YDFLDQSKMFKGTVDKKDRSLMNVPFVTGDEELDAKVVAATKAAGFENLKGHRTVGGL
                     RASIYNAMPKEGVVALVEFLKDFEAKHG"
BASE COUNT          556 a          502 c          476 g          575 t
ORIGIN      
        1 ttttttcagg ctcatggttg tgcctttgat aactggcaca cgcaccacaa aaacagtctt
       61 atcgcgttca acgaagtgtc cctgaatagg atcatccgga ttttcaccgc caaattcact
      121 tcgaatcaag ccagatacac gttcagtctt ctgaaaaacg actttaccgc tcgcatcagt
      181 cagtcgcacg attaccggtt ggtcattccg attcggtcgc gccaaagctt cagcaatcat
      241 ttcatcagaa aaattagtga gcggtgccgc gagttgcacc tgcatttggg agtgaaccga
      301 gacatatccc gtttcatcca tttcacaaat caaataccag gattgattgg tatatccatc
      361 tggatggtgg acggcgacag cacgcgcgct gactcctgaa aaaccgaacg tggccagtat
      421 caagaccatt gacagaagca gtaacaggta cttgggggaa tgacgcattt ttacacctcg
      481 tttatttaat tttcctgcgc cattttatta cagatgtcta tttaaaaccg ccctttttac
      541 aaaaaaaggc tgcccgtgtt tttggggcag ccttcacgct tagaaacgaa ataattttag
      601 ccgtgtttgg cttcaaagtc cttcaggaat tcgaccagcg ccacaacgcc ttccttgggc
      661 atggcgttat agattgacgc gcgcagacca ccaacagtgc ggtgaccctt gaggttttca
      721 aagccggcag ccttggtggc ggcgacaact ttggcgtcaa gttcttcatc accagtaacg
      781 aaaggcacgt tcatcaagct gcggtctttc ttatccacag tacccttgaa catcttgctt
      841 tgatccaaga agtcatacag gggtttggct ttctcaacgt tatttttgtg aacagcttcc
      901 agaccgccga tgctctgcag atacttgaag actttgccac agaagtaaat tgcccagcag
      961 ttgggggtgt tgtacatgga atcgccctta tcctgaacat ggtagcgcaa gtaaaccggt
     1021 acgctctcgt cgatatcttc gcggatcagg tcttcgcgga tgatgacaat cgccatacca
     1081 gcggggccaa cgttcttttg cacaccacca taaatgatgc cataatcgga cacgtttaca
     1141 ggtttggaga ggaacatgga gctttggtca gaaatcaaaa tcttaccctt ggtgttgggc
     1201 agttcctgat aggtggtgcc atagatggtt tcattttcgc agatgtagag ataatcagca
     1261 tcttcgctaa tgggtaaatc agaaacatca ggcacgtagg cataattacg atctttgctg
     1321 ttggcaacaa cattcacttt accaaaacga gctgcttctt tggcagcgcc atttgaccaa
     1381 ctaccgctta caacgtaatc aaaaacgcca tttttcttga gattcatggg aatcatcgag
     1441 aattgcaggg ttgcgccacc ctgaatgaag agaatcttat aattctcggg aatgcccatc
     1501 aaggcgcgca aatcggcttt ggcttcgtca tgaatgccct ggaaaacttt gctgcgatgg
     1561 ctcatttcca tcacactttg gccggaacca cgatagttga ataactcttc gtggacttct
     1621 tccaagactg gcaagggtaa catgctggga ccggctgaga aattgtaaat tcgatcgtat
     1681 ttcattttta ttaatctcct ttatggttgc ttgtttatct tcaggcacac aaaaacaaaa
     1741 cctgcttatt tttgggcaag ttggcctgtt gaaatcatag tccgcaatgc cgattcggtc
     1801 aagtgaatca gacctaaatc ggtgatttta gcgttattaa aacgcctttt tagcggtcag
     1861 ctacgcctgt attaggttgt taaattatgc ctgtaaacgc caccacttaa aaactgtaaa
     1921 tcgcatttta cctgttcgcc gggctgcttt ataatcagac catggcaaaa ttagtccgtg
     1981 cggaaaagca aaactggttg ctccttgaaa aaataggcag tggcgacgcg ggtgaagtct
     2041 ggcgtgttga atccgaaaca agccagcaac gcgccgtact caaacgcccg gtgcagaatg
     2101 tatccggtg
//
LOCUS       DAOSFC010000242         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_52568_length_2101_cov_4.608993, whole genome shotgun sequence.
ACCESSION   DAOSFC010000242 DAOSFC010000000
VERSION     DAOSFC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52568_length_2101_cov_4.608993"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PK791_06035"
     CDS             complement(<1..865)
                     /locus_tag="PK791_06035"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOH92906.1"
                     /translation="MTGTDKKADFYIGDIPIHGRLILAPMDGLSDTVFRRITRQLGSA
                     YSVSEFINTLDFMYSKVYQRKRLFFHEDERPFVAQILDNDPVRMAENAAYIQERFKPD
                     MIDVNLGCPARTVSGRGAGCALMKSPELVKQIFEQVCKVVTIPVTAKIRLGWDDNSMN
                     YMEIASIVVERGAKAVAMHARTKKMAFGKTVRWEHIATLKKAMPVPVIGNGEVRSVAD
                     AKRMFEETGCDAVMIGRAAKANPWIFSWRDREQVAVEEVFYLARYQLRQMLEVNPENA
                     IMPFRKYLKAYL"
     gene            complement(866..>2101)
                     /locus_tag="PK791_06040"
     CDS             complement(866..>2101)
                     /locus_tag="PK791_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HOH92907.1"
                     /translation="GYKTYWLCFSDSVETEDELYFEDVDDDEVAELENEWLKITLDRF
                     DGSFSILDKRNGAVYENQGVLASVGDRGDEYNFTPTPDDSLYLIEVKNVAYVPRAFDH
                     VIGVIFDMVLPEGLDYETMDRSEETIVSRGMMLISLDPGQACVDISVFFENNACDHRL
                     AMHFDVDFPIEKVLYDGHFDIVERPLDLPEFDESWREHPRPEQPQGAFVDVLGHKQGL
                     MIANQGLPEVAVKRYEDSDSSSIAITLLRAVSELSRSDLFNRFGHAGPEYTTPAAQML
                     GENLEYNLRIIPHDGNSSVARPLAYAFQSQPHTVVTPLQAGDLPSEAYMIDVNHPDFH
                     VTAIKEAEDKQGYIVRGYNQSGEPILVNLRSHMLAKRAAKVRLDEAFLEELTLSADGA
                     VNFEAQPKEIVTVYFGLKA"
BASE COUNT          530 a          584 c          481 g          506 t
ORIGIN      
        1 ccaaataagc tttgagatat ttcctgaagg gcataatggc gttttccgga tttacttcca
       61 gcatttgtcg taattgatag cgcgccaaat aaaacacttc ttccacagcg acctgctcac
      121 ggtcacgcca tgaaaatatc catgggttcg ccttggccgc gcggccaatc atcaccgcgt
      181 cacaaccggt ttcttcaaac atacgtttgg cgtcggcaac actgcgcact tcgccattgc
      241 caatgaccgg taccggcatg gctttcttta gcgtggcgat atgctcccag cgcaccgttt
      301 tgccaaaggc catctttttt gtgcgcgcgt gcatcgccac cgctttcgcg ccccgctcta
      361 ccacgatgga cgcaatctcc atgtaattca tcgaattatc atcccaaccc aggcggattt
      421 tcgcggtcac cgggatcgta accaccttgc aaacctgttc aaagatctgc ttgacgagtt
      481 cggggctttt catcagcgcg caacccgctc ccctgccgga aaccgtccgc gccggacagc
      541 ccagattgac gtcaatcatg tctggcttga agcgttcctg gatataagcc gcgttttctg
      601 ccatgcgcac agggtcgtta tctaaaattt gagctacaaa ggggcgctca tcttcgtgga
      661 agaacaaacg cttgcgctga tagacctttg aatacatgaa atcgagcgtg ttgatgaatt
      721 cggagaccga ataagccgaa ccgagttgac gcgtgatgcg ccgaaaaacc gtatcagaaa
      781 gcccatccat gggtgccaaa atcaggcgtc catggatggg aatatcgcca atatagaagt
      841 ctgctttttt atccgttccc gtcatttagg ctttcaaccc aaagtaaacc gtcacaattt
      901 cttttggctg ggcttcaaaa ttcaccgcgc catccgcgct aagagttaat tcttccagaa
      961 aagcctcatc caaacgtacc ttagcagccc gttttgccag catatggctg cgcaggttta
     1021 caagaatggg ctcaccactt tggttataac cccgcacgat atagccctgc ttgtcttccg
     1081 cttccttgat ggcagtaacg tgaaaatcgg gatggttcac gtcaatcatg taagcttcag
     1141 atggcaaatc gccagcctgt aatggcgtta cgacggtatg tggctggctt tggaaagcgt
     1201 aagccaaggg gcgcgccacg gagctgtttc cgtcatgggg aataatacgc aggttatatt
     1261 ccagattctc acccagcatt tgcgcggcgg gggttgtgta ttccgggccg gcatgcccaa
     1321 agcggttgaa caaatcagac cgcgaaagct cgctgaccgc gcgcaacaaa gtgatggcaa
     1381 tgctcgaaga gtctgaatct tcatagcgtt tcaccgcgac ttccggcaag ccctggttgg
     1441 caatcatcaa gccctgtttg tgaccaagca catccacaaa ggcgccctgc ggttgttcgg
     1501 gccgggggtg ttcgcgccag ctttcgtcaa attcaggcaa atcaagcggg cgttcaacga
     1561 tatcaaaatg gccatcataa agcacctttt cgatggggaa atcgacgtca aaatgcatcg
     1621 caaggcgatg gtcacacgcg ttattttcaa aaaacacgga gatatccaca caagcctgac
     1681 ctgggtcaag actaatcagc atcatgcccc ggctaacgat tgtttcttca gaacgatcca
     1741 tcgtttcata atccaaacct tccggcaaga ccatatcaaa aatgacgcca atcacatggt
     1801 cgaaggcccg cgggacgtat gcaacgttct taacctcaat caggtatagt gaatcatccg
     1861 gggtgggtgt aaagttatac tcatcgcctc tgtcacccac agatgccaaa acaccctgat
     1921 tctcataaac cgcgccattg cgtttgtcca aaatgctgaa actgccatca aagcggtcca
     1981 aagtaatctt cagccattcg ttctccagct ccgcaacctc atcatcgtcc acgtcttcaa
     2041 aataaagttc atcttccgtt tcgacagagt cgctaaagca cagccaataa gttttataac
     2101 c
//
LOCUS       DAOSFC010000243         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_52767_length_2095_cov_4.512745, whole genome shotgun sequence.
ACCESSION   DAOSFC010000243 DAOSFC010000000
VERSION     DAOSFC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52767_length_2095_cov_4.512745"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PK791_06045"
     CDS             complement(<1..314)
                     /locus_tag="PK791_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92908.1"
                     /translation="MTKATALINARIYVDWHNPPVEALLLDHGLVRLAGTNQEVLSFL
                     PNSVKRLDMQGMTIWPGLCDAHLHLEQLSNQLASINCEGLSKAEILELVRLKAQSLPN
                     GT"
     gene            complement(307..1179)
                     /locus_tag="PK791_06050"
     CDS             complement(307..1179)
                     /locus_tag="PK791_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOH92909.1"
                     /translation="MAKTTGLGKGLGALIPGWQDEAKSSDTLTADQVPLIPINQIRPN
                     PHQPRKTFNQESLEDLAASIREHGIIQPLIVISDGPNQYTLIAGERRLRASKIAGLKE
                     VPAIVRTATEQEQLEFAIIENVQREDLNPIERARAFQRLIDDFSLTHEEVAQRVGKSR
                     VTVTNALRLLNLPVGVQQAVLSGSISEGHARALLALNSPRAIENVMETVISLGLNVRQ
                     TELLVNKTLGRVSSKTKTAERSPELDSLENELRQHFHTKVTLVKNKNGGSITIFFYSD
                     EELNAITDRFGLYD"
     gene            complement(1172..1969)
                     /locus_tag="PK791_06055"
     CDS             complement(1172..1969)
                     /locus_tag="PK791_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH92910.1"
                     /translation="MARTYTVVNQKGGVGKTTTSINLSAKLGEYGLRVLIVDLDPQAN
                     ATASLGVERHKLNTGIYELLIGTGNVNETILHHPRYNISLIPSTVNLSGAEIELIDLP
                     DRDKRLYNVLTPISDRYDYIIIDCPPSLGLLTLNGLIAARDGVLIPVQCEYLALEGLG
                     QLTQTLHRIRQNLFPELTIRGVLLTMFDGRTNLSADVRAEVQKFFPEQVFESYIPRSV
                     RLAEAPSFGMPISVYAPDSSGAKAYDALAREILLSDGYDLKTEKKHG"
BASE COUNT          516 a          456 c          554 g          569 t
ORIGIN      
        1 catgtgccgt ttggtaggct ttgtgctttt agacgcacta attcgagaat ttcagctttt
       61 gataagcctt cgcagttgat acttgccagt tgatttgaaa gttgctctaa atgcaagtgc
      121 gcgtcacaca agccaggcca aatggtcatg ccttgcatgt ctagtctttt tacggaatta
      181 ggtaaaaagg aaagaacttc ttgatttgtc ccagcaagac gaaccaaacc atgatccaaa
      241 agtagcgctt cgactggcgg gttatgccaa tccacataga tacgtgcgtt gattaaagct
      301 gtagctttag tcatacaaac caaagcggtc ggtgatggcg tttagttctt catcagaata
      361 gaagaatatt gtaatggaac cgccattctt gtttttcaca agggtgacct tggtatggaa
      421 gtgctgacga agttcgtttt cgagcgaatc aagctctggg ctacgctcag cggtcttggt
      481 ttttgacgag acacggccaa gcgttttgtt gaccaataat tccgtttggc gcacattgag
      541 acccagcgaa atgaccgttt ccatcacatt ttcaatagcg cgcggactgt ttagggccag
      601 cagggcacgg gcatggcctt cgctgatgct tccgctcaaa acggcttgtt gaacacctac
      661 tggaaggttt agcaggcgca aggcgttggt tacggttacg cggctttttc caacgcgttg
      721 ggcaacttct tcgtgagtaa gcgaaaaatc gtctatcaag cgctggaatg cgcgagcgcg
      781 ctcaatcgga ttgaggtctt cgcgttgtac gttttcaata atggcaaatt caagttgttc
      841 ctgttcagtg gcagtacgta caatggcggg aacttctttt aaaccagcaa tttttgaagc
      901 acgcaaacgt ctttcaccag cgataagcgt atattggttt gggccatcac taataacgat
      961 gagtggctgg ataatgccat gctcgcgaat ggaggcagcc aggtcttcaa gactttcctg
     1021 gttaaaggtt ttgcggggct ggtgcggatt tgggcggatt tgattgatgg gaatcagggg
     1081 cacctggtcc gcagtcagcg tgtcgcttga ttttgcttcg tcttgccatc ctgggatgag
     1141 cgcgcctaaa cctttgccta gtccagttgt tttagccatg ttttttctcc gttttaagat
     1201 cataaccgtc tgataataat atttcacgtg ccagtgcgtc atatgctttt gcgccgctcg
     1261 aatctggcgc gtagaccgat atgggcatgc caaaagatgg ggcttcagca aggcggacgc
     1321 tgcgcggaat ataactttca aatacctgct cagggaagaa tttttgcact tccgcacgca
     1381 catcagcgct tagattggtg cgaccgtcaa acatggtgag caaaacgcca cgaatagtta
     1441 gttcaggaaa aagattttgg cggatgcgat gcagagtttg ggtaagctgc cccaagcctt
     1501 caagcgccaa atattcgcat tgaacgggta tcagcacgcc atcacgggcg gcaatcaaac
     1561 cgtttagggt tagcaaacca agggatggcg gacaatcgat aatgatgtaa tcatagcggt
     1621 cagatatcgg tgtgagcacg ttatataagc gtttatcgcg gtcgggcagg tcgatgagtt
     1681 caatttcagc gccagaaagg ttcactgtag aaggaataag cgagatatta tagcggggat
     1741 ggtggagaat cgtctcgttg acattcccag tgccgatgag cagttcatat ataccggtat
     1801 tgagtttgtg acgttccaca cccaaacttg cggtggcgtt ggcctgggga tccaggtcaa
     1861 caatgagcac gcgcaagcca tattcgccaa gtttggcact aagattgata ctggtggtgg
     1921 ttttgcccac gccacctttt tggttgacga cagtataagt tctagccata aacagcctgt
     1981 tctaaagcga gatgtgtcgc tttttgaatt tcttcctgag taggtatgct attaaaatgt
     2041 tcacgggtaa cggattggct ggcaagttca accgcaaaac gcgcggcttg ccagg
//
LOCUS       DAOSFC010000244         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_53117_length_2086_cov_6.612014, whole genome shotgun sequence.
ACCESSION   DAOSFC010000244 DAOSFC010000000
VERSION     DAOSFC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53117_length_2086_cov_6.612014"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PK791_06060"
     CDS             <1..799
                     /locus_tag="PK791_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HOH92911.1"
                     /translation="IGKHAFWIILAVVVVVILLRAAIYVVPAGNVGVITRWGAANRTS
                     LAGIHVKIPFAENVIMMSTRTQKDESPATAMSNNLQVVSSQIAVNYRLDGNKALQVFQ
                     EIGPNYAEIVVAPAIQNAFKAATARFTAEELITKRGEVRQMAEDELIAQIEPYHIIVE
                     NFNIINMDFSAEYQNAIEAKQVAQQQVETSKQKLAQAEIDAQTVIAQAQGQADAQKAL
                     KDTGALTPEYLQYLFLTKWNGILPQVMTSGSGMMLDITQFMQGPEKP"
     gene            complement(960..1634)
                     /locus_tag="PK791_06065"
     CDS             complement(960..1634)
                     /locus_tag="PK791_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92912.1"
                     /translation="MSSTHKRILNLIVILGLLLVMLPQQGSIAQADVSNPVSPNQPDD
                     SEEIFRQPYAGPERELDNTIQAGDKILNIAGWHFYPYFEQCEGRFRGESFGKIGPKGT
                     LSASCFITYPVLIPYGSTFEWVFFNYYDADPDRDLKFQIIKNSYLGDEAGTIIVEKFS
                     SGADGYGYTPAIRVNHKMGMYDTYQIIVTFPAGASNTLLLSSVNIQYSEPPPALFGLG
                     FPAIQK"
     gene            1858..>2086
                     /locus_tag="PK791_06070"
     CDS             1858..>2086
                     /locus_tag="PK791_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOH92913.1"
                     /translation="MTLTTPVLDILLRLGLALILGGVVGFERERDSQPAGLRTHMILV
                     LGACLAAIISEEMGARFGSDPTRLAAQVISGI"
BASE COUNT          577 a          511 c          443 g          555 t
ORIGIN      
        1 aataggtaaa cacgcctttt ggattatcct tgctgtagtc gttgttgtta tattactgcg
       61 cgcagccatt tatgttgttc ccgccggtaa tgtgggtgtt attacccgct ggggcgctgc
      121 aaatcgcaca tccctggcag gcattcatgt caaaatcccc tttgcggaaa atgtgatcat
      181 gatgagcacc cgtactcaaa aggacgaatc tcccgccacg gcaatgtcca acaacttgca
      241 ggtggtttca tcgcaaatcg cggtcaacta tcgcctggat ggcaacaaag ccttgcaggt
      301 ttttcaggaa atcggcccca actacgctga aattgtcgtc gcgccggcga ttcaaaacgc
      361 cttcaaagcc gccacagccc gctttaccgc ggaagaactc atcaccaaac gcggtgaggt
      421 tcgccaaatg gcggaagatg aactcatcgc ccagatagaa ccctaccata ttattgttga
      481 gaacttcaac atcatcaata tggatttttc cgcggaatat caaaacgcga ttgaggccaa
      541 acaggttgct caacaacagg tagaaacatc caagcaaaag ctcgcccagg ccgaaattga
      601 cgcccaaacc gtcatcgccc aggcgcaggg tcaggctgat gcccaaaaag cgctcaaaga
      661 caccggcgcg cttacgccag aatatctgca atacttgttc ctgaccaaat ggaatggcat
      721 cctgccccag gtcatgacca gtggttcagg catgatgctg gatatcacgc aattcatgca
      781 gggccccgaa aaaccataag atttttttta gactgttccc ataagagcca ggtgtcctaa
      841 gtaacgcgcc tggctctttg ctattcccag gcaaactggc attagataaa atcagagcgt
      901 gccttttgac acgctctgta ttgtctaaat aatggtcact aatctctata gttcaaaccc
      961 tatttttgaa tggctggaaa tcccaggcca aaaagtgcgg ggggcggttc cgaatattgg
     1021 atgttgacac tacttaataa aagggtatta ctcgctcccg caggaaaagt tacgattatc
     1081 tgataggtgt catacatacc catcttatga tttaccctaa tagctggtgt ataaccatat
     1141 ccgtcggcac cagatgaaaa tttttccact attatagtac cagcttcatc acctaaatag
     1201 gaatttttaa ttatttggaa tttcaaatct ctatcagggt cagcgtcata gtaattaaag
     1261 aaaacccatt caaatgtaga tccataaggt ataagcacag gataggtaat gaagcacgaa
     1321 gcagagagag tccctttggg gcctattttg ccaaaactct cacccctaaa acgaccttcg
     1381 cattgttcaa aatatggata aaaatgccag ccagcaatat tcagaatttt atcaccagct
     1441 tgaatggtgt tatccaactc tctttctggc cctgcatagg gttgcctaaa gatttcttct
     1501 gaatcgtctg gttggtttgg cgaaacagga ttgctgacat cagcctgagc aatactgcct
     1561 tgttggggca acattacaag cagaagccct aaaattacaa taaggtttag aatgcgtttg
     1621 tgtgtgcttg acatgatacg tcctccttgt tgataattta ataaaatttt actctacgat
     1681 gctatttttg ggtgtttttt tcctctgatt tttttgcttt tcacttgaat cttaatttca
     1741 cactcgcatt ccatcataat agaaacagct gagcaaacgc acactccatt tcgcgccttt
     1801 ttaaaaacat gataaaatta gcatcaccta aacaaaagaa agacgcacaa accaacaatg
     1861 accttaacca cacctgttct cgacatttta ctccgcctgg ggctcgccct gatactcggc
     1921 ggtgtggttg gctttgaacg cgaacgcgac agccagccgg caggtttgcg cacgcatatg
     1981 attttggtgc ttggcgcttg tttagcggcg attatctccg aagaaatggg cgcgcgattt
     2041 ggttcagacc ccacccgcct tgccgcgcag gtcatctcgg gtatcg
//
LOCUS       DAOSFC010000245         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_53185_length_2084_cov_7.162642, whole genome shotgun sequence.
ACCESSION   DAOSFC010000245 DAOSFC010000000
VERSION     DAOSFC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53185_length_2084_cov_7.162642"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PK791_06075"
     CDS             complement(<1..521)
                     /locus_tag="PK791_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOH92914.1"
                     /translation="MLDLNIGTIIWEIVNFLIITVVLYFLVFKPMTKRAEARAIEKAE
                     NKAALERDLEEAAKRLREIDDRLSNLDEEVQVITDEAYSNSQILQRELLDATRQEADL
                     ILLNAVQEARKEQAIDIKKNQIDLVDTVISISNKTLRSVTPKSVHDALLEELHQTVWN
                     LGKTDMRTVQNIR"
     gene            complement(525..803)
                     /gene="atpE"
                     /locus_tag="PK791_06080"
     CDS             complement(525..803)
                     /gene="atpE"
                     /locus_tag="PK791_06080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOH92915.1"
                     /translation="MAHLSEEAIITIVTIIVSGAAIVIGTMIPTWVEGKSAVKAIEGM
                     ARQPEAAGQLRTAMLIALALLETASIYVLLIVLVLLFANPLITRFFGG"
     gene            complement(790..1515)
                     /gene="atpB"
                     /locus_tag="PK791_06085"
     CDS             complement(790..1515)
                     /gene="atpB"
                     /locus_tag="PK791_06085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOH92916.1"
                     /translation="MIEHKTLILLGMPISDTVVYTWITMGIIIAIVLLLKLLKPNLLE
                     HILELVIGIVDDAMDVDDLHPYIPVLGSLMIFVLVSNSISIVPGMNSPTSDINTTISL
                     ALIIVFSVHVYGARKKGLWKYLKEFASPIFMLPIELIGQLSRTLSLSLRLFGNVLSGD
                     LIVAIVFSLVPMVLPVAAVAITGISGVLQAYVITTLAALFISSAVEINEEDQKIKEEK
                     AAKRREKRKLRKKNRKEIANGTS"
     gene            complement(1508..1870)
                     /locus_tag="PK791_06090"
     CDS             complement(1508..1870)
                     /locus_tag="PK791_06090"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOH92917.1"
                     /translation="MLSPAVISILSYALWVVLGFASAVMTFSMLTRQISKIDPHDPNA
                     KKAVAPITIGRVARLLVIAVLIYLALKWNFRYAITFVVALTVATWGLAIYYHLRAKKA
                     EAVPSPAQTAKEEAEKND"
     gene            complement(1839..>2084)
                     /locus_tag="PK791_06095"
     CDS             complement(1839..>2084)
                     /locus_tag="PK791_06095"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOH92918.1"
                     /translation="ALPIVIGPVIGFFIDRSKGTGVRWTLILLGVGLFAAVTAVVRYV
                     SHEMHIMHRDEEAKRKEAEAKRREEERRAITRRYFDS"
BASE COUNT          562 a          534 c          458 g          530 t
ORIGIN      
        1 tcacggatat tttgcaccgt gcgcatatcg gttttaccca aattccaaac agtttgatgc
       61 aactcttcaa gcaaggcgtc atgcactgat tttggggtaa cagaacgcaa ggttttgttt
      121 gaaatgctta ttaccgtgtc aaccagatca atctgattct ttttgatgtc aatagcctgt
      181 tccttgcggg cttcctgcac cgcattgagc aaaatgagat ctgcctcctg acgggtggca
      241 tccaaaagct ctctttgcaa aatctgactg ttactgtaag cttcatccgt aataacttga
      301 acttcctcgt ccagattact aagccggtcg tcaatttcgc gcaatctttt agcagcttct
      361 tccaggtcgc gttcaagcgc ggctttgttt tctgctttct cgatggcgcg ggcttcggcg
      421 cgtttggtca tcggcttaaa gaccaggaaa tacagcacca cggtgataat cagaaagttg
      481 acaatctccc aaatgatggt gccaatgttc aggtcaagca tggtttagcc tccaaagaag
      541 cgcgtaatca gcgggttggc aaacaagagt accaaaacga tgagcagaac gtaaatcgac
      601 gcggtttcga gcagcgccaa agcgatgagc atcgcggtgc gtaattgccc agcggcttcg
      661 ggttgacgtg ccataccttc aatcgccttt accgcggact ttccttctac ccaggtggga
      721 atcatggtac caatcacgat tgccgcgcct gaaacgatga tggttacaat ggtaatgatt
      781 gcttcttcac taagatgtgc cattagcaat ctcctttctg tttttctttc taagttttct
      841 tttttcacgc cgtttggcgg ctttttcttc ttttattttt tggtcttctt cgttgatttc
      901 aaccgcggaa gagataaaca aggctgccag ggtggtaatg acataagcct gtaaaacacc
      961 cgaaataccc gtaatggcta ccgcggcaac aggcaaaacc ataggaacca gcgagaacac
     1021 aatcgccaca atcaaatcac cactcaaaac gttcccgaac aaacgcaggg aaagcgaaag
     1081 cgtgcgactc aattgcccaa tcaactcaat gggtaacata aaaatcgggc tggcgaattc
     1141 tttaagatat ttccaaagtc cttttttacg cgcgccataa acatgcaccg aaaagacaat
     1201 aatcagcgcc aaactaatcg tggtgttgat atcgctggtg ggcgagttca tgccaggcac
     1261 aatcgaaatg ctatttgaaa caagcacaaa aatcatcaaa ctgcctaaaa cagggatata
     1321 aggatgaagg tcgtccacat ccatagcgtc atcgacaata ccgatgacca gttccaggat
     1381 atgctccaaa agattaggct tcaataactt cagcaaaagc acaatcgcga tgatgattcc
     1441 catggtaatc caggtataaa ccaccgtatc gctgatgggc attcccagaa gtatcagcgt
     1501 tttatgctca atcattcttt tctgcttcct cctttgctgt ttgtgccggg cttgggacag
     1561 cttcagcttt tttggcacgc aaatgataat aaatcgccag tccccaggtc gccacggtca
     1621 aagccaccac gaaggttatc gcgtagcgga aattccattt taaggcgaga tagatcagaa
     1681 ccgcgataac caacaaacgt gccactcgcc ctatggtaat cggggcaact gccttcttcg
     1741 cgttggggtc gtgggggtct atcttgctta tctgcctggt aagcatgcta aaagtcatca
     1801 cagcactggc aaagcccaaa accacccaca aggcataact aagaatcgaa ataacggcgg
     1861 gtgatagcac gtcgctcctc ctctctgcgt ttagcttcag cctcttttcg tttggcttct
     1921 tcgtcgcgat gcatgatatg catctcgtgg ctcacataac gcaccactgc tgttaccgcg
     1981 gcaaacaaac ccacacccag taaaatcaaa gtccagcgca cgcccgtgcc cttgctgcgg
     2041 tcgataaaaa agccgataac cggaccgatg acaatcggca aggc
//
LOCUS       DAOSFC010000246         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_53692_length_2071_cov_5.017857, whole genome shotgun sequence.
ACCESSION   DAOSFC010000246 DAOSFC010000000
VERSION     DAOSFC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53692_length_2071_cov_5.017857"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PK791_06100"
     CDS             <1..701
                     /locus_tag="PK791_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92919.1"
                     /translation="IILEVQGIQSYITHYHILENVTFDVPKGSIVALLGRNGAGKTTT
                     LKSIMNLVDVREGQILFEGQQIQGLTPYEVAQRDIGYVPEHRAIFRDLSVLENLRIAE
                     RKKGDFDAKREMIFELFPDLKKFINLRGNELSGGQQQMLAIARALVPENRLLLIDEPS
                     EGLAPVIIEQLIEVIAQLAKQSTVILVDQNFLVTSKLADYYVIIEEGKSVERGVMAEL
                     INDHAKIQRYLGVA"
     gene            714..>2071
                     /locus_tag="PK791_06105"
     CDS             714..>2071
                     /locus_tag="PK791_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92920.1"
                     /translation="MKKKINITGILLIAAFIVFLVLAGRGMEPRDLLITTLRGFSVGS
                     VTFLVAAGFSLIFGLLDVLNLAQGTLYMIGAYIGWTVFVRPDTFIDITPLILFLMAGF
                     ALGFLWDYLADKITLDARKKKILGWVFVVLSVVLAVLILPRYPIAMWELDNYAQSPIS
                     FSYMADQGIRLPVSPAETKGIPAMVALYGSFLISAVLAFGLALIRYKENRQHSLHWKK
                     WRVFGILIILGTLIMAFNTPLTEWLMNMNTNLLFVIAAIIAVLSGIGLGALMETTMIQ
                     PLYSRPVYQLMLTLGLGTIGMQLVKAIWGMPEFVIPKPEMFRGSGPGCPATTVKDVFT
                     HHCSTIMILGGRVRVYDEIVLPIVGVIILVLVWLLLKKTRIGMIIRAGVQDSNMVEAL
                     GINVRRVFTLVFALGAGLAALGGVLSAPSYGLSVSMGEKFLLNALIALAIGGLTSYPG
                     AAL"
BASE COUNT          490 a          447 c          534 g          600 t
ORIGIN      
        1 aaatcattct ggaagtccag ggaattcaaa gctacatcac tcattaccat attctggaga
       61 atgtcacttt tgatgtccct aaagggtcca ttgtggcact tctgggacgc aatggagccg
      121 gaaaaacgac cacgcttaaa tctatcatga acctggttga tgtccgagag ggacaaatcc
      181 tctttgaggg ccaacagatt caaggactaa ccccatatga agttgcccaa cgcgatatcg
      241 gctatgtgcc cgaacatcgt gctattttta gagatttgag tgtattagaa aatctccgta
      301 ttgctgaacg taaaaagggc gactttgacg cgaagcgtga gatgatattt gagcttttcc
      361 cagatttgaa aaagttcatc aatcttcgtg gaaatgagct ttcgggtgga caacaacaga
      421 tgttggctat cgctcgcgcg ttggtgcctg aaaaccgact gcttttgatt gatgagccga
      481 gtgaagggct cgcaccggtc atcattgaac aactgattga agttatcgct caacttgcca
      541 agcaaagcac agtaatcctg gtggatcaaa actttttggt gaccagtaaa ctggcagact
      601 attacgtcat tatagaagaa ggcaaaagcg ttgagcgcgg tgtgatggca gaattaatca
      661 atgaccatgc caaaatacaa cgctatctgg gcgtcgccta aaaggagaaa cagatgaaaa
      721 agaaaattaa cattactggc attcttctga tagctgcttt tatcgttttt ttggtgctcg
      781 ctggtcgggg gatggagccc cgtgatttgc tgattactac cttgcgtggt ttctcggttg
      841 gctcagtaac attcctggtc gcagctggtt tttcgctcat ttttggtttg ctggatgtgc
      901 tcaacctggc acagggcacg ctatatatga ttggcgcgta tatcggctgg accgtatttg
      961 tgcgccccga tacttttatt gacatcaccc cgctgatcct ctttttgatg gcaggtttcg
     1021 cgcttggctt tctgtgggat tacctggctg ataaaattac gttggatgcc aggaaaaaga
     1081 agattttggg ctgggtcttt gtggtacttt cggtcgtact ggctgtttta attctgccgc
     1141 ggtatcccat cgcgatgtgg gaattagata attacgctca gagccccatt tctttttcct
     1201 atatggctga ccaaggcatt cgcttgccgg ttagcccagc agaaacgaag ggcatccccg
     1261 cgatggtcgc gctatatggc agctttttga taagcgcggt tttggcattt ggtttggcgt
     1321 taatccgcta caaggaaaac cgacagcaca gcttacactg gaaaaaatgg cgtgtctttg
     1381 gcatcttaat tatcttgggc accttaatca tggcctttaa tacccccttg acggaatggc
     1441 tgatgaatat gaacaccaac ttgttgtttg ttattgcagc aatcattgcg gttctaagtg
     1501 gaataggtct gggcgcgctg atggaaacca ccatgattca acctctttac agccggccgg
     1561 tctaccagct gatgctcacc ctgggacttg gcacaatcgg tatgcaactg gtgaaagcca
     1621 tttggggtat gccagaattt gtcattccca aaccggaaat gtttaggggt agtgggccag
     1681 gttgtccagc gacaacggtt aaagacgtgt tcacccatca ttgttcaact attatgattt
     1741 tgggtggcag agttcgggtt tatgatgaaa tcgtactgcc cattgtgggt gtgattatat
     1801 tggttttggt ctggctctta ctcaagaaaa cccgcattgg catgattatc cgtgctggtg
     1861 tgcaagacag taacatggtt gaagccctgg gcatcaatgt gagacgcgta tttaccttgg
     1921 tctttgcttt aggcgcaggc ctggcagctt tgggtggcgt gctttccgcc ccatcctatg
     1981 gcttgtcggt gtcgatgggt gaaaaattct tgctcaatgc gctgatcgct ttggcgattg
     2041 gtggtcttac cagttatccg ggcgcggcgc t
//
LOCUS       DAOSFC010000247         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_53801_length_2068_cov_7.471932, whole genome shotgun sequence.
ACCESSION   DAOSFC010000247 DAOSFC010000000
VERSION     DAOSFC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53801_length_2068_cov_7.471932"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PK791_06110"
     CDS             <1..718
                     /locus_tag="PK791_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH92921.1"
                     /translation="IWTVAMKRKEFRKEHQKKHPYPRFRTRRAILRNGIAFLASILLN
                     YKVEGQENLPEKGPLLIVNNHFHFLDTISPIHSTKYPLEFIGDHVMPNAPVALRVLPR
                     LWRTLEIQQGTANLEAIRAAEAILAQDGILAIMPEGHVHKPPLREALPGAAFLALRLG
                     VPILAIGTYSEDNWDIFGTIRNKKRKARVISKIGKVFGPLKIADGGNRPSRDDVNRAR
                     HEIMSQIAALLPPEVRGEFG"
     gene            812..1804
                     /locus_tag="PK791_06115"
     CDS             812..1804
                     /locus_tag="PK791_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOH92922.1"
                     /translation="MEYKDYYKTLGVSKTATDDEIKKAYRDLARKYHPDVNKDLKAEE
                     MFKEVNEAYQVLSDSEKRQKYDNLGSEWQQYQRAGGQPGGFDWGRWQQQPGQGGGYRT
                     MTPEEFQEIFGGMGMPGGMGSFSGMGGFSDFFTSLFGGGFSGGFSPGTETSYSQGGRS
                     YRTQAPARRDVEHETEITLEEAYRGTQRSLRFSDGRTINASIPAGVDTGSKVRLSGQA
                     DGADLYLLIKMLPHKVFTRKGNNLLLTIPVDFYTAALGGEVTVPTMEKPVKLNIPAGT
                     DSGKTFRLKGLGMPSLRKSDQKGDILAKVQIHLPKPLSQAEKSKLQALRDEYHS"
BASE COUNT          574 a          506 c          498 g          490 t
ORIGIN      
        1 catctggacg gttgctatga aaagaaaaga atttcgcaaa gaacaccaaa agaaacaccc
       61 ctatccacga tttcgcaccc gccgtgccat cttgcgaaac ggcatcgcat ttctggcttc
      121 aattttgctg aattataaag ttgaaggcca ggaaaacctg cccgaaaagg gtccattgct
      181 cattgttaac aaccatttcc actttttaga caccatcagc cccattcaca gcaccaaata
      241 tcctttggag ttcatcgggg accatgttat gcccaacgcg ccggtcgccc tgagggtttt
      301 gccaaggttg tggcgaacgc ttgaaattca gcaaggcacc gccaacctgg aagctattcg
      361 tgccgcggag gccattttgg cacaggatgg cattttggcg attatgcccg aaggtcatgt
      421 gcacaaaccg cccctgcgtg aagccttgcc cggcgcggcc tttttagcac ttcgcctggg
      481 cgtgcccatt ttagccatcg gcacctatag cgaagataac tgggatattt ttggcaccat
      541 acgcaacaaa aaacgcaaag cgcgcgtcat ttccaaaatc ggcaaagttt ttggcccgct
      601 aaaaatcgct gacggtggca accgcccctc cagagatgat gtcaaccgcg cccggcacga
      661 aatcatgagc caaatcgctg ccctcttacc gccggaagtg cgcggagaat ttggctaaga
      721 tctctcattt gctggcttga tattaacgcg ctttatacat tgatttgcta ttatgaacat
      781 gataatagct tgaattagca atggaattaa tatggaatat aaagattact acaaaacttt
      841 aggtgtttct aaaaccgcga ctgacgatga gataaaaaaa gcttatcgcg atttggcgcg
      901 caaatatcac cccgacgtga ataaagacct aaaagccgaa gagatgttta aagaagtcaa
      961 cgaagcctac caggtgcttt cagacagcga aaaacgccag aaatacgaca atttaggctc
     1021 ggaatggcaa caataccaac gcgcgggcgg ccagccgggt ggctttgact ggggacgctg
     1081 gcaacaacaa cccggacaag gcggcggtta ccgcacgatg acacccgaag agtttcagga
     1141 aatttttggc ggcatgggta tgcccggtgg tatgggcagt tttagtggaa tgggtggctt
     1201 ttctgacttt ttcacctcgc tgttcggcgg aggttttagt ggtgggttta gtccgggcac
     1261 agagaccagc tattcacaag gtggtcgcag ctaccgtacc caggcacccg cccgacgtga
     1321 tgttgaacat gagactgaaa tcacccttga agaagcctac cgtggcacgc aacgcagcct
     1381 taggttctct gatggacgca cgattaatgc cagcatccct gctggggtag ataccggttc
     1441 aaaagtacgc ctgagtggtc aagccgatgg cgcggacctg tatttgctca tcaaaatgct
     1501 cccgcacaaa gtttttaccc gcaaagggaa taacttgctg ctgacaattc cggtagattt
     1561 ttatactgcc gcgctgggcg gtgaggtgac ggtacccacc atggagaaac ccgttaaact
     1621 caacattcca gcgggaaccg atagcgggaa aacttttcgg ctcaaaggct taggcatgcc
     1681 ttccttgcgc aagtcagatc aaaaaggcga tatcctggcc aaagtgcaaa tacacttgcc
     1741 caaaccactt agccaggcag agaaaagcaa gctccaggcc ctgcgcgatg agtatcacag
     1801 ctaaggtcag tggggcattt aaagtttaaa accagccttg ttagagtgca aacatgcgaa
     1861 atctgtcaga tataactgac agatttctta tttcctgctt tttgtcagtt atatctgaca
     1921 ctttcttgat ttttttcatt ttgtcagtta taactgacac aagcgaggtg tgtcatgatt
     1981 atccgagaaa actatctaaa aaaaatcgaa ccatttattg acaaaccgat tataaaagtg
     2041 cttactggat tgcgcagaag cggaaagt
//
LOCUS       DAOSFC010000248         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_54372_length_2054_cov_4.705853, whole genome shotgun sequence.
ACCESSION   DAOSFC010000248 DAOSFC010000000
VERSION     DAOSFC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54372_length_2054_cov_4.705853"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..>2054
                     /locus_tag="PK791_06120"
     CDS             202..>2054
                     /locus_tag="PK791_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92923.1"
                     /translation="MKNLHVISHTHWDREWYHTFQQFRLQLVHLIDNLFVILDSDPNY
                     LYYMLDGQTVVLEDYLRMRPENTSKLKKYIQEGRILIGPWYVLPDEFLVSSEATVRNL
                     LVGKEICEQFGRRMMIGYLPDPFGHIGQMPQILRGFGIDTSALWRGVVPEMPTAFTWQ
                     APDGSQVLLGHLYYGYGNIAHWPVADKERSMQLLEDAVKRLEPHSNTSQYLMMQGTDH
                     LEPHPETSEHIRFYNENNPDKNIAIHSTLPAYFAALKQEIEEKGISLSVHIGELRDPQ
                     KAHMLPGVLSTRMWIKQRNWFAQTMLERWVEPFSVWAETLERGTDAFSPVTIHQGTPR
                     IANPASLIRESWRTLMKNHAHDSICGCSVDETHEDMVPRFDKVDELNKHLLNQSTNAI
                     LSHITTTAPEGVEAPVLAITVFNGAPVGFSGMVQTDITIPFDDDFDLRDENGNLIEKS
                     LTFSDYASFEEHIYTIANLSTRFEQVNESGYNGKHLLDFELKFDKEEDIWCIHTIVSD
                     IIPPDEALLESKTKKVIKKLQKLDPNTPVKVIIFAGIPGNLVFYAKDVPAFGYKTYWL
                     CFSDSVETEDELDFEPVADDEIAELENEWLKISLDRFDGSFSILDKRNGAV"
BASE COUNT          544 a          485 c          484 g          541 t
ORIGIN      
        1 cctggagatt ctccagtctg ccatcgtgca cgaagcagac cccatttgca aagatgccat
       61 gcagtgggcg attgaagttg tccgccaacg tttatccatg ccataaaacc atctctgcaa
      121 tagcttttac cagcgttttt cttgtgatgt catcagaata tggcataatt aagccatcaa
      181 ctttttgcgc ggaggcttcg catgaaaaat ttgcacgtta tctcacatac tcactgggac
      241 agggagtggt accatacttt tcaacaattt cgtctgcaac tggttcacct cattgacaat
      301 ctttttgtca ttttagattc cgaccccaac tacctgtact atatgttgga tgggcaaacc
      361 gtcgtgcttg aagactatct gcgtatgcgc ccggaaaaca ccagcaagct taaaaaatac
      421 attcaggaag gtcgcatttt aatcggcccc tggtatgttt tacccgacga atttttggtt
      481 tcttctgaag cgactgtgcg caacctcttg gttggcaagg agatttgcga gcaatttggg
      541 cgaagaatga tgattggtta cctgcccgac ccatttggtc atatcgggca gatgccgcag
      601 attttgcgcg gttttggcat tgatacaagc gctctgtggc gcggtgtcgt gccggagatg
      661 cccaccgcgt tcacctggca agcccccgat ggcagccagg ttttgctggg tcatctctat
      721 tatggctatg gcaatatcgc gcactggccc gtggctgata aggaacgttc catgcagttg
      781 cttgaggatg ctgtcaagcg tttggagccc cacagcaaca cgtcgcagta cctgatgatg
      841 cagggcactg accatctcga accccatccg gaaacatcgg agcatatccg gttttacaac
      901 gaaaacaatc ccgataaaaa cattgccatt cactccacct tgcccgctta ttttgcagct
      961 ttgaaacagg aaattgaaga aaaaggtatc tctttgtcgg tgcatatcgg cgagttgcgc
     1021 gacccgcaaa aagcgcatat gctgcccggc gttttgagca cgcgcatgtg gattaagcaa
     1081 cgcaactggt tcgcgcaaac gatgctggag cgttgggtgg aaccctttag cgtttgggcg
     1141 gaaacgcttg aacgaggcac agacgccttt agtcctgtaa cgattcatca aggcacgcca
     1201 cgcatcgcca acccggcttc attaattcgt gaatcctgga gaactttaat gaaaaaccac
     1261 gcccacgact cgatttgtgg ctgttcggtt gacgaaacgc acgaggatat ggtgccgcgc
     1321 ttcgataaag tggatgaact taacaaacat cttcttaatc agtccacaaa tgccattctc
     1381 tcgcatatca ccacaactgc accagagggt gtggaagccc ctgttttggc gattacggtc
     1441 tttaatggcg cgccggtcgg gttttcgggc atggtacaaa ccgatatcac gattccattt
     1501 gatgacgact ttgatttgag agacgaaaac ggaaatctta tagaaaaatc cttaactttc
     1561 tcggactatg ccagctttga agaacatatc tacaccatcg ctaatctaag cacgcgattt
     1621 gagcaagtca atgaaagcgg ttacaacggc aaacacttgc tggattttga acttaaattt
     1681 gacaaagaag aagatatttg gtgtattcac accattgttt cagatatcat cccacccgat
     1741 gaagcccttc tggaatctaa aacgaaaaaa gtgattaaaa agctccaaaa gcttgaccca
     1801 aatacacccg tgaaagtgat tatctttgca ggaattccgg gtaatttggt cttctacgcc
     1861 aaagatgtgc ccgcttttgg ttataaaact tattggctgt gctttagcga ctctgtagaa
     1921 acagaagatg aacttgattt tgaaccagtg gctgacgatg agattgcgga gctggaaaac
     1981 gaatggctga agataagttt ggatcgcttt gatggcagtt tcagcatttt ggacaaacgc
     2041 aatggcgcgg ttta
//
LOCUS       DAOSFC010000249         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_54791_length_2044_cov_4.018100, whole genome shotgun sequence.
ACCESSION   DAOSFC010000249 DAOSFC010000000
VERSION     DAOSFC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54791_length_2044_cov_4.018100"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PK791_06125"
     CDS             complement(<1..268)
                     /locus_tag="PK791_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOH92924.1"
                     /translation="MTKQKNEGFSLEKVLRKTFKGLLDGVASFFLKIGLTPNAVTLLG
                     LAGSIGAAVLIALGMPLWGGVVLLLMAPLDAVDGAMARLSGRSSK"
     gene            complement(272..>2044)
                     /locus_tag="PK791_06130"
     CDS             complement(272..>2044)
                     /locus_tag="PK791_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOH92925.1"
                     /translation="WAARKAGLKQLPSGKAQSVAALQEPADLMALPERVEDAPVEVVE
                     ETVIRRPRPVVEKPQENVPEEVVTRIRASSRRAQEQKSKQQAAERHANLPPLSLLAAD
                     KIYTENQATINMNAGLIEQTLAEFGVPAKVVGYRVGPTITQYAVEPGYLDKAGDEKQK
                     VRISQISSLSRDLALALKAERLRIEAPVPGESYVGVEVPNTQVSTVRLRALLESPEFL
                     RMKSPLALALGRDVSGAPVVSDLSSMPHLLIAGATNSGKSVCIQAITLNLIMNNHPDD
                     LRLVMIDPKRVELNRFNGLAHLIGQVETNPERIMAVMRWATTEMDARYEKLEKVQARN
                     LDNFNNRMRKSGKETLPRIVIMIDELADLMMSAGAEIENALVRLAQKARAVGMHLIVA
                     TQRPSIEVVTGLIKANFPTRIAFSVASGVNSRVILDENGAEDLLGRGDMLFLHPSKGV
                     PQRAQGAMVSDKEIQNVMDWWAQNVGSTSQSSLFDIASSDVSQKSPEKSAEESETEEA
                     PWEAIVQEESEKDSDEAIIQEAIALVRKQQRASASFLQRQLRIGYPKAAWLIDQLEAR
                     GVIGEAQGGGREREILPEEDEEKE"
BASE COUNT          459 a          620 c          443 g          522 t
ORIGIN      
        1 acttcgaact tcttcccgac agacgcgcca tcgcgccatc aaccgcgtcc agcggtgcca
       61 tcagcaacaa aacaacgcca ccccacagtg gcattcccag ggcaatcaag accgcggctc
      121 caattgaacc tgccaatccc agcaaagtta ccgcgttagg cgttaaacca atctttaaga
      181 aaaagctggc gacaccgtcc agcaaacctt taaaggtctt acgcaagacc ttctcaagac
      241 taaaaccttc atttttctgt ttcgtcatgc cttattcttt ttcctcatct tcttcaggca
      301 aaatttcgcg ttcccggcca ccgccctgcg cttcaccaat caccccgcgg gcctcaagct
      361 ggtcaataag ccaggccgcc tttggataac ctatgcgcaa ttgacgttgc aggaaagacg
      421 cgctggcacg ttgttgtttg cgaaccaaag cgattgcctc ctgaataatg gcttcatcgc
      481 tgtctttttc gctttcttcc tgcacgattg cttcccaggg ggcttcttca gtttcgcttt
      541 cttccgcgct tttctcaggg ctcttttgcg aaacatcaga gctggcaata tcaaacaagc
      601 tgctttgact tgttgaacca acattttgcg cccaccagtc cattacattt tgaatttctt
      661 tgtcgctcac catcgcgccc tgggcacgtt gcggaacgcc tttggatggg tgcaaaaaca
      721 gcatatcacc tcttccaagc aggtcttccg cgccgttctc atccaaaatc acgcgtgagt
      781 tcaccccact ggcaacacta aacgcaatac gtgttgggaa gttggcctta atcaaacccg
      841 taaccacttc tatgcttggt cgttgcgttg ccacaatcaa atgcatcccc accgcgcgcg
      901 ctttttgtgc caaacgcacc aaagcgtttt cgatttctgc tccagcgctc atcatcaggt
      961 cggccagttc atcaatcata atcacgatgc gtggcagggt ctctttcccg cttttgcgca
     1021 tgcggttgtt aaaattgtca agattacgcg cctggacttt ttcgagtttt tcatagcgcg
     1081 cgtccatttc ggtcgttgcc cagcgcatca ccgccataat gcgctctgga tttgtctcca
     1141 cctgtccaat caaatgggcc aatccattaa agcggttaag ctcaacccgt tttgggtcaa
     1201 tcatcaccaa tcgcaggtca tcaggatggt tattcataat caggttaagc gtgatggctt
     1261 gaatacacac cgatttaccg gagttcgtcg cgccagcaat caaaagatgg ggcatactgg
     1321 aaagatccga tacaaccggc gcgcccgata catccctgcc cagggccaaa gccagaggcg
     1381 atttcatgcg caaaaattcg ggggattcaa gcaaagcgcg caaacgcacc gtgcttacct
     1441 gcgtatttgg cacttccaca cccacgtaag actcacccgg cacaggcgct tcaatgcgca
     1501 agcgttccgc tttcagggca agcgccaggt cgcgcgatag cgaagatatc tgcgaaatac
     1561 gcactttttg tttctcatcg cccgccttat ccaaataacc tggctccacg gcatattgcg
     1621 tgatggtagg cccgacccga tagccgacca ctttggcagg cacgccaaat tccgcaagcg
     1681 tttgctcaat caagcccgcg ttcatattga ttgtggcttg gttctcagta tagattttgt
     1741 cagcggctaa taaacttagc gggggcaaat tagcatgtct ttcagccgct tgctgtttgc
     1801 ttttctgctc ttgcgctctg cgcgatgagg ccctaatccg cgtaacgact tcttccggaa
     1861 cgttttcttg tggcttctcc accactggac gcggacgacg aatcaccgtc tcctccacaa
     1921 cttctacagg cgcatcttca acacgctctg gcaatgccat taaatctgcc ggctcctgca
     1981 aagcagccac actctgggcc tttcccgatg gcagttgctt caaacccgct tttcgcgctg
     2041 ccca
//
LOCUS       DAOSFC010000250         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_54940_length_2040_cov_5.131486, whole genome shotgun sequence.
ACCESSION   DAOSFC010000250 DAOSFC010000000
VERSION     DAOSFC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54940_length_2040_cov_5.131486"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1584
                     /locus_tag="PK791_06135"
     CDS             160..1584
                     /locus_tag="PK791_06135"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HOH92926.1"
                     /translation="MTSIDASIIITVKVADISESHETKISFHNIESDLNTAFQVGRRQ
                     STAVLFDLLESNLHAFICSYKNWKNVGTDKRSVLMEAGTITYRRHIYLDEQGNRRKPF
                     DELIGLQAYQRNSNKVETMVASLATVSSFRDVAELFSYVTKEYISPSTVYRMTGRVGK
                     KLRAIEQMEDEQNIEPGTIKTNRLMAEADGVIIRLQGEAQKRAEVKVAVFYTGKTPIA
                     LHRNRLLNKFMTCQLGMSNEAWQKHLSTFADRTFKLSDVAFAQIGGDGAKWVRNSFEY
                     LGVPMLHLLDRFHVIRSLRSAFAQHLDLKETLAKLFSEGFTAVEADLLEIISNSKGAV
                     REQQIKCFDYLRSNQDAITDLDKRGFGDLGFCSMGAMEGNVGKLIAKRMKGHGRSWSI
                     QGAQNILAVIRYKNLIKTEAFAMTAMPVPEPQSRWRYQRYKAPEWTPNSASIPVFSST
                     HATEPWVQLFKSKVNGSLSINAFF"
     gene            1725..>2040
                     /locus_tag="PK791_06140"
     CDS             1725..>2040
                     /locus_tag="PK791_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOH92927.1"
                     /translation="MKDFSKLTKNLEHFGYTVRAFATVEEANLYLTEAIQNHSVGFGG
                     SKTLEAMGLQNLLSERNEVYWHWYPQENIGRAETLKKASGAEIYISSVNAIAETGEII
                     NID"
BASE COUNT          646 a          394 c          465 g          535 t
ORIGIN      
        1 ccacgcgcca gctggatatc tattcctgcg agcgttctgc taaatcgtgt ttatgtatga
       61 gggtttctgt ttcaaaagtc gatagtttca aaataaggaa aaaaagagag ttcctgtcat
      121 aattgaattt gtgagaaaac aaaaaggagg aactctcaaa tgacatctat cgatgcctca
      181 ataattataa cggtaaaagt ggcggatatt agcgaaagtc atgaaactaa aatatccttt
      241 cataacatag aatcagatct caatactgct tttcaagtag gaagacgaca gtcaacagct
      301 gttctgtttg atctacttga aagcaatctt cacgctttca tttgcagtta taaaaactgg
      361 aagaatgttg gtactgataa acgatctgtc ttaatggaag caggtacaat cacctatcgc
      421 aggcatattt acttagatga acaaggtaat cgccgtaaac cctttgatga gcttatcgga
      481 ctgcaagcct atcaacgtaa cagcaataaa gttgaaacta tggtagccag cctggcaact
      541 gttagtagct ttcgcgatgt ggctgaattg ttttcatatg tcaccaaaga atatatcagt
      601 cccagtacag tttatcgaat gactggacgt gtaggcaaaa agctgagagc catagaacaa
      661 atggaagatg agcaaaacat tgaaccgggc accatcaaaa caaaccgatt gatggcagaa
      721 gcagatggcg tgattatccg tctccagggc gaagcacaaa aacgggcaga agtgaaagta
      781 gccgtgtttt atacggggaa aacccccatt gctctccatc ggaaccggtt attgaacaaa
      841 tttatgacct gtcagttagg tatgagcaat gaagcatggc aaaagcattt gagtactttt
      901 gctgacagaa ctttcaaact cagcgatgtg gcttttgcgc aaattggcgg agatggagcg
      961 aaatgggtac gcaactcctt tgaatatctt ggtgtgccta tgttgcattt actggatcgt
     1021 ttccatgtta ttcgaagtct tagaagcgct tttgctcagc atttggactt gaaagaaact
     1081 ctggcaaaac tctttagcga aggctttact gctgttgaag ctgatttgtt agaaatcatt
     1141 tccaacagca agggtgctgt cagagaacag caaataaaat gctttgatta tcttagatcc
     1201 aatcaggacg ccatcacgga tttggacaaa cgcggttttg gtgatctcgg cttttgtagc
     1261 atgggtgcta tggaaggtaa tgtgggtaaa ctgattgcca aaagaatgaa agggcacgga
     1321 aggtcatggt cgatacaggg tgcgcagaat atcttagcgg ttatcagata caaaaacctg
     1381 atcaaaaccg aagcttttgc aatgacagca atgccagtgc ctgaacccca aagccgatgg
     1441 agatatcagc gttacaaagc tcctgaatgg acaccaaatt cagcttcaat tccagtcttt
     1501 tccagcacac acgcgacaga accttgggta caattattca aaagcaaagt caatggcagt
     1561 ctctctatca atgccttttt ctagattcta tcgaaaagtg aaacagaacc ttagggtgtc
     1621 tgagcatgat aagcattgcg aagggggcta ttctaaagta tcatcacagt aaggctcatg
     1681 atggcttgag cttttgacgc aaatgaatga ggataatgat aaccatgaaa gatttcagca
     1741 aactgacaaa aaaccttgaa cattttggct ataccgtgcg cgcctttgcg acagtagaag
     1801 aagccaatct ttatttaacc gaagctatcc agaatcatag tgttggcttt ggtggctcta
     1861 agacccttga agcaatggga ttacagaact tgctttcaga gcgaaacgag gtctattggc
     1921 actggtatcc gcaagagaat attggcaggg ctgaaacttt aaagaaagcc agcggggctg
     1981 agatctatat cagctccgta aacgctattg ctgaaacggg tgaaatcatc aacattgatg
//
LOCUS       DAOSFC010000251         2036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_55111_length_2036_cov_5.065119, whole genome shotgun sequence.
ACCESSION   DAOSFC010000251 DAOSFC010000000
VERSION     DAOSFC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55111_length_2036_cov_5.065119"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="PK791_06145"
     CDS             complement(<1..1585)
                     /locus_tag="PK791_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92928.1"
                     /translation="MAFYPVLFYLTRLVLPRLQIGINKLILFLLLTLIFNLWQFRKSW
                     KENIKFEKKDLFPAALFVLILVTRLIPAWQYPYPAWTDSLHHSIITKLVMLNGKLPDT
                     MLPFDPASLKGYHLGLYSLTGSLSLLSNVAPHIALLYYSQITNALAGLAVFIFLDKKV
                     GRIAALIGMVFVGLISFQPALYFNWGRFTQLASQTMLFPAALLVWMAIEPDSVHSLTA
                     SCQKKRPIVEIILAALLISGTALLHFRVAVFFLPLLGLIVLFTAFENKLSPKERKRVV
                     LKTVLIALATIILTLPAIIPALQSYLAPTEPTVGSSVAVSPDKTDMPLSEHPYYKFEY
                     SVFYDLGLTKTASLFTVLGLLLGLLFKKTRTMALLTIAWVATLLIEGNFHKFNIPKLA
                     FVNMTGVMIIAYLPGAVGFGLLFETLKESTSRLVGSNPARRNIQPILYWVIAFIAILF
                     ISDRINTIEPFRHFMSDDDRQAMVWIKENTPPDSIIAINTQYWAGGAFHGTDAGYWIP
                     YFAERDTTTRTMMSVDAPDY"
BASE COUNT          649 a          403 c          435 g          549 t
ORIGIN      
        1 cataatctgg cgcgtcaacc gacatcatgg tgcgcgttgt cgtatcgcgt tctgcaaaat
       61 aggggatcca atagcctgca tctgtgccat gaaaagcccc gccagcccaa tattgagtgt
      121 ttatcgcaat aatggaatct ggtggcgtat tttctttaat ccaaaccata gcttgtcggt
      181 catcatcgct cataaaatgg cgaaatggct caatagtgtt tatgcggtct gagatgaaaa
      241 gtatagcgat gaatgctatc acccaatata gaataggttg gatatttctc cgcgctggat
      301 tagatccaac cagacgtgat gtggattctt tcagagtttc aaacagcaat ccaaagccga
      361 ccgctcccgg taaataagca attatcataa cgcctgtcat atttacaaag gcaagtttgg
      421 gaatgttaaa tttgtgaaag ttaccttcaa ttaacagcgt cgcaacccat gcaatggtta
      481 gcaacgccat cgttcttgtt ttcttaaaca agagcccaag taataagccc aatacagtaa
      541 ataaactagc ggtttttgtt aatcctaaat catagaagac agaatattcg aacttataat
      601 aaggatgttc tgaaagtggc atgtctgttt tatcaggtga aacagcaact gaagaaccaa
      661 ctgtgggttc agtgggtgcg agataggatt gcaaagctgg aatgattgca ggcaaagtga
      721 ggatgatagt tgccaatgct atcaaaactg ttttcaatac gacgcgtttg cgttcttttg
      781 ggctgagttt attttcgaaa gcagtaaaaa gaacaattaa gcctaaaagg ggcaaaaaga
      841 aaacagccac acggaaatga agcaaggcgg tcccgctgat caaaagggct gcgaggataa
      901 tttcaacgat tggtcttttc ttctggcagg atgctgtcaa tgaatggaca gagtcaggct
      961 caatagccat ccacaccaaa agcgcggcag ggaaaagcat agtctgggag gctaattggg
     1021 taaagcgtcc ccagttaaaa tacagcgctg gttggaagct aatcagaccg acaaaaacca
     1081 tgccaatcaa agcggcaata cgcccaactt ttttatccag gaatatgaat accgctaaac
     1141 ctgccagggc gttcgttatt tgtgagtaat aaagtaacgc tatatgagga gcaacattgg
     1201 ataacaaact aagagatccg gtcaaactgt ataatccaag atgataacct tttaaactgg
     1261 cggggtcaaa aggaagcatt gtgtctggca atttaccgtt gagcataacc agttttgtta
     1321 tgatgctatg gtgcaagctg tccgtccagg caggataagg atattgccag gcgggaatga
     1381 gccttgtgac aagtatcaat acgaataaag ctgcagggaa taagtctttc ttctcaaact
     1441 taatattctc tttccagctt tttcgaaact gccaaagatt aaaaatgaga gttaatagca
     1501 aaaagagaat gagtttattg atgccaattt gcaacctggg aaggactaag cgcgtaaggt
     1561 aaaaaagaac cggataaaat gccatgccga ggaagtacgc caaaatccag cggtgcaaag
     1621 tcttgaaacg tttccaacac ccacctaaac ttagcaaagc ccagcctggc aaaagttgca
     1681 ttgttaaggt gatgataatg agtttgagta aagttaaaaa ggtcattcta agccctgttt
     1741 ctctttagaa gttatctctc attataagct atttacgctt gaattcaccc aatagattaa
     1801 taaagtctca gtttctcact ttaattcata atggcatttt caacagtaaa ttttccttcg
     1861 cactaaaatt tgaactcttg tttgttatct taattagctg tcctgtctta agaattaaaa
     1921 aagacgctcg tctgaacgtc ttttttaatt aagttaagca aaacacatga aagctgaaag
     1981 gtttcagctt cctggtcccc catcaattgt cgcgattctt tcagccctcc ctaacg
//
LOCUS       DAOSFC010000252         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_55314_length_2031_cov_4.780364, whole genome shotgun sequence.
ACCESSION   DAOSFC010000252 DAOSFC010000000
VERSION     DAOSFC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55314_length_2031_cov_4.780364"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /gene="tyrS"
                     /locus_tag="PK791_06150"
     CDS             complement(<1..1072)
                     /gene="tyrS"
                     /locus_tag="PK791_06150"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOH92929.1"
                     /translation="MNIDEQVNYLMRGAEYGDQQVFDAMADELRERLIESEKTGVPLK
                     VYCGFDPTSSDLHLGHTVPLRKLAQFQELGHDVTFVIGTFTALIGDPSARDKSRPLVG
                     LETILENAKTYQEQAFRVLDPEKTRIVYNGDWLNNIPYHEIFNVLTNFTIQQMLSREG
                     LQKRWESNEPIYLHEMLYPITQGLDAHHLEADVQVGGTDQLYNVITASRKVMQSYGQR
                     PNIGILMPLLPGTDGVQKMSKSLGNYIPINTTAEDMYGKVMSIPDDVMAQYAKLVTKW
                     SSQELYTFLGALETNSIHPRDAKMQLAYAITSAFYGDSLADEAENRFVTTFQKGDIPD
                     EMPEVHLEAGKHTVLDVLVDQGL"
     gene            complement(1224..>2031)
                     /gene="lgt"
                     /locus_tag="PK791_06155"
     CDS             complement(1224..>2031)
                     /gene="lgt"
                     /locus_tag="PK791_06155"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOH92930.1"
                     /translation="TTMDYLKNPLLILQIWKGGLGIPGGVIGGVLALFIYTKVKKESF
                     LEWADYIAPGLLVAQGIGRWGNYINQELYGKPSNLPWAIEISPEYRYPGFENISHYHP
                     LFFYEFVLNILAAVILLLIDRNFKKKLYKGDIFLLYLVAYPAIRFGLEFVRLVPSLSK
                     SGVNINQTVMLIVMILAIVVFVLRHTFFKPKQSEELAEEEVYLDETVEEEQAEDLEEL
                     DSLDADLSEIPEGTEETEEPDAEETGEAETQELPDETENEPEPEGEDEEN"
BASE COUNT          548 a          500 c          424 g          559 t
ORIGIN      
        1 ccaggccctg atcaaccaaa acgtccaaaa ccgtatgttt gccagcttcc aaatggactt
       61 caggcatttc atcaggaata tcaccctttt ggaaagtcgt cacaaagcgg ttttctgctt
      121 catctgccag cgaatcgcca taaaaagcgg aagtgatcgc ataagccagc tgcattttcg
      181 catcgcgcgg atggattgaa ttcgtctcaa gcgcgcccag gaaagtgtaa agctcctgcg
      241 acgaccattt ggtgaccagc ttggcatatt gcgccatgac atcatcagga atgctcatca
      301 ctttgccata catatcttcc gcggtggtgt tgatgggaat gtagttgccc aaagatttgc
      361 tcatcttttg cacgccatcc gtgccgggca ataagggcat taaaatacca atgttcggac
      421 gctgcccata gctctgcatc actttacgcg aagcggtgat gacgttgtaa agctgatccg
      481 ttccacccac ctgcacatcc gcttccaaat gatgcgcgtc aagcccctgg gtgatggggt
      541 aaagcatttc gtgcaggtag atcggctcgt tggattccca gcgtttttgt aagccttcgc
      601 ggctaagcat ttgctgaatg gtgaagtttg tcaaaacgtt gaaaatctca tggtagggaa
      661 tattattcaa ccagtcgcca ttgtaaacaa tgcgcgtctt ttcagggtca agcactctaa
      721 aagcctgttc ctgataagtt tttgcgttct caagaatggt ttccaatcca accaaaggac
      781 gcgatttatc ccgtgctgat ggatcgccaa tcaaagccgt aaaggtacca atgacaaagg
      841 taacgtcatg ccccagctcc tggaattgtg ccaacttgcg caaaggcacc gtatggccca
      901 aatgcaggtc agatgatgtc gggtcaaagc cacaataaac tttgagtggc acaccagttt
      961 tttctgattc gattaaacgt tcgcgaagtt catcggccat cgcgtcgaaa acttgttggt
     1021 cgccatattc agcaccacgc atcagataat taacttgttc atcaatgttc attcttgcct
     1081 ccatcatact gactctttta atcggcgttg agtcataact tattcttcct gaaattttac
     1141 cactaaccag gtaaattaaa taaaaccacc ccgagaaatc aacaattttt cctggggtgg
     1201 ttagatttta tgtgaacaaa aggctaattt tcttcgtctt cgccttcagg ctcgggctca
     1261 ttttccgttt catcagggag ttcttgagtt tcagcctcac cggtttcttc cgcatcaggt
     1321 tcttcagttt cttcagttcc ttcaggaatt tcgctcaggt ctgcgtccag actgtcaagt
     1381 tcttccaaat cttccgcttg ttcttcttca acggtttcat ccaaataaac ttcttcttcg
     1441 gcgagttctt cactttgttt tggtttgaag aaagtatggc gcagaacaaa tacgacaatc
     1501 gccaaaatca tgacaattaa catcactgtt tggttgatat taacgccact tttgctcaat
     1561 gatggcacca ggcgcacaaa ctccagtcca aagcgaatgg cgggataagc aaccagatac
     1621 aagaggaaaa tatcaccctt atagagtttc tttttgaaat tacggtcaat tagcagcaaa
     1681 ataacagctg ccaggatatt caaaacaaac tcatagaaga acaggggatg ataatgtgaa
     1741 atgttctcaa acccaggata gcggtattcg ggtgaaatct caatcgccca gggcaaatta
     1801 ctcggtttgc cataaagttc ctgattgata tagttacccc agcgaccgat accttgcgct
     1861 accaacaatc caggcgcgat atagtccgcc cattccaaaa agctttcttt tttaactttg
     1921 gtgtagatga acagtgccag cacgccacca atcacaccac cggggatacc cagaccacct
     1981 ttccaaattt gcaggataag caaggggttc ttcaaataat ccatcgttgt g
//
LOCUS       DAOSFC010000253         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_56227_length_2009_cov_4.185773, whole genome shotgun sequence.
ACCESSION   DAOSFC010000253 DAOSFC010000000
VERSION     DAOSFC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56227_length_2009_cov_4.185773"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /gene="argS"
                     /locus_tag="PK791_06160"
     CDS             complement(<1..751)
                     /gene="argS"
                     /locus_tag="PK791_06160"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOH92931.1"
                     /translation="MMPFNEQKELLKIIDQFCLEQNLPAGKIEFRDIPFSGEWGIAAP
                     LFPLAAADPNRSGPVPQHAQTLAEKLKDYVGLPAGFSRIEALKGYLNLYYETSQYAKK
                     VIDYVHYADHCYGMAEPTGKTVMVEYSNPNTHKPLHVGHLRNVILGSSVCNILEASGD
                     KVIRANYIGDIGLHVIKWMCNYEMYHAGQAPSADIMRWMADLYTEADRRFHDEPGFEE
                     ACRAYFNRWDEGDKDIEALWLKTRQWSMDGFK"
     gene            complement(909..1166)
                     /gene="rpsT"
                     /locus_tag="PK791_06165"
     CDS             complement(909..1166)
                     /gene="rpsT"
                     /locus_tag="PK791_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOH92932.1"
                     /translation="MKSQIKRNKQNQARRLRNRVYRGSARTYLRKAMASINAEDLEKS
                     KADVARAIKALDKAAGKGVIHKNNAARRKSRLMAHYNKLQA"
     gene            complement(1286..>2009)
                     /locus_tag="PK791_06170"
     CDS             complement(1286..>2009)
                     /locus_tag="PK791_06170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOH92933.1"
                     /translation="LGRLANGTNRRFDIKEGDTVVLSSHPIPGNEELVYRAINRLMER
                     GADVIYEAIAPVHVSGHASQEEIKLLLHLVKPKYLIPVHGETRMLKQHKKLALSVGME
                     ESQIALVENGRVIEFKNGKMKLGERIPGGYVFVDGTGVGDVDRDIMRERELLSQDGVV
                     LLNLILDKKTGVYKDTEIISRGFMSQDESVDLFDNLKKRAEKLAQYPSSNLQKELQSV
                     AKSLLFDETRRRPLIFVNISRE"
BASE COUNT          515 a          501 c          427 g          566 t
ORIGIN      
        1 gcttgaaacc atccattgac cactggcgtg tcttcagcca gagagcttca atatccttat
       61 cgccttcgtc ccagcgattg aaataagccc ggcaggcttc ttcaaaccca ggctcatcat
      121 gaaaacgtcg gtccgcctca gtgtaaagat ctgccatcca acgcatgatg tccgctgatg
      181 gcgcctggcc ggcgtgatac atctcgtaat tacacatcca tttgatgaca tgcaagccga
      241 tatcgccgat gtaattagcg cggataacct tatcgccact ggcctcaagg atattacaca
      301 ccgaactgcc caggataaca ttgcgcaaat gccccacatg gagaggtttg tgcgtgttgg
      361 ggtttgaata ttccaccatc accgttttgc ccgttggctc ggccattccg tagcagtggt
      421 cggcatagtg tacgtaatca atcacctttt tggcatattg actggtttcg taataaaggt
      481 ttagataacc ctttagcgct tcaatgcgtg aaaagcccgc gggaagaccg acatagtctt
      541 tgagtttttc agccaaagtt tgggcatgtt gtggcacggg accacttcgg ttggggtcag
      601 cggctgccag ggggaacaaa ggcgcggcga tgccccattc gccactgaat ggaatatcac
      661 gaaattcgat tttgcctgca ggcaagtttt gctcaaggca aaactggtca ataattttta
      721 agagttcttt ttgctcgtta aatggcatca taaattctca ccaaatgtta attatatcat
      781 ctcatgtcgc ttaaaacggg ttttcccaaa attcttataa tccaattaca aaagcactta
      841 aaaggaaaaa gcgaggatga cctcgctcca ttctgcgtaa cgatagactc aaaacgagtc
      901 ttagtcaatt atgcttgcag tttattgtaa tgtgccatca agcgggattt gcggcgcgcg
      961 gcattgttct tgtggataac acctttgcca gctgctttgt caagcgcctt aatcgctcgg
     1021 gcgacatcag ctttggactt ttcgagatct tcagcattaa tcgatgccat tgctttgcgc
     1081 aaataagtgc gcgctgaacc acgataaacg cgattgcgta aacgtcgcgc ctgattttgt
     1141 ttattccgct taatttgaga cttaatatta gctaattctt cctccgtatt atactttcat
     1201 acacgaactt ttattttaca acatatttct aaaatgtcca gtattaaaac ttgttttaag
     1261 agcttgtttt cgcaaaaacc cacgcttact cccgcgaaat gttcacaaaa atgagcgggc
     1321 gacggcgggt ttcatcaaaa agcaacgatt ttgcgacact ttgaagttcc ttttgcaagt
     1381 tactgctcgg atactgggcg agtttttccg cacgtttctt caggttatca aacaaatcca
     1441 ccgactcatc ctgcgacata aacccacggc tgatgatttc agtatctttg taaacgccgg
     1501 ttttcttgtc caggatgaga tttagcaaaa ccacaccatc ctggctcaaa agctcccgtt
     1561 cgcgcatgat atcgcggtca acgtcaccca caccagtacc atccacaaag acatatccac
     1621 cagggatacg ttcgccaagt ttcatcttgc catttttaaa ctctatcacc ctgccatttt
     1681 ctaccagggc aatttgagat tcttccatac ccacgctaag cgccagcttt ttgtgctgtt
     1741 ttaacatacg cgtttcgcca tgaacaggga tgagatattt cggcttaaca agatgcaaca
     1801 agagtttgat ttcttcctgg ctggcatgtc cggaaacatg caccggcgca atcgcttcgt
     1861 agatgacatc cgcgccacgt tccatcaatc ggtttatagc gcggtaaaca agttcttcgt
     1921 tgccagggat gggatgcgaa gatagcacca ccgtatcgcc ttctttgatg tcaaaacgac
     1981 ggttggtgcc atttgccaga cgtcctaat
//
LOCUS       DAOSFC010000254         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_56804_length_1996_cov_5.535806, whole genome shotgun sequence.
ACCESSION   DAOSFC010000254 DAOSFC010000000
VERSION     DAOSFC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56804_length_1996_cov_5.535806"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK791_06175"
     CDS             complement(<1..258)
                     /locus_tag="PK791_06175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH92934.1"
                     /translation="MATPFLSIIIPAHNEATRLPQTLASVQNYLASKDFDAEVIVIEN
                     ASDDETAQIVRDWQAKMPNLRLISREEAGKGGAIKQGMLEAR"
     gene            complement(296..369)
                     /locus_tag="PK791_06180"
     tRNA            complement(296..369)
                     /locus_tag="PK791_06180"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Pro,seq:tgg)
     gene            521..919
                     /locus_tag="PK791_06185"
     CDS             521..919
                     /locus_tag="PK791_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH92935.1"
                     /translation="MYQKLIIVGNLGRDPEMRFTPDGNPVTSFSVATSRRYKDTDETT
                     WFRISVWGRQAESCNQYLKKGSKVLVEGRLYPDPQYGGPRVYQRNDGSWASSYEVTAE
                     TVRFLSSRSDSAGGGSDYMDDSMDDDEIPF"
     gene            929..1243
                     /locus_tag="PK791_06190"
     CDS             929..1243
                     /locus_tag="PK791_06190"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOH92936.1"
                     /translation="MADIKKITITVPEDLPATYVNMSYISHTPQEFILDFAGMLPGIK
                     PKISTRLVFSPLGAKLFLQALTENLLRYEKNFGEIKIPRGHSLADQLFRTADPKDPSE
                     EG"
     gene            1245..1895
                     /locus_tag="PK791_06195"
     CDS             1245..1895
                     /locus_tag="PK791_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HOH92937.1"
                     /translation="MKNLEKICEQIHEEFNAKTDQREQALKNARELIRRASLTIRAIH
                     RGDVQQAQEELQQGQVIVDDILQHLKREHPDLYYTGYTQDALKEFCEAKLTAAIIYDQ
                     PIPSPNEMGIDLPAWLNGLTETIGEMRRRCLDILRTGYTDEVERLLFNMDEIFTQLVT
                     MDYPDAVTDGLRRRTDLARGIIERTRADITTAYRQEMLTKKIDEFTELLADHETNQ"
BASE COUNT          550 a          481 c          493 g          472 t
ORIGIN      
        1 gcgggcttcg agcatgccct gttttatcgc accacctttg ccggcttctt cgcgcgaaat
       61 caagcgcaga ttaggcattt tcgcttgcca gtcgcgcacg atttgcgcgg tttcatcgtc
      121 gctggcattt tcgatgacaa tcacctcagc gtcaaagtcc ttgctggcga gataattttg
      181 caccgaagca agcgtttgtg gcaggcgcgt cgcctcgtta tgggcgggaa tgataatgga
      241 aagaaaaggt gttgccatag ctttgtagcc taagtatacc ttttaaacaa aaaagtcggg
      301 gtggtcggac ttgaaccgac gacctctgcg tcccaaacgc agcgcgctag ccgtctgcgc
      361 cacaccccgg cgcgagattg aagtatatcc tattgccttc gaaaacgtca agcgttttgt
      421 tgtggatttg aaaagcatca aaaaaagttt ccataacaga gctttttcta gtaaaattat
      481 agctgaagat tcgacacaaa agcgaaagga ttttacaaag atgtatcaga aacttattat
      541 cgtaggcaat ttaggacgtg acccagaaat gcgattcacg ccagacggaa acccggttac
      601 cagctttagc gttgcgacca gccgccgtta caaagatact gacgaaacca cctggttccg
      661 catttcagtt tggggcagac aagccgaaag ctgcaatcag tatctcaaga aaggttccaa
      721 agttttggtt gaagggcgcc tgtaccccga tccacaatat ggcggaccac gcgtttatca
      781 gcgcaacgac ggaagctggg caagcagcta cgaagtaacc gcggagacgg tgcgcttctt
      841 gtcatcccgc tctgattctg ccggtggtgg aagtgattac atggacgatt ccatggatga
      901 tgacgaaatc cccttctagg tcgcagtaat ggcggatatc aaaaaaatta ccattaccgt
      961 accagaagac ttgcccgcaa cgtatgtgaa tatgtcgtat atctcacata cgccccagga
     1021 atttattctc gactttgcag gtatgttgcc gggaataaag cccaagataa gtacacgact
     1081 ggttttctcg cccctgggcg cgaagctgtt tttgcaggcc ttgaccgaga atttgctgcg
     1141 ctacgagaaa aactttggcg aaatcaaaat ccccagagga cactcgctgg ctgaccagct
     1201 ttttagaaca gcagacccaa aagacccgtc ggaagaaggc tagtttgaag aatttagaaa
     1261 aaatctgtga gcaaattcac gaggaattca acgcgaaaac cgatcagcgt gagcaagctc
     1321 tcaaaaacgc gcgcgagttg attcgccgtg cctcgcttac cattcgcgct attcacaggg
     1381 gcgatgtgca gcaggcacaa gaagaattgc aacagggcca agtgatagtg gatgacattc
     1441 ttcagcatct caagcgtgaa catcctgacc tttattacac aggctacact caggacgcgc
     1501 taaaggagtt ttgcgaagcc aagctaaccg cggcgattat ttacgaccag cctattcctt
     1561 ctcccaacga aatgggcata gaccttcccg cgtggctgaa tgggctgact gaaacaatcg
     1621 gtgagatgcg gcgacgttgc ctggatatct tgcgcacagg ttataccgat gaagttgaac
     1681 gtttgctttt caacatggat gagattttta cccaactggt aacgatggat tatccagatg
     1741 ctgttaccga tggactgcgc cggcggacgg acctggcacg gggtatcatt gagcgcaccc
     1801 gcgcggatat caccaccgcc tatcgtcagg aaatgctgac caagaaaata gatgaattca
     1861 ccgaactgtt agctgaccac gaaactaatc agtaataaaa gcttatcaat agattaagat
     1921 ttaatagaaa agcccgcgca agcgggtttt tctatggctg tattctttgc gcgagaaaaa
     1981 cgccttgatt gattat
//
LOCUS       DAOSFC010000255         1989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_57112_length_1989_cov_5.853671, whole genome shotgun sequence.
ACCESSION   DAOSFC010000255 DAOSFC010000000
VERSION     DAOSFC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57112_length_1989_cov_5.853671"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PK791_06200"
     CDS             complement(<1..304)
                     /locus_tag="PK791_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92938.1"
                     /translation="MGFYHGLSAEAYDRQYTNKALIKRIVDYFKPHKAKLIFVAIVVI
                     LQGFTGSMPYLLVSKVLDEGAAVRPDDRILAILVSAVILIEILGYVFFHIQQRLMAR"
     gene            complement(304..>1989)
                     /locus_tag="PK791_06205"
     CDS             complement(304..>1989)
                     /locus_tag="PK791_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92939.1"
                     /translation="VRHHWFIFLVAAIGAIGNATLAGVPAVQFGKVFEALTTGVGGKQ
                     AFLQGAAIVAISQITRGFLQFQRNFGFEVTAQRIERDTRDEFYTSLLGKSMTFHALQS
                     VGDLMARASNDIREVNYIFSPGINMVFGSMNFLFIPLIIGGRIHPALLITPVLFIIAY
                     FLAVSHYLKVLQPVTSKVRSSFGVMNSRIAEDMDGIETVKAAAQEQAEIERFRKNIST
                     YRDASVKQGDIEARFIPMLLLALAMGFGLLHALYLARLGEITLANVITYYSALSMLGF
                     PTNTSVMAYSRISLGLASARRLLAIMNTENNLDQNEDGYAQRIKGDVSFRNVSFSYDG
                     KVNNLKDISLEAKAGQTIAVVGQTGSGKTSLIRLINRTYDPQEGHIFIDGVNLRDWRL
                     DTLRSQISIIEQDIFLFSKSIAENIAFGKPGATQEEIEEAARKAQAADFIAELPDGYD
                     TVIGERGATLSGGQRQRIALARAFLTDPRILILDDSTSAIDSQTEDEIQKAIKTASEG
                     RTTFIVTHRLSQIRWADLIIVLKQGKISEIGTHYELMERSSAYRRIFSEDKQI"
BASE COUNT          496 a          439 c          539 g          515 t
ORIGIN      
        1 cccgcgccat gagccgttgc tggatatgaa aaaagacata gcctaagatt tcaattagga
       61 taaccgcgct aaccaggata gccagaatgc ggtcatcggg acgtactgcc gcgccttcgt
      121 cgaggacttt tgagacaagc aggtagggca tggaaccggt aaaaccttgc agaatgacca
      181 caatagcgac aaaaataagt ttggctttgt gtggtttaaa atagtccaca atgcgtttga
      241 tgagcgcttt gttggtatat tggcggtcat aagcttccgc tgaaagtccg tgataaaagc
      301 ccattagatt tgcttatcct cagagaaaat tctacggtag gcacttgaac gttccattaa
      361 ttcatagtga gtaccgattt ctgaaatttt gccttgtttt aggacgataa tcaggtctgc
      421 ccagcgaatt tgagaaagcc gatgggtgac gatgaacgtt gtgcgacctt cagaggcggt
      481 tttgattgct ttctggattt catcttcggt ttggctgtcg atggcactgg tggagtcatc
      541 aagaatcaga atgcgtgggt cggtaaggaa cgcgcgtgcc agggcgatgc gctgacgttg
      601 cccgccagaa agtgtcgcgc cacgttcgcc gatgacagta tcgtagccat cgggcagttc
      661 cgcgatgaaa tcagctgcct gggctttgcg cgcggcttct tcaatttctt cctgcgtggc
      721 accgggtttg ccaaacgcga tgttctcagc gatgcttttt gagaagagaa aaatgtcttg
      781 ttcgataatc gatatttgac tgcgtaaagt gtctaaacgc cagtctctga gattcacacc
      841 atcgatgaaa atatgccctt cctgcgggtc gtaggtgcgg ttgatgagac gaatgagact
      901 ggttttaccc gagccggttt gaccaaccac agcgatagtc tgaccagcct tggcttcgag
      961 actgatatct ttaagattat ttacttttcc atcgtatgaa aagcttacat ttctaaatga
     1021 aacatcgcct ttgatgcgtt gggcgtaacc gtcttcgttt tgatcgagat tgttttcggt
     1081 attcatgatg gctaagagac gtcgcgcgct ggcgagtcca agcgagatgc gcgaataggc
     1141 catgactgat gtgttggtag ggaagcccag catacttagc gcgctgtaat aggtgatgac
     1201 gttggcgagt gttatttcgc ccaggcgtgc caggtaaagc gcatgcaaaa gtccgaaacc
     1261 catcgccagt gccagcaaga gcatgggtat aaaacgtgct tcaatatcac cttgttttac
     1321 gctcgcatcg cggtaagttg atatgttttt gcggaagcgt tcgatttccg cttgttcctg
     1381 agcagcggct ttgacggttt caatgccgtc catatcttca gcgatgcgac tgttcatcac
     1441 accaaaactg ctgcgcactt tgctggttac aggttgtaag actttaaggt aatggctgac
     1501 tgccaaaaaa taggcgatga tgaagagaac aggtgtaatc agcaatgcgg gatgaattct
     1561 cccaccgata ataagcggga taaagagaaa attcattgag ccaaagacca tattgatgcc
     1621 ggggctgaag atgtaattga cttcgcggat gtcgttactg gcgcgtgcca tgaggtctcc
     1681 aactgattgt aaagcgtgga aggtcatcga tttgccgagc aggctggtgt agaactcgtc
     1741 gcgtgtgtcg cgttcgatgc gttgggcggt gacttcaaaa ccaaagttac gctgaaattg
     1801 cagaaacccg cgggtgattt gcgagatggc aacgattgcg gcgccttgta aaaaagcttg
     1861 ttttccgcca acaccagtgg tgagcgcttc aaagactttg ccaaactgca cggcgggaac
     1921 accggccagg gtggcgttgc caatcgcgcc aatagctgct accaggaaga taaaccaatg
     1981 atgcctgac
//
LOCUS       DAOSFC010000256         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_57157_length_1988_cov_4.039317, whole genome shotgun sequence.
ACCESSION   DAOSFC010000256 DAOSFC010000000
VERSION     DAOSFC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57157_length_1988_cov_4.039317"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PK791_06210"
     CDS             complement(<1..1219)
                     /locus_tag="PK791_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH92940.1"
                     /translation="MKVIKLLFQKMRQYDPKMLWMLAANAVSSALYPFIWVLVPAIIL
                     RYYKSWELWQLILLLAGAGILAMLFGFIVEWLQGNYRMRMNTVRYNLIHDLTEATLRM
                     PYGNTLKPDMLDRIEFARNTVSNTQQGAGGIMLRFLPLFGEILAVFGFIGVLSSLSPW
                     MLLVIAVLLITHFWGYKTIANVQEKAWQAFAPYWRRVYDMTNISHDPLRKKDILLYNT
                     WEMLKTYILGFMQKFLEMYQSQLKTETKVGCLLAFVDLLRDVVIFGWVIVQFLNGRID
                     AATFVLYTGGLLSFILVMQSLARNLAEIQLESVRFSNYIKLMEEIDTQIAADKASTTA
                     VSETSPDIEIRIEDLSFTYPNAEKPVISALNLTIPAGEKLALVGENGAGKSTLIKLLC
                     RLYRPSSGRILMNG"
     gene            complement(1326..>1988)
                     /locus_tag="PK791_06215"
     CDS             complement(1326..>1988)
                     /locus_tag="PK791_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92941.1"
                     /translation="GGKTWQTVFSHEPGQVSDNLPSSGSKNGFTFLDANVGFITGSEP
                     VVEDLYLYRTADGGQNWEKKSCENLPVFGEADMWMPSPVRRINPNVAYVAIEALIAER
                     DTTVTHWCKTEDAGESWQYVSSRDQIEFSDFGSPTFGVAYGNGKMLRTIDGGLTWDDY
                     TPNTCPGMVPVSVQVVSDVLAYMVCSSNAPGDLEALNQNRLFVSMSNARLWQTVEAAI
                     IK"
BASE COUNT          548 a          468 c          469 g          503 t
ORIGIN      
        1 tgccattcat cagaatacgt ccggagctgg ggcggtaaag ccgacagaga agtttaatga
       61 gtgtactctt gccagcgccg ttttcaccaa ccaaggctaa cttttcgccc gcgggaatgg
      121 tgagattgag cgcggagatg accggctttt ccgcgttggg gtaagtgaag ctcaagtctt
      181 caatacgaat ttcgatatca gggcttgttt ctgaaaccgc agtggttgat gctttatctg
      241 ccgcaatttg cgtgtcaatt tcttccataa gcttgatgta attagaaaaa cgcacacttt
      301 ccagttgaat ttccgccagg ttccttgcaa gactttgcat gacaaggata aatgagagta
      361 gcccgccggt gtaaagcaca aaggttgcgg catctatcct gccattgaga aactgaacga
      421 ttacccaccc aaagataacg acatcgcgaa gcaaatccac gaaagctaaa agacagccta
      481 ctttagtttc ggttttaagc tgtgattgat acatttcaag aaacttttgc ataaaaccaa
      541 gaatgtatgt tttcagcatc tcccaggtgt tgtaaagcag tatgtctttt tttcgtaaag
      601 ggtcatggct gatgttggtc atatcataaa ctcgccgcca gtaaggtgcg aatgcttgcc
      661 aggccttttc ctgcacatta gctatggttt tgtagcccca aaagtgagtg attaacaata
      721 ccgcgatgac cagaagcatc catggcgaaa gtgagcttaa aaccccgatg aaaccaaata
      781 cagccaaaat ttcaccaaac aagggtaaaa agcgcaacat aatgccgcca gcaccttgct
      841 gagtattcga aaccgtgttg cgcgcaaact caattcggtc aagcatatcc ggtttgagcg
      901 tgtttccgta aggcatgcgc agagttgctt cggtgaggtc atgaatcagg ttatagcgta
      961 cggtattcat gcgcatacgg taattgcctt gtagccactc gacgataaag ccaaagagca
     1021 tagccaggat gcctgcgcca gcaagtaaaa ggatgagttg ccaaagttcc cagcttttgt
     1081 agtaacgcaa aataatagct ggcaccagaa cccatataaa gggatacagc gcggaactga
     1141 ccgcgttggc tgccaacatc cacagcatct tggggtcata ctgacgcatt ttttgaaaga
     1201 gcagtttgat gactttcatc gtatccactt tcgcctgacg acccttagta tattatagaa
     1261 atactgttcc tggataaaca aaacagccca ggaatgtgcc tgggctggga actaaagact
     1321 aaaggctact ttatgatggc ggcttcaacg gtttgccaaa gtcgcgcgtt actcatgcta
     1381 acaaaaaggc ggttttggtt gagcgcttca aggtctcctg gcgcgttgga cgaacacacc
     1441 atataggcta aaacgtctga aacaacctga acactaactg gcaccatacc cgggcaagtg
     1501 tttggcgtgt aatcgtccca tgtcaagcca ccgtcaattg tgcgtaacat ttttccattg
     1561 ccataggcga cgccaaaggt gggcgaacca aagtcgctaa actcaatttg gtcacggctg
     1621 gaaacatact gccagctttc accggcatct tcggttttgc accagtgggt gacggttgta
     1681 tcgcgttcgg caataagcgc ttcaatggcg acataagcca cgttaggatt aatgcgtctt
     1741 actggtgaag gcatccacat atccgcttcg ccaaaaacgg gtaaattctc acagcttttc
     1801 ttttcccagt tttgaccgcc gtccgcggtt cgataaagat agagatcttc aaccacaggt
     1861 tcgctgccag tgatgaagcc aacattagca tccaaaaaag tgaagccatt cttgctgcct
     1921 gaacttggta aattgtcaga aacctgtccg ggttcgtggc tgaagacggt ttgccaggtt
     1981 ttgccacc
//
LOCUS       DAOSFC010000257         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_57652_length_1977_cov_3.749220, whole genome shotgun sequence.
ACCESSION   DAOSFC010000257 DAOSFC010000000
VERSION     DAOSFC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57652_length_1977_cov_3.749220"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..409)
                     /locus_tag="PK791_06220"
     CDS             complement(53..409)
                     /locus_tag="PK791_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOH92942.1"
                     /translation="MEEKNFICTACPMGCKLIVRLDGNEVVQVLGHRCRRGENYGAQE
                     ATTPKRMLATTVRARGGLHPLLPVYTSAEVPKAKMQEILAILREVDITCPVKMGDVIL
                     KDIADTGVDILASRDL"
     gene            complement(411..1691)
                     /locus_tag="PK791_06225"
                     /pseudo
     CDS             complement(411..1691)
                     /locus_tag="PK791_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
     gene            complement(1681..>1977)
                     /locus_tag="PK791_06230"
     CDS             complement(1681..>1977)
                     /locus_tag="PK791_06230"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOH92943.1"
                     /translation="LVKLDPAYARVICRCENITEAEIVREIHAPIPATTYDAIKRRTW
                     LGTGRCQGGFDMPLVTQILARELGISELEVSKRGDGSRFLFRRSKDIQESDHEA"
BASE COUNT          430 a          557 c          540 g          450 t
ORIGIN      
        1 catcgtgggg agtgtttacg actgcgtaca tcgcgaatga gcctgcccgc gcttataagt
       61 cccgactggc gaggatatca actcctgtgt cagcaatatc cttcaatatc acatcgccca
      121 tttttaccgg gcaggtgata tccacctcgc gcaaaatcgc caggatttcc tgcattttgg
      181 ctttgggcac ttcagccgaa gtgtaaaccg gcaaaagcgg gtgcaagcct ccacgcgcac
      241 ggacggttgt ggcgagcatg cgtttgggcg tagtggcttc ctgggcgcca tagttttcac
      301 cccgccggca acggtggccc aaaacctgaa cgacttcgtt gccatcaagg cgcacgatga
      361 gtttgcaacc catcgggcac gcggtgcaga taaaattttt ctcttccatg ctaggcctcc
      421 gatttcgcgt aaatatccag gaaaagttcg cccgctttca ggacgtcatc aaacatcgac
      481 ggggtcagct tgacgctcac catctcgccg ggccgggcgt aacggtctga gcggcgcacg
      541 atggactggc tctcgctgcg cacttcaaac caaacgtctt cttcaatagg ttgcgtcacg
      601 cggcactgca aaacgacagc ctcgtccttt agggcttgtg ggtctaaaaa ctggggcacg
      661 acgtagcgca cattctttcc cgcgttgact ttgatgcttt tctgtggttt tgccagcgtg
      721 ccattcgcga acagtgccgc gccgtgaccg gcttgattgc cagcttcgga aacataatca
      781 accaaatcat aaacatgcac cacattgccg gccgcgaaga tgccggggaa gttagtctga
      841 aaattttggt caacgactgg cccgccagtg accgcgctta ataagatacc agcttttttg
      901 cttagttcat tctcagggat gagaccaacg gagagtaaaa gcgtgtcgca ggggatgatt
      961 cgttccgtac caggaacaaa ttgccatttt tcgtccacgg cgacagcttc caccgcttca
     1021 acacggtttt tgccgatgac gcgtttgacg gtatggcgca ggtgcaaggg cacgttccag
     1081 tcttccagac attgcacata gttgcgcatg agaccggaga gataacttta tacttccaaa
     1141 acgctttcaa ccctggcgcc ttcaagcgtc aggcgacgcg ccataatcat accgatatcg
     1201 ccagaaccca ggatgacaaa gcgttttcca ggcataaagc cctgtacgtt cacccagcgt
     1261 tgggcggtgc cggcggtaaa aacaccggca ggtcgcgtgc cgggaatgcg gatttgcggg
     1321 cgggttcttt cccggcagcc catcgccaaa acgatcgctt tggcgtcgat gctaaagtaa
     1381 cccttgcgcg cgctggaaag ccataacagg cgttcggggg tgatttccat caccatggta
     1441 tccagcataa agtcgatttc aagcgcgcgg gcttcgtcga taaagcgttg cgcgtaggcg
     1501 gggccaggca agtcttcctt gaaggtgtgt aagccaaatc cgttatgaat acactgtagc
     1561 aaaatgccac ctggttcgcg gtcgcgttcg atgaccaaaa ccttctccgc gccggctttt
     1621 ttagcctcaa tggcgctagc cagccccgcg ggaccggctc cgatgacgac gacgtcataa
     1681 ttatgcttca tggtcgctct cctggatgtc tttgctacgc cggaagagaa agcgtgagcc
     1741 atcgccacgt ttgctgactt ccagttccga tatgcccagt tcgcgtgcca aaatctgggt
     1801 gaccaggggc atatcgaacc caccctggca acgtccggtg cccagccagg tgcggcgctt
     1861 gatggcgtcg taggtcgtgg cggggatggg cgcgtggatt tcgcgcacga tttctgcttc
     1921 ggtgatgttt tcgcagcgac aaataacccg cgcgtaagcc ggatcaagct tgaccag
//
LOCUS       DAOSFC010000258         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_58500_length_1958_cov_6.400946, whole genome shotgun sequence.
ACCESSION   DAOSFC010000258 DAOSFC010000000
VERSION     DAOSFC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58500_length_1958_cov_6.400946"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PK791_06235"
     CDS             complement(<1..837)
                     /locus_tag="PK791_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92944.1"
                     /translation="MNTEQNLSNENPAQEPLPQAYPAYDGLPPSPPPPPQPKPKDVVP
                     VKHSWLLFLMAFVLALGIDLLIFEQSSGKQFAIIVNAILLGAVVLSLAEKRKIPWQSW
                     PLMAMISLSSLLTVFRVEPFTVAALIIFVVFALPLLLMSMFSGQWAVYRIREYFKRYF
                     FITAHGVIGFPRAIVEGVRTQRNPGQRKKVFSIIGAIILGVIIAIPILLLFGKLLSSA
                     DSTFAKNIEAFLNFFKFDSVEAFWPQFFIVLFFFWGLAGLLFYMLSRSHRVEPVEPDK
                     ALL"
     gene            1078..1932
                     /locus_tag="PK791_06240"
     CDS             1078..1932
                     /locus_tag="PK791_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH92945.1"
                     /translation="MKKVKIFTDSTAYIPQEYVDKYDIGIIPLTLNWLGKSYLDGVEI
                     GATEFYTQLAASTELATTSAVPMGQFIEAFKPYLDEGREVFYLGFTSGLSTTVEFAFQ
                     AQKAMGNPENLRIMDTKIISMALTLMVLEVCKKAEQGASLDELEAFAKDLYPRIGVYF
                     TVDDLIYLHRGGRINTAKRLLGSVLNLKPILMIRDGKIELVESVRSKKKALKRMIDLI
                     ERDIDGRTPVKIGPFHALAYDEMIEMEEEAIERLKPVESIRSEVSPVIGSHVGPGTVS
                     MAYLVGEE"
BASE COUNT          560 a          460 c          457 g          481 t
ORIGIN      
        1 cagcagggct ttatcgggct cgactggctc aacacgatgc gaacggctaa gcatataaaa
       61 caaaagaccc gccagtcccc agaaaaagaa cagcacgata aaaaactggg gccaaaaagc
      121 ttctactgag tcaaacttga aaaagttcag gaaggcttca atgttcttgg caaaggtgct
      181 atccgctgat gacagcaatt ttccaaaaag caacaaaatc ggaatggcaa taatcacgcc
      241 caaaataatc gcgcctatga tgctaaagac ttttttgcgt tgaccagggt ttctttgcgt
      301 tcgcacgcct tccacgattg ccctcgggaa gccgattacc ccatgcgcag taataaagaa
      361 gtagcgctta aagtattcac gaatgcgata tactgcccat tgtccgctaa acatgctcat
      421 cagcaacaag ggcaaagcaa atacgacaaa gattatcagt gccgcaacgg taaagggctc
      481 aacacggaat acggttaata gtgaagaaag cgaaatcatc gccatgagtg gccaggattg
      541 ccaggggatt ttgcgtttct ctgccaggct caaaacgacc gcgcccaaca atatggcgtt
      601 cacgatgatg gcaaactgct tgccggaaga ctgttcaaag atgagcaggt cgattcccag
      661 tgctaaaaca aatgccatca gaaacaacag ccagctgtgt ttgacaggca ccacatcttt
      721 tggttttggc tggggtggag gtggcgggct tgggggtaag ccatcataag ccgggtaggc
      781 ttgtggcagc ggctcttgcg ccgggttttc gtttgataga ttttgttcag tattcatttc
      841 aaaaaacctt tcacatttta ttatatcgct atccatgata agcaatttcg cgtctcaatt
      901 actgtcaaaa gctatcaacg ctctctaaat tcctcgcagg tggcccacgc gtcagtcagg
      961 ttaaatgctt cagaataaaa gatatgacat aagtaacctt ttaaacgtgt ttattttcac
     1021 tcagttgacc gctcaactat gctatactgt atgtatcaag taatggaagg tttattcatg
     1081 aaaaaagtca aaatttttac cgattcaacc gcatacattc cccaggaata tgttgataaa
     1141 tacgacatcg gtattattcc actaaccttg aactggttag gaaaatccta tttggatggc
     1201 gttgagattg gcgccaccga gttttacacc caactggcag ccagcaccga actggctacc
     1261 accagcgcgg tcccgatggg gcaatttatc gaagctttca aaccctatct agatgaaggc
     1321 cgcgaagttt tctatttggg tttcacctca ggactaagca ccactgtgga atttgcattc
     1381 caggcccaaa aagcgatggg caatcctgaa aacctgcgta ttatggatac gaaaattatt
     1441 tccatggcac tcactctgat ggtgctcgaa gtctgcaaaa aagctgaaca gggcgccagt
     1501 ctggacgaac tcgaagcttt cgccaaagac ctgtatccac gaatcggtgt ttatttcacc
     1561 gttgatgacc ttatctatct ccaccggggt ggccgcatca acaccgccaa gcgccttttg
     1621 ggctctgtgc tcaatctgaa gccgattttg atgattcgtg atggcaagat tgaactggtt
     1681 gaaagtgtcc gctcgaaaaa gaaagccctg aaacgcatga ttgacctgat cgaacgggat
     1741 attgacggac gcacgccggt aaagattggc ccgttccatg ccctggccta cgacgaaatg
     1801 attgaaatgg aagaagaagc cattgagcgc ctcaagcctg ttgagtcgat tcgctccgaa
     1861 gtcagccccg tgattggcag tcacgttggc cctggtacgg taagcatggc atatctggtt
     1921 ggcgaagaat agagtttttc gccaagtcag acttaaga
//
LOCUS       DAOSFC010000259         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_58554_length_1957_cov_3.861199, whole genome shotgun sequence.
ACCESSION   DAOSFC010000259 DAOSFC010000000
VERSION     DAOSFC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58554_length_1957_cov_3.861199"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PK791_06245"
     CDS             <1..638
                     /locus_tag="PK791_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOH92946.1"
                     /translation="LVEASENEEIKAAVDEHKRLQDEVFPDLSVGLVHGRLKPAEKDE
                     VMLKFRDREFDILVSTSVVEVGVDIPNATMMVIEGANRFGLAQLHQFRGRVGRGEAQS
                     YCFLIPQTADDAENERLNIMTRTNNGFELAEYDLRLRGPGDFLGTKQAGYLDLKMANL
                     FDVQLIKKARDMAEWLLASDPDLEEEDNADLKQVLDHFWASQAEDGSGDLS"
     gene            638..1129
                     /gene="coaD"
                     /locus_tag="PK791_06250"
     CDS             638..1129
                     /gene="coaD"
                     /locus_tag="PK791_06250"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOH92947.1"
                     /translation="MTKVVFPGSFDPIHYGHIDIATRAAKLFDEVVIAVYDRPLKSLF
                     LNPATRITLTKEALAHLENVSVMGYSGLTVDFAESIGATAIVRGLRVFSDFELEFRMT
                     LANRRLKPKIDTIALITTEENTFLSSSTVKEIASLGGDVSSMVPEHVNRALIARYREI
                     EAE"
     gene            1232..1681
                     /locus_tag="PK791_06255"
     CDS             1232..1681
                     /locus_tag="PK791_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92948.1"
                     /translation="MDILHLVDRLEEIVNTGKSVPLTRNVIVDTTALMDIIDQMRISI
                     PEEIKKAQQITAQKDRILAQAQEEANRTVALAREKSEKLIEKSEVYQAAQAKVDMITD
                     KASKEASQSMMDADRYVIDTLSALEQELNKVLAQVRNGIAALSPDDK"
BASE COUNT          518 a          420 c          507 g          512 t
ORIGIN      
        1 ccctggtcga agcttcggaa aatgaagaaa tcaaagccgc ggtggacgaa cataaacgct
       61 tgcaggatga agtttttccg gatttgagtg tcggtttggt gcatggacgt ttgaaaccgg
      121 ctgaaaaaga tgaggtgatg ctgaaattcc gcgaccgcga atttgacatc ctggtttcaa
      181 cttctgttgt tgaagtaggt gtggatattc ccaacgccac gatgatggtg attgaggggg
      241 ctaatcgttt tggcttggcg caactgcatc agttcagggg cagagtgggg cgtggtgagg
      301 cgcagtccta ttgttttctt atcccgcaaa cggctgatga tgccgagaat gaacgtctga
      361 acatcatgac ccgcaccaac aatggctttg aattagcgga atacgaccta agactgcgtg
      421 ggccgggtga ctttttgggt accaagcaag ctggttacct ggacctcaaa atggcgaatt
      481 tgtttgacgt gcaactgatt aaaaaggcac gcgatatggc agagtggctg cttgccagcg
      541 accctgattt ggaagaagaa gataatgccg atcttaagca ggtgctggat catttttggg
      601 catctcaggc tgaagatggc agtggagatt taagctgatg acaaaagttg tttttccggg
      661 aagttttgac cccattcact atgggcatat tgatatcgcc acacgggctg ccaagttgtt
      721 cgacgaagtt gtgatcgcgg tttatgaccg acctctcaaa agcctgtttc ttaaccctgc
      781 tacacgtatc actcttacca aggaagcttt ggcgcatctc gaaaatgtga gtgtgatggg
      841 ctattctggt ttgacggttg atttcgccga atcaatcggc gcgacagcga ttgtgcgtgg
      901 tctgcgcgtg ttttctgatt ttgaacttga atttcgcatg accctggcca accgcaggct
      961 gaaacctaaa attgacacca tcgcgttgat aaccaccgaa gagaacacct ttttatcgtc
     1021 cagcacggtc aaggagattg cctcgctggg cggcgatgtg agcagtatgg tgccggagca
     1081 tgtcaaccgc gctttaatcg cgcggtaccg cgaaattgaa gcagaataag cataaaactt
     1141 tacaaattgc ttttcaaacg acctgaatat ttaataatta atgcgaatag tgataaacta
     1201 aatcaacacc gataatcgtg atggaggaga tatggatatt ttacatttgg ttgaccgcct
     1261 ggaagaaatc gttaataccg gcaaatccgt tccgttaaca cgcaatgtga ttgttgatac
     1321 cactgcttta atggatatta tcgatcagat gcgcatttct attcctgaag aaatcaaaaa
     1381 agcccaacaa attaccgcgc aaaaggaccg cattttagcc caggcgcagg aagaagccaa
     1441 ccgcacggtg gcactggcac gcgaaaaaag cgagaaactt atcgagaaat cagaagtcta
     1501 ccaggccgcg caagccaagg tggatatgat tactgacaag gcaagcaaag aagcctcgca
     1561 aagcatgatg gacgccgacc gctatgtgat tgatacgctt tccgctctcg aacaggaact
     1621 gaacaaagtg ctggcacagg tgcgcaacgg cattgccgcc ctctcgccgg acgataaata
     1681 aaattatcaa aatacttatt ttgacgtgac attttagggc acgtctgtat tatcttgaaa
     1741 taaaaaagca cagtatggtc gctgtgcttt tttgatgact cgtattaaga ccttagttat
     1801 gccacggttt gggtgtcgct gctttgttgg gttctttgcg ccattttgtt tttcgctgga
     1861 cgggggtggc ggggtggggt ttcttttggc gatttactat atatcgcctc accagccagg
     1921 gcttctttca agacttcgtc catgtgttca acgaaca
//
LOCUS       DAOSFC010000260         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_60335_length_1919_cov_4.028970, whole genome shotgun sequence.
ACCESSION   DAOSFC010000260 DAOSFC010000000
VERSION     DAOSFC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60335_length_1919_cov_4.028970"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PK791_06260"
     CDS             complement(<1..295)
                     /locus_tag="PK791_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92949.1"
                     /translation="MTQFVEIPDTSLVLVAIIPNKRDLDIARMLGWYRIPLKSAPKII
                     SVDYIAFYQTAAFNERERWQIRWMAPIQGHELTTRLDLFRDEADHPRANKNTSN"
     gene            complement(338..1276)
                     /locus_tag="PK791_06265"
     CDS             complement(338..1276)
                     /locus_tag="PK791_06265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH92950.1"
                     /translation="MTKPKYTVIAPIYNEIENIPHLYARVCEVMDQTGEAWELILVDD
                     GSKDGSTDALRELGKKDDRVRPVIFARNFGHQIAVSAGMDYARGDAIAIIDADLQDPP
                     EVILELIEKWKEGYDVVYAVRSEREGESWFKLTTAALFYKFIQKITDVDIPLNTGDFR
                     LLDRKVLNVMNSMPERNRFLRGMSSWAGFKQIGVEYKRHARFAGETHYPLKNMIKLAL
                     NAITGFSYWPLQMLSKTGVGLVVLSLLLLVLFIILALVGKVDLTFVLATWLTVIFLAG
                     VLLIGMGILGEYMGRIYDEAKGRPLYIVAEAPDKDE"
     gene            1447..>1919
                     /gene="sdaAB"
                     /locus_tag="PK791_06270"
     CDS             1447..>1919
                     /gene="sdaAB"
                     /locus_tag="PK791_06270"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HOH92951.1"
                     /translation="MSNNSLFDVIGPIMVGPSSSHTAGAAKLGRLARQLVHGDIVEVR
                     FTLYDSFARTYRGHGTGKALLGGVMGYDLDSEELKHAETIATFRGLKFSFEEGHKDGI
                     HPNSVDIHLTEDNGKKTSLSGSSIGGGNVVITSINGIETRLQTNLPAIVTHHRDRV"
BASE COUNT          468 a          445 c          513 g          493 t
ORIGIN      
        1 aatttgaagt attcttgttg gcgcgggggt ggtcggcttc atcacggaac aaatccagtc
       61 gtgtggtaag ttcgtgcccc tgaataggtg ccatccaacg gatttgccag cgttcgcgtt
      121 cgttgaaagc ggcggtttga taaaaggcga tgtaatcaac agagatgatt tttggtgctg
      181 attttagggg gatacgatac cagcccagca tgcgcgcgat gtcgagatcg cgtttgttgg
      241 ggattattgc caccagaacc aggctggtat cgggaatttc gacaaactgt gtcatttaat
      301 caaaagttat cgctttgata tctgcaaaac ctaaggttta ttcgtcttta tcgggtgctt
      361 ctgccacgat gtaaagcggg cgtcctttgg cttcgtcgta aatgcgaccc atgtattcgc
      421 ctaaaatgcc catgccgata agcaagacgc cagccaggaa aatgacggtt agccatgtgg
      481 cgagcacgaa ggtcaaatcc accttaccaa ccagggcaag aatgatgaac agcaccagca
      541 acaataagct gagaactacc agtccgacac ctgttttgct gagcatttgc aagggccagt
      601 aggagaagcc cgtaatggcg ttgagggcca gttttatcat gtttttgagg ggataatggg
      661 tttcgccggc gaagcgcgca tggcgtttgt actccacgcc gatttgctta aagcccgccc
      721 aggaactcat gccacgcaaa aagcgattgc gttcgggcat gctgttcatc acattcagga
      781 ctttgcggtc gagcaagcgg aaatcaccgg tgttgagcgg gatatccacg tcagtaattt
      841 tttggataaa tttataaaac aacgcggcgg tggtgagttt gaaccagctt tcgccttcgc
      901 gttccgaacg cacggcgtag accacgtcgt agccttcttt ccatttctcg atgagttcca
      961 ggatgacttc cggcgggtcc tgcaaatccg cgtcgataat ggcgatggcg tcaccacggg
     1021 cgtaatccat gccggcactc accgcgattt ggtggccaaa attgcgcgcg aagatgaccg
     1081 gtcgcacacg gtcatccttt ttgccgagtt cgcgtaaggc atcggttgaa ccgtctttac
     1141 tgccatcgtc caccaggatc agttcccagg cttcgccggt ttggtccatc acttcgcaaa
     1201 cgcgggcgta aaggtgggga atgttttcaa tttcgttgta aatgggcgcg ataactgtgt
     1261 atttgggttt tgtcacgtat tatctccttt aggttcttta tcgttgcttt tttcagagta
     1321 actttctaaa gtgcatctca attctacttc attgagagaa taagtggaaa taacacgatc
     1381 gtaaaacggt agcgcggata tccgtcatga tacaattacg gcagtaattc aaaacgaggc
     1441 aataagatga gcaacaacag tttgttcgac gttatcggtc ctattatggt tgggccatcc
     1501 agttcccaca ccgcgggggc tgcaaaactg ggcaggttag cccggcagct cgtgcacggc
     1561 gacattgttg aggtgcgttt cacattgtac gactcttttg cacgaactta tcgtgggcat
     1621 ggcacgggca aggcgctttt gggcggtgtg atgggatatg atctggacag tgaagaactc
     1681 aagcacgccg aaacaatagc aacctttcgc ggtttaaaat tcagttttga agaaggccac
     1741 aaggacggta ttcatcctaa ctcggttgat atccatttga ctgaagacaa tggcaagaaa
     1801 accagtctga gtggttcatc aatcggtggg ggcaacgtgg tgatcacgtc gattaatggc
     1861 atcgagacca ggctgcaaac gaatttaccc gcgattgtta cccatcaccg cgaccgggt
//
LOCUS       DAOSFC010000261         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_60883_length_1907_cov_3.960583, whole genome shotgun sequence.
ACCESSION   DAOSFC010000261 DAOSFC010000000
VERSION     DAOSFC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60883_length_1907_cov_3.960583"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1907)
                     /locus_tag="PK791_06275"
     CDS             complement(<1..>1907)
                     /locus_tag="PK791_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH92952.1"
                     /translation="MSLVGMNTLANTLRPALLVHGREILGGDFGVYKSGDVSFSEENR
                     QGFEKLQAEGKIEGFTMIAGSKNLIFKLENDPVWHYAGNALGVDTKTYPLAGNLDIAD
                     KTVKEPIKLLEQENVILITADIAESFAIKTGDTLLISNINVGKPLKVTVAGIIIDTPN
                     HEGDKIYYSYDLARALENGNLQLDTTMVNVSQEKKQALIDYADEIDCWVWSAHSLDTS
                     RQERDSTVRLMLNSAGGLGLLVGGIGVANTMVVLISRRRKQIATWKLLGYENKHIRAI
                     FLLEALMMGAIGSLIGAGLGVLLSKGLTKIFLGTATWLVVWKPDYVSVAIGLGAGMIT
                     SLLFTLWAILRASNVSPASVIRAEDGKAQKGSLVKDIVLLTLIFGVFMVMVTLIIGSV
                     VEGLKYMGIAVLALILVCLFIAAAIWVLTALMPTKRFPQLFLARQHLRRGGITPIMAG
                     AALFVGVLTIFFAALALGSSQNFNVDIAEVWEGPNAVILAPYSEEATVREAVAKLTPI
                     EADMGYRVSLKSVESEYDHFVNPVIFGRESELGFSLEDSATGEIFKSSTGAWVISEGA
                     YMSPEDVAAHKERMAIEKAGLSKDLTVEFADGKVVEIPFAGEYLPAESLPTTLNYFRP
                     IYMQSDALKAI"
BASE COUNT          477 a          527 c          462 g          441 t
ORIGIN      
        1 taatcgcttt cagcgcgtcg gattgcatat aaatggggcg gaagtagttt agggtggtcg
       61 gtaaactttc agcaggtaaa tattcgcccg cgaaaggaat ttcaactacc ttgccatcag
      121 caaattcaac cgtcagatct ttgcttaggc ctgctttttc aattgccatt ctttctttat
      181 gcgcggcaac atcttctggc gacatataag caccttcgct gataacccag gcgcctgtcg
      241 agcttttgaa tatttcgcca gtggcggagt cttccagtga aaagcccaat tcactttccc
      301 gtccaaaaat aacgggattg acaaaatggt catattcgct ttcgaccgac ttcagcgaaa
      361 cccggtaacc catatccgcc tcaataggcg tgagcttggc gacggcttcg cgcacagtgg
      421 cttcttcgga ataaggtgcc aaaataaccg cgttgggacc ttcccagact tcagcaatat
      481 caacgttgaa attttgcgaa cttcccagcg ccaaagcggc aaaaaagatg gtcagcacgc
      541 ccacaaaaag cgcggcaccc gccatgatgg gcgtaatgcc accacggcgc aaatgctggc
      601 gcgccaaaaa gagctgcgga aagcgtttgg tgggcatcag cgcggtaagg acccaaatgg
      661 cagccgcgat gaacagacac accagtatca atgccagcac agcgatgccc atatacttga
      721 gaccttccac aacagagcca atgataagcg tcaccatcac cataaacacg ccaaagatta
      781 gcgtgagcag aacgatgtct ttgaccagcg agcctttttg cgctttgcca tcttccgcgc
      841 ggataacgct ggcggggctc acgttactgg cgcgcaaaat cgcccacagg gtgaacagca
      901 agctggtaat catgcccgcg cccaggccaa tcgccacgga tacgtagtcg ggtttccaga
      961 caaccagcca ggtggcagtg cccaggaaga ttttggtgag cccctttgaa agcaaaacac
     1021 ccagaccggc gccaatgagc gagccaatcg cgcccatcat cagcgcttca agcaggaaaa
     1081 tggcgcggat gtgtttgttt tcgtagccca ggagcttcca ggtggcaatt tgtttgcgcc
     1141 ggcggctgat gagcacaacc atcgtgtttg ccacgccgat accacccacc agcaagccca
     1201 gtcctcctgc cgagttgagc atcaggcgca cggtgctgtc gcgttcctgg cgcgaagtgt
     1261 ccaggctatg cgctgaccaa acccagcaat caatttcatc ggcatagtcg attaaagctt
     1321 gtttcttttc ctggctgaca ttaaccatcg ttgtgtccag ctgtagattg ccattctcca
     1381 gcgcgcgggc aaggtcgtag gaatagtaaa ttttgtcacc ttcgtggttg ggcgtgtcga
     1441 taataatgcc cgccaccgtg actttcagcg gtttgccaac gttgatattg ctgattaaaa
     1501 gcgtgtcgcc ggttttgata gcaaaactct cggcgatatc cgcggtgatt aagatgacat
     1561 tttcctgttc aagaagctta atcggctctt ttaccgtctt atccgcgata tcaagattgc
     1621 ctgccaaagg gtaggttttg gtgtccacac ccaaagcgtt acccgcgtag tgccacaccg
     1681 ggtcgttttc aagcttaaaa atcaggtttt tggaacccgc gatcatggta aagccttcaa
     1741 ttttgccctc agcctggagc ttttcaaaac cctgccggtt ttcttcgcta aaactgacat
     1801 caccactctt ataaacgcca aaatcaccgc ccaaaatttc gcgcccgtga acaagcaaag
     1861 ccggacgcaa agtgtttgcc agcgtgttca tgccgaccaa agacatc
//
LOCUS       DAOSFC010000262         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_61505_length_1894_cov_5.331158, whole genome shotgun sequence.
ACCESSION   DAOSFC010000262 DAOSFC010000000
VERSION     DAOSFC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61505_length_1894_cov_5.331158"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK791_06280"
     CDS             complement(<1..369)
                     /locus_tag="PK791_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOH92953.1"
                     /translation="MKNIINKEIMDWKNKLRNAGYRITDAREQMMDLLINTQTALSPM
                     QIHKALAEQGSQMGLVSVYRNLAVLQENDLVCMVFAPDGSAAYTAGGIGHHHHVLCQR
                     CLNSASFEGCEGFADLIQEIE"
     gene            complement(552..896)
                     /locus_tag="PK791_06285"
     CDS             complement(552..896)
                     /locus_tag="PK791_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92954.1"
                     /translation="MTEQPKQTNWYLLTGIILGLLIGLILSTMIFPAVNRYATPAQLD
                     AIGKDIYREEIALAYAATGSLNRAIDRLMRLEQAQPASILIEQAQRLQAAGGSQQVID
                     ALVLLAERLSGE"
     gene            complement(889..1266)
                     /locus_tag="PK791_06290"
     CDS             complement(889..1266)
                     /locus_tag="PK791_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92955.1"
                     /translation="MKRNRSRNFIITIIVGILLGLALGWLVIKPKAPQNVNITRLRPD
                     FQADAVLMVAESFAVDGDQMLALDRLARLDHGDLLTFVGKALQNAQAYGYAGEDLAQM
                     QKLLESLDLVVYENWKILRGRND"
     gene            complement(1332..1862)
                     /locus_tag="PK791_06295"
     CDS             complement(1332..1862)
                     /locus_tag="PK791_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOH92956.1"
                     /translation="MKKVLVFYKSKTGFTERYARWIAEEVGADILPIEKVNLETIDSY
                     DILVFGGPLFASRISALDKVQKWMEKRPKKTWVIFATGATPSDDSFVKSIERINFPPD
                     TQKPARFYYFLSGINFEKMGWFNRTLIKLFARMSQKNVKTDSGTAPVPPRDLRSVDLC
                     DPIYIEALLKYLRIKR"
BASE COUNT          509 a          482 c          411 g          492 t
ORIGIN      
        1 ttcgatttcc tgaattaaat cagcaaaacc ctcgcagcct tcaaagctcg cggagttaag
       61 gcatcgctgg cacaatacat ggtgatggtg tcctatcccg cccgccgtat aagcggcgct
      121 gccatcaggc gcgaacacca tacacaccaa gtcgttttcc tgcaagaccg ccaggttgcg
      181 atagacgctt accaaaccca tctgactgcc ttgttcagcc agagccttat ggatctgcat
      241 ggggctaagc gctgtttggg tattgataag caaatccatc atttgctctc tggcatccgt
      301 gatgcgatat ccggcattgc gtaatttatt tttccagtcc atgatttcct tattgataat
      361 atttttcaat aagcattata gtccttcgaa aatggatgtc aagaaaggaa gtgtgtacaa
      421 aagacagggc cgaccttggg tgtgagcaaa aattcaagtg tgatcagaag gtcacaccct
      481 aagttcacac accctaagtt caaatgtggt ctcctgacca cgttttcaat tattgtaata
      541 aaacgaaaaa tctattcacc tgataatcgt tcagctaaaa gcaccagcgc gtcaatgacc
      601 tgttgagacc ccccagccgc ctgaagccgt tgcgcttgtt caatcagtat agacgccggc
      661 tgagcttgtt ccaaacgcat caggcggtca atcgcccggt ttagtgagcc cgtcgctgca
      721 tacgccaaag caatttcttc gcggtagata tctttgccaa tcgcgtctaa ctgagccggt
      781 gtggcatagc gattcacggc ggggaaaatc atcgttgaca ggattaaacc aattaataaa
      841 ccaaggatga taccggtcag taaataccaa ttcgtttgtt tgggttgctc agtcattccg
      901 gcctcgcaat atcttccagt tctcatagac cactaaatca agactttcta ggagcttttg
      961 catttgggcc aggtcttcgc ccgcgtaacc ataagcttgc gcgttttgca gggcctttcc
     1021 cacaaaggtc aacaaatccc catggtccaa gcgtgccagg cggtccagcg ccagcatttg
     1081 gtcaccgtca accgcaaaac tttccgcgac catcaacacg gcatcagctt gaaaatcagg
     1141 gcgcaagcgc gtgatgttaa cattttgtgg cgcttttggt tttatgacca accaacccaa
     1201 tgccaggccc agcaaaatcc cgacaatgat ggtgataatg aaattacgtg aacggtttct
     1261 tttcatctgc attctaagaa tgcaatttta gtgccaggaa ggcaaaaacg ggtagatgca
     1321 aggcaaagtt cttagcgttt tatgcgtaga tacttcagca gtgcttcaat atatatgggg
     1381 tcgcacaaat caactgagcg caagtcgcgg ggcgggactg gcgctgtgcc agaatcagtt
     1441 tttacattct tttgactcat ccgggcaaaa agcttaatca gcgttcgatt aaaccaaccc
     1501 atcttttcga aattaatacc actcaggaaa taataaaacc gggcaggctt ttgcgtgtca
     1561 gggggaaaat taatcctttc gatgcttttc acaaagctat cgtcactggg tgtcgcgccg
     1621 gttgcaaaga tgacccaagt ctttttgggg cgcttttcca tccacttttg cactttgtcc
     1681 aaagccgaaa tccgactggc aaacaaaggc ccaccaaaca cgagaatgtc ataggaatct
     1741 atcgtttcga gattgacttt ttcaatgggc aaaatatccg cgcccacttc ttcagcgatc
     1801 caccgcgcgt aacgctccgt aaaccccgtt ttgctcttgt aaaacaccaa aacctttttc
     1861 atggcacacc tccgcgataa ttttaacgca taaa
//
LOCUS       DAOSFC010000263         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_62550_length_1875_cov_5.686813, whole genome shotgun sequence.
ACCESSION   DAOSFC010000263 DAOSFC010000000
VERSION     DAOSFC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62550_length_1875_cov_5.686813"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PK791_06300"
     CDS             complement(<1..1386)
                     /locus_tag="PK791_06300"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOH92957.1"
                     /translation="MTELPKTYDPKVYEEDIYKHWEEKGYFKPTNDPNLPGHDPNIEP
                     FVIVIPPPNVTGALHLGHPMFVTIEDIMVRYHRMKGVPTLWVPGTDHAGIATQLQVEK
                     LLATEGTSRQELGREAFEERVWEWKEKYGGEISRQLRRLGASCDWERERFTLDEGLSN
                     AVREAFVRLWEKGLIYRGARMINWSPGLQTAVSDLEVEYSEEPGKLYYFKYMLADGSG
                     DYLPVATTRPETIPGDTAVAVHPEDERFAKFVGKKVLVPSLGREIPVVADDYVEKEFG
                     TGALKITPGHDPHDYEIGVRHNLELINIMNNDATLNENAGPYAGMERFKARKAIWADM
                     QADGLVIKEEDYQMNVPRSQRGGEIIEPLISTQWFVKMDGLAEAAAKAVNNGEIQFVP
                     ERYTKVFLNWMNNIQDWCISRQLWWGHRIPVWYCQACGEIIVARETPKQCTGCGSSDL
                     HQDDDVLDTWFS"
BASE COUNT          461 a          488 c          366 g          560 t
ORIGIN      
        1 tgagaaccag gtatcgagta catcatcatc ctggtgtaaa tctgatgacc cacacccagt
       61 acattgtttt ggcgtttcgc gagctacgat tatctcgcca catgcctggc aataccacac
      121 tgggattcga tgcccccacc acagttgccg gctaatgcac caatcctgaa tattgttcat
      181 ccaatttagg aatacttttg tgtaacgttc tggaacaaac tgaatctcac cgttattgac
      241 ggctttagca gcagcttcgg ctaaaccatc cattttcacg aaccattgcg tagaaatgag
      301 cggttcaata atctcgccac cccgttgcga gcgggggaca ttcatttggt aatcttcttc
      361 tttgataacc aaaccatcgg cttgcatgtc agcccatatc gccttacgtg ccttaaacct
      421 ttccattcct gcataaggtc cagcgttttc gttaagcgtg gcatcgttgt tcataatgtt
      481 gataagttcc aaattatgac gcacaccgat ttcatagtca tgcgggtcgt gaccaggggt
      541 aatttttaac gcgcctgtac caaattcttt ttccacataa tcgtcagcca caacaggtat
      601 ctcacgtccc aaacttggca caagaacctt tttgccaaca aatttagcga atctttcatc
      661 ttcaggatga actgctacag cggtatcacc aggaatagtt tccgggcgtg tggttgccac
      721 aggcagataa tcacctgacc catccgctag catatacttg aagtaataaa gcttgccggg
      781 ttcttccgaa tactcgacct caaggtcaga aaccgctgtt tgtaagccag gtgaccagtt
      841 aatcatgcgt gcgccacgat aaatcagccc tttttcccat agtcgcacaa aagcttcgcg
      901 cacggcgttt gaaagccctt catctagcgt aaaacgctct ctttcccagt cacatgacgc
      961 acccaatcgg cgcagttgtc ttgatatttc accgccatat ttttctttcc attcccaaac
     1021 acgctcttca aatgcttccc gacccagttc ctgacggctt gtgccttcgg ttgccagaag
     1081 tttttcaacc tgcaactgtg tggcaattcc agcatggtca gtacctggca cccaaagggt
     1141 tggcacacct ttcatccgat ggtaacgtac cataatgtcc tcaatggtca caaacatggg
     1201 gtgccccaaa tgaagcgcgc cggttacatt aggtggtgga atgacgataa caaatggctc
     1261 aatgtttggg tcatgtccag gcaagtttgg gtcgttggtt ggcttaaaat agcctttttc
     1321 ttcccaatgc ttataaatat cttcttcata aactttaggg tcataagttt ttggcagttc
     1381 tgtcatctta tcctctttca cgcttgtctg taataaattt caaataagcg agttaaatta
     1441 aaaagctttc gtcacttgag gacgaaagcg aagttccgtg gtaccacctc aattcggtct
     1501 attttagacc gcactcaggg tgagcgttat cgggctctcc cggaccgttc tactcccttt
     1561 cggtttcttc ggtcatcaat cctggcgact tcaaacttcc tttcccgtga actttctcag
     1621 ccagtaagtt catctctgtc gggcggataa agctttacta ctccaagcgc atattatagc
     1681 aaatattttt aaaacaagtg tatcgctttt gtatatttgc ttttactttt ccatactgat
     1741 ggtttgttca tcccaatgat gccagccatc aaatttttct tcaacaattt ttcggccttc
     1801 gcctggtggg gtcaaaccca tcaattccat gtccgcgtca atcataatgc gcactaactc
     1861 tttgaattgc acctt
//
LOCUS       DAOSFC010000264         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_62871_length_1869_cov_5.917310, whole genome shotgun sequence.
ACCESSION   DAOSFC010000264 DAOSFC010000000
VERSION     DAOSFC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62871_length_1869_cov_5.917310"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PK791_06305"
     CDS             complement(<1..355)
                     /locus_tag="PK791_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH92958.1"
                     /translation="MQNTLHKSTLENGLQIYLKEIHTAPIVSLWLWYDIGARNEVPGK
                     TGLSHWVEHMQFKGTANMTGQEMDHAIARVGGYWNAFTSQDWTTYLETLPANHLELAL
                     KIEADRMQNSLFDPDD"
     gene            complement(436..924)
                     /locus_tag="PK791_06310"
     CDS             complement(436..924)
                     /locus_tag="PK791_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92959.1"
                     /translation="MTDNVRPVLFDPDNKKKPDYSGFPFYRARLGITFVFLGFIIFLV
                     GLRPDLFGLDRGRSIGLIQIITILIGLGMMTWSGVILLRRLWNGKKKTIVGDFGSRVV
                     ATGFVICSFAALADAFGLGTNPVSNVLLGTLQSYGLMVGIVVVCIGFLMLIKPRNKNK
                     HQ"
     gene            complement(935..>1869)
                     /locus_tag="PK791_06315"
     CDS             complement(935..>1869)
                     /locus_tag="PK791_06315"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOH92960.1"
                     /translation="RVAIVFSTLFAAPARSVPTEKRDSQIYDTLEQAHQIYKQQLLAY
                     HQLSEQSPDYFRLIRTQAELKHHLEKWQNAPKAKTPQLPVGLIPLMEGAEGILSFEEL
                     PMWWELGVRIIGLAWRGNRYSGGTKEPGPLSQAGKELLDAMAEHGFVLDISHMDEEAA
                     LQAIERYPGTIIASHANLARLVEGYQGNRLLSDTLLDALLERNAIIGVVPCNSFLRRD
                     WKESGGKASVSLKLAAEQIDAICQRAGDSKHVGLGTDFDGGIGLQSVPAEIDTIADLP
                     KLSAFLKEMGYSQTAIEDINYRNFLNMIQSVLPA"
BASE COUNT          466 a          421 c          511 g          471 t
ORIGIN      
        1 cgtcatcggg gtcaaacaag ctgttttgca tgcggtcggc ttcaatttta agcgccagtt
       61 caaggtggtt agcggggagt gtttcaagat aagtcgtcca gtcctgactg gtaaaggcgt
      121 tccagtaacc accaacgcgg gcgatggcgt ggtccatttc ctgaccggtc atatttgcgg
      181 tgcccttaaa ctgcatgtgt tcaacccagt ggctgaggcc ggttttgcca gggacttcgt
      241 tgcgcgcgcc gatatcgtac cagagccaaa ggctgacgat gggcgcggtg tgaatttcct
      301 tgagatagat ttgcaagccg ttttccagcg ttgatttatg taaggtgttt tgcataggcg
      361 atagactcat taaatgcttg tgatatcaag ttttgggcaa taatgccggt tttatttctg
      421 aagttgatgt gttttctact gatgcttatt tttattacgg ggtttgatga gcatcaggaa
      481 accgatgcaa acgacaacga taccgaccat caggccataa ctttgcagag tgcccaaaag
      541 gacgttggaa acggggttag tgcccagccc aaaggcatca gccagcgcgg caaagctaca
      601 gatgacaaaa ccagtagcaa cgactctgga accaaaatcg ccgacgatgg ttttcttttt
      661 gccattccaa aggcggcgta acaggataac gccagaccag gtcatcatgc ccagtccaat
      721 caggatggtg ataatttgaa tgagcccaat agaacgtcca cggtcaagtc cgaacaggtc
      781 agggcgcagg cctacaagga aaataataaa gcccaaaaag acgaaggtaa tgccaagtcg
      841 ggctctgtaa aaaggaaagc cggaataatc gggctttttc ttattgtctg ggtcaaaaag
      901 aacgggtcga acgttatcag tcatatttga aatcctaagc gggcaaaacc gattgtatca
      961 tatttaagaa gttacgatag ttgatgtctt caatcgcggt ttgagaatat cccatttctt
     1021 tgagaaaagc gctaagtttg ggcaggtcag cgatggtgtc aatttctgca ggtacggatt
     1081 gcaagccgat acccccatca aagtcagtac caagaccaac gtgtttgctg tcgccagcgc
     1141 gttggcagat ggcgtcaatt tgttcagcgg caagttttaa gctgacagat gccttaccgc
     1201 cactttcttt ccagtcgcgg cgtaaaaagc tattacaggg cacgacaccg atgatggcat
     1261 tacgctcgag aagggcgtcc agcaaagtgt cggaaagtaa gcggttgccc tggtagcctt
     1321 ctaccagacg ggcaaggttg gcatgactgg cgatgattgt gcctgggtag cgttcaatgg
     1381 cttgcagggc ggcttcttcg tccatgtggg agatatccag tacgaagcca tgttcagcca
     1441 tcgcgtccag cagttcttta ccggcttggc ttagcggtcc gggttctttg gttccgcccg
     1501 agtagcgatt gccgcgccag gcaaggccaa tgatgcgcac acccagttcc caccacatgg
     1561 gtagttcttc aaagctgaga ataccttcag caccttccat gagaggaatt aggccgacgg
     1621 gcagttgagg cgttttagct tttggcgcat tttgccattt ctcaagatgg tgcttgagtt
     1681 ctgcctgggt gcggataagg cgaaaatagt ccgggctttg ctcactcaac tggtgatagg
     1741 caagcagctg ctgtttatag atttgatggg cttgctcaag cgtatcgtag atttgtgaat
     1801 cacgtttttc tgtaggcaca ctgcgggcag gcgcagcgaa aagtgttgaa aaaacgatag
     1861 cgactcggg
//
LOCUS       DAOSFC010000265         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_62879_length_1869_cov_3.971885, whole genome shotgun sequence.
ACCESSION   DAOSFC010000265 DAOSFC010000000
VERSION     DAOSFC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62879_length_1869_cov_3.971885"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1846
                     /locus_tag="PK791_06320"
     CDS             191..1846
                     /locus_tag="PK791_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOH92961.1"
                     /translation="MNEDSKNKAQGNSNDIEALLGVLPVDVALAVRQKNDFDNLLEII
                     LDLGRCPSARFVSGEVRLLDRFVEHDDIEYVTSRISEFDADNRAGMERTLHRISAIRN
                     RRGRVIGLTCRVGRAVYGTIDIIEDLVNSGESILILGKPGVGKTTILREAARTLADKK
                     RVIIVDTSNEIGGDGDVPHPAVGSARRMQVPKPSLQHEVMIEAVENHNPEVIVIDEIG
                     RELEALAARTIAERGVQLIGTAHGNALENLLLNPTLSDLVGGIESVTLSDDEARRRGT
                     QKTVLERRSPPTFNILVEIQERNRLNVHRDIASAVDAILRGYPLAPEERYRDEKGKIR
                     MVRASQAKAPTASQSLRAGKNMENSPFQRRTGLAEPQAQAKNRKERYEDFVVPEDPVL
                     NLEDSREKRMFVFGVARNRLLQAIKSMNAPVSVVRDLKDADMFLTQRRYYRDRLQPIV
                     DAETKGLPIFVIRSNSMPQIEQFLADTFGLATPQEKREKDDEALAQVDIAVDLLQAGE
                     PYVDLQPTVAHIRKRQHELAREYGLVSQSFGKDPNRYVRIYRD"
BASE COUNT          483 a          469 c          488 g          429 t
ORIGIN      
        1 aggcgatccc atctttgaaa aatcacccgc cgaaggtggc atcttctctg ccgaatcgca
       61 aaattaatgt tgccgggttg tgcgcattag ctttacctaa aagaactggc tcccaaagat
      121 taatgatgct cctatacatc aggcttttct gtgatgctta attccgccaa atacgataaa
      181 attatgggcg atgaatgaag atagtaaaaa caaagcccaa ggcaattcaa atgatattga
      241 agccttgttg ggtgtcttgc ccgttgatgt ggcgcttgcc gttcggcaaa aaaacgattt
      301 tgacaatctc ctggaaatta tcttagatct cggacgttgt cccagcgccc gttttgtttc
      361 gggtgaagtg cgtttgctgg accgttttgt tgaacacgat gatatcgaat atgtgaccag
      421 tcgcatcagc gagtttgacg ccgacaaccg cgcgggtatg gaacgcaccc tgcatcgcat
      481 ttcggctata cgcaaccgac gcgggcgggt tatcggactc acctgccggg tagggcgcgc
      541 ggtctatggc acgattgata tcatcgaaga cctggttaat tccggcgaga gtattctcat
      601 tttaggcaag ccgggtgtgg gcaagaccac catcctgcga gaagccgccc gaacgctggc
      661 ggataaaaaa cgtgtcatca ttgtggatac atccaacgaa atcggtggcg atggcgacgt
      721 gccccatccc gcggtaggtt ctgcgcgccg gatgcaagtg cctaaaccgt cgcttcagca
      781 tgaagtgatg attgaggcgg ttgaaaacca taaccctgaa gttattgtga ttgacgaaat
      841 cgggcgcgaa cttgaagccc ttgccgcgcg cactattgca gaacgcggtg tgcaactgat
      901 tggcacagcg cacggtaacg cgcttgaaaa cttgcttttg aatccaaccc tttctgactt
      961 ggttggcggc atcgagtcgg ttacgctttc tgatgatgaa gcacgccggc ggggcacgca
     1021 aaaaaccgtg ctggaacgcc gttcaccccc aactttcaat atcctggttg aaattcagga
     1081 gcgcaatcgc ctgaacgtgc accgcgatat cgccagcgcg gtggatgcca tattgcgtgg
     1141 ctatccgctg gcgcctgaag agcgttatcg cgatgagaaa ggcaaaattc gcatggtgcg
     1201 tgccagtcaa gccaaagcgc ctacggcttc ccaaagctta agggctggta aaaacatgga
     1261 aaactcacct ttccaacgcc ggacaggact tgccgagcca caggcacaag caaagaatcg
     1321 caaagaacgc tacgaagact ttgttgtccc agaagaccct gtcttaaatc tggaagacag
     1381 tcgcgaaaaa cgaatgtttg tctttggcgt agcgcgaaac cgcttgttgc aggccattaa
     1441 gagcatgaac gcgccggtaa gtgtggtgcg cgatttgaaa gacgcggaca tgttccttac
     1501 ccagcgtcgc tactatcgcg accgtttaca acccatcgtg gatgccgaaa cgaaaggttt
     1561 gcccattttt gttatccgct cgaactcaat gccccaaatc gagcaattcc tggcggatac
     1621 atttggactt gccacaccac aggaaaaacg tgaaaaagat gacgaagccc tggcccaggt
     1681 tgacatcgcg gttgatttgc ttcaggcggg tgagccttat gttgatttgc agccaactgt
     1741 tgcccacatc cgcaaaagac aacacgaact ggccagagaa tacggcctgg tatcgcagtc
     1801 ttttggaaaa gaccccaacc gctacgtgag aatatatcga gattaatatg tttataacgc
     1861 ttgaaggac
//
LOCUS       DAOSFC010000266         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_63871_length_1849_cov_5.298216, whole genome shotgun sequence.
ACCESSION   DAOSFC010000266 DAOSFC010000000
VERSION     DAOSFC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63871_length_1849_cov_5.298216"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PK791_06325"
     CDS             complement(<1..317)
                     /locus_tag="PK791_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOH92962.1"
                     /translation="MRDIETIHVDDDILVINKPSGMLSIPDGYKPDIPHLRSLLEPKF
                     GRLWMVHRLDKESSGVMVLARNAQAHRLLNQQFKDRLVNKIYHALVSPRPEWQSQNVD
                     FPLA"
     gene            complement(341..1195)
                     /locus_tag="PK791_06330"
     CDS             complement(341..1195)
                     /locus_tag="PK791_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOH92963.1"
                     /translation="MIKIIADTTCGIPVATLKAAGIEVLPQIITFGNDSYRDDTELDT
                     ATFLSKLRKAKELPKTAAPPHSLYTPIYEKILNAGDSALVITPSAEVSGTYRSAMIAA
                     KEFDTDRIQIVDTRTIAGGLGSIVLQAQKWSNEGIGMEELVGKVQSMLKREYAFFIVP
                     TLDYLLKGGRIGGAAKLVGTLLKIVPILTVDDGKVATFEKVRTLRQAKKRFINLIVEA
                     CKNNPDPHLSISHCDNIKGARELKSAVEKQLRIKDIPIYLVPPAIVVHAGPDLLSMSC
                     FLTQEKHL"
     gene            complement(1270..>1849)
                     /locus_tag="PK791_06335"
     CDS             complement(1270..>1849)
                     /locus_tag="PK791_06335"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOH92964.1"
                     /translation="HGDIHPEDFANSLMASFDRGHYQVQKFGNPDHLAVQIATSPGKR
                     AGKTALTANIMRVPDGVSVTISNQEWIGLAASLGATALTALRNPFALLTRLDLIAQDI
                     ESVQLEDKVWETIDTTAKQLGTGFTLSERLNRSVCPYCTTANQVSVSHCIACGAPLGE
                     LVPKTCPNCGFVVNHNEESCPNCQFQFDQQFL"
BASE COUNT          511 a          385 c          431 g          522 t
ORIGIN      
        1 gctaaaggaa agtcaacatt ttgcgattgc cattcaggcc gcgggctgac taaggcgtga
       61 tagattttat tgacgagacg gtctttgaat tgctggttta aaagtcggtg cgcctgagcg
      121 tttctggcga gcaccatcac accactgctt tctttatcca atcgatgcac catccaaaga
      181 cgtccaaact ttggttcaag taaactgcga agatggggta tatctggttt gtaaccatct
      241 gggatagata acatgccact tggcttattg atgaccaaga tatcgtcatc gacatgaatg
      301 gtttcgatat cgcgcatcgt taaatgttac aaataaaatt ctataaatgc ttctcttggg
      361 tgagaaaaca actcatagag agaagatccg gaccagcatg tacgacgatg gcaggtggca
      421 caaggtagat gggaatatct tttatacgaa gttgtttttc aacagcactt tttagttccc
      481 tggcaccttt aatgttgtcg cagtggctga tagaaaggtg gggatcgggg ttgttcttac
      541 aagcttcgac aatcagattt ataaaacgtt ttttagcttg tctgagagta cgcacctttt
      601 caaaagtagc aactttgcca tcatcaacag tcaaaattgg aacaattttt aataatgtac
      661 ccacaagttt tgcggcacca ccaatacggc cacctttaag aaggtagtcg agtgttggca
      721 caataaagaa tgcgtactca cgctttaaca tcgattgaac cttaccgaca agctcttcca
      781 tgcctatgcc ttcattcgac catttttgag cttgtaagac aattgaaccc aatccgccag
      841 ctatggtacg tgtatcgaca atttgaatgc ggtccgtgtc aaattctttc gcggcaatca
      901 tcgcgctacg ataggtgcca ctaacttctg ctgatggtgt gatgactaag gcagaatcgc
      961 cggcattaag gattttttcg tagatagggg tataaaggga atgaggtgga gcggcagttt
     1021 taggaagttc tttggccttg cgtagtttac tgagaaaagt tgctgtgtct agttcggtgt
     1081 catcacgata ggagtcgttt ccaaaggtga tgatttgggg caaaacctca atgcctgcgg
     1141 cttttagcgt ggctaccggg ataccacagg ttgtatcagc gatgattttg atcatattat
     1201 cctcaataag gtcttttccg gcaaagccgg ttgaataata cgagcataaa gaccaatagt
     1261 aatattgtac taaagaaatt gctggtcaaa ctgaaactga cagtttggac agctttcttc
     1321 attatgatta acaacaaaac cgcagtttgg acacgttttt ggaactaatt cacccaaagg
     1381 cgcgccacaa gcaatacaat gactgacact cacttggttg gcggtagtgc aatagggaca
     1441 gacgctccga ttgagacgtt cggaaagcgt aaaacctgtg ccaagctgtt tggccgtggt
     1501 atctatggtt tcccatactt tatcttcaag ctgaacagat tcaatatcct gtgcaataag
     1561 atccaatcgg gtgagaagcg caaaagggtt ccgtaatgca gtcagagctg tggcacctaa
     1621 actggcggca aggccgatcc attcctgatt gcttatagtc acactaacgc catcaggcac
     1681 acgcatgata ttagctgtga gtgcggtttt acctgctcgt ttaccgggac tggttgcaat
     1741 ttggactgcc aaatgatcgg gattaccaaa tttctggact tgatagtgtc ctcgatcaaa
     1801 gcttgccatc aaactgtttg caaaatcttc agggtgaata tctccgtga
//
LOCUS       DAOSFC010000267         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_64040_length_1846_cov_3.750977, whole genome shotgun sequence.
ACCESSION   DAOSFC010000267 DAOSFC010000000
VERSION     DAOSFC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64040_length_1846_cov_3.750977"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /gene="pcp"
                     /locus_tag="PK791_06340"
     CDS             complement(<1..586)
                     /gene="pcp"
                     /locus_tag="PK791_06340"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:NF009676.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HOH92965.1"
                     /translation="MKILVTAFEPFDKEPINPTMLMLERLPADFDGHEIMKQVLPTTF
                     GESAEIALVRITEFEPDAVLCLGQAGGRAAMTLERVAININDASIPDNAGAQPVDEPI
                     VPDGPVAYFSTFPNKAIVSAMREAGIPAAISNSAGTYVCNQILYAVLHFIAENSLPAK
                     AGFMHVPYLPVQVADKPNMPSMSLVDMVRALEIAI"
     gene            complement(700..1719)
                     /locus_tag="PK791_06345"
     CDS             complement(700..1719)
                     /locus_tag="PK791_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOH92966.1"
                     /translation="MKNRKLMPHTQETETRQMVVNLAQDFLKAGLVVRTWGNFSARLD
                     KDTFVITPSGKAYEGMQTSELVTVATADGKPLAKGSGKPSSESPMHARVYQRYPKIKV
                     VAHTHQVYASALTLLGTDIDVPEKWQSILGTTRIPLSAYALPGSRALHNNMADTLARS
                     DVRIVLMGRHGAFILAETATECLRLAEDMERFCTEVYAERLGKAITHRPDIQSDNDRE
                     KSLAQKWQKTTQEGVAVSADSEVLPWLSGTLNPYLDDFAQICGVSVSQRRGSASVVFD
                     PDAQVAICFGKDEADAKNVRAVLEKNARAANIARLSGQKPIPAWESLAMRLVYQLKYS
                     KQANR"
BASE COUNT          444 a          478 c          456 g          468 t
ORIGIN      
        1 gaatcgcaat ctccaaagcg cggaccatgt caaccaggct catggatggc atgttgggtt
       61 tatccgccac ctgaaccggc agatagggca catgcataaa acccgctttt gctggcaaac
      121 tgttttcagc gataaagtgc aaaaccgcgt atagaatctg gttacaaaca taggtgccgg
      181 cagaattcga gattgccgcc gggatacctg cctcacgcat cgcgctaaca atcgccttgt
      241 tggggaacgt gctaaagtat gccacgggtc catcaggcac aataggctca tccacaggtt
      301 gcgcgccggc gttatctgga atgctcgcat cattgatgtt aatcgccacc cgttcaagcg
      361 tcatcgccgc gcgcccaccc gcttgcccca aacacaaaac cgcatctggt tcaaactcgg
      421 taatacgcac caaagctatc tctgctgatt cgccaaaagt tgtaggtaga acttgcttca
      481 taatctcgtg tccatcgaag tcggcaggca aacgctcaag cattaacatc gtggggttaa
      541 tcggttcctt gtcaaaaggt tcaaaggcgg taactaaaat tttcataatg actccgtatt
      601 atgcttgtta taaaatagta tacctgagtg gatgcatata aacatcaaga ccacccaaaa
      661 gtgtggtggt cttgatttct taatttaaaa tgatggcctt taacggtttg cctgtttgga
      721 atatttcaat tgatagacca aacgcatggc aagggattcc caggcaggga tgggtttttg
      781 accgcttaac cgcgcgatgt tggcagctct ggcattcttt tccaaaacag cacggacatt
      841 tttcgcgtcg gcttcatcct tgccaaaaca aattgccacc tgtgcatccg ggtcgaaaac
      901 aacactggca gacccacgcc tctggctaac gctaaccccg caaatttgcg cgaagtcatc
      961 cagatagggg ttcaaagtgc ctgaaagcca gggcaggact tcgctatctg cagatacggc
     1021 aacgccttcc tgcgtcgttt tttgccattt ctgggcgagg cttttttctc tgtcgttgtc
     1081 gctttggata tccggtcggt gtgtgattgc ttttccgaga cgctccgcgt aaacttccgt
     1141 gcaaaaacgc tccatatcct cagcaaggcg gagacattct gttgcggttt cggctaagat
     1201 gaaagcgcca tgcctgccca tcagcacgat tcgcacgtcg ctgcgggcaa gggtatcggc
     1261 catgttgtta tgtaatgctc tactgccggg taaagcataa gcgctcaagg ggatacgcgt
     1321 ggtgcccaaa atgctctgcc atttttcggg aacgtcgata tccgtcccca aaagggtcag
     1381 cgcggaggca tagacctggt gtgtatgcgc cacaactttt attttaggat aacgttgata
     1441 gacccgggcg tgcatcggag actcagaaga aggcttacca ctgccttttg ccaggggttt
     1501 tccatctgcg gtcgcgacgg taacaagttc gctcgtttgc ataccctcgt atgctttgcc
     1561 cgaaggagta atgacgaatg tgtctttatc cagacgggcg ctaaagttgc cccatgtgcg
     1621 tacaaccagt cccgctttga gaaaatcctg cgccagattg acaaccatct ggcgtgtttc
     1681 tgtttcctga gtgtgtggca tgagtttcct atttttcaaa aggaacaacg tgctcaaaaa
     1741 cacggtcgaa agcagccagt gcctgatcaa ttttctcttc gtcgtaggct gcgctggtgt
     1801 agaggcggct accagcaagc gttaccaggc cttccgccat atacgc
//
LOCUS       DAOSFC010000268         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_64349_length_1840_cov_5.872269, whole genome shotgun sequence.
ACCESSION   DAOSFC010000268 DAOSFC010000000
VERSION     DAOSFC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64349_length_1840_cov_5.872269"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PK791_06350"
     CDS             <1..253
                     /locus_tag="PK791_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92967.1"
                     /translation="GDSQAWEPEQKLEIKMTGVKAGDVVKLIGPDFTHEFPPVETKGE
                     FEAFFTPQKPGFVRLELWQSLYGILPPMPILISNPIWMD"
     gene            260..1240
                     /locus_tag="PK791_06355"
     CDS             260..1240
                     /locus_tag="PK791_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH92968.1"
                     /translation="MTKTDSLSPDTDNKSVRRRFPNVIFLFLCAIIWGFGFIAQRLSN
                     QHVGPFTFNGIRFLLGALTVSPLLFIVRRAEKKDLSASEISATEARNKTRAMRRAWII
                     GSLICGLALFAGASFQQIGLINTEAGKAGFITALYIILVPLIGLFLGKRVSWNVWIGL
                     ALAVGGLYLLMVEGDFSVAPSDLIILIGAFLWALHILAVDHHTEKVDGIRLAFGQFLV
                     SGLLSFGVALVRETISWPAIQEALLPLLFSGVVVAGVAFTFQVLGQRGTNPTLAALIM
                     SLEAVFAVIGGILVLNERMSSREWVGSALMLTALIIAQLKFVPHKNKAAD"
     gene            1371..>1840
                     /locus_tag="PK791_06360"
     CDS             1371..>1840
                     /locus_tag="PK791_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOH92969.1"
                     /translation="MSILSNVMGQYNTKLDNTAIEDFPRYDFATRIKPQVRWLTPVTW
                     LLSYPDVWKHKVKIHRDPRLKDIKPPYLLICNHNSFTDFKVMTAAIFPHRANYVVALD
                     GFIGREWLMRKVGCIAKRKFVNDVQIIRQTQHLLSKGQIVTYYPEARYSLIGTPS"
BASE COUNT          455 a          431 c          433 g          521 t
ORIGIN      
        1 aggtgacagc caagcctggg aaccggaaca aaagcttgag attaaaatga cgggagtaaa
       61 agcaggagac gtggttaaac tgattgggcc tgactttaca catgaattcc cgcctgttga
      121 gacaaagggc gagtttgaag cctttttcac gccccagaag cccggttttg tgcgtttgga
      181 gctctggcaa tccttatatg gcatcttacc acccatgccg atactaattt cgaacccgat
      241 ttggatggac tgatatttta tgacgaagac tgattcgctt tcgcctgata cagacaataa
      301 aagcgtcaga aggcgttttc ccaacgtcat cttcctgttc ctgtgcgcga tcatctgggg
      361 ctttggcttt atcgcacaaa gactgagtaa tcaacatgtt ggtcctttta ccttcaatgg
      421 catccgcttt ttgcttggcg cgcttactgt ttcgccactt ttgtttatcg tgcgccgtgc
      481 tgaaaagaaa gatcttagcg catcggaaat cagcgcgacc gaggcacgaa ataaaacacg
      541 tgccatgcgt cgcgcctgga taataggcag tttgatatgc ggactggcac tttttgccgg
      601 agcgagtttt cagcaaatcg gtttaattaa caccgaagcc ggtaaagctg gattcatcac
      661 cgcgttgtat atcatcctgg taccactgat tggtttattt ttgggcaagc gggttagctg
      721 gaacgtgtgg attgggctgg ccctggcagt gggcggtttg tacctgctga tggtcgaagg
      781 tgattttagt gtggcaccaa gcgacttaat cattttaatt ggcgcattct tatgggcact
      841 gcacatcctg gctgttgacc accataccga aaaagttgat ggcatccgtc tggcattcgg
      901 gcaatttttg gtaagcggct tactctcatt tggcgtggca ttggtacgcg aaacgataag
      961 ctggcctgcg attcaagaag cgctgttgcc ccttttgttt tcaggcgtcg tggtcgcggg
     1021 tgtggcattt acttttcagg ttttaggtca acgtggcacc aaccccacgc tcgcagcact
     1081 cattatgagt cttgaagcgg tctttgctgt gattggcggt attttagttc taaacgaacg
     1141 catgagttca cgggaatggg ttggcagtgc tttgatgctt accgccctta tcatcgcaca
     1201 gctgaagttt gtaccccaca aaaataaggc tgctgattga ctaccctata gctaatatca
     1261 aatttattta atactcattt gcaactaatc tgccccattc gggttgatta ttttgcagtc
     1321 tcaagctatt atcaaatctt atttacaatt tcaattagga ggagtgatta atgagcattc
     1381 ttagtaatgt gatgggacaa tacaatacta aactggacaa caccgcaatt gaggattttc
     1441 cgcgttatga cttcgccaca cgcatcaaac cccaggtacg ctggctcaca cccgtaacat
     1501 ggcttctgag ctatccagat gtatggaagc acaaagtaaa aatccatcgc gacccacgcc
     1561 ttaaggacat caaaccaccc tacttgctta tttgcaatca caattccttt actgacttta
     1621 aggtaatgac cgctgccatc ttcccacacc gtgctaatta tgttgttgcg ctggacggtt
     1681 tcattggccg tgaatggctc atgcgcaaag ttggctgcat cgccaagcgt aaattcgtaa
     1741 acgacgtgca aatcattcgc caaacacaac atctgctcag caaagggcag attgtgacct
     1801 attatcctga ggcacgatat tcgcttattg gcacaccttc
//
LOCUS       DAOSFC010000269         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_64501_length_1837_cov_5.030303, whole genome shotgun sequence.
ACCESSION   DAOSFC010000269 DAOSFC010000000
VERSION     DAOSFC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64501_length_1837_cov_5.030303"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="PK791_06365"
     CDS             <1..921
                     /locus_tag="PK791_06365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH92970.1"
                     /translation="ETILPRFDYLLQRDKPAVVKNKRNQAMTQRGLEALAGDACKAII
                     AWSENAAKNQRNLLSEFSSEIAEAILSKMIVLAPPQDALVDEVTVRTYSKENPIRFIL
                     VGAGFFRKGGREILNSFEKLVKEEELPIKLVLVSRLHRDYYAAHETEADIAWAKEKIQ
                     TNSAWIEYYETMPNQQVLELLKTCDVGLLPTYADTYGFSALEAQSAACPVISTDVRAL
                     PEINNTEVGWLINVPKNELGEAYYQTPEDRQVLSERIQSGLEHIIRGIVQEPTTIAKK
                     GQAALEKIKAQHDPVRYGEVLREIYEKAVA"
     gene            1051..>1837
                     /locus_tag="PK791_06370"
     CDS             1051..>1837
                     /locus_tag="PK791_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOH92971.1"
                     /translation="MIVTTKKLFEHAYGKYAIGAYNINNLEQIEGLFRGNLQSQAPFI
                     IQISKGARSYTNKTFLEGLIRAADEIYPEAIFAVHLDHGDEQTCYDCIDSGFYSSVMI
                     DGSSLPYEENVAVTRRVVEAAHAKGIVVEAELGMLGGVEEHVKVDEADAKLTDPDQAK
                     DFVERTGCDSLAVAIGTSHGAFKFTGSQSLHFNVLADIQKNLPGFPLVMHGSSSVPEE
                     EVARINAAGGHIAGAKGVDATQFKAASELGVVKINIDTDGRLVW"
BASE COUNT          511 a          406 c          467 g          453 t
ORIGIN      
        1 gagactatcc ttccccgctt tgattacctt ttgcagcgtg ataagcctgc tgttgtaaag
       61 aacaaacgca accaggctat gacccaacgt ggtttggagg ctctcgcggg tgatgcgtgt
      121 aaggccatca ttgcctggtc agagaatgcg gctaaaaacc agcgcaattt gctttcagaa
      181 ttctcgtcag aaattgcgga agctattcta tcgaaaatga ttgttctggc accaccgcag
      241 gacgcattgg tagatgaagt gaccgtcaga acttatagca aggaaaatcc gatccgcttt
      301 attctggttg gcgcgggttt tttccgcaaa ggcgggcgcg aaattttgaa tagctttgaa
      361 aagcttgtta aggaagaaga acttcctatt aaacttgtgt tggtcagtcg cttgcatcgg
      421 gactattacg ccgcgcacga gacagaggcg gacatagcct gggcaaaaga aaaaatccag
      481 acaaattcag cctggattga gtattatgaa acaatgccta atcaacaagt gctggaactg
      541 ctaaaaacct gtgatgttgg tctgttgccg acctatgctg acacttatgg cttttcagcg
      601 cttgaagcgc agtccgcggc ttgtccggtc atcagtacag acgtgcgcgc tttgccagag
      661 atcaacaata cagaagttgg ctggttgatt aatgtgccga aaaatgaact gggagaagcg
      721 tattatcaaa caccggaaga ccgtcaggtt ctaagcgaac gcattcaatc ggggcttgaa
      781 catatcatta ggggaatcgt ccaggagcca accactatcg ctaaaaaggg gcaagccgcg
      841 cttgaaaaaa tcaaggcgca acatgaccct gttcgctatg gggaagtctt gcgggaaatt
      901 tacgaaaaag ctgtagcgta aggcttatct tcttcagttg cgcgacaagt cgcaaattaa
      961 tgtaatatcg gaaaatgcct gacttaagga tgaaatcagc agctttatta ggtataatca
     1021 aagtatcgaa attgcattca aggagacaaa atgattgtta ccaccaaaaa actattcgaa
     1081 catgcctatg gaaaatatgc cataggtgca tataacatca ataaccttga acaaattgaa
     1141 ggtttgttca ggggtaatct gcaatcgcag gcacctttta tcattcaaat cagcaagggc
     1201 gcgcgctctt acaccaacaa aacattcctg gaaggactca tccgcgcagc tgatgaaatt
     1261 taccctgaag ccatttttgc tgtccatctt gaccatggcg acgagcaaac ttgctatgac
     1321 tgtattgatt cgggctttta cagctcggtg atgattgacg gttcttcctt accctacgaa
     1381 gaaaacgttg ccgtaacccg ccgtgtggtc gaagccgcgc atgccaaagg tattgtggtt
     1441 gaggctgaac tgggtatgtt gggtggcgtt gaggaacatg tcaaagttga tgaagctgac
     1501 gccaaattga cagaccctga ccaagccaag gattttgttg aacgtacagg ctgcgactcg
     1561 ctggctgtcg cgattggcac gagccatggc gcgtttaagt tcactggttc acaaagtttg
     1621 catttcaatg tcttggcgga tatccaaaag aacctgcccg gtttcccgct ggtgatgcac
     1681 ggttcaagct ctgtgcctga agaagaagtg gcgcgcatca atgctgccgg cggtcatatc
     1741 gctggtgcca aaggcgtgga tgccacgcag tttaaagccg catcagaact tggcgtagtg
     1801 aaaatcaaca tcgataccga tggacgcctg gtgtgga
//
LOCUS       DAOSFC010000270         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_65599_length_1817_cov_5.604994, whole genome shotgun sequence.
ACCESSION   DAOSFC010000270 DAOSFC010000000
VERSION     DAOSFC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65599_length_1817_cov_5.604994"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PK791_06375"
     CDS             complement(<1..403)
                     /locus_tag="PK791_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92972.1"
                     /translation="MNVHPLAGKTAPHDFLVNVPELIARYYLDKPEPGVPAQAVSFGT
                     SGHRGTSLNVSFNENHVLAISQAIAQYRQSAGITGPLFMGMDTHALSEPALYSAIEVL
                     AANGVELRLHQGNQYTPTPVISHAILTWNEAH"
     gene            complement(415..1002)
                     /locus_tag="PK791_06380"
     CDS             complement(415..1002)
                     /locus_tag="PK791_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH92973.1"
                     /translation="MTKLNVKDYRNPDYPIEDMILNRWSPRAMSGEPIDAEQLMSLFE
                     AARWAPSALNLQDRFYLYAYRETSDFELFFQLLAKGNQAWCDKAAVLIVAFSDSQLPD
                     GKPNPWHALDAGLAIENLLLQCSAMDLAGHPMTGFDAEKASQDLNVPERFVPQCMIAV
                     GHPGDPSQLSEFNQAREFPNQRKPLSDFIREGKYQ"
     gene            complement(1317..>1817)
                     /locus_tag="PK791_06385"
     CDS             complement(1317..>1817)
                     /locus_tag="PK791_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOH92974.1"
                     /translation="ITDALLKKGIEAKDIQTANFNVYPMNRYDNMGNMTGVEYSVDNT
                     LYVTVRDLKSLSEILDAAIKAGANQINGISFDIEDRTAAQKQARDLALQDAEAKAQEV
                     ADTVGVTLGEIQIINVSNMSYVEPYPMYGMGGGRAMDMVAESSVPVSAGQIVVTYEVS
                     LVYRIK"
BASE COUNT          464 a          432 c          435 g          486 t
ORIGIN      
        1 gatgggcttc attccaggtg agaatggcat gcgaaataac cggcgtgggc gtgtactgat
       61 tgccctgatg caagcgcagt tcaacaccgt tagcagccaa aacttcaatc gccgaataaa
      121 gcgcgggttc ggaaagcgcg tgggtatcca tgcccataaa aagtgggcca gtaataccgg
      181 cactttggcg atattgagcg attgcctggc taattgccaa aacgtgattt tcattaaaac
      241 tgacattaag cgacgtgcca cgatgacctg acgtgccaaa gctgactgcc tgggcaggca
      301 cgcctggctc gggtttgtca agataataac gcgcgatgag ttctggtaca ttcaccaaaa
      361 aatcatgtgg ggcggttttt ccggcaaggg gatgaacgtt catttttgac ctctttattg
      421 atatttacct tcacgaatga agtctgataa tggcttgcgc tgattgggaa actcgcgggc
      481 ctggttgaat tctgaaagct ggctggggtc gccagggtgt ccgacagcga tcatgcactg
      541 tggcacgaaa cgttcaggga cgtttaaatc ctgggatgct ttttcagcgt caaaacccgt
      601 catgggatgc cctgccaaat ccatcgcgct gcattgcaag agcaggtttt caatcgccag
      661 acccgcatcc aaagcatgcc agggatttgg cttgccatcg ggcaattgag aatctgaaaa
      721 cgcgacaatt aataccgcgg ctttatcgca ccaggcttgg tttccttttg ccaaaagctg
      781 gaagaaaagt tcaaagtcac ttgtttcacg ataggcgtac aagtaaaagc ggtcttgaag
      841 attaagagca gatggcgccc aacgcgcggc ttcaaaaaga ctcatcagct gctcagcatc
      901 gataggctcg ccactcatcg cccgcggtga ccagcggttt agaatcatgt cttcaatggg
      961 gtaatctggg tttcggtagt cttttacgtt gagttttgtc atgaaaagct ctccggtttt
     1021 atggttttaa aagcatgtgc ccgattaaaa cgcagacacg ggttgaaaac aaaaagcctg
     1081 cacacaccag acgtggttac aggcttttct gtctttattt ttagcgggaa caaaggggtt
     1141 ttcatgcctt tattctagca taatcgtaaa gctttcccta ctttattaag caattttttg
     1201 ttttttaagg acatggtcag gagaccatgc ccgaacaggt tcacaccaca tgttcaatcg
     1261 tggtctcctg accacgggca acatctttat tacgcgctat ttacaaaaat tcgtccctat
     1321 tttatgcggt acaccaagct gacttcataa gtcacgacaa tttggccagc agatactgga
     1381 acggatgatt cagccaccat atccatagcc ctgccaccgc ccataccata catcgggtag
     1441 ggctcaacgt aggacatatt actcacattg atgatttgaa tctcgcctaa agtcacacca
     1501 actgtatccg caacttcctg cgcttttgct tcggcatcct gcagtgccaa atcgcgggct
     1561 tgcttttgcg cggcagtgcg gtcttcgata tcaaaactga tgccgttaat ttggttcgcg
     1621 ccggctttga tggctgcgtc taaaatttca ctcaaggatt ttaagtcacg cacggtgaca
     1681 taaagcgtgt tatccacaga gtactcaaca ccggtcatat tgcccatatt gtcatatcga
     1741 ttcatcggat agacgttgaa gttagcggtt tgaatgtctt tggcttcaat gcctttctta
     1801 agcaaagcat cggtaat
//
LOCUS       DAOSFC010000271         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_66101_length_1808_cov_2.786081, whole genome shotgun sequence.
ACCESSION   DAOSFC010000271 DAOSFC010000000
VERSION     DAOSFC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66101_length_1808_cov_2.786081"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PK791_06390"
     CDS             <1..339
                     /locus_tag="PK791_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH92975.1"
                     /translation="RPMTMSESYHFLHYFLFDEDQVILPSASLSFGQRSRLMLACLVA
                     EGANVLVLDEPVNYLDIPSREQFEQALQAFTGGVLLVAHDRAFIFRVTEKTWLLENGK
                     IKVGWSEDLV"
     gene            complement(452..1354)
                     /locus_tag="PK791_06395"
     CDS             complement(452..1354)
                     /locus_tag="PK791_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92976.1"
                     /translation="MKWLTLRQNSIAIIIMTVFLLFFGILAINAATSLRDYPADKSLL
                     ATALAQSYPVSIVITLPALMGAFIAAPLISKEIENNTLQLAFTQSITRRKWFWDKFGT
                     LFILIVVVFGLFSLSQHYFYLNAVKLTNSWDWFDIQIVVYMAYVVLAFSLGTLTGLLI
                     GKQVPSMAIFLVLFFLIRFALFKMRAYWFKPLVATWNFIGENPEYQKGIWVISSRLVD
                     KLGNPVTVGLKQLGCDVLQFQVGDVSSTVEYDCYQRAGLLMEYTYQPIERFLHIQVIE
                     VTITLLISFLAIALMLWILEKRKR"
     gene            complement(1351..>1808)
                     /locus_tag="PK791_06400"
     CDS             complement(1351..>1808)
                     /locus_tag="PK791_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92977.1"
                     /translation="MDEPFANLDPLARHQLLQLISETTIEEGHSVLISTHDITELEKV
                     CDTLVLINRSRVLLCGDIETLVATHRWITLTPEEAEKEVNTLHVLSIEKAQRAHRLLV
                     RINGITEFFRMPEPVENVNLEELILAYLALNHQEVESLNSKLTHQELLL"
BASE COUNT          538 a          365 c          409 g          496 t
ORIGIN      
        1 cgaccaatga cgatgtcgga aagctatcac ttcctgcatt actttttgtt tgatgaagac
       61 caggtgattt tgcctagtgc cagtttgagc tttggacaac gctcgcgctt aatgctggcg
      121 tgtttggtag cggaaggcgc gaacgttttg gtgctggatg agccggtgaa ttatttggat
      181 atcccttcgc gcgaacagtt cgagcaagcc ctgcaggctt tcacgggcgg ggtgcttttg
      241 gtagcccacg accgcgcttt catcttccgc gtgacagaaa aaacctggct cttggaaaat
      301 ggcaagatca aggtcggctg gtcggaggat ttggtctagg ctgatatgag ctacacctta
      361 ctagtgcgtg gcgttccgat atgcaacttt tcagcctatg acgagacaac gtcacatggt
      421 aatagtactg aattttactg tgctcttcac attatcgctt ccttttttca agaatccaca
      481 acatcaaagc tattgcgaga aatgaaatga gaagggtaat tgtgacttca atgacttgaa
      541 tatgcaaaaa acgttcaatg ggttggtaag tatattccat caacaagcca gcgcgttgat
      601 agcagtcgta ttcgactgtg cttgatacat cacctacctg aaattgtaga acatcacaac
      661 caagttgttt caatccaacg gtgacaggat ttcctaactt atctaccaga cgggatgaaa
      721 taacccagat gcccttctga tattcgggat tctctccaat gaagttccag gtggcaacca
      781 atggcttgaa ccagtacgct cgcattttaa acaaagcaaa acggataagg aaaaacagta
      841 ccagaaaaat cgccatggat ggaacttgct ttcctattaa caaaccggtt aaggtgccta
      901 gtgaaaacgc caaaacgaca taagccatat aaaccacaat ttgaatgtca aaccagtccc
      961 atgagttggt tagtttgaca gcattcaaat aaaagtaatg ttggcttaag gaaaacaaac
     1021 caaacacgac cacaataagg atgaataaag tgccaaactt gtcccaaaac catttcctgc
     1081 gggtaatgct ttgagtaaaa gcaagttgga gcgtgttgtt ttcaatttct ttggaaatca
     1141 atggtgccgc gatgaacgcg cccatcaagg ctggtaaagt aatgacaatg gataccggat
     1201 aagactgggc gagcgctgtt gctaaaagtg atttgtcggc aggataatcc cttaaagatg
     1261 tcgcggcatt aattgccaga ataccaaaaa acaataagaa aacagtcatg atgatgattg
     1321 cgatactatt ttgccgtaag gtaagccatt tcataagagg agctcctgat gagtaagttt
     1381 agaattgaga gattccactt cctggtggtt aagtgctaaa taggcgagga ttaattcctc
     1441 caaattgaca ttttcaacag gttcaggcat acgaaaaaat tctgtaatac cattgattct
     1501 gaccaataag cgatgggcgc gttgggcttt ttcaatagat aatacatgta aagtatttac
     1561 ttctttttct gcctcttcag gtgtgagagt tatccatctg tgcgtggcca ctaatgtttc
     1621 aatatcgcca cacagtaaaa ctctggatct gttgattaag acgagtgtgt cacaaacctt
     1681 ttcaagttcg gtgatgtcat gtgtggagat aagcactgaa tgaccttcct caattgtggt
     1741 ttcagatatt agttgcaaaa gttgatgacg tgcaagtgga tccaaattcg cgaaaggttc
     1801 atccatca
//
LOCUS       DAOSFC010000272         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_66248_length_1805_cov_4.838857, whole genome shotgun sequence.
ACCESSION   DAOSFC010000272 DAOSFC010000000
VERSION     DAOSFC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66248_length_1805_cov_4.838857"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PK791_06405"
     CDS             <1..954
                     /locus_tag="PK791_06405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOH92978.1"
                     /translation="FSIAILPLLIAGGYFVINAKRIPAFLLPLITVLALSVAMLWILK
                     LGLGDSWGANVKRDSGLFSFSMSFSTFYILLLQIFIFAIVFAIAMAFFFKNVDFGAIF
                     ENLIINPEMQIEDFAIDSDLVLLVLFMVAFSVPLIEELFKTIGVWFLKPRKISPREGW
                     IAGLMSGAGFGLIEAYLYSMQGVMMTTFPDWLYFILGRTGGLLLHTFAGGIVGWGLAK
                     SWREKRPTYAVGAYIVALLTHSLWNSIAVGQAVLLNQFDIEVPDVAVYISLSVLFVAM
                     LVAYLMMSQNIAREPRESITKDNQARPYLPQDPAGTSNWSV"
     gene            959..1657
                     /locus_tag="PK791_06410"
     CDS             959..1657
                     /locus_tag="PK791_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH92979.1"
                     /translation="MTISRDDLLNIIQKLIDKFCNQKIYNAEIPESLNGALLVTNHIS
                     RLDVVFLMLSTKRKDVIALTASNYRRALFFGTILKKLGVIWLDRGESDFKAMRETADY
                     LKKGWIVGLAPEGRRSRSGKLIKAKPGAVLIAMKTGVPIIPVGLVGTADMGKAIRKWR
                     KMDVTIKFGEAFYLPEREENEHNRDYVERALDEIMCRIAVLIPEEQRGFYADHPRLKS
                     LLAEIEEKKNATES"
BASE COUNT          469 a          390 c          418 g          528 t
ORIGIN      
        1 tttagcatcg caattttgcc tttgctcatc gcgggcggat attttgtaat aaacgctaag
       61 cgtattcccg cgtttttgct ccccctgatt accgtcctgg cattatccgt tgccatgctt
      121 tggatcctaa aactcggatt aggcgattcc tggggcgcga acgttaagcg cgatagcggg
      181 ctcttcagct ttagcatgag ctttagcact ttctatattt tgctgttgca aatcttcatt
      241 ttcgccattg tttttgccat agcgatggca tttttcttta aaaatgttga ctttggcgca
      301 atctttgaaa atttaatcat aaatcctgaa atgcagattg aagactttgc aatagattct
      361 gatttagttc ttcttgttct ttttatggtc gcgttttctg ttcccctcat tgaagaactc
      421 ttcaaaacaa tcggggtttg gttcctgaaa ccccgcaaga tcagcccacg cgaaggttgg
      481 attgctggct taatgagtgg cgcgggattt gggctcattg aagcctatct gtatagcatg
      541 cagggtgtga tgatgaccac ttttcctgac tggctttact tcatccttgg cagaaccggt
      601 ggtttgctct tgcatacctt tgcaggcggg atcgtgggat ggggactggc aaaaagctgg
      661 cgcgaaaaac gcccaacata cgccgttggg gcatatattg tcgctttact tacgcatagc
      721 ttatggaatt ccatcgctgt tggacaagcg gtgctattaa atcagtttga cattgaagtg
      781 cccgatgtgg ctgtttacat cagcctgagc gttctctttg ttgccatgtt ggtggcgtat
      841 ttgatgatga gtcaaaatat tgcgcgtgaa ccgcgtgaat caatcaccaa agataatcag
      901 gcaagacctt acttgccaca agaccctgcg gggacatcca actggagtgt ataagctaat
      961 gactatcagt cgtgatgact tgctaaatat aatccaaaaa ctgattgaca aattttgcaa
     1021 tcagaaaatt tataatgctg aaattcccga atcgctcaat ggtgccttgc tggttaccaa
     1081 ccacatcagc cgtcttgatg ttgtgttttt gatgctttcc acaaaacgca aagatgtgat
     1141 tgccttgacc gcaagtaact atcgcagggc gctctttttc ggtacgatac tcaaaaaatt
     1201 aggggttata tggcttgacc gtggcgaaag cgattttaaa gccatgcgcg aaacagctga
     1261 ctatcttaaa aaaggctgga ttgttggtct tgctcctgaa ggacgacgct cgcgttctgg
     1321 taaattaatc aaagctaaac caggcgcggt gctgattgcc atgaaaaccg gtgtaccgat
     1381 tattccggta gggcttgttg gtacagcgga tatgggaaaa gccatcagaa aatggcgcaa
     1441 aatggatgtc accattaaat ttggtgaagc cttttatttg ccagaacgcg aagaaaacga
     1501 gcacaaccgt gactacgtgg aacgcgcgct ggatgaaatc atgtgtcgca tcgctgttct
     1561 tattcccgaa gagcaacgtg gcttttatgc cgatcatccg cgcctaaaaa gcctcttggc
     1621 tgaaattgaa gaaaagaaaa acgctaccga atcttaggag gcacgatgat tcctattcgt
     1681 gatgaaatta gaaccaaacg tattccttac gtcaattacg cattaatcgc tattaacatc
     1741 attgtcttta tgacgatgtt tgtcaatccg caaaaagcgg aagccatcgt catggaacac
     1801 gcact
//
LOCUS       DAOSFC010000273         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_66343_length_1803_cov_5.883295, whole genome shotgun sequence.
ACCESSION   DAOSFC010000273 DAOSFC010000000
VERSION     DAOSFC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66343_length_1803_cov_5.883295"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PK791_06415"
     CDS             <1..371
                     /locus_tag="PK791_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92980.1"
                     /translation="TDFLTGSDLEASIGLRLPVDKSLNTTLLQALRNLLSLDTQTGDR
                     LLMEISPDRFLRICAAPEPSVYSLSMDDTGELFDRMGRALAVPSDAVGKYVGVRHGKG
                     FLVREMRLDLESGRYQLKTF"
     gene            486..>1803
                     /locus_tag="PK791_06420"
     CDS             486..>1803
                     /locus_tag="PK791_06420"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HOH92981.1"
                     /translation="MIRATFTFPQGFLWGCATSSHQVEGNNINNDWWAWEQEQGHILN
                     GDRSGKACDWWAGRWREDFDRAKETWQNAQRISIEWSRVQPEPDRWDEGALDRYRQML
                     MGLKERGMVPMVTLHHFTNPLWLTEMGGWENDAVVELFGEYTRKTVEALKAHCDFWVT
                     INEPNVYMAGGYLGGGFAPGKNDVKMALQVLINMVKAHAKAYEIIHELQSEAHVGIAH
                     HWRGFMPANKGLLTSRLADLYNTAFDNAFAETLRSGKFDAKIHKEDIPQAKDTQDFFG
                     INYYGRQEVKFDLFKPSSMYSEYGYPKGFELSDSGFVANDPEGFRACIRWAHSFGLPI
                     YITENGTEDSKDAFRRKFLVDHILAMWHEISHNVPVKGYFHWSLVDNFEWNLGWERRF
                     GLWQMDEKTQQRTQRDSGKLYAEICRTNSLSWDAIEKFAPELYEYVF"
BASE COUNT          460 a          392 c          507 g          444 t
ORIGIN      
        1 cgactgattt tctgacgggt tcggatttgg aagcgtccat cggcttgcgc ttgccagtgg
       61 ataaatcgct gaacacaacg cttttgcagg ctttgcgcaa tctattgtct ttagacacgc
      121 aaaccggcga cagactgctg atggagatta gccctgaccg cttcttgcgc atctgtgccg
      181 cgcccgagcc atcggtatat tcgctaagca tggatgacac tggcgagttg tttgaccgca
      241 tgggcagagc cctggcggta cccagcgatg ctgttggcaa atacgttggc gttaggcatg
      301 gcaagggctt tttggtgcgt gagatgcgcc tggatcttga aagtggtcgt tatcagctaa
      361 aaactttcta aacagtagcg aaaacgcaaa gcaacacgcc tggttaaaaa acctgggcat
      421 aaaaacaaaa ggaaagattg tggctttgat ataataaagc catcatcaac catttggagg
      481 tcatcatgat tcgagcaact tttacgtttc cacaaggttt cctttggggc tgtgccacgt
      541 cgtcgcatca ggttgagggc aataacatca acaacgactg gtgggcctgg gaacaggaac
      601 agggccacat tttgaacggc gaccgcagtg gcaaggcttg cgactggtgg gcgggtcgct
      661 ggcgcgaaga ttttgaccgg gccaaagaga cctggcaaaa cgcgcaaaga atctcaatcg
      721 aatggtcgcg cgtgcaaccg gaacctgacc gctgggacga gggcgcgctt gaccgctatc
      781 gccaaatgct gatgggctta aaagagcgtg gcatggtgcc gatggtgacc ttgcatcatt
      841 tcaccaaccc cttgtggctg actgagatgg gtggctggga aaatgacgcg gtggttgagc
      901 ttttcggtga atacacccgc aagacggttg aagcactgaa agcgcattgt gatttttggg
      961 tgacgattaa tgagccaaat gtatatatgg ctgggggcta tctggggggt ggttttgcgc
     1021 ctggcaagaa tgatgtaaaa atggcattac aggtcttgat taatatggtt aaagcgcatg
     1081 ccaaagctta tgaaatcatt catgaactgc aatctgaagc acatgtgggc atcgcgcacc
     1141 attggcgtgg ctttatgccg gctaacaagg gattattgac cagtcggctg gccgatttgt
     1201 ataacacagc ttttgataat gcttttgccg aaaccttgcg ttctggtaaa ttcgacgcga
     1261 aaatccacaa ggaagacatc cctcaggcta aagacacgca ggactttttt ggcatcaact
     1321 actatgggcg ccaggaagtt aaatttgatt tattcaaacc atccagtatg tacagcgaat
     1381 acggatatcc caagggcttt gagctttctg attcaggctt tgtcgcgaat gaccctgaag
     1441 gtttccgggc gtgcatccgc tgggcacata gctttggttt gccgatttac atcacggaaa
     1501 atggcaccga agacagcaaa gacgcgttta gaaggaagtt tttggttgac catatattgg
     1561 cgatgtggca tgagatcagt cacaatgtgc ctgtcaaagg ttattttcac tggagtttgg
     1621 ttgacaattt cgagtggaat ttgggttggg agcgacgctt tgggctttgg cagatggacg
     1681 aaaagaccca gcaacgcacg caaagggaca gtggaaagct ttacgcggaa atttgccgca
     1741 ccaacagttt gagctgggac gccatcgaaa aattcgcgcc ggaactctat gagtatgtct
     1801 ttg
//
LOCUS       DAOSFC010000274         1799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_66551_length_1799_cov_3.976491, whole genome shotgun sequence.
ACCESSION   DAOSFC010000274 DAOSFC010000000
VERSION     DAOSFC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66551_length_1799_cov_3.976491"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /gene="thrS"
                     /locus_tag="PK791_06425"
     CDS             <1..1161
                     /gene="thrS"
                     /locus_tag="PK791_06425"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOH92982.1"
                     /translation="LFSTNDEVGQGLILWHPNGGMVRYQIEKFWDEQHVRNGYDLVYT
                     PHMGKASLWETSGHLGFYKDDMYSPIDVEGQEYYLKPMNCPFHLSIYKSQLHSYRDLP
                     LRYAEKGTVYRYERSGVLHGLLRVRGFTQDDAHHFCRPDQMPDEIDFTLDFSLKMLRA
                     FGFTNFQAYLSTMPEKCVGEPEQWQAAEAALEDSLKRLEVPYEVDAGGGAFYGPKIDI
                     KVKDALGREWQLSTIQFDFNEPERFDISYIGEDGQQHRPYMVHRALLGSMERFFGVLI
                     EHYAGAFPAWLSPMQVAIIPIADRHVEFADEVAKQYKEAGLRVKVDARSDRMNAKIRD
                     AQKEKIPYMLVIGDREMEDRQVALRRRSGENLGAMDVDAFLALALQEIADKI"
     gene            1325..>1799
                     /locus_tag="PK791_06430"
     CDS             1325..>1799
                     /locus_tag="PK791_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HOH92983.1"
                     /translation="MTKYTIYQVDAFTLKPFKGNPAAVCILPVEKDSEWMQAVAQEMN
                     LSETAFLLPKGNDYLLRWFTPTTEVELCGHATIASAHILFENGFYDFDETINFHTASG
                     VIAATFDNGTIELNMPLIKSQDVEIPADVIEVTGLNPVTAALSETNQLILMLGSED"
BASE COUNT          459 a          501 c          434 g          405 t
ORIGIN      
        1 ctcttctcca ccaacgacga agttggccag ggtcttattt tgtggcaccc caacggtggc
       61 atggtgcgct accaaatcga aaaattctgg gacgaacaac acgtgcgcaa tggctacgac
      121 cttgtttaca cgccccacat gggcaaagcc tccctttggg agaccagcgg tcatctcggc
      181 ttttacaagg atgacatgta ctcgccgatt gacgttgaag gtcaggaata ctatctaaaa
      241 cccatgaact gccccttcca cctgagcatc tacaaaagcc agttgcacag ctaccgcgac
      301 ctgcccctgc gctatgccga aaaaggcacg gtctaccgct acgaacgcag tggcgtattg
      361 catggcctgt tgcgcgtgcg cggttttacc caggacgacg cgcatcactt ctgtcgccct
      421 gatcaaatgc ccgacgaaat cgactttacg ctcgacttca gcctcaagat gctgcgcgct
      481 tttggcttca ccaacttcca ggcctatctc agcaccatgc ccgaaaaatg cgttggcgag
      541 cccgaacaat ggcaagctgc cgaagccgcg ctggaagata gcctgaagcg tcttgaggtg
      601 ccttacgaag ttgatgctgg cggtggcgct ttttacggcc cgaagattga catcaaggtc
      661 aaagacgctc tcggacgcga atggcagctt tcgaccattc aattcgactt caacgagccc
      721 gagcgtttcg acatcagcta catcggcgaa gacggtcagc aacaccgccc ttatatggta
      781 caccgcgcgt tgttgggttc catggaacgc ttctttggcg tgctcatcga acattacgcc
      841 ggtgctttcc ccgcctggct ttcccccatg caggttgcca ttatccccat cgccgaccgc
      901 catgtggaat ttgctgacga agtcgccaag caatataaag aagctggctt gcgcgtaaaa
      961 gttgacgcac gctccgaccg catgaacgcc aaaatccgcg acgcgcaaaa ggagaaaatc
     1021 ccctacatgc tcgtcatcgg cgaccgtgag atggaagaca gacaagttgc ccttcgccga
     1081 cgcagtggcg agaacctggg cgccatggat gtggatgcct tcttggccct ggccctgcaa
     1141 gaaattgcgg ataagattta aacgctaaca gcgaatggag cgattagttt ttgaaagact
     1201 aaaccagcga ttctcagctt gagcacttgt aaaaaaaatt aagctgggaa tcgccaggca
     1261 aagtcttttg aaaactgcct tagtgaagta aaatggtaaa aaggaaagtt tcggaggtgc
     1321 cattatgaca aagtacacga tttatcaagt cgacgcgttc accctaaagc cttttaaggg
     1381 caatcccgcc gccgtttgta ttctccctgt tgaaaaagac agcgaatgga tgcaagccgt
     1441 agcccaggaa atgaaccttt cagaaaccgc cttcctttta cccaaaggca acgattattt
     1501 gctgcgctgg ttcacgccta ctacagaagt cgaactttgc ggtcacgcca ccatagccag
     1561 cgcgcatatt ctctttgaaa atggctttta cgattttgat gaaaccatca atttccatac
     1621 tgccagtggc gtaatcgcgg caactttcga caacggcacc atcgaactca atatgcccct
     1681 gatcaaaagc caggatgtag aaatacccgc ggatgtcatt gaagtcaccg gtctgaaccc
     1741 agttaccgct gccctttctg agaccaatca gcttattttg atgctgggct ccgaagatg
//
LOCUS       DAOSFC010000275         1789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_67140_length_1789_cov_3.380046, whole genome shotgun sequence.
ACCESSION   DAOSFC010000275 DAOSFC010000000
VERSION     DAOSFC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67140_length_1789_cov_3.380046"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1262
                     /locus_tag="PK791_06435"
     CDS             195..1262
                     /locus_tag="PK791_06435"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOH92984.1"
                     /translation="MQELIVGIHAHTTFSDGAGTHAELAHAAMKAGLDVLITTDHNIL
                     VQGMDGYLQKGRRKMLLLAAEEIYDPTRQPGGNHMLVLGANRELAKFASDPQELIKQT
                     KSANGLSFIAHPNDIALPHANEKAFPWTDWQISGFTGIELWNQLSEFKDRSPSRLKAL
                     FHVLFPNFYTLGPTPATLALWDQLLLSKPHPVVAIAGVDAHATKHHLGPFSRKVFPYE
                     WHFKSLRTHLMTPKKLTGELNADREMIWDALRKGHAFCAYDLPHPSNGFRFTCNTEDG
                     LFWMGDTVSARSGLTFQARLPIRTHLRLIKDGKVIKEHHDREVLTHISKEPGIYRVEA
                     YIDYHNRHRGWIFSNPIYATP"
BASE COUNT          458 a          512 c          404 g          415 t
ORIGIN      
        1 acaatgatcc aaataaccat gaattgcata tttggaactc aaaaacagga gcaccattag
       61 gcagttttaa tgtccccata agtgccagag agctttggtt cacacccgat ggcagcaaaa
      121 tcgttgtttt ggcttctggt gttctataca tcattggcat ccccgaatga aattctgcgg
      181 ggtataattc ctttatgcaa gaactcattg ttggcattca cgcccatacg accttttcag
      241 atggcgcggg gacacacgcc gaattagccc atgcagctat gaaagccggc ctggatgtgc
      301 tcatcaccac tgaccacaac atactggtgc aaggcatgga tggttacctg caaaaaggac
      361 ggcgtaaaat gctcctgctt gccgcggaag aaatctacga ccccacccgc caacctggcg
      421 gtaaccacat gctcgttttg ggtgccaacc gtgaactcgc caaattcgcc agcgaccctc
      481 aggaactcat caaacagacc aaaagcgcca acgggctcag ttttatcgcc caccccaacg
      541 atatcgcact accccatgcc aatgaaaaag cctttccctg gactgactgg caaatcagtg
      601 gttttaccgg cattgaatta tggaaccaac tcagcgagtt caaagaccgc tcgcccagtc
      661 gcctgaaagc gctctttcac gttctttttc ctaactttta tacgctaggg cccacccccg
      721 ccacgctcgc gctttgggac cagcttttgc tcagcaaacc ccacccggtt gtcgctatcg
      781 ccggagttga cgcgcatgcc acaaaacacc acctggggcc tttttcgcgt aaggttttcc
      841 cctacgagtg gcactttaag agtctgcgca cccaccttat gacacccaaa aaactcaccg
      901 gcgaacttaa cgctgaccgg gaaatgattt gggatgctct gcgaaaaggg catgcttttt
      961 gcgcctacga cctgccccac ccgagcaatg gtttccgctt tacctgtaac acggaagatg
     1021 gcttgttttg gatgggcgac accgtttctg cgcgctcggg tctaaccttc caggctcgtt
     1081 tgcctatacg cacccatctg cgtcttatca aggatggcaa agtcatcaag gaacatcacg
     1141 accgcgaagt gctcacccat atcagcaaag aaccgggcat ctaccgcgtt gaagcctaca
     1201 tcgactacca taaccgccat cgtggctgga tttttagcaa tcccatttac gccacgcctt
     1261 agtttgtaat caaaattcag aatatgacat ttaacatggt cagaagaccg cgtgttagtc
     1321 atgggcggga gaccatgaca gaacaagaag attctgcacg gcgagacgcc ggcaggagat
     1381 aatgcccgca ccagcgtttc ttttttcgct cgccccggcg tcccgccgcg ggctaaagtg
     1441 catggcacgg atgccatgca ccagctggac atgctcgtaa gaatcaagac gactggcaca
     1501 tagtgttcgt ttttatccga ttgtgtactc ttttcgcgtt cgcagaggcg tcctgccaac
     1561 gctataaacc caactggtgc gtggcgtccc gccgaagcaa taagaacaaa cgaaaacata
     1621 ttcagcccca cacaaaaaca aagcattcaa tatcactttc gttggtataa ttgaaacgga
     1681 gttctgatga tcaacttttt agccaagatt gaagaaaaaa tccaggcctt gcttgaagac
     1741 gggcttgacc gtcttttgca tcccggcgcg tcttactccc ttagcgcgg
//
LOCUS       DAOSFC010000276         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_68464_length_1766_cov_5.292811, whole genome shotgun sequence.
ACCESSION   DAOSFC010000276 DAOSFC010000000
VERSION     DAOSFC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68464_length_1766_cov_5.292811"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..>1766)
                     /gene="polA"
                     /locus_tag="PK791_06440"
     CDS             complement(73..>1766)
                     /gene="polA"
                     /locus_tag="PK791_06440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOH92985.1"
                     /translation="LVGFSLAASPEKGYYFPVGHRAGKQLSLDELKNAVQPLLSDPKI
                     AKVAHHAKFDLITLRQAGFEINPIRFDTMIAEWLINPDSRNLGLKTLAWVRLGVEMTP
                     IEDLIGKGKNQLSMADLPIARVAPYAAADAVMTLRLMPILKADLKERNAEKLMDDLEI
                     KLVPVLAQMEEYGVLLDAPFFEDFNKELTEMIFALEEDIYRLAGTRFNINSTQKLSDV
                     LFKKLALDPPDPSRKTKSGHYSTSAEILELLRDKHEIVAKILDYREYAKLKSTYVEAL
                     PQQINPKTGRVHSSFNQVGTVTGRIASQNPNLQNIPTRTDLGQRVRQGFIAAPGWKLL
                     SVDYSQVELRIVAHIAQDQAMIEAFRRGQDIHATTAAAITGTPLAEVTPKQRRNAKAV
                     NFGLIYGMSAFGLTRGTDLTLAEAENFIKAYFREFPGVKAWLDMTRVNAAKNGYVETL
                     LGRRRYFPNLAKGSNPQMRQREEREAINAPIQGTAADIVKLAMIALPEALRKAGLNAR
                     MLLQVHDELIFEVPEDELERTVPVVKSLMESAMTLDVPLVCDARAGDNWRELKAV"
BASE COUNT          384 a          428 c          501 g          453 t
ORIGIN      
        1 tattcacggt gtggttctga gtaacgacca caccgtgaat gctttaattc aagggctaca
       61 aacgtaaacg cgctaaacgg ctttcagctc acgccagtta tcgccggctc gggcgtcgca
      121 gacgaggggc acatccagcg tcatggctga ttccataagc gatttaacga ccggtaccgt
      181 tcgttccagt tcgtcttcgg gtacttcaaa tatcagttca tcgtgcactt gcagaagcat
      241 acgcgcgttc aaaccagcct tgcgcaaagc ttcgggcagg gcaatcatgg ccagttttac
      301 aatatcagcc gcggtgcctt gaatgggcgc gttgatagct tcgcgttctt cgcgctggcg
      361 catttgtggg ttggaccctt tggctaaatt agggaagtaa cgccgccggc caagcagggt
      421 ttccacatag ccgtttttgg cagcgttcac gcgggtcata tccagccagg cttttacacc
      481 agggaattcc ctgaaatacg ctttgatgaa attttcggct tctgccaggg ttaaatcggt
      541 gccacgggtc agaccgaaag cgctcatgcc gtaaatcagt ccaaagttca ctgctttggc
      601 gttgcgtcgc tgcttgggtg taacctcagc cagcggggtg ccggttatgg ctgcggcggt
      661 tgtggcgtga atgtcctgac ctcgcctgaa ggcttcaatc atagcctggt cctgagcaat
      721 atgcgccaca atgcgcaact ccacttgcga gtaatcaact gacaggagtt tccagcccgg
      781 cgcggcgatg aaaccctggc gcacgcgctg gcccaagtca gtgcgcgtgg ggatgttctg
      841 cagattgggg ttttgactgg caatgcgccc ggtgaccgtg ccaacctggt tgaaagaact
      901 gtgtactctg cccgttttag ggttgatttg ttgcggtaag gcttcaacgt aagttgattt
      961 cagtttggca tattcacggt aatctaaaat cttggccacg atttcgtgtt tatcgcgcaa
     1021 gagttccaga atctcggcgc tggtggaata atgaccgctt ttggtcttgc gactcgggtc
     1081 gggcgggtct agggcgagtt tcttgaaaag gacatccgaa agcttttgcg tggaattaat
     1141 gttaaagcgg gtgccagcca ggcggtagat atcttcttca agcgcgaaaa tcatttcagt
     1201 gagttctttg ttgaaatctt caaagaaagg cgcgtcaagc aaaacgccat attcttccat
     1261 ttgtgccaga acggggacga gtttgatttc caaatcgtcc atcagctttt cagcgttgcg
     1321 ttctttgagg tcggctttga gaatgggcat caggcgcagg gtcatcaccg cgtccgcggc
     1381 ggcatagggc gcgacacgcg cgatgggtaa gtctgccatg ctgagttggt ttttgccctt
     1441 gccgatgaga tcttcgatgg gggtcatctc aacgcccagg cgcacccagg ccagtgtttt
     1501 gagacccaga ttgcgcgaat caggattgat aagccattcc gcgatcatgg tgtcaaaacg
     1561 aattggattt atttcaaagc cagcctgacg aagtgtgatt agatcaaatt tggcgtggtg
     1621 ggcaactttg gcgattttgg ggtcgctaag taaaggttga acagcatttt taagttcatc
     1681 aaggctgagt tgtttgccgg cacggtgccc gaccgggaag taatagcctt tttccgggct
     1741 ggcagccagg gaaaagccca ccaaat
//
LOCUS       DAOSFC010000277         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_69013_length_1757_cov_3.402468, whole genome shotgun sequence.
ACCESSION   DAOSFC010000277 DAOSFC010000000
VERSION     DAOSFC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69013_length_1757_cov_3.402468"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..1173)
                     /locus_tag="PK791_06445"
     CDS             complement(298..1173)
                     /locus_tag="PK791_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOH92986.1"
                     /translation="MLVSWNYRFRNSPMDHIDPRARFIFSFALLFAATATWNPIILFV
                     LFLIAITWYLMARLTWKDTKVGWFMISIMLFTMIVVNTIITGGGAGGVVPPGGQLVWP
                     GGFDLFGKHFTFGLTVERVWFAISQLMRILSISLAFIIIPYTMDSRAYGVTFSKLGMP
                     DRLAYTMELSFRFIPTLARNFQVTLDAQRARGFELDGSKGNFIKKIRRVAPLLIPVTM
                     NAIVSSEDIANAMDLRGFGEQKRTWLYELKFNWWDWVLIVFSVLMLATVLVLAFRFNM
                     RLFSVPAWFYGLFNK"
     gene            complement(1229..>1757)
                     /locus_tag="PK791_06450"
     CDS             complement(1229..>1757)
                     /locus_tag="PK791_06450"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOH92987.1"
                     /translation="AFGTKQVVYAAIGAALYAVLTWAFNVWQIPGAGNVGIRPAIVIP
                     MFFGIAFGPIVGFITGFVGNILADMLSGYGFWIWWDLGNGIIGLIPGLFSALIVKYDE
                     IKSILWGELSVIVGSAAGMGLASASEFWVSGADFNTVLLANFVPSFVTNIIWGLVLLP
                     LLMIAYAAILKRSGR"
BASE COUNT          538 a          399 c          455 g          365 t
ORIGIN      
        1 catgaacagg gcgacaagca gccaaggaaa gatttttttg aactggtctg attgagtgag
       61 aggagtgtga ttttgattaa tcatagtctt cgccataaaa gtaaattaag aatatcacgt
      121 cactcacaga ccggcaagca atgtcacccc aaaaaaagta aaaaaacttt aatgtcttgt
      181 gttgtatgat gttgtcaaaa aaaaggattt tattgggtgc tctaataaac ttggagcaag
      241 cgaaaactac agcagagatt gtctcacgca aaattgagtg aatttaaatt gaatagttta
      301 tttgttaaac aagccgtaaa accatgccgg cacgctgaac agtcgcatgt tgaaacggaa
      361 tgcgagcacg aggactgtgg caagcatgag gactgagaag acgatgagca cccaatccca
      421 ccagttgaat ttgagctcat aaagccaggt gcgtttttgt tcaccgaatc cacgcaggtc
      481 catggcgttg gcgatgtctt cgctggagac aatcgcgttc atggttaccg ggatgagcaa
      541 gggcgcgaca cggcgaatct tcttgatgaa attccctttt gagccatcaa gttcaaaccc
      601 acgcgcgcgt tgggcgtcta aagtcacctg gaagtttctg gccagggtcg gaataaagcg
      661 gaaagaaagt tccatggtgt aggcgagacg gtcgggcatg cctaatttac tgaaagtaac
      721 accataagcg cgggagtcca tggtgtaggg aatgatgata aaagcgagtg aaatggaaag
      781 gatgcgcatc agttgcgaga ttgcaaacca tacgcgttca acggtcaagc caaaggtgaa
      841 gtgtttgcca aagagatcaa atccacccgg ccaaaccagc tgaccgcccg gaggcaccac
      901 gccaccagcc ccaccgccgg tgatgatggt gttgacgaca atcattgtga aaagcatgat
      961 agaaatcatg aaccagccca ctttggtgtc tttccaggtg agacgggcca tcagatacca
     1021 ggtgatggcg atgagaaaga ggacgaacag gatgatggga ttccaggttg cggtagctgc
     1081 aaacagcaag gcgaacgaga atatgaaacg tgcccgcgga tcaatgtgat ccatgggact
     1141 gttgcgaaaa cgataattcc agcttaccag catttgatac gtccttgagg gcgattctta
     1201 aaaaatcgcc ctcactaaaa acttaaaatt accgaccgct gcgctttagg atggccgcgt
     1261 atgcgatcat cagcaagggc agcaaaacga gaccccaaat gatgttggtc acgaatgagg
     1321 gtacaaagtt ggctaacagg acggtattaa aatctgcgcc ggatacccag aattctgacg
     1381 cactggccag gcccatgcct gccgcgctac ccacgatgac tgaaagctca ccccaaagga
     1441 tggatttaat ttcatcgtat ttgacgatta aggctgagaa caggccagga atcaagccga
     1501 tgataccgtt gcctaaatcc caccaaatcc agaagccata accgctgagc atgtcagcaa
     1561 ggatgttgcc cacgaagccg gtgataaagc cgacgattgg gccaaacgcg ataccaaaga
     1621 acatggggat gacgatagcc gggcgaatac caacgttacc agctccgggg atctgccaaa
     1681 cgttaaaagc ccaggttaaa accgcataaa gtgcggcacc gatagcggca tagacgacct
     1741 gtttggtccc aaatgcc
//
LOCUS       DAOSFC010000278         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_69083_length_1756_cov_2.677249, whole genome shotgun sequence.
ACCESSION   DAOSFC010000278 DAOSFC010000000
VERSION     DAOSFC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69083_length_1756_cov_2.677249"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK791_06455"
     CDS             <1..480
                     /locus_tag="PK791_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92988.1"
                     /translation="VPLIRLVMNYGIHSEAQLLSEYIELRKHLELSYWDDAFEVLKDE
                     QMDYMQFLVDIAFRKLQRKPAIIPTEEQSEFHLTLFRPLGNKVLISILEAAWAMEEDN
                     GGKIIADLDEMLFSWRTRQFIVDALREKDRSKGKNALESLLALPNKPKEAEIITRFE"
     gene            496..>1756
                     /locus_tag="PK791_06460"
     CDS             496..>1756
                     /locus_tag="PK791_06460"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH92989.1"
                     /translation="MAVTELQSKSNLATVVNNFCMTVCTVNGSGSVTANNIIYRALFQ
                     MGIPVSGKNIFPSNIMGMATWFVIRVNDEGFTGRVPYDDIVVAMNPSTLLYETSFLPE
                     GGVIFYADHIMIPEGHRKDLIYYPMPIKELMKTVDVPRNLVSYMENMLYVGIVSQVLG
                     IELSELRRAVDHQFAKRVAVAESNFDIIKIGYEWAQANIEKRDRFVVERRPSKGNLIM
                     TDGNTAAGIGSLFGGLQFCSWYPITPATSMVEAAIAKVGELRKDPVTGKTTCVILQVE
                     DELAAAGAALGAGWAGLRSMTSTSGPGICLMAENIGLAYFTETPMVIWNVQRVGPSTG
                     LPTRTAQGDLSFMYNIGHGDVNHVILMPGTVNECFEFAWKALDLAERYQTPIFVLSDL
                     DLGMNTWVTEKFEYPNTPIDRGKLIWEE"
BASE COUNT          490 a          345 c          436 g          485 t
ORIGIN      
        1 gtgccactca ttcgtttagt catgaactat ggcattcatt ccgaagcgca attactaagc
       61 gaatatattg aattgagaaa acatctggaa ctaagttact gggatgatgc ctttgaggtt
      121 ctcaaagatg aacagatgga ctatatgcaa tttctggttg atatcgcgtt tcgaaaattg
      181 caacgaaaac cagctataat acctactgaa gaacaaagtg aattccatct gactctgttc
      241 cgtcctcttg gtaataaagt acttatcagc attttagaag ccgcatgggc aatggaagaa
      301 gataacggtg gaaaaattat cgctgacctt gatgaaatgc tcttcagttg gagaacacgt
      361 cagttcattg tggatgcatt gcgagagaaa gatagatcta aagggaaaaa tgctcttgaa
      421 agcttgttgg cattaccgaa caagccaaag gaagctgaaa taattacaag attcgaatga
      481 aggagataac caggaatggc agtgactgaa cttcaatcta aatcgaacct ggcgacggtt
      541 gtcaataact tttgtatgac cgtttgtacc gtcaatggtt caggcagcgt aacggcgaat
      601 aatattattt accgtgcgtt gttccaaatg ggaatacctg tctcaggaaa gaatattttc
      661 ccatccaata ttatgggcat ggcgacatgg ttcgtcattc gtgtgaacga tgaaggtttt
      721 actgggcgcg ttccatatga tgatattgtc gtggcgatga atccaagcac cttgttgtac
      781 gaaacatcgt ttttgcccga aggtggggtc attttctatg cggaccacat catgattccc
      841 gagggtcatc gtaaggacct tatttattat ccaatgccta tcaaggaact gatgaaaacg
      901 gttgatgtac cacgaaacct tgtgtcttac atggagaata tgctctatgt gggcattgtc
      961 tcacaggttt tgggcatcga attatccgaa ttacgacgcg ctgtagatca ccaatttgcc
     1021 aagcgtgtcg cggttgcgga gtctaacttt gatattatca agattggata tgaatgggcg
     1081 caggcgaata ttgaaaaacg agaccgcttt gttgttgaac gacgaccaag taagggaaat
     1141 ctcatcatga ctgatggtaa tactgcagct gggattggct ctctttttgg tggattgcag
     1201 ttttgctcat ggtatcccat tacacctgcc acaagcatgg ttgaagcagc gattgccaaa
     1261 gttggtgagc tgcgcaaaga cccagtaact ggaaagacca cctgtgtgat tttgcaggtt
     1321 gaagatgaat tggcagccgc tggtgccgcg ttaggcgcgg gatgggcagg cttgaggtca
     1381 atgacttcta catcgggtcc aggcatttgt ttgatggcag aaaatattgg cttggcttat
     1441 tttaccgaaa cgcctatggt gatctggaat gtgcaacgtg taggtcccag cactggtttg
     1501 cccacccgaa ccgcgcaagg cgatttgtct tttatgtaca acattggaca tggtgatgtc
     1561 aaccatgtca ttttaatgcc tggtactgtg aacgaatgct tcgagtttgc ctggaaagcg
     1621 ctcgatctgg ctgaacgtta ccaaacaccc atctttgtgc tcagtgacct tgatctgggg
     1681 atgaacacct gggtgacaga aaaattcgaa tatccaaaca cacccattga ccgaggcaag
     1741 ttgatttggg aagagg
//
LOCUS       DAOSFC010000279         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_69281_length_1752_cov_4.208603, whole genome shotgun sequence.
ACCESSION   DAOSFC010000279 DAOSFC010000000
VERSION     DAOSFC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69281_length_1752_cov_4.208603"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PK791_06465"
     CDS             <1..1009
                     /locus_tag="PK791_06465"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOH92990.1"
                     /translation="TRGGIVESIHFGAFAVVDPEGNLLVSGGDPNLVTFPRSSMKPFQ
                     VLPFIEAGGQEHFGLTDEELAIMCASHTGTDEHVRVIKSIHEKVGLCEDNLQCGFHWP
                     SDKDTSFEMRKRGETPTPYRHNCSGKHSGMLAMAKMQGEPLDSYLDIKHPVQKRILQK
                     VAEFCEVLPESLIIGTDGCSAPVFAMPLKAFAHATAKLANPESLSESQAKACLTATKV
                     MRAYPTMVAGPGQLDTVLMEVMAGKVFSKGGAEGYQMIGVLPGVLEKYPKGLGITMKF
                     SDGDAGRRATSALTVAILSALGFEKEMQSEAFAKFNAPVLHNSRGLEIGEIKVNQTIN
                     L"
     gene            1012..1695
                     /locus_tag="PK791_06470"
     CDS             1012..1695
                     /locus_tag="PK791_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92991.1"
                     /translation="MLVITPEYQDRLDRVEEILDAYYGIPDWHEYLPAVDELVCTILS
                     QNTNDINRDKAFVKLKETFSSWEEVRDADPEAVKEAIRIAGLANQKGPNIQAALQFIT
                     QERGEIDLDWLKEKTPEETMNWLTQMKGVGLKTASIVMVFSLDMPAFPVDTHVYRVTG
                     RLALRPEKMTIDNAHRWFMSSNRPESFGSLHLNIISLGRDFCQARKPKCGACPVEKLC
                     QYNEKNFDA"
BASE COUNT          493 a          364 c          459 g          436 t
ORIGIN      
        1 gacccgcggg gggattgttg agtcaattca ttttggcgct tttgcagtcg tagaccctga
       61 agggaatttg cttgtgtcag gcggggaccc aaacctggtt actttcccgc gcagttcaat
      121 gaaacccttc caggtattgc cttttattga agctggtgga caagaacatt ttggattgac
      181 cgacgaagaa ctggctatta tgtgcgcatc gcatacaggt acggatgaac atgtgcgcgt
      241 gattaaaagc attcacgaaa aagttggact ttgtgaggat aacctgcaat gtggatttca
      301 ctggccatcg gataaagata ccagttttga aatgcgcaag cgcggagaaa cgccaacgcc
      361 gtatcggcat aactgttcgg gcaagcacag cggtatgttg gccatggcaa aaatgcaagg
      421 cgaaccactg gacagctatt tggatatcaa acatcccgta caaaaaagga ttctgcaaaa
      481 agtagctgaa ttttgtgaag tgctacccga aagcttgatt atcggaacag atgggtgcag
      541 cgcgcctgta ttcgcgatgc ctttgaaagc cttcgcacat gccactgcaa aactggctaa
      601 tccagaaagt ttgagtgaaa gtcaggccaa agcctgctta acggcaacaa aagttatgcg
      661 cgcttatccc acaatggtgg ctggccccgg gcaactggat acagttctaa tggaagtgat
      721 ggctggcaag gtcttttcaa aaggtggcgc ggaaggctat caaatgattg gtgttttacc
      781 aggtgtgctt gagaaatatc ccaaaggttt gggaatcacg atgaagtttt ctgatggtga
      841 tgcgggccgg cgggcaacat ccgctttgac cgtggccata ttaagcgcac ttggttttga
      901 aaaagaaatg cagtcagaag cttttgcgaa gtttaacgcg ccagttttgc ataactcgcg
      961 tgggcttgaa attggcgaga ttaaggtcaa tcagaccata aacctgtaaa aatgcttgtg
     1021 ataacgcctg aatatcaaga ccgtcttgat cgcgtggaag aaatactgga cgcctattat
     1081 ggcatccctg actggcatga gtatttacct gctgttgatg aactcgtgtg cacgattctt
     1141 tcgcagaaca cgaacgatat caatcgcgat aaagcttttg tgaaactcaa ggaaacgttt
     1201 tcaagttggg aagaagttcg agatgcagac cccgaggcgg taaaagaggc gattcgcatc
     1261 gcgggcttag ccaaccaaaa agggccgaat attcaagctg ctttgcaatt tatcactcag
     1321 gaacgtggtg agattgattt ggactggctg aaggaaaaga caccggaaga aaccatgaac
     1381 tggctgaccc aaatgaaagg tgtaggatta aagacagctt ctattgtgat ggtcttttcc
     1441 ttggatatgc cagcatttcc ggtggatacc catgtttatc gcgttacagg gcgtttggct
     1501 ttgcgaccgg aaaagatgac cattgacaat gcgcatcgct ggtttatgtc gagtaaccga
     1561 cctgaaagct ttggcagctt gcatctcaat attatcagct taggacgtga tttttgccag
     1621 gcacgcaagc caaaatgcgg tgcgtgccct gttgaaaagc tgtgtcaata taacgaaaag
     1681 aatttcgacg cctgaaaacc gcttgtcttt agttagtcgg aaaaaactaa gacaaagtgc
     1741 ccaagagtga cc
//
LOCUS       DAOSFC010000280         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_69870_length_1742_cov_3.917012, whole genome shotgun sequence.
ACCESSION   DAOSFC010000280 DAOSFC010000000
VERSION     DAOSFC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69870_length_1742_cov_3.917012"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PK791_06475"
     CDS             complement(<1..360)
                     /locus_tag="PK791_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH92992.1"
                     /translation="MENKKKIAIIGAGFAGLSAAWDLIHAGHDVTLFEASHQAGGLAA
                     GFKEPHWDWSVEHFYHHWFEGDKALRGLLDELGFSDRLIYPKPVSVMYHKGKFYPFDS
                     IPAAIKYPGLGFGLTKIR"
     gene            complement(350..1447)
                     /locus_tag="PK791_06480"
     CDS             complement(350..1447)
                     /locus_tag="PK791_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOH92993.1"
                     /translation="MKIKRWQIYLGVLISAVFLFLAFRKVDFAMVWFHLKSAKWVWVV
                     LGLVLYFLGVIVRAWRWKILLNPLKSISVKKLFPVVCVGYMGNNIYPARAGEFLRAFI
                     LKQKDDVSFSGSLASIVVERLFDGITILALVLFNLGRLVRLITDPVMSRNIQLATWVV
                     SAVFGVLLIVFLLMAIFPQKARTVASWLTQHLLPKKWRAKADGIIDKFIEGVSVLRSW
                     HQVLLVLALSFLVWVLEAGLYFGVMKALGLNLAFIDLLLVEGVVNLVLLVPAMPGGLG
                     TFDAATKFMLELFQQGAEQALGFALILRVALWLPITALGAFYFVKEGFSLSTDIGQLK
                     TDYQEETPVGPENEPSLVTEETKEQDEHNGK"
BASE COUNT          525 a          468 c          347 g          402 t
ORIGIN      
        1 gcgaatcttg gtcaagccaa agcccagccc cgggtatttt atggctgctg gaattgaatc
       61 aaagggataa aacttgccct tgtgatacat cacgctgact ggttttggat aaatgaggcg
      121 gtcgctgaaa ccgagttcgt caagcaaacc acgcaaagcc ttatcgcctt caaaccaatg
      181 gtgataaaaa tgttccacag accagtccca atgtggctct ttaaaacctg cagccaaacc
      241 tccagcctga tggctggcct caaaaagggt cacatcatgc ccggcatgaa tcaaatccca
      301 cgccgcgctt agtcctgcaa aacccgcgcc gatgatagcg attttctttt tattttccat
      361 tgtgttcatc ctgttccttt gtttcttccg tcaccagcga tggttcattt tcgggaccaa
      421 ccggtgtttc ttcctgataa tctgttttaa gttgtccaat atccgtgcta aggctaaaac
      481 cttccttcac aaaatagaaa gcgcccagag cggtaatcgg cagccataac gccacgcgta
      541 aaataagcgc gaatcccaaa gcttgctccg cgccctgctg gaaaagctcc aacataaatt
      601 ttgtcgcggc atcaaacgtg cccaagccac caggcattgc cggcacaagc aagaccaaat
      661 taaccacgcc ttcaaccaaa agcaggtcaa taaaggccag gttcaggcct agggccttca
      721 tcacgccaaa gtataagcca gcttcaagca cccaaaccaa aaacgaaagc gccagcacta
      781 aaagtacctg atgccatgag cgcaaaacgc tgacaccctc gataaatttg tcaatgatgc
      841 catccgcttt ggcgcgccat tttttgggca acaggtgttg ggtcagccag cttgcaacgg
      901 tgcgtgcttt ctgcgggaaa atagccatca gcagaaaaac gatcagaagg acgccaaaca
      961 cagcgcttac gacccaggtt gcgagctgga tatttcgcga catgaccggg tcagttatca
     1021 gacgcaccaa tcgccccaaa ttgaacagta ccagcgcgag aatggtgatg ccatcaaaaa
     1081 gcctttcaac gacgatactc gccagcgagc cggaaaagct gacgtcatct ttttgtttca
     1141 aaatgaaagc gcgcaaaaat tcaccagcgc gtgcgggata gatgttatta cccatgtaac
     1201 ccacacaaac cactggaaac agctttttca ccgaaataga cttcaaaggg ttgagtaata
     1261 ttttccagcg ccaggcacga acgataacac caagaaagta caaaaccagt ccgagaacaa
     1321 cccaaaccca cttcgcgctt ttaagatgga accaaaccat ggcaaaatca actttcctga
     1381 atgccaaaaa gagaaacacg gcgctaatca gcacccctaa ataaatctgc cagcgtttaa
     1441 ttttcatgtc tcgattgtac catcacatgc caggtgcttg aaacccttgc gaagcttaca
     1501 agacaagtca tccataattt acacatcaaa acaaaaatcc tctgtatgtt gtataatagt
     1561 tgatacttca acttttattt tagaatcatt tactgaagga aattgcatta tgaaccaaaa
     1621 gaacccctat cccgattata tctttggtgg catcaattac cccacaaaag gcgcgcggta
     1681 tcatcgtgag catgccttgg gtatccatca tttttctttt catcaaccca accccgctgt
     1741 ta
//
LOCUS       DAOSFC010000281         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_72203_length_1704_cov_4.951486, whole genome shotgun sequence.
ACCESSION   DAOSFC010000281 DAOSFC010000000
VERSION     DAOSFC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72203_length_1704_cov_4.951486"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PK791_06485"
     CDS             complement(<1..536)
                     /locus_tag="PK791_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH92994.1"
                     /translation="MKHFRKLFSITAILLVFALLLGACTKPAPVETPVDAPVEEPVEA
                     IVDGIDCTGVKEGDVVSMLYQWSGEEEAKLTEILAPWMEACKASVSPESSRDQALLDT
                     RVQAGTPPDVAFWQVPQLTQYKDKLVPMDTLGADKSNYAGYWGDIGTIEGKWLGLPVK
                     ADPKTLIWYSPAAFEAAG"
     gene            complement(600..1637)
                     /locus_tag="PK791_06490"
     CDS             complement(600..1637)
                     /locus_tag="PK791_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH92995.1"
                     /translation="MKKKVTSQDVADMAGVSRTTVSFVLNNVKRYAIHPETAARVRKA
                     ARELNYVPNASARALASQQATAIGLMMTRSPEYIATDPFLPQILGGLMDVAKQNKLGL
                     LVEWVEPGQELNTYLKLARARQIDGFIIMTPREDDTGLKALVEAEVPAVVMGYVPGLD
                     MHSVDVDNVQAAKTAVEHLIELGHECIACISNAAKPYTSANQRYEGYKAALTDAGIAF
                     DERLVRFADFSSQSGYDCMKALLEENADFTGVFVASDSVAFGAMAAIRDAGLRIPEDI
                     SIVGFDDIPMASYVTPGLTTIQLPAKEIAEQSCHLLMQLMQGDFCQERIVTLPTQLIV
                     RQSTARPERRP"
BASE COUNT          413 a          427 c          425 g          439 t
ORIGIN      
        1 tagccagcag cttcaaatgc ggcggggctg taccaaatca aggttttggg gtcagctttg
       61 acgggcagac cgagccattt gccttcaatg gtaccgatgt cgccccagta gccggcataa
      121 ttgcttttgt cagcgccaag ggtgtccatg gggacgagtt tgtctttgta ttgagtcagt
      181 tggggaacct gccaaaaagc gacatctggc ggtgtgccag cttgcacgcg ggtgtcgagc
      241 agggcctggt cgcgggaact ttcggggcta acagaagcct tacaagcttc catccagggt
      301 gccagaattt cggtgagttt ggcttcttct tcaccagacc actggtaaag catcgaaaca
      361 acatcgcctt cctttacgcc agtgcaatcg atgccatcaa caatggcttc cacaggttct
      421 tcaaccggtg catccactgg agtttccact ggtgcaggct ttgtacatgc gccaagcaaa
      481 agggcgaata ccagcaaaat tgctgtaatt gagaaaagtt tacggaaatg tttcattact
      541 actcctttat atattatttg ttttttagtt aagtttgatg tggcgtgttt caactcttct
      601 tatggcctcc tttcgggacg agccgtcgat tgacggacaa taagttgggt cggcaaggtg
      661 acaattcgtt cctgacaaaa atcaccttgc atcaattgca tcagcagatg gcaggattgt
      721 tctgcaatct ctttcgcggg caattgaatc gtagttaaac caggcgtaac atatgacgcc
      781 atgggaatat catcaaaacc aacaatggaa atatcttccg ggattctcag tccggcatcg
      841 cgaatagcgg ccatcgcgcc aaaggcaaca ctgtcgctgg ctacgaaaac gccagtaaag
      901 tcagcgtttt cttccaaaag ggccttcatg cagtcgtaac cagactggct gctaaaatcc
      961 gcgaaacgca ccagcctttc atcaaaagcg ataccggcat ccgtcagcgc ggctttgtaa
     1021 ccttcgtagc gttgatttgc tgaggtataa ggcttggcag cgttgctgat acaggcaatg
     1081 cactcgtgcc ccagttcaat gaggtgttca accgcggtct tggccgcctg cacgttgtca
     1141 acatcaaccg aatgcatgtc caggcctggg acatagccca tcacaacagc cgggacttcc
     1201 gcttccacga gcgctttcag accggtgtca tcttcgcgtg gggtcataat aataaaacca
     1261 tcaatctggc gcgcgcgggc aagtttcaga taggtgttta gttcctgtcc aggctcaacc
     1321 cactcaacga gcaatcccag tttgttttgt ttcgcaacgt ccatcaatcc gcccaaaatt
     1381 tggggcagaa aagggtcagt tgcgatgtat tcagggctgc gggtcatcat aaggccaatc
     1441 gctgttgcct gttgtgatgc caaagcgcgg gctgaggcat taggcacgta gtttaattcc
     1501 cgggctgctt tgcgaacccg cgcggcagtt tcgggatgga ttgcataacg tttgacgtta
     1561 ttcaaaacga aagagactgt cgttctcgat acgcctgcca tgtcggcaac gtcctgactt
     1621 gtaactttct ttttcatacg gactcctatc tgaattaagg aacaaaataa cttttacaca
     1681 gcctattaac gcgcgttaat agtt
//
LOCUS       DAOSFC010000282         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_72914_length_1693_cov_4.158120, whole genome shotgun sequence.
ACCESSION   DAOSFC010000282 DAOSFC010000000
VERSION     DAOSFC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72914_length_1693_cov_4.158120"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PK791_06495"
     CDS             <1..628
                     /locus_tag="PK791_06495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH92996.1"
                     /translation="YAKQSLKTADMKAYQDSLILTFIKQNIKPAAKILEIGGGQSRVI
                     QLLHENYSFWNLDKLEGAGFGPKRPYDAHGHVLVQDYLGAFSPELEDHSFDFIYSIST
                     IEHLPHDEETLTRCLADMNRLLAPGGVCLHCVDALLKPDKLEVHPLVPKVLSENRTAR
                     TNLCFDTLSKDPDLWSLPFYAYYTRYFHLTKKRLKPFGKPITINVYWE"
     gene            840..1331
                     /locus_tag="PK791_06500"
     CDS             840..1331
                     /locus_tag="PK791_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOH92997.1"
                     /translation="MRKAELNEFYDEWIQAIYRYTLSRVKHVDDAQDITSQTFLSAWQ
                     NRERYQERGEFAAWLFSIARNKIKDHFRQAQRKQNRIQTLGDDEIYKLSAEPQHETLI
                     ALRQVISTLSEKDQELIRLRYVAGLDFATIAKISRRSPAACRKACSRLVAKLKAHFEQ
                     EQE"
     gene            1340..>1693
                     /locus_tag="PK791_06505"
     CDS             1340..>1693
                     /locus_tag="PK791_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH92998.1"
                     /translation="MTKLNTNSLNPFEEKISLAIQVKDAPEDFTQTLKSQLESIPESK
                     EMAQNLRLKSPKMRWAVAGISLALIAVIVVFAIGPKKVWAEISSFLQRLPGVGNVEDL
                     GESCVLAKPISITQEG"
BASE COUNT          505 a          377 c          371 g          440 t
ORIGIN      
        1 gtatgccaaa caatccctga aaaccgctga tatgaaagct tatcaggact ctttaatctt
       61 aacttttatc aaacagaata ttaaacctgc tgcaaagata cttgagattg gtggggggca
      121 atctcgtgtt atccaactgt tgcatgaaaa ctactctttc tggaatcttg acaagctgga
      181 aggcgcaggc tttggcccca aaaggcccta cgatgcccac ggacatgtgt tggttcaaga
      241 ttatttgggc gcattcagtc cagaacttga agaccattcc ttcgatttca tctatagcat
      301 ttcaaccatt gaacatctgc cacatgatga ggaaacattg acacgttgcc tggctgacat
      361 gaaccgcctg cttgctccag gtggagtttg tttgcattgt gttgacgcac ttttgaagcc
      421 ggataaactt gaagtgcatc cgcttgttcc aaaagtgctt tcagaaaaca ggactgccag
      481 aacaaacttg tgttttgata cgctctcaaa agatcctgat ttatggagtt tgccctttta
      541 cgcttattac actcgctatt ttcatctgac aaaaaagaga cttaaacctt tcggtaagcc
      601 gattacaatc aatgtttatt gggaataaga cgagacataa ttcttcaaca gaattaaatt
      661 ctatcttttt cattagtgtt tagaattatt aatatttatt ccagaggaat ctttcatctg
      721 gtttttatcg tgtttaaaag tcaaagggtt ctttgccttg aaaaagtggt tcaacattac
      781 tgtcacaaaa agcacttgaa tgacgttatc cttaacaaag cgcgcgaaga tggaggttta
      841 tgcgaaaagc tgaactgaat gaattttacg atgaatggat ccaagcgatt tatcgctata
      901 cgctctcaag agtaaagcat gtggatgatg cccaagacat taccagtcaa acctttctct
      961 ctgcctggca aaatcgtgaa cggtatcagg aacgtggcga gttcgccgcc tggctcttta
     1021 gcatcgcgcg caataaaatc aaagatcatt tcaggcaagc ccaacgaaag cagaatcgca
     1081 tccaaacgct gggcgatgat gaaatttaca aactatccgc tgaaccgcag cacgaaacgc
     1141 ttatcgcttt gcgccaggtc atcagcactt tatcagaaaa agaccaagaa ctaattcgcc
     1201 tgcgctatgt ggcagggctg gattttgcca ccatcgctaa aatcagccgt cgcagtccag
     1261 cggcgtgccg aaaagcctgc agtcgcctgg tggcgaagct aaaagctcat tttgaacaag
     1321 agcaagaata gaggacgaaa tgacaaagct taatacgaat tccttaaatc cctttgaaga
     1381 aaaaatttcc ctggcaatac aggtgaaaga cgcacccgaa gacttcacac aaactttaaa
     1441 atcacaactg gaaagtattc cggaaagcaa ggagatggcg caaaacttaa ggctgaaatc
     1501 tccaaaaatg cgctgggcgg tggcaggcat cagcctggca ctcatcgccg tcatcgtcgt
     1561 ctttgccatc ggtccaaaaa aagtttgggc ggagatatcc agtttcttac aacgcttgcc
     1621 tggcgtgggc aacgtggaag acctcggtga aagctgcgtg ctggcgaagc cgattagcat
     1681 tacccaggag ggt
//
LOCUS       DAOSFC010000283         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_73842_length_1679_cov_5.121305, whole genome shotgun sequence.
ACCESSION   DAOSFC010000283 DAOSFC010000000
VERSION     DAOSFC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73842_length_1679_cov_5.121305"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PK791_06510"
     CDS             <1..897
                     /locus_tag="PK791_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOH92999.1"
                     /translation="KSTLDDAELAPFQTFTDLDKALAQKPDGVIIANPSALHMGVALP
                     AARAGANLLIEKPVSDTLDGLAELQETLDKHGKRAMTGFHFRYHPVLQDIKRVIQAGT
                     LGKVYYAKAHWGEYLPDWHPWEDYRQTYAARADLGGGVLNTLSHPFDYLRWLLGEVET
                     LGAQLAHLSALELSVEDHADISLKFASGAMASVHLDYYQKPDSHTLDITFEKGRIAWD
                     NATSSAVMHYADGRPSETLLAPEGFERNTMYLEEMRAFLSLCRGETLDYCSLQDGIRA
                     QQIVMTAKRSNAAGGMLLAVEW"
     gene            908..>1679
                     /locus_tag="PK791_06515"
     CDS             908..>1679
                     /locus_tag="PK791_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH93000.1"
                     /translation="MTERTVLDKFNMRGKTALVTGGAGLLGKRFSLALAQAGANVVVA
                     DLNLNAAQAHAETLVSKGYSAIALALDVTDPASCKQVVAQTLERFGGIDALVNSAGMD
                     PKFDPQNLDRQNKNAFENYDLEAWRFTLEINLTGMFLATQATVPAMLEAGKGSIVNIS
                     STYGLNGPDQRIYPQIDGQISYKPVDYSVAKAGVIGFTRYLAAYYAGKNIRVNALSPG
                     GIFNGHNDDFSRNYAAKTIMGRMADIDEMDAAMLFLCSD"
BASE COUNT          400 a          444 c          444 g          391 t
ORIGIN      
        1 aagtccactt tggacgatgc cgagctggcg ccatttcaaa cttttactga tttagataaa
       61 gcactcgctc aaaaacctga cggtgtaatc attgcgaacc ccagcgcctt gcatatgggc
      121 gtggcattgc ctgccgcgcg tgccggggcg aatcttttaa tcgaaaaacc ggtatcggac
      181 accttggacg gtctggctga attacaagag acactggata agcacggcaa acgtgccatg
      241 accggcttcc attttcgcta tcatcctgtc ctgcaagaca tcaagcgcgt catccaagcc
      301 ggcaccttgg gcaaagtcta ctatgccaaa gcccactggg gagagtacct gcccgactgg
      361 catccctggg aagactatcg ccaaacctac gccgcgcgcg ctgatttggg tggcggggtg
      421 ctgaacacgc tttcgcaccc ctttgactac ctgcgttggc tgttgggcga ggtggaaaca
      481 ctcggcgcgc agcttgcgca tctaagcgcg cttgaattga gcgtggaaga ccatgccgat
      541 atcagcctga agttcgccag cggcgcgatg gcgagtgtgc atcttgacta ctatcaaaaa
      601 cctgattccc ataccctgga tatcaccttt gaaaaagggc gtatcgcgtg ggataatgca
      661 acgtccagcg cggtgatgca ttacgccgat ggacgcccgt ccgaaacgct tttggctccc
      721 gaaggctttg agcgcaacac aatgtatctt gaagaaatgc gcgctttttt gagcctgtgc
      781 cgtggcgaga cgctggatta ctgcagcctg caagatggca tcagagcgca gcaaattgtt
      841 atgaccgcca aacgttcaaa cgctgctggt ggcatgcttt tggcagtaga gtggtagaat
      901 gtgactcatg actgaaagaa cagttcttga caaattcaat atgcgcggaa aaaccgcact
      961 ggtaaccggc ggagcgggtt tgcttggcaa acgcttttcg ctggctttag cgcaagccgg
     1021 agccaacgtg gttgtggctg atttgaattt gaatgccgcc caggcccacg ccgagacctt
     1081 ggtcagtaaa gggtattctg ccatcgcgct cgctttggac gttaccgacc ccgcatcatg
     1141 caaacaagtt gtcgcccaaa ctttggaacg ttttggtggc attgacgctt tggtaaacag
     1201 cgccggcatg gaccctaaat tcgacccgca aaacctggac aggcaaaaca aaaacgcttt
     1261 tgaaaactac gacttggaag cctggcgctt tacgctcgaa atcaatctca ccggtatgtt
     1321 cctggcaacc caagccactg taccggcgat gcttgaggcg ggcaagggca gtatcgttaa
     1381 tatcagttcg acttacggtc ttaatggccc cgatcagcgc atttatccgc agattgacgg
     1441 gcagatttct tataaacccg tggattattc tgttgctaaa gccggagtaa tcggctttac
     1501 acgctatttg gctgcttact acgccggaaa gaacattcgc gtgaacgccc tttctcccgg
     1561 tggcattttc aacggccaca atgatgactt ttcacgcaac tacgctgcga aaacaatcat
     1621 gggacgcatg gcagacattg atgagatgga cgccgcgatg ctctttttgt gttccgatg
//
LOCUS       DAOSFC010000284         1656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_75511_length_1656_cov_2.855091, whole genome shotgun sequence.
ACCESSION   DAOSFC010000284 DAOSFC010000000
VERSION     DAOSFC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75511_length_1656_cov_2.855091"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PK791_06520"
     CDS             complement(<1..300)
                     /locus_tag="PK791_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH93001.1"
                     /translation="MMENKKKIAIIGAGFAGLSAAWDLAQAGHDVTVFEFSHQAGGLA
                     AGFKEPHWDWSVEHFYHHWFEGDKALRALIDELGFSDRLIYPKPVSVMYHKGKFYP"
     gene            complement(287..1384)
                     /locus_tag="PK791_06525"
     CDS             complement(287..1384)
                     /locus_tag="PK791_06525"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOH93002.1"
                     /translation="MKIKRWHIWLGVLISALFLYLAFRKVDFGLVWFHLKSAKWAWVL
                     LGLVFYFAGVVVRAWRWKVLLNPLKPMSVKKLFPVVCVGYMGNNIYPARAGEFLRAFI
                     LKQKDDVSFSGSLATIVVERLFDGITILALVLFNLGRLVRLVTNPVMANNIRIATWIV
                     GGGFGVLLIVFLLMAIFPKKTRIVSSWLTRHLLPKKWRAKADGMIDKFIEGVSVLRSW
                     HQILLVLALSFLVWVLEAGLYFGVMKALGLNLTFIDLLLVEGVVNLVLLVPAMPGGLG
                     TFDAATKFMLELFKQGAEQALGFALILRVALWLPITALGAYYFVKEGFSLSTDIGQLK
                     KEYQEETQMDPEDDNSQMTEEIEEHNEHDGK"
BASE COUNT          522 a          451 c          293 g          390 t
ORIGIN      
        1 gggataaaac ttgcccttgt gatacatcac actgaccggt tttggataga tgagacggtc
       61 gctaaaaccg agttcgtcaa tcaaagcacg caaggcttta tcgccttcaa accaatggtg
      121 gtaaaaatgt tccacagacc agtcccaatg tggctcttta aagccggctg ccaagccacc
      181 tgcctgatga ctgaactcaa aaacagtcac atcatgcccg gcctgagcta aatcccatgc
      241 cgcgctaagc ccggcaaagc cagcaccgat gatagcaatt ttctttttat tttccatcat
      301 gttcattgtg ttcctcaatt tcttccgtca tctgagaatt atcatcctct ggatccatct
      361 gtgtttcttc ctgatactct tttttaagct gtccgatatc cgtactcaga ctaaagcctt
      421 ctttcacaaa ataataggct cccagagcag tgattggcaa ccacaacgcc acgcgcaaaa
      481 tgagcgcgaa acccaaggcc tgctccgcgc cctgcttgaa aagttcaagc ataaatttgg
      541 tcgccgcatc gaaagtaccc agtccgccag gcatagccgg cacgagcaaa accaaattca
      601 ccacaccttc aaccaagagc aaatcaataa aagttaggtt caggcccaga gctttcatca
      661 caccaaagta caggccagcc tcaagcaccc aaactaaaaa cgaaagtgcc agcaccaaca
      721 aaatctgatg ccacgaacgc aacacgctaa caccctcgat aaatttatca atcatgccat
      781 ccgctttggc acgccatttt ttgggtagca gatgtcgcgt tagccagctt gaaacaatgc
      841 gcgttttttt ggggaagatt gccatcagca gaaacacaat taaaagaacc ccaaatccac
      901 ccccaactat ccaggtcgcg attcgaatat tattagccat gaccggattg gtgaccaggc
      961 gtaccaaacg gcccaaattg aacaatacca gagccaaaat ggttatccca tcaaaaagtc
     1021 tttcaaccac aatggttgcc aatgagcctg aaaaactgac atcatctttt tgtttcaaga
     1081 tgaaagcgcg caaaaattca cctgcacgcg cgggataaat attgttaccc atatacccca
     1141 cgcagaccac aggaaaaagt tttttcacag acattggttt gaggggattg agtaagactt
     1201 tccaacgcca ggcgcgcaca accacacctg caaaataaaa aaccaagccc aacaaaaccc
     1261 aggcccactt cgcgctttta aggtggaacc aaaccaaacc aaaatccact tttctgaacg
     1321 ctaaataaag aaaaagagcg ctaatcagca cccccaacca aatatgccaa cgtttaattt
     1381 tcataccccg attgtaccat tacaaggcat ttcattgaaa tttgaacatt ttctattctt
     1441 tgttcaaaca ctttgaaaaa tcagataaat gatgtaaaat agttgacaca tcaactttat
     1501 ggtttttgcc aaaggaatac tgcatgagtc caaaacaccc ctatcccgat aacatctacg
     1561 gtggtatcaa ccaccctaca aaaggcgcgt ggtatcatcg cgagcatgcc ttgggtatcc
     1621 atcatttttc ttttcatcaa cccaaccccg ctgtta
//
LOCUS       DAOSFC010000285         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_75815_length_1652_cov_2.607389, whole genome shotgun sequence.
ACCESSION   DAOSFC010000285 DAOSFC010000000
VERSION     DAOSFC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75815_length_1652_cov_2.607389"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PK791_06530"
     CDS             complement(<1..794)
                     /locus_tag="PK791_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH93003.1"
                     /translation="MKNKAHLVATLQALLVTFLWSTSLIIIKLFINEIPPLLFAGLRY
                     TMAVIILAPVLWRYREDIKSLSKRDWLRLFILGVVYYFITQGAQFISLKYVDMVTISL
                     ILTFTSPLVAYIGIIFLKEPVLPQQWAGTILFIIGALIYFSQKQLSSMSVLGLGMVLL
                     SLLTNSAGTVLGRSANRDRKLNALVVTGISMFFGAVLLLGSGLSLHGMPKLALKSWLI
                     IFYTASINTAFAFWLWNHSMRTLSAMESSVINNTMLIQISVLSWIIL"
     gene            complement(1024..1650)
                     /locus_tag="PK791_06535"
     CDS             complement(1024..1650)
                     /locus_tag="PK791_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH93004.1"
                     /translation="MVRLGLKSFFATTPDIEVAGEASNGYEAIQVAQDLLPDVILMDL
                     LMPGMDGVEATRQIKAKLPGIEVIVLTSYHEDENIFPAIRAGALSYLLKDIDPDELSD
                     AIRLAARKEAVLNPQVASRLMQEVRGGRRADDYNPLNDLTDREMEVLQEIASGKSNAE
                     IATALFISEKTVKTHITKILSKLHLSDRTQAAVLAWQSGVVKKGDWKR"
BASE COUNT          490 a          378 c          381 g          403 t
ORIGIN      
        1 agaataatcc acgaaagcac actaatttga atgagcatgg tgttgttaat cacgctcgat
       61 tccatcgcgc tgagggtacg catactgtga ttccatagcc aaaaggcaaa agcagtattt
      121 attgaagctg tgtaaaaaat gattaaccat gatttcaaag ctaacttagg catgccatgc
      181 agggaaaggc ccgacccaag caacaaaacc gcgccaaaaa acatgctgat gcccgtcaca
      241 accagggcgt tgagttttcg atcgcgatta gctgagcgac ccaataccgt acccgcggaa
      301 ttggtaagca acgaaagcaa gaccattccc aaacccaaaa cgctcatgct tgaaagttgt
      361 ttttgcgaaa agtaaatcag cgcgccgata ataaaaagaa tggtgccagc ccactgctgt
      421 ggcaggaccg gttctttgag aaagataatc ccaatataag cgaccagagg tgaggtaaag
      481 gtaagaatga ggctgatggt caccatgtca acatatttaa gcgagataaa ctgcgcgccc
      541 tgggtgatga aatagtaaac cacaccaagg atgaaaagcc taagccagtc gcgtttacta
      601 agtgatttga tgtcttcacg gtagcgccac aatacgggag ccaaaataat aacagccata
      661 gtgtagcgca aaccagcaaa aagcaaaggt ggaatctcgt ttatgaaaag cttgataata
      721 ataagcgagg ttgaccacag aaaagtgacc agcaaagcct gcaacgtcgc gacaagatga
      781 gctttatttt tcatgatgct tttattgtaa atgtttgggc aaaaatgtgc gcttttgatg
      841 atgcataaca agagactctt ctaaacaaga agcgacatag cttgatttgc tattaacaca
      901 tagccagcaa aactaaagca caaaagagaa tttttgcgat tttgagttat gtcattttga
      961 agcgtcttct cacactcact tttagctcag gtttagatat cgggcacagc gattaatacc
     1021 ccattacctt ttccaatccc cttttttcac aaccccactt tgccacgcaa ggacggcagc
     1081 ctgggtgcgg tcgctgaggt ggagtttgga cagtatcttg gtgatatgtg tcttaaccgt
     1141 cttttcactg ataaacaagg cagtggcgat ttcagcattg gactttccgc tggcaatttc
     1201 ctgcaacact tccatttcgc gatcggtgag gtcgttgagc gggttatagt catcggcgcg
     1261 ccgtcctccg cgcacttcct gcatcaggcg cgaagccacc tgcggattaa gcacggcttc
     1321 tttgcgcgcg gcaaggcgaa tggcatcgct taattcatct gggtcgatgt cttttaaaag
     1381 atatgatagt gccccggcgc gaatcgcggg aaaaatattt tcatcttcat gataggaagt
     1441 aagcacaata acttcgatgc cgggaagttt cgctttgatt tgacgagtgg cttcaacacc
     1501 gtccatacct ggcatgagca aatccattaa aatcacatcc ggcaaaaggt cctgggccac
     1561 ctgaatggct tcatagccat tagaggcttc gcctgcgact tcaatatccg gtgtggtagc
     1621 aaaaaagctt tttaagccca atcgcaccac gg
//
LOCUS       DAOSFC010000286         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_80346_length_1590_cov_7.087948, whole genome shotgun sequence.
ACCESSION   DAOSFC010000286 DAOSFC010000000
VERSION     DAOSFC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80346_length_1590_cov_7.087948"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PK791_06540"
     CDS             <1..1575
                     /locus_tag="PK791_06540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOH93005.1"
                     /translation="QLPSMQVLNTLDIESVSGQQTTPADTETPTLSPTLNASQLASLT
                     PKPSNTPSPTPNPVFSTGDIENYSVWQVNQEDENASLLSLREIPKTLIPLPDDYKQDA
                     LLKNFLAFIQILNQIRQTNNDTWLDELKNTDWHLEYQKLTDHSNTDFAQTIDTFRIAN
                     AENNVTQVINDAVGLSALYATMDFEGISSQQAQRVCEIIADPTLENKADLLHENKVQF
                     SWFFKSGDYQVGDIAILPGENCGMGLVVGKSDEQLLLVVPDLLKDGQLQSILVDERSI
                     DAVFGEMKYVFPGKKDHSAGSVVAGSMDIFKPGVTTIVNADRYENIQLPFDTMSDRGL
                     FDNNIVITIDDCYNNQHVRAIFELLKNNGLRATFFPNTNYLLFDEETISLWQEIYQAG
                     FEIGYHTTNHATHLSYAELEEDFWAFTQHMRALLEDDSFEINLVRAPYGVWYENWYLW
                     SANNGLMSVDWNVLTEHQPERAELRLQEGISPIVLLHSTAFDVEWLNTNLNQLIMLAR
                     QYDGIVGSVYDCVHRE"
BASE COUNT          453 a          356 c          379 g          402 t
ORIGIN      
        1 cagttgccgt ctatgcaggt tttaaatacc ttagacattg aaagtgtgtc agggcaacaa
       61 acgacacctg ccgatacaga aacgccaaca ttatcgccaa cgctaaacgc cagccagctt
      121 gcgtcgctca cgccaaagcc aagcaacact ccatcgccaa cgccgaaccc tgttttttcg
      181 acaggggata ttgagaatta ctcagtctgg caagttaatc aagaagatga aaatgccagc
      241 ttactcagtt tgcgggaaat acccaagact ctcattccat tgcctgacga ctataaacaa
      301 gatgcactgc tcaagaattt ccttgctttt atccaaattc tgaatcagat caggcaaacg
      361 aataacgata cctggctgga cgaactaaaa aacacagact ggcatcttga atatcaaaaa
      421 ttgactgatc attccaacac agatttcgct caaactatag acacttttag aatagcgaac
      481 gcagaaaaca acgtaacgca agtcatcaat gacgcagtcg gtttgagcgc tttgtatgcg
      541 accatggatt ttgaaggcat cagcagccaa caggcgcaac gcgtttgtga gattatcgct
      601 gacccgacac ttgaaaataa agccgatctg ctgcatgaaa acaaagtcca gtttagctgg
      661 ttctttaaga gtggggatta tcaggtgggg gatattgcca tcctgccagg cgaaaactgt
      721 ggcatggggc tggtagtggg caagtcagat gagcaattgc tgttagtcgt gcccgattta
      781 ttgaaggacg ggcaacttca atccattctg gttgacgaac gtagcatcga cgccgttttt
      841 ggcgagatga aatatgtctt tcccgggaaa aaggaccact ccgctggctc cgttgtggcg
      901 ggctcaatgg acatttttaa gccaggggtg acgaccatag tcaatgcgga tcgttatgag
      961 aatatccagc tgccttttga tacgatgagt gacaggggcc tgtttgacaa caatatcgtt
     1021 atcaccattg atgattgcta caataatcag catgtcaggg cgatatttga gctcttgaaa
     1081 aataacggac tgagagcgac attctttcca aacaccaact atttgttatt cgatgaagaa
     1141 accataagct tatggcaaga aatctatcag gctggttttg aaattggcta tcacacaacg
     1201 aaccatgcca cacatctctc ctatgcggaa cttgaggagg atttttgggc ttttacccaa
     1261 catatgcgcg cgctgctgga agatgatagt tttgaaataa atttggtgcg cgcgccttat
     1321 ggcgtgtggt atgaaaactg gtatctctgg tccgcgaata acgggctgat gagtgtggac
     1381 tggaacgtgc tcaccgaaca tcagcctgaa agggccgaac taaggttaca ggaaggtatt
     1441 agcccgattg ttcttttgca ttcaacggct tttgatgttg agtggcttaa cacgaatctg
     1501 aatcagctca tcatgcttgc ccgtcaatat gatggcatcg tggggagtgt ttacgactgc
     1561 gtacatcgcg aatgagcctg cccgcgctta
//
LOCUS       DAOSFC010000287         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_81387_length_1577_cov_4.883049, whole genome shotgun sequence.
ACCESSION   DAOSFC010000287 DAOSFC010000000
VERSION     DAOSFC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81387_length_1577_cov_4.883049"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PK791_06545"
     CDS             <1..1190
                     /locus_tag="PK791_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH93006.1"
                     /translation="TAAQWGLIMLIYAFIDAADNPIYGYLSDRTRTRWGRRRPWLVIG
                     TPLLVLCLVLFFSPPAWLSANQLFIYAMLFYILTGTLDSVINANYGALFPELFKTDLS
                     RGQANALRQAFQLVAMIISIALTPIVVDAIGWSTTAIIYGILGGAVILYMTFTSHEEH
                     ISIDEPKPKIWDSVKTLLTNRKFWVAGVSNAFYSAAMSMVMAGLPLYAKYKLGIGGWK
                     QALLMGLVLVVAIVSVAIWAAFLKRYDLVKVWRWALLSLAISFVPLYFAQGYVMALIC
                     ASLVGFGFAGVITTMDLIHARVMDEDKAKHNVRREGIISNALGFMNRLNSLFTGGAFL
                     LLKSLYGYESGDVPGSQPDQVALFIIAIFPLIMTLLSYACSYLSDFKVVKSEVTDQSL
                     LDA"
     gene            1214..>1577
                     /locus_tag="PK791_06550"
     CDS             1214..>1577
                     /locus_tag="PK791_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOH93007.1"
                     /translation="MALLQINHKSNAVKTNLVLNISIPDITEEDPRPIHERKVLWLLH
                     GLSADGSAWLRYGNADWVSRELGVIIIMPSGDRSFYVNMDNGQNYFSYLTEELPEWID
                     ARFKFNLNRDNSLIAGLSM"
BASE COUNT          375 a          384 c          383 g          435 t
ORIGIN      
        1 tgacagcggc acagtggggc ttgattatgc tcatttatgc cttcatcgat gctgcggaca
       61 accccattta cggctatctc tcagaccgca cacgcacgcg ttggggtcgc cgtcgtccat
      121 ggttagtcat tggaacgcca cttttagtct tgtgtttggt gctcttcttc tcgccacccg
      181 cctggctgag tgccaatcaa ctctttatct acgccatgct cttttacatc ctgacaggca
      241 ccttagactc ggtaatcaac gcgaattatg gcgcgttatt cccggaactt ttcaaaactg
      301 accttagccg gggtcaggct aacgcgctgc gccaggcatt ccagttggtg gcgatgatta
      361 tcagcatcgc gcttaccccg attgtggtgg atgcgattgg ctggtcaaca acagcaatta
      421 tttacggtat tttgggtggc gcggtaatcc tgtatatgac tttcaccagc cacgaagaac
      481 atatcagcat tgacgagccg aaaccaaaaa tttgggatag cgtgaagacc ctgcttacca
      541 accgcaagtt ttgggtagcc ggggtcagca acgcttttta cagcgcggcc atgtcgatgg
      601 tgatggcagg cttgcccctt tatgccaaat acaaactggg catcggtggc tggaaacaag
      661 ccttgctgat gggactggta ttggtggtgg cgattgtctc ggtggcgatt tgggcagcct
      721 tcctaaaacg ctatgacctg gtgaaagtat ggcgctgggc tttactttca ctcgccattt
      781 cgtttgtgcc gttgtatttc gcccaaggtt acgtcatggc acttatttgc gcctccttgg
      841 ttggctttgg ttttgctggt gtgattacaa cgatggatct catccacgcc cgggttatgg
      901 acgaagacaa agccaaacac aatgtacgcc gtgaaggaat aatcagcaat gccctaggtt
      961 tcatgaaccg tctcaacagt ttatttaccg gcggggcgtt tttattgctg aaatcgctat
     1021 atggctatga atcgggtgat gtacctggat cacaacctga ccaggtggcg ttgtttatca
     1081 tcgctatctt tcccctgata atgaccttac tcagctatgc ttgctcttat ttgagtgatt
     1141 ttaaagttgt aaaaagtgaa gtaacagatc aatccttatt ggatgcataa gaaacacata
     1201 aaaggacgaa aaaatggcat tactacaaat taaccacaaa tcaaacgcag tcaagactaa
     1261 tcttgtgctc aacatctcta tacctgatat caccgaagaa gatccccgcc ccatacacga
     1321 acgtaaagtg ctttggcttt tgcatggact cagcgccgac ggaagcgcat ggcttcgcta
     1381 tggcaacgct gactgggtgt cgcgagagtt gggtgtcatc atcatcatgc ccagtggtga
     1441 ccgcagtttt tacgttaata tggacaacgg ccagaattac tttagttatc tgaccgaaga
     1501 actgcctgaa tggattgacg cccgctttaa gttcaatctc aaccgcgata attcccttat
     1561 cgccgggctt tcaatgg
//
LOCUS       DAOSFC010000288         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_81488_length_1576_cov_3.331361, whole genome shotgun sequence.
ACCESSION   DAOSFC010000288 DAOSFC010000000
VERSION     DAOSFC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81488_length_1576_cov_3.331361"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PK791_06555"
     CDS             complement(<1..392)
                     /locus_tag="PK791_06555"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOH93008.1"
                     /translation="MHALQDPAYRAFITPLIPTVEPERVLGVRTPTLRALAKRLWREE
                     QNGCARFMAELPHFYFEENMLHAFFIEQEKDFHTALAYTQAFLPHIDNWATCDSFSPK
                     VFKKNPEALYPIALAWIQSPHEYTARYG"
     gene            564..1250
                     /locus_tag="PK791_06560"
     CDS             564..1250
                     /locus_tag="PK791_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH93009.1"
                     /translation="MPKTTFYNLSAARRARIETAAINEFAENGLKSAVLNNIVANAGI
                     AKGSFYQYFENIEDLYQHVLTLVQKRKTEMVASLGTPANIMDTFAYLRWLMQLSVIFM
                     LREPKLAKIERRDFVDHPVIKTIDPETGQRMSGDGRFHALLTQGVLHDDLATWVDTDM
                     AAYVLNLIANLVAPYIIDRIHDKGIPLSDSPESVVYDPMVQALFDNLMDIIEAGIARD
                     PQIRQDFYSK"
     gene            complement(1253..1519)
                     /locus_tag="PK791_06565"
     CDS             complement(1253..1519)
                     /locus_tag="PK791_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOH93010.1"
                     /translation="MGYTVEIFDHAFKQLSKLDKPVQKLLLNWLEKNLQGTENPRLKG
                     KGLVGNHSREWRYRVGDYRIIVDIQDKVLIVLIIAVGHRKDIYL"
BASE COUNT          390 a          343 c          409 g          434 t
ORIGIN      
        1 atgccataac gcgcggtgta ttcgtgtggg ctttggatcc aggcaagggc gatggggtag
       61 agcgcttcag ggttcttttt gaagactttg ggcgaaaagc tgtcgcatgt tgcccagtta
      121 tcgatatgag gtaaaaaggc ttgtgtatag gcaagcgcgg tgtggaagtc tttttcctgc
      181 tcaatgaaaa aggcgtgcaa catgttttct tcaaagtaaa agtggggtaa ctctgccatg
      241 aaccgcgcgc agccattttg ctcttcacgc cataggcgtt tggcaagggc gcgcaatgtt
      301 ggagtgcgca cgcctaaaac tctctcgggt tcaacggtgg ggatcaatgg cgtgatgaaa
      361 gcacggtatg cggggtcttg aagagcgtgc aagcttttat cacggttaga acaatcctga
      421 gggttgctga aggggtcatt catcctttta tcttatcgca atcccatcgg tttttgtgag
      481 attgctttat tgaatgtaca acgcgttgac agcaggaata agacgaagta gaatatgacc
      541 gttcagtcat tacaggaaga gctatgccaa aaacaacttt ttacaatcta agcgctgccc
      601 gccgcgcgcg cattgaaaca gcggcaatca atgagtttgc tgagaacggc ttaaaatctg
      661 ccgtgctcaa taatatcgtc gccaatgctg gcattgccaa gggttcgttt tatcagtatt
      721 ttgagaacat tgaggatcta taccagcacg ttctgacact cgttcaaaaa cgcaaaacag
      781 aaatggtcgc atcgcttggc acacctgcca acatcatgga tactttcgcg tacctgcgtt
      841 ggttaatgca gttaagcgtc atttttatgt tgcgcgagcc caagttggcc aagattgaac
      901 ggcgcgattt tgtggatcac ccggtcatta aaacgattga ccctgagacc gggcagcgca
      961 tgagtggaga tgggcgtttt catgccttgc tgacccaggg tgtcttgcat gacgatttgg
     1021 cgacctgggt ggacaccgac atggcagctt atgtcttgaa tttgattgcc aacctggtgg
     1081 cgccctatat tattgaccgc atccacgata aaggcatccc gttaagtgat agcccggaaa
     1141 gtgtcgttta tgaccccatg gtgcaagccc tgtttgataa tttgatggac atcatcgagg
     1201 cgggaatcgc cagagatccg caaatcaggc aggatttcta ctcgaaatag ttttaaagat
     1261 agatgtcttt gcggtgcccc acggcaataa ttaggacgat gagcaccttg tcttgaatat
     1321 cgacaatgat gcgataatcg ccaacacgat atcgccactc tcgtgaatga ttacccacca
     1381 agccttttcc ttttagtctg ggattttcag ttccttgcag gttcttttca agccagttga
     1441 gaagaagctt ttggacaggt ttatccagtt tggaaagctg tttaaaggcg tggtcgaata
     1501 tttctacggt gtagcccatt gttagatgcc aagaagcctg gctgtctctt caaggctata
     1561 gcttttcgga tttttc
//
LOCUS       DAOSFC010000289         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_82063_length_1568_cov_4.561798, whole genome shotgun sequence.
ACCESSION   DAOSFC010000289 DAOSFC010000000
VERSION     DAOSFC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82063_length_1568_cov_4.561798"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK791_06570"
     CDS             <1..220
                     /locus_tag="PK791_06570"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOH93011.1"
                     /translation="QTRQKQAGIAPFSPHDIRRTTITDMLSADVDVLTVSAIAGHASA
                     DTTRRYDKRGDESKREAARKIQSPFKGH"
     gene            complement(231..461)
                     /locus_tag="PK791_06575"
     CDS             complement(231..461)
                     /locus_tag="PK791_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH93012.1"
                     /translation="MDHKPENDEKEFDPNWAKNLMAELKQSFSDVGDSFEKAWNEGKN
                     DPKIKKFGEEMKSTFKKMGEDIEELFSSKPNE"
     gene            complement(484..>1568)
                     /locus_tag="PK791_06580"
     CDS             complement(484..>1568)
                     /locus_tag="PK791_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HOH93013.1"
                     /translation="GVKMFETSPRLEIYLERIKQNAQSVAKLCHDNGIQIAMVTKVLR
                     GNPEVARVFIEAGADMIADSRVPNLRLVGKHLPGVPRLLLRMPAQKQAEDVVMHTEYS
                     LNSSLMTLKALSDAAVKLGKVHKPIIMVDVGDLREGVWPENLVPLIKEAAMLPGLDLV
                     GLGANLACYGGVIPSVENMQILIDVRDEARRVTGLELGLLSGGNSSGLPLLASGKMPK
                     EINHFRVGEAIVLGRNVLDRNPWQGTRQDTIQVVAEIIELLEKPSVPIGDTGQDAFGN
                     EPEFEDRGIRKRAILNLGRQDVVIEGLEPVDPGIIVLGGSSDHAILDVSDAHTPLKLG
                     DEVSFYPGYGATLAAATSEYVRKVTI"
BASE COUNT          381 a          411 c          362 g          414 t
ORIGIN      
        1 gcaaactcgc caaaaacaag cgggtattgc accgtttagt ccacatgata ttcgccgtac
       61 gacgattacc gatatgctaa gcgcggatgt ggatgtgctt acggtgtcag ccatcgctgg
      121 acatgccagc gcagatacca cgcgtcgtta cgacaagcgt ggcgatgaaa gcaaacgcga
      181 agccgcccga aaaattcaat cgccttttaa agggcattaa gaaaagctaa ttattcgttt
      241 ggtttactgc taaataattc ctcgatgtcc tcacccatct ttttgaaggt gcttttcatt
      301 tcctcaccga actttttaat cttcgggtcg ttcttgcctt cgttccaggc tttttcaaaa
      361 gaatctccga cgtctgaaaa gctttgtttc agttcggcca ttaaattttt agcccagtta
      421 gggtcaaatt ctttttcgtc gttttcgggt ttatgatcca tatttctcct tgtcagactt
      481 ttattaaatc gtgacttttc tgacgtattc agacgttgcg gcagccaaag tggcgccata
      541 gccgggataa aagctaactt catcgcccag tttgagtggc gtgtgagcat cactgacgtc
      601 caaaatagca tggtcgcttg aaccacccag aacgatgatg cccggatcga cgggttccaa
      661 gccttcaatg accacatctt gtctgcccag gttcaaaata gcgcgcttgc ggataccgcg
      721 gtcttcaaat tcgggttcgt tgccaaacgc gtcctggcca gtatcgccaa tcggcacaga
      781 aggtttctcc aaaagttcaa tgatttcggc cacaacctga atcgtatcct ggcgagtgcc
      841 ttgccatggg ttgcggtcaa gcacgttgcg ccccagcaca atcgcctccc ctaccctaaa
      901 gtggttgatt tctttgggca tcttgccact cgccaaaagg ggcaaaccgg agctgttgcc
      961 accggagagc aaaccaagtt ccaaacccgt aacacgtcgg gcttcatccc gtacgtcgat
     1021 caggatttgc atattctcca cactgggaat aacgccccca taacatgcca ggtttgcgcc
     1081 caaaccgacc aggtcaagcc caggcagcat ggcagcttct ttgatgaggg ggactaaatt
     1141 ctcaggccaa acgccttcgc gcaggtcgcc gacatccacc atgatgatcg gtttgtggac
     1201 tttgcccagt tttacggcag catcggacag tgcctttaag gtcattagag atgaattaag
     1261 cgagtattct gtgtgcatga caacgtcttc agcttgcttt tgcgcaggca tgcgcaacaa
     1321 aaggcgcgga acgccgggca aatgtttgcc aacaaggcgc aagttgggca cacgcgagtc
     1381 ggcgatcata tccgcaccag cttcaatgaa aacacgtgcg acttccgggt ttccacgtaa
     1441 aaccttagtt accatggcaa tttgaatgcc gttatcgtga cacaatttgg caacactctg
     1501 agcgttttgt tttatacgtt caagataaat ctctagtctg gggcttgttt caaacatttt
     1561 tactcctt
//
LOCUS       DAOSFC010000290         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_82323_length_1565_cov_3.939735, whole genome shotgun sequence.
ACCESSION   DAOSFC010000290 DAOSFC010000000
VERSION     DAOSFC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82323_length_1565_cov_3.939735"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1400
                     /locus_tag="PK791_06585"
     CDS             156..1400
                     /locus_tag="PK791_06585"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOH93014.1"
                     /translation="MTVNKMLDNKRLEVNAEPPYDALQAFVRENHIALKGSGTGELNG
                     LTFAAKDVFKIRGSTFGNGHPDWLRTHEPDDFTSSTIQRLLDNGADLVGKTVCDELCF
                     SISGENWNYGSPLNPHDPRRFTGGSSSGSGAATSGGLVDFALGSDCLGSVRVPASYTG
                     VFGVRPTYDRVPSDGEAEYCPSMDVFGYMASDPEVFRKVSKVILGEDPEPVKLRKLII
                     AKDCFDAVSPQVFADLKPAIDHIGANLSSVEEREISPDGLDNWIKVFQQVQGYEVWRS
                     YGGWVRKYRPILSRGPRERLAWASTITLQEYNAGLVKMREISRYVRDMLANGEVLVMP
                     TAASVAPLRSAPLEEINATRLQSTYLLCVSPLSGVPQMTLPMVMADGLPLGLSLLSGF
                     NTDLALANLSADLVKSYRASIK"
BASE COUNT          412 a          334 c          394 g          425 t
ORIGIN      
        1 ctcggacatc ttctcagcga ttttggtagc agacagtgaa aacgaaattg accgtaactg
       61 ttcaggctta ccgcggagaa tccggactat gtccgccctg aagctgcgga ataagagtag
      121 agcaagtaaa ggaataaatt ctattcataa gtgatatgac agtcaataaa atgctcgaca
      181 acaaacgact ggaagtaaat gcggagccac cgtatgacgc gcttcaagcc tttgtgcgtg
      241 aaaaccacat cgctttgaaa ggctcaggca caggcgagtt aaacggtctg acttttgcag
      301 ccaaagacgt tttcaaaatt cgtggcagca cttttggaaa tggtcatccg gattggctcc
      361 gcacacacga accagatgac ttcacatcgt caacgattca acgcttgctt gacaatggcg
      421 ctgatttagt cggaaaaacg gtgtgtgatg aactttgctt tagtatcagt ggcgaaaact
      481 ggaattatgg ttcacccctt aatccccatg acccacgacg ctttacggga ggttcatcaa
      541 gtggatctgg tgcggcaact tcaggtggtt tggttgattt tgcattgggg agtgattgtc
      601 ttggcagtgt tcgcgtgcca gccagctata ccggcgtatt cggtgtacga ccgacctacg
      661 accgtgttcc aagtgatgga gaagcagaat actgccctag catggatgtc tttggctata
      721 tggcatctga cccggaagtt tttaggaagg tttccaaagt cattctggga gaagatcccg
      781 aaccagtcaa attacgcaaa cttattatcg caaaagattg ttttgatgcg gttagtcccc
      841 aagtgtttgc agacctgaaa ccagctattg accatatcgg tgctaatctc tcttcagttg
      901 aagaaagaga gattagccca gatggtttgg ataattggat taaggttttt cagcaagttc
      961 agggctatga agtttggcgt agttatggtg gatgggtgcg caaatatcgc ccgatcctct
     1021 cgcgtggtcc acgggaacgc ctggcatggg cttcaacaat aacactgcag gaatacaatg
     1081 caggtttggt caagatgcgt gaaatttcca ggtatgtgcg tgatatgctt gccaatgggg
     1141 aggttttggt aatgcctacc gccgcatcgg tggcaccttt gcgatcagcg ccactggaag
     1201 aaattaacgc aacgcgcttg caatcgacct atctcttgtg tgtctctccg ttaagtgggg
     1261 tgccacaaat gactttgccg atggtgatgg cagatggttt gccgttgggt ctgtccttgt
     1321 taagcggatt caacaccgac ctggcactgg ctaatctttc cgctgacctg gtgaagtctt
     1381 acagggcgtc cataaaatag aaacacttgc tttaatgtca gttttgcgag tagggtacaa
     1441 accctgctcg cttttttata gcttcaatac cttttttatg cctggatatc ttaaaaagaa
     1501 caaaatttgt ggggaaaatt tccagaaata cccttactaa aaaatagatt tacggctata
     1561 atggt
//
LOCUS       DAOSFC010000291         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_83880_length_1547_cov_3.875335, whole genome shotgun sequence.
ACCESSION   DAOSFC010000291 DAOSFC010000000
VERSION     DAOSFC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83880_length_1547_cov_3.875335"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PK791_06590"
     CDS             <1..944
                     /locus_tag="PK791_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOH93015.1"
                     /translation="SIADIIFNPDQVKSSWVYIFRNYRLVKAIAAVLSGMALGVAGLQ
                     MQTLFRNPLADPFILGINSGASLGVALVVMGTGVLSGAFISGLTTLGDISLAVAASIG
                     AGLVLGLILLVSRWVRNVTTLLILGLMFGYATNALVSLLIYFSRPDRVHAFMIWSYGS
                     FGGVTWNQLRVMAPVILIGIGLVMFLPKALNVLLLGEDYAKTMGLNVKKTRFLILISS
                     SLLAGTVTAFAGPIGFLGIAVPHIARNLLRTSDHKKLIPATLLLGAIVALVADMISQL
                     PGSHYILPLNVVTSIFGAPFVIWVILRQRRGETSFVV"
     gene            1031..>1547
                     /locus_tag="PK791_06595"
     CDS             1031..>1547
                     /locus_tag="PK791_06595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH93016.1"
                     /translation="MELCPGELICLVGPNGSGKSTLLRTITGLQKALAGDVLLHGQPI
                     ESYDNKALAKVISVVLTTPVQIGAMRAYDLVGLGRFPFTNMFDQMREEDHEVVRIALE
                     MAGATPLAERFVHTLSDGERQKVMIARALAQEPSLLMLDEPTAFLDLPGRVSVMQLLR
                     DLAHGHDKAILT"
BASE COUNT          313 a          330 c          448 g          456 t
ORIGIN      
        1 agagtatagc agatattatc tttaatcccg accaggtaaa gtccagctgg gtctatattt
       61 tcaggaatta ccgcctggtg aaagccatcg ccgcggtgct ttcgggtatg gcgctgggtg
      121 tggcaggttt gcagatgcaa acgcttttcc gcaacccgct ggctgacccc tttattttag
      181 gcatcaactc gggcgcgagc ctgggtgtgg cattggttgt catgggcaca ggggtcttga
      241 gtggtgcctt tatttcgggc ttgaccacat tgggtgatat ttccctggct gtggcggcat
      301 cgataggcgc gggactcgtt ttgggcttga ttttgctggt aagtcgctgg gtacgcaatg
      361 tgacgacctt gctgatttta ggtttgatgt tcggctatgc caccaacgct ttggttagcc
      421 tgttaatcta ctttagccgc cctgatcgcg tgcatgcttt tatgatttgg agttatggct
      481 cttttggtgg cgtgacctgg aatcagttgc gggtaatggc accggtgatt ttgattggta
      541 tcggtctggt gatgttttta cctaaggcgc tcaatgtttt gcttttgggc gaggattatg
      601 ccaaaacgat gggcctgaac gtgaagaaaa cccgtttctt gattttgatt agctcatcac
      661 tcctggcagg gacggtgacg gcatttgccg gtccgattgg ttttttgggc atcgctgtgc
      721 cgcatatcgc gcgcaatttg ttacgcactt ctgatcataa aaagcttatc ccagccactt
      781 tgcttttggg agccattgta gcactggtgg cagatatgat ttcgcaactt ccgggcagtc
      841 attatatctt gccgctgaac gtggtcacgt ccatctttgg cgcgccattc gtgatctggg
      901 tgattttgag acaaaggcga ggtgagacat cgttcgtggt ttaagcgcgc cggtattaat
      961 tgccaaagac cttgatattg gctataaaag aggcaatcag attgaaaaag ttgtcagtac
     1021 cgggctgaat ttggaacttt gtcccggtga actgatatgt ttggttggac cgaatggttc
     1081 gggcaagtca acgcttttac gcacgattac tggtttgcaa aaggccctgg cgggtgatgt
     1141 tttactgcat gggcagccta tcgaaagcta cgacaacaag gctttggcta aggtcatcag
     1201 cgttgtgcta acgacgcctg ttcagattgg cgcgatgcgc gcctatgatt tagttggttt
     1261 agggcgcttt ccttttacca atatgtttga ccaaatgcgc gaggaagacc atgaagtggt
     1321 gcggattgcg cttgaaatgg ctggtgcgac accattggca gagcgttttg tgcacacact
     1381 tagcgatgga gaacggcaaa aggtgatgat cgcgcgcgct ttggcgcagg aaccatcttt
     1441 gttgatgctt gatgagccga cagctttttt ggatttgccg ggacgggtct cggtgatgca
     1501 acttttgcgc gatttggcac acgggcacga caaagctatc ctgactt
//
LOCUS       DAOSFC010000292         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_84044_length_1545_cov_6.058389, whole genome shotgun sequence.
ACCESSION   DAOSFC010000292 DAOSFC010000000
VERSION     DAOSFC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84044_length_1545_cov_6.058389"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PK791_06600"
     CDS             complement(<1..448)
                     /locus_tag="PK791_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH93017.1"
                     /translation="MDAIRELEIVVNTFLQAGGPVLQTIMQAITFLGDELFYILFMPA
                     IYWCVSAWSGLRIGVMLLLSGGVNSIFKLALRGPRPSWISDKVTPVVHETSFGIPSGH
                     AMNSISVWGWAAVEAKKKWATISCIALIVLISFSRLVMGVHFLSDVI"
     gene            complement(619..870)
                     /locus_tag="PK791_06605"
     CDS             complement(619..870)
                     /locus_tag="PK791_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH93018.1"
                     /translation="MMDSSMYGKIDKAKRYAEERERIKFHSFEALIEGNNSDKTVRYN
                     DGKWDCDCHYFSNYGVCAHTMAFEILLEKMVVPGEHPEF"
     gene            complement(1128..>1545)
                     /locus_tag="PK791_06610"
     CDS             complement(1128..>1545)
                     /locus_tag="PK791_06610"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOH93019.1"
                     /translation="QALKFTLLSISAAGIEAVSFIILERLTTLPYDIKHVISIVLSVL
                     WNFTLNRRYTFKSANNVPIAMLKVGLFYAFFIPLTAWLGKLADGAGVNEYIIKGSTMV
                     LNFVGEFLWWKFIVFRGSENTNALAKKKQETNNETD"
BASE COUNT          450 a          341 c          333 g          421 t
ORIGIN      
        1 ggatgacgtc ggaaaggaag tgcaccccca tcaccagccg tgaaaatgaa atgagtacga
       61 tgagggcgat acagctaatc gttgcccatt ttttcttggc ttcaacggca gcccagcccc
      121 aaaccgagat ggaattcatg gcgtggccag atggaatgcc aaacgatgtt tcatgcacga
      181 ctggtgtaac cttgtcgcta atccaggatg ggcgcggtcc gcgtaaagcg agtttgaaga
      241 tgctgttcac gccaccactt aatagtaaca tgacgccgat acgtaagccc gaccaggcac
      301 tcacgcacca gtaaatggca ggcataaaaa gaatgtagaa aagttcatca cctaaaaagg
      361 tgatagcttg catgatggtt tgcaaaactg gtccaccggc ttgcaaaaag gtattaacaa
      421 caatttcaag ttctctgata gcgtccataa gttctccaaa ttatcttaaa aaggttcgtg
      481 catttaactt gtcgctaaac cacatagggt caagctatta aaaataataa cacttgatat
      541 cagcttaagt ctcaattcgc caaaagcatg gtcatgaacc cccaaagatg ctttttgaca
      601 taattgtaaa ttaccagatt aaaactcggg atgttcgccc gggacaacca ttttttcaag
      661 aaggatttcg aatgccatcg tgtgcgcgca aacgccgtaa ttgctaaaat aatggcaatc
      721 gcagtcccat tttccgtcgt tataacgcac ggttttgtcg ctgttattgc cttcaataag
      781 ggcttcaaaa ctatgaaatt ttatacgttc gcgttcctcg gcataacgtt ttgctttgtc
      841 aatttttccg tacatgctgc tgtccatcat cttaaaactc gctttctatg cttttatttt
      901 aggatttaaa aaaacacgcg ccaagaaaaa ggcgtgtgtc ttacggtgct tttacaagca
      961 gggtattcgc tttcataggt attaggctaa ctcctgcgtc atgtttctat tataccgcaa
     1021 agttatttaa cccgtttaaa ataaactctt cacatctaat tcctggatct tgaaaaagta
     1081 atttcaagaa gaaactgatg ggaggtcggc tttctactat cgatttatta atcagtctcg
     1141 ttatttgttt cctgcttttt ctttgccagg gcgtttgtgt tctcgctgcc cctgaaaacg
     1201 ataaacttcc accacaaaaa ttcgccgaca aagtttagaa ccatggtgct gcccttaatg
     1261 atgtactcgt taacaccagc cccgtcagct aatttaccaa gccaggctgt aagcggaata
     1321 aagaacgcgt agaaaagccc aaccttcagc atggcgatgg ggacgttgtt agctgatttg
     1381 aaggtatagc ggcggttgag ggtaaagttc cagagcactg agaggacaat ggaaatgaca
     1441 tgcttaatat cataaggcag ggtggtcagt ctctccaaga tgataaacga gaccgcctca
     1501 atccccgcgg cggaaatgga aagcaaggta aatttgaggg cttgt
//
LOCUS       DAOSFC010000293         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_84083_length_1545_cov_2.452349, whole genome shotgun sequence.
ACCESSION   DAOSFC010000293 DAOSFC010000000
VERSION     DAOSFC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84083_length_1545_cov_2.452349"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PK791_06615"
     CDS             complement(<1..801)
                     /locus_tag="PK791_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH93020.1"
                     /translation="MMNFSEILRSGLSTGTILIFAAIGEIIAERSGIQNLGVEGMMLF
                     GAMAAFKAAYMTGNVWIALLVAILAGGLLSLAHALVTIHFQADQTVSGLSLTFLGTGL
                     ALVIGEGLTGLTTVTVPNLTIPLLSKIPYIGPIFFTNLNLLVYVGYLTIPIAWHFINK
                     TRPGLHMRAVGENPAAADTLGVNVYGNRYFYTFVGGCMAGLAGATISVAVSPGWYSHL
                     TTSGQGWIAVALVIFSHWNPTRAAIGGYLFGMLRRFALDLQGPTMLLGL"
     gene            complement(798..>1545)
                     /locus_tag="PK791_06620"
     CDS             complement(798..>1545)
                     /locus_tag="PK791_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH93021.1"
                     /translation="MALLGMIGGALYGFIPGVLKAKFNINEIIVTLMLNYIVVFWNNY
                     WIFGKWSDAGFQMTKTFSKTAWLPRLADYATQVKAFSGITLHAGVLFGLIAALIVHII
                     LNHSNWGYMINLIGDNPRAARYAGADIAKNTILVFMLSGAFAGLAGMVEVSGVVHRLQ
                     ERISVSYGFSGVIVAWLAKLNPWAVIVVSILFGGLIVASREIQPSGISFLLQGIILFL
                     VISSDVLLKYKFQIVRSGKNVKTVNEEVSA"
BASE COUNT          454 a          393 c          383 g          315 t
ORIGIN      
        1 caagcccaaa agcattgtgg gaccttgcaa gtccaaagcg aagcggcgca acatgccaaa
       61 caagtagcca ccaatcgcag cgcgggtggg gttccagtgt gaaaaaatca ccaaagccac
      121 cgcgatccag ccttgccctg aagtcgtcag atggctatac cagcccggcg agaccgcgac
      181 actaatcgtc gcgccagcca agccagccat acaaccgccc acaaaggtgt agaaataacg
      241 gtttccgtaa acattcacgc ccagcgtatc agccgcggct gggttttcgc ctaccgcgcg
      301 catatgcaag ccaggtctgg ttttgttgat aaagtgccat gctatgggaa tcgtcaggta
      361 accaacataa accaagaggt tcaaattggt gaagaagatg ggtccgatgt aagggatttt
      421 tgagagcaga ggaatggtca aatttgggac tgtaacggtg gtgaggccag tgagaccttc
      481 gccgatgacc agggctaaac cggtgcctaa aaaggtgagc gatagaccgc taacggtttg
      541 gtctgcctgg aagtgaatgg taaccaaggc atgggctaag ctaaggagac caccagcgag
      601 aatcgccacc aggagcgcga tccatacgtt gccggtcata taggcggctt taaaagcagc
      661 catagcgcca aaaagcatca taccctcaac gcccaggttt tgaatgccac tgcgttccgc
      721 aataatttcg ccaatcgcgg cgaagatcaa aatggtgccg gttgagagac cagagcgaag
      781 gatttctgaa aaattcatca tgctgacacc tcttcattga ccgtcttgac atttttaccg
      841 gatctgacaa tttggaattt atacttcagc aagacatcac tgctgatgac cagaaacagg
      901 ataatgcctt gcaataagaa ggaaatgcca gaaggttgga tttcccgact ggcgacaatc
      961 agaccgccaa acaatatcga tactacaatc accgcccatg gatttagctt tgccagccat
     1021 gccacaatga cgcccgaaaa accatacgaa accgagatgc gctcttgcag acgatgcacg
     1081 acaccgctga cttcaaccat accagctaag cctgcgaacg cgccagagag cataaagacc
     1141 aggatggtgt tcttggcgat atccgcgcca gcatagcgtg cggcacgtgg gttatcacca
     1201 atcaggttaa tcatatagcc ccaattgctg tggttgagga tgatgtgcac aatcagcgcg
     1261 gcaatcagac cgaacaaaac cccggcatgg agtgtgatgc ctgaaaaagc cttaacttgg
     1321 gtagcgtaat ctgctaagcg tggcagccag gctgtttttg agaaagtttt ggtcatctga
     1381 aaacccgcgt cactccattt gccaaaaatc cagtaattgt tccaaaaaac aacgatgtaa
     1441 ttgagcatta aggtgacaat aatctcgttg atgttaaact tggctttcaa gacacctggg
     1501 atgaagccat aaagcgcgcc accaatcatg cccagcaaag ccatc
//
LOCUS       DAOSFC010000294         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_84199_length_1543_cov_4.614247, whole genome shotgun sequence.
ACCESSION   DAOSFC010000294 DAOSFC010000000
VERSION     DAOSFC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84199_length_1543_cov_4.614247"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PK791_06625"
     CDS             complement(<1..326)
                     /locus_tag="PK791_06625"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOH93022.1"
                     /translation="MDKTLRLIVVLILALVVLGACYVFPSREIRDGEQGTLFSETKTL
                     DRYSKIDFEGGGKIVLVQGNSHSLKIEASQAVLRKIVVKQDGETLHVGFTGNVWQMSA
                     TDELIFT"
     gene            complement(483..884)
                     /locus_tag="PK791_06630"
     CDS             complement(483..884)
                     /locus_tag="PK791_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOH93023.1"
                     /translation="MEVPRFWRLKKQRYALQGEVCPHCDVKIFPPRDVCPACGGEAKT
                     PFTFSGLGEVYSFTDIKNAPAGFEEQAPYTVALVQLKEGPMVSAQLTDLGDEPVHIGM
                     SVEMVTRKIRNVGNDRGIIAYGYKFRPVMVK"
     gene            complement(1050..>1543)
                     /locus_tag="PK791_06635"
     CDS             complement(1050..>1543)
                     /locus_tag="PK791_06635"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH93024.1"
                     /translation="LFKIGSLQFQPFEMLYLDKEGIGRDIMRFTETQKLRLPPGMPVL
                     NTHIQHGADLRPEALRATFAQARDFFRRYFPSQDFKAFISYTWLLYPGLNEVLPAESR
                     ILQFAEMWDIVSVVDDKTQALERIFGGRRRRKADYPQESSLQRLALRHMDALGYALGM
                     KEF"
BASE COUNT          417 a          387 c          334 g          405 t
ORIGIN      
        1 gtaaaaataa gctcatcggt cgctgacatt tgccatacgt ttccagtaaa gccaacatgc
       61 agtgtttcac catcttgctt gacgactatt tttctcaaca ccgcctggct ggcttcaatt
      121 ttcaaactat ggctgttacc ctgaaccaag acaatcttcc cgccaccttc aaagtcaatc
      181 ttgctatatc ggtcaagcgt tttggtttca ctaaacaagg tgccttgctc accgtctctg
      241 atttcgcgac tggggaacac ataacaagca ccaagcacca cgagcgccag tattaacaca
      301 acaatcaatc ttaacgtttt atccatataa tcctccgaaa cgaacatatc tcattattaa
      361 tgatagagct tttgtattag ctgtcactta aggttttgaa aaggacactt aacaaaacct
      421 tagaaaaaac cgacccgcag ggagtcggct gagtacacag tacgcatttg atgcacttca
      481 tcttatttga ccatgacagg tctaaacttg tagccatagg caatgatgcc acggtcgttg
      541 ccaacattgc ggattttgcg ggtgaccatc tccaccgaca tgccgatatg cacgggttcg
      601 tcgcctaaat ccgtcagttg cgcgctcacc atggggcctt ctttgagttg caccagcgcc
      661 accgtgtaag gggcttgttc ttcaaaacca gcgggcgcgt ttttgatatc ggtaaaagaa
      721 tagacttcgc ccaaaccgct gaaagtgaag ggggtctttg cctcaccacc gcaagccgga
      781 cagacgtcac ggggtgggaa tattttgaca tcgcaatgcg ggcagacttc gccctgcaaa
      841 gcgtaacgtt gttttttcaa tcgccaaaag cgtggaactt ccataaaatc tcctttgcaa
      901 tagctgcaat ggtcttagtt tatagcaaga aaactatcaa gaactatcag atttggtatt
      961 tttgggccaa ttttgggtgt tttagtggca aaattgcctg tttttgaaag atgtttgtcg
     1021 agataccggt ttgaagtaag aagcgaaact taaaattcct tcatgcccag cgcgtagccc
     1081 agggcatcca tgtgacgcag tgccaagcgt tgcaaactgc tttcctgcgg gtaatcggcc
     1141 ttgcgacggc gccggccacc gaatatccgc tccagtgcct gggtcttgtc atccacaaca
     1201 gagactatgt cccacatttc agcaaactgt aatatcctgc tttcggctgg caaaacttca
     1261 tttaaacccg gatacaaaag ccaggtgtag ctaatgaagg ctttaaaatc ctgcgatggg
     1321 aaataacgcc tgaagaaatc ccttgcctgc gcgaatgttg cccgcaaagc ctctggccgc
     1381 aaatccgcgc catgttgaat atgcgtgttc aatacaggca ttccaggtgg caagcgcaac
     1441 ttttgcgtct cagtgaagcg cataatatcc ctgccgatgc cttctttatc cagataaagc
     1501 atttcaaagg gctgaaactg taaagaacct atcttaaaaa gct
//
LOCUS       DAOSFC010000295         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_85110_length_1533_cov_3.472936, whole genome shotgun sequence.
ACCESSION   DAOSFC010000295 DAOSFC010000000
VERSION     DAOSFC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85110_length_1533_cov_3.472936"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PK791_06640"
     CDS             complement(<1..353)
                     /locus_tag="PK791_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH93025.1"
                     /translation="MSDWQNLSEQLRKLGVKLGVQEQEKAKPPQTDKFPIESVLNCSE
                     TRTPAGNIISIHKQFPLSYTHGAHNITPSDSFKRILTWANIPEQKNIKLENLIFLDTE
                     TTGLSGGTGTMVFMVG"
     gene            complement(350..1441)
                     /locus_tag="PK791_06645"
     CDS             complement(350..1441)
                     /locus_tag="PK791_06645"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOH93026.1"
                     /translation="MLNQQNNKYYFISDDYPAANVSLRATTPNIVTLKCVTDQHTQTI
                     GEIDASSASWFVHPGAIYLHDADTYKVNALDIDKGECLLEAISTDYYTIPNLSTSIEN
                     FSEITQEDHQNYQSNYGNLSLKFEINSFKKLRWHTNEVLGVESLNLPPSELTTVGFWI
                     SLKQGFLNQLKDDGKWTATDNDYGTNWQKLREKIIARDNYRCRACGKSFASASLHVHH
                     IQPFKSFGDPQLANKASNLVTLCASCHHLAEINVRIRSGLAAATNALRNLAPLLIMCD
                     AEDIGCLAEPKSVLANGLPAILVYDGMPGGLGLSRKLYEEQSFWFTRATEAIESCPCA
                     EGCPACVGPVGEQGYGGKAEGIALLKGLI"
BASE COUNT          349 a          333 c          396 g          455 t
ORIGIN      
        1 ccaaccatga agaccatcgt acccgtaccg cctgacaatc cggttgtttc cgtgtcaaga
       61 aaaattaggt tttcaagctt gatattcttt tgctctggga tgtttgccca ggtcaatatg
      121 cgtttaaatg aatcgcttgg ggttatatta tgcgcgccat gcgtatagct tagcgggaat
      181 tgtttgtgta tagaaatgat gttgccagcg ggggttcgcg tttcgctgca atttaggacg
      241 gactcaattg gaaatttgtc tgtttggggt ggcttggctt tttcttgttc ctgaacgcca
      301 agcttgacgc cgagtttgcg taattgttcg cttaggtttt gccagtcgct catatcagtc
      361 ctttgagcag tgcgatgcct tcagctttgc caccgtagcc ttgttcgcca actggtccaa
      421 cgcaggcggg gcagccttcg gcgcaagggc agctttcaat ggcttctgtg gcgcgtgtga
      481 accaaaagct ttgttcttca tacaatttac gggatagtcc taatccgcca ggcatgccgt
      541 cgtagaccaa aatggcaggc aatccattgg caaggacgga ctttggttca gcaagacagc
      601 cgatatcctc tgcatcgcac atgattaaga gcggcgccag attgcgaagt gcgtttgtcg
      661 cggcggcaag tccactgcga atacgcacgt taatttctgc cagatgatga cagcttgcgc
      721 aaagagtaac cagattgctg gctttgttgg cgagctgagg gtcgccgaag gacttgaaag
      781 gttgaatatg atggacatgc aggctggcac tggcgaagct tttcccgcac gcgcgacacc
      841 gatagttatc gcgtgcgatg attttctccc gcagtttttg ccagtttgtg ccatagtcgt
      901 tatccgtcgc ggtccatttg ccatcatctt taagttggtt caggaagcct tgctttaggc
      961 taatccaaaa accgactgtt gtgagttcac tgggtggcag gttgagtgat tcaactccga
     1021 gtacttcgtt ggtatgccag cggagttttt tgaaactgtt tatctcaaat ttcagggaga
     1081 gattcccata attgctttga taattttgat gatcttcttg cgtgatttcg gaaaagtttt
     1141 ctatgctggt actcaggttg gggatggtgt aataatctgt gctaatagct tcaagcaaac
     1201 actcaccttt atctatatca agcgcgttta ccttataagt gtccgcgtca tgcaaataaa
     1261 tcgcgccagg atgcacaaac caggatgctg aagatgcgtc aatttcgcct attgtttgag
     1321 tgtgttgatc tgtaacacat ttgagggtta cgatattggg cgttgtcgcg cgtagtgata
     1381 catttgccgc gggatagtca tcgctgatga agtagtactt gttgttttgt tggttaagca
     1441 ccgcctgctg agagaacaat tccaaaatgc tgcgtaataa atagggtcaa ccttgccaaa
     1501 tacttcatcc gcgctaaagg gtaattcaaa gct
//
LOCUS       DAOSFC010000296         1506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1789
            NODE_87488_length_1506_cov_3.771192, whole genome shotgun sequence.
ACCESSION   DAOSFC010000296 DAOSFC010000000
VERSION     DAOSFC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863318
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:20:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,330
            CDSs (total)                      :: 1,304
            Genes (coding)                    :: 1,301
            CDSs (with protein)               :: 1,301
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87488_length_1506_cov_3.771192"
                     /isolate="AMR_MDS_1789"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1506
                     /locus_tag="PK791_06650"
     CDS             <1..>1506
                     /locus_tag="PK791_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH93027.1"
                     /translation="AYPAAMKPMLLPAEQMEGPKGKEQRNYISAAVYWGNELGVEGFM
                     TNIRDIVANLINTLIYILLSARLEWKWLPLILLPAVGQGIMSLIFMKQINEKNQERQN
                     LWAEGHYMQSIALKNEAAKDVRLYPIVDIFKKKYLDLRKRWHGYVSGKQYRLFAADAS
                     FWLLSIARDVIVFIVLFRRVQAGMPVSDFVLYMGVILGISAYVSGIFNKIALLFQNNF
                     VVTHFREAMQQPEYDQTGTPGLVPQGLGTIRFENVSYSYSSENENTKWALKNINLTIK
                     PGEKIALVGENGAGKTTLVKLATGIFTPTRGEIYYDGINVKEFKPTELYKKVAMVFQN
                     VTIIATTLAENITCQPESEIDYERLKWAIEKAGLSEVVARLSEGVHSQLTTYLHKDGV
                     NLSGGENQRLMLARALYKGGDILILDEPTAALDPLAEAALYERYHELAENQTAVFISH
                     RLSSTQFCDRVVFLKEGEIVETGTHASLMEAKGAYQHMFHEQAKYYQTEAA"
BASE COUNT          417 a          331 c          380 g          378 t
ORIGIN      
        1 aagcttatcc cgctgcaatg aagcccatgc tgttacccgc cgagcaaatg gaaggcccca
       61 aaggcaaaga acagcgcaat tacatctctg ctgcggtcta ttggggtaac gaacttgggg
      121 tggaaggttt catgacgaat atacgggata ttgttgcaaa tctcatcaac accttgatat
      181 acattctgct ttcagcacgc ctggaatgga aatggctgcc tctgatcctc ttacctgctg
      241 tcgggcaagg cattatgtca ctcattttta tgaagcagat aaatgagaaa aaccaggaaa
      301 ggcaaaatct ttgggctgaa ggccattaca tgcaaagcat cgcgctgaaa aatgaggcgg
      361 caaaagatgt cagactgtac cccatcgtgg atatttttaa gaaaaagtac ctggacttgc
      421 gcaagcgttg gcatggctat gtgagcggaa aacaatatcg gcttttcgct gcggatgcaa
      481 gtttttggct gttgagcatc gcgcgcgatg tcattgtctt tatcgtcctt tttcgacgtg
      541 ttcaggcggg catgcctgtc tccgactttg tgctctatat gggcgttatc ttgggcataa
      601 gtgcttatgt gagtgggata tttaataaaa tcgcactttt gtttcaaaac aactttgttg
      661 ttacgcactt tcgcgaggcg atgcaacagc cggaatatga ccaaaccggc acgccaggtt
      721 tggttcctca aggtttgggt acgattcgct ttgagaatgt ctcttactcc tattcaagtg
      781 agaatgagaa tacaaaatgg gcgctaaaaa atatcaacct gacgattaaa cccggcgaga
      841 aaattgccct ggttggcgaa aatggcgcgg gcaaaacgac gctggtcaag cttgccacgg
      901 gcatcttcac accaacgcgt ggcgaaatct attatgacgg tatcaacgtg aaggaattca
      961 aacctacgga actttacaaa aaagttgcca tggtctttca gaatgtgact atcatcgcca
     1021 cgacattggc ggaaaacatc acttgccagc ccgaaagcga aattgactat gaacgcctga
     1081 aatgggcaat tgaaaaagcc ggcttgagcg aggttgtggc acgattaagc gaaggtgtgc
     1141 acagtcaact gacgacttat ttacacaaag acggggtgaa cttgtcgggt ggcgaaaacc
     1201 agcgtttgat gctggcgcgt gccttgtata agggcggcga catccttatt ctagacgagc
     1261 ccacagcggc actggatcca ttagctgaag ctgcattgta cgaaagatat catgaattgg
     1321 ccgaaaacca aactgcggtt tttatctcgc atcggctttc ttcgacccag ttttgtgatc
     1381 gggtggtctt tttgaaggaa ggcgaaatcg ttgaaaccgg cacgcatgca tctttaatgg
     1441 aagccaaagg cgcttatcaa catatgttcc atgaacaagc caagtactat caaacggagg
     1501 cggcat
//
